LOCUS       DASQGH010000001         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53810, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000001 DASQGH010000000
VERSION     DASQGH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53810"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VEW91_00005"
     CDS             <1..1491
                     /locus_tag="VEW91_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HYM90000.1"
                     /translation="VFASILGTWTVRVRGEGIGDPMRPINTGLWVSATAAIAGIAAMN
                     AAYLTDPNTGAPDWRFSSAAFTGLVLALVIKWLTDQFTNPNRRPVTEMAYSTKTGPAT
                     FLLSGLGFGLESSVWAILAVAATILASFTIFRGDLALAGYGVALAGLGLLSVTGYILA
                     MDTFGPIVDNANGIVEMSGAHKDSDLASRIVARLDQAGNTTKALTKGFAIATAVVAAT
                     ALFRSFVDQAHLTASVAGLRQALAAGGAAGAAALDHVGIQVNLPLVFIGLLIGGAVPF
                     LVSAFLIRAVGRSAFEVVEEVRRQFREIPGIMEGKAKPEYYRCVDIVTRAAQRELLPP
                     AVIAIAAPITVGFGLGVAALGGFLAGAILTGQLLAVFMANTGGAWDNAKKKIEDGFLG
                     GKGTEYHKAGVIGDTVGDPLKDTAGPALNPLIKVMNLVSLLVAPVTIAPLGWGPRGAV
                     VAVGLALAAAAVAMNRRSSIARRDLEAAARMAAQADDPAVPTGRSR"
     gene            1488..3173
                     /locus_tag="VEW91_00010"
     CDS             1488..3173
                     /locus_tag="VEW91_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYM90001.1"
                     /translation="MRKPPRAPARPSPPARTPGARERLLRKAAEISALREISRTISAA
                     TDLDSTLTLITRKTAEVMQMDSCSIYLIDTPGDYLVLKATTGLAPEAVGYARLHLGEG
                     LTGWAAEKGAAAASTNAAADPRFKYLPETHESLFQSLLAVPLTTGGKVLGAINVQTTG
                     SHEYTEDEVELLSIIADLASGALEKARLYDSMRRQIMELSTLAEVSETLTSPLYLEQI
                     LRLLVEMTARVFDAPLCTLMLLDGEELVLAAVHPESPLPRPRRTLRVGEGIPGVAAAS
                     GQPVASPDLSTDPRFRPHELPQPSGGTDVRSMLAVPLRVRDRTLGVVNCYTDRPHEFT
                     QNEVNLLQTLANQTALAIENAGLVVKSAMVREMHHRVKNNLQMIAMLLRLQLRDGRAV
                     SGREVLTETINRILSIAAVHEILAAEGLRQVAVRAMLERVVHTVTQTMAPPGFALDAR
                     IEGDDPSLPSQQATSLALVVNELLQNAMEHAFPGRSAGRVVITLTEGRDAIRVEVRDD
                     GVGLPEGFSPGRSADLGLQIVRTLVQEDLKGQLSLTNVRGVRAVITMPRPQGS"
     gene            3189..3737
                     /locus_tag="VEW91_00015"
                     /pseudo
     CDS             3189..3737
                     /locus_tag="VEW91_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT          767 a         1747 c         1809 g          729 t
ORIGIN      
        1 gtcttcgcct cgattctcgg gacctggacc gtccgggtcc gcggggaggg gatcggagac
       61 cccatgcggc ccatcaacac cgggctgtgg gtctccgcga ccgccgcgat cgcaggcatt
      121 gccgcgatga acgcagcgta cctcaccgac ccgaacaccg gggcaccgga ctggcggttc
      181 tcctcggcgg cctttaccgg gctggtgctt gccctcgtga tcaagtggct caccgaccag
      241 ttcacgaacc cgaaccgccg cccggtgacc gagatggcct actcgaccaa gacggggccg
      301 gcgacgttcc tattgtccgg gctgggcttc ggcctggagt cgtccgtctg ggcgatcctg
      361 gcggtcgcgg cgacgatcct cgcatcgttc acgatctttc ggggggatct cgccctggcg
      421 gggtacgggg tcgcgctggc cgggctgggg ctcctcagcg tgacgggcta catcctggcg
      481 atggacacct tcggaccgat cgtcgacaac gccaacggca tcgtggagat gtccggggcg
      541 cacaaggaca gcgacctggc ctccaggatc gtggcgcggc tcgatcaggc cggcaacacg
      601 acgaaggcgc tcacgaaggg gttcgcgatc gccaccgcgg tggtcgcggc gacggcgctc
      661 ttccggtcgt tcgtcgatca ggcgcacctc accgcctccg tggcgggcct ccgccaggcc
      721 ctagccgcgg gcggggccgc gggggccgcc gcgctcgatc acgtggggat ccaggtgaac
      781 ctgccgcttg tcttcatcgg gctgctgatc ggcggggcgg tcccgttcct cgtctcggcc
      841 ttcctcatcc gggcggtggg gcgatcggcg ttcgaggtcg tcgaggaggt ccgccggcag
      901 tttcgcgaga tcccggggat catggagggg aaggcgaagc cggagtacta ccggtgtgtg
      961 gacatcgtca cccgggccgc ccagcgtgag ctgctcccgc ccgcggtcat cgccatcgcc
     1021 gcgccgatca cggtcgggtt cggcctgggg gtcgccgccc tcggcgggtt cctcgccggc
     1081 gcgatcctca ccgggcagtt gctcgcggtg ttcatggcga acaccggcgg ggcgtgggac
     1141 aatgccaaga agaagatcga ggacgggttt ctgggcggga aagggaccga ataccacaag
     1201 gcgggggtca tcggcgacac ggtcggcgac ccgctcaaag acacggcggg gccggcgctc
     1261 aaccccctga tcaaggtgat gaacctcgtt agcctcttgg tggcgcccgt gacgatcgcc
     1321 ccgctgggct ggggcccccg cggcgcggtc gtcgcggtgg ggctcgcgct cgcggccgcc
     1381 gccgtcgcaa tgaaccgcag gagctcgatc gcccgtcggg atctggaggc ggccgcccgc
     1441 atggccgccc aggcggacga tccggcggtc ccaaccggca ggtcgcggtg aggaaaccgc
     1501 cgcgcgctcc cgcacgacct tcgccgccgg cccggacgcc cggcgcgagg gagcggctgc
     1561 tccggaaggc cgctgagatc tcggcgctgc gcgagatcag ccggacgatc agcgcggcga
     1621 ccgatctgga ctctacgctg acgctgatca cccgcaagac ggccgaagtg atgcagatgg
     1681 acagctgctc gatctatctg atcgacaccc cgggcgatta cctggtcctc aaggccacca
     1741 cgggcctcgc gccggaggcg gtggggtatg cccggctcca tctgggcgaa ggcctgaccg
     1801 ggtgggcggc cgagaagggc gcggccgcgg cgtcgaccaa cgcggcggcc gatccccgct
     1861 tcaagtacct gcccgaaacg cacgagtccc tcttccagtc gctgctggcg gttcccctga
     1921 ccacgggcgg caaggtcctc ggcgcgatca acgtccaaac gaccgggtcg cacgagtaca
     1981 ccgaggacga ggtcgagctc ctcagcatca tcgccgatct cgcgagcggg gcgctcgaga
     2041 aggcccggct ctacgacagc atgcggcgtc agatcatgga gctgagcacg ctggccgagg
     2101 tgagcgagac cctgacgtcc ccgctctacc tggagcagat cctgcggctc ctggtggaga
     2161 tgaccgcgcg ggtcttcgac gcccccctgt gcaccctcat gctgctggat ggggaggagc
     2221 tggtgctcgc ggcggtgcat ccggagagcc cgctcccgcg gccccgccgg accctgcgcg
     2281 tgggcgaggg catccccggg gtggccgccg cctccggcca gccggtcgcg agcccggatc
     2341 tctcgaccga cccccggttc cgcccgcacg agctgcccca gccctccggg gggaccgatg
     2401 tgcggtcgat gctggccgtc cccctccggg tgcgcgaccg caccctgggc gtcgtgaact
     2461 gctacaccga tcgcccccac gagttcaccc agaacgaggt caacttgctc cagacgctgg
     2521 cgaaccagac cgcgctcgcc atcgagaacg ccgggctcgt ggtcaagtcg gcgatggtcc
     2581 gcgagatgca ccatcgcgtc aagaacaacc tgcagatgat cgcgatgctg ctccgcctcc
     2641 agctccgtga cgggcgggcg gtctcgggcc gggaggtgct gaccgagacg atcaaccgga
     2701 tcctgagcat cgcggccgtg cacgagatcc tcgcggcgga aggcctccgc caggtggcgg
     2761 tgcgggcgat gctcgagcga gtggtccaca ccgtgacgca gacgatggcg ccgccggggt
     2821 tcgcgctcga cgcacgcatc gagggggacg atcccagcct gccgagccag caggccacgt
     2881 cgctcgcgct ggtggtcaac gagctgttgc aaaatgcgat ggagcacgcc ttccccgggc
     2941 ggtcggcggg acgggtcgtg atcaccctga ccgaggggcg ggatgcgatc cgggtggagg
     3001 tgcgggacga cggcgtgggg ctgcccgaag gcttctcccc cggccggtcc gccgatctgg
     3061 gattgcagat tgtccggacc ctcgtgcagg aggacctcaa aggtcagctc tcgctgacca
     3121 acgtccgggg cgtgcgggcc gtgatcacga tgccgcgtcc gcaggggagt tgatccgtgg
     3181 ccgacgcgat gcgcatcctg atcgccgacg atgaacccgt gatccgcatg ggcctcaaga
     3241 cgatgctcga ggagcacggg tacaaggtcg tgggggaggc ctcggacggc gagcaggccg
     3301 tgaccctcgc ggggaagacc tcacccgatc tcatcttcat ggatatcaag atgccggggc
     3361 tcgacgggat cgccgccgcg tccacgatca tgacccgggc ccccaaaccg atcatcctgc
     3421 tcaccgcctg gagcgagcgc gatctggtgc agcgggcgca ggaggcgggg gtgctggcct
     3481 acctggtcaa accggtgcgg gaggccgagc tgatcccggc gatcgaagtg gcgatggccc
     3541 ggtttcagga gctccgcgcg ctccagcagg aggtcggcac actcaaggag acgctcgaga
     3601 cccgaaagct cgtcgagcgc gccaagggca tcctgatgaa gcgcgagggg gtcgatgaac
     3661 cggaagcgtt cctccgcatc cagcgccaga gccggaacac caggacgccg atgcgggaga
     3721 tcgcccgggc catcctcgtc tccgacgagc tccgccggca ctcggcggcc acggcggccg
     3781 gacggatcga cgcgcgcacg ccggcggatt tcttgcgcgc gctgagcgcc gtccgcgtct
     3841 tcgccgggtc cccgggaggg ccggacctct ccgtgtcggg ggcggagccg ctggcctacc
     3901 tgccccccgc gcccggcaac gagatcacgc tcgagggata cgtgacgggg cggacccagg
     3961 acggcgcgta cggcctcggc atcgccttcg ggatcgcgcc agcgccatcc cctgtcgagt
     4021 ccgagggcat agtggcgtgc gtcattcaaa acgcgcttcg cccggcggcg ctcgccgccg
     4081 gggggacagg gcgcagcgtc tacgcccaac tcctggatga ggtgatcgca acccagcagc
     4141 gcgtgaccgt gaccggcctg ctgcgattgc tccccgagca cgcctcggtc gggtccccca
     4201 cggttccgca cctgctggag attcacccgg tgcggagtgt catgaccgaa cgcgggctcg
     4261 cgttcccccc ggtcccgctg gacgcccccg ggggcgcgga gtggaccgag atcgagaccg
     4321 tgcatcccat gccgtttgcc gacgcccagc cgcgcacgaa aatggcggtc accggagcgg
     4381 tgctgacgtt tcgagatgtc cccccggtgg aaaccgccta cgtgtatatg accggacggt
     4441 ttcacggcgg gcgcagccgg ttcgtggagg gccgcccgtt cctctttgcg ttcggcgacg
     4501 ccgaagggcg gacccggctc actgcggtga ccatcccggg gacgcccgcc tatgagatct
     4561 cgcggcgctg gttgtccact ccccccacgg gcccgatcac ggtcgtgggg ctccgcacgc
     4621 tctacctgcc ggccctgttc gccccgggcg ggggaaggat cgaagtgctc ctctgcccgg
     4681 tgtttcgcat cgtcccggga acggtccggg gagcgtcaac cccggcgccg accacccgat
     4741 acgaactgat ccccgtcggc ggcatcccgc gcatcgccga gggccggcca ggtgcgcggg
     4801 gcaggggccc gaaggggcgg cccgggaagc ggacgcgggc ggcacgggtc ccgagcgccc
     4861 gggtccgaaa ggcgcgaacg aagaagcccc agcgggcccg ggggcgccgg gcgtcgaagg
     4921 gccgaagtcg ccggcgataa gatgcatcta aaagtctgtg atggagggaa gcgccagggc
     4981 ccaccgtagg ggtaggtgac cacaccaaac cctggaggtg agccctggtg aaggagatcg
     5041 tgctcccctt cc
//
LOCUS       DASQGH010000002         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_25503, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000002 DASQGH010000000
VERSION     DASQGH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25503"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VEW91_00020"
     CDS             <1..241
                     /locus_tag="VEW91_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90002.1"
                     /translation="SGVAFADQPDTKVWEIRLGVGHGSTFGTVSGAPQVNGIAGQLQY
                     NFAPHLFLQGTVLYQVAGNVPGGAFGFVILGYQFP"
     gene            532..1890
                     /locus_tag="VEW91_00025"
     CDS             532..1890
                     /locus_tag="VEW91_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HYM90003.1"
                     /translation="MDITKVVLAEDHIPKAWYNIRADLPVAPAPVLHPGTRQPISPTD
                     LEPLFPPEIILQEVSTERYVDIPREVYDIYRLWRPTPLYRARRLEQALHTPAHIYYKY
                     EGVSPAGSHKPNTAVAQAFYNKQAGVRRLATETGAGQWGSALALASRLLGLECTVYMV
                     RVSFEQKPYRRVMMETWGAEVLPSPSPRTAAGRAVLERDPQSLGSLGIAISEAAEDAA
                     THGDAKYSLGSVLNHVLMHQTVIGLEAKSQMEAFGEYPDVVIGCCGGGSNLAGLSFPF
                     LADTFKGKSLRVVAVEPTACPTLTRGKYLYDFGDEAETSPLLKMYTLGHKFVPAGIHA
                     GGLRYHGDAPLICLLYAHKYIEAVAYPQIPVFAAAVQFARTEGILPAPESAHAILKAI
                     DEALEAKEAGEARTILFNLSGHGHFDLGAYQDYVAGQLKDYAYPEAEVEKAMAGLTPL
                     QP"
BASE COUNT          364 a          678 c          670 g          395 t
ORIGIN      
        1 cagtggcgtg gcgtttgccg atcagccgga caccaaggtt tgggagatcc ggctcggggt
       61 tggacacggg tccacgtttg gtacggtgtc gggcgcccca caggtgaacg gcatcgccgg
      121 ccagcttcag tacaatttcg cgccgcatct cttcctgcaa ggcaccgtgc tctatcaggt
      181 cgcgggtaat gtgccaggcg gagctttcgg gttcgtcatc ctcggttatc agttcccata
      241 gatccctttt atcatgggcc ttttctgacg gatttccgcg gccaccatcc tcggcggcta
      301 cctccacgtc tgcgggattc cagaaattgg gggaaacatg attaagggtt tccgtgaacc
      361 tgccgcttcc gacttcccgt ttggtacctt actggtgcga tgggcatctg acacgcgcct
      421 tggcgtgccc cccgggcgtg ggaaaccgag ggcaggggat ggaggttgtt gtcccaaagt
      481 tcttagaagc gctccgtggg acgcatgcgt gtgtgcgatg ggaggggctg aatggacatt
      541 accaaggtcg ttctcgcaga ggatcacatc ccaaaggcct ggtacaacat tcgggccgac
      601 cttccggtcg cccccgcgcc ggtgcttcac cccgggacca ggcagcccat ctcccccacg
      661 gatctcgagc ccctcttccc tccggagatc atcctccagg aagtcagcac ggagcgctat
      721 gtcgacattc cccgggaagt ctatgacatc taccggctgt ggcgtcccac gccgctctac
      781 cgagcccgcc gcctggagca ggccctccac actcccgcgc acatctacta caagtacgag
      841 ggggtgagcc ccgccggcag ccacaagccg aatacggcgg tggcccaggc cttctacaac
      901 aagcaggccg gcgtgcggcg gctcgcgacg gagaccgggg cggggcagtg gggaagcgcc
      961 ctcgcccttg cctcgcgcct gcttgggctt gagtgcaccg tgtacatggt tcgggtcagc
     1021 ttcgagcaaa aaccctatcg ccgggtgatg atggaaacgt ggggcgcgga agttctcccg
     1081 agccccagcc cacgcactgc cgcggggcgc gccgtgctcg agcgagaccc gcagtctctg
     1141 gggagcctgg ggattgccat cagcgaggcg gcggaggatg cggccacgca cggcgacgcc
     1201 aagtattcgt tggggagcgt cctcaatcac gtgctcatgc accagacggt gatcggcctg
     1261 gaagccaaga gtcagatgga ggcgtttggg gagtatccgg atgtcgtcat cggctgctgc
     1321 ggaggcggga gtaacctcgc agggctgagc tttcccttcc tggccgacac gttcaagggg
     1381 aaatcccttc gggttgttgc ggtcgagccg acggcctgcc cgacgctgac gcgagggaag
     1441 tacctctacg attttggaga cgaagccgag acctccccgc tcctcaagat gtacacgctc
     1501 gggcacaagt tcgtcccggc cgggatccat gccggaggcc tccgctatca tggggacgcc
     1561 ccgctcatct gcctcctcta cgcccacaag tatatcgagg ctgtggcata cccccaaatt
     1621 ccggtcttcg cggcggccgt gcaattcgcc cggacggaag gaatcctgcc cgccccggag
     1681 tcggctcacg cgatcctgaa ggccatcgat gaggcgctgg aggccaagga ggccggggaa
     1741 gcgcgtacga tcctcttcaa cctgagcggg cacggccact tcgacctcgg cgcctaccag
     1801 gactacgtcg ccggccagct caaggactat gcctatccgg aggccgaagt cgagaaggcc
     1861 atggcggggt tgaccccact gcagccctga aggcgagtcc tgttttgcgg tcgcccggcg
     1921 cctgcggcca caggttcctt ggatcggtgg ggtggccgcc gatctccagg cggacgaagt
     1981 ggtcttcctc ctcgtttgcg ttcgtgcgcg gcgcctcgaa gtcgaaattc tccagcccga
     2041 tgtcggcccc ggcatgtcga agtctagcac atgcatccat ctccagcgaa cagggattct
     2101 tgcccgc
//
LOCUS       DASQGH010000003         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2850, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000003 DASQGH010000000
VERSION     DASQGH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VEW91_00030"
     CDS             <1..925
                     /locus_tag="VEW91_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HYM90004.1"
                     /translation="GQTIGLISPSGPTSATTATTPEILERTRARLAEAGFRTVIGAHA
                     LETRGYLAGTDAGRAADLHAMFADPNVHAVMCIRGGYGAMRVLPALDLDLVRRNHKVF
                     IGYSDITALHLAFHRRAGIVTFHGPMAHAVAQDNGHDLVQLLRAVTRADPLGPLANPP
                     GGPTVETLVPGTAEGVLIGGNAALLASLLGTPYEPEFEGALLFLEDIVDHPYQFDRSL
                     AQLRLAGVLDAVTGIVVGECRLRAERAEPPDQTLTIREVLDDLIGPLGKPAIYGLACG
                     HGTYHLTLPIGVRARLDAARKVLSIEEAAVT"
     gene            1129..1758
                     /locus_tag="VEW91_00035"
                     /pseudo
     CDS             1129..1758
                     /locus_tag="VEW91_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
BASE COUNT          281 a          654 c          670 g          330 t
ORIGIN      
        1 gggacagacc atcggcctga tctcgccgtc ggggcccacc tccgcgacca ccgccacgac
       61 cccggagatc ctcgaacgga cgcgcgcgcg gcttgcggag gcgggattcc ggaccgtcat
      121 cggcgcccat gccctggaga cccggggcta tctcgcggga acggacgccg ggcgggcggc
      181 ggacctgcac gcgatgttcg cggatcccaa cgtgcacgcc gtgatgtgca tccgcggggg
      241 ctacggggcg atgcgggtgc tgcccgcgct ggacctcgat ctcgtgcggc gcaaccacaa
      301 ggtgttcatc gggtacagcg acatcacggc gctccacctc gccttccacc gccgtgccgg
      361 gatcgtgacg tttcacgggc cgatggcgca cgccgtggcc caggataacg gacacgacct
      421 ggtccagctc ctccgggccg tcacgcgcgc cgacccgctc ggcccgctcg ccaacccgcc
      481 gggcggccct acggtcgaga cgctcgtccc cggcaccgcc gaaggcgtgc tgatcggcgg
      541 gaacgcggcg ctgctggcct ccctcctcgg gacgccgtac gagcccgagt tcgaaggggc
      601 cctgctcttc ctcgaggaca tcgtggacca cccctaccag ttcgacagga gcctcgcgca
      661 gctccggctc gcgggggtgc tcgatgcggt caccggcatc gtggtcgggg agtgccgcct
      721 gcgggcggag cgagcagagc ctcccgatca aacgctcacc atccgggagg tcctcgatga
      781 tctgattggt ccgctcggca agccggcgat ctacggcctg gcgtgcgggc acggcacgta
      841 ccacctcacg cttcccatcg gggtccgggc gcggctcgac gcggcccgca aagtgttgtc
      901 catcgaagaa gcggcggtga cctagatggt gctgcttcgg agccctttcc aggtggcggg
      961 gactcgccag caaggggccc tggaatggcc gggaccggtg atccgcgaaa tctcggaggt
     1021 gcttgaggtt ctcgagagcg tccgcgccgc gccgtgaacc tgccaacatg ctagtcctcg
     1081 ctgtccgttg cgcgggatcg aatcgtctgg aggtgacgtg ggacggagcc aaggaccgca
     1141 ccattcgaaa ctcatcaccg tcgctacgag gactggttcg aacgccacac gcctgcctac
     1201 tactcggaac tgctcgcgat ccgcgccttc cttccctgga gcggccgggg gctcgagatc
     1261 ggggtcggca gcggtcgctt cgccgccccg ctcggcgtgg gagtcggcgt tgacccctca
     1321 ggggcgatgc tggcgtacgc gcgctcgcgc ggggtgcagg tggttcaggg catcgccgag
     1381 gcgctgccct tcacggacgc cgtctttgcc tatgcgttga ttgtcaccac tatctgtttc
     1441 gtgagtgatc cgagggccat gctcgcagag actcgccgtg tgctgaggcc cgccggccgg
     1501 ctcgtgatcg gcttcatcga ccgggagagc gccctcggcc aatcctattt gtcccgccag
     1561 agagagcgcg tcttctaccg ggacgcgacc ttctactcgg ccgaggaggt cgaagccctg
     1621 gtgctaagcg cagggttcac cggactggtg tgggggcaga cattgtcccg acctctcgag
     1681 accctgcagg atgtcgagcc gctccgtgcc gggcggggcc agggtgcctt tgtcgtgatg
     1741 caggccacgc gtccgtagcg agtgtggtgc gccttgggat gtttcttgct gcggcggggg
     1801 ggcatcctcc cgctatcaaa ggcatagaag atcagggggt tcggtccctt gctcagccgt
     1861 cggcgacgtg tggaatcgga ggggatgccg atggatcgcc gatcagattt cttaccgctg
     1921 cgcatgcggc cccca
//
LOCUS       DASQGH010000004         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50988, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000004 DASQGH010000000
VERSION     DASQGH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50988"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VEW91_00040"
     CDS             complement(<1..785)
                     /locus_tag="VEW91_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYM90005.1"
                     /translation="MRRFACAAIRMGILATCLLLPWGAAGAQSVPPAPPVLTLDQAIQ
                     QALAQNAQLLAARAAVIAAQQNLAVARTGLAPTISASGTGTFGTSSSTSTTFGGLSAP
                     LTSVTGTGSVSLAATLPLFDGGRTQANVQAAEAAVTSAQAALRQTEQDNGLSVATAFF
                     SVLQAEHLAAVQEALLLQAQAQLALAQARARAGVAAQSDVIQAQAPVAQARVNLLAAR
                     SQIGTTKAVLQGAIGVDPAGPIEVQEPPAPPSAVTATAEAVMQA"
     gene            complement(785..1960)
                     /locus_tag="VEW91_00045"
     CDS             complement(785..1960)
                     /locus_tag="VEW91_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM90006.1"
                     /translation="MRSLRAKLSAAFILIAILQTVLMATFASYQAHRAFRQYVERTTE
                     RDGRAAVVGAPGAAIAVPPQGPIRRPPYGAREVLFSSRIRNAIWGATLIAAGLGVLVA
                     LWLVSRIGKPVAELTRATRAIARGGPAPLVPATGRDEMAELGQAFNHMAAQLAEEEQQ
                     RRRLFAGIAHELRTPLAVIQGTLEGMLDRVIEPTPERIAGLHSQAVLLKRLITDLRDL
                     SLAQAGQLRLHRRTIDVAGVVRETLEALAPLAGERTVALRVEVPAHLPPVHADPDRLR
                     QVVQNLVENALRHTPAGGEVRITLQDGNGDGVYDGVYMVVADTGSGIRAEDLPHIFEH
                     FYRADASRARSSGGTGMGLAIVKSLVEAHGGRVSVESAPGAGSTFTVTLPRAPQEAS"
     gene            complement(1953..2645)
                     /locus_tag="VEW91_00050"
     CDS             complement(1953..2645)
                     /locus_tag="VEW91_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM90007.1"
                     /translation="MPRILVVDDEPQIVDLLRSYLRRDGFDVDEAGDGEAALTACAHL
                     RPDLVILDLMLPKVDGREVCRRIRETSHTPIIMLTARDEETDKLLGLELGADDYITKP
                     FSPREVVARVRAVLRRGSRETVEMVRAGDLVIDLRAHEVSLEGRRVDLTPTEFRLLET
                     LASHPNQVFTRMQLIDRVQGHAFEGYERTVDAHIKNLRGKVELDPKNPRYILTVYGVG
                     YKFQTAAEDHHA"
     gene            complement(2653..>2850)
                     /locus_tag="VEW91_00055"
     CDS             complement(2653..>2850)
                     /locus_tag="VEW91_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HYM90008.1"
                     /translation="MWIVGGIFLLLRALLLIGLVVLVWRVLRAPTLWQRRDSAAQILR
                     ERYARGEIGEDEFRKRLGTLA"
BASE COUNT          415 a         1044 c          974 g          417 t
ORIGIN      
        1 gcctgcatca ccgcctcggc ggtggccgtt accgcggacg ggggcgcggg cggctcctgg
       61 acctcgatcg gcccggcggg atcgacccct atagcgccct ggagcacggc tttcgtcgtc
      121 ccgatctggg agcgcgcggc aagcagattc acccgcgcct gggccaccgg cgcttgggcc
      181 tggatcacgt cggactgcgc ggccaccccg gcgcgcgccc gcgcttgggc aagcgcgagc
      241 tgggcctggg cctgcaacag cagcgcctcc tggaccgcgg ctaagtgctc agcctgcaag
      301 acgctgaaga acgcggtcgc caccgacagc ccattgtcct gctcggtctg gcgcagcgcc
      361 gcctgcgcgg aggtcaccgc ggcctccgca gcctggacgt tcgcctgagt gcggccacca
      421 tcgaacaacg gcagggtggc cgcgagggag accgaaccgg tcccggtgac gctggtgagg
      481 ggggccgaca ggccgccgaa ggtggtcgac gtcgacgagg aggtgccgaa ggtgcccgtg
      541 ccgctggccg agatcgtcgg cgcgaggccc gtgcgcgcca cggcaaggtt ctgctgtgcc
      601 gcaatgaccg ccgcccgcgc ggccagcaac tgagcgttct gcgccagcgc ctgctggatg
      661 gcctggtcca gggtgagcac gggtggcgcg gggggcacgc tctgcgcccc cgccgctccc
      721 caggggagta gcaggcaggt ggccaggatc cccatccgga tggctgcaca cgcaaaccgc
      781 cgcatcacga cgcctcctgc ggggctcgcg gcagggtgac ggtgaaggtg ctgccggcgc
      841 ccggcgcgct ctcgacgctc acgcgccccc cgtgcgcctc caccagcgat ttcacgatgg
      901 ccagccccat ccccgttccc ccgctgctgc gtgcccgcga cgcatcggcc cggtagaagt
      961 gctcgaagat gtggggcagg tcctcggcac ggatgccgct accggtgtcc gcaacgacca
     1021 tgtatacccc atcatatacc ccatcgccat tgccgtcctg cagcgtgatg cgcacctcgc
     1081 ctcccgctgg ggtatggcgc agagcgttct ccactaggtt ctgcaccacc tgacgcagcc
     1141 gatccgggtc cgcgtgcacc gggggcaggt gcgcggggac ctccacccgc aacgcgacgg
     1201 tccgctcgcc cgcaagcggg gccagggctt ccagcgtctc gcggaccacc ccggcgacgt
     1261 cgatcgtccg ccggtggagc cgcaactgcc cggcctgcgc gagtgagaga tcccgcaggt
     1321 cggtgatcaa ccgcttcagc aacaccgcct ggctgtgcag cccggcgatc cgctccgggg
     1381 tcggctcgat cacgcgatcc agcatcccct ccagcgtccc ctgaatcacc gccagcgggg
     1441 tgcgcagctc gtgtgcgatg ccggcaaaga gccggcgccg ctgctgctcc tcttcggcca
     1501 gttgggcggc catatggttg aaggcctgcc ccaattccgc catctcgtcc cgtccggtgg
     1561 cgggcacgag cggcgcgggc ccgccgcggg cgatggcccg cgtggcccgt gtcagctccg
     1621 ccaccggctt tccgatgcgg ctgaccagcc acagcgccac gagcacgccc agcccggcgg
     1681 cgatcaacgt ggcgccccag atggcatttc ggatccggct ggagaacaaa acctcccgcg
     1741 cgccgtaggg gggccggcgg atcggaccct gcgggggaac ggcgatcgcc gcccccggcg
     1801 cacccaccac cgccgcgcgc ccgtcgcgct ccgtggtccg ctccacgtac tggcggaacg
     1861 cgcggtgcgc ctggtagctg gcaaacgtcg ccatgagcac ggtttgcagg atcgcgatga
     1921 ggatgaacgc ggcactgagc ttggcgcgca ggctacgcat ggtggtcctc ggccgccgtc
     1981 tggaacttgt accccacgcc atacaccgtg agaatgtagc ggggattctt gggatcgagt
     2041 tccaccttcc cccgcagatt cttgatgtgc gcgtccacgg tgcgttcgta cccttcgaac
     2101 gcgtggccct ggacgcggtc gatgagctgc atgcgcgtga agacctggtt gggatggctg
     2161 gccagggtct ccagcagccg gaactccgtc ggggtgaggt cgacgcgccg gccctccagg
     2221 ctcacctcgt gcgcccgcag atcgatgacc aggtcaccgg cccgaaccat ttcgaccgtc
     2281 tcccggctcc cgcggcgaag cacggcgcgg acgcgcgcca ccacctcgcg ggggctgaag
     2341 ggcttcgtga tgtaatcgtc ggcccctaac tccagcccca gcaacttgtc cgtttcctcg
     2401 tcgcgtgccg tgagcatgat gatcggcgtg tgcgaggtct cgcggatgcg gcggcacacc
     2461 tcccggccgt ctaccttggg cagcatcagg tccaggatga cgagatcggg gcgcaggtgc
     2521 gcgcaggcgg tcagcgccgc ttccccgtcg cccgcctcat cgacgtcgaa cccgtcccgc
     2581 cgcaggtagg agcgcagcag gtccacgatc tgcggctcgt cgtcgaccac gaggatcctg
     2641 ggcacgggtc ggtcacgcca gcgtcccgag gcgcttgcgg aattcgtcct cgccgatctc
     2701 gccccgggcg taccgctccc gcaggatctg ggctgcgctg tcccggcgct gccacagggt
     2761 gggggcgcgc agcacgcgcc agaccaggac cacgaggccg atcaacagca gggcccggag
     2821 caacaggaag atgcctccca cgatccacat
//
LOCUS       DASQGH010000005         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28339, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000005 DASQGH010000000
VERSION     DASQGH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28339"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VEW91_00060"
     CDS             complement(<1..667)
                     /locus_tag="VEW91_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYM90009.1"
                     /translation="MRRSWIGIAFVVAALIVGLVIGRGTSPAPVGRPASADPPSSAPS
                     KSKTPPPPLVGVANPKRVTIQNTLSLTANIGSLKTAVIYSKTAGYLETVTVRAGDPVR
                     TGQVVAVVDHAQLDAQVSQARATLLAAQSGIQTAKAGVATAHAQLANARAGVVKARAQ
                     LQDAEATESRIAALVRQGAVAQQSLDDATAQTQSARASLDAAAAQVTAAREQENAAGT
                     QVQT"
     gene            complement(667..1128)
                     /locus_tag="VEW91_00065"
     CDS             complement(667..1128)
                     /locus_tag="VEW91_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYM90010.1"
                     /translation="MTMFYQIGEVCQRTGLTQRALHYYDEIGLLVPSERLNGGQRLYA
                     AADLERIGRIKNLKQLLGLSLGEIKRLLDADEARAKYLSAAQTASDPARRRKAVEDAL
                     QVTADQLQSVREKVHQLGRLQRQLERQVRELRRVTTPAGPSRAPAAIGDRP"
     gene            complement(1253..>1582)
                     /locus_tag="VEW91_00070"
     CDS             complement(1253..>1582)
                     /locus_tag="VEW91_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90011.1"
                     /translation="PAAPTGGWPAARFAPPTARLPESPGGYPAMVARLQRYAAEAGRD
                     PRAIGLERRINFASGPGEWGRASGEWSRVGGTHLSVNTMRAGLPSARAHIDAAQQVWR
                     ELHRNLG"
BASE COUNT          197 a          579 c          546 g          260 t
ORIGIN      
        1 gggtctgcac ctgcgtcccc gccgcgttct cctgctctcg agcggcggtg acctgggcgg
       61 cggcggcgtc caggctcgcc cgggcgctct gggtctgcgc ggtggcgtca tcgaggctct
      121 gctgcgccac ggcgccctgc ctgacgaggg ccgcgatccg gctctcggtg gcctcggcgt
      181 cttgcagctg ggcccgtgcc ttcaccaccc cggccctggc gttcgccagc tgcgcgtggg
      241 cggtcgcgac ccccgccttc gcggtctgga tgccgctctg ggcggccagc agcgtggccc
      301 gcgcctggga gacctgggcg tcgagctggg cgtggtcgac cacggcgacg acctgccccg
      361 ttcgcacggg atccccggca cgaaccgtca ccgtttccag ataccctgcg gtcttcgagt
      421 agatcacggc cgtcttgagc gagccgatgt tggccgttag cgataacgtg ttctggatcg
      481 taacacgctt cggattagcc acccccacca gcgggggcgg tggggtcttg cttttggacg
      541 gcgccgacga cggtgggtcc gcgctggccg gtcgcccgac aggcgccggg gaggtccccc
      601 gcccgatcac cagccccacg atgagggcgg cgaccacgaa ggcgattccg atccaggacc
      661 ggcgcatcag gggcgatctc ctatggcggc cggggcgcga gacgggcctg ccggcgtggt
      721 gacgcgccgg agttccctga cctgccgctc cagttggcgc tggagccgcc ccagctgatg
      781 caccttctca cgtacggact gcaactggtc cgcggtcacc tgcagcgcgt cctccaccgc
      841 cttgcggcgg cgcgcgggat ccgacgccgt ctgggccgcg ctcaggtact tggcccgcgc
      901 ctcgtcggcg tcgagcaggc gcttgatctc gccgagcgaa aggccgagca gctgcttgag
      961 gttcttgatg cgcccgatcc gctcgagatc cgcagcggcg tacagccgct gcccgccgtt
     1021 caaccgctcg ctggggacca ggagcccgat ctcatcatag tagtgcaggg cccgttgggt
     1081 gagcccggtc cgctggcaga cttccccgat ctgatagaac atcgtcatct ggtgccctcg
     1141 ctccccaccc tcgagtgtcc cgaacgcagc acgcgtacca ccatcttaga ggcaccttac
     1201 gtgcgtgtca ataaccttcc gtgtacgtga gggtccggcc ggaacgcggt ggctagccaa
     1261 ggtttctgtg gagttcgcgc cacacctgct gagccgcgtc gatatgcgcc cgggccgagg
     1321 gcagccccgc gcgcatcgtg ttcacgctga ggtgcgtgcc cccgacccgg ctccactcgc
     1381 cggatgcgcg cccccattcg ccggggccgc tcgcgaaatt gatccgccgc tccagcccaa
     1441 tcgcgcgcgg atctcggccg gcctcggccg cgtaccgctg cagccgcgcg accatggccg
     1501 gatacccgcc cgggctctcg ggaagccggg cggtgggggg tgcgaagcgt gccgccggcc
     1561 atccacccgt cggcgcggcg gg
//
LOCUS       DASQGH010000006         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17014, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000006 DASQGH010000000
VERSION     DASQGH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17014"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VEW91_00075"
     CDS             complement(<1..395)
                     /locus_tag="VEW91_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90012.1"
                     /translation="MRGRPSSVAKRVIAGLSAVLLLATGAGASLSPREILHRVLTANA
                     GTPDVSSADAQFKLRVKKSLTDPPDCEFEGTMQLQGGHQSLRVGRHTAGLLCWAVDQY
                     VLGQLFEASEPLANFLKRFEFRVLGEKLVG"
     gene            552..2444
                     /locus_tag="VEW91_00080"
     CDS             552..2444
                     /locus_tag="VEW91_00080"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HYM90013.1"
                     /translation="MIRDVSPAMRTYRTLALAAAGGAYLLILLGGLVRISGAGLACPD
                     WPLCHGRLIPPLEGVVLIEYAHRLVAAAVSLLVVATTLYAFRVRGVAPRAGTTALWVL
                     ALVGLQIVLGALTVKLQLTPLLVTIHLGVANLFLAALLVQAVGAWQGPAAAPPGPSSF
                     RRLVGVTGAATYALILIGGYVASSGAGLACPDLPLCRGASIIPADAGAHFHMLHRGWA
                     LVVAGLVLATAGAASRVGGRSTVTAAYLAVGLVSMQLAVGVLNIVTRLAPLVRSAHLG
                     IATLLFGTLVVLGALGRPLPATATDALRIRKTRRALPTIAGGSIEESTRTLPWDAPGA
                     SASGLRAAAGVARDYAALMKPRIIALLLVTTATTMVMAAPHRLGLGVLLLTLLGGTLA
                     AGSANAFNMYVDRDIDAVMRRTCLRPVPAGRLRPGQALGFGVLTGLLSIGVMAWGVNA
                     LSAALSTAGIVFYVGVYTLWLKRTTTQNIVIGGAAGAVPPLVGWAAATGHIALPAGIL
                     FAIVFFWTPPHFWALALGKTDDYRAAGIPMLPVVRGAGETRRQILVYSIVLAATTLVL
                     YYPLHTLGGVYLAAAVILNGVFVALAAVVARGRTPWAPPALFGYSIVYLALLFGAMVA
                     DRLTLG"
     gene            2615..>2822
                     /locus_tag="VEW91_00085"
     CDS             2615..>2822
                     /locus_tag="VEW91_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90014.1"
                     /translation="MTDHTHQLKKVPLFADIPDAQLQRIANGVKERRFEPGASIVSTG
                     ETGHGFYLIVQGRAEVKRDGRTERT"
BASE COUNT          359 a         1024 c          966 g          473 t
ORIGIN      
        1 cccaccagtt tctcccccag cacgcggaac tcgaaccgct tgagaaagtt cgccagcggc
       61 tcgctggctt cgaacaactg cccgaggacg tactggtcga cggcccagca gagcaatccc
      121 gcggtgtgcc gtcccacccg cagcgattga tgcccgccct gcaactgcat cgtcccctcg
      181 aactcacagt cgggaggatc ggtgagcgac ttcttcacgc ggagcttgaa ctgagcgtcc
      241 gcagacgaca catcgggggt gccggcgttc gccgtgagca cgcggtggag gatctcccgc
      301 ggagacaggg aggcgcccgc gcctgtcgcg agcaacaaca ccgccgatag tcccgcgatc
      361 acccgcttcg cgacactcga tggcctaccc cgcacccagc tgttcatccc aggcctctaa
      421 tcgtactccc ttccgatatt cgagggcaat accctttcgg ttttccccaa accccgtatg
      481 ccccgctcat tcgatcgata gccggggcgt ctcgacagga gagaactgcg ccgcgcgtcg
      541 aaggaggcgg catgatccgt gacgtgtctc ccgcgatgcg aacgtaccgg acgctcgccc
      601 tcgccgccgc cggcggcgcg tacctgctga tcctcctggg cgggctggtc cggatcagtg
      661 gggccggcct ggcgtgtccg gactggccgc tctgccatgg gcgcctgatc ccgccgcttg
      721 agggcgtggt gctgatcgag tacgcgcacc gcctggtggc ggcggccgtg agcttgctcg
      781 tggtcgcgac caccctgtac gcgttcaggg tcaggggggt ggccccccgc gcgggcacga
      841 cagccctgtg ggtcctggcc ctcgttggcc tgcagatcgt cctcggggct ctgaccgtca
      901 agcttcagct caccccgctc cttgtgacca tccacctcgg cgtggccaat ctattcctgg
      961 ccgccctcct cgtgcaggcg gtcggagcct ggcaggggcc ggccgccgcc cctcccggtc
     1021 cctcctcttt ccggcggctg gtgggagtga cgggcgcggc cacctatgcg ctgatcctca
     1081 tcggcggcta cgtggcgtcg agcggcgcgg ggctggcgtg ccccgacctg ccgctctgcc
     1141 gcggcgcatc gatcatcccc gcggatgcgg gggcgcattt ccacatgctg caccgcgggt
     1201 gggcgctggt ggtcgccgga cttgtgctgg cgaccgccgg cgcggcctcg agggtcggtg
     1261 gacgctccac cgtgaccgct gcgtacctgg ccgtggggct tgtgtccatg cagctggccg
     1321 tgggagtcct caacatcgtg acccggctcg ccccgctcgt gcggagcgcg catctcggga
     1381 tcgccaccct gctcttcggg acccttgtcg tgctgggcgc cctgggcagg ccgctccccg
     1441 cgaccgccac ggacgcgcta cggatccgga agacgcggag ggcgcttccc accattgcgg
     1501 ggggctcgat cgaggagtcg acgcgcacgc tcccctggga tgctccgggg gcgtcggcct
     1561 cggggcttcg cgcggccgcg ggagtcgccc gggattacgc ggcgctgatg aaaccccgca
     1621 tcatcgcgct gctgctcgtc acgaccgcaa ccaccatggt gatggcggcg cctcaccgcc
     1681 tcgggctggg ggtgttgctg ctcaccctcc tcgggggcac gctggccgcc gggtccgcca
     1741 acgccttcaa catgtacgtt gaccgggaca tcgatgccgt gatgcggcgg acgtgcctgc
     1801 gcccggtgcc ggccggccgc ctgcgccccg ggcaggccct cggattcggc gtcctgacgg
     1861 ggctcttgtc catcggcgtc atggcgtggg gtgtaaatgc cctgagcgcc gccctctcga
     1921 ccgccgggat cgtattctac gtgggggtgt atacgctctg gctcaagcgg accaccaccc
     1981 agaacatcgt catcggcggt gccgctggcg cggtgcctcc gctggtcggg tgggcggcgg
     2041 ccaccgggca catcgcgctg ccggcgggca tcctgttcgc catcgtgttc ttttggacgc
     2101 cccctcactt ctgggcgctg gcgctcggca agacggacga ctaccgcgcg gccgggatcc
     2161 cgatgctgcc cgtggtgcgc ggggctggag aaacccgcag gcagatcctc gtctactcca
     2221 tcgtcctcgc cgcgaccacg ctggtgctgt attatccgtt gcacacgctg gggggcgtgt
     2281 acctggctgc cgcggtgatc ctgaacggcg tgttcgtcgc cctggccgcc gtggtcgcgc
     2341 ggggccggac gccctgggcg ccgccggccc tcttcgggta ctccatcgtc tacctcgcgc
     2401 tgctgttcgg cgcgatggtc gccgaccgcc tgactctcgg ctgacgggct cggcgcctcc
     2461 ccggcgccat catgcgcgcg ggccgcaagg ggaggaatcc cccgtgaccc cggccaaccc
     2521 cctagtcggc ccggatacga gtcgtccggc gcggcgccgc cggcccgggt ggaggccgcg
     2581 gggcgccgga tcctggcagt caggggggat cgccgtgacc gatcacaccc atcaactcaa
     2641 gaaggtgccg ctgttcgccg atatcccgga cgcccagctc cagcggatcg caaacggggt
     2701 caaggagcgc cggttcgaac ccggcgcgtc gatcgtctcg accggcgaga ccgggcacgg
     2761 cttctacctc atcgtccagg ggcgggccga ggtgaagcgg gacggccgca cggagcggac
     2821 cc
//
LOCUS       DASQGH010000007         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17016, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000007 DASQGH010000000
VERSION     DASQGH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17016"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VEW91_00090"
     CDS             <1..555
                     /locus_tag="VEW91_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYM90015.1"
                     /translation="VTEVDPLRALEAHMDGYRVLPMLEAARVSDVLLTVTGDTAVIRR
                     EHFEVMKDRAIVGNVGHFNVEIDLGALAELAAERRAVREHVEEFRLRDGRRIYLLAEG
                     RLLNLAAAEGHPAAVMDMSFANQALAIEHLAQHGRALAPDVYPVPAEIDRQVASLKLG
                     AEGITIDTLTPQQEHYLRSWQEGT"
     gene            573..1808
                     /locus_tag="VEW91_00095"
     CDS             573..1808
                     /locus_tag="VEW91_00095"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYM90016.1"
                     /translation="MDRAHTRDASSGGPGGALADRMGLLGTETAFEVLVRAKALEAAG
                     HSIIHLEIGEPDFDTPEHIKEAAIRALRDGYTHYTPAAGLREAREAIAEHVGALRGIT
                     VDPEEVVITPGAKPVMFFVMMALVNPGDEVIYPDPGFPIYESVARFVGGRPVPWVLRE
                     DREFRADPEELRALMTPRTKLIILNSPHNPAASVLARRDLEAIAEIVRGRPVTVLADE
                     IYGRILYEGAFASLASIREVRGQTVILDGFSKAYAMTGWRLGYGVMRPEFASRITQLM
                     VNSNSCTAAFTQIAGIAALRGPQDSVGRMVAEFRRRRDVIVDGLNALPGVTCLRPPGA
                     FYAFPNVRKIDPDSSRLQEYLLREAGVAVLSGTAFGAHGEGYLRLSYANSVEALTEAL
                     QRIRRAVSRYAPRSGAERR"
     gene            complement(1735..2136)
                     /locus_tag="VEW91_00100"
     CDS             complement(1735..2136)
                     /locus_tag="VEW91_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HYM90017.1"
                     /translation="MTVARLFVYGTLRNDTVVQGLLGHRLFGRPAVLRGYHREVDPAI
                     GYPVILPRAGATVAGKVLDVDADALAILDAYEGARYRRIIVQVETLEGRLLDAYVYVP
                     AALPARDPSAVPPPTGERSGSPPDGSAEAPR"
     gene            complement(2133..>2543)
                     /locus_tag="VEW91_00105"
     CDS             complement(2133..>2543)
                     /locus_tag="VEW91_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90018.1"
                     /translation="FETFMRLCEALNQSPAYFIEGYRDRPVKGRVDTPQDAVDALETK
                     SSQRHLELIHRLEHLPVEIGRSIAPEELRATLERMRCEDLPATVEARLGATLPQRHGG
                     RDDESTVAREAFRQGWEAAHEALRRWLHERGQAV"
BASE COUNT          390 a          856 c          915 g          382 t
ORIGIN      
        1 gtcaccgagg tggatcccct ccgcgccctc gaggcccaca tggacggcta ccgggtgctg
       61 ccgatgctgg aggcggcgcg ggtctcggat gtgctcctga ccgtgaccgg cgatacggcg
      121 gtgatccgcc gggagcactt cgaggtgatg aaggatcgcg cgatcgtggg caacgtcggc
      181 catttcaacg tcgagatcga tcttggggcg ctggccgagc tcgccgcgga gcgccgggcg
      241 gtgcgggagc acgtggagga gtttcgcctt cgagacggcc ggcggatcta tcttctggca
      301 gagggaaggc tcctcaacct ggccgccgcc gaggggcatc ccgcggcggt gatggacatg
      361 agcttcgcga atcaggcgct ggcgatcgag cacctcgccc agcacgggcg ggcgctcgcc
      421 ccggacgtct acccggtgcc cgcggagatc gaccggcagg tggcgtcgct caagctcggc
      481 gccgaaggga tcacgatcga caccctgacg ccccaacagg aacactatct gcggtcctgg
      541 caggagggca cctaggaaaa ggagggagca ccgtggacag ggcgcacacg cgcgacgcct
      601 ccagcggggg cccgggaggc gcgctggccg atcggatggg tctgctgggc accgagaccg
      661 cctttgaagt gctcgtccgg gccaaggcgc tcgaggccgc cgggcattcg atcatccacc
      721 tggagatcgg ggagccggat ttcgatacgc ccgagcacat caaggaggcc gcgatccggg
      781 cgctccgcga cggctacacg cactacacgc cggcggccgg gctgcgcgag gcgcgcgagg
      841 cgatcgccga gcacgtgggg gcgctgcgcg ggatcacggt ggaccccgag gaggtggtga
      901 tcacccccgg ggccaagccc gtgatgttct tcgtcatgat ggccctggtc aacccgggcg
      961 acgaggtgat ctatcccgac ccggggtttc cgatctacga gtcggtcgcc cggttcgtgg
     1021 gggggcgccc cgtgccctgg gtgctccggg aggatcggga gttccgggcg gaccccgaag
     1081 agctccgcgc gctcatgacc ccccgcacca aactgatcat cctgaactcc ccgcacaatc
     1141 cggcggccag cgtgcttgcg cggcgggacc tggaggcgat cgcggagatc gtccggggtc
     1201 ggcccgtgac ggtgctcgcg gacgagatct acggccggat cctatacgag ggcgcgttcg
     1261 ccagcctggc cagcatccgc gaggtgcgcg gacagaccgt catcctcgac gggttcagca
     1321 aagcgtacgc gatgaccggg tggcgcctcg ggtacggtgt gatgcgtccc gagttcgcct
     1381 cgcgcatcac ccagctgatg gtgaactcca actcctgcac cgcggcgttc acccagatcg
     1441 cggggatcgc cgcgctgcgc ggcccccagg acagcgtcgg ccggatggtg gcggagtttc
     1501 gccggcggcg ggacgtgatc gtggacggcc tcaacgcgct ccccggggtc acctgtctcc
     1561 gcccgcccgg ggcgttctac gcgttcccga acgtgcggaa gatcgatccg gacagctcgc
     1621 gcctccagga atacctgctc cgcgaagccg gggtggcggt gctgtccggc acggcgttcg
     1681 gcgcgcacgg ggaagggtac ctgcgcctga gctacgccaa ctccgtggag gcgctcaccg
     1741 aggcgcttca gcggatccgt cgggcggtga gccgctacgc tccccggtcg ggggcggaac
     1801 ggcgctaggg tcgcgtgcgg ggagggcggc tgggacgtag acgtacgcat ccaggagccg
     1861 gccctcaagc gtctccacct ggacgatgat gcgccggtag cgggcgcctt cgtaggcatc
     1921 caagatcgcc agcgcgtccg cgtccacgtc caacaccttt ccggcaaccg tggcgcctgc
     1981 ccgggggagg atcacggggt agccgatcgc ggggtcgacc tcgcggtgat accctcggag
     2041 cacggcgggg cgcccaaaca accggtggcc caggagaccc tggaccaccg tgtcattgcg
     2101 aagcgtcccg tagacaaata gccgcgcaac ggtcacacag cctgaccccg ttcgtgcaac
     2161 caccggcgga gcgcttcgtg ggccgcctcc cacccctggc ggaacgcttc ccgggccacc
     2221 gtggattcgt catcccgccc cccgtgccgt tgcggcaggg ttgcgcccag gcgcgcctcc
     2281 acggtcgcgg ggaggtcctc gcaccgcatc cgttcgagcg tcgccctgag ctcctcgggg
     2341 gcgatgctcc ggccgatctc aacgggaagg tgttcgaggc ggtggatcag ttcgaggtgc
     2401 cgctgtgacg atttcgtctc gagcgcgtcg acggcatcct gcggtgtatc cacgcgcccc
     2461 ttgacgggtc ggtcccgata cccttcgatg aagtacgcgg gcgattggtt gagggcttca
     2521 cacaaccgca tgaaggtctc gaa
//
LOCUS       DASQGH010000008         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17024, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000008 DASQGH010000000
VERSION     DASQGH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17024"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VEW91_00110"
     CDS             complement(<1..424)
                     /locus_tag="VEW91_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90019.1"
                     /translation="MTGPALVRSLAGAAVLVLVGAQGGLSAPVPVVPPAAVLRMALDA
                     PRVFDYEGTKIITVLRGGRTETITVAESHKRPDAVRLEYLSPEALAGRLIVDNGTITW
                     HYEPRLNMAFEGPTLGGEFQGRDLTVLLRNYRVTALGVE"
     gene            complement(421..933)
                     /locus_tag="VEW91_00115"
     CDS             complement(421..933)
                     /locus_tag="VEW91_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYM90020.1"
                     /translation="MNHHRAGQLLSSYLDGELLPGEATAVQEHLLECAACREAFARLR
                     ATKGLLGELAVAEPPVEFWPTVREPQVPRLALSAWRAWRPPVLRPGAAWAAAAAVALA
                     LALALTLFIKGTVDRLHAAEIGVDLYVREHALQMGTEPLADRGFLGVVVGDADLVLVG
                     EPSRPGGSAP"
     gene            complement(936..1559)
                     /locus_tag="VEW91_00120"
     CDS             complement(936..1559)
                     /locus_tag="VEW91_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM90021.1"
                     /translation="MGRPAVVALMAQPGTVRAGSYTPEELAWYETIVQRYARHVYNIA
                     YRMVGNEADARDLSQEAFLRVYRALRRVEPETPLEGWLYRIVSNLSVDLLRKRPRAKV
                     ESLDVPLPTPRGEVAREFPDIAFSPEAILEKEQLDSAIQHALGMLSEDLRLAVVLSDI
                     EGFSYEEIARVMRVPLGTVKSRLHRARQILQQRLRPYVDARRRGWEE"
     gene            complement(1605..>3448)
                     /gene="leuS"
                     /locus_tag="VEW91_00125"
     CDS             complement(1605..>3448)
                     /gene="leuS"
                     /locus_tag="VEW91_00125"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYM90022.1"
                     /translation="YAEELLDFSKMEWPERVQTLQRNWIGRSEGAEIVYTSEQGDPIT
                     VFTTRPDTLFGATFLVLAPEHPLVDKLTTPERRAEVSAYVHRARRQSEIERLAEDREK
                     TGVFIGAYVRHPLTNARVPIYIGDYVLLTYGTGAIHAVPAHDSRDFVFAQKYRLPIPV
                     VIAPPDWDGKPLREAYIGEGTMVNSGPFDGTPSEEGRKRVTEALERAGRGRPAVTYRL
                     RDWLISRQRYWGTPIPIIYCPACGTVPVPYADLPVLLPEDAEFLPTGESPLKLHEGFR
                     RARCPRCGGPAERETDTMDTFVDSSWYQYRYLSPHDPERPFDPERGRKWLPVDQYTGG
                     IEHAVLHLLYTRFFTKAMRDLGLVWLDEPMLRLFNQGIILGSDGYRMSKSRGNVVNPD
                     DYVGALGVDTVRAYLMFIGPWDSGGPWNPKGIEGVHRFLHRVWHLVLDPAPEVPPAAD
                     AGAGTRRAIHHTLKRVTEDLEGFRFNTALAAMMECTNTLMRSREAGAAASADWAPALR
                     TLVLMLAPFAPHAAEELWERMGQPYSVHQQTWPAWDPDAIREETVTLVLQVNGRVRDR
                     VVVPAGLDEASLRETALASEKVRRFIDGMQVQDVIVVRGKLVNVVVR"
BASE COUNT          503 a         1245 c         1177 g          523 t
ORIGIN      
        1 cctcaacccc cagggcggtg acgcggtaat tgcgcaggag gaccgtgaga tcccggccct
       61 ggaactcccc cccgagggtc ggcccctcga acgccatgtt cagacgcggt tcgtagtgcc
      121 aggtgatcgt cccgttgtcc acgatcaagc gcccggccag tgcctcgggg gacaggtact
      181 cgagccgcac cgcatcaggc cgcttgtgcg actcggcgac cgtgatcgtc tcggtccgcc
      241 cgccgcggag cacggtgatg atcttcgtcc cctcgtaatc gaacacgcgg ggcgcgtcga
      301 gcgccatccg cagcaccgct gccggaggca ccaccgggac cggggcgctc aagccgccct
      361 gggctcccac gagcaccagg acggccgcac cagccagcga tcggacgagg gcgggccccg
      421 tcacggcgcc gaccctccgg gacgggaggg ctcgccgacc aggaccaggt ccgcatctcc
      481 gacgaccacc ccgaggaacc cccggtcggc caggggctcg gtgcccatct ggagggcgtg
      541 ctcccggacg tacaggtcca cgccaatctc ggcggcgtgc aggcggtcca ccgttccttt
      601 gatgaagagg gtcagcgcga gggcgagggc cagggcgacc gcggcggcgg ccgcccaggc
      661 cgccccgggc cgcaggacgg gcggccgcca cgcgcgccac gccgagaggg caagccgggg
      721 tacctggggc tcccggacgg tgggccagaa ctcgaccggg ggttcggcca ccgcgagctc
      781 gcccagcagc cccttcgtgg cccggaggcg cgcgaaggcc tccctgcagg ccgcgcactc
      841 cagcaggtgc tcctgcaccg cggtggcctc tccgggcagc agttcgccgt ccaggtacga
      901 cgacaggagc tgccccgcgc ggtgatgatt catcctcatt cctcccaccc ccgccgccgg
      961 gcgtccacgt acgggcgcaa ccgctgctgg aggatctgcc gcgcccggtg aaggcgggac
     1021 ttgaccgtcc ccagggggac gcgcatgacc ctcgcgatct cctcgtacga aaacccttcg
     1081 atgtcgctca acacgacggc gagcctgaga tcctcgctga gcatcccgag cgcgtgctgg
     1141 atcgcgctgt cgagctgctc cttttccaaa atcgcctccg ggctgaacgc gatatcgggg
     1201 aactcccgtg ccacttctcc gcgaggggtc gggagcggga cgtcgagcga ctcgaccttg
     1261 gcccggggcc gctttcgcag caggtcgaca gagaggttgc tgacgatccg gtacagccag
     1321 ccctcgagcg gggtctccgg ctcgacgcgg cgcagcgccc ggtagacgcg cagaaacgct
     1381 tcctgcgaaa ggtccctggc gtccgcctcg ttgcccacca tccggtaggc gatgttgtac
     1441 acgtggcgcg cgtaccgctg gacgatcgtc tcgtaccacg ccagttcctc cggcgtgtac
     1501 gaccccgccc gcacggtgcc aggttgcgcc atgagcgcca ccacggccgg cctgcccatt
     1561 tcccctaccg acgtatcagg ccggcgaaaa gttcctcgtc ttccctagcg gacgacgacg
     1621 ttcaccagtt tcccgcgaac gacgatcacg tcctggacct gcatgccgtc gataaaccgg
     1681 cgaactttct cgctggcaag cgccgtctcc cggaggctcg cctcgtccag gccggccggc
     1741 accaccaccc ggtcgcggac gcgcccgttg acctgcagga cgagggtgac cgtctcctcg
     1801 cggatcgcgt cggggtccca cgccggccag gtctgctggt ggacgctgta cggctgcccc
     1861 atccgttccc agagctcttc ggcggcgtgc ggcgcgaacg gcgccagcat caggaccagc
     1921 gtgcgcaggg ccggcgccca gtcggcgctc gccgcagcgc cggcctcgcg tgaccgcatc
     1981 agggtgttgg tgcactccat catcgccgcg agcgcggtgt tgaaccggaa gccctccagg
     2041 tcctcggtca cccgcttgag ggtgtggtgg atcgcgcggc gcgtccccgc cccggcgtcc
     2101 gccgcgggcg ggacctccgg ggccggatcc agcaccaggt gccacacccg gtggaggaag
     2161 cggtgcaccc cctcgatccc cttcgggttc caggggcccc cgctgtccca gggtccgatg
     2221 aacatcaggt aggcgcggac cgtgtcgacc cccagggccc cgacgtagtc gtcggggttg
     2281 accacgttgc cgcgcgattt gctcatccgg tacccgtcac ttcccaggat gatcccctga
     2341 ttgaacaggc gaagcatggg ctcgtccaac cacaccaacc ccaggtcccg catcgccttg
     2401 gtgaagaacc gggtgtagag caggtgcagc accgcgtgct cgatccctcc ggtgtattga
     2461 tccacgggga gccatttccg gccgcgctcg gggtcgaagg gccgctcggg gtcgtgcggg
     2521 ctcaggtacc ggtactgata ccacgaggaa tcgacgaacg tatccatcgt atcggtctcc
     2581 cgctccgccg ggcctccgca ccgcgggcac cgggcgcgcc ggaagccctc gtgcagcttg
     2641 agcggggatt cgccggtggg caggaactcg gcatcctcgg ggaggagcac cggcaggtcc
     2701 gcgtagggga ccggcaccgt gccgcatgcc gggcagtaga tgatcggaat cggcgtgccc
     2761 cagtaccgct gccggctgat cagccagtcg cgcaggcggt aggtgacggc ggggcggccc
     2821 cggccggcgc gttcgagcgc ctcggtcacg cgtttgcgcc cctcctcgct cggggtcccg
     2881 tcgaacggcc cggagttgac catcgtcccc tcgccgatgt aggcctcgcg gaggggcttc
     2941 ccgtcccagt cgggcggggc gatcaccaca gggatcggca ggcggtactt ttgggcgaag
     3001 acgaagtccc ggctgtcgtg cgccggcacc gcgtggatcg ccccggtccc gtaggtgagc
     3061 aggacgtagt ccccgatgta gatcgggacc cgggcgttgg tgagcggatg gcgcacgtac
     3121 gcgccgatga agacccccgt tttctcccgg tcttcggcga gccgctcgat ctccgattgc
     3181 cgccgcgcgc ggtgcacgta ggcggagact tctgcgcggc gctccggggt ggtgagcttg
     3241 tccaccaggg ggtgctccgg cgcgagcacc aggaaggtgg cgccgaagag cgtgtccggg
     3301 cgcgtcgtaa agacggtgat cggatcgccc tgctcgctcg tgtagacgat ctcggcgccc
     3361 tcgctgcgcc cgatccagtt ccgctggagc gtctggaccc gctcgggcca ctccatcttg
     3421 ctgaagtcca gcagctcctc cgcgtatt
//
LOCUS       DASQGH010000009         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51019, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000009 DASQGH010000000
VERSION     DASQGH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51019"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VEW91_00130"
     CDS             <1..332
                     /locus_tag="VEW91_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYM90023.1"
                     /translation="MATMPAPLAKLGHACYGEGVMRVNVGQAKTDLSRLLARVEAGEE
                     VEIARNGVPVAKLVRIEPQMRPGERFMAIKGALAGQIFTDRDFEFSEEELDEMYAEWD
                     DEDEKFL"
     gene            329..724
                     /locus_tag="VEW91_00135"
     CDS             329..724
                     /locus_tag="VEW91_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYM90024.1"
                     /translation="MRLLLDTHIVLWQLDDRALPAYADEAIEGAAELLFSVVSFMEVG
                     IKAATGKLAARPQLRELVVQGGMRILSLTPEHGLGVSELPLHHRDPFDRLLISQAQAE
                     GLTILTADPYFARYDVPVISALTSDLRED"
     gene            770..1570
                     /locus_tag="VEW91_00140"
     CDS             770..1570
                     /locus_tag="VEW91_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HYM90025.1"
                     /translation="MTQKTLWERTVELTISVGERILAPIERFIGRRSLVGDATFFPLE
                     RFPWVRHVEENWTVIREEVERLLEHHAELPNFQDISKDQIEITDDDRWKTFFLYGYGF
                     EAKLGVEMCPRTAALMRQIPGMTTAMISILSPRKHILDHRGPYKGVLRYHLGLIVPQD
                     AEACRIRVGEDVRHWEEGQSMVFDDTFNHEVWNDTDETRVVLFVDVLRPLPFPESAIN
                     RAIVKAIGLSPFVLDAKRNQEAWERRYLERRRAPAVAVGEPEAQRAAP"
BASE COUNT          284 a          552 c          530 g          254 t
ORIGIN      
        1 cgatggcaac catgcccgcc cccttggcca agcttggtca tgcatgctat ggtgagggcg
       61 tcatgagagt gaatgtcggc caggccaaga ccgacctctc ccgcctgctc gcgcgcgtgg
      121 aggcgggaga agaggtggag atcgcgcgca acggcgtgcc cgtcgccaaa ctggtgcgca
      181 tcgagccgca gatgcgtcct ggcgagcgct tcatggcgat caaaggcgct cttgccggac
      241 agatcttcac cgatcgggac ttcgagttca gcgaggagga actcgacgag atgtatgccg
      301 aatgggacga cgaggacgag aaattcctgt gaggttgttg ctcgacaccc acatcgtcct
      361 ctggcagctc gacgaccgcg ctctgccggc gtatgccgac gaggccatcg aaggcgccgc
      421 cgagctgctc ttcagcgtgg tgtcgtttat ggaggtgggg atcaaggcgg ccaccggcaa
      481 gctcgccgca cggccccagt tgcgggagct cgtcgtccag ggtgggatgc gaatcctcag
      541 cctcacgccc gaacacggcc tcggcgtctc ggagctcccg ctccaccacc gcgacccatt
      601 cgaccggctg ctgatctccc aggcccaggc cgagggcctg acgatcctca ccgccgaccc
      661 gtatttcgcc cgctacgacg tgcccgtgat ctcggcgctc acgtcggacc tgcgcgagga
      721 ctaagctgag cgccgaggag cggcaaagcc agtgaggagg ctgatcgcga tgacgcagaa
      781 gaccctgtgg gagcggacgg tcgagctgac gatctccgtc ggcgaacgga tcctcgcgcc
      841 gatcgagcgg ttcatcggca gacgctcgct ggtcggcgac gcgacgttct tcccgctcga
      901 gcgctttccc tgggtgaggc acgtcgagga gaactggacg gtcatccgcg aggaggtcga
      961 gcggctgctg gagcaccacg ccgagctgcc caacttccag gacatctcca aggatcagat
     1021 cgagatcacc gacgacgacc gttggaagac cttcttcctc tacggctacg gcttcgaggc
     1081 caagctcggc gtcgagatgt gcccgcgcac ggcggccctg atgcgccaga tcccggggat
     1141 gaccaccgcg atgatctcca tcctctcccc ccgcaagcac atcctcgacc accgcggccc
     1201 ctacaagggc gtgctgcgct accacctcgg gctgatcgtc ccgcaggacg ccgaggcctg
     1261 ccgcatccgc gtcggcgagg acgtccgcca ctgggaggag ggccagagca tggtcttcga
     1321 cgacaccttc aaccatgagg tctggaacga caccgacgag acgcgggtgg tcctgttcgt
     1381 cgacgtcctg cgtcccctcc ccttccccga gtcggccatc aacagggcga tcgtcaaggc
     1441 gatcgggctc tcgccgttcg tgctcgacgc caagcgcaac caggaagcct gggaaaggcg
     1501 ctacctggag cgccgccgcg ctccggccgt cgccgtcggc gagccggagg cgcagcgcgc
     1561 agccccttga gtcgctccgc cgtcaggcgg agatcaggtg ggaccgcccc cggggcagat
//
LOCUS       DASQGH010000010         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_25528, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000010 DASQGH010000000
VERSION     DASQGH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25528"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VEW91_00145"
     CDS             <1..709
                     /locus_tag="VEW91_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90026.1"
                     /translation="EIVRARAPIAFGVAVVEDAYDVPVEIAVVAADSFVEREPALLTR
                     ARSLMPSLPVRDIDVLVVQEIGKNISGDGMDPNITGRYLVPHLTGGPTIRRIAVLDLT
                     DATHGNAIGVGLADFIPQRVVDKIDLAPMYMNAITSAITEAVRLPMILAADRDVLWTA
                     VLTTQRPTPGAERLVYIRNTLALKEVAVSTAAIPDLEPQARVASQPFAMKFTGDGALV
                     PPLAPAAVGARPDWGDF"
     gene            713..1519
                     /gene="larE"
                     /locus_tag="VEW91_00150"
     CDS             713..1519
                     /gene="larE"
                     /locus_tag="VEW91_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HYM90027.1"
                     /translation="MESLARKRAHLGEMLRDLGGVLVAFSGGVDSAYLLAATCDALGD
                     RCVGATAVSPSLARDELASARRIAASIGARHLVVETGEYEDPRYRRNDAHRCYFCKHA
                     LFTQLRALAAELDLPVVAYGANVDDLGDWRPGMRAAEEFAVRAPLLDAGLTKSEIRAL
                     ARARGLEVWDKPASPCLASRIPHGTPVTIQALRQIEEGERILRGLGFREVRVRHHGRA
                     ARIEVPPREIPRLQKIVPEIAAVMRTVGFQDVTVDPRGLRSGGQPEEPPR"
     gene            1516..2349
                     /gene="larB"
                     /locus_tag="VEW91_00155"
     CDS             1516..2349
                     /gene="larB"
                     /locus_tag="VEW91_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HYM90028.1"
                     /translation="MNEEALSRLLRDVRSGQTSVDAALAELRWLPFAELGFAKADTHR
                     LLRRRAAEAVYCPGKTVGQVLRIAEELHRSGAVVLLTRAAPEVAAEALRTFPGSSHVA
                     DASLVIVGALPADRSGCVGVLSGGTADVGVAEEAAWTAEAMGAAVTRVYDVGVAGLHR
                     LGAQRELLERAHALVVVAGMDGALPAVVAGLTRAPVIGVPTSVGYGAHLGGLAPLLTM
                     LNACAPGVAVVNVDNGFGAGYLAAVINAGAGAVAPGPPAPRDRVRPDAPPLDSDAHRL
                     P"
     gene            2330..3583
                     /gene="larC"
                     /locus_tag="VEW91_00160"
     CDS             2330..3583
                     /gene="larC"
                     /locus_tag="VEW91_00160"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HYM90029.1"
                     /translation="MRIGYLELGSGASGDMLLAALLGAGWAEEALREVVTRLGVPVRI
                     AVRRVHRRGVPAVRVEVVEDAPPHSRFYPALDRVLAESRIDEPLRRDAMAVLERLAEV
                     EAEVHGTSIEEVALHELGGLDTLVDIVGVLAGFRALRLDRLTASPVNLGRGWVETRHG
                     TVPVPAPATAALVEGMPVYSGEVEGELLTPTGAVLLATLVSDWGALPPLRLEQIGTGA
                     GAADPPRANVLRLFVGEALGPSGTAGPAPAGEASPRERRGVDPASPHTERLVVLETSI
                     DDMTPQLYPHVAAALIAAGALDVAIIPAVMKKGRPGHLLRVLAGPDRAQALCGLLFAE
                     TTTLGVRMHEVTRIAVRRRSVEVETDYGPVPVKIAEDLSGVLNMSPEFEACRALAERH
                     GVPVKQVIAAAQRAAAALEDPRARP"
     gene            3570..>4124
                     /locus_tag="VEW91_00165"
     CDS             3570..>4124
                     /locus_tag="VEW91_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90030.1"
                     /translation="MHVRELFDLTGKAAIVTGGSRGRGFQMASALAEAGAALALTARK
                     ADALEQAAAQLRGRGFRVRAVPGDVSQAEEAEAVVARTLEEFGRVDILINNAGTTWGA
                     PVLDMPLDAWHKVLETNVTGTFLMIQAVGRAMVDAGRGGKIVNLASVAGLVGTDAGVM
                     DAIGYSTSKGAVIALTRDLAVKWAR"
BASE COUNT          524 a         1457 c         1557 g          586 t
ORIGIN      
        1 ggagatcgtg cgtgcgcgcg cgccgatcgc gttcggcgtc gccgtcgtgg aggacgccta
       61 cgacgttccg gtggagattg ccgtggtggc cgcggactcc tttgtggagc gggagcccgc
      121 gctgctcacc cgggcccgga gcctcatgcc cagcctgccc gtccgcgaca tcgatgtgct
      181 cgtggtccag gagattggca agaacatcag cggggacggc atggacccga acatcaccgg
      241 ccggtacctc gtgccgcacc tcacgggcgg ccccacgatc cgcagaatcg ccgtgctcga
      301 cctgaccgat gcgacgcacg gcaacgccat cggggtgggg ctcgccgact tcatccccca
      361 gcgggtggtg gacaagatcg acctcgcgcc gatgtacatg aacgcgatca cctcggcgat
      421 cacggaggcc gtccgcctgc cgatgatcct ggccgccgac cgggacgtgc tctggaccgc
      481 ggtgctgacc acccagcggc ccacccccgg ggcggagcgc ctggtgtata ttcgaaacac
      541 gctcgccctc aaggaggtgg cggtgtccac cgcggcgatc cccgatctcg agccgcaagc
      601 cagggtggcc tcccagccct tcgcgatgaa gttcacgggc gacggcgcgc tcgtgccgcc
      661 tctcgccccc gcggcggtcg gcgcgcggcc ggactggggg gacttctagg cgatggagtc
      721 gctcgcgcgc aagcgcgccc acctcgggga gatgctgcgc gatctcggcg gggtcctcgt
      781 cgccttctcg ggaggcgtcg acagcgccta cctgctcgcc gcaacctgcg acgccctggg
      841 cgaccgttgc gtgggcgcga ccgcggtctc tccctcgctt gcccgggacg agctcgcctc
      901 cgcccgccgc atcgcggcgt cgatcggcgc gcgccacctc gtcgtggaga ccggggagta
      961 cgaggatccc cggtaccgcc ggaacgacgc gcaccgctgt tacttctgca agcacgccct
     1021 gtttacgcag ctgcgggcgc tggccgccga attggatttg ccggtcgtgg catacggcgc
     1081 gaacgtggac gacctggggg actggcggcc cggaatgcgc gccgccgagg agttcgcggt
     1141 acgggcgccg ttgctcgatg ccgggctcac caagtcggag atccgcgcgc tggcgcgggc
     1201 gagggggctc gaggtgtggg acaagcccgc ctcgccgtgc ctcgcctcac gaatcccgca
     1261 cgggacgccg gtcaccatcc aggcgctccg gcagatcgag gaaggagagc gcatcctgcg
     1321 cgggctcggc tttcgcgagg tgcgggtccg ccaccacggc cgggctgcgc ggatcgaggt
     1381 gcccccccgt gagattccgc ggctccagaa gatcgtcccc gagatcgccg ccgtcatgcg
     1441 gacggtgggg tttcaggacg tgaccgtgga tccccgcggg ctgcgctcgg gcgggcagcc
     1501 cgaggagcca ccccggtgaa cgaggaggcg ctctcgcggc tccttcgcga cgtgcggtcg
     1561 gggcaaacga gcgttgacgc agccctcgcg gagcttcgct ggctgccgtt cgccgagctg
     1621 gggttcgcca aggccgatac gcaccgcctc ctccgccggc gggccgccga ggccgtgtac
     1681 tgccccggca agacggtggg tcaggtgctc cgcatcgctg aggagctgca ccgctcgggg
     1741 gcggtggtgt tgctcacccg cgccgctcct gaggtggcgg ccgaggcgct tcgcacgttt
     1801 cccggatcct cccacgtcgc cgacgcatcg ctggtcattg tcggcgcgct cccggcggat
     1861 cgcagtgggt gcgttggggt gctgagcggg gggacggcgg atgtcggcgt ggcggaggaa
     1921 gccgcgtgga ccgccgaggc gatgggggcc gcggtgacgc gggtctacga tgtcggcgtg
     1981 gccggccttc accggctcgg cgcccagcgg gagctgctcg agcgggcgca cgccctggtc
     2041 gtggtggccg ggatggacgg cgcgctcccc gcggtcgtgg ccgggctcac ccgcgcgccg
     2101 gtgatcggcg tgccgaccag cgtcgggtac ggggcgcacc tggggggcct cgcgcccttg
     2161 ttgacgatgc tcaacgcatg cgcgcccggg gtcgccgtcg tcaacgtcga caacggcttc
     2221 ggcgccgggt acctcgccgc ggtcatcaac gccggcgcgg gcgctgtcgc ccccgggccg
     2281 cccgcccccc gcgatcgagt gcggccggac gcgccgcccc tggactcgga tgcgcatcgg
     2341 ctaccttgag ttggggagcg gggcgagcgg cgacatgctg ctcgccgcgc tcctgggcgc
     2401 cggctgggcc gaggaagcgc tgcgcgaggt cgtgacccgg ctcggggtgc ccgtccggat
     2461 cgccgtgcgc cgcgtgcaca ggcggggcgt ccctgcggtg cgggtggagg tggtggaaga
     2521 cgcccccccg cactcgcgct tctatcccgc gctcgaccgc gtgctcgccg agagccgcat
     2581 cgacgaaccc ctccgccgcg acgcgatggc ggtcctggag cgcctcgccg aggtcgaggc
     2641 cgaggtccat ggcacgtcga tcgaagaggt ggccctgcac gaactcgggg gcttggacac
     2701 actggtcgac atcgtcgggg tgctggcggg cttccgggcg ctccggctcg accgtctgac
     2761 cgcctctccg gtgaacctcg gccggggatg ggtcgagacc cgccacggga ccgttccggt
     2821 ccccgcgccg gcgaccgcgg cgctcgtcga agggatgccg gtctactcgg gggaggtcga
     2881 gggcgaactc ctgacaccca cgggggccgt gctgctcgcc acgctggtca gcgactgggg
     2941 ggcgctccca ccgctccggc tcgagcagat cggcacgggc gcgggcgcgg cggatccccc
     3001 gcgcgcgaac gtcctgaggc tctttgtcgg cgaggccctg gggccctccg gcaccgccgg
     3061 tcccgcccct gccggggaag cgtccccccg cgagcgccgc ggggtcgatc ccgcaagccc
     3121 tcacaccgag cggctcgtgg tcctggagac ctcgatcgat gacatgactc cccagctcta
     3181 tccccacgtg gcagccgcgc tcatcgccgc cggtgcgctg gatgtggcga tcatcccggc
     3241 ggtgatgaag aaggggcggc cgggccacct cctgcgcgtc ctggccggcc ccgaccgggc
     3301 gcaggcgctg tgcggactcc tgtttgccga aacgacgacg ctcggcgtgc gtatgcacga
     3361 ggtgaccagg atcgcggtcc ggcgccggag tgtggaggtg gagaccgact acggtcctgt
     3421 gccggtcaag atcgccgagg acctgtcggg cgtcctcaac atgagcccgg agttcgaagc
     3481 ctgccgcgcc ctcgccgagc gccacggggt gccggtgaaa caggtgatcg ccgcggcgca
     3541 gcgggccgcc gccgcgctgg aggatccccg tgcacgtccg tgagttgttc gacctcaccg
     3601 gcaaggcggc gatcgtcacc gggggctcgc gcgggcgggg cttccagatg gcgtcggcgc
     3661 tcgccgaggc gggcgccgcg ctcgcgctca ccgcgcgcaa ggccgacgcc ctcgagcaag
     3721 ccgccgcgca gctgcgcgga cgcggcttcc gggtgcgcgc ggtgccgggc gacgtgagcc
     3781 aggccgagga ggccgaggcg gtggtcgccc gcacgctcga ggagtttggc cgggtcgaca
     3841 tcctcatcaa caacgcgggc acgacgtggg gcgcgccggt cctggacatg ccgctcgacg
     3901 cctggcacaa ggtcctggag acgaacgtca cggggacgtt cctgatgatc caggccgtgg
     3961 gccgggccat ggtcgacgcg ggccggggcg ggaagatcgt caatcttgcc agtgtcgccg
     4021 gacttgtcgg gacggacgcg ggcgtgatgg acgcgatcgg gtacagcacg agcaaggggg
     4081 ccgtgatcgc gctcacccgc gatctggcgg tcaagtgggc gcgg
//
LOCUS       DASQGH010000011         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11379, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000011 DASQGH010000000
VERSION     DASQGH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11379"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1443)
                     /locus_tag="VEW91_00170"
     CDS             complement(142..1443)
                     /locus_tag="VEW91_00170"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HYM90031.1"
                     /translation="MRDASPAPGPDGAGRPAALTSAEIISVGTEHLLGQIVDTNATYL
                     CTVLADLGIAVYFRSTVGDNVRRVQDVFRHALTRADLILSTGGLGPTDDDLTVAAVAE
                     ALGLPLERHEEAWAHVQEFFRKRNRPLSAQQEKQAMLPRGSRMIPNPRGSAPGVIIEH
                     HGKTIIFTPGVPREMRGMVEDTIIPYLRTRGLAGREVIRSRVLRITGLGESAVEDRLR
                     DLMRSSVNPTIAPYAHTGECHVRVTARARDAEAGPVLDRAEAAIRERLGDTVYATGEV
                     KLEEVVARQLVAKRVSIAVAESCTAGLLGHRLTGPPGASAYLDGGLITYSNAAKQRWL
                     GLSGELLERHGAVSAEAARAMAESVREQARTDVGIATTGIAGPTGATPEKPVGLVYIA
                     LAHAGGTEVREVRFGTEPGRAGIKYLASQTALDMLRLHLLR"
     gene            complement(1440..2342)
                     /locus_tag="VEW91_00175"
     CDS             complement(1440..2342)
                     /locus_tag="VEW91_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HYM90032.1"
                     /translation="MAAIGIGERLRNARQALGLSLEEIESTTRIRRAYLEALEREAFG
                     DLPNPAYIRGFLRSYATYLGIPPDELLGLYPGSGAGLTTHPDSAVEVRITPATRFSRT
                     RRVVVGLGIVVGLGVLILGYVLYGQIRQFTQTAPGSQPGSPAPPDAAPGVSPPPGPPG
                     PPGTAPPAPAPGSPAPGPPPPGGPAPPSAPLPKAPAPAPGPTGGGAAPAAEPAAPLTS
                     PIHIVVMASGYSWVRAVVDGTTVFEGFLNAGDKQAWEAAHDLTVKVGNAGAIEVSVNG
                     RSLGRLGGSGQVIERTLSVGSVPR"
     gene            complement(2408..>3280)
                     /locus_tag="VEW91_00180"
     CDS             complement(2408..>3280)
                     /locus_tag="VEW91_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HYM90033.1"
                     /translation="ANYNGIPHLLSPVVTEPREAAAKLRWAIQEMESRYALFAADGVR
                     NIQSFNAQHPDRPLAFIVIIIDELADLMMVAPKDFEEIICRLTQMTRAVGIHLVVATQ
                     RPSVDVITGLIKANIPSRIAFAVTSQVDSRTILDHPGAEKLLGRGDMLFAPIGATRPM
                     RVQGSYISDADSQRLVEFWRTQGRPEYVEELVQAGEEAAAEDGQGDDTLLADAARLVV
                     RAGYGSVSLLQRKMRIGYVRAARLVDQLEEKGIVGPTQGSSPREVRVGLDDLDRILRG
                     GRAHTAQDAAPAEA"
BASE COUNT          480 a         1173 c         1153 g          474 t
ORIGIN      
        1 cgcccagaaa cttgagggta aagtgcagat tgtccggggg aacccaccgg aggccggatg
       61 cggccgagtt caggcgggac cgcaaccccc cgacggcttc gcggagcgcc ggtaccaggg
      121 ccacggcgac gaagatccgc atcaccgcaa caggtgcagc cggagcatat cgagcgccgt
      181 ctgcgaggcg aggtacttga tccccgcgcg accgggttcg gtgccaaagc ggacctcccg
      241 gacctctgtg cctcccgcat gggccagcgc gatgtagacc agtccgacag gtttctccgg
      301 ggttgccccg gtcgggccgg cgatccctgt cgttgcgatc ccgacatccg tgcgggcctg
      361 ctcccggacg gactccgcca tcgcacgcgc ggcctctgcg ctcaccgccc cgtgccgctc
      421 gagcagctcg cccgacagcc ccagccaccg ctgcttggcc gcgttgctgt acgtgatgag
      481 ccccccgtca aggtaggcgg acgcccccgg cgggcccgtc agccggtggc cgagcagccc
      541 ggcggtgcag gattcagcga ccgcgatcga gacccgcttc gccacaagct ggcgggcaac
      601 cacttcctca agcttcacct cgccggtcgc gtagacggtg tcccccaggc gctcccggat
      661 ggcggcctcg gcgcggtcga gcaccggccc cgcctccgcg tcgcgggccc gcgcggtcac
      721 gcggacgtgg cattcgcccg tgtgcgcgta gggcgcgatc gtcggattga cgctgctgcg
      781 catgaggtcc cgcaggcggt cctcgaccgc ggactccccc agccccgtga tccgcagcac
      841 gcgggagcga atcacctcac gccccgcgag ccctcgagtg cgcaggtacg gaatgatcgt
      901 atcctcgacc atcccccgca tctcccgcgg cacgccgggg gtgaagatga tggtcttgcc
      961 gtggtgctcg atgatcaccc caggcgcgct cccgcggggg ttggggatca tccggctccc
     1021 ccggggcagc atcgcctgct tctcctgctg cgcgctcagc gggcggttgc gcttccgaaa
     1081 gaactcctgc acatgggccc aggcctcctc gtggcgctcg agcggaagtc ccagggcctc
     1141 ggccacggcc gcgacggtca ggtcgtcgtc ggtcgggccc aaccctccgg tcgaaaggat
     1201 gagatcggcc cgggtgagcg cgtgccggaa cacatcctgc acccggcgca cgttgtcgcc
     1261 gaccgtggac cggaagtaga ccgcgatgcc gagatcggcg agcaccgtgc acaggtaggt
     1321 ggcgttggtg tccacgatct gcccgagcag gtgctcggtg ccaaccgaga tgatctctgc
     1381 ggaggtcagc gccgccggcc gcccggcccc gtccggaccc ggcgcaggcg aggcgtccct
     1441 caccggggga ccgatcccac ggagagcgtg cgctcgatga cctggccgga gcccccgagc
     1501 cgccccagcg accgcccgtt gaccgagacc tcgatcgcgc cggcgttccc caccttcacc
     1561 gtcaggtcgt gcgcggcctc ccaggcctgc ttgtcgccgg cgttcaggaa tccttcaaag
     1621 acggtggtcc catcgaccac cgcgcgcacc caagagtagc ctgaggccat gacgacgatg
     1681 tggatcggag aggtcaacgg tgcggccggc tcggcagccg gggccgcgcc gccgcccgtg
     1741 ggccctggag ccggggcggg cgctttcggc agaggcgctg agggtggcgc aggacccccc
     1801 ggcgggggcg gaccgggcgc gggcgatccg ggggctgggg ccggtggggc cgtcccgggc
     1861 gggcccggag gcccgggagg cggagaaaca cccggcgcgg cgtccggtgg cgccggtgac
     1921 ccgggttgcg agccaggggc ggtctgcgtg aactgccgaa tctggccgta cagcacgtac
     1981 cccaggatca gcacgcccag gcccacaacg atccccaggc ccacgacgac ccgccgggtc
     2041 cgtgagaacc gggtggccgg cgtgatcctg acctcgaccg cagaatccgg gtgggtggtc
     2101 aggcccgcgc cgctgcccgg gtacagaccg agaagttcgt cgggggggat gccgagatag
     2161 gtggcgtaac tgcgcaggaa tcctcggatg taggcgggat tggggaggtc gccgaaggcc
     2221 tcgcgttcca gcgcctcaag atacgcacgc cgaatccgtg tggtgctttc gatctcttcg
     2281 agcgacaagc cgagcgcctg cctggcgttc cgcaaccgct cgccgatccc aatcgctgcc
     2341 attccccctg gaggttctgg aacgggcgcg ggcattcctg cgaatcccgg gcgagcgccc
     2401 cgacacctca ggcctccgcc ggggccgcgt cctgcgcggt gtgggcgcgc ccgcctcgaa
     2461 gaatacggtc gaggtcgtcg agtcccaccc gaacctcgcg ggggctcgac ccctgcgtcg
     2521 gccccacgat gcccttctcc tcgagctgat cgacgagccg ggcggcccgc acgtacccga
     2581 tgcgcatctt gcgctggagc agggagacgg atccgtaccc cgcgcgcacc acgagccgcg
     2641 ccgcgtcggc cagcagggtg tcatccccct ggccgtcctc cgccgcagcc tcttccccgg
     2701 cctggaccag ctcctcgaca tactcgggcc gcccctgcgt ccgccagaac tccaccagcc
     2761 gctggctatc ggcgtcgctg atgtacgacc cctggacccg catcggccgg gtcgcgccga
     2821 tcggcgcgaa gagcatgtcg ccccgcccca ggagcttctc ggcgcccggg tggtcgagga
     2881 tcgtccggct gtcgacctgg gaggtcaccg caaaggcgat ccgagaggga atgttggcct
     2941 tgatgaggcc cgtgatgaca tcgacggacg gacgctgggt ggcgacgacg aggtggatcc
     3001 ccaccgcccg ggtcatctgg gtgaggcggc agatgatctc ttcgaagtcc ttgggggcga
     3061 ccatcatcag gtcggccagt tcgtcgatga tgatgacgat aaacgcgagg ggccggtcag
     3121 ggtgttgcgc gttgaaggac tggatgttgc gaacgccgtc ggcggcgaac agggcgtagc
     3181 ggctctccat ctcctggatg gcccaccgca gcttggcggc cgcctcgcgg ggctccgtca
     3241 cgacggggct cagcaggtgc gggatgccgt tatagttggc
//
LOCUS       DASQGH010000012         1619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36877, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000012 DASQGH010000000
VERSION     DASQGH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36877"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VEW91_00185"
     CDS             complement(<1..477)
                     /locus_tag="VEW91_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYM90034.1"
                     /translation="MEWGPVFAQALGYVDFLAAHGAPDHQKRWQAVYEKVAFTAEQRE
                     LLAGFVREMHVLCIAGAWCGDCVNQCPIMARIGEQNPKIMVRFVDRDVQPAAQDAAMM
                     NFGRRVPAVIFLSEDDHECGRYGDRTLATYRQMAADRLGPACPTGIVPPGAARMGAV"
     gene            724..>1619
                     /locus_tag="VEW91_00190"
     CDS             724..>1619
                     /locus_tag="VEW91_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYM90035.1"
                     /translation="MKIASVTCTPLAASPPRPVQFSIGTFSTFYAALVEVRTDDGRVG
                     VGECIARRAPEVVTTIVDRLLAPFVIGRDPWDVEGLWDEMLALLRRWGHSRGFVLEAM
                     SGIDIALWDLLARSVGQPLYKFLGGAGRDRVRCYVSKVYFDEIPRMAAEARALAAQGY
                     TAIKVQLGWPEARGGHRADGRTARAVRAAVGPDVEVMLDANGAYDVGTAVRVGRQIEE
                     LNVAWLEEPVPADDLDGYAHRRRSVRVPLAAGETEFGLFGFRDLIACGCVDVLQPEVA
                     RIGGITAARRLGALAHAHNLLYA"
BASE COUNT          223 a          575 c          578 g          243 t
ORIGIN      
        1 caccgcgccc atgcgcgcgg cccccggggg gacgatcccg gtggggcagg ccggccccag
       61 gcgatcggcg gccatctgcc ggtaggtcgc gagcgtgcgg tccccgtagc ggccgcattc
      121 gtggtcgtcc tcgctcaaga agatgaccgc gggcacgcgc cgaccgaagt tcatcatcgc
      181 ggcgtcctgc gcggcgggct ggacatcccg atcgacgaat cggaccatga tcttcgggtt
      241 ctgctcgccg atgcgcgcca tgatcggaca ctggttgacg cagtccccgc accaggcgcc
      301 ggcgatgcac agcacgtgca tctcgcggac gaaccctgcg aggagctcgc gctgctccgc
      361 ggtgaacgcg accttttcgt agaccgcctg ccaccgcttc tgatgatccg gcgcgccgtg
      421 cgccgcgaga aaatcgacgt agccgagcgc ctgcgcaaaa accggtcccc actccatccc
      481 aggtgccccc tctcccgcca tgccctctcc tcgcccaacg cgcccgaccc gaggggaatt
      541 tcccgatgcg aaaccggccg ccatcgtcca cgcgccctac ggtaccaggg tgagcgacgt
      601 ggcgaatgtc gggcgatata atccgaagtc gcgccggtca tatgggtgcg tgaagtgcac
      661 cgcttctgtc aaactcccga gcgccgccga ggagcgcccg gcttcgtccc cgaatacccg
      721 cgcatgaaga tcgcctcggt cacctgcacc ccgctcgcgg ccagcccgcc gcggccggtg
      781 cagttctcga tcggcacctt ctccacgttc tacgccgcgc tcgtggaggt gcggaccgac
      841 gatggccggg tcggggtcgg ggagtgcatc gcccgccgcg cccccgaggt ggtcacgacg
      901 atcgtggacc gcctgctcgc cccgttcgtc atcggccgcg acccgtggga cgtcgaaggg
      961 ctctgggacg agatgctcgc gctgctgcgt cggtgggggc actcccgggg gttcgtcctc
     1021 gaggcgatga gcgggatcga tatcgcgctg tgggacctcc tggcccggtc ggtcggccag
     1081 ccgttgtaca agtttctcgg gggggccggc cgggaccggg tgcgctgcta tgtctccaag
     1141 gtgtacttcg acgagatccc ccggatggcc gcggaggcgc gggcgctggc cgcgcagggg
     1201 tacaccgcga tcaaggtgca gttggggtgg ccggaggcgc ggggcggcca ccgcgcggac
     1261 gggcggacgg cccgggcggt ccgcgcggcg gtcggccccg atgtcgaggt gatgctggac
     1321 gccaacgggg cctacgacgt cggcacggcg gtgcgcgtgg gccgccagat cgaggagctc
     1381 aacgtggcgt ggctcgaaga gccagtcccg gccgacgatc tcgacggata cgcgcaccgg
     1441 cgccgctcgg tgcgggtgcc gctcgccgcg ggcgagaccg agttcgggtt attcgggttc
     1501 cgcgacctca tcgcctgcgg atgcgtggac gtcctccagc ccgaggtggc ccggatcggc
     1561 ggcatcaccg ccgcccgccg gctgggggcg ttggcgcacg cccacaacct gctgtacgc
//
LOCUS       DASQGH010000013         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22709, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000013 DASQGH010000000
VERSION     DASQGH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22709"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..327)
                     /locus_tag="VEW91_00195"
     tRNA            complement(250..327)
                     /locus_tag="VEW91_00195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:Pro,seq:cgg)
     gene            430..1209
                     /locus_tag="VEW91_00200"
     CDS             430..1209
                     /locus_tag="VEW91_00200"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HYM90036.1"
                     /translation="MDELQVAARGAVATLVLNRPAKRNAITLEMWKALPDVLEDLASD
                     PETRVLVIRGAGEEAFAAGADISEFGRVRHDAASGRRYSRTVNQAERALAAFPRPTIA
                     MVRGSCVGGGLEVALACDLRFASRTSRFGITAARLGIVYGYASTRRLATAVGPGHARD
                     LLFSGRLVGAAEALAMGLVTRVCEPEALEPETYEYARRLADQAPLSQKGAKLMLRQLA
                     GEGKMTASDLSACVEQAYESEDYHEGVRAFLEKRPPRFRGR"
     gene            1213..2439
                     /locus_tag="VEW91_00205"
     CDS             1213..2439
                     /locus_tag="VEW91_00205"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYM90037.1"
                     /translation="MRPLDGIRVVDLTRFLAGPYCTQELGDFGADVIKIEARDGDPSR
                     YQTLRPDLEGDSYFFAAANRNKRSVTVDVRTETGREVIRRLVRGADVFVENFRPGVME
                     RLGLGPQTLRAENPRLVYCSITGFGNTGPYSQRPGFDQVGQGMSGFMSITGQEPTGPT
                     RAGIALADLTCAMTACRGILFALLARERTGQGQEVRVAILDSMVALLTWSAGMYFETG
                     APPGVAGNHHPLSAPFGVYHAKDGPFNLAAGNERMWVRLCETLGRPELAHDPRFRTTN
                     DRVAHRAELDAILGETFRTRTRAEWVAYLNERGVASGPIYNLAEVFQDPQVRHQQMLV
                     EMPHPVHGRVKLLGMPIKLSETPGAFRLPPPLLGEHTDEVLREIGYTAEAIAEMRASG
                     VVGALPGSAPRDSSAT"
     gene            2436..3644
                     /locus_tag="VEW91_00210"
     CDS             2436..3644
                     /locus_tag="VEW91_00210"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYM90038.1"
                     /translation="MTPATAPAGPLAGVRVLDLGNMIAGPTCTRVLADLGGDVIKVEQ
                     PGRGDPVRGWRGQYQGHSLLWKLLGRNKRAITLDLHHEEGQRLCRRLYALVDIVVENF
                     RPGVLERWGLAHAQVEEACPHLIMVRISGFGQTGPYRDRAGFGGVAEAMAGVRFLTGY
                     PDRPPTRVGFALADTVAGLYGACAALAALEERRRSGRGQEIDVALTEAMFSLLDDLLP
                     AYQKLGSVAQRHGTGIPGVAPSSIYPTRDGRYIVIGANNDNVFRRLAALMGRTDWLAD
                     PELAGDQGRGRRQAELDAGVASWTREFPLEELTERLAASGVPAGPVYDIAGIYRDPQF
                     RERGMIVELDDPQVGPIALPGVVPRFSRTPGTVAWAGPRMGEHNGEVYGGLLGLLPAE
                     VDDLRARGVI"
BASE COUNT          563 a         1320 c         1357 g          553 t
ORIGIN      
        1 gcggtatagg gagggcatct tcacgtcctt gagctccgag gcactcctct ccttcgagtg
       61 ccgcttactt ctccgcatgc cgccatcttc tgggcttccg atcgcatcca aatcgtatat
      121 cagctaacct tggcacttgt ccaccgggta ctatgaactg gtgtctacct ggtctcattc
      181 cgaaggtccc aacttggtca caaaaaacga ggacacggcc agatacccgt gtccagcgat
      241 gatcttcggt ggtcggggag gagggatttg aaccctcggc ctcagcgtcc cgaacgctgc
      301 gcgctaacca agctgcgcca ctccccgggt ggacccattg tagcattcgc ctccggcgcc
      361 atgcaaccga caggaacggg tgggcccaca ggcgaacgca caacgcctga aggtcccgga
      421 ggaaggtcga tggacgagct ccaggtggcc gcacgcgggg cggtggcgac actcgtgctc
      481 aaccggccgg cgaagcgcaa cgcgatcacc ctggagatgt ggaaggcgct gccggacgtc
      541 ttggaggatt tggcctccga tcccgagact cgggtcctcg tgatccgggg cgcgggagag
      601 gaggcgtttg cggccggcgc cgacatctcc gagttcgggc gggtgcggca tgatgccgcc
      661 tcgggacggc ggtacagccg gacggtgaat caggccgagc gcgcgctcgc ggcgtttccc
      721 cggcccacga tcgcgatggt ccgcggctcc tgcgtcgggg gcgggctcga ggtggcgctc
      781 gcctgcgacc tgcggtttgc cagccggaca tcgcgattcg ggatcaccgc cgcgcgactc
      841 gggatcgtct acgggtacgc cagcacccgc cggttggcga ccgcggtcgg ccccggccac
      901 gcccgggacc tcttgttcag cgggcggctg gtgggggccg cggaagcctt ggccatggga
      961 ttggtgaccc gggtgtgcga gcccgaggcg ctggagcccg agacgtacga gtacgcgcgc
     1021 cggctggcgg atcaggcgcc gctgtcgcag aaaggcgcca agctgatgct gcggcagctc
     1081 gcgggagagg ggaagatgac ggcctcggat ctgtcggcgt gcgtggagca ggcgtacgag
     1141 agcgaggact accacgaagg ggtgcgggcg ttcctcgaga agcggccgcc ccggttccgc
     1201 gggcgatagg cgatgcgccc cctggacggc atccgcgtcg tcgacctgac ccggtttctc
     1261 gccggcccgt actgcaccca ggaactcggc gatttcgggg ccgacgtcat caagatcgag
     1321 gcgcgcgacg gcgatccctc gcgctaccaa acgctccgcc ccgacctcga aggcgacagc
     1381 tacttcttcg ccgccgccaa ccgcaacaag cggagcgtca ccgtggacgt gcgaacggag
     1441 accggcaggg aggtcatccg ccgcctggtc cgcggcgcgg acgtgttcgt ggagaacttc
     1501 cggcccgggg tgatggagcg cctcggcctc ggaccccaga cgctgcgggc cgagaacccc
     1561 cggctcgtct actgcagcat caccgggttc gggaacacgg gcccctacag ccagcgcccg
     1621 gggttcgatc aggtcgggca gggcatgtcg gggttcatga gcatcaccgg acaggaaccc
     1681 accgggccca cccgggccgg catcgccctc gccgacctca cctgtgcgat gaccgcctgc
     1741 cgcggcatcc tgttcgccct cctcgcgcgc gaacgcaccg gccaggggca ggaggtccgg
     1801 gtcgccatcc tcgactccat ggtggccctg ctgacctgga gcgcgggcat gtacttcgag
     1861 accggcgcgc ccccgggggt ggccggcaac caccatccgc tctcggcgcc gttcggggtc
     1921 taccacgcga aggacggccc cttcaacctg gcggccggga acgagcggat gtgggtgcgg
     1981 ctgtgcgaaa ccctcggccg gcccgagctg gcgcacgatc cccggtttcg gaccaccaac
     2041 gaccgggtcg cgcaccgcgc cgagctggac gcgatcctcg gcgagacctt ccggacgcgg
     2101 acgcgcgcgg agtgggtggc ctatctcaac gagcgggggg tggcctccgg cccgatctac
     2161 aacctggccg aggtgttcca ggatccccag gtccgccacc agcagatgct cgtcgagatg
     2221 ccgcacccgg tccacggccg ggtcaagctg ctcgggatgc cgatcaagct ctccgagacc
     2281 cccggcgcgt tccggcttcc gcctccgctg ctcggcgagc acacggatga ggtgctccgc
     2341 gagatcgggt acactgcgga ggcgatcgcg gagatgcgcg cctccggggt cgtgggtgcg
     2401 ctccccggct ccgccccgcg ggacagttcc gccacatgac ccccgccacc gcgccggcgg
     2461 gcccgcttgc gggggtgcgg gtgctcgacc tcggcaacat gatcgccgga cccacgtgca
     2521 cccgggtgct cgccgatctc ggtggcgatg tgatcaaggt ggagcagccg ggccggggcg
     2581 acccggtgcg cggctggcgg ggtcagtacc agggccactc gctcttgtgg aagctcctgg
     2641 gccggaacaa gcgggccatc acgctcgacc tccatcacga ggaaggacag cggctctgcc
     2701 ggcggctcta cgccctcgtc gacatcgtgg tcgagaactt ccggccgggc gtgctggagc
     2761 gctgggggct cgcccacgcc caggtggagg aggcgtgccc gcacctgatc atggtgcgca
     2821 tcagcggctt cggccagacc gggccgtacc gggatcgcgc cgggttcgga ggggtcgccg
     2881 aggcgatggc gggggtgcgc ttcctcaccg gatatcccga ccgccccccg acccgggtgg
     2941 ggttcgcgct cgcggacacg gtcgccgggc tgtacggcgc gtgcgcggcg ctggccgccc
     3001 tggaggagcg ccgccggagc ggccgcgggc aggagatcga cgtcgccctc accgaggcca
     3061 tgttcagcct cctcgacgac ttgctcccgg cgtatcagaa gctcgggagc gtcgcgcagc
     3121 gccacgggac cgggatcccc ggggtcgcgc ccagcagcat ctacccgacc cgcgacggcc
     3181 ggtacatcgt catcggcgcg aataacgaca acgtcttccg ccgcctggcc gccctcatgg
     3241 gcaggaccga ctggctggcg gaccccgagt tggccggcga tcagggacgc ggacgcaggc
     3301 aggccgagct cgacgcgggg gtcgcgtcct ggacccgcga gtttccgctg gaggagctca
     3361 ccgagcgcct cgccgcctcc ggcgtgccgg ccgggccggt ctacgatatc gccgggatct
     3421 accgggatcc ccagttccgc gagcggggga tgatcgtcga actcgacgat ccgcaggtcg
     3481 ggccgatcgc gctgccgggg gtcgtccccc ggttcagccg gacgcccggt accgtcgcgt
     3541 gggccgggcc gcggatgggc gagcacaacg gcgaggtcta cggcggcctc ctggggctcc
     3601 tacccgccga ggtcgacgac cttcgggccc gcggggtgat ctgacagcgc cgcgttgggc
     3661 acacgccgcc ggcgcctgac cctaccaact gttacctctt ggcggagatg aacgtggcgg
     3721 gggtgagcac cgggaccgtg agccgcgcga cgaagtgcga ttgattccac gtgacgaacg
     3781 cctcaaggct cgc
//
LOCUS       DASQGH010000014         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8555, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000014 DASQGH010000000
VERSION     DASQGH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8555"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VEW91_00215"
     CDS             <1..426
                     /locus_tag="VEW91_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HYM90039.1"
                     /translation="ICANFLHEQQNLNASFPSKATNATDYLDQFRMTASYYWNAMYGF
                     TVGFVDTFGSADAKRYPVAPITGSAAGLPDSQALIVQFDWTPWGNDNTHAEFPWLNVR
                     VGLQYTHYLEFNGGTTNYDGSGRNASDNDSYVLFTWFSF"
     gene            521..2074
                     /locus_tag="VEW91_00220"
     CDS             521..2074
                     /locus_tag="VEW91_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90040.1"
                     /translation="MYRYRWGFGLVVLAALAVGALALGPGVLGPRVGAPPSPPATPDQ
                     GSAGTRAAATLSSLVVTPDQGPAGTRIQVAGTGLPPQARVTFLWYTAGGRYRTDVDPG
                     GVVFHGYAYDPMKAVLGDAVADSQGRAVVSFSTPDDLAGPHRISAAVGDREVAQGLFH
                     YLRSATMTPLRGPVGTPIDITIHGLGPAPWNAAALSYDNHYIGFVSAVTTHGTAHVRI
                     RAAGTPGPHYIDLNSASNATPFLNSGQGPGLELLRIVSHRSWTFTVTEGTTFPPTQVE
                     WPAADRVARLGPDAPRTTAPQSQPASGISLALTPTSGLTLSRVEVRAKGLRPGEQAQI
                     VWGTMGRGTRLTTMPSVETQPLLAAAAAPDGALSGQFVVPHDLGGWHTIQLVRDGSVL
                     AYAPYFVARNLVAVTPSRVRAGQQFKVELRGIGWTELDNGVAVTYDNAYAGYACGFNA
                     RGDVIFSLTASGAPGVHLIDLYPMIYQGKGEPPWSYQVPHLTALEDAPGLALGYPLPI
                     FRLAVVVTP"
     gene            2203..2892
                     /locus_tag="VEW91_00225"
     CDS             2203..2892
                     /locus_tag="VEW91_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90041.1"
                     /translation="MGISIAACDAAMATGVAAAVTAPSTDDRPSVRGTIAFAIVEPVQ
                     RGLRVSIIQRLTNATDRALEVTDEDPLVFPLPVVSPPPRGPEPVDFVGGWRKPHVAHG
                     VITDAISMLSGAAEVAYAVGFEPRSRRATLRWVLPYGATDVEVLVADQGIRVSSAGMH
                     AVGMVTERGRRYARWSGGPVRPGEAVSVRLDGLPVSEDRWPEIAAGMLALALAYGLAA
                     ALRRRPAPACL"
     gene            2936..3235
                     /locus_tag="VEW91_00230"
                     /pseudo
     CDS             2936..3235
                     /locus_tag="VEW91_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3322..>3545
                     /locus_tag="VEW91_00235"
     CDS             3322..>3545
                     /locus_tag="VEW91_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM90042.1"
                     /translation="MSREALTAYAFVLPAVVLLLLLVGYPFALSVYLSTSNTVIGGPG
                     RFVGLAQYTRLLHQEIFQQTLLNTAIYAGTA"
BASE COUNT          547 a         1157 c         1202 g          639 t
ORIGIN      
        1 atttgcgcca atttcctcca cgaacagcaa aatctgaatg catccttccc cagcaaagca
       61 accaacgcca ctgactatct tgatcagttc cgcatgaccg cctcctatta ctggaatgcc
      121 atgtacggat tcaccgtggg attcgtcgat acgtttggca gtgcagatgc aaagcggtac
      181 cctgtcgccc cgattacggg cagtgctgcc ggcctccctg attcccaggc cttgatcgtg
      241 caattcgatt ggacgccctg gggcaacgac aacacccacg cggagttccc gtggctcaac
      301 gtgcgggtcg gactgcaata tacgcattat ctggagttta acggcgggac gaccaactac
      361 gacggctccg ggcgcaacgc cagcgacaac gactcgtatg tcttgttcac atggttctcg
      421 ttctaaagtc aggtttcctg acgtgaaggg gctcatgtgc tgggggcgcg ccgcagcggg
      481 ggcgcccccg gccgactgac ggcggcgaag ggagacacag atgtaccgtt atcgatgggg
      541 gttcggcctg gtggtcctgg cggcgttggc cgtcggggcg ctggccctcg ggccgggagt
      601 gctgggacct agggtcggtg ctccgccgtc gcctccggcg acgcccgatc aggggtctgc
      661 cggcacgcgc gctgctgcca cgctgtcgtc gctcgtggtg acgcccgatc aggggcccgc
      721 cggcacgcgc atccaggtcg ccggcacggg cttgccaccc caggcgcggg tgacgttctt
      781 atggtatacc gcgggcggaa ggtatcggac ggatgtggac cccggggggg tcgtcttcca
      841 tggctacgcc tacgacccga tgaaagccgt actcggcgac gcggtggcgg actcgcaggg
      901 gcgggcggtt gtatccttca gcacgcctga cgatctcgcg ggtccgcacc ggatctcggc
      961 ggccgtgggg gaccgtgagg tggcgcaggg gctgttccac taccttcgca gcgccaccat
     1021 gaccccgctg cgcggccccg tgggcacccc catcgacatc acgatccacg ggctgggccc
     1081 ggcgccatgg aacgcggcgg cgctgagcta cgacaaccat tacatcggct tcgtttccgc
     1141 ggtcacaacg catgggacag ctcatgttcg catcagggcg gccggcacgc cgggcccgca
     1201 ctacattgat ctcaacagtg cgtccaacgc gacaccgttt ctcaactcgg gacaggggcc
     1261 gggccttgag cttctccgta tcgtcagcca ccggtcctgg acgtttaccg tgacggaggg
     1321 gaccacgttt cccccgactc aggtggagtg gccggcggcg gaccgggtgg cccgactcgg
     1381 gccggatgct ccgcgaacga cggcgccgca atcccagccc gcgtccggca tctcgctggc
     1441 gctgacaccg acctcggggt tgactctgtc ccgcgtcgag gtccgcgcca agggtctgcg
     1501 gcccggcgag caggcccaga tcgtgtgggg cacgatgggc cggggcaccc gtctgacgac
     1561 gatgccgtct gttgagacgc agcctctttt ggccgccgcc gccgcacccg acggggcact
     1621 cagcgggcag ttcgtcgtgc cccacgacct ggggggatgg catacgatcc agctggtgcg
     1681 ggacggctcg gtgctggcct acgctccgta cttcgtcgcg cggaacttgg tggcggtcac
     1741 cccaagccgc gtcagggccg ggcagcagtt caaggtggag ctcaggggca tcggctggac
     1801 cgagctcgat aacggcgtcg ccgtgaccta cgacaatgcg tacgcggggt atgcctgcgg
     1861 tttcaacgcc cgcggcgatg tcatcttctc cctcacggcg agcggcgcgc cgggggtcca
     1921 cctgatcgat ctctacccga tgatctatca gggtaaaggc gagcctccgt ggtcctatca
     1981 ggtcccgcac ctcacggcgc tggaggatgc ccccggactc gcgctcggct acccgctccc
     2041 gatctttcga ctggcagtcg tggtcactcc atgagacagc catgcgggag tcggaccggc
     2101 gaccgagcgc gtcacaggcg atgaggccgg ccagctgagg acggcgccgt gacgtcacgt
     2161 gcacaaaggg gggccggcgc ggccctcttt ttgtgtgtgg ccatgggcat cagcatagcc
     2221 gcgtgcgacg ccgccatggc gaccggcgtg gccgcggctg tcaccgcgcc ctccaccgac
     2281 gaccggccga gcgtgcgcgg gaccattgcg ttcgcgatcg tcgagcccgt gcagcgcggg
     2341 ctccgcgtga gcatcattca gcgtctgacg aatgcgacgg accgcgccct cgaagtgacg
     2401 gacgaggacc cgctcgtgtt tccactcccg gtggtgtccc cgcccccgcg cggtcccgag
     2461 ccggtcgact tcgtcggcgg gtggcgcaaa ccgcacgttg cgcacggcgt cattacggac
     2521 gcgatctcga tgctgtccgg cgctgcggag gtcgcgtacg cagtcggctt cgagccgcgc
     2581 agcaggaggg cgacgctgcg gtgggtgtta ccgtacggtg ccacggacgt ggaggtgctt
     2641 gtggcagacc aaggcatccg ggtttccagc gccgggatgc atgccgtcgg tatggtaacc
     2701 gaacgcggac ggcgttacgc gcggtggtcc ggtggtcccg tgcgtcccgg ggaggcggtc
     2761 tccgtccgcc tcgacgggtt gccggtgtcc gaggaccgct ggcccgagat cgcggcgggc
     2821 atgctcgcac tcgcgctcgc gtacggcctg gccgcggcgc tgcgccgccg tcccgcgccg
     2881 gcgtgcctgt aattgctcaa tgaaaatgtc acttcatgcc gaaatctggg tgggcaagct
     2941 ggtggcctat ggggaactgt tggtcggggt cgcggtgatc ctcggggcgt tcgtcgggat
     3001 cgcggcgttc ttcggctctc tcatgaactg gaacttcatg atggccggga ccacgagcac
     3061 aaaccccatg ctgttcggcg tagccgtgct cctgatcctc ggctggaagg tcgccgggta
     3121 ctatgggctc gaccggtacc tgcttccggt gctggggacg ccgtggagac ccgggaggat
     3181 cttcgcccat agcgagggca tggcgccggc ctttaccgcg aaagatagaa cgtaatacgc
     3241 aatcggcgaa ctagccgaca gagggcatcc gataccgtgg cagttcaagg cgagcgccgt
     3301 gtcgggcaga ggcagcgcct gttgagccgc gaggcactca cggcatacgc ttttgtgttg
     3361 ccagccgttg tgctcctcct gctgctcgtc gggtatccat ttgcgctctc cgtgtatctg
     3421 tctacaagca ataccgtcat aggcggtccg gggcggttcg tcggcctcgc acagtatacg
     3481 cggttgctcc atcaggagat cttccaacag actcttctca acacggccat ttacgctggc
     3541 acggc
//
LOCUS       DASQGH010000015         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22715, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000015 DASQGH010000000
VERSION     DASQGH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22715"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEW91_00240"
     CDS             complement(<1..447)
                     /locus_tag="VEW91_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYM90043.1"
                     /translation="MQESWHRFLRLGIVHFMAFPAVLRGDGPVVETLRQITGDEFFTA
                     VELGWVNDAAERAAASALLRESHLSVGFGAQSALLVTKSNLNALEEAERKKAVALVQR
                     CIDQAAELGAGRVAVLSGPYPGAADEARAKRSLVESLLDLSAYGRPK"
     gene            complement(508..1323)
                     /locus_tag="VEW91_00245"
     CDS             complement(508..1323)
                     /locus_tag="VEW91_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYM90044.1"
                     /translation="MNLAYVISAAPTRFAAVAQGPDVTQSIRHLAALGYQGVELAIRD
                     PAQVHIDELAEAARGSGVVVPAIGTGQAYLEEGLHLTAPDPAVRERAVARLEAQVPVA
                     RRFGAFLVVGLIHGPIPSTGGRARALEWLLAGLARVARQAATAGVRIVIEPINRYESN
                     WLNTVDEVMDLIGRLGVDNVGVLPDTFHMNIEEQDISGALGRAGPRLWHVHVADSNRR
                     APGAGHLDFRAVVATLSALGYRGAVSAEILQDPSFEAAAAQTIAVMRPLLGHA"
     gene            complement(1345..2307)
                     /locus_tag="VEW91_00250"
     CDS             complement(1345..2307)
                     /locus_tag="VEW91_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90045.1"
                     /translation="MLSTPFVGALLLWTAAKLLRFTRTSYLAALQSVLIAACAYAAVG
                     AIVWRPALARAQDMGSAVVLIALIAIGPALAVCAIAVVKLFGESVGKSAGAVGLSFAT
                     GFVYFVIMIWAVPSLAFTGGVEGSPIDFLRGLLSGSTSQPIDYPVTVIRIVPLGAIPA
                     SDVEAVRQIVGQEFPRITVTVGDPLELPSASFDSARRQYNAEALLGLLAQRAPDRGVR
                     VVGVADVDLFSPGLNFVFSSAQPKGSGVVMSLYDLRDPRESLSRERYRKIVLRALGIT
                     FGFETSLDRSYVMAFSNSLPDLDGKGTAWCGKEPEALKRIQGLR"
     gene            complement(2388..>2625)
                     /locus_tag="VEW91_00255"
     CDS             complement(2388..>2625)
                     /locus_tag="VEW91_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90046.1"
                     /translation="LGLSHLRETLTRHEEFMRQYSARHHFVFFGHAFDQTPLNGEARM
                     TAMKFALVQEVVVILIPNLTAIVYLHRRRAFFRP"
BASE COUNT          457 a          915 c          880 g          373 t
ORIGIN      
        1 cttggggcgg ccgtaggcgg agaggtcgag gagggactcc accaggctcc gcttggcccg
       61 cgcctcgtcc gcggcccccg ggtaggggcc gctcagcacc gccacgcgcc ccgcgccgag
      121 ttctgccgcc tggtcgatgc agcgctgcac cagggccacc gccttcttgc gctccgcctc
      181 ctccagcgcg ttgaggttgg acttggtcac gagcagggcc gactgcgccc cgaacccgac
      241 gctcaggtgg ctctcccgaa ggagcgccga tgcggcggca cgctccgccg catcgttcac
      301 ccagccgagc tcgaccgccg tgaagaactc gtcccccgtg atctgccgga gggtctccac
      361 gaccggcccg tcgccccgga ggaccgcagg gaacgccatg aagtggacga tccccaggcg
      421 gagaaagcgg tgccaggact cctgcatgcg cataacttct cggatccgcg cggccttccc
      481 tgcgcgggcc cctcccggcc gggcccgcta cgcgtggccg agcagggggc gcatcacggc
      541 gatcgtctgc gcggcggcgg cttcgaagct cggatcctgc aggatctcgg ccgacaccgc
      601 gccgcgatac ccgagcgcgg acagcgtcgc gaccacggcg cggaagtcga ggtggccggc
      661 cccgggcgcc cggcggttcg agtccgcgac gtgcacgtgc cagaggcggg gccccgcccg
      721 cccgagcgca ccggagatgt cctgctcctc gatgttcata tggaacgtgt cgggcagcac
      781 ccccacgttg tccaccccga gccggccgat caggtccatc acctcatcca ccgtgttcag
      841 ccagttgctt tcgtaccggt tgatcggctc gatcacgatc cggaccccgg ccgtcgccgc
      901 ctggcgcgcc acccgcgcca ggcctgcgag gagccactcc agcgcgcgcg cgcggccgcc
      961 cgtggagggg atcggcccgt ggatgaggcc cacgaccagg aacgcgccga accggcgggc
     1021 cacgggcacc tgcgcctcga ggcgcgccac ggcccgctcg cgcaccgcgg ggtcgggggc
     1081 ggtgagatgc aggccctcct cgagataggc ctggccggtc ccgatcgcgg gcacgacgac
     1141 gccggacccg cgggccgcct cggcgagctc gtcaatgtgc acctgcgccg gatcgcggat
     1201 cgcaagctcg accccctggt atcccagcgc cgccagatgg cggatcgatt gcgtcacatc
     1261 cggcccctgg gcgacggcgg cgaagcgggt cggggcggcg gagatcacgt aggccagatt
     1321 catccgttaa tcatacactt tggactatcg caggccctgg atcctcttga gtgcttccgg
     1381 ctctttgccg caccacgctg tccctttccc gtcgaggtcc ggcaggctat tcgaaaaggc
     1441 cataacatat gaccggtcaa gggatgtctc gaagccgaag gtgatcccca aagctctcag
     1501 gacgatcttc cggtaccgtt cccggctcag gctttcacgg ggatcgcgga ggtcgtagag
     1561 cgacatgacc acgccgctcc cctttggctg cgcggaggag aacacgaagt tcagccccgg
     1621 actgaacaag tccacgtcgg cgacaccgac gacccgaacc cctcggtccg gggctcgctg
     1681 tgccagcagg cccagcaacg cttcggcgtt gtattgccgc cgcgcggaat cgaaggaggc
     1741 cgagggaagc tccagcgggt ctcccaccgt cactgtgatc cttgggaact cctgcccgac
     1801 gatctggcga accgcctcca cgtccgatgc ggggatcgcc cccagcggga cgatccggat
     1861 cacggtgaca gggtagtcga tcggctggga cgtgcttccg gacaggaggc cgcggaggaa
     1921 atcgatcggt gagccctcga cccctcccgt gaaggcgagg gacgggacgg cccagatcat
     1981 gatgacaaag tacacgaagc cggtcgcgaa cgacaggccg accgcgccgg ccgatttccc
     2041 gacggattcc ccgaagagct tgaccaccgc gatggcgcac acggcgagcg ccggtccgat
     2101 cgcgatgagg gcgatgagaa ccaccgccga ccccatgtcc tgcgcgcggg ccaaggccgg
     2161 ccgccacacg atggcaccga cggccgcgta cgcacacgcc gcgatcaaca cgctttgcag
     2221 ggccgccaga tatgatgtgc gcgtgaatct aaggagcttc gcagcggtcc acaggagcag
     2281 ggcgccgaca aacggggtac tcagcacgaa tagggtgacc ccacccatat agaagaacag
     2341 aaacggttcc agggatgagg aggaacccgg taggggattg agaacggtca cggcctgaag
     2401 aacgcccgcc tgcgatgcag gtagacgatc gcggtgaggt tcgggatcag gatcacgacg
     2461 acttcttgta ccagcgcaaa cttcatggcg gtcatccggg cctcgccgtt gaggggcgtc
     2521 tgatcaaagg catggccgaa gaagacgaag tggtgcctgg ccgagtactg ccgcatgaac
     2581 tcctcgtggc gtgtcagcgt ctcccgcagg tgcgagaggc caagc
//
LOCUS       DASQGH010000016         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8556, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000016 DASQGH010000000
VERSION     DASQGH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8556"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VEW91_00260"
     CDS             complement(<1..1059)
                     /locus_tag="VEW91_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM90047.1"
                     /translation="MKRTSRTAQDLKTLLAVAGGREPADLYLDAATLLNVYSGELYPA
                     NVAVKGRRIAYVGRGHSMVGPQTQVLPLSGRILAPGYVEPHTHITGMATPEEFAREVL
                     RTGTTTLVADTLQILLHTPPDRIPRLLALLGDMPVLMVWFLRLHGASHLPDESMFARD
                     RIHRLLEIEAVRAVGEVTHWPAVYYGDEDLLQKIAMGLAAGRRVEGHAPGTSYERLTA
                     LGAAGWSSDHEAITPEEVIHRLRAGVYTMLRHSSLRPDLPLLAAAITEERARSGRLML
                     TADGPEAVTIVEQGYLSHVIRQAIGAGVPPVLAYQMATINPATYFGLDEEIGGIGPGR
                     RADIVVLNDLRDPTPECVV"
     gene            complement(1098..1355)
                     /locus_tag="VEW91_00265"
     CDS             complement(1098..1355)
                     /locus_tag="VEW91_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90048.1"
                     /translation="MAEPAALEGVSNGELVGRGTWNLSAAGPVTTVRNIWEVRTTKAW
                     MNLLAPLARPIFAWNHAHSMRQGGEGLARLLGARLVESTPG"
     gene            1376..2191
                     /locus_tag="VEW91_00270"
     CDS             1376..2191
                     /locus_tag="VEW91_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HYM90049.1"
                     /translation="MAGEPPEIVESRAGAGIVLAWVSPRKAGRLEVRMPKATWTPLLA
                     EAVGTFTLVFAGAGSIIVTEASKRGAGLLGIALVQGGDGLLGIALAHGLAIAVMVSAF
                     GAISGGHFNPAVTCGFLASGRMQPGQGLRYIAAQLAGATLGAVALRIGFPAGPAAAVH
                     LGTPHLGAGVGFGAGAFLEAIGTFFLVLVVFGTAVHPQAPRIGGLAIGLTITMDILAF
                     GPLTGAAVNPARAFGPALLGNAWDAHLVYWIGPIVGGAAAGWLYSAAYLREVR"
     gene            complement(2170..3648)
                     /locus_tag="VEW91_00275"
     CDS             complement(2170..3648)
                     /locus_tag="VEW91_00275"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYM90050.1"
                     /translation="MRLVLADTPPELCACDLLALPIPEPGTLGGEAADVDRAVGGRLA
                     EIARAEGFSGKPNRTVLLQCPDGPARRILLVGVGRDPTPERFRRYGAIAIRRARDLRA
                     ARAALTTSTGGTGYSADEYVRGVAEGAGLGAYRFERYRKAENGAEVVEAVVISRRGDR
                     TALERAMAAADAAVRATCRARDLVNEPSNVLTPTALAEVARDVAASGGLECRVIDLEE
                     ARAMGMGLFAAVAQGSAQPPKFIVLEYRPEGARRTIGLVGKGITFDSGGLSIKTADGM
                     KTMKGDMAGAAAVIATMGALRELGVPDRVLGIAAATENMISGAATRPGDIVRGLNGAT
                     VEITNTDAEGRLVLADALAFAIRAGAEEVIDVATLTGACVVALGDWTAGVMGNHQPLV
                     DGLLAAAAQAGEQVWQLPLYEEFLEAMRGEITDLRNSAGREGGAERAAAFLGEFVGTT
                     PWAHLDIAGPAFAESAPPAPYLPTGGTGFGVRTLLRYLTSRK"
     gene            complement(3739..4701)
                     /locus_tag="VEW91_00280"
     CDS             complement(3739..4701)
                     /locus_tag="VEW91_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HYM90051.1"
                     /translation="MRARRSRHAPPRLLLSFVLGMVLGAGLATSLVLALRDHPVRPRP
                     AAPSAAHPPATRGAPGGVPAPAPRQASPQPAQEPPRQNPELSRGPAIVPGPSPARSGR
                     PRVAIIFDDAGYGIRGAREVEAIGRPVTISILPHLPYSTLIAEEAPARGIQVILHLPL
                     EPDDETIRLGEGGVRVAMSDQEIRQTIAADLTTVPTAVGANNHMGSLGTSDPRVMRAV
                     MEEMKARHLFFVDSVTSPHSVAAQIARETGVPTAARAVFLDNRDDEEYVRGQFRVLIA
                     LAQARGQAIAIGHVGRVTAGVLVSLLPEFDEAGIQLVRASDLVR"
     gene            complement(4744..>5350)
                     /locus_tag="VEW91_00285"
     CDS             complement(4744..>5350)
                     /locus_tag="VEW91_00285"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYM90052.1"
                     /translation="GAAVGGGPGWPAATGPHGPVVVAVDAGHGGSDPGAIGPTGVKEK
                     DVVLAIAQALRRLLAHQHVDVVMVRDADVFVPLEDRAQIASRGGATLYVSIHANAATD
                     PNASGTQTFYSNPMSQPFAASVLEEVGRAAGLASRGTNQASFKVLVDTSRIPSILVET
                     AFITNPREEQLLRDPSVQQGMAQGILKGIQRYLSVQQSAAP"
BASE COUNT          741 a         1951 c         1860 g          798 t
ORIGIN      
        1 caccacgcac tcgggcgtcg ggtcgcgcag gtcgttcagg accacaatgt ccgcccgccg
       61 cccgggcccg atcccgccga tctcctcatc gagcccgaag tacgtggcgg ggttgatcgt
      121 cgccatctga taggcgagca ccggtggcac tcccgccccg atcgcctgcc ggatcacgtg
      181 gctgaggtac ccctgctcca cgatcgtcac cgcctccgga ccgtctgcgg tgagcatcag
      241 gcgccccgac cgcgcgcgct cctcggtgat cgccgccgcg aggagcggga ggtccggccg
      301 gagcgacgaa tggcgcagca tcgtgtacac gcccgcgcgg agccggtgga tcacctcctc
      361 gggtgtgatc gcctcgtggt ccgaagacca gccggcggcc cccagcgccg taagccgctc
      421 atacgatgtc ccgggcgcat gcccctcgac gcggcggccc gcggccagcc ccatggcgat
      481 cttctgcagc agatcctcgt cgccgtagta gacggccggc cagtgcgtaa cctctccgac
      541 ggcccgcacc gcctcgatct ccagcaggcg gtggatccgg tccctcgcga acatcgattc
      601 gtccggcagg tgggatgcgc cgtgcagccg gaggaaccag accatcagca ccggcatgtc
      661 gccgagcagc gcgaggaggc gggggatccg gtccgggggg gtgtgcagca ggatctgcag
      721 cgtatcggcc acgagtgtcg tggtgccggt ccgcagcacc tcgcgggcga actcctccgg
      781 ggtggccatg ccggtgatgt gggtgtgggg ctcgacgtag cccggcgcga ggatcctacc
      841 cgacagcggc agcacctggg tctgtggccc gaccatgctg tggccgcggc caacgtacgc
      901 gatccggcgc cccttcaccg caacgttggc cggatagagc tcgccgctgt acacgttcag
      961 cagcgtggcg gcatccaggt acaggtcggc cggctcgcgg ccgccggcca ccgccagcag
     1021 cgtcttgaga tcctgagccg tccgggaggt gcgcttcacg atcgagggac cagagaagcc
     1081 gcgcgggcgc ggcgccctca gcccggcgtg ctctcgacca ggcgggcgcc gagcagcctg
     1141 gcgagtcctt cgccgccctg gcgcatcgag tgcgcgtggt tccaggcgaa gatggggcgg
     1201 gcgagcggag cgagcaaatt catccaggcc ttcgtcgtcc gcacctccca gatgtttctg
     1261 acggtcgtca ccggccccgc cgcggaaagg ttccaggtgc cccgccccac cagctcgccg
     1321 ttgctgacgc cctcgagcgc ggccggctcc gccacatcta ccctccttcg atcccgtggc
     1381 gggagaaccc cccgagatcg tcgaatcccg cgccggggcc gggatcgtgc tcgcgtgggt
     1441 ctcgccccgc aaggcgggcc gtctggaggt gcgcatgccg aaggccacgt ggaccccgct
     1501 cctcgccgag gcggtgggaa ccttcaccct cgtctttgcg ggcgcaggct cgatcatcgt
     1561 caccgaggcc tccaaacgag gggccggtct tctcgggatt gcgctggtgc aaggagggga
     1621 cggcctcctc gggattgcgc tggcgcacgg gctggcgatc gcggtgatgg tctccgcgtt
     1681 cggggccatc tccggcgggc acttcaaccc ggcggtgacc tgcgggttcc tggcctccgg
     1741 tcgaatgcaa ccaggacagg gactgcgcta catcgccgcg caactggccg gcgccaccct
     1801 gggcgccgtg gcgctccgaa tcggcttccc cgcgggcccg gccgccgccg tccacctggg
     1861 gactccccac ctcggcgccg gcgtgggatt cggggcgggg gcattcctcg aggccatcgg
     1921 gaccttcttc ctcgtcctgg tggtgtttgg caccgccgtg caccctcagg cgccccgaat
     1981 cggcggcctc gcgatcgggc tgacgatcac catggacatc ctcgcctttg gaccgctgac
     2041 cggtgccgcg gtgaacccgg cgcgcgcgtt cggaccggcg ctcctcggta atgcgtggga
     2101 cgcgcatctg gtgtactgga tcgggccgat cgtgggcggc gctgcggccg ggtggctcta
     2161 ctcggccgcc tacttgcgcg aggtcaggta gcgcagcagc gtccggacgc cgaaccccgt
     2221 gccgcctgtc ggcagatagg gggccggcgg ggcgctctcg gcaaacgccg ggccggcaat
     2281 gtcgaggtgc gcccaggggg tcgttcccac gaactcgccg agaaacgccg ccgcccgctc
     2341 cgcgccgcct tcgcggccgg cggaattccg gaggtcggtg atctccccgc gcatcgcctc
     2401 aagaaactcc tcgtagagcg ggagctgcca cacctgctcc ccggcctggg cggccgccgc
     2461 gaggagcccg tccacgaggg gctgatggtt tcccatcacc ccggccgtcc agtccccgag
     2521 cgccacgacg caggccccgg tcaacgtggc gacgtcgatg acctcctcgg cccccgcgcg
     2581 gatggcaaac gcgagcgcgt cggcgagcac cagccgccct tcggcgtcgg tgttggtgat
     2641 ctctaccgtg gcgccgttga gaccgcgcac gatgtccccg ggccgcgtcg cggcgccgct
     2701 gatcatgttc tcggtggccg ccgcgatccc gaggacccgg tccggcaccc cgagctcccg
     2761 gagggccccc atcgtggcga tcaccgccgc cgcgccggcc atgtcgccct tcatcgtctt
     2821 catcccgtct gccgtcttga tcgagaggcc gccgctgtcg aacgtgatgc ctttgcccac
     2881 gagcccgatg gtccgcctgg cgccctcggg ccggtactcc agcacgatga acttcggggg
     2941 ctgtgcgctg ccctgggcca cggcggcgaa gagccccatc cccatcgccc gcgcctcctc
     3001 gagatcgatg acccggcact cgagcccccc agaggcagcg acgtccctgg cgacctcggc
     3061 cagcgcggtg ggcgtgagca cgttggacgg ttcattgacg aggtcgcgcg cgcggcaggt
     3121 cgcgcgcacc gccgcatcgg cggccgccat cgcccgctca agcgccgtgc ggtctccccg
     3181 gcgggagatc accacggcct cgaccacttc cgcgccgttc tccgccttgc ggtaccgctc
     3241 gaatctgtag gcgccgagcc ccgctccctc cgccaccccg cggacgtact cgtcggcgga
     3301 gtagcccgtg ccccccgtgc tcgtggtcaa cgccgcacgg gctgcgcgga ggtcgcgcgc
     3361 cctccggatc gcgatcgccc catagcggcg gaagcgctcg ggagtaggat cccgtccgac
     3421 gcccaccagc aggatccgcc gcgccggtcc gtccgggcac tggaggagca cggtgcggtt
     3481 gggctttccc gaaaagcctt cggctcgcgc aatctccgcc agccgtccgc ccaccgcccg
     3541 gtccacatcc gcggcctccc cgccaagggt ccctggttcg gggataggca gcgcaagcag
     3601 gtcacaggcg cacagttcgg gcggtgtgtc ggcgagcaca aggcgcatcg aggtcctcct
     3661 ggggcgcgcc cccggcgggg cgcccgccgc gtgagattgc gtcagattga ttgtagcagg
     3721 aaacttccgc cgagaacgtt agcggacgag gtcggaggcc cggacgagct gaatcccggc
     3781 ttcgtcgaac tccggtagca gggacacgag cacgccggcc gtgaccctgc ccacatgccc
     3841 gatggcgatc gcctgccccc gcgcctgggc gagggcgatg aggacccgga actgtccccg
     3901 cacgtactcc tcatcgtccc ggttgtcgag gaacaccgcc cgcgcggcag tgggcacgcc
     3961 cgtctcccgg gcgatctggg cagcgaccga gtggggagag gttacgctgt ccacaaagaa
     4021 gagatgccgc gccttcatct cctccatcac ggcgcgcatc acccgggggt cggaagtgcc
     4081 gagcgacccc atgtgattgt tcgcgccgac tgcggtgggc accgtggtga gatccgccgc
     4141 gatcgtttgt cggatctcct ggtcgctcat cgcgaccctg acacctcctt cgccgagccg
     4201 gatcgtctcg tcgtcgggtt ccagggggag gtgcaggatg acctgaatcc cgcgcgcggg
     4261 ggcttcctca gcgatcaggg tcgagtaggg aaggtggggg aggatcgaaa tcgtcaccgg
     4321 ccggccgatc gcttcgacct ccctcgcgcc acggatgccg tatcccgcat catcaaagat
     4381 gatcgccacg cgggggcggc cggatcgcgc aggcgaaggg ccggggacga tggcgggccc
     4441 ccgggacagc tcagggttct ggcgcggcgg ttcttgagcc ggctgcggag acgcctgccg
     4501 gggagccgga gcgggcaccc ccccgggcgc gccacgggtg gccggggggt gggcggcgga
     4561 aggggccgcg gggcgtgggc gcaccgggtg gtcgcgcagg gccagcacaa gactggtcgc
     4621 gagccccgcg cccagcacca tcccgaggac gaagctgagc aggagccgcg ggggcgcgtg
     4681 cctcgaccgt cgtgcgcgca ttcaaccgcc tcccaatagc gccgccgtgt ggctatccgg
     4741 cgcctacggc gccgccgatt gctgcacgga cagatatcgc tggattccct tcaggatacc
     4801 ctgggccatc ccctgctgga ccgaaggatc gcggagcaac tgttcttccc gcggattggt
     4861 gatgaacgcg gtctccacca ggatcgacgg gatccggctg gtgtcgacca gcaccttgaa
     4921 cgacgcctgg ttggttcctc gcgaggccag cccggccgcc cggccgactt cctcaaggac
     4981 cgaggcggcg aagggctggc tcatcgggtt cgaataaaag gtctgggtcc cgctcgcgtt
     5041 ggggtcggtc gcggcgttgg catgaatgct cacgtacagc gtggccccgc cgcgggacgc
     5101 gatctgcgcc cgatcctcga gggggacgaa cacatcggcg tcgcggacca tgacgacatc
     5161 gacgtgttga tgggcgagca agcgcctgag cgcctgggcg atcgccagga cgacgtcctt
     5221 ctctttgaca cccgtcgggc cgatggcccc ggggtcgctc cccccgtgac ccgcatcgac
     5281 cgctacgacg accggcccat gcggccccgt ggctgccggc catcccggtc ctcccccgac
     5341 cgcggcgccc
//
LOCUS       DASQGH010000017         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42561, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000017 DASQGH010000000
VERSION     DASQGH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42561"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..725)
                     /locus_tag="VEW91_00290"
     CDS             complement(57..725)
                     /locus_tag="VEW91_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HYM90053.1"
                     /translation="MFGLEFWARWLGIVIIDLTLAGDNALAIALAVRTLPPGQRTWGR
                     ILGSLGGILLRLAFIAALTVLLATPLLQLLAGMLLVWIALRLVREDPGAQAHARPGMT
                     LLEAIWLIVLADVGMSLDNILAIAAVAGGNYVLVIFGLSLSLPLVIWGSGIVAQWMNR
                     RAWIIWLGGGVLGYAAGEMMLKDPVLQAWLGRAILGALHYAFPVALGLIVAARGWWRA
                     RGIP"
     gene            complement(826..1410)
                     /gene="msrA"
                     /locus_tag="VEW91_00295"
     CDS             complement(826..1410)
                     /gene="msrA"
                     /locus_tag="VEW91_00295"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYM90054.1"
                     /translation="MSDVRPITPPPGREVATLAGGCFWCLEAVFDDLRGVERVESGYS
                     GGAVPHPTYRQVCTGATGHAEVVQITFDPRVLSFKDLLEVFFAVHDPTTLNQQGSDVG
                     TQYRSAIFYHTAEQRATAEQVIKELEATRVWPAPIVTEVAPFTAFYQAEDYHQEYFRL
                     NGQQPYCRVVIAPKVAKFRKLFGEKLKREQNVAR"
     gene            1552..>1822
                     /locus_tag="VEW91_00300"
     CDS             1552..>1822
                     /locus_tag="VEW91_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M19"
                     /protein_id="HYM90055.1"
                     /translation="MLIADGHLDLAMNGLQWNRDLLSSVYTIRAQEAHTPGKGRGQNT
                     VALPEMRRGRIAVSVVTLIARSTGVPAPHLDFATQIQAHGIARGQL"
BASE COUNT          334 a          626 c          594 g          268 t
ORIGIN      
        1 gccctcctcc tcggagcccc gcgtgggacc gcatcgctcc ccgattcccg cgggggctag
       61 ggaatccctc tcgcgcgcca ccagccgcgc gccgccacga tcagtcccag ggccaccgga
      121 aaggcatagt gcagggcccc caggatcgcc cttcccagcc acgcctggag gaccgggtcc
      181 ttcagcatca tctcgccggc cgcatagccg aggacccctc cgcccagcca gatgatccac
      241 gcgcgccggt tcatccattg ggccacaatg ccgctgcccc agatgaccag gggcaacgac
      301 agcgagaggc cgaagatgac cagaacgtag ttgcccccgg cgaccgcagc gatggcaagg
      361 atgttgtcca ggctcatccc cacatcggcc aggacgatca accagatggc ctccagcagc
      421 gtcatccccg gccgggcatg cgcttgggct ccgggatctt cgcggacgag cctcagcgca
      481 atccacacca gcagcatccc ggcgagcaac tgcaacagcg gagtggccag gaggaccgtc
      541 aacgcggcga tgaaagccaa ccgcaggagg atgcccccga ggcttccgag gatccgtccc
      601 cacgtccgct ggccgggggg caaggtccgc accgccagcg cgatggcgag cgcattatcg
      661 ccggccaggg tcaggtcgat gatgacgatc ccgagccagc gcgcccagaa ttcgagccca
      721 aacaacgacg gcgtcccgga gggcacctgg atcagcgtct gcccccgccg ccgcgttcag
      781 tccggcgttg cgtggcggcg ggggcgctgt gcggggggcg ggctctcaac gggcgacgtt
      841 ctgctccctc ttgagcttct cgccaaacag cttccggaat tttgcgacct tgggggcgat
      901 cacgacacgg cagtagggct gctgcccatt caatcggaaa tactcctggt gatagtcctc
      961 ggcctggtaa aaggcggtga acggtgcgac ctccgtgacg atcggggcgg gccagaccct
     1021 cgtcgcctcc agctctttga tcacctgctc ggcggtcgcc cgctgctcgg cggtgtggta
     1081 gaagatcgcc gagcgatact gcgtaccaac atcggagccc tgttggttga gggtggtcgg
     1141 atcgtgcaca gcaaaaaaca cctccaagag atccttgaac gagagcaccc gggggtcgaa
     1201 ggtgatttgc accacctccg catgcccggt ggcgcctgtg cagacctgac ggtaggtggg
     1261 atggggcacc gcccccccgg agtacccgga ttccacgcgc tccacgcccc tcaggtcgtc
     1321 aaagaccgct tccaggcacc agaagcagcc gccggcgagc gtggccacct cgcgccccgg
     1381 tgggggggtg ataggacgca catcgctcat gggcgatccc tccgccatac cctacgacca
     1441 ggttcgtctt ttaagatagc acatcccgtg gcgcggggag ggggagcaac gggggtcccc
     1501 gtcccccgcc gcgaacattg ctgacacatc ctaaggacgg aggggtgcgc ggtgctcatc
     1561 gcggacggcc atctcgatct cgcaatgaac gggctgcagt ggaaccggga tctcctgtcg
     1621 tcggtgtaca cgattcgggc gcaagaggcg catacgcccg gcaaagggag gggccagaac
     1681 acggtcgcgc tgccggagat gcggagaggc cggatcgccg tgagcgttgt gacgctgatc
     1741 gcgcgctcaa ccggcgtgcc ggcaccgcac ctcgatttcg ccacccagat ccaggcgcac
     1801 gggatcgcca ggggacaact gg
//
LOCUS       DASQGH010000018         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45405, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000018 DASQGH010000000
VERSION     DASQGH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45405"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VEW91_00305"
     CDS             <1..355
                     /locus_tag="VEW91_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM90056.1"
                     /translation="QGFLAKNLPYGEQRRLEIARALATKPQLLLLDEPAAGMNPRESL
                     ETMAFIRRLRDELGITILLIEHQMRVVMGVSERVTVLDYGMKIAEGTPREIQNDPRVI
                     EAYLGTRRAAAGPAG"
     gene            371..1081
                     /locus_tag="VEW91_00310"
     CDS             371..1081
                     /locus_tag="VEW91_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90057.1"
                     /translation="MALLDIRDLHVYYGAIHALRGLSLEVETGKIVALIGANGAGKST
                     ALNTISGLLRPAQGSVRLEGEDLAVVPPHEIVLKGVVQVPEGRRIFGRLTVLENLEMG
                     AFTRTDQRDIQEGLDRTFALFPRLKERATQVAGTLSGGEQQMLAIGRALMARPRVLLM
                     DEPSMGLAPILVEQIFDAIKDINLGGTTILLVEQNAYMALTIAHRGYVLQTGSIVLSG
                     AAAELQANEDVKRAYLGE"
     gene            1115..>2603
                     /locus_tag="VEW91_00315"
     CDS             1115..>2603
                     /locus_tag="VEW91_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYM90058.1"
                     /translation="MSVAGTRGRGPATRLTGGQAAVQCLRVQGIDVVFGLPGVQLDWL
                     FDALHQEREAITVYHTRHEQAVSYMADGYARTAGRVGTCLMVPGPGLLNAMAGLATAY
                     ACSSPVLCLTGQIHSTEIGAGRGALHEIRDQLGAVRSVTKWAARAARPEDIPGVFRDA
                     FRQLLTGRARPVAVEIPPDVLQSPAEIPPAERVTVERQVGDPEVIERAAQALGASQRP
                     IIFAGGGVLRSGAWEPLRQLAEMLEAPVVLSPSGKGALSDRHPLAQGMLAAQDLLPGS
                     DAVLIVGTRFLQPAKSAWGPRAGQTLIQIDIDPEEIGRNCPVAIEIVADAGWALETLA
                     GRLPRHNRRRGSRRDELAAAKRGAQDRLLALQPQAAYAAAIREALPDDGILVSEITQV
                     GYWASYGFPVYQPRTFITAGYQGTLGYGFATALGAQVAAPGRKVVSINGDGGFMYNVQ
                     ELSTMVRHRLPVVVVVFNDNAYGNVRRIQRESFGGRLIASDLHNPD"
BASE COUNT          394 a          860 c          960 g          389 t
ORIGIN      
        1 gcaggggttt ctggccaaga acctgccgta cggcgagcag cgcaggctcg agatcgcgcg
       61 cgcgctggcg accaaaccgc agctgctgct cctcgatgag ccggccgcgg ggatgaaccc
      121 ccgggagagc ctcgagacga tggcattcat ccgccgcctc cgtgatgaac tggggatcac
      181 gatcctcctg atcgagcacc agatgcgcgt ggtgatgggt gtgtccgagc gcgtcaccgt
      241 cctggactac ggcatgaaga tcgcggaagg gaccccccgg gagattcaaa acgacccgcg
      301 ggtgatcgag gcgtacctgg gcacgcgtcg ggcggcggcg ggcccggccg gctaggggag
      361 gggccccacg atggcgctcc tggacatccg cgatctccac gtgtactacg gggccatcca
      421 cgcgcttcgg ggcctctcgc tcgaggtcga gaccggcaag atcgtcgcgc tgatcggcgc
      481 gaacggcgcg gggaagagca cggccctaaa cacgatcagc ggccttttgc gccctgccca
      541 ggggtccgtc cggctggagg gggaggatct cgcggtcgtc ccgccccatg agatcgtgct
      601 caagggcgtc gtgcaggtgc cggaagggcg gcggatcttc ggacgcctga cggtcctcga
      661 gaatctcgag atgggagcct ttacccggac cgaccagcgg gacatccaag aggggctgga
      721 ccggacgttc gcgctctttc cccgcctgaa ggagcgggcg acccaggtgg ccggcacgct
      781 gtccggcggg gaacagcaga tgctggccat tggccgcgcg ctcatggccc ggccgcgggt
      841 cctgctgatg gatgagccgt ccatggggtt ggcgccgatc ctggtcgagc agatctttga
      901 cgccatcaag gacatcaacc tcggggggac gaccattctc ctcgtcgagc agaatgccta
      961 catggcgctc accatcgccc accgcggcta cgtcctccag accggcagca tcgtgctgag
     1021 cggcgcggcg gcagagcttc aggccaacga agacgtcaag cgggcctacc tgggggagta
     1081 gcagcccggg ggaataactc aggaggtgga tcgggtgtcg gtagcgggta cgcggggacg
     1141 cggtccggcg acgcgattga cgggaggcca ggccgccgtc cagtgcctga gagtccaggg
     1201 catcgatgtg gtgtttggcc tgcccggggt ccagctggac tggctgttcg acgcgctgca
     1261 tcaggagcgc gaggcgatca cggtctacca tacgcgccac gagcaggccg tgtcctacat
     1321 ggccgacggg tacgcccgca cggccggccg ggtggggacc tgcctcatgg tgcccgggcc
     1381 gggcctgctg aacgcgatgg cggggctcgc gaccgcgtac gcctgctcgt cgccggtcct
     1441 ctgcctgacc ggccagatcc actcgacgga gatcggggcc ggccggggcg ccctgcacga
     1501 gatccgggac caactggggg cggtgcgctc ggtgaccaag tgggccgccc gcgcggcgcg
     1561 gcccgaagac atccccgggg tgttccgcga cgcgttccgg cagttgctca caggccgcgc
     1621 ccggccggtg gcggtcgaga tccctccgga cgtactccaa tcgccggcgg agatcccgcc
     1681 ggccgagcgc gtgacggtgg agcggcaggt cggggatccc gaggtgatcg agcgggcggc
     1741 tcaggcgctg ggcgcctccc agcgcccgat catctttgcg gggggcgggg tgctccggtc
     1801 gggcgcgtgg gagccgctcc ggcaactggc ggagatgctc gaggcgccgg tcgttctctc
     1861 cccgagcggc aagggcgcgc tgtccgaccg gcaccctctg gcgcagggca tgctcgccgc
     1921 gcaggatctg ctgcccggct ccgatgcggt cctgatcgtc gggacgcgct ttctccagcc
     1981 ggcgaagtct gcctggggcc cgcgtgccgg ccagaccctg attcagatcg acatcgatcc
     2041 cgaggagatc gggcggaact gcccggtcgc gatcgagatc gtcgccgacg ccgggtgggc
     2101 gctcgagacg ctcgccggcc ggctcccccg gcacaatcgc cgccggggct cgcgccgcga
     2161 cgaactggcg gcggccaagc ggggagcaca ggaccggctc ttggcgttac agccgcaggc
     2221 ggcgtacgcg gccgcgattc gcgaggcgct gcccgatgac ggcatcctgg tcagcgagat
     2281 cacccaggtc ggatactggg ccagctatgg gttcccggtg tatcagccgc ggacgttcat
     2341 caccgcgggc tatcagggga cgctcgggta cgggtttgcc acggcactcg gggcgcaggt
     2401 cgccgccccc ggccggaagg tggtctcgat caacggcgat ggtgggttca tgtacaacgt
     2461 ccaggaactg tcgaccatgg tgcggcaccg gcttccggtg gtggtggtcg tgttcaatga
     2521 caacgcctac ggcaacgtcc gccgcatcca gcgcgagtcc ttcggcgggc gactgatcgc
     2581 gtccgacctg cacaatccgg att
//
LOCUS       DASQGH010000019         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14238, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000019 DASQGH010000000
VERSION     DASQGH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14238"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEW91_00320"
     CDS             complement(<1..706)
                     /locus_tag="VEW91_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90059.1"
                     /translation="MPPILQLRGIDASYGKAQILFGIDLHVLEGEVVAVIGANGSGKS
                     TMLRVITGLLRPTAGTVVFRGEPIDRFPPHTIVARGISMVPQYRRIFGSLTVEENLEI
                     GAYTRHRDRQAFLKFLDEAYRLFPVLAQKRYQLGGVLSGGEQQMLSIARGLMAQPSLL
                     LLDEPSMGLAPKLIVDVFRDIRRIAESGRTVVVVEQNAYAALSIARRGYVLENGRIAL
                     AGAAQDLFHNAYVRKTY"
     gene            complement(724..1719)
                     /locus_tag="VEW91_00325"
     CDS             complement(724..1719)
                     /locus_tag="VEW91_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HYM90060.1"
                     /translation="MTIRTSAVVLPECGRVAVADVDLPTPGEEDLAVRILLSGISAGT
                     ERWVLQGRYGPARFPLIPGYQSVGVVERVGAKVSEFKPGDVVFAGTTRTPEGMHAWWG
                     GHTARAVLPSSRAVRIPPGVSLEEAALSALVAVAHHGVRLLPLRPGAIAAVVGLGMVG
                     QLVAQLLDRAGVVVIGSDPLKARRDRATALGLRQVVDPAAEHLEEAVRALAQDGADVV
                     IECTGLTHLVGSCLRLLRFGGTLSLQGYYPGNIVFPFEPAHRAEATIKCPRGWPRSDH
                     EAALALLAGKGIRVAPLITHRLPAKDAVHAYELLTSHPEDTLGIVLDWDQRSSGP"
     gene            complement(1791..2939)
                     /locus_tag="VEW91_00330"
     CDS             complement(1791..2939)
                     /locus_tag="VEW91_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90061.1"
                     /translation="MKGREWLVSACLVALMFLVATMGGPPPSRGAAPAVTIGVAAPLT
                     GPRALLGRNFRQGVELALDEINEAGGVLGQPLRVIFEDDQGDNPGAAINAVNRLISVH
                     KVPLMLGPHYSVAQMATQKIYCDAKVVSLTGASGVPVTAQGCKYVFRVRASDAIVAPA
                     LARYIKEHLKFTKVAILYVNDDFGKAGAAAAAAALDSVGLKPVAVESHNAGDKDFTAQ
                     LTKIVRAGAESVVTWTHDTDAALVLRQARELNLNLRFAGPTSLSAPSFIALAGEAAEG
                     VFSANDFVPTVPTERVQSFVKRYTRRYKTLPEIWASAYYDATWLAAKAIQSARSTDPD
                     KVRAALLSLRYSGVLADFRFDPNGDGNHQIHIVTVRNGQAEWVTTVKF"
     gene            complement(3028..>3490)
                     /locus_tag="VEW91_00335"
     CDS             complement(3028..>3490)
                     /locus_tag="VEW91_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90062.1"
                     /translation="VVTWTHDIEGALIMRQARELNLNLRFAGTTSVSQPMFIALAGEV
                     AEGVISTAEFVPTVPTERAQAYVKRYTQRWKELPEIWASAYYDSAWLAAKAIQWAEST
                     DPDKVRRALLSHRYSGVLADFRFDPNGDGNHQVHIVQVRNGRAEWVTTVKF"
BASE COUNT          620 a         1176 c         1114 g          580 t
ORIGIN      
        1 gataggtttt gcggacgtag gcgttgtgaa agagatcctg tgcggctccg gccagcgcga
       61 tccgcccatt ctccaacacg tagccgcgcc tcgcgatgct gagcgcggca taggcattct
      121 gctcgaccac gaccacggta cgccccgact ccgcgatccg gcgaatgtcc cggaaaacgt
      181 cgacaatgag cttgggggcc aggcccatcg acggctcgtc gagcaggagc agcgacggct
      241 gggccatcag accgcgggcg atggacagca tctgctgctc ccctcctgaa agaacgcccc
      301 cgagctggta gcgcttctgg gcgagcaccg ggaagagccg gtaggcttcg tccaaaaact
      361 tcaggaaggc ctgacggtcg cgatgccgag tgtaagcccc tatctcgagg ttttcctcca
      421 cggtcaggct tccgaagatg cgccggtact gtggcaccat ggagatcccg cgcgctacga
      481 tggtgtgagg ggggaagcga tctatgggct cgccgcggaa cacgactgtc cctgcggtgg
      541 gacggaggag gccggtgata acccggagca tcgtggactt gcccgatccg ttcgctccga
      601 tgacggccac gacctcgcct tccaggacgt ggagatcgat accgaagaga atctgggcct
      661 tgccgtagga ggcgtcgata ccgcgcagct gcaggatggg cggcatgggg actgtctccc
      721 tcctcaggga ccggaggacc gctgatccca gtccagcacg atccccaggg tatcttcagg
      781 atgcgacgtg agcagctcgt acgcgtgaac ggcatctttg gccggcagcc tgtgcgtgat
      841 cagcggcgcc acgcggatcc ccttcccggc cagcagcgcg agggccgcct cgtggtcgga
      901 cctgggccat cccctcggac acttgatggt cgcctctgcc cggtgggcgg gctcaaaggg
      961 aaagacgata tttcccggat agtatccctg gagggagagt gtcccgccga atcggaggag
     1021 acgcaaacac gaacccacaa ggtgcgtcag gccggtacac tcgattacga cgtccgcgcc
     1081 atcctgcgcc agcgctctga cggcctcctc caggtgctcc gcggcggggt cgaccacctg
     1141 tcggagcccc agggctgtgg cccgatctcg gcgcgctttc aatgggtcag atccgatcac
     1201 cacaacgccg gctcggtcga ggagctgcgc aacgagttgt cccaccatcc ccaacccgac
     1261 aacggccgcg atcgccccgg ggcggagggg gaggagcctg accccgtgat gggcgacggc
     1321 cacgagcgct gacagcgcag cctcctcgag gctcaccccc ggagggatgc gaacggctcg
     1381 agaggagggg agcacagccc tggcggtgtg gcctccccac cacgcatgca ttccctcggg
     1441 ggtgcgggtg gtccccgcga acaccacgtc gccaggcttg aactcggaca ccttcgcgcc
     1501 gacccgttcc accacaccga cgctctggta gcccggaata agagggaagc gggccggacc
     1561 atagcgcccc tgcagcaccc accgctcggt cccggcgctg atcccggaga ggaggatgcg
     1621 aactgccaga tcctcctctc cgggcgtggg aaggtcgacg tcggccacgg ccacgcgccc
     1681 gcactcggga agcaccacgg cgctcgtgcg gatcgtcatg cgccttcctc agatccctgc
     1741 gccgcttcgg cgcatcgtgc ccaccgtacc gcacacgcac gcgcacgcgg tcagaacttc
     1801 accgtggtca cccactcggc ctggccgttg cggaccgtga cgatgtgaat ctgatggttc
     1861 ccatcgccgt tcggatcgaa ccgaaagtcg gccaggaccc ccgagtagcg cagactcagc
     1921 agcgccgcgc ggaccttgtc cgggtcagta cttcgggccg actgaatcgc cttggccgcc
     1981 agccaggtcg catcgtagta ggcggacgcc caaatctcag gcagggtctt gtagcgccgc
     2041 gtataccttt tcacgaacga ctgaacgcgc tcggtcggca cagtggggac gaagtcgttg
     2101 gcgctgaaaa ccccttcagc ggcctccccg gccagggcga tgaagctcgg cgcgctgagc
     2161 gaggtcggcc cggcgaaccg gagattgagg ttgagttcgc gcgcctgccg aaggacgaga
     2221 gctgcgtcgg tatcgtgtgt ccacgtcacc acgctctcgg ctcccgcccg cacgatcttg
     2281 gtgagttgag cagtaaagtc cttatcgccg gcgttgtggc tctccaccgc cactggcttg
     2341 aggcccacgc tgtccagcgc cgcggccgcc gctgcggctc cggccttgcc gaagtcgtcg
     2401 ttcacataga gaatggcgac cttggtgaac ttcaggtgct ccttgatata ccgggcgagc
     2461 gcaggcgcca cgatggcatc cgaggcgcgg acccgaaaga catatttgca gccctgcgcg
     2521 gtcacgggaa ccccactcgc acccgtcagc gagacaacct tcgcgtcaca gtagatcttt
     2581 tgcgtggcca tctgagcgac ggaatagtgc ggcccgagca ttaacggaac cttgtggacg
     2641 gagatcagcc ggttgaccgc attgatggcc gcgcctgggt tatccccttg gtcgtcctcg
     2701 aagatgaccc tgagaggctg gcccaggaca ccccctgcct cattgatctc atcgagcgcg
     2761 agctcaacgc cctggcggaa gttccggccg agcagggcgc gcgggccggt gagcggcgcc
     2821 gccaccccga tggtgaccgc aggagcggcg ccgcgagacg gcggcggacc gcccatcgtg
     2881 gcaacgagga acatcagagc tacgagacat gcgctgacca accactcccg acctttcatc
     2941 cctatccctc ctttggcgag tacccgtccg gatggtccgg caggcactcg ccgaagccca
     3001 tgaccacacg cttccttccg ctcttggtca gaacttcacc gtggtgaccc actcggctcg
     3061 gccgttgcgg acctggacga tgtggacttg atggttcccg tcgccgttgg gatcgaaccg
     3121 aaagtcggcc aaaacgcctg aatagcgatg actcagaagc gcccgacgca ctttgtccgg
     3181 gtccgtgctc tcagcccact ggatcgcctt ggccgccagc cacgccgaat cgtagtacgc
     3241 ggaggcccag atctccggta gctccttcca ccgctgggtg tatcgtttca cgtaggcctg
     3301 ggcgcgctcg gtcggaacag tgggaacgaa ttcggcggtg ctgatgaccc cttcggccac
     3361 ctcacccgcc agcgcgatga acattggctg gctgaccgag gtcgtgcccg cgaaccggag
     3421 gttgagattc agctcgcgcg cctgtcgcat gatcagcgcc ccttcgatgt cgtgcgtcca
     3481 cgtcaccacg
//
LOCUS       DASQGH010000020         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_121, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000020 DASQGH010000000
VERSION     DASQGH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1653
                     /locus_tag="VEW91_00340"
     CDS             277..1653
                     /locus_tag="VEW91_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90063.1"
                     /translation="MAEQEVSRRNFLNQLGVTVGAAALGGAAGPLAAAGPAEAQQPPA
                     GRIPDTPIKVGQMTFLSGPAAVLGEPMLKGTILAADEINAQGGLLGKRKIEIIKADEN
                     AGTDANVKELKRLKLQEKIDLFTGITSSGNTPALGPVAEELKALTIFVDGCTDFLFDK
                     AVPNPKYVFRITNIQSADGGTCALAVVATWPFVRKVAHIHPDYSYGRNAFDHIRIVMD
                     KMLPGVKIVMEGWPKLGTTDFAAHITKAIAANPDILVSSVWGGDYVAFYKQAIGYGLF
                     NRMKYATTLALGVAPHAIGKDHPVDVIGGVHANYYFTYPGGGKWPPNTAFVKSYYARW
                     KEYPNFQAEGAYVAMQMYKMAIERANKLMGGWPDDEAIIGQLEGLGYAGPAGYVYFRP
                     DNHQGYKDAMTGFTKNYDSYPFQVLDPNRVITIPIRNITAPPGWPQGEPTRTYDWINK
                     TWPKITGA"
     gene            1745..>2752
                     /locus_tag="VEW91_00345"
     CDS             1745..>2752
                     /locus_tag="VEW91_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90064.1"
                     /translation="MARTRAVAAAGVLALVAAAARVEAGHESPFYPSYYPQEIRLDAV
                     APAAARRLLANASIHAYVGGDPFAGRRAPAGVALVESLGSYVVVTPNPASRRLATPAA
                     RGEAAADVIRRLDRQHGAFVFSPYPVTPYHWDYLEHADLAAAALHGLPAAAQGGRPPN
                     RSTIVVATGEAAGLIGAEASHAPQGSPDWDVRVETIDADELLAPSAIRFAGWVGPPWL
                     KAGWFDAYLLMGGSVADHERRAEVDHLYHRLVTGGYRGVEDRVNLERTLVSRLRQGCE
                     RVVVGYTLRREYYNTDYSNGIENVGFDSHSGFNSAVFIRTAKLKDFPWNGWLRLGVAG
                     TP"
BASE COUNT          483 a          868 c          991 g          410 t
ORIGIN      
        1 atgggctggg tcgtgagtgt ccagggagag cgatcgcggc cggcgcgcgg gccgagcggc
       61 ggggcgtcgg agggcgtgcc ccccctgccg gtacgcggtg ggtagaggtg ggggcgcggc
      121 gggaacgaat atccatccag gacaacgcag cgggaatgtg tggtcgaaag gagggatgag
      181 ctcaacgcgc aggctcagcg tgaggatcct tccgagatgc tcgatcgctg gcgcgaatgg
      241 acgagcagcc gatgtgaggg agagggggat gacaccatgg cagaacagga agtgagtcgc
      301 cgcaattttc tcaaccaact cggcgtgaca gtgggggcgg cggccctggg tggcgcggcc
      361 ggaccgttgg ccgcggccgg accggcggag gcccagcagc cgccggccgg gcggatcccc
      421 gacacgccca tcaaggtcgg gcagatgacg ttcctgtccg ggccggccgc ggtcctcggc
      481 gagcccatgc tcaaggggac gatcctggcc gccgacgaga tcaacgccca agggggattg
      541 ctggggaagc ggaagatcga gatcatcaag gccgacgaga acgcggggac cgacgccaac
      601 gtcaaggagc tcaagcgcct gaagctccag gagaagatcg acctgttcac gggaatcacg
      661 tccagcggca acacgcccgc gctcggcccg gtggccgagg agctcaaggc cctcacgatc
      721 ttcgtggatg ggtgcaccga cttcttgttc gataaggccg tgccgaaccc caaatacgtc
      781 ttccggatca cgaacatcca gtcggcggat ggggggacgt gcgcgctcgc ggtggtggcg
      841 acctggccgt ttgtgcggaa ggtggcacac atccaccccg actactcgta cgggagaaac
      901 gccttcgatc acatccgaat tgtgatggac aagatgctgc cgggggtgaa gatcgtgatg
      961 gaggggtggc cgaagctcgg caccaccgac ttcgccgccc acatcaccaa agcgatcgcg
     1021 gcgaacccgg atatcctcgt ttcgtcggtg tggggcgggg actacgtcgc cttctacaag
     1081 caggccatcg gctacggcct gttcaacagg atgaagtacg cgaccaccct ggcgctcggc
     1141 gtggcgccgc acgcgatcgg gaaggaccac cccgtggacg tcatcggcgg ggtccacgcc
     1201 aactactact tcacctaccc gggcgggggc aagtggcccc ccaacaccgc gttcgtcaag
     1261 agctactacg cccggtggaa ggagtatccc aacttccagg ccgagggcgc ctacgtcgcg
     1321 atgcagatgt acaagatggc gatagagcgg gccaacaagc tcatgggggg ctggcccgac
     1381 gacgaggcga tcatcggcca gctggagggg ctcgggtatg ccggccctgc ggggtacgtg
     1441 tacttccgcc cggacaacca ccagggatac aaggacgcga tgacgggctt tacgaagaac
     1501 tatgacagct acccgttcca ggtcctcgac cccaaccggg tcatcacgat cccgatccgg
     1561 aacatcaccg ccccgcccgg gtggccgcag ggcgagccaa cccggacgta tgactggatc
     1621 aacaagacct ggccgaagat tacaggagcc tgagtctgtg gtggggcggg gcccgcgggc
     1681 aacccgcggc cctgccccgg gctgaccttc cggcagcctc gggggcggtg ggagatggga
     1741 tgacatggcc cgtaccaggg cggttgcggc cgctggagtc ctcgcgctcg tcgcggccgc
     1801 agcgcgtgtc gaggccggcc acgagtcccc gttctacccg tcgtattacc cgcaggagat
     1861 tcgcctggat gcggtggccc cggcggccgc caggcgcctg ctggcgaacg cgtcgatcca
     1921 tgcctacgtg ggcggcgacc cgtttgccgg ccgccgcgcg cccgccggcg tcgccctggt
     1981 ggagtccctg ggctcgtatg tcgtcgtaac cccgaacccc gcatcgagac ggctggccac
     2041 cccggcggcc cggggtgagg cggcggcgga tgtgatccgc aggctcgacc ggcagcacgg
     2101 cgcatttgtc ttctctccct atccggtgac gccgtaccac tgggattacc tcgagcacgc
     2161 cgacctcgcc gcggccgcgc tccacggtct ccccgcggcg gcccagggag gtaggccgcc
     2221 gaaccggagc acgatcgtgg ttgcgacggg ggaagcggca ggcctcatcg gcgccgaggc
     2281 gtcccatgca ccccaagggt cgccggactg ggatgtccgg gtggagacca ttgatgccga
     2341 cgagctgctc gcgcccagcg cgatccgatt tgcggggtgg gtgggcccgc cgtggctcaa
     2401 ggcgggatgg ttcgatgcct accttttgat gggcggaagc gtcgccgatc acgagcggcg
     2461 ggcggaggtc gatcacctgt atcaccgctt ggtgacgggt gggtatcggg gcgtggaaga
     2521 ccgggtgaac ctcgagcgga cgctagtctc gcggctccgg cagggatgcg agcgtgtggt
     2581 cgtggggtac acgctgcggc gggagtacta caacaccgac tattccaacg gcattgagaa
     2641 cgtgggattc gactcgcaca gcggattcaa ctccgcggtg ttcatccgga ccgccaagct
     2701 gaaggacttc ccgtggaacg gctggctgcg ccttggggtg gcggggacgc cc
//
LOCUS       DASQGH010000021         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53875, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000021 DASQGH010000000
VERSION     DASQGH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53875"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VEW91_00350"
     CDS             <1..503
                     /locus_tag="VEW91_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HYM90065.1"
                     /translation="KGLDPADVLRHKEQTGTVSGYRRGDAVTNEEVLELPCEILVPSA
                     LEGQITGKNAPRVKARIIVEGANGPTTPEADRILRDQKVLVVPDILANAGGVTVSYFE
                     WVQDLQSFFWTEEEINERLERIMVRSFREVLAVSQERKVDLRTAALVRGVGRVAEALM
                     TRGIYP"
     gene            523..1452
                     /locus_tag="VEW91_00355"
     CDS             523..1452
                     /locus_tag="VEW91_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HYM90066.1"
                     /translation="MLRPILLALSDKRGLARLALRHPVLRRAALRFVAGERLDEAVSA
                     IRGLNAAGLAATLDHLGEHTTTRSGAEASAGAYLAILDELHRTGVDSNLSLKLTQLGL
                     DLDETLCAAHLGRILERAGDTFVRIDMESSQYIEPTLRLFERLWGAGRRNVGVVIQSY
                     LHRSPSDVERLIALGARVRLVKGAYAEPPSVAFPLKRDVAAAFARLTEQLLRRGVYPA
                     IATHDERLIAAASQVATAHGIARDRFEFQMLYGIRRDLQVRLHREGYRVRVYVPFGGE
                     WYPYFMRRLAERPANVMFVIGSLVRERGGRGQG"
     gene            1611..2801
                     /locus_tag="VEW91_00360"
     CDS             1611..2801
                     /locus_tag="VEW91_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYM90067.1"
                     /translation="MVALLSGLAVLGLVLMIPGTGGVHPVSAAAMKTVKIGVDLPMSG
                     GEAPNGVPTNKGVILAIDEANKAGGPYNFEEVLKDDAVNGVHDPAQGAKNMQELIADA
                     AVVGIVGNFNSNVGKASIPITNDADLVQISPSQTNPTLTKGPDAAALRKKANNYFRVC
                     PTDDLQGPVAADYAYKELKARKAAIIDDQETYGKGIADEFEKEFKKLGGTVVSHDGIP
                     KGTQDFHAILTSIRAKNPDILFFGGVTTTGGGLIRKQMPDVGMKTTYEGGDGIVEDEF
                     LKVAGDAANGSYGTVASVNAEKIAAAQGFITSYKASFKDDPGAYSANAYVAARIIIDA
                     VKAVGPDRAKVRAWVANLRNYKSIIGTFSFDKNGDTTNKIISVYQVRNGKWDWITQVS
                     FTGG"
     gene            2863..>2994
                     /locus_tag="VEW91_00365"
     CDS             2863..>2994
                     /locus_tag="VEW91_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90068.1"
                     /translation="MHIDWALLISQLINGLSLGAIYAVIALGYTMVYGVLEFINFAHS
                     "
BASE COUNT          552 a          934 c         1056 g          452 t
ORIGIN      
        1 agaagggcct cgatcccgca gatgtgctcc gccacaagga gcagaccggg acggtgtcgg
       61 ggtaccgcag gggcgacgcc gtgacgaacg aggaagtcct ggagctcccg tgcgagatcc
      121 tggtgccgag cgcgctcgag gggcagatca ccggaaagaa cgctccccgg gtcaaggccc
      181 ggatcatcgt ggagggggcc aacgggccga cgacccccga ggccgaccgg atcctccggg
      241 atcagaaggt gctggtggtc ccggacattc tcgccaacgc cggcggggtg accgtgtcct
      301 acttcgagtg ggtgcaggac ctgcagtcgt tcttctggac cgaggaagag atcaacgagc
      361 ggctcgagcg gatcatggtc cggagcttcc gcgaggtgct cgcggtctcc caggagcgca
      421 aagtggacct gcggacggcg gcgcttgtcc gcggcgtcgg ccgggtcgcc gaggcgttga
      481 tgacccgcgg catctacccg tagcgctcat cggccgggcc cgatgctgcg gccgatcctg
      541 ctcgcgctct ccgataagcg ggggctcgcg cggttggccc tccgccaccc cgtcctccgg
      601 cgggcggcgc tgcgcttcgt cgccggggag cggctggatg aggcggtgtc cgcgatccgc
      661 gggctgaacg cggccgggct tgcggcgacg ctcgaccacc tcggggagca caccacgacc
      721 cggagcgggg cggaggcgag tgccggggcc tacctcgcca tcctggatga actgcaccgc
      781 accggcgtcg acagcaacct ctccctgaag ctgacccagc tcgggctgga tctcgacgaa
      841 acgctgtgcg cggcgcacct cggccggatt ctggagcggg cgggggacac cttcgttcgc
      901 atcgacatgg agagttcgca gtacatcgag ccgaccctca ggctcttcga gcgactgtgg
      961 ggggcgggac gccggaacgt cggggtcgtg atccaatcgt acctccaccg cagcccatcc
     1021 gacgtggagc ggctgatcgc cctgggagcg cgggtgcgcc tggtgaaggg ggcatacgcg
     1081 gagccgccct cggtggcgtt ccccctgaaa cgcgacgtcg ccgccgcctt tgcccggctg
     1141 accgagcaac tcctccgccg gggcgtctac ccggcgatcg ccacgcacga cgagcggctc
     1201 atcgccgcgg ccagccaggt cgccacggcc catgggatcg cccgcgaccg gtttgagttc
     1261 cagatgctct acggcatccg gcgggacctc caggtgaggc tccaccggga ggggtaccgg
     1321 gtcagggtgt atgtcccgtt tggtggggag tggtacccgt acttcatgcg ccggcttgcg
     1381 gaacgcccag ccaacgtcat gttcgtgatc gggagcctgg tgcgggagcg gggtgggcgc
     1441 ggccaggggt aagggggtgg gggtgaaggg aaccattcct ccctccaaga acgtacgagg
     1501 cgaggggaag cggagaaggc ggccggggcc cgtggaccgg cccgtccagg caccgcggct
     1561 ctgctgtacc agtggtgcgt tgcagaaaag ggggggagaa catgcgaaga atggtggcac
     1621 tgctgagtgg cctcgccgtt ctcgggcttg tcctgatgat ccccgggacc ggaggggtgc
     1681 atccggtcag cgcggcggcg atgaagacgg tcaagattgg cgtcgacctc ccgatgtcgg
     1741 gaggggaagc gcccaatggc gtgccgacga acaagggcgt tatcctggcg atcgacgagg
     1801 ccaacaaagc cgggggtccc tataatttcg aggaagtcct caaggatgac gccgtcaacg
     1861 gcgtgcacga cccggcccag ggcgccaaga acatgcagga gttgatcgcc gatgccgcgg
     1921 tcgtcggcat agtcggcaac ttcaacagca acgtggggaa ggcgtccatc ccgataacga
     1981 acgacgccga cctcgtccag atcagcccgt cccagacgaa ccccacgctc accaagggac
     2041 cggatgcggc tgcgctccgg aagaaggcaa acaattactt ccgggtctgc cccaccgatg
     2101 acctccaggg cccggtcgcg gccgactacg cgtacaagga actgaaggcg cggaaggccg
     2161 cgatcatcga cgaccaggag acgtatggca agggaatcgc cgacgagttc gaaaaggagt
     2221 tcaagaagct cggcgggacc gtcgtgagcc atgatggcat ccccaagggc actcaggact
     2281 tccacgcgat cctgacgtcg atcagggcca agaaccccga tatcctgttc ttcggcggcg
     2341 tgaccacgac cggcgggggt ctgatccgca agcagatgcc ggacgtcggg atgaagacca
     2401 cctatgaggg tggcgacggt attgtcgagg atgagttcct gaaggtggcc ggagatgcgg
     2461 cgaacgggtc ctacggcacc gtggcgtcgg tgaatgccga gaagatcgcg gcggcccagg
     2521 ggttcatcac atcgtacaag gcctcattca aggacgaccc aggcgcgtac agcgcgaacg
     2581 cgtatgtcgc ggcgaggatc atcatcgacg cggtgaaggc cgtgggtccc gaccgggcga
     2641 aggtccgggc atgggtcgcg aatctaagga actacaagag catcatcggg accttctcgt
     2701 tcgacaagaa cggggacacg acgaacaaga tcatcagcgt ttaccaggtc aggaacggca
     2761 agtgggactg gatcacgcag gtgagcttca ccggaggata agcacccgca atctctcctg
     2821 aggcgcgggg ggcggctatg gccgccccgc gccccgcatg cgatgcatat cgactgggcc
     2881 ctcttaatct cgcagctcat caacgggctc tccctggggg cgatttacgc ggtcatcgcc
     2941 ctcgggtaca ccatggtcta cggggtgctc gagttcatca acttcgccca ctcc
//
LOCUS       DASQGH010000022         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34043, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000022 DASQGH010000000
VERSION     DASQGH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34043"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VEW91_00370"
     CDS             complement(<1..409)
                     /locus_tag="VEW91_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HYM90069.1"
                     /translation="MPGPEGIHTQVMAAGRRFVGDLAGGRAAHGDRYLLRVVQPALLG
                     LMDGSISTLAPLFASAFATRNPHTAFLVGGAAALGAGISMGFAEALSDTGKLTDRGHP
                     VIRGTITGVATLIGGLLHTFPFLIPHLGLALAVA"
     gene            complement(409..879)
                     /locus_tag="VEW91_00375"
     CDS             complement(409..879)
                     /locus_tag="VEW91_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYM90070.1"
                     /translation="MSDHGPADGPARPWADLRRRLGAQGCRLTPQRMAIYEAVVGRTS
                     HPSVAAVHEEVSRRFPGLSPATVYSTLELFARLGLVQEISGPVRRYDGRAGVHINLVC
                     HRCGQVLDVADSGLKALQRRAAARARFQVSAVRFELHGLCPRCRRRPGRRNGER"
     gene            complement(908..2074)
                     /locus_tag="VEW91_00380"
     CDS             complement(908..2074)
                     /locus_tag="VEW91_00380"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HYM90071.1"
                     /translation="MRRLTILGSTGSIGRTALDVARRLVGEITVVGLSACTNVALLAR
                     QVRDVGPEAVAVGSAEGARALRDAVPGWRGDVLIGPEGLAALAGGTPADLVLVGVVGV
                     AGLEPTLAALSAGKDIALATKEVLVAGGALVTSAATQRGRRILPIDSEHSAIFQCLAG
                     QDAGAVARLWLTASGGPFLHLDREAMEAVTPADALRHPTWQMGPKVTVDSATLMNKGL
                     EVIEAHWFFGVPASKIEVVIHPQSLVHSLVEFVDGSLLAQLGAPDMHLPVQYALTYPR
                     RLPATGTRLDLRTLGTLGFEPPDPVRFPCLGLAREALEMGGTAPAALNAANEVSVRQF
                     LEGRIRFPEIARNVRRVLDRHTPAPATSVVAVLAADREARAQLTQRSGSAKFIN"
     gene            complement(2100..2594)
                     /locus_tag="VEW91_00385"
     CDS             complement(2100..2594)
                     /locus_tag="VEW91_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYM90072.1"
                     /translation="MRAKGTRPAQFPRPSASEDLAPMKVFTTTLTVPTRERTEICNLT
                     DQLAALPALQQISHGVVLLHSLHTTTGLCLNEFQEALLHDIKELLRRVIPDEDVYRHN
                     DPAFSDDTRGNAASHLSGILLGQTLQIPIEHGRLKLGTWQSVLFCEFDGPQTRQVYVQ
                     VMGV"
     gene            2774..>3367
                     /locus_tag="VEW91_00390"
     CDS             2774..>3367
                     /locus_tag="VEW91_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYM90073.1"
                     /translation="MRLVTFSARGQTRMGAEAGEWIIDLNRGARAAATAARVRALLPP
                     SMEEFLAGGEEAMATARDVISAIRGRLGAPREVTRLQREGVVWAPGEIRRLPPVPAPP
                     KILCVGRNYAEHAREGGSEPPELPIFFGRFAHSLLGPGEPFVLPGVSPQVDFEGELVA
                     VIGTGGREIPEARALESVAGYTVFNDISVRDFQRRTSQ"
BASE COUNT          517 a         1207 c         1159 g          484 t
ORIGIN      
        1 acgcgaccgc aagcgcaagc ccgaggtgcg ggatgaggaa cgggaacgtg tgcagcagac
       61 cgccgatgag cgtcgccacc cccgtgatcg tcccccggat caccggatgt ccccggtcgg
      121 tgagcttccc ggtgtccgag agcgcctcgg caaaccccat gctgatgccg gcaccgagcg
      181 cagccgctcc gccgaccagg aaggccgtgt gcgggttgcg cgtcgcgaac gcgctcgcaa
      241 acaggggtgc gagggtggag atcgacccgt ccatcagtcc caacagcgcc ggctgcacga
      301 ccctgagcag atagcggtcc ccatgggccg cgcggccgcc cgcgaggtcc ccgacaaacc
      361 ggcggccggc tgccatcacc tgggtgtgga tcccctcagg acccggcatc agcgctcccc
      421 gtttcgacgt cctggccgac ggcggcagcg ggggcagagc ccgtgcagct caaaccgcac
      481 cgccgacacc tggaacctcg cgcgcgcggc ggcccgacgc tgcagggcct tgagcccgga
      541 atccgccaca tcgagcacct ggccgcaccg gtggcacacc aggttgatgt gcacccccgc
      601 ccggccatcg tagcggcgca ccggcccgct gatctcctgg accagcccca gccgggcgaa
      661 cagctccagg gtcgagtaga cggtcgcggg gctcagcccg ggaaaccgtc ggctgacctc
      721 ctcgtgcacc gccgccacgc tgggatggct cgttcgcccc accaccgcct cgtagatcgc
      781 cattcgctgc ggggtcaggc ggcacccctg cgcgcccagg cgtcggcgaa ggtcggccca
      841 cggccgcgca ggtccgtccg cgggcccgtg gtcagacatt tgaactagat ccttattaat
      901 agccatttca attaataaat ttagcggatc ccgaccgctg tgtcaactgc gcccgggcct
      961 cgcggtcggc ggccaagacg gccaccaccg acgtggccgg ggccggcgtg tggcggtcca
     1021 ggacccggcg gacgttccgg gcgatctcgg ggaaccggat ccgtccctcg aggaactgcc
     1081 tgaccgagac ctcgttcgcc gcgttgaggg cggccggcgc ggtcccgccc atctcgaggg
     1141 cctcccgcgc taagcccagg cagggaaacc ggaccggatc cggcggctcg aagccgaggg
     1201 tgccgagggt gcgcagatcc aaccgggtcc cggttgccgg gagccgccgc ggatacgtca
     1261 gcgcgtactg gacgggcagg tgcatgtcgg gagcgccgag ttgcgcgagc agcgatccgt
     1321 cgacgaactc cacgagcgag tggaccaggc tctgcggatg gatcacgacc tcgatcttgc
     1381 tggcgggaac gccgaagaac cagtgggctt cgatcacctc cagccccttg ttcatcagag
     1441 tcgccgagtc gaccgtcacc ttcggcccca tctgccacgt gggatgccgc aacgcgtcgg
     1501 ccggggtcac ggcctccatc gcctccctgt cgagatgcag gaaggggccg ccggacgccg
     1561 tgagccacag ccgtgcgacc gccccggcat cctggccggc gaggcactgg aagatcgccg
     1621 agtgctcgct gtcgatcggg aggatccgcc ggccccgctg ggttgcggcg ctggtcacga
     1681 gggctccccc ggccaccagc acttccttgg tcgccagcgc gatgtccttc cccgcggaca
     1741 gcgcggcgag cgtcggctcg agaccggcca ccccgaccac cccgaccaag acaaggtccg
     1801 cgggggtccc cccggccagg gcagccaatc cctctgggcc gatcagcacg tccccccgcc
     1861 acccggggac cgcgtcccgg agtgcccgcg cgccctcagc cgacccgacc gcgacggctt
     1921 cggggcccac gtcccgcacc tgtcgggcga gcagcgccac attggtgcag gccgagaggc
     1981 ccacgaccgt gatctctccg acgaggcggc gggcgacgtc cagggccgtc cggccgatcg
     2041 accccgtcga tccgaggatg gtcaggcggc gcatcgatgg gcgatgcgtc gggacggcac
     2101 tagaccccca tcacctgcac gtagacctgc cgcgtctgcg ggccgtcgaa ctcgcagaaa
     2161 agaacgcttt gccacgtgcc cagcttgagc cggccatgct cgatcgggat ctggagcgtc
     2221 tgtccaagga ggatgccgct gagatgactc gccgcgttcc cgcgggtatc gtccgagaac
     2281 gccggatcgt tgtgccggta cacgtcttcg tccgggatga cgcgccggag cagctccttg
     2341 atgtcgtgca acagcgcttc ttgaaattca ttgaggcata agccggtcgt ggtgtggagg
     2401 ctgtgcaaca ggacgacccc atgcgagatc tgttgcaggg ccgggagcgc ggccagctgg
     2461 tcggtgagat tgcagatctc cgtccgctcc ctggttggca ccgtcagggt cgtggtgaac
     2521 accttcatcg gagccaggtc ctcgcttgcg gacggtcgtg ggaactgcgc gggtcgggtg
     2581 cctttcgctc tcatgccgcg ttcctccatc tcagcgccga tgccctccct ttccaggacc
     2641 cccaccaggg ccgcaccgta ccattatgca cgatctcacg cggtttgtcc tggcagggtc
     2701 gccctccccc ggaccggagc gcgagagtgg ggcggccttt gaagaccgcc aataggtccc
     2761 ccggaggtga ccgatgcgcc tggtaacgtt ttcggctaga ggacagacgc ggatgggggc
     2821 cgaggcggga gagtggatca tcgacctcaa ccgcggggca cgcgccgccg ccacggcggc
     2881 gcgtgtgcgc gcgctgctcc cgccgtcgat ggaggagttc ctggccggcg gggaggaggc
     2941 catggcgacc gcccgggacg tgatcagcgc gatccggggg cgcctcggag cgccgcgcga
     3001 ggtgacgcgg ctgcagcgcg agggcgtcgt gtgggcgccc ggcgagatcc gacgcctgcc
     3061 gccggttccc gcgccaccga agatcctctg cgtcggccgg aactacgcgg agcacgcgcg
     3121 ggaaggcgga tccgagccgc ccgagctgcc gatcttcttc ggccggttcg cccacagcct
     3181 gctcggcccg ggggagccgt tcgtcctgcc cggcgtgagc ccgcaggtcg acttcgaggg
     3241 cgagctcgtc gccgtcatcg ggacgggcgg gcgcgagatc cccgaagccc gcgcgctcga
     3301 atctgtcgcc ggatacacgg tcttcaacga catctcggtc cgggacttcc agcggcgcac
     3361 ctcgcag
//
LOCUS       DASQGH010000023         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31247, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000023 DASQGH010000000
VERSION     DASQGH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31247"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VEW91_00395"
     CDS             <1..1196
                     /locus_tag="VEW91_00395"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90074.1"
                     /translation="VETPSEADEALALADAAPDPVVSAFTPSDAQVLNLLARAPVREA
                     EAILTRSFAAFQRRPQIAPIRARLEALPPDPLTTRPCGDRVTTRGRFDHLLRRLRRER
                     RRRPDTTRQAAGSPAAAPAAQVADVGRALLEMPCTGCPVVQQCLATLKDLRAVERRRY
                     ALAAELSDLEDHEIRVFRRRAAILRDLGYLDSGGRLTPQGRIAARIRHPRMLILAEAL
                     RSRILPADPVALAAVSGALGTERVGPPTRRGDVHHRGRDLRHPGARTLPDLAIGATGA
                     ALDQASWHEARKALLALRSIVAGLNKRRAAAGLPQDPVEREFDPVGGGPAPTFRRAAV
                     VGAWASGRPWEEVVTRHRIPEGDLQRLVWQAAEILAQLEESPHGPLAAAARVARGAML
                     RTPVL"
     gene            1345..2544
                     /locus_tag="VEW91_00400"
     CDS             1345..2544
                     /locus_tag="VEW91_00400"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYM90075.1"
                     /translation="MTTAAGPLAGLTVLDLSTIVSGGTTTAMLADLGARVIKIEHPAG
                     GDPLRSWGPFISGMSVWWKVVSRNKKSITLNLSRPRGQQILTDLLHHADALVENFRPG
                     TLERWGLAPAALLERNPRLVILRISGFGQTGPYRHRPGFGTIAEAMSGLVALSGYPDA
                     PPLLPPLPMADEIAGLTGALAVVAAIHHRARSGRGQVIDVSLYEPLFRLLIPYVTQYT
                     ALGILAQRTGNSFSDAAPRNLYRAADGTWIALSATSQRVFERLAAAIGRPDLAQDPRF
                     RDNTARVGHREELDAIIAAWMAARRQDEILAQLEESGAVAGPVYDIPRILADPQYAAR
                     EDVIAVRDPDLGEIKMVGVVPKFSETPGAVTHPGPRLGEHNHEVYGGWLGLDDAALAR
                     LAEDGVV"
     gene            2563..3405
                     /locus_tag="VEW91_00405"
     CDS             2563..3405
                     /locus_tag="VEW91_00405"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HYM90076.1"
                     /translation="MRTLDATAITQAIRDLCITVNHRMGPDMLAALERARAMEESELG
                     RSVLDRLLENARVAREHWYPICQDTGTAVIFADLGQDLHVTNGDLERAINEGVRQGYR
                     DGYLRASIVRGPLDRVNTQDNTPAIVYYRIVPGDRLELALLAKGAGCDNMSRVAMLTP
                     SDGYAGVTEFVVRCVSEAGPNPSPPLIVGVGMGGTFERAAVLSKRALLRPVGEPNPDP
                     PLADLEADLLRRINDLGIGPAGFGGRVTALAVHVEAHSTHIASLPVAVNLDCHSHRAG
                     RVIL"
     gene            3405..>3611
                     /locus_tag="VEW91_00410"
     CDS             3405..>3611
                     /locus_tag="VEW91_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HYM90077.1"
                     /translation="MGRPIPLRVPFGPEAADRLRAGDEVLLSGVVFTARDAAHERLSH
                     LLEAGKPLPIDLRGQVIYYCGPTPA"
BASE COUNT          499 a         1366 c         1254 g          492 t
ORIGIN      
        1 ccgtcgagac gccctcggag gccgacgaag ccctcgcgct cgccgacgcc gccccggatc
       61 ccgtggtctc cgcgttcacg ccgtcggacg cccaggtgct gaacctgctc gcgcgggccc
      121 cggtccggga ggccgaggcc atcttgacga gatcgtttgc ggcgttccaa cggcgtcccc
      181 aaatcgcccc gatccgcgcg cgcctcgaag cgctcccacc cgatccgttg acaacccgcc
      241 cgtgcgggga ccgcgtgacc acccggggcc ggttcgacca cctgcttcgg cgcctcaggc
      301 gggagcggcg caggcggccc gatactacgc gacaggccgc gggcagcccg gccgccgcgc
      361 cggcggccca ggtcgccgac gtcgggcgcg cgctgctgga gatgccctgc accggctgcc
      421 cggtggtcca gcaatgcctc gccacgctca aggacctgcg ggcggtcgag cgcaggcggt
      481 acgcgctcgc cgccgagttg tccgacctgg aggaccacga gatccgggtg ttccggaggc
      541 gcgccgccat cctgcgcgac ctcggctacc ttgattccgg ggggcggctg accccgcagg
      601 ggcggatcgc cgcgcgcatc cgtcacccgc ggatgctgat cctggcggaa gcgctccggt
      661 cccgcattct cccggccgac cccgtggcgc tcgcggcggt gagcggcgcg ctcggcaccg
      721 agcgggtcgg tccgcccacg cgccggggcg acgtgcacca ccggggccgc gatctccggc
      781 atccgggtgc tcggacgctg ccggacctgg cgatcggcgc gaccggcgcg gcgcttgacc
      841 aggcgtcctg gcacgaggcc cggaaggcgc tcctcgccct ccgcagcatc gtcgccgggc
      901 tcaacaaacg ccgggccgcc gccgggttgc cccaggatcc ggtcgagcgc gagttcgatc
      961 cggtgggagg tggtccggcc ccgacgttcc gacgcgcagc ggtggtcggc gcctgggctt
     1021 ccggccgccc gtgggaggag gtcgtcaccc ggcaccgcat ccccgaaggc gatctccagc
     1081 ggctcgtctg gcaggccgcc gagatcctcg cgcaactcga agagtctccg cacgggccgc
     1141 ttgcagcagc cgcgcgcgtt gcccgcgggg cgatgctgcg gaccccggtc ctgtgaaaca
     1201 tcgcacgccc ggacggaggc gggcacacct cgacccaccg ctcgggcgcg tgcggcaggg
     1261 acacccccgc acgcacggga atcacttacg cgcatccggc ggatcgacgc gcgttcgcgt
     1321 gtagtgcccg gccgccatgc accgatgacc accgccgccg gtccactcgc tggcctgacc
     1381 gtcctcgacc tctccacgat cgtctcgggg ggcaccacca ccgccatgct cgccgatctc
     1441 ggggcccggg tcatcaagat cgagcatccg gcgggaggtg accccctgcg gtcgtggggc
     1501 ccgttcatct cggggatgtc ggtctggtgg aaggtcgtct cgaggaacaa aaagtcgatc
     1561 accctgaacc tcagccggcc gcgcgggcag cagatcctga ccgacctgct ccaccacgcg
     1621 gacgccctcg tcgagaactt ccgccccggc accctggagc gctgggggct cgccccggcg
     1681 gcgctcctcg agcggaaccc gcggctggtg atcctccgga tctcggggtt cgggcagacc
     1741 ggcccgtacc ggcaccgccc ggggttcggc acgatcgcgg aggcgatgag cggcctcgtg
     1801 gccctcagcg ggtaccccga cgctccgccc ctgctgccgc cgctgccgat ggccgacgag
     1861 atagcgggac tgaccggcgc gctcgccgtg gtggcggcga tccaccaccg cgcgcgctca
     1921 ggccgcgggc aggtgatcga cgtgagcctc tacgagccgc tcttccggct gctcatcccg
     1981 tatgtgacgc agtatacggc gctcgggatc ctggcccaac gcacgggcaa ctccttctcg
     2041 gacgccgcgc cccgcaacct ctaccgggcc gcggacggga cctggatcgc gttgtcggca
     2101 accagccagc gggtcttcga gcggctggcg gcggcgatcg gccggccgga cctggcccag
     2161 gatcccaggt tccgggacaa cacggccagg gtcgggcatc gcgaggaact cgacgccatc
     2221 atcgccgcgt ggatggccgc gcgccgacag gacgagattc tggcgcagct cgaggagagc
     2281 ggcgccgtgg ccggtcccgt gtacgacatc ccgcggatcc tcgcggatcc ccagtacgcc
     2341 gcacgcgaag atgtgatcgc ggtccgggac ccggacctgg gggagatcaa gatggtgggg
     2401 gtcgtcccga agttctcgga gacgcccggt gcggtcacgc accccgggcc gcggctcggc
     2461 gagcacaatc acgaggtcta tgggggctgg ctcggccttg acgatgccgc gctcgcgcgt
     2521 ctcgccgagg acggcgtggt ctagggaggc cgcgacccac ccatgcgaac gctcgacgcc
     2581 accgccatca cccaggcgat ccgagacctc tgcatcacgg tcaaccaccg gatggggccg
     2641 gacatgctgg ccgccctcga gcgcgctcgg gcgatggagg aatccgagct cggccggtcg
     2701 gtcctcgacc ggctcctgga aaacgcccgg gtggcccggg agcactggta cccgatctgc
     2761 caggacaccg gcacggcggt gatcttcgcc gacctcggcc aggacctcca cgtgaccaac
     2821 ggggacctcg agcgcgcgat caacgagggg gtgcggcagg gataccggga cggatacctg
     2881 cgcgcatcga tcgtccgcgg accgctcgac cgggtcaaca cccaggacaa caccccggca
     2941 atcgtctatt accggatcgt gcccggagac cggctggaac tcgcgctgct cgccaagggc
     3001 gccggctgcg acaacatgag ccgggtggcg atgctcaccc cctccgacgg ctacgcgggc
     3061 gtcacggagt tcgtcgtccg ctgcgtttcc gaggcgggcc ccaacccgag cccaccgctg
     3121 atcgtcgggg tggggatggg cgggacgttc gagcgggccg ctgttctgag caaacgcgcg
     3181 ctccttcggc cggtcggcga gcccaacccc gatccccccc tcgccgacct cgaggccgac
     3241 ctcctccgcc ggatcaacga cctcggcatc ggcccggcgg ggttcggggg ccgtgtgacc
     3301 gcgctggcgg tccacgtcga ggcgcactcc acgcacatcg cgagcctccc cgtcgctgtg
     3361 aatctggact gccattccca ccgcgccggc cgggtgatcc tgtgatgggc aggccaattc
     3421 cccttcgggt cccgtttggc cctgaggcgg cggatcggct ccgtgccggg gacgaggtcc
     3481 tgctttccgg ggtggtcttc acggcgcgcg acgcagccca cgaacgtctc tctcacctcc
     3541 tcgaggccgg caagcccctg cccatcgacc tccggggcca ggtcatctac tactgcggcc
     3601 cgacccccgc c
//
LOCUS       DASQGH010000024         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36901, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000024 DASQGH010000000
VERSION     DASQGH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36901"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2148)
                     /locus_tag="VEW91_00415"
     CDS             complement(<1..>2148)
                     /locus_tag="VEW91_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYM90078.1"
                     /translation="QPFVLKFDVSNIPVAQVVVRGAGLDARQLYDLAYNTIEPQLERL
                     PGVSTAFVNGGLVRQLSVSVDPHKLAAAGLALQDVQNAIVRYNQILPSGSLRNNRIDY
                     QLNVPSLLQSVPAIQDVVVATRGGVPVHIRDVARVSDAAADQTQIVNVDGKPGVVMFV
                     ARQPDANTVQTVNAIRAALPSLTGLPPGVTLSVGFDQSRYIRAAIKTLGNEAILGAVL
                     TFLVVLVFLRSLWSLFIVGLGIPLSVSSALLLLYFTGQTLNVFTLGGLTLAMGRLVDD
                     AIVVRENITRHLATPGRSVLEAVLTATQEVGLPVLASTATTIAVFFPVVFLSGIAQRL
                     FVPMALTIVFALAASYVVSMTIDPVLSIKLLRAGTANTEGSGVVARFNRWSEGLMDGL
                     DAGYQTALGWTFRHRAVVLGGIGVVLVSSLLAARWIGTEFFPSTDESQFEIDLEAPQG
                     MAVQVTSALAEQIAGIIRQVVPARDIITIYTNSGVPASGGGFGGNAGPNTASVQVRLV
                     PPTQRQRSTDALTDAVRKALQGKFPGVQLFARVGGLQNRVVNFGSAAPIDVQLLGYDQ
                     QVGAQFAQEVAGIVSSTPGTADTQITPRGQYPAFTVALDSEKAAQLGLSSTSVANSID
                     MAMSGNVGTASQFIDPVTGNEYNIVVQLMDQYRTHTEDLPNIPLAAVADPGPGSAPSN
                     APSVPIRVRDVAQITLGNQPLQITRKNEQRVIDV"
BASE COUNT          387 a          697 c          728 g          336 t
ORIGIN      
        1 acatcgatca cccgctgctc gttcttccgg gtgatctgga gcggctggtt tccgagcgtg
       61 atctgggcca cgtctcgaac ccggatcggg accgacggcg cattcgacgg cgccgatccc
      121 gggccggggt cggcgacggc ggcgagggga atgttgggca ggtcttcggt gtgcgtccgg
      181 tactggtcca tcaactggac gacgatattg tactcgttgc ccgtcaccgg gtcgatgaac
      241 tggctggcgg tgcccacgtt gccggacatc gccatgtcga tcgagttcgc gaccgaggtc
      301 gatgagaggc cgagctgggc cgccttctcg ctgtccaggg ccacggtgaa ggccgggtac
      361 tggccacgcg gggtaatctg ggtatccgcg gtgccgggcg tggacgagac gatccccgcg
      421 acttcctgcg caaactgcgc gccgacctgc tggtcgtacc ccaggagctg gacatcgatc
      481 ggggccgccg agccgaaatt gacgacgcga ttctggagcc cgccgacccg ggcgaagagt
      541 tggacgccgg ggaacttccc ttgaagcgcc ttccgaacgg catccgtaag ggcgtcggtc
      601 gatcgctggc gctgcgtcgg cggcaccaat cgaacctgga cgctcgcggt gttcgggcct
      661 gcgttaccgc caaaaccacc accggacgcg gggaccccgg agttcgtgta gatggtaatg
      721 atgtcgcgag cggggacgac ctggcgaatg atccccgcga tctgctcggc aagggccgag
      781 gtcacctgca ccgccattcc ctgcggcgct tcgagatcga tctcaaactg gctctcgtcc
      841 gtggacggga agaactcggt gccgatccaa cgggcggcga ggagggagga gacgagcacg
      901 accccgatcc cccccaggac gaccgctcgg tgccggaagg tccagccgag ggcggtctga
      961 tagcccgcat cgaggccgtc catcagtcct tcgctccacc ggttgaaccg tgccaccacc
     1021 ccgctgcctt ctgtgttcgc ggtgcccgcg cgcagcagct tgatgctcag cacaggatcg
     1081 atcgtcatcg acacgacgta ggaggcggcg agcgcgaaga cgatggtcag cgccataggc
     1141 acgaagagcc gctgcgcgat cccgctgagg aacacgacgg ggaagaacac ggcgatcgtc
     1201 gtcgccgtgg aggcaaggac ggggagtccg acttcctggg tggccgtgag caccgcctcg
     1261 agcacggacc gaccgggagt tgcgaggtga cgggtgatgt tctcccgcac cacgatcgcg
     1321 tcgtccacca accgccccat cgccagcgtg agcccaccga gcgtgaagac gttgagggtt
     1381 tggcccgtaa agtagagcag caacaacgcg ctggacaccg acaacgggat gcccagccca
     1441 acgatgaaca ggctccagag actccgcagg aacaccagga cgaccaggaa ggtcaagacc
     1501 gctcccagga tcgcctcgtt cccgagggtc ttgatcgcgg cccggatgta ccgggactgg
     1561 tcgaagccca cagacagggt caccccgggc ggcaaacccg tcaggctggg cagggccgcg
     1621 cggatcgcgt tgaccgtttg caccgtgttg gcgtccggct ggcgggcgac aaacatgacc
     1681 acgccgggct tgccgtccac attgacgatc tgggtctgat cggccgcggc gtccgacacc
     1741 cgcgcgacgt cccgaatgtg cacgggcacg cctccgcgcg tggccacgac gacgtcttgg
     1801 atcgccggga cgctctgcag cagcgaaggc acgttcagct ggtagtcgat ccggttgttg
     1861 cggaggctgc ccgacgggag aatctgattg tacctgacga tcgcgttctg cacatcctgc
     1921 agcgccaagc ccgccgcggc cagtttgtgg ggatccacgc tgacgctgag ctgccggacc
     1981 aacccaccgt tcacgaacgc cgtggaaaca ccaggcagcc gctcgagctg tggctcgatc
     2041 gtgttgtagg cgagatcgta caactgccgg gcgtcgagcc ccgcaccccg cacgacgacc
     2101 tgcgcgaccg ggatgttgga gacatcgaac ttcagcacga acggctgg
//
LOCUS       DASQGH010000025         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51032, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000025 DASQGH010000000
VERSION     DASQGH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51032"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VEW91_00420"
     CDS             <1..804
                     /locus_tag="VEW91_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HYM90079.1"
                     /translation="LVRGDPGPDPDQIPWDPAAGRLDGTRLEGTDAVIHLAGESIAAS
                     RWTAARKARIRQSRVESTRLLAETLARLHRKPAVLIAASAVGYYGDRGDEILVEDSAP
                     GSGFLATLCRDWEAAAAPARESGIRVVHLRTGMVLSQAGGALARILPIFRAGLGGRLG
                     TGRQFMSWIGLDDEVEAIHHALARDAVRGPVNLVSPQPVTNREFTTTLGRILRRPALL
                     AVPAGLLRVALGELGGEILTSVRAYPARLLATGYAFRHTDLESALRHLR"
     gene            863..1330
                     /locus_tag="VEW91_00425"
     CDS             863..1330
                     /locus_tag="VEW91_00425"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HYM90080.1"
                     /translation="MRIAIASDHAGYPLKEELKRLLRDWGHEVRDFGAHSTDTVDYPD
                     FIVPAAEAVASGQCDRGIVLGGSGNGEAIAANKIPGVRCTLCWEGYTARVAREHNDAN
                     VLSLGARAIGTEVAREIVRIWLETAFAGDRHQVRIDKIRAVEERYASPRAVKG"
     gene            complement(1331..>2258)
                     /locus_tag="VEW91_00430"
     CDS             complement(1331..>2258)
                     /locus_tag="VEW91_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90081.1"
                     /translation="MREGATAADLRPGTGTLTHPRARDVAWQRAGRRFFRNRLTTLGL
                     GIVVLFLLVAGFAPYLAPYPGDVTGAVHTAERLQPPGPAHALGTDEVGRDVLSRLLDG
                     TRVSLAIGAIIIAVAMAIGVPLGLAAGVMGGWVEEAIMRTTDVFLAVPGIVLAIAIVT
                     VLGPGIAHAMVAISLVWWPGYVRLVHGRATALRGSMFVEAARALGAPWRRVILRHVLP
                     NLLTVIVIKASMDMGMAILTAAGLGFVGVGAQPPIPEWGEMISAGQPYLPTWWWLSVF
                     PGAAIVVTVLGFNLLGDGLRDLLDPATRQAVA"
BASE COUNT          352 a          803 c          820 g          283 t
ORIGIN      
        1 ctggtgcgcg gggatcccgg ccccgatccg gaccagatcc cttgggatcc cgccgccggg
       61 aggctcgacg gcacccggct cgaaggcaca gacgccgtga tccacctggc cggcgagagc
      121 atcgcggcca gccggtggac ggccgcgcgg aaggcgcgga tccggcagag ccgcgtcgag
      181 agcacccggc tcctcgcaga aacccttgcc cgcctccacc gcaagcccgc ggtcttgatc
      241 gccgcctcgg ccgtcgggta ctacggggac cggggcgatg agatcctggt cgaggacagc
      301 gcgccgggga gcggctttct cgcgaccctc tgccgggatt gggaggcggc cgccgccccg
      361 gcccgagaga gcggcatccg ggtggtgcac ctgcggaccg ggatggtgct gagccaggcg
      421 ggaggggcgc tcgcaagaat tttgccgatc ttccgcgccg ggctgggggg ccggctgggg
      481 accgggcggc agttcatgag ctggatcggg ctcgatgatg aggtcgaggc catccaccat
      541 gcgctggccc gcgacgcggt ccggggcccg gtgaacctgg tgtcccccca gccggtgacg
      601 aaccgggagt tcaccacgac cctcggccgg attctccggc gtcccgcgct gcttgcggtg
      661 ccggctggat tgcttcgggt cgcgctgggc gaactcgggg gcgagatcct caccagcgtc
      721 cgcgcctacc ccgccagatt gctcgccacc gggtacgcgt tccgccacac cgacctggag
      781 agcgcgctgc gccatcttcg ctgacggccg ccgcgcggca acggcggcag gggcgcccgt
      841 gacgctgcgc gaattgagcg atatgcgcat cgccatcgcg agcgaccacg cgggctaccc
      901 cctgaaggag gaactcaagc gcctcctgcg cgactggggg cacgaggtgc gggatttcgg
      961 tgcgcactcg accgacacgg tggactatcc cgacttcatc gtgccggcgg ccgaggccgt
     1021 cgcctcgggg cagtgcgacc ggggcatcgt gctggggggc agtggcaacg gcgaagccat
     1081 cgccgcgaac aagatccccg gggtgcgctg cacgctatgc tgggagggct acacggcgcg
     1141 ggtcgcccgt gagcacaacg acgccaacgt cctctcgctg ggggcgcggg cgatcgggac
     1201 cgaggtggcc cgagagatag tgcgcatctg gctggagacg gccttcgcgg gcgaccgtca
     1261 ccaggtgcgg atcgacaaga tccgggcggt ggaagagcgg tacgcgagcc ctcgggcggt
     1321 gaaggggtaa ttacgccacg gcctggcggg tggccggatc cagcagatcc cgcagcccgt
     1381 cccccagcag gttgaagccc agcaccgtca cgacgatcgc tgcgccgggg aacaccgaca
     1441 gccaccacca ggtgggaagg tacggctgcc ccgcgctgat catctcgccc cactccggga
     1501 tgggaggctg ggcgccaacc ccgacgaacc ccaaccccgc cgcggtcagg atggccatcc
     1561 ccatgtccat cgaggctttg atcacgatga cggtcagcag gttggggagg acgtgccgga
     1621 ggatcacgcg gcgccaggga gcccccagcg cccgcgcggc ctccacgaac atcgacccgc
     1681 ggagcgccgt ggccctcccg tgcaccaggc gcacgtatcc gggccaccac acaagcgaga
     1741 tcgcgaccat ggcgtgggcg atccccggcc cgaggaccgt gacgatggcg atcgccagga
     1801 cgatcccggg gaccgcgagg aagacgtcgg tggtccgcat gatcgcctcc tcgacccagc
     1861 cgcccatgac gcccgcggcc agccccagcg gcactccaat cgccatcgcg accgcgatga
     1921 tgatggcccc gatcgccagc gacacccggg tgccgtcgag caggcggctg aggacgtcgc
     1981 ggcccacctc atccgttccc agcgcgtgcg cggggccggg aggctgcagg cgctccgcgg
     2041 tatggaccgc gccggtgaca tcccccgggt agggcgcgag gtacggcgcg aatcccgcca
     2101 ccagtaggaa cagcacgacg attcccagcc cgagcgtggt gagccggttt cggaagaacc
     2161 ggcgccccgc ccgctgccag gcgacatcgc gcgcacgcgg gtgggtgagg gttccggtcc
     2221 ccggccgcag atccgccgcg gtggcgccct cacgcata
//
LOCUS       DASQGH010000026         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11423, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000026 DASQGH010000000
VERSION     DASQGH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11423"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VEW91_00435"
     CDS             complement(<1..703)
                     /locus_tag="VEW91_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYM90082.1"
                     /translation="MVTLVSLPKPFRGHAEVIQRNAIRSWTRLRPACQIILLGDDDGT
                     AEMARECGAHHVPEVARNEFGTPLLDSAFAAAERVATGDLLCYLNADIILMSDFMATV
                     TRLAGQMRRFLMIGQRCNVHIAGPWDFDQPDWEHHLRSYARRHGTLSGLFYGADYFVF
                     TRGLWQTLPPLAIGRYFWDTWLIFRARSLGVPVFDATDCVVAVHQAHDYSHAPTVNAW
                     VSAPEARRNLTLSGGY"
     gene            complement(765..1730)
                     /locus_tag="VEW91_00440"
     CDS             complement(765..1730)
                     /locus_tag="VEW91_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYM90083.1"
                     /translation="MALSARLAVSVVVPCKNDAHYLSAALESILSQDYPLLECIVVDG
                     GSTDGTIELLKRYSERIRWLSEPDRGAFDAINRGWQLCHGEILAWLNADDLWDVGAVR
                     TVVDFFGRWPDVDVVYGMAGLVDERGQVYRDLMPRAWDLEYALRRCDHVIFQPASFMR
                     RRILERVGWLSPAWCHDHDLWLRIARAGGKFARLPLRLAMDRARPSNLGAQLEIAVPA
                     KVALTRRFFADPGLPPATRRLERRAISAAYALAVNYLRITNPRGWLLAARFLMQAIAV
                     DPGNTRFIGELFTRRVARGIKWLLSPVLRPRSRMVARRVRSSPRG"
     gene            complement(1837..2466)
                     /locus_tag="VEW91_00445"
     CDS             complement(1837..2466)
                     /locus_tag="VEW91_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90084.1"
                     /translation="MYRIDRLDVSPDMPFDASLDEQLEFCRRHLVRVNGRRRISRADE
                     IGLAGGLRRIRGRSPIASVGAAWRWARKRVTDRFMPLHTTACGDFTLLARDAWFRLRG
                     YPELQMYSLHHDSILCYTAHYSGVREVVLKGDMRMYHLDHSSGWSTELAQRMRSIGLP
                     FLDDEQFRSYIRVMHERRSPTIFNDESWGLATEDLVETDPVTRRAEAPR"
BASE COUNT          530 a          863 c          823 g          424 t
ORIGIN      
        1 cgtacccgcc ggacagcgtc aggtttcggc gggcctcggg ggccgacacc cacgcgttaa
       61 cggtcggagc atgcgagtag tcatgggcct ggtgaaccgc gacgacgcaa tcggtcgcat
      121 cgaagaccgg aacgccgagc gatcgcgccc gaaagatcaa ccaggtgtcc cagaaatacc
      181 gtccgatggc cagcggagga agcgtctgcc agagtccccg agtgaacacg aaatagtccg
      241 caccatagaa aaggccggaa agcgtgccat ggcggcgggc gtaggaccgc aggtggtgtt
      301 cccaatctgg ttggtcgaaa tcccagggcc cagcgatgtg gacgttgcac cgctgcccga
      361 tcatcagaaa ccgtcgcatc tggccggcga gccgggtgac cgtcgccatg aaatcactca
      421 tcaggatgat gtccgcattg agataacaga gcaggtcgcc ggtggcgacg cgctctgccg
      481 ccgcaaacgc cgagtcgagc aggggcgtcc cgaattcgtt gcgggccacc tccgggacgt
      541 gatgcgcgcc gcattcccgg gccatctcgg ccgtgccgtc gtcatctcct aagaggatga
      601 tctggcaggc cggccggagc ctcgtccagc tcctgatggc gttacgctga atgacctccg
      661 cgtggccccg aaacggcttg ggaagcgaga ccagggtcac catccgaggc ccccggacgc
      721 gcagggaatt tctggccctt ggcgccgatc ggtgtcgatc atcgtcatcc ccgtggtgag
      781 ctccggactc tccgcgccac catcctggag cggggcctca agacggggga gaggagccac
      841 ttgatgcccc gagcgacgcg ccgcgtgaac agctcgccga tgaagcgggt gttcccggga
      901 tccacggcga tcgcctgcat cagaaaacgc gcggccagca gccagccccg agggttcgtg
      961 attcggagat agttgacggc gagcgcatag gcggcactga tcgcacgacg ctctagacgc
     1021 ctggtggcgg gcggcaatcc gggatccgca aaaaaccgcc gggtcaacgc gaccttggcc
     1081 gggacggcga tctccagctg agcccccagg ttgctgggcc gcgcgcgatc catcgcgagg
     1141 cgaaggggga gccgagcgaa cttccctccg gctcgggcga tccgcaacca caggtcatga
     1201 tcgtgacacc aggcaggaga gagccacccg actcgctcga ggatgcggcg ccgcatgaag
     1261 gaggcgggtt ggaagatcac gtggtcgcag cggcgcagcg cgtattccaa atcccacgcg
     1321 cggggcatga gatcccgata cacctgtccg cgttcgtcga ccagcccggc catgccgtac
     1381 accacgtcca cgtccggcca gcgtccaaag aagtcgacga cagtgcggac ggcgccaacg
     1441 tcccagagat cgtcagcgtt cagccacgcg aggatctcac catggcagag ttgccagcct
     1501 cggttgatgg cgtcgaaggc gccgcgatca ggctcggaca gccagcgaat cctctcgctg
     1561 taccgcttga ggagttcgat cgtgccatcc gtagagccgc catccacgac gatgcactcg
     1621 agcagggggt agtcttggct caggatgctc tcgagggccg ctgaaaggta atgggcgtcg
     1681 ttcttgcacg gaacgacaac cgagaccgcc aatcgggccg agagtgccat ctagaaccaa
     1741 ccccctcgag cagtgggtgc cccaaacccc cgccacctag actccgcagg ctggcggttg
     1801 acaagacccg cgtcgctttg gttgcccgca gcttcatcac cgcggggctt ccgcacgtct
     1861 cgtcaccggg tctgtttcca ccaggtcctc cgtggcaagc ccccaactct cgtcgttgaa
     1921 gatcgtcggc gatctcctct catgcatgac cctgatgtac gaacggaact gctcatcatc
     1981 cagaaacggc aaccctatcg atcgcattcg ttgcgcgagc tccgtggacc atccggagga
     2041 gtggtccaga tgatacatgc gcatgtctcc cttgagcacg acctcccgca ccccgctata
     2101 gtgcgccgtg tagcagagaa tggaatcgtg gtgcaatgaa tacatctgca actcaggata
     2161 ccctctgagg cggaaccatg cgtcccgagc gagcagcgta aaatcgccgc aggcagtcgt
     2221 atgcagcggc atgaagcgat ccgtcacacg cttacgtgcc cagcgccatg ccgcaccaac
     2281 gctcgcaatg ggggagcgac cgcgaatcct gcgcagccct ccggctaagc caatttcatc
     2341 cgccctgctg attctccgcc ggccgttcac ccgaaccaga tggcggcggc aaaactcgag
     2401 ttgctcgtcc agcgaggcgt cgaacggcat gtcgggcgac acgtccagac gatcgatccg
     2461 atacatgcat cgggggtcga ggtgtccgga tgccaagaac ctcacgagct cccccgagaa
     2521 caagaggtcc gcgttcgtac acagcacgaa ccgcccgcgg gatcggcgaa tccccgcatt
     2581 cttcgcgatc atctgataga ggcccaactt gtctgcgtgc ttgtagcgac ggtggatcgc
//
LOCUS       DASQGH010000027         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_131, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000027 DASQGH010000000
VERSION     DASQGH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VEW91_00450"
     CDS             complement(<1..410)
                     /locus_tag="VEW91_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HYM90085.1"
                     /translation="MLKLFQSLLGDPSERIVKHLRESLVQEVNALEPEFEGLPEAALR
                     GKMDEFRERAKTEPLETLMPEMFALVREASKRALGQRHHDVQLIGGAVLFSGKIAEMK
                     TGEGKTLVATLPLALTALEGRGGHLVTPNDYLSR"
     gene            complement(469..1119)
                     /locus_tag="VEW91_00455"
     CDS             complement(469..1119)
                     /locus_tag="VEW91_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90086.1"
                     /translation="MERERRSLADEAEAVIRHLRGVSAARVELKDGQIDQVHVLGSAD
                     RTARVLVADVIAALAAELGVTLEPPQVRVALLRPGQSQPGPAPLRARLKVVGLTVTTL
                     RDSQEARVRIEHEGLAYEGAASGPAGVANSPQVVGAATLRAVETYLRSGVVFHLKAAA
                     VTPLGPHQVALALVGWAGPQEELLSGAALVRDDPRDAVVRAVLDAVNRPVGWLGAR"
     gene            complement(1175..1741)
                     /gene="raiA"
                     /locus_tag="VEW91_00460"
     CDS             complement(1175..1741)
                     /gene="raiA"
                     /locus_tag="VEW91_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYM90087.1"
                     /translation="MQIIVAGRNVEVSGALRARVTEKVGRLAHHFEQVQRAQVWLRVE
                     SHPGRNQVVEVTVWGDGVVLRGEEASPDMYASIDRVVDKLDKQISKFRSRVIKRRRLL
                     AGRRKQRAVVAAEAALRESPGEGETASVAIYRRKRFEMKPMTPEDAAVQMELLGHAFY
                     MFRNSETLEVNVVYRRADGRYGLIEPEG"
     gene            complement(1752..>2591)
                     /locus_tag="VEW91_00465"
     CDS             complement(1752..>2591)
                     /locus_tag="VEW91_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYM90088.1"
                     /translation="VSAGLPPTRSRPASAGPLHRPAPPLHAVLAASVFLLAACNSLGA
                     GRPPLPAPPHPAVQGPVVAAIRRAGTLRVASDLSYPPLAFRAGTSPGGFEIDLAGLLA
                     AALGVRLDVVDTPRAAMRPGFADADLLISQLTDAEAPGLPSPPYYVMRQAILWREGEK
                     PVPDGSLPGVRVAVQARSRGQAVAEQLGVAPAVVAYQPIEALRAVSRGQAQAAVADLP
                     LVVEYARTHRGLSVSTGRWPESPLVVVVRADAPDLLAFTSAAIRELEQTGGLTRLRQR
                     WHL"
BASE COUNT          390 a          961 c          871 g          369 t
ORIGIN      
        1 atccgggaca ggtagtcgtt gggggtcacg agatgtccgc cccgcccttc gagcgcggtc
       61 agcgcgagcg gcagcgtcgc gacgagcgtt ttcccctcgc ccgtcttcat ctcggcgatc
      121 tttccgctga agaggaccgc gcccccgatg agttggacgt cgtgatgccg ctggccgagg
      181 gcgcgtttgc tggcttcgcg gacgagggcg aacatctcgg gcatgagcgt ctccagcggc
      241 tcggtcttcg cccgctcgcg gaactcgtcc atcttcccgc ggagggccgc ttcggggagc
      301 ccctcgaact cgggctccag cgcgttcact tcctgaacca gcgactcccg caggtgcttg
      361 acgatccgct cgctcggatc gccgagcagc gattggaaca gcttcagcat cggttggctc
      421 acccaacgtc aattatagtc gatccccaca acccggtcgt gcgcggccct agcgggctcc
      481 cagccagccg acgggccggt tcaccgcatc gagcaccgcc cggaccaccg cgtctcgagg
      541 gtcgtcccgc acgagcgcgg caccgctgag gagctcctcc tgcgggcccg cccaccccac
      601 gagcgccagc gcgacctggt gggggccgag cggcgtcacg gcggccgcct tgaggtggaa
      661 caccactccg gatcggaggt aggtttcaac cgcccgcagg gtggcggccc cgacgacctg
      721 cgggctgttg gcaacgccgg cgggaccgga ggcggcaccc tcgtaggcca acccctcgtg
      781 ctcgatccgc acccgggcct cctgggaatc gcggagggtc gtcaccgtca gcccgaccac
      841 tttgagcctc gcgcgcaggg gggccggccc cggctggctc tggcccgggc ggaggagcgc
      901 gacccggacc tgcggcggtt ccagggtcac cccgagctcg gcagccaggg cggcaatgac
      961 atccgccacc aggacccgcg cggtccggtc ggcggacccc agcacgtgga cctggtcgat
     1021 ctgcccatct ttgagttcca cccgggcggc cgagacaccg cggagatgcc ggatgaccgc
     1081 ctcggcctca tccgccaggg accgacgttc tcgctccatc gcgcctccta gcctcgctcc
     1141 aacgtgatgc cccccatcac ctggtgggag gctattaccc ctccggctcg atcagcccgt
     1201 accggccatc ggcacgccgg tacacgacgt tcacttccag cgtctcggag ttgcggaaca
     1261 tgtagaacgc gtggccgagc agttccatct gcaccgccgc atcctccggc gtcatcggct
     1321 tcatctcgaa gcgctttcga cgatagatcg caaccgatgc cgtctccccc tccccggggc
     1381 tctcccgaag cgccgcctcg gcggcgacca ccgcccgctg cttgcgccgg cccgcgagca
     1441 ggcggcgccg cttgatcacc cggctgcgaa acttgctgat ctgcttgtcg agcttgtcca
     1501 ccacacggtc gatcgaggcg tacatatccg ggctcgcctc ctcgccccgg aggaccacgc
     1561 cgtcgcccca gacggtgacc tcgaccacct ggttccggcc ggggtgcgat tctacccgca
     1621 gccacacctg ggctcgctgg acctgctcga aatggtgggc caaccggccg accttctcgg
     1681 tcacccttgc ccgcagggcc ccgctgacct cgacgttccg acccgccacg atgatctgca
     1741 tacgacctcc tttacaggtg ccagcgttgg cgcaaccggg tcagacctcc cgtctgctcg
     1801 agctcacgga tcgccgcgga ggtgaacgcc aggaggtccg gcgcgtccgc gcggaccacc
     1861 accacgagag gggattcggg ccaccgcccg gtggacacgc tcaggccccg gtgggtccgc
     1921 gcgtactcca cgaccagcgg cagatcggcg acggcggcct gcgcctgccc gcgcgagacc
     1981 gccctcagag cttcgatcgg ctggtaagcg accaccgcag gggcgacacc cagttgctcc
     2041 gccaccgcct ggccgcggct tcgcgcctgc accgcaacgc ggacgcccgg gagggatcca
     2101 tcggggaccg gcttctcccc ctcacgccag aggatggcct gacgcatcac atagtagggg
     2161 ggtgagggca ggccgggggc ttctgcgtct gtcaactggc tgatcagcaa gtccgcgtcg
     2221 gcgaaccccg ggcgcatcgc cgctcggggg gtgtcgacga catcgaggcg gacgcccagg
     2281 gccgcagcga gcaggccggc aaggtcgatc tcaaaccctc cgggggaggt gcccgctcga
     2341 aacgccagcg gagggtagga gaggtcggac gcgacccgca gggtacccgc ccgcctgatg
     2401 gccgccacca ccggaccctg caccgcgggg tgcggcgggg ccggaagcgg agggcggccg
     2461 gcccccagcg agttgcaggc agcgagaagg aagaccgatg cggccagcac cgcatgaagc
     2521 ggtggggccg gtcggtgcaa cgggcccgcg gaggcaggcc gggatcgggt ggggggcaag
     2581 ccggccgaga c
//
LOCUS       DASQGH010000028         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8593, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000028 DASQGH010000000
VERSION     DASQGH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8593"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="VEW91_00470"
     CDS             complement(<1..2193)
                     /locus_tag="VEW91_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90089.1"
                     /translation="MASKIEERELVVVGGGPAGMAAAIEARRAGAQVTLVEERATLGG
                     QIYKQPPLGFEVKRREALGKEYRAGRELIQGALECGAEILTDHVVWSVWGRDTKEVSL
                     YQQGQQARTFLTTKVILATGAYDRPVPFPGWTLPGVISAGGAQSMVKVQKVYPGSRIL
                     LAGSGPLVLAFAVQLHQMGARVVAVLEAAPAPGPGAVARLLAAGAAGNLEPLVEGIAY
                     LAYLRRHGIPLLFGHAIARAEGEREVERAVAVRVDRTWRPIPGTERTFEVDTVCVGYG
                     FFPSVEIARTLGCALVYDEQSGGHVPRRSEDMETSVPGVFAAGDGSGVAGSAVALEEG
                     RIAGIAAARQLGKLSWEMAAKRLAASRRRLVALGRFRDTLNAIYQVGQGIYEWATVDT
                     IVCRCEDVTLGEVRRFIPPGSRDPNYVKSLTRVGMGLCQGRNCSRQVSYVFARETGRL
                     PELPPLSSRPPVKPVPIGLIADTSAQQAPNPCDYDLALAEPPRGAEPSSSVDETDVLI
                     IGGGIVGCACAYYLTKEKVEWLVVDRGELHREGSGTNPGSLHIQLRRPERLYSDEVLR
                     EFVPLKIEAARMWADIERELGCDLGVKLRGGFLVAETDEEYAFLREMVDFEGTLGLET
                     ALLSTGEMLRISPHMSDKLKGAAYNPTEGYANTLLVGPTYAHRAMQAGARFRTYTDVI
                     AIDCVDGGGFQVTTSTGKIRAKRILNAAGPWAGQVSAKVGVPLPVGGRV"
     gene            complement(2177..2539)
                     /locus_tag="VEW91_00475"
     CDS             complement(2177..2539)
                     /locus_tag="VEW91_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYM90090.1"
                     /translation="MESANEFVRRVGTHGAVARGSRISIEVDGEPLEAYVGETLAAAM
                     LAAGRRVFHHSLRTDSPRGMYCGMGVCFECLMVVNGQPNVRACMTPVLPGMRASTQHG
                     PVADKSPVPNWECDGQQD"
     gene            complement(2700..>2939)
                     /locus_tag="VEW91_00480"
     CDS             complement(2700..>2939)
                     /locus_tag="VEW91_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90091.1"
                     /translation="YHAFDQTEQVQRAPDAISGRLCTWVEVADGQFAEKSGREFSTED
                     PCISRGMTFGGNDRARRQAVEDVTAFLRQLFKLGQ"
BASE COUNT          497 a         1069 c          872 g          501 t
ORIGIN      
        1 gacccgcccc ccgaccggaa ggggcacgcc caccttggct gatacctggc ctgcccatgg
       61 cccggccgcg ttcaagatcc gcttcgcgcg gatcttcccg gtgctggtcg tcacctggaa
      121 cccgccgccg tccacgcaat caattgcaat gacgtcagtg taggtgcgga agcgagcgcc
      181 ggcctgcatt gcacggtgcg cgtaggtcgg gccgaccagc agggtgttgg cgtacccctc
      241 ggtaggattg tacgcggcgc ccttcagctt gtcggacata tggggggaga tacggagcat
      301 ctcgccggtc gacagcagcg ccgtttccag acccagcgtc ccctcgaagt cgaccatctc
      361 tcgcaggaac gcgtactcct cgtcggtttc cgccacgagg aaacctcctc gcagcttgac
      421 gcccaagtcg caccccaact cccgctcgat gtcggcccac atgcgggcgg cttctatctt
      481 gaggggaacg aactcacgga gcacctcgtc ggagtatagc cgctcaggcc ggcggagctg
      541 gatgtggagg ctgccggggt tggtgcccga cccctcgcgg tgcagctcac cgcgatccac
      601 caccagccac tccaccttct ccttcgtcag atagtaggcg caggcgcagc caacgattcc
      661 cccgccgatg atcagcacgt ccgtctcgtc gacactcgac gagggctcgg ctcctcgcgg
      721 gggttccgcc agggcgaggt cgtagtcgca cgggttaggc gcctgttgcg ccgaggtatc
      781 ggcgatcagg ccaatcggca ccggcttgac cggcggtcgg gacgacaggg gcgggagctc
      841 cggaagacgg cccgtttctc gggcgaaaac gtagctgacc tgccgcgagc agttgcgccc
      901 ctggcagagc cccatgccca cccgggtcag cgacttcacg tagttgggat cgcggcttcc
      961 cggcggaatg aaacggcgca cctcacccag ggtcacatcc tcgcaacgac atacgatggt
     1021 gtcgaccgtc gcccactcgt agatgccctg gcccacctga tatatggcat tcagcgtgtc
     1081 ccggaagcgt cccagggcca ccagccgccg ccgggacgcc gccagccgct tggccgccat
     1141 ctcccacgag agcttcccca gttgtctcgc ggccgctatc ccggcgatcc gaccctcctc
     1201 gagagccacc gccgatccgg ccaccccgga gccgtccccg gcggcgaaca ccccgggcac
     1261 gctggtctcc atatcctcgc tgcgcctggg gacgtggccg cccgactgtt catcgtagac
     1321 gagcgcgcat cccagggttc gcgcgatctc gaccgatggg aagaagccgt agccaacgca
     1381 caccgtgtcg acctcgaagg tgcgctccgt cccggggatc ggccgccagg tgcggtcgac
     1441 cctcaccgcg acggcccgct ccacctcacg ctcgccctcg gctcgagcga tggcgtgccc
     1501 aaacagcagc gggatgccgt gtcgccgcag gtaggccagg taagctatcc cctctaccag
     1561 cggctccagg ttccccgccg cgccggccgc gagcagcctg gccacggcac ccggccccgg
     1621 cgcaggggcc gcctccagca ccgccaccac cctggcaccc atttgatgca actgcaccgc
     1681 gaaggccagt accagggggc cgcttcccgc cagcaggatg cggctccccg ggtagacctt
     1741 ctgcaccttc accatggact gggctccgcc ggcgctgatc actcccggca gggtccaccc
     1801 tgggaacggt acgggccggt cataggcgcc ggtcgctagg atcaccttgg tcgttaggaa
     1861 ggtgcgcgcc tgctggcctt gctggtacag ggagacctcc ttggtatccc tcccccaaac
     1921 ggaccacacc acgtggtcgg taaggatctc ggcgccgcac tcgagcgcgc cttggatcaa
     1981 ctcccgacca gccctgtact ccttgcccag tgcctcgcgg cgcttcactt cgaaacctaa
     2041 gggtggctgc ttgtagatct gcccgccgag cgtcgccctc tcctcgacga gagtgacctg
     2101 agcacccgca cgccgcgcct cgatggctgc cgccattccc gcgggaccac cgccgacgac
     2161 caccaactca cgctcttcaa tcttgctggc catcgcactc ccagttcggc acggggctct
     2221 tgtccgcgac ggggccgtgc tgggtactgg cgcgcatgcc cggcagcact ggagtcatgc
     2281 acgctcgcac gttcggctgg ccgttcacca ccatcaagca ctcgaagcat acgcccatcc
     2341 cacagtacat tccgcgcggg ctatccgttc gcagcgaatg atggaaaacg cgacggccgg
     2401 cggctagcat ggcggccgcg agcgtttcgc ccacgtaggc ctccagcggt tcgccgtcca
     2461 cctcgatgga gatgcgcgac ccccgcgcga cggcgccatg ggtacctacc cgtcgcacga
     2521 actcgttcgc ggattccatt ctaacgcgcg ccctgatcca aaaacgttgc cccgaccatg
     2581 tgcttgtcgc agatattacc ggaatttccg ttttggattt tcgactcatc ttcagtgtgc
     2641 acctgcacac cttgcccggg gtgaaagatc ccagcaatcc gtacaagaag acgaggagcc
     2701 tactgcccca acttgaacag ttggcggaga aacgctgtta cgtcctccac tgcctgccgt
     2761 cgggcgcgat cattgccgcc aaacgtcatc ccgcgtgaga tgcaaggatc ctctgtgcta
     2821 aattcacgac cggacttctc ggcaaactgt ccgtcggcca cctcaaccca ggtacagagt
     2881 cgtccactga ttgcgtcggg ggcccgttga acttgttccg tctgatcgaa agcgtgata
//
LOCUS       DASQGH010000029         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11438, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000029 DASQGH010000000
VERSION     DASQGH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11438"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VEW91_00485"
     CDS             <1..911
                     /locus_tag="VEW91_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYM90092.1"
                     /translation="EETFRWYAKGQVVWSDPPVMALHVRDPGAMYRLKGVYLPPLGVA
                     GFRVTGFTTREGGGGSGAPDNTRFVILSDPSTGHPLAIVDEHWNYSVRTVAAAVVAAK
                     YLADPGAAELGLVGAGQLATTGLRALKQCFPLRKVRVYSRRWESRSAFAQRMSQDLGI
                     SVTAVDSAEAAVKDAPLILACTSAGRPLIQAAWIAPGAFLCTLGRDELDPSCYVQADK
                     IVLDSWELSQESSDVQKLIRSGVLSRDRVYAEIGEIVTGSKPGRQGRERVVVRAEGMG
                     NQDIAIAAWTYRQAAKSGIGMVLPVG"
     gene            1005..1415
                     /locus_tag="VEW91_00490"
     CDS             1005..1415
                     /locus_tag="VEW91_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HYM90093.1"
                     /translation="MEIQIVLAGVTGRITLLDAAAPRTCHALLAKLPIADHAVQVKWS
                     GDAWRTEGDYDIGVAEAENEGHVLVAGDFIYYPRMRKIGLAYGRAEWRHPDISFKLHV
                     SVIGKVSAQLEQLVTASQRVWLEGRQSFRLDRLG"
BASE COUNT          250 a          534 c          570 g          283 t
ORIGIN      
        1 tcgaggagac atttcgctgg tacgcgaagg ggcaggtggt gtggtccgac cctccggtga
       61 tggcgctcca tgttcgggac ccaggcgcga tgtaccgtct caagggggtc tacctcccgc
      121 ccctcggcgt ggccgggttt cgcgtgaccg gcttcacgac ccgcgagggc ggtggtggtt
      181 ccggcgctcc cgacaacacg cgcttcgtca tcctgagcga tccctctacc ggacatccgc
      241 tggcgatcgt cgacgagcac tggaactact ccgttcgcac ggtggcggcg gccgttgtgg
      301 ccgcgaagta cctggccgac cctggggccg cggagctcgg cctcgtcgga gcgggccaac
      361 tggccacgac cggcctgcgg gcgctcaagc agtgcttccc gctgcgaaag gtccgcgtct
      421 actcgcgccg gtgggagagc cgcagcgcgt tcgctcagcg catgagccag gatctcggaa
      481 tcagcgtgac tgccgtggac tcggcggagg cggccgtcaa ggacgcccct ctcattctgg
      541 cgtgcacgtc cgcggggcgc cccctgatcc aggccgcgtg gattgccccg ggcgccttct
      601 tgtgcacgct gggacgggac gagctggatc cttcctgcta cgttcaggcc gataagatcg
      661 tgctcgacag ctgggagctc tcccaggaat cctcggacgt ccagaagctc atccggtccg
      721 gcgtgctcag ccgcgaccgt gtctatgcgg agatcggcga gatcgtcacg ggctccaagc
      781 ccggccgcca ggggcgtgag cgcgtcgttg tgcgggcgga ggggatgggt aatcaggaca
      841 tcgccatcgc cgcctggacc taccggcagg cagccaagag cggcatcggg atggtgctcc
      901 ccgtcggtta acagtgccac ggcacccttg ttgtggagac cgcaccgcag acgaccggcg
      961 cgatgtggca atcggtgccg tgcgagggtc gagggggact agccgtggag atccaaatcg
     1021 tcctcgcagg cgtcacgggc cggatcacgc tgctcgacgc ggcggccccg cgcacctgcc
     1081 acgcgcttct ggcgaagctg ccgatcgcag accacgccgt gcaggtgaag tggtccggtg
     1141 acgcatggcg taccgaaggt gactatgaca tcggcgtcgc ggaggccgag aacgaggggc
     1201 atgttctggt ggccggcgat ttcatttact acccgcggat gaggaagatc ggcctggcgt
     1261 atggccgcgc tgagtggcgg catccggata tttccttcaa gctccacgtg tccgtgatcg
     1321 ggaaggtcag cgcccagctt gagcagctcg ttacagccag ccagagggtc tggctggagg
     1381 ggaggcagtc attccgcctc gaccgcctcg gctgagctgc gggagcgtct ggatacgatc
     1441 cgggagatga cggaggttcg gctcggcagc gtctcctcct cgcgagggga cgctgccgag
     1501 atatcaccgg gctacgctct gctgacccgc atgggtcggc tcccttccca ctcgatcttc
     1561 ttcgcaaact ccgccaggct tgtcaggtcc ccttcgactc tccccacgcg gggaaccgga
     1621 tgggcgccgg cagcccc
//
LOCUS       DASQGH010000030         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19895, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000030 DASQGH010000000
VERSION     DASQGH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19895"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2010
                     /gene="rpoB"
                     /locus_tag="VEW91_00495"
     CDS             <1..>2010
                     /gene="rpoB"
                     /locus_tag="VEW91_00495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYM90094.1"
                     /translation="RTLLNTLFFDPRRYDLGRVGRYKLDKKLGFKPTPEGTPDARVIK
                     PKDIVEIVRYLVALINGEGRPDDIDHLGNRRVRSVGELLQNQFRIGMLRMERVIRERM
                     TIQDAGAITPQVLINIRPVVAAIKEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGG
                     LSRERAGFEVRDVHTSHYGRMCPIETPEGPNIGLISSLATFARVNPYGFIETPYRRVK
                     EGRVSRTIEYLTADDEERHTVAQANAKINQDGRLTESRVSARRGAVNVLVSPDKVDYM
                     DVSPKQIVSVATALIPFLEHDDSNRALMGSNMQRQAVPLLKTEAPLVGTGMEYRAAID
                     SGAVVVAREPGEVLSVAGEAIVVKPDRGKERTYKLVKFQRSNQGTCINQRPIVEVGER
                     VDVGDVIADGPCTDQGELALGHNVIVAFMPWEGYNYEDAILISERLLQKDFFTSIHIE
                     EYEVEARDTKLGPEEITRDIPNVGEEALRDLDEQGIVRIGAEVRSGDILVGKVTPKGE
                     TELTAEERLLRAIFGEKAREVRDTSLKVPHGEKGKVVAVKVFTREAGDELSPGVNELV
                     RVYVAQKRKITVGDKMAGRHGNKGVISKILPQEDMPFLPDGSAVDIVLNPLGVPSRMN
                     VGQVLETHLGWAAALLGFHAETPVFDGPREDEIRELLKKASKTEVG"
BASE COUNT          364 a          664 c          714 g          268 t
ORIGIN      
        1 cggaccctgc tgaacacgct cttcttcgac ccccgccggt acgacctcgg ccgggtgggc
       61 cgctacaagc tcgacaagaa gctcgggttc aagccgacgc ccgagggcac ccccgatgcg
      121 cgggtgatca agccgaagga catcgtcgag atcgtccggt acctcgtcgc gctcatcaac
      181 ggggagggcc ggcccgacga catcgatcac ctggggaacc ggcgggtccg ctcggtgggg
      241 gagctgctgc aaaaccagtt ccggatcggg atgctgcgga tggagcgcgt gattcgcgag
      301 cggatgacga tccaggacgc cggcgcgatc accccgcagg tgctgatcaa catccgcccg
      361 gtcgtcgccg cgatcaagga gttctttggc tcgagccagc tgtcccagtt catggaccag
      421 accaaccccc tcgcggagct gacccacaag cgccgcctct cggccctcgg gcccgggggg
      481 ttgagccgcg agcgcgcggg cttcgaggtc cgcgacgtcc acacctcgca ctacggccgg
      541 atgtgcccga tcgagacgcc ggaagggccg aacatcggcc tgatctcgtc gctcgcgacg
      601 ttcgcccggg tgaaccccta cgggttcatc gagaccccgt accggcgcgt gaaggagggc
      661 cgggtcagcc ggacgatcga gtacctgacc gccgacgacg aggagcgcca cacggtcgcg
      721 caggccaacg cgaagatcaa ccaggacggc cggctgaccg agtcccgcgt gagcgcccgg
      781 cggggcgccg tgaacgtgct cgtgtcgccg gacaaggtcg actacatgga cgtctcgccc
      841 aagcagatcg tcagcgtggc gacggcgttg atcccgttcc tcgagcacga cgactccaac
      901 cgggcgctga tgggctccaa catgcagcgg caggccgtcc ccctcttgaa gaccgaggcg
      961 ccgctggtgg gcaccggcat ggagtaccgc gccgccatcg attcgggcgc ggtcgtggtg
     1021 gcgcgcgagc ccggcgaggt gctgagcgtg gccggggagg cgatcgtggt caagcccgat
     1081 cgcggcaagg agcggacgta caaactcgtc aagtttcagc ggagcaacca gggcacgtgc
     1141 atcaaccagc gccccatcgt cgaggtgggc gagcgcgtgg acgtgggcga tgtgatcgcc
     1201 gacggcccgt gcacggacca gggcgagctc gcgctgggcc acaacgtcat cgtcgcgttc
     1261 atgccctggg agggctacaa ctacgaggac gcgatcctga tcagcgagcg gctgttgcag
     1321 aaggatttct tcaccagcat ccatatcgaa gagtacgagg tcgaggcgcg ggacaccaaa
     1381 ctcggccccg aggagatcac ccgggacatc cccaacgtcg gggaggaggc cctccgggac
     1441 ctcgacgagc aggggatcgt gcgcatcggc gccgaggtcc gcagcgggga catcctggtc
     1501 ggcaaggtga cgccgaaggg cgagaccgag ctcaccgcgg aggagcggct cctccgggcg
     1561 atcttcgggg agaaggcccg ggaggtccgg gacacctccc tcaaggtccc ccacggggag
     1621 aagggcaagg tcgtcgccgt caaggtgttc acccgcgagg cgggggacga gctcagcccg
     1681 ggcgtcaacg agctcgtccg ggtgtacgtg gcccagaagc gcaagatcac cgtgggcgac
     1741 aagatggccg ggcgccacgg gaacaagggc gtgatcagca agatcctccc gcaggaggac
     1801 atgccgttcc tgcccgacgg gagcgcggtg gatatcgtgc tcaacccgct cggcgtgccg
     1861 tcccggatga acgtcggcca ggtgctggag acccacctcg gctgggcggc ggccctcctc
     1921 gggttccacg cggagacccc ggtgttcgac gggccgcgcg aggacgagat ccgcgagctg
     1981 ctcaagaaag cgtccaagac cgaggtcggc
//
LOCUS       DASQGH010000031         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2914, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000031 DASQGH010000000
VERSION     DASQGH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VEW91_00500"
     CDS             <1..236
                     /locus_tag="VEW91_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90095.1"
                     /translation="GMGSTGGMDSGVHLHIVLDGATLMPSKDQLTAGKPHQYKYTLPT
                     LKAGPHTMKVYWADNKTHDARGPIHAAAFTCSE"
     gene            354..1208
                     /locus_tag="VEW91_00505"
     CDS             354..1208
                     /locus_tag="VEW91_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYM90096.1"
                     /translation="MVVRSLTVLLPALVVFGAEVVRHEWLHDVLPEMTGNVVTGVIAL
                     GVSTLVLIPIYRRLDHADARLRTMEIEHAVTEERERIARDLHDGISQALFFLNVKAAA
                     LAQSLRAPESRGTSQQLVGEISQAIEQTAGRARDAIFDLRTGPEPGQPFAGWVRAYAN
                     RFGEIHGLEATVEEIGRSIELPLDCELHAMAIVREALHNTAKHARARAVKIRIEWRAA
                     GATIAVEDDGAGLPDPVPGPPQGRYGLAALQEHAQAAGGQILVSGASGRGTSVVVRIP
                     YPTAETSG"
     gene            1205..1885
                     /locus_tag="VEW91_00510"
     CDS             1205..1885
                     /locus_tag="VEW91_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM90097.1"
                     /translation="MSRTRTLIVDDSELARRGIRAILKESPLFEVVGEANDGAMGIAR
                     VRELAPDLVLMDLRMPGLSGLEATRTIKEEFPATLVVVLSVSDDPQDLFEAIRCGAQG
                     YLIKPVGPGLWLEYLTAVVQGDARISRAVAERILEEFTRQSSVPRVALPTLTPREEEV
                     LALVARGMANKEIAATLAIVEGTVKRHLQNILAKLHLRNRVELAAFAITQGYAAPASL
                     RQRRPRQP"
     gene            complement(1900..>2506)
                     /locus_tag="VEW91_00515"
     CDS             complement(1900..>2506)
                     /locus_tag="VEW91_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90098.1"
                     /translation="FSMPGDAHERRTVGKFVMGVGWADEPAYAGFKNGVQLLLRDASD
                     KPVTDLPEDLKVEVIFGTQKFGPLALEAAFGKRFGTPGDFRAPLVPTRPGNYTLHFMG
                     SLDGQRIDESFTSSDKTFDPVVEASGIEFPAKDPSAGELGGLLERLSARVESVQASAR
                     DAAAAARAARTLGAIGIAAGIAGLIVALRLSRRRASGPSAP"
BASE COUNT          421 a          840 c          856 g          389 t
ORIGIN      
        1 gtgggatggg atcgacggga gggatggact caggggtgca cttgcacatc gtattggacg
       61 gggctacgct gatgccgagc aaggaccaat tgaccgccgg caagccccac cagtacaagt
      121 ataccttgcc caccttgaag gcaggcccgc ataccatgaa ggtctactgg gccgacaaca
      181 agacccacga cgcccgaggg ccgattcatg cggcggcgtt tacctgcagc gaatagcctc
      241 acgagacgcg ggcggccggt cgaccatcgc cgcggcggcg cgccggattg acgcccacac
      301 ctgccgcggc tcgaggacgt tcgcagggcc cccccacgtt gcggacgtcg cgtttggtcg
      361 tccggtccct caccgttttg ctgccggcgc tcgtcgtgtt cggggccgag gtggtccggc
      421 acgagtggct gcatgacgtc ctcccggaga tgaccggcaa cgtcgtgacc ggggtgattg
      481 cgctcggggt ctcgacgctc gtgctgatcc cgatctatcg ccgtctcgac cacgcggacg
      541 cgcgtctccg aacgatggag atcgagcacg ccgtcacaga agaacgcgag cgcatcgctc
      601 gcgatcttca cgatggcatc tcgcaggcgc tattctttct caacgtcaag gccgcggcgc
      661 tggcgcaatc cctgcgcgcc cccgagtcgc ggggcacctc ccagcaactg gtcggggaga
      721 tctcgcaggc gatcgagcag accgccggcc gggcgcggga tgcgatcttc gacctgcgga
      781 cgggaccgga gcccggacaa ccgttcgccg ggtgggttcg cgcgtacgcg aaccggtttg
      841 gcgagattca cggcctggag gccaccgtcg aagaaatcgg gaggtccatc gaactccccc
      901 tcgattgcga actgcatgcg atggcgatcg tgcgcgaggc gctccacaac accgccaagc
      961 acgcgcgggc gcgtgcggtg aagatccgga tcgagtggcg cgccgcgggc gccaccatcg
     1021 cggtggaaga cgatggtgcc ggattgccgg atcctgtgcc gggaccgccg cagggccgct
     1081 atggcctggc agccttgcag gagcatgccc aagcggcggg aggccagatt ctggtgtccg
     1141 gtgcgtccgg gcgggggacg tcggttgtcg ttcggatccc ctaccccacc gccgaaacct
     1201 ccggatgagc agaacgcgca cgttgatcgt ggacgacagc gagctggcgc ggcgcggcat
     1261 ccgcgcgatc ctgaaggaat cgcccctgtt cgaggtggtg ggggaggcca acgatggggc
     1321 catggggatc gccagggtcc gcgagctcgc gccggacctc gtcctcatgg acctgcggat
     1381 gcccgggctc agcgggctcg aagccacgcg caccatcaaa gaggaattcc cggcaacgct
     1441 ggtggtcgtg ctgagcgtat ccgacgatcc gcaagacctg ttcgaagcca tcaggtgtgg
     1501 cgcccagggc tacctcatca agcccgtggg gccgggcctg tggctcgagt acctgacggc
     1561 cgtggtgcaa ggcgacgcgc gcatttcgcg ggcggtcgcc gagcggatcc tggaggagtt
     1621 cacgcgccaa agcagcgttc ctcgcgtcgc cctgcccacg ctgacgccgc gcgaggagga
     1681 agtgctggcc cttgtcgccc gcgggatggc gaacaaggaa attgctgcca cgctggcgat
     1741 cgtcgagggc acggtcaagc gacatctgca aaacatcctc gccaagctgc acctgcgcaa
     1801 ccgtgtggag ctggcggcct ttgcgatcac gcaggggtac gctgccccgg cctccctgag
     1861 gcagcgtcgc ccgagacaac cgtaacaggt cccgcgcggt cacggcgcgc tcggccccga
     1921 tgcccggcgg cgactcagcc tgagcgccac gatcaggccg gcgatcccgg ccgcgatccc
     1981 aatggcgccc agggtcctcg cggccctcgc tgcggccgcg gcatcacggg ccgaggcctg
     2041 caccgactcc acccgggcgc tgagccgctc gagtagtccc cccagttcgc cggccgacgg
     2101 gtccttggcg ggaaactcga ttccactggc ttcgacgaca gggtcgaagg tcttgtctga
     2161 actcgtgaac gattcgtcaa tccgctgccc gtcgagtgac cccatgaagt ggagggtgta
     2221 gtttccggga cgcgtcggga ccaggggggc gcggaaatcc cccggggtgc caaagcgctt
     2281 cccgaacgcc gcttcgagcg ccaacggacc gaacttctgg gtgccaaaga tgacctcgac
     2341 cttgaggtcc tcgggaaggt cggtcacggg cttgtcggag gcatccctga gcaacagttg
     2401 cacgccgttc ttgaagcccg cgtacgccgg ttcgtcggcc caccccacgc ccatgacgaa
     2461 cttcccgact gttcggcgct cgtgcgcatc accgggcatc gagaac
//
LOCUS       DASQGH010000032         8928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53896, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000032 DASQGH010000000
VERSION     DASQGH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53896"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEW91_00520"
     CDS             complement(<1..447)
                     /locus_tag="VEW91_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90099.1"
                     /translation="MRAAAAAACLAAAGLGSHPGSPAAAAAQPAARPPLEVTGATRIE
                     YDDAARRWTFTGPRVAIVRASVRVEAPEIVYSERAREVVLPQGGAVLTPTLEVTADRI
                     ATLLDARHVTAEGHVAGRFAEEAPEKRWGTFSADRVEADDRPDLRQI"
     gene            complement(455..1093)
                     /gene="lptC"
                     /locus_tag="VEW91_00525"
     CDS             complement(455..1093)
                     /gene="lptC"
                     /locus_tag="VEW91_00525"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HYM90100.1"
                     /translation="MGIEAAWAVVRKVLLWAVPAVLVAALVWTFLPHGGTPPSPQAPS
                     THAAAPPGQSPADAGSEAAPPAQESGIEPYAKIRQGDLEGTDPDGGKRWRIVADDVTV
                     VQNRQVVLLRNVRAVFYAKDASTITVTGNRGQYDMRTREVEIDGSVHGVSSTGRELFA
                     DRLHWVPGAGTLTGTGHIRLLQERTIMYADRMVSSTTLGQTQFFGHVHASVR"
     gene            complement(1098..2273)
                     /gene="lpxB"
                     /locus_tag="VEW91_00530"
     CDS             complement(1098..2273)
                     /gene="lpxB"
                     /locus_tag="VEW91_00530"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HYM90101.1"
                     /translation="MAGASPRLLIVAGEVSGDHQGALLARALRRRRPDLLLAGVGGAE
                     MAAAGVDVLVESLRWGVIGYVEAYVRLPVFALRVWGLVRLIERYRPDLLVLVDFPGMN
                     RELVRHFSGRLPLVYFCPPQIHFRRGHPAGRVAQSAVRLLAILPFEADAYRSAGADVV
                     FVGHPAVDTVEGTAASVERLRGEWGLSGEPVIGLLPGSRVQEIRALLAPMLAAARELR
                     GGRTVQFLLPVASPFLRPRIHRALAGVSFPVRLVEGRALDVMRVSEVIVTASGTASVE
                     AACIGVPMVVVYRLSWLTDWIARRFVVTPEVYRVGFSIPNIVMQRRIVPELLNEEVRG
                     SRIRAEVERLLADPEARAQMQGDLAEVRRRLGPPGVLERAAEETLRALDSPRQATLR"
     gene            complement(2277..3104)
                     /gene="lpxI"
                     /locus_tag="VEW91_00535"
     CDS             complement(2277..3104)
                     /gene="lpxI"
                     /locus_tag="VEW91_00535"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HYM90102.1"
                     /translation="MTGPEAPIGLIAGRGALPVEVVAEARRRGRRVVCVDIFEGDPQL
                     PRVTDAYYTVTLGNLGGIIEAFRRHGVREVVLAGKVDKLPALRRAQLDAYGESVARRT
                     SDYRDTSIVGAFLAVLETSGFQVGDQARYLGHLIPGPGVLTPRAPSSAEAADIQVGLR
                     IAAQIAALDIGQAVAVRNGAVIAVEAAEGTDEMIRRVGALSAGAVVVKVSRPQQDPRY
                     DLPVVGPATIAALAASRGTALAVEAGRTLLVEREQIVSAAAASGIAIVAAAVQPAGA"
     gene            complement(3101..3922)
                     /gene="lpxA"
                     /locus_tag="VEW91_00540"
     CDS             complement(3101..3922)
                     /gene="lpxA"
                     /locus_tag="VEW91_00540"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HYM90103.1"
                     /translation="MTRAGRSRVPDQVSPLVHPSATVDPGAELEEGVRVGPHAVIESH
                     TRIGAGTWIGPLAVIEEWTTLGRDCRVHPAAVLGGVPQDRHFQGERSFLRIGDRVTIR
                     EYVSVSRATGEDAATVVGSDTQMLAYSHAGHNCQIGSSVIITNFTQLSGHVVVEDRVV
                     FSGMAGVIQFARIGTLAMVTGLARILKDVPPYMLVEGDPLRIVTINRVGLQRAGVPPE
                     VQLKLRRAHRLLTRSRLDVSHALERIEQELGDCREVQHLVEFIRESQARGMGIGR"
     gene            complement(3919..4404)
                     /gene="fabZ"
                     /locus_tag="VEW91_00545"
     CDS             complement(3919..4404)
                     /gene="fabZ"
                     /locus_tag="VEW91_00545"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HYM90104.1"
                     /translation="MDIKEIMRHIPHRYPLILVDRILELDPGRRIVGLKNVSANEPFF
                     VGHFPGHPVMPAVLIVEAMAQVGGVLASLLPGAEHKLAYFAALDRCRFRRPVRPGDQL
                     ITEVVVIRVRGRIGKMRVTSRVDGEVVADGVITYSMVDLEAVSQSVVDGQIVDLEEVE
                     P"
     gene            complement(4407..5234)
                     /gene="lpxC"
                     /locus_tag="VEW91_00550"
     CDS             complement(4407..5234)
                     /gene="lpxC"
                     /locus_tag="VEW91_00550"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HYM90105.1"
                     /translation="MDRSRQGTIGATIRLEGIGLHTGRHQRMTLHPAPPNTGILFRRA
                     TQGSAPIRATLDAVSGTTGRVSLGGEGGVQTVEHLLSAAWAAGVDNLDVELDGSEVPG
                     MDGSALPIFQALRGAGVRPQDAVRPVLEVREPVWVGNGQAWVVALPAPRFGVACVVTL
                     GAPAKEDQAATYDPAADRYEEVIAPARTWGYEREAETLRREGLALGASLENTLVIGGA
                     GYLGPRRFPNEPARHKILDLLGDLALLGRDIHGHIIAVRAGHGLHVALARALRDREG"
     gene            complement(5242..6258)
                     /gene="lpxD"
                     /locus_tag="VEW91_00555"
     CDS             complement(5242..6258)
                     /gene="lpxD"
                     /locus_tag="VEW91_00555"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HYM90106.1"
                     /translation="MRLDALAQRVGGDLVGAPSIEVQQVVPPEEARPGTVVVLADPRR
                     LPEVEAARAPVILSHDAPETHLPTIRVGNIRLALALAIRAFVPPKTPPSGIHPTCVIG
                     SRARIGDGAFLGPLVTVGDDVTIGARAQIHAHGVVEDGVQIGPECVLHARVTIRHGCS
                     LGARVVIQSGTVIGSDGFGYAQDAQRRHVLIPQVGRVVIDDDVEIGANVTVDRGTLGA
                     TRIGRGTKLDNLIHIAHNVQIGEDVAIAAAVFIAGSTRIGNRVLISGLTGITDHIEIG
                     DDAIVMGDTAVAQDVPPGEMVSGHPAGPRRSQRRAEVAYRRLPELVRRLRELEQRVRR
                     LEGA"
     gene            complement(6274..6678)
                     /locus_tag="VEW91_00560"
     CDS             complement(6274..6678)
                     /locus_tag="VEW91_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HYM90107.1"
                     /translation="MTWILVGLLALGAAFSVGRCGAAPVGIVDTQRVLNESVKALQYQ
                     KLLDNREKQMVAELAAVATQITPADLSGRRARYLTELGQMKRDLEGQLTQELGQVAGE
                     IAREDHLKLVLVKAPLWFGGRDITQQVIDRLK"
     gene            complement(6691..7842)
                     /locus_tag="VEW91_00565"
     CDS             complement(6691..7842)
                     /locus_tag="VEW91_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90108.1"
                     /translation="MSRAFVCAALAALVVGCGGRTPAAHPLSSSPASEASPQAGPRAL
                     RIAVVDLARAARVHPRWPEVTVLDRQVSELQAKLALETSGAAGRIEPPKIDLTPEMQA
                     AVERMQPEFQREAEAVKAAARQDMDAYVAQLRVEDQKKAEAKRAELEAELTKAVRDKQ
                     QALEADTQQFQQQTMGEYRLPLLNLKLKLETLQPAGKGESDRLTQQIQALTNERDEKI
                     AAHEKANRQALEDFQKERVQASSAELQAYQAQLAKEGQQLVNERAAKVTEQVRAQLTA
                     KQAEFNQRLRGQEQAIVSAARGTQAREAEARYRALVEAETARIRALQEELRGVQLGRA
                     RVFAVILADLRVEAAALAQEKGWDVVLTQAIVAPGTVDATDELIARIRR"
     gene            complement(7853..8302)
                     /locus_tag="VEW91_00570"
     CDS             complement(7853..8302)
                     /locus_tag="VEW91_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HYM90109.1"
                     /translation="MRWNAKTISIVAAAIVVVAAVGFVVARSAPALGQSVTIGYVDMP
                     RAIEAHPRKASAEGALKDYAQTQIADAQQRMKGMAAGQREEVQREVNAQLLKKRQELI
                     GGLEKDIRAAIEKIARDQGISIVLSREVVLYGGVDLTDQVVKAISAK"
     gene            complement(8335..>8928)
                     /locus_tag="VEW91_00575"
     CDS             complement(8335..>8928)
                     /locus_tag="VEW91_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYM90110.1"
                     /translation="PPGFNFAAFATPGQVNALILGVSQDTRNDPQTPISGEHITLGGE
                     FALLALGGTFQFQKYDFDYAHFFPLGGDSTIVGHAHLGYSAVPLPLQEQYYLGGQSTL
                     RGYPTGFLRGDDVVLGQVEYRFPLSWLPFMQSFTGITTVLFIDAGDAEPAGTSTFNLQ
                     PDVGIGIQVKTPVGPFRIDYGISGFGQQFWVSAGAQF"
BASE COUNT         1266 a         3341 c         2997 g         1324 t
ORIGIN      
        1 gatctgccgg aggtccgggc ggtcgtcggc ctccacccgg tcggcgctga acgtccccca
       61 ccgtttctcc ggcgcctcct cggcgaaccg ccccgcgacg tgtccctccg ccgtgacgtg
      121 ccgggcatcg agcagcgtcg cgatccggtc cgccgtcacc tcgagggtgg gggtgagcac
      181 cgccccgccc tgggggagca cgacctcacg ggcgcgctcg ctgtagacga tctcgggggc
      241 ctcgacccgg acgctcgcgc ggacgatcgc gacccgcggt ccggtgaacg tccagcgtct
      301 cgcggcgtcg tcatactcga tccgcgtcgc cccggtcacc tcgagcggcg gccgggcagc
      361 gggttgggcg gccgccgccg ccggggatcc cggatggctc cccagccccg ccgcggcgag
      421 gcatgcggcc gcggcagcgg cccgcacccg gcccctaccg gacggacgcg tggacgtgac
      481 caaagaactg ggtctgaccg agggtcgtgc tgctgaccat ccggtcggca tacatgatgg
      541 tgcgttcctg caggagcctg atgtggccgg tcccggtgag ggtgccggcc cccggcaccc
      601 agtgcagccg gtcggcgaac aactcgcgcc cggtgctgct caccccgtgc acgctgccgt
      661 cgatctccac ctcccgggtc ctcatgtcgt actggccgcg gttccccgtg acggtgatgg
      721 tgctggcgtc tttcgcatag aagaccgccc gcacattccg caggagcacc acctgtctat
      781 tctgcaccac cgtgacgtcg tccgcgacga tgcgccaccg cttcccccca tcggggtccg
      841 tcccctcgag gtcgccctgg cggatcttgg cgtatggctc gatgccggac tcctgcgcgg
      901 gaggggccgc ctcggatccc gcatccgcgg gggattggcc cgggggcgcg gccgcgtgcg
      961 tggagggcgc ttgcggggac ggcggcgtgc cgccgtgggg cagaaaggtc cagacgaggg
     1021 ctgcgaccag gaccgcggga accgcccaga gcagcacctt gcgtacgacg gcccacgccg
     1081 cctcgatgcc cacgacccta tcgtagcgtg gcctgccggg ggctgtccag cgcgcggagg
     1141 gtctcctcgg ccgcccgctc caggaccccc gggggcccga ggcgtcgccg cacctcggcg
     1201 aggtcgccct gcatctgcgc ccgggcctcc ggatcggcga ggagccgctc gacctcggcc
     1261 ctgatgcggg acccgcgcac ctcctcgttg agcagttcag gaacgatccg ccgctgcatc
     1321 acgatgttgg ggatcgagaa ccctacccgg tagacctcgg gggtcaccac aaaccggcgc
     1381 gcgatccagt cggtgagcca ggagagccgg tacacgacca ccatcgggac gccgatgcag
     1441 gccgcttcca ccgaggccgt ccccgaggcc gtcacgatga cctccgagac gcgcatcaca
     1501 tcgagggcgc gcccttcgac cagccgcacc gggaaggaga cgccggccag ggcgcggtga
     1561 atccgcggcc ggaggaacgg cgaggcgacc ggcaagagga actggaccgt ccggcccccc
     1621 cgcaactcgc gggcagcggc aagcatcggc gcgaggaggg cacgaatctc ctggacccgg
     1681 ctgccgggga ggagaccgat caccggctcg cccgacagcc cccactcgcc gcggagccgc
     1741 tcgacggatg cggccgttcc ctctaccgtg tcgaccgccg ggtggccgac gaacacgacg
     1801 tcggcgcctg cgctgcggta ggcgtcggct tcaaacggca ggatcgccag gaggcgcacc
     1861 gcgctctggg ccacccggcc ggccgggtgg ccgcggcgaa agtggatctg cggcgggcag
     1921 aagtacacga gggggagccg tccggaaaag tggcggacca gttcccggtt catcccaggg
     1981 aaatcgacga gcaccagcag atcggggcgg tagcgctcga tcagacggac gagcccccag
     2041 acccggaggg cgaacaccgg aaggcgcacg taggcctcga cgtatccgat gacgccccag
     2101 cggaggctct ctaccagcac gtcgacgccg gcggccgcca tctcggcgcc gccgacgcca
     2161 gcgagcagga ggtcgggacg gcggcgcctg agcgcccgcg ccaagagggc tccctgatga
     2221 tcgcccgaca cctcccccgc cacgatcaag aggcgggggg aggcgccggc cacgcgctac
     2281 gcgcccgcgg gctggacggc cgccgcgacg atcgcgatcc cggaggcggc ggccgcggac
     2341 acgatctgtt cccgctccac cagcagggtc cgtcccgctt cgaccgccag cgccgtcccg
     2401 cgcgaggcgg cgagggccgc gatcgtcgcc gggcccacca cgggaaggtc gtatcgggga
     2461 tcctgctggg ggcggctgac cttgacgacc acggcgccgg cgctgagggc gcccacccgc
     2521 cggatcatct cgtcggtccc ctcggcggcc tcgaccgcga tcaccgcccc gttccgcacc
     2581 gcgaccgcct gcccgatgtc gagcgcggcg atctgagcag cgatccggag cccgacctgg
     2641 atgtccgccg cctccgcact cgacggcgcc cggggggtga gcaccccggg accggggatg
     2701 agatgtccca ggtatcgggc ctggtccccg acctgaaacc cggaggtctc cagcaccgcc
     2761 aggaacgcgc cgacgatgct ggtgtcgcga taatcggacg tacgccgggc caccgactct
     2821 ccgtacgcgt ccaactgggc ccggcggagc gcggggagct tgtccacctt ccccgccagc
     2881 accacctcgc gcaccccgtg gcgccgaaac gcttcgatga tgcccccaag attccccagg
     2941 gtcacggtgt agtaggcgtc ggtgacccgc ggcagttggg gatccccctc gaagatatcg
     3001 acgcagacga cccgccggcc ccggcgccgg gcttccgcca cgacctcgac ggggagcgcg
     3061 ccccgcccgg cgatcaaccc gatcggcgcc tccgggcccg tcaccgcccg atccccatgc
     3121 cccgcgcctg gctctcccgg atgaactcca cgaggtgctg cacctcacgg cagtccccca
     3181 attcctgctc gatccgctcc agggcgtggg acacatcgag ccgggacctc gtgagcagcc
     3241 ggtgggcgcg gcggagcttc agctggacct ccgggggcac ccccgcgcgc tgcaggccga
     3301 cgcggttgat ggtgacaatg cggagcgggt caccttccac gagcatgtac ggggggacgt
     3361 ctttcagaat ccgcgcgagc ccggtcacca tggccagcgt cccgatgcgc gcgaactgga
     3421 tcaccccggc catgccgctg aacaccaccc ggtcctccac gaccacgtgg ccggagagct
     3481 gggtgaagtt ggtgatgatc acactactcc cgatctggca gttgtgcccg gcgtgcgagt
     3541 aggccagcat ctgcgtgtcg ctgccgacca ccgtcgcggc atcctcgccc gtcgcccggc
     3601 tgacgctgac gtactcccgg atcgtcaccc ggtcgccgat ccggaggaag ctgcgctccc
     3661 cctggaagtg ccggtcttgg ggcacgcccc cgagcacggc ggcggggtgg acccggcagt
     3721 cccgcccgag ggtcgtccac tcctcgatca cggcgagggg accgatccac gtccccgccc
     3781 cgatccgcgt gtggctttcg atcaccgcgt gggggccgac gcgcaccccc tcctccagtt
     3841 cggcccccgg gtcgacggtc gccgacggat ggaccagcgg gctcacctga tccggcacgc
     3901 gcgaccggcc ggcccgcgtc atggctcgac ctcctcgagg tccacgatct gtccgtccac
     3961 gacgctctgg ctcaccgcct ccaggtccac catcgagtag gtgatcaccc cgtccgcgac
     4021 gacctcgccg tccacccggc tggtcacccg catcttgccg atccgtcccc gaacacggat
     4081 gacgacgacc tcggtgatca gctggtcccc gggccggacc ggccggcgga accggcaccg
     4141 gtcgagcgcc gcgaagtagg cgagtttgtg ttcggccccc gggagaaggg aggccagcac
     4201 cccgcccacc tgcgccatcg cctccacgat caggaccgcg ggcatgaccg ggtggccggg
     4261 gaagtgtccg acgaagaacg gctcgttcgc agaaacgttc ttgagcccga cgatccgccg
     4321 tccggggtcc agctcgagga tgcggtccac caggatcagc gggtaccggt gggggatgtg
     4381 gcgcatgatc tccttgatat ccatcctcat ccctcccgat cgcgcagcgc cctcgccagc
     4441 gccacgtgca ggccgtggcc cgcgcgcacc gcgatgatat gcccgtggat gtcccggccg
     4501 agcagggcca gatcccccag caggtcgagg atcttgtgcc gcgccggctc gttggggaac
     4561 cggcgagggc cgagataccc ggctcccccg atgaccaggg tgttctccag cgaggcgccc
     4621 agggccaggc cctcgcggcg cagcgtttcc gcctcgcgct cgtaccccca cgttcgcgcc
     4681 ggggcgatga cctcctcgta ccggtccgcc gcggggtcgt acgtcgcggc ctggtcctcc
     4741 ttcgcgggcg cgcccagcgt caccacgcag gcgaccccga accgtggggc cggcagcgcc
     4801 acgacccatg cctggccgtt cccgacccag acgggctccc gcacctcaag gaccggcctc
     4861 acggcatcct ggggccggac ccccgcgccc cgaagtgctt gaaagatggg cagggcgctc
     4921 ccgtccatgc ccgggacctc gctcccgtcg agctccacat ccaggttatc caccccggcc
     4981 gcccaggccg ccgagaggag gtgctccacg gtctgcacgc cgccctcgcc gccgaggctg
     5041 acccgaccgg tggtcccgct gacggcgtcg agggtggccc ggatgggggc gctcccctgg
     5101 gtcgcccgcc tgaagaggat gccggtgttg gggggggccg ggtggagcgt catccgctga
     5161 tgccgcccgg tgtggaggcc gatcccctcg agccggatcg tggcgccgat cgtgccctgc
     5221 cgcgagcggt ccacgatggc gctacgcgcc ttcgagacgc cgcacgcgct gctcgagttc
     5281 ccggagccgc cgcaccaact ccgggagacg ccggtaggcc acttcagccc gccgctgcga
     5341 cctccgcggc ccggccgggt gccccgacac catctcgccg gggggcacat cctgggcgac
     5401 ggcggtgtcc cccatcacaa tcgcgtcatc gccgatctcg atgtgatcgg tgatgccggt
     5461 cagcccggag atcaggactc gattgccgat ccgggtgctg cccgcgatga acaccgccgc
     5521 cgcgatggcc acgtcctcac cgatctgcac gttgtgggcg atgtggatca aattgtcgag
     5581 cttggttccc cggccgatcc gcgttgcccc cagcgtcccc cgatcgaccg tgacgttggc
     5641 cccgatctcc acatcgtcgt cgatcacgac gcggcccacc tggggaatga gcacgtggcg
     5701 gcgctgcgcg tcttgcgcgt acccgaaccc gtcgctcccg atcacggtcc cgctctggat
     5761 cacgacgcgc gcgcccaggc tgcacccgtg gcggatggtc acgcgcgcat ggagcacgca
     5821 ctccgggccg atctggaccc cgtcctcgac gacgccgtgc gcgtgaatct gtgcccgcgc
     5881 tccgatcgtc acgtcgtcgc cgacggtgac cagcggcccc aggaacgcgc cgtcgccgat
     5941 gcgggcccgc gacccgatga cgcaggtcgg gtggatgccc gaggggggcg tcttcggtgg
     6001 gacgaacgcg cgaatcgcca gggcgagcgc caggcggatg ttgccgacgc ggatcgtcgg
     6061 cagatgggtc tccggggcat catgggacag gatcaccggg gcgcgggccg cctcgacctc
     6121 ggggaggcgg cggggatcgg ccaggaccac cacggtgccc ggccgggcct cctccggggg
     6181 caccacctgc tggacctcga tgctgggggc cccgacgagg tccccgccga cgcgctgggc
     6241 cagcgcatcc aaccgcatcc tgggctccgt cgcttacttg agccggtcga tgacctgctg
     6301 ggtgatgtcc cggccgccga accacagggg agccttgacc aggaccagct tcagatgatc
     6361 ttccctggcg atctccccgg cgacctggcc cagctcctgg gtcagctgcc cttcgaggtc
     6421 gcgcttcatc tgtcccagct cggtgagata gcgggcccgc cgaccgctca ggtcggccgg
     6481 ggtgatctgg gtcgcgaccg cggccagttc cgccaccatc tgcttctcgc gattatccag
     6541 cagcttctgg tactgcagcg ctttgacgct ctcgttgagg acgcgctgtg tgtcgacgat
     6601 cccgacaggc gccgcgccgc accgcccgac ggagaacgcg gcgcccagcg ccagcagccc
     6661 aaccaggatc cacgtcatgc ccgcccccct ttaccgcctg atgcgggcga tgagttcgtc
     6721 ggtggcatcg accgttcccg gcgcgacgat cgcctgggtg agcaccacat cccacccctt
     6781 ctcttgcgcc agtgccgccg cctccacccg cagatctgcc aggatcaccg caaagacccg
     6841 cgcccgcccc agctggacgc cgcggagctc ttcctggagc gcgcgaatcc gtgccgtctc
     6901 ggcctccacc agggccctgt accgcgcttc cgcctcgcga gcctgcgtcc cgcgggcggc
     6961 ggacacgatc gcctgctcct gtccccgcag ccgctggttg aactccgcct gtttggcggt
     7021 gagctgggcg cgcacctgct ccgtcacctt tgccgcgcgc tcgttgacca gctgctgccc
     7081 ctccttggcg agctgcgcct ggtaggcctg caactcggcg ctggaggcct ggacccgctc
     7141 cttctgaaag tcctcgaggg cctggcggtt ggccttttcg tgcgccgcga tcttctcatc
     7201 gcgctcgttg gtaagcgcct ggatctgttg ggtgaggcgg tcgctttccc ccttgccggc
     7261 cggctggagg gtttccagtt tgagcttgag gttcaggagc ggcagccggt actcacccat
     7321 cgtctgctgc tggaactgct gggtgtccgc ctccagcgcc tgctgtttgt cgcggacggc
     7381 cttggtcagc tccgcctcca gttccgcgcg cttggcctcc gccttcttct ggtcctcgac
     7441 ccggagctgg gcgacgtagg cgtccatatc ctggcgggcg gcagccttca ccgcctccgc
     7501 ctcgcgctgg aactcgggct gcatccgctc cacggcggcc tgcatctccg gggtcaggtc
     7561 gatcttcggc ggttcgatgc ggcctgcggc gcccgacgtc tcaagggcca gtttggcctg
     7621 cagttcgctg acctgccggt cgagcaccgt cacctccggc caccggggat gcacccgggc
     7681 ggcgcgcgcg aggtcgacga cggcgatccg gagggcgcgc ggccccgcct ggggcgacgc
     7741 ctcggatgcc ggggagctcg acagcggatg cgccgccggc gtccgcccac cacacccgac
     7801 gacgagcgcc gcgagcgccg cacacacgaa cgcgcgactc atccgaccgg ggtcacttgg
     7861 cgctgatcgc cttgacgacc tggtcggtca ggtcgacccc gccgtagagg acgacctcgc
     7921 ggctcagcac gatgctgatg ccctggtccc gggcgatctt ttcgatcgcg gcgcgaatgt
     7981 ccttctcgag gcccccgatc agctcctggc gcttcttcag caactgcgcg ttgacctcgc
     8041 gctggacctc ctcgcgctgg ccggctgcca tccccttcat ccgctgctgc gcgtcggcga
     8101 tctgcgtctg ggcgtagtcc ttgagcgccc cttccgcgct cgccttgcgg ggatgggcct
     8161 cgatcgccct gggcatgtcc acatagccga tcgtcaccga ctgcccgagc gccggggcgg
     8221 atcgggcgac gacgaatccg acggcggcca cgacgacgat cgccgccgcg acgatgctga
     8281 tggtcttcgc gttccacctc atccacgttc cctccctacg cgattttccg cggttcaaaa
     8341 ctgcgcaccg gccgagaccc agaactgctg gccaaagccg ctgatgccgt aatcgatccg
     8401 gaacggcccg accggtgtct tgacttgaat cccgatcccc acatcgggct ggaggttaaa
     8461 cgtgctggtc ccggcgggct ctgcatcccc cgcatcgatg aagaggaccg tcgtgatccc
     8521 cgtaaacgac tgcatgaacg ggagccagct gaggggaaag cgatactcca cctgccccag
     8581 caccacgtcg tcgcctcgga gaaagccggt gggatacccc cgcagcgtgg attggccgcc
     8641 gagataatac tgctcctgga ggggcagggg aacggccgag tatcccaggt gggcgtgccc
     8701 cacgatcgtg ctatcccccc cgagcggaaa gaaatgcgcg taatcgaaat cgtacttctg
     8761 aaactggaac gttcccccca gggccagaag cgcgaactcg ccgcccaggg tgatgtgctc
     8821 gccgctgatc ggggtttgtg gatcgttccg cgtgtcctgg ctgacgccca ggatcagcgc
     8881 gttgacctgc ccgggcgttg caaacgcggc gaaattaaac ccgggggg
//
LOCUS       DASQGH010000033         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11449, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000033 DASQGH010000000
VERSION     DASQGH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11449"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..509)
                     /locus_tag="VEW91_00580"
     CDS             complement(30..509)
                     /locus_tag="VEW91_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYM90111.1"
                     /translation="MGLYRIWPLRPGTRRVWNPWQELEELRRAMEEITARADVPALSS
                     EMGAFAPGVDLYDTGKAFVAWVDLPGVRAEDLILTIEDGVLTIGGHRPAVRPEGATEL
                     CCERPAGRFARTLQLPAEVDAKGVKATLRAGVLEIGLPKSGGGSPRKVEIEVARRED"
     gene            747..1349
                     /locus_tag="VEW91_00585"
     CDS             747..1349
                     /locus_tag="VEW91_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYM90112.1"
                     /translation="MVKDRMTSPAITTTPETETRQALQTMYVHKIRRLPVVTERGDLL
                     GIVTQRDLYEKGEAVTPVSAIMTAPPYTTGPDVPLVQAALLMRNLGVGALPVVERGRV
                     VGIITESDIFDAFLELLGANRAGTRLIVPLPDISGGVARLLQALQPTGVPLTGLTTYV
                     DGGRPSAIVTADERDPRDLVRAIRAAGFEPTLISVQGTAA"
     gene            complement(1371..2063)
                     /gene="hypB"
                     /locus_tag="VEW91_00590"
     CDS             complement(1371..2063)
                     /gene="hypB"
                     /locus_tag="VEW91_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HYM90113.1"
                     /translation="MPPRIVEVRQNILKANDLRARRLREAFAHAGVYVVSLVSSPGSG
                     KTTLLCATLRQLRGRYRVAALVGDLATDHDAARLRESGAPVCQITTGTVCHLDAEMVE
                     RAVATWDRTPLDFLFIENVGNLVCPAGYDLGEDLRAVLLSVTEGEDKPQKYPAIFHSA
                     DLAIITKVDLAAAAEYDEALARRCLEEVRPDLEILRVSAKSGAGVDGWLRRLEAGLLR
                     KRGGVPDPMRTV"
     gene            complement(2044..2412)
                     /locus_tag="VEW91_00595"
     CDS             complement(2044..2412)
                     /locus_tag="VEW91_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HYM90114.1"
                     /translation="MHELSIALSLVDVATSHLRGTCAGRRVDAVHLRLGPLAGVDPDA
                     LRCSFTLACRGTVLDGARLVIEEVPVVAYCAGCGRERVVPGIVRLRCPACGSLMPVVR
                     RGDELELTALEVEDDAAPDR"
     gene            complement(2388..>2606)
                     /locus_tag="VEW91_00600"
     CDS             complement(2388..>2606)
                     /locus_tag="VEW91_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HYM90115.1"
                     /translation="GRVMDMVDAEARTARVDVLGTPRVINLGLLEGVGVGDWVLVYMG
                     CALERIGEAEAAEAVRLWEELCTSFPSR"
BASE COUNT          410 a          996 c          805 g          395 t
ORIGIN      
        1 tgcccacttc acctcggcac ctcacccggc tagtcttccc gtcgtgcgac ctcgatttcg
       61 acctttcgag gggatccccc cccgctcttc gggaggccga tttccagcac gccggcgcgc
      121 aacgtggctt tcacgccctt ggcgtccacc tccgccggca gctgcagcgt gcgcgcgaag
      181 cgtccggccg ggcgctcgca gcacaattcc gtcgccccct cgggtcgcac ggccggacgg
      241 tgcccaccga tcgtgaggac cccgtcctcg attgtgagga tcaggtcctc cgctctgacg
      301 cccggcagat ccacccacgc cacgaaggct ttgcccgtgt cgtacagatc gacgccgggt
      361 gcaaaggccc ccatctcact cgaaagcgcc ggcacgtccg cgcgtgcggt gatctcctcc
      421 atcgcccgcc ggagctcctc gagctcctgc cacgggttcc acacgcgccg cgtccctggc
      481 cgcaacggcc agatgcggta caatcccatg aggggcctcc ttccctcagc cccagcctgg
      541 ctcgtaccca ccccaatgtt cttcatagca gcgcgccctg gggggcacca tcggattggc
      601 gcccgatttc cgctacggca ctggggggat tctgggagtc gcctacgaac cttggggaga
      661 ccgaatccgg tccccacccg ataggctctc tccgggtggg aaggtatagt aactttgagg
      721 cgcttcgtgg ggaggatgcc atgcagatgg tgaaggaccg aatgaccagt ccggcgatca
      781 cgacgacgcc cgagactgag acacgccaag cgctccagac gatgtacgtc cacaagatcc
      841 gccggctccc ggtcgtcacc gagcggggcg accttctggg catcgtcacc cagcgcgatc
      901 tctacgagaa gggcgaagcg gtcaccccag tgagcgcgat catgacggcc cccccgtaca
      961 ccacggggcc ggacgtcccg ctcgtccagg ccgcgctcct catgcgcaac ctgggcgtcg
     1021 gagcgctccc ggtggtggag cgcggccgtg tcgtgggcat catcaccgag agcgatatct
     1081 tcgacgcctt cctcgagctg ctcggggcca atcgggcggg aacccggctc atcgttcccc
     1141 tccccgacat ctcaggaggc gtcgcgcggc tcctccaggc gctccagccc accggcgtgc
     1201 cgctgaccgg cctcaccacc tacgtcgacg gcggccgccc ttcggcgatc gtgacggccg
     1261 acgagcgcga tccgcgcgat ctggtccgcg cgatcagggc tgccggcttc gagcccacgt
     1321 tgatcagcgt ccagggtacg gcggcctagc cggctggacg catcgcccgc tcagaccgtg
     1381 cgcatggggt cggggacgcc tccgcgcttc cggaggagcc cggcttccaa ccggcgcagc
     1441 cacccgtcca cgcccgcccc ggacttggcg gagacccgca ggatctcgag gtcgggccgg
     1501 acctcttcca ggcagcggcg ggccaaggct tcgtcgtact ccgccgcggc ggccaggtcc
     1561 accttggtga tgatcgccag atcagccgag tggaagatcg cggggtactt ctggggcttg
     1621 tcctctccct cggtcacgga caggagcacc gcgcgcagat cctctcccag atcgtagccc
     1681 gccgggcaga cgaggttccc gacgttctcg atgaacagga aatcgagggg ggtgcggtcc
     1741 caggtcgcca cggcgcgctc caccatctcc gcgtccagat ggcacaccgt gccggtggtg
     1801 atctgacaga ccggagcgcc gctttcgcgc agccgtgcgg cgtcgtgatc ggtggccaga
     1861 tcacccacga gcgccgccac ccggtaccgt ccccgcagtt gccgcagtgt agcacacagc
     1921 agggtggtct tccccgaccc cgggctcgag accaggctga ccacgtacac gccggcgtgc
     1981 gcaaaggcct cgcgcaaccg acgggcccgc aggtcgtttg ccttgaggat attctgccgg
     2041 acttcaacga tccggggcgg catcgtcctc cacctccaac gccgtcagtt ccaactcgtc
     2101 accgcgtcgc accacgggca tgaggcttcc acaggcgggg cagcggagtc tcacgatgcc
     2161 gggcacgacg cgctcgcgcc cgcaccccgc acaataggcc accaccggga cctcttcgat
     2221 caccaacctg gccccgtcca gcaccgtgcc ccggcaggcg agggtgaacg agcacctgag
     2281 cgcgtccggg tccactccgg caagcggccc gagccggagg tgcaccgcgt ccacgcgccg
     2341 tcccgcacac gtcccgcgga ggtggctggt cgccacgtcc accaggctca gcgcgatgga
     2401 aagctcgtgc acagttcctc ccagagccgc acggcctccg cggcctcggc ctccccgatt
     2461 ctctccaacg cgcaccccat gtagaccagc acccagtcgc ccacccccac accctcgagc
     2521 aggcccaggt tgatgacgcg gggggtcccc aaaacgtcca cccgggccgt gcgggcctcc
     2581 gcatcgacca tgtccatgac ccgtcc
//
LOCUS       DASQGH010000034         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5766, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000034 DASQGH010000000
VERSION     DASQGH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5766"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VEW91_00605"
     CDS             complement(<1..219)
                     /locus_tag="VEW91_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90116.1"
                     /translation="MAVAAERAQSRGAGRLPGGALVGAVVLAALLVVALLGERAAPYD
                     PEQVSGAVYQPPSRVHLLGTNDLGQDILS"
     gene            complement(219..1199)
                     /locus_tag="VEW91_00610"
     CDS             complement(219..1199)
                     /locus_tag="VEW91_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90117.1"
                     /translation="MRGAFLRFLLLVAAVVVLTFALPRAFPGDPTEALGDTGGASVRV
                     LPEEARAALRAYYGLDRPLPVQFGRYLVRLEHLDLGQSFALHRPVRALIGERLPYTLA
                     LVGTSLAVSAALGIALGILSAWRAGSTLDRWLLGGFLGIGALPEFLVGLVLIVAFAVR
                     WPVLPASGATTPFLSADTPGGLAQAWDRLRHFVLPGLTLTLGSLPHFFYLMRNATVPV
                     RDAPYLVTARAKGLTEGRLAARHAARSAVLPVVSLFGIRVAFVVGGVLVVERLFGYPG
                     VGLLMVQAVWARDYPVLEGCFLLLSLSVLAWNLLVDGILMRVDPRIREAE"
     gene            complement(1212..2813)
                     /locus_tag="VEW91_00615"
     CDS             complement(1212..2813)
                     /locus_tag="VEW91_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90118.1"
                     /translation="MPALRRSRVPLAIVLCVIAARAFTAAGSASPSADVLRLVWWTDV
                     GFPTPFAFSTVGPGGVVRLQLIYDTLTWKDARGLIPWLATSWQVSPDGRTYTFTLHAD
                     VRWQDGRPLSADDVRFTFEYYKRHPFRWVSVARVSQVAVRDPRTVSISLAEPYAPFLS
                     EIAGIVPILPAHIWRDIEDPAQAQDLRATTGSGPFRLAGYSAVTGQYLFRAYPGYFRG
                     APGFRELRYQVLPTEQQQLLAVQQGQVDAAVTTDYAAAESLRTHPPLRVLETEPLSVV
                     RLVFNTRRPPFDDRRFRQAIASALARRQLASLVTHGPALLGGPGIIPPGTAWSNPAVK
                     QVPYDPAQAAVLLDRLGTRMGADGLRRTAAGTPVKVDLLTDPSAPDAPLIQQMLRRVG
                     IEVALLPADPKTRSALVREGKFQMLLTTHIGVGGDPDFLRRWFTGEQANAFALGDALD
                     NEEFHRLASQQVRTLDAGARRQMVFRMQEILADEMPTLPLYYRRFYWVYNGAKLHPFE
                     TLGGLLDGIPLIENKLAFLRPPSQP"
     gene            complement(2817..>3108)
                     /locus_tag="VEW91_00620"
     CDS             complement(2817..>3108)
                     /locus_tag="VEW91_00620"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HYM90119.1"
                     /translation="ISWGTVTAVLGDDLAVDRRPLVLRGDDLVLGDTEPRTVRWRFNG
                     KALVDAQPGDVVSIHWGCACDRLTPVQLAHLRHETSFHLTLANRRHRPGVPV"
BASE COUNT          540 a         1056 c         1121 g          391 t
ORIGIN      
        1 gctgaggata tcctgcccga ggtcgttggt gccgagcagg tgcacgcgcg aagggggctg
       61 gtacaccgcc cccgacacct gctcgggatc gtacggtgcg gcgcgctctc cgaggagcgc
      121 gaccacgagc agcgcggcca gcacgacggc gcccacgagc gctcctccgg gtagacgacc
      181 ggccccccgg gactgcgcgc gctcggccgc aacggccatt actccgcctc ccggatccgc
      241 ggatccacgc gcatgaggat cccgtccaca aggagattcc acgccagcac ggagaggctg
      301 aggagcagaa aacacccctc gagcacggga tagtcccgcg cccagacggc ctgcaccatc
      361 aagagcccga cccccggata cccgaagagg cgctcgacga cgagcacgcc gccgaccaca
      421 aacgccactc gaatcccgaa gagactgacc accgggagca cggcgctgcg ggcggcatgg
      481 cgggcggcca ggcgcccttc ggtcaacccc ttcgcccgcg ccgtgacgag gtagggggca
      541 tcgcgcaccg gcacggtcgc atttcgcatc aagtagaaga aatgggggag gctgccgagc
      601 gtcaacgtga gtccgggcag gacgaagtgc ctgaggcggt cccacgcctg cgcgagcccg
      661 cccggggtgt cggcggacaa aaacggcgtg gtcgcaccgc tggccgggag caccggccac
      721 cgcaccgcga aggcgacgat caggacgagc ccgacgagaa actcgggaag cgcgccgatc
      781 cccagaaatc ctccgaggag ccaccgatcc agcgtgctgc ccgcccgcca cgccgagagg
      841 atgcccaggg cgatccccaa cgctgccgag acggcgaggc tcgtgcccac cagcgccagc
      901 gtatacggca gtcgctcacc aatgagcgcg cgcacgggcc ggtggagcgc aaaggactga
      961 cccaagtcga gatgctcgag gcggaccagg taccgcccga actggaccgg gagcggacgg
     1021 tcgagcccgt agtaggcgcg gagcgccgcc cgggcttcct cggggaggac gcggacgctg
     1081 gcccccccgg tgtcccccag cgcctcggtc ggatcccctg ggaacgcgcg gggcagggca
     1141 aacgtgagca ccaccaccgc ggcgacaagg aggagaaagc ggagaaaggc gccccgcatg
     1201 gagtcgggcg gtcagggttg gctgggcgga cggagaaagg cgagcttgtt ctcgatcaac
     1261 gggatcccgt ccaacaggcc gcccaaggtt tcgaacgggt ggagtttcgc gccgttgtag
     1321 acccagtaga accgccggta gtagaggggg agggtcggca tctcgtcggc gaggatctcc
     1381 tgcatacgga aaaccatctg ccggcgggct cccgcgtcca acgtccgcac ttgttgggat
     1441 gccaggcggt ggaactcctc gttgtcgagc gcgtccccca atgcaaacgc attggcctgc
     1501 tcgccggtga accaccgccg taggaagtcc gggtcgcctc cgaccccgat gtgcgtggtc
     1561 aggagcatct ggaacttccc ctcacggacg agcgcgctcc gggtcttggg gtccgccggg
     1621 aggagcgcca cctcgatgcc aacgcgccgc agcatctgtt ggatgagcgg ggcgtccgga
     1681 gcgctgggat cggtcagcag gtccaccttt accggcgtgc ccgcggccgt ccgccggagc
     1741 ccatccgctc ccatgcgggt gcccaaccgg tcaagcagga ccgcggcctg tgcgggatcg
     1801 taggggactt gcttcacggc cggattgctc caggcggtgc cgggggggat gatgccgggc
     1861 ccgcccagca gcgcgggacc gtgggtgacg agcgaggcga gctgcctccg cgccagcgcg
     1921 gaggcgatgg cctgccggaa ccggcgatcg tcgaacggcg ggcgcctggt gttgaacacc
     1981 aggcggacca cggagagcgg ctcggtctcc agcacgcgca gcggggggtg ggttcgcagg
     2041 gattcggctg cggcgtagtc ggtcgtcacc gctgcgtcga cctggccctg ctggaccgcg
     2101 agcagttgct gctgctccgt cgggagcacc tgataccgaa gctcgcggaa gcccggggct
     2161 cccctgaagt agccggggta cgcccggaag aggtactggc cggtgaccgc gctgtacccg
     2221 gccagccgga aggggccgct gccggtcgtc gcgcgcaggt cctgcgcctg cgcgggatct
     2281 tcgatatccc gccagatgtg cgcggggagg atcgggacga tgccggcgat ctcggagagg
     2341 aagggcgcgt aaggttcggc gagggagatc gagaccgtcc gcggatcccg caccgccacc
     2401 tggctgaccc gggcgacgct cacccaccgg aatggatgcc gcttgtagta ctcaaaggtg
     2461 aagcggacat cgtcggcgga gagcgggcgc ccgtcctgcc accggacatc ggcgtgcagc
     2521 gtgaacgtgt aggtgcggcc gtcgggcgac acctgccagg acgttgcgag ccaggggatg
     2581 agcccccggg catccttcca ggtcagggtg tcatagatga gctgtaggcg gacgaccccg
     2641 ccgggcccga ccgtcgaaaa cgcaaagggc gtggggaacc ccacatcggt ccaccacacg
     2701 agcctgagca cgtcggctga gggggaggcc gaccctgccg cggtgaatgc gcgggcggcg
     2761 atcacgcata ggacgatcgc cagggggacg cgcgaccgcc ggagcgccgg catgcctcag
     2821 accgggaccc cgggcctgtg cctccggttg gccagcgtga ggtggaaact ggtctcgtgc
     2881 ctgaggtgcg caagttggac cggcgtgagc cggtcgcacg cgcatcccca gtggatggac
     2941 accacgtccc ccggctgcgc gtcgaccaag gctttgccgt tgaaccgcca ccggaccgtc
     3001 ctcggctcgg tgtcgccgag gacaaggtca tccccccgga ggacgagcgg cctccggtcc
     3061 accgccagat cgtccccgag cacagccgtg accgtgcccc aactgatc
//
LOCUS       DASQGH010000035         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34082, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000035 DASQGH010000000
VERSION     DASQGH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34082"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VEW91_00625"
     CDS             <1..418
                     /locus_tag="VEW91_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90120.1"
                     /translation="VLGFRLRALGLRRVFRRRAAIYHYRPDISFETLPALFAKEEERA
                     ATALRFLTKNPTLEARFAICHTPVHRMLNAVQRGFGAFHPGNAVVWIARLRRWGLGGP
                     ARIILAGVLRGHYFACLLAFEAAARSNGTQRHYPYV"
     gene            387..1457
                     /locus_tag="VEW91_00630"
     CDS             387..1457
                     /locus_tag="VEW91_00630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYM90121.1"
                     /translation="MELSVIIPTYNNGGVLARTLDALLAQRCSPDLYEIVIVDDGSTD
                     GTAEMVRRLHSPVPIRYVAQPNRGRAAARNLGARVARGRILLFLDSDFFAAPDLLAAH
                     VKHYPPAARRIGIQGASRTHPDTLITPFMQGREVRLEVSPGPPGRISLFRISTRNLSL
                     LRAEFIDVGGFDEAFGGYGWEDIELAWRLYARGVRFTYEPRALGDHYQVQDLEGLREK
                     MRQGGKAAVYFWWKHGRSWWLGLRLEVAPVLLPIKWLVYRTRLLTPLVRWLLPRAEAR
                     GWRSLVSECTNHLVWEAYYEGVFSVLAEGRSGRHAFRRHLGEGVEGARLATPHGGGLQ
                     EDQGPGPLGDRVEARRSPPEVR"
     gene            complement(2271..2975)
                     /locus_tag="VEW91_00635"
                     /pseudo
     CDS             complement(2271..2975)
                     /locus_tag="VEW91_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
     gene            complement(2976..>3429)
                     /locus_tag="VEW91_00640"
     CDS             complement(2976..>3429)
                     /locus_tag="VEW91_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90122.1"
                     /translation="HLSTDRLRRLVATGGGVWVDARRFRFDGIDVTVTTDHYADALRC
                     ATVVLGMAGGANEQAAGLGKPVVAFPGTGPQFTPRFLEEQQRLLGDALVATSTWRDAA
                     QALATLLGDPRERERRGQVGLERLGGTGSAQAIAQRLLDRLALNRLSA"
BASE COUNT          551 a         1222 c         1215 g          441 t
ORIGIN      
        1 cgttctgggg ttccggctcc gggcgctggg cctgcgccgg gtgttccgcc gtcgggccgc
       61 catctatcac tatcgccccg acatctcgtt cgagaccctg cccgcgctct ttgcgaaaga
      121 ggaggaacgc gccgcgaccg cgctccggtt cctcacgaag aaccccacgc tcgaggcgcg
      181 gttcgcgatt tgccacaccc ccgtccaccg catgctgaac gccgtccaac gggggttcgg
      241 cgccttccac ccggggaacg cggtggtctg gatcgcgcgg ctcaggcggt gggggctggg
      301 ggggccggcg cgcatcatcc tggccggcgt gctcagggga cactacttcg cctgcctcct
      361 cgcgttcgaa gcggcggctc ggagcaatgg aactcagcgt cattatccct acgtataaca
      421 acggcggcgt cctcgcgcga acgctcgacg ctctcctggc ccagcggtgc tcccccgacc
      481 tgtacgagat cgtcatcgtg gacgacggct cgaccgatgg gaccgccgag atggtccggc
      541 ggctccacag ccccgtcccg atccggtacg tcgcgcagcc gaaccgtggc cgggcggccg
      601 cgcgcaacct gggcgcccgg gtcgcccggg ggcgcatctt gctgttcttg gactccgatt
      661 tcttcgccgc ccccgacctc ctggccgcgc acgtcaagca ttatccccca gccgcgcggc
      721 ggatcggcat ccagggcgcc agccggacgc accccgacac gctgatcacg ccgttcatgc
      781 agggccggga ggtgcgcctc gaggtctcgc cggggccgcc cggccggatc tcgctgttcc
      841 gcatcagcac ccggaatctc tcgctgctgc gagcggagtt catcgacgtg ggcggattcg
      901 atgaggcgtt tggcggatac gggtgggaag atatcgaact cgcatggcgc ctgtacgccc
      961 gcggagtgcg gtttacctac gagccgcgtg ccctcggcga ccattaccag gtgcaggacc
     1021 tggaaggact ccgtgagaaa atgcggcagg gcggcaaggc cgcggtgtac ttttggtgga
     1081 agcacggccg gtcctggtgg ctcggcctcc gcctcgaagt cgctcccgtg ctgctcccga
     1141 tcaagtggct ggtctaccgc accaggctcc tgaccccgct ggtccgctgg ctgctccccc
     1201 gggccgaagc gcgcgggtgg cggtccctcg tgagcgagtg caccaatcat ctggtgtggg
     1261 aggcctacta cgagggcgtc ttcagcgtcc tcgccgaggg gcgctcaggg cggcacgcgt
     1321 tccgccgaca cctcggggag ggcgtcgagg gcgcgcgcct ggccacaccg cacggcgggg
     1381 gccttcagga ggaccagggt cccggcccgc tcggcgaccg ggtcgaggcg cggaggtctc
     1441 caccggaggt ccgctagttc cccgcgggtg atgacgagga agacccgccc gggggcgcag
     1501 aggcgagcgc gcagcgcgtc ttcggtgtcg gcccactcga tgtgccggcg ggtgtagaag
     1561 accagcgagg tcgcggtgct catccggtag gccacgacgc ggtcggcggg cccgagggcc
     1621 gcggtgatcg cctgggcgag gggccggatc ggcttctgcg cctcggcgag cggcagcacc
     1681 cacgtcagca acgcgaacca ggtggcggcc atcaccgcgc acagcgcgcc gaacacggcg
     1741 atcggcggcc cccgcagcga gaccaggagc acgaccccga tcccggcgag gagcacgcct
     1801 cccgggatga gcaacgccgc ccccgaggcc cggaacgcat caggatactg cgcgcccagg
     1861 accgccacaa ccgtccagaa cagcgctccg atgagcgcca ggagcagcgc gagcgaggcc
     1921 ccgatcggcg cgtccacccg gcggcgctcg agcaccccct cccacagcac ggccaccccg
     1981 atcgccgcaa aggggaagat cggcacgatg tacccgggga gtttggtctg cgccgcggtg
     2041 tagaacacca ggggcacacc cgtccccagg aggacgagca ggctgccgtc cgcctctcgc
     2101 caccgccgga ggtgcagcgc ggccgccgcg ggccaaaacg tcgtccaggg aaacgccccg
     2161 aggagcgcca ccggggcgta gaagtaccag ggagcggagt gcttctccac caccccaaag
     2221 aacctcccga gtgtatactg gccgacgttc gaggcgatga aggtccaccc gtggaggagc
     2281 gtctccgcca cgtaccacga gagtcccaca gccgcgtaca cgatgagccc ggggatccac
     2341 gggacctcgc gccagcgacc ccaggcgcgg cgcacggtga cgaacgccgc gatcacgagc
     2401 cccgggagga ggagcccgat cggccccttg gtgaccgtcc cgagccccgc gaagagaaag
     2461 aaccggaggt acgcgcccgg ccggcccgag cgatagccct ggaggaatgc gtagaacgcc
     2521 agcagcatcc aggccatcag gaccgtgtca aagaccgcca gccgggactg catcaggtac
     2581 tggagggtca gggccaggat cgcgccggcc agcagcccca cccgggcgcc gaagagggcg
     2641 cggccgagca gcacggtcgc gtacacggcg aggacgctga agaccacgga ccagatgcgg
     2701 atcgtgaact cagagaatcc caggagacga ccggtggccg ccacgagcca catgtagaac
     2761 ggggggtgca cgaaccaggg ctgaccgttg acgtgcatcg tcacgggatc gccggtttgg
     2821 acgatctcca ccgcgatctc gccgtactgg ctctcgtcct ggtcgaagag cgccggggca
     2881 cccagccggt acagatcgag cgcggcgcag gcggcgagca ggagaacgat ccagtagcgt
     2941 tccctggggg cccgcggacc ggggggggca gccacctacg cgctgagccg gttgagggcc
     3001 agccggtcga gcagccgttg ggcgatcgct tgagccgacc ccgtgccccc gaggcgctcg
     3061 agcccgacct ggccccggcg ctcccgctcg cgcgggtcgc ccaggagggt ggcgagggcc
     3121 tgggccgcat cccgccaggt ggaggtcgca acgagcgcgt ctcccaggag gcgctgctgc
     3181 tcttccagga agcggggcgt gaactggggg ccggtcccgg gaaacgcgac cacgggcttg
     3241 cccagcccgg ccgcctgctc gttcgcgccg ccggccatgc ccaggacgac ggtggcgcac
     3301 cgcagcgcat cggcgtagtg gtcggtggtg acggtgacat cgatcccgtc gaaccggaag
     3361 cgccgcgcgt ccacccacac ccctcccccg gtcgccacca ggcggcgcag ccggtcggtc
     3421 gagagatgg
//
LOCUS       DASQGH010000036         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36942, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000036 DASQGH010000000
VERSION     DASQGH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36942"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VEW91_00645"
     CDS             <1..586
                     /locus_tag="VEW91_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90123.1"
                     /translation="NIIVSSSTAPGGSGNCSYTDFKIHASDTDPNATTVVNINSLQMG
                     TCTRLTVLGVGKVDLRIGAPTGVGLYVGGDIPTAAHFGVDQNDKWNAPSPLPGGQLTV
                     WINSSNMTCSNSGGIPTPSCAGMISHATGSATIFTPNGSFNYDDSLGSLGYSFNGPLV
                     ANKILLTGYNKINVGAGTNFMYGTFNKLRSWKDQ"
     gene            complement(589..>2290)
                     /locus_tag="VEW91_00650"
     CDS             complement(589..>2290)
                     /locus_tag="VEW91_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYM90124.1"
                     /translation="GGVCRGAICVIDLDMVSQTRLVAYNKAIAQWTRLSGSAEERNAA
                     EYAEEQLRGFGLATRILTHDAYISLPRSASLRLMRPQQQEIFCITHSMGIPTPPEGVA
                     AELVYAGRGTPDDYAAARAAGKVALVEGRATPQHAVNATRAGALGLICISGRHAHEMC
                     CSPVWGNPSAETVAALPRVHLLSVHRADGEALRGLCRRGRVEVQFTAAVETGWTRTPI
                     VLAEVSAGHPEAEAGMFVLFSGHLDSWYLGAMDNGSANAATLEATRILALHRERLRRG
                     LRVALWSGHSHGRYSSSAWYADNHWFDLADHCLAHVNIDSVGGIDADEFVTNSMPETA
                     GLGTWAVQQVTGATLIPKRVGRNSDQSFLGIGIPSILGSVSHQADGTLGWWWHTPHDT
                     LDKVDPVRLLRDAKIFVRVLERLLTDPVLPLDYEASARDVRESLEALTKDVGPALDLG
                     PAISAASALEERCARLARVAATATPAQVRIINACLVALGRALIPATYTSAGNYAHDPA
                     LETEFLPALRPARRLASLALESDAAKLLSIDLIRGRNRVTGALRQACRDVDACLAQVV
                     "
BASE COUNT          377 a          782 c          772 g          359 t
ORIGIN      
        1 caatatcatc gtatcgagca gcaccgcacc cggcggatcg ggcaactgct cgtacacgga
       61 tttcaagatc cacgccagcg acaccgaccc caacgccacg accgtggtga acatcaactc
      121 gctccagatg ggcacgtgta cccgtctgac cgtgttgggc gtggggaagg tcgatctgcg
      181 catcggcgcc ccaaccggcg tcgggctgta tgtggggggg gacattccta cggccgccca
      241 tttcggcgtg gaccaaaacg acaaatggaa tgcgccctcg ccgctccccg gcggtcaact
      301 gaccgtgtgg ataaattcgt cgaacatgac gtgctccaac agcggcggca tacctacccc
      361 gtcgtgcgcg ggcatgatct cccacgcgac ggggtcggcc acgatcttca cgccgaacgg
      421 ttcgttcaac tatgatgact ccctggggtc gttgggttat tccttcaatg ggcccctggt
      481 ggccaacaaa atccttctga ctggttacaa caaaatcaac gttggggcag gcaccaattt
      541 catgtacggg accttcaaca aactgcggtc ctggaaagac cagtaggttc acaccacctg
      601 ggcgaggcag gcgtcgacgt cccggcaggc ctgccggagg gcgccggtga cacgattgcg
      661 accccggatg agatcgatcg acagcagttt cgccgcatcg ctctcgaggg cgaggctcgc
      721 cagccgccgg gccggccgca gcgccggcag gaactcggtc tccaacgccg ggtcgtgggc
      781 gtagttgccg gctgaggtat aggtcgcggg gatcagggct cggcctaggg ccaccaggca
      841 ggcgttgatg atcctgacct gggcgggcgt cgcggtggcc gccacccggg cgagccgggc
      901 acaccgttcc tccagggcgg aggcggcgga gatcgccggt cccagatcca gcgccggtcc
      961 cacgtccttg gtcagcgctt ccaggctctc ccgtacgtcc ctggcgctcg cttcgtaatc
     1021 gagggggagc accgggtcgg tcaggaggcg ctccaggacc cgcacgaaga tcttggcgtc
     1081 ccgcaggagg cgcaccggat cgaccttgtc cagcgtgtcg tgcggcgtgt gccaccacca
     1141 gcccagggtg ccgtcggcct ggtgggagac cgagccgagg atcgacggaa ttccgatgcc
     1201 gagaaaggac tggtcggagt tccgccccac gcgcttcggg atcagggtgg ctcccgtgac
     1261 ctgctggacg gcccacgtgc ccagccctgc cgtctcaggc atggaattgg tcacgaactc
     1321 gtcggcgtcg atgccgccga ccgagtcgat gttgacgtgc gcgaggcagt ggtcggcaag
     1381 atcgaaccag tggttgtcgg cgtaccaggc ggacgacgag taccggccgt gggagtgccc
     1441 cgaccacagg gccacgcgca atccccgccg cagcctctcg cggtggaggg ccagaatgcg
     1501 ggtcgcttcc agcgtcgcgg cgttcgcgct gccgttgtcc atcgcgccca gataccagct
     1561 gtcgaggtgg ccggagaaga gcacgaacat ccccgcctcg gcctccggat ggcccgcgct
     1621 cacctcggcc agcacgatcg gggtcctggt ccacccggtc tccaccgccg ccgtgaactg
     1681 gacctcgacc cggccccgcc ggcacagacc gcgcagcgct tcgccgtccg cccggtgcac
     1741 cgagagcaga tggacgcggg ggagcgccgc gacggtctcc gccgacgggt tgccccagac
     1801 cggcgagcag cacatctcgt gcgcgtgccg gccgctgatg cagatcagcc ccagggcccc
     1861 ggcgcgggtc gcattgaccg cgtgctgcgg ggtggcccgc ccctccacca gcgccacctt
     1921 gccggcggcg cgagcggcgg cgtagtcgtc gggcgtcccc cggccggcgt acaccagttc
     1981 cgccgcaacg ccctccgggg gggtcgggat ccccatcgag tgcgtgatgc agaagatctc
     2041 ctgctgctgc gggcgcatca ggcgtagcga ggcggagcgg ggaaggctga tgtacgcatc
     2101 gtgcgtgagg atcctggtcg caagcccgaa tccgcgcaac tgttcctcgg cgtactcggc
     2161 ggcattccgt tcctccgcgc tgcccgagag gcgggtccac tgggcgatgg ccttgttgta
     2221 cgcgacgagc cgcgtctgtg agaccatatc gagatcgatc acgcaaatcg ccccccggca
     2281 gacgccgccg
//
LOCUS       DASQGH010000037         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19916, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000037 DASQGH010000000
VERSION     DASQGH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19916"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VEW91_00655"
     CDS             complement(<1..804)
                     /locus_tag="VEW91_00655"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HYM90125.1"
                     /translation="MAQEEKQAEVGVFGGSGFYTLLERPREIKVDTPYGEPSDAVMLG
                     EIAGRTVAFLPRHGRTHRLPPHRINYRANVWAMKSLGVGWVFGPCAAGSLHPRVRPGE
                     FVICDQFVDRTTGRADTFADGPIVTHISGAEPYCPTLRPLAVETARRLGIPVHERGTV
                     VVIQGPRFSTRAESRWFRTQGWEVINMTNYPECVLARELELCYVNISLITDYDVGVEG
                     DPAVQPVTQAAVLQVFRQNNENLRGLLLAMVERLPRKRTCSCATALAHAR"
     gene            complement(806..1597)
                     /locus_tag="VEW91_00660"
     CDS             complement(806..1597)
                     /locus_tag="VEW91_00660"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HYM90126.1"
                     /translation="MPAQTSTEIRRAAALLRRGRLNVVLTGAGVSTESGLPDFRSRDG
                     LWTRVDPARVASLSAFRRDPDAFYAFYATRLAALAQAAPNPAHRAIARLEALGVVHLV
                     VTQNVDGLHQQAGSRTVVEVHGNLREARCAACDALGPIAEMETMLRAGAVPRCRRCGG
                     LLRPNVVLFEELLPAAAYERAEAACRACEVLLVVGSSLEVYPVAGLPGLALEHGARLI
                     IVNREATPYDNLAALVVRGEAGAVLPEIAQAVQGMIEGRGRREEA"
     gene            complement(1606..2835)
                     /locus_tag="VEW91_00665"
     CDS             complement(1606..2835)
                     /locus_tag="VEW91_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90127.1"
                     /translation="MARVHQRFPRPRVEDVPDAVRAELRRLALGARIRPGGRIAVTAG
                     SRGIRDIVPILRAVVEEVRAVGGDPLLVAAMGSHGGGTDEGQRRLLQHLGITPESVGA
                     PLVTSMETVELGRTPSGFVAYCDRNAAACDAILAVSRIKPHTGFSQPFGSGVMKMLGV
                     GLGKAPGAAQIHQQGPAQMAGAIREIAERVIGTGRVLAGLAILENAYDETARVVGMPP
                     ERIPEVELELFPAARAMMPQLPVERMDLLIVDEVGKNYSGTGMDVNVIGRWRIAGLAE
                     PDAPHAERIVALRLSEASEGNAQGIGLADFTTRALVDQIDFTATYLNCIVSTYVQRAM
                     LPIVLATERDAVLAALGSLGLPDPLRARIVRVANTLHLEEVWASEPLVEGLRGRPHIA
                     EVGPLEPLRFDDRGRLC"
     gene            2974..>4157
                     /locus_tag="VEW91_00670"
     CDS             2974..>4157
                     /locus_tag="VEW91_00670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYM90128.1"
                     /translation="MSGRYSLFDLPRAFRSPGCVWFGAHASERLGEVLQDLGLRPGRA
                     LLVTDAVVDRLRLAEGAAAGRAAAGFDVHRFAEIPGEPSVEVADHAAAVARRLAPSLV
                     VGVGGGSVLDVAKLVAALVRNPGSALDYTHLVGRRFAETAVPTILVPTTAGTGAEASL
                     NAVVTTGDRKAFANKHPQLLAAGAILDPLLTHSLPPSVTAHTGLDALSHCMEGTLSTN
                     ATALTDLVAAQGTALIFGALRRACASGGSAAPDATARAQMVLAAYMGGLTLNAGMVLG
                     HSIAYTLANRLHLPHGLSCALALPYTLAYNRGAAPDRLAGLAGAAGVPGQDIVAHVGR
                     LCREVRIPDSIRALGLERDRLPELVDECLEQYPRPNNPRPLDRESLLSLYAAMWEGRP
                     AH"
BASE COUNT          575 a         1536 c         1449 g          597 t
ORIGIN      
        1 gcgggcgtgc gcgagcgccg tcgcgcagga gcacgtccgc ttgcggggta gccgctccac
       61 catggccagc aagaggcccc ggagattctc attgttctgc cgaaacacct ggagaaccgc
      121 ggcctgggtc acgggctgga cggcggggtc tccctcgacg cccacgtcgt agtccgtgat
      181 cagagagatg ttcacgtagc acagctccaa ctcccgggcc agcacgcact ccgggtagtt
      241 ggtcatgttg atgacctccc atccctgggt gcggaaccac cggctctccg cgcgggtgct
      301 gaaccggggc ccctggatga ccacgacggt gccgcgctcg tgcacgggga tcccgagcct
      361 gcgcgccgtc tccaccgcga gcggccgcag ggtcggacag tacggctcgg cccccgagat
      421 gtgtgtgacg atcgggccgt cggcgaaggt gtccgcgcgc ccggtggtcc ggtccacgaa
      481 ctggtcgcag atcacgaact cgcccggccg gacgcggggg tgcaggctcc ccgcggcgca
      541 ggggccgaac acccacccga ccccgaggga tttcatcgcc cacacgttcg cgcggtagtt
      601 gatgcggtgg gggggcagcc ggtgggtgcg gccgtgccgg gggagaaacg ccaccgtgcg
      661 ccccgcgatc tcgcccagca tcacggcatc gctgggctcc ccgtacggcg tgtccacctt
      721 gatctcgcgg gggcgttcca acaacgtgta gaagccggat cccccgaaca cccccacttc
      781 ggcctgcttt tcctcctggg ccacactatg cctcctcgcg tctgccccgg ccctcgatca
      841 tcccctggac ggcctgggcg atctccggga gcaccgcgcc agcctcgccg cgcaccacca
      901 gggcggccag gttgtcgtag ggggtcgcct cacggttcac gatgatgagc cgcgcgccat
      961 gctcgagggc caggccgggg agcccggcga ccgggtagac ctccagcgaa gatcctacca
     1021 ccagcaacac ctcgcacgcc cggcaggcgg cctccgcgcg ctcgtaggcc gcagcgggca
     1081 ggagttcctc gaacaggacc acgttcggac ggagcagccc tccgcaccgt cggcaccggg
     1141 ggaccgcccc ggcccgcagc atcgtctcca tctccgcaat cggtccaagc gcgtcgcagg
     1201 cggcgcaacg ggcctcgcgc agattgccgt ggacttccac gacggtgcgc gaaccggcct
     1261 gctggtgcag cccgtccacg ttctgcgtca ccaccaggtg caccactccc agggcttcga
     1321 gccgcgcgat cgcccggtgg gcggggttgg gggccgcctg ggccagcgcc gcaagacggg
     1381 tcgcgtagaa cgcgtagaag gcgtcgggat cccgccggaa cgcgctcagg gaagcgacac
     1441 gcgcggggtc cacgcgcgtc cacagcccgt ccctggagcg gaagtccggc agccccgact
     1501 ccgtgctgac cccggctccg gtcagcacga cattcagccg cccgcggcgg aggagcgcgg
     1561 ccgcccgcct gatctcggtg gacgtttggg cgggcaactg ctcgcctagc atagccgccc
     1621 gcggtcgtca aaccgcaggg gctctagagg cccgacttct gcgatgtgcg gccgcccgcg
     1681 caatccttcc accagcggtt ccgaggccca gacctcctcg aggtgcaagg tattcgccac
     1741 ccggacgatg cgcgcccgaa ggggatcggg gagcccgagg ctcccgagcg cggccaagac
     1801 cgcgtcgcgt tcggtggcca ggacgatcgg gagcatcgcc cgctggacat aggtgctgac
     1861 gatgcagttc agataggtgg ccgtgaagtc gatctgatcg accagcgccc gggtggtgaa
     1921 gtcggccagg ccgatcccct gcgcgttccc ctcggacgcc tccgacagcc ggagcgcgac
     1981 gatccgctcc gcgtgcgggg cgtcgggctc ggcgaggccg gcgatgcgcc accgaccgat
     2041 gacgttgacg tccatgcctg tcccgctgta gttcttgccc acctcgtcga cgatgagcag
     2101 gtccatccgc tcgacgggca gttggggcat catcgcgcgg gcggcgggga acagttcgag
     2161 ctcgacctcg gggatgcgtt cggggggcat cccgaccacc cgggccgtct cgtcgtaggc
     2221 gttctcgagg atcgcgagcc cggcgagcac gcgccctgtg ccgatcacgc gctccgcgat
     2281 ctcccggatc gccccggcca tctgggccgg gccctgctgg tggatctgcg cggcgccggg
     2341 ggctttcccc agcccgacgc cgagcatctt catcaccccg ctcccaaacg gctggctgaa
     2401 gccggtgtgg ggtttgatcc ggctgaccgc caggatcgcg tcgcacgccg ccgcgttccg
     2461 gtcgcagtac gccacaaacc cgctgggcgt ccgccccagc tccaccgtct ccatcgaggt
     2521 cacgagcggc gccccgacgg actcgggcgt gatgccgagg tgctgcagca gccgccgctg
     2581 gccctcgtcc gtgcccccac cgtggctccc catcgccgcc acgaggagcg gatctcctcc
     2641 aaccgcccgc acctcttcga cgacggcgcg caggatggga acgatgtcgc ggatcccacg
     2701 gctcccggcc gtaaccgcga tccggccgcc cggacggatt cgcgcgccca gcgcgaggcg
     2761 gcggagttcc gcgcggacgg catccggcac atcctccacg cgggggcggg gaaaccgctg
     2821 gtgcacccga gccatccggg gaaccgccgc gggaacgctg acgcctggag gaatcctctc
     2881 gacaccgatg ctcatggtgg ggaattgccc ggtgagccgc cccgatggta tcatctcggt
     2941 gaagtgcggg tcaacttgcc gagggagtca cagatgtccg ggcgctattc gctgttcgat
     3001 ctgccgcgcg cgtttcggag tcccggctgc gtctggttcg gcgcgcacgc gtcggaacgg
     3061 ctcggcgagg tcctgcagga cctgggcctc cggccggggc gcgcattgct cgtgaccgac
     3121 gcggtggtgg atcgtctcag gctcgccgag ggcgcggccg ccggccgggc ggccgccggg
     3181 tttgacgtcc accgcttcgc ggagatcccg ggggagccca gcgtcgaggt ggccgatcac
     3241 gcggcggcgg tggcgcggcg tctggcgccc tcgctcgtcg tcggcgtggg cgggggcagt
     3301 gtcctggatg tcgccaagct cgtcgccgcg ctggtgcgca acccggggag tgccctggac
     3361 tacacccacc tcgtggggcg gcggttcgcg gagaccgccg tcccgacgat cctggtgccg
     3421 accaccgcgg gcacgggggc cgaggcgagc ctgaacgccg tggtcaccac cggcgatcgg
     3481 aaggcgtttg ccaacaagca cccgcagctc ctcgccgcgg gcgcgatcct ggacccgctc
     3541 ctgacccact cgctcccgcc gtccgtgacc gcgcacaccg gcctggatgc cctgagccac
     3601 tgcatggagg gcacgctctc cacgaacgcg acggcgctca cggatctcgt ggcggcgcag
     3661 gggaccgcgc tgatcttcgg cgcgcttcgg cgcgcctgcg cgtcgggcgg atcggcggcc
     3721 ccggatgcca ccgcccgcgc ccagatggtc ctggccgcct acatgggcgg cttgacgctc
     3781 aacgccggca tggtcctcgg ccattcgatc gcgtacacgc tcgcgaaccg gctccacctg
     3841 ccccatgggt tatcgtgcgc cctcgcgctc ccatacacgc tggcctacaa tcggggcgcc
     3901 gccccggacc gcctcgctgg gctggcgggg gcggccgggg tcccgggcca ggacatcgtg
     3961 gcccacgtgg gccggctctg ccgcgaggtc aggatcccgg actcgattcg cgcgctcggc
     4021 ctcgaacggg accggcttcc cgaactggtc gacgagtgcc tcgagcagta cccgcgcccg
     4081 aacaaccccc ggccgctcga ccgggagtcc ctcctctcgc tgtatgccgc gatgtgggag
     4141 gggcgtccgg cccacga
//
LOCUS       DASQGH010000038         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_169, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000038 DASQGH010000000
VERSION     DASQGH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VEW91_00675"
     CDS             <1..548
                     /locus_tag="VEW91_00675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYM90129.1"
                     /translation="FPEVDVYSVFREIVTQDRRRATTGEGLGDVRTVTLAAAVLFRSL
                     TRRHFGTFPDVHRVLERLRTKYRLGLISNAQWVFTEPELAMADLDRFFPVRVLSSRVG
                     VKKPDPRLFTEAMNALGAAPEASLYVGDNPPRDLAGARNAGMRCVIFRGQDVPYNGLR
                     PDACFHVYADLEAIVDRLFGA"
     gene            608..1510
                     /locus_tag="VEW91_00680"
     CDS             608..1510
                     /locus_tag="VEW91_00680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90130.1"
                     /translation="MARVAFFGVGLMGEPMARHLLSAGHTVVLVAHRNRDGVQRLTAA
                     GAVEVSRAAEAVRRTEVAIMVLPTADDVEEVLFGGGQAAAELAPGYTVIDMGTSYPPH
                     TRRLAARVAERGGRFLDAPVTGGPKGAQEGTLTIMVGGDGATLESVRPLLAAMGSSIY
                     HFGGVGTGHTAKLVQNMIGIVTQAGIAEGFVLAAAAGLDVEQVFQMLASSMSASPALK
                     SMVPKVFARAFDQVNFRLDLAYKDIRQATQLAREMTVPLPAANGATELLQLARALGFG
                     AQDSTAVIRGLEAILRIEVKGGGH"
     gene            complement(1522..1923)
                     /locus_tag="VEW91_00685"
     CDS             complement(1522..1923)
                     /locus_tag="VEW91_00685"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HYM90131.1"
                     /translation="MTIDFSTPAYRVVWVYRPEANDYQRFLAGVPDTDAATGKFVYTK
                     SIAIAVIPRVHGRTRIGEDTWTFSDIGAGRAWMVQDGTVDEGRWQKPSRTDRLRFLSA
                     AGEEPALDRGRQWVEIVPPEVTPVIASAAPQ"
BASE COUNT          312 a          690 c          757 g          322 t
ORIGIN      
        1 gcttccccga ggtcgacgtc tactccgtgt ttcgggagat cgtgacccaa gaccgacggc
       61 gagccaccac gggcgagggc ctcggggatg tccgtaccgt gacgctcgcc gcggcggtcc
      121 tcttccgatc gctcacccga cggcacttcg ggacctttcc ggacgtccac cgcgtcctgg
      181 agcgccttcg gaccaagtac cggctcgggc tcatctccaa tgcccaatgg gtcttcacgg
      241 aacccgaact cgcgatggcc gacctcgacc ggttcttccc cgtcagggtg ctgtcgtccc
      301 gggtgggggt gaagaaaccc gatccccggc tgttcaccga ggcgatgaac gccctggggg
      361 cggcgccgga agcctccctc tatgtgggcg acaacccccc gcgagacctc gcgggggcac
      421 ggaatgccgg gatgcgctgc gtgatcttca ggggacagga tgtgccgtac aatgggctcc
      481 ggccggacgc ctgctttcat gtctacgccg acctggaagc gatcgtggat cgactgttcg
      541 gggcgtgatc gcccgacgat ccacgtcagt acgtgatcgg aacgatgatc gcccaggagg
      601 agacgccatg gcgcgggttg cgttcttcgg ggtcgggctg atgggcgagc cgatggcgag
      661 gcacctcctg tcggcgggac acaccgtggt gctggtcgcg caccgtaacc gcgacggggt
      721 gcagcgcctg acggccgccg gggccgtgga ggtgtcgcgg gcggcggagg ccgtgcgtcg
      781 caccgaggtg gcgatcatgg tgctgccgac ggcagacgac gtcgaagagg tgctcttcgg
      841 cggcgggcag gcagccgccg agttggcgcc cgggtacacc gtgatcgaca tgggaacgag
      901 ttacccgccc cacacccgcc ggctggccgc gcgggtggcg gagcggggcg gccggtttct
      961 cgacgcccca gtgaccgggg gaccaaaagg cgcccaagag gggaccctga cgatcatggt
     1021 cgggggcgac ggggcgacgc tggagtcggt gcggccgctg ctcgcggcga tggggtcatc
     1081 gatctaccat ttcggcgggg tcggaaccgg ccataccgcg aagctcgttc agaacatgat
     1141 cgggatcgtg acccaggcgg ggatcgccga agggttcgtg ctggccgcgg ccgcggggtt
     1201 ggacgtcgag caggtctttc agatgctcgc gtccagcatg tcggcgagcc ccgcgctcaa
     1261 gtccatggtg cccaaagtgt tcgcgcgcgc gttcgaccag gtcaacttcc ggctcgacct
     1321 ggcgtacaag gacatccgcc aggcgaccca gttggcgcgg gagatgacgg tgccgctgcc
     1381 ggcggccaac ggcgcgaccg aactgcttca gctagcgcgc gcgctcggct tcggcgcaca
     1441 ggacagcacg gcggtgatcc gggggctcga ggccattctc cggatcgaag tcaaaggcgg
     1501 cggccactag ccgatctcgt cctactgcgg cgccgcggag gcaatcaccg gggtgacctc
     1561 gggcggaacg atctcgaccc actgccggcc gcgatcgagc gccggctcct cgcccgcggc
     1621 gctgaggaag cggagccggt cggtgcggga gggcttctgc cagcgccctt cgtccaccgt
     1681 cccatcctgg accatccacg cccttcccgc accgatgtcg gagaaggtcc aggtgtcctc
     1741 cccgatccgg gtccgcccgt ggacgcgcgg gatcaccgcg atcgcgatcg acttcgtata
     1801 gacgaacttc cccgtcgccg cgtcggtatc cggaaccccc gccaggaacc gctggtagtc
     1861 gttggcttcg ggccggtaga cccacacgac gcggtaggcg ggggtggaga agtcgatcgt
     1921 caccgtctgg ccgggcggcc gggtggttct cgatcacgac ggcgagcggc cggcgggcgg
     1981 caagggcctc gggcaccggc tcaccatcga ccgggtcgtc cacgaactcg gggtgggagg
     2041 actcctgtgc gacttccggc gcgggtgctg gcgcgactgg c
//
LOCUS       DASQGH010000039         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11466, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000039 DASQGH010000000
VERSION     DASQGH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11466"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VEW91_00690"
     CDS             complement(<1..823)
                     /locus_tag="VEW91_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90132.1"
                     /translation="MRSVRFLSAGALVCASLALVSGLSAPASWGAPAAPGCTVSVPVL
                     VVGGTPAGVAAAVSAARLGSRVMLIEPRPYLGGDLTGAMLNMFDMDFGPGGMHLARGV
                     FTDIYQQLGMTFDVELAKLVFLREIRQESRVTLRLLTRPGGVVLDGPQIVGVEVKDLQ
                     TREHQTICAKRVVDATDDADIAAMAGVPYLTGRQGSGIDRAMMAATLVFELDGVNWRE
                     VVSYATGRGPRGLRGGTYHGNVWGYGEIMRGYRPTTPGVGIYDLNIGRQNDRSVLV"
     gene            complement(1047..1781)
                     /gene="tatC"
                     /locus_tag="VEW91_00695"
     CDS             complement(1047..1781)
                     /gene="tatC"
                     /locus_tag="VEW91_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYM90133.1"
                     /translation="MDRDKHMTLVEHLEELRQRLQYAVLGLLVATGVGLVFVDLLLRI
                     LLRPAGNVKLTTLTVLEPFLVKVKVAFLFGFAVSMPWIMYQLYAFVDPALTDTERRRT
                     IPLALLAGLLFACGIVFGYLLILPVSLHWLLAQAGTQFNALITANAYISFVLFFLLIV
                     GATFETPLVILTLAFLGVVSPQTLRREWRIAYMVIAGIAVFGTPDWSPVTMLLVAIPM
                     AVLYEFSIVLCRIFIRPPAPQPVRET"
BASE COUNT          338 a          666 c          631 g          259 t
ORIGIN      
        1 tcaccagcac cgaccgatcg ttctgccgcc cgatattcag gtcgtagatg ccgacccccg
       61 gagtggtggg ccgatacccc cgcatgatct ccccgtatcc ccagacgttg ccgtggtagg
      121 ttccgccccg caggccccgg gggccccggc cggtggcgta cgacaccact tcccgccagt
      181 tgaccccgtc cagctcgaag accagggtcg ccgccatcat cgcgcggtcg attcccgatc
      241 cttggcgacc cgtcagatac ggcacccccg ccatcgcggc gatgtcggcg tcgtcggtgg
      301 cgtcgacgac ccgcttggcg cagatcgtct gatgctcgcg cgtctggagg tccttcacct
      361 cgaccccgac gatttgcggg ccgtccagca ccaccccgcc cggccgggtc agcaaccgca
      421 gggtcacccg ggactcctgg cggatctccc gcaagaaaac gagcttggcc agttcgacgt
      481 cgaaggtcat cccgagttgc tggtagatgt ccgtgaagac gccccgcgcg aggtgcattc
      541 cgccggggcc gaagtccatg tcgaacatgt tgagcatcgc gccggtgaga tcgccgccga
      601 gatacggccg gggctcgatg agcatgaccc gggatcccag ccgcgccgcc gagaccgcgg
      661 cggccacgcc ggcgggggtc ccgcccacca ccagcacggg gacggacacc gtgcaccccg
      721 gcgcggcggg ggcgccccac gaggcaggcg ccgaaagccc tgaaacgagc gccaaggagg
      781 cgcaaacgag cgccccggcg ctgaggaacc tgaccgaccg catccctcgc cgcatctctt
      841 atcgccctat tataacaaac cctcccgtcg ggggcactcc gcccttcgga tacggagcgg
      901 ccgcgctcgg atgacccgac ccccgccgtc cagatggagg cctcccacct ccccgtacgg
      961 ccacgggggc agcggggttc ggcgcgggag ggggaggaac ccgggtagcc gggccccgga
     1021 ggggcccgcg tccggcggct cggtctctag gtttcgcgaa ccggctgcgg cgccggcggc
     1081 cggataaaga tgcggcagag cacgatgctg aactcgtaga gcaccgccat cggaatcgcc
     1141 accaggagca tcgtcaccgg gctccagtcg ggggtgccga acaccgcgat ccccgcgatg
     1201 accatgtaag cgatgcgcca ctcgcggcgc agggtttggg gggacacgac gccgaggaag
     1261 gcgagggtga ggatcacgag cggcgtttcg aacgtcgcgc cgacgatcag gaggaagaac
     1321 agcacgaacg agatgtaggc gttggcggtg atcagcgcgt tgaactgggt gcccgcctgg
     1381 gcgagcagcc agtggaggct caccggaaga atcagcaggt agccgaacac gatgccgcac
     1441 gcaaacagca gcccggcgag gagcgccagc gggatcgttc tccgccgctc ggtgtccgtg
     1501 agcgccgggt cgacgaacgc gtagagctgg tacatgatcc acggcatgct caccgcgaat
     1561 ccgaacagaa acgccacctt caccttcacc aggaacggct cgagcacggt gagcgtggtc
     1621 agcttcacat tcccggccgg ccggaggagg atccggagca ggaggtcgac gaacaccagc
     1681 cccacgccgg tcgccaccag cagcccgagg accgcgtact gaagccgctg gcggagctcc
     1741 tcgaggtgct ccaccagcgt catgtgcttg tcccgatcca tcatcctact ccgtgccgcg
     1801 cgcgagctct gaaaccgtca cgagcgcgta accgcggtgc tgcagttctc cgatgattgc
     1861 gggcagcgcg cggacggtat tgagcgtccc atcg
//
LOCUS       DASQGH010000040         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22774, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000040 DASQGH010000000
VERSION     DASQGH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22774"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VEW91_00700"
     CDS             complement(<1..536)
                     /locus_tag="VEW91_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYM90134.1"
                     /translation="MLTLEKLTPPREGQRIEVRDQRLYVPDTPIIPFIEGDGTGPDIW
                     RATVRVLEAAVRKAYGARRQIVWFEVFAGEKAHTRFGTWLLDDTLRAFEYYTVGIKGP
                     LTTPIGGGFRSLNVSLRQQLDLYACVRPVRYFEGVPSPVRHPEQVDVVVFRENTEDIY
                     VGYELAGGTPEARRLIEFV"
     gene            complement(541..1293)
                     /locus_tag="VEW91_00705"
     CDS             complement(541..1293)
                     /locus_tag="VEW91_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HYM90135.1"
                     /translation="MAEATFQVFRGDARGGDLVTYPVPVTEGMVVLDGIHYIQAHLDG
                     TLACRWNCKAAKCGSCSAEVNGRPRLMCKTRVDELGDTPITVRPIKVFAMIKDLVTDV
                     SWNYRVNQQIPPFAPPPNDPGPWKLYPEDVERLYEFRKCIECFLCQDVCHVLREHDGM
                     ERYMGPRFMVRIAALEMHPKDTRSRTDLLKGRGGIGLCNITKCCEEVCPEHIHITDNA
                     IIPLKERMADDYFDPWRAFLRMFGRKKEPRGS"
     gene            complement(1311..>1892)
                     /locus_tag="VEW91_00710"
     CDS             complement(1311..>1892)
                     /locus_tag="VEW91_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HYM90136.1"
                     /translation="LGANRLGGNSLSDLLVFGKRAGEHAAAYVRGVGTAPRVDDRQAD
                     EERRLLQRPFDGGGRENPFAMHEELQEVMGTYAGIARTGAQLEEGLQKVRDLQTRAAH
                     LGAAGSRLFNPGWLTCRDVQFMLTVCEAIFRSAIERKESRGAHWRLDFPDQDPAWGAQ
                     NIIVSKGDGGMQVRTRPVPQMPAELARLVRPGG"
BASE COUNT          281 a          682 c          607 g          322 t
ORIGIN      
        1 acgaactcga tcagacgcct ggcctcgggc gtcccgccgg cgagctcgta tccgacgtag
       61 atgtcttcgg tgttctcgcg gaacaccacc acgtccacct gttccgggtg ccggaccggg
      121 ctcggcacgc cctcgaagta ccgcaccggg cggacgcacg cgtagaggtc gagctgctgg
      181 cggagcgaga cgttcaggct gcggaatcct ccgccgatcg gggtggtaag cgggcccttg
      241 atccccacgg tgtaatactc gaacgcacgg agcgtgtcgt cgagcaacca cgtgccgaac
      301 cgcgtgtgcg ccttctcgcc cgcaaagacc tcgaaccaga cgatctgcct ccgcgccccg
      361 taggccttcc tgaccgccgc ctccaggacg cgcacggtcg cacgccagat atccggcccg
      421 gtgccgtcgc cttcgatgaa cgggatgatg ggggtgtcgg gcacgtagag ccgctggtcg
      481 cgcacctcga tccgctgccc ttcgcgcggc ggggtaagct tctcgagcgt cagcatggcg
      541 ctagctccct cgcggctcct tcttgcgtcc aaacatcctg aggaacgccc tccacggatc
      601 gaagtagtcg tccgccatcc gctccttgag cgggatgatg gcgttgtcgg tgatgtggat
      661 gtgctcgggg cagacctcct cgcagcactt ggtgatgttg cacagcccga tgccgccgcg
      721 ccccttgagc aggtccgtcc tcgagcgggt gtccttgggg tgcatctcca gggccgcgat
      781 ccggaccatg aaccggggcc ccatgtaccg ttccatcccg tcgtgttccc gcaggacgtg
      841 acagacgtcc tggcagagga agcactcgat gcacttccgg aactcgtaca atcgttccac
      901 atcctccgga tacagcttcc aggggccggg gtcgttcggg ggcggggcaa acggcgggat
      961 ctgctggttg acccggtagt tccacgagac atcggtcacg aggtccttga tcatcgcgaa
     1021 gaccttgatc ggacggacgg tgatcggcgt gtctcccagt tcgtcgaccc gggtcttgca
     1081 catcagccgc gggcggccgt tcacctccgc gctgcaggac ccgcacttcg ccgccttgca
     1141 attccagcgg cacgccaggg ttccgtccag gtgggcctgg atgtagtgga tgccgtcgag
     1201 gacaaccatc ccctcggtaa ccggcaccgg gtaggtgacc aagtcccccc cgcgggcgtc
     1261 gccccggaac acctggaacg ttgcctccgc catcgctccc tcccccgccg ctacccgccc
     1321 ggccgcacca gccgggcgag ctccgccggc atctgcggga ccggccgggt ccggacctgc
     1381 atcccgccgt cgcccttgct gacgatgatg ttctgagcgc cccatgccgg atcctgatcg
     1441 ggaaagtcga gccgccagtg cgcgccgcgg ctctccttcc gctcgatcgc cgaccgaaag
     1501 atcgcctcgc acacggtcag catgaactgg acgtcacggc aggtgagcca gccggggttg
     1561 aagaggcgcg atcccgccgc cccgaggtgg gccgcgcggg tctggaggtc gcggaccttc
     1621 tggagcccct cctccagttg ggcgcccgtc cgggcgatcc cggcataggt ccccatcacc
     1681 tcctgcagtt cctcgtgcat cgcaaagggg ttctcccgcc cccctccgtc gaacggacgc
     1741 tggagcagcc ggcgctcttc gtcggcctgc cggtcgtcga cccggggcgc ggtccccact
     1801 ccccgtacgt aggccgccgc gtgctccccg gcgcgcttgc cgaagaccag gaggtcggag
     1861 agggagttgc cgcccagccg gttcgcccca ag
//
LOCUS       DASQGH010000041         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39779, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000041 DASQGH010000000
VERSION     DASQGH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39779"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VEW91_00715"
     CDS             complement(<1..576)
                     /locus_tag="VEW91_00715"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90137.1"
                     /translation="MDRRTFLRRSAETAAGAALLAAGASWTAREPGTAVAAATPAVRA
                     AVQFGWVKTVEFAGSFIADNEGYYKAEGLEIEMLSGGPGIDVVTVVVSGKALFGCGTG
                     AQILARARQRGAPIKVIGVLYQKSPLAVLSLKKNPIRTPQELLGKRVGVAAADSIPWE
                     TWLALHHLDASKLTRVPVQFDPAPLVTGDVDA"
     gene            708..1103
                     /locus_tag="VEW91_00720"
     CDS             708..1103
                     /locus_tag="VEW91_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYM90138.1"
                     /translation="MARDAASIDTREQKRDDHLRSPDFLDVARYPALTFRSTAILPKE
                     NGRYDVKGELTIRGTSRPVSFEIEVTDPIKDPFGNLRAAATGEGRLNRKEWGLTWNQT
                     LEFGALMVGEEIRFRVEVEAVARTPVAVG"
     gene            1127..>1922
                     /locus_tag="VEW91_00725"
     CDS             1127..>1922
                     /locus_tag="VEW91_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYM90139.1"
                     /translation="MNTKAGPGRSLGYRMPAEARLTSLTLRVRDLARLQRFYADLLGL
                     HVQPGGPDHIGVGPEGKAFALELVQDPQAPVRPHPSVGLYHFALLLPDRPQLAAVVRR
                     LMEAEWPFEGASDHGVSEALYLRDPEGNGIELYRDRPDGEWPHRNGELAMVSQALDVS
                     AQLAEAPTSAPIHPATRLGHIHLSVDDLHAGEQFYSTILGLNVTQRSYPGALFLAAGT
                     YHHHVGLNTWAHGRRAPGNATGLTGYAWAVPRGTVDALSGHLGTAGV"
BASE COUNT          314 a          666 c          640 g          302 t
ORIGIN      
        1 cgcgtccacg tcgccggtga ccagcggggc cgggtcgaac tggaccggaa ccctcgtgag
       61 cttggaggcg tccaggtggt gcagcgccag ccaggtttcc caggggatgc tgtcggcggc
      121 ggcgaccccg accctcttgc ccagcagttc ctggggggtg cggatcggat tcttcttaag
      181 ggagaggacc gccagggggc tcttctggta gaggaccccg atgaccttga tcggagcgcc
      241 ccgctgtcgc gccctcgcca ggatctgcgc gcctgtcccg cagccgaaca aggctttgcc
      301 tgagacgacc accgtgacga catcaatgcc cgggccgccg gagagcatct cgatctcgag
      361 accctccgcc ttgtagtagc cctcattgtc ggcaatgaag cttccggcga attccactgt
      421 cttcacccag ccaaactgga cggcggccct caccgcgggc gtcgccgcgg cgaccgccgt
      481 gccaggctcc cgcgcagtcc aggaggcccc cgccgcgagc agcgctgctc ccgcagccgt
      541 ttcggcgctc cgccggagaa acgtccggcg atccatagag agcgcgtccg gccgatgtct
      601 caaccccgca cctccacgca ttcggcggcc tacgccgttc ttctcaacgc cctgcaacat
      661 gcccgcactc ttcatcacca tgcggaggtt tcctgcctcg tgatgctgtg gcgcgtgacg
      721 ccgccagcat cgacacccgc gagcagaagc gggacgacca tctcaggtcc cccgacttcc
      781 tggacgttgc tcggtatcca gcgctgacat tccggagcac cgcgattctc ccgaaggaga
      841 acgggcggta cgacgtgaag ggggagttga cgatccgcgg aacgtcccgc ccggtgagct
      901 tcgagatcga agtcacggac ccgatcaagg acccgttcgg caacctccgg gccgccgcca
      961 cgggcgaggg gaggctcaac cgcaaggagt ggggcctcac gtggaaccag acgctggagt
     1021 tcggcgccct catggtgggt gaagagatac ggttccgggt cgaggtcgag gcggtggcaa
     1081 ggacgccggt cgcggtcggc taacggccgg aaggagcagc gcggtggtga acaccaaagc
     1141 gggaccggga aggtcgctcg gctatcgcat gcctgctgag gcccgcctca cgagcctcac
     1201 ccttcgggtg cgggacctcg cccggctgca gcgcttttac gccgatctcc tggggcttca
     1261 cgtccagccc ggaggtcccg atcacattgg agtggggccg gaggggaagg ccttcgcgct
     1321 cgagctcgtc caggacccac aagcgcccgt gcgcccccac ccgtcggtcg ggctctacca
     1381 cttcgcgctg ttgttgccgg accgcccaca gctggcagcc gtggtgcggc gcctcatgga
     1441 ggctgagtgg ccgtttgaag gcgcaagcga tcacggcgtc tccgaggcgc tctatttgag
     1501 agatcccgag ggaaacggca tcgagctcta ccgcgatcgc cccgacggcg agtggccgca
     1561 tcgcaacggg gaactggcga tggtcagtca ggcgctcgac gtctcggccc agctggcaga
     1621 ggcccccacc tcggccccca tccatccggc gacgcggctg gggcacatcc acctttccgt
     1681 cgatgacctg cacgcaggag aacagttcta ctcgaccatc ctgggactga acgtcaccca
     1741 gcgctcgtat cccggggcgc tctttctcgc cgcgggaacc tatcaccacc acgtggggct
     1801 gaatacctgg gcgcacgggc gacgagcgcc gggcaatgcg accgggttga ccgggtacgc
     1861 ttgggcggtc ccgcgaggga cggtggatgc cctgtccggg cacctcggaa cggcgggcgt
     1921 cc
//
LOCUS       DASQGH010000042         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17168, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000042 DASQGH010000000
VERSION     DASQGH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17168"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VEW91_00730"
     CDS             <1..240
                     /locus_tag="VEW91_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90140.1"
                     /translation="MDYTEYGGAPLLGVNGICIIAHGSSKAKAIRNSIALAAESVRAR
                     MVETIRDDIARLSSLPPLSPLDAAELPVSRSPADS"
     gene            299..1303
                     /locus_tag="VEW91_00735"
     CDS             299..1303
                     /locus_tag="VEW91_00735"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYM90141.1"
                     /translation="MRTGSTIVGLGRYLPERILTNAELARAVDTTDEWIRSRTGVAER
                     RIAAPAEASSDLAYGAAREAMEAAGIGPSEIDLIVVGTTTPDMVFPNVACLLQKRLGT
                     RTVGCLDVSAACSSFVYGLSVAHGAIASGQAETVLVVGAETLSRITNWRDRATCVLFG
                     DCAGAAVLRPARRGSGFLSFCLGGDGQGETALYLKAGGSGQPASYETVERGEHYISMN
                     GPEVYQFAVRAITDAAAQALREADLGPADVDFVIPHQANIRIIESAARRLRIPLDKFF
                     VNVQRYGNTSAASIPVALYEAVEAGHVGDGRVGVLVTFGAGYTWAACAIRWGGGQERR
                     "
     gene            1300..>2167
                     /gene="fabD"
                     /locus_tag="VEW91_00740"
     CDS             1300..>2167
                     /gene="fabD"
                     /locus_tag="VEW91_00740"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYM90142.1"
                     /translation="MSAGFAAVFPGQGAQHVGMGCEIAERFPEARRVFERASAVAGFD
                     VLGLCAAGPEEALRATVNTQPAILVTSLACLATLPATPQIAAGLSLGEYTALVCAGAL
                     LVEDAVRLVRLRGIYMEEATRGRETLMVAVIGLSPDQARALCAAHAHLGVVEPANFNC
                     PGQIVIGGDAPAVRAAAAAARAMGARRSVPLPVSAPFHTSLMRPAAERLAVELERTSI
                     RPAQIPVVANASAQPVRQPEEIRRALLAQVASPVLWEQSVRALFDSGVRQFVEIGPGT
                     TLSGMIRKTVSAE"
BASE COUNT          285 a          760 c          816 g          306 t
ORIGIN      
        1 atggactaca cggagtacgg cggcgcccca ctcttggggg tcaacgggat ctgcatcatc
       61 gcccacggaa gctccaaggc gaaggcgatc cggaactcca tcgcgctggc cgccgagtcg
      121 gtccgcgccc gcatggtgga gacgatccgg gacgacatcg cgcgcctgtc ctccctgccg
      181 ccgctttccc ccctggacgc ggccgagctt cccgtctccc ggtctcccgc cgactcgtga
      241 tcacgcccgg caccacacgc gcagtggcag tacacgccaa cgcctaaggg ggcgccaggt
      301 gcgcacagga tccaccatcg tcgggttggg ccgatacctg ccggagcgaa tcctgaccaa
      361 cgccgaactg gcgcgcgcgg tcgacacgac cgatgaatgg atccggtcgc gcaccggcgt
      421 ggccgagcgg cggatcgccg cccccgcgga ggccagttcc gatctggcct acggcgccgc
      481 gcgggaggcg atggaggcgg cggggatcgg tccctccgag atcgacctca tcgtcgtcgg
      541 gaccaccacc ccggacatgg tctttccgaa cgtcgcgtgc ctgctccaga agcggctggg
      601 gacccggacc gtgggctgcc tcgacgtctc ggcggcctgc tcgagcttcg tctacgggct
      661 gagcgtcgcc cacggggcga tcgcgtccgg ccaggccgaa acggtgctgg tggtcggggc
      721 ggagaccctc tcgcggatca ccaactggcg tgaccgtgcc acctgcgtgc tgttcggcga
      781 ctgcgcgggg gcggccgtcc tgcggcccgc ccggcggggc agcgggttcc tgtcgttttg
      841 cctgggcggg gacgggcagg gcgaaacggc cctgtatctg aaagcgggcg ggagcgggca
      901 gccggcctcc tacgagacgg tcgagcgggg ggagcactac atctccatga acggtcccga
      961 ggtctaccag ttcgccgtgc gggcgatcac cgacgcggcc gcccaggcgc tccgggaggc
     1021 cgatctcggg cccgcggacg tcgacttcgt gatcccgcac caggccaaca tccggatcat
     1081 cgagtccgcc gcccgccggc tgcggatccc cctggacaag ttcttcgtca acgtccagcg
     1141 gtacggcaac acgtctgcgg cctcgatccc ggtggcgctg tacgaggcgg tggaagccgg
     1201 acacgtcggg gacggccggg tcggcgtgct ggtgaccttc ggcgccgggt acacgtgggc
     1261 ggcgtgcgcg atccggtggg gcgggggcca ggagcggcga tgagcgccgg gttcgcggcg
     1321 gtctttccgg ggcagggcgc gcaacacgtc ggcatgggct gcgagatcgc cgagcgcttt
     1381 cccgaggccc ggcgggtctt tgagcgcgcc agcgccgtgg cgggattcga cgtgctcggc
     1441 ctgtgcgccg cgggcccgga ggaagccctc cgggccactg tgaacacgca gccggcgatc
     1501 ctggtcacga gcctcgcctg cctcgcgacg ctgcccgcca ccccgcagat cgccgcgggg
     1561 ctgagcctgg gggagtacac ggcactggtg tgcgccggcg cgctcctcgt ggaggacgcg
     1621 gtcaggctgg tgcggctccg gggcatctac atggaggagg ccacgcgggg acgggagacg
     1681 ctgatggtcg cggtgatcgg gctgagcccg gaccaggccc gcgcgctgtg cgcggcgcac
     1741 gcccacctgg gcgtggtcga gccggcgaat ttcaactgcc cgggccagat cgtgatcggc
     1801 ggggatgccc ccgcggtgcg ggcggccgcc gccgccgccc gggcgatggg cgcccggcgc
     1861 tcggtgccgc tcccggtgag cgcgccgttt cacacgagcc tgatgcggcc ggccgcggag
     1921 cggctcgccg tcgagctgga gcggacctcg atccggcccg cgcagatccc cgtcgtggcc
     1981 aatgcgtcgg cgcaacctgt gcggcagccg gaggagatcc gccgcgcgct cctggcccag
     2041 gtggcgtccc cggtcctctg ggagcagtcg gtgcgagcgc tcttcgactc cggggtgcgg
     2101 cagtttgtcg agatcgggcc cgggaccacg ctctccggga tgatccgcaa gacggtgtcg
     2161 gccgaga
//
LOCUS       DASQGH010000043         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51111, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000043 DASQGH010000000
VERSION     DASQGH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51111"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3506)
                     /gene="gltB"
                     /locus_tag="VEW91_00745"
     CDS             complement(<1..>3506)
                     /gene="gltB"
                     /locus_tag="VEW91_00745"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HYM90143.1"
                     /translation="VLDNVLELVTQSGRDLLHAMMMLVPEAWENVAEMPEDLRAFYRF
                     HAGLAEPWDGPAALAFSDGRVAAAALDRNGLRPARYTIMDDGLVIVASVVGVIALDPA
                     RIVEKGRLGPGRMIAVDTAAGRILTNDAIKEERARRRPYATWVSTGRLRLEAGAAPTG
                     ARPAVDGTGGGKRLGGGDLRRELAAFGYSQEELQRILQPMWKDAVEPVWSMGDDTPLA
                     VLSARPRLLYNYVRQRFAQVTNPPIDSLRERLVMSVRTLLGGRGNFLEESPEHARLIE
                     LESPLLSNAQLADLCALPGYRARALAAVFPTAEDGEGLERALVRLCDEAVYHVEEGTT
                     LLVLSDRSTDPARAPIPMLLAVGAVHQELIRRGLRMRVSLVAETGEARDIHHIAALIG
                     YGASAVNPYLVYEVIAEEAARAGAEPTDALARYRKAIESGLLKVMSKMGISTVSSYHG
                     AQIFEAVGLDHALVEFALTGTPSRLGGIGLGEIAEDVLERHRRAFGGSPPTSLGDPGL
                     FRFRKDGEYHAFHPNVVRTLHRLALSGSQEDYLAYAWEVAHRPPTAIRDLLEFRPGAA
                     IPVEEVEPAETIARRFVVSSMSHGSLSKEAHETLAIAMNRLGAKSSSGEGGEDPARYR
                     PRPNGDWANCAVKQVASGRFGVTTEYLAAAQELEIKMAQGSKPGEGGQIPGSKVTEEI
                     ARIRRAQPGISLISPPPHHDIYSIEDLAQLIYDLKQGNPRARVSVKLVSEAGVGTIAA
                     GVAKAYADTVHIAGCDGGTGASPLDSIKNAGVPWELGLAETQQTLVANNLRGRVRVRV
                     DGGMKTARDVVIAAMLGAEEFGFGSAAVVALGCVMARQCHLNTCPVGIATQQHDLRAK
                     FPGTPERVVHFFLAIAHDVRRILAALGFRAVAEVVGRADLLRVRGDIPAPRARRLVLD
                     AILADADPSGTRPRRHLHERNDRPGEPYDDRILVEIGEDLVAGRPVDRTFPIRNIDRT
                     VGARVASAITRKSGDAGLPDGAITLRFVGSAGQSFGAWCVGGMRLGLLGEANDYVGKG
                     MTGGEIVIAPPEPLRAASCRHVIMGNTVLYGATGGRLFAAGSAGERFAVRNSGAIAVV
                     EGVGDHGCEYMTGGIVVVLGETGRNFGAGMTGGAAYIADEQETLERRLNPALVTLGPV
                     EDAGDAAQLRALVTAHWEATGS"
BASE COUNT          497 a         1333 c         1156 g          520 t
ORIGIN      
        1 gctccccgtc gcctcccagt gcgccgtcac aagcgcgcgc agttgcgcgg catcaccggc
       61 atcctccacg gggccgagcg tgaccagtgc cgggttgagc cggcgctcca gcgtctcctg
      121 ctcgtcggcg atgtacgctg cccctccggt catgccggct ccaaaattgc gcccggtctc
      181 cccgagcacc acgacgatcc cgccggtcat gtactcgcag ccgtgatcgc cgaccccttc
      241 caccaccgcg atggccccgc tgttccggac cgcgaatcgc tccccggcgc tccccgcggc
      301 aaagagccgg ccgccggtgg cgccgtagag cacggtgttg cccatgataa cgtggcggca
      361 cgacgccgcc ctcaggggct cgggcggcgc gatgacgatc tctccacccg tcatcccctt
      421 accgacatag tcgttggcct cgccgagcag ccccagccgc attcccccca cgcaccatgc
      481 gccaaacgac tgccccgcgc tgccgacgaa ccgcagggtg atcgcgccgt ccggcaaccc
      541 ggcgtcgccg gacttccggg tgatcgcgct cgcgacccgg gcgccgacgg tccggtcgat
      601 gttccggatc ggaaacgtcc ggtcgaccgg gcggccggcc acgagatcct ccccgatctc
      661 gacgaggatg cgatcgtcgt acggctcccc cgggcgatcg ttgcgctcgt gcaggtgacg
      721 cctcggtcgg gtccccgagg gatccgcatc ggccaggata gcatcgagca cgagccgtct
      781 ggcccgaggc gccgggatat ccccccgcac gcgcagaagg tcggcccgcc cgacgacctc
      841 cgcaaccgcc cgaaagccga gcgcggcgag aatccgacgg acgtcgtggg cgatcgccag
      901 gaagaagtgg acgacccgct cgggggtccc gggaaacttc gcccggaggt cgtgctgctg
      961 ggtcgcgatg cctaccgggc aggtattgag atggcactgc cgcgccatca cacagccgag
     1021 ggccaccacc gccgcgctcc caaacccgaa ctcctcggcc cccagcatcg cggcgatcac
     1081 gacgtcccgg gccgtcttca tcccgccgtc gacccggacc cggacccggc ccctgaggtt
     1141 gttcgcgacc agcgtctgct gggtctcggc cagccccagc tcccacggca cccccgcgtt
     1201 cttgatcgag tccagcggcg aggcgccggt gcccccgtcg catccggcga tgtggacggt
     1261 gtcggcgtag gccttcgcga cccctgccgc gatcgtcccg acgccggctt cgctgacgag
     1321 tttgacggag acccgcgccc gggggttgcc ctgcttgaga tcgtagatca gctgggccag
     1381 atcctcgatg ctgtagatgt cgtggtgggg cggaggggag atcagcgaga tccccggctg
     1441 ggcccggcgg atgcgtgcga tctcctccgt caccttcgac ccggggatct ggcccccctc
     1501 accgggcttg ctgccctgcg ccatcttgat ctcgagttcc tgcgccgccg ccagatactc
     1561 cgtcgtcacc ccgaaccgac ccgatgccac ctgcttgacc gcgcagttcg cccagtcccc
     1621 gttggggcgc ggtcggtatc gggccgggtc ctccccgcct tcgccgctgc tgctcttcgc
     1681 gccgagccgg ttcatcgcga tcgccagcgt ctcgtgcgcc tccttgctca gggagccgtg
     1741 cgacatcgac gagacaacaa accgccgggc gatcgtctcc gcgggctcaa cctcctccac
     1801 cgggatcgcg gcccccggcc tgaactccag gaggtcccgg atcgccgtcg gcggccggtg
     1861 ggccacctcc cacgcgtagg ccaggtagtc ctcctggctg ccgctgaggg ccagccggtg
     1921 cagggtgcgc acgacgttgg gatggaaggc gtgatactcc ccgtcctttc ggaaccggaa
     1981 cagcccagga tcgccgagcg aggtgggcgg cgacccgcca aatgcccgcc ggtgacgctc
     2041 gagcacgtcc tccgcgattt ctccgaggcc gatccctccg aggcgcgagg gcgtgccggt
     2101 gagggcgaac tccacgagcg cgtggtccag ccccacggcc tcgaagatct gggcgccgtg
     2161 atagctgctg accgtggaga tgcccatctt ggacatcacc ttgaggagcc ccgactcgat
     2221 cgccttacgg taccttgcga gcgcgtcggt tggctccgct ccggcccggg ccgcctcctc
     2281 cgcgatcacc tcgtacacga gatacgggtt gaccgcgctg gccccgtagc cgatcaacgc
     2341 cgcgatgtgg tgaatgtccc gcgcctcccc ggtctccgcc accaggctca cccgcatccg
     2401 caggccccgg cggatgagct cctggtgcac cgcgcccacg gcgagcagca tcgggatcgg
     2461 cgcgcgcgcc ggatcggtgg agcggtcgga gaggacgagc agcgtggtcc cttcctccac
     2521 gtggtagaca gcctcgtcac acagccggac cagcgcccgc tcgagccctt ccccgtcctc
     2581 ggcggtgggg aaaaccgcgg ccagggcgcg cgcccgatac cccgggaggg cgcagaggtc
     2641 cgccagttgg gcgttcgaca agagcgggct ctcgagttcg atcaagcgcg cgtgctcggg
     2701 gctttcttcg aggaagttcc cgcggccccc caggagcgtc cgcacggaca tgaccagccg
     2761 ctcgcggagc gagtcgatcg gcggattggt gacctgcgcg aaccgttgcc tgacgtaatt
     2821 gtacaggagg cgcggccggg ccgagaggac cgcgagcggg gtgtcgtccc ccatggacca
     2881 gaccggctcc acggcgtcct tccacatcgg ctgcaggatg cgctggagct cctcctggga
     2941 gtatccaaac gcggcgagtt cccgccgcag gtcgcctcca ccgagccgct tccccccgcc
     3001 tgtcccgtcc accgccggcc gggccccggt tggggccgcc cccgcctcca gacggagccg
     3061 gccggtggaa acccacgtag cgtagggacg ccgccgggcg cgctcctcct tgatcgcgtc
     3121 gttcgtgagg atgcggcccg cggccgtgtc gaccgcgatc atccgtccgg gaccgagccg
     3181 ccccttctcg acgatccgcg ccgggtcgag cgcgatgacg cccaccacgc tggccacgat
     3241 caccaggccg tcgtccatga tcgtgtaccg ggccggccgg aggccgttgc ggtcgagcgc
     3301 ggccgcggcg acccggccgt cgctgaacgc gagggccgcg gggccgtccc acggttcggc
     3361 gaggccggca tggaaccggt aaaacgcccg gaggtcctcc ggcatctcgg cgacgttctc
     3421 ccacgcctcg ggcaccagca tcatcatggc gtggagcagg tcccggccgg actgcgtcac
     3481 aagctccagg acattgtcca gcaccg
//
LOCUS       DASQGH010000044         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42660, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000044 DASQGH010000000
VERSION     DASQGH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42660"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VEW91_00750"
     CDS             <1..314
                     /locus_tag="VEW91_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYM90144.1"
                     /translation="LILAANHYIKAAVSVSGSVPSDRTASNLNAAVLSIYGENDQRDT
                     EGLTDFENAMKKAGLAFALKLEPKAGHDFFNDTSSRYVPEAAKDAWGLTLDWFAQHLK
                     G"
     gene            331..975
                     /locus_tag="VEW91_00755"
     CDS             331..975
                     /locus_tag="VEW91_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HYM90145.1"
                     /translation="MSTPTTAAEALRAILRAKVVPIIRTASRDWAARVAEVLADAGLG
                     VIEVTFTVPDAAQVIRSLRSQFPDVLVGAGTVTHGAIAEQAVDAGAQFLLSPALSPGM
                     VEVARRHGVLAVPGAFTPTEVLQALDQGAELVKIFPAEIGGPRHIRATLAPLPHARLL
                     PTGGVTPENIAEWLDAGAAAVGIGTALVGPGNRPVDMAALRARTGALMQALAAL"
     gene            962..1963
                     /locus_tag="VEW91_00760"
     CDS             962..1963
                     /locus_tag="VEW91_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HYM90146.1"
                     /translation="MPRSRAPRTSSRASRPAATVVGIGETLVRLAPQGGDTLETASTL
                     SVLIGGAESNVCATLAHLGVPTAWISRLPSTPLGRRVAATIRGYGVNIDGVLWAPEGR
                     VGLLLLQPGTGPRAGEVQYYRLDSAFAAIDPHAVAWDLLDGARVVHLTGITPALGANA
                     SRLVACAIAEARRRGVRVSFDVNYRAKLWDPRAARDGIEPFLHGLDIVILNDRDAATV
                     FGERGAPTTVVRRLRARFRCGVLVLTCGGAGAVAQDRAGTHRQPAYPTEIVDRIGRGD
                     AFAAGFLYGYLARGTAQSLRYGAALAALKQTYRGDVCFATPEAVHAVLRGEGGAFQR"
BASE COUNT          277 a          697 c          714 g          291 t
ORIGIN      
        1 ggctgattct ggcggcgaac cactacatca aagcggcggt ctcggtttcc ggaagcgtcc
       61 cgtcggaccg cacggcatca aacctgaacg cggccgtgct gtcaatctac ggggagaacg
      121 accagaggga tacggaaggg ctcaccgatt tcgagaacgc gatgaagaag gccgggctgg
      181 catttgccct gaagctcgag cccaaagccg gccacgactt cttcaacgac acctcgtccc
      241 gctacgtccc cgaggccgca aaggatgcct ggggactgac cctcgactgg ttcgcgcagc
      301 acctcaaggg atagcggccg gccggaccgc atgagcaccc cgaccaccgc cgcggaggcg
      361 ctccgggcca tcctccgggc caaagtggtg ccaatcatcc ggacggcgtc ccgtgactgg
      421 gcggcgcggg tggctgaggt gctggccgat gcggggctgg gggtcatcga ggtgaccttc
      481 acggtcccag atgccgcgca ggtcatccgc agcctccgat cgcagttccc cgacgtgctg
      541 gtcggtgccg ggaccgtcac ccacggcgcg atcgccgagc aggcggtcga cgccggcgcg
      601 cagttcctgc tgagccccgc gctctccccc ggcatggtcg aggtcgcccg ccggcacggg
      661 gtgctggccg tgccgggcgc cttcacgccc acggaggtgc tccaggcgct cgatcagggt
      721 gcggaactgg tcaagatctt tcccgccgag atcggcgggc cgcgccacat cagggcgacg
      781 ctggctccgc tcccccacgc gcggctgctg ccgacgggtg gggtcacccc cgagaacatc
      841 gccgagtggc tcgatgcggg cgcagcggcg gtcggcatcg gcaccgcgct cgtcggtccg
      901 gggaaccggc cggttgatat ggcggcgctt cgcgcgcgga ccggcgcgct gatgcaggct
      961 cttgccgcgc tctagggcgc cccgcacctc cagccgcgcg agccgtccgg cggcgacggt
     1021 cgtaggcatc ggggagacgc tcgtccggct cgctccgcag ggcggagaca cactcgaaac
     1081 cgcttcaacg ctctcggtgc ttatcggcgg cgccgagtcc aacgtttgcg ccacgctggc
     1141 tcacctcggg gtcccgaccg cctggatctc gcggctgccc tccacccccc tcggccggcg
     1201 agtcgcggcg accatccgcg ggtatggagt gaacatcgac ggggtcctgt gggctcccga
     1261 gggaagggtc ggcctgctcc tgttgcagcc gggcaccggc ccaagagccg gcgaggtaca
     1321 gtactaccgg ctcgactccg cgttcgccgc gatcgatccc cacgccgtcg cctgggatct
     1381 cctcgacggc gcgcgcgtcg tgcatctcac cggaatcacg ccggcgctcg gcgcgaatgc
     1441 gtcgcgcctc gtcgcgtgcg cgatcgccga ggcccggcgg cgaggggtcc gggtctcgtt
     1501 cgacgtcaac taccgcgcga agctctggga tccgcgcgcc gcccgggatg gcatcgaacc
     1561 atttctccat ggcctcgaca tcgtgatcct gaacgaccga gacgccgcga ccgtgttcgg
     1621 cgagcgggga gcgcccacca cggtcgtgcg gcggttgcgt gcgcgcttcc gctgcggggt
     1681 cctggtgctg acgtgcgggg gggcaggagc ggtggcccag gatcgggcag gcacccaccg
     1741 ccagccggcg tacccgaccg agatcgtcga ccggatcggg cgcggggatg cgttcgccgc
     1801 cgggttcctc tacgggtatc ttgcgcgcgg gaccgcgcag agcctgcggt acggggcagc
     1861 gctggcggcg ctgaagcaga cgtaccgggg cgatgtgtgt tttgcgacgc ccgaggccgt
     1921 gcacgccgtg ctgcgcgggg aagggggggc gttccagcgc tgagccggcc tcggctcag
//
LOCUS       DASQGH010000045         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22842, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000045 DASQGH010000000
VERSION     DASQGH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22842"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VEW91_00765"
     CDS             <1..528
                     /locus_tag="VEW91_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYM90147.1"
                     /translation="EDPEALHSGLQVIAEVTADFVRAVMAAGADGVFFSTQMATTDLL
                     TREEYEEFGRPYDLQILEAASTGLTFLHIHGVHIMFDIFADYPVHIINWHARETPPRM
                     ADARAQVSACLACGIDAWNTLAKKTPEAVAEEVRDAIGQTGGRGHIVTTGCVMPVDTP
                     EVNIRAAIAAARESG"
     gene            592..>2053
                     /locus_tag="VEW91_00770"
     CDS             592..>2053
                     /locus_tag="VEW91_00770"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HYM90148.1"
                     /translation="MGQNLTRKILKAHLADGRMAVGEEIGIRIDEILVQDITGTAAMQ
                     EFEAMQLGRVRCKVAVVFGDHNVLQVSEENTEDHLYLATAARKYGMWWAKPGAGIGHQ
                     IHQEHFACPGDTALGADSHTPHIGGMGVYAMGAGGLDVAVAMGGGPYYFDMPAVVRVH
                     LTGALRPWSTAKDVILEMLRRKTAKGGFGKVFEYAGPGIRNLNVQQRITICNMGAELG
                     ASTSIFPSDDVTRDYMRRIGREKDWREALPDPDADHDESMELDLGSIEPLVALPSNPD
                     KVVPVSQAAGVKVDQVMVGSCTNGSYMDLRAVAEIMKGRTVHPDVTFFIHPSSRLDLE
                     VLAREGLLTELIAAGVNVEAATCGACIGVGHVPAKGMKSLRAINRNFKGRTGQKEDEG
                     YLSSSEVAAASAITGVITDPRKLGISAPAQALPDRLDLTNPSLVPPASPAEAASLKVI
                     KGENITTIPLKAPLTDALRGEVLIALGDNISTDHIMP"
BASE COUNT          378 a          684 c          710 g          281 t
ORIGIN      
        1 gaggaccctg aagcgctgca cagcgggctc caggtgatcg ccgaggtgac cgccgatttc
       61 gtgcgggcgg tgatggcggc gggcgccgac ggggtgttct tctccaccca gatggccacg
      121 accgatctgc tcacgcgcga agagtacgag gagttcggcc ggccgtacga cctgcagatc
      181 ctcgaggccg cgtcgaccgg gctgacgttt ctgcacatcc acggggtgca catcatgttc
      241 gatatcttcg ccgactaccc cgtgcacatc atcaactggc acgcgcgcga gacgcctccc
      301 cggatggcgg acgcccgggc ccaggtcagc gcgtgtctgg cctgcggcat cgatgcgtgg
      361 aacacgctgg cgaagaaaac ccccgaggcg gtcgcggaag aggtgcggga cgcgatcggc
      421 cagaccgggg gacgcggcca catcgtcacg acgggctgcg tcatgccggt ggacaccccc
      481 gaggtcaaca tccgggcggc gatcgccgcc gcccgcgaga gcggctgagc gaccccgcgc
      541 ccctacggct tataatggta aggtagagcg gtccccgatg ggagaagtgt catggggcag
      601 aacctgaccc gcaagattct caaggcgcat ctcgcggacg gccggatggc cgttggtgaa
      661 gagatcggca tccgcatcga tgagatcctc gtccaggaca tcaccggcac cgcggcgatg
      721 caggagttcg aggcgatgca gctcgggcgg gtacggtgca aggtggcggt ggtgttcggc
      781 gaccacaacg tactgcaggt cagcgaggag aacaccgagg accacctgta tctcgccacc
      841 gcggcccgca agtacggcat gtggtgggcg aagccggggg cggggatcgg ccatcagatc
      901 caccaggagc acttcgcctg cccgggcgat acggcgctcg gcgccgacag ccacaccccc
      961 cacatcggcg ggatgggggt ctacgccatg ggcgccgggg gactcgacgt ggcggtggcg
     1021 atggggggcg gcccgtacta cttcgacatg cccgccgtgg tccgcgtcca cctgaccggc
     1081 gcgctccgcc cgtggtcgac ggccaaggac gtcatcctcg agatgctgcg ccggaagacc
     1141 gccaaaggcg gcttcggcaa ggtgttcgaa tacgccggac ccggcatccg aaacctgaac
     1201 gtgcagcagc ggatcaccat ctgcaacatg ggcgccgagc tgggcgcgag cacgtcgatc
     1261 ttcccgtcgg acgacgtcac ccgggactac atgcggcgga tcgggcgcga gaaggactgg
     1321 cgggaagccc tgcccgatcc cgacgccgac cacgatgaat cgatggagct cgacctcggg
     1381 agcatcgagc cgctcgtcgc gctcccgagc aaccctgata aggtggtccc cgtctcccag
     1441 gccgcggggg tcaaggtgga ccaggtgatg gtcggctcct gcaccaacgg ctcatacatg
     1501 gatctgcgcg ccgtggccga gatcatgaag ggccgcacgg tccaccccga tgtcaccttc
     1561 tttatccacc cgagcagccg cctcgacctg gaagtgctgg cccgcgaggg gctgctcacc
     1621 gaactgatcg ccgccggcgt gaacgtggaa gcggccacgt gcggggcgtg catcggggtc
     1681 gggcacgtgc cggccaaggg aatgaagagc ctgcgggcca tcaaccgcaa cttcaaaggg
     1741 cggacgggcc agaaagagga cgagggctac ctgagctcca gcgaggtggc cgccgcgtcg
     1801 gcgatcacgg gggtgatcac ggatccgcgg aaactgggga tctccgcccc cgcccaagcg
     1861 ctcccggaca ggctggacct cacgaacccg agcctggtgc cccccgcatc cccggcagaa
     1921 gccgcatcgc tcaaggtgat caagggggag aacatcacca cgatcccgct caaggcgccc
     1981 ctcaccgacg cccttcgggg cgaggtgctg atcgcgctcg gggacaacat cagcacggac
     2041 cacatcatgc ccg
//
LOCUS       DASQGH010000046         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39835, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000046 DASQGH010000000
VERSION     DASQGH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39835"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VEW91_00775"
     CDS             <1..692
                     /locus_tag="VEW91_00775"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HYM90149.1"
                     /translation="TPPLGFVISMEGADPILDPGHLGEWWEEGLRLLGLTHYGPGRYA
                     GGTGTEVGLSGLAAPLLSEMARLGMILDLTHCSDQAFWEALSHFPGRIIASLNNCRAL
                     VPHQRAFSDDQIRAIVERDGIIGVAMDCWMIAPGWRHGDSNEKIGLGDVVNHIDRICQ
                     LAGDCRHAAIGSDLDGGFGREGSPHDLDTIADLQQLASLLEARGYRRDDAAAIMHGNW
                     AALLRRAWTRG"
     gene            807..1316
                     /locus_tag="VEW91_00780"
     CDS             807..1316
                     /locus_tag="VEW91_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HYM90150.1"
                     /translation="MASLAPGASSSSPADPTVARLSGLSGDAFDIAFLQAVIPVNDEA
                     IEMAMTATLYGDHTELLRWNQTVVERKNAQIRQLLAWLQEDGARPTERKAGVATPSVK
                     KLRTLRGPALERAYLPLMAAQLDRTATLAHLASQKAHRPALRTFAKDDVRMDGQDSTT
                     LRGWMKKWY"
     gene            1445..2148
                     /locus_tag="VEW91_00785"
                     /pseudo
     CDS             1445..2148
                     /locus_tag="VEW91_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
BASE COUNT          328 a          691 c          749 g          380 t
ORIGIN      
        1 cgacgccccc gttggggttt gtgatcagca tggaaggcgc ggatcccatc ctggacccgg
       61 ggcatctcgg ggagtggtgg gaggaagggc tccggctcct cggcctcacc cactacgggc
      121 cgggaagata cgcggggggc accggcacgg aggtggggtt gagcgggctc gcggccccgt
      181 tgctgtcgga gatggcgcgt ctggggatga ttctcgacct cacccactgc tccgatcagg
      241 cgttctggga ggccctgtcg catttccccg ggcggatcat tgccagtctc aacaactgcc
      301 gcgcgctagt gccgcaccag cgggcgttct ccgacgacca gattcgcgcg atcgtcgagc
      361 gggacgggat catcggcgtc gcgatggatt gctggatgat cgcgcccggg tggaggcatg
      421 gcgacagcaa tgagaagatc ggattggggg acgtggtgaa ccacatcgac cgcatttgcc
      481 aacttgcggg ggactgccgt cacgccgcga tcggaagcga tctggacggt gggtttggcc
      541 gggagggctc cccccacgac ctcgacacca ttgcggacct ccagcagctc gcctctttat
      601 tggaagcgcg aggctatcgg cgggatgatg ccgccgccat catgcacggg aactgggcgg
      661 cgctgctgcg gagggcctgg accagagggt agctcggcca aggtctcgat caccgcgatc
      721 tggggcataa ttgtgtatcg aaccaaactc cgccagcgag gtgcacaatg cgtatcgtgc
      781 ttgcgattgg catcctggtc cttgccatgg cttcccttgc ccctggagcc tcgtccagtt
      841 cccccgccga cccgaccgtc gcccggttgt cgggcctgag cggagatgca ttcgatatcg
      901 ccttcttgca ggcggtcatt cctgtgaatg atgaagcgat cgagatggcg atgaccgcca
      961 cgttgtacgg ggatcatacc gagttgctcc gctggaacca gacggtggtg gagcgcaaga
     1021 acgcgcagat tcgccagctg ctcgcctggc tgcaggaaga tggcgcccgc cccaccgagc
     1081 gcaaggccgg cgtggcgacc ccttcggtca agaagctccg gacccttcgt ggcccggccc
     1141 tcgagcgcgc gtacctgcct ctgatggcgg cccaactcga ccgcaccgcc acgttggcgc
     1201 atctcgcctc gcaaaaggcg caccgtcccg cgctgcggac gtttgccaag gacgacgttc
     1261 ggatggacgg ccaggactcc accacgttgc gcggatggat gaagaagtgg tattgaggcc
     1321 cgcgtaagtc gcgttctgca cgtctgaccc ttgccgaccc ggcgcactgc gccgggtcgg
     1381 ctgcgctcgg gggcaggtgg gggttgtgca ggtggcgccc cggccaggcg gagaaggtag
     1441 gggcatgtcg cggagtcccg gagccggcgc cagcaaggtg ctctcgctgg agaccgcagc
     1501 ccgcctggtt cccgacggag cgctggtgac gtttgggggc gtcctgctca accgcccgcc
     1561 ggccgcgttc gtccgagaaa tggcccggca gcggcggcgg gggcttcgcc tggtcaagcc
     1621 gtctcccgcc tacgacctcg acctgctcgc tgcggctggg tgtctggccg aggccgcgat
     1681 cgggatcacg acgtttgagt cgcgcttcgg ccaaagccgg cagtttcgca gggcggtgga
     1741 gcgcgggacg ttgaaggtcc gcgagcattc ctgagcgacc attctcgcag ggatgcaggc
     1801 ggcatccctc ggcattccgt tccagccggt gcggggactc ttcggcacgg atgtggtgac
     1861 ttccagcggc ttcgccaccg ttcgcgatcc gtattccggt gaggacgtct acgtcgtccc
     1921 gcgtatccgt ccggactggg ccgtgctgca cgtccacgag gcggacgacc agggcaacgc
     1981 gcgtctctac ggttctccgg gatacgatct tgtcatggcg gaagcggccg cccgcgtcat
     2041 cctgaccacc gagcggctcg tgccgggcga ggagttcctc gcgcatcccg aacgcacggg
     2101 aattcccgcc atcttcgtcg cggcggttgt tgtcgtgccc aacggcgc
//
LOCUS       DASQGH010000047         9854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45511, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000047 DASQGH010000000
VERSION     DASQGH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45511"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..1793
                     /locus_tag="VEW91_00790"
     CDS             546..1793
                     /locus_tag="VEW91_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HYM90151.1"
                     /translation="MTQRAVAGVTATLVAVLLVAGAPAQGAVFSDIQGDPNQRAIEKL
                     TITGVLGGFPDGTFKPGQPVTRLAAVAALAKGLDVKGAGPIPAYKDLAEIPEEVRPAI
                     SALLNTGAVSQQRAEVKKGDLLYTLATDKAVYGTEDPVDLTMTVTNTGKDDAKFVFPT
                     TQQFDFVIKRGEAEIAKWSLGQTFVQGGLDLILAVGKSFVYNTRWLQKDQDNHFVPAG
                     PYEIIGISPAKDNVVTVSLQFQKGLLSSFPDNTFRPNGQVSRAEFAALLVRALGRQGE
                     ATQKASAPLSIKDAADVPATLHGYVAVAIEQHIMAPSADGSFRPNAPTTRGEAAGALA
                     FVMNSLNRFNYVKGKLVSITQNNITVTIEAQAVQSYALTPQAAVYRNDKAVAPADLKP
                     NDQVLMLLTGPRGRAGYIEATGQ"
     gene            1869..3128
                     /gene="rho"
                     /locus_tag="VEW91_00795"
     CDS             1869..3128
                     /gene="rho"
                     /locus_tag="VEW91_00795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYM90152.1"
                     /translation="MAIAELATKSLDELQDLAKDLSIPNFRRFRKQELIMKILQVQTE
                     QSGLLFRSGILEIMQEGYGFLRTSGYLPGSEDIYVSPSQIKRFGLRVGDEVLGQVRAP
                     KDNEKYYALLRVEAVNGLDPEQARSRPDFEKLTPVFPHERLKLELPQSDLTVRIVDLF
                     APIGKGQRAMIVSPPKAGKTTLLKKIGQSVVQNHQEIYLMVLLLDERPEEVTDMRRSV
                     EAEVIASTFDRPPEEHVQVADLVLERAKRLVEGNRDVVVLLDSLTRFSRANNLVIPPS
                     GRTLSGGLDPAALHRPKRFFGAARKIEEGGSITIIATALIDTGSRMDDVIYEEFKGTG
                     NMELHLNRKLQERRTFPAIDIKLSGTRREELLLNEEELRKVWVLRKSLETLDTVAMTE
                     LILDRLRKTPNNQAFLRSIIKNSENDA"
     gene            3405..4586
                     /locus_tag="VEW91_00800"
     CDS             3405..4586
                     /locus_tag="VEW91_00800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYM90153.1"
                     /translation="MAERTRLAAAGLFLALALAVVPMLIPLNFAGADPGHPSLDVASI
                     TPPVQVRDQDVTPRPAPSEPPLNPIASQAAAPAHMVAPGESLWKISRDTGIGVEALAT
                     ANHLTIDAVLYPGQVLAVPPVDAAPSKDALAATPAAPREGAPGETLTHKVAPGETLWG
                     IARAAGLHVETLAAVNHISLDDLLYPGQALVVPTQDGPLPPSAGTRRLRVRSVRGPVG
                     PRGAGSPALYEAVRADFGSLLRPSDGRITSRFGWRIHPIFGTREFHTGVDIANRHGTP
                     IRAAEDGIVRFAGWMGGYGRLVILVHRNGLETSYSHLSALLVTLGQRVVKGQIVGRMG
                     STGWSTGPHLLFEVRLNGVPLDPTPFLQDPASAITPAPAPQEHPMIDGSPSGLNTAPV
                     P"
     gene            4720..4923
                     /gene="rpmE"
                     /locus_tag="VEW91_00805"
     CDS             4720..4923
                     /gene="rpmE"
                     /locus_tag="VEW91_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HYM90154.1"
                     /translation="MKKGIHPNYKQTTVTCACGETFVTGSTRENIRVEICSKCHPFYT
                     GQRKFVDSEGRVQKFAKKYGIQL"
     gene            5022..6089
                     /gene="prfA"
                     /locus_tag="VEW91_00810"
     CDS             5022..6089
                     /gene="prfA"
                     /locus_tag="VEW91_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYM90155.1"
                     /translation="MANRLAALEARHDELTRALADPAVLGDPTRYQRLAREHAGLTEL
                     VTALQEYRKIAGELEGAEALAREADPEIRQMASAEKAALTDRQTRLERKIEQMLAPKD
                     PRDERNVIVELRAGAGGGEAALFAGDLLRMYVRYAERHGWRSEVLSSTPTGLGGFKEA
                     ILAIQGRGAYSRLKYESGVHRVQRVPATEASGRIHTSTATVAVLPEAEEVDIVVRPDE
                     VKIDTYRAGSAGGQNVNKVETAVRITHLPTGIVAACQDERSQHQNREKAMRILRAHLL
                     ARAVESQQAEIAQARRRQVGTGERSEKIRTYNFPQDRVTDHRIGKAVHNLPTVLDGDL
                     DDLIEALTAVDHTESLTTAGL"
     gene            6086..6985
                     /gene="prmC"
                     /locus_tag="VEW91_00815"
     CDS             6086..6985
                     /gene="prmC"
                     /locus_tag="VEW91_00815"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYM90156.1"
                     /translation="MTRVAGAPTRSRREAWAWGAGRLREAGLEGTDAALEAEVLLRHA
                     AGLTREELLARPTVGMGTAPAAVYADLITRRSLGAPVAYLVGHREFFGIDLVVDHRVM
                     IPRPETERLVEVLVGALRDHPAPVVVDVGTGSGAVAIAVARMLPRARVLATDVSPGAL
                     VVAQINAARCGVARRIAWAAGAGLEPLACLGVDRPADAVVSNPPYIPTSEIARLPREV
                     REHEPAVALDGGRDGLCVLREIIAGAARYLAPGGVLALEVAAMQDQAATVAALIAAAG
                     GFGPPGIVRDYAGAERVVVASRP"
     gene            6985..7626
                     /locus_tag="VEW91_00820"
     CDS             6985..7626
                     /locus_tag="VEW91_00820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYM90157.1"
                     /translation="MDVIGVDPASEEVPRRAVRVLGEGGLVAFPTDTFYALGANARDE
                     TAVARVFAAKRRPREAPLPVLIADREQWRALVADLPEVAMRLAARFWPGALTIVCRCG
                     PGIPPVLVGGGNAIGVRQPALPVAIGLCRAFGRPIVGTSANVHGSPAPTTAVQVALDL
                     GEAVDLILDGGRCPLARPSTVIDVTRTPPVVVRVGAVPLEAVREVLGEVAVAR"
     gene            7687..8943
                     /gene="glyA"
                     /locus_tag="VEW91_00825"
     CDS             7687..8943
                     /gene="glyA"
                     /locus_tag="VEW91_00825"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYM90158.1"
                     /translation="MDELRRTDPEIAQAILQDTDRQRYHINLIASENYASRAILEALG
                     TAMTNKYAEGYPGKRYYGGCEFVDVAERLAIDRAKALFGAEHANVQPHAGAQANMAAY
                     FAFLKPGDPMLAMNLAHGGHLTHGSPVNFSGQLFRVTPYGVDERTEQIDYEVLARLAR
                     EHRPRIIVSGATAYPRTFDFPRLRAICDDVGAIMMVDMAHFAGLVAGRVHPDPVPLAD
                     VVTSTTHKTLRGPRGGMILCKARHAPQIDKAVFPGIQGGPLEHCIAAKAVCFREASHP
                     AFRTYAARIVENARAMAEEFLRRGYHVVSGGTDNHLVLLDVRTRALTGRKAEKALDAA
                     HIIVNKNMIPFDPEKPMTTSGIRIGTPAMTTRGMGPDELRKIAGWIDEVLLAPEDPPT
                     AERVRRQTRELCVSFPIYPEPVEATR"
     gene            8944..9573
                     /gene="upp"
                     /locus_tag="VEW91_00830"
     CDS             8944..9573
                     /gene="upp"
                     /locus_tag="VEW91_00830"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HYM90159.1"
                     /translation="MGQVRVFDHPLIQHKLAILRDRRTGHKEFRELVEEIAMLMAFEA
                     TSDLPTSPIEIATPLATARGVTIAGQEIAVIPILRAGLAMEAGILRLMPTARVGHIGI
                     FRDPASLEPHTYYSKLPLDIADRQVLVVDPMLATGGSAVESIDLLKQRGARIIRLMVL
                     VAAPEGIRRVQAAHPDVDIYTAAVDERLNDHGYILPGLGDAGDRLYGTR"
     gene            9594..>9854
                     /locus_tag="VEW91_00835"
     CDS             9594..>9854
                     /locus_tag="VEW91_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HYM90160.1"
                     /translation="MARPTWDEYFMSLARLAATRSTCLRRQVGAVIVKDRMLLSTGYN
                     DTPRGLPNCGDGGCARCRSGAPAGSGLDTCLCLHAEQNAIIQA"
BASE COUNT         1653 a         3282 c         3438 g         1481 t
ORIGIN      
        1 tggtgctggt cgatgccgcg cggagggccc tgcggaatac gttggagctc gccggggtgg
       61 cggcggcgga gcggatgtag cctgggcgcg tgctgttgcc gcctgcggcg tggtagtgta
      121 caatggagtc gaacgttgtt gtcttgcccc ccgttggatt accatgctgc gctcgacggc
      181 tcgtccctcc gcaatcttgc gggaggtcga ggagccatga ggggatggga aggagaatcg
      241 gacccagcgg cgaatacgcg gcacgaaagt ttcgtgggtg gccccgccgt acgcgcgaag
      301 ccggtcgcac catgcgtggg tcctgtgggt ggtgtcactg atccagcgga cgtgcccccc
      361 gagcggccgg ctcccgcgca gtctcccctc ccgcccgtga tcccttctcc tgtcagttca
      421 cccacaggcc ggttcgcgac ggggtgcctg cggggtcccg cgcaccaccc gatgcgacgg
      481 ggctgccagg acgcctgtga accctgacaa tggggcatgt acctatgtgg ccaaggaggt
      541 ctggaatgac gcaacgcgct gtggcggggg tgacggccac cctggtggcg gtactgttgg
      601 tggccggcgc accagcccaa ggagcggtat tctcggacat ccagggtgat cccaaccagc
      661 gagcaatcga gaagctcacg atcacgggtg tgctcggcgg gttccccgac gggacgttca
      721 agcccggcca gccggtcaca cggctcgcgg cggtggcggc cctcgcgaag gggttggacg
      781 tcaaaggggc agggccgatc ccggcctaca aggatctcgc ggagatccct gaggaggtgc
      841 ggcccgccat ctcggccctg ctgaacaccg gcgcggtttc gcagcagagg gccgaggtga
      901 aaaagggcga cctcctgtac accctggcga ccgacaaggc ggtgtacggc accgaggatc
      961 ccgtggacct gaccatgacg gtgacgaaca caggaaagga cgatgccaag ttcgtgttcc
     1021 ccacgaccca gcagttcgac ttcgtcatca agcgaggcga agcggagatc gccaagtggt
     1081 cgctcgggca gacgttcgtc cagggcgggc tcgatctcat cctggcggtg gggaagtcct
     1141 ttgtgtacaa cacgcgctgg ctccagaagg accaggacaa ccattttgtg ccggccggtc
     1201 cctacgagat catcggcatc tcgcccgcca aggacaacgt ggtgacggtg agcctgcagt
     1261 ttcagaaggg cctcttgtcc tcgtttcccg ataacacctt ccgccccaac gggcaggtaa
     1321 gccgcgccga gttcgcggcc ctgctggtgc gggccctcgg ccgccagggg gaggcgaccc
     1381 agaaggcctc cgcgccgctc tcgatcaagg atgctgccga cgtccctgcg acgttgcacg
     1441 ggtacgtggc cgtcgcgatc gaacagcaca tcatggcgcc ctcggcggac ggctcgttcc
     1501 ggccgaacgc accgacgacc cgcggcgaag ccgccggcgc gctcgcgttc gtcatgaact
     1561 cgctcaacag gttcaactat gtcaaaggca agctcgtgag catcacccag aacaacatca
     1621 ccgtgaccat tgaggcccag gcggtgcaaa gctacgccct cacgccccag gccgccgtgt
     1681 atcgcaacga taaggcggtt gcgccggcgg acctgaaacc caacgatcag gtcctgatgc
     1741 tgctcaccgg cccccggggg cgggccggct acatcgaagc cacggggcag taaccggggg
     1801 acagagcgtc cgcaaccggg ggctccgcgc ccgagcgcgg gggaccgact gcgtggaggt
     1861 ggcacggcgt ggcgatcgcg gaacttgcaa cgaagagcct cgacgaactt caggatctgg
     1921 ccaaagatct cagcattcca aattttcgcc gctttcgcaa acaggagctg atcatgaaga
     1981 tcctacaggt gcagaccgag caaagcgggc tcttgttccg ctcgggcatc ctcgagatca
     2041 tgcaggaagg gtatgggttc ctgcgcacca gcgggtacct gccggggtcc gaggatatct
     2101 acgtctcgcc ctcccagatc aagcggttcg gcctccgggt cggggacgag gtgctcggtc
     2161 aggtgcgcgc ccccaaggac aatgagaagt actacgcgct gctccgagtc gaggcagtga
     2221 acggcctgga tccggagcag gcgcggtcca ggcccgactt cgagaaactg accccggtct
     2281 ttccacacga gcgcctcaag ctggaactgc cgcagagcga tctcaccgtc cggatcgtgg
     2341 acctgttcgc cccgatcggg aagggccagc gcgccatgat cgtctccccg ccgaaagccg
     2401 ggaagaccac gctcctcaag aagatcggcc agagcgtcgt gcagaaccac caggaaatct
     2461 acctgatggt gctcctcctc gatgagcgcc ccgaggaggt gaccgacatg cgccgctcgg
     2521 tcgaagccga ggtgatcgcc tccaccttcg accgcccgcc ggaagagcac gtgcaggtcg
     2581 ccgacctggt cctggagcgc gccaaacggc tcgtcgaagg caaccgtgac gtggtcgtcc
     2641 ttctcgacag cctgacccgg ttctcccgcg cgaacaacct ggtgattccc ccaagcggcc
     2701 ggacgctctc cgggggcctg gaccccgcgg cgctgcaccg ccccaagcgg tttttcgggg
     2761 cggcccgaaa gatcgaggag gggggcagca tcacgatcat cgcgaccgcg ctgatcgaca
     2821 cgggcagccg gatggacgac gtgatctacg aagagttcaa gggcacgggc aacatggagc
     2881 tgcacctcaa tcgcaaactg caggagcgcc ggacgttccc ggcaatcgac atcaaactgt
     2941 ccgggaccag gcgcgaagaa ctgctgctga acgaggagga gctccggaag gtctgggtgc
     3001 tccggaagag cctggagacc ctggacacgg tggcgatgac cgagttgatc ctcgaccggc
     3061 tgcggaagac gcccaacaac caggcgttcc tccgatcgat catcaagaac agcgagaacg
     3121 acgcctgagg cgcgcggcct ggcgggaacc ctccggcagg tctccccaga gttaccacca
     3181 aaccagaagc gaagggccaa gaaagtagcg gaaatccgcc ggcaaaccgg gatcgaaatc
     3241 cgcaagacac gcttcctcat ccgaacgaat tactcgaact agttcgattt gcgtctagcg
     3301 cctccggcca gagggtattt ttcggtctaa agggatctca gtccaagatc ctattcagcc
     3361 agaatacaca ccaaccaccc caataatggt ctggcggagg ccccatggcc gagcggacgc
     3421 ggcttgcggc agcagggttg tttttggccc tagcccttgc ggttgtgccg atgctgattc
     3481 ccctgaactt tgccggcgcg gatcccgggc atccctcgtt ggatgtcgca tcgatcacgc
     3541 ccccggtgca ggtccgggac caggacgtaa cgccccgccc ggcgccttcc gagccgccgc
     3601 tcaacccgat tgcatcccag gccgcggccc ccgcgcacat ggtcgccccc ggggaatcgc
     3661 tctggaagat ctcccgagat acgggcatcg gggttgaggc gctggccacg gccaaccacc
     3721 tgaccatcga cgcggtcctg taccccggcc aggtgctcgc agtccctccg gtcgacgcgg
     3781 caccctcgaa ggacgcgctc gcggcgaccc ccgcggcgcc ccgtgagggt gccccgggtg
     3841 aaacgctgac ccacaaggtc gccccgggcg aaacgctctg gggaatcgcg cgcgccgcgg
     3901 gcctgcacgt tgagacgctg gcggcggtca accacatctc gttggacgac ctgctgtatc
     3961 cgggacaggc cctggtcgtg ccgacgcagg acggtcctct cccgccgtct gcgggtacgc
     4021 gtcggctgcg tgtccggagc gtccgcggtc cggtcgggcc aaggggcgcc ggctcccccg
     4081 cgctgtacga ggcggtgcgg gccgatttcg ggtcgctgct gcggccctcg gacgggcgga
     4141 tcacctctcg gtttgggtgg cggatccatc ccattttcgg cacacgggag tttcacaccg
     4201 gggtcgacat tgcgaatcgc cacgggaccc cgatcagggc cgccgaagac gggatcgtgc
     4261 ggttcgcggg atggatgggg gggtacggcc ggctcgtcat cctggtccac cggaatgggc
     4321 tggaaaccag ctactcccac ctctcggcgt tgctggtcac cctgggccag cgggtggtca
     4381 agggccagat cgtcggccgg atgggaagca ccgggtggag cacgggcccc catctcttgt
     4441 tcgaggtgcg gctcaatggc gtcccgctcg atcccacccc cttcctgcaa gacccggcgt
     4501 cggccatcac acccgccccg gctcctcaag agcaccccat gatcgatggg tccccgtccg
     4561 gtctgaacac cgccccggtt ccgtagatcc ggttccagag actcggcagc gtccgccggt
     4621 ttgcggggga tgaccggctg tggtataatc gcgcctgttg caccgacggt cctgcgccaa
     4681 cggctcgccg ccgcgtgggg tcgaggagag gcttccacga tgaaaaaggg catccatcca
     4741 aactacaaac agacgacggt gacgtgcgcc tgcggggaga cgtttgtaac cggctctacc
     4801 cgggagaaca tccgggtcga gatctgctcg aagtgccatc ccttctacac cggccagcgc
     4861 aagttcgtcg attccgaggg ccgcgtccag aagttcgcca agaagtacgg catccagctc
     4921 tagagccccc tccacacccg gcccgcttcc cccgaggagc cgccccaggt attggtataa
     4981 tgaatgaagg aaatcacgag gaggcacgtg tgcaccctgc tgtggcaaac aggcttgcag
     5041 cgctcgaggc ccgtcacgac gagctcaccc gcgcgctggc ggaccccgcg gtgttgggcg
     5101 atccgacccg gtaccagcgg ttggcccgcg agcatgccgg cttgacggag cttgtgacgg
     5161 ccctccagga gtaccggaag atcgccggcg agcttgaagg ggcggaggcg ctggcgcgcg
     5221 aggccgatcc cgagatccgc cagatggcgt cggcggagaa ggctgcgctg acggaccggc
     5281 agacccggct cgagcgtaag atcgagcaga tgctggcccc gaaggacccg cgggacgagc
     5341 ggaacgtgat cgtggagctc cgggccgggg ccgggggtgg ggaagcggcg ctgttcgccg
     5401 gggaccttct tcggatgtac gtccggtacg cggagcgtca cgggtggaga tcggaggtgc
     5461 tgtccagcac ccccaccggt ctcggcggat tcaaggaggc gatcctcgcg atccaggggc
     5521 gcggggcgta cagccggttg aagtacgaga gcggtgtcca ccgcgtccag cgtgtgcccg
     5581 ccaccgaggc gagcgggcgg atccacacgt ctaccgcgac ggtggccgtc ctgccggagg
     5641 cggaggaggt cgacatcgtg gtccggccgg acgaggtcaa gatcgacacc taccgggccg
     5701 gaagcgcggg cgggcaaaac gtcaacaagg tggagacggc ggtccggatc acccacctgc
     5761 cgacggggat cgtcgccgcc tgccaggatg agcggtcgca gcaccagaac cgcgagaagg
     5821 cgatgcggat tctacgcgcg cacctgctgg cgcgcgcggt ggagtcgcag caggccgaga
     5881 tcgctcaggc gcggcgccgg caggttggca ccggtgagcg cagcgagaag atccggacgt
     5941 acaatttccc ccaggaccgg gtgaccgacc accggatcgg caaggccgtg cacaatctcc
     6001 ccaccgtgct ggacggcgac ctggatgacc tgatcgaggc cctcacggcg gtcgaccaca
     6061 cggagagcct cacgaccgcc gggctgtgac gcgcgtcgcg ggggccccca cccggagccg
     6121 gcgggaagcg tgggcctggg gcgcgggacg gctgcgggag gccggccttg aggggacgga
     6181 cgctgcgctg gaggcggagg tccttcttcg ccacgctgcg gggctaacgc gcgaggagct
     6241 cctggcgagg cccacggtgg gtatggggac ggcgcccgca gcagtctacg ccgatctgat
     6301 cacccgccgc tccctgggcg caccggtggc ctacctggtg ggacaccggg agttctttgg
     6361 gatcgacctg gtcgtggatc accgcgtcat gattccccgc cccgagacgg agcggctggt
     6421 ggaagtgctg gtgggcgccc tccgggacca tcccgctccc gtcgtcgtcg acgtcggcac
     6481 cggaagcgga gcggtcgcca tcgcggtcgc ccgcatgctc ccccgcgcgc gggtgctggc
     6541 caccgacgtg tcgcctggcg cgctggtggt ggcgcaaatc aacgccgcgc ggtgcggggt
     6601 ggctcgccgt atcgcgtggg ccgcgggggc gggcctcgag ccgctggcgt gcctgggggt
     6661 ggatcgaccc gccgacgcgg tggtctcgaa cccgccgtac attcccacgt cggagatcgc
     6721 ccgccttccg cgggaggttc gcgagcacga gcctgcggtc gccctggacg gagggcgcga
     6781 cggtctctgc gtgcttcggg agatcatcgc gggtgccgcg cggtacctgg cccctggcgg
     6841 cgtactggcg ctcgaggtgg cagcgatgca ggatcaggcg gccaccgtgg cggcgctgat
     6901 cgccgccgca ggcgggttcg gcccgcccgg gatcgtgcgc gactacgcgg gggccgagcg
     6961 ggtcgtggtt gcatcccgcc cctaatggac gtcatcgggg tcgaccccgc gagcgaggag
     7021 gttccgcgac gggcggtccg ggtgttgggc gagggagggc tggttgcgtt tcccaccgat
     7081 accttctacg cccttggcgc gaacgctcgg gatgagaccg cggtggcacg ggtgttcgcc
     7141 gccaagcggc gcccccgcga ggccccgctg cccgtgctga tcgccgaccg ggagcagtgg
     7201 cgggcgctgg tggcggatct cccggaggtc gcgatgcggc tggcggcccg gttctggccc
     7261 ggcgccctga cgatcgtgtg ccggtgcggg ccgggcatcc ctccggtgct ggtgggcggc
     7321 ggcaatgcga tcggggtccg ccagccggcg ctgccggtgg cgatcggact ctgccgcgcg
     7381 ttcggaaggc ccatcgtcgg cacgagcgcg aatgtgcatg gaagccccgc ccccactacg
     7441 gcggtccagg tcgcgctcga cctcggcgag gcggtggacc tgatcctcga cggagggcgg
     7501 tgccccctcg cgcgtccctc gacggtgatt gacgtgacgc gcacgccccc cgttgttgtg
     7561 cgggtggggg cggtcccgct cgaggcggtg cgcgaggtgc tcggcgaggt ggcggtggcc
     7621 cgctagggca gggttccccc cgcgggcggg gaagggagag agcggacgca gaaggaggcg
     7681 agcactgtgg acgagcttcg ccggacggat cccgagatcg cgcaggccat cctgcaggac
     7741 accgatcgcc aacgctacca catcaacctg atcgcatcgg agaactacgc cagccgggcg
     7801 atcctcgaag cccttggcac cgcgatgacc aacaagtacg cggagggcta tccgggcaag
     7861 cgctactacg ggggttgtga gtttgtggac gtggccgaac ggctggcgat cgatcgcgcc
     7921 aaggccctgt tcggcgccga gcacgcgaac gtccagcccc atgcaggggc gcaggccaac
     7981 atggccgcgt acttcgcctt cctcaagccg ggcgacccca tgctggcgat gaacctggcg
     8041 cacgggggac atctgaccca cggcagcccg gtcaacttct ccggccaact cttccgcgtc
     8101 accccgtacg gggtcgacga gcggaccgag cagatcgact acgaggtcct ggccagattg
     8161 gcccgcgagc atcggccgcg gatcattgtc tcgggggcga cggcgtaccc ccggaccttc
     8221 gatttccccc ggctcagggc gatctgcgat gacgtcgggg cgatcatgat ggtggacatg
     8281 gcgcacttcg cggggctcgt ggcaggccgg gtccacccgg acccggtccc gctcgccgat
     8341 gttgtcacgt ccacgaccca caagacgctt agaggcccgc gcggcgggat gatcctctgc
     8401 aaggcccggc acgcccccca gatcgacaag gcggtgttcc cgggcatcca gggcggaccg
     8461 ctcgagcact gcatcgccgc caaggcggtg tgcttccgcg aggcctcgca tccggcgttt
     8521 cggacctatg ccgcccgcat cgtcgagaac gccagggcga tggccgagga gttcctccgc
     8581 cggggctatc acgtcgtgtc cggcggcacg gacaaccacc tcgtgctcct cgatgttcgg
     8641 acgagggcgc tgacggggcg gaaggccgag aaggcgctcg acgccgccca catcatcgtc
     8701 aacaagaaca tgatcccgtt cgacccggag aaaccgatga ccaccagcgg gatccggatc
     8761 ggcacgccgg cgatgaccac ccgggggatg ggccccgacg aactccggaa gattgccggc
     8821 tggatcgacg aggtcctcct cgctccggaa gaccccccga cggccgagcg cgtgcggcga
     8881 caaactcgag agttgtgcgt gtcgttcccg atctatcccg agccggtgga ggcgacgagg
     8941 taggtgggcc aggtccgggt cttcgaccac cccctgatcc agcacaagct cgcgatcttg
     9001 agggaccggc gaacggggca caaggagttc cgcgaactgg tcgaggagat cgccatgctg
     9061 atggcgttcg aggccaccag cgatcttccc accagcccga tcgagatcgc caccccgctc
     9121 gccacggcgc gcggggtgac gattgccggc caggagatcg ccgtcatccc gatcctccgg
     9181 gccggccttg cgatggaggc ggggatcctc cggctgatgc cgaccgcgcg ggtggggcac
     9241 atcgggatct tccgcgatcc ggcgtcattg gagccgcaca cctactacag caagctgccc
     9301 ctcgacatcg ccgaccgtca ggtgctggtg gtggacccca tgctggcgac cgggggatcg
     9361 gccgtcgaat cgatcgatct gctgaagcag cggggggccc ggatcatccg gctgatggtc
     9421 ctggtcgcgg cccccgaggg catccggcgc gtccaggcag cgcaccccga tgtcgacatt
     9481 tacaccgcgg cggtagatga gcggctgaac gaccacgggt atatcctgcc ggggctgggc
     9541 gacgccgggg accggctgta cgggacccgt tagaggattc cggggaggat cgcatggctc
     9601 gaccgacgtg ggacgagtac ttcatgagcc tggcacgcct ggccgcgacc cggtcgacct
     9661 gtctgcgccg ccaggtcggc gcggtcatcg tcaaggaccg gatgcttctc agcaccgggt
     9721 acaacgatac cccgcgcggc ctcccgaact gcggggacgg cgggtgcgcg cggtgccggt
     9781 cgggcgcgcc ggccggatcc gggctcgata cctgcctatg cctccacgcc gagcagaacg
     9841 ccatcatcca ggcc
//
LOCUS       DASQGH010000048         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39838, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000048 DASQGH010000000
VERSION     DASQGH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39838"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..860)
                     /gene="prfB"
                     /locus_tag="VEW91_00840"
                     /pseudo
     CDS             complement(1..860)
                     /gene="prfB"
                     /locus_tag="VEW91_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(1067..3187)
                     /gene="lnt"
                     /locus_tag="VEW91_00845"
     CDS             complement(1067..3187)
                     /gene="lnt"
                     /locus_tag="VEW91_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYM90161.1"
                     /translation="MPRAQRIAPSSRIGRPPPGAGGPRVLGAESLEFAKPDRPVRVAH
                     ILAVVVSGLGFVLAFPQPDLGFLGWITLVPLLLATHHHAPRAAFWLGALWGLVAYGGV
                     FWWSTTFGVAAWALIVVLGAVFPGLAMLAVAWVEHDRDSPWIFLWIASVWTAVEFLRS
                     QGALGFPWALLGASQHQSVTVVQIASVTGIYGVSFLVALVNAALYVILTRRAVVIPVC
                     GTGIAVAGALLWGALVLRHPVPSTLVAAAIQPNFAVRAGDGPEIVQRALADLERLTHD
                     AAGRGARLVVWPETASPADILSPPVLAAVRSWARADRVSVIATSLEGGRTNSAFAFAP
                     SGMLVGRYDKVRLVPFAEAGEHPGHPPAVLRTPQGQLGMAICFESIFPELARQAVRDG
                     ADLLAVITNDAWFDGRSAAAQHAALAPFRAVEEGRYLIRAANQGISAIIDPRGRTLDE
                     LPLGRRGILTARVAPLHGLTVYARIGDAFSWIVVLVAVGLLLPRALAFVAEEAGTPAF
                     SRLITHSALPLLAVGGTAWVQGPGAVASGPLGVPLPLVALLCVTALLSLGHPLRGLGF
                     RTGGFVPALALGLAGVGGLALVVRHAFASHGTAPEIPLPGGGGWSGTAVQALAAGLAL
                     EWWLRGLAFADATAWRGWRVAVVWSALLGMAAASWRGAEAMVWALYMGAVLGLVRARW
                     AQVPALAVAHGAGNVLLGFLTSLW"
     gene            complement(3166..>4181)
                     /locus_tag="VEW91_00850"
     CDS             complement(3166..>4181)
                     /locus_tag="VEW91_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HYM90162.1"
                     /translation="GFVPLPPGLSEDSLRKLDATGVKEQIRTAALAAYEAKVASVGPE
                     TMREVERLIGWIVEDNVPLEHKMLSRQIETAQKKIESYHFDVRKHVLEYDDVMNVQRQ
                     VIYAERRKILEGSGVRENVLNFIESLVGDLVEGFCSAEARPEEWDLAGLLEEIRGFVP
                     LPPGLSEDSLRKLDAAGVKEQIRTAALAAYEAKVASVGPETMREVERLILLQTLDRKW
                     IDHLYAMDALREGIGLRAYGQANPLTEYQKEGYALFEQLKHSVQEETVKFLYLVQVQP
                     AAAVPVQPARPAYRVTRQGGDGDRPRSPVGGAAPRAAKIGRNDPCPCGSGKKYKKCHG
                     RNE"
BASE COUNT          689 a         1510 c         1404 g          578 t
ORIGIN      
        1 taggggtgaa gaacgtagga gcgaatctgg ttcccccagg cggcgtcccg gtgctccccc
       61 cgcagggcgg tcagcttctg ctgctgttcc tcgtgctgca actcgtagag cctcgcctgg
      121 agcagcttca tcgcggtcag cttgttggca tgctgcgagc gttcgttctg gcactggacg
      181 acgatcccgg tcggtacgtg ggtgatccgc accgccgtct ccaccttgtt gacgttctgg
      241 ccgcccgcgc ccccggaccg ataggtatcg atccggagct cgtcctcctt gacggccact
      301 tccgcggcct ccacctcggg gatcacatcg acgagcgcaa aggacgtatg acggcggtgg
      361 gccgcgtcgt acggcgagag gcgcaccagg cggtgcacac cccgttcccc acggaggtag
      421 ccgtaggcgt tcgtcccgga cacgaacacg gtaacgctct tcatccccgc ttcctcgccg
      481 ggagagagat ccacgacgtc ggtgcgatag ccatgggctt ccgcccaccg caggtacatc
      541 cgcaggagca tctcgaccca gtcctgggac tcggtaccac cggcgccggc gtgaatcgaa
      601 agaatggcgg tgcgggcatc gtgctcgccg ctgaacaggg tggtcagttc gaggcggtcc
      661 atcgtgctgc cgagcgcccg aacatcgcgc tcgacgtcgg cgagggcgct tgtgtcctcg
      721 cgcgcagcga gatcaatgaa ttcccgtaga tcggccacct cccgctcgag gtggctcagc
      781 tcatcgacct gtgcgcgcag cgccgcgacc tctcgggacg tccggcgggc tgcctccgga
      841 tccgcccaaa aagtcggcgc ctgcatttgg cgttcaagct cggtgagacg ggcctgctgg
      901 cggggaaggt caaagatgcc tcccgatgct ccgcacgcgc ccctggtact cgtcgagggt
      961 acgtcgcagt tcttcgatcg tcacgcctac cgccctccca tcgtcgcccg ccggcggacg
     1021 cgccggatct gcggcccgcc gcgcggctaa cgccctatta tagcactcac cacagcgaag
     1081 tcaaaaagcc gaggaggacg tttccggccc cgtgggcgac cgccagggcg ggcacctggg
     1141 cccatcgcgc gcgaacgagg cccagcaccg cacccatgta cagcgcccac accatcgctt
     1201 cggcgccgcg ccatgacgcg gcggccatgc cgagcaacgc cgaccagacc accgccaccc
     1261 tccagcctcg ccacgcggtg gcgtcggcga aggcgaggcc gcgcagccac cactccaggg
     1321 ccaggccggc cgcgagcgcc tgcacggcgg tgccgctcca ccctcccccg cccgggagcg
     1381 ggatctcggg cgcggtcccg tggctcgcga acgcgtggcg cacaacgagc gcgagtccac
     1441 cgaccccggc cagcccgagc gcgagcgcgg gcacaaagcc cccggtccga aacccgagcc
     1501 cccgaagagg gtgtcccagg ctcaggaggg cggtcacgca gagcagcgcc acgagcggga
     1561 gcggcacacc cagggggccc gaagcgacag cgccgggccc ctgcacccac gcggtcccgc
     1621 cgaccgccaa aagcggaagc gccgagtggg tgatgaggcg ggaaaacgcc ggggtcccgg
     1681 cctcctcggc gacgaaggca agcgcgcgcg gcagcagcag gcccacggcg acgagcacga
     1741 cgatccagct aaaggcatcg ccgattcggg cgtacaccgt caggccgtgg agcggcgcca
     1801 cgcgggcggt gaggattccc cgccggccca ggggaagttc gtcgagcgtg cgaccccgcg
     1861 gatcgatgat cgcgctgatc ccctggttgg cggcgcgaat gagataccgc ccttcctcga
     1921 cggcgcggaa cggcgccagg gccgcgtgtt gggccgccgc ggaacggccg tcaaaccagg
     1981 catcgttcgt gatgacggcg agtaggtccg ccccgtcccg caccgcctgc cgggcgagtt
     2041 cgggaaagat gctttcaaag cagatcgcca tgccgagctg cccctgcggc gttcgcagga
     2101 ccgccggggg gtgccccgga tgctcgccgg cctcggcaaa cggcacgagc cgcaccttgt
     2161 cgtaccgccc caccagcatc ccggagggcg caaacgcgaa cgccgagttg gtccgtcccc
     2221 cttcgaggct cgtggcgatc acggagacgc gatcggcgcg cgcccacgac cggaccgctg
     2281 cgagcacggg gggactgagg atgtccgcgg gggaggcggt ctccggccag acgaccagcc
     2341 tcgcgccgcg gccggcggcg tcgtgggtca accgttccag gtcggcgagg gcgcgctgaa
     2401 cgatctcggg gccatccccc gcccggacgg cgaagttggg ctggatggcg gcggccacca
     2461 aggtggacgg aacggggtgg cgaagcacga gggcgcccca caagagcgcg ccggccaccg
     2521 cgatcccggt tccgcacacc gggatgacga ccgcgcggcg ggtgaggatc acgtagaggg
     2581 ccgcgttgac gagcgcgacg aggaaactca ccccatagat tccggtcacc gaggcgatct
     2641 gcacgaccgt gacggattgg tgctgggacg cccccagcag cgcccacgga aacccgagcg
     2701 cgccctggct gcggagaaac tccacagcgg tccacaccga ggcaatccag aggaagatcc
     2761 atgggctgtc ccggtcgtgc tcgacccatg cgaccgcaag catcgcgagg cctgggaaga
     2821 ccgcgccgag gacgacgatc agcgcccagg cggccacccc aaacgtcgta ctccaccaga
     2881 acacgccacc gtaggcgacg agcccccaga gggccccgag ccagaacgcg gcgcggggcg
     2941 cgtggtggtg cgtggcgagc aggagcggga cgagcgtgat ccacccgagg aaccccaagt
     3001 ccggctgcgg aaaggcgagg acgaagccaa ggccggagac caccaccgcc aggatgtggg
     3061 cgacgcgaac agggcggtcg ggcttcgcga actccaggga ctcggctccg agaacacgcg
     3121 gcccccctgc ccccgggggc ggccggccga tccggctgga cggcgctatt cgttgcgccc
     3181 gtggcacttc ttatacttct tcccgctgcc gcacgggcac ggatcgttcc gtccgatctt
     3241 ggcggcccgg ggtgctgcgc cgcccaccgg gctgcgcggc cggtccccat cccctccctg
     3301 ccgggtgacg cggtaggccg gccgggccgg ctgaacgggc accgcggcgg ccggctgcac
     3361 ctggacgagg tacaggaact tgacggtctc ctcctgcacg ctgtgcttca gttgctcgaa
     3421 gagcgcgtag ccctcttttt ggtattcggt gagcgggttg gcctggccgt aggcgcgcag
     3481 cccgatcccc tcgcgcagcg cgtccatggc atacagatgg tcgatccatt tccggtccag
     3541 ggtctgcagc aggatcaacc gctccacctc gcgcatcgtc tccgggccga cggatgcgac
     3601 cttggcctcg tacgcagcca gcgcagcggt gcggatctgc tccttgaccc ccgccgcatc
     3661 cagcttgcgc agcgaatcct ccgagagccc gggaggcagc gggacgaacc cccggatttc
     3721 ctcgagcaac ccggcaaggt cccactcctc cggccgcgcc tccgcagagc agaatccctc
     3781 gaccaggtcg ccgacgaggc tctcgatgaa gttcagcacg ttctcccgga cgccggaccc
     3841 ctcgaggatc ttgcggcgct cggcgtagat gacctgccgc tgcacgttca tcacgtcgtc
     3901 gtactcgagc acgtgtttcc gaacatcaaa gtgatacgat tcgatcttct tctgcgcggt
     3961 ctcgatctgc cggctcagca tcttgtgctc gagcgggacg ttgtcctcga cgatccatcc
     4021 gatcaaccgc tccacctcgc gcatcgtctc cgggccgacg gatgcgacct tggcctcgta
     4081 cgcagccagc gcagcggtgc ggatctgctc cttgaccccc gtcgcatcca gcttgcgcag
     4141 cgaatcctcc gagagcccgg gaggcagcgg gacgaacccc c
//
LOCUS       DASQGH010000049         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17175, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000049 DASQGH010000000
VERSION     DASQGH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17175"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VEW91_00855"
     CDS             complement(<1..980)
                     /locus_tag="VEW91_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90163.1"
                     /translation="MSKNPLLSERGDRQHLALGTTEPAVRARLERLEKDLVPARLWRG
                     DPSLWAADPKVQASIRGALGWLHVADKMEEVLDDLKGFAREVGAAGFRHVVHMGMGGS
                     SLAPLVFQRTFPVGAQALPVTVLDTTDPATIVHLERAVPLAQTLFIIASKSGTTAEPA
                     AFGEYFYAKLQALKGQRAGENFVAITDPATPLVEEGRRRHYRRVFLNFPDIGGRYSAL
                     SYFGLVPATLMGVDVAGLLDRALHLVRACASCVPTAENPGIVLGAVMGELARRGRDKV
                     TLLVAEPVASLGMWLEQLMAESTGKAGTGLLPVAGEPLGDPGVYGEDRLFA"
     gene            complement(977..1336)
                     /locus_tag="VEW91_00860"
     CDS             complement(977..1336)
                     /locus_tag="VEW91_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HYM90164.1"
                     /translation="MIRLKRVYDPPSPKDGTRVLVDRVWPRGLSSAKIKIGAWMGDLG
                     PSTPLRKWFAHDPAKWEVFRTRYLRELAGKRSMLDQLSACARGGRLTLVYGARDPLRN
                     QAVVIKEMLEKGTQGRR"
     gene            complement(1386..1585)
                     /gene="ssrS"
                     /locus_tag="VEW91_00865"
     ncRNA           complement(1386..1585)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEW91_00865"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1600..3384)
                     /gene="aspS"
                     /locus_tag="VEW91_00870"
     CDS             complement(1600..3384)
                     /gene="aspS"
                     /locus_tag="VEW91_00870"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYM90165.1"
                     /translation="MSSDLGEWRRTHANGSLRRADVGQDVTLMGWVQTRRDLGGLIFI
                     DLRDREGVTQVVCNPQEAPQAADAAAQVRSEYVIAVRGLAAARPAGTVNPKLPTGEVE
                     VRVRELRILNPAETPPFRIADDVVVDESVRLRYRYLDLRRPSMYRHLALRHRLTHAVR
                     DHLGKEGFLEVETPMLIKSTPEGARDFLVPSRLHPGHFYVLPQSPQLFKQLLMVAGVE
                     RYFQIARCFRDEDLRADRQPEFTQVDIEMSFIGVDDVLRLTEGMVAATVRDALGVEVP
                     VPFPRLTYAEAMDRYGTDKPDIRFGLEVVDCSDLVQDSGFQVFRRVLESGGAVRGFCA
                     PGAGGLSRREIEQLEEAARAAGAPGLVPVHLDRDGARGAVVRHLSPESLATLRERFQA
                     KAGDLLLFVADAPRAASAALGRLRLDLGRQLGLVPDRLGFLWVTEFPLLERGQDGHRL
                     NAVHHPFTSPMDEDLPLLDTDPLAVRANAYDLVLNGVELGGGSIRIHRREVQSRMFGL
                     LGISPQDARERFGFLLDAFQYGAPPHGGIALGLDRMVMVLAGQETIREVIAFPKTQSA
                     ADLMTGAPSPVDPAALDEVHIRLKLPPP"
     gene            complement(3381..>4499)
                     /gene="hisS"
                     /locus_tag="VEW91_00875"
     CDS             complement(3381..>4499)
                     /gene="hisS"
                     /locus_tag="VEW91_00875"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYM90166.1"
                     /translation="GTDIVDKEMYTFPDRGGRSLSLRAEGTAPVMRAFLEHTLGAGGL
                     PVKVYYICPIFRYDRPQAGRYRQHIQFGAECIGAPDAAADAEVLSLAVRLVQSLGLVR
                     FEVHLSSVGDEVCRPRYLTALRAYYRPHLADLCDDCRRRFEIAPMRLLDCKREADQRL
                     ARQAPRMLDYLCDPCRAHLGSVRAHLDAMGVAYTIDPLIVRGLDYYTRTAWEGVSETL
                     GAQNVIFGGGRYDGLAAQLGGRPTPGVGFGMGIERLLLTLEREGVALPAERGPEVYIA
                     TAGAGASEALALADRLRGAGIATDLEVMARGLRAQLKQADRLGARFVLLLGEDEIRRG
                     VAAIREMATGTQEEAPLDALPEVLAARLRAETGGKARG"
BASE COUNT          680 a         1655 c         1521 g          643 t
ORIGIN      
        1 gcgaacagcc ggtcctcgcc gtagaccccc ggatccccga gcggctcgcc ggccaccggc
       61 aggagcccgg tgccggcctt gccggtgctc tcggccatga gctgctcgag ccacatcccg
      121 agcgaggcga cgggctcggc caccagcaat gtcaccttat cgcgtccccg ccgggccagt
      181 tcgcccatca ccgcgccgag cacgatgccg gggttctccg cggtgggcac gcacgaggca
      241 caggcacgca ccaggtggag ggcccggtcg agcaggcccg ccacatccac gcccatgagc
      301 gtcgcgggga ccaggccaaa gtaggagagc gccgagtacc ggccgccgat gtcgggaaag
      361 ttgaggaaca cccggcggta gtgacgtcgc cggccctcct cgaccagcgg ggtcgcggga
      421 tcggtgatgg cgacaaagtt ctcgccagcc cgctgcccct tgagggcctg gagcttggca
      481 tagaagtact cgccgaacgc cgcgggttcc gccgtcgtcc ccgacttgct ggcgatgatg
      541 aacagggtct gcgcgagcgg caccgcgcgc tccaggtgta cgatcgtcgc cgggtcggtg
      601 gtgtcgagga cggtgaccgg cagggcctgc gcgcccacgg ggaaggtccg ctgaaacacg
      661 aggggcgcga ggctgctccc gcccatcccc atgtgcacca cgtgccggaa tccggcggcc
      721 cccacctccc gggcgaaccc cttgaggtca tcgaggacct cctccatctt gtcggccacg
      781 tggagccagc cgagggctcc ccggatgctc gcctggacct tcgggtcggc cgcccagagg
      841 ctgggatccc cgcgccacag ccgcgccggc accagatcct tttccaatcg ctccagccgc
      901 gcgcggaccg caggctcggt ggtccccagg gcgaggtgct gccggtcccc ccgctcggac
      961 agcagcggat tcttgctcat cgccggccct gcgtcccctt ctccagcatc tccttgatga
     1021 caaccgcctg gttgcgcagg gggtcgcgcg ccccgtacac gagggtgagg cgcccgccgc
     1081 gcgcgcaggc ggaaagctgg tcgagcatgg accgcttccc ggccaactcc ctgaggtagc
     1141 gcgtgcggaa gacctcccac tttgcggggt cgtgggcgaa ccacttgcgc aagggtgtgc
     1201 tcggtccgag atcgcccatc caggcgccga ttttgatctt cgcgctggac agccctcggg
     1261 gccacacccg gtcaacgagc actcgggttc cgtccttcgg agaaggcggg tcgtaaaccc
     1321 gcttgagccg gatcataaac tccgaccttc cccgcctgcg gggcgatctg gtgcgtggga
     1381 atgcaatggc ccccgctgtg ccgccgcccg cagtttccga acctgctctc gcaggtgggt
     1441 gtcctcccga gcggtttcgg ctagccccct cctgcccgtg taggcagccg agggtttcgg
     1501 ggcctgtcgt ctccgtccgg agccggactc cccttgtatc actgttggct cggaactacg
     1561 cggcttgcgc acaccgcagg agccaacttg atcatagcat cagggagggg gcagcttcaa
     1621 ccggatgtgc acctcgtcaa gcgccgctgg atccaccggg gacggggcgc cggtcatcag
     1681 gtccgcggcg ctctgggtct tgggaaacgc gatcacttcc cggatcgtct cctggccggc
     1741 caggaccatc accatccggt ccaggcccag cgcgatcccg ccgtgggggg gcgccccgta
     1801 ctggaaggca tcgaggagga acccaaaccg ctcccgcgcg tcctgcgggc tgatcccgag
     1861 caggccaaac atgcgggatt ggacctcccg gcggtggatc cggatgctcc cgccgccgag
     1921 ctccacgccg ttcagcacca gatcgtacgc gttggcccgc accgcgagcg gatccgtgtc
     1981 gaggaggggg agatcctcgt ccatcggaga cgtaaacggg tggtgcacgg cattcagccg
     2041 gtggccgtcc tggccgcgct cgagcagcgg aaactccgtg acccagagga accccagccg
     2101 gtccggcacg aggcccagct ggcggcccag atccagccgg agccggccga gcgccgcgct
     2161 ggccgcccgg ggcgcgtcgg cgacaaacag caacagatcg cccgccttag cctgaaaccg
     2221 ctcccgcaga gtcgcgagcg attccggcga gaggtgccgg accacggcgc cccgcgcccc
     2281 gtcccggtcg aggtgcacgg ggacgagccc gggggcgccc gccgccctgg cggcttcctc
     2341 gagttgctcg atctcccggc ggctcaggcc gccggctccc ggcgcgcaga acccgcggac
     2401 cgcgccgccc gattccagga ccctcctgaa cacctggaag ccgctgtcct gcacgaggtc
     2461 ggagcaatcg acgacctcga gaccgaaccg gatgtccggc ttgtcggtgc cgtaccggtc
     2521 catcgcctcg gcgtacgtga ggcgcgggaa cgggaccggg acctccaccc cgagcgcatc
     2581 ccgcacggtc gccgccacca tgccctcggt gagccggagg acgtcatcga cgccgatgaa
     2641 cgacatctcg atatcgacct gcgtgaactc cggctggcgg tcggcccgaa gatcctcgtc
     2701 tcggaagcac cgcgcgatct gaaagtaccg ctccacgccg gccaccatca atagctgctt
     2761 gaacaactgc ggggactgcg ggaggacgta gaagtggccc ggatgcaggc ggctcgggac
     2821 cagaaagtcg cgcgcgccct ccggggtgct cttgatcagc atgggggtct cgacctcgag
     2881 aaagccttcc ttgcccaggt ggtcgcgcac cgcgtgggtc aggcggtgac ggagcgccag
     2941 gtggcggtac atcgagggac gccggaggtc cagataccgg taccggagcc tcacggactc
     3001 gtccaccacg acgtcgtcgg cgatgcgaaa cggcggggtc tcggccgggt tgaggatgcg
     3061 caactcccgg acccgcacct ccacctcgcc ggtcgggagc tttggattga ccgtgcccgc
     3121 cggacgcgcg gccgcgagtc cccgcaccgc gatgacgtac tcgctccgca cctgggcggc
     3181 cgcgtccgcg gcctggggag cctcctgcgg attgcagacc acctgggtca ccccctcccg
     3241 gtcgcggagg tcgatgaaga tgaggccccc caggtcacgg cgggtctgca cccaccccat
     3301 gagcgtgaca tcctgcccga catccgcgcg gcgcaggctg ccgttggcat gggtccgccg
     3361 ccactcgccg aggtcgctgc tcatccgcgc gcctttcccc cggtctcggc ccgcagacgg
     3421 gcggccagca cctccgggag ggcgtccagg ggcgcttcct cctgggtccc cgtcgccatc
     3481 tcgcggatcg ccgccacgcc gcgccggatc tcgtcctcgc cgagcagcag cacgaacctc
     3541 gcccccaggc ggtcggcctg cttcaactgc gcgcgcagac cacgggccat cacctccaga
     3601 tccgtcgcga tgccggcgcc gcggagccgg tcggccaggg ccagtgcctc ggaggccccc
     3661 gcgcccgcgg tggcgatgta cacctccggg ccgcgctccg cgggcagcgc gacgccctcc
     3721 cgctcaagcg tgagcagcag ccgctcgatt cccatgccga agccgacgcc cggagtcggg
     3781 cgtcctccga gttgcgcagc aagcccgtcg taccggccgc cgccgaagat gacgttttgc
     3841 gcgcccagcg tctcggagac cccctcccat gcggttcggg tgtagtagtc caggccgcgg
     3901 acgatgagcg ggtcgatcgt gtaggccacc cccatcgcgt ccaggtgcgc gcggacgctc
     3961 ccaaggtgtg cccggcacgg gtcgcacagg tagtccagca tgcgcggagc ctggcgggcg
     4021 agccgctggt ccgcctcgcg cttgcaatcg agcagccgca tgggggcgat ctcgaaccgc
     4081 cgccggcagt cgtcgcacag gtccgcgagg tgcgggcggt agtacgcccg caacgcggtg
     4141 aggtagcggg gccggcagac ctcgtcgccg acgctgctga ggtggacctc aaaccgcacc
     4201 aatccgaggg actgcaccag ccggaccgcg aggctcagca cctcagcgtc cgccgccgca
     4261 tcaggcgcgc cgatgcactc cgcaccaaat tggatgtgct ggcggtaccg gccggcctgc
     4321 ggccggtcgt accggaagat cgggcagatg tagtacacct tgaccggcaa tcccccggcc
     4381 cccagggtgt gctcgagaaa ggcccgcatt accggtgcgg tcccttcggc gcggagcgac
     4441 aggctgcgcc ccccccggtc gggaaacgtg tacatctcct tgtcgacgat gtccgtccc
//
LOCUS       DASQGH010000050         1793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54004, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000050 DASQGH010000000
VERSION     DASQGH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54004"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..405)
                     /gene="rplL"
                     /locus_tag="VEW91_00880"
     CDS             complement(10..405)
                     /gene="rplL"
                     /locus_tag="VEW91_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYM90167.1"
                     /translation="MAEKMTIDKIVEEIGGLTALDLSKLVKALEEKFGVSAAAPMVAM
                     APAGGAAAGGAPAAAEEQTEFDAILSSIGDKKIQVIKVVRELTGLGLKEAKDLVDGAP
                     KPVKEKVSKQEAATIKAKLTEVGATVEIK"
     gene            complement(427..1053)
                     /gene="rplJ"
                     /locus_tag="VEW91_00885"
     CDS             complement(427..1053)
                     /gene="rplJ"
                     /locus_tag="VEW91_00885"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYM90168.1"
                     /translation="MANPKPEKVAAIQDLERRLRASPVVILTDYRGLTVDEIGTLRGR
                     LREANVEYRVAKNTLLAIAAERCGVADLSRFLTGPTAVAFGRDDPGVPARVLQEFIRQ
                     YRKLEIKGGVVDGEAVDADGVRLLATLPSRLELLARALGTMQAPLRGLVTVLSAPARG
                     LVTALDAVRAQREGAGDGTPAEAGAGEEAGAPPAGAPEASGQPEASGQ"
     gene            complement(1306..>1793)
                     /gene="rplA"
                     /locus_tag="VEW91_00890"
     CDS             complement(1306..>1793)
                     /gene="rplA"
                     /locus_tag="VEW91_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HYM90169.1"
                     /translation="FAKGDKVKEAQEAGADYVGLEEYVEKIQGGWLDFDVAAAAPDVM
                     NVVGRLGRVLGPRGLMPNPKAGTVTFDLARAVREIKAGKIEYRVDKAGILHVPVGKAS
                     FSEQALLENFSELMSAVVRAKPASSKGVFLRSISLSTTMGPGIPIDPSKTGALAAAGA
                     A"
BASE COUNT          245 a          695 c          550 g          303 t
ORIGIN      
        1 ggaacaaagc tacttgatct cgacggtcgc cccgacctcg gtcagcttgg ccttgatcgt
       61 ggccgcctct tgcttggaga ccttctcctt gacgggcttg ggcgccccgt cgacgaggtc
      121 cttggcctcc ttcaggccga ggccggtcaa ctcccggacc accttgatca cctggatctt
      181 cttgtcgcca atgctcgaca ggatggcgtc gaactcggtc tgctcctccg ccgccgcggg
      241 ggcgcctccc gccgccgcgc cgcccgcggg ggccatcgcc accatcgggg ccgccgcgct
      301 cacgccaaac ttctcctcga gcgccttgac cagcttcgac agatccagcg cggtcaggcc
      361 cccgatctcc tccacgatct tgtcgatggt catcttctcc gccatgggtc tccctcccac
      421 tccgtcctat tgtcccgacg cttccggctg tcccgacgcc tctggggccc cggccggggg
      481 cgcgccggcc tcctcccccg cgcccgcctc cgcgggggtc ccgtctccgg ctccctcgcg
      541 ctgggcccgc accgcatcga gcgccgtcac gaggccccgg gcgggggcgc tgagcaccgt
      601 cacgagcccc cggagcggcg cctgcatcgt gccgagcgct ctcgccagca gttcgagccg
      661 ggacggcaac gtcgccagca ggcggacccc gtcggcgtcc accgcttcgc cgtcgaccac
      721 cccgcccttg atctccagct tccgatactg ccgaatgaac tcctgaagga cgcgggccgg
      781 cacgccgggg tcgtcccggc cgaacgccac cgcggtcggg ccggtgagga accgcgagag
      841 atcggcgacg ccgcaccgct cggcggcgat ggcgagcagg gtgttcttgg cgacccggta
      901 ttcgacgttg gcttcccgga gccgcccccg cagcgtgcca atctcatcca cggtgaggcc
      961 ccggtaatcg gtcagaatga ccaccggcga cgcccggagg cgccgctcga gatcctggat
     1021 tgccgcaacc ttttccggct tcgggttggc cattcgctcc tccaggcccc cccgttcggg
     1081 ggacccccat gcgggtggca cgccagcccc ctcttcagtg ggtaaacata aaggagaccc
     1141 gcgtagacct agagtctcct ctcaagagcc tcgacgggcg gtcagccgac ctttaggcgc
     1201 gttccgcgca cccgctgtct acggcacctg cctgtatggt gatcccgttc gatcccagcg
     1261 cccgagtagt atactaccgg cccgccggca agtcaaacgc aggggctacg ctgcgcctgc
     1321 cgccgcgagc gctccggtct tggacgggtc gatcgggatg cccgggccca tggtcgtgga
     1381 caggctgatg ctccggagaa acaccccttt gctcgaggcc ggcttcgccc ggaccacggc
     1441 gctcatcagc tcggagaagt tctcgagcag cgcctgctcc gagaaggacg ccttgcccac
     1501 cgggacgtgg aggatgccgg ctttgtcgac ccggtattcg atcttcccgg ccttgatctc
     1561 gcgcacggcg cgggcgaggt caaaggtcac ggtcccggcc ttcggattgg gcatgaggcc
     1621 ccgcgggccg agcacccggc ccagccggcc gacgacgttc atcacgtccg gggccgccgc
     1681 cgccacgtcg aagtcgagcc atcccccctg gatcttctcc acatactcct cgagccccac
     1741 atagtcggcc ccggcctcct gggcctcttt caccttgtcc cccttggcga aca
//
LOCUS       DASQGH010000051         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3012, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000051 DASQGH010000000
VERSION     DASQGH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3012"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VEW91_00895"
     CDS             <1..273
                     /locus_tag="VEW91_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HYM90170.1"
                     /translation="GGIVAVERGARVLEIPLPLGGVMAPRTLRAIAEEARGLYAFLRA
                     RGYPHIDPHYTLLFLPLDSLPDVRLTYRGVWDVRRGKVLIPREDLC"
     gene            complement(274..468)
                     /locus_tag="VEW91_00900"
     CDS             complement(274..468)
                     /locus_tag="VEW91_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90171.1"
                     /translation="MRRLRRWQFPQIEFRRLFTRCDRCGRLELPRLAYIRGSEALCTE
                     CYHAKYLTQSPEQEPLDTSV"
     gene            complement(511..696)
                     /locus_tag="VEW91_00905"
     CDS             complement(511..696)
                     /locus_tag="VEW91_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90172.1"
                     /translation="MVWYRCSCGHITEIEPHVGEVVSVYHLHAQTGVDRSALVRMEPL
                     LDSVPEPALATAGPRPK"
     gene            complement(803..2248)
                     /locus_tag="VEW91_00910"
     CDS             complement(803..2248)
                     /locus_tag="VEW91_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYM90173.1"
                     /translation="MTAYRNYIGGEWRESSGGLFEVNSPADGSFVYRAQRSTAAEMRD
                     AIMAAREAFETTEWRDDANARTQALFKFSAAVHDRHEALAPLLTRESGKPYPVSRGEA
                     LRLSETTSYFAGLTRWIFGRSQVPQPNSISLIMREPVGVVGIIVPWNAPLALLARALA
                     PALAAGNAVVVKPASYTAGSTVELAKIIEGIPEIPRGIVNFVTGPGDPVGAELARHQH
                     VDMISFTGDTSTGREIMRLAATNLKKVSLELGGKSPTIVFADANFDRAIRGALNAASF
                     YNAGQICIAGTRVLVEASIHDAFVDRIREMVPKMKVGPGWEKGVEVGPVISQSQLEKV
                     TGYIEQGKREAVLVAGGHRLTDGALAKGYFIAPTVFDQMRPDARIAQEEIFGPVMGIT
                     TFQDLDEAIRLANNTVYGLVAAVWTRDINKAMKIATRVRAGSVWVNTFGKMFQSTEMG
                     GFKQSGVGRQYGLEGLFEYTELKHVNIQIEK"
     gene            complement(2274..>2819)
                     /locus_tag="VEW91_00915"
     CDS             complement(2274..>2819)
                     /locus_tag="VEW91_00915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM90174.1"
                     /translation="NIVGALACTTLLDVMGHLQRGRVDVGFLGGAEVDRFGNLNTTWA
                     QEGERRVRLPGSGGAADIAALSRRTVVIMHHERRRFHARVRYLTSPGYGSGPGWRETV
                     GLARGGPARVITTLGVFGFDPASREMVLESLHAGVTLDEVRAHTGWPVRVSPDLRETP
                     EPSGEALAALRRFDPGGVWTG"
BASE COUNT          413 a         1015 c          924 g          467 t
ORIGIN      
        1 ggggggatcg tcgccgtgga gcggggcgcg cgggtgctgg agatccccct cccgctcggc
       61 ggcgtgatgg ccccgaggac tctgcgggcc atcgccgagg aggcgcgtgg gctgtacgca
      121 ttcctgcgcg cacgcggata cccgcacatc gacccgcact acacgctgct cttcctaccg
      181 ctcgactcgc tcccggacgt gcgtctcacc taccggggcg tgtgggacgt ccggcgtggg
      241 aaggtgctca tcccccgcga ggacctctgc tagctaaacg ctcgtgtcga ggggttcctg
      301 ttcgggtgac tgcgtgaggt acttcgcgtg gtagcactcg gtgcacaggg cttcgctccc
      361 cctgatgtag gccagcctgg gcagttccag gcggccacag cgatcgcacc gcgtgaacag
      421 acggcggaac tcaatctgag gaaactgcca gcgccggagc cgtcgcatct cccgcgatct
      481 tccccccatc gtgtcccggg tcgtaccgtg ctacttgggc cgcggacctg ccgtggcgag
      541 ggcgggttcc gggaccgagt ccagaagcgg ctccatccgg accagggcgc tcctgtcgac
      601 gccggtctgg gcgtgcagat ggtagaccga gacgacctct ccgacgtgag gctcgatctc
      661 cgtgatgtgc ccgcatgagc agcggtacca gaccatcgcc atgctccccc gtaaggtatg
      721 gatgcccatc ttccctgcgg cgcaggacat ccggcgccgc acgccccgtg ggccggcggc
      781 ctggggcgca gcgggtgcgg ggctactttt cgatctggat gttcacgtgc ttcagttcgg
      841 tgtactcgaa caggccttcg agtccgtact ggcggccgac ccccgactgc ttgaacccgc
      901 ccatctcggt cgactggaac atcttgccga aggtgttgac ccatacggac ccggctctga
      961 cgcgcgtggc gatcttcatc gccttgttga tatcgcgggt ccacaccgcg gcgaccaggc
     1021 cgtacaccgt gttgttggcc agccggatcg cctcgtcgag gtcctggaac gtggtgatgc
     1081 ccatcaccgg accgaagatc tcctcctggg cgatccgggc gtcggggcgc atctggtcaa
     1141 acacggtggg cgcgatgaag taccccttgg cgagcgcgcc gtcggtgagc cggtggccgc
     1201 cggccaccaa caccgcctcg cgcttgcctt gctcgatgta cccggtcacc ttctccagtt
     1261 gcgactgcga gatcaccggg ccgacctcca cgcccttctc ccacccgggc ccgaccttca
     1321 tctttgggac catctccctg atccggtcga cgaaggcgtc gtggatgctg gcctcgacga
     1381 gcacgcgggt gccggcgatg cagatctgcc cggcgttgta gaatgaggcg gcattcaaag
     1441 ccccccggat cgcccgatcg aagttggcgt ccgcaaacac gatcgtgggg gacttgccgc
     1501 cgagttccag ggacaccttc ttgaggttcg tggcggcgag gcgcatgatc tcgcgccccg
     1561 tcgacgtgtc cccggtgaac gagatcatgt ccacgtgctg gtggcgggcc agttcggcgc
     1621 ccaccggatc ccccgggccg gtgacgaagt tgacgatccc ccgggggatc tcgggaatgc
     1681 cctcgatgat cttcgcaagt tcgaccgtgg accccgcggt ataactggcc ggcttcacca
     1741 ccacggcgtt ccccgccgcc agggcggggg cgagcgcgcg cgccaggagc gcgagggggg
     1801 cgttccacgg cacgatgatc ccgaccacgc cgaccggctc ccgcatgatc aggctgatac
     1861 tgttcggctg cggcacctgc gaccgtccga agatccaccg ggtgaggccg gcgaagtagc
     1921 tcgtcgtctc cgacagcctg agcgcctcac cccggctgac cgggtagggc ttgccgctct
     1981 cccgcgtgag caggggggcc aaggcctcgt gccggtcgtg cacggccgcg gagaacttga
     2041 agagggcctg ggtgcgcgcg ttcgcgtcgt cccgccactc ggtcgtctcg aacgcctccc
     2101 gcgcggccat gatcgcatcg cgcatctccg ccgccgtcga ccgctgcgcc cggtacacga
     2161 acgagccatc tgccggactg ttgacctcaa agaggccgcc cgacgactcc cgccattctc
     2221 cgccgatgta gttcctgtac gcagtcacct ctgccatggc cctccccctc ccatcagcct
     2281 gtccacactc cacctgggtc gaaccgccgc agcgccgcga gtgcttcccc gcttggctcc
     2341 ggcgtctccc ggaggtccgg cgacacccgc acgggccagc ccgtgtgcgc gcggacctcg
     2401 tccaatgtca ccccggcatg cagactctcc agcaccatct cgcgcgacgc cgggtcgaag
     2461 ccgaataccc cgagggttgt gatcacgcgc gccggaccac cccgcgcaag cccgacggtc
     2521 tcgcgccacc cgggaccgct cccgtacccc ggcgaggtga ggtagcggac ccgcgcgtga
     2581 aaccggcggc gctcgtggtg catgatcacc acggtccgcc gcgagagcgc ggcgatatcc
     2641 gcggccccgc cgctgccggg aagacggacg cggcgctcac cctcctgcgc ccacgtggtg
     2701 ttgaggttgc cgaaccgatc cacctcggct cccccgagga acccgacgtc cacgcggccg
     2761 cgctggaggt ggcccatgac gtcgaggagg gtggtgcacg cgagcgcgcc gacgatgtt
//
LOCUS       DASQGH010000052         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3013, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000052 DASQGH010000000
VERSION     DASQGH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1131
                     /locus_tag="VEW91_00920"
     CDS             262..1131
                     /locus_tag="VEW91_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYM90175.1"
                     /translation="MTQLRQRMMEDMQLRGFSARTQECYVAAVRQLAAHYHRSPDQLT
                     EEDLRQYFLYLANEKKVARATATIALCGIKFFYEQTLQCQWTTLRFVRPPREKKLPVV
                     LSRDEVRRILAEVRIPVYRACLTTIYACGLRLLEGAHLQVADVDSGRMVLHIHGKGKQ
                     DRYVPLPAPTLASLRTFWRTHRSPAWVFPAPTRHGLAHSLAHGGGPVTRSSLQSAFRR
                     ALRRTGIAKRAHVHTLRHSYATHLLEAGVNLRLIQENLGHRSARTTQVYTHLTREAQA
                     TLTDPLNQLMQGL"
     gene            1148..2257
                     /locus_tag="VEW91_00925"
     CDS             1148..2257
                     /locus_tag="VEW91_00925"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HYM90176.1"
                     /translation="MLEVADIVRLHGAAYRARYGQALGASHRRALRDLAACRTPACGG
                     HVYQCDHCGETRYTYPSCGNRHCPKCHRDQTERWLAAQRAHLLPCAYFLLTFTLPEAL
                     RALARTHPKKVYGLLMQCAAAALQTLALDPRYLGARLGCLAVLHTWTRALLYHPHVHL
                     LVTAGGLAADGAGWIPSKHRSYLVPVQALSPIFRAKMCAGLQKAGVLAQVPPAVWQQG
                     WVVHCQPAGRGEQVLAYLDRSLCRLAITNSRLERLADGQVTFRYRDNRTHKMRRVTLP
                     GVEFLHRFLQHVLPRGCTKVRSYGLWSPTCRPQLAQAQALLAVPEAPASADPAPRTPA
                     APPPASAPAAARCPRCRVGRLLLVEVLRPHRSRSP"
BASE COUNT          400 a          995 c          811 g          429 t
ORIGIN      
        1 agcgtcccac cgccagcaaa gcgagccgcg cttgcaggcg gtcggtgaaa tcgaagcgtt
       61 tcggcatgaa ggctaacgtt cgcgcgcagc ggggcacgga gcgcggccgc taaattgctg
      121 ggttgaacta aatccgcctt cggcggatag atagggactc tcgcctcgta atcgtctccc
      181 gccgttcgct tcaggccgtc cttcgcgtca gtatcccttt ccgggagagg gtaagcatcg
      241 ctcccggaaa ggagatacgt gatgactcag ttgcgtcagc ggatgatgga ggatatgcaa
      301 ctgcggggat tttcggcccg cacgcaggag tgctacgtgg ccgccgtgcg ccaactggcg
      361 gcgcactacc accgcagccc ggatcaattg accgaggagg acctgcgcca gtacttcctg
      421 tacctggcca acgagaagaa ggtggcccgc gccaccgcca cgattgcgct gtgcgggatc
      481 aagttcttct acgagcagac gctccagtgc cagtggacga ccctgcggtt tgtccggccg
      541 ccccgggaga agaagctgcc cgtcgtcctc agccgggatg aagtgcgccg catcctcgcg
      601 gaggtgcgca tccccgttta ccgggcctgc ctgaccacca tctatgcctg cgggctgcga
      661 ctgctggaag gcgcgcacct gcaggtggcc gatgtggaca gcgggcggat ggtgctgcac
      721 atccacggca aaggcaaaca agaccggtac gtcccgctgc ccgcccccac gctcgcgtcg
      781 ctgcgcacct tctggcgcac gcatcggtcg cccgcctggg tgttcccggc gcccacccgg
      841 cacggtctgg cccatagcct cgcccacggg ggcggtccgg tcacccgcag cagcctccag
      901 agcgcgttcc ggcgggcgct ccgacggacc ggcatcgcca agcgggccca cgtccacacg
      961 ctgcggcatt cgtatgccac gcacttgctc gaagccggcg tcaacctccg gctcattcag
     1021 gagaacctcg ggcaccgcag cgcccgcacc acgcaggtct acacccatct gacgcgagag
     1081 gcgcaggcca ccctcaccga tccgctgaac cagctcatgc aaggcctctg atccttcggc
     1141 ggcctgcatg ctggaggtgg cggacatcgt ccgcctccac ggggcggcgt accgcgcccg
     1201 ctacggccag gccttggggg ccagccaccg ccgggccctg cgggatctcg cagcctgccg
     1261 cacgccggcc tgcggcggcc atgtgtacca gtgcgatcac tgcggcgaaa cgcgctatac
     1321 ctatccctcc tgcggcaacc gccactgtcc gaagtgtcac cgggatcaga ccgagcgctg
     1381 gctcgccgcg cagcgcgccc acctgctccc ctgtgcctac ttcctgctga cctttaccct
     1441 gcccgaggcc ttgcgcgccc tggcgcgaac ccatccgaaa aaagtctatg gcctgctgat
     1501 gcagtgtgcc gccgcggcgc tgcagacgct tgccctcgat ccccgctatc tcggggcacg
     1561 gctcggctgc ttggcggtcc tgcatacctg gacgcgggcg ctgctgtatc atccccacgt
     1621 ccacctgctc gtcaccgcag ggggcctcgc cgccgatggc gccgggtgga tcccgtcgaa
     1681 gcaccgcagc tatctggtcc cggtccaggc cctgtcgccg atcttccgcg cgaagatgtg
     1741 cgcgggcctg caaaaggccg gggtcctcgc gcaggtcccc ccggcggtgt ggcagcaagg
     1801 ctgggtcgtc cattgccagc ccgcgggtcg cggggagcag gtgctcgcct acctcgaccg
     1861 ctccctctgc cgcctcgcga tcaccaatag ccgcctggaa cggctcgcgg acggccaggt
     1921 caccttccgt tatcgcgaca accggaccca caagatgcgc cgggtcaccc tcccgggcgt
     1981 cgaattcctc caccgcttcc tccagcatgt gctcccgcgt ggctgcacca aggtccgctc
     2041 ttacggcctc tggagcccca cctgccgccc ccaactcgct caggcccagg cgctcctggc
     2101 cgtgccagag gcccccgcgt ccgccgaccc ggctccccgc accccggcgg ctccgccgcc
     2161 ggcctccgcg cccgcggcgg cgcgctgccc gcggtgtcgg gtgggccgcc tgctcctcgt
     2221 cgaggtcctg cggccgcacc ggagccggtc accatgaccg cgtctggtgg ccatgccgtc
     2281 cgctccacgg cacgcgctct ggccgcgtgt cagcggggtc gtctgtccct caggcccctc
     2341 gcgacgcgat cctgccggct gagagcccgt gctgtcgcca cccggaggcc gattcccggc
     2401 ttccaccgca cgggccacac gctgcaccgc tcgtcgttcc ctggcccccc acacagcgca
     2461 ccgcattgaa actccaaatc cccacggcca tccaccggat ttagttcaac cccggatttt
     2521 gtcgcggctc cgcgcgacaa gaatccttaa tttgttagtg ctattgtgtc acaaaattat
     2581 ggccgccctc cggaaccgag agggatggcg gctcgcatca cctagagggc aaaag
//
LOCUS       DASQGH010000053         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45544, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000053 DASQGH010000000
VERSION     DASQGH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45544"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VEW91_00930"
     CDS             <1..616
                     /locus_tag="VEW91_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYM90177.1"
                     /translation="KRAGGDDHGGVLPAHDTHFLRPRRRAGPTRDIREACWTDPENSG
                     PVTIRPMREPDVSSCAAIMHASPLWVRYGITTLEAARRALGDMLAGTCLGFVAEDAGG
                     VIGFVVYAVEGTFIHSGYVRSVAVARDAQRRGVGGRLMDAAEAAILGRGPNVFLLVSA
                     RNTDAQRFYERRGYRRVGEIPDYVRRGITECLYRKTRGPIEGGV"
     gene            640..2172
                     /locus_tag="VEW91_00935"
     CDS             640..2172
                     /locus_tag="VEW91_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90178.1"
                     /translation="MRGLGLVVTGLLAAGLIPGAAPLGTQAAPGKVTLTLGIDQEVVG
                     LDPNKVTAFSSFRRIDLLYNKLVTYDTDLRVVGDLAESWDTPDSRTYVFHLRKGVRFH
                     DGREMTAEDVVFTIERILDPKTASPGRSYIDAVDGVAAPDRYTVRLHLKYPLASIFSG
                     LASGNASIVEKAAVLASASGDLQKTEAGTGPFMLAEWVPDNFMRLIRNPGYFKRGLPK
                     VDEVVYRVIPEQASLLAGVRTRSLDMATISDGSVVRQAQADKALLVLQAPRLNLRIFS
                     FNTTRGPCTDARVRDAIAFAIDREAIVRAAEFGLAEVSGPVPAPAKIWALPVRQFPEY
                     HPDPARARQMLQDAGAAGASFRIVASPTYEGGLAVAQVIQSQLRAVGLVPTIESIEWG
                     TYINRWVRRDFDSMVDLRGGDPDPDRFLYRTFYSTGAVNNFLFKDAAVDRLLDRGRVH
                     LVGAERKPIYDELQRALVEKAPAIFLYTPYETQVLQPSVTGFRLIPTGALTYLEQTSI
                     ER"
     gene            2235..>2821
                     /locus_tag="VEW91_00940"
     CDS             2235..>2821
                     /locus_tag="VEW91_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90179.1"
                     /translation="MFLLIHLIPGDALQMFLGTQVAMTPAQMEELRRLFGLNNSLPLQ
                     YADWVGRLLHGDFGVSLRTGRPVLPDLLARLPVSAELATLALLVALAIALPVGVLSAL
                     RRDTAADAAIRVGGLVGLSVPNFWLATMLLLLLPGRVLPIASIGIYVRFFGNPIGNLT
                     VMLLPAVSLGVALAAVIMRYVRSALLEVLGRDYIRT"
BASE COUNT          390 a          976 c          980 g          475 t
ORIGIN      
        1 caagcgtgcc ggcggagatg atcacggagg cgtgcttccg gctcacgaca cccacttcct
       61 gcggccccgc cggcgggccg gccccacgcg cgatatccgg gaggcctgct ggacggaccc
      121 ggagaatagt gggcctgtga ccattcggcc gatgcgcgag cccgacgtgt ccagttgcgc
      181 cgccatcatg cacgcctcgc ccctgtgggt gcggtacggg atcaccacgc tggaggcggc
      241 gcggcgtgcc ctgggggata tgctcgcggg gacctgcctc ggcttcgtcg cggaagacgc
      301 cgggggcgtc atcggctttg tcgtctacgc ggtcgagggg acatttatcc acagcgggta
      361 cgtgcggtcc gtggcggtcg cacgggacgc gcagcgcaga ggggtcggag gccggttgat
      421 ggatgccgcg gaggcggcga ttctcgggcg cgggccgaac gtgtttctgc tcgtgtcggc
      481 gaggaacacc gatgcccagc ggttctacga gcgccgcggc taccggcggg tcggggagat
      541 tccggactac gtgcggcgcg gaatcaccga gtgcctgtat cgcaagacgc gcggcccgat
      601 cgagggcggt gtgtgacaag gaggtgtctc gtgcacccga tgcgtggact ggggctcgtg
      661 gtcacagggc tgctggcggc ggggctcatc cccggggccg cccctctggg gacgcaggcg
      721 gcccccggca aggttaccct gacgctcggg atcgatcagg aggtcgtggg cctggacccc
      781 aacaaggtga ccgcgttttc gtcgttccgg cggatcgacc tcctctacaa caaactggtc
      841 acgtacgaca ccgacctccg agtggtcggc gacctcgcgg agtcctggga caccccggac
      901 tcccggacct acgtctttca cctgcgtaag ggcgtgcggt tccacgacgg gcgggagatg
      961 accgcggagg acgtcgtctt cacgatcgaa cgcatcctcg atcccaagac cgcctcgccc
     1021 ggccgatcct acatcgacgc cgtggacggt gtcgcggcgc cggaccgcta cacggtgcgc
     1081 ctccacctga agtacccgct ggcctccatc ttctccgggc tggcgtccgg caacgcctcg
     1141 attgtggaga aggccgcggt cctcgcctcc gcgtcggggg acctccagaa gaccgaagcc
     1201 ggcaccggcc cgttcatgct cgccgagtgg gtccccgaca acttcatgcg gcttattcgg
     1261 aaccccggct acttcaagcg ggggctcccc aaggtggacg aggtggtgta ccgcgtgatt
     1321 cccgagcagg cctcgctgct cgccggggtt aggacgcgga gcctcgacat ggcgacgatc
     1381 tcggacggca gcgtcgtccg gcaggcccag gccgacaagg cgctgctcgt cctccaggcc
     1441 ccccgcctca acctccgcat cttctccttc aacaccaccc gggggccgtg taccgatgcg
     1501 cgggttcggg acgcgatcgc cttcgccatc gaccgcgagg cgatcgtcag ggcggccgag
     1561 ttcgggctcg cggaggtgag cggtccggtc ccggctcccg ccaagatctg ggccctcccg
     1621 gtgcggcagt tccccgagta ccatccggac ccggccaggg cccggcagat gctccaggac
     1681 gcgggggcgg ccggggcgtc gttcaggatc gtcgcgtccc ccacctacga gggcgggctg
     1741 gcggtcgccc aggtgatcca gagccagctg cgcgccgtcg ggctcgtccc aacgatcgag
     1801 agcatcgagt gggggacgta catcaaccgc tgggtcaggc gcgacttcga ctcgatggtg
     1861 gacctgcggg gcggcgatcc cgaccccgac cggttcttgt accggacgtt ctacagcacg
     1921 ggcgcggtga acaacttcct gttcaaagac gccgcggtcg atcggttgct cgaccgcggc
     1981 cgggtgcatc tggtgggcgc agagcgcaag ccgatctacg atgagctgca gcgcgcgctc
     2041 gtcgagaagg cgcccgcgat cttcctgtac acaccgtacg agacccaggt gctgcagccc
     2101 tccgtcacgg ggttccgcct catcccgacg ggggcgctca cctacctgga gcagacctcg
     2161 atcgaacgct gaccgcgttc atcgccaccc gtctgctctc cctcctcccc gtcctctggg
     2221 gggtctcgat cctggtgttt ctcctgatcc acctcatccc cggggacgcc ctccagatgt
     2281 ttctcggaac ccaggtggcg atgaccccgg cgcagatgga ggagctgcgg cgcctctttg
     2341 ggctgaacaa ctcgctgccg ctgcagtacg cggactgggt ggggcggctg ctccacggcg
     2401 actttggcgt gtcgctgcgg accggccgcc cggtgctgcc cgatctgctg gcgcggctgc
     2461 cggtgagcgc cgagctcgca acgctcgcgc tcctcgtcgc gctggcgatc gcgctgccgg
     2521 tgggggtgct ctccgcgctc cggcgggaca cggcggccga cgccgcgatc cgggtgggag
     2581 gcctcgtggg gctcagtgtc cccaacttct ggctggcgac gatgctgctg ctcctgcttc
     2641 cgggccgcgt cctgccgatc gcctcgattg ggatctacgt caggttcttc ggcaacccca
     2701 ttggcaacct gacggtgatg ctgctccccg ccgtctcgct cggcgtggcg ctcgccgcgg
     2761 tgatcatgcg atacgttcgc tcggcgctgc tggaggtcct cgggcgagac tacatccgga
     2821 c
//
LOCUS       DASQGH010000054         4526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11554, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000054 DASQGH010000000
VERSION     DASQGH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11554"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VEW91_00945"
     CDS             <1..401
                     /locus_tag="VEW91_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYM90180.1"
                     /translation="EVNRIVSRIARVPEVRQALGALQRSLGRRGNVVMEGRDIGSVIL
                     PDAQVKVFLTASLDVRAQRRQAELAANGTPMPLGEIQRIVVEDDRAATTREVAPLRRM
                     PDAVVIDSTALTVDQVVDQIVGLVERARGL"
     gene            391..1026
                     /locus_tag="VEW91_00950"
     CDS             391..1026
                     /locus_tag="VEW91_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYM90181.1"
                     /translation="MVYRLSRALIRLVLRVACGFRVEGGEHEPPTGPLLIVSNHVSDL
                     DPLVVGVTLRRPTHFMAKEELFRPPLLRWWITACGGFPVRRGEPDRGAFRTARAILER
                     GGALGMFPEGTRGSSLRDLRPPEPGAALLALRTGATILPVAVIGTDVVLPRGASRPRR
                     GTIRVRVGAPIRVDGAGNPPVRTRADRERIDALGQMYMGVIARLLGGSGGP"
     gene            1111..1953
                     /gene="ispH"
                     /locus_tag="VEW91_00955"
     CDS             1111..1953
                     /gene="ispH"
                     /locus_tag="VEW91_00955"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYM90182.1"
                     /translation="MKIIVAKHMGFCGGVRRAVDIAHKTAASTGGAVQTWGPLIHNPQ
                     VVERLEASGVLVAERHEDLGGGAFVVSAYGVEHAVLDEARRRGMTVVDATCPVVIRAH
                     ALAEKLAGEGYQVIAVGDHGHPEMVTLKELLGDRVTVVHTREEAAAVKVTGKLGVVSQ
                     TTQSQENFRQIVADLVLRVRELKVLNTLCPAITVRQEEADVMMPQIDALLVIGGHGSS
                     NTTRLAELGRARGLPTYHIETAAEISPAWFDSAGTLGVVSGASTPTWIIEAVMLRLQE
                     LGGA"
     gene            1950..3329
                     /gene="der"
                     /locus_tag="VEW91_00960"
     CDS             1950..3329
                     /gene="der"
                     /locus_tag="VEW91_00960"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HYM90183.1"
                     /translation="MTGGRSPAARPGPDHALPAIAIVGRPNVGKSALFNRLLARRHAI
                     VSDIPGVTRDRLQAPCQWAGREFLLVDTGGLVPGAAEPLTVQVRRQAERAITDAQAVL
                     FVVDASAGLTPQDQEIAAVLRRHHRPVLVVANKVDTPELAAGAYEFSALGLGDPVMVS
                     ATHGLGIGDLLDAVVAIVPDVEAAAEAEPAVGVVFLGRPNVGKSSLVNAVLGEERVVV
                     DARPGTTRDAVDTLLRYDGQPVTLIDTAGLRRGSRMASGLEYYSMRRTEEALSRADVA
                     VLVIDAQEGITDQDQRIARMVYEAGRASVIAVNKWDLVRGYTPEQIQRVTQQRLQFLG
                     DVLVVQTVATQRRGTAELMAAVFRAAQARATRIATGPLNRVIAEAIQAHAPSADATGR
                     QLHVYYATQPSTRPPAIVLFVNDPRLVTVAYERYLERRLRAEVDLAGTPVRWILRRRR
                     PAASARTTR"
     gene            3345..3944
                     /gene="plsY"
                     /locus_tag="VEW91_00965"
     CDS             3345..3944
                     /gene="plsY"
                     /locus_tag="VEW91_00965"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HYM90184.1"
                     /translation="MPLAAIILSYLVGAIPTGLIVVRVLTGEDIRRHGSGNIGTVNVL
                     RVAGAATAVIVLAVDILKGIAPVLLALRMGFAPWTVVLSGLAAIIGHNWSVFLGWQGG
                     KGIATSFGVLLGLSWQAAAVAAVVWVVAVTLTRYASLGSLLGVVSVPITLWRLHQPHE
                     YVYLGVIVAFLAIYRHRANIQRLVTGAELHITDRASLKK"
     gene            3946..>4526
                     /locus_tag="VEW91_00970"
     CDS             3946..>4526
                     /locus_tag="VEW91_00970"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYM90185.1"
                     /translation="MHVLCFGAGAIGSLVGARLAQSGVAVTLLARRDHVAAIRTRGLI
                     VEGPRGRSVCKNIDAIAWLDDLATPPDLVLLTVKAYHTAAALEVLRPLWRDPVTIVSL
                     QNGVGNEEMIAAAAGAERTFAGTITISASRPRPGVVRQHTRVGGIGVAPLDPRQDASS
                     LVALFRRAGIRAALYREYRRMKWRKLLLNLFTNA"
BASE COUNT          613 a         1575 c         1677 g          661 t
ORIGIN      
        1 tcgaggtcaa ccggattgtg tcgcggatcg ccagggtgcc ggaggtccgg caggcgctcg
       61 gcgcgctcca gcggtcgctc ggccgccggg gcaacgtcgt catggaaggc cgcgacatcg
      121 ggtcggtgat cctgcccgac gcccaggtga aggtgttcct gaccgcctcc ctggacgtgc
      181 gggcccagcg ccgccaggcg gaactggcgg cgaacggcac gccgatgccg ctcggggaga
      241 tccagcggat cgtcgtggag gacgaccggg ccgctaccac ccgcgaggtg gcgccgttgc
      301 gccgcatgcc cgacgccgtg gtcatcgaca gcaccgcgct caccgtcgat caagtcgtcg
      361 atcagatcgt gggcctggtg gagcgcgcgc gtggtctata ggctctcccg ggccctcatc
      421 cgactggtgc tccgcgtagc gtgtggcttt cgggtcgagg gcggggagca cgagcccccg
      481 accggtcccc tcctgatcgt cagcaaccac gtgagcgacc tggatcccct ggtcgtcggc
      541 gtgaccctgc ggcgcccaac ccatttcatg gcgaaggagg aactgtttcg tcccccgctg
      601 ctgcggtggt ggatcaccgc ctgcggcggg ttcccggtca ggcgggggga gccggaccgc
      661 ggggcgttcc gcacggcgcg ggcgatcttg gaacgcggtg gggcgctggg gatgttcccg
      721 gaagggaccc gggggagcag cctccgggac ctgcgcccgc ccgagcccgg cgcggccctg
      781 ctggccctcc gcaccggggc gacgattctg ccggtcgcgg tgatcggcac cgatgtggtg
      841 ctgccgaggg gcgcctcgcg gcccaggcgg gggacgatcc gcgtgcgggt gggggcgccg
      901 atccgggtgg atggggcggg gaatcccccc gtccggaccc gcgccgaccg ggagcggatc
      961 gatgcgctcg gccagatgta catgggggtg atcgcgcgcc tgctcggtgg gagcggcggg
     1021 ccgtagccgc gcggtccccg ggcctcgaca gggggacgtt tcccccctat catggggaag
     1081 gacccagccg ggtgcgcgtg gaggacgccg gtgaagatca tcgtcgccaa acatatgggg
     1141 ttttgcggcg gggtgcgccg cgcggtcgat atcgcgcaca agaccgcggc gtcgaccggt
     1201 ggggccgtgc agacctgggg cccgttgatc cataacccgc aggtcgtcga gcggctcgaa
     1261 gcgtccgggg tgctcgtcgc cgagcgccac gaggacctgg gcgggggcgc gttcgtcgtc
     1321 tccgcctacg gggtcgagca cgcggtgctc gacgaggccc ggcggcgcgg catgacggtg
     1381 gtcgacgcga cgtgccccgt ggtgatccgg gcgcacgcgc tcgccgaaaa actggcgggg
     1441 gaggggtacc aggtgatcgc ggtcggcgac cacgggcacc ccgagatggt cacgctcaag
     1501 gagctcctgg gcgaccgggt caccgtggtc catacccgcg aggaagcggc ggcggtcaag
     1561 gtgaccggca aactgggcgt cgtgtcccag acgacgcagt cgcaggagaa ctttcgccag
     1621 atcgtcgcgg acctggtgct ccgggtgcgc gagttgaagg tcctcaacac gctgtgcccg
     1681 gcgatcaccg tccggcagga agaagccgac gtcatgatgc cccagatcga cgcgctgctc
     1741 gtcatcggcg ggcacgggag ctccaacacc acccggctgg cggagctggg gcgtgcgcgc
     1801 ggcctgccca cctaccacat cgaaacggcc gcggagatct cccccgcatg gttcgacagc
     1861 gcgggcacgc tcggggtggt ctcgggcgcc agcaccccca catggatcat cgaggcggtg
     1921 atgctccggc ttcaggagct gggcggggcg tgaccggcgg gcgctcgccc gcggcccgcc
     1981 ccgggcctga ccacgcgttg ccggccatcg ccatcgtcgg ccggccgaac gtcgggaagt
     2041 ccgcgctgtt caaccggctg ctggcccgcc gccacgcgat cgtctccgac atccccggcg
     2101 tgacgcgcga tcgcctccag gccccctgcc agtgggccgg ccgggagttc ctcctcgtgg
     2161 acacgggcgg gctcgtgccg ggcgccgccg aaccactcac ggtgcaggtg cggcgccagg
     2221 ccgagcgcgc gatcacagac gcccaggcgg tcctgtttgt ggtcgatgcg tctgcggggc
     2281 tgaccccgca ggaccaggag atcgcggccg tgctgcgccg ccaccaccgg cccgtgctgg
     2341 tcgtcgcgaa caaggtggac acgcccgagc tggcggccgg ggcgtacgag ttttctgccc
     2401 tcgggctggg ggacccggtc atggtctcgg ccacccacgg gctgggcatc ggcgacctcc
     2461 tcgatgccgt ggtcgccatc gtgcccgacg tggaagccgc cgcggaggcc gaacctgcgg
     2521 ttggggtcgt gttcctcggc cgcccgaacg tcggcaagtc ctcgctcgtc aacgccgtcc
     2581 tcggcgagga gcgggtcgtc gtggacgcgc gcccggggac gacccgagac gcggtggaca
     2641 ccctgctgcg ctacgacggg cagccggtga ccctcatcga cacggcgggg ctgcggcgcg
     2701 ggagccggat ggccagcggg ctcgagtact acagcatgcg ccgcaccgag gaggccctgt
     2761 cgcgggccga cgtggccgtc ctggtcatcg acgcgcagga ggggatcacg gaccaggacc
     2821 agcggatcgc ccgcatggtg tatgaggccg ggcgcgcctc ggtgatcgcg gtcaacaagt
     2881 gggatctggt gcgcggctac accccggagc agattcagcg ggtgacccag cagcggctgc
     2941 agttcctggg ggacgtcctg gtcgtccaaa cggtggccac ccaacgccgc ggcaccgcgg
     3001 aattgatggc ggcggtcttc cgcgccgccc aggcgcgggc cacccggatc gccaccgggc
     3061 cgctcaaccg ggtgatcgcg gaggcgattc aggcgcacgc cccctcggcg gatgccaccg
     3121 gccggcagct gcacgtctac tacgcgacgc agcccagcac ccgccccccc gcgatcgtgc
     3181 tgttcgtgaa cgacccgagg ctggtcacgg tcgcgtacga gcggtacctg gagcggcggc
     3241 tccgggcgga ggtcgatctt gcgggcacgc cggtgcgctg gatcctccgg aggaggaggc
     3301 cggcggcgtc cgcgcgaacc acgcggtgac gcgaggagga gcgcatgccg ctcgcggcca
     3361 tcatcctcag ttatcttgtg ggcgcgattc caacgggcct cattgtggtc cgcgtcctca
     3421 ccggcgagga catccggcgc cacgggagcg ggaacatcgg gacggtcaac gtccttcgcg
     3481 tggccggtgc cgccacggcg gtaatcgtgc tcgcggtcga catcctcaag gggatcgcgc
     3541 ccgtcctgct cgccctccgg atggggttcg ccccctggac cgtggtcctg agcgggctgg
     3601 cggcgatcat cgggcacaac tggtcggtct tcctcgggtg gcagggcggg aaggggatcg
     3661 ccacgtcgtt cggcgtcctc ctcggcctct cgtggcaggc ggccgcggtg gcggcggtcg
     3721 tgtgggtggt cgccgtgaca ctcacgaggt acgcctcgct cggatcgctg ctgggtgtcg
     3781 tctccgtccc gatcaccctc tggcggctcc accagcccca tgaatacgtg tacctggggg
     3841 tcatcgtggc gttcctcgcc atctaccggc accgggccaa tatccagcgg ctggtgacgg
     3901 gcgcggaact gcacatcacc gaccgcgcgt cgctcaagaa ataggatgca cgtcctctgc
     3961 tttggggctg gggccatcgg ctccctggtc ggcgcccgcc tcgcccaaag cggggtggcg
     4021 gttaccttgc tcgcgcgccg cgatcacgtc gcggcgattc ggacccgcgg gttgatcgtc
     4081 gaggggccgc gcggtcggag cgtctgcaag aacatcgatg ccatcgcgtg gctcgacgac
     4141 ctggccaccc ctcccgatct cgtcctcctc accgtgaagg cctaccatac ggccgcggcc
     4201 ctcgaggtcc tccgtccgct gtggcgcgac cccgtgacga tcgtgtcgct gcaaaacggc
     4261 gtcgggaacg aggagatgat cgcggccgca gccggggccg agcggacgtt cgcggggacg
     4321 atcacgatca gcgcgagccg gccccgcccg ggggtcgtcc ggcagcacac cagggtcggc
     4381 ggcatcggcg tcgcccccct ggatcctcgc caggacgcct ccagcctggt ggcgctcttc
     4441 cggcgcgccg ggatccgcgc cgcgctgtac cgcgagtacc gccgcatgaa gtggcgcaag
     4501 ctcctactca acctcttcac caacgc
//
LOCUS       DASQGH010000055         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39870, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000055 DASQGH010000000
VERSION     DASQGH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39870"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VEW91_00975"
     CDS             complement(<1..655)
                     /locus_tag="VEW91_00975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYM90186.1"
                     /translation="MDDVEATGSSSCRCTAALLPRPLDVSRFIIGAAIAGVLLAPAVI
                     VTGPGGAPPGVAHAQVFSFPGGIPPADFRTDVEIEASAAQVWLVLTNFAAYPIWNPFI
                     YPVKGDARVGNTLEITIHPGTRSITYRATVVAMRPDRELAWFAQSVSGGFFDITYSFT
                     IEPVQANRVRLISRETHTGLASILETSLVKDVQSGLDAMTKAVRNRVELERIFMRPGE
                     "
     gene            775..1566
                     /locus_tag="VEW91_00980"
     CDS             775..1566
                     /locus_tag="VEW91_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYM90187.1"
                     /translation="MVTCPGRRRSRAPRLAGAVALLLALALGRPAPALGEDANLFDSV
                     FELFTYTRQGHGIARGTAFFIDPSGLALTNSHVVHRVQQDPEHYVLLAVVGKEFYGVE
                     ILCASPLELDPLEAAKGPVSRDIAKIRLVAPDAEFSLWGILPGAGASVLARAHQGPLP
                     IFPVLTLGDGPQERGQIRVIGFGRASQATEKTVATGTVTLTALAKDGTPVFEIQSTAR
                     PERGSSGSPVVDDQNRVVGMYTWNDTHNAESGIAISSTALAHACP"
     gene            1639..3222
                     /locus_tag="VEW91_00985"
     CDS             1639..3222
                     /locus_tag="VEW91_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HYM90188.1"
                     /translation="MTAAIDLPEEFNVSTAFLDRNLAEGRGSRVAIYSGDERITYQAL
                     ASQVNRAGNALRALGVRPEERVLLLMLDTPEAAYLFWGAIRIGAVAVPMNTALRPPDY
                     AYMLHDSRAAVLVVSEELLPAVEPALGDRGPLRHIIVAGGGTPGSGRHALRDLLGAAD
                     PRLDPARTHRDEPAFWLWSSGSTGAPKGTVHLHHDMVWAAELYARTVLEMRADDVCFS
                     VAKLFFAYGLGNALYFPFRVGAATVLYPGRFEPRLYFDLIRRYRPTLFFGVPTAYAAM
                     LAQEGPTDLGPVRMCVSAGEPLPAALFSRWKERFGVEILDGIGSTEALHIYISNRPGR
                     TRAGSSGEPVPGYAVRIVDETGRDLPAGEIGDLLVSGDSIAAGYWNKHERTKQAFRGE
                     WFVSGDKYYRDADGYYWYCGRSDDMLKVGGQWVSPAEVEATLIQHPAVLECGVVGKDD
                     EDGLSKPCAFVVLKPGHASGTPLAKELQAFVRDKIAAYKYPRWIEFVPELPKTATGKI
                     QRFRLRERLGAGRKPAPAG"
     gene            3245..4015
                     /locus_tag="VEW91_00990"
     CDS             3245..4015
                     /locus_tag="VEW91_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM90189.1"
                     /translation="MISLERDGAVGQISLHRPPANAYNQQFAEELEEAVEAVRADDGI
                     RAVVVTSTLPRFFSAGADIKMFQASTLPEKEKFILRMHEVLRKIEMTPKVFIAAITGH
                     CLGGGLEIALACDLRFAAAGKYGLGQPEITLGILPGNGGTQRLPRLIGKSRALDLMVT
                     GRTVSPDEALSLSLVDRVFPAEELAAKTREYAEALTRSAAMAVGLIKLTVTRGLELPL
                     DSGLAYEREALFRVFASADAVEGVNAFLEKRPPVFKGK"
     gene            complement(3913..>4268)
                     /locus_tag="VEW91_00995"
     CDS             complement(3913..>4268)
                     /locus_tag="VEW91_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HYM90190.1"
                     /translation="ARRLRQHTSSDAACFAEKILLVHEYRKFLFVDPGLPQALLPSDW
                     PGWDAARVFRDVYQTLEAPAARFFADAFEPPPGSRWRARAYFPLKTGGRFSKKALTPS
                     TASALANTRNNASRS"
BASE COUNT          679 a         1413 c         1514 g          662 t
ORIGIN      
        1 gttctccggg gcgcatgaag atgcgctcca actccacacg gtttctgacg gccttcgtca
       61 tggcgtcaag tccactctgg acgtctttca cgagtgacgt ttcaaggata ctggcaaggc
      121 ccgtatgggt ctcgcgcgag atcaagcgca cgcggttggc ttgaaccggt tcaatggtga
      181 agctgtaggt aatgtcgaag aagccccccg acacgctttg cgcaaaccag gccaattcgc
      241 gatcgggccg catcgcgacc accgtggccc gataggtgat ggaccgcgtg ccggggtgaa
      301 tggtgatctc caacgtgttg ccgacgcggg cgtccccctt cacagggtag ataaacggat
      361 tccagatcgg gtacgcagca aaattcgtca acacgagcca cacctgcgct gcggaggcct
      421 cgatctcgac gtccgtccgg aagtcggccg gggggatccc cccggggaat gaaaacactt
      481 gtgcgtgggc gacccccggc ggagcgccac cggggcccgt cacgatcacc gcgggcgcga
      541 ggagcacgcc tgcgatagcg gcaccgatga tgaagcggct cacgtcgagc gggcgcggca
      601 gaagcgccgc ggtgcaacgg cagcttgatg atccagtcgc ctcaacatcg tccaccgcac
      661 agtacgcgcg aatgatcgca acatccttca ggaggcggga cccgctggag cgccacgcgg
      721 tccgtgcgcg atccctggcc gagagtgggc cgaagtgggc atatagatgg ggtgatggtc
      781 acctgccccg ggcggcgtcg gtctcgcgcg ccgcgcctcg caggggcggt tgcgctcctg
      841 ctcgcgctgg cgctgggccg cccagcgccc gcgctgggtg aagatgccaa tcttttcgac
      901 agcgtcttcg agttgttcac ctacacccgg cagggtcatg ggatcgcgag gggcacggcg
      961 ttcttcattg accccagcgg cttggcgctc accaacagtc acgtcgtcca ccgcgtgcaa
     1021 caggatcccg agcactacgt gctgctggcg gtcgtcggca aggagttcta cggcgtggag
     1081 atcctctgcg ccagcccgct cgagctcgat cccctggagg cggccaaggg ccccgtgtcc
     1141 cgcgatatcg ccaagatccg gctggtggcc ccggacgccg agttttccct gtggggcatc
     1201 ctgcccggcg ccggcgcctc ggtcctcgcg agggcgcacc agggacccct cccgatcttt
     1261 ccggtgctga cgttgggcga cggcccgcag gagcgcggcc agatccgggt gattgggttc
     1321 ggccgggcct cgcaggcgac ggagaagacg gtcgccaccg gcacggtcac cctgaccgcg
     1381 ctcgcaaagg acggcacgcc tgtctttgag atccagtcca ctgcccggcc ggaacggggc
     1441 agcagcggct caccggtggt cgacgaccag aaccgtgtgg tcggcatgta cacctggaac
     1501 gatacccaca acgccgagtc cggcatcgcg atcagcagca ccgccctcgc tcacgcctgt
     1561 ccgtagcttc gcgctgactg gctcggtgcg ccggccctga gaggagagtg cgacgggggc
     1621 cgcgaaaccg ggggcgcgat gaccgccgcg atcgaccttc cagaggaatt caacgtctcg
     1681 accgcgtttt tggaccgaaa tctggcagag gggcggggca gccgcgtcgc catctactcc
     1741 ggcgacgagc ggatcacgta ccaggccctc gcgagccagg tcaaccgcgc ggggaacgct
     1801 ttgcgggcgc tcggcgtgcg cccggaagag cgggtcttgc tgctgatgtt ggacaccccg
     1861 gaggctgcct acctgttctg gggggcgatc cggatcgggg cggtggcggt ccccatgaac
     1921 accgccctca ggccgcccga ctacgcgtac atgctgcacg acagccgggc cgcggtgctg
     1981 gtggtgagcg aggaactgct ccccgccgtc gaacccgcgc tcggcgaccg gggccccctc
     2041 cgccatatca tcgtggccgg cggcgggacg ccggggtcgg gccgccatgc gctgcgcgac
     2101 ctcttgggcg ccgccgaccc ccgcctcgat cccgcgcgga cccacaggga cgaaccggcg
     2161 ttctggctgt ggagcagcgg tagcacgggc gcccccaagg gcacggtcca cctgcaccac
     2221 gacatggtgt gggccgccga gctgtacgcg cgcaccgtgc tggagatgcg cgccgacgac
     2281 gtctgctttt cggtcgccaa gctcttcttt gcctacggat tggggaacgc cctctacttc
     2341 ccgttccggg tgggcgccgc cacggtgctc tacccgggac ggttcgagcc ccggctgtac
     2401 ttcgatttga ttcgccggta ccggccgacc ttgttcttcg gggtgcccac cgcctacgcg
     2461 gcgatgctgg cgcaggaggg gccgaccgac cttggccccg tgcgcatgtg cgtgtctgcg
     2521 ggcgagcccc tgccggcggc gctgtttagc cggtggaagg agcggtttgg cgtcgagatc
     2581 ctcgatggga tcgggtcaac cgaggcgctg cacatctaca tcagcaaccg tcccggccgg
     2641 acccgggcgg ggagcagcgg cgagcccgtt ccggggtacg cggtccggat cgtcgacgag
     2701 accgggaggg acctgcccgc cggggagatc ggggacctgc tcgtcagcgg ggacagcatc
     2761 gcggccgggt actggaacaa gcacgagcgg accaagcagg ccttccgcgg cgagtggttc
     2821 gtgagcggcg acaagtacta ccgcgatgcc gacggctact actggtactg cggccggtcc
     2881 gacgacatgc tcaaggtcgg cgggcagtgg gtatcccccg ccgaggtgga ggcgacgctg
     2941 atccagcacc cggccgtgct cgaatgcggg gtcgtcggca aggacgacga ggacggcctg
     3001 tccaagccgt gcgcgtttgt cgtcctcaag ccggggcatg cctcaggcac cccgctggcg
     3061 aaggaactgc aggcgttcgt gcgcgacaag atcgccgcgt acaagtatcc ccggtggatc
     3121 gagttcgtgc cggaacttcc caagacggcc acgggcaaga tccagcggtt ccggctgcga
     3181 gagcggctgg gcgccgggcg caagccggcg ccggccgggt gagggggtgg cgccggtggg
     3241 agacatgatc agccttgagc gcgacggagc ggtcggccag atttcgctcc atcggccgcc
     3301 ggcgaacgcc tacaaccagc agtttgcgga ggagctggag gaggcggtgg aggccgtgcg
     3361 ggcggacgac ggcatccgcg cggtcgtggt gacgagcacc ctgccacggt ttttctccgc
     3421 gggcgcggac atcaagatgt ttcaggcttc caccttgccc gagaaggaga agttcatcct
     3481 gcgcatgcac gaggtgttgc gcaagatcga gatgacgccc aaggtgttta tcgcggcgat
     3541 caccggccac tgcctcggcg gtgggctgga gatcgccctg gcgtgcgacc tgcggttcgc
     3601 cgccgccggg aagtacggcc tggggcagcc ggagatcacg ctcgggatcc tcccgggaaa
     3661 cgggggcacc cagcggctgc cgcgccttat cgggaagagc cgggcgctcg accttatggt
     3721 cacgggccgg acggtctccc ccgacgaggc gctgtcgctc agcctcgtcg accgggtgtt
     3781 ccccgccgag gagctcgcgg ccaagacgcg ggagtacgcg gaggcgctca cccgaagcgc
     3841 ggcgatggcg gtcgggttga tcaagctcac ggtcacccgc ggcctggagc tgccgctcga
     3901 cagcgggctc gcctacgagc gggaggcgtt gttccgggtg tttgcgagcg cggacgccgt
     3961 cgagggcgtc aacgccttct tggagaagcg ccccccggtc ttcaagggaa agtaggcgcg
     4021 ggcgcgccac ctgctccccg ggggcggctc gaacgcgtcc gcgaaaaagc gcgccgccgg
     4081 tgcttcgagc gtttggtaga cgtcccggaa cacccgggcg gcgtcccacc ccggccagtc
     4141 ggatgggagc agggcctgcg gaagccccgg gtccacgaac agaaacttcc ggtattcatg
     4201 gaccagcagg atcttctccg caaagcaggc ggcatccgag ctcgtgtgtt gccggagccg
     4261 ccttgcga
//
LOCUS       DASQGH010000056         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34173, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000056 DASQGH010000000
VERSION     DASQGH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34173"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="glp"
                     /locus_tag="VEW91_01000"
     CDS             complement(<1..1472)
                     /gene="glp"
                     /locus_tag="VEW91_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYM90191.1"
                     /translation="MTVTVRLYAGLQAYGPDGTRVLVVEVPEGLTIDGLIPRLGIPPD
                     TVRRVFVNGIIREESYVLQPGDEVGVFPPIAGSGHVQEFLHIRSVADARARFVAAWSP
                     GPPRTEAIALAQAYRRVLGQDVPAPEDLPPHPRSVVDGYAVRAADTFGASEGLPAYLA
                     VTGEVLMGQAPGHAIGLGEAVRIPTGGVLPPGADAVVMVEHTETLPGGPPAAGAGSSP
                     AVPLEGVEVRRPVAPGENVIQPGEDICREDVVLRAGTVLRPPHIGLLAGLGVIQVCAA
                     VPPRVAILSTGDEVVPPERTPAAGQVRDINGPALCAAVQAEGAQPVFSGIVPDRFDAL
                     LAAMRAAKESSDLVLVSGGSSIGPRDEVSRAIDALGPPGVLVHGVAMKPGKPAVLGLC
                     GGTPVVGLPGHPTTVLVVFHVFVREIIGRLLGRAPGPAAVVQARLTRRVASAAGRTDY
                     LRVRLERRDGLLWATPILGKSGLISTMAAADGRAVVPEAV"
     gene            complement(1469..1747)
                     /locus_tag="VEW91_01005"
     CDS             complement(1469..1747)
                     /locus_tag="VEW91_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90192.1"
                     /translation="MDVYLYGALRHRFSAAAGFLPLHLEVAVGPDGTIRDVLASLAIA
                     PDEVVHLFLNGQYSAAGRRVKPGDRLGIFGRDMALLYRQYFPKVADPA"
BASE COUNT          334 a          727 c          644 g          224 t
ORIGIN      
        1 tcgaccgcct cgggaaccac cgcccggccg tccgccgccg ccatggtaga gatcaggccc
       61 gacttaccca agatcggcgt ggcccacagc aggccgtcac ggcgctccag gcggaccctc
      121 aggtagtcgg tccgaccggc cgccgaagcg acgcggcggg tgagccgggc ctgaaccacg
      181 gcagccggcc caggagcgcg ccccagaagg cggccgatga tctcgcgcac gaagacgtgg
      241 aacacgacca gaacggtggt cggatggccc ggcaggccga cgaccggtgt tccgccgcag
      301 aggcccagga cagccggctt gccgggcttc atcgcaaccc cgtgaacgag gacgccgggt
      361 ggccccagcg cgtcgatcgc gcggctcacc tcgtcgcgcg ggccgatcga actgccgccc
      421 gagaccagca cgaggtccga gctctccttc gcggcccgca tggcggcgag cagcgcgtcg
      481 aaccggtcgg gcacaatgcc ggagaagacc ggctgcgccc cttccgcctg gaccgccgca
      541 cacagcgccg gcccgttgat gtcccggacc tggcccgccg cgggggtgcg ctcgggcggc
      601 acgacctcgt cgccggtcga gaggatcgcg acgcgcggcg gaaccgccgc gcagacttga
      661 atgaccccca ggcccgccaa caggccgatg tgcggcggac gcagcaccgt acccgcccgc
      721 aagacgacgt cctcccggca aatatcctct cccggctgga tcacgttctc gccgggggca
      781 accggcctgc ggacctcgac gccctcgagg gggactgcgg gagagctacc ggcgccggcc
      841 gccggcgggc caccgggcag cgtctcggta tgctcgacca tgaccaccgc gtcggctcca
      901 gggggaagca cgccgccggt cgggatccgc accgcctctc ccaggccgat cgcatgccca
      961 ggcgcctggc ccatcagcac ctcccccgtc actgccagat aggccggtag cccctcgctc
     1021 gcgccgaacg tgtcggctgc gcggaccgcg tacccgtcca ccaccgagcg cggatgcggc
     1081 gggaggtctt cgggcgcagg cacatcctgc cccaggacgc ggcggtaggc ctgcgcgagc
     1141 gcaatcgcct cggtccgcgg aggccccggg gaccacgccg ccacaaaccg ggcccgcgca
     1201 tcggccacgc ttctgatgtg gaggaattcc tgaacgtgcc cgctacccgc gatgggcgga
     1261 aagaccccaa cctcgtcgcc aggctgcagc acgtaggact cctcccggat gatcccgttg
     1321 acaaacaccc ggcgcacggt gtcgggagga atgccgaggc gcggaatgag accatcaatg
     1381 gtgagaccct cgggcacctc gaccaccagg acgcgcgtcc catccggccc ataggcttga
     1441 aggccggcgt acaatcgcac cgtcacggtc acgccgggtc cgccaccttt gggaagtact
     1501 gccggtagag taacgccatg tcgcgcccga agatccccag ccgatccccg ggcttcacgc
     1561 gccggccggc ggccgagtac tggccgttaa gaaagagatg gacgacctcg tcaggggcga
     1621 tcgcgagcga ggcgagcacg tcgcggatcg tgccgtccgg gcccacagcc acctccaggt
     1681 gcaacggcag gaacccggcc gcggcgctga accggtgccg cagcgcgccg tacagataca
     1741 cgtccaccgc atccttaggc atagagtatg cgcatatcga ccgggaacac acccagccga
     1801 tcgcccgagc gcaccacccg ctcgaagggg acgggctggt agcggccgtt gatgaacacg
     1861 ttccccaccg ccgcccggtc gagtccaagg gccacgagca cctgatcgac tgaggcctcg
     1921 gacagatcc
//
LOCUS       DASQGH010000057         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54026, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000057 DASQGH010000000
VERSION     DASQGH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54026"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VEW91_01010"
     CDS             complement(<1..498)
                     /locus_tag="VEW91_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90193.1"
                     /translation="MKLPYHLIAALRRRMIRRYRPSRLPYAQVVGGRTWACTDLGAFP
                     RPLPRLDGLDGVVIMEDRDPNFFLSFHDYDPSADSRLNRHGPLRSWSTQEDLRDLVGG
                     LHKQRIKVAIGFWNYGGWLLHIRPRWLREHPEIRRLWLSSQLYPFVRLRREGVDYAQY
                     IAGQYQ"
     gene            685..>1697
                     /locus_tag="VEW91_01015"
     CDS             685..>1697
                     /locus_tag="VEW91_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYM90194.1"
                     /translation="MAEKEEIMRKKPSRTSRGKPRSAGGAQTPIQHVVIIVKENHTFD
                     NYFGTFPGANGVVLPAASDPLTQGPTDPPHDHRAWLKRNDPNGAVRLQYKRDDIPAYW
                     AYAQQYTLCDNYFTDVASQSEPNHLMLIAAQSPVIDNASPHRTYQPQPPYRLRSLPAV
                     LAAAGLDWRNYADPQASYFRQIAGLANDASNVPSGQFDTDVAKGYLPAVSWLYAPGGR
                     SEHPPFSGSGGPVVKPGMEWTVERVTKVAASPLWASAVIFITWDDWGGWFDHVDPPHV
                     APWTGGGPPGAGYTGSQFRYGPRVPCLVVSPYAKRGVNSTFHSHVSLVKFCLRTFSLP
                     SLGA"
BASE COUNT          298 a          547 c          555 g          297 t
ORIGIN      
        1 ctggtactgc ccggcgatgt actgcgcgta gtccacgcct tcgcgccgga gacggacgaa
       61 cggatacagc tggctgctga gccagagccg cctgatctct gggtgctcgc gaagccaccg
      121 gggtcgaatg tggagcagcc agcccccgta gttccagaag ccgattgcga ccttgatgcg
      181 ctgcttgtgg agccccccga ccagatcccg caggtcctcc tgagtggacc agctgcggag
      241 gggaccgtgg cgattcaggc ggctgtccgc gctcggatcg tagtcatgga acgacagaaa
      301 gaagttcggg tcgcggtcct ccatgatgac gacgccgtcc aggccgtcta gccgcggaag
      361 cgggcggggg aacgcgccga gatcggtgca cgcccacgtg cggcccccca cgacctgggc
      421 atagggcaag cgggaggggc gatagcggcg gatcatcctg cgccgcagcg ccgctatgag
      481 gtggtagggc agcttcacca ggcggcctgg atcttcatgg atgctggacc actgccgtaa
      541 tgatcgttgg cttcctcagg gccacgcgct gcccctcctt tggacgattc gtcggccccc
      601 tcgaggaggg gtcgctctat cgctctcgga gcggggtata gatattagga ggcaggccgt
      661 cagtcgccca gagtgccgcg tggaatggcg gagaaggagg agatcatgcg caaaaaaccg
      721 agccgaacat caagggggaa accccgttct gccggaggcg cgcagacccc catccagcat
      781 gtggtgatca tcgtcaagga aaaccacacc tttgataact acttcggtac gtttcccggc
      841 gcaaacggcg tcgtgctccc ggccgcgtcc gatcccctga cgcagggccc gactgatcca
      901 ccccacgacc acagagcgtg gctcaagcgc aacgatccga atggggctgt gcgtttgcag
      961 tataaacggg acgatatccc tgcctactgg gcctatgcac agcaatacac gctgtgcgac
     1021 aactacttca cggatgtggc cagccaatcc gagcccaacc atctgatgct gatcgcggcc
     1081 cagtccccgg tgatcgacaa tgccagccct caccgaacgt atcaacctca gcccccgtac
     1141 cggctacggt cgctgccggc cgtattggcg gcggccgggc tggactggcg caactacgcc
     1201 gatccgcagg cgtcgtactt tcggcagatc gccggcctcg caaacgatgc ctccaacgtt
     1261 ccctccgggc agttcgatac cgatgtggcg aaggggtacc tcccggcagt ttcctggctg
     1321 tacgccccgg gcggtaggag cgagcatcct ccgttcagcg gcagcggtgg gcccgtcgtc
     1381 aagccgggga tggaatggac cgttgagcgc gtgaccaagg tggccgccag cccgctgtgg
     1441 gccagcgcgg tcatcttcat tacctgggat gactggggcg gttggttcga tcacgtcgat
     1501 ccgccgcacg tggcgccgtg gacgggcggc gggcccccgg gtgcggggta caccggctcc
     1561 cagtttcgct acgggccgcg ggttccgtgc ctggtcgtga gtccctacgc caagcggggc
     1621 gtcaattcga cttttcattc ccacgtgagc ctggtgaagt tctgcctgag gaccttcagc
     1681 ctgccttcgc ttggcgc
//
LOCUS       DASQGH010000058         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42692, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000058 DASQGH010000000
VERSION     DASQGH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42692"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..725)
                     /locus_tag="VEW91_01020"
     CDS             complement(303..725)
                     /locus_tag="VEW91_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HYM90195.1"
                     /translation="MVAAGTTATFSIVAAQTPANGELNFNGADKGRLVITVPLGASVQ
                     ITLTNKGSLPHSLEIIPYSDRLPAAAVPTPALPGAMTKDPQTGIMKGQTATMQFTAGK
                     AGKYLLICGFPGHALLGMYGIFEVASSANVQPSLVIKK"
     gene            1066..>2305
                     /locus_tag="VEW91_01025"
     CDS             1066..>2305
                     /locus_tag="VEW91_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYM90196.1"
                     /translation="MRPKKIPLSRRAWGLALSMSLIFALAALGAVPRTQAAAQVLYVG
                     VNLPLTGADAQSAELIKNGVVLATEEANAKGGVAGYKIELIILDDATATAGQYDPAQA
                     ATNAKKLVANVGVVAAVGPEMSGSGKAMSPILSEGDLAIIPPSSTNPDITNPKFASQY
                     RPKGKAVYFRTCTTDAFQGPAMANYFAGKLKVRTVYVVDDAGTFGVGIADAFQKRAGE
                     KGIKVLGRDQLNPKEADYTTVLTKIKGLNPDALYYGGVSQAATKLVKQAYDVVPKMIK
                     AGADGMIDGDILEGAGFPAAEGWYASTASPHITEDPEAQPVVTRNTKRFGKQPSDYAI
                     TAYDAAVVIFDAIERVVQSKKPVNRHTVRDAIQATRLKTVQGTIQFDENGDLVNKAVS
                     IFQIKHDTLFPTNDVLHQFKF"
BASE COUNT          436 a          719 c          761 g          389 t
ORIGIN      
        1 gtcgataacc cgaccctggg ccgaggccag cggctgcagc caccactggt actttgcgac
       61 gacgaacgcg ccgaccccgg ccacgaggat caccagcacg aggatcccgg cccacatccc
      121 cgcccggcgg tgatcgtgcg catcgtgttg cgcgttcatg tcgagtccct cgacggtgcg
      181 ctaggattcc agaggcagaa acgcggctcg cccctcgcgc cgctttccac ttcggctcct
      241 gtggtgttcg tcctgagccc cgcggaaccc tttccacgcg tgctccgcct gcaggggcgc
      301 ggttacttct tgatgaccag actcggctgg acgttcgccg acgaagcgac ctcgaagatg
      361 ccgtacatgc cgagcagggc gtgcccgggg aatccgcaga tcaggaggta cttgccggct
      421 ttgccggctg tgaactgcat cgtcgcggtt tgacccttca tgatgccggt ctgcgggtcc
      481 ttcgtcatgg ctcccggaag tgctggcgtg gggacggccg ccgccggtaa ccggtccgag
      541 taggggatga tctccaggct gtggggcagg ctccctttgt tggtcagcgt gatctggacc
      601 gaggccccca gggggacggt gatcaccagg cgtcccttgt cggccccgtt gaagttcaac
      661 tccccgttcg cgggtgtctg cgccgcgacg atcgagaagg tggcggtggt ccctgcagca
      721 accacccttt ctgcgccccc gccagggagc gataacgcag ccagcagcga tgtgaggcct
      781 gcggcaactg ccaatcgacg aatcggtagc atctgtggtc tttcctcctt gttttgttgt
      841 gccgtagggt caggccgtgt cccgaaagat tataggaagc gagtcgcgat gccgcaacca
      901 cccggtctct ttcgtcgtcc gacgccgggg gcgatgccat ccggagggga acgcgcgttc
      961 ggtggcgcac caaccgagag gagattatgc ccccccgctg aacccttacc gcgaattgtc
     1021 ggtaggacta cgacgagatc catcctcaag aaagggggga agcgtatgag accaaaaaag
     1081 atcccgctct cgcgcagggc gtgggggctg gcgctgagca tgagcttgat cttcgccctg
     1141 gcagcgctgg gtgcagtgcc ccggacccag gcggcggccc aagtgctgta cgtcggggtc
     1201 aacctcccgc tgaccggcgc ggacgcccag agcgcggaac tgatcaagaa cggcgttgtg
     1261 ctggcgaccg aggaggcgaa cgccaaaggc ggggtggccg gttataaaat tgagctgatc
     1321 atcctcgatg atgccaccgc cacggcgggg cagtacgacc cggcgcaggc cgccaccaac
     1381 gccaagaagc tggtggcgaa cgtcggcgtt gtggctgcgg tcgggcccga gatgagcggc
     1441 tcggggaagg cgatgtctcc gatcctgagc gagggggact tggccatcat ccccccgagc
     1501 tccacgaacc ccgacatcac caatccgaag ttcgcgtccc agtatcgtcc caaggggaag
     1561 gcggtctact tccggacgtg cacgaccgac gccttccagg gcccggcgat ggccaactac
     1621 tttgcgggga aactcaaggt gcggacggtc tacgtggtgg atgacgcggg caccttcggg
     1681 gtgggaatcg ccgacgcgtt ccagaagcgg gcgggggaga agggcatcaa ggtcctgggc
     1741 cgggaccagc tcaaccccaa ggaggccgac tacacgaccg tcctgacgaa gatcaagggg
     1801 ttgaaccccg atgccctata ctacggaggg gtgtcgcagg ccgcaaccaa gctggtcaag
     1861 caggcctacg atgtggtccc gaagatgatc aaggctggcg cggacggcat gattgacggg
     1921 gacatcctcg aaggggccgg gttcccggca gccgagggat ggtacgcgtc gaccgcctcc
     1981 ccgcacatca cggaggatcc ggaagcccag cccgtggtga cacgaaacac caagcggttt
     2041 gggaagcagc ccagcgacta tgcgatcacg gcgtacgatg cggccgtcgt gatcttcgat
     2101 gcgattgagc gggtggtcca gagcaaaaag ccggtcaatc ggcataccgt gcgggacgcc
     2161 atccaggcca ctaggctcaa gacggtgcag gggacgatcc agttcgatga gaatggcgac
     2221 ctcgtgaata aagccgtctc gatcttccag atcaagcacg acaccctgtt ccccactaac
     2281 gatgtcctgc atcagttcaa gttcc
//
LOCUS       DASQGH010000059        11344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39873, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000059 DASQGH010000000
VERSION     DASQGH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39873"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VEW91_01030"
     CDS             complement(<1..310)
                     /locus_tag="VEW91_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90197.1"
                     /translation="MVADPNGSPDLDLAILAHLRGYPEELRRFSNLMKQAHPRGRTAA
                     EFFLSRQVAEDSFVAALSRLVRAGEDIVTVVEAADLLRVPPARVLELAAAPGFPRPLS
                     G"
     gene            complement(326..412)
                     /locus_tag="VEW91_01035"
     tRNA            complement(326..412)
                     /locus_tag="VEW91_01035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(376..378),aa:Leu,seq:cag)
     gene            complement(466..1137)
                     /locus_tag="VEW91_01040"
     CDS             complement(466..1137)
                     /locus_tag="VEW91_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYM90198.1"
                     /translation="MTPVDWITFDCYGTLIDWERGVADALGPFLPPPVDAAALAARYI
                     AIEAEVERERYRPYREILAVTAARVMEAIGRPLPAGQERVLADSLPSWRPFPEVPQAL
                     GALRAAGYRLAILSNVDRDLLAASVTHLGVQPDLTVTAEDCRSYKPTRAHWDQFRTLS
                     GTSPSRTVHVAASVFHDIVPATALGYRTVFVNRHHAPITGGPPTRVLADLRTLPQVVD
                     ELAGS"
     gene            1269..1904
                     /locus_tag="VEW91_01045"
     CDS             1269..1904
                     /locus_tag="VEW91_01045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HYM90199.1"
                     /translation="MELVDEAFTSYLEDLIPPRSPVLQEMETYARINRFPIVGPAVGQ
                     LFYLLTRLSGARRVFELGSGYGYSTAWFALGVRDNGGGEVFHVVWDNALSGAARGYLE
                     RLGLLPLIRFHVGEAVAALRGTDGRFDIIFSDIDKEGYPASLPVIKQHLSPGGLLLAD
                     NMFWRGRVVDPRATDAATESIRMFTRGVFADKDFTSTVIALRDGILLARRT"
     gene            1943..2851
                     /gene="rbsK"
                     /locus_tag="VEW91_01050"
     CDS             1943..2851
                     /gene="rbsK"
                     /locus_tag="VEW91_01050"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HYM90200.1"
                     /translation="MGSSNIDLIARVPRLPRLGETLVGRSFHLGYGGKGANQAVMAAR
                     LGARVTLVAKVGRDVFGEGAARNLKNEGIETTHVLVAADRVSGVAQILVDDSAQNVIV
                     VVPGANQDLRPAEVQRASPAILGADAVVCQLEVPVETTLEAFRIARTGGVCTILNPAP
                     AAPLPDELLDLVDICLPNEPEAEQLTGQSVATRAGVEAAARALLRRGPRAVVVTLGAA
                     GALVVDQGGAEPIPAVAVDAVDPTGAGDAFIGSLAVFLAEGVALREAAHRANAAAALS
                     VTRIGAQESFPTGDEVRAFLARLGVP"
     gene            2848..3648
                     /locus_tag="VEW91_01055"
     CDS             2848..3648
                     /locus_tag="VEW91_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYM90201.1"
                     /translation="MTVPRATRIIRLACSSESSDDLLGAGRLTLEGWLRSCAEELGLT
                     AVELEDKHIGAPTPERLGALRNTAGRHGLEIVNIAVMNNFGVADPTRLRAEEARTREW
                     MEAGQRLGSRFVRTFAGWPEGERAARWPAMIGALRSTCTEAERLGVRLVMENHNHRGF
                     VQTAADATAVFDAVRSPALGLLLDTGNYLDGLASIALTAARAWHVHAKFTRVDADGRD
                     PRVDHDAVIALLRAVGYDGWLSVEYEGAEPSRTAVPRALAYLQQALGA"
     gene            3657..5675
                     /locus_tag="VEW91_01060"
     CDS             3657..5675
                     /locus_tag="VEW91_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYM90202.1"
                     /translation="MGGRVRVGIDVGGTFTDLVAQASAGGALRMLKVPTTPDPSQAVL
                     EGLRVLLRPGEEVASLTHGTTVVTNAMLEGKGARTALVTTRGFRDVLEIGRQQRDHLY
                     RLDVPGRLPPLVPRSLRFEVTERMDHTGRVLVPLEEAGVRRIAAALRRARVDAVAITL
                     LHAYVNPSHERRIAQLLAGIVPHISLSSDVNAEYREYERTHTTCANALLMPLVDRYLA
                     DLEAGIAGARARAPLRLMQSSGGMATPAAMRRMPLAMLMSGPAGGVAAAQTVGAQAGI
                     LDAVTIDMGGTSTDVCLIRGGRVETVSERRVAGQPVRMRSLAVESIGAGGGSIARIDP
                     TGALRVGPQSAGAAPGPACYGRGGEEATVTDACVVLGYVNPEASFGGLRLDPDRAWRA
                     VESVGAPLGLAAPDAASGIVEIAGAAMTRAIRTVSVHRGYDVRRCCLIAYGGAGPIHA
                     GRLAQTLGIPRVLVPSYSSAFSAYGCLVADLRYDTVRTARLSLRDGAPATWEGVFRQI
                     EVELLTQLEREGVPAPQVMLRRSMDLRYEGQNYEIEVPAEPGEDGQAIRRRFDEIHRR
                     LYEYSTDEPVEGVNLRVAALVPAPAAPVFAEGAAAATAGGPVGMRRAHYYGRGRMPTP
                     VHPRGDLPPGREIEGPAIVEDMWSTILIYPGQRCRSDAQGHLWIEAAP"
     gene            5672..7318
                     /locus_tag="VEW91_01065"
     CDS             5672..7318
                     /locus_tag="VEW91_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYM90203.1"
                     /translation="MIDPVTLEVVRHAVYSIAEEMRAIVMRSARSPVLKEAGDLSCAL
                     TDSRGRLVAQGRDIPIHLGVMAFTVQEFLRRVPAENLRPGDVYFTNHPEVGGNHLPDV
                     KAIMPVFHGGRLVAFAINLAHWPDVGGARRGSYVAAARDRYAEGLCIPPVPLFLGGRP
                     HPAMLEVILSNVRGREEREGDILAQYAANAVAAARLGEVCDRFGADLVLRCFDRFLDE
                     SDRMMRREIARLPDGEYRGEDWLDDDGVDATPLRVAVTVRVSGEAMTFDFDGTAPEAR
                     GPLNATRFVTASAAWYAARALLGPEIPANDGCYRSLAVKVPPGSLLNPGPEAPRVGGN
                     HETSQRVVDAVLRALAPVLPDRIVAGGPGTSGLVIFSGRRADGRPFILYEVHGGGEGA
                     SERRDGTNAIRVHMSNVMNTPVEVIETEYPLRVERCELRPDSGGAGRHRGGVGLRRAY
                     RLLGERAELTTMIERMRVPPWGAFGGKDGAPFRVTLARGAKRTRVRGKENRDLVAGDL
                     VVVESSGGGGYGPPAGRASDLRALDRANGYVTRRRRRGSA"
     gene            7315..8094
                     /locus_tag="VEW91_01070"
     CDS             7315..8094
                     /locus_tag="VEW91_01070"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90204.1"
                     /translation="MRLQDRVAIVTGAAKGMGSAISAALASEGAHLVLAAREPGPLEA
                     LAATLGQPGRRCVVVPTDVADARAAEALAQRALEAFGRIDILVNAAGVIGPVETPLHQ
                     IAPADWDHVLGVNLRGVFLCCRAVVPTMIDRRYGKIINIAGTSGLRGYRFRAAYSSSK
                     WAVRGLTRTLALEVGPYGVNVNAICPGVVEGDRMTTIINQKARVRGWAPTRVVDEYVG
                     EMALRRFTRDEDIAHAVVFLASEESRQITGHELIVDGGWDV"
     gene            8110..8991
                     /locus_tag="VEW91_01075"
     CDS             8110..8991
                     /locus_tag="VEW91_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYM90205.1"
                     /translation="MPAVPFEYHAPRTLEDAIALLREGGEGAKPLAGGQSLVPLLTLR
                     LARPEVLVDLNHLEELRVVRRDGDELFIGALVRHRALERGEGVLRACPLLQEAAALIG
                     NVRVRALGTIGGSLAHADPAAELPAVAQVLDGTLVVRGPSGERRISARDFFVGALTTG
                     LGPAEVIAGVRLPVLGPGVGYAVEEFTRRAGDFAIVAAIAVVELHPTGEIARARVALA
                     GVGSTPRRLPGVEAAVAGQRPQTDILRRAAGGAAREIDPDGDAHASASYRRHLARVLT
                     ARALERAAAVAARRKTA"
     gene            8988..9464
                     /locus_tag="VEW91_01080"
     CDS             8988..9464
                     /locus_tag="VEW91_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYM90206.1"
                     /translation="MTELAIAFTLNGRPREVRVPAHRTLLEILRDDLGALEVKEGCGE
                     GVCGTCTVLLDDEPVSSCLVLAPRIAGRRITTVRGLGNGGALHPLQEAFVTHGAAQCG
                     FCTPGMILTAYAFLRDHPEPSREEIRRAIAGNLCRCTGYAKIIDAIASVAGRTDRG"
     gene            9457..>11344
                     /locus_tag="VEW91_01085"
     CDS             9457..>11344
                     /locus_tag="VEW91_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM90207.1"
                     /translation="MARPILHPSKERLRVVGRPLPRHDAWDKVQARTLYAADWQMPGM
                     LHGAVLRSPYPSGRIRRIDAAPALALPGVAAVILAGDVPHNTLWTDVPGQTSVVGPLR
                     ARIQVLADGVVRYQGEPVALVAAETPELAQRAAELIAVEYEPLPGVFDPAAALEPGAP
                     RVHEEGNLLARWRIGRGDAAPALRQAEVVVETTYRCQFVDHAYLEPESGVAWIESDGV
                     LVIRASTQVIEHYRVVAEVLGVPQNKVRVIGPYVGGGFGGKEDVTVEAFLGLLAWRTR
                     RPVRMVWSRQESLLARPKRHPFIMRYRTGAMRDGRLVAQAVDLLADSGAYAYLSALVL
                     MYATVTTAGPYRIPQVFVDARVAYTNNPPTSAMRGFGAMQMVLGYESQMDQLARRLGI
                     DPVELRARNSLRKGDRLPVGQELDTHVAVAEAARRAWQALGPRPSPSSPAHRVGRGLA
                     CNIQPYGRIVWLHDWASAWVGFEMDGSLVVRTGTPDVGGGQASSLCQIAAEVLGLDPA
                     RVSIHIADSALTPLAGTTTATRQLYMSGNAVLQACASLREQMLAVAAQMLETPVAELE
                     MREDAAVAPGGRRVALSAVMRECARLGIPRHALSTYHAPAGDPIDLERGGGRVFPDYT
                     FGAHA"
BASE COUNT         1527 a         3951 c         4260 g         1606 t
ORIGIN      
        1 ccccggacag cgggcggggg aatcctgggg cagcggccaa ctcgaggact cgcgcgggcg
       61 ggacccgaag aagatcggcg gcttcgacga ccgtgacgat gtcctccccg gcccgcacca
      121 gccgggacag cgcagcgacg aacgagtcct cggcaacctg acgcgaaagg aagaactcgg
      181 ccgcggtccg gccgcgggga tgggcctgtt tcatcaggtt gctgaaccgc cggagttcct
      241 cggggtagcc ccgcaggtgc gcgaggatgg ccaaatcgag gtcgggagat ccgttggggt
      301 cggcgaccat cgctcagcag ccgggtggtg ccgaagggga gactcgaact cccacgggct
      361 ctcgcccact acgccctgaa cgtagcgcgt ctgccaattc cgccacttcg gctcgatcgt
      421 gcacgcccgc ggccctctta gcatactgga ccctccgaaa gggtgtcaag aaccggccag
      481 ctcgtcgacc acctgaggga gcgtgcggag atcggccagc acgcgcgtcg gcgggccccc
      541 ggtgatgggt gcgtggtggc ggttgacgaa gacggtgcga tagcccaatg cggtggccgg
      601 cacaatgtcg tgaaagacac tggccgccac atggaccgtg cgcgaagggg aggtaccgct
      661 gagtgtccgg aactggtccc agtgggctcg ggtcggctta tagctgcggc agtcctcggc
      721 ggtaacggtc aggtcgggct ggacgccgag atgggtgacg gaggccgcca ggaggtcgcg
      781 gtccacgttg gacaggatcg ccaggcggta ccccgcggcg cgcagcgccc cgagcgcctg
      841 cggcacctcc gggaacggcc gccaggacgg gagggagtcc gccagcaccc gctcctgccc
      901 cgccggcaac ggccggccga tcgcctccat gacccgcgcg gccgtgacgg caaggatctc
      961 gcggtagggg cggtagcgtt cgcgctccac ctcggcttcg atcgcgatgt accgggcggc
     1021 cagcgccgcg gcgtcgaccg ggggcggaag gaagggcccg agggcatcgg cgaccccgcg
     1081 ctcccagtcg atcagcgtgc cataacagtc aaacgtgatc cagtcgaccg gggtcatcgc
     1141 gccgcgtcag cccgtgttgc tcgtgccgcc gatcgagtgc catcggccgg ctctttcggg
     1201 agcaggacga ggttctcctc gctccgtagg tatgagcagg tgcagcggga cctggtggag
     1261 ggcgggcgat ggaactcgtc gacgaggcgt ttacatccta cctggaagat ctgatccctc
     1321 cccgatctcc cgtcctccag gagatggaga cctacgcccg gatcaaccgg tttcccatcg
     1381 tcggaccggc cgtcgggcag ctgttctatc tgctcacccg cctcagcggc gctcggcggg
     1441 tcttcgaact cggctccggg tacgggtatt ccaccgcgtg gttcgccctg ggggttcgcg
     1501 acaacggcgg gggcgaggtc tttcacgtgg tgtgggacaa cgccctctcc ggggccgccc
     1561 gcgggtacct ggaacgactg gggctactgc cgctgatacg attccacgtc ggcgaggccg
     1621 tcgccgcgct ccgcgggacc gacggccgat tcgacatcat cttcagcgac atcgacaagg
     1681 aggggtatcc ggcctcgctg ccggtgatca agcagcacct cagccccggc ggcctgctcc
     1741 tcgcggacaa catgttctgg cgcgggcggg tggtcgatcc ccgggccacc gatgcggcga
     1801 cggaaagcat ccggatgttc acccgcggcg tgttcgccga caaggacttc accagcaccg
     1861 tcatcgccct gcgggatggg atcctgctgg cgcggcggac ctgatgcctc aaatggggcg
     1921 cgaccggccg aggatctgtg tggtggggtc ctccaatatc gacctgatcg cgagggtccc
     1981 gcggctgccc aggctcggcg agaccctcgt gggccgctcg ttccacctgg ggtacggcgg
     2041 caagggggcc aatcaggcgg tgatggcggc ccggctcggc gcgcgggtga cgctggtggc
     2101 gaaggtcggc cgggacgtgt ttggcgaggg cgccgcgcga aacttgaaga acgaagggat
     2161 cgagacgacc cacgtgctgg tcgcggcgga tcgggtctcc ggggtcgcgc agatcctcgt
     2221 ggacgactcg gcgcagaacg tgatcgtggt cgtcccgggg gcgaaccagg acctccggcc
     2281 ggcggaggtg cagcgggcgt ccccggccat ccttggcgcc gatgcggtgg tctgccagct
     2341 ggaggtgccg gtcgagacga cgctggaggc gttccgcatc gctcggaccg gcggcgtttg
     2401 cacgatcctc aacccggccc cggccgcgcc cctgcccgac gagctgctcg atctcgtgga
     2461 catctgcctt cccaacgagc ccgaagccga gcagttgacg ggccagtccg tcgcgacgcg
     2521 cgcgggggtt gaggcggcgg cgcgggccct cctccggcgc ggcccgcggg cggtggtcgt
     2581 gaccctcggc gccgccggcg cgctggtcgt cgatcagggc ggggccgagc ccatcccggc
     2641 ggtggccgtg gacgcggtcg accccacggg cgcgggcgac gcgttcatcg gcagcctggc
     2701 cgtgtttctc gccgaggggg tggcgctgcg cgaggcggcg caccgcgcca atgcggccgc
     2761 ggcgctctcg gtcacgcgga tcggcgccca ggaatccttc cccacggggg acgaggttcg
     2821 ggcgttcctg gcccggctgg gggtgccgtg accgtgccgc gcgcgacccg catcatccgc
     2881 ctcgcctgca gctcggagtc ctccgacgat ctgctcgggg ccgggcgcct gacgctcgag
     2941 gggtggctgc gctcttgcgc cgaagagctg ggcctcaccg ctgtggagct cgaggacaag
     3001 cacatcggcg cccccacccc ggaacggctc ggcgcgctgc ggaacaccgc ggggcgccac
     3061 ggcctggaga tcgtcaacat cgccgtgatg aacaacttcg gcgtcgccga ccccacgcgg
     3121 ctccgggccg aggaagcgcg gacccgcgag tggatggagg ccgggcagcg actcggctca
     3181 cgcttcgtcc ggacgtttgc cgggtggccc gagggcgagc gcgccgcgcg ctggccggcg
     3241 atgatcggtg cgctgcggtc gacgtgcacg gaggccgagc ggctcggggt gcggctcgtg
     3301 atggagaacc acaaccaccg cggcttcgtg cagacggccg cggacgcgac cgccgtcttc
     3361 gacgcggtgc gcagcccggc gctcgggttg ctgctcgata cgggcaacta cctggatggg
     3421 ctcgcttcga tcgcgctcac cgccgcgcgc gcctggcacg tgcacgccaa gtttacccgg
     3481 gtcgatgcgg acggtcgcga cccgcgcgtc gatcacgacg cggtcatcgc cctcctccgg
     3541 gcggtcgggt acgatggatg gctctcggtc gagtacgaag gcgcggagcc gagccgcacg
     3601 gcggtccccc gcgccctcgc atacttgcaa caggctctgg gagcgtaagc cgggcgatgg
     3661 gcgggcgggt ccgcgttggc atcgacgtgg gcggcacctt caccgatctg gtggcgcagg
     3721 cgtccgccgg cggggcgctg cggatgctga aggtcccgac gacccccgac ccctcccagg
     3781 cggtcctcga aggccttcga gtcctgctcc ggcccggcga ggaagtcgcc agcctgacgc
     3841 acgggacaac cgtggtgacc aacgcgatgc tggaggggaa gggcgcgagg acggcgctgg
     3901 tgacgacccg cggattccgc gacgtcctgg agatcgggcg ccagcagcgc gaccacctgt
     3961 accgcctcga cgtgccgggg cggctgcccc cgctcgttcc gcggtcgctc cgcttcgagg
     4021 tgaccgaacg catggatcat accgggcgcg tcctggtgcc gctcgaggag gccggggtgc
     4081 ggcggatcgc ggccgccctc agacgcgcgc gggtcgacgc ggtggccatc accctgctgc
     4141 acgcgtacgt caacccctcc cacgagcgcc ggatcgccca actcctggcg gggatcgtcc
     4201 cgcacatcag cctctcgtcg gacgtgaacg cggagtaccg cgagtacgag cgcacgcaca
     4261 ccacctgcgc caacgcgctc ctcatgccgc tcgtggaccg ctacctcgcg gatctggaag
     4321 cggggatcgc gggcgcgcgg gcgcgggcgc cgctgcgtct gatgcaatcg agcggtggga
     4381 tggcgacccc ggccgcgatg cgcaggatgc cgctcgcgat gctgatgtcg ggccccgcgg
     4441 ggggggtcgc cgcggcccag accgtggggg cgcaagccgg catcctcgac gcggtgacca
     4501 tcgatatggg cgggacgtcc acggacgtct gcctgatccg cgggggtcgc gtggagacgg
     4561 tgtcggagcg ccgggtcgcg gggcagcccg tccggatgcg gtcgctcgcc gtcgagagca
     4621 tcggtgccgg cggcggatcc atcgcgcgga tcgatcccac cggggcgttg cgcgtagggc
     4681 cgcaaagcgc cggggccgcc cccggcccgg cctgctacgg gcgcggggga gaggaggcga
     4741 ccgtcaccga cgcctgcgtc gtcctgggct acgtcaaccc ggaggcgtcg ttcgggggac
     4801 tgcgactgga ccccgaccgc gcctggcgcg ccgtcgagtc ggtgggagcg cccctggggc
     4861 tggcggcgcc cgatgcggcc tcagggatcg tcgagatcgc cggcgcggcg atgacgcgcg
     4921 cgattcgaac ggtctcggtg caccgggggt acgacgtgcg ccgttgctgc ctgattgcgt
     4981 acgggggagc cggaccgatc cacgccggcc ggctggccca gaccctgggg atccccaggg
     5041 tcctcgtgcc ttcctattcg agcgccttct ccgcgtacgg ctgcctcgtc gcggatctcc
     5101 ggtacgatac cgtgcggacg gcgcggttga gtctccgcga cggcgccccc gccacctggg
     5161 aaggggtatt ccggcagatc gaggtcgaac ttcttaccca gctcgagcgt gagggggtgc
     5221 ccgcccctca ggtgatgctg cgccgttcga tggatctccg gtacgagggc cagaactacg
     5281 agatcgaggt gccggcggag ccgggtgagg acggccaggc catccgccga aggttcgacg
     5341 agatccaccg gcgtctgtac gagtactcga cggacgagcc ggtcgaaggg gtcaacctgc
     5401 gcgtcgccgc cctcgtgccc gctccggcgg cccccgtgtt cgcggagggc gcggcggccg
     5461 cgaccgccgg cggccccgtc gggatgcgcc gggcccacta ctatggccgc ggccggatgc
     5521 cgacgcccgt ccatcccagg ggggacctcc ccccagggcg ggagatcgag ggcccggcga
     5581 ttgtggaaga catgtggtcc accatcctga tctacccagg ccagcggtgc cgcagcgacg
     5641 cccagggcca cctgtggatt gaggcggccc cgtgatcgat ccggtgacgc tggaggtggt
     5701 gcgccacgcc gtctattcga tcgccgagga gatgcgggcg atcgtgatgc ggtccgcccg
     5761 ttctcccgtc ttgaaggagg ccggcgacct ttcgtgcgcc ctcaccgact cccgggggcg
     5821 gctggtggcc caggggcgcg acatcccgat ccacctcggc gtcatggcgt tcaccgtgca
     5881 agagttcctg cgccgcgtcc ccgccgagaa cctccgcccg ggcgatgtgt acttcaccaa
     5941 tcatcccgag gtcggaggca accacctacc cgacgtcaag gcgatcatgc cggtgttcca
     6001 cggcggccgc ctggtggcgt ttgccatcaa cctcgcccac tggccggacg tcggcggcgc
     6061 ccggcgcgga agctacgtgg ccgccgcccg cgaccgatat gcggagggcc tgtgcatccc
     6121 cccggtgccg ctcttcctcg gcggacgccc gcaccccgcg atgctggagg tgatcctgag
     6181 caacgtgcgg gggcgcgagg aacgggaggg cgacatcctg gcgcagtacg ccgcgaacgc
     6241 ggtggcggcg gcgcgcctcg gggaggtctg cgatcggttc ggggccgacc tcgtcctgcg
     6301 ctgcttcgac cggttcctcg acgaatccga ccggatgatg cgccgcgaga tcgcacgcct
     6361 gccggacggg gagtaccgcg gggaggactg gctcgacgat gacggggtcg acgccacccc
     6421 gctccgggtg gccgtgacgg tccgggtgtc gggggaggcg atgacgttcg acttcgacgg
     6481 caccgccccc gaggcacggg gcccgctcaa cgccacccgg ttcgtcaccg cctcggcggc
     6541 ctggtacgcg gcccgggccc tgctcggtcc ggagattccg gcgaacgacg ggtgctaccg
     6601 gtccctcgcc gtcaaggtgc cccccggctc cctcctcaac cccgggccgg aggccccgcg
     6661 cgtcgggggg aaccacgaga cctcccagcg cgtggtcgat gccgttctcc gggccctggc
     6721 cccggtgctg cccgaccgga tcgtggccgg cggtcccggc acctcggggc tggtcatctt
     6781 cagcggccgg cgcgccgatg ggcggccgtt cattctctac gaggtccacg ggggtggcga
     6841 aggggcgagc gaacgacgcg acggcacgaa cgccatccgg gtgcacatga gcaacgtgat
     6901 gaacacgccc gtggaggtga tcgagaccga gtatccgctg cgggtcgagc gctgcgagct
     6961 ccggcccgac agcggcggcg ccggccggca ccgcggcggg gtgggcctgc gccgcgccta
     7021 ccggttgctc ggtgagcgcg ccgagctgac gaccatgatc gagcgcatgc gggtgccgcc
     7081 gtggggagcc ttcgggggga aagacggcgc accgttccgg gtgacgctgg cgcggggagc
     7141 gaagcgcacc cgggtgcgcg ggaaggagaa ccgggacctg gtggcgggcg acctcgtcgt
     7201 ggtcgagagt tccggtgggg gcggttacgg tccgcccgcc ggccgtgcgt ccgatctccg
     7261 ggcgctcgac cgggcgaacg gctacgttac gcgccgcagg aggaggggat ccgcgtgagg
     7321 ctccaggatc gggtggcgat cgtcaccggc gcggccaagg ggatggggag cgcaatctcg
     7381 gcggcgctcg catccgaggg ggcgcacctc gtcctggccg cccgcgagcc cgggccgctc
     7441 gaggcgctgg ccgcaacgct cgggcagccg ggccggcggt gcgtcgtggt gccgacggat
     7501 gtggcggatg cccgcgcggc cgaggcgctg gcacagcggg cgctcgaggc gtttggccgg
     7561 atcgacattc tcgtcaacgc ggccggcgtc atcgggcccg tcgaaacccc gctccaccag
     7621 atcgcgcccg cggactggga ccacgtgctc ggcgtgaacc tgcgcggcgt cttcctctgc
     7681 tgccgcgcgg tcgttcccac gatgatcgac cggcggtatg gcaagatcat caacatcgcc
     7741 ggcacctcgg gcctgcgggg ctaccgcttc cgcgcggcgt actcctcgtc caagtgggcc
     7801 gtgcgcgggc tcacccgcac gctcgcgctc gaggtggggc cctacggggt gaatgtgaat
     7861 gccatctgcc cgggggtggt ggaaggcgac cgcatgacca cgatcatcaa ccagaaggcg
     7921 cgggtccgcg ggtgggcgcc gacccgggtg gtcgacgagt acgtcggcga gatggcgctc
     7981 cggcggttca cccgggacga ggacatcgcc catgccgtcg tcttcctcgc ctcggaggag
     8041 agccggcaga tcaccgggca cgagttgatc gtcgacggcg ggtgggacgt ctgagcggag
     8101 gacccgagga tgccggccgt cccgtttgag taccacgccc cgcggacgct cgaggacgcc
     8161 attgcgctgc tgcgcgaggg gggcgaggga gcgaagccgc tggccggggg ccagagcctg
     8221 gtgccgctcc tgaccctcag gctcgcgcgc ccggaggtgc tggtcgacct gaaccacctc
     8281 gaagagttgc gggtcgtacg ccgggatggg gatgaattgt tcatcggcgc gctggtacgg
     8341 caccgggcgc tcgagcgcgg ggaaggggtg ctgcgggcct gcccgctcct ccaggaggcg
     8401 gcggcgttga tcgggaacgt ccgggtgcgc gcgctcggga cgatcggcgg gagcctcgcc
     8461 cacgccgatc cggcggccga actgccggcg gtcgctcagg tcctcgatgg gacgctggtc
     8521 gtcagagggc ccagcgggga gcggcgcatc tccgcgcgcg acttcttcgt cggggcgctc
     8581 acgaccgggc tcggccccgc cgaagtgatc gcaggcgtcc gcctgccggt cctgggtccc
     8641 ggcgtcgggt acgcggtcga ggagttcacc cggcgcgccg gggattttgc gatcgtcgcg
     8701 gcgatcgccg tcgtcgagct gcatccgacc ggtgagatcg cccgcgcccg cgtggcgctg
     8761 gccggtgtcg gatccacccc caggcgcctc ccgggcgtcg aagccgccgt ggccgggcag
     8821 cggccccaga ccgacatcct gcgtcgggcc gcaggcggtg cggcgcgcga gatcgacccc
     8881 gacggcgacg cgcacgcgtc cgcctcctat cgccgtcacc tggcccgcgt gctcacggcc
     8941 cgggccctcg aacgggcggc ggcggtggcc gcccggagga agacggcgtg accgagctcg
     9001 cgatcgcctt cacgctgaac ggacgcccgc gggaggtccg ggtccccgcg caccgcaccc
     9061 tgctggaaat cctgcgcgac gacctggggg ccctcgaggt gaaggagggg tgcggcgagg
     9121 gcgtgtgcgg aacctgcacg gtgcttctcg acgacgagcc cgtgagcagc tgcctggtgc
     9181 tggccccgcg gatcgcaggc cgccggatca ccacggtccg cggactgggc aacggcggtg
     9241 cgctgcatcc cctgcaggag gcctttgtga cccacggcgc cgcccagtgc gggttctgca
     9301 cgccggggat gatcctgacc gcgtacgcct tcctccggga tcaccctgag ccttcgcggg
     9361 aggagatccg ccgcgcgatc gcggggaacc tctgccggtg caccggctac gctaagatca
     9421 tcgacgccat cgccagcgtc gccgggcgga ccgatcgtgg ctagaccgat cctccatccc
     9481 tcgaaggagc gcctgcgcgt cgtcggccgg ccgctcccgc gccacgacgc atgggacaag
     9541 gtccaggcgc gcacgctgta cgcggccgac tggcagatgc ccgggatgct gcacggcgcc
     9601 gtgctgcggt cgccgtaccc gtctgggcgg atccgccgga tcgacgccgc accggccctg
     9661 gccctcccgg gagtggcggc ggtcatcctc gcgggggacg tcccccacaa cacgctgtgg
     9721 acggacgtgc cgggccagac gagcgtggtc gggcccctgc gggcacggat ccaggtgctg
     9781 gccgacggcg tggtgcggta tcagggcgag ccggtcgcgc tggtcgccgc cgagaccccg
     9841 gagctcgccc aacgggccgc cgaactgatc gcggtggagt acgagccgct tccgggggtg
     9901 ttcgacccgg ccgccgcgct cgagcccggc gcgcctcggg tgcacgagga ggggaacctc
     9961 ctggcccgct ggaggatcgg ccggggggat gccgcccccg cgctccggca ggccgaggtc
    10021 gtcgtggaga cgacctaccg gtgccagttc gtcgaccacg cgtatctcga gcccgaatcc
    10081 ggggtcgcct ggatcgagag cgacggggtg ctcgtgatcc gcgcctccac gcaggtgatc
    10141 gagcactatc gcgtggtggc cgaggtgctg ggggtgcccc agaacaaggt ccgcgtgatc
    10201 ggcccgtacg tcggcggcgg attcggagga aaggaggacg tcaccgtcga ggcgttcctc
    10261 ggcctgctgg cgtggcggac gcggcgaccg gtgcggatgg tctggtcgcg ccaggagtcg
    10321 ctgctcgcac gcccgaagcg gcatccgttc atcatgcggt accgcaccgg agcgatgcgc
    10381 gacggccgcc tggtggcgca ggcggtcgac ctgctggcag actcgggcgc gtacgcatac
    10441 ctgagtgccc tcgtcctgat gtacgcgacc gtgaccacgg ccgggccgta ccggatcccc
    10501 caggtctttg tcgatgcgcg ggtcgcctat accaacaacc cgcccacgag cgcgatgcgc
    10561 gggtttgggg cgatgcagat ggtgctcggg tatgagtcgc agatggatca gctcgcgcgc
    10621 cggctgggga tcgatccggt cgagttgcgc gcgcgcaact cgctccgcaa gggggatcgc
    10681 ctccccgtcg ggcaggagct ggacacccat gtggccgtcg ccgaggcggc gcggcgggcc
    10741 tggcaggcgc tcggtccgcg cccctccccc tcgtcccccg cgcaccgcgt ggggcgcggc
    10801 ctcgcctgca acatccagcc gtacggccgc atcgtctggc tccacgactg ggcgagcgcc
    10861 tgggtcgggt tcgagatgga tgggagcctc gtggtccgca cgggcacccc ggacgtcggc
    10921 ggcggccagg cgtcgtcgct ctgccagatc gccgccgagg tcctggggct cgaccctgcc
    10981 cgcgtgtcga ttcatatcgc ggacagcgcg ctgacgcccc tcgcgggcac aacgacggcc
    11041 acccgccagc tctacatgtc cggcaacgcg gtgctccagg cctgcgcgag cctccgcgag
    11101 cagatgctcg cggtggcggc ccagatgctg gagaccccgg tggccgagct cgagatgcgc
    11161 gaggatgcgg cggtcgcccc gggcggacgc cgggtcgccc tgtcggccgt catgcgggag
    11221 tgcgcccggc tcggcatccc tcgtcacgcg ctgtccacgt atcacgcgcc cgccggcgac
    11281 ccgatcgatc tcgagcgcgg tggcggccgg gtgtttcccg actacacctt tggcgcccac
    11341 gccg
//
LOCUS       DASQGH010000060         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5898, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000060 DASQGH010000000
VERSION     DASQGH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5898"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..910
                     /locus_tag="VEW91_01090"
     CDS             38..910
                     /locus_tag="VEW91_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM90208.1"
                     /translation="MLAALRAIARACDEADWIERAIIGIHVEGPYISPEDGPRGAHPK
                     VHVRPPDWDEVQRFQEAAGGRIRLLTLAPELPGAIDLIERLARAGIVPAIGHTNATRA
                     DIVAAASAGARLSTHLGNGSHAMIPRHHNYIWEQLAADSLWASLIVDGHHLPPSVVKV
                     FIRAKGMGRCILVSDAVSLAGEAPGAYTFLGGSVELTADRKIRLAGTEYLAGSALDLA
                     TAVGNVTAFAGVSLEDAVSMASRQPALLLGRTDLGTLTPGRAADIVLARWSPGGTTFD
                     VVQTIVGGETVYRA"
     gene            946..>1633
                     /locus_tag="VEW91_01095"
     CDS             946..>1633
                     /locus_tag="VEW91_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM90209.1"
                     /translation="MMNQRLAPGLSVVLVALLLAIGLPGIPGHAAAAMSLSLWSGYPE
                     LTPVFRAAAADYQKAHPNVTIDILTTELRDYERTLAASIPAGAAGDILQVDYTTMDRY
                     IEAGLVPKAPPDIVQFVHSRSFGAASRDLVTVGGDVYGVPYYAGIGVLYYNTAMFKEA
                     GLAGPPKTMDELMADVQKLAKRDAQGRLVRSSISFRLFGAGSGVAQKFEILMWPRGGN
                     LLVPSGAGKYK"
BASE COUNT          276 a          511 c          536 g          310 t
ORIGIN      
        1 gttctgcgcc accgtctgca cccagagcca tgagcagatg ctcgcggccc ttcgcgctat
       61 cgctcgggcc tgtgatgagg cagattggat cgagcgcgcg atcatcggca ttcacgtgga
      121 gggaccctac atctcacctg aggacggtcc acggggggct caccccaagg tccatgtccg
      181 tccccctgac tgggatgagg tgcagaggtt ccaggaggcc gcgggaggga ggatccgtct
      241 cctgacgctc gctccggagt tgcccggcgc gatcgacctc atcgagcgcc ttgcgcgcgc
      301 aggtatcgtc cctgcgattg gtcacaccaa cgcgacgcgc gccgacatcg tggcggccgc
      361 aagcgccggc gcccgcctgt ccacgcatct tggcaatggc tcgcacgcga tgattcctcg
      421 ccatcacaac tatatctggg agcagttggc ggcggacagc ctgtgggcca gcctcatcgt
      481 cgacggccat caccttcctc cgtcggtggt gaaggtgttc atccgcgcga agggtatggg
      541 ccggtgcatt ctcgtcagcg atgccgtctc gctggccggc gaggcgcccg gcgcctacac
      601 tttccttggt gggtccgtcg aactcactgc ggatcgcaag atccggctgg cgggcaccga
      661 gtaccttgcg ggctcagccc tcgacctggc gacggcggtc gggaacgtaa ccgcatttgc
      721 cggggtgtct cttgaagacg cggtcagcat ggcatctcgg caaccggcgc tactcctcgg
      781 ccggaccgat cttgggaccc tgactccggg ccgtgccgcc gatatcgtgc tggcccgctg
      841 gtcgccgggt ggcacgacat tcgacgtcgt gcagactatc gttggcgggg agaccgtcta
      901 tcgcgcgtaa cagcccaata cgctgtttcg gaggaggagg tgtgcatgat gaaccagagg
      961 ttggccccag ggttgagtgt cgtcttggtc gcattgttgc tcgccatagg gcttccgggc
     1021 attcccggcc acgctgcggc ggcgatgagc ctatcgctgt ggagtggcta cccggagctg
     1081 acgcccgtct tccgagccgc cgccgctgac tatcagaagg cccacccaaa cgtcacgatt
     1141 gatatcctga ccactgaact ccgcgattac gagcgcacac tggccgcatc cattccggcg
     1201 ggggcggcgg gagatatcct ccaggtcgat tacacaacga tggatcggta tatcgaggca
     1261 ggcctggtcc cgaaggcccc gcccgacatc gtgcagttcg tgcacagccg gagcttcggt
     1321 gcggcgtcgc gcgatcttgt cacagtgggt ggcgacgtct acggcgtgcc ctactatgca
     1381 ggcatcggtg ttctctacta caatacggcg atgttcaaag aagccgggtt ggctgggccg
     1441 cccaagacca tggacgaact gatggcggac gttcagaaac tcgcaaagag ggacgcccag
     1501 ggtcggttgg tcagaagcag catcagcttc cgactgtttg gagccggcag tggtgttgcc
     1561 cagaaattcg agattctcat gtggccgcgg gggggcaatc ttctcgttcc gagcggcgcc
     1621 gggaagtaca agg
//
LOCUS       DASQGH010000061         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37092, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000061 DASQGH010000000
VERSION     DASQGH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37092"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VEW91_01100"
     CDS             <1..775
                     /locus_tag="VEW91_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYM90210.1"
                     /translation="PNIHHAQLVLEALDRRLHVFSEKPMATSLADARRIVERVKASDR
                     VYQMGFNRRWSPAYRYLKDEIHRGFVPFSANVKINDGDMLTPSWYTNVAISGGFMYDT
                     AVHLVDMVAWLIGPIETVAAIGRRSCYPDYDDIAMLLRCRGDRPVAFTTCGHASWAAP
                     QERVELYGDHALLVSEDLDRVRCTTRQTPHAEWQKLPSADQLSLWGYVEEDRAFIAAC
                     LGEAAPPVTVKDAFHSIAVLEAAYTSVARGGVPITVPQE"
     gene            871..2433
                     /locus_tag="VEW91_01105"
     CDS             871..2433
                     /locus_tag="VEW91_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90211.1"
                     /translation="MTRKGDCSAYLTRRRFLTLAAAGLGAPLFDVGAFAQLAAAAEKR
                     GGTLSVGINTDLVSLDPNDIVFANVPMFFQVYNYLVKFTETLQPRSDLAETWELAKDG
                     MSVVFRLRAGIRTHSGGNFNAEALIANFRRVKDRTTGGGLFSRLEDWTAAEPLDGRTV
                     RFRFSRPHPDYLAQVARWGMVDPAAFPTVKNHGGGTGPFKVQEWLPGDHVTFVKFGEY
                     WQKGIPLLDRVICRVFTDPQAIENAFQAGTIDVAHTIPNKDVERFRRAGFQIMPAPVP
                     NEYYILTVNTRKPGLDNIKVRQAIQLLVDREAIVKTVLGGVGGPTVQMVTPGSPGYDR
                     KLDAEYGYNPSKAKDLLAQGGAATGLKLNFLTSTSTPELPQIAQIIQADLRKAGIDSS
                     INIQEPSQYFPVYFAGNFDLNLSFLTLATLDPTDFTISSAFRTNATNPSWLQTGLPRG
                     YLDAVQSLNGSFNMRERWTALRSAIRYILDQAWAIPIALRLPTYGLGKGVEGFSVDPQ
                     MLIQLRAVWLNR"
BASE COUNT          454 a          806 c          797 g          437 t
ORIGIN      
        1 gccgaatatc catcatgccc aactggttct ggaggcgctc gatcgtcggc tccacgtctt
       61 ttcggagaag ccgatggcga catcgctggc cgacgcacgg cgaatcgtcg agcgtgtcaa
      121 ggccagcgat cgcgtgtacc agatgggatt caatcgccgt tggtccccgg cataccgata
      181 cctcaaggat gagatccatc gaggcttcgt cccgttctct gccaacgtca agatcaatga
      241 cggagacatg ctgaccccga gctggtacac aaacgtcgcg atctccgggg ggttcatgta
      301 cgataccgcg gtgcacctcg tggacatggt ggcctggctc atcggcccca tcgagaccgt
      361 cgctgcgata ggccgccgca gctgctaccc cgattacgac gatatcgcca tgctgctgcg
      421 ctgccggggc gaccggccgg tggcgttcac gacctgcggc catgcctcct gggcggcccc
      481 ccaagagcgg gtcgagctct acggagatca cgcgctgctg gtgtccgagg atctcgaccg
      541 cgtccggtgc accacgaggc agacgccgca tgcggagtgg cagaagcttc cctccgcgga
      601 ccagctctcg ctgtggggct acgtggagga ggatcgggcc ttcatcgccg cctgcctcgg
      661 ggaagccgcg ccgccggtga ctgtgaagga cgcgtttcac agtatcgccg tgctcgaggc
      721 cgcctacacg agcgtcgccc gcgggggcgt cccgatcacc gtcccgcagg agtgacggag
      781 ccgcggcatg gcgtccgaga gccctgagcg tgcacccgag gtgaggcgct ctagactatc
      841 gaacgaaccg atccgagtgg gggtgcggag atgacccgta aaggggactg cagcgcgtat
      901 ctgacgcggc gccgcttctt gacgctcgcg gcagcggggc tcggggctcc gctgttcgac
      961 gtaggggcgt tcgcacaact cgcggctgcc gcggagaagc gtgggggaac gctctctgtc
     1021 ggcatcaaca ccgatctcgt gtcgctcgat cccaacgaca tcgtcttcgc caacgtgccg
     1081 atgttcttcc aggtgtacaa ttatctcgtc aagtttaccg agaccctgca gccgcgctcg
     1141 gatctggcgg agacgtggga gctggccaag gacgggatga gtgtggtgtt ccggctgcgg
     1201 gccgggatcc gcacccactc cggcggcaac ttcaacgccg aggcgctgat cgccaacttc
     1261 cgccgggtca aggatcggac aacgggcggc ggcctcttca gccggctgga ggattggacg
     1321 gccgcggagc cgctggatgg ccgaacggtc cggtttcgtt tctcccgccc gcacccggat
     1381 tacctcgcgc aggtggcccg gtgggggatg gtcgatccgg ccgcgtttcc cacggtcaag
     1441 aatcacggcg gcggaactgg gccgttcaag gttcaggagt ggctgccagg cgatcacgtc
     1501 accttcgtga aattcggcga gtactggcag aaggggatcc cgctgctcga ccgggtcatc
     1561 tgccgcgtgt tcaccgatcc ccaggccatc gagaacgcct tccaggccgg cacgatcgac
     1621 gtcgcacaca cgatccctaa caaggacgtc gaacggttcc ggcgcgcggg gtttcagatc
     1681 atgccggcgc cggtcccaaa cgaatattac attctgacgg tcaacacccg taagccgggc
     1741 ctcgacaata tcaaggtccg ccaggcgatc cagctcctcg tcgatcgaga ggcgatcgtt
     1801 aaaacggtgc tgggcggggt cggaggcccg acggtccaga tggtcacccc cggatcgccc
     1861 gggtacgacc gcaaactcga cgccgagtac ggatacaatc cgagcaaagc caaagatctg
     1921 ctcgcccagg gcggagcggc gacggggctg aagctcaact tcctcacgtc cacgagcacg
     1981 ccggagcttc cgcaaatagc gcagatcatc caggccgacc tcagaaaggc agggatcgac
     2041 agctcgatca acatccagga gccgtcccaa tacttcccgg tctactttgc cgggaacttc
     2101 gacctcaatc tgtcgttcct gacgctcgct accctcgacc ccaccgactt tacgatcagc
     2161 tcggccttcc ggacgaatgc cacgaacccg tcctggctcc agacggggct gccgaggggc
     2221 tacttggacg cggtgcaaag cctgaacggc tcgttcaata tgcgcgagcg ctggaccgcg
     2281 ctgcggtcgg cgatccgcta catcctcgat caggcctggg ctattccgat tgcgcttcgt
     2341 ctcccgacgt acggcctggg caaaggggtg gaaggcttca gcgtggaccc gcagatgctc
     2401 attcagcttc gggccgtttg gttgaaccga tagtatcatg gttcagggct gctatgagct
     2461 aatgcgaccc caggaaatcg tcgcggcccg cgag
//
LOCUS       DASQGH010000062         5385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39881, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000062 DASQGH010000000
VERSION     DASQGH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39881"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VEW91_01110"
     CDS             <1..1331
                     /locus_tag="VEW91_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYM90212.1"
                     /translation="LGRAAMDGTPSGSPRWPLSSRRDAETTAGGAPPGIPAWSRPGTL
                     AGNVTLFGRRLRRRGLLIGPSETSDALRALAEVDLTDREVVRLALRTVLCSRAEDLPV
                     FDDEFARFWVGPALTSGPSQAAGDEEGPPGPGPEPRSERRRELAVAEWNDSREAAEER
                     EVPAYSPVEGRTHKDFSAFAADELQAITELIVMIARLIATRLSRRTRAARRGSLIDLR
                     RTMRRSLRYGGEVLDLVWRRRKIRKAKLVLLCDISGSMDIYSRFLVQFIYALQGALSH
                     VESFVFSTSLTRVTDALGRRNIHEALQEAARQVPDWSGGTKIGASLRQFNDRYGRRLV
                     DARTIVVIVSDGWDTGDLEVLEHAMRELHARAGRVIWLNPLLGSPGYEPVTQGMSTAL
                     PYVDVFASAHSLHGLRGLERYLQNPRGAGLRGGGPRPARTAVVRPRGDTR"
     gene            1328..2455
                     /locus_tag="VEW91_01115"
     CDS             1328..2455
                     /locus_tag="VEW91_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HYM90213.1"
                     /translation="MRELRDILKALADLRARGEPAALATITRVKGSTYRREGARLLIR
                     QDATTVGSISGGCLEGDVAEAAGEVMRTGHPRMLTYDLTADDDAVWGLGLGCNGVIDV
                     FVEPARDEAGLDLAALLQDAIDHRRSAALATVIASPPGAVRVGSRVFVPEDGQVEGSL
                     GDAALDAKAVAVARSQIAKERSAVVPLDAPRGVELFVEVLTPPIPLLVCGAGHDALPL
                     VRLAHELGWWVTVADSRPAFATRERFPEADEVFLAEDRGVPGVARIDRHTFVVVMTHN
                     FLHDLELLRGLLTTPARYIGLLGPRARTDKLLAELAKQGAVLDAVQRARLYGPVGLDT
                     GADSPEEIALSIVAEILAVHNGRPAASLRDRRGPIHATVTS"
     gene            2490..3101
                     /locus_tag="VEW91_01120"
     CDS             2490..3101
                     /locus_tag="VEW91_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYM90214.1"
                     /translation="MISAIVLAAGRSERMGTPKLLLRLAGRSLLQHVMDNVRGSRCDE
                     IIVVLGEAAEQVGAEAQGDRVRLVINPRYREGMGTSLAAGIAALAPQCEAAVVLLGDQ
                     PCVTAGMIDTLIDAYRRSGKPLVASRYGPVVGAPTLIGRALFPDARRLAGDQGGRSLI
                     QQHPDQVEEVLLPPSAAVDVDTPEELARLRAALEKGARPSSAS"
     gene            complement(3383..4825)
                     /locus_tag="VEW91_01125"
     CDS             complement(3383..4825)
                     /locus_tag="VEW91_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HYM90215.1"
                     /translation="MRAEKILLFVFLVLPATFGYILLASSPGFAGTCPTATGTTPYFT
                     IGFSCVIGDKLFSDFGGFTALQTGSSFFSNLTVVPDATPFPGFRFLSGQTIPANLSGQ
                     AGINYTVSDVHGIATIQDVSSALFGSVFSGTGSISSTVTASEPGGIIAGTTSTSVDSS
                     GGLIFSEANFLPVSKLFATTFLNMNGGSDGATLYGMSTHFSSFSGLGAPGLPEDLLSL
                     KTKACDFATNLPNRLSGTFGLTALAFAPVVPPPAKGVFALVNAYNARLWFEAGKQCID
                     PFDPLYTSPVQLKIPDLSGLLAQVPPDARVTLGHGTVAAADAEAANTSLNRFLSAQSV
                     GDVAAQALQEQATIDFVRFRDTELDKYQAGLRQLAGEFNVSVQPADLQEALALFKAQG
                     AGALPPEELAVLEAFNMPPADALPDLTNVTVSDLPASAAEAFMTSADAIQKWEAPSPV
                     PEPPGAVLMLIGLLALALTRKRRRLCRRIA"
BASE COUNT          953 a         1735 c         1807 g          890 t
ORIGIN      
        1 gattgggcag agcggcgatg gatggaactc cgagcggatc gcctcgctgg ccgctcagct
       61 cccgaaggga cgctgagacc acggcgggcg gcgcgccgcc gggcatcccg gcctggagcc
      121 ggcccgggac cctggcgggg aacgtcacgc tgtttggccg ccgcctccgg cgccgggggc
      181 ttctcatcgg accgtcggag acgagcgatg ctctgcgcgc cctggccgag gtggacctga
      241 ccgaccgcga ggtagtccgt ctggcgctgc gaacggtgct gtgctcccgg gcagaggatc
      301 tccccgtgtt tgacgacgag ttcgcgcggt tttgggtcgg ccccgcgctc acgagcgggc
      361 cgtcccaggc tgcgggcgat gaggagggtc ccccggggcc cggcccggag ccccggagcg
      421 agcgccggcg cgaactggcc gtcgccgagt ggaacgacag ccgggaggcg gccgaggagc
      481 gcgaggtgcc cgcctacagc ccggtcgaag ggcgcacgca caaggatttc agtgccttcg
      541 ctgctgacga gctgcaggcg atcaccgagt tgatcgtgat gatcgcccgc ctcatcgcga
      601 cccggctctc gcgccggacc cgggcggcca ggcgcggctc gctcatcgac ctccggcgaa
      661 cgatgcgtcg cagcctccgt tacggcgggg aggtgctcga cctggtctgg cgccggcgga
      721 agatccggaa ggccaagctg gtgctgctct gcgacatcag cggatccatg gacatctaca
      781 gccgcttcct ggtccagttc atctacgcgc tgcagggcgc cctgagccac gtggaatcgt
      841 ttgtcttcag cacctcgctg acccgggtca ccgatgcgct cggccgccgc aacattcacg
      901 aggcgctcca ggaggccgcg cggcaggtgc cggactggtc cgggggcacg aagatcggcg
      961 caagcctccg gcagtttaac gaccggtacg gccgccggct cgtcgacgcc cggacgatcg
     1021 tcgtgatcgt gagcgacggc tgggacacgg gcgacctcga ggtgctcgag catgcgatgc
     1081 gggagcttca tgcccgcgcc ggccgggtga tctggctcaa ccccctgctc gggagccccg
     1141 ggtacgagcc ggtgacccag ggcatgagca ccgcgctgcc gtacgtggat gtgttcgcct
     1201 ccgcgcacag cctgcacggc ctgcgggggt tggaacgcta cctccagaac ccccgcggag
     1261 ccgggctgcg gggtggggga ccgcgcccgg cgcgcaccgc cgtcgttcga ccgcggggag
     1321 acacgcgatg agagagctcc gagatatcct caaagccctc gcagacctgc gcgcgcgcgg
     1381 ggagccggca gccctggcga cgatcacccg ggtcaagggc tcgacctacc gccgggaagg
     1441 cgcccggctc ttgatccggc aggatgcgac gacggtcggg tcgatcagcg gcggctgcct
     1501 ggaaggcgat gtcgcggaag ccgctggcga ggtgatgcgg accgggcatc cccggatgct
     1561 gacctacgat ctgaccgccg atgatgacgc ggtctggggg ctggggctgg ggtgcaacgg
     1621 ggtgatcgat gtgtttgtag agccggcgcg agatgaggcc gggctggacc tcgcggcgct
     1681 gctccaggac gcgatcgacc accggcgctc cgcggccctc gcgacggtca tcgcctcgcc
     1741 gccgggcgct gtccgggtgg gatcgcgggt gtttgtgccg gaggacggcc aggtcgaagg
     1801 ctcgctcggc gatgcggccc tggacgcgaa ggcagtggcg gtcgcgcgga gccagatcgc
     1861 caaggaacga agcgccgtcg tcccgctgga tgcgccgcgc ggggtcgaac tgttcgtcga
     1921 ggtcctcacg ccccccatcc cattgctcgt ctgcggcgcg ggacacgatg ccctcccgct
     1981 cgtccgcctg gcgcacgaac tgggctggtg ggtgacggtc gccgatagcc ggccggcgtt
     2041 cgcgacgcgc gagcggtttc ccgaggccga tgaggtgttc ctcgccgagg atcgcggggt
     2101 gcccggcgtg gcgcggatcg accgccatac gttcgtcgtc gtgatgaccc acaacttcct
     2161 ccacgacctg gagctcctcc gcgggctgct cacgaccccg gcacggtaca tcggcctcct
     2221 gggcccgcgt gcccgcaccg acaagctgct ggccgaactc gccaagcagg gagcggtcct
     2281 cgacgccgtc cagcgcgcgc ggctctacgg gcccgtcggg ttggacaccg gggccgactc
     2341 ccccgaggag atcgccctca gcatcgtggc cgagatcctg gcggtgcaca acggccgccc
     2401 ggcggcatcc ctccgcgatc ggcgggggcc gatccacgcg accgttacct cctgagcccc
     2461 acccggcgta gccgcgcccg gaccgcgcga tgatctccgc catcgtcctc gccgccggcc
     2521 gctccgagcg gatggggacg ccgaagctcc tgctgcgcct cgccggccgg agcctgctgc
     2581 agcacgtgat ggacaacgtc cgtggatcgc gctgcgacga gatcatcgtc gttctcgggg
     2641 aggcggcgga gcaagttggc gccgaggccc aaggcgaccg cgtgcggctg gtgatcaacc
     2701 cccgctaccg ggaaggcatg gggacgtcgc tcgcggcggg gatcgccgcg ctcgccccgc
     2761 agtgcgaagc ggccgtcgtc ctgctcgggg atcagccgtg cgtgaccgcc gggatgatcg
     2821 acacgctcat cgacgcgtac cggagatccg gaaagccgct cgtggcctcc cggtacggac
     2881 cggtcgtggg agcccccacc ctgatcggcc gcgcgctctt ccctgacgcc cggcgcctcg
     2941 ccggggacca gggcgggcgg tcgctgatcc agcagcaccc ggaccaggtc gaggaagtgc
     3001 tcctcccgcc gtcggcggcg gtggacgtcg atacccccga agagttggcg cgcctccgcg
     3061 cggcgctcga gaagggcgca cgaccgtcga gcgcctcttg agcatagatt gtgtcgatcg
     3121 atgctcgatt acggccacgc aaacagcaac tcggacgagg catagcacaa tgcggcaatc
     3181 gtcagcagtc gcaccacgag gtcggcccaa aaatcagccc gccatcgccg cgggagcaac
     3241 tcggcagcga tcgcaggccc aatggccacc gctgctctgt catggggtgg cagttgcagc
     3301 ataaagggga tcgaatcctt caggccaaac cacagcccga acacgacaaa cgcagtaacg
     3361 caaaagaggc gccatagcct ctttaggcga ttcgtctgca tagtctgcga cgctttctcg
     3421 tgagcgcgag cgcaagcagg ccgatgagca tgagcacagc cccgggcggc tctggcacgg
     3481 ggctgggagc ctcccacttc tggattgcat ccgcgcttgt catgaatgcc tccgccgcag
     3541 acgctgggag gtcggaaacc gtaacattcg tgagatccgg gagcgcgtca gcaggcggca
     3601 tgttgaatgc ctccagcacg gctaactctt ctggtgggag ggcccctgcg ccctgcgcct
     3661 tgaacagggc gagggcctcc tggaggtctg ctggctgaac cgatacgttg aattctcctg
     3721 caagttgccg caggcctgcc tgatacttgt cgagctcggt gtccctgaac cgaacaaagt
     3781 cgatagtggc ctgctcttga agtgcctgcg ctgctacatc acccacgctc tgcgccgaca
     3841 agaagcgatt cagcgaagtg ttggcggcct cggcgtcggc cgcagcgacc gttccgtgac
     3901 cgagggtaac ccgcgcgtct ggtgggactt gagcgagcag gccggagagg tccggtatct
     3961 tcagttggac aggagaggtg tacagcgggt caaacggatc tatgcactgc ttacccgcct
     4021 cgaaccacaa tctggcgttg taggcgttaa ccagggcaaa cacccccttc gcaggcggtg
     4081 gcacaaccgg ggcgaaggca agagcggtga ggccgaacgt cccagaaaga cgattaggca
     4141 ggtttgtggc gaaatcgcac gctttggtct taaggctcag cagatcttct ggtaggccag
     4201 gcgccccgag tccggagaaa ctagagaaat gagtcgacat cccgtagagg gtcgcaccat
     4261 cggagccgcc attcatattt agaaatgtgg ttgcaaatag cttcgacact ggcaggaagt
     4321 tagcttcact aaagatcaac cctccgctac tgtccacaga cgtcgaggta gtgcctgcta
     4381 tgatgccacc aggctcagag gcagtgacag tgctgcttat ggatcccgtt cctgaaaaga
     4441 cactcccaaa cagggctgaa ctgacgtcct ggattgttgc aattccatgc acatcactca
     4501 cggtgtaatt gatgccggct tgtccactga ggttggcggg aattgtctgt ccggacagga
     4561 acctaaaccc tgggaaaggc gtggcatcag gaacgaccgt gagattcgag aaaaagctag
     4621 agcctgtttg aagggcggtg aagcccccaa aatcgctgaa cagtttgtca ccaattacgc
     4681 acgaaaatcc tattgtgaag taaggggtag tccctgtagc cgtcgggcac gtgccggcga
     4741 atccaggcga agaagccaag agaatgtacc caaaggtggc ggggagaacc aggaacacaa
     4801 acagtaaaat cttctccgct cgcaccgtcg agccctcctg tttattccgc tggtaggtgg
     4861 cggtggaatg tgtgggaatc gtgtgactgc tggccggaag gcgcagagca ggacacgatc
     4921 aaccctttgg gtactgggcg acagccccta ctcggccagc gcgccaccag gacctcccca
     4981 ttaaccaagg aagacgccta agttttgcat cccttccctt ctaccgagct cctcgacttc
     5041 gttcttcaaa tctaaacgag tccagctgag gtttagagac aatcaattgg tgattatcat
     5101 agtatgtgac ttctcatcaa gtcccatcct tcaatcgaag gatccaatgc tcgttcgctt
     5161 gatctcacct gacctcgctg tgagcatgtt tgtggccgtc aatttgggtg tccggtcgcg
     5221 cagcgtagtg ggcattaaca aaacggatcg atcgaatcgg cgatcgattc gcctagctgc
     5281 cgcgatgact acgagacaag cctacccggc ggcgtcggga tttctttgcg tccgcagact
     5341 gctgccccgc catgcggatc tccagggggg agagccgacc gttca
//
LOCUS       DASQGH010000063         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22933, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000063 DASQGH010000000
VERSION     DASQGH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22933"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VEW91_01130"
     CDS             complement(<1..377)
                     /locus_tag="VEW91_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90216.1"
                     /translation="MRIRSTLPGFPAVRLRRMLADLPRSGYRLEVKPLRYRTGPHLQG
                     FCDYDAKTVTVQVPEPFRPFRQRIAYRAQRIRAHAAGGAPFRFRWFYRHVLFRTQTDV
                     IRFLYCHEYYHWYLREVLGRKGAA"
     gene            complement(364..906)
                     /locus_tag="VEW91_01135"
     CDS             complement(364..906)
                     /locus_tag="VEW91_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYM90217.1"
                     /translation="MIRPLVPDERAGALSVINTAARWYREFLSAEEVHEPEMTPAQYE
                     AEARRMTWFGAFLAGTLVGVMGLEYVQDAALLRHAYVLPEYQRRGIGSRLRAHLEGHV
                     QGVTRIIVGTYAGNYKARGVLEKAGYRLSPDSEAVLRSYYTIPADRLRASVTYEKWVG
                     SGVGEKGTARGAGVGEDEDP"
     gene            1505..2047
                     /locus_tag="VEW91_01140"
     CDS             1505..2047
                     /locus_tag="VEW91_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HYM90218.1"
                     /translation="MDAQVSYPDYITPDIKALIGREAQMEAWDEVERGAIRRFVQAVM
                     DPDPVYWDDGYAAKTRYRGVVAPPMYPMYAFRFPPNAADPLDAACNNPHFHGGSFMPR
                     FGLPEPPVPLQRVLNGGNDVEFLALARPGDRLRAKSTIEDIYQKNGRNGPMVFIRMKI
                     VITNQDGHVLLINRQTSIRR"
     gene            2044..>2922
                     /locus_tag="VEW91_01145"
     CDS             2044..>2922
                     /locus_tag="VEW91_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYM90219.1"
                     /translation="MKIAAFSLPGSELVRLGAVIGGGRLVDLVAAGKRADERFLDMPA
                     FLRGGEAAIVLARDLAESGHAVRAIEEVRLRAPLIRPGKMLAAGLNYEEHRAEAGAKM
                     PRPPYPEGFVKLASSIIGPGDSIIAWPDVKQLDYEGELAVVIGRTAHNVPAEDALDYV
                     AGYTAVNDVSARDWQMSERSRGRSPLMGKNFPTFCPMGPWLALRDEIPDPQALRIQLR
                     VNGEVRQSGSTAEMIFSVREMVAHWSRLRLEPGDLILTGTPAGVAMGRKPDPTPFWLK
                     PGDVVEVEVERVGILRN"
BASE COUNT          512 a          965 c          971 g          474 t
ORIGIN      
        1 gccgcgcctt tccggccgag cacctcgcgc aggtaccagt ggtagtactc gtggcagtag
       61 agaaagcgga tgacgtctgt ctgggtgcgg aagagcacat gccggtagaa ccagcgaaag
      121 cggaaaggcg ctcccccagc cgcgtgggcc ctgatccgct gcgcccggta ggcgatgcgt
      181 tgcctgaagg gtcgaaacgg ctcaggcacc tgcaccgtga cggtcttcgc atcatagtcg
      241 cagaacccct ggagatgcgg cccggtgcga tagcgcagcg gcttcacctc cagccggtac
      301 ccgctgcggg gcaagtctgc cagcatccgc ctgaggcgca cggcgggaaa tccgggcaac
      361 gtgctacgga tcctcatcct cccccacccc cgcgccgcgg gccgttccct tctctcccac
      421 accgcttccg acccactttt cgtaggtgac cgacgcccgg agccggtccg ccgggatcgt
      481 gtagtacgat cgcagcacgg cttcggagtc gggagacagc cggtatccgg ccttctccaa
      541 gacaccgcgg gccttgtagt tccccgcata ggtcccgaca atgatccgcg ttactccctg
      601 aacgtggccc tcgagatgcg cgcggagccg cgacccgatc ccccggcgct ggtactcggg
      661 caggacgtac gcatggcgta gcagagccgc gtcctgaacg tattcgagcc ccatgacgcc
      721 caccagcgtg cctgcgagaa aggccccgaa ccaggtcatc cgccgcgctt ccgcctcata
      781 ctgggccggc gtcatctcgg gctcgtgaac ctcctctgcg gagagaaact cccggtacca
      841 gcgggcggcc gtattaatga cggagagcgc cccggctcgc tcatcgggga ctagaggcct
      901 gatcatcaat gcgtgtgcgc cttcttgctt catgcgcgtc gccgtcggta cgcgtcgcgg
      961 acagattgga ttccggtacg cgccgtcaca ggggccaaca tctcaacccg cccttgagta
     1021 gtcgccctgt ggggatgcct tccggcattc cacccgcgga acccccgtct ggtgcgccta
     1081 ccccgcgctt agccgggaga cggtcgagat cctccgcgaa gcccggggcg acaaggatca
     1141 cgggaggtcg ctctccgctg ccgggctcac cctctggtgg gccccaggat tgccttgacc
     1201 tggcggtaca acgcagacgt catcacaacc cgggctcctg gccgccacag ccgggaattg
     1261 cggactcttc aatcgccggg gattcgccac ctaccgattg cacggcaaga aaccctttcg
     1321 tctggtccat tggttcaaag cgccacaggg agagaattcc gagggaggat ctgggcgcag
     1381 ggggtttgac ccattaagta ataatattat aatgcacccg gcagttcgtg gcggcgatga
     1441 acagcttcaa cttcagggcc atcatcgacg aggcgaagaa gttcaaggcc tgaggggagt
     1501 cgcgatggac gcgcaggtga gctacccgga ctacatcacg cctgacatca aggcgctgat
     1561 cggcagggag gcccagatgg aagcgtggga cgaggtcgag cgcggggcca tccgccggtt
     1621 cgtccaggcg gtgatggacc ccgacccggt gtactgggac gacgggtacg cggccaagac
     1681 gcgctacagg ggtgtggtgg cgccgccaat gtatccgatg tacgcgtttc gctttccgcc
     1741 gaacgcggcc gacccgctgg atgcggcctg caacaatccc cacttccacg gcgggtcgtt
     1801 catgccgcgc ttcggcctgc cagagccgcc cgtgcccctc cagcgggtcc tcaacggggg
     1861 gaacgatgtg gagtttctgg ccctcgcgcg ccccggggat cggctccggg ccaagagcac
     1921 gatcgaggac atctaccaga aaaacggccg gaacggcccg atggtcttta tcaggatgaa
     1981 gatcgtgatc accaaccagg acggtcacgt gctcctgatc aaccgccaga cgtcgatcag
     2041 acgatgaaga tcgcggcgtt ctcgctgccc ggctcggagc tggtacggct cggcgcggtg
     2101 attgggggtg gccgtctcgt cgaccttgtg gcggccggga agcgagccga cgaacgcttc
     2161 ctcgacatgc cggccttcct gcggggcggc gaggcggcga tcgtcctcgc gcgcgacctg
     2221 gccgagtctg gtcacgcggt ccgcgccatc gaggaagtgc ggctgcgggc tccactgatc
     2281 cggccgggca agatgctcgc ggccggcctc aactatgagg agcaccgcgc cgaggccggt
     2341 gccaagatgc cgagaccgcc ctatcccgag gggttcgtga aactggcgag ctcgattatc
     2401 ggccccggag attcaatcat cgcctggccc gacgtgaagc agctcgatta cgagggtgag
     2461 ctggcggtgg ttatcggcag aacggcccac aacgtccccg cggaggatgc gctcgactac
     2521 gtcgccggct acaccgcggt gaacgacgtc agcgcgcgtg actggcagat gagcgaacgg
     2581 agccgggggc gcagcccgct catggggaag aactttccga ccttctgccc catggggccg
     2641 tggctggcgc tgcgggacga gatccccgac ccccaggccc tgcgcatcca gttgcgggtc
     2701 aacggggagg tccgccagtc gggctcgacg gcagaaatga tcttctcggt ccgcgagatg
     2761 gtggcccact ggtcgaggct gcggctggag cccggcgacc tcatcctcac cgggacgccc
     2821 gccggcgtgg cgatggggcg gaagcccgac ccgaccccgt tctggctgaa accgggagac
     2881 gtcgtcgagg tcgaggtcga gagggtgggg atcctccgga ac
//
LOCUS       DASQGH010000064         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22940, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000064 DASQGH010000000
VERSION     DASQGH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22940"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VEW91_01150"
     CDS             <1..585
                     /locus_tag="VEW91_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYM90220.1"
                     /translation="VGRTRAAVVKLLQVPIAHYFVFNLPAMRHLVDLVGGVPITVEKR
                     MLYNDREQGLFINLQPGPQVLDGVHAEQYLRFRNDSEGDIGRIRRQQHFLRAALQAVH
                     RPAVWVRLPQLITAARADLGTDLTSEQLLGWVYEVNHLGPDAVSAQTIEGRPATLFDS
                     IMRMQLSFWIPDPDDLRAKVRWLITGEPPPPPKP"
     gene            complement(603..1343)
                     /locus_tag="VEW91_01155"
     CDS             complement(603..1343)
                     /locus_tag="VEW91_01155"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90221.1"
                     /translation="MHDVTPHTVVITGAAGGIGRAAAEKFAAGGAHVVAVDLQAPQAT
                     ADAIAGRGGRCLAVAADVAERSQVKAMISQAVDRYGGVDILVANAGIGFPDPILGISE
                     ENWDRVLAVNLKGAFLCIQATLPHMLERRRGAIVLVSSIAGRRSSLINGAHYTCSKYG
                     LIGLTRHLAAELAATGIRVNCVCPGPTETPLLWRVLTSEQRAEIARKTPLRRLADPDD
                     VAEVIAFLAGDGARHIHGAVLDVNGGLY"
BASE COUNT          240 a          544 c          520 g          245 t
ORIGIN      
        1 gttgggcgga cgcgcgccgc cgtggtgaag ctgctgcagg tgccgatcgc gcactatttc
       61 gtcttcaacc tccccgcgat gcgccacctg gtcgatctgg tcggtggcgt cccgatcacc
      121 gtggagaagc ggatgctgta caatgaccgg gaacagggcc tcttcatcaa tctccagccg
      181 ggaccgcagg tgctcgacgg ggtacacgcg gagcagtatc tccggttccg caacgactcc
      241 gagggcgaca tcggccgcat ccggcggcag cagcacttcc tgcgcgccgc cctgcaggcg
      301 gtccaccggc ccgcggtctg ggtcaggctc ccacagctca tcacggctgc gcgtgccgac
      361 ctgggcaccg atctcaccag cgagcaactc ctggggtggg tgtacgaagt taaccacctg
      421 ggccccgatg ccgtctccgc acagacgatc gagggacgcc ccgccacgct gttcgacagc
      481 atcatgcgga tgcagctgag cttctggatc cccgacccgg acgacctgcg cgcgaaggtc
      541 cggtggctca tcacgggtga acccccgccg ccgcccaagc cctaggggtc gggcttgggg
      601 ccctagtaga ggccgccgtt gacgtccagg accgcgccgt gtatgtgccg cgcgccatcc
      661 ccggcgagga acgcaatgac ctcggccaca tcgtcggggt ccgccaacct gcggaggggg
      721 gtcttgcggg cgatctcggc ccgctgctcc gatgtcaaca cccgccagag cagcggggtc
      781 tcggtcgggc ccgggcagac gcagtttacg cggatccccg tcgccgccag ctcggccgcg
      841 aggtggcggg tcaggccgat cagcccgtac ttcgagcagg tgtaatgggc gccgttgatc
      901 aggctgctgc ggcggccggc gatggatgac accagcacga tcgcgccccg ccggcgctcg
      961 agcatatgcg gcagtgttgc ctggatacag aggaaggccc ccttcaggtt aaccgccaac
     1021 acccggtccc agttttcctc cgagatccca aggatcggat cggggaaccc gatgccggcg
     1081 ttggcaacca ggatatcgac cccgccgtat cggtcgacgg cctgggagat catggccttg
     1141 acctggctac gctccgccac gtcggccgca accgcgaggc atcggccgcc gcggcccgcg
     1201 atcgcatccg cggtcgcctg gggggcctgg agatcgactg cgaccacgtg cgcgccgccg
     1261 gccgcaaact tctcggccgc tgcccgcccg atccctcccg ctgcgccggt gatcaccacg
     1321 gtgtggggcg tgacatcgtg cactccatat ccctcctctg cccacccccc ccggtggccc
     1381 ggaccagagc ttccggcggc gcgcgtgtct acgaaggggc ggcgtgcggt atgtaatagg
     1441 aatggtacag tatcgaaatg ctgggaggaa cgatcatgcg agggcggttt gttggagcgg
     1501 cactgacagc ctcgctggtc ctggcgctcg cggcgccgac gtgcgccgc
//
LOCUS       DASQGH010000065        12621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34222, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000065 DASQGH010000000
VERSION     DASQGH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34222"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1649)
                     /locus_tag="VEW91_01160"
     CDS             complement(51..1649)
                     /locus_tag="VEW91_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYM90222.1"
                     /translation="MGQQVVRWIVMVTAVIVLTTSGPAGMVGGQEAGVLRVPLGGEPP
                     TLDPYFAVDSASSPIVMMMYDTLVRIDGNGQIVPDGAQSWDVSPNGLIYTFHLRPLQF
                     HSGRRVTAADWKWSFERMSDPALKAPVGEFALPSIQGYPARQAGAAEVAGIRVIDPMT
                     LQFVLDAQRRGGFLSRLAYYAAVVLDRTIVEGGGRGWYATRDAGTGPFALKEWAHNDH
                     VVLASNPQYALGAPALSRLELPIVTQPPTQLAEYQAGQLDVIPVPLGEFPRIKADATL
                     GKQLLLSPRVQVIYLGLNPRVYAPFKDARVRRAVALAIDKETIARAVFFDLFRTAHSI
                     VPPGVPGFYESYKGLPYDPAQAQKLLAEAGVQGKLPPLTIALNPLGPVGQMAGEPVAA
                     MLKQNLGLAASLQRTEFSTFIASLNKRNVYEAFLTGWTADYLDYSDYLDVLLHSKSPI
                     DRVNYENPRFDQLIEQANAASTSGQRIALYHEAEAMAVEEAPLVPLFFTQSVLLKKPY
                     VIGFQTSPINPLGFLPPTGVTIRK"
     gene            complement(1753..2967)
                     /locus_tag="VEW91_01165"
     CDS             complement(1753..2967)
                     /locus_tag="VEW91_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM90223.1"
                     /translation="MPVYEAKVPYGPEDRRPWTVLPFPRSEYERRVAELRERLRANRL
                     AAMVAFSSNADPGHARYLANFEAGPGETCVVVPLDGSPMLTTNWLMHGEPMHTAIWTT
                     WLQDVRPAERAGFVMSPLTTVAGQVAEKLAEAGVHDGRIGVAGTAIIPYRVFAELRER
                     LAKAELADADDLLLAVRARKSPLEIEAMRRAARISGRMHQTAIEMIAPGVSERAVAAA
                     AHGVAFAEGADGLAFASAVAGGPRAGLKHCAPSDRLFQAGDMVFLDMGATYNGYCADV
                     SRSLVVGRPTHEQQRFLETGLAMFEAVLARIRPGVRVRDLIAVARGVAEREGFAADFM
                     PKGFGHGLGCSLFEQPSLRPISEAVLEAGHTFALEPMLIRTHFGTACIEETVLVTPHG
                     AEPLSGCALRAW"
     gene            3103..3933
                     /locus_tag="VEW91_01170"
     CDS             3103..3933
                     /locus_tag="VEW91_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYM90224.1"
                     /translation="MSVLPVERLTFPSAGRALIAYLHRPGAPGPWPAVVLNHGSDLTQ
                     NSLPGVAHVLATAGYLAFTAIRRGYGDSPGPTRLDEVTAPVGQPGHGDQVCARLLAES
                     DDVLAALRFLRPLPEVDRTRIAVMGSSYGGINSLLAASRDPDVRCCVSFAAAAMTWPH
                     VPEVREFLLRHADAIRCPVLLLQAENDFDLTPSLALAERLRMRGRICERHLFRPFGID
                     AMEGHRIFVYAPQIWAPVVLPFLARHLGGAHRRKGTQQRSRPAGFPRNARRPRRRPGP
                     "
     gene            3930..4748
                     /locus_tag="VEW91_01175"
     CDS             3930..4748
                     /locus_tag="VEW91_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HYM90225.1"
                     /translation="MTARARGGTSGLRGVYLEDLTWAEAEPVLRADPLVVIPIGAAAK
                     EHGPHLPLGTDHIMADYLARGLAERVEVVVMPTVTYGYYPQFSPFPGSTHIEAHTFEA
                     MVKELILSMHRHGPRRFLILNTGVSTYPVLEIVARDLERVHRLLVAVTRIGELGAQRI
                     SGLLSQPRGSHADEHETSLLLAIAPEVVRRDKIVGEIPDRPDSRGVFVPPMFHREPGL
                     GHSQTGVYGDATLGTAAKGETIARAMVEDLVAAVERLRTASVPASRPGIPWTAP"
     gene            complement(4765..6420)
                     /locus_tag="VEW91_01180"
     CDS             complement(4765..6420)
                     /locus_tag="VEW91_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90226.1"
                     /translation="MGHAAAHNPGCGQWRARLTRRALLRVLGGGAGLAAARPFLDIAN
                     AVASPAAGLLVVGASFVIKSLDPGRTIESTTEMINHATYDSLVTFEGEDLKIPRPSLA
                     TDWKVSSDGRAYTFTLRPNVRFASGNPFTSADVKWSLERVMNLKSSPLYFVDQVDAVL
                     APDPRTVVIRLKAPQPSIIPILSSPSLGVLDSKLVTENGGDAGPDAKNKDTAEPYLNV
                     HSAGSGPYVLTSYVPDQLVVLVRNPNYWRSPAPIQRIVLRNITEPATQQLQLEKGDLD
                     IATALGHDQVQALRGTAQVTIKTSPVATTFTLMMNRDPDIGGPFANPKVQQAVRYALD
                     YDGIMALAGPGAVRLAGTIPVTMPGALDPREAFKTDRARARALLKESGVGEVKGRLTF
                     MVDATVQGVPYSVLAQKIQADFFAVGITLELNGLPRVVGLQQYRDGKAQVWFSGWVAD
                     YPDPSNFLVYLPGRVAGKRARWLPEASPQAQELARMGDEAEAETNPGKRVALLQRVQR
                     TLVEIGPFIPLFQPAIPLAFRSSIHGAMFHSVWGLDFYPITKA"
     gene            6669..8276
                     /locus_tag="VEW91_01185"
     CDS             6669..8276
                     /locus_tag="VEW91_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90227.1"
                     /translation="MAKPSPTHVWLSGLVFLALAASSAWGASSAPKPGGVLNAMHRED
                     PPSLSIHEEATVSVNWGVMPCYSNLVLFNPLRGQETPATIIGELAEKWVWRDGGKSLV
                     FTLRQGVRWHDGQPFTSKDVKYTFDVVRGAPGAPPLRLSPRRVWYSQVQAIDTPDPYT
                     VIFRLKRPQPSLLMLLASGYSPVYPAHVPLAELRTRCVGTGPFKLKEYRPGELIEYVR
                     NPDYFIKGRPYLDGIRFIIIKERGIRIAALQTGRLDVTMPQEGTKLAAEQLKKAVPSM
                     VVSEVSQSINDNLLTNFKRPPFTDPRVRRALSLAIDRRAYVQAVRQGGAAVGASMLPR
                     PWGVWGLAPEDLAALPGSGDPAKQKAEARQLLAEAGFGPGRPLRFAVSTRSIVQYVDM
                     AAFVIDQLKQIGIEANLDLVESAVWFAKLTRGDYEMGANLTGVSVDDPDANFYENYVC
                     GSPRNYSQYCNPDVDRMIDEQSQMLDQVKRRRLVAEIQKRLELDGARPILGWSNDYYA
                     MWPYVKNLIPHQSVYNYARFQEVWLDR"
     gene            complement(8497..9387)
                     /locus_tag="VEW91_01190"
     CDS             complement(8497..9387)
                     /locus_tag="VEW91_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90228.1"
                     /translation="MSVGIQAGVSRARTGRFVRLGRILPRRFAQSPLGLAAVGIAAAW
                     VCLALAASRIAPYPPLAQDLMQRLAPPSPAHWLGTDPLGRDVFSRILHGARLSIPVGI
                     TAVTLAMLLGTLVGGTAGFVGGVADEAIMRTTDLMLAFPTVIMALVITAALGPGIRNA
                     VIAIMIAWWPSYARLVRGLVLSLREREYVVAARALGSSAPRVFVRHVLPGTISPVIIL
                     STLDVGHAILTFASLSFLGLGPPPQIPEWGSMIAAGRSYLTQWWISTFPGLAILRLVL
                     AFNVMGDSLRDLLDPRFRRR"
     gene            complement(9384..10403)
                     /locus_tag="VEW91_01195"
     CDS             complement(9384..10403)
                     /locus_tag="VEW91_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90229.1"
                     /translation="MNLQRYVLRRVLLLGPMLIGITLLTFILSHAVPADPVAANIGEQ
                     AASDPVVVEAFRHRWGLDQPLYKQYLVYLWHLAHGDMGTSISTRQPVTLDLKQHLPAT
                     IELATSALAISILIGIPLGILAAVNRENPIDQAARMSALVGVSIPVFWLGIVSIVIFY
                     ARLGWAPAPGRLSPSLAPPLPVSGFVLIDALLAHDTDALLDWLHHLVLPALVLSSYTL
                     GVITRIMRGSMLEVLGEDYVRTANAKGVRMWRAILHHAARNALIPIITIVGLSFGGLL
                     SGAVVTETVFNWPGLGLYAFSSATSLDFPAIMGTGLVVATIYVLVNLFVDIAYAFFDP
                     RIRVG"
     gene            complement(10457..10666)
                     /locus_tag="VEW91_01200"
     CDS             complement(10457..10666)
                     /locus_tag="VEW91_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90230.1"
                     /translation="MAPTAKRIMEQVLAVRLGERVVLIPGSCYGCEGHVRLGFGPGTP
                     AETIRLGLEHIDHFIADFKAGKVAA"
     gene            complement(10837..11541)
                     /locus_tag="VEW91_01205"
     CDS             complement(10837..11541)
                     /locus_tag="VEW91_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM90231.1"
                     /translation="MQAKEFLSRHHIAYLPRDVDNDPAAARELAELGFLTVPVVVLPG
                     QPPIPGFNARALGAAIGVGDRSTGPNVEAVLNGFDRLLAAILRVAAQLGNAGLILKMP
                     NRDRTLGELIHDVFYKALTWVPEDGGVSRRDAATQKKDAARYPDMASLLRYGEASRAT
                     LRARFSPGRGDYTRIVETPDGAMSLADAVTWLADHSAHHLRQIYWLMEHDLGVKPEDP
                     LDLATLPGITLQEKLW"
     gene            complement(11683..12519)
                     /locus_tag="VEW91_01210"
     CDS             complement(11683..12519)
                     /locus_tag="VEW91_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HYM90232.1"
                     /translation="MSVAGVDVSDYQGAVDWPAVAAAGVRFAIAKATEGADFVAETFA
                     RNWAGIQAAGLVRGAYHYFRPLVDPQIQAEHLLATVAPGPDDLPPALDVEVRDGVDAP
                     TLVQRMQRWLDAVEAAMGRKPMIYASPSFLDGLGSARGFSEYPLWIADYGRSAPIIPR
                     GWSTYTLWQYTSGGSVRGINGPVDLNIFNGSPEDLAAFVATGRPPSGGGAGDAFISEG
                     DAGPEVEEIQSRLRAKGFDPGPADGVFGPETRAAVIAFQGATNLVVDGIVGPQTLAAL
                     RG"
BASE COUNT         2086 a         4324 c         4267 g         1944 t
ORIGIN      
        1 gggcacgacg agcaccatcc ggcgcgccag gtagcgggcc acgggcgcgg ctacttccgg
       61 atcgtcaccc ccgtaggagg cagaaatccg agcgggttga tcggcgacgt ctggaacccg
      121 atgacgtacg gtttcttgag gagcaccgac tgcgtgaaga agagcggcac cagcggcgct
      181 tcctccaccg ccatcgcctc ggcttcgtgg tacagggcga tccgctggcc gctggtcgac
      241 gccgcgttcg cctgctcaat cagctgatcg aagcgggggt tctcgtagtt gacccgatcg
      301 atcggggact tgctgtgcag gaggacgtcc agatagtcgc tgtagtcgag gtaatcggcc
      361 gtccaaccgg tgagaaaggc ctcgtagaca tttcgcttgt tgagcgaggc gatgaaggtg
      421 gaaaattccg tgcgctggag gctcgcggca agacccaggt tctgtttcag catcgccgcg
      481 acaggctcgc cggccatctg ccccacgggg ccgagcgggt tcaacgcgat ggtgagcggg
      541 gggagcttcc cctgcacccc ggcttcggcg agcagcttct gggcctgggc gggatcgtac
      601 ggcaggccct tgtaggactc gtagaatccg ggcaccccgg gcgggacgat cgaatgggcc
      661 gtcctgaaca ggtcgaagaa cacggccctc gcgatcgtct ccttgtcgat cgccagagcc
      721 acggcgcgcc gcacccgggc gtccttgaag ggggcgtaga cgcgcgggtt gagccccagg
      781 tagatgacct ggacgcgcgg ggagaggagc agttgcttgc cgagcgtcgc atccgccttg
      841 atgcggggaa attcacccag cggcacaggt atcacatcga gctgacccgc ttgatactct
      901 gcgagctgcg tcggcggctg ggtgacgatc ggcaactcga ggcgggagag cgcgggggcc
      961 ccgagggcgt actggggatt gctggcgagc accacgtgat cgttgtgcgc ccactctttg
     1021 agcgcgaagg gcccggtccc ggcgtcgcgg gtcgcgtacc atcccctccc cccgccctcg
     1081 acgatggtcc gatcgaggac gaccgccgcg tagtaggcca gacggctcag gaaccccccg
     1141 cggcgctgcg cgtccaggac gaactgcagg gtcatcgggt cgatcacgcg gatgccggcg
     1201 acctctgcgg cccccgcttg ccgggccggg tacccctgga tgctggggag cgcgaactcc
     1261 ccgacgggag ccttgagcgc aggatcgctc atccgttcaa acgaccactt ccagtccgcc
     1321 gccgtgaccc gtcgcccgct gtgaaactgc aggggccgaa gatgaaaggt gtagatcaac
     1381 ccgttgggag agacgtccca gctctgcgcc ccatcgggca cgatctggcc gtttccgtcg
     1441 atgcggacca gcgtgtcata catcatcatg acgatcggcg aggacgcgct gtcgaccgcg
     1501 aagtagggat ccagcgtcgg cggttcgccg ccgagcggca cgcggaggac gccggcttcc
     1561 tgtcctccga ccatcccggc cggccccgag gtcgtgagga cgatcaccgc ggtcaccatg
     1621 acgatccatc ggacgacctg ttgacccatg cctaccctcc ggggtggagc gcactggccg
     1681 aaacgcagtt ctccgtggtc cacacgcctc ctgctgcgcg cgcgccgcgc gcgggcatcg
     1741 cgatgggggc ggctaccacg cgcgcaacgc gcagcccgac aggggctcgg cgccgtgggg
     1801 cgtgaccagg actgtctcct cgatgcacgc cgtcccgaag tgggtgcgga tcagcatcgg
     1861 ttccagggcg aacgtgtgcc ccgcttccag cacggcctcg ctgatgggcc gaagcgaggg
     1921 ctgctcgaag agcgaacagc ccagcccatg cccgaaccct ttcggcatga agtcggcggc
     1981 gaacccctcg cgctcggcga ccccccgggc caccgcgatg aggtctcgga cacgaacgcc
     2041 gggcctgatc ctggcgagga cggcctcgaa catcgccagc cccgtctcga ggaaccgctg
     2101 ctgctcatgg gtgggccgac ccacgaccag gctgcgcgac acgtctgcgc agtatccgtt
     2161 gtaggtcgcg cccatgtcga ggaagaccat gtcgccggcc tgaaacagtc ggtccgacgg
     2221 cgcgcagtgt ttcagccccg cgcgcgggcc gcccgccacg gcgctggcaa aggccagccc
     2281 atcagcgccc tcggcaaacg cgacgccgtg cgccgcggcc gccaccgcgc gctcgctcac
     2341 ccccggggcg atcatctcga tcgccgtttg atgcatccgg ccggagatgc gtgccgcgcg
     2401 gcgcatggcc tcgatctcga gcggagactt gcgcgcgcgg accgccagca ggaggtcgtc
     2461 ggcatcggcc agttcggctt tcgccagccg ctcacgaagc tcggcaaaca cgcgatacgg
     2521 aatgatcgcg gtgccggcga caccgatgcg cccgtcgtgg acgccggcct cggccagctt
     2581 ctcggccacc tggcccgcga ccgtcgtcag cggggacatg acgaaccccg cacgctccgc
     2641 ggggcggaca tcctggagcc aggtcgtcca gatcgcggtg tgcatgggtt cgccgtgcat
     2701 cagccagttc gtggtcagca tcgggctgcc gtcgagtggg accacgacgc acgtctcgcc
     2761 ggggccggcc tcgaagttcg ccaggtaccg ggcgtggccg gggtccgcat tgctggagaa
     2821 ggcgaccatc gcggcgagcc ggttggcccg gagccgctcg cgtaactcgg cgacgcggcg
     2881 ttcgtattcg gatcgaggga agggcaacac cgtccacggc cggcggtcct ccggcccgta
     2941 cggcaccttg gcctcgtaga ctggcaccct tccacctccc gtgtgcgccc gggctcgctc
     3001 ctccaacgtt ccgatccctc gccggattct cctgttgatc gcggtcgcgc ggcaggagcg
     3061 ctcccgtccg cggcaaatgg gtgggcccga ggagggtgtc tcgtgagcgt gcttcctgta
     3121 gaacggctga ccttcccgag cgccgggcgg gccctgatcg cctacctcca tcgcccgggc
     3181 gcccctgggc catggcccgc ggtggtgctc aaccacggga gcgacctcac gcagaacagc
     3241 ctgcccggcg tggcgcacgt gctcgcgacc gccggctacc tcgccttcac cgcgatccgc
     3301 cgggggtatg gcgactcgcc cggcccgaca cgcctcgacg aggtcacggc ccccgtcggc
     3361 cagccggggc acggggacca ggtctgcgcg cgccttctgg ccgaaagcga cgacgtgctg
     3421 gcggccctgc ggttcctgcg accccttcca gaggtcgacc ggacgcggat cgccgtcatg
     3481 gggtcgtcat acggcgggat caactccctc ctggccgcga gccgcgatcc cgacgtccgg
     3541 tgctgcgtct ccttcgccgc tgcggcgatg acatggcccc acgtgccgga ggtacgggag
     3601 ttcctcctcc gccacgccga cgccatccgg tgcccggtgc tgctgctgca ggcggagaac
     3661 gatttcgacc tcacgccatc cctggcgctc gccgagcgcc ttcggatgcg cggccgtatc
     3721 tgcgagcggc acctcttccg gcccttcggc attgatgcca tggaagggca ccggatcttc
     3781 gtgtacgcgc cgcagatctg ggcgcctgtg gtgctcccgt tcctggcccg gcacctcggc
     3841 ggcgctcacc ggcgcaaagg gacgcaacag aggagtcgcc ccgccggatt tcccaggaat
     3901 gctcggcggc cgcggagaag acccggccca tgacggcgcg cgcccgcggc ggcacctcgg
     3961 ggctccgcgg ggtctacctc gaggacctca cgtgggccga ggccgagccg gtgctccgcg
     4021 ccgacccgct ggtggtcatc ccgatcggcg ccgccgccaa ggagcacggt ccgcatctgc
     4081 cgctcggcac cgaccacatc atggccgact acctggcccg cggactggcg gagcgggtcg
     4141 aggtcgtcgt catgccgacg gtcacctacg ggtattaccc gcagttttcg ccgttcccgg
     4201 ggagcacgca tatcgaagcc cacacgttcg aggcgatggt caaagagctc atcctctcga
     4261 tgcaccggca cggcccccgg cggttcctca tcctcaacac cggggtgtcg acgtacccgg
     4321 tcctcgagat cgtcgcgcgc gatctcgagc gcgtgcaccg cctcctggtg gccgtgacgc
     4381 ggattgggga actcggcgcg cagaggatct ccggcctcct gagccagccc cggggcagcc
     4441 acgccgacga gcacgaaacg tccctgctgc tggcgatcgc gcccgaggtg gtgcgccggg
     4501 acaagatcgt gggagaaatt ccggaccggc cggattcccg aggcgtgttc gtcccgccga
     4561 tgttccaccg ggagcccggc ctggggcact cccaaacggg cgtgtacggc gatgccacgc
     4621 tgggcacggc cgccaagggc gaaacgatcg cccgcgcgat ggtcgaggac ctcgtcgcgg
     4681 ccgtggagcg gctccgcacc gcctcggtcc cggcgtcgcg cccgggcatt ccctggacgg
     4741 ccccctagcg gggcctgctg gcggttacgc cttcgtgatc gggtaaaagt cgaggcccca
     4801 aacgctgtgg aacatcgcgc cgtgaatgct cgatcgaaag gcgagcggaa tcgcaggctg
     4861 gaacagcggg atgaaggggc cgatctccac gagcgtccgc tggactcttt gtagaagcgc
     4921 gacccgcttg ccggggttcg tttcggcctc cgcctcatcc cccatccgcg cgagttcctg
     4981 ggcctggggg cttgcctccg gcagccatcg cgcgcgcttc cccgcgacgc ggccaggcag
     5041 gtacacgagg aagttgctcg ggtcgggata atcggccacc cacccggaga accagacctg
     5101 ggctttcccg tcacggtact gctgaagccc gacgacccgc ggcaggccgt tcagctcgag
     5161 cgtgatgccg accgcgaaga aatccgcctg gatcttctgc gccagcacgc tgtacggcac
     5221 tccctgcacg gtggcgtcga ccatgaacgt gagacgcccc ttcacctcgc ccacgccgga
     5281 ctccttgagg agcgcccgcg ccctcgcccg gtcggttttg aaggcctccc gggggtcgag
     5341 cgcgcccggc atcgtcaccg ggatggtccc ggccaggcgc acggccccgg gaccggcgag
     5401 cgccatgatc ccgtcgtagt ccaacgcgta ccgcacagcc tgctgcacct tggggttggc
     5461 gaaaggccct ccgatgtccg gatccctgtt catcatcagg gtgaacgtcg tggcgacggg
     5521 actcgtcttg atcgtcacct gggcggtccc ccgcagggcc tgcacctgat cgtgaccaag
     5581 ggccgtcgcg atgtccagat cgcccttctc cagctgcagc tgctgggtcg caggttccgt
     5641 gatattgcgc agcacgatcc gctggatggg ggccgggctg cgccagtagt tcgggtttct
     5701 gacgagcacg accaactggt ccggcacata gctggtcagg acgtacgggc cggagcccgc
     5761 ggagtggacg ttcaagtacg gttcggccgt gtccttattc ttggcatccg ggcctgcatc
     5821 cccgccattt tcggtgacga gtttgctgtc gagcacgccg aggctcgggc ttgagaggat
     5881 cgggatgatc gagggctgag gcgccttcag ccggatcacg accgtccgtg gatcaggcgc
     5941 gaggacggcg tcgacctggt ccacaaaata taacgggctc gacttgaggt tcatgacccg
     6001 ttccagcgac cacttcacgt cggcggacgt gaacgggttg ccgctcgcga accggacgtt
     6061 cggcctgagg gtgaacgtat acgcgcgccc gtcgctggag actttccagt cggtcgcaag
     6121 cgagggccgg gggattttca gatcctcacc ctcaaacgtc acgagcgagt catacgtcgc
     6181 gtgattgatc atctcggttg tcgactcgat cgtgcgacct ggatccaggc tcttgatgac
     6241 gaagctcgcg ccgacaacga gaaggcccgc agcagggctc gcgaccgcgt tcgcgatgtc
     6301 caggaagggc ctggcggcag ccagccccgc cccgccccca aggacacgga ggagcgcccg
     6361 ccgggtgagg cgagctcgcc attgcccgca cccgggattg tgggcggcgg catgccccat
     6421 gtgtatccct ccttactgct ggattgcgcg gcagtgcact gcccttcgct gatgcgcctg
     6481 ccacctcctg cgctccaggt cctcggtggc gcgggccctg ggctggttgg tggcgatgga
     6541 gaatcggggc tggaggagtt gcgcgtacga cccccgaaac tttttccggc ctcaggaatc
     6601 gtcacccctc gcgtggacta tccaatggga gccgagggga atgcgcgtcg gcgaggtgag
     6661 atggttcgat ggcgaagccg agtcccacac acgtgtggct gagcggtctg gtattccttg
     6721 cgctcgccgc atcgtcggcc tggggggcca gctctgcccc gaagcccggc ggcgtcctca
     6781 acgccatgca ccgcgaggat cctccaagcc tgagcatcca cgaagaagcc accgtctcgg
     6841 tcaactgggg ggtcatgccg tgctacagca acctggtcct cttcaacccg ctgcgcgggc
     6901 aagagacccc ggccaccatc attggcgaac tcgctgagaa atgggtgtgg cgcgacggcg
     6961 ggaagagcct cgtcttcacc ctccgccagg gcgtccgctg gcacgacggc cagcccttta
     7021 ccagcaagga cgtcaagtac accttcgatg tcgtccgcgg cgcgccgggc gccccgccgt
     7081 tgaggctgag cccccgcagg gtctggtact cgcaggttca ggctatcgac acgcccgacc
     7141 cctacacggt catcttccgg ctcaaacggc ctcagccgtc cttgttgatg ctcctcgcat
     7201 cggggtactc gccggtctac ccggcccacg tccccctcgc cgagctccgc acccggtgcg
     7261 tcggcaccgg accgttcaag ctcaaggagt accgccccgg cgagctgatc gagtacgtcc
     7321 gcaaccccga ttatttcatt aagggccgcc cctacctcga cggcatccgg ttcatcatca
     7381 tcaaggaacg cggcatccgc atcgcagccc tccagacggg gcggctcgac gtgacgatgc
     7441 cccaggaagg caccaagctc gcggcggagc agctcaagaa ggccgtcccc agcatggtgg
     7501 tgtctgaggt gagccagagc atcaatgaca atctcctgac gaacttcaag cggcctccct
     7561 ttaccgatcc ccgcgtccgg cgggcgctga gcctggccat cgaccgccgc gcgtacgtgc
     7621 aggcggtccg ccagggcggc gccgcggtcg gcgcctcgat gctgccgcgc ccctggggcg
     7681 tgtgggggct agcgcccgag gatctggccg ccctgcccgg cagcggcgac ccggcgaagc
     7741 aaaaagccga agcccgccag ctcctggccg aggcggggtt tggcccgggc cgtccgctgc
     7801 gctttgccgt cagcacccgc tcgatcgtgc agtatgtcga catggccgcc ttcgtgatcg
     7861 atcagttgaa gcagatcggg atcgaggcca acctggacct ggtcgagagc gccgtgtggt
     7921 tcgccaagct cacccgcggc gactacgaga tgggcgccaa cctgaccggc gtgagtgtgg
     7981 acgacccaga cgccaacttt tacgagaact atgtctgcgg gtccccgcgg aactactcgc
     8041 agtactgcaa tcccgatgtc gatcgcatga tcgacgagca gtcgcagatg ctcgatcagg
     8101 tgaagcggcg gcggctggtc gccgagatcc agaagcggtt ggagctggac ggcgcgcgcc
     8161 cgatcctcgg ctggagcaac gactactacg cgatgtggcc gtacgtcaag aatctcatcc
     8221 cgcaccagag cgtctacaac tacgcgcgct tccaggaggt ctggctcgac cggtagggat
     8281 taggcgccga tgcgggcctc ccccacgcgg tagcggcgcc aggcaacttc gccggcctcg
     8341 ccatggacgg caactggcgc ttgggcttgt acgtagacgc ccgggcaacc gagttttcgc
     8401 cggggagatg actgaactca ccgcagggac agcacccagg aacccctgat gtagcaatac
     8461 tgtccattcc atcgtcccgg gctggccacc ggggctctac cgcctgcgaa accgcggatc
     8521 gaggagatcc cgcaggctgt cgcccatcac gttgaacgcg aggaccaggc gcagaatggc
     8581 cagccccggg aaggtactga tccaccactg ggtcaggtag cttcgcccgg cggcaatcat
     8641 cgagccccat tccggaatct gcgggggcgg cccgagtccg aggaagctca gggacgcaaa
     8701 cgtaaggatg gcgtgaccca catcgagggt gctcaggatg atcaccggcg agattgtgcc
     8761 cgggagaacg tggcgtacaa acacgcgcgg ggcggaggag ccgagcgccc gtgcagcgac
     8821 cacgtattcg cgttctcgca gggacaggac cagtccccgc acgaggcgcg cgtaggaggg
     8881 ccaccacgca atcatgatgg cgatgacggc gtttcggatg cccgggccga gcgcggccgt
     8941 gatcaccagg gccatgatga cggtggggaa cgccagcatg agatcggtcg tccgcatgat
     9001 cgcctcgtca gccacaccac cgacgaagcc ggccgtcccg ccgaccagcg tccccagcag
     9061 catcgcgagc gtcacggcgg tgatcccgac cggaatcgac aggcgcgcgc cgtgcaggat
     9121 gcggctgaag acgtcacggc cgagcgggtc ggtccccagc cagtgcgccg ggctgggcgg
     9181 cgccagccgc tgcatgagat cctgcgcaag cggcgggtag ggcgcgatgc gcgaggccgc
     9241 gagcgcgagg cagacccacg ccgcggcaat ccccaccgcg gccaacccca ggggcgactg
     9301 cgcgaaccgg cggggaagga ttctcccgag gcggacgaac cgtccggtgc gtgcgcgtga
     9361 gacgccggcc tgaatcccca cgctcaccct acccggatcc gcggatcgaa gaacgcatag
     9421 gcgatgtcca cgaacagatt gaccaggacg tagatcgtcg cgaccaccag gcccgttccc
     9481 atgatggccg gaaaatccag cgaggtcgcg ctgctgaagg cgtagaggcc cagcccgggc
     9541 cagttgaaca cggtctccgt cacgacggcg cccgacagca ggccaccgaa gctcagtccc
     9601 acgatcgtga tgatagggat gagcgcgttg cgggcggcgt ggtgaaggat cgccctccac
     9661 atccgcacgc ctttggcgtt cgcggtgcgc acgtagtctt cgccgagaac ctccagcatg
     9721 ctgccacgca tgatccgggt gatgacgccg agcgtgtagg atgagaggac cagcgccggc
     9781 agaaccaggt ggtggagcca gtcgaggagc gcgtccgtat cgtgcgccag cagcgcgtcg
     9841 atgagcacaa acccgctgac cgggagtggc ggagcgaggc tggggctcag acgcccgggc
     9901 gccggcgccc agcccagacg cgcgtagaag atgacgatgg agacgatgcc gagccagaag
     9961 accggtatgg agacgccgac cagcgcactc atgcgcgccg cctggtcgat cggattctcg
    10021 cggttcaccg ccgccaggat ccccaggggg atgccgatga ggatgctgat cgccagcgcc
    10081 gatgtggcga gctcgatggt ggcgggcagg tgctgcttga gatcgagcgt gacgggctgc
    10141 cgggtcgaga tggacgtccc catgtccccg tgggcaagat gccagaggta gaccaggtac
    10201 tgcttgtaga ggggctgatc cagcccccat cggtgccgga acgcttccac cacgaccggg
    10261 tcggacgccg cctgctcgcc tatgttggca gccaccggat cggccggcac ggcgtgcgac
    10321 aggatgaagg tgagcagggt gatcccgatg agcatcgggc ccagcagcag cacccgccgc
    10381 aggacatagc gctggagatt catggccgcc ctatggggga aggccgagcc aaacggggcg
    10441 tggcgccgcg caggggctag gcggcgactt tgcccgcctt gaaatcggca atgaagtggt
    10501 cgatgtgctc gaggcccagc cgaattgtct cggccggcgt tcccggcccg aaccccaggc
    10561 ggacgtgccc ctcgcatcca tagcagctcc cggggatcag gaccacccgc tcccccaggc
    10621 gcacggccag cacctgctcc atgatccgct tggcggtggg agccatatcg cgttcaatat
    10681 tcgcagcggc gcctccaggg ggaaacgtct gaagcctgcg cattatacgg cgatgcgccg
    10741 tgcgcaacat cgattcctgc ctggcggtag aacggccggc gctaccgccg cgatgggcgg
    10801 gacagggcgg gaatcacaag acggtctggc ggcaggtcac cacagctttt cttgaagggt
    10861 gatgcccggc agggtcgcga ggtcgagcgg atcttcgggc ttgacgccca ggtcgtgctc
    10921 catcaaccag tagatctggc gcagatggtg ggccgagtgg tcggcaagcc aggtgaccgc
    10981 atcggccagg ctcattgcac catcgggcgt ctcgacgatg cgggtgtaat ccccccgacc
    11041 gggggagaac cgcgcccgca gcgtcgcccg gctggcttcg ccgtacctga gaaggctcgc
    11101 catgtcggga tagcgcgcgg catccttctt ctgggtggcc gcgtcacgcc gtgacacccc
    11161 gccgtcctcc gggacccagg tcagggcttt gtagaaaacg tcatggatga gctccccgag
    11221 ggtcctatcg cgattcggca tcttcaggat gaggccggcg ttcccgagtt gggcggcgac
    11281 cctcaggatg gccgcaagca ggcgatcgaa gccattcaac accgcctcca cgttgggccc
    11341 ggtcgaccgg tccccgacgc cgatggctgc gcccagcgcc ctggcgttga accccgggat
    11401 cggtggttga cccggcagca cgaccaccgg gaccgtcaga aaccccaact cggccagctc
    11461 ccgggcggcg gcgggatcgt tgtcgacgtc gcggggcagg taggcgatgt ggtggcgcga
    11521 aaggaactcc ttcgcctgga tgcaggcgaa tcagcccgga cggtagtaga ctctggctac
    11581 ttcagaagga atcgcgcgtt cctccttccg ccccgagccg gtgtgcgcag aacaccgcca
    11641 gtatatggtc accgcgttgc gcattgcaag cgggcgccgc gttcaacccc gcaacgccgc
    11701 aagcgtctgc ggaccgacga tcccatccac cacgaggttg gtcgccccct ggaaggcgat
    11761 gacggccgcc ctcgtttcgg ggccgaagac gccgtctgcg gggccggggt cgaatccttt
    11821 ggccctgagg cgcgactgga tctcctcgac ctcggggccg gcgtcacctt cggagatgaa
    11881 ggcgtcgccg gcgccgcccc cggacggggg ccggcccgtg gccacgaagg cggcgaggtc
    11941 ctcgggcgag ccgttgaaga tgttgagatc caccggccca ttgatcccgc gcacgctccc
    12001 gccggacgta tactgccaga gcgtgtacgt gctccacccg cgcgggatga tcggcgcgct
    12061 gcgcccgtag tcggcgatcc acagcggata ctcggagaac ccccgagcac tccccaaccc
    12121 gtccagaaaa ctcggggagg catagatcat gggcttccgc cccatcgccg cctccaccgc
    12181 atccagccac cgctgcatcc gctggaccaa ggtgggggcg tccacgccgt ctcgcacttc
    12241 cacgtcgagg gccgggggca gatcatcggg cccgggtgcg accgtcgcca aaaggtgctc
    12301 ggcctggatt tgggggtcga cgagcggccg aaagtagtgg tacgcaccgc ggacaagccc
    12361 ggccgcctgg atgccggccc agttgcgggc gaacgtctcg gccacgaagt ccgcaccctc
    12421 ggtggccttc gcaatggcga accggacgcc cgcggcggcg acggccggcc agtcgaccgc
    12481 accctggtaa tctgacacat ccacgccggc gacgctcatc gatctagacc ctcgtgccgg
    12541 cgaccatcac accgggatcg gcgttggctg ggccaagcgc ggtggtgcgt taaaatcaaa
    12601 gcagtcccac atatcatcgg a
//
LOCUS       DASQGH010000066         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22955, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000066 DASQGH010000000
VERSION     DASQGH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22955"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VEW91_01215"
     CDS             <1..306
                     /locus_tag="VEW91_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM90233.1"
                     /translation="YRFRGHSMADPEQYRTKEEVASWRERDPIPAFGDLLVGEGVIEE
                     ADRERIDVDAMKRVDAAVAFAEASPFPAPESLYDDVYVLDQEVRGWYSVETTDARFA"
     gene            306..1304
                     /locus_tag="VEW91_01220"
     CDS             306..1304
                     /locus_tag="VEW91_01220"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYM90234.1"
                     /translation="MAQMRYREALNAALREELARDERVVLMGEDIGVFGGAFKVTDGL
                     LEEFGEKRVRDTPISENTIVGMGVGAAMTGLRPVVELMTINFALLAFDQIVNHAAHIH
                     YMFGGQAQVPLVVRMPQGAGHQLGPTHSHCLEALFAHVPGLLVATPATPADAKGLLKT
                     AIRDENPVVFIEHESLYGVRGEVPEDEEELVHFGLARVAREGSDVTIVAISRMALTAE
                     RAAETLAAEHGVEAEVIDPRTLRPLDLDTILASVRKTNRCVIVEEGWPHGGVGANLAA
                     LIGEQAFDDLDAPVQRVAGADVPMPYSKPLEDIAYPHEPQIVHAALAALNRVAGAA"
     gene            1304..>1979
                     /locus_tag="VEW91_01225"
     CDS             1304..>1979
                     /locus_tag="VEW91_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HYM90235.1"
                     /translation="MEEIVMPRLSDTMEEGTILRWLKGDGEEVARGEELVEIETDKAT
                     MIYESDQVGVLQIVAAEGDTLAVGAVIARVGEAGDGAGAEPAAASPSAVNDEGPAPGG
                     PARVPAGEAPAEDRSHGATAPSERIKASPLARRIAGESGIDLQGVTGSGPGGRIVKAD
                     VVGSAGEPAQAPVPDVMTAKGETTTVELSRIEQTIARRMAESKATIPDFTLVIDVDME
                     ECVGLRT"
BASE COUNT          303 a          630 c          795 g          251 t
ORIGIN      
        1 taccgcttcc gcgggcactc gatggccgac cccgagcagt accgcacgaa ggaggaggtg
       61 gccagctggc gcgagcgcga cccgatcccc gcgttcgggg acctgctggt cggcgagggg
      121 gtcatcgagg aggccgaccg cgagcggatc gacgtcgatg cgatgaagcg agtcgacgcg
      181 gccgtggcct tcgccgaggc ctcgcccttc ccggcgccgg agtcgctgta cgacgacgtg
      241 tacgtgctcg accaagaggt gcggggctgg tactcggtgg agaccaccga cgcgaggttc
      301 gcctgatggc gcagatgcgc taccgcgagg ccctgaacgc ggccctgcgg gaggagctgg
      361 cgcgggacga gcgggtggtg ctgatgggcg aggacatcgg cgtgttcggg ggcgccttca
      421 aggtgacgga cggcctgctg gaggagttcg gcgagaagcg cgtacgcgac acgccgatct
      481 cggagaacac gatcgtgggc atgggggtgg gcgcggcgat gaccggcctg cggcccgtgg
      541 tggagctgat gactatcaac ttcgcgctgc tggccttcga ccagatcgtc aaccatgcgg
      601 cccacatcca ctacatgttc ggcggccagg cgcaggtgcc gctggtggtg cggatgcccc
      661 agggggccgg ccaccagctc gggccgaccc actcgcactg cctggaggcg ctgttcgccc
      721 acgtccccgg gctgctcgtg gccacgccgg ccacccccgc cgacgcgaag ggactgctga
      781 agacggcgat ccgggacgag aacccggtgg tgttcatcga gcacgagtcc ctgtacgggg
      841 tgcgcgggga ggtgcccgag gacgaggagg agctggtgca cttcggcctg gcccgcgtgg
      901 cccgggaggg ctccgacgtg acgatcgtgg ccatctccag aatggcgctg acggcggagc
      961 gggcggcgga gacgctcgcc gccgagcacg gggtggaggc ggaggtgatc gacccgcgga
     1021 ccctgcggcc gctggacctc gacacgatcc tggcctctgt gcggaagacg aaccggtgcg
     1081 tgatcgtgga ggagggctgg ccccacgggg gagtgggggc gaacctcgca gcgctgatcg
     1141 gcgaacaggc cttcgacgac ctcgacgcgc ccgtgcagcg ggtcgccgga gcggacgtgc
     1201 cgatgcccta ctccaagccg ctggaggaca tcgcctaccc gcacgagccc cagatcgtgc
     1261 acgcggccct ggccgccctc aaccgcgtgg ccggagccgc ctgatggaag agatcgtgat
     1321 gccgcgcctg tcggacacga tggaggaggg gacgatcctg cgctggctga agggggacgg
     1381 cgaggaggtg gcccgcggcg aggagctcgt ggagatcgag acggacaagg cgacgatgat
     1441 ctacgagtcc gaccaggtgg gcgtccttca gatcgtggcc gccgagggcg acacgctggc
     1501 ggtcggagcg gtcatcgcgc gcgtgggcga ggcgggcgac ggtgcgggag ccgagccggc
     1561 cgcggcctcc ccctcggcgg tcaatgatga ggggccggcc cctggagggc cggctcgcgt
     1621 gccggccggt gaggcccccg cggaggaccg ctcgcacggg gccacggctc cgtccgagcg
     1681 catcaaggcg tcgccgctcg cccggcgcat cgccggcgag agcgggatcg acctgcaggg
     1741 cgtgacgggc agcggcccgg gcgggcggat cgtcaaggcc gacgtggtcg ggtcggcggg
     1801 cgagccggcg caggctcccg tcccggacgt gatgacggcc aagggcgaga cgacgaccgt
     1861 ggagctgtcg cgcatcgagc agacgatcgc gcggaggatg gccgagtcga aggcgacgat
     1921 ccccgacttc acgctggtga tcgacgtgga catggaggag tgcgtggggc tgcgcacgg
//
LOCUS       DASQGH010000067         7031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5942, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000067 DASQGH010000000
VERSION     DASQGH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5942"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1618
                     /locus_tag="VEW91_01230"
     CDS             77..1618
                     /locus_tag="VEW91_01230"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HYM90236.1"
                     /translation="MPIVPARSADLPLVLALDLGSSWLRAIVYDAQGREVAGTEGRTA
                     WRWTRTPDGGVEGDPDALLAGAFEAVDRAIAGAGGAAQEIRAVGVSTFWHNILGVGPD
                     GRPTTPLYSWADERSAEAARLLRHRMDERTVHRRTGCVFHPSYPAARLLWLRGARPDA
                     WRATRTWMSIGEYLALRCFGRAVCSVSMASGSGLLDQDRCEWDAEVLGAIGLTPDRLA
                     PLADFKDPLAGLASASAARWPALTHVPWLPAAGDGALSNLGTGCVGPSRAALAVGTSG
                     AMRVLCRTAPAGLPPGLWRYRLDRDRRLVGGAVSNGGSIFRWMQEQFALGAPEAIERE
                     LRLRRPDGHGLVVLPFLAGERSPHWPLRARGAVVGMTLATDPYDLLQAGLEAVAHRFA
                     LIWDQVRETVPEVREIVASGGALLHSPAWMQMIADVLGHEVIASAEAEGSSRGAALLA
                     LELLGTVRAEDVPAPLGSVCSPDPERHARYREARTRHLRVEAVLGPLQEAVADNGGSP
                     PDRVE"
     gene            1693..3804
                     /gene="tkt"
                     /locus_tag="VEW91_01235"
     CDS             1693..3804
                     /gene="tkt"
                     /locus_tag="VEW91_01235"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYM90237.1"
                     /translation="MATKIQTVEDLCINTIRTLSIDAVQKANSGHPGLPMGAATLGYV
                     LWTRHLRHNPRNPQWPDRDRFVLSAGHGSMLLYSLLFLTGYDLTLDDIREFRQWNSRT
                     PGHVEHGPTPGVEATTGPLGQGFGDSVGMAIAERWLAARFNRPDHTVIDHYTYVLASD
                     GDLMEGVASEAASLAGHLRLGRLIVLYDANEVTLSATTNVTFTEDVGARFEAYGWHVQ
                     RIDGHDSKAVDAALAVARAVEDRPSLIVARTHIGYGSPNRQDTYKAHGEPLGEEEVRL
                     TKRALGWPEDRTFYVPEEALREFRRGIERGAEWEAAWRRRIDAHRAAHPDLAAQLAQA
                     LAGDLPGGWEAHLPTFAAKDGPIATREASGKILNAIAGAIPNLVGGSADLDPSTHTAM
                     KGEGDFESPTAGVGDAGGLRPQGTAGGVWAYAGRNIHFGIREHAMGTILTGMALHGGV
                     VPFGATFLTFSDYMRPSVRLAAMSEARVIYVWTHDSIGLGEDGPTHQPVEHVISLRAI
                     PNLIVIRPADATETVEAWRIALGHRAGPVALVLTRQKLPVLDRQTLAPAAGVVRGGYV
                     LAEPSGGSPPDVILVATGSEVPVALAAHEQLARDGVRSRVVSMPSWELFEAQPQAYRD
                     AVLPPSVRARVSVEAGSPIGWERYVGPEGVIIGVRRFGASAPGAVVMREFGFTAEHVA
                     DAAKAVLARVRPREEKGRRTR"
     gene            3801..4943
                     /gene="tal"
                     /locus_tag="VEW91_01240"
     CDS             3801..4943
                     /gene="tal"
                     /locus_tag="VEW91_01240"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HYM90238.1"
                     /translation="MKEMTVQNPLLALRKAGQSVWLDFIQRNLLRSGELRRLVDDDGV
                     AGVTSNPTIFEKAISESRDYDAAVARLRARGAGAAEAYETLIAEDITAACDILRPVFE
                     RTGGTDGFVSVEVSPLLAHDADGTIREVRHWCERIGRPNLMVKIPGTAEGVPAIEAML
                     AEGRNINITLLFSVQAYARVAEAYLRALERRVAAGQPVDRVASVASFFVSRIDTETDK
                     RLEVRLGGAPDASERERLRSLLGKAAIASARLAYRMFREVFSSPRFKALAARGARVQR
                     PLWGSTSTKNPAYPDLLYVEALVGPDTVDTMPPQTLAAFRDHGRVNPRAVMEGLDEAT
                     AVLARLAEVGIPIEDVTQRVLDAGVKSFADSYSQLLNVIEQRLGAA"
     gene            4974..5870
                     /gene="gnd"
                     /locus_tag="VEW91_01245"
     CDS             4974..5870
                     /gene="gnd"
                     /locus_tag="VEW91_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYM90239.1"
                     /translation="MKLGFIGFGRMGGNMVRRLLGRGHEITVYARRAEVRAEAAGLGA
                     TPAASLADLVDRLAAPRVVWMMIPAGDAVDTTLGELTPLLARGDIVVDGGNSNYRDSI
                     RRAEAAKARGVHYIDAGTSGGIWGLQIGYCMMVGGAAEAVRVVEPVFKALAPEGGYLH
                     AGPSGAGHFVKMIHNGIEYGMLQAYAEGFAILHDGPYKIDLRAVSALWNHGSVVRSWL
                     LELAERAFTADPELRSLRGYVEDSGEGRWTVQEAIDRSVPAPVITLSLLQRFRSRQEE
                     NFGDKVIAALRQQFGGHAVREA"
     gene            5877..>7031
                     /gene="zwf"
                     /locus_tag="VEW91_01250"
     CDS             5877..>7031
                     /gene="zwf"
                     /locus_tag="VEW91_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYM90240.1"
                     /translation="MAAVSIQSAIREPRRSRRTPDPCTVVIFGATGDLTQRKLMPALY
                     TLARIGMLPAESAVVGFARRPMNDGAFRSEMAKSVLGSASPGPDPAAWEGFARRLFYV
                     QADFHDPAGYEQLRATLERLERERGTGGNRLFYLATGPELYHEIITRLGEHRLVHRPA
                     PGTDGDRPWTRVIVEKPFGRDLASAQELNHTLLRVFRESQIFRIDHYLGKETVQNILV
                     FRFANGIFEPIWNQHYVDHVQITVAETVGVEGRAGYYETAGVVRDIIQSHMMQLLTLM
                     AMESPVAIEPDAVRSEKVKVLRAARRIVPEGVARHVVFGQYGPGAVDGQAMPGYLEEP
                     RVAPHSRTPTFVAMRLFIDNWRWASVPFYLRTGKRLPKRVTEVAVQFKRAP"
BASE COUNT         1009 a         2354 c         2654 g         1014 t
ORIGIN      
        1 ggtacggccg ctcgttaaga tctttgatga agggactccg atactcgatg gaccctcata
       61 gcccagggaa cggcccgtgc ccatagtgcc cgcccgaagc gcggacctcc cgctcgtgct
      121 ggcgctcgac ctcggcagct cctggctccg cgccatcgtg tacgacgccc aggggcggga
      181 ggttgcgggg accgaggggc gcaccgcatg gcggtggacc cggaccccgg acggcggcgt
      241 cgagggggat ccggatgccc tgctcgcggg cgcgttcgag gcggtggatc gggcgatcgc
      301 gggggcgggg ggggcggcgc aggagatcag ggcggtcggg gtctcgacgt tctggcacaa
      361 catcttgggg gtcggccccg acggccgccc caccaccccg ctgtattcgt gggcggacga
      421 gcgcagcgcg gaggccgcgc gtctgctgcg gcaccggatg gacgagcgca cggtccacag
      481 gcgaaccggg tgcgtgttcc acccgagcta tcccgccgcc cggttgctct ggctgcgcgg
      541 agcccggccg gacgcgtggc gggccacccg gacctggatg agcatcggcg agtacctggc
      601 ccttcgatgc ttcgggcgcg cagtgtgcag cgtttcgatg gcgtcggggt ctgggctcct
      661 ggaccaggat cggtgcgagt gggacgcgga ggtgcttggg gccatcggcc tcacgccgga
      721 tcgactcgcg ccgctggccg atttcaaaga cccgctcgcc gggctggcct cagcctccgc
      781 cgcgcgctgg ccggcgctca cccacgtgcc gtggcttccg gcggccggcg acggggccct
      841 gagcaacctc ggcacgggat gcgtcgggcc ctcgcgcgcg gcgctcgccg ttggcacctc
      901 cggagcaatg cgggtgctgt gccggaccgc ccctgccggg ttgccgcccg ggctgtggcg
      961 gtatcgcctc gatcgggatc ggcgcctcgt cggcggcgcg gtcagcaacg ggggaagcat
     1021 ctttcggtgg atgcaggaac agttcgccct cggcgccccc gaggcgatcg agcgcgagct
     1081 caggctgcgc cggcccgacg gccacggcct tgtggtgctg ccctttctgg cgggggagcg
     1141 cagtccccac tggccgctgc gggcgcgcgg ggccgtggtg ggtatgacac tggccacgga
     1201 cccgtacgac ctgctgcagg ccggtctgga ggcggtcgcg caccggtttg cgttgatctg
     1261 ggaccaggtc cgggagactg tccccgaggt ccgcgagatc gtcgccagcg gcggcgccct
     1321 cctgcattcg cccgcatgga tgcagatgat cgccgatgtg ctgggccacg aggtgatcgc
     1381 gtcggccgag gcggagggca gcagccgggg ggcggccctc ctcgcgctcg aactgttggg
     1441 cacggtccgc gccgaggacg tgccggcgcc tttgggatcg gtgtgctcgc ccgatcccga
     1501 gcggcacgct cggtaccggg aggcgcggac gagacacctg cgggtcgagg cggtgctggg
     1561 ccccctgcag gaggccgttg cggacaacgg cggttccccc ccagatcggg tagaataggt
     1621 gggaccgggc tcgcacccgg ggggcggcgg ccgccgccgc gggtgcgtga agccggggtt
     1681 ggaggacgta cgatggccac gaagattcag acggtggaag atctctgcat caacacgatc
     1741 cggaccctgt ccatagacgc cgtccaaaag gcgaactcgg ggcacccggg ccttcccatg
     1801 ggcgcggcca cgctggggta cgtgctgtgg acccgccacc tgcgccacaa cccgcgtaac
     1861 ccccagtggc ccgaccgcga ccggttcgtg ctctccgcgg gccacgggag catgctgctc
     1921 tacagcctct tgttcctgac ggggtacgat ctgacgctgg acgatatcag ggagttccgt
     1981 cagtggaaca gccggacccc cggccatgtc gagcacggac ccacgcccgg ggtcgaggcg
     2041 accacgggtc ccctggggca ggggtttggg gacagcgtcg ggatggcgat cgcggagcgg
     2101 tggctggcgg cccgcttcaa ccgcccggac cacaccgtca tcgatcacta cacgtacgtg
     2161 ctcgcgagcg acggcgatct gatggagggg gtcgcctcgg aggcggcctc cctggccggc
     2221 cacctccggc tcggccgcct catcgtgctc tacgatgcga acgaagtcac gctttcggcc
     2281 accacgaacg tgacgttcac cgaggacgtg ggcgcgcggt tcgaggcgta cgggtggcac
     2341 gtccagcgga tcgacgggca cgatagcaag gccgtggatg ccgcgcttgc ggtcgcgcgg
     2401 gcggtcgagg accggccctc gctgatcgtg gcgcggacgc acatcggcta cgggagcccg
     2461 aacaggcagg atacgtacaa ggcgcacggg gagccgctcg gcgaagagga ggtgcggctc
     2521 acgaagcggg cgctcggctg gccggaagac cgcacctttt acgttccgga agaggccctg
     2581 cgcgagttcc gccgggggat cgagcggggc gcagaatggg aagcggcctg gcggcggcgg
     2641 atcgatgcgc accgcgccgc gcacccggac ctggcggctc aattggcgca ggccctggcg
     2701 ggcgatctcc ccgggggatg ggaggcccac ctccccacct ttgccgccaa ggatgggccg
     2761 atcgcgaccc gcgaggcctc gggcaagatc ctcaatgcca ttgccggcgc catccccaac
     2821 ctggtcggcg gatccgcgga tctcgatccg tcgacccaca ccgcgatgaa aggggagggc
     2881 gacttcgaga gccccaccgc gggtgtcggg gacgccggcg gactccggcc ccaagggacc
     2941 gccggcggcg tctgggcgta tgccgggcgg aacatccact tcgggatccg cgagcacgcg
     3001 atgggcacga tcctgaccgg gatggcgctc cacggtggag tcgtcccgtt cggggcgacg
     3061 ttcctgacct tttccgacta tatgcggccg agcgtgcggc tggccgcgat gagcgaggcg
     3121 cgggtcatct atgtctggac ccacgacagc atcggcctgg gggaggacgg cccgacgcac
     3181 cagccggtgg agcacgtgat cagcctccgg gcgattccca acctgatcgt catccggccg
     3241 gctgacgcga ccgagaccgt cgaggcgtgg cggatcgcgc tcgggcaccg ggccggaccg
     3301 gtcgccctgg tgttgacgcg tcagaaactc ccggtgctcg accgtcagac cctcgcgccg
     3361 gcggccgggg tggtgcgcgg ggggtacgtg ctggccgagc cgtcgggcgg ctcaccgccc
     3421 gacgtcattc tggtcgccac cggatcggag gtgcccgtgg cgcttgcggc gcacgagcag
     3481 ctggcgcggg acggcgtccg cagccgggtg gtgtcgatgc cctcctggga gctcttcgag
     3541 gcccagcccc aggcctaccg cgatgcggtt ctgccgccgt cggtgcgcgc cagggtgagc
     3601 gtggaggcgg ggtcgccgat cggatgggaa cggtacgtcg gtcccgaggg cgtgatcatc
     3661 ggggtgcggc gcttcggcgc ctcggcgccc ggggcggtgg tgatgcggga gttcggcttc
     3721 acggccgagc acgtggcaga cgctgccaag gcggtcctcg cccgggtgcg cccacgcgag
     3781 gagaagggac ggagaacgcg atgaaagaga tgacggtgca gaatcctctg ctggccctgc
     3841 gcaaggcggg gcagagcgtg tggctcgact tcatccagcg gaacctgctt cgctcgggtg
     3901 agctccgtcg gctggtcgac gacgatggcg tcgccggagt gacgagcaac ccgaccatct
     3961 tcgagaaggc gatctccgag agccgggact acgatgccgc ggtcgcgcgg ctccgggccc
     4021 gcggcgcggg ggcggccgag gcgtacgaga cgctcatcgc cgaggacatc accgcggcgt
     4081 gcgacatcct ccgccccgtc ttcgagcgga ccggggggac ggacgggttc gtctcggtcg
     4141 aggtctcccc gctcctcgcg cacgacgccg acggcacgat ccgcgaggtc cgccactggt
     4201 gcgaacgcat cggccggccg aacctcatgg tgaagatccc gggcacggcg gaaggggtcc
     4261 cggcgatcga ggcgatgctc gcggagggca ggaacattaa catcacgctg ctgttcagcg
     4321 tccaggcgta cgcgcgggtg gcggaggcct acctccgggc gcttgaacgc cgggtggccg
     4381 ccggccagcc ggtggaccgg gtggcgtcgg tggcgagctt cttcgtcagc cggatcgaca
     4441 ccgagaccga caagcggctc gaggtgcggc tggggggcgc cccggatgcc tccgagcgcg
     4501 agcggctgcg gagcctcctc ggcaaggccg cgatcgccag cgcccggctc gcctaccgga
     4561 tgtttcgcga ggtgttttca agcccccggt tcaaggccct ggccgcccgc ggcgcgcggg
     4621 tccagcggcc gctctggggt agtaccagca ccaagaaccc cgcgtacccc gacctcctct
     4681 atgtcgaggc gctggtgggt cccgacacgg tcgacacgat gccgccccag acgctcgcgg
     4741 cgttccgcga ccacggccgg gtgaaccccc gggccgtcat ggaagggctc gacgaggcga
     4801 ccgcggtgct ggcccgcctt gccgaggtcg ggattccgat cgaggacgtc acgcagcgcg
     4861 tgctcgacgc cggggtcaag tcgtttgccg actcgtacag tcagctcctc aacgtcatcg
     4921 agcagcgcct cggggctgcg tagccctgac gcgggcgggg gagggatgcg cgcatgaagc
     4981 tgggattcat cgggtttggc cggatgggcg ggaacatggt gaggcgcctc ctcggtcgcg
     5041 ggcacgagat cacggtgtat gctcggcggg ccgaggtgcg cgccgaagcc gccggcctgg
     5101 gcgccacccc ggcggcgtcg ctcgccgacc tggtcgaccg gctggccgcc cccagggtcg
     5161 tgtggatgat gatcccggcg ggcgacgccg tcgatacgac cctcggagag ctgaccccgc
     5221 tcctggcgcg gggggacatc gtcgtcgacg gcggcaactc caactaccgg gattccatcc
     5281 ggcgggccga ggcggcgaag gcccgcggcg tgcactacat cgacgccggg acgagcggcg
     5341 gcatctgggg gctgcaaatc gggtactgca tgatggtcgg aggggcggcg gaggccgtgc
     5401 gggtggtgga gccggtgttc aaggccctgg cgcccgaggg cgggtatctc cacgccggcc
     5461 cgagcggggc cgggcatttc gtcaagatga tccataacgg catcgagtac ggcatgctgc
     5521 aggcctacgc cgaagggttc gcgatcctgc acgatgggcc gtacaagatc gacctccggg
     5581 cggtcagcgc cctctggaac cacggcagcg tcgtgcggtc ctggctgctc gagctggccg
     5641 agcgcgcgtt taccgcggat cccgagctcc ggtcgctccg cggatacgtg gaggattccg
     5701 gcgaggggcg gtggacggtg caggaggcga tcgaccgcag cgtgccggcg ccggtgatca
     5761 cgctctccct gctgcagcgc ttccggtccc ggcaggagga gaacttcgga gacaaggtaa
     5821 tcgccgccct gcgccagcag ttcggcggcc acgcggtgcg ggaggcctag ccaggcatgg
     5881 ccgctgtttc gatccagtcg gccatccgcg agccccgccg gtcccgccgc accccggatc
     5941 cgtgcacggt ggtgatcttc ggcgccaccg gcgatttgac ccaacgcaag ctgatgcccg
     6001 cgctgtacac gctcgcgcgg atcgggatgc tgccggccga gtccgccgtg gtcgggttcg
     6061 cccggcggcc gatgaatgac ggtgccttcc ggagcgagat ggcgaagtcg gtgctgggga
     6121 gcgcgtctcc cggccccgac ccggccgcgt gggaagggtt tgccaggcgg ctcttctatg
     6181 tccaggccga tttccacgat cccgcggggt acgagcagct gcgcgcgacg ctcgaacgcc
     6241 tggagcggga gcgcggcacc ggggggaacc ggctcttcta cctcgcgacg gggcccgaac
     6301 tataccacga gatcatcacc cggctcggag agcaccggct ggtccacagg cccgccccag
     6361 gcacagacgg ggaccggccc tggacgcgcg tgatcgtgga gaagccgttt ggccgggacc
     6421 tcgccagcgc ccaggagctg aaccacacgc tgctgcgggt ctttcgcgag agccagatct
     6481 tccggatcga ccactacctg gggaaggaga cggtccaaaa catcctggtc ttccggttcg
     6541 ccaacgggat cttcgagccc atctggaacc agcactacgt cgaccacgtc cagatcacgg
     6601 tggcggagac cgtcggggtc gagggacgcg cggggtacta cgagaccgca ggggtggtcc
     6661 gggacatcat ccagagccac atgatgcagc tgttgaccct gatggcgatg gagtcgccgg
     6721 tggcgatcga gccggacgcc gttcggtccg agaaggtcaa ggtcctccgg gccgcccggc
     6781 ggatcgtccc cgagggcgtc gcgcggcacg tggtgttcgg ccagtacgga ccgggcgcgg
     6841 tggacgggca ggcgatgccg gggtatctcg aggaaccgcg ggtcgccccc cactcccgga
     6901 cgccgacctt cgtcgccatg cggctgttta tcgacaactg gcggtgggcg agcgtgccct
     6961 tctacctgcg gaccggcaag cgcctgccga agcgggtgac ggaggtggcg gtccagttca
     7021 agcgcgcccc g
//
LOCUS       DASQGH010000068         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8796, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000068 DASQGH010000000
VERSION     DASQGH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8796"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VEW91_01255"
     CDS             complement(<1..1372)
                     /locus_tag="VEW91_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM90241.1"
                     /translation="MPRIVRQKIEFEGRIEERDVVIEGDDLPVWPADERFTIVGTPVP
                     RVDGRERVGGQARYTTDQFPAGMLWGAILRSPHAHARIARIETRKAERAPGVRAVLSS
                     VNAPKIRWHSGASWLFDPELRFVGDEVALVVAEDAAAARDTLDLIEVEYEVLPHVLDA
                     EEALRPGAVLVHPTGNILGGAWERYARGDADAGLAQAEVTVELVARTPDQLHHCLETH
                     GSVADWSGDRLVVWDSTQHIFGVRRQVATAFGIPLDRVRVLSPFMGGGFGSKNSAGKY
                     TVIAALAARATRRPVRIVFTRIEESQAAGKRPRSVQRIRLGARRDGRLTAIDYWALSN
                     VGAYRAFPSPLSGPPKELYACPNVRTETASVFTHTGPAAAFRAPGYVEATVGLECAME
                     ALADRLGMDPLEVRLRNHAEEDQVLGRAYSSKHLREAYQLGGRSIGWAKRTPTPAQPA
                     RGGKKRR"
     gene            complement(1388..1891)
                     /locus_tag="VEW91_01260"
     CDS             complement(1388..1891)
                     /locus_tag="VEW91_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYM90242.1"
                     /translation="MTPLAPAQPPTKIQLTVNGQPEEVAVAPRRTLLQVLREDLHLTG
                     TTDGCGIGTCGACTVLVDGRAMLSCLMLAVQASGRAVETIEAVGRDAADPLLAAFIRH
                     DAFQCGFCTPGQIMALRALFRLTPRPSIGEIRRAVEGNVCRCGAHLRIQAAALDVAAR
                     GGASNGA"
     gene            2053..2409
                     /locus_tag="VEW91_01265"
     CDS             2053..2409
                     /locus_tag="VEW91_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HYM90243.1"
                     /translation="MARVPVPVEITQDPPGHLGITWDDGHRSRYAYRTLRERCPCALC
                     VDEWTGEGRLDPSKVPDDIHPREVGRVGAYALKFTWSDGHLTGIYTFKFLREICECDP
                     CVRARAQAAPRPGTPP"
BASE COUNT          377 a          937 c          915 g          385 t
ORIGIN      
        1 cccggcgctt tttcccgccg cgggccggct gcgccggcgt gggggtccgc ttcgcccagc
       61 cgatcgagcg cccaccgagc tggtaggcct cccggaggtg cttgctcgaa taggcgcggc
      121 cgagcacctg atcctcctcc gcgtggttcc ggagccgcac ctccagcgga tccatcccca
      181 gccggtcggc gagcgcttcc atggcgcact ccagcccgac ggtggcttcg acgtacccgg
      241 gcgcgcggaa ggccgctgcc gggcccgtgt gcgtgaagac cgacgcggtc tcggtgcgga
      301 cgttcgggca ggcgtagagt tccttcggcg ggccgctcag gggcgagggg aacgcccggt
      361 acgcaccgac gttgctgagc gcccagtagt cgatcgcggt gagacggccg tcccgccgcg
      421 cgcccaggcg gatcctctgg acgctgcgcg gccgcttgcc cgccgcctgg ctctcctcga
      481 tccgcgtgaa gacgatgcgc accgggcggc gcgtcgcgcg cgcggcgagc gcggcgatca
      541 ccgtgtattt tcccgcgctg ttcttgctgc cgaacccgcc gcccatgaac gggctcagca
      601 cccgcacccg gtccaggggg atcccgaagg cggtggcgac ctggcggcgc accccgaaga
      661 tgtgctgggt ggaatcccac accacgagac ggtccccgga ccagtcggcg accgagccgt
      721 gggtctccag gcagtggtgc agctgatcgg gcgtccgcgc gacgagctcc acggtgacct
      781 cggcctgggc caaccccgcg tccgcatcgc cgcgcgcgta ccgctcccac gcgccgccga
      841 ggatgttgcc ggtgggatgg accaggacgg cccccggccg gagcgcctct tccgcatcga
      901 gcacatgcgg aagcacctcg tactcgacct cgatcaggtc cagcgtatca cgggccgccg
      961 cggcatcctc agcgacgacc agggcgacct cgtccccgac gaacctgagc tcagggtcga
     1021 acagccagct cgccccgctg tgccagcgga tcttcggcgc gttgacgctg gacaggaccg
     1081 cgcgcacccc gggagcccgt tcggccttgc gggtctcgat ccgggcgatc cgagcgtgcg
     1141 cgtgcgggct ccggaggatt gccccccaga gcatgcccgc agggaactgg tcggtcgtgt
     1201 agcgcgcctg tccccccacg cgctcccggc cgtccacgcg ggggacgggg gttcccacga
     1261 tcgtgaaccg ctcgtccgcc ggccagaccg ggaggtcgtc gccttcgatg acgacatccc
     1321 gctcttcgat cctgccctca aactcgatct tctgcctgac gatccgcggc atgcaggcct
     1381 cctaacctca ggcgccgttg gaggcgccgc cccgcgcggc gacgtccagg gcggcggcct
     1441 ggatcctgag atgcgccccg caccggcaga cgttaccctc gaccgcgcgc cggatctcgc
     1501 cgatggacgg ccgcggggtg agccggaaca gcgcccggag cgccatgatc tgccccggcg
     1561 tgcagaaccc gcactggaac gcgtcgtgcc ggatgaacgc cgccaggagc gggtcggcgg
     1621 catcccggcc gaccgcctcg atcgtctcga cggcccggcc ggatgcctgg acggcgagca
     1681 tcaggcagga cagcatcgcc cgaccgtcga ccagcaccgt gcaggcgccg cacgtgccga
     1741 tcccacaccc gtccgtggtc ccggtgaggt gcaggtcctc gcggagcacc tgcaaaagcg
     1801 tgcggcgcgg cgccacggcc acctcctcgg gctggccgtt cacggtcagc tggatcttgg
     1861 tgggcggttg agcgggagcg agcggggtca ctgtaacctc cgggatcgtg atcgggcaaa
     1921 cgacctgcag gattcggccc cttccgcgag ggctcctccg aggccgcgcg gaatggtgct
     1981 cgatgagggc acgtttacgc ctggagaacg acgggaaggt gccgccggat ttccacacaa
     2041 ccagggaggg gtatggcgcg cgtcccggtt ccggtcgaga tcacccagga tccccccggc
     2101 catctcggga tcacctggga cgacggccac cggagccggt acgcctaccg gacgctccgg
     2161 gagcggtgcc cgtgcgcgtt gtgcgtggat gaatggacgg gtgaagggcg cctcgatccg
     2221 tcgaaagtgc cggacgacat ccacccccgg gaggtcggcc gggtcggcgc gtacgccctt
     2281 aagtttacgt ggtcggacgg ccacctcacc gggatctata cgttcaagtt cctgcgcgag
     2341 atctgcgagt gcgacccctg cgtccgcgcg cgggcacagg ccgcaccgcg gcccgggacc
     2401 ccgccctagg actactacgt caggttctgg aaggaattga gccaggagct ggccgatcgt
     2461 agtgagaaga gtgcctccag tggactcctt cgacgtcatc aatgagggtc ccgacctccg
     2521 atgacagcgg gtccctgcac acggcgtagg ttcctgaagc gttccgctgc attacccggc
     2581 gcagtgcttg gggatgcgtt gtgggggggg gtcg
//
LOCUS       DASQGH010000069         8152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48388, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000069 DASQGH010000000
VERSION     DASQGH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48388"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..457)
                     /locus_tag="VEW91_01270"
     CDS             complement(41..457)
                     /locus_tag="VEW91_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYM90244.1"
                     /translation="MIAYIDASVLLRVALGQPDALPEWRQIDRGVASALVMTECLRTL
                     DRLRLRTKLSDAEVATHRATILALIASLELVEIDSVVLDRAAQPMPTELGTLDAIHLA
                     TALLWKDLTRADLTMATHDTALALGAQAHGLHVVGI"
     gene            complement(454..714)
                     /locus_tag="VEW91_01275"
     CDS             complement(454..714)
                     /locus_tag="VEW91_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYM90245.1"
                     /translation="MKQVRISELKAKLSEYLRAVRRGETIAVLDRETPVAQIVPVRAA
                     SALRVRKPAPGSPPPTRIPLPKPAKLSIDVVSLLLEERQGHR"
     gene            complement(814..2208)
                     /gene="glmM"
                     /locus_tag="VEW91_01280"
     CDS             complement(814..2208)
                     /gene="glmM"
                     /locus_tag="VEW91_01280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYM90246.1"
                     /translation="MGRLFGTDGIRGIANADLSTELVDRLARAAVEVLAGTVRGRFAL
                     GRDPRISGDLLEAAMAAGICSAGSDVLRLGVLPTPGVAHLARALAANAGIVISASHNP
                     VEDNGVKFFSETGFKLPDAVEAAIEAAMGGVLERSRPTGRDVGRIHEMPEAGERYLSY
                     VQGLARGRLGGWRVVLDCANGATSRIAPALWERLGATVIPLYAAPDGTNINVGCGSTH
                     PEVIQAAVVTHHADLGFAHDGDGDRVIAADRHGQLVDGDTIMGITALDRHARGQLPGN
                     LVVATVMSNLGLEQALRGAGIRLERVRVGDRYVLERMLETGAQVGGEQSGHVLFLDRA
                     TTGDGLVTAVELVNVILDSGRPLDDLRAPFIRYPQILMNVRVADPGRWASDPEIAKAA
                     RRAERRLGGRGRVLIRASGTEPLVRVMAEHEEAELAESIARELSDLIGRRLGGGASAP
                     LGKKQRPGSRLRPQ"
     gene            complement(2215..3426)
                     /locus_tag="VEW91_01285"
     CDS             complement(2215..3426)
                     /locus_tag="VEW91_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HYM90247.1"
                     /translation="MRRLWGERQLYFLLSVAIAVVMWLYVATAQNPQVSRPLNVDLQV
                     RNLGPNAVVIRPAPTAPVKVRVVLQGSRSQVAALTPKLVDAYVDLLGLGPGEHPQVPV
                     VVTAPPDLDVRVVDQNPTSLFFVLDAYASKRLPVEVSLLGRPPEGITIGNPRTSPTHV
                     IVSGPAHQVDEVRHALVSVDIATLRQEVGGSLPVIPADANGQAVAGVQVTPKLVGMTL
                     VVREGVLSKVVPIVPAIVGPPPQAFAVTSATAEPGTVALTGPGSVLRSIESAQTTPVD
                     VSRARGDFSRQVALQLPPGVTASVQQVTVTVHVGRGLLSTILRSVPVRVVGVPAGAAS
                     RVMPDRVDLQVEGPQDVVRHLTAQAVTVEVSAAGRPPGQYTMVPRAILPQGVHVLMIQ
                     PAQVVVIVSTS"
     gene            complement(3423..4283)
                     /gene="cdaA"
                     /locus_tag="VEW91_01290"
     CDS             complement(3423..4283)
                     /gene="cdaA"
                     /locus_tag="VEW91_01290"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HYM90248.1"
                     /translation="MPFALPSFRLSDIIDILVVSLIVFQVLMLVRRTRALQLAIGLGV
                     LFAAYVVSQRLQLYTLQWLLSRVGLVVPIALLVLFQPELRRMLEQLGRGGVSLVGFHF
                     AGLDREARIRLVNDTARAARILGSRKIGALIVLERRVGLADFIETGIQIDGVATVQLL
                     INIFFPNTPLHDGAVIIRGNRVVAAGCLLPLTGRPGLSRALGTRHRAAIGITEETDAV
                     AVVVSEETGTLSLAREGRLSRGLTEEELKVTLLALIGPQTRAAPFRVPALHVPVRVPE
                     FWQWGRRRSE"
     gene            complement(4285..5205)
                     /gene="argF"
                     /locus_tag="VEW91_01295"
     CDS             complement(4285..5205)
                     /gene="argF"
                     /locus_tag="VEW91_01295"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYM90249.1"
                     /translation="MRGRDFLSIRDLNPEELQHLLQRAAELKTRGKAGDRPTPLRGKV
                     LALVFEKPSLRTRTTFEAGMFLLGGHAVYLGPSDIQMGSRETPADVARNLERWVDGIA
                     ARTFAQSTVEALAEHAHIPVINALSDREHPCQALAALLTIQERFGRLEGIRLAWVGDG
                     NNVLRSLLYGGAQLGMAIAVATPNDYALDADTVRQGRALADAAHSVLEFTDDPVAAVR
                     DADVIYTDVWTSMGQEATAAARRRAFARYQVSAALLAHAPAHAVVTHCLPAHRGEEIT
                     DEVLDGPRSVAFDEAENRLHTQKALLAEVL"
     gene            complement(5395..5790)
                     /gene="rpsI"
                     /locus_tag="VEW91_01300"
     CDS             complement(5395..5790)
                     /gene="rpsI"
                     /locus_tag="VEW91_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYM90250.1"
                     /translation="MEELTQVSTSGGRKEAIARVRMVPGSGTIVVNGRSADKYFPKGP
                     LQTAIRQPLVVANAEGRFDVVAKVTGGGVSGQAGAIRHGIARALLALDAELRSPLRKA
                     GLLTRDPRTKERKKYGHKRARKGFQYSKR"
     gene            complement(5790..6245)
                     /gene="rplM"
                     /locus_tag="VEW91_01305"
     CDS             complement(5790..6245)
                     /gene="rplM"
                     /locus_tag="VEW91_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYM90251.1"
                     /translation="MRTMMATTAEAARVARWYLVDAKGLVLGRLASRIASVLRGKTRP
                     TFTPSVGGDFVIVVNADKIRLTGKKAQQKMYYRHSGYPSGIRATPAGKLLQRHPETVL
                     REAVRGMLPKTPLGRACLRKLRIYRGGSHRHAAQQPQALQLGPGQVGDR"
     gene            6398..7003
                     /locus_tag="VEW91_01310"
     CDS             6398..7003
                     /locus_tag="VEW91_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYM90252.1"
                     /translation="MALLRFGCALGTFGLLTWIASSPMIHRWDEWVTIWLQRTAPIPD
                     LPAAMLVLLGNAEAVILGLVLAAIVFWRQDRRRSTAALWLGGGVAIVGLVAVGLKHLI
                     VHPGPPPALQRIVLNAGLQLPTPYSYPSGHTLRTTLLAGTIFRRVPLFAAALVLGMMA
                     ALVYLGDHWLSDVLGGLCLGWASVEVAGAVRTARFRNCRRG"
     gene            complement(6966..7961)
                     /locus_tag="VEW91_01315"
     CDS             complement(6966..7961)
                     /locus_tag="VEW91_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYM90253.1"
                     /translation="MRASVSVNAIIPVAGRGKRIRPHTHTQPKVLMNVAGKPILAYIL
                     DELRDLGVEEIVLVVGHLAEKIEEYVREHYAFRAHFVRQDEPMGNGHAVYVAREYLTG
                     PTLIVFGDTVVQTDLRAAAGLPHSAIGVHRVEDPRAFGVVEVNKDGIVRHLWEKPEVP
                     PSDLAAVGVYMIQRPAPFRRALEELVEGRRMVDGEYWLADALQIMIDHGERLQTFQVG
                     EWYDCGTPEALLRANRALLDRLQPPTAQIAGSVIVPPSAIAATAVVEGSVLGPYVTVA
                     DGARVVNAVIQESIINANARVEDVLLEYSIIGDNAAVTGRRGRINLGDSSEIELS"
     gene            complement(7951..>8152)
                     /locus_tag="VEW91_01320"
     CDS             complement(7951..>8152)
                     /locus_tag="VEW91_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90254.1"
                     /translation="VLSPAIREHLPERGDHETTTFPALVRSGKLGAYKSQAYWRGVDT
                     IKDVNEVASEMQQRLMSLFREG"
BASE COUNT         1299 a         2884 c         2711 g         1258 t
ORIGIN      
        1 ggcctacagt ggcgcttgcg atagtatcat aatagccaaa ttaaatgccg acgacatgaa
       61 ggccgtgggc ctgcgcaccc agcgcgaggg cggtgtcatg agtcgccatg gtgagatcgg
      121 cgcgcgtcag atccttccag agtagggcag tggcgagatg gatcgcatcg agcgtgccga
      181 gttccgtcgg catcggttga gccgctcgat cgaggacaac cgaatcgatt tccacgagct
      241 cgagtgaagc gatcaacgca aggatcgtgg cgcggtgtgt cgccacttcg gcatcggaga
      301 gtttagtgcg gaggcgaagt cgatccagcg tacggagaca ctcggtcatg acgagggcgc
      361 tcgccacgcc ccggtcgatt tggcgccact caggcaacgc gtctggctgg ccaagcgcaa
      421 ctctcaacag gaccgaggcg tcgatgtacg cgatcaccga tgtccctgac gctcttcaag
      481 cagcagggaa acgacgtcga tggagagctt ggcaggcttc ggcagaggga tgcgagtggg
      541 gggtggcgag ccgggggccg gcttgcggac ccgcaacgcg gatgccgcgc ggaccggcac
      601 gatctgcgcg acgggtgtct cacggtcaag gaccgctatg gtttcaccgc ggcgcacggc
      661 tcgaagatat tcgctgagct tggccttcag ttccgagata cgcacctgct tcatgactat
      721 atgataacca gaattagtca ttctggcaag cctgaactca cccggctccg acgcagcacc
      781 tgatctcccg cggtcttgga cttcatggga ggtctattgg gggcgcaacc tcgagccggg
      841 gcgttgcttc ttgcccaatg gtgcactagc gccgcccccc agccggcggc cgatcaggtc
      901 ggacagctcc cgggcgatcg actccgccag ctcggcctcc tcgtgctcgg ccatcacccg
      961 gacgagcggt tccgtgccgg aggcccggat caggacccgc ccgcggccgc cgagacgccg
     1021 ctccgcccgc ctggccgctt tggcgatctc cggatcgctg gcccagcgcc ccgggtcggc
     1081 cacgcggacg ttcattagaa tctggggata ccggatgaac ggcgcgcgca ggtcgtcgag
     1141 cggccgcccg ctgtcgagga tcacgttgac gagttcgacc gcagtcacca acccatcgcc
     1201 ggtcgtggcc cggtccagga agagcacatg gccgctctgc tccccgccca cttgtgcgcc
     1261 ggtctccagc atccgttcca ggacgtaccg gtcgcccacc cggacccgct cgagcctgat
     1321 ccccgcgccc cggagggcct gctccagccc caggttgctc atcaccgtcg ccacaaccag
     1381 gttgccgggg agctggccgc gggcgtgccg gtcgagcgcg gtgatcccca tgatcgtgtc
     1441 gccgtcgacc aattgcccgt gccggtccgc ggcgatcacg cgatccccgt ctccgtcgtg
     1501 cgcgaacccc aggtcggcgt ggtgggtcac caccgccgcc tggatcacct ccgggtgcgt
     1561 cgagccgcat cccacgttga tgttcgtccc atcgggcgcg gcatacaggg ggatcaccgt
     1621 cgcccccagg cgctcccaca gggctggggc gatcctgctc gtcgcgccgt tcgcacaatc
     1681 caacaccacg cgccagccac cgaggcggcc gcgggcgagg ccctggacgt acgagaggta
     1741 gcgttcgccg gcctcgggca tctcgtggat ccgcccgacg tcgcgcccgg tggggcggga
     1801 gcgttccagc accccaccca tcgccgcctc gatcgcggcc tccacggcat ccgggagctt
     1861 gaagcccgtc tcagagaaaa acttcacccc gttgtcctcc acggggttgt gggaggccga
     1921 gatgacgatg cccgcgttcg cggccagcgc ccgtgccagg tgggcgaccc cgggtgtggg
     1981 gagcacgccc agccggagca catcggagcc ggcggagcag atcccggcgg ccatcgccgc
     2041 ttcgagcaga tcgcccgaga tccgggggtc gcgccccagc gcgaaccggc cgcgcaccgt
     2101 gcccgccagc acttccacag ccgctcgggc cagccgatcg acgagctcgg tcgagagatc
     2161 ggcgttcgcg atcccccgga tcccgtcggt cccgaacagc cggcccactg ccgcctacga
     2221 agtcgagacg atgaccacga cttgcgccgg ttggatcatc aggacgtgga ccccctgggg
     2281 aaggatcgcc cgcgggacca tggtgtactg ccccggcgga cggccggcgg cgctgacctc
     2341 aacggtcacg gcctgcgcgg tcaggtggcg cacgacgtcc tgcgggccct ccacctggag
     2401 atcgacgcgg tcgggcatca cccgggacgc ggcgcccgcc gggacgccca ccacccggac
     2461 cggcaccgac cggaggatcg tcgacaacag cccgcggcca acgtgcaccg tcaccgtcac
     2521 ctgctggacc gaggccgtca caccgggggg caactgcagc gccacctggc ggctgaagtc
     2581 accacgggct ctactcacat ccaccggggt ggtttgggcg ctctcgatgc tgcgcagcac
     2641 cgagccggga ccggtcaggg cgaccgtccc cggttcggcg gtggcgctgg tcaccgcgaa
     2701 ggcctgcgga ggagggccta cgatggcggg gacgatgggc acgactttgc tgaggacgcc
     2761 ctcgcggacc acgagcgtca tgcccacgag cttcggggtc acctggacgc ccgcgaccgc
     2821 ctggccgttc gcatcggcgg ggatcaccgg cagcgacccg cccacctctt gccgcagcgt
     2881 cgcgatgtcc accgacacga gcgcgtgacg gacctcgtcc acctggtggg ccggtccgct
     2941 gacgatcacg tgggtgggcg aggtgcgagg attcccaatg gtgatcccct ccggggggcg
     3001 cccgaggagg ctgacctcga ccggcaggcg cttgctggcg taggcatcca ggacgaaaaa
     3061 gagcgaggtc ggattctggt cgaccacccg cacatcgagg tccgggggcg cggtcaccac
     3121 caccggcacc tgggggtgct cgcccgggcc gagccccaac agatccacgt acgcgtccac
     3181 gagtttggga gtgagcgcgg cgacctggga gcgggacccc tggagcacga ccctcacctt
     3241 caccggcgcg gtgggggcgg gcctgatgac gacggcattg ggacccaggt tgcgcacctg
     3301 caagtccacg ttcaggggcc gggagacctg cgggttctgc gcggtggcga catacagcca
     3361 catcacgacc gcaatcgcga cggagagcag gaagtagagc tgacgctcac cccagagccg
     3421 cctcattcgc tgcgcctccg gccccattgc cagaactccg gcacccgcac cgggacgtgg
     3481 agcgcgggca cccggaacgg ggcggcgcgg gtctgggggc cgatgagggc caacagggtg
     3541 accttgagct cttcctcggt gagcccgcgg ctgagccggc cttcccgcgc gagcgacagc
     3601 gttcccgtct cctcggagac caccaccgcg accgcgtcgg tctcctcggt gatcccaatc
     3661 gccgcgcggt ggcgggttcc cagggcccgg ctcaggcccg ggcgccccgt tagcggcagg
     3721 agacaccccg ccgccaccac gcggttgccg cggatgatca ccgcgccatc gtgcaagggg
     3781 gtgttaggaa agaagatgtt gatcaggagc tggacggtgg cgacgccgtc gatctggatt
     3841 ccggtctcaa tgaaatcggc cagccccacg cggcgctcga ggacgatgag cgcgccgatc
     3901 ttccgggacc cgaggatgcg cgcggcccgg gcggtgtcgt tcaccaaccg gatccgcgcc
     3961 tcccgatcga gcccggcgaa gtggaagccg acgagggaga ccccgccgcg ccccagctgc
     4021 tcgagcatcc ggcgcagctc gggctggaac aggaccagca gggcgatcgg gaccacgagc
     4081 cccacgcggc tcagcagcca ctgcagggta tacagttgca gccgctggct caccacgtac
     4141 gcggcgaaca ggacccccag gccgatcgcg agctgcagcg cccgcgtgcg gcggaccagc
     4201 atgagcacct ggaacacgat caggctgacc accaggatgt cgatgatgtc ggagaggcga
     4261 aacgacggca acgcaaacgg catctcagag cacctcggcc aggagcgcct tctgcgtatg
     4321 gagccggttc tccgcctcgt cgaacgccac cgaccgggga ccgtccagga cctcgtcggt
     4381 gatctcctcg ccccggtgcg cgggcaggca gtgcgtcacg accgcatgcg cgggcgcgtg
     4441 ggccaggagc gcggcgctca cctggtatcg agcgaacgcc cgccgccggg cggcggccgt
     4501 ggcttcctgc cccatgctgg tccagacgtc cgtgtagatg acgtccgcgt cccggacggc
     4561 ggccaccggg tcatcggtga actcgagaac gctgtgcgcg gcatccgcca gcgcccggcc
     4621 ctggcggacg gtgtccgcat cgagggcgta gtcgttgggc gtggccacgg cgatcgccat
     4681 cccgagctgc gccccgccgt agaggagcga ccggagcacg ttgttgccat cccccaccca
     4741 ggccagccgg atcccctcga gccggccaaa ccgttcctgg atcgtcagga gcgccgcgag
     4801 cgcctgacac gggtgctccc gatcggagag ggcgttgatg acggggatgt gggcgtgctc
     4861 ggccagcgcc tcgaccgtgc tctgcgcgaa cgtccgggcc gcgatcccgt caacccaccg
     4921 ctctagattg cgggccacgt cggcgggggt ctcccgggac cccatctgga tatccgaagg
     4981 gccgaggtac accgcgtggc cccccagcag gaacatcccg gcctcaaacg tcgtgcgggt
     5041 ccggagggat ggtttctcaa acacgagcgc gagcaccttg ccgcgaagcg gcgttggacg
     5101 atccccagcc ttgcctcggg ttttcagttc ggcggcgcgt tggaggaggt gctgcaactc
     5161 ctcggggttg agatcacgga tactgaggaa gtcacgaccc cgcacgatct gtgcccaccc
     5221 ttcgcgcgtc cggtgccccc cgcccggcgc gagcaaaaaa gggcgcggcc agcgcccctc
     5281 cgtgtgcttc gccggacttg gccgattcct gtgggggcgc cggttcccgg ccgagggatc
     5341 tcgtaaaagg tgatgcccgt cagggtgacc ggctagaccg ggcgcctccc gcggctaccg
     5401 cttcgagtac tggaacccct tccgcgcgcg cttgtgaccg tacttcttgc gctccttggt
     5461 gcgcggatcc cgcgtcagga gaccggcctt ccggagcgga gaccgcaact cggcatcgag
     5521 cgcgagcagc gcccgcgcga tcccgtggcg gatcgccccg gcctgtcccg agaccccgcc
     5581 cccggtgacc ttcgcgacca cgtcgaaccg cccctcggcg ttcgcgacca cgagcggctg
     5641 gcggatcgcc gtctgcaggg ggcccttggg aaagtacttg tccgccgaac gcccgttgac
     5701 gacgatggtc ccgctgcccg gaaccatccg cacccgggcg atcgcttcct tgcgcccccc
     5761 ggatgtgctc acctgtgtca actcctccat taccgatcac ccacctggcc cggccctagt
     5821 tgaagcgcct gcggctgctg cgccgcgtgc cggtgcgatc cgccccggta gatcctgagc
     5881 ttgcgaagac aggcccgccc gaggggcgtc ttcggcagca tcccccgcac cgcctcgcgg
     5941 agcaccgttt ccgggtgccg ctggaggagc ttgcccgccg gggtcgcccg gatgccgctc
     6001 ggatagccgg aatggcggta gtacatcttc tgttgcgcct tcttcccggt gaggcggatt
     6061 ttgtccgcgt tcaccacgat cacgaagtcg ccgcccaccg acggcgtaaa cgtggggcgc
     6121 gtcttgcccc ggagcaccga ggcgatccgg ctggccaggc gccccagcac cagccccttg
     6181 gcgtccacca ggtaccacct ggcgacgcgc gctgcctcgg ctgtagtcgc catcatcgtc
     6241 cgcatttctg tcctccgact atctcccccg ggacctcaaa aaatggcccg ggccaccgat
     6301 tccgcggccc ggcgccttat tttacggtgc gcggggtcag gctgtcaatc acccctccgc
     6361 caggacgcgc cgcggatctc cccgaagagc taggctcatg gccctgctgc gcttcggatg
     6421 cgcgctcggg acatttggcc tgctcacctg gatcgcctcc tcccccatga tccaccgctg
     6481 ggacgaatgg gtgacgatct ggctccagcg gaccgcgccg atccctgacc ttccggccgc
     6541 gatgctcgtc ctgctcggga acgccgaggc cgtgattctg ggcctcgtcc tggcggcgat
     6601 cgttttctgg cggcaggatc gtcggcggag cacagctgcg ctctggctcg gaggaggggt
     6661 ggcgatcgtg gggctcgtgg cggtcgggct caagcacctg atcgtccatc ccgggccccc
     6721 gcccgcgctt cagcgcatcg tcctgaatgc cggcctgcag ctgccgacgc cgtacagcta
     6781 cccatccggc cacacgctca ggaccacgct cctggccggg acgatcttcc ggcgggtccc
     6841 gctgttcgcg gccgcgctcg tcctgggcat gatggccgct ctggtctacc tgggagacca
     6901 ctggctgtcc gatgtcctgg gaggcctgtg tctcgggtgg gcgtccgtgg aggtcgcagg
     6961 agcagtcagg acagctcgat ttcggaactg tcgccgaggt taatccgtcc gcggcgcccc
     7021 gtcaccgccg cgttgtcgcc gatgatcgag tactcaagaa gcacgtcttc tacccgggca
     7081 tttgcgttga tgatgctctc ttgaatcacc gcgttgacga cccgggcacc gtcggccacc
     7141 gtcacgtagg ggcccaggac cgagccctcc acgacggcgg tcgcggcaat cgctgaaggg
     7201 ggcacgatga ccgagccggc aatctgagca gtgggcggct gcagccgatc caggagggcc
     7261 cggttggccc gcaggagcgc ctccggggtc ccgcaatcat accattcgcc aacctggaag
     7321 gtctgaaggc gctccccgtg gtcaatcatg atctgcagcg cgtcggccag ccaatactca
     7381 ccgtcgacca tccgccggcc ctccacgagc tcctcgaggg cacggcgaaa cggcgcgggg
     7441 cgctgaatca tgtagacgcc taccgccgcc aggtcactcg gcgggacctc cggcttctcc
     7501 cagagatgac ggacgatgcc gtccttgttc acctcgacga cgccaaacgc ccgcgggtcc
     7561 tccacgcgat gcacgccaat ggccgagtgg gggagtccgg ccgcggcccg cagatccgtc
     7621 tgcacgaccg tatcgccaaa cacgataagg gtcgggccgg tgaggtactc tcgggcgaca
     7681 taaacagcgt gcccgttacc catgggctcg tcctgacgga caaaatgcgc gcgaaaagca
     7741 taatgctccc gcacgtattc ctcgatcttc tcggccaagt gccctaccac caagacgatc
     7801 tcctcaacgc caagatcccg caactcatcg aggatgtagg cgaggatcgg cttgccggcc
     7861 acattcatga ggaccttcgg ttgggtgtgg gtgtgaggcc gaatccgctt gccacggcca
     7921 gcaacgggaa tgatcgcgtt caccgagaca ctagccctca cgaaagaggc tcatcagccg
     7981 ctgctgcatc tctgacgcga cttcattgac gtccttgatc gtgtccaccc cgcgccagta
     8041 ggcctgggac ttgtacgcac ccaacttccc tgagcggacg agggccggga acgtggtggt
     8101 ctcgtggtcc ccccgctcgg gcaggtgctc ccggatggct ggcgacaaca cg
//
LOCUS       DASQGH010000070         8599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31472, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000070 DASQGH010000000
VERSION     DASQGH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31472"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..518)
                     /locus_tag="VEW91_01325"
     CDS             complement(180..518)
                     /locus_tag="VEW91_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90255.1"
                     /translation="MEVIVAAFLLTVGLVATAQLVLMATGQVALSRQQSDAAAIGAQT
                     IEQYRDINFGSLVAGTYTATPTVGATTYTVQTTVTVDDPQTGMKRVAVTVTWGGGKSY
                     ATSTILSPLQ"
     gene            complement(654..1115)
                     /locus_tag="VEW91_01330"
     CDS             complement(654..1115)
                     /locus_tag="VEW91_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90256.1"
                     /translation="MRARKLAGTSGKTLVEMAIILALMGILIRIALPALNAKPQNLAA
                     DMQDLSNNLQVARELGISRTQHYRVRVFSSGPPYVYVMERNSGPSWVTERTITLRPNV
                     RFDAGTLGLIAEFDTRGMLVTSPLPVFTLKDAARNWTKTVTVNGVGMVDHS"
     gene            complement(1270..1938)
                     /locus_tag="VEW91_01335"
     CDS             complement(1270..1938)
                     /locus_tag="VEW91_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM90257.1"
                     /translation="MILRGFPVGPIGANCYIFGDPAAREVFVIDPGDEPDRILETLRQ
                     LGARPVALVNTHGHFDHIQGVEAVRRATGAPFWIHEAEREVLTQGPARAKVLFGLDLP
                     PAPVPDRWLVEGDTLKVGRLTLTVRHTPGHSPGGVCLLGDGLAFVGDTLFAGSIGRTD
                     LPGGDLETLLASISRVLLPLPDETVCYPGHGPETTLGEEKRTNPFLAPLVHGPGGPAA
                     SGSL"
     gene            complement(1954..2496)
                     /locus_tag="VEW91_01340"
     CDS             complement(1954..2496)
                     /locus_tag="VEW91_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HYM90258.1"
                     /translation="MNLAGKQVAVLAEDYYENLELWYPVLRLREAGAQVTIVGPKAGE
                     SYKSKEGYPAKADLSMDDARAADFDAVIVPGGYAPDRMRRHPAMLRLVREAFQAGKVV
                     AAICHAAWVPISAGIVKGRTMTCVSAVKDDVINAGAHYVDKEVVVDGNLISSRTPPDL
                     PAFCREIVKALSEARVTARA"
     gene            complement(2577..3203)
                     /locus_tag="VEW91_01345"
     CDS             complement(2577..3203)
                     /locus_tag="VEW91_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HYM90259.1"
                     /translation="MAHPHRKLTPFGRYLLVQRIMELGWTVSAAAASAGVSRETAYKW
                     LRRWQHAGTEGLQDRSARPHTSPRCTPPATVQRLRRRRKLGPHRLGPLLGVARSTVYA
                     ILRREGLSRLRDADRTTGIPIRYVREHPGELIHLDMRLLARIPAGGGHRMLGRSPHTR
                     ALPKWLHHYNHDRPHTALGGQTPAARSVNNVYGNHRWRGRPSPIPPFA"
     gene            complement(3293..4162)
                     /locus_tag="VEW91_01350"
     CDS             complement(3293..4162)
                     /locus_tag="VEW91_01350"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYM90260.1"
                     /translation="MQRADRWEPGRPGGCSVLRSAWSKHLCAGLALAVALSVCGPVHA
                     GPGDAERLLRTGINAYRTGAPAHALQSFAEAAARAPHDALPALWAGVAAAAVGRWQDS
                     RAYLREALRRAHTPDEARLAEAWLLRLEAFDAPVPGAVDSAAGIAALAYASNPRLTWS
                     QARWLGQAVQAAAGREGLDPWLLASVIYIESRFNHQSISWAGAMGLGQLMPGTAQAAG
                     VDPKDPWQNVLGAAQILRWSYLEFKDWTLALAAYNAGGQAVRRYGGIPPYAETQWYVR
                     AVLWVYGQLRHPG"
     gene            complement(4274..5485)
                     /locus_tag="VEW91_01355"
     CDS             complement(4274..5485)
                     /locus_tag="VEW91_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM90261.1"
                     /translation="MGGGARGISRGLVRVAAIVGMAELGFATVIPLLPLYLKGRLGAS
                     ATLVGVVVATFAAVETVFKTTWGSLADRIGRRPVIVWGLFLSSTAPVIMTLLRSAWLF
                     VPLRLIDGMGSAALWPAAAAMMVDRTEDEQRATAMGTLNMFFLSGLALGPSLGLFVSG
                     FLGGYTWGFYLASALLLGAAALATLTFRDGERHAPAHHQPPGVWARGIFQGIRRSPSL
                     LAMLLVAFVQMFGVGLLAPILVIYAHEVVQLSDQLIGTMFLVMVLAVAIASVPGGRLA
                     DRVGRPRTVVWGMALASVGMWLLPLAGRGNLLVLGVAALLLGGSYAVSSPAWLALMSE
                     AAPRGSTGMVLGASETAQGAGLVIGPLLGGVLYDGVGPQAPFVASAALLTVGTCLALL
                     TLGRRRPTPAR"
     gene            complement(5490..6248)
                     /locus_tag="VEW91_01360"
     CDS             complement(5490..6248)
                     /locus_tag="VEW91_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HYM90262.1"
                     /translation="MSRIPELRICHLYPDLLNLYGDRGNIMVLMQRARWRGIPVTVIE
                     VGLGEAVDPDAADLFFIGGGEDRQQRIAADDLCRVKRAALEDAVLDGGVMLAVCGGYQ
                     LAGRYYRPAEGEDLPGVGLLDLWTEHPGPSARRLIGNLVVAVEGMDDPLVGFENHGGR
                     TRLGPNARPLGRVVTGFGNNGEDGWEGAVSRRVYGTYLHGPLLPKNPAFADRLMREAL
                     DRRHPGVTLPPLPDAQAARARAVMLARLGQRAAG"
     gene            complement(6245..7651)
                     /locus_tag="VEW91_01365"
     CDS             complement(6245..7651)
                     /locus_tag="VEW91_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYM90263.1"
                     /translation="MRERDPIGVRLSAAIALGKVTATLSRRLQQGGGTTMPGRVARAV
                     EPRLVPLLSRRLHHGCVIVAGTNGKTTTARLLAHIMRAIGWRPVHNRAGANLAAGIAS
                     AFVEDVDLWGRPAGDVGIFEVDEATVPRVVPHLAPRVAVFTNLFRDQLDRYGEIDHIA
                     GLWRATGKALPAGTVLVANGDDPLVYEAVRGLPGQLVTFGIEDDRHALPTIEHTAEAR
                     YCYRCGTPYQYTLTYFGHMGRYRCATCGVGRPALDVAARDVTLQGPDGAAFLLDAHGR
                     GAVRVATALPGLYNVYNVLAASTAALSLGASLEQVGAAVGSFAPAFGRAERISARGAE
                     LRVLLSKNPAGFNEVLRTVLSAGSPPVLLIAINDNIADGRDVSWLWDVDFERLAGRVA
                     HVVVAGLRAEDMALRLRYAGLPDAAVEVQPEYEPALRSALNRAAGQPLFVLPTYTAML
                     GIRDVLRRWGAVGRYWEH"
     gene            7807..7941
                     /locus_tag="VEW91_01370"
                     /pseudo
     CDS             7807..7941
                     /locus_tag="VEW91_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7990..8196
                     /locus_tag="VEW91_01375"
     CDS             7990..8196
                     /locus_tag="VEW91_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HYM90264.1"
                     /translation="MEISARNQLRGRVTAISLGGVMAEVRLQVGGEELVAVITRTSVE
                     RLGIRVGDEVLAVIKATEVMIGKP"
     gene            8266..>8599
                     /gene="modA"
                     /locus_tag="VEW91_01380"
     CDS             8266..>8599
                     /gene="modA"
                     /locus_tag="VEW91_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYM90265.1"
                     /translation="MRPPTAGAGPSPAVTVFAAASLTEAFAALGKILEGRTPGIRVTF
                     NFAGSQQLALQIEQGAGADVFASADQRWMQYVQGRGLVLGAPREFARNRLAIILPHSN
                     PAGITRLQD"
BASE COUNT         1389 a         3059 c         2888 g         1263 t
ORIGIN      
        1 cctcccggtt gagcgtttcg atcgcagacc gcaggctctg ctgggtcagg acgcgcatct
       61 gatggtcggt ccaggccctg ctcacccaca cgaagcccgt ccccacaccg aagatcacga
      121 ccgaggcgat cgtgaggccg accagcagct cggccagcgt gaagcctttg gacgagcggt
      181 tactggagcg gactgaggat tgtcgaggtc gcataactct tccccccacc ccaggtgacc
      241 gtgacagcca cccgcttcat ccctgtctgc ggatcgtcca ccgtgaccgt ggtctgcaca
      301 gtatacgtgg tggcaccgac cgtcggggtg gccgtatacg tcccggccac aagcgagccg
      361 aagttgatgt cacggtactg ctcgatggtc tgggcgccaa ttgcggcggc gtcgctctgc
      421 tgcctggaca acgccacctg gccggtcgcc atcagcacca gctgcgcggt cgccaccagg
      481 cccaccgtga gcaggaaggc cgcgacgatc acttccatga cggtgaaccc acagcaagaa
      541 tctcgcgtgg gtgcaggcct acgcatgcct tcgacctcga cccccctcac ggtctctcgg
      601 tccatagtgt ctgtatgccc atctcggcgc tcgcttagat gggggatccc gcctcaactg
      661 tggtccacca tccccacccc gttcacggtg accgtcttgg tccagttgcg ggcggcgtcc
      721 ttgagcgtga agacgggcag cggcgacgtg acgagcatgc cccgcgtgtc gaactcggcg
      781 atgagtccga gcgtccccgc gtcaaacctg acgttgggcc gcagggtgat cgtccgctcc
      841 gtcacccagg aggggccgct gtttctttcc atcacgtaca cgtagggcgg gccggagctg
      901 aaaacgcgca cccggtagtg ctgggtgcgg ctgatgccga gttcccgggc cacctgcagg
      961 ttattcgaga gatcctgcat gtcggcggcg aggttctggg gcttggcgtt cagcgctggg
     1021 agcgcgatgc gtatgagaat ccccatcagc gcaagaatga tggccatctc caccagggtc
     1081 ttcccgctgg tgccagccag cttgcgcgct ctcacagcat cgccccccag tcaccacggc
     1141 tcccggcacg ctcccggtca tgccgttagt attcaactaa ttgatacccg gccatcggcg
     1201 ggagtagtcc ttaccctggg aagcttttag cgaagaacgt gggaagatct tccgaagttc
     1261 gggggacggc tacaggctcc cgctcgccgc cggaccgccc gggccgtgca ccaggggggc
     1321 gagaaaggga ttggtccgct tctcctcgcc gagtgtcgtc tccggtccgt ggccggggta
     1381 gcagaccgtc tcgtcgggga gcggcagcag cacacgcgag atcgaagcca ggagcgtttc
     1441 caggtcgccc ccgggaaggt cggtccggcc gatgctgccg gcgaacagcg tatccccgac
     1501 gaacgcgagt ccgtccccga gcaggcacac cccgccgggg ctgtgacccg gcgtgtggcg
     1561 caccgtcagg gtcagccgcc cgaccttcag ggtgtcgccc tcgacgagcc accggtccgg
     1621 caccggcgcg ggcggcaggt cgagcccgaa cagcaccttc gcccgggccg gcccctgcgt
     1681 gaggacctcg cgctccgcct cgtggatcca gaacggggcc ccggtcgccc gccgcacggc
     1741 ctccacgccc tggatgtggt cgaagtggcc gtgcgtgttg acgagcgcca cgggccgggc
     1801 gccgagttgc cggagcgtct cgagaatgcg atccggctcg tccccgggat cgatgacgaa
     1861 cacttcgcgg gcagcagggt ccccgaagat gtagcagttg gccccgatcg ggccgacggg
     1921 gaacccgcga aggatcaccg cgcgggggac gatctaggcc ctcgcggtca ccctggcttc
     1981 gctcagcgcc ttgacgatct cccggcagaa cgccggcaga tccggcggcg tccgggatga
     2041 gatcaggttg ccgtccacga ccacttcttt gtcgacgtag tgggccccgg cgttgatcac
     2101 gtcgtccttc accgcgctga cgcaggtcat cgtcctgccc ttgacgatcc ccgccgagat
     2161 cggcacccag gccgcgtggc agatcgccgc cacgaccttg cccgcttgga acgcctcccg
     2221 gacgagccgg agcatcgccg gatgacgccg catccgatcg ggcgcgtagc cgccggggac
     2281 gatcacggca tcgaaatcgg ccgcccgggc atcgtccatc gaaaggtcgg cctttgcggg
     2341 gtagccctct ttgctcttgt acgactcccc cgccttgggg cccacgatcg tcacctgggc
     2401 gcccgcctcc cggagccgca gcaccgggta ccacaactcc aggttctcat agtagtcttc
     2461 ggcgagcacg gcaacctgct tgccagcaag gttcatagcc actcctcccg tcaggtgtgc
     2521 gcgaggcgcg cacttcgtgt ggacagatcc agtatacgtg tcgctgcgac ggggggtcaa
     2581 gcgaatggag ggataggaga cgggcggccg cgccacctgt gattcccata gacattgttg
     2641 acacttcggg ccgcaggagt ctggcctccc agtgccgtgt ggggacgatc atggttgtag
     2701 tggtgcagcc acttcgggag ggcccgcgtg tgcggactgc gccccagcat gcgatggccg
     2761 cctccggcgg ggatgcgggc caagagcctc atgtcgagat ggatgagctc gcccggatgc
     2821 tcgcggacgt agcgaatggg gatccctgtg gtgcggtcgg cgtcccgcag ccgggagagc
     2881 ccctcccggc ggaggatggc gtacacggtg gagcgcgcca ccccgaggag cgggcccaag
     2941 cggtgcgggc ccagtttgcg acggcgccgc agccgctgga ccgtggccgg gggggtgcac
     3001 cgcggagacg tgtgcggtcg agcggaccga tcctgcaagc cctcagtgcc agcgtgctgc
     3061 cagcggcgga gccacttgta ggcggtctcc cgagagacgc ccgcggaggc ggctgccgcg
     3121 ctcacggtcc atccgagctc cataatccgc tgcaccaaca agtagcgccc aaatggggtg
     3181 agcttccgat gcggatgcgc catgagagcc tcccgacccc ggggtacgga catcccgtcc
     3241 ccatcaggtt cgagaggctc tcgagtgtca acaacctatt tgggaactac acctagccgg
     3301 ggtgccggag ctggccgtag acccacagta ctgcgcgcac ataccactgg gtctccgcgt
     3361 acgggggaat tcccccgtag cgtcgcaccg cctgcccgcc ggcgttgtac gcggcgaggg
     3421 cgagcgtcca atctttgaac tccaagtagc tccaccgcag aatctgcgcg gcccccaaga
     3481 cgttttgcca ggggtccttc gggtcgaccc cggcggcctg cgccgtcccg ggcatgagct
     3541 gccccaggcc catcgcgccc gcccagctga tcgactggtg gttgaaccgg ctttcgatgt
     3601 agatcaccga cgcgaggagc cagggatcga ggccttcccg cccggcggcg gcctggacgg
     3661 cctgccccag ccagcgcgcc tgactccatg tcaggcgcgg attactggcg taggcgagcg
     3721 cggcgatccc cgcggccgaa tcgaccgcgc cggggaccgg agcgtcaaac gcctcaagcc
     3781 gcaggagcca cgcctccgcc agtcgggcct catccggcgt atgggcgcgc cgcagcgcct
     3841 cacggagata cgcgcgcgag tcctgccacc gtcccaccgc cgctgccgcg actcccgccc
     3901 acaatgcggg cagcgcgtca tggggagccc gtgccgccgc ctcggcaaac gattgcagcg
     3961 cgtgagcggg ggctccggtg cgatacgcgt tgatccccgt gcggaggagc cgctcagcgt
     4021 ccccggggcc ggcgtgcacg gggccacaga ccgaaagggc gactgccaga gcgagccccg
     4081 cgcatagatg cttcgaccag gcactcctga gcacgctgca gccccccggg cggcccggtt
     4141 cccaccgatc cgccctctgc atcccccgat gatcccctca agagcatgat gcccttaaga
     4201 ggcggggaac cggaccagcc ggtccggggg ccagcgcgtt ccggagttgc tcgaagggtt
     4261 tgtttcgaag ggtttatctt gctggggtgg ggcgcctgcg gcccagcgtg agcagcgcga
     4321 ggcaggtgcc taccgtaagc agtgccgcgc tcgccacgaa cggcgcctgg ggccccacgc
     4381 catcgtagag cacccccccc aggagcggcc caatgacgag cccggccccc tgcgcggtct
     4441 cagacgcccc caggaccatc ccggtgctgc cgcggggcgc cgcctcgctc atcagtgcca
     4501 gccaggcggg agacgagacc gcgtagcttc ctcccaggag cagcgccgcc accccgagca
     4561 cgagcaggtt gccacggccg gcgagcggca gcagccacat cccgacgctc gccagcgcca
     4621 tcccccagac caccgtgcgg ggccggccga cccgatcggc gagccgcccc cccgggaccg
     4681 aggcgatggc gacggccagc accatgacga gaaacatcgt cccgatcaac tgatcgctga
     4741 gctgcacgac ctcgtgggca tagatcacga ggatcggcgc gagcaggccc acgccgaaca
     4801 tctgcacaaa cgccacgagg agcatcgcga gcagcgaggg cgatcgccgg atgccctgga
     4861 aaattccgcg cgcccagact ccgggcggtt ggtggtgggc aggcgcgtgc cgttcgccgt
     4921 cgcgaaacgt gagcgtcgcg agcgcggcgg cgccgagcag gagggcgctc gcgagataga
     4981 acccccacgt gtagccgccg aggaaccccg agacaaacag gccgaggctc ggcccgagcg
     5041 caagccccga gagaaagaac atgttgagcg tgcccatcgc cgtggcgcgc tgctcgtcct
     5101 ccgtccggtc caccatcatc gcggccgccg ccggccagag cgcggcgctc cccatcccat
     5161 cgatcaggcg gagcgggacg aacaaccacg ccgagcgcag cagcgtcatg atcaccggcg
     5221 ccgtgctgct caggaacagc ccccagacga tgacgggccg ccgcccgatg cggtccgcca
     5281 ggctccccca cgtcgtcttg aacacggtct ccaccgcggc gaaggtggcg accaccacgc
     5341 cgacgagcgt ggcgctcgcc ccgaggcgcc ctttgaggta cagcggcagc agggggatca
     5401 cggtcgcaaa cccgagctcc gccatgccga cgatcgcggc gacccgaacc agccctcggc
     5461 tgatgccgcg cgcgccgcct cccacccgac tacccggccg cccgctgccc cagtcgggcc
     5521 agcatcaccg cgcgggcccg tgccgcttgt gcgtccggca gaggcgggag cgtcaccccc
     5581 ggatgccggc gatcgagggc ctcgcgcatg aggcgatcgg cgaacgctgg gttcttcggc
     5641 agcagcgggc cgtgcaggta tgtgccgtac acccgccggg acacggcgcc ttcccaccca
     5701 tcctcgccgt tgttcccaaa cccggtcacc acacgcccca gcggccgcgc gttgggtccg
     5761 agacgcgtcc ggccgccgtg gttctcgaac ccgaccagcg gatcgtccat cccctcgacc
     5821 gcgaccacca ggttgccgat caggcgccgg gcgctggggc cgggatgctc ggtccagagg
     5881 tcgagcaggc ccacgcccgg gaggtcctcg ccctcggcgg ggcggtagta gcggcccgcc
     5941 agctggtatc caccgcagac cgcgagcatg acgccgccgt ccaggacggc atcctcgagt
     6001 gccgcccgct tgacccggca gagatcatcg gccgcgatcc gctgctggcg gtcttctccc
     6061 ccgccgatga agaacagatc cgccgcgtcg gggtccaccg cctcgccaag ccccacctcg
     6121 atcacggtga cgggaatccc tcgccaccgc gcccgctgca tcaggaccat gatgttgccg
     6181 cggtctccgt agagattcag gagatcgggg tacagatggc agatgcgaag ctcggggatc
     6241 cggctcaatg ttcccaatac ctccccaccg ccccccatcg tcggaggacg tctcgaatcc
     6301 ccagcatggc ggtgtacgtc ggcaggacga acagcggctg tcccgcggcg cggttcaacg
     6361 ccgaccgcag cgcgggctcg tactcgggct ggacctcgac cgccgcgtcg ggcagcccgg
     6421 cgtaccgcag gcgcagcgcc atatcctcgg cgcgaagccc ggcgacgacc acgtgcgcga
     6481 cgcgaccggc cagccgttcg aagtcgacat cccacagcca cgagacgtcg cgaccgtccg
     6541 cgatattgtc gttgatggcg atgagcagca ccggtggcga gccggccgac agcacggtcc
     6601 ggagcacctc gttgaacccg gccgggttct tcgagagcag cacccggagc tccgcgccgc
     6661 gcgcgctgat gcgctcggcg cgcccgaacg caggggcgaa cgaccccacc gccgcgccca
     6721 cctgctccag cgaggccccc agggagagcg cggccgtgga ggcggcgagg acgttgtaga
     6781 cattgtagag ccccggcagc gcggtggcca ccctcaccgc cccgcgcccg tgagcatcca
     6841 ggagaaacgc cgctccatcc gggccttgga gggtcacatc gcgggcggcg acgtcgagcg
     6901 cgggacggcc gaccccgcag gtcgcgcacc ggtaccgccc catgtgccca aagtacgtga
     6961 gggtgtactg gtagggggtg ccgcaccggt agcagtaccg ggcttccgcg gtgtgttcga
     7021 tcgttgggag cgcgtgacgg tcgtcctcga tgccgaaggt gacgagctgc cccggcaggc
     7081 cgcggaccgc ctcgtacacc aacgggtcgt cgccgtttgc caccagcacg gtgccggcgg
     7141 ggagcgcctt ccccgtcgcc cgccacaacc cggcgatgtg atcgatctcg ccgtaccggt
     7201 ccagttgatc gcggaacagg ttcgtaaaga cggcgaccct gggcgcgaga tgcggaacga
     7261 cgcggggaac cgtcgcttcg tcaacctcga agatgccgac gtcccccgcc gggcggcccc
     7321 agaggtcgac gtcctccaca aacgccgagg cgatgcctgc ggccaggttt gcgcccgcgc
     7381 gattgtgtac cggcctccaa ccgatggccc gcatgatgtg cgcgagcagc cgggcggttg
     7441 tcgtcttccc gttcgtgccc gcgacgatca cgcagccgtg gtggaggcgc cgggacaaca
     7501 gcgggaccag ccgcggctcg accgctctcg ccacgcggcc cggcatcgtg gtcccgccac
     7561 cctgctgaag gcgccggctg agggtggccg tcaccttccc aagggcgatc gcagcgctga
     7621 gacgcacgcc gatcggatcc cgctcccgca tcgccatgac catcgtacag atgtctgccc
     7681 cagttgaggt tctcgtaggg ggtggcagag gcctgcgata agcaaccttg cagccccagc
     7741 cttgttcagc ctcttatatt cctgtactat actttaaagc cctataaaat ggtagattaa
     7801 ggatatatga gggaatacct gacggccaac gaggctgctt ccctgttgca ccttcacgtg
     7861 aagcgagtgc aggccctggc gcgggcgggg aagctcccag ggacgcgggt ggggcgcaag
     7921 tggttgttcc acaaagaaca actcttcgcc cggctggggc agggcggccg tccgcgggag
     7981 gaggctgagg tggaaatcag cgcacgaaac caactgcggg gcagggtcac ggcgatctcg
     8041 ctgggcggcg tcatggccga ggtgcgcctc caggtcgggg gcgaggaact cgtcgcggtg
     8101 atcacgcgaa cctcggtcga gcggctgggc atccgggtgg gcgacgaggt cctggccgtg
     8161 atcaaagcca ccgaggtcat gatcgggaag ccctgacgcc cgccggccat ggtgccgccc
     8221 cgtacgcgct gcctcgccct tgcggcctgc ttggtctgcc ttgccgtgcg gccgcccacc
     8281 gctggggccg gcccatcgcc cgcggtcacc gtcttcgcgg cggcctcgct gaccgaggcg
     8341 ttcgcggcgc tcggcaagat cctggagggc aggaccccgg ggatccgggt gacgttcaac
     8401 ttcgccggtt cgcagcaact cgcgctgcag atcgagcagg gggcaggggc ggacgtcttc
     8461 gcctcggcgg accagcggtg gatgcagtat gtgcagggac ggggactcgt gctcggcgct
     8521 ccccgggagt tcgccaggaa tcgcctcgcc attatccttc cgcactccaa tccggccggg
     8581 atcacccgtc tgcaggacc
//
LOCUS       DASQGH010000071         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31485, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000071 DASQGH010000000
VERSION     DASQGH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31485"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..823)
                     /locus_tag="VEW91_01385"
     CDS             complement(59..823)
                     /locus_tag="VEW91_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM90266.1"
                     /translation="MQSEVLYEQRGDIVVVTLNRPERRNALSISACHQLRAAWDRFEA
                     DGASKVAIITGAGDRAFCAGYDIAEKQHGNAVSEADFAPRVETRPFVTKPVIAAVNGA
                     AVAAGMALVEACDLVVAAETAWFGLPEVTLGIGVAPFVQSLWTLPQHILMELLLTGDR
                     LAAPRAYEIGFVNRLTPPENLMAESLRLARTIADNAPLVVRASKAMLYRGIEAIGMPA
                     AHRVAKELFLPIRESEDAKEGFRARAERRPPRWKGR"
     gene            complement(844..1707)
                     /locus_tag="VEW91_01390"
     CDS             complement(844..1707)
                     /locus_tag="VEW91_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYM90267.1"
                     /translation="MAVVLGRPGAWTEAFRYTAEAAQIAAYAAATNETDTRFAAGALA
                     SPMYATVPVRPAVRAAFAQAMPEGLPPTVPRLAGEQDMFFHQPIVAGMTVSVRGRFLG
                     IFPKSSGTALVFHFETRSDVDRLLNEQYMTTFLPRVAWPEEAGERPPDHAMPEDIQGR
                     APLARVVQRFDDDQTYRYSAASGDPSRWHIDNEFARAAGLPGIIIHGLCTMAFVARAV
                     AQTAGDGDLLRLKRLAVRFSRPIYPGSEMVTVIWLGHRNGAGTRLFCEAVGADGAKVI
                     TNGLAEVAPPS"
BASE COUNT          280 a          628 c          568 g          279 t
ORIGIN      
        1 ctgcgcgtcc cacggatacc gcgtcaaggt cacgagcgac aggctcagct tcacgtggct
       61 accgcccctt ccaccggggc ggcctccgtt ccgcgcgcgc cctgaacccc tccttggcat
      121 cctcgctttc gcggatcggc aagaagagct ccttggccac ccggtgagcc gccggcatac
      181 cgatggcctc gatgccgcgg tagagcatcg ccttgctggc gcgaacgacg aggggggcgt
      241 tgtcggcaat ggtccgggcg aggcgcagcg actcggccat gaggttctcc ggtggcgtca
      301 ggcgattgac gaacccgatc tcatacgccc gtggcgccgc gagtcggtcg cctgtcagga
      361 gcagctccat caaaatatgc tggggcagcg tccagagcga ctgcacgaac ggggcgaccc
      421 cgatcccgag cgtcacctcc ggcagcccga accacgccgt ctcggcggcc accaccagat
      481 cgcacgcttc taccagagcc atccccgcgg cgaccgccgc gccgttgacg gcggcgatca
      541 cgggcttggt gacgaagggc cgcgtctcca ccctcggcgc gaagtccgct tcgctgacgg
      601 cattgccgtg ctgcttttcg gcgatgtcgt agcctgcgca aaaggcgcga tcgccggcgc
      661 cggtgatgat ggcgaccttg ctcgcgccgt cggcctcaaa ccggtcccac gcggcgcgca
      721 gttggtggca cgctgagatg ctcaacgcgt tccgacgctc cgggcggttg agcgtcacca
      781 cgacgatgtc gccccgctgt tcgtagagga cctcggattg cacgccgctc tctccaacga
      841 gactcatgac ggcggcgcga cctcggccag gccgttcgtg atcaccttgg cgccgtccgc
      901 acccaccgcc tcacagaaga gccgcgtccc ggcaccgttg cggtgcccga gccagatcac
      961 cgtcaccatc tcgctccccg gatagatcgg acgcgagaaa cgcaccgcca gccgcttgag
     1021 cctgaggagg tcaccgtcgc cggcggtttg ggcaacggcg cgggccacga acgccatcgt
     1081 gcagagtccg tggatgatga tccccgggag ccctgccgcc cgcgcgaatt cgttgtcgat
     1141 gtgccagcgg ctgggatccc ccgaggcggc tgaataccgg tacgtctggt catcgtcgaa
     1201 gcgttgcacg accctcgcga gcggggcgcg gccctggatg tcctccggca tcgcatggtc
     1261 gggcgggcgc tccccggctt cctccggcca ggcgaccctg ggcaagaacg tcgtcatgta
     1321 ctgctcgttc agcagtcggt cgacgtccga cctcgtttcg aagtgaaata cgagtgccgt
     1381 cccggacgat ttggggaaga tccccaagaa tcggccccgg acggacacgg tcatgcccgc
     1441 aacgatcggc tgatggaaga acatgtcctg ctctccggcc aggcgcggga ccgtcggagg
     1501 caatccttcc ggcatggcct gggcaaaggc cgcgcgcacc gcgggccgga cggggacggt
     1561 agcgtacatc ggcgaggcca gcgcacccgc cgcgaacctc gtgtccgtct cgttcgtcgc
     1621 cgccgcatat gccgcgatct gggcagcctc ggccgtatac cggaatgcct ccgtccacgc
     1681 ccctgggcgc cccaggacaa cagccatccg cgatctctcc tcagcggtgg gtgtcataaa
     1741 tccgctggcg accga
//
LOCUS       DASQGH010000072         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45654, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000072 DASQGH010000000
VERSION     DASQGH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45654"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VEW91_01395"
     CDS             complement(<1..334)
                     /locus_tag="VEW91_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYM90268.1"
                     /translation="MGRMLIPALAALIPLLPLAGWVANGLWGGRLGRRAVAAVACGAC
                     GAAFAASALLFWQLRALPGEVAHVDLYQWIGVGPLQVPLRLLVDPLSVTMALVVTAVG
                     FLIHVYSAG"
     gene            complement(338..637)
                     /gene="nuoK"
                     /locus_tag="VEW91_01400"
     CDS             complement(338..637)
                     /gene="nuoK"
                     /locus_tag="VEW91_01400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYM90269.1"
                     /translation="MPAPYHLALSAILFTLGMLGVLVRRSALIVFMSIELMLNAVNLA
                     LVTFARVWGSLDGQLLVFFILVVAAVEVVIGLAIIVDLFRSRETVDIDEVDLLRG"
     gene            complement(643..1134)
                     /locus_tag="VEW91_01405"
     CDS             complement(643..1134)
                     /locus_tag="VEW91_01405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYM90270.1"
                     /translation="MGAVLFGIVAGGALAGAAGVIASRRPVYSALALLLVLGSLAVDY
                     LLLDAPFVAVLQVIIYAGAIVVLFVFIIMLLHAHSGEAPRVGAVLPPAAAGVLCAAFG
                     LVVLAVLIRPARAPALLGGQFGTVQEIGRALFSGFLLPFEAASVILLIGMIGAVALGR
                     RLR"
     gene            complement(1135..1623)
                     /gene="nuoI"
                     /locus_tag="VEW91_01410"
     CDS             complement(1135..1623)
                     /gene="nuoI"
                     /locus_tag="VEW91_01410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HYM90271.1"
                     /translation="MSADALGQRLGQTWAMVKGLGIVGRYLFRRPITIQYPEAKPPVA
                     PRFKGRHFLTLFADGMERCVGCELCVIACPSQSIYVRAEENDPLSPHSKGERYAAEFQ
                     INMLRCIFCGLCEEACPTGAIVLGYEYELSGYTRASLIYTKDRLTERVPGESGRDPTR
                     EV"
BASE COUNT          304 a          592 c          557 g          175 t
ORIGIN      
        1 agccggcgga gtagacgtgg atcaggaacc ccaccgcggt gacgacgagg gccatcgtca
       61 ccgagagcgg gtcgacgagc agccggaggg gcacctggag cggccccacc ccgatccact
      121 ggtacaggtc cacgtgcgcc acttctccgg ggagggcgcg cagctgccag aacagcaggg
      181 cggatgcggc gaaggccgcg ccgcaggccc cgcaggcgac ggccgcgacc gcgcggcggc
      241 cgagccgccc cccccacagg ccgttcgcga cccagccggc cagcgggagg agcgggatca
      301 gcgccgcgag ggcggggatc agcatgcggc ccatcgccta cccccggagg aggtcgacct
      361 catcgatgtc caccgtctcc cgggatcgga agaggtccac gatgatcgcc aggccgatca
      421 cgacctcgac ggccgcgacc accaggatga agaacacgag gagctggccg tccaggcttc
      481 cccagacgcg cgcaaacgtc accagcgcca ggttgaccgc gttgagcatc agctcgatcg
      541 acatgaacac gatcagggcc gagcggcgga cgaggacccc gagcatgccc agcgtgaaca
      601 ggatcgcgct gagcgcgagg tgatacggcg caggcacggc catcaccgca gccgcctccc
      661 cagcgccacc gcgccgatca tcccgatgag caggatgacg gacgccgcct cgaacggcag
      721 gaggaagccc gagaacagcg cccggccgat ctcctggacc gtgccgaact gtccgcccag
      781 gagggcgggc gcccgcgcgg ggcggatcag gacggcgagc accaccaggc cgaacgccgc
      841 gcacaagacc cccgcggcgg ccgggggcag caccgcgccg acccgggggg cctctccgct
      901 gtgggcgtgc aggagcatga tgatgaacac gaagaggacg acgatcgccc ccgcgtagat
      961 gatcacctgc aggacggcga cgaagggcgc gtcgagcagc agataatcga ccgcgaggct
     1021 ccccaagacc aggaggagcg cgagcgcgct gtacaccggc cgccgggaag cgatcacccc
     1081 cgccgcaccg gcgagggccc ctcccgcgac gatcccaaac aggacagcgc ccacctacac
     1141 ctcccgggtg ggatcccgcc cggactctcc cggaacgcgc tccgtcaggc ggtctttggt
     1201 gtagatgagg gatgcgcggg tgtaccccga gagttcgtac tcgtagccca gcacgatcgc
     1261 cccggtgggg cacgcctcct cgcacagccc gcagaagatg caccggagca tgttgatctg
     1321 gaactccgcc gcgtaccgct cccccttgga atgggggctc aacggatcgt tctcctccgc
     1381 ccgcacgtag atcgactggc tggggcaggc gatcacgcac aattcgcacc cgacgcaccg
     1441 ctccatgccg tccgcgaaca gcgtgaggaa gtggcgcccc ttgaaccgcg gcgctaccgg
     1501 cggcttcgcc tcggggtact ggatggtgat gggccggcgg aacagatacc ggccgacgat
     1561 gcccagcccc ttgaccatcg cccaggtctg gcccaggcgc tgtccgagcg cgtcggcgct
     1621 caccccgt
//
LOCUS       DASQGH010000073         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14503, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000073 DASQGH010000000
VERSION     DASQGH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14503"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..867)
                     /locus_tag="VEW91_01415"
     CDS             complement(49..867)
                     /locus_tag="VEW91_01415"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYM90272.1"
                     /translation="MKPVLFQVPTPFVTFPVSSFGIFLLLAFVVAILVTQARTRRLGW
                     DSGEVVDLSLYAIIGGIIGARLGYVLGDLRDFVAAPARVLMIWVDAGLAFYGALIGGA
                     LVAWVYGRRRHWRLAQIADAAAPGLALGYAIAMIGALLYGLNYGRPAHLPWAVMLFGE
                     PRHPTQLYLMVAALVVFAIVLAADRVPHPSGRLFWLFVLLMAVARIGIDVFMDSPRLF
                     GGLTLAQVASLVAGVLAAAMLLVRGRGAPAGEAPAGSGSPAEPIAPSGAGGPNG"
     gene            complement(872..1435)
                     /gene="lspA"
                     /locus_tag="VEW91_01420"
     CDS             complement(872..1435)
                     /gene="lspA"
                     /locus_tag="VEW91_01420"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYM90273.1"
                     /translation="MRQTDRRQTSPGDPLRAALHQVQGTGGAPPHRPLTAIPWAFCAA
                     GLAGLAASQAAGGAVARLVPVGGERVLIPRVLSLVVVHNQGIAFGLLPRLSPTAAVVL
                     ALTVLAILFYNRGAWPAASATQWGVGLIFGGALGNIVDRLRFGYVVDYLDVHVWPVFN
                     LADAAIVAGAGVLTAAAFVRRRREVRG"
     gene            complement(1333..1842)
                     /locus_tag="VEW91_01425"
     CDS             complement(1333..1842)
                     /locus_tag="VEW91_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYM90274.1"
                     /translation="MPAKRSRKPIPSRARTSRSRVVGRGGAPSSNGSRGRGRSGSPRG
                     AAGRVEEFTRLLLEERKRLREELSEMEEHQVKAEEKPVADAAGYEEDLADVATETFER
                     EKGLALASSVQGMLAMVEEALQKVRHGTYGVCEGCGKPIDGKRLRAIPYARLCIKCKE
                     REERHRIAR"
     gene            complement(1843..2700)
                     /locus_tag="VEW91_01430"
     CDS             complement(1843..2700)
                     /locus_tag="VEW91_01430"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HYM90275.1"
                     /translation="MDRARGRRPRARRKPAPGAGAEKVLGVLGGMGPLATADFYVKLV
                     RLTPARTDQDHPRVIIDSNPKIPDRTAGLEGEGPDPTPSLTATARALERAGVDVIAIP
                     CNSAHAYLAAIRKAVRVRVLDIMEEVAATAASLTPRPRAVGLLATPGTLRARLYHRAL
                     AAQGIEVVAPTADEEASVEGAIKAVKAGNLGAETRARVREAAAALIRRGADAVVLGCT
                     ELPLVMGPRDAPVPVLDGTEILAAAALREALPAHRGRFKEFSLPRRRSFSAVSLKHHL
                     GAPQPARRR"
     gene            complement(2712..>5524)
                     /locus_tag="VEW91_01435"
     CDS             complement(2712..>5524)
                     /locus_tag="VEW91_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYM90276.1"
                     /translation="FGIAAFVERCKARVLQYAGVQTQQSLRLGYWMDWEHSYYTMSDE
                     NNYCIWLFLKRCWERGLLYKGHDVMPWCPRCSTGLSEHEIVTEGYQEVTHPGLTVRLP
                     LRDRPGAYLLVWTTTPWTLTSNVAVAVNPHLTYVRVEQGRDTYYLLRDRLEMLRGHAR
                     VREELPGSAMVGWGYTGPYDDLPAQQGVEHRIIPWGEVSATEGTGLVHIAPGCGAEDF
                     ALGKEHALPVLAPIDEFGIFTDRFGWLVGQHVYEVAGPIREDLERRGLLYRADDYTHR
                     YPMCWRCGSELVFRLVDEWFIRMDPLRAPMAAVTEQIRWIPEFGLERELDWLRNMHDW
                     MISKKRYWGLALPIWECRACGSFEVIGSEHELRERAVEGWEAFQGHTPPRPGIDAIKI
                     RCRTCGAPVSRILDVGNPWLDAGIVPYSTLGYRHDPAAWRGWFPADFITEAFPGQYRN
                     WFYSMLVMSTVLENREPYRVCLGHAMVRDEQGREMHKSWGNMIEFNEAAEKMGSDVVR
                     WLYAVQNPSQNINFGYGPADDVRRRFVLPLWNVYAFFVTYATLDRFAPGAPGRMGTPA
                     ALDRWVRALLTRMIGTVRASLDAYDVPGAARAIERFVEDLSLWYVRRGRRRYWKSELD
                     ADKQAAYQTLYDVLVTLCRLLAPFVPFLAEAMYQNLVRAVSPTAPESVHLCEMPVAEP
                     SADDPGLIEATERTRELVSLGRSARNAAKVRVRQPLRAAIVIDRSGVVERYPELAEHM
                     RDELNVKALRFAESYRTLGQLEVRPRFDLLGPKFGGRITAIVEALRERGAALVDETPP
                     REPFRLRLGGGDEVVLERAEVDVRVHWRPGLTGAGESGVWVVLETTLTDDLVREGMAR
                     ELIHQIQQLRKEAGFQIADRITLYYEGDSALAEILRTHRDYVLREVLGQEARAGLPPG
                     AIHRKALRLDGHEFTVGIAQIR"
BASE COUNT          867 a         2032 c         1865 g          760 t
ORIGIN      
        1 cgggcggcgg ccgccgcctc aaaggtcaca gcgcccggtt cgccgcgctc agccgttggg
       61 cccaccggcg cccgacgggg cgatcggttc ggccggggat cctgagccgg ccggggcctc
      121 ccccgccgga gcgccgcggc ctctcacgag cagcatcgcc gccgccagca caccggccac
      181 gaggctcgcg acctgtgcca gcgtgagccc gccgaagagg cgcgggctat ccatgaacac
      241 gtcgatgccg atccgggcga ccgccatgag gagcacgaac agccagaaca ggcgcccgga
      301 cgggtgcggg acacggtcgg cggccagcac gatcgcgaag acgacgagcg cagcgaccat
      361 caagtacagt tgcgtggggt gccggggctc gccgaacagc atgacggccc agggaaggtg
      421 cgccgggcgc ccgtagttga gcccgtacag gagcgccccg atcatcgcga tcgcgtagcc
      481 cagcgcgagc ccgggcgcgg ctgcatcggc gatctgggcg agccgccagt gacgccgccg
      541 gccgtacacc caggccacca gggccccgcc gatcagggcg ccgtagaagg cgagcccggc
      601 atcgacccag atcatcagga cccgagccgg agcggcgacg aaatccctga ggtcccccag
      661 cacgtacccg agccgggcgc cgatgatccc gccgatgatc gcatagaggg acaggtcgac
      721 cacttcgccg gaatcccacc ccaagcgccg ggttcgcgcc tgggtcacga ggatggccac
      781 gacgaacgcc agcaagagga agatgccgaa cgacgacacg gggaacgtca cgaacggggt
      841 tgggacctgg aacagcacag gcttcacctg ctcacccccg tacctcccgg cgccggcgga
      901 cgaaggcggc ggcggtcaac acgccggccc ccgcgacgat cgcggcgtca gccagattga
      961 acacgggcca aacgtggacg tcgaggtagt ccaccacata accgaagcga aggcggtcca
     1021 cgatgtttcc gagcgctccg ccgaagatga gcccgacgcc ccactgcgtg gcggaagccg
     1081 ccggccacgc gcccctattg tagaagagga tcgcgagcac ggtcaaggca agcaccaccg
     1141 cggcggtggg tgagagccgg gggagcagtc cgaatgcgat gccctgattg tggacgacca
     1201 caagcgacag cacgcgcggg atcagcaccc gctctccgcc cacaggcaca agcctcgcca
     1261 ccgccccccc tgccgcctgg ctcgcggcga gccctgccag ccccgccgcg cagaaggccc
     1321 aggggatcgc ggtcagcggg cgatgcggtg gcgctcctcc cgttccttgc acttgatgca
     1381 gagccgcgcg taggggatcg cccggagacg tttgccgtcg atcggtttgc cgcatccttc
     1441 gcagaccccg taggtgccgt gccgaacctt ttggagggcc tcctcgacca tcgcgagcat
     1501 gccctgcacg ctgctcgcga gggcgagtcc tttctctcgc tcaaaggtct cggtcgcgac
     1561 gtccgcgagg tcctcctcgt atcccgccgc atcggcgacc ggcttctcct cggccttgac
     1621 ctggtgctcc tccatctcgg agagctcttc tcggagccgc ttccgctcct ccagcagcag
     1681 ccgggtgaac tcctcaaccc gcccggcggc gccgcggggg gatccggacc gtccgcgacc
     1741 ccgggaccca ttcgacgagg gcgcgccccc ccgccccacg acccgggacc gcgacgtccg
     1801 tgcacgcgaa ggtataggct ttcgagaccg cttggctggc atctaccgcc tccttgcagg
     1861 ctgtggggcg cccaagtggt gcttgagaga caccgcggag aacgaacgcc gccgcggaag
     1921 agaaaattcc ttgaaccggc cccgatgcgc ggggagcgcc tcgcgcaatg cggccgccgc
     1981 cagaatctcg gtcccgtcga gcacggggac cggggcatcg cgcggaccca tcaccagcgg
     2041 cagctccgtg cacccgagca cgaccgcgtc cgctccccgg cgaatcaacg ccgcggcggc
     2101 ctcgcggacg cgcgcccgcg tctccgcgcc gagattcccg gccttcaccg ctttgatcgc
     2161 cccctccacg ctcgcctctt cgtccgcggt gggcgccacc acctcgatcc cctgcgcggc
     2221 gagcgcgcga tggtacagcc gcgcgcggag ggtgcccggg gtcgcgagca ggcccaccgc
     2281 ccgcggccgc ggggtgagcg aggcggccgt ggcggcgacc tcctccatga tgtccaggac
     2341 gcggacccgc accgccttcc gaatcgcggc caggtaggcg tgggcggagt tgcacgggat
     2401 ggcgatcacg tccaccccgg cccgctcgag ggcgcgggcg gtggcggtga gggacggggt
     2461 cgggtcaggc ccctccccct cgagcccggc ggtgcggtcg gggatcttcg ggttgctgtc
     2521 gatgatcacg cgcgggtgat cctgatcggt gcgcgccggc gtgagccgca ccagcttgac
     2581 gtagaaatct gcggtcgcca gcggccccat gccgcccagc acccccagga ccttttcggc
     2641 ccccgcgccc ggcgccggct tccgccgcgc ccgcgggcgg cgcccccgcg ctcgatccat
     2701 cgcgcgtctc ctcatctgat ctgcgcgatc ccgaccgtga actcgtgccc gtccaatcgc
     2761 agcgccttgc gatggatcgc gcccgggggg agcccggccc gggcctcctg gcccagcacc
     2821 tcgcgcagca cgtagtcgcg gtgggtgcgg aggatttccg caagggcgga gtccccctcg
     2881 tagtacagcg tgatccggtc ggcgatctgg aagcccgcct ccttgcgcaa ctgctggatc
     2941 tggtggatga gctcgcgggc catcccttcg cgcacgaggt cgtcggtgag cgtcgtctcc
     3001 agcaccaccc acaccccgct ctcgccggcc ccggtcaggc ccggccgcca gtggacccgc
     3061 acatccacct ccgcccgctc gagcaccacc tcatcgccgc ccccaagccg cagccggaac
     3121 ggttcgcgcg gcggggtctc gtccacgagg gccgcgccgc gctcccgcag cgcttccacg
     3181 atcgcggtga tgcggccgcc gaacttgggg cccaggagat cgaaccgggg gcggacctcc
     3241 agctggccca gcgtgcgata ggattccgca aatcgcaggg ctttgacgtt cagctcgtcc
     3301 cgcatgtgtt cggcgagttc ggggtaccgc tcgaccactc ccgaccggtc gatcacgatc
     3361 gccgcccgga gcggctgccg gacccggacc ttggcggcgt tgcgggccga gcggccgagc
     3421 gagaccagtt cgcgggtgcg ctcggtggcc tcgatcagcc cgggatcgtc cgcgctcggt
     3481 tcggccacgg gcatctcgca caggtggacg ctctcgggag cggtcggcga caccgcacgg
     3541 acgaggtttt gatacatcgc ctcggcgaga aacggcacga acggggcgag cagccggcag
     3601 agcgtcacca ggacgtcgta gagcgtctga tacgcggcct gcttatcggc atccagttcc
     3661 gacttccagt agcggcgccg accgcggcgg acgtaccata aggacaggtc ctcgacgaag
     3721 cgctcgatcg cgcgggccgc cccggggacg tcgtacgcgt cgagcgacgc gcggacggtg
     3781 ccgatcatcc gggtgagaag ggcgcgcacc catcggtcca gggccgccgg cgtccccatc
     3841 cggccggggg cgcccggggc gaaccggtcg agggtcgcgt aggtgacgaa gaacgcatag
     3901 acgttccaca ggggcaggac gaaccggcgg cggacgtcgt ccgccgggcc gtagccgaag
     3961 ttgatgttct gggaaggatt ctggacggcg tagagccagc ggacgacatc gctgcccatc
     4021 ttctccgccg cctcgttgaa ctcgatcatg ttcccccagg acttgtgcat ctcccggccc
     4081 tgctcgtccc ggaccatcgc gtggccgagg cacacccggt acggctcccg gttctccagg
     4141 acggtgctca tcaccagcat cgagtagaac cagttccggt actggccggg gaacgcctcg
     4201 gtgatgaaat cggccgggaa ccacccccgc cacgccgccg gatcgtggcg gtacccgagc
     4261 gtcgagtagg ggacgatgcc ggcgtcgagc cacgggttcc cgacgtcgag gattcgggag
     4321 acgggcgcgc cgcacgtgcg gcaccggatc ttgatcgcgt cgatccccgg ccgggggggg
     4381 gtgtggccct gaaacgcctc ccacccttcc acggcccgct cgcggagctc gtgctcgctg
     4441 ccgatcacct cgaacgagcc gcacgcccgg cactcccaga tcggcaacgc cagcccccag
     4501 taccgcttct tgctgatcat ccagtcgtgc atgttgcgca gccagtcgag ctcgcgctcc
     4561 aacccgaact cgggaatcca tcggatctgc tcggtgaccg ccgccatggg ggcgcggagc
     4621 ggatccatgc gaatgaacca ttcgtcgacg aggcggaaga ccagctcgct cccgcaccgc
     4681 caacacatgg gatagcggtg cgtgtagtcg tccgcccggt acaggaggcc gcggcgctcg
     4741 agatcctccc ggatcggccc ggccacctcg tagacgtgct gccccacgag ccacccgaag
     4801 cgatcggtga agatgccgaa ctcgtcgatc ggcgccagga ccgggagcgc gtgctccttg
     4861 ccgagggcga agtcctccgc cccgcacccg ggggcgatgt ggacgagccc ggtgccctcc
     4921 gtcgcgctca cctcgcccca cgggatgatg cggtgctcca ccccctgctg ggcgggcaga
     4981 tcgtcgtacg gcccggtgta cccccacccc accatcgcgg agccgggaag ttcctcccgg
     5041 acccgggcgt ggccccgcag catctccagg cggtcgcgca acaggtagta ggtgtcccgt
     5101 ccctgctcga cccggacata ggtcagatgc gggttgaccg ccacggccac gttgctggtc
     5161 agggtccagg gcgtcgtcgt ccacacgagc aggtacgcgc ccgggcggtc ccgcagcggc
     5221 agccgcacgg tcaggccggg gtgggtgacc tcctggtacc cttcggtgac gatctcgtgc
     5281 tcggagagcc cggtgctaca ccgcgggcac cacggcatga cgtcgtgtcc cttatacagc
     5341 aggccccgct cccagcaccg tttgagaaac agccagatgc agtagttgtt ctcatcggac
     5401 atcgtgtagt aggaatgctc ccagtccatc cagtacccca gccgcagcga ctgctgggtc
     5461 tgcacgccgg cgtactggag cacccgcgcc ttgcaccgtt cgacgaatgc ggcgatgccg
     5521 aagc
//
LOCUS       DASQGH010000074         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23028, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000074 DASQGH010000000
VERSION     DASQGH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23028"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..553
                     /gene="secE"
                     /locus_tag="VEW91_01440"
     CDS             236..553
                     /gene="secE"
                     /locus_tag="VEW91_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HYM90277.1"
                     /translation="MARVTDGARESALGKPLSAPTGPQRVERPAPRPARFQIRPLPRV
                     WTTTAQFLRDVRAEMDRVAWPDRQTVIASTVVVVFVLVVTAMYLAGWDYIFAGLLSYL
                     FKR"
     gene            802..1371
                     /gene="nusG"
                     /locus_tag="VEW91_01445"
     CDS             802..1371
                     /gene="nusG"
                     /locus_tag="VEW91_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HYM90278.1"
                     /translation="MVEAPPVPGEQAAAADGRPWFVIHTYSGYENKVRTNLERRVASM
                     AMQHKIFRILVPTEDEIEVKDGKRRIAKKKVFPGYVLVEMLMDDDSWYVVRNTPGVTG
                     FVGSGNKPLPLQDKEVKAILRGLGDETPKFRITYQKGSSVRINSGPFMDFSGIVDEIL
                     PEKEKVRVLVSIFGRETPVELDFAQVEKI"
     gene            1396..1821
                     /gene="rplK"
                     /locus_tag="VEW91_01450"
     CDS             1396..1821
                     /gene="rplK"
                     /locus_tag="VEW91_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HYM90279.1"
                     /translation="MKKIVAIVKLQIPAGKATPAPPVGPALGQHGVNIMEFCKSYNEK
                     TARQVGTIVPVEITVFADRTFTFVTKTPPASVLLKKAAGIEKGSGEPNKKKLGKVTRR
                     QIREIAEVKLADLNTRDLEAAMRMIEGTARSMGIEVEVG"
BASE COUNT          352 a          631 c          722 g          292 t
ORIGIN      
        1 ggtttcggct cgcgatcgtc aactgctggc gcctcgggac gggggtcgcc gttcgtgcgc
       61 gcctccggag tctcttcacc gtgatcagcc tcaatggccg cgtcccgcgc caggggagcc
      121 gggggcccgg gagccccctc ccggccgccc ggcgttgaca gccggggcgg cgctggtata
      181 atgaaatgcg gcaagaagtg tcgcaagatg tctttttttg tttgggggaa agttgatggc
      241 gcgcgtcacc gacggggcca gggaatccgc gctggggaag ccgctgtctg cgcccaccgg
      301 gccgcagcgg gtggagcggc cggccccccg gccggcgagg tttcagattc gcccgctccc
      361 gcgcgtgtgg accacgaccg cgcagttcct gcgcgatgtg cgggcggaga tggatcgcgt
      421 cgcgtggccg gatcgccaga cggtcatcgc ctcgacggtg gtcgtggtgt tcgtcctcgt
      481 cgtgaccgcg atgtacctgg ccgggtggga ctacatcttc gcggggttgc tctcgtacct
      541 ctttaagcgg tagacgccat ggcagacgag cgcacccgac cccagggaaa acccgacacg
      601 gccgccctga tccagaaact gtttcgggac gagccggagg aaccccaacc cgccggcgcc
      661 cccgaggagg gcgggccggg accggcgtcc tccgtcgggg tcgtgacgcc gccggaggcc
      721 ggtgaggcga tcccggaggc gcgcggcgaa gaagcgcccc cggaggcccc cgcggaggcc
      781 gcgcccgctt ctgccgaagg ggtggtcgag gcgccccccg tcccgggaga gcaggccgcg
      841 gccgcagacg gccgcccctg gttcgtcatc cacacctact cggggtacga gaacaaggtc
      901 cggaccaacc tggagcgccg ggtggcctcc atggcgatgc agcacaagat cttccgcatc
      961 ctggtcccga ccgaggatga gatcgaggtc aaggacggga agcggcggat cgccaagaag
     1021 aaggtcttcc ccggctacgt gctcgtcgag atgctcatgg acgacgactc ctggtatgtg
     1081 gtgcgcaaca ccccgggggt cacggggttc gtgggctcgg gcaacaagcc gctgccgctg
     1141 caggataagg aggtcaaagc catcctccgg gggctcgggg acgagacccc caagttccgc
     1201 atcacctacc agaagggctc ctcggtgcgg atcaactccg gcccgttcat ggatttcagc
     1261 gggatcgttg acgagattct gcccgagaag gaaaaggtgc gcgtgctggt ctcgatcttc
     1321 gggcgggaga ccccggtgga gctggacttt gcgcaggtgg agaagatcta gcgctcgcgc
     1381 actggcggag gggccatgaa gaagattgtg gcgatcgtca agctgcaaat cccggccggc
     1441 aaggccaccc ccgcgccgcc ggtcggaccg gccctgggcc agcacggcgt caacatcatg
     1501 gagttctgca agtcgtacaa cgagaagacc gcgaggcagg tcggcacgat cgtgccggtg
     1561 gagatcacgg tcttcgcgga tcgcacgttc acgttcgtga cgaagacgcc ccccgcgtcc
     1621 gtgctcctga agaaggcggc cggcatcgag aaaggctccg gcgagccgaa caagaagaag
     1681 ctgggcaagg tgacccgtcg ccagatccgg gagatcgccg aggtgaagct cgccgacctg
     1741 aatacccggg acctcgaggc cgcgatgcgg atgatcgagg gaaccgcgcg gagcatgggg
     1801 atcgaggtcg aggtcgggtg acccggccgg agtgaacggg aggcggacgc catggcgaag
     1861 cccggcaagc gataccgaga agcggccaaa ctggtcaccc cggggcagtt gtacgatccc
     1921 cagacggccg tgtcagtgct caagcggatg ccgaatgcga agttcgacca gaccgtggag
     1981 gtcagcatcc ggctcgg
//
LOCUS       DASQGH010000075         4979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5993, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000075 DASQGH010000000
VERSION     DASQGH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5993"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VEW91_01455"
     CDS             <1..257
                     /locus_tag="VEW91_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90280.1"
                     /translation="ALRNALIPVITVVGFQAGYLLGGTVVIEDVFALPGMGRFALAAI
                     SQRDYPVVQGVVLLIALLFVLTNVVVDLLYAYVDPRIRYG"
     gene            254..1129
                     /locus_tag="VEW91_01460"
     CDS             254..1129
                     /locus_tag="VEW91_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90281.1"
                     /translation="MSRSPAAPRSASPRHRASMATQLLRTPVAAAGAVIVGVYLLAAA
                     AAPVLSPHDPLAMISQSLLAPPGGPYPFGTDQFGRDLLSRLLYGSRVSFTVAFASVGL
                     ALAGGGAAGLAGGYYGGPVDGVLMRVMDVIFAFPAVLLAIAIMAVAGTAVWTVILAIG
                     VVYTPQFARVSRASVLATRNLEYVEAAAAMGAGARRVLLRHILPNISAPLIVQTSLSL
                     SLAILTESALSFLGLGTQPPTPSWGSMLADSRRFMTMAPWTAVFPGVAIAMAVLGFNL
                     LGDGLRDLLDPRLRV"
     gene            1161..2072
                     /gene="murQ"
                     /locus_tag="VEW91_01465"
     CDS             1161..2072
                     /gene="murQ"
                     /locus_tag="VEW91_01465"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HYM90282.1"
                     /translation="MPGREPSTEAINERTRDFDLRPLLDQARLMNAEDQSVPAAVEAV
                     LPQIVDAVTRIAQALRRGGRLLYVGAGTSGRVAALDAAECPPTFGTDPDQVQAVLAGG
                     PRALTEGVEGAEDDERAGEREMDARAAGPKDVIVGIAASGTTPFVLAAVRRARARGAT
                     TIAVTCVADSPLASACDLAIVPLVGPEVIAGSTRLKAGTAQKLVLNMLSTLAMVQLGK
                     VYGNLMVDLRATNEKLRRRAVRIVAAAAGVAEPHAADALARAGGRVPVAIVMLAASTT
                     AEEAAARLARAQGSIRRAVAGADRGTP"
     gene            2098..3315
                     /locus_tag="VEW91_01470"
     CDS             2098..3315
                     /locus_tag="VEW91_01470"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HYM90283.1"
                     /translation="MTLDPFARARAQRTKLVIGLISGTSADGVTGALVSLTDRDGHPP
                     EIVLRGWATIPYPADLRRRLLELFTEPAPIQDVATLHFALGELFAEAAGAAARAAGCD
                     LAAVDLIASHGQTVAHVGTPDPADPRSRAATLQLGEAAVIAERTGRPVIADFRPADVA
                     AGGVGAPFVPYADLLLLGGPIGRIALNLGGISNITVLPAGAGREDVYAFDCGPANMLI
                     DGLVRHFSGGAEAYDRDGARAARGRVRRDLLGELMRHPFVLTPPPKAAGHEQFGRPFL
                     RTLLTTWGSLPADDTIATATAFAVEAIALNIAKFVLPHHAIEEIVASGGGVHNPTLMR
                     HLAAAAPLRVRRIDEFGIPSDAKEALAFALLGHASLMGRAGNLPRVTGARHGAVLGKF
                     TWPPGSAAEDTPR"
     gene            3312..4430
                     /locus_tag="VEW91_01475"
     CDS             3312..4430
                     /locus_tag="VEW91_01475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYM90284.1"
                     /translation="MSGARESGEPAPRALAAVRRLLEDGVGRAYPGAVLGVLHRGETV
                     IRWAVGVRSLLPARQPATPETVYDLASLTKVVVTTPLILQAAAEGRLRPDDPVAAYLP
                     EIAAPGITLRHLLAHSSGLPAWIPFYLEATGYDAVVARAAQTPAASAPGAQVVYSDLG
                     FIFLGEVARRAHGATLDLLARRRIFDPLGMPDTTYLPPPHLRDRIAPTEDGTAIEQAM
                     AGEAGRRHTWRRYLIWGEVHDSNAYAMGGVAGHAGVFGTADDLLGYARMWLAGGRTPR
                     GEVLPPDLVREATTGQAPARTRGLGWALSGPQGWWGESLSPRAYGHTGFTGTSMVIDP
                     EHDLAIVLLTNAIHLGRDRPEILALRPQLVAAVAHALL"
     gene            4497..>4979
                     /locus_tag="VEW91_01480"
     CDS             4497..>4979
                     /locus_tag="VEW91_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HYM90285.1"
                     /translation="MAYFLGVDGGATGTTCAVCDHDGRILGIGRGGPSNHILAPGGEV
                     RARSAVETALGAAEAASGLGRVEFESVQFGMTGINRDTEQARVFMRVIANVLSARIVQ
                     IDNDASVALAGALACRPGVVLIAGTGSVAFGRDPSGQEARAGGWGYIFGDEGSGFAIG
                     L"
BASE COUNT          634 a         1827 c         1805 g          713 t
ORIGIN      
        1 acgcgctgcg aaacgccctg atccccgtga tcaccgtcgt cgggttccag gccgggtatc
       61 tgctcggggg cacggtggtc atcgaggacg tgttcgcgct cccggggatg ggacggttcg
      121 ccctggccgc tatcagccag cgcgattacc ccgtggtcca gggggtggtg ctcctcattg
      181 cgctcctgtt cgtcctgacc aacgtggtcg tcgacctcct ctacgcctac gtcgacccga
      241 ggatccggta cggatgagtc ggtccccggc cgcgccgcgc tcggcgagcc cccgacaccg
      301 ggcctcgatg gcgacccagc tcctccggac cccggtcgcg gcggccgggg cggtgatcgt
      361 gggcgtctac ctgcttgccg ccgccgccgc cccggtgctc tcgccccacg accctctcgc
      421 gatgatctcc cagtccctgc tggcaccccc cggaggaccc tatcccttcg ggaccgacca
      481 attcggcagg gatctgttga gccggcttct ctacggatcg cgggtgtcgt ttacggtcgc
      541 gttcgcatcg gtgggcctgg ccctggcggg tggaggagcg gccgggctcg caggcggata
      601 ctacggcggg ccggtcgatg gggtcctgat gcgcgtgatg gacgtcatct tcgcgttccc
      661 cgccgtgctg ctggcgatcg ccatcatggc ggtggcgggg accgcggtct ggacggtgat
      721 cctggccatc ggagtggtgt acacgccgca gttcgcgcgg gtgagccggg ccagtgtgct
      781 cgcgacgcgg aacctggagt acgtggaggc ggccgcggcc atgggggcgg gggcccgccg
      841 ggtcctcctg cgccacatcc ttccaaacat ctcggcgccc ctgatcgtgc agacctcgct
      901 cagcctctcc ctggccatcc tgaccgagtc ggcgctcagc ttcctggggc tggggacgca
      961 accgcccacg ccctcgtggg ggagcatgct ggccgactcc cgccggttca tgacgatggc
     1021 tccctggacc gccgtcttcc ccggcgtagc gatcgcgatg gcggtgctgg ggtttaacct
     1081 gctcggggac gggttgcgcg atctactgga tccgcgcctc cgagtgtgag gcggcgagcg
     1141 tgaagacgag gaggtgagtg atgcccgggc gtgaaccgtc caccgaggcg atcaacgagc
     1201 gcacgcggga tttcgacctc cggccgctgc tcgaccaggc ccgcctgatg aacgcggaag
     1261 accagagcgt cccggccgcg gtggaggcgg tcctcccgca gatcgtagac gccgtgaccc
     1321 ggatcgctca ggcgctgcgc cgcggaggac ggctgctgta cgtcggagcc gggaccagcg
     1381 gtcgggtcgc ggcgctcgac gccgcagagt gcccgccgac gttcgggacc gatccagacc
     1441 aggtgcaggc cgtcctcgcc ggcggcccgc gggcgctcac cgagggcgtc gaaggcgccg
     1501 aggacgacga gcgggccggc gagcgggaga tggatgcgcg cgccgccggc ccgaaggacg
     1561 tgatcgtcgg catcgccgcc agcggcacaa ccccgttcgt cctggccgcc gtgcgccgcg
     1621 cgcgggcgcg aggggcgacc accatcgccg tgacgtgcgt cgccgactcc ccgctcgcat
     1681 ccgcgtgcga cctggcgatc gtccccctag tcgggccgga ggtcatcgcc gggagcaccc
     1741 ggctcaaagc cgggaccgcc cagaagctcg tgctgaacat gctgagcacc ctggccatgg
     1801 tgcagctcgg caaagtgtat ggcaacctga tggtggacct gcgcgcgacg aacgaaaaac
     1861 tgcgccgccg cgcggtcagg atcgtcgcgg ccgcagcggg cgtcgcagag ccccacgcgg
     1921 cggatgcgct ggcccgcgcg ggaggccgcg tgcccgtggc gatcgtgatg ctcgccgcca
     1981 gcacgaccgc cgaggaggcg gcggcccgcc tggcccgcgc gcaggggagc atccggcgcg
     2041 ccgtggcggg cgccgatcgg ggaaccccgt gaccaaaccc ccggtgaccg tgcgcccgtg
     2101 accctcgatc cgttcgcccg cgcgcgcgcc cagcggacca agctggtgat cggactcatc
     2161 tccggcacct ccgcggacgg cgtgaccggc gccctcgtca gcctcaccga ccgggacggc
     2221 catcccccgg agatcgtcct ccgggggtgg gccacgatcc cgtaccctgc cgatctgcgg
     2281 cgccgcctgc tggagctctt caccgaacct gcgccgatcc aggacgttgc cacgctgcac
     2341 tttgcgctcg gggagctctt cgccgaggcg gccggggccg ccgcccgcgc cgccgggtgc
     2401 gatctcgccg cggtggacct catcgcgtcg cacgggcaga ccgtcgcgca cgtggggacg
     2461 ccggatccgg cggatccgcg cagccgcgcc gcgaccctgc aactcggaga ggcggcggtg
     2521 atcgccgagc gcaccgggcg cccggtcatc gcagactttc gtccggccga tgtcgcggcc
     2581 ggcggggtcg gggccccctt cgtcccctac gcggatctgc tgctgctcgg cggccccatc
     2641 ggccggatcg cgctcaacct cggcgggatc tcgaacatca ccgtgctccc ggcgggggcg
     2701 ggccgcgagg acgtctacgc cttcgactgc ggccccgcca atatgctcat cgacggcctt
     2761 gtccgccact tctccggggg agcggaggcg tacgatcggg acggggcgcg cgccgcccgc
     2821 ggccgggtgc gtcgggatct ccttggggag ttgatgcgcc accccttcgt gctgacccct
     2881 ccccccaagg cggccgggca cgagcagttc ggccgtccgt tcctgcgtac gctgctcacc
     2941 acctggggat cgcttcccgc cgacgacacg atcgccaccg ccacggcgtt tgcggtcgag
     3001 gcgatcgcgc tgaacatcgc caaattcgtc ctgccccacc acgcgatcga ggagatcgtc
     3061 gcctcgggcg ggggcgtgca caaccccaca ctgatgcgcc acctcgcggc ggcggcgccc
     3121 ctccgggtca ggcggatcga tgagttcggc atcccgtccg acgcgaagga ggcgctcgcg
     3181 tttgcgctcc tggggcacgc gagcctgatg ggacgcgcgg gcaacctccc ccgggtgacc
     3241 ggcgcgcgcc acggagccgt gctgggaaag tttacgtggc cgccgggctc ggcggccgag
     3301 gacacgcccc ggtgagcgga gcgcgtgagt ccggtgaacc agccccccgc gcgctcgcgg
     3361 cggtccgccg cctgcttgag gacggggtcg gccgtgccta cccgggcgcc gtcctggggg
     3421 tgctgcaccg cggcgaaacc gtgatccggt gggcggtcgg ggttcgctcc ctcctccccg
     3481 cccggcagcc cgcgaccccc gagaccgtgt acgaccttgc ctcgctcacc aaggtcgtgg
     3541 tcacgacgcc gctgatcctc caggcggccg cggagggccg cctgcggccc gatgacccgg
     3601 tggccgccta tctgcccgag atcgcggcac ccgggatcac cctgcggcac ctgctcgccc
     3661 actcgagcgg gctgccggcc tggatcccgt tctacctgga ggcgaccgga tacgatgccg
     3721 tggtcgcgcg cgccgcccaa acccccgccg cgtccgcgcc gggcgcgcag gtggtctaca
     3781 gcgacctggg gttcatcttc ctcggcgagg tggcgcgccg cgcgcacggc gcgaccctcg
     3841 acctcctggc gcggaggcgg atcttcgacc cgcttgggat gccggacacg acgtaccttc
     3901 cccctccgca cctgcgcgac cgcatcgcgc cgaccgaaga cggcaccgcg atcgagcagg
     3961 cgatggccgg cgaggcgggc cggcggcaca cgtggcggcg gtacctcatc tggggcgagg
     4021 tgcacgacag taacgcatac gcgatggggg gagtcgccgg gcacgccggc gtgttcggca
     4081 ccgccgacga tctccttggt tacgcgcgga tgtggctggc cggcgggcgc acaccccgcg
     4141 gcgaggtgct gcccccggac ctggtccggg aggccacgac cgggcaggcg ccggcccgaa
     4201 cccgcgggct ggggtgggcg ctcagcgggc cccagggatg gtggggcgag tcgctctccc
     4261 cccgcgcgta cgggcacacc gggttcacgg ggacctcgat ggtgatcgat ccggagcacg
     4321 acctcgcgat cgtcctcctc acgaacgcga tccatctcgg gcgagaccgc ccggagatcc
     4381 tggccctccg gccccaactc gtggccgcgg tcgcgcacgc gctcctgtga gcgggcccag
     4441 ccccccccac gcccggggag gatcgtccga acggatccgc gaagccccct ccgccaatgg
     4501 cgtacttcct cggcgtcgac ggcggcgcga ccggcacgac ctgcgcggtg tgcgaccatg
     4561 acggccggat cctcggcatc ggtcgtggtg gtcccagcaa ccatatcctg gcgcccggcg
     4621 gggaggtgcg cgcccggtcc gcggtggaaa ccgcgctcgg cgccgccgaa gccgcatccg
     4681 ggctcgggcg cgtcgagttc gaatcggtcc agttcgggat gaccggcatc aaccgcgata
     4741 ccgagcaggc ccgggtgttc atgcgtgtga tcgcgaacgt cctcagcgcc cggatcgtgc
     4801 agatcgacaa cgatgcgtcg gtggcgctgg ccggcgcgct cgcctgccgg ccgggggtcg
     4861 tgttgatcgc agggacgggc tccgtggcgt ttggccggga tccctcgggc caggaagcgc
     4921 gggccggcgg gtgggggtac atcttcggag acgaagggag cgggtttgcg atcggcctg
//
LOCUS       DASQGH010000076         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48416, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000076 DASQGH010000000
VERSION     DASQGH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48416"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..833)
                     /gene="dapB"
                     /locus_tag="VEW91_01485"
     CDS             complement(33..833)
                     /gene="dapB"
                     /locus_tag="VEW91_01485"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019553115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HYM90286.1"
                     /translation="MHRVVVAGAAGRMGRAAVRTISRRDDMTLVGALGRKTALGQDAG
                     TTAGSGAIGVPITADLPELFLVGRPTVLVDLSRGEAAAEHALVALDHRVPAVIGATGL
                     PPEAVAVLRDRAEAQGVGALIAPNFALGALLMMEFARRAARFFPHVEIIELHHDRKRD
                     APSGTAARTARLVAEARGSAPAAAVTETTTVAGARGGSVDGIRIHSVRLPGLVAHQEV
                     LFGGPGQTLLIRHDSTSEESFMPGLVLAIQRVGALRHLVEGLEHVLDL"
     gene            complement(826..2133)
                     /locus_tag="VEW91_01490"
     CDS             complement(826..2133)
                     /locus_tag="VEW91_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYM90287.1"
                     /translation="MGTAMAVDQALYQRTVLPNGLVVITEPMDHVRTVAFGVWVAAGS
                     RFEDAGRNGISHFIEHILFKGTTTRSALAIAEAMDAIGGQLNAFTDKEHTCYYLRVLS
                     DHLDEGVAVLADMLLHPAIDPEALERERQVLAEEIKMVEDAPDDLVHDVFAETLWPGH
                     PLGRPISGSLASLAAISREDLRGYMAERYRPDAILVTAAGLLAHDRIVALVDDVLGAW
                     EGRATPCVHLPPRPHRATAYRSKEIEQVHLCLGVPGLPQAHPDRYVLAVLDSALGSGM
                     SSRLFQEIREERGLAYAISSYQAAYSDVGAFAVYAATSPQAVREVVRLSLDGLARARA
                     GLSPEEVARAKESLKGSLMLALETPGSRMTKLARSEQYFGRQITLDEILGDVDAVRGE
                     DVRRLAEELLVLDRLALATIGPFDQQPGLADGLEEEVRARAGA"
BASE COUNT          320 a          882 c          787 g          321 t
ORIGIN      
        1 atgatacccc gccatggatc ctcccctccc ggctacagat cgagcacgtg ctccagcccc
       61 tcgaccaggt gccgaagcgc gccgacccgc tgaatcgcga gcaccagccc gggcatgaac
      121 gattcctcgc tggtcgagtc gtgccggatc aggagcgtct ggcccggccc cccgaacagc
      181 acctcctggt gggccaccag tcccggcagc cgcacgctgt ggatccggat cccgtcgacc
      241 gacccacccc gcgcgcccgc gacggtcgtg gtctcggtca ccgccgcggc cggggccgat
      301 cctctcgcct cggccacgag gcgcgcggtc cgcgcggcgg tccccgacgg cgcgtcgcgc
      361 ttgcggtcgt ggtggagctc gatgatctcc acgtggggga agaatcgcgc cgcccggcgg
      421 gcgaactcca tcatcaagag cgcgccgagc gcgaagttcg gcgcgatcag ggcgccgacc
      481 ccctgggcct cggcgcggtc ccggagcacc gccaccgcct ccggggggag ccccgtcgcg
      541 ccgatcaccg cgggcacccg gtgatcgagg gccaccagcg catgctccgc cgcggcctcg
      601 ccccggctga ggtccaccag caccgtcggg cgccccacga ggaagagctc gggaaggtcc
      661 gcggtgatcg gcacgccgat cgcgcccgac ccggcggtcg tgcccgcgtc ctggccgagt
      721 gcggtcttgc ggcccagcgc ccccaccagc gtcatgtcgt cgcgccgcga gatcgtccgg
      781 acggccgcgc gccccatccg gccggcggca cccgccacca ccaccctatg cacctgcacg
      841 tgcgcgcacc tcctcctcga ggccgtcggc gagccccggc tgctggtcga acgggccgat
      901 cgtcgccagc gccagccggt cgagcacgag gagctcttcc gccaggcgcc gcacgtcctc
      961 gccccgcacc gcgtccacat cgccgagaat ctcgtccagg gtgatctgac ggccgaagta
     1021 ctgctcggag cgggccagct tggtcatgcg gcttcccggg gtctccaggg ccagcatgag
     1081 gctccccttg agggattcct tggcccgggc cacctcctcg gggctcaatc cggcacgcgc
     1141 ccgcgcaagc ccgtcgaggc tcaaccggac cacctcacga acggcctggg ggctcgtggc
     1201 cgcgtagacg gcgaacgcgc ccacgtcgct gtacgcggcc tggtacgagg agatggcgta
     1261 cgccagcccg cgttcctcgc ggatctcctg gaacaggcgg ctcgacatgc cgcttcccag
     1321 cgccgaatcg agcacggcga gcacataccg gtcggggtgg gcctggggca gccccggcac
     1381 gcccaggcag aggtggacct gctcgatttc cttgctgcgg tacgcggtgg cccggtgcgg
     1441 ccggggcggg aggtggacgc acggggtcgc ccgcccttcc cacgccccga ggacatcgtc
     1501 gaccagcgcg acgatccggt cgtgcgccaa gaggcccgct gcggtgacga ggatggcgtc
     1561 cgggcggtac cgctccgcca tgtagccgcg gagatcctcg cggctgatcg cggcgaggct
     1621 cgccaggctg ccggagatcg gccggcccag tggatggccg ggccagagcg tctccgcgaa
     1681 cacgtcgtgg accaggtcgt ccggggcgtc ctcgaccatc ttgatttcct cggcgaggac
     1741 ctgccgctcc cgctcgaggg cctcgggatc gatcgcggga tgcagcagca tgtccgccag
     1801 gaccgccacc ccctcgtcga ggtggtcgct caggacccgg aggtagtagc acgtatgctc
     1861 tttgtctgtg aacgcgttga gctgtccgcc gatcgcgtcc atcgcctccg cgatcgcgag
     1921 ggcagagcgg gtcgtggtcc ctttgaagag gatgtgctcg atgaagtgcg agatgccgtt
     1981 gcgaccggcg tcctcgaacc gggatccggc ggcgacccag acgccgaacg ccaccgttcg
     2041 cacgtgatcc atcggctcgg tgatgaccac caggccgttc ggcagcaccg tgcgctggta
     2101 gagggcctga tcaaccgcca tcgccgttcc cacccgaagc atcccccgcc gggccgcacc
     2161 cggcggccca caggtaaccg agaagcctcc tcgcggaggc ttctccaccg ccaggatcgg
     2221 ggcgcgtcac tacggcctcg gccctcgccg cgggggccgg cgcccccggt ctcggccgtg
     2281 atcttgttga tgaccttccc gccgggaccg
//
LOCUS       DASQGH010000077         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5999, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000077 DASQGH010000000
VERSION     DASQGH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5999"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VEW91_01495"
     CDS             <1..377
                     /locus_tag="VEW91_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90288.1"
                     /translation="QRVEDFESEYARGILENAPLKVLTRAADVDRVAPLLNLNDQHRA
                     LWKSLTQRRGEFSEALMIMDFLEGREGGVVVVRDIPEDYFIATTTAEERVRRDRLSAE
                     IGTWQAIQQLAESRRAACSRGG"
     gene            359..1306
                     /locus_tag="VEW91_01500"
     CDS             359..1306
                     /locus_tag="VEW91_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90289.1"
                     /translation="MFARGLRRMQGTALLLAAVVPVPLTPMVLPPTWPGAPTWWSDNG
                     NLSTSIADLRQFIETLQQSLERLTQWVQLYQQTAADILARTIWEFPGLLPDEADLTGL
                     IAQIGALPATLRQALELVRDKLQAPTVPGSVDERHHAYVESTPALVHEAVGITETDEV
                     VVGGMIQQAAASQAAAMGAAAVSRDLRPEAAGMEAQQASDALVSAAHDLPSSRAGIEL
                     LVAGMGAGLRQQAALGVAESDRLTVLVQQTAQVSQQIGALTATTGALTLRQADHDRRA
                     LDGTLGLADAVSTVAQLLQDVLTQAGDPAVSEPRLEPLY"
     gene            1409..>1697
                     /locus_tag="VEW91_01505"
     CDS             1409..>1697
                     /locus_tag="VEW91_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90290.1"
                     /translation="MMQLAWAVLTFLWLGHVAASMRPGCPPDVVGTFGRLVVSGGLLT
                     AVGSLNRIIVLAFEALRDAGSAVLSGLIGQNWHQFVQELLAPQAGVMFQTLG"
BASE COUNT          258 a          538 c          608 g          293 t
ORIGIN      
        1 gccagcgcgt cgaggacttc gagagcgagt atgctcgggg gatcctcgag aacgcgcccc
       61 tcaaggtgct gacccgcgcg gcggacgtcg accgcgttgc ccccctgctc aacctcaacg
      121 atcagcaccg agccctgtgg aagagcttga cgcaacgccg gggggagttc agcgaagcgc
      181 tgatgatcat ggatttcctc gaagggcggg agggaggggt cgtcgtggtc cgggacatcc
      241 ccgaagatta cttcatcgcc acaaccacgg cagaggagcg ggttcggcgg gatcggctct
      301 cggccgagat cggcacgtgg caggccatcc agcagttggc ggagtcgcgg agggcggcat
      361 gttcgcgcgg gggctgagga ggatgcaggg gactgcactt ctcctggcgg cggtggtccc
      421 cgtcccgctt acccccatgg tcctcccacc cacgtggcct ggcgccccca cctggtggtc
      481 cgacaatggg aacctctcga cgtcgatcgc ggatctcagg cagttcattg aaacactgca
      541 gcagtctctc gagcgcctga cgcagtgggt gcagctgtac cagcagaccg ccgctgacat
      601 actcgcgcgg accatctggg agtttcctgg cctcctccca gatgaggcgg atcttacggg
      661 tctgatcgcg cagatcgggg cgctgcctgc gacccttcgg caagcgcttg agttggttcg
      721 ggacaaactt caggccccga ccgtccccgg gagcgtcgac gagcgccacc atgcgtacgt
      781 cgagagcacc ccggcgctgg tgcatgaggc cgtgggcatt acggagacgg acgaagtggt
      841 tgtcggcggg atgatccagc aggccgcagc gtcccaggcg gccgcgatgg gagcggcagc
      901 ggtttcccgc gacctccggc cggaggcggc gggcatggag gctcagcagg ccagcgacgc
      961 actggtatcc gccgcgcacg atctgccgag ctctcgcgcg ggcattgagt tgttggttgc
     1021 aggcatggga gcgggcctgc gccagcaagc ggcgctgggg gtggcggagt ccgaccggtt
     1081 gaccgtgctc gtgcagcaga ccgcccaggt aagccagcag atcggcgcac tcaccgccac
     1141 gacgggagcg ctgacgctcc ggcaggcgga ccacgaccgc cgagccctcg atggaacatt
     1201 gggtttggcc gatgcggtct ccacggtggc tcaactgctg caagacgtct tgacgcaggc
     1261 gggcgacccg gcggtcagcg agccccggct ggagccgctt tactaggccg ggggttcgca
     1321 gccggccgtg ggcgggttcg agcgggggat cgctgaggtc tttgcttccc tggaaggctg
     1381 gcttgtctcc accggggcga cccggtcgat gatgcagctt gcgtgggcgg tgctgacgtt
     1441 tctgtggctc ggccatgtcg cggcctcgat gcgccccgga tgtccacccg atgtcgtcgg
     1501 gactttcggt cggctcgttg tctcgggggg gctcctgacc gccgtaggat ccctcaaccg
     1561 cattatcgtg ctggcctttg aggcgctgcg cgacgccggg tccgcggtcc tgtccggcct
     1621 catcgggcag aactggcatc agttcgtcca ggaattgttg gccccgcagg ccggtgtgat
     1681 gtttcaaacg ctcggtc
//
LOCUS       DASQGH010000078         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8840, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000078 DASQGH010000000
VERSION     DASQGH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8840"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VEW91_01510"
     CDS             complement(<1..372)
                     /locus_tag="VEW91_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM90291.1"
                     /translation="MRIEYTEEQRLIRETTREFAARELRPDARRWDAEGAFPPALVPK
                     LAELGLLGMIIPQEYGGSGLDMASMALAIEAVAWGDGGVALTVASHNSLCAGHIVRAG
                     TEAQKRAYLPRLASGASLGAWG"
     gene            complement(488..754)
                     /locus_tag="VEW91_01515"
     CDS             complement(488..754)
                     /locus_tag="VEW91_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HYM90292.1"
                     /translation="MDGQTGQVVTETSDLRARVEKVLDQIRPAVQQDGGDIELIDVAD
                     GIVQIRLAGSCVGCMYSMMTLQAGIERMLKEQIPEIKAVEAAPF"
     gene            complement(781..1173)
                     /locus_tag="VEW91_01520"
     CDS             complement(781..1173)
                     /locus_tag="VEW91_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYM90293.1"
                     /translation="MSVEVGRPTVILTDSAVAKLREMLAEQGDPTLCFRVFIQPGGCD
                     GFSYGMAFDAPEADDEVIERGGVRMLVDKTSSRLLRGAEIDYVTSVTATGFAIRNPNA
                     VSTCGCGHSFKTAEDTGQAEPCGKEDEG"
     gene            complement(1274..2446)
                     /gene="moeB"
                     /locus_tag="VEW91_01525"
     CDS             complement(1274..2446)
                     /gene="moeB"
                     /locus_tag="VEW91_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HYM90294.1"
                     /translation="MATKVTAARPQAPLLTAAQMERYSRQTILEELGVEGQAKLLRSK
                     VLIVGAGGLGSPSALYLAAAGVGTLGIVDGDQVDLNNLHRQILHTTTALGRPKTESAR
                     QTIAGLNPDVTVVPYQTVLTSANAFEMLGPYDVVINGSDNFPTRYLVNDACVLLGKPL
                     VDASILKWEGQATTFVPGRGCYRCLFPTPPPPGAVPSCAEGGIVGALCGYMGSRQALE
                     ALKLLLGVGETLANRLLIFDALEGETRIVRWNRNPECPVCGDRPTIRALIDYEQFCGM
                     PAHDRSAAPAKAPVPQVSAEEAKALLARGGVQLVDVREPWEHEEAHIPGCRLIPLGDV
                     AMRFPEIDRRTPVVVYCRSGSRSAKAVAFLLEQGYDRALNLAGGILAWANAQLPTE"
BASE COUNT          449 a         1000 c          871 g          437 t
ORIGIN      
        1 gccccacgcc ccgagggagg ccccggaggc gaggcgcggc aggtaggcgc gtttctgcgc
       61 ctcggtcccc gcacggacga tatgtccggc gcagagcgag ttatgggacg ccacggtgag
      121 ggccacccct ccgtcgcccc acgccacggc ctcgatcgcc agcgccatgc tggccatgtc
      181 gaggccggat ccgccgtatt cctgcgggat gatcatcccc agcagtccca gctccgccag
      241 cttggggacc aacgccgggg gaaacgcacc ctcggcatcc caccgccggg catcggggcg
      301 aagctcccgc gcggcgaact cgcgggtcgt ctcgcggatc aggcgctgct cctcggtata
      361 ctcgatgcgc acggtaccgg ctccggtcac gcgagggtgc acgcgcttgc gcgcgaaagg
      421 aaaaacctct catgaacttc acatgagagg ttcagggttc ctctcttgcc ccctccgtcg
      481 gaaggggtca aaacggcgcg gcttcgaccg ccttgatctc cgggatctgc tccttcaaca
      541 tccgctcgat gccggcctgg agcgtcatca tcgagtacat gcatcccacg cacgatcccg
      601 ccagccggat ctggacgatg ccgtccgcga cgtcaatcaa ttcgatgtcg ccgccgtcct
      661 gctggacggc cggccggatc tgatcgagga ccttctcaac ccgggcgcga aggtcagagg
      721 tctcggtgac cacctgcccg gtctgcccat ccatacgccg tcgctcctct cctggaaccg
      781 ctacccctcg tcctccttgc cgcagggctc ggcctgcccg gtgtcttccg cggtcttgaa
      841 ggagtgcccg cacccgcagg tggacaccgc gttggggttg cggatcgcaa acccggtagc
      901 cgtgaccgag gtcacgtaat cgatctccgc cccgcgcagc aggcgtgagc tcgttttgtc
      961 gacgagcatg cgcaccccgc cccgctcgat cacctcatcg tccgcctccg gggcatcaaa
     1021 cgccatgccg tacgaaaacc catcgcaccc gcccggctgg atgaacaccc ggaagcacag
     1081 ggtgggatcg ccctgctccg ccagcatctc ccggagcttg gcgacggcgc tgtcggtcag
     1141 gatgaccgta ggacggccca cttcgacgga catcgtgcgt cacctcacgt tgagccggtg
     1201 ctctctagcg tatccagact attgtagccg ccgccgggag cgcttgtcaa cgcgcggccc
     1261 gcggcttgcc gcctcactcg gtggggagtt gggcgttcgc ccaggccaag atgccgcccg
     1321 ccaggttgag cgcccggtcg tacccctgct cgaggagaaa cgccacggcc ttggcgctcc
     1381 gacttccgga cctgcagtag acgacgacgg gggtccgccg gtcgatctcc gggaagcgca
     1441 tcgccacatc gccgagcgga atcaggcggc aaccgggaat gtgcgcctcc tcgtgctccc
     1501 acggctctcg aacatccacc agctgcacgc cgccgcgggc gaggagggct ttggcctcct
     1561 ccgcggacac ctgcgggacc ggcgccttcg cgggcgccgc cgaacggtcg tgggcgggca
     1621 tcccgcagaa ctgctcgtag tcaatgagcg cgcggatcgt cggacggtcg ccgcacaccg
     1681 ggcactccgg gttccggttc caccggacga tccgcgtctc tccctcgagc gcatcgaaga
     1741 tcagcagccg gttggccagc gtctcgccaa cgcccagcag cagcttcagc gcctcgagcg
     1801 cctgtcggct ccccatatac ccgcacagcg cccccacgat ccccccttcc gcgcaactgg
     1861 gcacggcccc cgggggcggt ggggtgggga agaggcagcg gtagcacccg cggcccggca
     1921 cgaacgtcgt cgcctggccc tcccacttca gaatgcttgc atcgacgagc ggcttgccca
     1981 ggaggacgca ggcatcgttc accaggtacc gggtcgggaa gttgtcgctg ccgttgatca
     2041 cgacatcgta cggaccgagc atctcgaacg cattcgcgct ggtcagcacc gtctgatacg
     2101 gcacgacggt cacatccggg ttgagccccg cgatcgtctg ccgcgccgat tcggtcttcg
     2161 gccgacccag ggccgtcgtc gtgtggagaa tctggcggtg taggttgttc aggtccacct
     2221 gatcgccgtc cacgatcccc agcgtcccga cccccgcagc cgccagatac agcgctgacg
     2281 gggaccccag gcccccggct cccacaatca acaccttgct ccgcaggagc ttcgcctggc
     2341 cctcgacgcc cagctcctcc agaatggtct ggcgagagta ccgctccatc tgggcggcgg
     2401 tgagcaaggg agcctgcggc cgcgcagcag tcacctttgt cgccatcacg taccctccgt
     2461 tgctccgggc cgaaggcccg acattacacc ccacgataag acgcacccaa aagcctgtca
     2521 aggtgagaaa cgcgccgcgc agcatggtgc ttcccgaccc catccgggtc gcgcgcgagg
     2581 gggtaaatag cggaggggcg agtgctataa tgacccctgg ccccgccccg acactctgcg
     2641 cgataggcgt ggccaccgct gaccggcgcg cggggctgcg gggcatagta gaaccgggga
     2701 gagtacgggt ggagggttcg ggaatggcgc aggacctgtt cgacgtgaca atcctcg
//
LOCUS       DASQGH010000079         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51264, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000079 DASQGH010000000
VERSION     DASQGH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51264"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..510)
                     /locus_tag="VEW91_01530"
     CDS             complement(43..510)
                     /locus_tag="VEW91_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYM90295.1"
                     /translation="MRARERRIEPVHLPENAPRYIMPYTPALKVQGGTLVFAAGVTAA
                     PVYHSHPHVPAEFAAIPPDPAAQTQMAVANLRRVLEAAGGDLGDIVWLFRFIKDLDRN
                     QDAVNAVLGRALGTHRPASTTVEVVRLATDPRLVVELAVVAVVPGGARGPRRR"
     gene            complement(533..1300)
                     /locus_tag="VEW91_01535"
     CDS             complement(533..1300)
                     /locus_tag="VEW91_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90296.1"
                     /translation="MGASLLKPVPLLAVEHLTVAYDDVPAVAGISFEVHAGEAVAIIG
                     SNGAGKTTTLNALVGLVRSRTGTVTFDGGRIDAAAAHDIHARGLILIPEEQQTFPAMT
                     VWDNLMMGGYARRNRPGLAARVDESLALFPRLRERRRQPAGSLSGGEQRMLAIARALV
                     GRPRLLLLDEPSTGLAPRIVDDLFDVLGHLRTRGLTCLLVEQNAVQALQFADRAYVLE
                     TGRIVHQGPAKTLLSDPQVASFYLGTGGPGGAQHAGT"
     gene            complement(1282..2043)
                     /locus_tag="VEW91_01540"
     CDS             complement(1282..2043)
                     /locus_tag="VEW91_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90297.1"
                     /translation="MAETPRAAVGATGLTKRFGGVVALDGVDLVLFPGQIVGLIGPNG
                     SGKTTLFNVLTGFVRPDAGTVMVGDRDMTCRAPEDVCRAGIARTFQTSRPFRHMTVED
                     NVLVALLHAGRRPLRRVRAEAAGLLDQFGLTPLAASPAAGLTLTQRKRLEMARALATG
                     PAVLLLDEVAAGLSAREVDAMGALIDRLRRERELAVCVVEHVMRMVMQISERIVVLDY
                     GQKIAEGPPRAIISDVRVVEAYLGRYAQAAWGRPS"
     gene            complement(2036..>2789)
                     /locus_tag="VEW91_01545"
     CDS             complement(2036..>2789)
                     /locus_tag="VEW91_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90298.1"
                     /translation="LGQSLGGVPVWLLPCGALVAGAFALLIGYPTLRIRGPHFAVATL
                     ILALMGEIFVGNLDRITGGGLGIVLPRPQGTPFLVERRFYFVFGAMAAIAALAAWWVE
                     HSALGYTLQAIREDEDAARAVGIRAARYKILMLCLSACGAGAVGGLYGSYVGYISPDG
                     AFDLAFSVKPVVYAVIGGLGTWAGPVIGSGLMEIVSTLLTLLSPTLKAVSQVLFGAIL
                     VAVVLAAPQGITGWLARRRAPRPRARWEAARG"
BASE COUNT          420 a         1025 c          992 g          352 t
ORIGIN      
        1 gagccagacc gtccccgcga gggggcggat gctcggggtg ggttagcggc ggcgcgggcc
       61 gcgtgcgccg cccggcacca ctgcgaccac cgccagttcc acgacgagcc gcggatcggt
      121 cgcgagccgg acgacttcga cggtcgtgct ggccggccgg tgcgttccca gggcccgccc
      181 cagcacggcg ttgaccgcgt cctgattgcg gtcgaggtcc ttgataaacc ggaagagcca
      241 cacgatgtcc cccaggtcgc cgccggccgc ctcgagcacg cgccggagat tcgccaccgc
      301 catctgcgtc tgcgccgcgg gatccggcgg gatcgcggcg aactccgcgg gcacgtgcgg
      361 atggctgtga tacacggggg cggccgtcac cccggcggca aagaccagcg tgcccccctg
      421 caccttgagg gccggggtgt atggcatgat gtacctgggg gcgttttccg gcaggtgcac
      481 gggctcgatg cggcgctccc gcgcccgcat gatacggccc cgccctcccg ccttacgtgc
      541 cggcatgctg cgcgcccccc ggtccgcccg ttccgaggta gaagctcgcc acctgaggat
      601 cactgagcag cgtctttgcg gggccctggt ggacgatccg gccggtttcg agcacatacg
      661 cccggtcggc gaactggagc gcctgcacgg cgttctgttc cacgaggagg cacgtcagcc
      721 cccgtgtccg gaggtggccg aggacatcga agagatcgtc gacgatgcgt ggggcgagcc
      781 cggtggaagg ctcgtcgagg agcaggagcc gcggccggcc gacgagcgcc cgggcgatcg
      841 cgagcatgcg ctgctcacct ccgctgaggc tcccggcggg ctggcgccgc cgctcgcgga
      901 ggcggggaaa gagcgccagg ctctcgtcca cgcgggcggc gagaccaggc cggttccggc
      961 gggcataacc gcccatcatc aaattatccc acacggtcat cgccgggaac gtctgctgct
     1021 cttccgggat caggatcagc ccccgtgcgt ggatgtcgtg ggccgccgcg gcgtcgatgc
     1081 gcccgccgtc gaacgtcacc gtgcccgtcc gggagcggac cagccccacc agcgcgttca
     1141 gcgtcgtggt cttccccgcc ccgttcgagc cgatgatcgc cacggcctcc cctgcgtgga
     1201 cctcgaagga gatcccggcg accgccggga cgtcgtcgta ggcgacggtc agatgctcga
     1261 cggcgaggag gggcaccggc ttcaggaggg acgcccccac gcggcctggg cataccggcc
     1321 gaggtacgct tcgacgacgc ggacgtcgga gatgatcgcc cgcggcggcc cttccgcgat
     1381 cttctgcccg tagtcgagta ccacgatgcg ctcggagatc tgcatgacca tgcgcatgac
     1441 gtgctccacg acgcagacgg cgagctcccg ctcgcgccgc aggcggtcga tcagcgcgcc
     1501 catcgcatcg acctcgcgcg cgctcagtcc cgccgccacc tcgtcgagca gcagcaccgc
     1561 cggccccgtg gcgagcgcgc gggccatctc gaggcgcttc cgctgggtca gggtcaggcc
     1621 cgccgcgggc gaggccgcga gcggcgtgag gccgaactgg tcgagcagcc cggcggcctc
     1681 cgcgcggacg cgccggagcg gcctgcggcc ggcgtggagg agcgcgacga ggacgttgtc
     1741 ctcgacggtc atgtggcgga acgggcggct cgtttgaaag gtccgcgcga tgccggcgcg
     1801 gcacacgtcc tccggcgcgc ggcacgtcat gtcgcggtcc cccaccatca ccgtgccggc
     1861 atccggccgc acgaatcccg tgagcacgtt gaacagcgtc gtcttccccg agccgttcgg
     1921 cccgatcaac cccacgatct ggcctgggaa gagcacgagg tcgaccccgt ccagggcgac
     1981 gacaccgcca aaccgcttgg tcagtccggt ggccccgacc gccgcccggg gggtctcagc
     2041 cacgggccgc ttcccatcgc gcccgcgggc gcggggcccg ccggcgcgca agccagccgg
     2101 tgatgccctg cggcgcggcg aggacgaccg ccaccaggat cgcgccgaac aggacctggc
     2161 tgacggcctt gagggtgggt gacaggagcg tgagcagcgt ggacacgatc tccatgagcc
     2221 cggagccgat caccggcccg gcccaggtgc cgaggcctcc gatgacggcg tagacgaccg
     2281 gtttgacgga gaaggccaga tcgaaggccc catcgggcga gatgtagccc acgtaggagc
     2341 cgtacaggcc gccgaccgcc cccgcgccgc acgcgctcag gcagagcatg aggatcttgt
     2401 accgggccgc ccggatgccc accgcgcgcg cggcgtcctc gtcctcgcga atggcctgca
     2461 gggtgtaccc gagggcactg tgctccaccc accaggcggc cagggcggcg atcgcggcca
     2521 tcgccccaaa gacgaagtag aaccgccgct cgaccagaaa cggcgtcccc tgcgggcggg
     2581 ggagcacgat ccccagccct ccgccggtga tgcggtcgag attcccgacg aagatctccc
     2641 ccatcagggc caggatcagc gtggcgaccg caaagtgggg gccccggatc cgcagcgtcg
     2701 gatacccgat caggagcgca aacgcgccgg ccacgagcgc cccgcacggc agcagccata
     2761 ccggcacccc ccccagggac tgcccgagg
//
LOCUS       DASQGH010000080         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8846, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000080 DASQGH010000000
VERSION     DASQGH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8846"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VEW91_01550"
     CDS             <1..184
                     /locus_tag="VEW91_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYM90299.1"
                     /translation="VTKHITLLEEAGIVVTLRKGREKLHYLNPVPIHEIAERWIRPFE
                     RSKLEALRDLKARLEG"
     gene            188..658
                     /locus_tag="VEW91_01555"
     CDS             188..658
                     /locus_tag="VEW91_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYM90300.1"
                     /translation="MAETFVYATYIRTTPEKLWEALTNGDFSEKYWMGFRIEVELKAG
                     GSVRILPPKDPERYGDHAGKVLVCEPLRKLVYTFAPKDKPEVAAKRDGPSRVTYELTP
                     MGPMVKLRLIHENLLPEDLEKDPNKFQGVNNGWPAVMSSLKSLLETGKAITFEF"
     gene            679..1203
                     /locus_tag="VEW91_01560"
     CDS             679..1203
                     /locus_tag="VEW91_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYM90301.1"
                     /translation="MAITNETQARGPRAVRVEATVKASAAEVWRAWTTSEGAEEFFAQ
                     KAIIRLAIGGPYEINFNPEDARQGTKGLKILSYAPEEMLSMQWNAPPEFSEVRNGGTW
                     VVVQMRPEGADRTRVTVTHLGWKEGTEWDRAFPHFTRGWGDLMRRLERRFAEGPIDWS
                     KESMMWKEQAKSQR"
     gene            1293..1529
                     /locus_tag="VEW91_01565"
     CDS             1293..1529
                     /locus_tag="VEW91_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90302.1"
                     /translation="MRTCSQCGKAFTPGAAGWMARLEGPPGGVHPGAALRTVLLCPED
                     FARLGPAQKAMWHEHIDPPRDGPTREKRPGRLSA"
     gene            complement(1550..2689)
                     /locus_tag="VEW91_01570"
     CDS             complement(1550..2689)
                     /locus_tag="VEW91_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HYM90303.1"
                     /translation="MNVYLDHAATTPVDPRVLQAMLPFFTERAGNASSVHGFGQDARG
                     AVDQARAQVAGAIGARPAEIVFTSGATESDNLAVMGVVLAAEGRGRHLVTTAIEHHAV
                     LEVCHFLEARGCSVTYVPVDGRGIVDPDDVRRALRPDTVLVSVMAANNEIGTLQPVAE
                     IGLLTRERSIPFHTDATQLVGAAPVDVDDLHVDLLSMSAHKRYGPKGVGALYVRTGTP
                     LAPVQRGGSHERGRRGGTENVPGIVGLGAALRIALEEMEGERVQLTALRDRLVREALG
                     LPGAHLNGDPVQRLPNNVNLSFDDTDSQSLVMGLDLHGVASSSGSACTSGSMEPSHVL
                     VALGLPPARATAAVRLTLGRGTTDAEITCALGALRDVVARLRRAA"
     gene            complement(2686..3057)
                     /locus_tag="VEW91_01575"
     CDS             complement(2686..3057)
                     /locus_tag="VEW91_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYM90304.1"
                     /translation="MQDLEFPFLTPRIVLGLTGISAETLRRWEAAGVPASGRRRAHQG
                     RAAGPRLYSWREVEQLQQATYLRRTTRLTLGEVKRLLKRRQAAARGRDWVIARPKPRA
                     RGARLFGGAGAVPGAPTRRMR"
     gene            complement(3057..3824)
                     /locus_tag="VEW91_01580"
     CDS             complement(3057..3824)
                     /locus_tag="VEW91_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYM90305.1"
                     /translation="MSSRSHRLNQERFSRTAELFAANRAVERLSQVDSLFHLAAPTRE
                     DLLLDVACGPGRLLATCAPRIRMAVGLDLTLEMLRIARRASGQGAHVLLVLGEGERLP
                     FPDGTFTLVTTTLAIHHYAEPRRVVEEMVRVCRAGGRVAVGDTVGESDEAKRARQNEI
                     ERLRDPSHVELLSPKGLEALLTSCGLEVAGRASGSIVREFGEWCRVAGTPPQVAARVR
                     AALLETRAGDLAGMAPVLAGEELRFRHNWMNIVCRRP"
BASE COUNT          649 a         1372 c         1399 g          538 t
ORIGIN      
        1 cgtgacgaag cacatcaccc ttctcgaaga agctggaatc gtggtcacgc ttcgcaaggg
       61 gcgcgaaaag ctgcattacc tgaaccccgt tcccattcac gagatcgccg agcgctggat
      121 ccgcccgttc gaacgcagca agcttgaggc gctgcgagac ttgaaagctc gactagaggg
      181 gtgagttatg gcagaaacgt tcgtttatgc gacctatatc cgcacgacgc ctgaaaagct
      241 ctgggaagcg ctaacgaacg gcgacttcag cgagaaatac tggatgggct ttcgcatcga
      301 ggtcgaactg aaggccggcg gcagcgtccg catcctcccg cccaaggacc cggagcgcta
      361 cggagaccac gccggcaagg tcctggtctg cgagccgctg cgcaagctcg tctacacctt
      421 cgcccccaag gacaagcccg aggtcgccgc caagcgcgac ggcccctcgc gcgtcaccta
      481 cgagctcacg ccgatggggc cgatggtgaa gctccggctg atccatgaga acctgctgcc
      541 cgaggacctc gagaaggatc cgaacaagtt ccagggcgtc aacaacggct ggccggccgt
      601 catgagcagc ctcaagagcc tcttggagac agggaaagcg atcaccttcg agttttaggg
      661 atcaggaggg ggaaaaagat ggctatcacc aacgaaacgc aagctcgagg tccgcgggcg
      721 gtccgcgtcg aggcgacggt gaaggcctct gcggccgagg tctggcgcgc ctggaccacg
      781 tcggagggtg ctgaggagtt cttcgcgcaa aaggcgatca tccggctggc gatcggcgga
      841 ccctacgaaa tcaatttcaa cccggaggac gcgcggcagg ggacgaaggg tctcaagatc
      901 ctgagctacg cccccgagga gatgctctcg atgcagtgga acgcgccgcc ggaattctcg
      961 gaggtgcgca atggcgggac ctgggtcgtg gtgcagatgc ggcccgaggg cgccgaccgg
     1021 acgcgcgtca cagtgacgca cctgggctgg aaggaaggca ccgagtggga ccgggcgttt
     1081 ccgcacttca cgcgcgggtg gggcgaccta atgcggcgct tagagcgccg cttcgccgag
     1141 gggcccatcg actggagcaa ggagtcgatg atgtggaagg aacaagcgaa gtcgcagcgg
     1201 tagtgccgcg agtctcggcc gaaatccccg cggcggtcga ccagaaggtc aatcatgaag
     1261 ggtgtcccca tcctccataa ggagggactc ggatgcggac gtgctcacag tgcggcaagg
     1321 cgttcacgcc gggggcggcg ggatggatgg cgcggctcga gggcccgccc ggcggggtcc
     1381 atccgggggc ggcgctccgg acggtgttgc tctgcccgga agatttcgcc aggctcgggc
     1441 cggcgcagaa agcgatgtgg cacgagcaca tcgatccccc gagggacggc ccgacgcgcg
     1501 agaagcggcc cgggcgcctg agcgcctgag cgcctgaggg gccccggcgt caggccgcgc
     1561 gcctgagtct cgccactacg tcgcggagcg ccccgagcgc acaggtgatc tccgcgtcgg
     1621 tcgtcccgcg gccgagggtg agccggacgg ccgcggtggc gcgcgccggc gggagaccca
     1681 gggccacgag cacgtgggag ggctccatgc tgcccgaggt gcacgccgac ccggagctgg
     1741 acgcgacccc gtggagatcg agccccatga cgagcgactg gctgtcggtg tcgtcgaagg
     1801 acaggttgac attgttgggc agccgctgca cggggtcgcc gttgaggtgg gccccgggca
     1861 acccgagggc ctcgcgcacg aggcggtccc ggagggcggt gagctggacg cgctcgccct
     1921 ccatttcctc cagggcgatc ctgagcgcgg cccccaagcc cacgatcccc ggcacgttct
     1981 ccgtgccgcc gcgacgtcca cgctcgtggc tgccgccccg ctggaccgga gccagggggg
     2041 tgcccgtccg cacgtacagc gcgcccaccc ccttcgggcc gtagcgcttg tgcgcggaca
     2101 tcgagagcag gtcgacgtgg aggtcgtcca cgtcgaccgg cgcggccccg accagttggg
     2161 tcgcgtcggt gtgaaacggg atgctgcgct cgcgcgtgag gagtccgatt tccgccacgg
     2221 gctggagcgt gccgatctcg ttgttggcgg ccatcaccga cacgagcacg gtgtccggcc
     2281 tgagcgcccg ccgcacgtcg tcgggatcca cgatcccccg cccgtcgacc gggacgtagg
     2341 tgacggaaca cccccgcgct tcgaggaaat ggcagacttc gaggaccgcg tggtgctcga
     2401 tcgccgtggt cacgagatgc cgcccgcggc cctcggccgc cagcaccacg cccatcacgg
     2461 ccagattgtc cgactccgtc gccccgctcg tgaacacgat ctccgcgggt cgcgcgccga
     2521 tggcgccggc cacctgcgcc cgcgcctggt cgacggcgcc ccgggcgtcc tgcccgaacc
     2581 cgtgcacgct gctcgcgttg cccgcccgct cggtaaagaa cggcagcatc gcctggagca
     2641 cccggggatc aacgggggtg gtcgccgcgt ggtccaggta gacgttcacc gcatccgcct
     2701 ggttggagcg ccgggaacgg cccccgcgcc cccgaacagg cgcgcgccgc gggcgcgggg
     2761 cttgggccgg gcgatcaccc aatcgcgacc gcgcgcggcg gcctggcgcc gcttgaggag
     2821 gcgcttgacc tcgccgaggg tgaggcgcgt ggtcctgcgg agataggtgg cctgctgaag
     2881 ttgctcgacc tcccgccacg agtagagcct ggggccggcg gcacgcccct ggtgggcccg
     2941 gcggcggccg gaggccggca cccccgccgc ctcccagcgc cggagcgtct ccgccgagat
     3001 cccggtgagg cccagcacaa tgcggggggt cagaaatggg aactccagat cctgcatcag
     3061 ggcctccggc agacgatgtt catccagttg tgtcgaaacc tgagctcctc acccgcgagc
     3121 acgggggcca tccccgcgag gtcgcccgcc cgggtctcga ggagcgcggc gcggacccga
     3181 gcggccacct ggggaggggt cccggcaacc cggcaccatt ccccgaactc gcggacaatc
     3241 gacccggagg cccgccccgc gacctcgagg ccgcacgagg tgaggagcgc ctcgagcccc
     3301 ttcgggctca gcagttcgac gtgggaggga tcccgaagcc gctcgatctc gttctgccgg
     3361 gcccgcttcg cctcgtcgga ttcccccacc gtgtccccga ccgccacccg tcccccggcc
     3421 cggcagaccc gcaccatctc ctcgacgacc cggcggggct ccgcgtagtg gtggatcgcc
     3481 agcgtcgtcg tcacgagcgt aaacgtcccg tccggaaacg ggaggcgctc cccctcaccg
     3541 agcacgagga ggacgtgggc tccctgccca gaggctcggc gggcgatcct gagcatctcc
     3601 agcgtgaggt cgagcccgac ggccatccgg atccgcgggg cacaggtcgc gagcagacgg
     3661 cccgggccgc aggcgacgtc cagcaggagg tcctcccgcg tcggggccgc caggtggaac
     3721 agggagtcca cctgcgacag ccgctcgacc gcccggttcg cggcgaagag ctcggcggtc
     3781 cggctgaacc gttcctggtt taggcgatgg gacctggaac tcacctgggc ctcacgcgag
     3841 cggcaccatc ctgcgggtcc gggatgcgaa cgtcgccacc tcagtatacc ccaccgcgcg
     3901 cgcgtaggcc gccgcccggc cgaagtctcg gcccacgtcc tccgggatgt gcgcgtcc
//
LOCUS       DASQGH010000081         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14542, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000081 DASQGH010000000
VERSION     DASQGH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14542"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VEW91_01585"
     CDS             <1..453
                     /locus_tag="VEW91_01585"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HYM90306.1"
                     /translation="SHLPRSHALMAGLAESLARRNRRGLKVIASSMAGLGPGLTPACD
                     DLLAGVLLALALTQEQQADAELGEIAVMLVDTAASRTHEISAAYLRAAQAGEAHERWH
                     RLLAALAGDNPGAIDEAAHAVMAVGETSGADMLAGFLIAMRIMHGAPV"
     gene            complement(458..2218)
                     /locus_tag="VEW91_01590"
     CDS             complement(458..2218)
                     /locus_tag="VEW91_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha/beta"
                     /protein_id="HYM90307.1"
                     /translation="MFRRPPAVETVACPSCDLANPGRALEERLYICAGCGHYLPMPSP
                     ARIAMLADPGTFREVDRNLISVDPLQFIDRKPYRERLVEARRQTGLREAATSGLCQIA
                     GRRVVLIVFDFEFLGGTMGSVVGEKVANAFERAVRRRIPVVSVAASGGARMQEGMLSL
                     MQMAKVSAAAARHDREGLAFISILTDPTFGGVTASFASLGDVMIAEPGTQIGFVGPRV
                     IEQTTGTSLPPDSHRAETLLRAGMLDLVVPRERLRETVAYLVGQLSREKGARGRSRAG
                     APPPAISALPAWEQVRLARLAARPQAQDYIAAMTARFVELHGDRQGGDDPAVIGGLAE
                     LDGDTVVIVGHQRWRTPEAATAPFQGMAYPEGYRKALRLMQLGAKFQLPVVTLIDTRG
                     ANQSYESEQRGIAQALARNLAAMSCLPTPIVAVIIGEGGSGGALALGVADRVLMLEHA
                     IYSVISPEGAAAILYRDAQKAEALSEALKLTAQDLHRLGVIDTIVPEPEGGAHLDPPR
                     AAALVKQHVVAALRELRRVPVPRLLAHRYAKYRHVGRVGVYWREIARQEMQEALDALS
                     RRLLRSEGVRRRIRGERPGV"
     gene            complement(2220..3410)
                     /locus_tag="VEW91_01595"
     CDS             complement(2220..3410)
                     /locus_tag="VEW91_01595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYM90308.1"
                     /translation="MPGVGPLSDLLVVDLTRALAGPYCTMMLADFGARVIKVETPEGG
                     DDTRGWGPPFCGGESAYFLSINRNKQSLTLNLKDPRGHDLLLRLLKRADVVVENFRPG
                     TMDRLGLGYAAVHAMFPRLVYCSISGFGQTGPYRERAALDLIVQGMGGIMGITGEPDG
                     PPTRVGVAITDICAGMFAAYGILAALRVRERTGTGQWVDAAMLDGQVAWMTYMAANYF
                     ATGEDPPRAGSAHLNLVPYQPFRTRDGIVNVAAATEGLWQRFCSALEAPVANDPRFAT
                     NADRVRNRGALLDMLRPVFVQRTTAEWVERLLRAGVPAGPIYRMSEVMEDPQVHHRGM
                     VVEMEHPRAGRIRVNGVPVKLSDTPGAVTTPPPVLGEHSEAILRDLGCDPGEIAVLRR
                     DRVI"
     gene            complement(3416..>3917)
                     /locus_tag="VEW91_01600"
     CDS             complement(3416..>3917)
                     /locus_tag="VEW91_01600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYM90309.1"
                     /translation="MWERKHKIRIQRGDILVIHTGYHHYYNEDWYKVRNKEKPNLPRA
                     FLRHPGPQLEFCDWVLGRGIRWIAIDAVSTDHPFNTNVRRARPDLIPEVEKKLGMPVD
                     RAFPWPKAYQATHTYLFPRGGFHVENVGGMIDEGLNMRVWFGAFPFRFKGGEAAFCRC
                     FAFVRK"
BASE COUNT          584 a         1454 c         1310 g          569 t
ORIGIN      
        1 agccatctgc cacgcagcca cgcgttgatg gccgggctcg ccgagtccct cgcgcgccgc
       61 aaccggcgcg ggctcaaggt catcgcctcg tcgatggccg gactcgggcc ggggctcacg
      121 ccggcgtgtg acgatctcct cgccggggtg ctgctcgcgc tggcgctgac ccaagagcag
      181 caggccgacg ccgaactcgg cgagatcgcc gtcatgctgg tcgacacggc ggcgtcccgc
      241 acgcacgaga tcagtgccgc gtacctgagg gccgcgcagg cgggcgaggc gcacgagcgg
      301 tggcaccgcc tcctcgccgc cctggccggc gacaaccccg gcgcgatcga cgaggcggcg
      361 catgccgtga tggcggtcgg ggaaacgtcg ggggccgata tgctcgccgg gttcctcatc
      421 gccatgcgga tcatgcacgg cgctcccgtc taaccgccta cacccccgga cgctctcccc
      481 ggatccttcg ccgcaccccc tcggaccgca agaggcgccg gctcagggcg tcgagcgcct
      541 cctgcatctc ctggcgggcg atctcccgcc agtagacgcc gacgcggccg acgtgccggt
      601 acttggcgta gcggtgggcg agcagccgcg ggaccggcac ccggcgaagc tcacgcagcg
      661 ccgctaccac gtgctgcttc accagggctg ccgccctcgg gggatcgagg tgcgccccgc
      721 cctcgggttc aggcacgatg gtatcgatga cccccagccg gtggagatcc tgcgccgtca
      781 gtttcagcgc ctcggacagc gcctccgcct tttgggcatc ccggtagagg atggccgccg
      841 cgccctcggg ggagatgacc gagtagatgg cgtgttcgag catcaggacc cggtcggcga
      901 cgccaagggc cagcgcgccg ccgctccccc cctcgccgat gatcaccgcc acgatcgggg
      961 tggggagaca ggacatcgcc gccaggttgc gcgccagggc ctgggcgatc ccgcgctgct
     1021 cggactcata gctctggttc gcgccgcggg tgtcgatgag ggtcacgacc gggagctgga
     1081 acttggcgcc caactgcatc aggcggagcg cttttcgata gccttccgga taggccatcc
     1141 cctggaacgg cgccgtcgcc gcctccggcg ttcgccaccg ctgatggccc acgatgacca
     1201 cggtatctcc gtccagctcc gccagcccgc cgatgaccgc ggggtcgtcc ccgccctggc
     1261 gatcgccgtg caattcgaca aaccgcgcgg tcatggccgc gatgtagtcc tgcgcctgtg
     1321 ggcgcgccgc gagccgcgcc agccggacct gctcccacgc cggcagcgcg ctgatcgccg
     1381 gggggggcgc gccggcccgg gaccggcccc gcgcgccctt ctcccggctc agctgcccca
     1441 cgaggtacgc cacggtctcc cggagacgct cccgggggac gacgaggtcg agcattcccg
     1501 cgcgcagcag ggtctccgcg cggtgggagt ccggcggcag cgacgtcccg gtcgtctgct
     1561 cgatgacccg cgggccgaca aatccgatct gggtcccggg ctcggcgatc atcacgtcgc
     1621 cgagcgacgc gaagctcgcg gtcaccccgc cgaaggtcgg gtcggtgagg atcgagatga
     1681 acgccaggcc ctcgcggtcg tgccgggcgg ccgccgccga gaccttggcc atctgcatga
     1741 gcgacagcat cccctcctgc atccgcgcgc cgccgctcgc ggcaacgctg accactggga
     1801 tgcgtcggcg gacggcgcgc tcgaaggcgt tggccacctt ctcccccacc accgagccca
     1861 tcgtgccgcc caaaaactcg aaatcgaaca cgatgagcac gacccggcgg cccgcgatct
     1921 ggcagaggcc gctcgtcgcc gcttcgcgca gtccggtctg ccggcgggcc tcgaccagcc
     1981 gctctcggta cggcttccgg tcgatgaact ggaggggatc gacggaaatg aggttccggt
     2041 ccacctcgcg aaaggtgccg ggatcggcca gcatcgcgat ccgggccggc gacggcatcg
     2101 ggaggtagtg cccgcagccc gcgcagatat acaaccgctc ctcgagcgcc ctccccgggt
     2161 ttgcaaggtc gcaactcggg cacgccaccg tctcgaccgc gggaggacgc cggaacacct
     2221 cagatcaccc tgtcgcggcg gagcacggcg atctccccgg gatcgcaccc gaggtcgcgc
     2281 aggatcgcct cggagtgctc ccccagcacc ggcggcgggg tggtcaccgc cccgggcgtg
     2341 tcggagagct tcaccggcac cccgttgacc cggatccgcc ccgcgcgggg atgctccatc
     2401 tccaccacca tcccccggtg gtggacctgg ggatcctcca tgacctcgct catccggtag
     2461 atcggacccg ccggcacccc ggcgcgcagc agccgctcca cccactcggc cgtggtccgc
     2521 tgcacgaaca cgggccggag catatcgagc aacgccccac ggttgcgcac ccggtcggcg
     2581 ttcgtggcga agcgcgggtc gttggccacc ggggcctcca gcgcactgca gaaccgctgc
     2641 cagagcccct cggtcgccgc cgcgacattc acgatcccgt cgcgggtccg aaacggctga
     2701 tacggcacga ggttgaggtg ggccgagccg gcgcgcggcg gatcctcgcc ggtcgcaaag
     2761 tagttcgcgg ccatgtacgt catccaggcg acctggccat cgagcatcgc cgcatcgacc
     2821 cactgccccg tcccggtccg ctcgcgcacg cggagcgcgg ccagaatgcc gtaggcggcg
     2881 aacatcccgg cgcagatgtc ggtgatcgca acccccaccc gcgtcggcgg cccgtcgggc
     2941 tcgccggtga tgcccatgat cccgcccatc ccctgcacga tcaggtccaa cgccgcccgt
     3001 tctcggtatg gtccggtctg cccgaacccg gagatcgagc agtacacgag acgggggaac
     3061 atcgcgtgga cggcggcgta ccccagcccc agccggtcca tcgtgccagg ccggaagttt
     3121 tcgaccacga cgtccgcgcg cttgagcagc cggaggagga ggtcgtgccc tcggggatcc
     3181 ttcaagttca gggtcaggct ctgcttgttt cggttgatgc tgaggaagta ggcgctctcc
     3241 ccgccgcaga acggcggtcc ccacccccgg gtatcgtccc ctccctccgg ggtctccacc
     3301 ttgatgaccc gcgcgccgaa gtcggcgagc atcatcgtgc agtacggacc cgccagcgcc
     3361 cgggtcaggt ccaccaccag cagatcgctg agggggccca cgcccggcat cgccttcact
     3421 tccggacgaa cgcgaagcac cggcagaacg ccgcctctcc gcccttgaag cggaacggga
     3481 aggcgccgaa ccagacccgc atgttcagcc cctcgtcgat catcccgcca acgttctcga
     3541 cgtggaaccc gcccctggga aacaggtagg tgtgggtcgc ctgatacgcc ttcggccagg
     3601 ggaaggccct gtcgaccggc atcccgagct tcttctcgac ctcggggatg agatccggcc
     3661 gggcccgccg gacattggta ttaaatggat ggtccgtgga gacggcatcg atcgcgatcc
     3721 accggatccc ccgccccagc acccagtcac agaactcgag ttgcgggccg gggtgcctga
     3781 ggaaggcgcg ggggaggttc ggcttctcct tgttgcgcac cttgtaccag tcctcgttgt
     3841 agtagtggtg gtagccggtg tggatgacga ggatgtcccc ccgttggatg cggatcttgt
     3901 gcttgcgctc ccacatc
//
LOCUS       DASQGH010000082         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_334, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000082 DASQGH010000000
VERSION     DASQGH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..294
                     /locus_tag="VEW91_01605"
     CDS             109..294
                     /locus_tag="VEW91_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90310.1"
                     /translation="MDPEGPVIIWTRALLRLPAATGERFAEEWKSVRRRVEVVHATAV
                     SHDVEPAGAPRLDAPDE"
     gene            287..1654
                     /locus_tag="VEW91_01610"
     CDS             287..1654
                     /locus_tag="VEW91_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYM90311.1"
                     /translation="MSEPWELSLSEAASAIAAGRLSPVDLVESTLDRIERVEPRVQAW
                     ARLRTEDARREAGRLAQLRRAGTILGPLHGIPIGVKDIFNTAGVETACGSRIMAGFVP
                     AADATAVARLRASGAIILGKTHTTEFASFDPSPARNPWALDHTPGGSSSGSGAALSAR
                     MCQGALGSQTSGSIIRPSAFCGIVGLKPTFGRVSRHGVHPLAWTLDHPGPMARTVRDV
                     AVLLEAVAGPDAHDPATLSAPPPPQYAALLDEQPDAVAGLRVGIPDRYFTDGVDPEAE
                     ELYRTALRVLETVGCAVREIRLPPEFEAGMEAHELIHSAEAAAVHLDRYRARPDDYSS
                     KLRAIIETGLQIPAPTYVRAQQIRTVVIRGLRAVVRDVDVIATPAALGPAPEGLASTG
                     SPVLNRPFSFTGFPSLTVPCGFTEAGLPVGVQLAGRPFDEATLLRIAAAYEHATGWGR
                     RVPPL"
     gene            complement(1724..>1942)
                     /locus_tag="VEW91_01615"
     CDS             complement(1724..>1942)
                     /locus_tag="VEW91_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90312.1"
                     /translation="RGPWGQPGGSAGPRLDRVGEIGRKVALSAAVVAAALMLVRVLRS
                     PTLDLADLLAMLFVLLRIWGQASPSGRA"
BASE COUNT          273 a          713 c          700 g          256 t
ORIGIN      
        1 tctcccccgc cccgcaggag gtctccgagc gaaccgccaa cagcgtttct acgcccgtca
       61 caagcgccgg gcgcgtcgtc tcgagacgcc tgcgccgggg aggatggcat ggaccccgaa
      121 ggaccggtca ttatctggac ccgcgcactg ctgagactgc cggccgccac gggcgagcgc
      181 ttcgcggagg agtggaagag cgtcaggcgc cgggtggagg tcgtgcacgc gactgcggtg
      241 tcgcacgacg tcgagcccgc cggagcgccg cgcctggacg cccccgatga gtgagccctg
      301 ggaactgtcc ctgagcgagg ccgcctccgc gatcgccgcg ggccgcctgt cgccggtcga
      361 cctggtcgaa tccacccttg accggatcga gcgcgtggag ccgcgcgtgc aggcctgggc
      421 gcgcctgcgg accgaggacg cgcggcggga ggccgggcgg ctggcgcagc tccggcgcgc
      481 ggggacgatc ctgggcccgc tccacgggat tccgatcggc gtcaaggaca tcttcaacac
      541 cgcgggggtg gagaccgcgt gcggatcgag gatcatggcg ggattcgttc ccgccgccga
      601 cgcgaccgcc gtggcccgcc tccgcgcatc cggcgcgatc atcctcggca agacccacac
      661 gaccgagttt gcttcgttcg atccctcgcc cgcccgaaac ccctgggccc tcgaccacac
      721 gcccggcgga tcgagcagcg gatccggcgc agcgctgtcg gcccggatgt gccagggcgc
      781 gttgggcagt cagacgtcgg ggtcgatcat ccgcccgtcg gcgttctgcg ggatcgtggg
      841 gctcaagccg acgttcggcc gggtgagccg gcacggcgtg caccccctgg cctggacgct
      901 cgaccacccc ggaccgatgg cgcgcacggt gcgggacgtc gccgtcttgc tcgaggcggt
      961 ggccggaccc gatgcacacg atccggcgac cctttcggct ccgccgccgc cccagtacgc
     1021 cgccctcctc gacgaacagc cggacgcggt ggcgggactc cgcgtcggga tcccggatcg
     1081 gtactttacc gatggggtcg accccgaggc ggaggagttg taccgaaccg cgcttcgagt
     1141 gctcgagacg gtggggtgcg ccgtccgcga gatccggctg ccgccggagt tcgaagccgg
     1201 gatggaagcg cacgaactga ttcacagcgc cgaagccgcc gcggtgcacc tcgaccggta
     1261 ccgcgcgcgc ccggacgact acagcagcaa gctccgcgcc atcatcgaga cggggctcca
     1321 gatcccggct ccaacctatg tgcgggcaca gcagatccgc accgtcgtga tccgtgggct
     1381 gcgagcggtc gtgcgtgacg tcgacgtgat cgcgaccccc gccgcgctcg gcccggcgcc
     1441 ggaagggctg gcctccacgg gatcgcccgt cctcaaccgg ccgttcagct tcacgggatt
     1501 tccgagcctg accgtcccct gcgggttcac cgaagcgggg cttcccgtcg gggtgcagct
     1561 ggccggtcga ccgttcgacg aggcgaccct cttgagaatc gcggcggcct acgagcacgc
     1621 caccgggtgg gggcggcgcg ttccaccgct ctgatgctca ccccgcctcc ccccggcgcg
     1681 aggcaacagg gacgcgggat ggggagcggg gcgcggcggc gggtcaggcc ctgccggagg
     1741 gggacgcctg cccccagatc ctcaggagca cgaagagcat ggccaggagg tcggccaggt
     1801 ccagggtcgg gcttctcagc acccggacca gcatgagggc agcggcgacc acggccgccg
     1861 agagcgccac cttcctgccg atctcgccga ccctgtccag gcggggcccg gcgctgcctc
     1921 ccggctgtcc ccacgggccg cg
//
LOCUS       DASQGH010000083         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3137, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000083 DASQGH010000000
VERSION     DASQGH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VEW91_01620"
     CDS             <1..2181
                     /locus_tag="VEW91_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM90313.1"
                     /translation="AVFTGRDLQGIINPIPTAWLIPNSDLKTPPHPALAVETVRYVGD
                     GVAVVVAEDPYAARDAAALVRVEYERLPAVVDQQKAVASGAPQVHADVPNNLTFTWPL
                     KGGDAAAAFAAAERDGVVIKQRFVNQRLIPNAIEPRGVVASFNSGSGDLTVWYTTQNP
                     HIARFLMSVVTGIPEHKIRIVAPEVGGGFGSKIPFYADEAVIAFCARATGRPVKWTED
                     RRENYQATTHGRDHITDLEIAARRDGTITAIRGKTWANLGAYLSTAAPGIPTILHGLM
                     LTGCYTIPNIDYTVYGVLTHTTPVDAYRGAGRPEATYLIERMVDLVAAKLNRDPVEVR
                     RKNFIPSGQFPYTTAEGLSYDSGDYGPALDRALQMVGYQRLREEQKRGPRNGRHLGIG
                     LSTYVEICGLGPSAVAGAVGFQGGLWESAIVRLHPTAKATVFTGASPHGQGEETTFAQ
                     IVADELGLPIDDIEVVHGDTAAVPMGWGTYGSRTTVVGGAAIALAARRVREKAAKIAA
                     HLLEASEQDLVFDQGRFSVRGSPDRAKTIQEVTLQAYLAWNLPPGVEPALEASAFFDP
                     SNFTFPFGTHIAVVEVDAETGQVDLKRYIAVDDCGRVINPLIVDGQVHGGVVQGIAQA
                     LYEGAAYGPDGQLLTSSMADYPVPRAHMFPPFETARTETPSPHNPLGVKGVGETGTIA
                     STAAVVNAVVDALRPYGITHLDMPLTPERIWNATHGKPREVKVP"
     gene            2178..>2584
                     /locus_tag="VEW91_01625"
     CDS             2178..>2584
                     /locus_tag="VEW91_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYM90314.1"
                     /translation="MIPAAFEYFAPQSVGEAIGLLEKHGDDAKLLAGGHSLLPIMKLR
                     LAQPKVIVDIGRIHGLDGIKADGQKIAIGALVTHDAIEHSALLKEKCPLLPEAAALIG
                     DMQVRNRGTIGGSMAHADPAADYPAAVLALDAE"
BASE COUNT          427 a          897 c          909 g          351 t
ORIGIN      
        1 gccgtcttca ccgggcggga tctccagggc atcatcaacc cgatcccgac cgcgtggctc
       61 atccccaact ccgacttgaa gaccccgccg cacccggccc tggcggtgga gaccgtgcgc
      121 tacgtggggg acggcgtggc cgtcgtggtc gccgaggacc cgtacgccgc gcgtgacgcc
      181 gccgcgttgg tccgcgtgga atacgagcgc ctgcccgcgg tcgtcgacca gcagaaggcg
      241 gtggcctccg gcgcgccgca ggtgcacgcg gatgtcccga acaacctgac gttcacctgg
      301 ccgctcaaag gcggcgacgc ggcggccgcg ttcgcggcgg ccgagcgcga cggggtggtc
      361 atcaagcagc ggttcgtgaa ccagcggctc atccccaacg cgatcgagcc ccgcggcgtc
      421 gtcgccagct tcaacagcgg ctccggcgac ctcaccgtgt ggtacacgac gcagaacccg
      481 catatcgccc ggttcctcat gtcggtggtg acgggcatcc cggagcacaa gatccggatc
      541 gtcgccccgg aagtcggggg cgggttcggc agcaagattc cgttttatgc ggatgaagcc
      601 gtcatcgcgt tctgcgcgcg ggcgaccgga cggccggtca agtggaccga ggaccgccgg
      661 gaaaactacc aggcgacgac ccacggccgc gatcacatca ccgatctgga gatcgccgcg
      721 cgccgcgacg ggacgatcac ggcgatccga gggaagacct gggcgaatct cggcgcgtac
      781 ctgtccaccg cggcgccggg gatcccgacc atcctgcatg ggttgatgct gaccggctgc
      841 tacacgatcc ccaacatcga ctacaccgtc tacggggtct tgacccacac gaccccggtc
      901 gacgcgtacc ggggcgcggg ccggcccgag gcgacctacc tcatcgagcg gatggtcgac
      961 ctggtggcgg ccaagctcaa ccgggatccc gtcgaggtcc ggcggaagaa cttcattccg
     1021 tccggacagt tcccctacac caccgccgag gggttgtcct acgacagcgg ggactacggc
     1081 ccggccctcg atcgggcgct ccagatggtc gggtaccagc gcctgcgcga ggagcagaag
     1141 cgcggccccc ggaacggcag gcacctgggg atcgggctca gcacctatgt cgagatctgc
     1201 ggcctggggc cctctgcggt ggccggggcg gtggggttcc agggcgggct gtgggagagc
     1261 gcgatcgtcc ggctgcaccc gaccgcgaag gccacggtgt tcacgggcgc atcgccgcac
     1321 ggccaggggg aagagacgac gttcgcccaa atcgtcgccg acgagctcgg gctgccgatc
     1381 gacgacatcg aggtcgtgca cggcgacacc gcagcggtgc cgatggggtg ggggacctac
     1441 ggcagccgca cgaccgtcgt cggcggcgcg gcgatcgcgc tggccgcccg ccgcgtccgc
     1501 gagaaggccg cgaagatcgc cgcccacctc ctggaggcct ccgagcagga cctcgtgttc
     1561 gatcaggggc ggttttccgt ccgggggagc cccgaccgcg ccaagacgat ccaggaggtg
     1621 accctccagg cgtatctcgc gtggaacctg ccgcccgggg tggagccggc gctggaagcc
     1681 tcggcgttct tcgatccctc caacttcacg tttccgttcg ggacgcacat cgcggtcgtt
     1741 gaggtggacg cggagaccgg gcaggtcgac ctcaagcgct acatcgccgt ggacgactgc
     1801 ggccgggtga tcaacccgct gatcgtcgac ggccaggtgc acggcggcgt ggtgcagggg
     1861 atcgcgcagg cgctgtacga gggcgcggcg tacgggccgg acgggcagct gctcaccagc
     1921 agcatggccg actacccggt gcccagggcg cacatgttcc cgcccttcga gacggcgcgc
     1981 acggagacgc cctcgccgca caacccgctc ggcgtcaagg gggtcggcga gacgggcacg
     2041 atcgccagca cggcggcggt cgtgaatgcg gtcgtggacg ccctccgtcc ctacggcatc
     2101 acgcacctcg acatgccgct cacaccggaa cggatctgga acgcgaccca cggtaagccc
     2161 cgggaggtga aggtaccgtg attcccgcag cgtttgagta cttcgcgcct cagtccgtcg
     2221 gcgaggcgat cgggctcttg gagaagcacg gggacgatgc gaagctcctg gccgggggcc
     2281 acagcctcct cccgatcatg aagctccggc tggcgcagcc caaggtcatc gtcgacatcg
     2341 gccggatcca cgggctggac gggatcaaag ccgacgggca gaagatcgcg atcggcgcgc
     2401 tggtgaccca cgacgccatc gagcacagcg ccctgctcaa ggagaaatgc cccctgctgc
     2461 ccgaggcggc cgcattgatc ggcgacatgc aggtccgaaa ccgggggacc atcggtggaa
     2521 gcatggccca cgccgacccc gccgcggact atcccgcggc ggtgctcgcg ctcgacgcgg
     2581 agat
//
LOCUS       DASQGH010000084         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11688, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000084 DASQGH010000000
VERSION     DASQGH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11688"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VEW91_01630"
     CDS             complement(<1..464)
                     /locus_tag="VEW91_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90315.1"
                     /translation="MGARHRADQVGSLLRPPELLEARLASAQNSASTPDPERLPAIED
                     RHILRVLARQQELGFDVFTDGELRRRNFMSDFTDAVEGFDLGDAVARPWQAGSAAGAP
                     VSSVTGIVTAKLRQLRRLTAHELPFLRAHSPGTFKMTLPSATQFPAIAYKRGV"
     gene            606..>1899
                     /locus_tag="VEW91_01635"
     CDS             606..>1899
                     /locus_tag="VEW91_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90316.1"
                     /translation="MEPKTEAAARMMKQMGLTIKASALYPPTHPGNALAVETLLHVLS
                     AYIQMYGQFSVGVGKHALSIDGTAVPGGASAALAYSLYSRKLVQFTVHPTVSQPQLAS
                     FVRIVGMERTKLEEAGGIAHLLSESGVTDITVKELALGADHEVEILGREAFLSLAGRG
                     RLSPEEGEQVIDTVRGGPEGASRLLQGAYALAGETLDRVTVEGQLKQVVQATKSVDRL
                     ILNEPFDQQGALYTNLAQALLLLEKPLGPSLVRNLLSKAGEDITAQVILEHLSSEQLA
                     KIVLKSLEEGDVVEQITRTMRGWTLDQEKAKNVLALLDSHLREQGEGGAVLTETVQRE
                     LRPLPPETADEVPPAIEFDDGQVAVSDEELKRCLREAHAIDEAGSVREVVKTFVDVLA
                     NALEKEELVDVAEALAGYLTGVVDRQEFALLREALERVK"
BASE COUNT          329 a          610 c          642 g          318 t
ORIGIN      
        1 acgccccgct tgtacgcgat cgcggggaac tgggtggcgc tcgggagggt catcttgaag
       61 gtgcccgggc tgtgcgcccg caggaacggc aactcgtgcg cggtcaggcg ccggagctgt
      121 cggagtttgg cggtgacgat cccggtcacg ctgctcaccg gtgcgccggc ggcacttccg
      181 gcctgccacg gtcgggcaac cgcgtccccg aggtcgaagc cttcgaccgc atccgtgaag
      241 tcgctcatga agttgcgccg acgcaactcg ccgtcggtga agacgtcgaa ccccaactcc
      301 tgttgcctgg cgaggacgcg gaggatgtga cggtcttcga tcgccgggag gcgctcggga
      361 tcgggcgtgg aggccgagtt ctgcgcactt gccaggcggg cttccaatag ctcggggggg
      421 cgcagcaggc tacccacctg gtcggctcgg tgacgtgcgc ccatgtgctc ctcctccaac
      481 acggtgctcc cggcttcggc cggcctcttc gggttcctgc gcggagtctc ctttcctccg
      541 gcgttcgagc gtgggggtct gggtgcggac ggaagagggc atgaaatagg cgcggggacc
      601 cgacgatgga gcccaagaca gaggctgcgg cccggatgat gaaacagatg ggcttgacca
      661 tcaaagcctc tgccctctat ccgccaacac accccggaaa cgccctggcc gttgaaaccc
      721 ttctccacgt gctgtccgcc tatatacaga tgtacgggca attctctgtg ggcgtgggga
      781 agcacgccct ctccatagat gggaccgccg tccccggcgg cgcgtccgcc gctctggcgt
      841 actccctcta cagtcgcaag ctcgtccagt tcaccgtcca ccccacggtc agccagccgc
      901 agcttgcttc gttcgtcagg attgtcggta tggaacgcac caaactcgag gaggcgggtg
      961 ggattgcgca cctgctctcc gaatcgggcg tcacggatat cacagtgaaa gaactggccc
     1021 tgggggccga tcatgaggtc gagatcctcg gccgggaagc cttcctcagc ctcgcggggc
     1081 gaggcaggct gagccccgaa gagggggagc aggtgattga taccgtgcgc ggcggaccgg
     1141 aaggggccag caggcttctg caaggcgcct acgcgctggc cggggagacc ctcgaccggg
     1201 tcaccgtgga agggcagctc aagcaggtcg tccaggcgac caagtccgtt gaccggctca
     1261 tcctcaacga acccttcgac cagcaggggg cgttgtacac caacctcgcc caggccctgc
     1321 tcctgctgga aaaaccgctt ggtccctctc tggtgcgcaa cctgctctcc aaggctggcg
     1381 aggatatcac agcccaggtc atcctggagc acctctcgag cgagcaactc gccaagatcg
     1441 tcctcaaatc gttggaggag ggggacgtgg tcgagcagat cacccgcacc atgcgggggt
     1501 ggacgctcga ccaagagaag gccaagaatg tgctcgcgct cctcgattcg cacctccgcg
     1561 agcagggtga gggcggagcc gtgctcaccg agacggtgca acgtgagctt cgtcccctgc
     1621 ctcccgaaac cgctgacgag gttccgcctg cgatcgagtt cgacgacggc caggtcgcgg
     1681 tctccgatga ggagctcaag cgctgcctga gagaggcgca cgcgatcgat gaggccggat
     1741 ccgtccgcga agtcgtcaag acctttgtcg acgtcttggc caacgcgctc gagaaagagg
     1801 aactggtcga tgtggccgag gccctcgccg gctacctgac cggggtggtg gaccgccagg
     1861 agttcgcgct cttgcgcgag gcgctggagc gggtcaagg
//
LOCUS       DASQGH010000085         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48463, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000085 DASQGH010000000
VERSION     DASQGH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48463"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="VEW91_01640"
     CDS             <1..2080
                     /locus_tag="VEW91_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYM90317.1"
                     /translation="GADLERVRSQVVYLLGEEGTASYTKQASKTPTLDEFGRDLTKLA
                     RDNKLDPVIGREREIERVIQVLSRRTKNNPALIGEPGVGKTAITEGLAQRIVRGDVPE
                     VLRNKRVVQLDLAALVAGTKYRGEFEERMKKVMEEIRKAQGEVVLFVDELHTLVGAGA
                     AEGAIDASNILKPSLSRGELQCIGATTLDEYRKYVERDAALERRFAPILVAEPNVDQT
                     VEILRGLRERYEAHHGVKISDEALVAASTLADKYISDRFLPDKAIDLMDEAASKIRLQ
                     ASFLPQEVRQALDKAERARREKEEAIKGQDFEKAASLRDKEKVLRQKLEELESSWKTD
                     KGRDITTVTADDIADIVSSWTGIPVMRLVEEETEKLLRMEDSIHSRIVGQEEAVHAVS
                     RAVRRARAGLKDARRPIGSFIFLGPTGVGKTELTLALAEFLFGDEGAVIRIDMSEYTE
                     RHTVSRLVGAPPGYVGYEEGGQLTEQVRRRPYSVVLLDEIEKAHPEIFNVLLQILEDG
                     RLTDAQGRTVDFKNCVVIMTSNVGAPQIQRESGFGFRGGEGERQEAEAAYDRMKTHVM
                     EELRRTFRPEFLNRVDEIIVFHPLTREQIRLIVDILMERIKREIRGQGMTLAMTDPAR
                     DLLAAEGYDPQYGARPLRRAIQRMVEDPLSDDMLRGKFRAGDEVVLDVRQGVVVFEKK
                     REPVSADTGA"
BASE COUNT          390 a          644 c          770 g          300 t
ORIGIN      
        1 gggcgccgac ctggagcgcg tgcgctccca ggtggtctac ctcctgggcg aggagggcac
       61 cgcctcgtac accaagcagg ccagcaagac ccccacgctg gatgagtttg ggcgggatct
      121 caccaaactc gcccgcgaca acaagctcga tccggtgatc ggccgggagc gcgagatcga
      181 acgggtcatc caggtgctct cccggcgcac caagaacaac cccgcgctga tcggcgaacc
      241 cggggtgggc aagacggcga tcaccgaggg gctcgcccag cggatcgtcc ggggcgacgt
      301 tcccgaggtc ctgcggaata agcgggtcgt ccagctcgac ctggccgcgc tcgtcgccgg
      361 gacgaagtac cggggcgagt tcgaagagcg catgaaaaag gtgatggagg agatccgcaa
      421 ggcgcagggc gaggtcgtcc tcttcgtgga cgagttgcac accctggtgg gggccggcgc
      481 cgccgagggc gcgatcgacg ccagcaacat tctgaagccc tcgctgtccc ggggagagtt
      541 gcagtgcatc ggggcgacca cgctcgacga gtaccggaag tatgtggagc gggacgcggc
      601 gctcgagcgg cggtttgcgc cgatcctcgt ggccgagccc aacgtcgatc agacggtgga
      661 gatcctgcgt ggcctgcgcg agcggtacga ggcccaccac ggggtgaaga tcagcgacga
      721 ggcgctcgtg gccgcctcca cgctcgccga caagtacatc tccgatcggt tcctgccgga
      781 caaagcgatc gacctgatgg acgaggcggc gagcaagatc cgcctccagg cgagcttcct
      841 gccgcaggag gtccgccagg cgctcgacaa ggccgagcgg gcgcggcggg agaaggaaga
      901 ggcgatcaag ggtcaggact tcgagaaggc cgccagcctc cgcgacaagg agaaggtgct
      961 gcggcagaaa ctcgaggaac tggagagttc ctggaaaacc gataagggcc gggacatcac
     1021 gaccgtgacc gcggacgata tcgcggacat cgtgtcgagc tggaccggga tcccggtgat
     1081 gcggctggtg gaggaggaga ccgagaagct cctccggatg gaagactcca tccattcgag
     1141 gatcgtcggg caggaagaag ccgtccacgc ggtctcccgt gcggtccggc gcgcgcgggc
     1201 gggcctgaag gacgcccggc ggccgatcgg atcgttcatc ttcctcgggc ccaccggcgt
     1261 gggcaagacc gagctcaccc tggcgctggc ggagttcctg tttggcgacg agggggcggt
     1321 catccggatc gacatgtcgg agtacaccga gcgccacacc gtctcgcggc tcgtcggggc
     1381 gcctccgggc tatgtggggt acgaggaggg cggccaactc accgaacagg tccgtcggcg
     1441 accgtactcg gtggtcctcc tcgacgagat cgagaaggcg catcccgaga tcttcaatgt
     1501 gctgctgcag atcctggagg acggccgtct gaccgatgcg caggggcgga cggtcgactt
     1561 caagaactgc gtcgtcatca tgaccagtaa cgtcggcgct ccccagatcc agcgggaatc
     1621 cggattcggg ttccgcggcg gggagggcga acggcaggaa gccgaggccg cgtacgaccg
     1681 gatgaagacc catgtgatgg aggagttgcg gcggacgttt cgcccggagt tcctgaaccg
     1741 ggtcgatgag atcatcgtct tccacccgct gacgcgcgag cagatccggt tgatcgtcga
     1801 catcctgatg gagcggatca agcgcgagat ccggggccag ggcatgacgc tggcgatgac
     1861 cgatccggcg cgcgacctcc tcgcggccga gggatacgat ccccagtacg gggcgcggcc
     1921 gctgcggcgg gcgatccagc ggatggtcga ggatcccctc tcggacgaca tgctgcgcgg
     1981 caagttcagg gcgggcgacg aggtcgtctt ggatgtccgc caaggggtcg tggtgttcga
     2041 gaagaagcgc gaacccgtct cggccgatac cggggcgtaa cgccctgcat cggccggtgg
     2101 agcg
//
LOCUS       DASQGH010000086         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8875, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000086 DASQGH010000000
VERSION     DASQGH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8875"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VEW91_01645"
     CDS             <1..815
                     /locus_tag="VEW91_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90318.1"
                     /translation="ISRVPLPQVKSLTQEAAQAGARFQLLQHGAFRWDGITLNATKPP
                     FDNKLLRQAFDATIDREAIANVVLQGAAYPAYSFFPTGTPAYDPTWKLPPRNIGLAKE
                     KLQAAGQPNGFTFTLLTGAGQQDQAVAQAVQAMAADAGIQVRLQIIEGGELLDEMTHL
                     RHQAALIFWSGRPDPDFDIYPFVTQSGIGALNFSGYTNQRVQILLDAARLLNDMPQRR
                     RAYGEVTRIVADDVPYVLLYFPKEYKLLSNRVHGFTLVPDGMLRLRDVWLSP"
     gene            1159..2172
                     /locus_tag="VEW91_01650"
     CDS             1159..2172
                     /locus_tag="VEW91_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYM90319.1"
                     /translation="MRHRNLGKTGLQVSALGFGTGAIGGLFVRGDATEQRRAVARALE
                     AGITYFDTAPSYGDGRSEENLGRVLRDLDAWGRVVVGTKVRLEAGDLPNPHAAILRSL
                     EGSLRRLGRGAVDLLHLHNPISGEGGAGAIETASGNALNLDTVLGSVAEGMQQVVRRG
                     LARHIGITGLGNAAALHAVVAAAGFETIQTYFNILNPSAGFAGASGGAQDFAGVIDKA
                     AEAGMGVIAIRVMAAGAISARPARHANAGDPGAPLVSGAAYGRDLERARAFELLATEL
                     GTAGPLELALRFALAKPGVSTVLVGYSDLPQLEDAIRWTERGPLSADVVRRLVDTARS
                     VKP"
     gene            complement(2179..3339)
                     /locus_tag="VEW91_01655"
     CDS             complement(2179..3339)
                     /locus_tag="VEW91_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYM90320.1"
                     /translation="MLRRLSRARRDAVLYGAEHAVLLEIINDLHQSLQTALDGRPSVK
                     LFIHEDTFFIDNKVLLEESLQLDALLKAFKDREINAVQVNAGVESWELKHLIGVLNRK
                     AEQFGRPGAAQAYLEEGGVKHIKVGFAAPSETRSTPAGMVVDPQDIYRAGLRVMDELT
                     YQAGMNLPLNVGKALKVVNYLLDVAADDSAALFGLTGMKNYDEDTYHHSVRVSMLSLL
                     IGSQLNLDRAVLVTLGLAGLLHDIGKMRIPRELIASSAKLTPEEEGMVRRHTVHGAHA
                     LRDLSGLLRLALVVAFEHHANYNLSGYPRITMKEVPHLLSRIVQVADVFDAATSSRRA
                     YRRSKRPAEALKAILDGAGTEFDPVLARFSVKVLAGLFREATRRAQAAQPHD"
BASE COUNT          591 a         1144 c         1208 g          594 t
ORIGIN      
        1 tcatcagccg cgtgccgctt ccacaagtca agtcgctgac ccaggaggcg gcgcaggccg
       61 gggcgcggtt ccaactcctc cagcacggcg cgttccggtg ggatgggatc accctgaacg
      121 ccaccaagcc tccgttcgac aacaagctgt tgcgccaggc cttcgatgcg acgatcgatc
      181 gggaggcgat cgcgaacgtc gtcctgcagg gcgccgccta ccctgcctac tcgttctttc
      241 ccacgggaac gccggcgtac gatccaacgt ggaagctgcc cccacgcaac atcgggctcg
      301 ccaaggagaa gctgcaggcc gccggacagc cgaacgggtt cacgttcacc ctgctcaccg
      361 gggccggcca acaggatcag gcggtcgccc aggcggtcca ggccatggcc gccgatgcgg
      421 ggatccaggt caggctccag atcatcgagg gaggcgagtt actcgacgag atgacccacc
      481 tccggcacca ggcggcgctc attttctgga gcggccgccc ggacccggat tttgacatct
      541 accccttcgt gacgcagtcc ggcatcgggg cgctcaactt ttccgggtat acaaaccaga
      601 gggtccagat cctgctggac gcggcgcggc tgctcaacga catgccccag cggcgccgcg
      661 cgtacggcga ggtgacccgg atcgtggcgg acgatgtgcc ctacgtcctc ctctattttc
      721 ccaaggagta caagctactc agtaaccgcg ttcacgggtt tacgcttgtt cccgacggca
      781 tgctgcggtt gcgggatgtc tggctgtccc cctgagcgcg gtcgacggtt acccaccggg
      841 gtgggcgata tcgcgttcgc cggttgcgaa ctggcgcgcc cgttgctcga tctcggtcgc
      901 ggtcacgagg ccgcattcca ccagcagctt ctgcagggca gccagccagc tctcgtagta
      961 ccccggcctc gcgtcgctgg tgaccgagcc gggcccggtc ccctttgagg ttgaggatat
     1021 tgaacgtcgg gacgggcttt aggagccttc cgaggaaacc tgcggggcct gttgaactcc
     1081 gaagacgcaa gcaaaggccc ggtgccgggg acccgtttcc cccgccttaa ccgaggcgtt
     1141 tgcttcagga ggcacccgat gcgccaccgg aacctcggaa agacgggatt gcaggtctcg
     1201 gcgctcggct ttgggaccgg cgcgatcggc ggcttgttcg tccgcggcga tgccaccgag
     1261 cagcggcgcg ccgtcgcgcg ggcgctcgaa gcgggaatca cctacttcga caccgcgccg
     1321 agctatgggg acggacgttc ggaggagaac ctggggcgcg tcctgcgcga cctggacgcc
     1381 tggggccgcg tggtggtggg gacaaaggtg cggctcgagg ccggcgacct ccccaacccc
     1441 cacgcggcca ttctgcgatc gctcgagggg agcctgcggc ggctggggcg cggagcggtc
     1501 gacctgctgc acctccacaa cccgatttcc ggcgagggcg gcgccggcgc gattgaaacc
     1561 gcgagcggga acgcgctgaa tctcgacacc gtgctgggaa gcgttgccga agggatgcaa
     1621 caggtggtgc ggcgcgggct ggcgcgccac atcggcatca ccgggttggg gaatgccgcg
     1681 gcactgcacg cggtcgtggc cgcagccggg ttcgagacga tccagaccta tttcaatatc
     1741 ctgaacccga gcgcggggtt cgcgggagcg agcgggggcg cgcaggactt cgcaggagtg
     1801 atcgacaaag ccgccgaggc ggggatggga gtgatcgcca tcagggtcat ggcggcgggg
     1861 gcgatttcgg ccaggccggc acggcatgcc aatgcgggag accccggcgc accgctggtg
     1921 agcggtgccg catacggccg cgaccttgag cgggcgcgcg ctttcgaact gctcgccacg
     1981 gaattgggaa ccgcaggacc cctggagctg gcattgcgct tcgcgctcgc caagccaggg
     2041 gtttcaaccg tcctcgtggg gtactcggac cttccccaat tggaggacgc aatccgttgg
     2101 acggagcgcg ggccgctttc ggcggacgtc gtccggcgtc tcgtggatac ggcgcgaagc
     2161 gtcaagccat agagcccgct agtcgtgcgg ctgagcagcc tgcgcccggc gggtggcctc
     2221 gcgaaacagg cccgccagga ccttcacgga gaatcttgcc agcacaggat cgaactctgt
     2281 ccccgccccg tccaggatgg ctttgagggc ctctgcggga cgcttggacc gacgatacgc
     2341 tcgccgcgag gaggtcgcgg cgtcaaagac gtcggccacc tgcacaatgc gcgagagcag
     2401 gtggggcacc tccttcattg tgatccgcgg gtagcccgaa agattgtaat tcgcgtggtg
     2461 ctcgaaggcc accacgaggg ccagcctgag gaggcccgag aggtcgcgca gggcgtgagc
     2521 accatggacc gtatggcgcc tcaccatccc ctcctcttcg ggcgtaagct ttgcggagct
     2581 ggcgatgagc tctcgaggga tccgcatctt gccgatatcg tggaggagcc cggcgaggcc
     2641 caacgtgacg agcactgccc gatccaggtt caactgggag ccgatcaaca gtgagagcat
     2701 gctgaccctc accgagtgat ggtaggtatc ctcgtcgtaa ttcttcatac cggtgagccc
     2761 gaacagggcg gcactatcat cggcggcgac gtcgaggagg tagttgacca ccttgagcgc
     2821 tttccccacg ttgagcggca ggttcatccc tgcctggtag gtcaactcat ccatgacccg
     2881 cagcccggcg cggtagatat cctggggatc cacgaccatc cccgcgggtg tcgacctggt
     2941 ttcggacggt gcggcaaacc caaccttgat gtgcttgacc ccgccctcct ccaggtaggc
     3001 ctgcgcggcc cctgggcgcc cgaactgttc ggccttccgg ttgagaaccc cgatgagatg
     3061 tttcagctcc caggactcca cgccggcgtt cacctgcacg gcgttgatct cccggtcctt
     3121 gaatgccttg aggagcgcgt ccagctgcag gctttcctcc agcaagacct tgttgtctat
     3181 gaagaaggtg tcctcgtgga tgaagagctt gaccgagggg cggccgtcca gggctgtttg
     3241 caaggactgg tgaagatcgt tgatgatctc gaggaggacc gcgtgctctg caccgtagag
     3301 gacggcgtct cgccgggcgc gggagagccg tctcagcatc tctcccggcc tttctgggtt
     3361 ggggcgcgtc ggagctccgg cgtcacgccg ccccagcgcg ggcgcgtccg agcctaccgg
     3421 cgaagcggag gggtcgctca tggaaccttc tttccctcga tgctggcgac ggccgcgcgg
     3481 gcaagccggc gaagctcacg gccatgccgg tgaaagacga ggtgggggcg cgcaagt
//
LOCUS       DASQGH010000087         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8876, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000087 DASQGH010000000
VERSION     DASQGH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8876"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VEW91_01660"
     CDS             <1..435
                     /locus_tag="VEW91_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYM90321.1"
                     /translation="VRRRGGVGRVVMKIHRLLSHAEKARLRHLVEAVERETGAEIATL
                     IVRHVDDVEAFATAYFNHLGIGKREHHNGVLILAVLDRRRVRIEVGRGLDRVLTRAAA
                     QRIIDEAMAPAFRVGRYGEGLIQGVQAVGALIRAAHAGAGKA"
     gene            432..1007
                     /locus_tag="VEW91_01665"
     CDS             432..1007
                     /locus_tag="VEW91_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYM90322.1"
                     /translation="MRPLAIFVLGAATAAAGFGWSTATYNRLVQARLAVDAQWAQVEA
                     QYQRRVDLVPGLIEAVKGVLAQERTLLEELARARAAYLSAPAGSLGRVEAANALERPL
                     GRLVGVVEAYPQLRSADAVARLMDELAGTENRIAVERRRYNERVRIYNTLVQQFPSSL
                     VAALGHFRPRAYFNAAPPSGAAPPGVNLPPP"
     gene            1123..1869
                     /gene="lipB"
                     /locus_tag="VEW91_01670"
     CDS             1123..1869
                     /gene="lipB"
                     /locus_tag="VEW91_01670"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYM90323.1"
                     /translation="MITEAGRWIVAGAARARTAWLVECGRMPYADAWGLQRALVTARQ
                     AGRIEDVILLVEHPPVVTIGRGGRAANILVSRDALAARGFEVHEIERGGDVTYHGPGQ
                     LVGYPILDLRALDEDVVRYVRLLEAALIGALDRFGIAGARLRGYPGVWVGDAKIAAIG
                     VAVKRKVTMHGFALNVAPDLDHFEVINPCGLGKPVTSMARLLGRPVSLAEVQPAVAWS
                     FDEVFEIALQPIAVDALRARLGEAITPGTG"
     gene            1871..2827
                     /locus_tag="VEW91_01675"
     CDS             1871..2827
                     /locus_tag="VEW91_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HYM90324.1"
                     /translation="MTVQTLGVGPLRAAPGTKVTGFLEVPGTPIAMPVTLVHGTAPGP
                     VLSVTAGVHGGEYPGIEAAIRTAAALDPAGIRGSVIIVHVVDVPAFHGRNIYVCPLDG
                     KNPNRVFPGNPAGTASERLAHTVFTEVIARADFYVDLHGGDLNEALVPFTILLESGDP
                     AVDGRALGLARVYGIKYVVRGRVAGGTYAAAAKHGIPAILTEAGGQGLLDEQAVHVHL
                     QGLRNVLTHIGITPGDPDPVEPITLLSGFHWVTSEDLGLCYLGVAPGARVTQGQPVGE
                     VRDYFGRRLRTVLAPASGIVLFTVTTPATSPGDPVFAVGTPG"
     gene            complement(2906..3268)
                     /locus_tag="VEW91_01680"
     CDS             complement(2906..3268)
                     /locus_tag="VEW91_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90325.1"
                     /translation="MKRVRRRPAVRAKRRAVRGKRRAVRATRRGVRATRRGVRAKRRA
                     VRATRRGVRAKRPVLRRAAARKPAPSPLGQMPLGSYTTPQPISPLEPMRPDPWDRPAE
                     HHPHEESGMEDEEDEEPL"
     gene            3589..>4476
                     /locus_tag="VEW91_01685"
     CDS             3589..>4476
                     /locus_tag="VEW91_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90326.1"
                     /translation="MRERTGTFWVTILIATAVLVTTLPVVGSSTQPAGKLSSPQGGSK
                     AAAASPGSSGVGAGSPSVLLSRVQEQLSWRRRRLAQVVQQERWALAQLSGAQERLERA
                     VAHLNQTTSALTSAHRAVQSATHVLQGVTTRLAAHEELMGARIRAFYERGPVGYLDML
                     LGAADFQDFLARSYLITRIIERDLALYHAVAAERQQQEDVRASLVSHQETLSEEQEQW
                     LARRQETVRLAAERRRLLDRVRTERQTQEAAIRELEAESARIMEIIRKSARGGRRGPI
                     LTLRNGALLRPVPGPITSGY"
BASE COUNT          633 a         1524 c         1606 g          713 t
ORIGIN      
        1 gtcaggcggc gggggggcgt cgggagggtg gtaatgaaga tccacaggct cctgagccac
       61 gctgaaaagg cccggctccg ccacctggtg gaggcggtgg agcgtgagac cggggcggag
      121 atcgcgaccc tcatcgtccg gcacgtggac gatgtcgagg cctttgcgac cgcgtacttc
      181 aaccacctcg gcatcggaaa acgggagcac cacaacggag tcctgatcct cgccgtcctc
      241 gaccgccggc gggtccgcat cgaggtgggg cgcgggcttg atcgggtgct cacgcgcgct
      301 gcggcccagc ggatcatcga cgaggccatg gcgccggcgt tccgggtcgg ccgctacgga
      361 gaaggactca tccagggcgt ccaagccgtt ggagcgctga tccgcgcggc gcacgccggc
      421 gccgggaaag cgtgagaccg ctcgccatct ttgtcctcgg cgcagccacc gctgcggccg
      481 ggtttggctg gtcgaccgca acgtacaacc gtcttgtgca ggcgcggctc gccgtcgatg
      541 cccagtgggc tcaggtcgag gcccagtatc agcggcgcgt ggacctggtt ccgggcctca
      601 tcgaggcggt gaagggcgtc ctggcccagg agcgcaccct gctggaggaa ctggcgcggg
      661 cgcgcgccgc atacctgtcc gcccccgccg ggtcgctggg ccgcgtcgag gcggcgaacg
      721 cccttgagcg gcccctcggg cgcctggtgg gcgtggtgga ggcgtacccg caactacgga
      781 gtgctgacgc tgtcgcccgg ctcatggatg aactggcagg gacggagaac cgcattgctg
      841 tggagcggcg ccggtacaac gagcgggtga ggatctacaa caccctggtg cagcagtttc
      901 cgagctcgct cgtcgccgca ctcgggcact tccggccgcg cgcatacttc aacgcggcac
      961 ccccgtccgg tgcggcacca ccgggcgtca accttccccc tccgtagagg cagtaccctc
     1021 cctaagcgcg cggcttggtt ggggtccgtc ttgacagggt gccgggggct aggctagcct
     1081 aacccgtgaa agatttctga tagttagagt gaggaacagc cggtgatcac ggaagctgga
     1141 aggtggattg tggccggagc ggcgagggcg cggacggcgt ggctcgtcga gtgcggccgg
     1201 atgccgtacg cggacgcgtg ggggctgcag cgtgcgctcg tcaccgctcg tcaggcgggc
     1261 cggatcgagg acgtcatcct gctggtcgag catccccccg tcgtcacgat cggacgcggg
     1321 ggccgtgcgg cgaacatcct ggtgtcccgg gacgcgctgg ccgcccgggg gttcgaagtc
     1381 cacgagatcg agcgcggagg cgacgtgacc taccacggtc ccgggcaact ggtggggtat
     1441 cccatcctcg atctcagagc ccttgacgag gacgtcgtcc gctacgtccg gctgctggaa
     1501 gccgctctga ttggcgcgct cgaccggttc gggatcgccg gggcgcgtct gcgcggctac
     1561 ccgggggtgt gggtcggcga cgccaagatc gccgcgatcg gcgtggccgt gaagcggaag
     1621 gtcacgatgc atgggtttgc gctcaacgtg gcgcccgacc tggaccactt cgaggtgatc
     1681 aatccgtgcg ggctggggaa gccggtcacg agcatggccc gcctgctcgg gcgccctgta
     1741 tcgctcgcgg aggttcagcc ggccgtcgcg tggtcgttcg atgaggtgtt tgagatcgcg
     1801 ctccagccga tcgccgttga cgcgctccgg gcgcgactag gcgaggccat aacaccaggg
     1861 acggggtagg atgacggtgc agacgttggg agtcggcccg cttcgggcgg cgcccgggac
     1921 caaggtgacg ggcttcctgg aggttcccgg cacccccatc gcgatgcctg tcacgttggt
     1981 gcacgggacg gcccccgggc cggtcttgag cgtgaccgcc ggcgtgcacg gcggcgagta
     2041 ccccgggatc gaggccgcga tccggaccgc cgccgcgctc gatccggcgg gcatccgggg
     2101 ctccgtgatc attgtccacg tcgttgacgt gcccgccttc catggccgca atatctacgt
     2161 ctgcccgttg gatggcaaga acccaaaccg ggtcttcccc ggcaacccgg caggaacggc
     2221 cagcgagcgg ctggcgcaca ccgtgtttac cgaggtgatt gccagggctg acttctacgt
     2281 cgacctgcac ggcggggacc tcaacgaagc cctggtgccc ttcacgatcc ttctggagtc
     2341 gggcgatcca gccgtggatg ggcgagcgct cggcctcgcg cgcgtctacg gcatcaagta
     2401 cgtcgtccgg ggccgggtcg cggggggcac gtacgcggcc gccgcaaagc atggcattcc
     2461 cgcgatcctc accgaagcgg gcggtcaggg cctgctcgac gagcaggcgg tccacgtgca
     2521 cctgcagggt cttcgcaacg tgttgaccca cattggaatc acccccggag accccgatcc
     2581 cgtcgagccc atcacgctgc tctccggctt ccactgggtg acctcggagg atttgggact
     2641 ttgctacctc ggcgttgcgc ccggtgcgcg ggtgacccag gggcagccgg tcggggaggt
     2701 gcgcgactac ttcggccgcc gcttgcggac ggtcctcgcg ccggccagcg gaatcgtgct
     2761 gttcaccgtg accaccccgg ccactagccc cggggacccc gtcttcgcag tcgggacccc
     2821 gggatagcgg cccccggaat gcgccaggcc ggcgccagag gcgccggccc gcacgctgag
     2881 tgagctccgc agccgacctg gcaggctaga gcggctcctc gtcctcctcg tcctccatcc
     2941 ccgactcctc gtgggggtgg tgctcggccg gccggtccca aggatccggc cgcatcggct
     3001 cgagcggaga tatgggctgg ggcgtggtat acgatcccag gggcatctgc cccaaaggcg
     3061 acggggcagg cttgcgagcg gcggcgcgcc tgaggaccgg tcgcttagcc cggacccccc
     3121 ggcgcgtggc ccggacggcc cggcgcttag cccggacccc ccggcgcgtg gcccggaccc
     3181 cccggcgcgt ggctcggacg gcccggcgct tgccccggac ggcccggcgc ttggcccgga
     3241 cggccgggcg cctgcgaaca cgcttcacac gcttccggga cgtcctccgg gtcccggtcc
     3301 tacgtggcgc tcgttttctc gccattcgtt gacctcccct ttcccgccga aagagatgca
     3361 ctgttccaga tcgcggtgtc cgggcgttgg ttcgacgggg cggttggttt tccttttcct
     3421 cgggtgccga gcgggggtcc tggcaggcgg cgtttcccca gcggggtgcg ggaaggagtt
     3481 tcctccaatc cgggcgaatc gcgctgtcca tgtgacccag ccgggcgtcc cggccgttta
     3541 ccgtccttat tatctatcct ggtagttgaa tcacgttcgg agcagaggat gcgggagcga
     3601 actgggactt tttgggtcac catcctcatt gcgaccgcgg tgctggtcac gaccctcccc
     3661 gtggtggggt cgtccacgca gccggctggt aagctatcct ccccccaggg tggttccaag
     3721 gccgccgccg cctccccggg gtcctccgga gtgggtgcgg gatccccgtc cgtgctgctg
     3781 tcgcgggtcc aagagcagct ttcgtggcgc cgccgccgtc tggcgcaggt cgtgcagcag
     3841 gagcggtggg ccctggccca actctccggc gcccaggaac gccttgaacg ggcggtcgcg
     3901 catctcaacc agaccacctc cgccctcacc agcgcccacc gcgccgtcca aagcgcgacc
     3961 cacgtcctcc agggggtcac gaccaggctg gcggcccacg aggaactgat gggcgcccgc
     4021 atccgcgcgt tctacgagcg aggcccggtg ggatacctgg acatgctgct cggggccgct
     4081 gacttccagg atttcctcgc ccgatcgtac ctgatcacca ggatcatcga gcgtgatctg
     4141 gccctctacc acgcggtggc ggcggagcgc cagcagcagg aggatgtgcg cgcctcgctc
     4201 gtgtcacacc aggagacgct gtccgaggaa caggagcagt ggctcgcgcg caggcaggag
     4261 accgtgcggc tcgccgccga gcggcgccgg ttgctcgacc gcgtccggac cgagcggcag
     4321 acgcaggaag cggcgattcg agaactcgaa gccgagtcgg cgcggatcat ggagatcatc
     4381 cggaagtcgg cccggggggg acgccgcggg ccgatcctga cgctgcggaa cggcgcgctt
     4441 ctccggccgg tccccggccc gatcacctcg gggtac
//
LOCUS       DASQGH010000088         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8889, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000088 DASQGH010000000
VERSION     DASQGH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8889"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..>1554)
                     /locus_tag="VEW91_01690"
     CDS             complement(55..>1554)
                     /locus_tag="VEW91_01690"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYM90327.1"
                     /translation="GAAAAAIGVAEPYMSGLAGCGTLVLTPPGEAPRVLIFLGRAPGA
                     ATPDRFPAGPPDAGYLAPAVPGNLAGWARVLAEYGKLSLAHVLEPAIELAERGIPLTP
                     FDHQMFTESGGRLTRDGVANYFHNGHVPAIGALLVQPDLARTFRQIAAEGIGYLYGGP
                     LGQAIGRLMQDGGGLLTAADLRGYPETLRWTAPIQTTYRGVTVYAPPPPTSAIQVLET
                     LNVLETFDVGNTGHLSPDHLAAVAEAARAARLDTDRYVGDPAFVEVPVARLLSAEHTR
                     ELRAQAEWRLRAPSAVAGATGAEANGADGSGASTTHLAAADASGLAVNITHSLGHGFG
                     SGVVVPGAGVCLNNAMHWFSTTPGHANVVAPGKTHEWPIAPLHLHRNGRFWGTIGTPG
                     SYGILVTTVQVLVHLLDFGLNLQDAIAAPRFRWIDDVGDPLPARAMRMESRVPEGTRG
                     ALATRGYEVELLGPWSMRVGGVQGILMDHSTGWLAGGADPRRNGYAIGW"
BASE COUNT          210 a          582 c          552 g          210 t
ORIGIN      
        1 cagccggacg ggcggcggag ggggggtcgg ccacaggatt tagtctagca cgccctacca
       61 gccgatcgcg tagccgttcc gccggggatc ggctcctccg gcgagccacc cggtggaatg
      121 gtccatcagg atgccctgca ccccgccgac ccgcatggac cacggcccga gcagctcgac
      181 ctcgtagccc cgcgtggcca gcgcgccccg ggttccttcg ggaacgcggg actccatccg
      241 catcgcccgg gcgggcagcg ggtctccgac gtcgtcgatc caccggaacc gcggcgccgc
      301 gatcgcgtcc tgcaggttga gcccgaagtc gagcaggtgc accagcacct ggacggtggt
      361 gaccaggatc ccgtaggacc cgggcgttcc gatcgtcccc cagaagcggc cgttccggtg
      421 aaggtggagg ggcgcgatcg gccactcgtg cgtcttgccg ggcgcgacga cgttcgcgtg
      481 cccgggcgtc gtcgagaacc agtgcatcgc gttgttcagg cacacgccgg ctcccgggac
      541 gaccaccccg ctcccgaacc cgtgccccag gctgtgcgtg atgttgacgg ccagcccgct
      601 cgcgtctgcg gcggcgaggt gggtggtcga cgccccgctg ccgtccgccc cgttcgcctc
      661 cgctcccgtt gcgccggcca cggccgaagg cgcgcgcagg cgccactccg cctgcgcgcg
      721 gagctcccgc gtgtgttcgg cggagagcag gcgagccacc ggtacctcga cgaacgccgg
      781 atcccccacg taccggtcgg tgtccagccg ggccgcccgc gccgcctcgg cgacggccgc
      841 gagatgatca gggctcaggt ggccggtgtt gcccacgtcg aacgtctcca gcacgttgag
      901 cgtctcgagc acctgaatcg cgctggtcgg cggcggcggc gcatacaccg tgaccccccg
      961 gtaggtcgtc tggatcgggg cggtccaccg cagcgtctcc ggatacccgc gaagatccgc
     1021 cgccgtcagc agccccccgc cgtcctgcat gaggcgaccg atcgcctggc ccagcgggcc
     1081 gccgtacagg tacccgattc cttccgcggc gatctgccgg aacgtcctgg ccaggtcggg
     1141 ctgaaccagg agtgccccga tcgccggcac gtggccgttg tggaagtagt tggcgacgcc
     1201 gtcccgcgtc aagcgcccgc cgctttctgt aaacatctgg tggtcgaacg gcgtgagggg
     1261 aatgccgcgc tcggccagct cgatcgccgg ctccaggacg tgcgcgaggg agagtttgcc
     1321 atactcggcc agcacccgcg cccaaccggc gaggttcccg ggaacggcgg gcgccaggta
     1381 cccggcgtcg gggggcccgg ccgggaaccg gtccggcgtc gccgcgccgg gtgcccggcc
     1441 cagaaagatc aggacccggg gagcctcccc cggcggggtg agcaccaggg tgccgcaccc
     1501 tgcgagtccc gacatgtacg gttccgcgac cccgatggcg gccgcggccg cccc
//
LOCUS       DASQGH010000089         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37244, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000089 DASQGH010000000
VERSION     DASQGH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37244"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VEW91_01695"
     CDS             <1..449
                     /locus_tag="VEW91_01695"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HYM90328.1"
                     /translation="DKVVKVGPDTLILTRRAATLGTIKPGDALGVAAKRGADGSLTAI
                     SINIFSPELWGRARKGQFPMASGDVMTNAVVTQYVDRVEGRMLYMEYGDVTAVISVPA
                     TTEIHRLTTIGLGDLKPGMRVTVRGLVNPDGAITASTIIVDQPGQG"
     gene            770..1795
                     /locus_tag="VEW91_01700"
     CDS             770..1795
                     /locus_tag="VEW91_01700"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90329.1"
                     /translation="MRTLTLCVSRLLFGLLIASVACGVVVHPEVRAAPGALTRVNVQL
                     GWIKNSEFAGLFVADQKGYYAAEGLDVNFISGGAGIDPMNIVRANPTYIGVVSSSGIL
                     INAVTKGAPFVAVAAFYQKHPNGFLLLADNPIKTYKDFEGRKIGVQPEGEYYLDVLSA
                     IYGVDKSKMEVVRVGFDPTPLLTGQIDAYMAWVVNQPYAVEKVGKKWKFMLLADTPGL
                     QFYAMLPFVTKDFLSKSPGVIERWLRASLRGWAYTVDHPDEVAGLVVQNYLSGGNVDA
                     ERWLLTQANPITVSADTKQHGLGWMDRTRWEAGIQTLLTYKQIERAPSVDEVMTDQFV
                     EKIAIKH"
     gene            1856..2635
                     /locus_tag="VEW91_01705"
     CDS             1856..2635
                     /locus_tag="VEW91_01705"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90330.1"
                     /translation="MIEIDRATMVFSAAEGPVTALRDVSFSVAEGEFVSLIGPSGCGK
                     STLLRLIADIYSPTSGIVRVAGQSPETSRRANLVGLMFQEPVLLPWLTVLQNVELPLR
                     FARRPTPLSARYLLEFVGLRGREKDYPHQLSGGMQQRVALARALVTDPKLLLMDEPFA
                     ALDELTRDRMGQWLLSIWEQARKTVLFVTHSIPEAVYLADRVIVMDAHPGRVIAIVDV
                     TLPRPRLEGVKESLDFFGLLGKLRALLRGDLPTTTPAVRSL"
     gene            2640..3416
                     /locus_tag="VEW91_01710"
     CDS             2640..3416
                     /locus_tag="VEW91_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90331.1"
                     /translation="MGRRPGRSLGRLISATVIITFLGAWELGERVTGYPPSFVAPLPS
                     AVVLFVIQHASLLAHHMTVTLVEAGAGFALGNLLAVGLAVASVYNETIERATMPFALA
                     LRSIPLIAIAPVLVFLLGLGWQSKVAVAVLVSFFPTLVNMIVGLRSVDYRLLELMHVL
                     NAGWWQVLVQVRFPSSWPAFFAALKIAVPSSILGAAVAEWINASAGIGYLIIVSTYQF
                     NTVQLYSTMFVTTAVSAGVYLAVVWLETRLLPWRKSLSSS"
     gene            3517..4353
                     /locus_tag="VEW91_01715"
     CDS             3517..4353
                     /locus_tag="VEW91_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine phosphorylase"
                     /protein_id="HYM90332.1"
                     /translation="MKFPDDLREPGVRVAERGLSFATPWGRTDNWQVIEYDRSITVDG
                     QPRLALNVFAHGWPVDTIDHSVHRKVFWVLGQAGVKKVLSDSTCGSLNRALQPRDFII
                     TSDILDLGQTRYSTLPGRFAYVCRGAQMFCPSLAHALEDVARELWPAGARVYGFANRM
                     IMGHTWGPRFETPTEARALALLGSDAVNQSIAPEATNAREIGACFISGSYVVNYVDGI
                     IPEEWGALDTIHDELGEVATRISLRTVARAELTEACGCGTYRKARPSKYRVGAQSSPG
                     GR"
     gene            complement(4362..>5372)
                     /gene="add"
                     /locus_tag="VEW91_01720"
     CDS             complement(4362..>5372)
                     /gene="add"
                     /locus_tag="VEW91_01720"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HYM90333.1"
                     /translation="TTIIELAREYGFPLPAYEPDALRRYVQVMSDTPADLGHILRTVA
                     AFLRRCFPSRRAIARVTVEMIEDAWRDGIRYLEVRFSPWYLGAEHGLALGNVLDGVAD
                     GLTAARARYPVRTAIILGMTRESGLDACAQTARLALASANGMVTGVDLSGDEEAYPAG
                     QYSRIFEQIRADGRLGITVHAGEAAGPESVRDAIEILGAHRIGHGVRVVGDPAAVDLV
                     RSRGITLETCPTSNVLTGAVAGFEAHPLRTLLAGGVAATINTDDPSWFDTTLTDEYCL
                     ALTRLGLTFDGLRQAVLHAARGAFLPVEERERLARDLATAYDGARPDFAALNLTGGGA
                     GS"
BASE COUNT          927 a         1704 c         1782 g          959 t
ORIGIN      
        1 ccgacaaggt cgtcaaggtc ggaccagaca cgctcattct cacccggcgg gccgcgacgc
       61 tggggacgat caagccgggc gatgcgctgg gggtcgccgc aaagcgaggg gcggacggat
      121 cgctgacggc gatctcgatc aacatctttt cccccgaact ctggggtcgg gcccgcaagg
      181 gccagtttcc gatggcgtcc ggagacgtca tgacgaacgc ggtggtcacg cagtatgtcg
      241 accgcgtcga ggggcgcatg ctctatatgg agtacgggga cgtcaccgcg gtcatcagtg
      301 tcccggccac gacggagatc caccgtttaa ccacgattgg gctcggtgac ctgaagccgg
      361 ggatgcgggt caccgtccgg gggctcgtta atccggatgg ggcgatcacg gcatccacca
      421 tcatcgtcga tcagcccggc caagggtaga gtgtctgctg cgtaagtatc ttggaggtct
      481 cccacggaag gtgttcgagt cccggcaccc tctcggcagc gagttacgct ttactcgttc
      541 agcgagacgc agcttcaaag ggaatcgtcc tccttggaac tagcaccctc atcgtttcga
      601 gaatgaagta gagatcgtcc tcccggtgtc cgacgcatgg ctgaccactc ctgggcagtg
      661 ccgcatcggc agggcgcatt gcctggcgtg cggaccggga ggagtcggct gtcggatcca
      721 gaacacccgt gtacggacgg gatcgggatg tcgcctgcgg ggggccgcca tgagaacgct
      781 gacgctgtgc gtatccagac tgctcttcgg cctgctgatc gcaagcgtgg cgtgcggggt
      841 cgtcgttcac cccgaggtac gggcggcgcc gggggcgctc accagggtca atgtgcagct
      901 cggctggatc aagaactcgg agtttgcagg cctgtttgtc gcagaccaga agggctacta
      961 cgctgcggaa gggctggacg taaacttcat ctcaggcggg gctggcatcg acccgatgaa
     1021 catcgtccgc gccaacccca cctatatcgg ggtcgtgtcc tcgagcggca tactgatcaa
     1081 cgccgtgacg aagggcgcgc cgttcgtcgc cgtcgccgcg ttctaccaaa agcaccccaa
     1141 cgggttcctt ctcctagcgg acaacccgat caaaacctac aaagacttcg aagggcggaa
     1201 gatcggcgtc cagccggaag gtgagtacta tttggacgtt ctgtcggcga tttacggcgt
     1261 tgacaagtcg aagatggagg tcgtccgagt tggctttgat cccacgcccc tcctcaccgg
     1321 ccagatcgat gcgtacatgg cctgggtcgt gaaccagccg tacgccgttg agaaggtcgg
     1381 caagaaatgg aagtttatgt tgctcgccga cacacccggc ctgcagttct acgcgatgct
     1441 gcccttcgtg accaaggact tcctcagcaa gagtcctggc gtgatcgagc gatggctgcg
     1501 cgccagcctg cggggctggg cgtacaccgt cgaccatccg gacgaagtgg ccggcctggt
     1561 cgtgcagaac tatctgtctg gcggaaatgt ggatgcggaa cggtggctgc tcacacaggc
     1621 gaatcccatt accgtgtcgg cggacacgaa gcagcatggc ctcggatgga tggaccggac
     1681 gcgttgggaa gctgggatcc agaccctgct gacctataag cagatcgagc gagcgcccag
     1741 cgtcgacgag gtgatgaccg accagttcgt cgagaaaatt gcgatcaagc actgacagcc
     1801 ggatccgccc ccatacgaca ggcatcgcct gagcgggcat ccggccgctg agtccatgat
     1861 cgagatcgat cgcgccacga tggtcttttc tgcggctgag ggaccggtga cggccctgcg
     1921 cgacgtgtcc ttcagcgtcg ccgagggaga attcgtctcg ctaatcgggc cgtcgggatg
     1981 cggaaaatca acgctcctgc gcctcatcgc cgacatctat agcccgacat cgggcatcgt
     2041 tcgggtggcc ggacagagcc ccgaaacgtc gcgccgggcc aatctcgtcg gcctgatgtt
     2101 tcaggaaccc gtgctcttgc cgtggctcac tgtcttacag aatgtcgagc tgcctctccg
     2161 gttcgcacgg cgcccaaccc cgctcagcgc caggtacttg ctcgagttcg tgggtctccg
     2221 ggggagagaa aaggattacc cgcatcaact ctccggaggc atgcagcagc gcgtggccct
     2281 ggcccgggcg ctcgtaaccg atccgaaact cctgttgatg gatgagccgt ttgcggcctt
     2341 ggatgagttg acgcgtgatc gaatgggaca gtggctgctc tcgatctggg agcaggcacg
     2401 gaagactgtc ctcttcgtga cccactccat tccggaagcg gtgtacctcg ccgaccgcgt
     2461 catcgtgatg gatgcgcatc cgggccgggt catagccatc gtcgacgtaa cgttaccacg
     2521 cccccgcctc gagggcgtca aggaaagtct cgacttcttc gggctcctgg ggaagctccg
     2581 tgcgctgctg cgaggagacc tccccaccac aacaccggcg gtgaggagcc tctagccgga
     2641 tggggcgccg tccgggtcgc agcctgggcc ggctgatctc cgccacggtc atcatcacct
     2701 ttctgggtgc gtgggagctg ggggaacggg tgacgggcta tccgccgagc ttcgtcgccc
     2761 cactgccgag cgcggtcgtg ctcttcgtca tccagcatgc gagcctcctg gcgcaccaca
     2821 tgacagtgac cctggtcgag gcgggtgccg ggttcgcgct gggcaacctc ctggcggtcg
     2881 gcctggccgt cgcgtctgtc tacaatgaga ccatcgagcg cgccacgatg ccctttgcgc
     2941 tggcccttcg cagcatcccc ctgatcgcga tcgcgccggt gttggtcttc ctgctgggac
     3001 tgggctggca gtcgaaggtg gcggtggccg tgctggtctc gttcttccca acactggtca
     3061 acatgatcgt cggcctgcga tccgtcgatt accggctgct cgagctgatg cacgtgctga
     3121 atgcggggtg gtggcaggtc ttggtgcagg tccggttccc atcgtcgtgg cccgcgttct
     3181 tcgccgcgct caagattgcg gtgccgtcct caattctggg ggccgccgtg gccgagtgga
     3241 tcaacgcgag cgccgggatc ggctatttga tcatcgtgag cacctatcag ttcaacaccg
     3301 tccagctgta ttcgacgatg ttcgtgacga cggcggtatc agccggagtc tacctggcgg
     3361 tcgtctggct ggaaacccgt ctgcttccct ggcggaagtc tctctccagc agctagcgcg
     3421 ggctcgagtc gtacaggcag cgagggggtg acacgagtgg atcgcgacat accaaagaca
     3481 ccctttgctt tgatctccgg cagcgctggc tgggggttga agtttccaga cgacctccgg
     3541 gaaccgggcg tacgcgtcgc cgagcgtggc ctgagcttcg cgacgccctg ggggcgcacg
     3601 gacaactggc aggtgatcga atacgacagg agcatcaccg tggacgggca gccccgtctc
     3661 gcgctcaacg tgtttgccca cggctggccg gtcgatacca tcgatcactc ggtccaccgc
     3721 aaggtgttct gggtgctggg ccaggcaggt gtgaagaagg tactctcgga cagcacctgc
     3781 gggtcgctca atcgcgcact ccagccccgg gacttcatca tcacgagcga catcctggac
     3841 ttgggccaga cccggtactc cacgctgccg ggaaggttcg cttacgtgtg ccgcggagcg
     3901 cagatgttct gccccagcct cgcgcatgcc ttggaggacg tggcgcgcga gctgtggccg
     3961 gcaggcgcac gcgtgtacgg gttcgcgaac cgcatgatca tgggccacac atgggggccg
     4021 cgcttcgaga ccccgacaga agcccgggcc ttggcgctgc tcggctccga cgcggtcaac
     4081 cagagcatcg cgccggaggc cacgaacgcc cgcgagatcg gggcatgctt catcagcggt
     4141 tcgtacgtgg tcaactacgt ggacgggatc attcctgagg aatggggtgc cctggacacg
     4201 attcacgacg agctcggcga ggtggccacc cgcattagcc tccgtaccgt cgcgcgcgcc
     4261 gaactgaccg aggcatgcgg ctgcggtacc taccggaagg cgcgcccgag caagtaccgg
     4321 gtcggtgccc agtccagccc agggggcaga taacccctgg gctaagagcc ggcaccgcca
     4381 ccggtgagat tcagggccgc aaagtcggga cgcgccccat cgtaggcggt ggccaggtca
     4441 cgcgccagtc gctcccgttc ctccaccggc aggaacgcgc ctcgcgccgc gtggagcacg
     4501 gcctggcgca gtccgtcaaa tgtgaggccc agtcgggtaa gcgccaggca gtactcgtcg
     4561 gtcagcgtgg tgtcgaacca gctggggtcg tccgtgttga tggtcgcggc gacgcccccc
     4621 gcgagcagcg tgcgaagcgg atgggcctcg aagccggcaa ccgccccagt gagcacgttg
     4681 ctggtggggc acgtctccag cgtgatgcct cgcgaccgca cgagatcgac ggcggcggga
     4741 tcgcccacca ctcgtacccc gtgcccgatc cggtgcgcgc ccaggatctc gatggcgtcg
     4801 cggacgctct cagggccggc ggcctccccc gcgtgcacgg tgatgccgag gcgcccatca
     4861 gcccgtatct gttcgaagat acggctgtac tgtccggcgg gataggcttc ttcgtcgcct
     4921 gacaggtcaa cgccggtgac catgccgttc gccgacgcga gcgcgagtcg ggcggtctgc
     4981 gcgcaggcgt cgaggcccga ctcgcgggtc atgccgagga tgatcgcggt gcgaaccggg
     5041 tagcgcgcgc gcgcggcagt caacccgtcc gccaccccgt ccaggacgtt gccgagtgcg
     5101 agcccgtgct ccgccccgag gtaccagggc gagaaccgga cctccaggta acggatgcca
     5161 tcgcgccagg cgtcttcgat catctcgacc gtgacgcgcg cgatcgctcg ccgggaagga
     5221 aagcagcgcc gcaggaacgc tgcaaccgta cgcaggatgt ggccgaggtc ggccggcgta
     5281 tcggacataa cctggacgta ccgccgcaat gcgtccggct cgtacgcagg cagcggaaac
     5341 ccgtattctc gtgccaactc gatgattgtc gt
//
LOCUS       DASQGH010000090         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17385, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000090 DASQGH010000000
VERSION     DASQGH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17385"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VEW91_01725"
     CDS             <1..1050
                     /locus_tag="VEW91_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90334.1"
                     /translation="NVRKQYGHAAALRGISVTVKQGELLAIVGPSGCGKTTTLQILAG
                     FLIPDGGEVWADDRLLSSPGGAVPPERRRMSLVFQSYALWPHMTVFHNVAFGLETRRL
                     GRDEITRRVQAILEVVNLRGYDARYPHELSGGQQQRVALARALVVQPETLLLDEPLSN
                     LDASLREQMRFEIRRIHRETHITMVYVTHDQTEAMVIADRIAVMNAGEIEQIGTPQEI
                     YELPQTHFVASFIGQTNCLEARLIEPGVVRCGEVRVRTAGISPFQPGNAVVLCIRPHA
                     VRVHRGTQADPDGQNRVRGRVLEQAYLGDLQDLRIALPGDLEIRAFVPSSQSYRAGEE
                     IVVELPLDACRLVRP"
     gene            complement(1047..1997)
                     /gene="speB"
                     /locus_tag="VEW91_01730"
     CDS             complement(1047..1997)
                     /gene="speB"
                     /locus_tag="VEW91_01730"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYM90335.1"
                     /translation="MARARFEPPDAFESPRFAQLSTFMRLPHTRDARGLDVAILGIPF
                     DGGVSFRPGARFGPKEIRNHSLLIRPYHPVLRTLPFSRLRVADCGDVDPNPMDIVDTY
                     GRIEAGVNEVLAAGAIPACVGGDHSISLPILRAVARRHGAVALVHFDSHQDMWEQYFG
                     NRYFHGTPFRRALEEGLYLPRATVQFGIRGSVYAEGDFAFGEAHGVTVVRAEEIHARG
                     VEPALARLDRVRGHKVYVSFDIDVVDPAFAPGTGTPEVGGLSSAQVLALLRGLAGLEI
                     VAFDIVEVSPPYDQSGITAMLAANVLFELMSVLALGAGSR"
     gene            complement(1978..>2214)
                     /locus_tag="VEW91_01735"
     CDS             complement(1978..>2214)
                     /locus_tag="VEW91_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90336.1"
                     /translation="VTLDIEEALFLGDRVYVMTARPGRFRAEVSLTLPRPRTLEVTTS
                     DEFVSAKRRVLAMIREETARTLGGTVEADGARAV"
BASE COUNT          347 a          794 c          770 g          303 t
ORIGIN      
        1 aacgtcagga agcagtacgg ccacgccgcg gccctccgcg ggatcagcgt cacggtcaag
       61 cagggcgagc tgctcgcgat cgtcggcccc tcggggtgcg gaaagacgac cacgctgcag
      121 atcctggcgg ggtttctcat cccggacggc ggcgaggtct gggccgacga tcggttgctc
      181 tcgagccccg gcggggcggt cccccccgag cggcgccgga tgtcgctggt cttccagagc
      241 tacgcgctct ggccgcacat gacggtcttc cacaacgtcg cgttcggcct ggagacgcgc
      301 cggctgggcc gcgatgagat cacccgccgg gtgcaggcga tcctcgaggt cgtcaatctc
      361 cggggctatg atgcgcgcta cccgcacgag ttgtcgggag gccagcagca gcgcgtcgcg
      421 ctggcgcgcg ccctggtcgt gcagccggag accctgctcc tggacgagcc cctctccaac
      481 ctcgatgcca gcctgcgcga gcagatgcgg ttcgagatcc gccggatcca ccgcgaaacg
      541 cacatcacga tggtctatgt cacccacgac cagaccgaag ccatggtcat cgcggaccgc
      601 atcgcggtca tgaacgcggg cgagatcgag cagatcggca cgccccagga gatctacgag
      661 ctcccccaga cgcacttcgt cgcctcgttc atcggccaga ccaactgcct tgaggcgcgc
      721 ctgatcgaac cgggggtcgt ccgctgcggc gaggtgcggg tgcgcaccgc cgggatctcg
      781 ccgtttcaac cggggaacgc cgtcgtcctc tgcatccgac cgcacgcggt ccgggtgcat
      841 cgcgggaccc aggccgatcc cgacggccaa aaccgggtgc gcggccgggt gctcgagcag
      901 gcctacctgg gagacctgca ggacctccgc atcgccctgc cgggggatct cgagatccgg
      961 gcgttcgtgc cgagcagcca gagctaccgg gccggcgagg agatcgtggt ggaactgccg
     1021 ctggacgcct gccggctggt ccggccctaa cggctgccag cgcccagcgc cagcacgctc
     1081 attaactcga agaggacgtt ggccgcgagc atcgcggtga tcccgctctg gtcgtacggc
     1141 ggggagacct cgacgatgtc gaacgccacg atctccagcc cggcgaggcc gcggaggagg
     1201 gcgaggacct gggcgctcga gaggccgccc acctcgggcg tgccggtgcc gggcgcgaac
     1261 gcgggatcca ccacgtcgat atcgaacgac acgtagacct tgtgcccgcg gacgcggtcc
     1321 aggcgggcca gcgcgggctc gacgccgcgg gcgtggatct cctccgcgcg gaccaccgtg
     1381 acgccgtggg cctccccgaa tgcgaagtcg ccctccgcgt acaccgaccc gcggatcccg
     1441 aactgcacgg tcgcccgggg aaggtagagg ccctcctcga gcgcccgccg gaacggggtg
     1501 ccgtggaagt aacggttgcc gaagtactgc tcccacatgt cctggtggct gtcgaagtgc
     1561 accagcgcga ccgcgccgtg ccggcgcgcg accgcccgca ggatcgggag cgagatcgag
     1621 tggtcgcctc cgacgcacgc cgggatggcc ccggcggcga ggacctcgtt caccccggcc
     1681 tcgatccggc cgtaggtatc gacgatatcc atcgggttcg ggtccacgtc cccgcagtcg
     1741 gcgacccgga gccgcgagaa cggcagggtg cgcagcaccg ggtggtacgg ccggatcaag
     1801 agcgagtggt tgcggatctc cttgggcccg aaccgggcgc ccggccggaa gctcaccccc
     1861 ccgtcgaacg ggatcccgag gatcgccacg tccaggccgc gggcgtcccg ggtatgcggc
     1921 agccgcatga acgtcgagag ctgggcgaac cggggagact cgaaggcgtc cggaggctca
     1981 aaccgcgcgc gcgccatcgg cctccaccgt tccccccagc gtcctggcgg tctcctcgcg
     2041 gatcatcgcc agcacccggc gcttcgcgct cacaaactcg tcactggtcg tcacctcgag
     2101 ggtccgcggc cgtgggagcg tcagcgacac ctcggccctg aaccgcccgg gccgcgcggt
     2161 catcacgtac acccggtccc cgaggaagag cgcttcttcg atgtcgaggg tgac
//
LOCUS       DASQGH010000091         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28751, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000091 DASQGH010000000
VERSION     DASQGH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28751"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VEW91_01740"
     CDS             complement(<1..605)
                     /locus_tag="VEW91_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYM90337.1"
                     /translation="MAVPIGAHVSISGKIYDAVSRAQALGCECLQIFFGSPRQWRLIS
                     YPPEDLEEFRRRRARVRIDPLVAHTPYLINLASPDPTVYRRSVVSLAHSLQGMDALGG
                     LAAITHIGSALGTPWPEARARVVWALRAALRESTRAMILLEGSAGGSLGGTFEQLGEI
                     LDEAGEGRRLGVCLDTAHLFAAGWDLRAPAGVAAMVKACPRA"
     gene            708..>1616
                     /locus_tag="VEW91_01745"
     CDS             708..>1616
                     /locus_tag="VEW91_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HYM90338.1"
                     /translation="MGEQTRWGFSTQAIHGGGIPDANKSVVTPIYQTATFRYDTVEEG
                     ARLGAETGPGYFYTRWGNPTTNAFEQKMALLEGGEAALATSSGMAAISTAVITLLRAG
                     DHVVAPKAVYQATFGLLHSILPAYGVEATLLDDPDLGAYERALRPSTRLLYIETPNNP
                     VLGIIDIAGVVALARSHGARTIADNTFATPYNQTPLALGVDLVCHSATKYLGGHHDVT
                     AGVIVGSRELIRRCVLTMRTFGGVLDPFAAYLLIRGLATLGLRVERHNASALALAQHL
                     AGHPKVARVHYPGLPAHPRHAVAARQM"
BASE COUNT          241 a          566 c          589 g          220 t
ORIGIN      
        1 gcgcgggggc aggccttgac catggcggcg actcctgcgg gggcgcgcag gtcccacccc
       61 gcggcaaaca ggtgggcggt atccaggcag acgccgaggc ggcggccctc gcccgcctca
      121 tctaggatct cccccagttg ctcgaaggtg cctcccaggc ttccgcccgc gctgccctcg
      181 agcaggatca tcgcgcgcgt gctttcccgc agtgccgcgc ggagcgccca caccacccgg
      241 gcgcgcgcct ccggccaggg ggtcccgagc gcgctgccga tgtgcgtgat cgctgccagg
      301 ccgccgaggg catccatgcc ctgcagggag tgcgcgagcg agacgaccga ccgccggtac
      361 acggtggggt cgggggaggc gaggttgatg aggtacggcg tgtgggcgac cagcgggtcg
      421 atgcggaccc gcgctcgcct ccggcgaaac tcctcgaggt cctccggggg gtaggagatg
      481 agccgccact ggcggggact cccaaagaag atctgcaggc attcgcaccc cagcgcctgc
      541 gcacgggaga ccgcgtcgta gatcttgccg ctgatcgaca catgggcgcc gatgggcacg
      601 gccactggtt cggggtcgcg cgtggggacc tcctgcggcg cgggcggagc aggagcgcct
      661 ccaccatggg cgaactgccc cggcagagcg aagtggggag cgcgcgaatg ggtgagcaga
      721 cgcggtgggg attcagcacc caggcgatcc acggaggcgg gatccctgat gccaacaagt
      781 ccgtggtcac gccgatctac cagacggcga ccttccggta cgacaccgtc gaggaggggg
      841 cgcggttggg cgcggagacc gggcccgggt acttttacac gcgctggggg aatccgacga
      901 cgaacgcgtt cgaacagaag atggccctgc tcgaaggggg ggaggcggcg ctggccacct
      961 catccgggat ggcggccatc tccacggcgg tcatcaccct gctgcgggcc ggggatcacg
     1021 tggtcgcccc caaggccgtc taccaggcga cgttcgggct gttgcactcg atcctgcccg
     1081 cgtacggcgt ggaggcgacg ctcctcgacg acccggacct cggcgcgtac gagcgcgcgc
     1141 tgcggcccag cacccgcctg ttgtacatcg agacgccgaa caacccggtg ctcggcatca
     1201 tcgacatcgc cggcgtggtg gcgctggccc gctcccacgg cgcccggacg atcgccgaca
     1261 acacgtttgc gacgccgtac aatcagacgc cgctggcgct gggcgtcgac ctcgtctgcc
     1321 acagcgccac caagtacctg ggtgggcacc acgatgtcac cgcgggcgtg atcgtggggt
     1381 cgcgggagtt gatccgccgg tgcgtcctca cgatgcggac ctttgggggc gtgctggacc
     1441 cgttcgccgc ctacctgctg atccgggggc tggcgaccct cgggctgcgg gtggagcggc
     1501 acaacgccag cgccctcgcg ctcgcgcagc acctcgccgg ccatcccaag gtagcgcgcg
     1561 tgcactaccc gggattgccc gcgcatcccc gccacgcggt cgccgcccgg cagatg
//
LOCUS       DASQGH010000092         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_368, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000092 DASQGH010000000
VERSION     DASQGH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="tdh"
                     /locus_tag="VEW91_01750"
     CDS             <1..1020
                     /gene="tdh"
                     /locus_tag="VEW91_01750"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HYM90339.1"
                     /translation="AGPGLEIRTVPVPAPGPGEVLVRVRAGGICGTDLHIEGWDPWAA
                     SRLRPPRVIGHEFCGTVVETGPEVHVAAPGDFISGESHVSCGRCHLCRQGQAHICEAL
                     QIIGVDRDGAFAEYVVLPAQNAWKMDPTTPLEVAAVMDPFGNAVHTALATDLGARSVI
                     VMGCGPIGLCAILVARKAGAVPVVAVDVNPYRLRLAGRLGADRVLDGRTEDIPRIIRG
                     LTDGAGADVLLEFSGSAQGLHTGLAGLRNGGFASLLGLPGRPVELDLANEVIFKGLRL
                     QGIFGRRLWETWYEAAALLASGVDIRPVITHRLPMERFAEAFALMRSGECGKVILTVE
                     GVTDA"
     gene            1013..>1675
                     /locus_tag="VEW91_01755"
     CDS             1013..>1675
                     /locus_tag="VEW91_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM90340.1"
                     /translation="MRDPLAFLDDEIASLRERGVFRWPRELQGEQAPVCTYDGRRVVN
                     LCSNNYLGLAAHPRLKDAARRAIAEYGVGSGAVRTIAGNMTIHQTLERRLAAFKRTEA
                     ALLFQSGFTANAGTVAAILGREDVVVSDELNHASIIDGCRLSGAERKIFPHKDVAAAR
                     RCLEESRKARRVLVITDGVFSMDGDLAPLRDLASLAREFGAIMMVDDAHASGVVGAGG
                     RVT"
BASE COUNT          221 a          566 c          632 g          256 t
ORIGIN      
        1 gccggtccgg gcctcgagat ccgcacggtc ccggtccccg cgcccggtcc cggtgaagtg
       61 ctcgttcggg tccgcgcggg cggcatctgc gggaccgatc tccatatcga gggctgggat
      121 ccgtgggcgg cctcccgcct ccgcccgccg cgggtgatcg ggcacgagtt ctgcggcacc
      181 gtggtcgaga ccggcccgga ggtgcacgtc gccgcacccg gcgatttcat ctccggcgag
      241 tcccacgtca gttgcggccg ctgtcacctg tgccggcagg ggcaggcgca catctgcgaa
      301 gcgctccaga tcatcggggt cgaccgggat ggggcgtttg ccgagtacgt ggtcctgccc
      361 gcgcagaacg cctggaagat ggaccccacg accccgctcg aggtcgcggc ggtgatggac
      421 ccgttcggga acgccgtgca caccgccctc gccaccgatc tgggagcccg ctcggtcatc
      481 gtgatggggt gcggcccgat cgggttgtgc gccatcctgg tggcgcgcaa ggccggcgcc
      541 gtgccggtgg tggccgtcga cgtgaacccg tatcgcctcc ggctcgccgg gcgcctcggc
      601 gccgaccggg tgctggatgg ccggaccgag gacatccccc ggatcatccg cggcctcacc
      661 gatggggcgg gggccgacgt cctgctcgaa ttctcgggga gcgcgcaggg gctgcacacc
      721 gggttggcgg ggctccggaa cggcgggttc gcatcgctcc tcggcctgcc cggccggccg
      781 gtggaactgg acctggccaa cgaggtgatc ttcaagggcc tccgcctgca ggggatcttc
      841 ggccggcgcc tgtgggagac gtggtacgaa gccgccgccc tgctcgcgtc cggcgtggac
      901 atccgccccg tgatcaccca ccggctgccc atggaacggt tcgcagaggc gtttgccctg
      961 atgcggtcgg gcgagtgcgg gaaggtgatt ctgaccgtgg agggcgtcac ggatgcgtga
     1021 tcccctggcg ttcctggacg acgagatcgc ctccctccgc gagcgcggcg tgttccgctg
     1081 gccgcgggaa ttgcagggcg agcaggcccc ggtctgcacg tacgacggcc ggcgggtcgt
     1141 caacctctgc tcgaacaact acctcgggct cgcggcgcac ccgcggctca aggacgccgc
     1201 gcgccgcgcg atcgccgaat acggggtggg gtcgggcgcg gtccggacga tcgcgggcaa
     1261 catgacgatc caccagacgc tcgagcggcg gctggcggcg ttcaagcgca ccgaggcggc
     1321 gctgctcttt cagtccgggt tcaccgcgaa cgcggggacg gtggcggcga tcctcggtcg
     1381 cgaggacgtc gtcgtcagcg acgagctgaa ccacgccagc atcatcgacg gctgccggct
     1441 gagcggcgcg gagcggaaga tctttcccca caaggatgtc gcggcggccc gccggtgcct
     1501 cgaggaatcg aggaaggcga ggcgtgtgct cgtgatcacc gacggcgtgt tcagcatgga
     1561 cggcgatctg gccccgcttc gggacctggc gtcgctcgcc cgcgagtttg gcgcgatcat
     1621 gatggtggat gatgcccacg ccagcggggt ggtgggggca gggggacggg ttacc
//
LOCUS       DASQGH010000093        12621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51314, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000093 DASQGH010000000
VERSION     DASQGH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51314"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VEW91_01760"
     CDS             <1..381
                     /locus_tag="VEW91_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90341.1"
                     /translation="TCSGCNGIPSLTLQPLGGSQQPLVKGVDSMSIQYVLNRLYSTAP
                     GECNAQTGGTSSLCVVNLPTQAPSIAGDWQVVRAIIFALDARSITKVRASGSADGYFH
                     LSDTVEISPRNLIYQQTRVPWTPY"
     gene            523..1332
                     /locus_tag="VEW91_01765"
     CDS             523..1332
                     /locus_tag="VEW91_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HYM90342.1"
                     /translation="MSSASDLRTVTGATPAEIRRRIRGGEWREPTAGLAPGFAQANLV
                     VVPREIAYDFLVFCQRNPKPCPLIDVTDPGSPEPSHAAPGADLRTDLPRYRIYRRGEL
                     DAEVTEITRYWRPDLVAFLLGCSFTFETAMQRAGLPVRHIADRRNVAMYITSIPCRPA
                     GGLHGPTVVSMRPVPREHLARAILVTGRYPRAHGAPVHVGDPAKIGIRDLARPEFGDP
                     PRIEPDDVPVFWACGVTPQAVAQAARVELMITHAPGHMFITDLLDEDLAAG"
     gene            1363..2652
                     /locus_tag="VEW91_01770"
     CDS             1363..2652
                     /locus_tag="VEW91_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90343.1"
                     /translation="MRRSWLTRREMLKLAGGAVVFAGGGWRAGPAAAQAEEVKIGCLY
                     PLTGSLARLGADMRKAVDLAAEIVNTPHPELAPLTLAATSGLPRLGGRKIGLVWADAK
                     DPATARAEAERLIEQERVAALMGCYASAFTNTASLAAETRGIPFLNPESSSPTLTQRG
                     FKWFFRTGPHDGTFTAMFYRMFADLKQRGVRIQKFAILSENTEFGATAARVEEEAGKG
                     AGYQMIAREMYTSPPASLDAELARIRAANPDVLIANGYLIDAVTTIKTLRTMRYFPPG
                     LVVQDSGYVVPDYLKETGKDGLYVISRLAWALGLGVRKPLVKKVNDLYRSRFNEDMDE
                     TNARSFTGLLALASAINQGGGVTSTQILQGLLQTNIPGSDLIMPWQGIKFDQDGQNVY
                     ALGVMGQRLGDGGFKVVWPFDLAETNIVWPAPAWDKR"
     gene            2735..3601
                     /locus_tag="VEW91_01775"
     CDS             2735..3601
                     /locus_tag="VEW91_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90344.1"
                     /translation="MDLLLPASVAGLVMGCIYALVSLGLGLIWGVMDVVNFAHAEYMM
                     LAMYATYYAWAWLHLSPFAALPAVIALGFGAGVATYLLLIRRITGAPMVTQIFATFGL
                     LFFLRYLAFAVFGPDFRGIAWEPANGSIEILGVPVSTAKAWAAGAALLTFAALHGFLQ
                     YTKVGKAVRATAQSPGAALALGIEVERIYMLAWGLSIATAAVAGVFLLPFYQVSPTVA
                     DAFLLLTFASVVLGGFGSLLGPLIGGVAIGLIEGIAGTLLSPTFKLVFVFAVFLLVLF
                     VRPRGLLGEWGA"
     gene            3598..5376
                     /locus_tag="VEW91_01780"
     CDS             3598..5376
                     /locus_tag="VEW91_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HYM90345.1"
                     /translation="MSGIARWAWIAVWGALVLLPQAIHNTYLLNGLILVFIYGAAAQA
                     WNLLGGYAGQLSFGHVVFFGLGAYTSTLLLIRLGLSPWFGMWVGAGVAVLVALAVGYP
                     TFRLRRHYFALATLALAEMARILFLNWPLVGAAVGLFLPVRLMNAPWTMMWQAKPPYY
                     YLALALFGAACGVVLLIDRARLGLYLRTIDLDEDAASALGIPTRRYKLLVIAMSAGLT
                     ALAGTLFAQYVLYIDPSSVLDPARSVLFAVMALLGGRGTVAGPALGAGFLVLLSQYAG
                     GTLGGLGRGYDYVIYGLAIMLVALYEPRGLVGLIHRLGGRRGAAPPASAGAPRAEGRE
                     PARPARAGAAPPLLEVAGLAKHFGGVEALRGVDLEVGRGEIVGVLGPNGAGKSTLFDC
                     VTGFLRPSGGRVLLRTAAGARALTGRPPHWIAQQGLARTFQLIRVFPSLTTWEHLLVG
                     QEHRGEPLLAAFSASPPEVREQARALMRLVGLQDARDTPAGALSYGQQKLLALATALM
                     RRPSLVLLDEPVAGVNPAVIERLKAYIRGENAGGATFLIIEHNMQVLMELAERLYFMA
                     DGRMVVEGPPQAIQENAQVLAIYYGR"
     gene            5366..6085
                     /locus_tag="VEW91_01785"
     CDS             5366..6085
                     /locus_tag="VEW91_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM90346.1"
                     /translation="MAGDPPLLEVADVHAGYGEQEILRGLSLVVRPGEVVCVIGPNGA
                     GKSTLVRTVFGFLRPLRGTIRFGGESIGGRRPPELLRRGITYVPQGVSAFLNLSVEDN
                     LYLAAYTRGDRDVRHDIEQLLQLFPALAAGRRRPARTLSGGERRMLEIARVLLLHPRL
                     VLLDEPTIGLAAATMAFVYEKIREIQRRGVAILLVEQNARTALKNSDRAYVLEQGRVR
                     FEGSGEEILAHPEVRRAYLGG"
     gene            6089..8149
                     /locus_tag="VEW91_01790"
     CDS             6089..8149
                     /locus_tag="VEW91_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013266552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYM90347.1"
                     /translation="MAHYRIGVDVGGTFTDLIAAEEGAGTLVPVKVLSTPRAPADGVL
                     AAFHQFLEGRPGRPTYLVHASTIGTNLFLGQVGLELPRAALLTTEGFRDVLEIGRQRR
                     AELYNLFYQRPRPLVDRRFRIGIPERIDATGRVVRPLDEGAVGTAAARLARERIEAVA
                     IAFLHGYANPSHERRAKAILQAALPEAVIVASHEVDPEYREYERTSTTVISALLSPVV
                     SRYLRGLSSRLRDEGHALPLYAMQSSGGITTIEGAAATPATLIESGPAAGVVGAAALG
                     RRLGYVRVLSFDMGGTTAKAGAIIGGRPEVVAEYEVGGTVHAGRIVRASGYPVRAPFI
                     DLAEVSAGGGTVAWVDGGALRCGPVSAGADPGPACYGRGGDRPTVTDAHLLLGWLSPR
                     GLLGGRMPLSAEAAARAMASVAQPLGLDQTATAAGIVRVVDAQMVRALRIVSLERGYD
                     PREMVAIAFGGAGPMHAAALADELGVREVVIPPNPGLFSAVGLLAADLRCDRVESVMR
                     PVEQVDPDSLETRFRRLEAAAQEGVSDGQGGREVVVRRALDLRYIGQSYELTVPGEVP
                     LGAAAEAFRVRHAEIYGYAPPREPVEVVNARVTALGIMPPLSLWEPPPGPRSPDRGEV
                     RRAYFEGRWVDVPVVARKGLGAGGAIPGPAIVEEDEATTIVPPGWSARIGEAGTLHLR
                     RGAA"
     gene            8146..9774
                     /locus_tag="VEW91_01795"
     CDS             8146..9774
                     /locus_tag="VEW91_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYM90348.1"
                     /translation="MIPDPVTVEVIRNAMIYAAEEMGIALRNASYSPNIKERMDHSCA
                     LFDAAGRLIAQAEHIPADLGSLPWGVRATLALLQHRPPRPGDLFILNDPYIAGTHLND
                     IMLVRPLFWQGTIVGYSVNKAHHVDVGGRTPGSLSSDARELCEEGIVIPLLPILREGV
                     EVPETVEVILANVRSPDTTRGDLRAQIAAASLGERRVAELLDRYGREAVEAAWEAILD
                     QGEASARRAFAVLPPGRYTAEDCLEDDLADGGLRWIRVAVIVAPGAITVDFAGTDPQV
                     PRPINAILGVTNAAVLYALKAVCDPDSVMNDGWLRPVTLRVPLGTVINPVRPAPVGVG
                     NTETNQRIADTVLRALAKAAPGRVPAASNGSMTNVAMGGFDPGAQRTWTFYETIAGGM
                     GGRPGLDGIDGIHCNMTNTMNTPIEAIEQSLPMRFRAYELRPGTGGAGRWRGGCGVRR
                     EWELLAEEATISILAERTRVAPWGLAGGGPGGLARHLVRRADGTEEVLPSKHTTHLRR
                     GDVLVIQTPGGAGYGDPAERDPEAVDRDRERGLG"
     gene            complement(9776..10672)
                     /locus_tag="VEW91_01800"
     CDS             complement(9776..10672)
                     /locus_tag="VEW91_01800"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90349.1"
                     /translation="MAERIGFIGTGAMGAPMAANLLRNGFPVTTIIHRNPLPAQALEA
                     QGARIAPTLSHLLEECPVVLACLPTSAEVEAVVLGPGGVLDRGHRDSVFVDMGTSRPA
                     STRMLAERLQGCGIAMLDAPITGGVRGAREGTLTIYVGGAPGVFARVRPVFEAMGRTV
                     LHMGGTAAGHLTKLLNNMLSLASMAALAEVLPLGVRLGLDPALLVQALAAGSGATAAI
                     PGRGERILRGDFTPSFRVDLAHKDLGLALEVAQEVHLVPPVTQAARMTVTMARSLGLD
                     AEDTVALVKVWERMLGVEVRGR"
     gene            10747..11661
                     /locus_tag="VEW91_01805"
     CDS             10747..11661
                     /locus_tag="VEW91_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HYM90350.1"
                     /translation="MARAMDTTAAVPQASAFDWRRTIAWIDQNGNETERARLRGLLGR
                     PRPEAKVIRTLEARQNDDGGFPAELVQGRPSSIDTTAFVLRWMQDLRMLASGQAQRAV
                     IYLFTVQRPDGSWDEPPGLLKYTPPPRLLPGDTRVQALCTAVAAYWLVLLGHRHDHAV
                     SRAMAYLRSRQAADGRFLGYIRTTWTAAALFRLAEGPGSTVCARALDAIGAIGAERWN
                     AGALAGMLNSMAEAGVNTHVPVVGQGLERLRSLADPTGSWPSEDGEFYRVEVTLRALC
                     VLMLYGAVSSAEGSSGRAGEPVRHGGAP"
     gene            11658..11996
                     /locus_tag="VEW91_01810"
     CDS             11658..11996
                     /locus_tag="VEW91_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90351.1"
                     /translation="MSEQVLKQLHGVVTEAMEERRGLVVYSRLQPVEIDRLARRVERE
                     TIEKIRRLLPESTDDQRVAGLQNRLRRMKEELEQLEGLVEIRDQSRQMQNDEIVWLAF
                     EDIAWMLGIE"
     gene            complement(12009..12380)
                     /locus_tag="VEW91_01815"
     CDS             complement(12009..12380)
                     /locus_tag="VEW91_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYM90352.1"
                     /translation="MSLEAGTILEGIVVKITHYGAFVEFPDGKSGLVHISEIADTYVK
                     DVRDYLKEQERIKVKVLGYNEKGKLDLSVKQALDPAERQARMRAKVSFDEKLAKFLKE
                     SEERLLDLKRNTEAKRGGRRR"
BASE COUNT         1767 a         4315 c         4593 g         1946 t
ORIGIN      
        1 acctgcagcg gctgcaacgg gatccccagc ctgaccctcc agccgctcgg gggcagccag
       61 cagcccctcg tcaagggggt cgattcgatg agcatccagt acgtgttgaa ccgcctgtac
      121 tccaccgccc caggcgagtg caacgctcag accggcggga cctcgagcct gtgcgtcgtg
      181 aacctgccca cgcaggcccc gtcgatcgcc ggcgactggc aggtcgtgcg ggcgatcatc
      241 ttcgcgctcg acgcccggtc catcaccaag gtgcgggcct cggggagcgc cgacggctat
      301 ttccacctga gcgacaccgt cgagatctcg ccccgaaacc ttatctacca gcaaacccgc
      361 gtcccctgga cgccgtactg acggcggacg acaagggcgg cacgaggttc acgggggtag
      421 cggaagtgct ctagggggac gtgtccctcg actgaggttc ttgatcctgc ccggcaccca
      481 gcggggcagg atcggctttt tccgccgcgg aatactggcg caatgtcatc cgcaagcgat
      541 ctcaggacag tcaccggcgc gaccccggcc gagatccggc gccggatccg cggtggggag
      601 tggcgggaac cgaccgcggg gctcgcgccg gggtttgccc aggccaacct cgtcgtcgtg
      661 cctcgggaga tcgcgtacga cttcctcgtg ttctgccagc ggaaccccaa accgtgtccc
      721 ctcatcgacg tgaccgatcc ggggagcccc gagccgtcgc acgcggcgcc gggagcggat
      781 cttcgcaccg atctgccgcg ataccggatc taccggcgcg gggaacttga tgcggaagtc
      841 accgagatca cgaggtactg gcggccggac ctcgtcgcgt tcctcctggg gtgctcgttc
      901 acctttgaga ccgcgatgca gcgcgccggc ctccccgtgc gccacatcgc cgaccgccgg
      961 aacgtggcga tgtacattac ctcgatcccc tgccgcccgg cgggcgggct ccacggcccg
     1021 acggtggtga gcatgcgccc cgtgccgaga gaacacctcg cccgcgccat cctcgtgacg
     1081 ggccgctacc cccgggccca cggggcgccg gtccacgtgg gggatcccgc gaagattggc
     1141 atccgcgatc ttgcccggcc ggagttcggg gatcccccga ggatcgagcc cgatgacgtc
     1201 cccgtgttct gggcgtgcgg ggtgaccccg caggcggtcg cgcaggcggc ccgggtggag
     1261 ttgatgatca cccacgcgcc cggccacatg ttcatcaccg acctgttgga cgaggatctg
     1321 gcggccggct gatctcgatc cggagcgcca tggaggtgcg gcatgaggcg gtcctggctc
     1381 acgcgtcgcg aaatgctcaa gcttgccgga ggggcggtgg tcttcgccgg tggagggtgg
     1441 cgggccgggc cggccgcggc ccaggcggag gaggtcaaga tcgggtgcct gtacccgctc
     1501 accgggtcgc tcgctcgcct cggggcggac atgcggaagg ccgtggatct ggcggctgag
     1561 atcgtcaaca cgccccatcc cgaactcgcc ccgctgaccc tcgccgcgac ctccggcctg
     1621 ccccgcctcg gcgggcggaa gatcgggctg gtgtgggccg acgccaaaga tcccgcgacg
     1681 gcccgggcgg aagcggagcg gctgatcgag caggagcggg tcgcggcgct catggggtgc
     1741 tatgcgagcg ccttcaccaa caccgccagc ctggcggcgg agacgcgcgg gatcccgttc
     1801 ttgaacccgg aatcctcctc gccgacgctg acccagcgcg gcttcaagtg gttcttccgc
     1861 accggcccgc acgacgggac cttcacggcg atgttctatc ggatgtttgc cgatctcaag
     1921 cagcgcgggg tccggatcca gaagtttgcg atcctctccg agaacaccga gttcggggcc
     1981 acggcggccc gcgtggaaga agaggcaggc aagggggcag gctaccagat gatcgcccgg
     2041 gagatgtaca cgtcgccgcc cgccagcctg gatgcggagc tggcgcggat ccgcgcggcc
     2101 aatcccgatg tcctgatcgc gaacgggtac ctcatcgatg cggtgaccac gatcaagacg
     2161 ctcaggacga tgcggtattt tcccccgggg cttgtcgtcc aggactccgg gtacgtcgtg
     2221 ccggactacc tcaaggagac cggcaaggac ggtctttacg tcatcagccg gctcgcgtgg
     2281 gcgctcggac tcggcgtgcg caagcccctg gtcaagaaag tgaacgatct ttaccggagc
     2341 cggttcaacg aggacatgga tgagaccaac gcccggagct tcaccggatt gctcgcgctc
     2401 gccagcgcga tcaatcaggg tggcggcgta acatcgaccc agatcctcca agggctgctc
     2461 cagaccaaca tccccggatc cgatctgatc atgccctggc aggggatcaa gttcgaccag
     2521 gacgggcaga acgtgtacgc cctcggggtc atggggcagc ggctggggga cggagggttc
     2581 aaggtcgtct ggccgttcga tctggcggag accaacatcg tctggcccgc tccggcctgg
     2641 gacaagcgct gaccccccgg ccgcgcatct gcctgcggca ggccgccccc cacccgcccg
     2701 gacgcgccgg ggccgggtag aaccggacgt ctcggtggat ctcctcctcc ccgcatcggt
     2761 cgccggcctc gtgatgggct gcatctacgc gctcgtctcc ctggggctgg gcctgatctg
     2821 gggcgtcatg gacgtcgtca acttcgccca cgccgagtac atgatgctcg cgatgtatgc
     2881 cacctactac gcctgggcgt ggctgcacct ctcccccttc gccgccctcc ccgccgtgat
     2941 cgcgctcggg ttcggcgccg gggtggccac ctatctcctc ctgatccgcc ggatcacggg
     3001 ggcgccgatg gtcacccaga tcttcgccac gttcgggctg ctgtttttcc tgcgctacct
     3061 ggccttcgcg gtcttcgggc cggactttcg ggggatcgcc tgggaaccgg cgaacggcag
     3121 catcgagatc ctcggcgtcc cggtcagcac cgccaaggcc tgggccgcgg gggcggccct
     3181 gctgaccttt gcggcgctcc atgggttcct ccagtacaca aaggtgggca aagcggtcag
     3241 agccacggcg cagagcccgg gggccgcgct ggccctcggg atcgaggtgg agcggatcta
     3301 catgctggcg tggggcctga gcattgcgac cgcagcggtg gccggggtgt tcctcctgcc
     3361 gttctaccag gtgagcccca ccgtggccga cgcgtttctc ctgctgacct tcgccagcgt
     3421 cgtcctcggc ggcttcggca gcctcctcgg gccgctgatc ggtggcgtgg ccatcgggct
     3481 gattgaaggg atcgccggca ccctgctctc gccgacgttc aagctcgtgt tcgtgtttgc
     3541 cgtgttcttg ctcgtgctct tcgttcgccc gagggggctc ctcggagagt ggggcgcgtg
     3601 agcgggatcg cccggtgggc gtggatcgcg gtctggggcg cgctcgtgct gctgccccag
     3661 gcgatacata acacgtatct gctcaacggc ctgatcctgg tcttcatcta cggcgccgcc
     3721 gcgcaggcgt ggaacctcct gggcggctac gccgggcagc tttcgttcgg gcacgtcgtg
     3781 ttcttcgggc tgggcgccta cacgagcacg ctcctgctga tccgactggg actgagcccg
     3841 tggttcggaa tgtgggtcgg ggccggggtg gccgtgctcg tcgcactcgc cgtcggctat
     3901 cccacctttc gcctccggcg ccattacttt gcgctggcga ccctggcgct cgcggagatg
     3961 gcccggatcc tgttcctcaa ctggccgctc gtgggcgcgg cggtgggcct gttcctgccg
     4021 gtccggctga tgaacgcccc ctggacgatg atgtggcagg cgaagccccc ctactattat
     4081 ctggcgctcg cgttgtttgg ggctgcctgc ggggtggtgc tgctgatcga ccgggcgagg
     4141 ctgggcctct acctgcggac gatcgatctc gacgaagacg cagcctcggc gctcgggatc
     4201 cccacgcgcc ggtacaagct gctggtgatc gcgatgagcg cggggctcac cgcgctcgcg
     4261 ggcacgctgt tcgcccagta cgtgctgtac atcgacccgt cctcggtgct ggatcccgcg
     4321 cgctcggtgc tgttcgcggt gatggccctc ctcggcggcc gcgggacggt cgcggggccg
     4381 gcgctgggcg cgggcttcct ggtgctgctc tcgcagtacg ccggcgggac gctcggcgga
     4441 ttgggccggg gatacgacta cgtcatctac gggctggcga tcatgctcgt cgcgctctac
     4501 gagccccggg ggctcgtggg gctcatccac cggctcggcg gccgccgcgg ggccgcgccc
     4561 ccggcgagcg ccggggcgcc gcgggccgaa gggcgggagc ccgcgcggcc ggcccgggcc
     4621 ggagccgccc cgccgctcct cgaggtcgcg ggcctcgcga agcattttgg gggcgtggag
     4681 gccctgcggg gcgtcgatct cgaggtgggg cgcggggaga tcgtcggcgt gctgggtccg
     4741 aacggcgcag gaaagtccac gctctttgac tgcgtgaccg ggttcctccg gccctcgggg
     4801 gggcgcgtcc tgctccgcac agccgccggc gcgcgagcgc tcaccggccg tcccccgcac
     4861 tggatcgcgc agcaggggct ggccaggacg ttccagttga tccgggtgtt cccgtcgctc
     4921 acgacgtggg agcacctgct cgtcggccag gagcacaggg gcgagccgct gctggccgcg
     4981 ttctccgcga gcccgcccga ggtgcgggag caggcgcgcg cgttgatgcg gctcgtgggc
     5041 ctccaggacg cgcgcgacac gcccgccggg gcgctgtcgt acggccagca gaagctgctc
     5101 gcgttggcga cggccctgat gcgccgtccg tccctggtgc tgctggacga gccggttgcg
     5161 ggcgtcaacc ccgcggtgat cgagcggctc aaggcctaca tccgcggcga gaacgccggg
     5221 ggggcgacgt ttctcatcat cgagcacaac atgcaggtgc tgatggagct cgcggagcgg
     5281 ctgtatttca tggcggacgg ccggatggtt gtcgagggcc ccccccaggc gatccaggag
     5341 aacgcccagg tgctggcgat ctactatggc cggtgacccg ccgttgctgg aggtcgcgga
     5401 tgtccacgcg gggtacggcg agcaggagat cctgcgcggg ctctcgctcg tggtccgccc
     5461 gggggaggtc gtgtgcgtga tcgggccgaa cggggccggg aagtcgaccc tcgtgcgcac
     5521 ggtcttcgga tttctccgcc ccctccgcgg gacgatccgg ttcggcggcg aatccattgg
     5581 gggacgccgg ccgccggagc tgctccgccg ggggatcacc tacgtgcccc agggggtcag
     5641 cgccttcctc aacctgagcg tggaggacaa cctgtacctg gccgcctaca cccgggggga
     5701 ccgggacgtg cggcacgaca tcgagcagct gctccagctc tttccggcgc tggccgccgg
     5761 ccggcgacga ccggccagga cgctcagcgg cggggagcgc cggatgctgg agatcgcccg
     5821 ggtgctgctg ctccacccgc ggctcgtgct cctcgacgag ccgacgatcg ggctcgcggc
     5881 ggcgacgatg gcgttcgtct acgagaagat ccgggagatc cagcggcggg gggtggcgat
     5941 cctgctggtc gagcagaacg cgcgcaccgc gctcaagaac agcgaccggg cctacgtgct
     6001 ggagcaggga cgcgtgcggt tcgaggggag cggggaggag atcctcgcgc atcccgaggt
     6061 ccggcgcgcc tacctggggg gataacgcgt ggcgcactat cggatcggcg tcgacgtcgg
     6121 gggcacgttc accgacctca tcgcggcgga ggagggcgcc ggcacgctcg tgccggtcaa
     6181 ggtcctgagc accccgcggg cgcccgccga cggcgtgctc gccgcgttcc accagttcct
     6241 cgagggccgt cccggccggc cgacgtatct cgtgcacgcc tcgacgatcg ggacaaatct
     6301 gttcctcggg caggtggggc tcgagctgcc ccgggcggcc ctgctcacca ccgaggggtt
     6361 ccgggatgtc ctcgagatcg gccggcagcg ccgcgccgag ctgtacaacc tcttctacca
     6421 gcgcccgcgg ccgctggtcg accgccggtt ccggatcggg atccccgagc gcatcgatgc
     6481 gaccggccgc gtggtgcgcc cgctcgacga gggggcggta ggtaccgccg ccgcccggct
     6541 ggcccgggag cggatcgagg ccgtcgcgat cgcgttcctc cacgggtatg ccaaccccag
     6601 ccacgagcgg cgggccaaag cgattctcca ggccgcgctg cccgaggccg tcatcgtggc
     6661 ctcccacgag gtcgatcccg agtaccgcga gtacgagcgg accagcacca ccgtgatcag
     6721 cgcgctcctc tcgcccgtgg tgtcgcggta tctgcgcggg ctctcttcgc ggctgcgcga
     6781 cgagggccac gccctcccac tgtatgccat gcagtcgagc ggggggatca ccacgatcga
     6841 gggcgcggcc gccacccccg cgacgctcat tgagtccggc cccgccgccg gcgtggtggg
     6901 ggcggcggcc ctcggccggc ggctcgggta cgtgcgcgtg ctcagtttcg acatgggcgg
     6961 gacgaccgcg aaggccggcg cgatcatcgg gggccgcccc gaggtcgtgg cggagtacga
     7021 ggtgggcggg acggtccacg ccgggagaat cgtccgcgcg agcggatacc cggtgcgcgc
     7081 gccgtttatc gacctcgcgg aggtcagcgc cggcgggggt acggtcgcgt gggtcgatgg
     7141 gggggcgctc cggtgcgggc ccgtgagcgc gggcgcggat cccggtccgg catgctacgg
     7201 gcggggaggg gaccgcccca cggtgaccga tgcgcacctc ctgctcggct ggctgtcccc
     7261 ccggggcctg ctcgggggcc gcatgccgct ctctgcggaa gcggcggcgc gcgcgatggc
     7321 gtcggtggcc cagccgctgg gtctcgatca gacggccacc gccgccggca tcgtccgcgt
     7381 ggtcgacgcc cagatggtcc gggccctccg gatcgtctcg ctggagcgcg ggtacgaccc
     7441 gcgggagatg gtggcgatcg cgttcggcgg ggccggcccg atgcacgccg ccgccctcgc
     7501 ggatgagctg ggggtccgag aggtcgtgat cccaccaaac ccgggcctgt tctccgcggt
     7561 gggcctgctg gcggcggacc tgcggtgcga tcgggtcgag agcgtgatgc ggccggtcga
     7621 gcaggtcgat cccgactcgt tggaaacccg ctttcggcgc ctcgaggccg cggcacagga
     7681 gggcgtgtcg gacggccagg gggggcggga ggtcgtggtg cggcgcgccc tcgatctccg
     7741 gtatatcggg cagtcctacg agctcaccgt gccgggcgag gtgccgcttg gggccgccgc
     7801 ggaggcgttt cgagttcgcc acgccgagat ctacgggtac gcgccgcccc gggagccggt
     7861 ggaggtggtc aacgcgcgcg tgaccgcgct gggcatcatg cccccgctct cgctgtggga
     7921 gccgccgccg ggcccgcgct cgcccgatcg cggggaggtg cgccgcgcat acttcgaagg
     7981 ccggtgggtc gacgtcccgg tggtggcccg gaaggggctg ggcgccgggg gcgcgattcc
     8041 ggggccggcg atcgtggaag aggacgaggc gacgacgatc gtgccccccg gatggtccgc
     8101 cagaatcggc gaggccggga ccctgcacct gcgtcggggt gcggcatgat ccccgatccg
     8161 gtgacggtgg aggtgatccg caacgcgatg atctatgccg ccgaggagat ggggatcgcg
     8221 ctccgcaacg ccagctactc gcccaacatc aaggagcgga tggatcactc gtgcgcgctg
     8281 ttcgatgcgg cggggcgcct catcgcccag gccgagcata tcccggccga tctgggctcg
     8341 ctgccctggg gggtgcgggc cacgttggcg ctgctgcaac accggccccc gcgcccgggc
     8401 gatctcttca tcctcaacga tccgtacatc gccgggacgc acctgaacga catcatgctc
     8461 gtccgcccgc tgttctggca ggggaccatc gtgggctaca gcgtgaacaa ggcgcaccac
     8521 gtggatgtgg ggggccgcac ccccggcagc ctcagcagcg atgcccggga actctgcgaa
     8581 gaggggatcg tcattcccct cctgccgatc ctccgcgagg gcgtggaggt gccggagacg
     8641 gtggaggtga tcctcgccaa cgtccggtca cccgatacga cgcgggggga tctccgggcg
     8701 cagatcgcgg cggcctcgtt gggggagcgc cgggtcgccg agctgctgga ccggtacggc
     8761 cgcgaggccg tggaagccgc ctgggaagcg atcctcgatc agggggaagc ctccgcgcgc
     8821 cgcgcgttcg ccgtgctgcc cccgggccgg tacaccgccg aggactgtct ggaggacgat
     8881 ttggccgatg gagggctccg ctggatccgg gtcgccgtca tcgtggcgcc cggcgcgatc
     8941 acggtcgact tcgccggcac ggacccccag gtgccgcggc cgatcaacgc gatcctcggc
     9001 gtcaccaatg ccgcggtgct gtacgcgctg aaggccgtct gcgatcccga ctcggtgatg
     9061 aacgacggct ggctccgacc ggtcacgctc cgggttcccc ttggcaccgt gatcaacccc
     9121 gtgagacccg cccccgtggg ggtcggcaac acggaaacca accagcgtat cgccgacacg
     9181 gtcctccggg cgctggccaa ggcggccccc ggccgggtgc cggccgccag caacgggtcg
     9241 atgaccaacg tagccatggg cggcttcgat cccggcgcgc agcggacctg gaccttctat
     9301 gaaacgatcg ccggagggat gggaggccgg cccggtctcg acgggatcga cgggatccac
     9361 tgcaacatga ccaacacgat gaacaccccg atcgaggcga tcgagcagtc cctcccgatg
     9421 cgttttcggg cgtacgagct gaggccgggg accggggggg cggggcggtg gcgcggcggg
     9481 tgcggcgtcc gccgggagtg ggagttgctg gcggaggagg ccaccatctc gatcctcgcc
     9541 gagcgcaccc gggtggctcc ctgggggctc gccggcggcg ggccgggggg tttggcccgc
     9601 cacctcgtcc ggcgggcgga cggcaccgag gaggtgctgc cctcgaagca caccacacac
     9661 ctgcggcgcg gggacgtcct ggtgattcaa acgccggggg gcgccgggta tggggatccg
     9721 gccgagcggg accccgaggc ggtcgaccgg gaccgggagc gcggcctagg ctgactcagc
     9781 ggccgcgcac ctccaccccc agcatccgct cccacacctt gacgagggcc acggtgtcct
     9841 cggcgtcgag accgaggctg cgcgccatcg tgaccgtcat gcgcgccgct tgggtcaccg
     9901 gcggcacgag gtgcacctcc tgcgcgactt ccagcgcgag cccgaggtcc ttgtgcgcaa
     9961 ggtccacccg gaacgacgga gtgaagtcgc cccggaggat gcgctcgccg cgcccgggga
    10021 tcgccgcggt cgccccggag ccggcggcga gggcctgcac gaggagcgcc ggatccagcc
    10081 cgagccggac gcccaggggg agcacctccg ccaaggccgc catcgaggcg agggagagca
    10141 tgttgttcaa gagcttggtc agatggcccg cggcggtgcc gcccatgtgc aacaccgtcc
    10201 ggcccatcgc ctcaaacacc gggcgaacgc gggcaaacac ccccggggca cctcccacgt
    10261 agatcgtgag cgtgccctcc cgggcgcccc gcaccccgcc ggtgatcggc gcatcgagca
    10321 tcgcgatccc gcatccctga agccgctctg ccagcatccg cgtggaggcg gggcggctgg
    10381 tgcccatgtc aacgaagacg ctgtcccggt gccccctgtc caagacgccg cccggcccga
    10441 ggaccaccgc ttcgacctcg gcggaggtgg ggaggcaggc cagcaccacc gggcactcct
    10501 cgagcaggtg ggacagcgtc ggggcgatcc gggcgccctg cgcctcgagg gcctgcgccg
    10561 gcaggggatt gcggtggatg atcgtggtca cggggaaccc gttccgcagc aggttggcgg
    10621 ccatgggggc tcccatcgcc cctgtgccga tgaagcctat gcgctcggcc atgcggcgca
    10681 tcttctgtcc ggtgggaacg gcttcctctc ccggagaaga tacccgggcg agaggagggc
    10741 cggaagatgg ctcgggcgat ggacaccacg gcggcggtcc cccaggcgag cgccttcgac
    10801 tggcggcgca ccatcgcttg gatcgaccag aacggcaacg agaccgaacg ggcgcggttg
    10861 cggggattgc tcggccggcc ccggcccgag gcgaaggtca tccgcacgct cgaggcgcgg
    10921 cagaacgacg acggaggatt tcccgccgaa ctggtccagg ggcgcccgtc atcgatcgac
    10981 accaccgcgt tcgtgctcag atggatgcag gaccttcgga tgctcgcgag cgggcaggcg
    11041 cagcgcgcgg tcatctatct gttcaccgtg cagcgacccg acggctcatg ggacgagccc
    11101 ccggggttgt tgaaatacac cccgccgccg cggctcctgc cgggagatac ccgggtgcag
    11161 gccctctgca cggcggtggc ggcgtactgg ctggtgctgc tgggccaccg ccacgatcac
    11221 gccgtgtccc gggccatggc ctatctccgc tcacggcagg cggccgatgg gcgattcttg
    11281 gggtatatcc ggaccacctg gacggctgcg gcgctgtttc ggctggccga ggggccgggg
    11341 tcgaccgtct gtgcccgggc gctcgacgcc atcggcgcca tcggggccga gcggtggaac
    11401 gccggcgcgc tcgccgggat gctgaacagc atggcggagg ccggggtgaa cacccacgtc
    11461 cccgtcgtcg ggcagggcct ggagcggctg cggtcgctcg cggaccccac cgggtcctgg
    11521 ccgtcggagg atggcgagtt ctaccgcgtc gaggtgacgc tccgagcgct gtgcgtgctc
    11581 atgctctacg gggcggtttc atccgcggaa ggctcttcgg gccgcgccgg cgaacccgtg
    11641 cgtcacggag gagcaccatg agcgagcagg tgctgaagca gctccacggc gtggtgacgg
    11701 aagccatgga ggaacgccgg ggcctggtgg tctactcgcg gctccagccg gtggagatcg
    11761 atcgtctggc ccgccgtgtc gagcgggaga cgatcgagaa gatccgcagg ctccttcccg
    11821 agtccaccga cgatcagcgg gtcgccggat tgcagaaccg gctccggcgg atgaaggagg
    11881 agctcgagca actcgagggg ctggtcgaga tccgggacca gagccggcag atgcagaacg
    11941 acgagattgt gtggctggcg ttcgaagata tcgcgtggat gctgggaatc gaataacccg
    12001 acggtcggtt agcggcggcg gcccccgcgc ttggcctcgg tattgcgctt cagatcgagc
    12061 aggcgctctt cgctttcctt caagaacttg gcgagcttct cgtcgaacga gaccttggcc
    12121 cgcattcggg cctggcgttc cgccgggtcg agagcctgtt tcaccgagag gtcgagcttg
    12181 cctttttcat tgtagcccag gaccttgacc ttgatgcgtt cctgttcctt cagatagtct
    12241 cgaacatctt tgacgtaggt atcagcaatc tcggagatgt gcacgaggcc gctcttgcca
    12301 tcggggaatt ctacgaacgc gccgtagtgg gtaatcttga cgactatacc ttccagaatc
    12361 gtccctgcct ctagactcat acccgagacg tgcccctccc gcaagagatg tgtcggcatt
    12421 atagcctacc ctcccggggg tgtcaatgca gcgcgatcgc acgcacgtgg aggtgcgacg
    12481 cgggtgccac cgaacgcccg tgcgggatcg cccaggggcc tcgatcatgg cgcacgcggg
    12541 ggtcacctgc cgaggacgtg ctcgagcagc gtgaggacgt gcccggctgc gtcccggatg
    12601 gcccgcccga tcccatggga g
//
LOCUS       DASQGH010000094         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42824, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000094 DASQGH010000000
VERSION     DASQGH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42824"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VEW91_01820"
     CDS             <1..437
                     /locus_tag="VEW91_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM90353.1"
                     /translation="SQLPLVLLHNRFAVIVGMVHYLLPYAVFPIFATMSSIDDRLERV
                     AASLGASRAAAIRLVILPLTLPGVYAAALLVFIISTGFFITPVILGGPREMMAANLVD
                     YYARQLVDFRAASVLAAMIITGVSLLVAVYQWLPKEGQFGRL"
     gene            424..1338
                     /locus_tag="VEW91_01825"
     CDS             424..1338
                     /locus_tag="VEW91_01825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90354.1"
                     /translation="MGGSEEALAVSVRRWPVLGRRRWPFSPGGTLLLAASAPIFLYLL
                     LPTLVVVPMALTPKPLLEFPPSGLSLRPFRDLFHDAAWMSAGVVSVKVALLAVSIACV
                     VATMAALALHSARFAGKGIIQAIILVPIVAPLIVLALADYEFLVNFHLTGTWVGIGLS
                     HSVLATPYFFVALQASLAGLDPALVRSARSLGAGTMSVFRHVYLPALRPGLAAGALFA
                     FIASFDETVIALFQQGPQATTLPVKMFTDIQYELSPKIAAVAALLVGLATLVFLTQTA
                     ILLGRRSQAQAPAPAGGRRPRAERGRVG"
     gene            1413..2486
                     /locus_tag="VEW91_01830"
     CDS             1413..2486
                     /locus_tag="VEW91_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90355.1"
                     /translation="MEILSLSKSYGGPDVVGDITFTLKQGEFLTLLGPSGSGKTTTLL
                     MVAGFERPTRGDIRIGGRSIVDDPPQRRNFGIVFQSYALFPHMSVIENVEFPLRMRGM
                     PRRERRARAADMLDKVGLAAFEGRKPRELSGGQQQRVALARALIFDPDALLLDEPLGA
                     LDKRLRESMQIEIKSIQRRIGISVLYVTHDQEEAMVMSDRIAIMRDGHTLQIGTPADL
                     YNRPETPFVATSLGETNLVPCLGVEGDASDRRVVFPDGSRGWARHARAGWDGTSGRRV
                     MISVRPERVRILPPGEHLENSIEGVVRDQTFVGSYIRCTIQAFGQEIIMKGGDDFFTA
                     MAQPGQRVRIGWRKDDAQILYEE"
     gene            complement(2474..2800)
                     /locus_tag="VEW91_01835"
     CDS             complement(2474..2800)
                     /locus_tag="VEW91_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90356.1"
                     /translation="MLAEDDCKNSGRISAVAHCPSEPTRFLVLCHRSRRGEYWVSLDA
                     HWALREIAAAHRLTLSQVAAEILERGLRERAETAGRRGAGDPPAAGSGAYASRLSFKD
                     PLSYSS"
     gene            2860..>3857
                     /locus_tag="VEW91_01840"
     CDS             2860..>3857
                     /locus_tag="VEW91_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90357.1"
                     /translation="MAEVHPMARYPVFMRVALVFLGVILAGAPVTLAQMAGPVTRIDA
                     PGATTTWLNGINAAGQIVGCGSDIMGGSSHGFVYAGGRFTTIDVPGAGANGTCANGIN
                     DVGQIVGDYYGPGDFFTGKAHGFMLAAGRFTTIDVPGAAESFANGINNAGQIVGYYED
                     AAKKEHGFMLAAGRFTTIDAPGATETSAYSINNSAQIVGSYKDAAGIPHGFLLAAGRF
                     TTIDVPGVGTDYIGVFGINDAGQIVGHDGRGPYRGFVVKLESRAGVGSTPSARASSVT
                     IATIDAPGAARTAALGINGAGQIVGHFMDAGGKFHGYLRTAGGTFTTFDAPGATRTA"
BASE COUNT          597 a         1261 c         1275 g          724 t
ORIGIN      
        1 tctctcagct tccactggtg ctcctgcaca atcggttcgc ggtgattgtc gggatggtgc
       61 actacctcct gccgtacgcc gtctttccga tctttgccac gatgtcgtcc atcgatgacc
      121 ggttggagcg ggtggcggcg agcctcggcg cgtcacgggc ggcggcaatc cgcctcgtga
      181 tcctgccgct cacgctcccg ggggtgtatg cggccgcgct gctcgtgttc atcatctcca
      241 ccgggttctt catcaccccg gtgatcctcg gcgggccccg ggaaatgatg gcggcaaacc
      301 tggtcgacta ctatgcgcgg cagcttgtgg acttccgggc ggcgtccgtg ctcgcggcca
      361 tgatcattac cggtgtgtcc ctgctggtgg ccgtgtacca gtggcttcca aaggagggtc
      421 agtttgggcg gctctgagga agcgctcgcg gtctccgtcc ggcgatggcc cgtgctgggg
      481 cggcggcggt ggcccttctc gcccggcggg acgctgctcc tcgcggccag cgcccccatc
      541 ttcctgtacc tcctgctccc gacgctggtc gtcgtcccca tggcgctgac ccccaagccg
      601 cttctggagt ttccccccag cggcctgtcc ctgcggcctt ttcgggatct cttccacgac
      661 gccgcctgga tgagcgcggg cgtggtctcg gtcaaggtag ccctcctggc cgtgtcgatc
      721 gcctgcgtcg ttgcgacgat ggcggcgctc gccctgcact ccgccaggtt cgccggaaag
      781 ggtatcatcc aggccatcat tctggtcccg attgtggcgc cgctgatcgt gctggcgctg
      841 gcggattacg agtttctggt caatttccac ctgaccggca cctgggtcgg catcgggctc
      901 tcacacagtg tcctggccac cccgtacttc tttgtggcgc tccaggcgag ccttgccggg
      961 cttgatccgg ccctcgtcag gtctgcgcgc agcctgggcg ccgggactat gtcggtcttc
     1021 cggcacgtgt acctgcccgc cctccgcccc ggcctcgccg cgggagcgct ctttgcgttt
     1081 atcgcctcgt tcgacgaaac cgtcattgcg ctcttccaac agggccctca ggcgacaacg
     1141 ctgcccgtca agatgtttac ggacatccag tacgagctgt cgcccaaaat cgccgcggtg
     1201 gccgcgctgc tcgtcggcct cgccaccctg gtgttcctca cgcagaccgc catcctgctc
     1261 gggcggcgct cacaagcgca ggcgcccgcg ccggccggcg gacggcgtcc ccgggcggaa
     1321 cggggtcgcg taggatgaaa cgcgggccga tcctgccctt cttgcagaaa cgcggtgccc
     1381 cggcttcaca cacggcgggg agccgggaag acgtggagat cctctccctc tcgaagtcgt
     1441 acgggggccc ggacgtcgtc ggggacatca cgttcacgct gaagcagggc gaatttctga
     1501 cgctgctggg cccgagcggc tcgggcaaga ccacaacgct gctgatggtg gccggtttcg
     1561 agcggccaac ccggggggat atccgcatcg gcgggcgctc gatcgtcgac gatcctccgc
     1621 agcgtcgaaa tttcggtatc gtcttccaaa gctatgcgct gtttccgcac atgtcggtga
     1681 tcgagaatgt ggaattcccc ctccgcatgc ggggcatgcc gcgacgcgag cgtcgcgcgc
     1741 gggccgccga tatgctcgac aaggtcgggc tggcggcctt tgaggggcgg aagccgcgcg
     1801 agctgtcggg cggtcagcag cagcgggtgg cgctcgcccg cgcgctcatc ttcgatccgg
     1861 acgcgctgtt gctcgatgag ccgcttggcg cattggacaa gagacttcgg gagtccatgc
     1921 agatcgagat caagtcgatt cagcggcgga tcggaatttc cgttctctac gtgacccatg
     1981 atcaggaaga ggcgatggtg atgtccgacc gcatcgcgat catgcgggac ggacataccc
     2041 tgcagatcgg cacgccggcc gacctctaca accggcccga gacgcccttc gtcgccacct
     2101 ccctcggcga gaccaacctg gtgccgtgcc tcggcgtgga aggcgacgcc tcggacaggc
     2161 gtgttgtctt tcccgatggc tctcgaggct gggcccggca cgcccgggcg gggtgggacg
     2221 ggaccagcgg gagacgcgtg atgatttccg ttcggcccga acgggtccgc attctgccgc
     2281 cgggggagca cctcgagaac tcgatcgagg gagtggtgag agaccagacg ttcgttggat
     2341 cttacatccg ttgcacgatc caggcgttcg ggcaggaaat catcatgaag ggcggggacg
     2401 acttcttcac cgcgatggcg cagcccggtc agcgcgtcag gattggctgg cgcaaagacg
     2461 acgcgcagat tctctacgag gaataggaca ggggatcctt aaagctcagt cgagaggcat
     2521 acgctccgga tccagccgcc ggaggatctc cagcgccccg tcggccggcg gtctcggcgc
     2581 gctcgcgcag gccgcgctcg aggatctccg cggcgacctg gctcagggtg aggcggtgcg
     2641 cggcggcgat ctcgcggagc gcccagtggg cgtccaggct gacccagtat tcgccgcgcc
     2701 gcgaccggtg gcacaggact aagaaccttg tcggctcact ggggcaatgc gcgaccgccg
     2761 aaattctgcc gctgttcttg cagtcgtctt cggcgagcaa attctccctg ggatgactac
     2821 ccactcctag accggggcat tttatcatca cgagggttga tggcggaggt gcacccaatg
     2881 gctcgatatc cagtattcat gcgagttgct ctcgttttcc tcggcgtcat cctggccggg
     2941 gctccggtca cgctggcgca gatggccggg ccggtcactc ggatcgatgc gcccggcgcc
     3001 accactacgt ggctcaacgg gatcaacgcc gcgggccaga tcgtgggatg cgggtcggat
     3061 atcatgggcg gtagcagcca cgggttcgtg tacgctgggg ggcgattcac cacgatcgat
     3121 gtccccgggg ccggcgccaa cgggacctgc gccaacggca tcaatgatgt cggtcagatc
     3181 gtaggagact attacggtcc cggagacttt ttcaccggga aagcgcatgg cttcatgctg
     3241 gccgccggac gctttacgac gatcgacgtt cccggcgccg ccgagtcctt tgccaacggc
     3301 atcaacaacg ctggtcagat cgtaggatac tacgaggacg ccgccaagaa ggagcacggg
     3361 ttcatgctgg cggccgggcg ctttacaacg attgacgccc ccggcgccac agagaccagc
     3421 gcctacagca tcaacaactc cgcgcagatc gtggggagct acaaggacgc cgcaggcatt
     3481 ccccacgggt tccttctcgc tgcggggcga ttcacgacga tcgatgtccc tggggtggga
     3541 actgattaca tcggtgtctt tggaatcaac gacgccggcc agattgtggg acacgacggc
     3601 cgtggcccgt accgcggctt tgttgtgaaa ctggaaagta gggctggcgt tggctccact
     3661 ccgtcggccc gagcgagttc cgttactatc gccacgatcg atgccccggg cgcggcgagg
     3721 accgctgcac tcgggatcaa cggcgccggg cagatcgtgg gtcacttcat ggacgcgggc
     3781 ggcaagttcc atggctatct gcgcaccgcc gggggcacct tcaccacgtt cgatgcccca
     3841 ggcgcgacga ggaccgc
//
LOCUS       DASQGH010000095         1965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31565, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000095 DASQGH010000000
VERSION     DASQGH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31565"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="dnaN"
                     /locus_tag="VEW91_01845"
     CDS             <1..714
                     /gene="dnaN"
                     /locus_tag="VEW91_01845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYM90358.1"
                     /translation="GLLRTMIRQTSFAVSTDETRPFLTGVYFVIEEKNSRLVATDGGR
                     LALRKATLAGSTKGKVTAIVPSKTMAELVRALGGVEGEVTVSSHENQLVFAVAGMRFV
                     SRLIAGQFPPYEKVIPTEFKQRIKIGTEQLLRAVRRASITARDSANVVRLSADERTLT
                     VSSNTPEVGKAQEDVEVQAEGETIPVAFNAKFLLDALTNMDAPEVFFELTGSLSPGVL
                     RPVDHAEYLYVLAPVRVYG"
     gene            737..946
                     /locus_tag="VEW91_01850"
     CDS             737..946
                     /locus_tag="VEW91_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HYM90359.1"
                     /translation="MALTIFTSEITLGDALKWAGVAPTGGQAKVLIRSGRVLVNGVVE
                     HRRGRRLGPGDRIAVSGREYRVVAR"
     gene            933..>1965
                     /gene="recF"
                     /locus_tag="VEW91_01855"
     CDS             933..>1965
                     /gene="recF"
                     /locus_tag="VEW91_01855"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HYM90360.1"
                     /translation="MWLADLRLRDVRNYQVADLEFGPGTTLIIGANAQGKSNLLEAVY
                     TAALGRSPRAGADAELVRFGQDRAYIRAGVAGARPQVLEVAFDRTTGGRRIKVNGVPA
                     NRGQLLGRCVAVLAGPLDDEMIRGAPVYRRRLMDAALSQASPSYFFALTRYVRVLRQR
                     NELLRAAASVATLAPWDDQLIALGAVLIERRRRFVERLGARAVARHARLGGGEERLEV
                     AYVCAVDPGEERETMRRALEARRGEELRRGTTVVGPHRDDLRLTINGVDVRMFGSRGQ
                     HHTVALSLRLAEVDLLREDLDEWPVVLLDDVLAHLDARRQALLFREVDGPQVLLTHTE
                     LPASREVAMR"
BASE COUNT          306 a          578 c          742 g          339 t
ORIGIN      
        1 gggctgttgc gaaccatgat ccggcaaaca agttttgccg tctccacgga tgaaacacgc
       61 ccgttcttga ccggggtcta ttttgtgatc gaggagaaga acagccgctt ggtggccacc
      121 gatggagggc ggctggcgct gcggaaggca acactggcgg gcagcaccaa gggcaaagtg
      181 acggccattg tgcctagcaa gaccatggcc gagttggtgc gtgccctcgg tggggttgaa
      241 ggtgaggtga ccgtgagctc ccacgaaaac cagctggtgt ttgctgttgc ggggatgcgg
      301 tttgtctcgc ggctgattgc cggacagttc ccgccgtatg aaaaagtgat ccccacggag
      361 ttcaagcagc gcatcaagat cggcaccgag caattgttgc gcgccgtgcg gcgggccagc
      421 atcacggcac gggattccgc gaacgtcgtt cgcctgagcg cggacgagcg gacgctgacg
      481 gtcagctcca atactcctga ggtgggaaag gctcaagaag acgtcgaggt gcaggccgaa
      541 ggggaaacca tcccggtggc cttcaacgcg aagtttctcc tggatgccct cacgaacatg
      601 gatgcccccg aagtgttttt cgagttgacg ggatcgttga gccccggcgt gttgcggccg
      661 gtggaccacg cggagtatct atacgtcctt gccccggtca gggtctacgg atagcggacg
      721 gaccgctcgg gtccccgtgg cgctgacgat tttcacctcg gagatcaccc tcggcgatgc
      781 gcttaagtgg gcgggggttg ccccgaccgg gggccaggcg aaggtgttga ttcgaagcgg
      841 gcgggtcttg gtgaacgggg tggtcgagca ccgtcggggc cgccggcttg gtcccgggga
      901 ccgcatcgcg gtctccgggc gggagtaccg ggttgtggct cgctgatctc agactccgcg
      961 atgttcgaaa ctaccaggtg gcggatcttg aattcggccc cggcacgacc ctgatcatcg
     1021 gggcaaacgc gcagggcaag agcaacctgc ttgaagcggt ttacacggct gccctcgggc
     1081 ggagcccccg ggctggggcc gatgcggaac tggtgcggtt cgggcaggac cgcgcgtaca
     1141 tccgcgccgg cgtagcggga gcgcggcccc aggtgctcga ggtggcgttt gaccgcacga
     1201 ccggcggcag acgcatcaag gtcaacgggg tcccggcgaa ccggggccag ttgttggggc
     1261 gatgtgtcgc cgtgctggcc ggaccgctgg acgatgagat gattcgcggg gcgccggtct
     1321 accggcggcg gctgatggac gccgcgctga gccaggccag tccgtcctat ttctttgcgc
     1381 tgacgcgcta cgtgcgcgtc ctgcggcagc gcaacgagct cctgcgagcg gccgcgagtg
     1441 tggcgacgct ggccccttgg gacgatcagc tgatcgcgct gggagcggtg ctgatcgagc
     1501 ggcggcgccg gtttgtcgag cggctcggcg cccgggcggt cgcgcggcac gcccggctgg
     1561 gcggtgggga ggagcggttg gaggtcgcat acgtctgcgc ggtggatccg ggggaggagc
     1621 gcgagacgat gcggcgggcg ctggaagcgc gccgcggcga ggaactccgg cgcggcacga
     1681 cggtggtcgg gccgcaccgg gacgacctcc ggctgaccat caacggggtc gatgtgcgga
     1741 tgttcggctc ccgcggccag caccacaccg tggcgttgag cctgcggctg gccgaggttg
     1801 acctgcttcg cgaggatttg gatgagtggc ccgtggtgtt gctcgacgat gtcctggcgc
     1861 acctggacgc ccggcggcag gccctcctgt ttcgcgaggt cgacggtcct caggtgctgc
     1921 tgacccatac cgagcttccc gcgtcccggg aggtggccat gcggg
//
LOCUS       DASQGH010000096         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40021, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000096 DASQGH010000000
VERSION     DASQGH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40021"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VEW91_01860"
     CDS             <1..961
                     /locus_tag="VEW91_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90361.1"
                     /translation="RAAAAAGARAVVAALQADLTRQRAVHRREAAHRLLDGLLGGRTV
                     GDLTAQHRQALLELGAIQAQREEPDLVLKQLDPEAFQRCETEAALKRQRLAEAEAALL
                     RLEGRLGTRLPHEDLARVEEEIAETSERLGRHQRLAEVLRLTRAVLFEAHRSTIVPGK
                     AMLEERASRYLRALSDGAYDRIAVDEHSLAPRVWVGPPKEWADVAAREVGSGAVDQCY
                     LALRLALVDVLCPDRRPPLFLDDPFLAYDEHRMDAALRLLREFARDRQVFLLTCRADY
                     HRYADHLIVLGELREAQAPMSATGTAPEAVAAALSLGLGGEDP"
     gene            complement(929..1627)
                     /locus_tag="VEW91_01865"
     CDS             complement(929..1627)
                     /locus_tag="VEW91_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYM90362.1"
                     /translation="MHPDREQRTEAAGGARAPRLRAWGPWIAAGAVLVLAAAAWAVWA
                     HVWVRPSLQGAVLTPPAPTYDFSLRDQDQRLVRLSALRGKAVALTFLYTHCPDICPLI
                     ANKMHETYRQLGPTAARVAFLAISVDPNGDTPESVRTFLSSHRVAGELTYLTGSFAEL
                     RPVWAHYYIGTDAKEVNPGAESVTPPSPDLVSHSAIVYVLDPRGSLRVFLPGNFNPQD
                     LATDLRILASEPER"
     gene            1823..3382
                     /gene="rny"
                     /locus_tag="VEW91_01870"
     CDS             1823..3382
                     /gene="rny"
                     /locus_tag="VEW91_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HYM90363.1"
                     /translation="MTTTNAVVYIGAGVLGLAAFLLGYLLRKFAGEAKIGTAEVAAKG
                     ILEDARSAAEREAESRKREALLEARDEAFQIKRDAEREIREQRAEVQRLERRMIQREE
                     TLDRKLEGAEKRDQATAQREQEVAKAREELQELSQRQRGELERIAGLTVEQARQELFQ
                     KIEVESRRDAVQIAKKIEAEARETAERKARETIALAIQRCAADHTAEVTVSVIPLPND
                     EMKGRIIGREGRNIRALESLTGVDLIIDDTPESVTLSSFDPLRREVAKMALEKLLADG
                     RIHPARIEEMVEKAQRELDTRIQEAGERAAFEAGVHGLHPEEVKLLGRLNFRFSYGQN
                     LLQHSIEVALLAGLMATQLDGDPELARRAALLHDIGKALTHEAEGTHSDLGMDIARRY
                     HEPPEVLNAIAYHHGDAEATCLEAILVAAADAISASRPGARKETVEMYVKRLENLERI
                     ATSFPGVDKAYAIQAGREIRVLVRPQEIDDTNAATMAREMAKRIEEELQYPGQIKVTV
                     LRETRAVEYAK"
     gene            3447..>3939
                     /locus_tag="VEW91_01875"
     CDS             3447..>3939
                     /locus_tag="VEW91_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HYM90364.1"
                     /translation="MNILFIGDIVGKPGRRVLAQMLPGLRREFSVDLAIANGENSAGG
                     FGITRETFEEILAAGVDVVTGGNHTWHAREAVTLLDSDPRLLRPANYPTGTPGRGGGV
                     FHPSGGRSGPAVAVLNLVGRVFMEPLESPFRVGGEQVERLRRETRVLVMAIHAEATCE
                     KAAL"
BASE COUNT          666 a         1266 c         1469 g          538 t
ORIGIN      
        1 ccgcgcagca gcggccgccg gggcccgcgc ggtggtcgcc gcccttcaag cggacctcac
       61 ccggcagcgg gctgtgcatc gccgggaggc ggcgcaccgc ctattggacg gcctgctcgg
      121 gggacggacg gtcggagacc tcaccgcgca gcaccggcag gcgctcctcg agctcggtgc
      181 gatccaggca cagcgggagg agcctgatct ggtcctcaag caactcgatc ccgaggcgtt
      241 ccagcgctgt gagaccgagg ccgcgctcaa gcggcagcgg ctcgccgagg ctgaggcggc
      301 cctgctcagg ctggaagggc gcctcggcac ccggctgccc catgaggacc ttgcccgagt
      361 ggaggaggag atcgccgaga cctctgagcg gctgggccgg caccagcgat tggccgaggt
      421 gctcaggctg acgcgtgccg tgttgttcga agcgcaccgc agcacgatcg tgccggggaa
      481 ggccatgctc gaggagcgcg cgagccggta cctccgcgcg ctctcggacg gggcgtacga
      541 ccggatcgcc gtggacgaac acagcctcgc cccccgcgtg tgggtcgggc cgcccaagga
      601 gtgggccgat gtcgccgcac gcgaagtggg gagcggggcc gtggatcagt gctacctggc
      661 gctccggctc gccctggtcg acgtgctctg tcccgaccgg cggcccccgc tcttcctcga
      721 cgacccgttc ctcgcgtacg atgaacaccg catggacgcg gcgttgcgcc tgctgcgcga
      781 gttcgcccgc gaccggcagg tctttctcct cacgtgcagg gcggattacc atcggtacgc
      841 cgatcacctg attgtcctcg gcgagctacg cgaggcccag gccccgatgt ccgcgacggg
      901 gaccgcgccg gaggcggtag cggcggcgct atctctcggg ctcggaggcg aggatccgta
      961 ggtcggtcgc gaggtcctga gggttgaagt tcccggggag aaacacgcgg aggctgccgc
     1021 gcgggtcgag gacgtacacg atcgccgagt gcgagacgag gtcgggcgag ggcggggtga
     1081 cggattccgc tccagggttg acctccttgg cgtccgtgcc gatgtagtag tgcgcccaga
     1141 ccggcctgag ctctgcaaac gagccggtca ggtaggtgag ttcacccgcc acgcggtgac
     1201 tcgagaggaa cgttcgcacc gactccgggg tgtcgccgtt cgggtcgacg ctgatcgcga
     1261 gaaacgccac ccgcgccgcg gtggggccga gttgccgata ggtctcgtgc atcttgttcg
     1321 cgatgagcgg gcagatatcc gggcagtggg tgtacaggaa ggtcagggcg accgccttgc
     1381 cccggagggc gctgagccgc accagcctct ggtcctggtc gcggagactg aagtcgtacg
     1441 tgggggccgg gggcgtcaac accgcgccct gaagggacgg ccggacccaa acgtgcgccc
     1501 acacggccca ggcggcggcc gcgaggacca gcaccgcgcc cgcggcgatc cagggccccc
     1561 aggcccggag cctcggggcc cgggcccctc cggcggcctc cgtgcgctgc tcccgatcag
     1621 ggtgcatcac accactcctt gcccctcccc cgtgcggggg acaggccgca ttggggggct
     1681 tggtcttcct tgaccccccc atccggtcag gatagaatat atcattgaag gtcgcaatgg
     1741 tcgaacgtat gatcctatcc ctggccacag cggtcggaat ggaggggcgt acgatctagg
     1801 cgacacacgc gagacggagg gggtgaccac cacgaacgcc gtcgtgtaca ttggtgcagg
     1861 cgtcctcggg ttggcggcct tcctccttgg ataccttctg cgcaagttcg ccggcgaggc
     1921 gaagatcggc acggccgagg tcgctgccaa gggcatcctc gaagacgcca gaagtgcggc
     1981 ggagagagag gcggagagta ggaagcggga agcgctcctg gaggccagag acgaagcgtt
     2041 ccagatcaaa cgggacgccg agcgggagat ccgggagcag cgggcagagg tgcagcgcct
     2101 cgagcggcgg atgattcagc gcgaggagac tctcgatcgc aagctggagg gtgccgagaa
     2161 gcgcgatcag gccaccgcgc agcgagagca ggaggtcgcc aaggcaaggg aagagctcca
     2221 ggaactgtcc cagcgccaac ggggtgaact tgagcggatc gccggtctca ccgtagagca
     2281 agcgcggcag gaactgttcc agaagatcga ggtggagagc cgccgcgacg cggtgcagat
     2341 cgcgaagaaa atcgaagccg aagcccggga gaccgcggag cggaaggccc gggagacgat
     2401 cgccctcgct atccagcgat gcgccgccga ccacacggcg gaggtcacgg tctcggtgat
     2461 tccgctgccg aacgacgaga tgaaggggcg gatcatcggc cgggaaggcc gcaacatccg
     2521 ggcgctcgaa agcctgaccg gggtcgacct catcatcgac gacacgcccg aatcggtcac
     2581 gctgtcatcg ttcgatccgc tgcgccggga agtcgcgaag atggcgctcg agaagctgct
     2641 cgccgacggc cggatccacc cggcccggat cgaggagatg gtggagaaag cgcagcggga
     2701 gctcgacacc cgcatccagg aggccggcga gcgggcggcg ttcgaggcgg gggtccacgg
     2761 gcttcatccc gaggaggtga agctcctcgg ccggctcaac ttccggttca gttacgggca
     2821 gaacttgctg cagcattcga tcgaggtggc gctgctcgca gggttgatgg ccacccagct
     2881 ggacggggat cccgagctgg cccgccgggc ggcgctgctg cacgacatcg ggaaggccct
     2941 gacccacgaa gcggagggta cccacagcga cctcggcatg gacatcgccc gccggtacca
     3001 tgagccgccc gaggtcctga acgccatcgc ctaccaccac ggcgacgccg aggcgacgtg
     3061 cctggaggcc atcctggtcg ccgcggccga tgcgatctcg gcgtcgcgcc cgggagcgcg
     3121 caaggagacc gtggagatgt acgtgaagcg gctcgaaaac ctggagcgga ttgcgacctc
     3181 gttccccggc gtggataagg cgtacgccat tcaggcgggc cgcgagatcc gggtgttggt
     3241 cagaccccaa gagatcgacg acacgaacgc ggcgacgatg gcccgggaga tggccaagcg
     3301 gatcgaagag gagctccagt atccggggca gatcaaggtg acggtcctgc gggagacccg
     3361 cgccgtcgaa tacgccaagt agcggccgtt tcgggccacg tgggcgcgac tcgggggagg
     3421 gatcggacgg ccccgggcgt cccgccgtga acatcctgtt catcggggac atcgtcggga
     3481 agccgggacg ccgggttctc gcgcagatgc tcccgggcct tcgccgcgag ttctcggtgg
     3541 atctcgcgat cgccaacggg gagaactcgg ccgggggctt cgggatcacg cgggaaacgt
     3601 tcgaggagat cctggcggcg ggcgtcgacg tcgtgacggg ggggaatcac acctggcacg
     3661 cccgcgaggc cgtcaccctg ctcgacagcg atccccggct gctgcggccc gcgaactatc
     3721 cgacggggac gcccgggcgg gggggcgggg tgttccaccc gtcgggggga cggagcggcc
     3781 ccgcggtcgc ggtcctcaac ctggtagggc gcgtgttcat ggagccgctg gagtccccgt
     3841 ttcgcgttgg aggggagcag gtcgagcgcc tgcgccggga gacgcgggtg ctcgtgatgg
     3901 ccatccacgc cgaggcgacc tgcgagaagg ccgcgctgg
//
LOCUS       DASQGH010000097         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31572, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000097 DASQGH010000000
VERSION     DASQGH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31572"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="VEW91_01880"
     CDS             <1..1633
                     /locus_tag="VEW91_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYM90365.1"
                     /translation="GFKYATRSGMGIAVDDIAIPEQKDKILGTAEKLIDGIEQQFRRG
                     LITSGEKYQRTIDVWTKATEEVTEAMLQNFDTFNPLYMMAQSGARGNIQQIRQLAGMR
                     GLMTDPSGRIIELPIKANFREGLTVLEYFISTHGQRKGLADTAIRTSESGYLTRRLVD
                     VAQDVIVREEDCKTTSGVELGPIMDGNQVVVSMADRISGRVAAEDVVAPRGKRVLVEA
                     GREIEESVAEEIEQAGVPAVSVRSVLTCKVRYGICAKCYGRNLATGNLVEIGEAVGII
                     AAQSIGEPGTQLTMRTFHTGGVAGFDITQGLPRVEELFEARRPKGQAVISELDGKARV
                     SEDKGARTITVSGRDDEREYSVAYGVRVRIADGDRVAAGDQLTEGSVNPHDILRIKGL
                     AAVQNYLVQEIQSVYRSQGVDINDKHIEIIVRQMLRRVKVEEPGDVEFLPGELVDVFA
                     LEEENTRAMAQGKEPATARPVLLGITKASLATESFLSAASFQETTRVLTDAATKGKVD
                     PLLGLKENVIIGKLIPAGTGMPRYRTIKLATEGLS"
     gene            complement(1684..2127)
                     /locus_tag="VEW91_01885"
     CDS             complement(1684..2127)
                     /locus_tag="VEW91_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008388949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYM90366.1"
                     /translation="MILVDANLLLYAYNPSFDRHKQARAWLESVLSRPDPVCLAWATI
                     LAFLRIATNPRAFEYPLSMDEAIPIVSAWLAQPMVTILEPGDRHWAVLTELLSRTQVR
                     GPAVMDAHLAALAIEHGAELCTSDRDFARFPNLRMLNPLEANYKK"
BASE COUNT          503 a          819 c          907 g          406 t
ORIGIN      
        1 gggcttcaag tacgccacgc gcagcggcat ggggatcgcc gtggacgaca ttgcgattcc
       61 cgagcaaaag gacaagatcc tgggcaccgc ggagaagctg atcgacggga ttgagcagca
      121 gttccgccgt ggcctgatca ccagcgggga gaagtaccag cgcacgatcg acgtgtggac
      181 caaggccacc gaagaagtca ccgaggccat gctgcagaac tttgatacct tcaacccgct
      241 gtacatgatg gcccagtccg gcgcccgggg caacatccag cagatccgcc agctggcggg
      301 gatgcggggc ctcatgaccg atccgtccgg ccggatcatc gaactgccga tcaaggccaa
      361 cttccgcgag gggctgaccg tgctggagta cttcatcagc acccacgggc agcgcaaggg
      421 tctcgcggat accgcgatcc ggacgtccga gtcgggctat ctcacccggc ggttggtgga
      481 tgtcgcgcag gacgtgatcg ttcgggagga ggactgcaag accacctccg gcgtcgagct
      541 cggccccatc atggacggca atcaggtcgt cgtctccatg gcggaccgga tctccggccg
      601 cgtcgccgcc gaagacgtcg tggccccgcg cggcaagcgg gtcctggtgg aggccggccg
      661 ggagatcgag gaatccgtcg cggaggagat cgagcaggcc ggggtccccg cggtctcggt
      721 ccggtcggtc ctcacgtgca aggtccggta cgggatctgc gccaagtgct acgggcggaa
      781 cctggcgacc gggaacctgg tggagatcgg cgaggccgtc ggcatcatcg cggcccaatc
      841 gatcggcgag ccggggacgc agctcacgat gcggacgttc cacacgggcg gcgtcgccgg
      901 gttcgacatc acgcagggcc tcccccgcgt cgaggagttg ttcgaggccc ggcgccccaa
      961 gggccaggcg gtgatcagcg agctcgatgg gaaggcccgc gtctcggagg acaagggcgc
     1021 ccgcacgatc accgtgagcg ggcgggatga cgagcgggag tactcggtgg cgtacggggt
     1081 ccgcgtgcgg atcgccgacg gggaccgggt cgcggccggc gaccagctga ccgagggctc
     1141 ggtcaacccc cacgacatcc tccggatcaa gggcctggcc gccgtccaga actacctcgt
     1201 ccaggagatc cagagcgtct accggtcgca gggcgtcgat atcaacgaca agcacatcga
     1261 gatcatcgtc cgccagatgc tccggcgggt gaaggtcgaa gagccggggg acgtcgagtt
     1321 cctccccggc gaactggtcg acgtcttcgc gctcgaggaa gagaacacgc gggccatggc
     1381 ccagggcaag gaacccgcga ccgcgcgccc ggtgctgctc gggatcacca aggcgtccct
     1441 ggcgaccgag agcttcctct cggccgcgag cttccaggag acgacccgcg tgctcaccga
     1501 tgcggcgacg aagggcaagg tcgatccgct gctcggcctg aaggaaaacg tgatcatcgg
     1561 caaactgatc cccgccggga ccgggatgcc ccggtaccgc acgatcaagc tcgcgacgga
     1621 agggttgtcg taagccggag cgctgactga gcctgcccgc gggcggtatc gccgcgggca
     1681 gggctatttc ttatagttcg cctccagtgg gttcagcatt cgaagattgg ggaatcgcgc
     1741 gaagtccctg tcgctcgtgc agagctcagc gccgtgctcg atcgcaagcg cagcgaggtg
     1801 agcgtccatc acggcggggc ccctcacctg tgtccgagac aggagctccg tcaaaacggc
     1861 ccaatgccgg tcccccggct cgaggattgt gaccatcggt tgcgccaacc acgcggaaac
     1921 gatcgggatg gcttcatcca tggagagcgg atactcaaag gcgcgaggat tcgtggcgat
     1981 gcggaggaag gcgaggatcg tggcccacgc aaggcagacg ggatcgggtc tcgacaagac
     2041 gctctcgagc catgcccgtg cctgtttatg gcggtcgaat gatggattgt aggcgtacag
     2101 caggaggttc gcgtcgacga ggatcaccgg tggagagggc cctccaggtg ctctaccaac
     2161 tcgctcgtcc tgtcgtagtc gaggccaggc cgcaccccga gaggtcgagc ctgcacgatg
     2221 aaagggggag gggccttgcg ttcgcgtcgc atcgtgagac ctagacgcag cagctcgttc
     2281 acgatctcct tgaacgagcg tccagaccgg cgtacctcgg tcttgagcct ctccgccaca
     2341 tccggatcaa gcgtcactgt cgtccgcaca tcatgatgct atcatgagtt catcatgatg
     2401 tcaacggcag gctatgaggt aatgaggtag ctggggtagg gagactcgca tgctccaggc
     2461 agcgacgcgc cgaccaaacg catgcttgcc atctccggat taggggcctg gccattgctg
     2521 agcctgaagg aaaacgtgat catcggcaag ctgatccccg ccgggaccgg gatgccccgg
     2581 taccgcacga tcaaactggc gaccgaaggg ttgtcgtaag ccggagcgct gactg
//
LOCUS       DASQGH010000098         5002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_378, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000098 DASQGH010000000
VERSION     DASQGH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VEW91_01890"
     CDS             complement(<1..385)
                     /locus_tag="VEW91_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010281221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYM90367.1"
                     /translation="MWNRTRVLRLLGVFLVSVLVVAEATIAAPGGKYQGVTLRYVAAN
                     HPWTETLKTLIPQFESQTGARVRLESYFEDQLSQKLAIEFASRTSTIDAFMYRPLQEG
                     RQFFVNGWFTDLRPFVENKELTPADW"
     gene            complement(558..1520)
                     /locus_tag="VEW91_01895"
     CDS             complement(558..1520)
                     /locus_tag="VEW91_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HYM90368.1"
                     /translation="MRPSPRTVHWGYFDASLTPVAEVASGETIQLRSVSGAPSDPVPA
                     DWIPPEIPSIYAEVHDRGPGGHLLTGPIHVRGARPKQVLQVDILSVTLGAPYGYNVLA
                     PLKGIFPDAVDRLDRQIIPIDRETGVATIAPGLRLPTRPFFGVLGVAPPSGWGRVDTS
                     PPRCHGGNMDNKELVAGTTLFLPIWAEGALFSAGDGHAAQGDGEVDITAIETSLEGEF
                     RLGIREDFELSLPIAVTPTHLITMGFDPSLDQAARVAVRSLLDVLERFGEMAWRDAYR
                     LASIAADLHVTQVVNGEKGIHVMLERTILEQLGATLPCLSGRPT"
     gene            1812..3095
                     /locus_tag="VEW91_01900"
     CDS             1812..3095
                     /locus_tag="VEW91_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HYM90369.1"
                     /translation="MSMTMAEKILARASGRRVVVPGEFVTGRVDFALLHDIFAASVFD
                     LLRDARVSSVWDPARVAVVIDHLVPAPSAAAAAVHGRIREYVRAFGLDHFFDAGSGIC
                     HQLIAERGLIRPGQLLLGTDSHTTTAGALGAGGTGIGTSEMAFVLATGELWLQVPPTI
                     RVDLVGRLGAGVMWKDVILALARRIGTDGAQYRALEFGGPGAAQADLAGRLTVSNMAV
                     ELGAKFGLFPADGTTLAHFRACGVDADPFGPDADAAYEQVIVAELESLDPQVALPHAV
                     DNVRPVREVEGIAIDQAFIGSCTNGRLEDLRIAAQILAGRRVHPRTRLLVAPASREIE
                     RQAAREGLLNSLLDAGALLLPTGCGPCFGGHLGLLAAGERCIGTHNRNFSGRMGSPQA
                     EIFLASPATVAASALYGRITDPRGVISPAPAPAVG"
     gene            3110..3643
                     /gene="leuD"
                     /locus_tag="VEW91_01905"
     CDS             3110..3643
                     /gene="leuD"
                     /locus_tag="VEW91_01905"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051052.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYM90370.1"
                     /translation="MSTQESHTVIRGRAWSFGDDISTDLLSPGVYAVAPLEERRRHTL
                     EPVNPHFAAGVRPGDIVIAGRNFGCGSSRETAPEELKALGVGCVVAASFARIFLRNAV
                     AIGLPALPCPGAASAVDEGDLVEVDLDAGALRNLRTGIVVTGAPLPPQMVEILRAGGI
                     LALLAHRTRERIFRTAQ"
     gene            3640..4950
                     /locus_tag="VEW91_01910"
     CDS             3640..4950
                     /locus_tag="VEW91_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HYM90371.1"
                     /translation="MIWNPAAETASRQDLAHLQNARVRGVISRVYGTVPFYRARFDAA
                     ALRPEDIRGTGDLPRVPFTTKDDLRRLYPFGFFACPREEVVELHCSSGTTGYPTAAGY
                     TRADLEIWGEVMARSLASGGVRPGDLIQNAYGYGLFTGGLGFHYGAQRLGAGVLPTSS
                     GNTGRQIRLMREFGATVLCCTPSYALHLAEAIEESGEGPVALRCGAFGAEPWCEETRR
                     EIERRLGVRASDFYGLSEVIGPGVAAECEARCGLHVHEDHFLPEIVDPATGAPLPQGA
                     QGELVLTTLTKEATPLLRYRTGDITALASDPCPCGRTLMRMQRVSGRIDDMLIIRGVN
                     VFPSEIETVLMRISELAPHYQLIVDRPRALDVLEVRVEARDGVRPGMVHALGERAARA
                     LRTELGLSAVIAVLPPRELPRSEGKALRVIDRRKGVQRCSGPRN"
BASE COUNT          763 a         1666 c         1690 g          883 t
ORIGIN      
        1 tccagtccgc cggcgtcagc tctttgttct ctacaaacgg ccgcaggtcg gtaaaccacc
       61 cattgacgaa gaactgtcga ccttcctgga gcgggcggta catgaacgcg tcgatcgtcg
      121 acgtgcgcga cgcgaattca atggcgagct tctggctcag ttggtcctca aaataggatt
      181 cgagccggac ccgggcaccg gtctggctct cgaactgtgg aatgagggtt ttgagcgtct
      241 cggtccatgg atggttcgct gcgacgtacc ggagcgtcac gccctgatac tttcccccgg
      301 gagccgcgat ggtcgcctcg gccaccacga gcacgctcac caggaacacg cccaatagcc
      361 tcaacacacg ggtgcgattc cacatacgca aacctccctt taatcgtgca tacgttaggt
      421 ataactgggc ccaatgtcac tcgtcaatgt tcgcataact cgggacgccc tagtatcggg
      481 cggggggccg atcttggggc caagaagagg ctcgtcacga ctgcccgcgc catcgccggg
      541 gggcatatca cgtccctcta cgtcggcctc cccgagaggc acgggagagt cgcccccaac
      601 tgctccagga tcgtccgctc gagcatgacg tggatgcctt tttcgccgtt cacgacctgg
      661 gtgacatgta ggtcggccgc gatgctggcg aggcggtagg catcgcgcca cgccatctcg
      721 ccaaaccgct cgagcacgtc gagcaacgaa cggactgcga cccgggccgc ctggtccagg
      781 ctgggatcga accccatcgt gatcaggtgt gtcggcgtca cggcgatggg cagggagagc
      841 tcgaaatcct cgcggatacc cagccgaaac tccccttcca gcgatgtctc aatggctgtg
      901 atgtcgacct ctccatcccc ctgggcggca tgtccgtcac ccgcgctgaa caacgcacct
      961 tcggcccaga tgggcaggaa cagcgtcgtc ccggcaacga gctccttatt gtccatgttc
     1021 ccgccgtggc atcttggggg gctggtatcg acgcgtcccc accctgatgg cggggccact
     1081 ccgagaaccc caaagaacgg tcgcgtcggc agcctcaggc cgggtgcgat cgtggccacc
     1141 ccggtctcgc gatcaatggg gatgatctgc ctatcgagcc ggtcgacggc gtcggggaaa
     1201 attcccttga gcggagcgag cacattgtac ccgtacggcg cgccgagcgt gacggacaag
     1261 atgtccacct gcagaacttg cttcggccgg gccccccgca catgaatggg gccggtcagc
     1321 aggtgtcctc ccggtccccg atcgtggacc tcggcgtaga tgctagggat ctcggggggg
     1381 atccagtctg ccggcaccgg atcgctggga gcgccggaga cgcttcggag ctggattgtc
     1441 tccccggagg cgacctcggc aaccggtgtg agcgaggcgt cgaagtatcc ccaatgcacg
     1501 gttcgaggcg atggtcgcac gcgctgcact ccgctgatgc ctccttggct accggtgccc
     1561 agatagcggg tgacattact tgatctaggc cgcaggtaag actctctcct tctcgttttt
     1621 ggggagaccc cctttggcag ccgagtccca agaagccccc gtaggggcct ttgcgcaccg
     1681 aagcatttgc actaggagaa atgatgcaaa agcctgattg ctcgggtctc cccgccggcc
     1741 atatcctgag tttaagctaa ccaaccaacc ggtcggacgg acagcggttt cgcccgccgg
     1801 agggctcgcc catgagcatg acgatggcgg agaaaattct ggcccgcgcc tccggccggc
     1861 gcgtggtcgt ccccggggag ttcgttaccg gccgggtcga cttcgccctg ctgcacgaca
     1921 tctttgcggc cagcgtcttt gacctcctcc gtgacgcccg cgtctcttcc gtctgggatc
     1981 cggcgcgggt ggccgtggtg atcgatcacc ttgttccggc gccgtcggcc gcagcggccg
     2041 ccgtccatgg tcgcatccgg gaatacgtgc gagcgtttgg cctcgatcat tttttcgatg
     2101 ccgggtccgg gatctgccat cagctcatcg ctgagcgcgg gctgatccgg ccgggccagt
     2161 tgcttttggg caccgattcc cacacgacga ccgcgggtgc gctgggcgcc ggcgggaccg
     2221 ggatcggcac ctcggagatg gccttcgtcc tggcgacggg ggagttgtgg ctccaggtgc
     2281 cgcccacgat ccgagttgat ctggtcggac gtctgggtgc cggtgttatg tggaaggacg
     2341 tcatcctggc gctcgcccgg cggatcggca ccgacggcgc ccagtatcgg gcccttgaat
     2401 tcggcggccc tggggccgcc caggccgatc tcgccggccg tttgacggtc tcgaacatgg
     2461 ccgtcgagtt gggggcgaag ttcggcctct ttcccgccga cggcacgacc ctcgcgcact
     2521 ttcgagcgtg cggcgtagat gccgacccat tcgggcctga tgccgatgcc gcgtacgagc
     2581 aggtcatcgt ggcagagttg gagtcgctcg atccccaggt cgcgctcccg cacgccgtcg
     2641 acaacgtcag gcctgtccgg gaggttgagg ggatcgccat cgaccaggcg ttcatcggtt
     2701 cgtgtacgaa cggccggctc gaggatttgc ggatcgccgc gcagatcctg gccgggcgcc
     2761 gcgttcaccc gcgcacccgg ctgctcgttg ccccggcctc gcgggagatc gagcggcagg
     2821 cggcgcgcga agggcttctc aattccctgc tggatgcggg cgcgctcctc ctgccgaccg
     2881 gctgcggccc ttgcttcggc gggcatctgg gcctgctggc cgcgggcgag cgctgcatcg
     2941 gcacgcacaa ccggaatttc tccgggcgca tgggcagccc gcaggccgaa attttcctcg
     3001 cttctcccgc cacggtcgcg gcgagcgcgc tgtacggtcg catcaccgat ccgcgcgggg
     3061 tgatctcgcc cgcgccggcg ccggcggtcg ggtgagtggg gggggcgccg tgagcactca
     3121 agaatcgcac accgtcatcc gcggccgggc ctggagcttc ggcgacgaca tcagcaccga
     3181 cctgctttct cccggcgtgt acgcggttgc cccgctcgag gagcgcaggc ggcacacgct
     3241 cgagccggtg aaccctcatt tcgcggccgg ggtgcgaccg ggggacatcg tcattgccgg
     3301 gcgcaacttc ggatgcggga gcagtcgtga aacggcgccc gaggagctca aagccctggg
     3361 agtcgggtgc gtcgtcgcgg cctcgttcgc ccggatcttc ctgcgcaacg cggtggcgat
     3421 cggccttccc gccctgccat gtcctggcgc cgcgtcggcc gtcgacgagg gggatctcgt
     3481 ggaggtggac ctcgatgccg gggcccttcg caacctcagg accgggatcg ttgtgaccgg
     3541 tgcgccgctt cccccgcaga tggtggagat cctgcgggcg ggcggcatcc tggcgctcct
     3601 cgctcaccgc acccgggagc gtatttttcg gacggcgcaa tgatctggaa cccagccgcc
     3661 gagacggcca gccggcagga cctggcgcat ttacagaatg cgcgggtgcg cggggtcatc
     3721 tcgcgcgtct acggaacggt gccgttctac cgcgcgcgct ttgacgccgc cgctctccgt
     3781 cccgaagaca tccgcgggac cggcgacctg cccagggtac cgttcaccac gaaggacgac
     3841 ctccgccggc tctatccctt cggtttcttt gcctgtccgc gggaggaggt ggtggaactc
     3901 cactgctctt cgggcaccac cggctacccg acggccgcgg gatacacgcg cgccgacctc
     3961 gagatctggg gagaggtcat ggcgcgctcg ctcgccagcg gcggcgtgag gccgggagac
     4021 ctgatccaga acgcttacgg gtacgggttg ttcaccggcg ggctgggctt ccactacggc
     4081 gcgcagcgcc tgggtgccgg ggtgctcccg acgtcctcgg gcaacaccgg ccggcagata
     4141 cggctgatgc gggagttcgg cgccacggtg ctctgctgta ccccctcgta cgcgctgcac
     4201 ctcgccgagg cgatcgaaga atccggtgaa gggccggtcg ccctgcggtg cggcgccttc
     4261 ggggccgagc cgtggtgcga ggagacacga cgcgagatcg agcgccgcct cggggtccgg
     4321 gcgtccgact tctacggctt gagcgaggtc atcggccccg gggttgccgc tgagtgtgag
     4381 gccaggtgcg ggctccacgt tcacgaggat cactttctcc cggagatcgt cgacccggct
     4441 acgggcgcgc ccctccctca gggggcgcag ggtgagctgg tcctcactac cctgaccaag
     4501 gaagcgaccc cgctactgcg ctaccgcacc ggcgacatta ccgcgctggc ctccgacccg
     4561 tgcccgtgcg gccgtacgct gatgcggatg cagcgcgtga gtggccgcat cgatgacatg
     4621 ctgatcatcc gcggggtcaa tgtgttcccc tcggaaatcg aaacggtgct catgcggatt
     4681 tcggagctgg ctccgcacta tcagctcatt gtcgaccggc ctcgtgcgct cgacgtgctc
     4741 gaggtccggg tcgaagcccg cgacggcgtg aggccgggca tggtgcacgc gctgggagag
     4801 cgcgccgcaa gggcgctgcg cacggagttg gggctctcgg cggtgatcgc cgtcctgccg
     4861 ccgcgcgagc ttccacggag cgagggaaag gctctccggg tgatcgacag acgcaagggg
     4921 gtgcagagat gctcggggcc gcggaactga tctacgattg gaacacggcg ggcacccatc
     4981 cgcagcggcc agactgcgtc gt
//
LOCUS       DASQGH010000099         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3191, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000099 DASQGH010000000
VERSION     DASQGH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3191"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..332
                     /locus_tag="VEW91_01915"
     CDS             123..332
                     /locus_tag="VEW91_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90372.1"
                     /translation="MWGNERTYRMIAEERIRQYRREADQDRRAEQAAILRGPTRTPIV
                     SLLDGISRMFVHRYLLPLLASVWRH"
     gene            362..838
                     /locus_tag="VEW91_01920"
     CDS             362..838
                     /locus_tag="VEW91_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM90373.1"
                     /translation="MKTLLDWADHVFEREGWHVSLLDSVKGVTARQAAWAPAPERNSI
                     WKIVDHVALWKEEGARRILGETPRQAAWAKEHDWRPIGEVSEARWQGAVRHLMQAHAA
                     VKAAMAERSDADLQPPPAGSAAPLAATMRGLILHDGYHCGQICYLRALQGIPAKIW"
     gene            926..>1617
                     /locus_tag="VEW91_01925"
     CDS             926..>1617
                     /locus_tag="VEW91_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYM90374.1"
                     /translation="MDDRPLELMIPGPVPVSPEVLDAMGQPVRQHYGPEWLPYYQRFI
                     ARLQQLFATTGSVYPIPASGSGGLEAMLGTLIGADGSAGIIVNGFFGNRLLHIARAHT
                     PHVEVLQIPYERPADPEEVRAWLRQHRVRVLAVVHSDTSTGVLNPVGEIAAAARAEGV
                     ALAVDAVSSLGGTPVLTDAWGLAAVSTASQKCLESPPGVAPVAVTKLGWEIIDSQPVP
                     SRGWYLNLRTWR"
BASE COUNT          270 a          524 c          580 g          243 t
ORIGIN      
        1 ctgggagttc aagcgcctga gaaagactcc acccccgacg ctcgcggcgt gagcgggccc
       61 agcacacgtg tgtccggcat gagcacgaaa gagatcacgc aggggaaaga gggaggacga
      121 ccatgtgggg gaatgagcgg acctacagga tgatcgctga ggagcggatc cggcaatacc
      181 gccgcgaggc tgaccaggat cggcgggccg agcaggcagc gatcctccgg ggacccacga
      241 ggacgccgat cgtgagtctc ctggatggga tcagccggat gttcgtccac cgatatcttc
      301 tgccgttgct cgcatcagtc tggcgccatt gagcggaggg agcgccgcga tgggagtcgc
      361 tgtgaagacg ctgctcgact gggcggatca cgttttcgag cgtgaaggct ggcacgtctc
      421 tctgctggat tcggtgaagg gtgtgacagc ccgtcaggcc gcgtgggcac ctgcgcctga
      481 gcgcaactcg atctggaaga tcgtcgacca cgtcgcgttg tggaaggaag agggggcgcg
      541 ccgcatcctt ggggaaacgc cgcggcaggc ggcctgggcg aaggagcacg attggcgtcc
      601 gatcggcgag gtctcggaag cgcgctggca gggggcggtc cggcacctta tgcaggccca
      661 cgcagcggtg aaggccgcga tggcggagcg ctccgacgca gatctccaac ccccgcctgc
      721 cggttccgcc gcgccgctgg ccgcaacgat gcgcgggctc atcctgcacg atggctatca
      781 ctgcggccag atctgctacc tgcgcgcgct ccaagggatc cccgcgaaaa tttggtagac
      841 gtccgttcat catgagcaac cgcgcgcaca tcgcgccggc ggtcacagag gagccatccc
      901 cgcctccacc gaaacggaag actccatgga tgaccgaccg cttgagctca tgatccccgg
      961 gccggtcccg gtcagccccg aggtactcga tgcgatgggg caaccggtcc ggcagcacta
     1021 cggcccggag tggttgccgt actaccagcg gttcatcgcc cgcctgcagc agctctttgc
     1081 caccaccggc tcggtctatc cgatcccggc ctccggcagc gggggcctgg aggcgatgct
     1141 cgggacgctc atcggggctg acggaagcgc ggggatcatc gtgaacggtt tcttcggcaa
     1201 ccggctgctg cacatcgccc gggcccatac cccgcacgtc gaggtgctcc agattcccta
     1261 cgagcgcccg gcggatccgg aggaggtgcg ggcctggctg cggcagcacc gggtgcgcgt
     1321 gctcgccgtg gtgcacagcg acacatccac cggggtgctg aacccggtgg gggagatcgc
     1381 cgccgcggcg cgggcggagg gcgtcgccct cgccgtcgac gcggtgtcct cgctgggcgg
     1441 gacgccggtg ttgacagacg cgtggggtct ggcggccgtc tcgacggcat cgcagaaatg
     1501 tctggaaagc cccccggggg tcgccccggt cgcggtgacc aagctggggt gggagatcat
     1561 cgacagccag ccggtcccgt cgcgcggctg gtacctcaac ctccgcacgt ggcggca
//
LOCUS       DASQGH010000100         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14635, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000100 DASQGH010000000
VERSION     DASQGH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14635"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VEW91_01930"
     CDS             <1..757
                     /locus_tag="VEW91_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYM90375.1"
                     /translation="HTLVVTLRDQTEYLAAELAGAPAPLLLVQPVDRGTAAAVLFAAH
                     RICWWDPEATIALFPSDHFVLEETVFMGHVVNLAAVVRQCPGWCILLGAQPTHPETEY
                     GWIEPGEPLGETASGPLYRVRRFWEKPSLETARACLAAGCLWNTFVMVVKASTLIDLG
                     RQFLPDLHSQFVRIGRFADTEDEPPAIQEAYRVVANADFSHTILGRCPSFLVVSRLPA
                     LTWCDLGTPERVLKSLSRIGVTPPWAAAVESRK"
     gene            892..1119
                     /locus_tag="VEW91_01935"
     CDS             892..1119
                     /locus_tag="VEW91_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HYM90376.1"
                     /translation="MDIKQQLGLPPGKWVVANCGKFPSEKNCKLVIMAPQSQRADLVD
                     AAAAHAVRVHGHKESPQLKKELDGFLVTIDV"
     gene            1286..2683
                     /locus_tag="VEW91_01940"
     CDS             1286..2683
                     /locus_tag="VEW91_01940"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HYM90377.1"
                     /translation="MASGLEPFSPSSQDPWDVAKAGHLLRRAGFGPLPPEIARAVQAG
                     PERAVESLFAFPGDPPPPLPFDEIAPAEARVDEIILESMRTRTRPKDNPELRAAFQQA
                     HQAHARGIVALTAWWLDRMTKSPAPLQEKLTLFWHGHFTTSFGDVHDAIAINNQNQLF
                     RRHAAGNFARLLDGVARDPAMLRYLNNDANRKGHPNENWARELMELFTMGIGHYTETD
                     IRESARAWTGWTLREPRTFEGRRAFEFRPQLHDMGTKTFQGQVGNLDGTDIMRIILAN
                     PVTPRWIAGRLARFFVSPTPDPDLVEAMAQHLFTSRYELAPVLRALFRSRAFYRPDVV
                     HAQIKSPVEFVVGAVRHLGIVDPDWPRLGQLAADAGQRLFFPPTVKGWDGGKAWINAA
                     TVFTRANLAGGFLSGRFGTPEVASLSSLDAMAAQLLQRPLPPARLAILGQAMQGTTPE
                     AAIHLIMSLPEYQVS"
     gene            2697..3986
                     /locus_tag="VEW91_01945"
     CDS             2697..3986
                     /locus_tag="VEW91_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYM90378.1"
                     /translation="MPNMMTRREFLTKGLTIVAAGATAPMFLTRTALALNNPWDQALT
                     ASASGRPDGPILVVVQMGGGNDGLNTVVPFAHDDYYRARPRLAVPRETVLRATDELGF
                     HPGLKPLKALYDEGRLAVVQGVGYPNPNRSHFRSMEIWHTADPSGTGPGTGWLGRLCD
                     SECPDCGPTAGIAMLGAEMPLAMQGGSGKAVVLESPQAFAFHPIQGGGPQEAEAFRRL
                     MQPVPGEEPMVDFLTHTAMDAILASDDIRKVTGRPGADTAPYPGDPFSLKLRLVSELI
                     SAGAPTRVYYVGLGGFDTHAAQAGRHDRLMEQLGAGLAAFVGDLTQKGLLERVLVMTF
                     SEFGRRVAENASGGTDHGAAAPMFLIGSQVAPGVHGVHPSLVDLDQGDLKFGVDFRSV
                     YATVLEQWMGIGSQAILGDRFPAVGILRPRAGLLKAG"
     gene            complement(4057..4563)
                     /locus_tag="VEW91_01950"
     CDS             complement(4057..4563)
                     /locus_tag="VEW91_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90379.1"
                     /translation="MTEARQDRWGQLWQSLAALQQHAAWLRALSQHEPLTQPRPGYPR
                     AGLGRSHAGEVREVLRDIHTRLEEVRTLCPAVLPTGASPAGLAADEAPQEIEKRVDVL
                     SRLASTLEREAFQPLPPLPPHAPPYLVEPPGHDLAGTKAVVLALGIEALVTAVRNAML
                     GAANAGPG"
     gene            5072..5374
                     /locus_tag="VEW91_01955"
     CDS             5072..5374
                     /locus_tag="VEW91_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90380.1"
                     /translation="MTVVREGNATIAGRPAFYRYITLQTKSGDDLYAVMVFFTVGQDG
                     YLIFGVTRNAPETFRRNFAEISRILETFRPIGNPGPSIAPGSKRSTAPAPPAQRAA"
BASE COUNT          865 a         1847 c         1869 g          898 t
ORIGIN      
        1 gcacacgctc gtggtgacgc tccgggacca gaccgagtac ctcgcggcag agttggcagg
       61 cgcacctgct cccctacttc tggtccagcc tgtcgatcgg ggcaccgctg cggctgtcct
      121 cttcgccgcc caccgaatct gctggtggga tcccgaggcc actatcgctt tgtttccctc
      181 cgaccacttc gtcctcgaag aaacggtatt catgggccat gtcgttaacc tggcagccgt
      241 ggtgcgccag tgccccgggt ggtgcattct tctgggcgca cagccgaccc accccgaaac
      301 agagtacggc tggatcgaac ctggagagcc gctgggcgag accgcgtcgg ggcctcttta
      361 tcgcgtccga cggttctggg aaaagccctc gcttgagaca gctcgtgcgt gtcttgcggc
      421 gggatgtctc tggaatacct ttgtcatggt tgtgaaggca tcgacactga ttgatctcgg
      481 gcgccagttt ctgccagacc tgcacagcca gtttgttcgg attggccggt tcgcagatac
      541 cgaagacgaa ccccccgcaa ttcaagaggc ctatcgggtc gtcgcgaacg ccgatttctc
      601 ccatacaatc cttgggcgct gcccgtcctt cctcgtcgtc tctagattgc cggcactgac
      661 atggtgcgat ttgggaacgc ccgagcgagt actcaagagc ttgagccgga ttggggtcac
      721 accaccctgg gccgcagccg tggaaagtcg aaagtaaacc cgtcattccg atggggatgc
      781 cacgccattc ccaatctgca ccgtctcgta ggtgcaggac cccgcgcatc cgtggagaat
      841 atcgggatag tggcctgttt caggtcaact tgagaaagcg aggtgacatg catggacatc
      901 aagcaacaac tgggacttcc cccggggaag tgggtggtag cgaattgtgg aaagtttccc
      961 agcgagaaga actgcaaact cgtgatcatg gccccgcagt cgcagcgggc cgacctcgtc
     1021 gatgccgccg cggcgcatgc ggtgagagtc cacggccaca aggagagccc gcagctcaag
     1081 aaggagcttg atgggtttct cgtcaccata gacgtgtaac agggtagccc cccgcgaggg
     1141 ggacgttggg acatgccgca aggccaggcg ggcgcctggc cttgcggcgt tgagggaact
     1201 tttgtcgcgc ttcgcccgct tgggaggcta acggcgcgcc ggatcggtga ccgccgttgg
     1261 tgcgcgacac acaaggaggt cgttcatggc ttcgggcctt gagccgttct ccccctcctc
     1321 ccaggacccc tgggacgtgg cgaaggcggg gcacctgctg cgccgtgcgg gatttgggcc
     1381 gctcccgccc gagatcgccc gcgcggtgca ggcgggaccc gagcgggcgg tcgagagcct
     1441 gttcgcgttc ccgggtgacc cgccgccccc actcccgttc gatgagatcg cgcccgcgga
     1501 ggcgcgggtg gacgagatca tcctcgagtc catgcggacg aggaccaggc caaaggacaa
     1561 ccccgaactc cgcgccgcct tccagcaggc gcaccaagcg cacgcgaggg ggatcgtggc
     1621 cctcaccgcg tggtggctcg accgcatgac gaagagcccc gcgccgctcc aggaaaaact
     1681 cacgctcttc tggcacgggc acttcaccac gtcgttcggg gatgtgcacg atgcgatcgc
     1741 catcaacaac cagaaccagc tcttccgccg gcacgcggcg gggaacttcg cccggctctt
     1801 ggacggggtc gcccgggacc cggcgatgct gcggtacctg aacaacgatg cgaaccggaa
     1861 gggccacccg aacgagaact gggcccgcga gctcatggag ctcttcacga tggggatcgg
     1921 gcactacacg gagaccgata tccgtgagtc cgcgcgggcg tggaccggct ggaccctgcg
     1981 cgagccccgg acgttcgagg gccggcgggc gttcgagttc cggccccagc tgcacgatat
     2041 ggggacgaag acgttccagg ggcaggtcgg gaacctcgac ggcacggaca tcatgcggat
     2101 catcctggcc aacccggtca ccccgcggtg gatcgccggc cggctggcca ggtttttcgt
     2161 ctcccccacc ccggatccgg acctcgtaga ggcgatggcg cagcacctgt tcacgagccg
     2221 gtacgagctc gcgcccgtgc tccgcgcgct gttccggtcg cgggcattct accggccgga
     2281 cgtcgtgcac gcgcagatca agagcccggt cgaattcgtg gtgggcgcgg tgcgccacct
     2341 ggggattgtg gatccggact ggccccgcct cggccagctc gccgcggacg ccgggcagcg
     2401 gctgttcttc cctccgactg tcaaagggtg ggacggcggg aaggcctgga tcaacgccgc
     2461 gaccgtcttc acccgagcca acctggccgg ggggttcctc tcgggaaggt tcgggacgcc
     2521 ggaggtggcc tcgctctcct cgctcgatgc catggctgcg cagctgctcc agcgccccct
     2581 ccctcccgcc cgcctggcga tcctggggca ggcgatgcaa gggaccaccc cggaggcggc
     2641 gatccacctc atcatgagcc tgcccgagta ccaggtcagc tgaggaggtc tgggcgatgc
     2701 cgaacatgat gacacgacgc gagttcctga ccaaggggct cacgatcgtg gccgcggggg
     2761 cgacggcccc gatgtttctc acgcgcacgg ccctggcgct gaataacccc tgggaccagg
     2821 cgctcaccgc ctccgcctcg gggcgcccgg acggcccgat cctggtcgtg gtgcagatgg
     2881 ggggcggcaa cgacgggctc aacacggtcg tgcccttcgc ccacgatgac tactaccggg
     2941 cgcggccccg cctggcggtt ccccgggaga ccgtcctccg cgccacggac gaactcgggt
     3001 tccacccggg gctcaagccg ctcaaggcgc tctacgatga aggccggctc gccgtggtgc
     3061 agggcgtcgg ctaccccaac ccgaaccgca gccactttcg gtcgatggag atctggcaca
     3121 ccgccgaccc gtcgggaacc ggcccgggga cgggatggtt ggggcggctg tgcgacagcg
     3181 agtgccccga ctgcggaccg accgcgggca tcgccatgct cggggccgag atgcccctcg
     3241 cgatgcaggg cggatcgggg aaggcggtgg tgctcgagag cccgcaggcg ttcgcgttcc
     3301 atcccatcca ggggggcggg ccgcaggagg cggaggcgtt ccgccggttg atgcagcccg
     3361 ttccgggcga ggaaccgatg gtggactttc tcacccacac ggcgatggat gcgatcctgg
     3421 cctccgacga cattcgcaag gtgacggggc ggcccggtgc ggacaccgca ccctatcccg
     3481 gcgatccgtt cagcctcaag ctgcgccttg tctccgaact gatctcggcc ggcgccccga
     3541 cgcgcgtcta ctacgtgggg ctcggggggt tcgataccca cgcggcgcag gcgggccgcc
     3601 atgaccggct gatggagcaa ctcggcgccg ggttggcggc gttcgtcggg gacctcaccc
     3661 agaagggcct gctcgagcgg gtgctggtga tgaccttctc cgagtttggt cgccgggtcg
     3721 cggagaacgc gtcgggcggc acggatcacg gggctgcggc gccgatgttc ttgatcgggt
     3781 cgcaggtggc gccgggcgtc cacggcgtgc acccgagcct cgtggatctc gatcaggggg
     3841 acctgaagtt cggggtggac ttccgaagcg tgtatgcgac cgtcctggag cagtggatgg
     3901 ggatcgggag ccaagccatc ctgggcgacc ggttccccgc cgtcggcatc ctcaggccgc
     3961 gggccggcct cttgaaagcg ggatagcgac cccgacgagc ggctgggccg gttggtctcc
     4021 cccttgcacc cggctcagcg caccggccgg gtggctttac cccggcccgg catttgcggc
     4081 ccccagcatc gcgtttcgga ccgcggtgac gagcgcttcg atccccaacg cgagcaccac
     4141 cgctttcgtc cccgccagat cgtgcccggg gggctccacg aggtagggcg gcgcgtgagg
     4201 cgggagcggc ggcagcggct ggaacgcttc ccgctcgagg gtcgatgcga ggcggctcaa
     4261 gacgtccacg cgcttctcga tctcctgcgg cgcttcgtcg gccgcgaggc cggccgggga
     4321 ggcccctgtg gggagcacgg caggacacag ggtccggact tcctcgaggc gtgtgtggat
     4381 gtcgcgcagg acctcccgga cctcgccggc gtgcgaccgg ccgaggccgg cccgcggata
     4441 ccccggccgc ggctgggtca gcggctcgtg ctgggacaac gcgcggagcc atgcggcatg
     4501 ctgctggagg gccgcgaggc tctgccataa ctggccccag cgatcctgac gggcttcggt
     4561 catcgcgggc atccctcgcg ctggcgcaat gtcggccgtc cccagcgcgc tctcgatcgg
     4621 agtctacgat cggttcgggg gaacttctcc ccaacaaccg gggagggtct cccgagcctt
     4681 tcattcgcgc tgcatgttat actccccgct gcgatgacac ggaagcacct gctgcgcacg
     4741 ctctggggct cgctgctcct ggccgcctgc gtcgtgccgg ccaatgcgca gcctgcgggg
     4801 atgcagcggg tcaccgaccc gaaaggccgc ttcaccatca gcgtcccgat cggctgggaa
     4861 gtgatgtcga tcaacaccga ccccctggcg ggcgcgttgg tgcgggacca tgccaagggc
     4921 gcattctcga tgctgatggc tgtggatccg gccgagcccc agtctccgac ggcgctcgtg
     4981 atcatgggct tcgcccttcc ccaggagatc tctccccagg cctttgggac gatgatgagc
     5041 gagccgctcc ccacccaagg tgtttgcgca gatgacggtc gtccgggaag ggaatgccac
     5101 gatcgcgggc cgaccggcat tctaccgcta catcacactg cagacgaaga gcggggacga
     5161 cctgtacgcc gtcatggtgt tctttacggt cggccaggat ggctacttga tcttcggcgt
     5221 cactcggaat gcgccggaga ccttccgcag aaacttcgcc gagatctcca ggatcctcga
     5281 gaccttccgg ccaatcggca accccggccc atcgatcgct cccggatcga agcgctctac
     5341 cgccccggct cctccggctc agcgggcggc ttgaaccgct cgaagggatt gcggcaagcc
     5401 tggcacacgt aggtggcccg gcacagcgtc gggccgaaca ggctgtccag cgcgacgtcg
     5461 cgcgatccgc aggaggggc
//
LOCUS       DASQGH010000101         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42837, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000101 DASQGH010000000
VERSION     DASQGH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42837"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..537
                     /locus_tag="VEW91_01960"
     CDS             235..537
                     /locus_tag="VEW91_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HYM90381.1"
                     /translation="MPTYEYRCTRCGHQFEAVHAVGETVARCERCGARVRRIFSPPTL
                     IFKGSGFYSTDNRKTPRPSEGDGGAPKTTKPATESKSSDSSTGTAAPKSSDAKKAS"
     gene            602..2368
                     /locus_tag="VEW91_01965"
     CDS             602..2368
                     /locus_tag="VEW91_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90382.1"
                     /translation="MLAAPGAWAGPLPGTGGVEVRLGFGGVVKIGVPVPLDVLLPPLP
                     RTGPATLVVDAPALGPEVGRVVTATSVPFQAVAGAPLTVHASVAISDPRRPLVVRVLI
                     DGQEVLRRVVPISPEQVAGRLLVALSDDRAGLASLRRLPGRVAAVTGTGSMLPRVWQE
                     YLAVDLVVIRDLDVALDPSQEEALRTWVLLGGRLLLIARPGVRIPAALEPLLPAAWGE
                     PRTVPSLAGLATSDGDALPPVPATITALMPHPGARRVTAGGLPVMASGEAGMGRVIVW
                     GIDPWLPPFLEWSGRLRWWDDALGGEGPPRIDPAALAEKLVIGTPLDPLVHMEVGGAI
                     LLYVGLLLGFLRWRPTPAGAGWSLLLVLVGVGAFALLAEATRARSATLTEATILEPID
                     GMRIARSTTIAAVAVPYGGRYRITVPRGMVAQPVTPASDLRIELSRAGTVLTGFLRSG
                     EPPRPFQAVGVAPVLASASISPDGRQLVVDLGEARAHRAELRHHDRIFPLGELPSGRS
                     VVDLQPDGWVAASADTRTLPEFSARLRDGIFQGPAGGAILGGTTPVLVAEVDRAAPVF
                     ALGGAGAPGQRLTILLVPLARR"
     gene            2382..3101
                     /locus_tag="VEW91_01970"
     CDS             2382..3101
                     /locus_tag="VEW91_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90383.1"
                     /translation="MGLVPGWGNPVLRGDLRARSGSRKIWIFQAAYLGILGTLVVLGL
                     PPELGRFAASSETSLYLAVVWVQVVLVTYCASACLAQEIAVEGEKGPVDLAFAPFSPT
                     TIVIGKSLASLVTIIFWLLLGAPLLVLTLAIRQIPLSALLPVAAFITVAAWGIAQTGL
                     LYSILCEAEFSRILVHWGTLTAVFVVTALLPPPLRELNPILAATAAAQGALPAALGAY
                     ALLGIACALWAHGRLRRLAAE"
BASE COUNT          370 a         1128 c         1218 g          524 t
ORIGIN      
        1 cggcgggcgg tggtggagcg gcgcgtccgc gacgtggtgg accgcgaact cgccgggatc
       61 catctgttct gccggaacct ggccgagggt cggtacagcg tgtgctgacc cggtctctgt
      121 acggcgttgc ttgacacgag cccggggtcg gggccacaat agggggacag atctcagccg
      181 gccccgcccg cgcggcgggc ggggctggcg tgcgtgctgg cggaggataa gcggatgccg
      241 acctacgaat accggtgcac gcgctgcggc caccagtttg aggcggtgca cgcggtgggg
      301 gagaccgtgg cccggtgcga gcgctgcggg gcgcgggtcc gccggatctt ttccccaccg
      361 acgctgatct tcaagggctc cggattttac agcaccgaca accggaagac gccccggccg
      421 tccgagggcg acggtggtgc gccgaagacg accaagcccg ccacggagtc caagtcttcg
      481 gactcgagca cgggtaccgc agcgccgaaa tcgagtgacg ccaagaaggc ctcatagccg
      541 cgacgggcgg gcgagggcat ggccgagggg ccatgccctc gctgcgcttt gtgcggtctg
      601 cgtgctcgcg gccccggggg cctgggccgg tccgcttccg gggaccggcg gcgtcgaggt
      661 gcgcctcggg ttcggcgggg ttgtcaagat cggggtcccg gtccccctgg acgtgctgct
      721 cccgccgctt ccgcggacgg gcccggccac gctcgtcgtc gacgctccgg cgctcgggcc
      781 cgaggtgggc cgcgtggtga ccgcgacctc cgtcccgttt caggcggtgg cgggcgcccc
      841 gctgaccgtg cacgcctccg tggcgatcag cgatccccgg cgcccgcttg tggttcgcgt
      901 gctcatcgat gggcaagaag tgctccgccg ggtggtgccg atctcgcccg agcaggtggc
      961 cggtcgcctg ctcgtggcgc tctcggacga tcgtgcgggg ctcgcctccc tccgccggct
     1021 gccgggacgc gtcgcggccg tcaccggcac cggcagcatg ctgccgcggg tgtggcagga
     1081 gtacctcgcg gtcgacctcg tggtgatccg cgacctcgac gtggcgctcg acccgtccca
     1141 ggaggaggcc ctgcggacgt gggtgctgct cgggggccgc ctgctgctca tcgcccggcc
     1201 cggcgtgcgc atcccggcgg ccctcgagcc gctgctcccc gcggcatggg gcgagccccg
     1261 aacggtgccc tcgctcgccg gactcgcaac gtcggacggc gatgcgctcc cgcctgtacc
     1321 ggcaacgatc acggcattga tgccgcaccc aggggcgcgc cgggtcaccg ccggcggcct
     1381 gccggtgatg gcctcggggg aggccggcat gggcagggtg atcgtctggg ggatcgatcc
     1441 gtggctgccc ccgttcctcg aatggagcgg ccgcctgcgg tggtgggacg atgccctcgg
     1501 cggcgagggt cccccgcgga tcgacccggc cgcgctcgcg gagaagctcg tgatcgggac
     1561 cccgctcgat ccgctcgtgc acatggaggt cggcggggcg atcctcctgt acgtcggact
     1621 gctcttgggg ttcctccggt ggcgcccgac acccgccggc gccgggtgga gcctgctcct
     1681 ggtcctcgtc ggcgtcgggg cgttcgcgct gttggctgag gccacccggg cccgctcggc
     1741 gacgctcacc gaggcgacca tcctcgagcc gatcgacggg atgcggatcg cgcggtccac
     1801 gaccatcgcg gcggtcgcgg tgccctacgg aggccggtac cggatcaccg tcccgcgggg
     1861 gatggtggcg cagcccgtca cgcccgccag cgatcttcgt attgaactgt cccgcgccgg
     1921 gaccgtgctg accgggttcc tgcggtccgg tgagccaccc cggccgttcc aggccgtcgg
     1981 ggtcgccccg gttctcgcgt ccgcctcgat ctcgccggac ggccgccaac tcgtggtgga
     2041 cctcggggag gcgcgggccc accgcgcgga gttgcggcac cacgatcgca tctttcccct
     2101 gggcgagctc ccatccggcc gatcggtggt cgacctccag cccgatgggt gggtggccgc
     2161 aagcgccgat acgcggacgc tgccggagtt ctccgcgcgt ctgcgcgacg gaatctttca
     2221 gggaccggcg ggcggtgcta tactgggtgg cacaacaccg gtgctggtgg cggaggtgga
     2281 ccgggccgcg cccgtgttcg cgctcggagg cgcgggagcg ccaggtcaac ggctgacgat
     2341 cctgctcgta cctctcgcgc gaaggtaggc aggaggacgc cgtggggctt gtccccggat
     2401 gggggaatcc ggtgctgcgt ggagatctcc gagcccggag cgggagtcgc aagatctgga
     2461 tcttccaggc cgcgtacctc ggcatcctcg ggaccctcgt ggtcctcggc ctcccgcctg
     2521 aactcggccg cttcgccgca tcaagcgaga cgagtctgta cctggccgtg gtgtgggtgc
     2581 aggtcgtgct cgtcacctac tgcgcgtccg cgtgtctcgc gcaggagatt gccgtcgagg
     2641 gcgaaaaggg gccggtggat ctggcgttcg ccccgttctc gcccaccacg atcgtgatcg
     2701 ggaagagcct cgcctcattg gtgacgatca tcttctggct gctgctcggc gcgccgttgc
     2761 tcgtgctcac gctcgcgatc cgacagattc ccctgagcgc gctcctcccc gtcgccgcat
     2821 tcatcaccgt cgccgcgtgg ggaatcgcgc agacagggtt gctctacagc atcctgtgcg
     2881 aggcggagtt ctcgcggatc ctcgtccatt gggggacact gacggcggtg ttcgtcgtca
     2941 cggcgttgct tccgcccccc ctccgggagc tcaacccgat cctcgccgcg acggcggcag
     3001 cccagggagc gctgccggcg gcgctcgggg cctatgcgct cctcgggatc gcctgcgcgc
     3061 tgtgggcgca cgggcgcctg cgcaggctgg ccgccgaatg acgcgcgagg cgggaatcct
     3121 tcgggccgtc ctgcgccggg tggcgctccg ggtgggcgtc ggcgaggcgg tgcggtggat
     3181 cgcccgctcg ctcgcggcgc tctcggggat cgtgctggcg tgggcgttgg tgacgatggt
//
LOCUS       DASQGH010000102         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34336, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000102 DASQGH010000000
VERSION     DASQGH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34336"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /gene="fusA"
                     /locus_tag="VEW91_01975"
     CDS             <1..1941
                     /gene="fusA"
                     /locus_tag="VEW91_01975"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYM90384.1"
                     /translation="TTDFDPEEIRRKITVNAALAPLEWQETKLNLIDTPGYPDFIGEV
                     VGALRGVESVLVAVDATAGVEVQTDKVWSLADRERLSRVVVVTRMDREHAAFPRVVER
                     VAERFGRRVVPVQWPIGAAASFNGVIDLVAMTALAPSGAVALDSLPGEVTAAANAARD
                     RLVETVAETDDALTEAYLDKGELAPDALAAGLQAGVHAGTIVPVLCVAAPRSIGVPAL
                     LEALIRWLPSPAHRTAVRARAKGGEIELKTTADGALAAQVFKTMADPYVGKLSYFRVF
                     SGTFKSDSQVWNANKAKAERVGQLFCLRGKHQEPAAEVGAGDIGAVAKLAETGTGDTL
                     TDKDRAVTLPPIEFPRPAIAMAVEPKSKADEDKLSSALARLLEEDHTLQVQRASEMKQ
                     TVISGMGESHLEIVADRLKRKFGVDVTLSVPRVPYRESVRAHARVQGRYKRQTGGRGQ
                     YGDCWIELDPKGHGEGYEFVDKIHGGAIPRNYIPSVEKGITEAMEEGMVAGYPVVDVR
                     VTLVDGSYHEVDSSDMAFKIAGSMAFKKAMQEARPVLLEPITSVAVQVPEEQMGDIIG
                     DLNSKRARIQGMEPGGDGTSIVRAQVPMAEMLRYASDLRSITGGRGTFEMEFSHYDEV
                     PAHIAERVIAEARKQKEAVESR"
     gene            2020..>2867
                     /locus_tag="VEW91_01980"
     CDS             2020..>2867
                     /locus_tag="VEW91_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM90385.1"
                     /translation="MSRLARSWALVWAIGATLLLVISIGSPERTQMALRSLSHWSAAP
                     TALAQTPESPAQLEWLGWQFFRLTSPRGKIILFNPALNDPRAAFRNRESPLNLDDITA
                     AHLILPADGHADDQGMTVEIAKKTGALVITTFELGNWMVGQGVPSEKILRSQPGFRFE
                     VEGIKIQVVNSVHGSGAPPLPGQSASVYGGPAQGYIVTLENGVRIYHAGSTALTQDLQ
                     LYGRLYKPHVALLPIAGGGQPDDAALAAEFLRTDNPNLHSVFPQHHLALPPSELAGLF
                     VHEVQSR"
BASE COUNT          476 a          942 c         1030 g          419 t
ORIGIN      
        1 acgaccgatt tcgatccgga ggagatccgc cggaagatca cggtcaatgc cgcgctcgct
       61 cccctcgagt ggcaggagac caagctcaac ctcatcgaca cgcccgggta ccccgacttc
      121 atcggcgagg tcgtcggggc cctgcgggga gtggagagcg tgctcgtcgc ggtcgacgcc
      181 accgcgggcg tcgaggtcca gaccgacaag gtctggagcc tggccgatcg agagcggctg
      241 agccgggtcg tggtggtgac ccggatggac cgggaacacg ccgcgttccc gcgggtggtg
      301 gagcgggtcg cggagcggtt cggccgccgg gtggtccccg tccagtggcc gatcggcgcg
      361 gcggcgtcgt tcaacggcgt catcgacctg gtggcgatga cggcgcttgc cccgtcgggc
      421 gcagtcgcgc tcgacagcct cccaggagag gtgaccgccg cggcgaacgc cgcccgggac
      481 cgccttgttg aaaccgtcgc ggagaccgat gacgcgctca ccgaggccta cctggacaag
      541 ggcgagttgg cgccggatgc gctcgccgcc ggtctccagg ccggcgtcca tgccgggacg
      601 atcgtgccgg tcctgtgcgt tgccgcgccc cggagcatcg gggtcccggc gctgctcgag
      661 gcgctgatcc gctggctccc ctcgccggcc caccggacgg ccgtccgggc ccgcgccaag
      721 gggggcgaga tcgagctcaa gacgaccgcg gacggcgcgc tggcggcgca ggtgttcaag
      781 accatggccg acccgtacgt cgggaagctc tcctatttcc gggtcttcag cgggaccttc
      841 aaatcggatt cgcaggtctg gaacgccaac aaggccaagg ccgagcgcgt cggccagctc
      901 ttctgcctcc ggggcaagca ccaggaaccc gcggccgagg tcggggcggg cgatatcggc
      961 gcggtggcca agctggccga aaccggcacc ggggacacgc tcacggacaa ggatcgggcc
     1021 gtcaccctgc ccccgatcga gttccccagg ccggcgatcg cgatggccgt cgagcccaag
     1081 agcaaggcgg acgaggacaa actcagctcg gcgctcgcgc gcctgctcga ggaggaccac
     1141 acactccagg tgcagcgcgc gagtgaaatg aagcagacgg tgatctccgg gatgggggag
     1201 tcccacctcg agatcgtggc cgaccgcctc aagcgcaagt tcggggtgga cgtcaccctc
     1261 tcggtcccgc gcgtgccgta tcgggagtcg gtgcgggcgc atgcgcgtgt ccaggggagg
     1321 tacaagcgcc agaccggcgg ccgcgggcag tacggggatt gctggatcga gttggacccc
     1381 aaggggcacg gcgaaggcta cgagtttgtc gacaaaatcc acggcggggc catcccccgg
     1441 aactacatcc cctcggtcga aaaggggatc acggaagcca tggaggaggg catggtcgcg
     1501 gggtatccgg tcgttgatgt ccgggtcacc ctggtcgacg gatcgtatca cgaggtcgac
     1561 tcctccgaca tggccttcaa gatcgccggt tcgatggcgt tcaagaaggc catgcaggag
     1621 gccaggccgg tcctgctgga gccgatcacg agcgtggcgg tgcaggtacc ggaagagcag
     1681 atgggggaca tcatcggcga cctcaacagc aagcgggccc ggatccaggg catggagccc
     1741 gggggcgacg ggacctcgat cgtccgggcg caggtcccga tggccgagat gcttcggtac
     1801 gcgtcggacc tccggtcgat caccggcggc cgggggacgt tcgagatgga gttctcgcac
     1861 tacgatgagg tcccggccca catcgccgag cgggtgatcg ccgaagccag gaagcaaaag
     1921 gaggcggtcg agagtcgctg agccgggccg ggctgaccgc gccggtgtga tccgaggtgt
     1981 cgggctcgtc gtgagtggtc gcattcgaga gggggaggca tgagtcggtt ggcgagatcg
     2041 tgggcgctcg tctgggcaat cggggcaacg ctgctcttgg tgatctcgat cggatcgccg
     2101 gagcgcacgc agatggcgct cagatcgctc tcccattgga gcgcggcgcc caccgccctc
     2161 gcgcaaacgc cggagtcccc ggcgcagcta gagtggctgg ggtggcagtt cttccgcctc
     2221 acgtccccgc ggggcaagat catcctgttc aaccccgcgc tcaatgatcc ccgggccgcc
     2281 ttcaggaacc gggagtcccc gctcaacctt gacgatatca cagccgcgca cctgatcctg
     2341 cccgcggatg gccacgcgga cgatcagggc atgaccgtgg agatcgccaa gaagaccggc
     2401 gcgctcgtca tcacgacgtt cgaactgggc aactggatgg tggggcaggg tgttccgtcc
     2461 gagaagatcc tccgctccca gcccggcttc cggttcgagg tcgagggcat caagattcag
     2521 gtggtgaact ccgtgcacgg gtcgggcgcg ccgccgcttc cggggcagtc ggcctcggtg
     2581 tacggggggc ccgcgcaggg gtatattgtg accctcgaga acggcgtccg gatctaccac
     2641 gccggcagca ccgcgctgac gcaggacctc cagttatacg ggcgcctcta caaaccccac
     2701 gtggccctgc taccgatcgc cgggggcggg cagcccgacg atgccgccct ggcggccgag
     2761 ttcctgcgga ccgacaaccc gaacctccac tcggtcttcc cccagcacca cctggcgctg
     2821 ccgccgagcg agctggcagg cctctttgtc catgaggtgc agagccg
//
LOCUS       DASQGH010000103         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45755, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000103 DASQGH010000000
VERSION     DASQGH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45755"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VEW91_01985"
     CDS             <1..514
                     /locus_tag="VEW91_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM90386.1"
                     /translation="HFGVHGMVDIQIGTLSKAWACLGGYVAGRRALVEYLMNRARPLL
                     FSTSHPPSVAATAIAALDLIGEEPGLIERLWENTRAFKSGLERLGYDTGMSETPITPV
                     MIGDEALAMRFSDRLFEEGVFALGIAFPTVPRGKARVRTIVTAGHTRDDLSQALEVFG
                     TVGREFGLLR"
     gene            573..1814
                     /gene="glp"
                     /locus_tag="VEW91_01990"
     CDS             573..1814
                     /gene="glp"
                     /locus_tag="VEW91_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYM90387.1"
                     /translation="MPEFLTVLTPKDAHARFAQACTPRPRGTERVPLRDAYRRVLAVD
                     IMAHEDLPEFDKSTVDGYAVRAADTAGASDGGPAQLAVVGDVQMGTPPAFGVGPGQAA
                     RIPTGGMLPSGTDAVVMVEDTEARDGVVRTRRRTAPGENVIRRAEDVRGGEVVLRRGA
                     RLRPQDLGLLAGLGITEVEVFLRPRVAVLATGDEVIPPGHRPSIGQVRDINTYTLAAL
                     IEQEGGVPQVYAIIPDDRELLLRTLEDARRSTDLVLVSGGSSVGVKDAVAWAINALGR
                     PGVIVHGVSIKPGKPTILGVVEGTPVIGLPGHPVSGMVIFDVFVRDLLRGLAGRTPPR
                     RFGRVVRARLQRRIPSAGGREDHVRVLLEERDGEVWAVPQLGKSGIITTMTRADGLVV
                     VPLGQDGVAEGAVVEVELFEP"
     gene            1867..>2267
                     /locus_tag="VEW91_01995"
     CDS             1867..>2267
                     /locus_tag="VEW91_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYM90388.1"
                     /translation="MIVPLTPLDFKRRAVRNFGDKIGVVDGERRFTYREFGERADRLA
                     GALRALDVAPGDRAAFLSYNTHHLLEAYYGVVQAGCVLTPINIRFSADDIAAVLEHAG
                     ASVLCYHRDFQGIVDRIRPRLRSLRHLILVEG"
BASE COUNT          306 a          749 c          856 g          356 t
ORIGIN      
        1 gcacttcggg gtccacggga tggtcgacat ccagatcggc acgctcagca aggcgtgggc
       61 ctgcctcggc ggctacgtgg cgggccgccg cgcgctggtg gagtacctca tgaaccgcgc
      121 ccgaccgctc ctgttcagca cctcgcatcc gccctcggtg gccgcgacgg cgatcgctgc
      181 gctcgacctg atcggggagg agccggggct gatcgagcgg ttgtgggaga acacccgcgc
      241 cttcaagtcg gggctcgagc gcctggggta cgatacgggg atgagcgaga caccgatcac
      301 cccggtcatg atcggcgacg aggcgctcgc gatgcggttc tccgatcgcc tctttgagga
      361 gggggtgttc gcgctcggga tcgcctttcc cacggtgcca cggggcaaag cccgcgtccg
      421 gacgatcgtg accgccgggc acacgcggga cgatttgtcg caggcgctcg aggtcttcgg
      481 aacggtgggg cgcgagttcg gcctccttcg ctagcgggcc gcgcggcgcc ggtccaagcg
      541 ccgagaacgc ctgtgctata cttggggatg cgatgccgga gtttcttacc gttttgacgc
      601 ccaaagacgc ccacgcccgt ttcgcccaag cctgcacgcc ccgcccgcga gggaccgaac
      661 gcgtgccgct gcgcgatgcg taccggcggg tgctggcggt cgatatcatg gcgcacgaag
      721 accttcccga gttcgacaag tcgacggtcg acgggtacgc cgtgcgcgcg gcagacacgg
      781 ccggcgcctc cgacgggggt cccgcgcagc tcgcggtcgt gggagacgtg cagatgggta
      841 cgcccccggc gtttggggtc gggccggggc aggctgcgcg gatcccgacg ggggggatgc
      901 tcccgtcggg gaccgatgcg gtggtgatgg tggaggacac cgaggcgcgg gatggggtgg
      961 tcaggacgcg gcggcggacc gcgccgggcg agaacgtcat ccgccgcgcc gaggacgtgc
     1021 ggggcgggga agtcgttctg cgacggggcg cgcggctccg cccccaagac ctgggcctcc
     1081 tggccggcct cgggatcacg gaggtcgagg tgttcctccg gccccgcgtg gcggtgctcg
     1141 ccaccggcga cgaggtcatc cctccgggcc accgcccatc gatcgggcag gtgcgggata
     1201 tcaataccta cacgctcgcc gcgctgatcg agcaggaggg gggggtcccc caggtgtacg
     1261 cgatcatccc cgacgatcgc gagctcctgc tccggaccct ggaggatgcc cgccggtcga
     1321 cagacctggt gctggtgagc ggcggcagtt cggtgggggt caaggatgcc gtggcctggg
     1381 cgatcaacgc cctggggcgt cccggggtca tcgtccacgg ggtgagcatc aagcccggga
     1441 agccgacgat cctgggggtc gtcgagggca cgccggtcat cgggctcccc gggcatcctg
     1501 tgagcgggat ggtcatcttc gatgtgttcg tccgcgatct gctccgcggc ctcgcggggc
     1561 gcaccccgcc ccgacggttc ggccgggtgg tgcgggccag gctgcagcgc cggatcccgt
     1621 ctgccggtgg gcgcgaggat cacgtccggg tcctcctcga ggagcgggat ggggaggtgt
     1681 gggcggtccc ccagttgggg aagtcgggga tcatcaccac gatgacccgc gccgacggcc
     1741 tcgtggtggt tccgctcggc caggacggcg tggcggaggg ggccgtggtg gaggtcgagc
     1801 tgttcgagcc gtagcgcgcc accgggacag acgcggcgat cctgccagtc gaaggaggaa
     1861 tggacgatga tcgttcccct gacgccgttg gatttcaagc ggcgggcggt tcggaacttc
     1921 ggcgacaaga tcggggtcgt cgacggggag cggcggttca cctatcggga gttcggcgag
     1981 cgggccgatc gtcttgcggg cgccctccgt gccctcgacg tcgcccccgg cgaccgggcg
     2041 gcgttcctct cgtacaacac ccaccacctc ctggaggcct actacggggt ggtgcaggcc
     2101 ggctgcgtgc tcaccccgat caacatccgg ttctccgccg acgacatcgc cgctgtgctg
     2161 gagcacgcgg gggcatcggt cctctgttat catcgcgact tccaggggat cgtcgaccgc
     2221 atccgcccgc gccttcgctc cctccggcac ctcatcctcg tcgaggg
//
LOCUS       DASQGH010000104         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6135, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000104 DASQGH010000000
VERSION     DASQGH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6135"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1903)
                     /locus_tag="VEW91_02000"
     CDS             complement(218..1903)
                     /locus_tag="VEW91_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYM90389.1"
                     /translation="MARARRRPGARKPRVGRRPRTGPAPDGAAPGPSLDRPFTTLSGI
                     EVARCYGPKDVAGLDDERDLGLPGAYPFTRGIYPTMYRGRLWTTRMFAGYGTAEDTNA
                     RFHYLLNQGQTGLSVAFDMPTLMGYDSDHPKALGEVGREGVAVDTVEDMDILLRGIPL
                     DRATTSMTINAPANVLLAMYIAVAASRGIGLRTIGGTIQADMLKEFIAQKEWIVPPRP
                     SMKLIQDILVYCTREVPRWNTISISGYHIREAGATAVQELAFTLADGIAYVQAGCEAG
                     LDVDAFAPRLSFFFDVHNNFFEEIAKLRAARRMWARIMRERFGAKSPRSWMLRTHAQT
                     AGVSLTAQQPMNNVVRTTLQALAAVLGGAQSLHTNSMDETYALPTEDAVTLAVRTQQI
                     IGYETGVTHTVDPLGGAYFIESLTDRMETEAGAYISKIDEMGGMLVAIERGYPMREIA
                     EASYRYQQQLERKERAIVGVTEFVSPEARPIPLLRIDPAVERRQIERLRRVRASRDQG
                     AARRALDALRRVTEQGGATMPAIVDAVTARVTLGEICDVFRAVYGEYRETAVL"
     gene            complement(1896..2894)
                     /locus_tag="VEW91_02005"
     CDS             complement(1896..2894)
                     /locus_tag="VEW91_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYM90390.1"
                     /translation="MSRDVWVLAEYAGDRPRKITYELLGKARELAQALDGEVVALALG
                     SGILGTAPELGARGADAVRVADDPLLGQYTTDAYLAVIAPILAVEQPFLLLVGSTAAG
                     RDLAPRLAARLEAGVVTDCAMVEVSDGQVEVTRPVMTRKAIARVAFRTTGLRIAVVLP
                     NVFPPPAPEPSHVAEVLPVSVALDPAEIRTRVLDVKAISRETLPLTEADIIVSGGRGL
                     RGPENFALLKDLAQVLGAAVGSSRPPVDSGWVPHDYEIGQTGKTVNPQLYIACGISGA
                     PQHLAGMSGARCIVAINKDPQAPIFSVASYGVVGDLFQILPLLTEELRRVRETTRG"
     gene            complement(2910..3695)
                     /locus_tag="VEW91_02010"
     CDS             complement(2910..3695)
                     /locus_tag="VEW91_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014371733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYM90391.1"
                     /translation="MNIVVCIKHVPDTEAPIRIRADGGGVDESGLPFVVNYYDEHGIE
                     EALRIKERLGGTVTLVTAGPARAAQALRTGLAMGADAAIHIEDPVLDDADHLGVARAL
                     AAAISAQGCDLVLCGKLATDDNAGVVGPALAEFLGFPQATAVTKLELGAGTATVHREV
                     EGAIEVLQVALPAVITVERSINEPRYPSLPGIMRAKRTPITQRTLGDLGLTLDEIGGA
                     AARIEVVGWSPPPQRQAGRVLEGEPAQVVKDLVRLLREEAKVL"
     gene            complement(3692..>4603)
                     /locus_tag="VEW91_02015"
     CDS             complement(3692..>4603)
                     /locus_tag="VEW91_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM90392.1"
                     /translation="SVNNSLDCYPIFAFGTEDQKGTYLKALARGQKLGCYCLTEPTAG
                     SDAAALQATARDAGDHWVLNGTKIFVTNGVEADVLIIYARTEAAAGSRGISAFIAEKS
                     DRGLSVGKVEHKLGIRASSTCEIVLDDCRLPKDRLLGERGKGFHIALATLDGGRIGIA
                     AQALGIARAAMEDAAGYAKERRQFGRPIAEFQATQWKIADMATRIQAGRLLTYRAAWA
                     RDQGHRHAREAAIAKLFASETAGWATSQAVQIYGGYGYIQDYPVERHFRDAKITEIYE
                     GTSEVQRLVIARHLLGPAQGGGERAQR"
BASE COUNT          658 a         1652 c         1559 g          734 t
ORIGIN      
        1 ctctatatgg gttgatcgac cagcggaacg gcatcccggt gacgcggttc aggaccgatt
       61 tgacctcgat tgggacgatc tcgcgcgggg gcatcggagc cactatagta cgaacatatg
      121 ttcgagtcaa taccgaacgc catctagctc gcctaggcgg gccgttgcgt tgctggctat
      181 tccaggcgag gggaagctgc ggtcatagcc ctgcggctca caggaccgcc gtctcgcggt
      241 actccccgta caccgcccgg aagacgtcgc agatctcgcc cagcgtcacg cgcgctgtga
      301 ccgcatccac gatcgccggc atcgtcgcgc ccccctgctc ggtcaccctg cggagcgcgt
      361 cgagcgcgcg gcgggcggcc ccctggtcgc gtgacgcgcg gacgcgcctg agccgctcga
      421 tctgccggcg ctccacggcc ggatcgatac gaagcagcgg gatggggcgc gcttccggcg
      481 agacgaactc ggtgacgccg acgatcgccc gctccttgcg ttcgagttgc tgctggtacc
      541 ggtaactcgc ttccgcgatc tcgcgcatgg ggtatccccg ctcgatcgcg accagcatcc
      601 cgcccatctc atcgatcttg ctgatgtacg cgcccgcctc cgtctccatc cggtcggtga
      661 gcgactcgat gaagtacgcc ccccccagcg gatccaccgt gtgcgtcacc ccggtctcgt
      721 aaccgatgat ctgctgcgtg cggaccgcca gcgtcacggc gtcttcggtc ggcaacgcgt
      781 aggtctcgtc catcgagttg gtgtggagcg actgcgcccc gcccagcacc gccgcgagcg
      841 cctggagcgt tgtccggacg acgttgttca tgggctgctg cgcggtcagg ctcacccccg
      901 ccgtctgcgc gtgggtccgg agcatccagg accgggggga cttggctccg aaccgctcgc
      961 gcatgattcg ggcccacatc cggcgagcgg cccggagctt ggcgatctcc tcgaagaaat
     1021 tgttgtggac atcgaagaag aacgacaggc ggggcgcgaa cgcgtcaaca tccagcccgg
     1081 cctcgcaccc ggcctgcaca taggcgatcc cgtcggcgag cgtgaacgcc agctcctgca
     1141 ccgccgtcgc gccggcctcc cggatgtggt acccgctgat gctgatcgtg ttccaccgcg
     1201 gaacctcgcg ggtgcagtac acgaggatgt cctggatcaa cttcatgctc ggccgcgggg
     1261 ggacgatcca ctccttttgg gcgatgaact ccttgagcat gtcggcttga atcgtcccgc
     1321 cgatcgtcct gagaccgatc ccccgggagg cggcgaccgc aatgtacatc gccaggagca
     1381 cgttggccgg cgcattgatc gtcatcgagg tggtggcgcg atcgagcggg atgccccgga
     1441 ggaggatatc catatcctcc acggtgtcca ccgcgacccc ttcccggccc acctcgccca
     1501 acgccttggg gtgatcggag tcgtagccca tgagcgtggg catgtcgaac gcgaccgaga
     1561 gcccggtctg gccctggttg agcaggtagt ggaaccgggc gttggtgtcc tcggcggtgc
     1621 cgtacccggc aaacatgcgc gtcgtccaga gccggccgcg gtacatcgtg gggtagatcc
     1681 ctcgggtgaa cgggtaggcc cccgggagcc ccaggtcccg ctcgtcgtcg aggccggcga
     1741 cgtccttcgg accatagcag cgggccacct cgatgcccga gagcgtcgtg aatggtcggt
     1801 cgaggctcgg cccgggcgcc gccccgtctg gagctgggcc ggtgcgcggg cgccggccga
     1861 cccggggttt gcgcgcgccg ggccgccgtc tggccctagc cacgggtggt ctcccggacc
     1921 ctgcggagtt cctcggtgag gagggggagg atctggaaga ggtcgcccac caccccgtac
     1981 gaggccaccg aaaaaatcgg cgcctggggg tccttgttga tggccacgat gcagcgcgcg
     2041 cccgacattc cggcgaggtg ctggggcgcc cccgagatgc cgcaggcgat gtacagctgc
     2101 gggttcaccg tcttgccggt ctggccgatc tcgtagtcgt gggggaccca cccggagtcc
     2161 accgggggcc ggctcgatcc caccgccgcc cccaggacct gggcgaggtc cttgaggagc
     2221 gcaaagttct cgggcccccg aagcccccgg cccccggaca cgatgatatc ggcctcggtc
     2281 agcggcagcg tctccctgga aatggccttc acgtccagca cccgggtgcg gatctcggcc
     2341 ggatcgagcg cgaccgacac cggaagcacc tcggccacgt gcgacggctc cggggccggc
     2401 ggcgggaaga cgttggggag gaccacggcg atgcgcaatc cggtcgtgcg gaacgccacg
     2461 cgcgcaatcg ccttccgggt catcacgggt ctcgtcactt cgacctggcc gtcgctgacc
     2521 tcgaccatcg cgcagtcggt gacgacgccg gcctccagcc gcgcggcgag ccgcggggcg
     2581 aggtcgcgcc cggcggcggt gcttccgacg agcagcagaa acggctgctc caccgcgagg
     2641 atcggcgcga tcaccgcgag gtacgcatcg gtcgtgtact ggccgaggag ggggtcgtcg
     2701 gccacccgca cggcgtcggc gccgcgggcg ccgagctcgg gtgccgtgcc cagaatcccg
     2761 gacccgagcg ccaaggccac cacctcgccg tcgagcgcct gggccagttc ccgggctttg
     2821 ccgagcaact cgtaggtgat cttgcggggg cggtcacccg cgtactcggc caggacccag
     2881 acgtcacggc tcatgcgcgc gccccggggt cagaggacct tggcctcttc acggaggagc
     2941 cgtaccaggt ccttgaccac ctgggccggc tcgccctcga ggacccgccc cgcctgccgc
     3001 tgggggggcg ggctccaccc gaccacctcg atccgggcgg cggcgccccc gatctcgtcc
     3061 agcgtcaacc cgagatcccc cagcgtcctc tgggtgatcg gcgtccgctt cgcccgcatg
     3121 atgccgggga gggacgggta ccgcggttcg ttgatgctcc gctcgaccgt gatcaccgcc
     3181 gggagcgcca cctgcaggac ctcgatcgcg ccctcgacct cccggtgcac cgtcgccgtc
     3241 cccgccccca gctccagctt cgtgaccgcg gtggcctggg gaaatcccag gaactccgca
     3301 agggccgggc cgaccacccc cgcgttgtca tcggtggcga gcttcccgca gagcaccaga
     3361 tcgcagccct gggccgagat cgcggccgcg agcgcccggg ccacgccgag gtggtccgcg
     3421 tcatccagca ccggatcctc gatgtggatg gccgcgtcgg cccccatcgc cagcccggtc
     3481 cggagcgcct gggccgcccg ggcggggccg gcggtgacca gcgtcaccgt tccgccgaga
     3541 cgctctttga tgcggagggc ctcctcgatg ccgtgctcgt catagtagtt gaccacaaac
     3601 ggcagcccgc tctcgtcgac gccgcctccg tctgcgcgaa tccggatcgg cgcctcggta
     3661 tcgggaacgt gcttgatgca gacgacgatg ttcaacgctg ggctcgctct ccaccgccct
     3721 gggccggccc gaggaggtgg cgggcgatca ccagccgctg gacctcggac gtgccttcgt
     3781 agatctcggt gatcttggcg tcacgaaagt ggcgctcgac cgggtaatcc tggatatacc
     3841 cgtacccgcc gtagatctgg accgcctggg aggtggccca ccccgccgtt tcggacgcga
     3901 acagcttcgc gatcgcagct tcgcgggcgt gccggtgccc ctggtcgcgc gcccaggccg
     3961 cgcggtaggt gagcagccgc ccggcctgga tcctcgtcgc catatcggcg atcttccact
     4021 gggtcgcctg gaactccgcg atcggccgcc caaactggcg gcgctccttg gcgtacccgg
     4081 ccgcgtcctc catcgccgcg cgcgcgatcc ccagggcctg ggcggcgatc ccgatccggc
     4141 cgccgtcgag cgtggcgagg gcgatgtgaa accctttccc gcgctccccg agcagccggt
     4201 ccttgggcag ccggcagtca tccagcacga tctcgcaggt gctgctcgcc cggatgccga
     4261 gtttgtgctc gaccttcccg accgagagcc cccggtcgct cttctccgcg atgaacgcgc
     4321 tgatgccccg gctgccggcg gcggcctccg tccgggcgta gatgatcagg acgtccgcct
     4381 cgacgccgtt cgtcacaaag atcttcgtcc cgttgaggac ccagtgatcg cccgcgtccc
     4441 gggcggtggc ctgcagggcg gccgcatccg atcccgcggt cggctcggtg aggcagtagc
     4501 acccgagctt ctgcccacgc gccagggcct tgaggtacgt gcccttctga tcttcggtgc
     4561 cgaaagcgaa gatcggatag cagtcgagcg agttgttgac gga
//
LOCUS       DASQGH010000105         7379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48545, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000105 DASQGH010000000
VERSION     DASQGH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48545"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..907)
                     /gene="trpC"
                     /locus_tag="VEW91_02020"
     CDS             complement(122..907)
                     /gene="trpC"
                     /locus_tag="VEW91_02020"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYM90393.1"
                     /translation="MAGVLREIVAHKAQELAGRRQRIPLDQLRGRAAAADPPRPFLAA
                     IRGPRIRLIAEVKGASPSAGTIRTDFDPAAIARGYEAAGAAAISVLTDARYFRGADAH
                     LAEVRRAVGVPVLRKDFVIDPYQVYEARALGADAVLLIVAILSPTALGDLQRLAHELG
                     MAALVEVHTEADLEGALAARAPLIGINNRNLDTLEVALDPSRRLRPRIPDGVTVVAES
                     GIEGRAQVEEMEQLGMHAVLVGTALMRAPDPAARVRELLGIAR"
     gene            complement(918..1934)
                     /gene="trpD"
                     /locus_tag="VEW91_02025"
     CDS             complement(918..1934)
                     /gene="trpD"
                     /locus_tag="VEW91_02025"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYM90394.1"
                     /translation="MPLRPAIAKVVAHQTLTGAEAHQAMGHIMEGDATPAQIASFITA
                     LAMRGETVEEITGFARAIREHAITIAPKADHLVDIVGTGGDRLNTFNVSTTSAFVIAG
                     AGGHVAKHGNRAASSRCGSADVLEALGINLQAAPEVVQACIEEVGFGFMFAPHFHPAF
                     KHALSPRREIGIRTVFNILGPLTNPARARRYLQGAPSAAHTELMARVLAALGADRAFV
                     VHGQDGMDEISISAPTQVSEVADETVRTYTVSPEDLGFTRAPLEALRGGSPEENAEIC
                     VSILRGERGPRRDIVLLNAGAARVAAGLAGKIPDGAAQAARSIDSGAAYATLEALRLK
                     TKFA"
     gene            complement(1937..2542)
                     /locus_tag="VEW91_02030"
     CDS             complement(1937..2542)
                     /locus_tag="VEW91_02030"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HYM90395.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGEDPRVFRNDAITVDKIVALAP
                     DAIVISPGPCTPAEAGVSAPSIAALAGRIPILGVCLGHQCIGAALGAPVVRGRAPVHG
                     KTSRIHHDGRTIFTGLPSPLIGTRYHSLVIDEAGLPECLEVSARTEDRVVMGVRHRTA
                     LVEGVQFHPESVLTEHGRAILRNFLDLARGKALVPAAAREG"
     gene            complement(2559..4040)
                     /gene="trpE"
                     /locus_tag="VEW91_02035"
     CDS             complement(2559..4040)
                     /gene="trpE"
                     /locus_tag="VEW91_02035"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYM90396.1"
                     /translation="MAKALTLEPGTREFDRRAREGNLVPVSCEVVADLDTPISAYLRL
                     RDLPQPFLLESVEGGEKVARYSFLGAQPRLTLRAFPTHVEVVEGGATRRSGQDPLEVA
                     RAILQQYRPAADPSLPRFYGGLVGWFGYDLVRTIEHLPNRPPDDRGLPLCSLQLADAV
                     MIFDHLRHRIRIVANAFVEEGAERAYRGAQRRIEEWLDRLRRPLPEPAESGPPSPLAV
                     RSNMTREQYLAAVRRCKEYIFAGDAVQIVLSQRFAGDVGALDPFAVYRALRMINPSPF
                     MFFLEGGEAALVGASPELLVRLEGDLVEMRPIAGTRRRGLTEDEDRQLAEELLADEKE
                     RAEHVMLVDLTRNDIGRIARYGTVRVSELMAVERYSHVMHIVSSVQGRLAPGRDALDV
                     LRAVFPHGTVSGAPKVRAMEILDELEPTARGPYAGAVGYVGFGGALDTCIGIRTLVIM
                     HGTAYAQAGGGIVADSDPSAEYDETINKAKVLIRAIEAAGRGL"
     gene            complement(4043..4909)
                     /locus_tag="VEW91_02040"
     CDS             complement(4043..4909)
                     /locus_tag="VEW91_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HYM90397.1"
                     /translation="MEPFPRVSIVGVGLIGGSLGMALRARGLAREVVGVTRVEARIAQ
                     ARARGAIDRGTVDPASGVDGADLVVLAVPPDQVVPMARRVLPHLRPGALLTDVASVKA
                     EIVRSVDALLRPETQVTFVGGHPMAGNEGQGIAAAEPGLFEGTAYLITPTPRTPRAGV
                     TRVADLARAVGAVPIEMPPDEHDRAVALTSHLPYLVAAALMGASGAAIPAAGPAFLGA
                     TRVAGSPVALWAQICRLNREQIRRALGAFRDELARLEAAIEDGDRLDALLEAARRARR
                     RLGATPHDPGAG"
     gene            complement(4918..5940)
                     /gene="aroF"
                     /locus_tag="VEW91_02045"
     CDS             complement(4918..5940)
                     /gene="aroF"
                     /locus_tag="VEW91_02045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HYM90398.1"
                     /translation="MIVVMGPGHTRTQVDAVVAKIEEAGLRGQISEGVERIVIGVVGD
                     SHTKELLRDRLEAMPGVESVVRILQPYKLVSREFHGGIDTTVWVGPVAIGGGRVVVIA
                     GPCSVESREQVLETAAAVKAAGAQLLRGGAFKPRTSPYSFQGLEEDALKILAEARELT
                     GLPVVTEAMDARQLELVVRYADMVQIGARNMQNYTLLRDAGRAHHPVMVKRGPSATIE
                     ELLLAAEYVMAEGNPNVVLCERGIRTFEQYTRFTLDLNAVPVLKRLTHLPVLVDPCHG
                     TGKWELVTPMARAAVAAGADGLLVEVHPDPAKALSDGPQQLTPANFLAMMQELDAVAL
                     AVGRRV"
     gene            complement(6306..7046)
                     /locus_tag="VEW91_02050"
     CDS             complement(6306..7046)
                     /locus_tag="VEW91_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYM90399.1"
                     /translation="MTLRLDQVLAASVVVCVASMVKGGIGFGFPLLAVPLLSTIMGPR
                     VAIPVIAIPTLLSNVLVVRRGGAGRAAGVVWMALAGLAAGTVAGALLLGSLDPGRLSA
                     LVGAVAVLYVLATALHLTLRIPTTGVRLAGPVFGVAAGLLGGSTGISSPLLASYLHLL
                     RLEKREFVFWLTMMFFVVNICQVVVYARLGLYSGPVLAIALWACAPMAIGTLAGLALQ
                     DRLDPRVFERIVLAVVFAASLNLLARSL"
     gene            complement(7043..>7379)
                     /locus_tag="VEW91_02055"
     CDS             complement(7043..>7379)
                     /locus_tag="VEW91_02055"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543822.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYM90400.1"
                     /translation="VRAVAACLAEGETVIRDAAELRVKESDRVEALARELGRLGAAIE
                     PQPDGLAIRGGQRLRGGRVASGGDHRIAMALAVAGLCADGPVTIDDPACIETSFPGFA
                     QALREATGG"
BASE COUNT         1087 a         2775 c         2538 g          979 t
ORIGIN      
        1 tcgggcgatc cccggtgagg aggcgcccct tcttgcgccc gccggggcgc gcctcgggcc
       61 tgaccttcgt ctgcgcgact gccataatcc ctcctgccaa tggcggccgg ccgcggccgc
      121 gttatcgcgc gatgccgagc agttcccgca cgcgggccgc cgggtccggg gcccgcatca
      181 gcgccgtgcc cacgaggacc gcgtgcatgc ccaactgctc catctcctcg acctgcgcgc
      241 ggccctcgat cccgctctcg gcgaccaccg tgacgccgtc cgggatccgg ggccgcaacc
      301 tcctcgaggg gtcgagcgcc acctcgaggg tgtccaggtt gcggttgttg atgccgatga
      361 gtggggcccg ggcggcgagc gccccctcga gatccgcctc cgtgtgcact tcgacgagcg
      421 cggccatccc gagctcgtgc gcgagcctct gcaggtcccc gagggccgtc ggcgagagga
      481 tggcgacgat caggagcacc gcatccgccc ccaacgcccg cgcctcgtac acttgatagg
      541 ggtcgatcac gaaatccttt cggaggacgg gcaccccgac cgcccgccga acctcggcga
      601 gatgcgcatc cgcgccccgg aagtaccggg cgtcggtgag gaccgagatc gcggcggccc
      661 ccgctgcctc gtacccccgc gcgatcgcgg cgggatcaaa gtccgtccgg atcgtccccg
      721 ccgagggcga ggcgcccttc acctccgcga tcagccggat gcgcggcccg cggatcgcgg
      781 caagaaacgg ccgcggggga tccgccgccg cggcccggcc gcggagctga tcgagcggga
      841 tccgctgccg gcggccggcg agctcttggg ccttgtgcgc aacgatctca cggagcacgc
      901 cagccacgcc ccgcgcctca cgcaaacttg gtcttgaggc gaagcgcctc aagcgtggcg
      961 taggcggcgc ccgagtcgat gctccgggcg gcctgggcgg ccccatcggg gatcttcccg
     1021 gccagccccg cggcgacccg cgcggcgccg gcgttcagga gcacgatgtc gcggcgcggc
     1081 ccgcgctccc cccgcaggat gctgacgcag atctccgcgt tctcctccgg gctgcccccg
     1141 cggagcgcct cgagcggggc gcgcgtgaat cccaggtcct cggggctgac ggtataggtg
     1201 cgcacggtct cgtccgccac ctcggagacc tgcgtcggcg cggagatgga gatctcgtcc
     1261 atgccgtcct gcccgtggac gacaaacgcc cggtcggcgc cgagcgccgc cagcacccgc
     1321 gccatcagct ccgtgtgcgc cgcgctgggg gcgccctgca gataccgccg ggcgcgcgcc
     1381 gggttggtca gcgggccgag gatgttgaac accgtccgga tgccgatctc gcgccgcggg
     1441 ctcagcgcgt gcttgaacgc cggatgaaag tggggggcga acatgaaccc gaaccccacc
     1501 tcctcgatgc acgcctggac cacctcgggg gctgcctgga ggttgatgcc gagggcctcg
     1561 agcacgtcgg cgctcccgca ccgggagctc gcggcccggt tgccgtgctt ggcgacgtgg
     1621 cccccggccc ccgcgatcac gaacgccgag gtggtggaga cgttgaaggt gttcaaccgg
     1681 tccccgccgg tcccgacgat gtcgaccagg tgatccgcct tcggggcgat cgtgatggcg
     1741 tgctcgcgga tggcgcgggc gaacccggtg atctcctcga ccgtctcccc gcgcatcgcc
     1801 agcgcggtga tgaaggaggc gatctgcgcc ggggtggcgt cgccctccat gatgtggccc
     1861 atcgcctggt gggcctccgc cccggtcagc gtctggtggg cgaccacctt tgcgatcgcg
     1921 ggtctgagcg gcatccctac ccctcccggg cggcggccgg gaccagggcc ttcccccgcg
     1981 ccagatctag gaagtttctc aagatggcgc gcccgtgctc ggtgaggacc gattcggggt
     2041 ggaactggac gccctccacc agcgcggtgc ggtgacgcac ccccatgacg acgcgatcct
     2101 cggtgcgcgc cgagacctcc aggcactccg gcaggccggc ttcatcgatc acgagcgagt
     2161 gatagcgcgt gccgatcagc gggctgggca gcccggtgaa gatcgtccgg ccatcgtggt
     2221 ggatccggga agttttcccg tgcaccggcg cgcgaccccg caccaccggg gcgccgagcg
     2281 cggcgccgat gcactggtgg cccaggcaga cccccaggat cgggatccgg ccggcgaggg
     2341 ccgcgatgct cggggcgctg acgccggcct cggcgggcgt gcacggcccg ggggagatca
     2401 cgatcgcgtc cggtgcgagg gcaacgatct tgtccaccgt gatggcgtcg ttccggaaca
     2461 cccgcgggtc ctcgccgagt tccccgaggt actgcacgag gttgtaggtg aacgagtcgt
     2521 agttgtcgat caccaggatc atcgtcgacc tccagctctc agagcccgcg ccccgccgcc
     2581 tcgatcgcgc ggatcagcac cttggctttg ttgatcgtct cgtcgtactc cgccgatggg
     2641 tcggagtcgg ccacgatccc gcccccggcc tgggcgtacg ccgtgccgtg catgatcacc
     2701 agggtccgga tcccgatgca ggtgtcgagc gcgcccccga accccacgta cccgacggcg
     2761 ccggcgtagg gaccgcgggc ggtgggctcg agctcgtcga ggatctccat cgccctcacc
     2821 ttgggggccc cgctcacggt gccgtgggga aacaccgcgc gcaggacgtc gagggcgtcg
     2881 cgccccggcg cgagccgccc ctggacgctg ctgacgatgt gcatgacgtg cgagtaccgc
     2941 tcgaccgcca tcagttccga cacccggacc gtgccgtacc gggcgatccg cccgatatcg
     3001 ttccgggtca ggtcgaccag catgacgtgc tccgcccgct ccttttcatc ggccagcagc
     3061 tcctcggcca gctgccggtc ctcgtcctcg gtgagcccgc gccgccgcgt cccggcgatc
     3121 ggccgcatct cgaccaggtc cccctcgagg cggaccagca actcgggcga tgcccccacg
     3181 agcgcggcct ccccgccctc caggaagaac atgaacggcg agggattgat catccggagc
     3241 gcccggtaca ccgcgaacgg gtcgagcgcg ccgacgtcgc cggcgaaccg ctgcgagagg
     3301 acgatctgga ccgcgtcgcc ggcaaagatg tactccttac agcgccggac ggccgccagg
     3361 tactgctcgc gggtcatgtt ggagcggacc gcgagggggg agggcgggcc cgattcggcc
     3421 ggctcgggca ggggccgccg gagacggtcg agccactctt cgatccgcct ctgcgcgccg
     3481 cggtaggcgc gctccgcccc ttcttccacg aaggcgttcg ccacgatccg gatccggtgg
     3541 cggaggtggt cgaagatcat cacggcatcc gccagttgga ggctgcacag cggcaggccg
     3601 cggtcgtcgg gaggccggtt cggcaggtgc tcgatcgtcc tgacgaggtc gtacccgaac
     3661 cagccgacga ggcctccgta gaaccggggc agcgagggat cggcggccgg ccggtactgc
     3721 tggagaatgg cccgcgcgac ctccagcgga tcctgcccgc tgcgccgggt ggcgccgcct
     3781 tcgaccacct ccacgtgcgt ggggaacgcc ctgagggtca gccgcggctg ggccccgagg
     3841 aacgaatagc gcgcgacctt ctctccgcct tccacgctct cgagcaggaa gggctgcggg
     3901 agatcgcgga ggcggaggta cgcggagatc ggggtgtcga ggtccgcgac gacctcgcac
     3961 gagaccggca cgaggttgcc ctcgcgcgcc cggcggtcga actcccgggt cccgggttcc
     4021 agcgtgagcg ccttggccat ccctaccccg ctcccggatc gtgcggcgtc gcgccgagcc
     4081 ggcgccgggc ccgccgggcg gcttcgagga gcgcatccag ccggtcccca tcctcgatcg
     4141 cggcctcgag ccgggccagc tcatcccgaa aggcgccgag cgcgcgtcgg atctgctccc
     4201 ggttgagccg gcagatctgc gcccacagcg ccaccgggct gcccgcgacc cgcgtagctc
     4261 ccagaaacgc cgggcccgcc gcggggatgg cggcgccact cgcccccatc agggcggccg
     4321 ccacgagata cgggaggtgg ctcgtgagcg cgaccgcacg gtcgtgctcg tccgggggca
     4381 tctcgatcgg gaccgcgccg accgcgcgcg ccagatcggc cacccgggtg acgcccgcgc
     4441 gcggagtccg cggcgtgggc gtgatgaggt acgcggtccc ctcgaacaat cccggctcgg
     4501 cggcggcgat gccctgaccc tcgttgcccg ccatcgggtg gcctcccacg aacgtcacct
     4561 gggtctcggg cctgagcagc gcgtccaccg accgcacgat ctcggccttc acgctcgcga
     4621 cgtcggtcag cagcgctccc ggccggaggt gggggagcac ccggcgtgcc atcgggacca
     4681 cctgatccgg agggacggcc aggaccacga ggtcggcccc atctacgccg gaagcgggat
     4741 ccacggtccc ccggtcgatg gccccccgcg cgcgggcctg agcgatccgc gcctcgaccc
     4801 gggtgacacc cacgacctca cgggccagac cccgcgcccg cagcgccatc ccgagggagc
     4861 cgccgatcag gcccaccccg acgatcgaga cccgcggaaa gggctccacg cgacgactca
     4921 cacgcggcgc ccgaccgcca aggcgaccgc atcgagctcc tgcatcatgg ccaggaaatt
     4981 cgccggcgtg agctgctgcg gcccgtcgct gagcgctttg gcaggatcag gatgcacctc
     5041 gaccagcagc ccatcggccc cggcggccac cgccgcccgc gccatcggcg tgacgagctc
     5101 ccacttgccg gtgccgtggc aggggtcgac gaggaccggc aggtgggtca ggcgcttcag
     5161 caccgggacc gcgttgaggt cgagcgtaaa ccgggtgtac tgctcgaacg tccggatgcc
     5221 gcgctcgcag agcaccacgt tcgggttccc ctcggccatg acgtattcgg cggccagcag
     5281 caactcctca atcgtggcgc tcggcccccg cttgaccatc acgggatggt gcgcccgacc
     5341 ggcatcgcgc aggagggtgt agttctgcat gttccgggcc ccgatctgca ccatatccgc
     5401 gtaccggacg accagctcca gttgccgggc gtccatggcc tcggtgacga ccggcagccc
     5461 cgtaagctcg cgggcctcgg ccaggatctt gagggcgtcc tcttcgagcc cctgaaacga
     5521 gtacggcgag gtgcgcggct tgaacgcgcc gccgcgcagc agttgggcgc cggccgcctt
     5581 caccgcggcg gcggtctcga gcacctgctc ccggctctcg accgagcagg ggccggcgat
     5641 cacgaccacc cggccgcccc cgatcgcgac gggcccgacc cacacggtcg tgtcgatgcc
     5701 accgtggaac tcgcggctga cgagcttgta cggctgcagg atgcggacga cgctctcgac
     5761 gccgggcatc gcttcaaggc gatctcggag cagttccttc gtatggctgt cgccgacgac
     5821 cccgatcacg atccgctcaa ccccctcgga gatctgccca cggaggcccg cctcctcgat
     5881 cttggcgacg accgcatcga cctgcgtgcg agtatgtcca ggccccatga ccacgatcat
     5941 tgcacagcct cctcaccgcc cccaaaaaca caacaacctc ccgtcccgag ggacgggagg
     6001 cctgagacac cacccgcggt accaccccac ttccccggcc acacacgccg gggcgctcgc
     6061 cggcgaccag gcaccccatc gacccgcctc gtcgcccgcc tccggttacg gcggagacag
     6121 ccggccacgg ctactgggca gcgcagccgc cgttcgcccg cccctcaggg acccattccg
     6181 ctccggcgtc ccccgcgctc ccaccgatca cgcgggtcgc taatggaccg ggccgggcgt
     6241 actcgctccc gtcatcgggt ttggtacccc ccactatacc acacggcgtc aagggctgac
     6301 ggctcctaca ggctcctcgc cagcaggttg agggatgccg cgaagaccac ggcgaggacg
     6361 atccgctcaa acaccctggg gtcgagccgg tcctgcagcg ccagccccgc cagggtcccg
     6421 atcgccatcg gcgcacaggc ccagagcgcg atcgccagga ccggtcccga gtagaggccc
     6481 agccgggcgt agaccacgac ctgacaaatg ttgacgacga agaacatcat ggtgagccag
     6541 aagacgaact cgcgcttttc caggcggagg aggtgcaggt agctggcaag gagcggggac
     6601 gagatcccgg tcgacccgcc caggaggccc gccgccaccc cgaacacagg cccggccagc
     6661 cggaccccgg tggtagggat cctgagcgtg agatgcagcg cggtggcgag cacgtacagg
     6721 acggccaccg ccccgacgag cgcggagagc cgcccgggat cgagcgatcc caggaggagg
     6781 gcgcccgcca cagtgcccgc ggcaaggccg gcgagcgcca tccagacgac gccggcggct
     6841 cgtcccgcac cgccgcggcg gaccacgagc acattgctca gcaacgtggg aatggcgatg
     6901 accggaatcg cgacgcgcgg ccccatgatc gtcgacaaca gcggcaccgc gaggagcggg
     6961 aatccgaacc cgatcccccc cttgaccatc gaggcgacgc agaccaccac cgatgcagcg
     7021 agcacctggt cgaggcggag cgtcatccgc cggtggcttc ccgaagggcc tgggcgaacc
     7081 cgggaaagga cgtctcgatg caggccggat cgtcgatcgt cacggggcca tcggcgcaca
     7141 gcccggcgac ggccagggcc atggcgatcc ggtggtcgcc gccgcttgcg acccggccgc
     7201 cccgcagccg ctggccgccc ctgatcgcca gcccgtccgg ctgcggctcg atggcggcgc
     7261 ccaaccgtcc caactcgcgg gcgagcgcct ccacgcggtc ggactccttg acccgaagct
     7321 cggcggcgtc gcggatcacc gtctcgccct cggccaggca cgcggccacg gcccgcacg
//
LOCUS       DASQGH010000106         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6137, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000106 DASQGH010000000
VERSION     DASQGH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6137"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VEW91_02060"
     CDS             complement(<1..1173)
                     /locus_tag="VEW91_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HYM90401.1"
                     /translation="MIRLALLLMLPVALMAPAAPEPAGSPAGPGQRAPAYTIRAVLLT
                     GEPAPSGGTFTGFSDPALNDRGDLAFAALTSSPGAHAALYLRAGGRISTLAVAGRPAP
                     TGGTFTVFNDVVLSGRDTVVFLGRTTDPAAHTGLYLARAGTISAVVATGQPTPAGGVF
                     TDFANPTINGRDVVAFVGRMSQGAAEGIFTSSEGSVTSAVLTGQPAPTGGVFQFFLDG
                     SPAQNDRGEIAFIASTTPRGVFGVYVLTGGRPVPVVTTDEEAPVGGAFTEFGSLMLTA
                     AGTVGFIGRTALSAVREALYVTGRAVLVPLAQQGQAVGGEALTTFANAAMNDDEAMVF
                     QLGTPDPIPRAIFLATRTGVRVVVGAGDVARSGRRFTAFGTPTINSRGQVGFVAETD"
     gene            1286..2194
                     /locus_tag="VEW91_02065"
     CDS             1286..2194
                     /locus_tag="VEW91_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM90402.1"
                     /translation="MPTIVDSHLHLVTAGMFRRLRNRDWGLRPRALAALTGEGGRWAE
                     RLRRLEGVTLAQQAAAWADAFDRAGVDTGFFIAVGEGNEELAEFARLNPARFQAWGSV
                     LDPAAPPAAATVGRFREWGLRGLKLYPPLQRFSASDRAVYPVYEAAAAQGLPVLLHFG
                     ITVAPIYDLRYADPLHLSAAAREFPEITFVIAHCGAGFLREALLLAYHTENIWVDTSG
                     TNNWREFIPGAPHLEDVFRDLLRTYGPRRIMFGTDSTVPEEYRQGILAEQRGVFERLT
                     PTDEDRRAIFGGNARRLFRLPGGQGA"
     regulatory      complement(2402..2480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2610..3101
                     /locus_tag="VEW91_02070"
     CDS             2610..3101
                     /locus_tag="VEW91_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90403.1"
                     /translation="MSPSHSVGYARALLAGGAAGVVASVVCLGVRVVLPKPGPPPEAV
                     AGSAFVAGVLGGLVFGWWDRVVRRPGLALWITSVALATLTSAFVAMLPAPATRISSLF
                     YPVAGLVFYLEQAAALVGIGRFASFHVSGSYILADTVLHYVTAIAVSLLVPLWARPAP
                     RAP"
     gene            complement(3282..>3599)
                     /locus_tag="VEW91_02075"
     CDS             complement(3282..>3599)
                     /locus_tag="VEW91_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HYM90404.1"
                     /translation="LIVISMASAWGIVESLGIRGDVWFKIYVVETLPAVIIPLLFGHL
                     IALILGLMVALVFVLIGPVIVLGILAQKKQLMGRHVLSKFDKIAFWVSVLVVIGCGVL
                     AFL"
BASE COUNT          579 a         1223 c         1202 g          595 t
ORIGIN      
        1 gtcggtttcg gcgacgaagc cgacctgccc ccggctattg atcgtggggg tcccgaacgc
       61 ggtgaaccgc cgtccggagc gggccacatc ccccgccccc acgaccaccc gcacgccggt
      121 ccgggtggcg aggaagatgg cgcggggaat gggatcgggg gttcccagtt gaaacaccat
      181 cgcctcgtcg tcgttcatcg cggcgttggc gaacgtcgtc agggcctccc cgcccaccgc
      241 ctgcccctgc tgggccaggg ggacgaggac ggcgcgcccg gtgacgtaga gggcctcgcg
      301 gacggcgctg agggcggtcc ggccgatgaa tcccacggtc ccggcggcgg tcagcatcag
      361 cgatccaaac tcggtgaacg ccccccccac cggcgcctcc tcgtcggtgg tgacgaccgg
      421 aacgggccgg ccccccgtca ggacgtacac cccaaagacc ccccgcgggg tggtcgaggc
      481 gatgaaggcg atctcgcccc ggtcgttttg cgcagggctg ccgtccagaa agaactggaa
      541 cacgcccccg gtgggggccg gctggccggt caggaccgcg gacgtcacgc tcccctcgct
      601 gctggtgaag atgccttcgg ccgcgccctg gctcatgcgc ccgacaaacg ccaccacgtc
      661 cctgccgttg atcgtcgggt tggcgaagtc ggtaaacacc cccccggcgg gggtgggctg
      721 gcccgtcgcg acgaccgcac tgatcgtccc ggcccgcgcg aggtacagcc ccgtgtgcgc
      781 cgcgggatcg gtggtccgcc cgaggaacac caccgtgtcc cggccgctca ggaccacgtc
      841 gttgaagacc gtgaacgtcc ccccggtcgg ggcggggcgc ccggcgaccg caagcgtgct
      901 gatccggccc cctgcccgca ggtacagcgc ggcgtgcgcc cccggggaac tcgtcagcgc
      961 ggcgaacgcc aggtcgccgc gatcgttcaa cgccggatcg gagaatcccg tgaacgttcc
     1021 tccgctgggc gcgggctccc cggtcagcag gaccgcgcgg atcgtgtacg ccggagcccg
     1081 ctggcccggc cccgccgggg atccggcggg ttcgggcgcc gcgggggcca tgagcgccac
     1141 agggagcatc agaaggaggg ccagccgtat catccttccc taatcctaat ccgaagggga
     1201 acaactgcac aacggggtcc gaggggcttt caggcgcgcg gccgcgaatc tcctcccggc
     1261 ggcgttctcg aaaggagacg gtcccgtgcc gacgatcgtc gacagccacc tgcatctcgt
     1321 caccgcgggg atgttccggc gcctcaggaa ccgcgattgg gggctccggc cgcgggcgtt
     1381 ggcggccctc accggggaag ggggccggtg ggccgagcgg ctgcggcgcc tggagggggt
     1441 gacgctggca cagcaggccg ccgcgtgggc cgacgcgttc gaccgcgccg gtgtggacac
     1501 cgggtttttc atcgccgtag gcgaggggaa cgaggaactg gccgagttcg cgcgcctcaa
     1561 cccggcgcgg ttccaggcgt ggggcagcgt cctggatccc gccgcgcccc cagcggcggc
     1621 gaccgtcggc cggtttcggg agtggggcct gcgcggcctc aagctctatc cccccctgca
     1681 gcggttcagc gcgagcgacc gggccgtgta tcccgtgtac gaagccgcgg cggcccaggg
     1741 cctccccgtc ctcctccact tcggcatcac ggtcgctccc atctacgatc tgcggtacgc
     1801 ggacccgctc cacctttcgg ccgccgccag ggagttcccg gagatcacct ttgtcatcgc
     1861 gcactgcggc gcggggttcc tgcgggaggc gctgctcctc gcctaccaca ccgagaacat
     1921 ctgggtggac acgtcgggga ccaacaactg gcgggagttc atcccggggg ccccacacct
     1981 ggaggacgtg ttccgcgacc tgctccggac ctatggtccc cgccggatca tgtttgggac
     2041 cgactccacg gttcccgagg agtaccggca ggggatcctc gccgaacagc gcggggtatt
     2101 cgagcggctc accccgacgg acgaggaccg acgcgcgatc ttcgggggga acgcccgccg
     2161 gctgttccgg ctgccggggg gccagggcgc ctagccgtcg ggggcccaga caaacggaca
     2221 aggccgggga ggggtgcccg gcctcgtcgc cttagggggg gaggtgcact agctcttagg
     2281 tcagtggtcc tccggcaaag cggcagcccg gccggcgggg gcggagacga aatccaatac
     2341 ggggatggga tcgcaacctg tcgtggcgtc aaaacaagaa cgccccatca ccgatctccc
     2401 cttcggcaac ccggctgacc tcgatcggag gtcccgtggc tttgcgtccc tgggtcgccc
     2461 caggtttgcc tttgtcggta ggagacccgg atgcggcgtg catcgctccg gtgctcctcc
     2521 gtaaacagta acatcataga cgccgcggca ggcggatgtc aaggcaaggt tcctggagga
     2581 tcgctggagg tttgctggaa ggagcgtcgg tgtcgccttc gcattccgtc ggatacgcga
     2641 gagccctgct ggcaggtgga gccgccgggg tcgtggcctc cgtggtgtgc ctgggggtgc
     2701 gggtcgtctt gcccaagccc ggaccgcccc cggaggccgt ggccggctcg gcgtttgttg
     2761 cgggagtcct cgggggcctg gtattcggat ggtgggaccg cgtggtgcgc cgcccgggcc
     2821 tcgcactctg gatcaccagc gttgccctgg caacgctgac gagtgcgttc gtcgccatgc
     2881 tgccggctcc ggcgacacgc atcagttcac tcttctaccc ggtagcagga ctggtatttt
     2941 acctggagca ggccgcggcg ctggtgggga ttggacggtt tgcatcgttt cacgtatccg
     3001 ggtcctacat cctcgcagac acggtcctgc actacgtgac cgcgatcgcc gtgagccttc
     3061 tcgttcccct gtgggcgcgt cccgcgccgc gggccccttg atatccacgc gcgtacgcgc
     3121 atgagctgag ggggacgccg cacggcgtcc ccccgctccc cagtatgcta acctccaacc
     3181 tctctctcac cctatattcc taaagtccgt tttgcaccgt ctccgcaagg gcgaagatcg
     3241 aatacgggct cacttcagcg acgtcaaaca aatactaaga gttataagaa tgccaggact
     3301 ccgcaaccta tcactactaa aacacttacc cagaatgcga ttttgtcaaa cttgctgagt
     3361 acatgtcttc ccatgagctg cttcttttga gctaggatcc caagtactat aacaggacct
     3421 attaaaacga agaccaatgc aaccataagg ccgagtataa gtgcgattaa gtgtccgaat
     3481 aacaatggta ttattactgc tggaagggtc tctacaacat agatcttgaa ccacacgtca
     3541 ccccttattc cgagagactc cactatgccc catgcgcttg ccattgatat gactatgag
//
LOCUS       DASQGH010000107         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48551, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000107 DASQGH010000000
VERSION     DASQGH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48551"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VEW91_02080"
     CDS             complement(<1..496)
                     /locus_tag="VEW91_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYM90405.1"
                     /translation="MRAVVLPRASAPLVIEEIPVPAPRAGEVLVRVAACGVCHTDLHV
                     IKGEVAFPPPAVLGHEISGTVVALGPGTRGVREGSRVVSSFILPCGTCVFCAQGRDDL
                     CETFFALNRLKGYLYDGTSRLRRTDGTPLAMYSMGGLAEFAVVPETDVFPLPAEIPQE
                     AACIL"
     gene            691..1830
                     /locus_tag="VEW91_02085"
     CDS             691..1830
                     /locus_tag="VEW91_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90406.1"
                     /translation="MWKKLGGLLFVALLAVPAAGAVQTQAIRVGLAVGITGDISVYGD
                     PIRNGFILAFRQINERSQALIDPILENTGDGRDGAINVFKKFIQRDQVAAILGPVLNS
                     QAFAADPLAVEAGVPVVGVSNTAKGIPEIGLTVFRVSAPEAAQIPKAIATAKAVLNLQ
                     RVVVLYDNADDLTVSGYRTFAEELQKNSVQIADTITFGRGDSDFSTQLTKAQAANPQA
                     IVVSALAREGALLLVQGRNLGITVPFIGGNGFNDPNIITRAGAAAEGLIVGTAWVPSA
                     PSAVNLKFYNAYKDTYKREPNLFAAQAYAGAQVVGEAIRRSKLTGGEDIGTQRTRITE
                     ALKTIKNFETPLSRISITDTRDVNQPRTYVAVVRGGRFVELTPPR"
     gene            complement(1836..3128)
                     /locus_tag="VEW91_02090"
     CDS             complement(1836..3128)
                     /locus_tag="VEW91_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90407.1"
                     /translation="MAVRLVLLVLALAALIPLAPTGPAGAATELKFYYPVSVSGPLAK
                     VMDGMVADFNRLEPGIRVEPVFAGGYFETMAKTQTAVQGGAPPDVAVLLSTDLFTLLD
                     MNAIIPLDTFVEQSGGDRFRQDFFEAFWLNSRISKTIYSIPFQRSTIVLYYNKDAFAK
                     AGLDPNKPPTTWTELREDAQKLTVRDASGTVTRWGVGIPSTGFTYWLFQGFVAEAGGR
                     LASPEGTEVYYHTPQTRRALGFWFALQDARVEPPNVTDWGTLPTEFVAGKFAMVYHST
                     GSLTFVHTNASFPFGTAFMPADKQYGTPTGGGNLYIFKNIPAERQRAAWQFVQWMSAP
                     QQAARWSEASGYVAVRKSAFNIKLYQEYATKFPQALTARDQLPYAQAELSTHNSGQIQ
                     KVLSDNLQSALTRAKTPDQALAAAQEQADQVLSQFRKK"
     gene            complement(3215..4375)
                     /locus_tag="VEW91_02095"
     CDS             complement(3215..4375)
                     /locus_tag="VEW91_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYM90408.1"
                     /translation="MGSPRDVVIVGGGISGTAAAYELARAGASVTLVEKGELASMASG
                     WTLAGVRQSGRHPLELPLAQASVRRWTTLGEELEADLEYRQGGNLRLARTPEEVPVIA
                     RLVEEQRRLGLEIEFLPDPAAVRREAPALSGAVLAASYCPTDGHANPNATVRAFAAAA
                     RRHGAVIRTETEVTGITVHGERVTGVRTAAGSVAADVVVLAAGVHTDRLCAMVGLEFP
                     LRTAHVAVMQTVPLPPLIRQVIGVANADFAGRQEVGGRVRMTGAALPWRWPPGPLGGD
                     DVQPPASEVRVVLDRGVAVLPALAEAKIARLWGGLIDLTPDGLPVIERAPEVEGLVIA
                     AGFSGHGFCLGPITGQLVSELITEGSPSLPLGAFRRARFAGLGRAAAAELHG"
     gene            4502..>5269
                     /locus_tag="VEW91_02100"
     CDS             4502..>5269
                     /locus_tag="VEW91_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90409.1"
                     /translation="MTVRTGQPVRRRAIPGVPRMTRRRFLETALTASVGGAALTIPLS
                     SPSRAAAADNVLRWGQSEADLGTADPHYAAATQDRALADMIFNGLLRFKPGDGSAFEP
                     DLALDFPKPVVIAGKQVWTFKLRKGVMWQPSDKVPAYELTSEDVVYSLQKSADKARSA
                     YSALYTGMTFHALDPYTVSITVDAAVSPNLFYPLVTNYAGGFIVSKRAVEAYGIDGVK
                     TRPVGTGPFMLRRYSPKEKVELAPHPTYFRGRPKLDGI"
BASE COUNT          871 a         1864 c         1746 g          788 t
ORIGIN      
        1 ccaggatgca cgcggcctcc tgggggatct ccgccgggag cgggaacacg tctgtttccg
       61 gcacgaccgc gaactccgcc agtccaccca tgctgtacat cgccagcggc gtgccgtcgg
      121 tgcggcgcag gcgactggtg ccgtcgtaga ggtatccctt caaccgattc aacgcgaaga
      181 acgtctcgca gagatcgtcg cgcccttggg cgcaaaagac gcaggtcccg cagggcagga
      241 tgaagctgct gacgaccctg gagccctccc ggacgccgcg cgttcccggg ccgagcgcga
      301 cgacggtgcc cgagatctcg tgccccagga cggcgggggg gggaaacgcg acctccccct
      361 tgatcacgtg gagatccgtg tggcagacgc cgcatgccgc cacccggacc aggacctcgc
      421 ccgccctggg ggcggggacg ggaatctctt cgatcacgag cggggcgctc gcccggggaa
      481 gcaccaccgc ccgcatctgt tccatcgctt cctcccccga tcgtgggtgc gcgagccccg
      541 ccttcacccg gcgccggcgg gtgcggaggc cgcgtagcgg tcccatcttc aaggcgctac
      601 gccgcctctc ctccggcggc gcgccagctc cagcaaagga aaaccccggt ccccgtcaaa
      661 tcctcacccc gaaaacacag gcggtgcgcg atgtggaaga aactgggggg cctcctgttc
      721 gtcgcgttgc tcgcggtgcc cgccgcgggg gctgtgcaga cgcaggcgat cagggtcggt
      781 ttggccgtcg ggatcaccgg cgatatctcc gtgtatggcg atcctatccg gaacgggttc
      841 attctcgcat ttcggcagat caatgaacgg agccaggccc tgatcgatcc catcctcgag
      901 aacaccgggg atggccgcga cggggcgatc aacgtcttca aaaagttcat ccagcgcgac
      961 caggtggcgg cgatcctggg cccggtgctc aactcgcagg ccttcgccgc ggatccgctc
     1021 gcggttgagg ccggggtccc cgtggtcggc gtgagcaata cggccaaggg aattccggag
     1081 atcggactca cggtctttcg cgtctccgca cccgaagccg cgcagattcc caaggcgatc
     1141 gcgacggcca aggcggtgct caacctccag cgggtcgtcg tcctctacga caacgcggac
     1201 gatttgaccg tctccggcta ccggacgttt gccgaagaac tgcagaagaa cagcgttcag
     1261 atcgccgaca ccatcacctt cggccggggg gacagcgact tttcgacgca gctgacgaaa
     1321 gcgcaggccg cgaaccccca ggcgatcgtc gtgagcgcgc tggcgcggga gggtgccctg
     1381 ctcctcgtcc aggggcgcaa cctcggcatc accgtgccct tcatcggcgg caacggcttc
     1441 aacgacccca acatcatcac ccgcgcgggc gcggcggccg aaggcctgat cgtcgggacc
     1501 gcgtgggtgc cgagcgcccc gtcggcggtg aacctcaagt tctacaatgc gtacaaggac
     1561 acgtacaagc gagaacccaa cctatttgcc gcgcaggcgt acgcgggcgc ccaggtcgtc
     1621 ggcgaggcga tccggaggag caagctgacc ggcggcgagg acatcgggac gcagcgcacc
     1681 aggatcaccg aggcgctcaa gacgatcaag aacttcgaaa cgccgctcag caggatcagt
     1741 atcaccgata cccgagacgt gaaccagccg cgcacgtacg tcgccgtcgt ccgcggcggc
     1801 aggtttgttg aactgactcc tccccggtaa cccggctact tcttcctgaa ctggctcagg
     1861 acctgatcgg cctgctcctg ggcggccgcg agcgcctggt ccggcgtctt ggcgcgcgtg
     1921 agcgccgatt ggaggttgtc ggagagcacc ttctggatct ggccgctgtt gtgggtcgag
     1981 agctccgcct gcgcgtaggg caactgatcc cgtgccgtca gggcctgcgg aaacttcgtc
     2041 gcgtactcct ggtacagctt gatgttgaac gcggacttgc gcaccgcgac gtagccgctc
     2101 gcctcgctcc accgcgccgc ctgttgcggg gcgctcatcc attggacgaa ctgccaggcc
     2161 gcgcgctggc gctctgccgg gatgttcttg aagatgtaca ggttgcctcc acccgtcggc
     2221 gtgccgtact gcttgtccgc gggcatgaag gccgtgccga acgggaacga ggcgttcgta
     2281 tgcacgaacg tcaggctgcc cgtcgagtgg tagaccatcg caaacttgcc cgcgacaaac
     2341 tcggtcggga gggtgcccca gtcggtgacg ttcgggggct cgaccctggc gtcctggagc
     2401 gcgaaccaga acccgagcgc ccgccgcgtc tggggagtgt ggtagtagac ttccgtgccc
     2461 tcggggctcg ccagcctgcc cccggcctcc gcgacgaacc cctgaaatag ccagtaggtg
     2521 aacccggtgc tcggaatgcc caccccccac cgggtcaccg tgcccgaggc atcgcggacc
     2581 gtcagcttct gcgcgtcctc ccggagttcc gtccaggtcg tcgggggctt gttgggatcg
     2641 agtcccgcct tcgcgaacgc atctttattg tagtaaagca cgatcgtgct gcgctggaac
     2701 gggatcgagt agatcgtctt gctgatccgc gagttgagcc agaacgcctc gaagaagtcc
     2761 tgccggaacc ggtctcctcc cgactgttcg acgaaggtgt cgagggggat gatcgcgttc
     2821 atgtcgagca gggtgaacag gtcggtcgag agcagcaccg ccacatccgg gggtgcgccc
     2881 ccctggaccg cggtctgcgt cttggccatg gtctcgaagt acccgccggc aaacaccggc
     2941 tcgacacgaa tcccgggctc gaggcggttg aagtcggcca ccatgccgtc catcaccttg
     3001 gccagggggc cggagacgct caccgggtag tagaacttga gctccgtcgc cgcccccgcc
     3061 ggaccggtcg gggcgagggg gatcagcgcg gcgagcgcga gcactaggag caccaggcgt
     3121 acggccatgg caagccctcc ctccaatgcc tctcatctgc agaataccgg ggaccgcgga
     3181 cccaatcctg cccaggatgg gggggccgcg ccggttaccc gtgcagttcc gccgcggccg
     3241 cgcgccccag gccggcgaac cgcgcccgcc tgaacgcccc gaggggcagg gacggactcc
     3301 cttccgtgat caactcgctc acgagttgcc cggtgatcgg ccccaggcag aacccgtggc
     3361 cggagaagcc cgcggcgatc accagcccct cgacctcggg ggcgcgctca atcaccggca
     3421 gcccatcggg cgtgaggtcg atcagaccgc cccagaggcg ggcgatcttc gcctccgcga
     3481 gcgccgggag caccgcaacc ccccggtcga gcaccacccg gacctcgctc gccggaggct
     3541 ggacgtcgtc cccgcccaat ggacccgggg gccaccgcca gggaagcgca gccccggtca
     3601 tccgcacccg tcccccgact tcctgccggc cggcgaagtc ggcgttggcg accccgatga
     3661 cctgccggat caaggggggg agcggtaccg tctgcatcac cgcgacatgc gccgtgcgca
     3721 gcggaaactc gaggcccacc atcgcgcaga ggcggtcggt gtgcacccct gcggcgagca
     3781 cgacgacatc ggccgcaacc gagccggcgg ccgtgcgaac gcccgtcaca cgctcgccgt
     3841 gcacggtgat gccggtcact tcggtctcgg tgcgaatcac cgcgccgtgg cggcgggccg
     3901 ccgccgcgaa cgcccgcacc gtcgcgttcg ggttcgcgtg gccgtcggtg gggcagtatg
     3961 aggcggcaag caccgcaccg gacagcgccg gcgcctcccg ccggacggcg gcgggatcgg
     4021 gaaggaactc gatctcgagg ccgagacgcc gctgctcctc gacgagccgc gcgatgaccg
     4081 ggacctcctc cggggtccgg gcgaggcgga ggtttccccc ctgccggtac tcaaggtccg
     4141 cctccagttc ctcgccgagg gtggtccagc ggcgcaccga cgcctgcgcg agcgggagct
     4201 cgagcgggtg acggcccgac tggcgcaccc cggcgagcgt ccagccggag gccatgctcg
     4261 ccagctcccc cttctcgacc agcgtgacgg atgccccggc ccgggcaagc tcgtacgcgg
     4321 cggccgttcc gctgatcccg ccgccgacga tgacgacatc gcgtgggctc cccatcggtg
     4381 gctgtgcaat tccgctgcac ccggaggctt tcctccgcga aggtggtgag caggcgatca
     4441 cccgcctgct gtcaaattgg cacgacgacc tcgtatcctg gagcgaacgc tcggaggagg
     4501 cgtgaccgtg aggaccggac agcccgtgcg caggagagcg atcccggggg tgccccggat
     4561 gacccggcgc cggttcctcg aaaccgcgct gaccgcaagc gtgggcgggg ccgccctgac
     4621 catccccctc tccagtccat cccgggctgc cgcagccgac aacgtgctcc ggtggggcca
     4681 gtcggaggca gatctgggga ccgcagaccc gcactacgcg gccgcaaccc aagaccgcgc
     4741 gctcgcggac atgatcttca acggcctgct gcgcttcaag cccggcgacg gctcggcgtt
     4801 cgagccggac ctggccctcg acttccccaa gccggtcgtg atcgcgggca aacaggtctg
     4861 gacgttcaag cttcggaagg gggtcatgtg gcagccgagc gacaaggtgc ccgcatacga
     4921 attgaccagc gaggatgtcg tctactcgct gcagaagtcc gcggataaag cgcggtcggc
     4981 ctactcagcc ctctacaccg gaatgacctt ccacgccctc gatccctaca cggtcagcat
     5041 caccgtcgat gcggccgtct ctcccaacct gttctatccc ctggtcacca actacgcggg
     5101 cggcttcatc gtgtcgaagc gggcggtcga ggcctacggc atcgacggcg tgaagacgcg
     5161 cccggtcggc accggcccgt tcatgctccg ccggtacagc ccgaaagaga aggtcgaact
     5221 cgcgccacac cccacctatt tccggggccg gcccaagctc gacggcatc
//
LOCUS       DASQGH010000108         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45766, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000108 DASQGH010000000
VERSION     DASQGH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45766"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VEW91_02105"
     CDS             <1..1029
                     /locus_tag="VEW91_02105"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HYM90410.1"
                     /translation="QAEEVLVRMAAGGVCHTDLHVMTGHLFAPLPAVLGHEGAGTVAE
                     VGPGVESVRPGDHVIPLWRLSCGECEYCTGGRPALCPAGTEIRMTGRLPDGTTRFALG
                     DQEIKHFAGVSSFSEFSVIPEKALIRIPDDLPLDRAALFGCAVICGVGAVLNAARVRP
                     GSSVAVFGAGGVGLNVIQGAALAGAGKIIAVDLLEGKLEYARRFGATHTVNASSGNPV
                     EQVRALTGGRGVDYAFEAIGVPAVMRQAYDTLAKRGMAVVIGVTPMTAEVSVPVMSLV
                     FEERTLSGSVYGASRPRIDIPMLIDLYRAGKLKVDELLTRTYPFDQINEAYAALERGE
                     VARTVVTF"
     gene            complement(1121..>1681)
                     /locus_tag="VEW91_02110"
     CDS             complement(1121..>1681)
                     /locus_tag="VEW91_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM90411.1"
                     /translation="DGREEDYTALFARCTEVMEAIRTLPKPVIAQVHAIATAAGCQLA
                     ASCDLVVASEDATFATPGVRIGLFCTTPGVALARAVGPKKAMEMLLTGRPINAREAEQ
                     AGLVNRVVPADRLAEETMALARQIITASGYTLGIGKRAFYEQVPLDRPHAYEVASRVM
                     VENALAPDAQEGMKAFLEKRPPRWRS"
BASE COUNT          241 a          550 c          609 g          281 t
ORIGIN      
        1 caggccgagg aggtgctcgt ccggatggcg gccgggggag tgtgccatac cgacctgcac
       61 gttatgaccg ggcacttgtt cgcgcccctg ccggcggtgc tcgggcacga gggggcggga
      121 actgtggcgg aggtggggcc cggcgtggaa tcggtccggc ccggggacca cgtcatccca
      181 ttgtggcggc tgagctgtgg ggagtgcgag tactgtaccg ggggacggcc ggcgctctgc
      241 ccggccggca cggagatccg gatgaccggg cgcctcccgg acggcaccac acgcttcgcg
      301 ctgggagatc aggagatcaa gcacttcgcc ggcgtgtcga gcttctcgga gttctccgtg
      361 atcccggaga aagcgctgat caggattccg gacgacctcc ccctcgaccg ggccgccctc
      421 ttcggctgcg cggtgatctg cggggtgggc gcggtgctca acgccgcacg ggtcaggccg
      481 gggagcagcg tcgcagtctt tggcgccggc ggggtcgggc tcaacgtcat ccagggggcc
      541 gccctggcgg gcgccgggaa gattatcgcc gtggacctgc tggagggcaa actggagtat
      601 gctcggaggt tcggtgccac gcatacggtg aacgcatcgt ccgggaatcc cgtggagcag
      661 gtccgagcgc tcaccggggg gcggggcgtg gactatgcat tcgaagcgat cggtgtcccc
      721 gcggtgatgc gacaggcgta cgacacgctg gctaagcggg ggatggcggt cgtcatcggc
      781 gtcaccccga tgacggcgga ggtgtccgta cccgtgatgt cgctcgtttt cgaggaacgc
      841 actctgtcag gatcggtgta cggggcgagc cgcccccgca tcgacattcc catgctcatc
      901 gatctttatc gtgcgggcaa actcaaggtc gacgagctgc tgacgcggac gtatccgttc
      961 gatcagatca acgaggctta cgccgcgctc gaacgcggcg aggtggcacg gaccgttgtg
     1021 accttctgac cgtcctccgg gggcccgggg aagtcccact cgcccgattg ccggatggct
     1081 tcggtggccc gctcagcgga gatccaaatt tatcgcacgc tcaggaccgc catctcggcg
     1141 gccgcttctc cagaaacgct ttcatccctt cctgggcatc gggcgccagg gcgttctcga
     1201 ccatgacccg cgacgccacc tcgtacgcgt gcgggcggtc gagggggact tgttcgtaaa
     1261 acgcccgctt gccgatgccg agcgtgtacc cgctggcggt gatgatctgc cgggcgagcg
     1321 ccatcgtctc ctcggcgagc cggtccgccg gcaccacgcg gttcaccagc cccgcctgtt
     1381 cggcttccct ggcgttgatc ggcctgccgg tgagcagcat ctccatcgcc ttcttcggcc
     1441 cgacggcgcg ggcgagcgcg acgccggggg tggtgcagaa gagcccgatc cgcacgccgg
     1501 gggtcgcgaa cgtggcgtcc tcggaggcga cgacgaggtc gcagctcgcc gccaactggc
     1561 agccggccgc cgtggcgatc gcgtgcacct gggcgatcac cggcttgggg agcgtccgga
     1621 tcgcctccat cacctcggtg cagcgcgcaa agagcgccgt gtagtcctcc tcgcggccgt
     1681 c
//
LOCUS       DASQGH010000109         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11775, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000109 DASQGH010000000
VERSION     DASQGH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11775"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VEW91_02115"
     CDS             complement(<1..337)
                     /locus_tag="VEW91_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HYM90412.1"
                     /translation="MAEHHKVVSSDEWIEARKQLLAKEKEFTRLRDRLSQQRRDLPWE
                     HVDKPYVFEGPNGKETLPKLFDGRSQLIVYHFMFAPDWEAGCPHCSRWADNFNGIIVH
                     LNQRDVTMVA"
     gene            complement(321..818)
                     /locus_tag="VEW91_02120"
     CDS             complement(321..818)
                     /locus_tag="VEW91_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYM90413.1"
                     /translation="MTEQERLDRLVIRRMMPATREAVFAAWTDPESMREWMCPGDAVT
                     AEAELDLRVGGSFRIIMKSPTRAYDHTGEYLVVEPPSKLAFTWISKGTDNQPTIVTVE
                     LFERGNECELVLTHERFPTADAVRRHRSGWGQIADRLAEHLRQRAGVRRAQTNLKGGP
                     HGGAS"
     gene            complement(838..1179)
                     /locus_tag="VEW91_02125"
     CDS             complement(838..1179)
                     /locus_tag="VEW91_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020810537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYM90414.1"
                     /translation="MVEYRSARLDRVFAALADPTRRSILDALSRRPATITELARPFPV
                     SLNAVSKHVKVLERAGLVRREVVGREHHCRVDARPFRRAAAWLEHYRGFWEARLDALE
                     QHLTARRTRPT"
     gene            complement(1213..1749)
                     /locus_tag="VEW91_02130"
     CDS             complement(1213..1749)
                     /locus_tag="VEW91_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90415.1"
                     /translation="MRRWDEWLEAWFARRFAVRPIGGGGYIFKLGLVRYRGPALTFGD
                     GTVVRRGDLVGELHIDNRRAAALHEGGKGGFRYRREVFRVLPVLAQDLRTRPEYQAVQ
                     AIGGPSLFWEEATHAGFEHWPLPAFTRWWLTWWERFLLARFHPQGRARLGEGHRTELR
                     QAWMTRRTLLERFGKDAS"
     gene            complement(1790..2098)
                     /locus_tag="VEW91_02135"
     CDS             complement(1790..2098)
                     /locus_tag="VEW91_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HYM90416.1"
                     /translation="MAILAISVTPIGTGTPSVGAYVADALRVIEQSGLRYRLNAMHTE
                     VEGELEEVLRLIPAIHHACFARGAKRVSTVIKIDDRRDAPSSIEGKVRSVEARLRPAG
                     "
     gene            2292..3758
                     /gene="pyk"
                     /locus_tag="VEW91_02140"
     CDS             2292..3758
                     /gene="pyk"
                     /locus_tag="VEW91_02140"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYM90417.1"
                     /translation="MPAPEAPRPQPLLPRRTKIVVTIGPASASERVLRQLLDAGMDVA
                     RLNFSHGTRAAHGRTIRLLRKLAREAGAPLGILQDLQGPKIRVGRLAAPIRLLEGAEV
                     VLTTRRVQGTDGRIPIPFPGLARMVRRGGRILLRDGSIELLALECRGREIRCRVVRGG
                     LLSEHQGVNLPGARFRVPSLTRKDAADLRFGLRCGVDYVALSFVRSANDLHHARRAVR
                     RLGGRVPLVAKLEKAEAIAHLEDIIAAADGVMVARGDLGVELPPEQVPLIQKRIIRRA
                     NEQAVPVITATQMLESMVRQERPTRAETSDVANAILDGTDAVMLSEETAVGRYPVETV
                     KMMARIARAVEAGPRLWAEPRCAGPSRRGFAHAIAAATRTLAADLRLDLIVALTATGR
                     TAQILSQLRPPVLVLACTEDEGLARRLALYWGVRPFVTRFRPHTEAMIRFLDRELVRR
                     GLARPGAAVAIVGSVPIIARGRTNFVQLHRVGQAGPAG"
     gene            3841..>4527
                     /locus_tag="VEW91_02145"
     CDS             3841..>4527
                     /locus_tag="VEW91_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90418.1"
                     /translation="MPEARKVIILGSGSAGLTAAIYAARAQLRPLVIAGNQRGGQLTL
                     TTDVENYPGFAEGVQGPELMEIMRRQAERFDAEFLDEDATAVNFRRRPFDVTLAHETH
                     RAEAVIIATGAGTNWLGVPNEQRLIGHGVSSCAPCDAFFFREKEVAVVGGGDSAMEEA
                     LVLTKFATKVTIVHRRDQFRASKIMADRALHHEKIQVLWNTVVDDILGNGSVTGLRLH
                     DVTTGAQREFR"
BASE COUNT          665 a         1611 c         1584 g          667 t
ORIGIN      
        1 ccgccaccat ggtcacatcg cgctggttca ggtgcacaat gatcccgttg aaattgtcgg
       61 cccaacggga gcagtgcggg catcctgcct cccaatcggg cgcgaacatg aagtggtaga
      121 cgatcagctg gcttcggcca tcaaaaagct tgggcaacgt ctccttgccg ttcggccctt
      181 caaagacgta cggcttgtcg acatgctccc agggcagatc gcgtcgctgc tggctgagcc
      241 ggtcgcgcag gcgtgtaaat tccttctctt tggccaggag ctgtttgcgc gcttcaatcc
      301 actcgtcgct cgacacgacc ttatgatgct ccgccatgag gccccccctt gagatttgtt
      361 tgcgccctcc gcaccccggc gcgctgccgg aggtgctccg ccagacggtc ggcgatctgg
      421 ccccacccgc tccggtggcg ccgcacggcg tcggcggtgg ggaagcgctc gtgggtgagg
      481 acgagctcgc actcgttccc gcgctcaaag agctccaccg tgacgatcgt gggctggtta
      541 tcggtgccct tcgagatcca cgtgaaggcg agcttcgatg ggggctcgac gaccaagtat
      601 tctcccgtgt gatcgtacgc ccgggtcggg ctcttcatga tgatccgaaa tgagcctccc
      661 acccgcaggt cgagctccgc ctcggccgtc acggcatcgc cgggacacat ccactccctc
      721 atgctctccg ggtccgtcca ggcggcgaac acggcctcgc gcgtcgcggg catcatccgc
      781 cgaatgacga gccggtccaa tcgctcctgc tcggtcaccg tgccctccgg ggtgcgttta
      841 tgtgggccgc gtgcgccggg ccgtgagatg ctgctccagc gcgtccagcc gggcctccca
      901 gaacccgcgg tagtgctcga gccacgccgc ggcccggcgg aacggcctcg cgtccacgcg
      961 gcagtggtgc tcgcggccga ccacctcgcg gcggacgagc ccggcccgct cgaggacctt
     1021 cacatgcttc gagacggcgt tgagggagac cggaaacggc ctggcgagct cggtgatggt
     1081 ggccggcctg cggctgaggg catccaggat cgagcggcgc gtgggatcgg ccagggccgc
     1141 gaagacccgg tccagccggg ccgatcgata ttcaaccata cggttgaata ataaacgccg
     1201 gacgcgcggt tgtcaagagg cgtccttccc gaaccgctcc aggagcgtgc gccgcgtcat
     1261 ccaggcctgc cgcagctccg tccggtgccc ctcgccgagg cgcgcgcggc cctggggatg
     1321 gaagcgagcg aggaggaacc gctcccacca cgtgagccac catcgagtga acgccgggag
     1381 cggccagtgc tcgaacccgg catgggtggc ctcctcccag aagaggctcg gcccgccgat
     1441 cgcctggacc gcctgatact cgggccgcgt gcgcaggtcc tgggccagca cggggagcac
     1501 gcggaagacc tcgcgccggt agcggaaccc tcccttgccc ccttcgtgca gcgcggcggc
     1561 gcgccggttg tcgatgtgca actcccccac gagatcgccc cgccggacga cggtgccgtc
     1621 cccgaaggtc agcgccggcc cgcggtaccg gacgagaccc agcttgaaga tgtacccgcc
     1681 cccgccgatc gggcggacgg caaagcgccg cgcaaaccag gcctccagcc actcgtccca
     1741 gcgcctcacc ggaccggctc cctggccgcg gacgccgtgc gcccccggct caccccgcgg
     1801 ggcgtagccg cgcctccacc gaccgcacct tcccttctat agaagaaggc gcgtcccggc
     1861 ggtcgtcgat cttgatgacg gtggagaccc gcttcgcccc gcgggcgaag caggcgtggt
     1921 ggatcgcggg gatgaggcgc aggacctcct ccagctcccc ctcgacttcc gtgtgcatcg
     1981 cgttgagccg gtaacgcagg ccgctttgtt cgatcacccg cagggcgtct gcgacgtacg
     2041 cccccacact gggggttccc gtcccaatcg gagtcacgct gatcgccagg atcgccacgg
     2101 ccgccctccc cgtgctgctg gtgtcggagc catgccccac cgggcgcgaa gcccgggggg
     2161 ctccgttcgt tcccgagtct agcacacgaa ggctcctgac ggtcccgcct ctagagtatg
     2221 atgtggggcg gaggaggtgg cgatgccgca cgcgtggtgg ccaaggtgga ggccgcggga
     2281 ggactcaacc ggtgcctgcg cctgaggccc ctcgtcccca accgctcctg ccccggcgga
     2341 cgaagatcgt ggtgacgatc gggccggcca gcgcgagcga gcgggtgctc cggcagctcc
     2401 tcgatgccgg catggacgtc gcgcggctca acttctctca cggcacccgc gccgcccacg
     2461 gccgcacgat ccggctgctc cggaagcttg cccgggaggc cggggcgccc ctggggatcc
     2521 tccaggacct gcagggcccc aagatccggg tcggacggct cgcggcgccg atcaggcttc
     2581 tagagggcgc cgaggtcgtg ctcacgaccc ggcgcgtcca gggcacggat gggcggatcc
     2641 cgatcccgtt ccccggcctc gcgcgcatgg tgcggcgggg cggtcgcatc ctgctgcggg
     2701 acggcagcat tgaactcctg gcgctcgaat gccggggacg cgagatccgg tgccgggtcg
     2761 tgcgcggggg gctgctgagc gagcatcagg gggtcaacct cccgggcgcc cggttccggg
     2821 tcccgtcgct cacccggaaa gacgccgccg acctcagatt cggcctccga tgcggggtgg
     2881 attatgtcgc gctcagcttc gtgcggagcg ccaacgacct ccaccacgcc cggcgggccg
     2941 tgcggcgtct cggagggcgc gtgcccctcg tggccaagct ggagaaagcc gaagcgatcg
     3001 cccacctgga ggacatcatt gcggcggccg acggcgtgat ggtcgcccgc ggcgatctcg
     3061 gcgtcgagct gcctccggag caggtgcccc tgatccaaaa gcggatcatc cgccgcgcga
     3121 acgagcaggc cgtgccggtc atcaccgcca cccagatgct ggagtcgatg gtgcgccagg
     3181 agcggcccac ccgggcggag acctcggatg tcgccaacgc gatcctggac ggcaccgacg
     3241 ccgtgatgct gtcggaggag accgcggtcg gccggtatcc ggtcgagacg gtgaagatga
     3301 tggcccggat cgcccgggcg gtggaagccg gcccccggct atgggcggag ccccggtgcg
     3361 cgggcccgag ccgccggggg ttcgcccacg cgatcgcggc cgccaccagg acgctggcgg
     3421 cggacctgcg gctcgacctc atcgtggcct tgacggcgac cgggcgcacg gcgcagatcc
     3481 tgtcgcagct acgcccgccg gtcctggtgc tggcctgcac ggaggacgag gggctggcgc
     3541 ggcgcctggc cctgtactgg ggggtgcgcc cgttcgtgac gcggttccgg ccgcacacgg
     3601 aggcgatgat ccggtttctc gaccgggagc tcgtccggcg cggcctcgcc cgcccggggg
     3661 ccgccgtcgc gatcgtcggg tccgtgccga tcatcgcccg cggacgaacg aacttcgtcc
     3721 agctccaccg ggtgggacag gccgggccgg ccggatagcc ccgggcggcc cgggaagaat
     3781 cgcggccgcc acgtcgttcc tgtggtaggc agtgaggagg agatcctggg ggtgcacgcg
     3841 atgccggagg cacgcaaggt catcatcctc ggctccggat cggccgggtt gaccgcggcg
     3901 atctacgccg cccgggccca actgcggccg ctggtcatcg ccgggaacca gcgggggggg
     3961 cagctcaccc tcaccaccga cgtggagaac tacccgggct tcgcggaggg cgtccagggt
     4021 cccgagctca tggagatcat gcgccggcag gcggagcggt tcgacgccga gttcctcgac
     4081 gaggacgcca ccgccgtcaa cttccgccgc cggccgtttg acgtgacgtt ggcccacgag
     4141 acccatcggg ccgaggcggt gatcatcgcc accggggccg ggaccaactg gctcggggtg
     4201 ccgaacgagc agcgcctgat cggccacggg gtgtcctcct gcgccccgtg cgacgccttc
     4261 ttcttccgcg agaaagaggt cgccgtcgtg gggggtgggg actccgccat ggaagaggcg
     4321 ctcgtcctca ccaagttcgc caccaaggtg acgatcgtcc accggcggga ccagttccgg
     4381 gcgagcaaga tcatggcgga tcgcgccctg caccacgaga agatccaggt cctctggaac
     4441 accgtggtcg acgatatcct cggcaacggc tcggtcaccg gcctgcggct gcacgacgtc
     4501 acgaccggcg cgcagcgcga gttccgg
//
LOCUS       DASQGH010000110         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_406, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000110 DASQGH010000000
VERSION     DASQGH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEW91_02150"
     CDS             <1..510
                     /locus_tag="VEW91_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYM90419.1"
                     /translation="VAGAVRFVKTRQGGTAHRIGVFGLSMGAVAAILAAAACPEIGAV
                     VSDSSYTRLDHAVRWAYRGLWGPAALLFYAPTRWLGERLMGTAMPDVSPLDAIGRIRP
                     RPILLIHGTQDRLIDPADAQALHRRAGDPKTLWLVEGADHAETRAAAAGEYDTRVAGF
                     FRQHLGGRL"
     gene            507..1775
                     /locus_tag="VEW91_02155"
     CDS             507..1775
                     /locus_tag="VEW91_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HYM90420.1"
                     /translation="MTGTRAAGATPVTEFKPAKPNRLFIHAMRVLNRLYVVPRARLVC
                     DARELERLKQLPPGVIITPNHSDYSDAIVVTELGRRAGRLFMFMATRESFDTNCGING
                     RVLQSMGGFSVNRGGENAGCQQFAKGVVKAGRYDLLIFPEGEVYLLNDLVMPFKPGVA
                     MLALEVASENLKEGRPDRPVTIVPLAIKYRYLVDILPALRGIVERLEATIFGAVRGGA
                     LYDRISALGIELLNRKEQEYGVRPAADHDLYARVDAVRRHLLETLERRYLGRVRDEFA
                     FDRARRLIIHLLEELAHLRDRGDLPPHEKSATEADLRKDLRWAQTAARSVSFAEDYLI
                     ARPTPERLAETLAKLEREIYGREAFPPRGRRRVTIKIGTPIDVRLYAAEYAERRTRKE
                     GILRLVRDLQASIQAMIDALNAEPDEGVPP"
     gene            1772..2566
                     /locus_tag="VEW91_02160"
     CDS             1772..2566
                     /locus_tag="VEW91_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYM90421.1"
                     /translation="MKLAQTRAVRWAARLSDRMHAHQVSEQATVIAFNIVYSLFPLAL
                     SLTAIGGFLVRGAAERAVLLAEIRAAFPAQLAREMVDVVNAAGSYPGLFGLVGFASLL
                     LAGSNLFAAIEVSFARIFGVPPRGIVHQRAVAVLMILAFSVLLIVSVTASDLAVFLVG
                     GPYLGLAGGWVVATLMQLMIYAVIPNVKVPFRALWPGAVLAGTAMQVITLVFPLYIRF
                     FAGFNRFGDAFSLVFLVMTWAYFLAFILLAGAEVNAMRYADAGGGR"
     gene            2719..3363
                     /locus_tag="VEW91_02165"
     CDS             2719..3363
                     /locus_tag="VEW91_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYM90422.1"
                     /translation="MRIGILGTGDVGRALGNGCISRGHDVMMGSREATNEKARAWAAE
                     AGPRASAGTFAEAAKFGEVAVVATLWSGTENALRLAGPENLAGKVVIDATNPLRFTEG
                     KPPSLALGHTDSGGEQVQRWLTGARVVKAFNIVGNAHMVDPRFPGGPPDMFICGNDAQ
                     AKQTVTALLTAFGWNTIDIGGIEGARLLEPLCILWVLYGVRSGSWNYAFKLLRR"
     gene            complement(3400..>4360)
                     /locus_tag="VEW91_02170"
     CDS             complement(3400..>4360)
                     /locus_tag="VEW91_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYM90423.1"
                     /translation="LSPSPAVIVIPQRGRSGQHGQVLGMGFERITGWIRVGPLAAVVL
                     WGGIYPGAKLGLQEIPPLSFTYLRVVLATIVLVAVSSRARPAGPPRSVWQPALRAGAA
                     QTAFQLLLIAGLARTTAGTSAILLAASPLIVAGWLAFKAREHVPGQHWLGLLLGLAGV
                     AFVVRGGMTSLDPTHLSGNLIALGAAGAWAWYSLTIGPLVEAGGAMRATGWAMAIAGA
                     LITPVALGQVIHLPWRGISWVAWAGLVYGATAGMVVAMALWGRSVQTLGPRQTMVYAY
                     LEPVSAVIIAAVLLGETLRPIQGVGALLTFAGVWLGSARQPER"
BASE COUNT          638 a         1501 c         1563 g          658 t
ORIGIN      
        1 gtggcgggcg ccgtgcggtt cgtgaagacg cgccaggggg ggacggccca tcgaatcggc
       61 gtctttgggc tgtcgatggg ggcggtcgcg gccattctgg cagcggccgc gtgcccggag
      121 atcggcgcgg tggtgtccga cagcagctac acccgtctgg atcacgcggt gcgctgggcc
      181 taccggggcc tctggggacc ggcggccctc cttttctatg cccccacgcg ctggctgggg
      241 gagcggctga tgggcacggc gatgcccgac gtctcgccgc tcgatgcgat cggccggatc
      301 cgtccgcgcc ccatcttgct catccacggc acgcaggacc ggctgatcga ccccgccgac
      361 gcccaggccc tccatcggcg ggccggggat cccaagaccc tctggctggt ggaaggcgcc
      421 gatcacgccg agacgcgggc cgccgcggcc ggggagtacg acacgcgcgt cgccgggttc
      481 tttcgacagc acctcggggg ccggctgtga cgggcacccg cgcggcgggc gccacgccgg
      541 tgaccgagtt caagccggcg aagcccaacc ggctgttcat ccacgccatg cgggtgctca
      601 accggctcta cgtggtgccc cgcgcccggc tggtgtgcga cgcccgcgag ctcgagcggc
      661 tcaagcagct ccccccgggt gtgatcatca cgcccaacca ctcggactac tccgatgcca
      721 tcgtcgtgac cgaactgggg cggcgcgcgg gccgcctctt catgttcatg gcgacgcgcg
      781 aatcgttcga tacgaattgc gggatcaacg ggcgggtgct gcagtcgatg ggcgggttct
      841 ccgtcaatcg gggcggggag aacgccgggt gccagcagtt cgccaagggg gtcgtcaagg
      901 caggccggta cgacctcctc atcttcccgg agggcgaggt gtacctgctg aacgatctcg
      961 tcatgccgtt caagccgggc gtggcgatgc tcgcgctcga ggtggccagc gagaacctca
     1021 aggaaggccg gccggaccgg ccggtcacga tcgtcccgct cgccatcaag taccgctacc
     1081 tggtggatat cctgcccgcg ctcaggggga tcgtggagcg gctcgaagcg accatcttcg
     1141 gtgcggtgcg gggaggagcg ctgtacgacc ggatctccgc gctcgggatc gagttgctca
     1201 accgcaagga acaggagtac ggcgtccgcc ccgccgccga ccacgacctc tacgcgcgcg
     1261 tggacgccgt gcgccggcac ctgctggaga cgctggagcg ccgctatctg ggcagggtac
     1321 gggacgaatt cgcgttcgac cgcgcccgcc ggctgatcat ccatctcctg gaggagctgg
     1381 cgcacctgcg cgaccgcggc gatctccccc cgcacgagaa gtcggccacg gaggcggatc
     1441 tgcggaagga tctccggtgg gcgcagaccg cggcccgatc ggtgtccttc gccgaggact
     1501 atctcatcgc ccgtcccacc ccggagcgcc tggcggagac gctggccaaa ctggaacggg
     1561 agatctacgg ccgggaggcg ttcccgcccc gggggcggag gcgggtgacg atcaagatcg
     1621 gcaccccgat cgacgtgcgg ctctacgccg ccgagtacgc cgagcgccgg acccgcaagg
     1681 aggggattct ccgcctcgtc cgcgatctcc aggccagcat ccaggcgatg atcgacgccc
     1741 tcaacgctga gccggatgag ggggtgcccc catgaagctg gcccagaccc gggcggtgcg
     1801 gtgggcggcc cgcctgtccg accggatgca cgcgcaccag gtgagcgagc aggccaccgt
     1861 gatcgcgttc aacatcgtgt acagcctgtt ccccctggcg ctgtcgctga ccgcgatcgg
     1921 cggcttcctc gtccgggggg ccgcggagcg ggccgtcctc ctggccgaga tccgggcggc
     1981 gttccccgcg cagctggccc gggagatggt cgacgtggtc aacgccgcgg ggagctaccc
     2041 cgggctcttc gggctcgtgg ggtttgcgtc gctgctcttg gccggatcga atctcttcgc
     2101 cgcgatcgag gtgagctttg cccgcatctt cggggtgccg ccgcggggga ttgtgcacca
     2161 gcgggccgtg gccgtgctga tgatcctggc gttttccgtc ctgctgatcg tgtccgtgac
     2221 cgcgtcggac cttgcggtgt tcctcgtcgg aggcccgtac ctggggctgg ccggcggctg
     2281 ggtggtcgcg acgctgatgc agctgatgat ctacgcggtg atccccaacg tcaaggtccc
     2341 gttccgggcg ctctggccgg gggcggtgct ggccgggacc gccatgcagg tcatcaccct
     2401 cgtgtttccc ctctacatcc gattcttcgc ggggttcaac cgcttcggcg atgccttcag
     2461 cctggtcttc ctggtgatga cgtgggccta tttcctggcg ttcatcctgc tcgccggcgc
     2521 ggaggtcaac gccatgcggt acgccgacgc cggtgggggg cgctagcggg gatatcgtag
     2581 acgccccgat cgatcttcgg tacggccgct gctgctatcg cccatgggta acttggaccc
     2641 tgttctcaca tctgaggacg tggcgcgaga tccccgtcgt tgaccgtgtc ggtcattcga
     2701 ggataggagg aggtcgggat gcgaatcggg atcctgggaa cgggcgacgt cgggcgcgcg
     2761 ctgggcaacg gatgcatctc gcggggccac gacgtcatga tgggatcgcg ggaggccacc
     2821 aatgagaagg cccgggcgtg ggctgcggaa gccggccctc gcgcctctgc cgggacgttt
     2881 gcggaggcgg ccaaatttgg cgaggtcgcc gtcgtcgcca cgctgtggag cgggaccgag
     2941 aacgccctgc ggctggcggg gccggagaac ctggcgggca aggtggtcat cgacgccacc
     3001 aacccgctca ggttcacgga aggaaagccc ccctcgctcg cgctcggcca caccgactcc
     3061 ggcggcgagc aggtccagcg gtggctgaca ggggccaggg tggtcaaggc cttcaacatc
     3121 gtgggcaacg cccacatggt cgacccccgg ttccccggcg ggccgcccga catgttcatc
     3181 tgcggcaacg acgcccaggc gaagcagacg gtcaccgccc tgctcaccgc gttcgggtgg
     3241 aacacgatcg acatcggagg catcgagggg gcccggcttc tcgagccgct gtgtatcctg
     3301 tgggtgctct acggggttcg ctcagggtcg tggaactatg cgttcaagct tctgcgccgg
     3361 taggcgggtg cgcgccgcga gggcggcgct cgagcgcggt caacgctcag gttgccgggc
     3421 cgacccgagc cacaccccgg caaaggtcag cagcgcgccc acgccctgaa tcggacggag
     3481 cgtctcgccg agcaatacgg cagcgatgat gaccgccgag accggctcca ggtaggcgta
     3541 caccatcgtc tgccgcggcc cgagcgtctg caccgacctc ccccacagcg ccatcgccac
     3601 gaccatgccg gcggtcgcgc cgtacaccag gccggcccag gcgacccacg agatcccccg
     3661 ccacgggagg tggatcacct ggccgagcgc aacgggggtg atgagggccc ccgcgatcgc
     3721 catcgcccac ccggtcgccc gcatcgctcc acccgcctcg accagcgggc cgattgtgag
     3781 gctgtaccat gcccacgccc cggccgcgcc gagggcaatg agattcccgc tcaggtgcgt
     3841 cgggtcgagg ctggtcatcc ctccgcgcac aacgaacgcg acccccgcca ggccgagcag
     3901 caggccgagc cagtgctggc cggggacatg ctcgcgggcc ttgaacgcca gccacccggc
     3961 cacgatcagc ggcgaggcgg ccagcaggat ggcgctcgtc cccgccgtcg tccgcgccag
     4021 tccggcgatg aggaggagct ggaacgcggt ctgcgccgcg ccggcgcgga gcgccggctg
     4081 ccacacagac cggggcggcc cggccggccg cgcgcggctg gacaccgcga cgagcacgat
     4141 cgtggcgagg acgacgcgca ggtacgtgaa ggacaggggt gggatctcct ggagccccag
     4201 cttggcgccc gggtagattc cgccccagag aacgacggcg gcaagcggcc ccacgcggat
     4261 ccatccggtg atcctctcga agcccatgcc gaggacttgg ccgtgctgcc cgctgcgccc
     4321 tctttgggga atgacgatga cggccgggga tggggataga
//
LOCUS       DASQGH010000111         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54153, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000111 DASQGH010000000
VERSION     DASQGH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54153"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VEW91_02175"
     CDS             <1..1324
                     /locus_tag="VEW91_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM90424.1"
                     /translation="IGRAWMAIREDEVAAGAMGVNRTRLKLLAFAVGAAFAGATGTFY
                     VAKLTTATPDMFQFPVSVMVLVMVVLGGMGSVTGVVLGALILQLLQSWFLQDLTQWTH
                     ALGRLVHSQGLQQVELVQTIELFFGIILVLMMLYRRQGLIPATRTTAALSVVEQAAAA
                     PSRGGIAVTLSRFEQPKVRAGTPMLETHSLTKRFGGITAVRAVDLAVMPDTIVGLIGP
                     NGSGKTTFFNLVTGLDTPDSGRVLFMGKDVTGLPAHAIVEKGIARTFQTLRLFHNMTV
                     LENVLVGMHARTRASAVGAVLRPRSVRAEEAEAREWAMEVLRIFGNRLVPRVNHAAGS
                     LSYANRRRLEIARAIAAQPRLLLLDEPTAGMNPAETLELADQIRSLRDLGITVLLIEH
                     KLNVVNEISDKVAVLDYGEKITEGSAAEVQRNDDVIRAYLGRTAAARV"
     gene            1278..>1818
                     /locus_tag="VEW91_02180"
     CDS             1278..>1818
                     /locus_tag="VEW91_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90425.1"
                     /translation="MSSGPTLDGPLLLEFRDVDTYYGELHVLKRVNYEIRAGEIVCLL
                     GGNASGKSTTMKTVLGLVRPARGTVLYRGEPINTLPTSERVRRGIAPVPEARRLFPKM
                     TVYENLEMGAYTRDNLVEIQEDVDRVYELFPRVKERRNQLAGTLSGGEQQMVAIGRAL
                     MARPNLVCMDEPSMGLSPAF"
BASE COUNT          294 a          589 c          649 g          286 t
ORIGIN      
        1 gatcggtcgg gcgtggatgg cgatccgcga ggacgaggtc gccgcagggg cgatgggggt
       61 caaccggacg cggctgaagc tcctggcatt tgccgtcggg gccgcgttcg ccggcgccac
      121 ggggactttt tacgtcgcga agctcacgac cgccaccccc gacatgtttc agttcccggt
      181 ctcggtgatg gtgctcgtga tggtggtgct ggggggcatg ggaagcgtca ccggcgtcgt
      241 attgggggcg ctcatcctgc aactgctcca gtcctggttc ctccaggatt tgacccaatg
      301 gacccatgcc ctgggacggc tcgtgcacag ccaggggctg cagcaggtcg agctcgtgca
      361 gacgatcgag ctcttcttcg ggatcatcct cgtgctgatg atgctgtacc ggcgccaggg
      421 gctgatcccg gccacccgga ccaccgcggc gctctccgtg gtcgagcagg cggcggcggc
      481 gccatcccgg gggggcatcg cggtgaccct cagccggttc gagcagccca aggtgcgggc
      541 ggggacgccg atgctggaga cccacagcct gacgaagcgg tttggaggga tcaccgcggt
      601 ccgagccgtg gacctcgccg tcatgccgga cacgattgtc ggcctgatcg ggccgaacgg
      661 gtcgggcaag acgacgttct tcaaccttgt gaccgggctg gacacgccgg atagcgggcg
      721 ggtcctcttc atggggaagg atgtcacggg gctccccgcg cacgccatcg tggagaaggg
      781 gatcgcgcgg acgttccaga cgctgcggtt gttccacaac atgaccgtcc tcgagaatgt
      841 gctcgtcggc atgcacgccc gcacccgggc cagcgcggtg ggtgcggtgc tccggccccg
      901 ctcggtgcgc gccgaggagg ccgaggcgcg ggagtgggcg atggaggtcc ttcgcatctt
      961 cggcaaccgg ctggtcccgc gggtcaacca cgcggcgggc agcctctcct acgccaaccg
     1021 gcggcggctc gagatcgcca gggcgatcgc cgcgcagccc aggctgttgc tcctcgacga
     1081 accgacggcc gggatgaacc ccgccgagac cctggagctc gccgaccaga tccggagcct
     1141 ccgagacctc gggatcaccg tgctcctcat cgagcacaag ctgaacgtgg tcaacgagat
     1201 ctccgacaag gtggccgtgc tcgactacgg ggagaagatc accgaaggca gcgcggcgga
     1261 ggtgcagcgc aacgacgatg tcatccgggc ctaccttgga cggaccgctg ctgctcgagt
     1321 ttagggacgt cgacacctac tacggggagc tccacgtcct caagcgggtc aactacgaga
     1381 tccgcgccgg cgagatcgtc tgcctgctcg ggggcaacgc ctcgggaaaa tccacgacga
     1441 tgaagacggt cctcggcctc gtgcgtccgg cccgggggac cgtgctgtac cgtggggagc
     1501 cgatcaacac cctgcccacc tcggagcgcg tccggcgggg gatcgcaccg gtgccggagg
     1561 cccggcggct gtttcccaag atgaccgtgt acgagaacct cgagatggga gcgtacaccc
     1621 gggacaatct cgtggaaatc caggaagacg ttgaccgcgt ctacgaattg ttcccgcggg
     1681 tcaaggagcg gcgcaaccag ctcgccggga ccctgtccgg gggcgagcag cagatggtcg
     1741 cgatcggccg ggcgctcatg gcgcggccga acctcgtgtg catggacgag ccgtccatgg
     1801 ggctgtcgcc tgccttcg
//
LOCUS       DASQGH010000112         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3253, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000112 DASQGH010000000
VERSION     DASQGH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3253"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VEW91_02185"
     CDS             complement(<1..890)
                     /locus_tag="VEW91_02185"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HYM90426.1"
                     /translation="MAGQASAKYVAAILAIAILPALPGMRARAAQAHTVQLTLIGGKT
                     QAAGGYNFNGYADGQLVVTVPLGWTVTVQFENAASPPHSVAVLPYSDPQPAEGTRPAF
                     PGATTPNLQGGIPRGARATFSFVASRPGTYEFACGVAGHAIAGMWATLIVSPTATAAT
                     TQIRPPRSPAPPPSVASGTTAVAGPDLPGNVLIADAGNNRRIEVTPDKRIVWEFPRPG
                     DLGPGQTFVDPDDAFYTPGGRTIITNEEDNHTIALIDYATHKIVWEYGHPGQPGSGPG
                     YLDTPDDAYQLPDGRIVVAD"
     gene            complement(890..2122)
                     /locus_tag="VEW91_02190"
     CDS             complement(890..2122)
                     /locus_tag="VEW91_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HYM90427.1"
                     /translation="MHTGHRAPIIRRPAIYVERRPFIVIWEVTRACDLACAHCRAQAA
                     PLPHPDELTTEEARKLIDEIAAFAPPAPLFVLTGGDPMKRPDLLDLVAHAVHRRLPVA
                     LSPSATPLLSAAAVADIRAAGAVALSLSLDGASAGAHDAFRGFPGVFDRTLAAWSAAR
                     ACGLKVQINTTVTRANLVDLPGIAQLVRARGAMTWSVFFLVPVGRGVALPQLSPDECE
                     DVLHFLHDVGDALPVKTTEGHHYKRVILERAVLERRGIPLEDVVSLGPTYRSLRAALE
                     PWPAAVAGRRSPMDVNAGRGFVFVSHTGTVHPSGFLPLVAGSVRRQSLGEIYRQSPLF
                     RALRDPARLRGRCGECEFATVCGGSRSRAFAVSGDPLAEDPLCAYLPGTFPFPDDIAE
                     VLNGAGPRPARAGAGGEP"
BASE COUNT          330 a          722 c          775 g          309 t
ORIGIN      
        1 atgtctgcca caacgatgcg cccgtcgggg agctgatagg catcgtcggg cgtgtccagg
       61 tagccggggc cgctgccggg ctgccccggg tggccgtact cccagacgat cttatgcgtc
      121 gcatagtcga tgagcgcgat cgtgtggttg tcttcttcat tggtgatgat cgtccggccg
      181 ccgggcgtgt agaacgcatc gtcggggtcc acaaaggttt gcccggggcc caggtcgccc
      241 ggccggggga actcccagac gatccgcttg tccggcgtca cctcgatgcg gcggttgttg
      301 ccggcatctg cgatcagcac gttccctgga aggtccgggc ccgccacggc cgtggtaccc
      361 gaggcgacgc tcggaggcgg cgccggcgag cgggggggac ggatctgggt cgtcgcggcc
      421 gtggcggtgg gcgatacgat gagggtcgcc cacatgccgg cgatcgcgtg cccggccacg
      481 ccgcaggcga actcgtaggt gcccggcctg ctggcgacaa acgagaacgt ggccctggcc
      541 ccccgcggga tcccgccctg gaggttcggt gtggtggccc cgggaaaggc cggacgtgtc
      601 ccctcggcgg gctgcggatc gctgtacggc agcaccgcaa cactgtgggg aggcgacgcc
      661 gcgttctcga actggaccgt caccgtccaa cccaggggga cggtgacgac cagctggccg
      721 tccgcatagc cgttaaaatt gtatcccccg gcggcctgcg tcttccctcc gatcaatgtc
      781 agctgcacgg tatgggcctg cgcggcacgg gcccgcattc cggggagcgc gggcaggatg
      841 gcaatggcga gaattgccgc aacgtatttg gccgaggcct gcccggccat cagggctcac
      901 cgccggcccc tgcccgcgcc gggcgagggc ccgctccgtt gagcacctcg gcgatatcgt
      961 ccggaaacgg gaacgtcccc gggaggtagg cgcacagggg gtcttccgcc aggggatctc
     1021 cgctcaccgc gaacgcccgg gaccgcgacc ctccgcacac ggtcgcgaac tcgcactccc
     1081 cgcaccgtcc gcgtaggcgc gccgggtccc gcagcgcccg gaacagcgga ctctgccggt
     1141 agatctctcc gagcgactgg cgacgaacgc tgcccgcgac gagggggaga aagccgctgg
     1201 ggtggacggt gccggtgtgg gagacgaaca cgaagccgcg gccggcgttc acgtccatcg
     1261 ggctgcggcg tccggctacc gcggcgggcc agggctccag ggccgcccgg agcgaccggt
     1321 acgtggggcc gagcgacacg acatcttcaa gaggaatgcc gcgtcgctcg aggaccgcgc
     1381 gctcgaggat gacgcgtttg tagtgatgcc cttcggtcgt cttcaccggc agcgcgtccc
     1441 cgacatcgtg aaggaagtgc agcacgtcct cgcactcgtc gggagagagc tggggcagtg
     1501 ccaccccgcg tcccaccggg acgaggaaga aaacgctcca ggtcatcgct ccccgcgcgc
     1561 gcacgagttg ggcgatcccg gggaggtcta cgaggtttgc ccgggtcacc gtggtgttga
     1621 tctggacttt gaggccgcac gcccgggcgg cgctccaggc ggcaagggtc cggtcgaaga
     1681 ctccgggaaa gccacggaac gcatcatgcg cgccggcact cgcgccgtcc aggctcaggg
     1741 aaagggccac cgccccggcg gcccggatgt cagcgaccgc cgccgctgag aggagaggcg
     1801 tcgccgacgg agagagcgca accggaagcc gccggtgcac cgcgtgcgcc accagatcca
     1861 gcagatcggg ccgtttcatc gggtcgccgc cggtgaggac gaacagcggc gctggcggcg
     1921 caaacgctgc gatctcgtcg atgagcttcc gcgcttcctc ggttgtcagc tcatcaggat
     1981 gaggcagcgg cgccgcctgc gcccggcagt gggcgcaggc gaggtcgcac gcgcgggtca
     2041 cttcccagat cacgataaac ggccggcgct ccacatagat ggcagggcgc ctgatgatcg
     2101 gcgccctgtg gcccgtgtgc aatggctata ccctcc
//
LOCUS       DASQGH010000113         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37346, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000113 DASQGH010000000
VERSION     DASQGH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37346"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VEW91_02195"
     CDS             complement(<1..1031)
                     /locus_tag="VEW91_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYM90428.1"
                     /translation="MEDLLFGTLSELGSGLRRRRFSSVDLARMALDRLESLGPRYNAV
                     ASLLPERALREARAADRRLKDGRALGALVGIPYGAKDLLAARGAATTWGTRPYARQAF
                     DYDAAVIERLRGAGAVLVAKLAMVALAGGGGYRYPSASLQGPARNPWNPEHWAGGSSS
                     GTGVAVAAGLVPFGIGSETSGSIFSPSANCGITGLRPTYGLVSRFGAMALAWTMDKLG
                     PMCRSAEDCGLVLEAIAGGDPRDPGSAGRAFRLGRGRGPRLSGLRVGCLTDDFEAVHP
                     SMGSAMRGAVAAFRSLGVRWKRIAVPPLAIDSVTRTIIRAEGSAVFEDLIRSGRVALL
                     EDRRQEIGLR"
     gene            complement(1269..2267)
                     /locus_tag="VEW91_02200"
     CDS             complement(1269..2267)
                     /locus_tag="VEW91_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYM90429.1"
                     /translation="MPKIILLNRMLHQGGQEWLAARADVEVVPPDAPGERIAQAVASA
                     DAVLVRLPIRLTRELVAAAPRLRVIGIEGAGFDHIDVAAATEAGIPVVNNVGVGATPV
                     AEHAVGLMLALARRIAKGNAGLRHEGWACRDAFLGGEMGTELSGKTVGIVGFGAIGRQ
                     VARICLAAFENRILAYDPLLDDGLFHAMHVDRRRRLRELLPEADFVTIHTPLNAETRH
                     VIGAAELGLMKPTAYLINCARGPVVDEAALYAALHEGRIAGAGIDVFDPEPPASTNPL
                     FGLPNVIVTPHIAGVSQETSRRLSLAAAEQVMQVLADERPPRLVNPEVWARRRRPT"
     gene            complement(2260..2853)
                     /locus_tag="VEW91_02205"
     CDS             complement(2260..2853)
                     /locus_tag="VEW91_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HYM90430.1"
                     /translation="MWRSSGGKIILASASPRRVELLASAGIEVDAVPSRLPEEFRPGE
                     AVADHVRRLSEAKARGVAAATAGRFFIGADTIVVRGDKVMGKPRDRAEAERMLRALSG
                     GSHKVITGYAVYDAAGDRVRVGAATTLVYFKALSPAEIAAYVAAESPYDKAGAYAIQG
                     RAAYMVHRIEGSYTNVVGLPLCEIVEVLVEMGAIVHA"
     gene            complement(2865..3755)
                     /locus_tag="VEW91_02210"
     CDS             complement(2865..3755)
                     /locus_tag="VEW91_02210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYM90431.1"
                     /translation="MEYGITLPGAGPLATSDALMAVATLAESLGFASLWVTDHIAIPH
                     ESRSAYPYSADRKAPWPPNIPYLDALTALGWVGAVTRTIRLGTSVLILPLRPPLAVAK
                     AVGTLDYLTGGRILLGVGAGWLKEEFDLLGQPFANRGRRMKEAIRILKACWAGDPVEF
                     QGDLYRLAPFGMDPKPAQGARLPILGGGESDAALRRVAEVCDGWHPLNLTPDQVAERL
                     VRLRGFVAAAGRAMADLLLTARPGRNVPVTPDLARRYEALGVRMLVADVDYRSLTLPE
                     ALAHIARLAKTLRLSAAVAS"
BASE COUNT          598 a         1477 c         1380 g          538 t
ORIGIN      
        1 cgcagcccga tctcctggcg ccggtcttca aggagggcca cgcgcccgct ccggatcagg
       61 tcctcgaaga ccgccgatcc ctcggcccgg atgatcgtcc tggtcaccga atcgatcgca
      121 agcgggggaa ccgcgatccg cttccaccgg acgccgaggc tccgaaacgc cgccaccgcc
      181 ccccgcatcg cggaccccat cgagggatgc acggcctcga agtcgtcggt caggcacccc
      241 acgcgcagcc cggagaggcg tggccctcgt ccccgtccca gccgaaacgc gcggccggcg
      301 gatcccgggt cgcgagggtc gcctccggcg atcgcttcga gcacgaggcc gcagtcctcc
      361 gcgctccggc acatcggtcc gagcttgtcc atggtccacg cgagcgccat ggccccgaag
      421 cggctcacca gcccgtaggt ggggcggagc ccggtgatcc cgcagttggc cgacgggctg
      481 aagatggacc ccgaggtctc cgagccgatc ccgaacggca cgagccccgc cgccaccgcc
      541 acccccgtgc cgctcgagga gccgccggcc cagtgctcgg ggttccaggg gttcctggcg
      601 ggcccttgaa gggaggccga ggggtagcgg tacccgcccc cgccggcgag cgccaccatc
      661 gcgagcttgg cgaccagcac cgcccccgcc ccgcgcagcc tctcgatcac cgccgcatcg
      721 tagtcgaagg cctggcgggc gtacggccgg gttccccacg tcgtcgccgc ccccctggcc
      781 gccaggagat ccttcgcgcc gtacgggatg cccacgagcg cccccagggc acggccgtcc
      841 ttgagccgcc ggtccgccgc ccgggcctcg cgcagcgccc gctcgggaag gagagaggcc
      901 accgcgttgt accgggggcc gagggactcg agccggtcga gcgccatccg ggccaggtcc
      961 accgaggaaa accggcggcg cctgaggcct gagcccagtt cgctcagggt gccaaagagg
     1021 agatcttcca tggatcagag tttgaagacc ggggccggct cggtcgtgat cggcactgga
     1081 acgcgccgca tcgcctcgcc ggactcccgc tgcactttga ggacctgccg caactcccga
     1141 gccaccgcgc gccggtctcc ccgcccccac gtgcgcggtg ggcgggacga gcggggagcc
     1201 ttcggcaaag ttcgagaccg cctgttcatg ctccgtcagt accacacgga gcggggctat
     1261 tcctcgacct aggtggggcg gcggcggcga gcccacacct ccgggttgac gagccgaggc
     1321 ggccgctcat ccgccagaac ctgcatcacc tgctccgccg ccgcgaggga cagccgccgg
     1381 ctcgtctcct ggctcacccc ggcgatgtgc ggggtcacga tgacgttcgg caatccgaag
     1441 agggggttgg tggaggccgg gggctcgggg tcgaagacat cgatccccgc cccggcgatc
     1501 cgcccctcgt ggagcgccgc gtagagggcg gcttcgtcca caacaggccc ccgcgcgcag
     1561 ttgatcaggt aggcggtcgg cttcatcagg cccaactccg cggcgccgat cacgtggcgc
     1621 gtctccgcgt tgagcggggt gtggattgtc acgaagtcgg cctcggggag cagctcccgc
     1681 aaccggcgcc ggcgatcgac gtgcatggcg tgaaagagcc cgtcgtcgag gagcgggtcg
     1741 taggcgagga tgcggttctc gaaggccgcg aggcagatcc gggccacctg ccggccgatt
     1801 gcgccgaagc cgacgatccc cacggtcttg cccgagagtt cggtccccat ctccccgccg
     1861 aggaacgcgt cccggcaggc ccatccctcg tgccggaggc ccgcattgcc cttcgcgatg
     1921 cgcctggcga gggccagcat caggccaacg gcgtgctcgg ccaccggggt ggccccgacc
     1981 ccaacgttgt tgacgaccgg gatcccggct tcggtcgcgg cggccacatc gatgtggtcg
     2041 aaccccgccc cttcgatccc gatcacccgg aggcgcggag ctgcagccac cagttcccgc
     2101 gtcaggcgga tggggagccg gaccaggacg gcgtcggccg acgccaccgc ctgcgcgatg
     2161 cgctcccccg gcgcgtctgg gggaacgacc tccacgtcgg cgcgggccgc gagccactcc
     2221 tgacccccct ggtggagcat gcggttgagc aggatgatct taggcatgaa cgatcgcccc
     2281 catctcgacc aggacctcga cgatttcaca cagcgggagg cccaccacgt tggtatagga
     2341 gccttcgatc cggtggacca tgtaggcggc gcgtccctga atcgcatagg cgccggcttt
     2401 gtcgtacggg ctttccgccg cgacgtacgc cgcgatctcc gccgggctca gcgccttgaa
     2461 atagacgagg gtcgtcgcgg ccccgacccg gacccggtcg cccgcggcgt cgtagaccgc
     2521 gtacccggtg atcaccttgt gcgatcctcc ggaaagcgcc cggagcatcc gctcggcctc
     2581 ggctcggtcc cgcggcttgc ccatgacctt gtccccgcgc accacgatgg tgtcggcccc
     2641 gatgaagaac cgccccgccg tggcggcggc cactcccctg gccttagcct ccgacaggcg
     2701 ccggacgtgg tcggccacgg cctcccccgg ccgaaactcc tccggaagac ggctgggcac
     2761 cgcgtcgacc tcgatgcctg cggacgccag gagttcaacc ctccgcgggg acgcggacgc
     2821 gaggatgatc tttccgccgg aggagcgcca cagaggggcc gggctcacga agcgaccgcg
     2881 gcggagaggc gcagggtctt cgcgagccgc gcgatgtggg ccagcgcctc gggcaacgtg
     2941 aggcttcgat agtccacgtc ggcgacgagc atccggacgc cgagggcctc gtaccggcgt
     3001 gcgagatccg gcgtgaccgg cacgttccgg ccggggcgcg cggtcagcag caggtccgcc
     3061 atggcgcgcc ccgccgcagc cacaaacccc cggagccgga ccagccgctc ggccacctgg
     3121 tcgggcgtga ggttcaacgg atgccacccg tcgcacacct ccgccacccg gcgcagcgcc
     3181 gcgtcgctct cccccccgcc caggatgggc aatcgcgcgc cctgggcggg cttgggatcc
     3241 atcccgaacg gcgccagccg gtagaggtca ccctgaaact ccaccggatc gccggcccag
     3301 catgccttga ggatccgtat cgcttccttc atgcggcgcc cgcggtttgc gaacggctgg
     3361 cccaggagat cgaactcctc cttgagccac cccgccccca ccccgagcag gatccggccc
     3421 ccggtgaggt aatcgagggt tcccacggcc ttggccacgg ccaggggcgg ccgcagcggg
     3481 aggatgagga ccgaggtccc cagccggatc gtgcgggtca ccgccccgac ccagccgagc
     3541 gccgtgaggg cgtcgagata cggaatgttc ggcggccacg gtgccttccg atccgcgctg
     3601 tagggatacg cgctgcgcga ctcgtgggga atggcgatgt ggtcggtcac ccacaacgag
     3661 gcaaagccca gggactctgc gagggtcgca accgccatca gcgcgtcgga ggttgcgagc
     3721 ggacctgcgc cgggaagcgt gatgccgtat tccattctcg tcgctccgcc tatggtaggc
     3781 ttgggacgaa ccgggtgtca aggcggggga tccgatggca gcggagggcg cacagacgca
     3841 gcagaccgaa ggcgcattcg acacggtccg tcgcgcgtac gggatcgcgg tgcgggacct
     3901 gcgggcctgc tacaaccccg acggcatcgt cgccgggcgc ctgcacttca acgcctactg
     3961 ggcgcgggac ggattctggg cgctgttcgg cgc
//
LOCUS       DASQGH010000114         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45793, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000114 DASQGH010000000
VERSION     DASQGH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45793"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VEW91_02215"
     CDS             <1..200
                     /locus_tag="VEW91_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HYM90432.1"
                     /translation="GVEVAGGMIYCTHQPCLTCAKMIVNAGLTRIVYESPYPDPVAEQ
                     LLRDAAIDLARVPAGESVRQG"
     gene            301..1101
                     /locus_tag="VEW91_02220"
     CDS             301..1101
                     /locus_tag="VEW91_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYM90433.1"
                     /translation="MERISLIAVLRPETGKNAVKRVRKAGLVPAVLYGRNRAPVPLAI
                     DRRALLGALHTEAGRNVLIDLQVKRNGEEVSDTVMIAEIQHDHIRRQVLHVDLHQISL
                     SEKIEVRIPIVLAGVPEGVADGGGILEQHLRELVVRCLPTAIPEHVTANVQGLRLGAS
                     LHVRDLAPAEGVEIVTPPEGVIAAVVAPKEEEVAAPVEAAAAPAEPELVGKEAAPAPE
                     GEAKAEPGARTAAKPAAGSPSPAGDTRAGGAAGRPRGGDKPARAEKKE"
     gene            1150..1773
                     /gene="pth"
                     /locus_tag="VEW91_02225"
     CDS             1150..1773
                     /gene="pth"
                     /locus_tag="VEW91_02225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYM90434.1"
                     /translation="MEKWRALSAFIMMIVVGLGNPGRRYKGTRHNVGQEVVHHLAARL
                     RVRLQDDGWARTGKGRVDSAVVLLAIPETYMNVTGQAVRDLLHRRRRGPEDLLVVHDE
                     MDLSLGHLRLRRGNGPGGHNGVRSIIEELGTGMFPRLRLGIGRPPAGEDPAEFVLGRF
                     TPEERRTIDEAVALAVEAVTVAATQGLAVAMNRYNRRAASQGLVESR"
     gene            1770..2471
                     /locus_tag="VEW91_02230"
     CDS             1770..2471
                     /locus_tag="VEW91_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYM90435.1"
                     /translation="MSSVREARPGVAALRRWVADETWLKRLLTAQRWMSRAYFEARYV
                     TPDPWRLATSPYELERAGASLRMLGGRHYGSALEVGCGEGCFTGRLLDRCDHVVAVDF
                     SALALRRARRRFAGDPRVEVKQLDVRAGDLDRTFDLVVCAELFYYMNRAELEKVGARV
                     VRWIAPGGDLCLVHGTSVHDAAPGGARAGAPALMSARMIHDRFRRMPGLAVLRDLPLP
                     RYRLTLLRRTEAGGG"
     gene            2464..2604
                     /locus_tag="VEW91_02235"
     CDS             2464..2604
                     /locus_tag="VEW91_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90436.1"
                     /translation="MADQTATAPAPTARSLRRDVPVWGSFMWGFADVGAEALLGKAVP
                     TR"
     gene            2740..3330
                     /locus_tag="VEW91_02240"
     CDS             2740..3330
                     /locus_tag="VEW91_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM90437.1"
                     /translation="MTEATPDAAPTGTAHRLAAFEELVQRQQTQIYRVAYRLTGNHDD
                     AEDLAQEAIVEAFKAFDRYQPGTYFDRWLYRIMSRTYIDIVRRRNRRPVVSLDAPVGT
                     EGDPLVTLVGDTSQDPQQMTETMDLDGAIQAALDGLPQEFRTAVVLADIEGLSYDEVA
                     GALRCPVGTVRSRLHRARQMLREALGPRLGARRDDR"
     gene            3327..3821
                     /locus_tag="VEW91_02245"
     CDS             3327..3821
                     /locus_tag="VEW91_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYM90438.1"
                     /translation="MSGHVSDQLSAYIDGALGVQDVERVKAHLDICPRCQQEYRGVQE
                     VQRLLRALPDPTPPAGFLDRVHWRLQREAAGHPRPGLVARVLGVFTPRPLRLALAASA
                     LVLILGLPWAWMTGQFGPRQAPLDTDAYLRHYFVLSADRSLADEATTTFVSFDLGLSE
                     QPTR"
BASE COUNT          560 a         1312 c         1504 g          599 t
ORIGIN      
        1 atggggtgga ggtcgccggc gggatgatct actgcacgca ccaaccgtgt ctgacctgcg
       61 ccaagatgat cgtcaacgcg ggactcaccc gcattgtgta cgagtcgccg taccccgacc
      121 cggtggccga gcagctcctt cgggatgcgg cgatcgacct ggcgcgcgtc ccggcgggcg
      181 aatcggtgcg ccaggggtga ccctcggcgc ggagcgtgct ataatgaccg gtcgtggact
      241 tgtgcgggcg ccgcgggccg ccccaagtgc ggcggcgggg gacggaggag ggttacgggg
      301 atggagcgca tcagcctgat cgcggtgctg cggcccgaga ccgggaagaa cgcggtcaag
      361 cgggtcagga aggcgggcct cgtgccggcg gtgctctacg ggcggaaccg cgcgccggtg
      421 ccgttggcga ttgaccggcg ggcgctgctg ggcgcgctcc acaccgaggc gggccggaac
      481 gtgctcatcg acctgcaggt gaaacgcaac ggcgaagagg tcagcgacac cgtcatgatc
      541 gcggagatcc agcacgacca catcaggcgg caggtgctcc acgtggacct gcaccagatc
      601 agcctctccg aaaagatcga ggtgcgcatc cccatcgtgc tcgccggcgt tcccgaaggc
      661 gtcgccgatg gcggtggcat cctcgaacag cacctgcgcg aactggtcgt ccgctgtctg
      721 cccacggcga ttcccgaaca cgtgacggcc aacgtccagg gcctgcgcct gggagcgtcc
      781 ctccacgtgc gggatctcgc ccctgcggaa ggggtcgaga tcgtcacccc ccccgaaggg
      841 gtgatcgccg ccgtcgtggc gccgaaggaa gaagaggtcg cggccccggt cgaggcggcg
      901 gcagcgccgg ccgagccgga gctggtgggg aaggaggccg ccccggcccc cgagggggaa
      961 gcgaaggccg aaccgggggc ccggaccgcg gccaagcccg ctgccggctc accttcccct
     1021 gctggggata cccgtgcggg tggcgcggcc ggcaggccgc gcgggggaga caagccggcc
     1081 agggcagaga agaaggagta gttcatgccg gtggccggct agggccgagg ggacagtgcg
     1141 cggaagggcg tggagaagtg gcgcgccctt tctgctttca tcatgatgat cgtggtcggt
     1201 cttgggaatc ccggccggcg ctacaaggga acccggcaca acgtgggcca ggaggtggtc
     1261 caccacctgg cggcgcgact ccgggtcagg ctccaagacg acggctgggc caggacgggg
     1321 aaggggcggg tggatagcgc cgtcgtgctc ctggcgattc cagagaccta catgaacgtc
     1381 accggccagg cggtcaggga cctcctgcat cgccgccggc ggggtcccga agatctcctc
     1441 gtcgtgcacg acgagatgga tctgtcgttg ggccacctca ggctccggcg gggcaatgga
     1501 cccggcgggc acaacggggt gcggtcgatc atcgaggagc tcgggaccgg gatgttcccc
     1561 cggctccggc tcgggatcgg gcggccgccc gcgggcgagg atcccgccga gttcgtgctg
     1621 ggccggttca cccctgagga gcggcgcacc atcgacgagg cggtggcgct ggccgtcgaa
     1681 gcggtgacgg tcgcggcgac ccaggggctc gctgtcgcga tgaaccggta caatcgccgg
     1741 gccgcttcgc agggcctcgt ggagtcgcgg tgagcagcgt ccgggaagcg cgtccggggg
     1801 tggcggcgct ccggcggtgg gtggccgatg agacctggct caagcggctg ctcacggcgc
     1861 agcgctggat gagccgggca tacttcgagg cgcggtacgt cacgccggat ccctggcgcc
     1921 tcgcaacctc gccgtacgag ctggagcgtg ccggggcgtc gttacggatg ctcgggggcc
     1981 ggcactacgg gagcgccctg gaggtcggct gcggcgaagg gtgcttcacc ggccgtctgc
     2041 tggatcggtg cgaccacgtc gtggccgtcg acttctccgc tctcgcgctt cggcgggccc
     2101 gccggcggtt cgccggggat ccccgggtcg aggtgaagca gctggacgtt cgcgccgggg
     2161 atctggatcg gacgttcgac ctcgtggtct gcgcggagct cttctactat atgaaccgcg
     2221 ctgagctcga gaaggtcggc gcgcgcgtgg ttcgatggat cgcgcccggc ggcgacctct
     2281 gcctggtcca cgggacgtcg gtgcacgacg ccgcccccgg gggcgcgcga gcgggagccc
     2341 ccgcgctgat gagcgcccgc atgatccatg accggttccg ccggatgccc ggtctggcgg
     2401 tcctccgtga tctccccctg ccccgctacc ggctgaccct gctccggcgg accgaggccg
     2461 gcggtggctg accagaccgc aaccgccccg gcccccacgg ctcggagcct tcgccgcgac
     2521 gtgccggtgt ggggctcgtt catgtggggg ttcgccgatg tcggcgccga ggccctgctg
     2581 ggcaaggctg tccccacgcg ctgagcctcg cgggatcccc gccccgaacc ttcgctggcg
     2641 gggaactttt cgccggccgg ccgcgcttgg tatggtaagc agcggactgg gggcccggcg
     2701 atggcagaag ccgtatcggt cgtgggaggc ctggcgggca tgacggaggc gacgccggac
     2761 gccgccccga caggcacggc gcatcgactg gcagccttcg aggagctggt gcaacgccaa
     2821 cagacccaga tctaccgggt cgcctatcgg ttgaccggca accatgacga cgccgaggac
     2881 ctcgctcagg aggcgatcgt cgaggcgttc aaggcgtttg accgctacca gccggggacc
     2941 tatttcgacc ggtggttgta ccggatcatg agccggacct acatcgatat cgtgcgccgg
     3001 cggaaccgcc ggccggtcgt ctcgctggac gcgccggtgg ggaccgaggg ggaccccctg
     3061 gtcacgttgg tgggagacac cagccaggac ccgcagcaga tgaccgagac gatggatctg
     3121 gacggcgcca ttcaggcggc gctggacggg ctcccccagg agtttcgcac ggcggtggtt
     3181 cttgccgaca tcgaaggcct gtcgtatgat gaggtcgcag gtgccctgcg gtgtccggtc
     3241 gggacggtcc ggtcaaggtt gcatcgggcc cggcagatgc tccgcgaggc gctcgggcca
     3301 cgcttgggcg caaggaggga tgaccgatga gcgggcacgt ttccgaccag ctctcggcgt
     3361 atattgacgg cgccctcggc gtccaggacg ttgagcgtgt gaaggcccat ctggacatct
     3421 gcccgaggtg ccagcaggag taccgagggg tgcaggaggt ccagcgcctc cttcgggcgc
     3481 tccccgatcc gaccccgccc gcggggttcc tcgatcgggt gcactggcgg ttgcagcggg
     3541 aggccgcggg tcatcctcgt ccgggcctgg tcgcgagggt gctgggcgtg ttcacgccga
     3601 ggccgctccg gcttgcgctg gcggcgagcg cgctggtgct gatcctgggg ctcccctggg
     3661 cgtggatgac cgggcagttt gggccccgtc aggcgccgct cgacaccgat gcgtatctgc
     3721 gccactattt cgtcctctcc gccgaccgat cgcttgccga tgaagcgacc accacgtttg
     3781 tctccttcga tctgggcctc tcggaacagc cgacccggta gcatgccttc tcggattctc
     3841 cgccaggcgg ctgccctggc ggcgcttgcg ctggggacga gtgtcctcgc gtcccccggg
     3901 tggtcccagc ccgggggcgc cgaggccccg ctgtcctggc tgcggtcggg cgcggtcgct
     3961 ccgcgacagg tcagc
//
LOCUS       DASQGH010000115         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_431, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000115 DASQGH010000000
VERSION     DASQGH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..705)
                     /locus_tag="VEW91_02250"
     CDS             complement(139..705)
                     /locus_tag="VEW91_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HYM90439.1"
                     /translation="MATRDLTAIALFAALTAVCAQASVVLPALSSVPVTLQVFAVLAS
                     GAVLGARRGFLSQVVYLLLGAAGFPVFARGHGGAQWLVGPTGGYLWAFPIAALVAGWG
                     WELWPGAGAGRVRFRILGAFAGLLCIYALGVAGLVATGTVPTLARAIRVGVIPFLLLD
                     LMKAALAAVIADRVRSAVPVLRAAHDRS"
     gene            complement(705..2153)
                     /locus_tag="VEW91_02255"
     CDS             complement(705..2153)
                     /locus_tag="VEW91_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYM90440.1"
                     /translation="MGTRLLASILLMVFTVASSALPARAQPVGPSAPPPPAQILGPIP
                     AIPQVPTLPPRPLTIGDALTTGLQNSFQTRLSAFGVVIARAQLQQAVAQEAVTLSGVA
                     SFTANSVSGGAPLTGTISIPGAVPAVTNQPFTASGLGGFGTTPTTTVFALALKYPLYT
                     GGALEAQVSIAQANLALAEAQFTTAAQAVVLSVRQAYYTARATQANVDAAQRTVDASR
                     ENVRVTEARVRVGSSPQFDLLQAQVQLAASEQALTTARTGHATGQQALAAALVAPLST
                     TFALEAPTSLPRVPEDVNALIQQALQHRPEIAAALASERAAQAAIDLAASGLAPNITV
                     TGGPQIQTSNPTSQDTVNFTGAIVLTLAILDGGLTDGKVAAARASLAQSKVSEEQTRQ
                     QVELDVRNAYLLLGGAADVLRSARVAQAAATEALRIANVRFQAGVGTQLEVVTAVQNL
                     ATADSNVIQSALQYFVAAAQLDRAVGIQVEIF"
     gene            complement(2216..>2666)
                     /locus_tag="VEW91_02260"
     CDS             complement(2216..>2666)
                     /locus_tag="VEW91_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYM90441.1"
                     /translation="QLGILVLFGVVKKNAILQIDRANHLRVLGLDRDAAIVAASRDRL
                     RPILMTTIAFVAGMIPLAISSGVGAATNKAMSSVIIGGQVLSLLLTLVAIPVMYSLFD
                     DLGKVRVPAWRGALSWAGDRPAVPRWGRVNGETPTLRPGTSSSGDQR"
BASE COUNT          413 a          986 c          917 g          350 t
ORIGIN      
        1 gggatctgcc ccgcggcgaa gaccagcccg ccaccgacaa ccgcctggga gtacggaccg
       61 atcgccggtg gggcctcctc ggtcttgatc acacgcttgg gcatgccgcc gtcacctctt
      121 tctccccgaa cggggccctc atgagcggtc atgggccgcc cggaggaccg gcacggccga
      181 tcggacgcgg tccgcgatca cggccgccag ggcggctttc atcagatcca ggaggaggaa
      241 cgggatgaca cccacccgga tcgcccgcgc cagcgtgggc acggtcccgg tcgccaccag
      301 gccggccacc ccgagcgcgt agatgcacaa caggccagcg aaggcaccca ggatccggaa
      361 gcgcacccgc ccggcccccg cgccgggcca gagttcccaa ccccacccgg cgacgagcgc
      421 ggcgatcggg aacgcccaga ggtatccccc ggtcggcccg acgagccact gcgccccccc
      481 gtgccctcgg gcgaacacgg gaaatccggc cgcccccaag aggagataga cgacctggct
      541 caggaacccc cggcgcgctc ccaggacggc cccggacgcc agcacggcga agacctgcag
      601 cgtcaccggc acactcgaca gcgcgggcaa gacgacgctg gcctgggcgc acacggcggt
      661 cagcgccgca aagagcgcga ttgcggtcag gtcccgcgtc gccatcaaaa gatctccacc
      721 tggattccta ccgcgcggtc gagctgcgcc gccgcgacga agtactggag cgccgattgg
      781 atgacgttgc tgtcggccgt ggccaagttc tgcaccgccg tgaccacctc gagctgggtg
      841 ccgacgccgg cctggaaccg gacgttggcg atccgcaacg cttcggtcgc cgcggcctgg
      901 gccacgcgcg ccgaccgcag cacgtccgcg gcgccgccga gcaacaggta ggcgttccga
      961 acatcgagct ccacctgctg gcgcgtctgc tcctcgctca ccttcgattg cgcgaggctc
     1021 gcccgcgccg ccgcgacctt cccgtccgtc agccccccgt cgaggatcgc gagggtgagc
     1081 acgatggcgc cggtaaagtt caccgtgtct tggcttgtcg ggttgctcgt ctggatctgc
     1141 ggcccgccgg tgacggtaat gttcggcgcg aggcccgagg ccgccaggtc gatcgcggcc
     1201 tgcgcggccc gctcgctcgc gagcgccgcg gcgatctccg gccggtgctg cagcgcctgc
     1261 tggatcagcg cgttcacgtc ctcgggcacc cggggcaggc tggtcggggc ctcgagcgcg
     1321 aacgtggtcg acagcggggc gaccagggcg gccgccaggg cctgctggcc cgtcgcgtgc
     1381 cccgtccgcg ccgtcgtcag ggcctgctcc gacgcggcca gctgcacctg cgcctggagc
     1441 aggtcgaact ggggggaact gcccacccgc acgcgggcct ccgtcacccg cacgttttcc
     1501 ctcgaggcgt ccaccgtgcg ctgcgccgcg tcgacattgg cctgggtggc ccgcgccgtg
     1561 taatatgcct ggcggacgga cagcaccacc gcctgcgcgg ccgtggtgaa ctgggcctcc
     1621 gcgagcgcga ggttcgcctg ggcaatcgag acctgcgcct cgagcgctcc acccgtgtac
     1681 agggggtact tgagggccag cgcaaacact gtggtcgtgg gggtcgtccc gaacccgccg
     1741 agccccgacg ccgtgaacgg ttggttggta accgccggca ccgccccggg gatcgagatg
     1801 gtgccggtca ggggggcgcc gcccgatacg gagttcgcgg tgaagctggc tacgccgctg
     1861 agcgtcaccg cctcctgggc caccgcttgc tgcagttgcg cgcgggcgat cacgacccca
     1921 aacgctgaca gccgggtttg aaagctgttc tgcagccccg tggtgagcgc atcgcctatc
     1981 gtcagcggac gcggcggcag cgtcgggacc tgggggatgg ccgggatcgg ccccaggatc
     2041 tgcgcgggag ggggcggggc ggacggcccg accggttggg cgcgcgccgg gagagccgaa
     2101 gacgccaccg tgaacaccat caataggatg gaagccagca gccgggtccc catgcaccac
     2161 cctcctcgac taacgttcaa cgagtcgcaa ccagcgccgc cctcggggcg ccccatcacc
     2221 gttgatcccc gctcgaagag gtgccgggac gcagcgtggg ggtttccccg ttcacccgcc
     2281 cccaccgagg aacggcgggc cggtcccccg cccaagacaa cgcgccccgc caggcgggga
     2341 cccgcacctt ccccaggtca tcgaacaggg agtacatcac cgggatcgcg accagcgtca
     2401 gcagcagcga caggacctgg ccgccgatga tcaccgagct catcgccttg ttggtcgcgg
     2461 cgcccacccc gctcgagatc gcgagcggga tcatcccggc cacaaatgcg atcgtggtca
     2521 tcaggatcgg gcgcaggcga tcgcggctcg ccgcgacgat ggcggcgtcg cgatcgaggc
     2581 ccagcacccg caggtggttc gcccggtcga tctgcagaat cgcgttcttc ttgacgacgc
     2641 cgaacagcac gaggattccc agctgg
//
LOCUS       DASQGH010000116         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6198, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000116 DASQGH010000000
VERSION     DASQGH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6198"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..705
                     /locus_tag="VEW91_02265"
     CDS             64..705
                     /locus_tag="VEW91_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYM90442.1"
                     /translation="MSSTRREILSLIKRRGPMTGQELCRTLEIPPMGVRQHLAILERD
                     GYIHSDGTRRGQGRPSRLYGITPEGDKLFPRTYEQLAHTLLDDLRAVGGEPKVDELFE
                     HRRRRLLEQYRGRMAGKEFREKVAVLAQAREEEGYLAEHAQLDRERFVLIEHNCPIRA
                     VAEAYPQICRCEMTLFADALGTEVVRTDHILAGAPHCRYIIARPREAGGKPPA"
     gene            810..1490
                     /locus_tag="VEW91_02270"
     CDS             810..1490
                     /locus_tag="VEW91_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYM90443.1"
                     /translation="MATLTYYGHATWGLETHGTTILIDPFFTGNPQTSVTAADVWADF
                     IILTHAHGDHYGDTVEIAKRTGATLISNYEIVSYCQKQGVANAHPLHIGGGYAFPFGR
                     VKLTVAHHGSSFPDGTYGGSPAGVLLEIEGKRLYDAGDTALFSDMSLIAHPALDVALL
                     PIGDNFTMGPEDAAAAAKLLRARTVIPQHYNTWPVITQDPEAFKRRVEAATESKVIVL
                     KSGGTCPV"
     gene            complement(1494..>1764)
                     /locus_tag="VEW91_02275"
     CDS             complement(1494..>1764)
                     /locus_tag="VEW91_02275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYM90444.1"
                     /translation="VIGPGEELFWRGLVQWGLGRRLGPGWGWAMATTLYGGAHLAAAN
                     PMLVVAALVAGGFWGLLYLRTGRIAPLVVSHILWDLTVFLILPFA"
BASE COUNT          305 a          629 c          583 g          247 t
ORIGIN      
        1 ttcgcacctt ggggctctgg cagccaccct tgcctttggt agaattgtgg ggaggggtgc
       61 agcatgtcgt cgacccgccg ggaaattctc tcgttgatca aacgccgcgg acccatgacc
      121 gggcaggaac tgtgccggac cctcgagatc cccccgatgg gcgtgcgcca gcatctggcg
      181 attcttgagc gcgacggtta tatccattcg gacgggaccc gccgcggcca gggccgaccg
      241 agccgcctct atgggatcac ccccgagggc gacaaactgt tcccccggac gtacgagcag
      301 ctcgcccaca cgctcctcga cgacctgcgg gccgtcgggg gggaaccgaa ggtcgacgaa
      361 ctcttcgagc accggcggag gcgcctgctg gaacagtacc ggggccggat ggcggggaag
      421 gagttccgcg agaaggtggc ggtgctggcg caggcgcgcg aggaggaagg ctacctcgcc
      481 gagcacgccc agctcgaccg ggagcggttc gtcctcatcg agcacaactg cccgatccgc
      541 gcggtggccg aagcgtatcc ccagatctgc cggtgcgaga tgacgctgtt tgcggatgcg
      601 ctgggcaccg aggtggtccg caccgatcat atcctggccg gcgcgcccca ctgccggtac
      661 atcatcgccc gaccccgcga ggccggcggg aagccgccgg cgtaaccccc cgaagctcgg
      721 cggcccggga cgcgcctcgc aggaattcgc ctgcgctcta gcggatcaac ctcccgcgcc
      781 gcgagaccag gcggcaggag ggaacggcga tggcgacgct cacctactac gggcatgcca
      841 cctgggggct ggagacccac ggcaccacga tcttgatcga tcccttcttc accgggaacc
      901 cccagacgtc ggtcacggcc gccgatgtct gggccgactt catcatcctg acccatgccc
      961 acggggatca ctatggtgac accgtggaga tcgccaagcg cacgggcgcc accctgatct
     1021 ccaactacga gatcgtcagc tactgccaga agcagggggt ggcgaatgcc catcccctcc
     1081 atatcggggg cgggtacgcg ttcccgttcg gccgggtcaa gctcacggtc gcgcaccatg
     1141 gctcctcgtt ccccgacggc acctatggcg gatcccccgc cggggtgctg ctcgagatcg
     1201 aaggcaagcg gctgtacgac gccggggaca cggcgctgtt ctccgacatg agcctcatcg
     1261 cccaccccgc cctcgacgtg gccctcctgc cgatcggcga caatttcacg atggggccgg
     1321 aggatgctgc cgcggcggcc aaactgctgc gcgcccgcac ggtcatcccg cagcactaca
     1381 acacctggcc ggtgatcacg caggacccgg aggcgttcaa gcgccgggtc gaggcggcga
     1441 ccgagtccaa ggtgatcgtc ctcaagtccg ggggcacctg cccggtgtga gcctcaggca
     1501 aacggcagga tgaggaacac cgtgaggtcc cagaggatgt gggacaccac cagcggcgcg
     1561 atgcgtccgg tgcggaggta gaggagcccc cagaaccctc cggcgaccag cgcggcgacc
     1621 acaagcatcg ggttggccgc ggcgaggtgc gccccgccgt acagggtcgt ggccatcgcc
     1681 cagccccatc ccgggccgag ccgccgcccg agcccccact ggaccaaccc ccgccagaac
     1741 agttcctccc ccggtccgat cacg
//
LOCUS       DASQGH010000117         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54187, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000117 DASQGH010000000
VERSION     DASQGH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54187"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VEW91_02280"
     CDS             <1..305
                     /locus_tag="VEW91_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYM90445.1"
                     /translation="PPSAARPAVLLLDARDTAAVAVTPIQPGAAVEVRRGSGTVRVVA
                     ETLIPFGHKIAVASMGAGDPVVKYGEVIGYATTPIRPGQHVHVHNVRSDRAKLTDG"
     gene            298..1290
                     /locus_tag="VEW91_02285"
     CDS             298..1290
                     /locus_tag="VEW91_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYM90446.1"
                     /translation="MADSPLAPLRGWAHPAGTGIRHHVLVIPSVICAAQASLTMVEGV
                     AGAVAIEHQHGCSQLGGDAHLTEQVLVGLGTHPNVAGTLVVSLGCETVQGVGLHRSIV
                     GRGQRAEFVGIQQAGGTGAAVRAGREALHRLRHAAASDPEVEVRWVDLRIGIEAAWLG
                     VAPAQARVVSALVDRLCRAGAAVIQAAPVGDGDAGAWWERQTRPLPAPASRPAPLAYA
                     TRPAGRGLFLMEAPLSRIAQKTGLAAAGAHVILSPLAEGMPAGCPVAPVVHVGIAPYA
                     RSFAGDVDVLLDEDPSAQAVEAILDAIGAACRTETALEALGAFEMAIHRIAPTM"
     gene            1296..>1913
                     /locus_tag="VEW91_02290"
     CDS             1296..>1913
                     /locus_tag="VEW91_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYM90447.1"
                     /translation="MDAVRLYRRPGARGWGTRNHVLVLPLVASASGIARALARGTGAT
                     WVEHDYEPTPDDLPRNRERIARTLVGFATNPNVGAVLLVADTAERGALLDPIRATGQR
                     ADLVAVAEAGGLRGAIEQGRARLAPLLAHAAAARREPAPVSALMLGTECGGSDALSGI
                     TANPALGVASDLLVDAGGTVILAETTELIGAEHLLARRAATPAVAA"
BASE COUNT          236 a          671 c          752 g          254 t
ORIGIN      
        1 accccccctc cgccgcccgt ccggctgtgc tgctgctgga tgcccgcgac accgcggccg
       61 tcgcggttac accgatccag cccggcgccg cggtcgaggt ccggcgcggg agcgggaccg
      121 tgcgggtggt ggccgagacg ctcatcccgt tcgggcacaa gatcgcggtc gcctcgatgg
      181 gggccggaga tccggtggtg aaatacggag aggtgatcgg gtacgcgacc acgccgatcc
      241 ggccggggca gcacgtgcac gtccacaacg tgcgcagcga ccgggcgaaa ttgaccgatg
      301 gctgattcgc cgctcgcccc gctgcgcgga tgggcgcatc cggcgggaac gggcatccgg
      361 catcatgtcc tggtgatccc ctcggtcatc tgcgcggctc aggcgagcct gacgatggtc
      421 gaaggggttg cgggcgcggt ggcgatcgag caccagcatg gatgctccca actcggaggg
      481 gacgcgcacc tcaccgagca ggtgctggtg gggctcggaa cccacccgaa cgtcgccggg
      541 acgctcgtcg tgagcctcgg ctgcgagacc gtgcaggggg tggggctcca ccgctcgatc
      601 gtgggtcggg ggcagcgtgc ggagtttgtg gggatccagc aggcgggcgg caccggggcc
      661 gctgtccggg ccggccgcga agccctgcac cggctccggc acgcggccgc atccgacccc
      721 gaggttgagg tccgctgggt ggacctgcgg atcgggatcg aggcggcgtg gctcggcgtg
      781 gcgccggcgc aggcccgggt cgtttccgcg ctggtcgacc ggctctgtcg cgccggggcc
      841 gcggtgatcc aggccgcgcc ggtgggagac ggtgatgcgg gcgcctggtg ggaacggcag
      901 acccggccgc tgcccgcgcc ggcctcgcgc ccggcgccgc tcgcgtacgc gacgcgcccc
      961 gcggggaggg gcctgttcct catggaggca cccctgtccc ggatcgcgca gaagaccggg
     1021 ctggccgcgg cgggggccca cgtgatcctc tcaccgcttg ccgaagggat gccggcaggg
     1081 tgcccggtcg ctccggtggt gcacgtcggg atcgcgccgt acgcgcggtc gtttgcgggg
     1141 gatgtcgacg tgctgctgga tgaggaccca tccgcgcagg cggtggaggc catcctcgat
     1201 gcgatcggcg cggcctgccg gacggagacg gcgctcgaag cgctgggggc gttcgagatg
     1261 gcaatccatc ggatcgcccc gacgatgtga ccgagatgga cgcggtgcgc ctttaccggc
     1321 ggccgggagc gagggggtgg ggcacccgca accacgtcct cgtcctgccg ctcgtggcct
     1381 cggcctccgg gatcgcgcgc gccctggccc gcgggacggg ggcgacctgg gtcgagcacg
     1441 actacgagcc aacccccgat gacctccccc ggaaccggga gcggatcgcc cggacgctcg
     1501 tggggtttgc gaccaacccc aacgtgggcg cggtcctcct cgtcgccgat acggcggagc
     1561 ggggcgcgct gctcgatccg attcgcgcca ccggccagcg tgccgatctc gtcgcggtcg
     1621 cggaagcggg gggcctccgc ggggcgatcg agcagggccg ggcgcggctc gcgccgctcc
     1681 tcgcgcatgc ggcggccgcc cgccgggagc ccgcgccggt gagcgcgttg atgctcggca
     1741 ccgagtgcgg gggatccgat gcgctctcgg ggatcaccgc gaacccggcc ctcggcgtcg
     1801 cgagcgacct cctggtcgac gcgggcggca ccgtaatcct cgccgagaca accgagctca
     1861 tcggagcgga gcacctcctg gccaggcgtg cggcgacccc cgcggtcgcc gcg
//
LOCUS       DASQGH010000118         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3266, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000118 DASQGH010000000
VERSION     DASQGH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3266"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1681
                     /locus_tag="VEW91_02295"
     CDS             74..1681
                     /locus_tag="VEW91_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYM90448.1"
                     /translation="MDSLLEHAADVESLAVLSRFRLEFHGCLTARGDELSGLADAVLC
                     ADHAVTSLVQVSLEPEFTRGHGALYDALSAGRIDDEGLFSLLAGELPQAVDGPEARAW
                     IAEHDVIDRGLLETALAGLPPGDAAQVRDACARWSRLRFAVDATAYPRPDAWCSPGRE
                     HVHNGACHCKGSSKTAPGWEYQFAAAIGHLRTAWAALTDVARTTPATRTAATIAQVKN
                     VLRRLRGGGEAAPLFVFDAGYSAAALAGGLAGCPVHVLVRLAAGSVFYAEPVAWEGKY
                     GRPARRGAAVHCLEPEDFEAAAAGSGPRGRKKPLPPNPEPDETLVLPGTPLYGTVRAG
                     AWHRVCPLIHGDRGWFAGRQELPALRGTLVHVTVERLPDGRDPHRAMWLWHSGPGPLS
                     LDELWRAYLARFDIEHAFKLVKGTLGLTAAKIRSPGQADRWARLLMAAHAQLLLARPL
                     AADLRRPWEKHPDPARPLTPGRVRRGFRNIRRDLGTPARVAKPSRPGPGRPKGSGKGP
                     APRYLLPGEADMPRIANTTLPREKVKT"
BASE COUNT          264 a          760 c          687 g          292 t
ORIGIN      
        1 gccgggcgtg agtgcggcct ccgcgtgatc atgtgttctc gccaaaggac acatgatcgc
       61 aacgaaggcc gtgatggaca gcttgctaga gcatgccgct gatgtcgagt ccctcgccgt
      121 gctgtcgcgg ttccggctgg agtttcatgg ctgcctgacc gcgcgcggtg atgagctgtc
      181 cgggctggcc gacgcggtgc tgtgcgcgga ccacgcggtg acgtcgctgg tgcaggtgtc
      241 cctggagccg gagttcaccc gcggccacgg cgccctctac gacgccctgt ccgccgggcg
      301 gatcgacgac gagggcctgt tctccctgct ggcgggggaa ctgccgcagg ccgtcgacgg
      361 gccggaggcc cgcgcgtgga tcgcggagca cgacgtcatc gaccgcggcc tgctggagac
      421 ggcgctggcc ggcctgcctc cgggcgacgc cgcgcaggtc cgggacgcat gcgcccggtg
      481 gagccggctg cggttcgccg tcgacgcgac cgcctatccc cggccggacg cgtggtgctc
      541 cccgggccgg gagcacgtcc acaacggcgc ctgtcactgc aagggctcgt ccaagaccgc
      601 cccgggctgg gagtaccagt tcgctgccgc gatcgggcac ctgcgcaccg cctgggccgc
      661 cctcaccgac gtcgcgcgca ccacgcccgc cacccgcaca gccgcgacga tcgcgcaggt
      721 gaagaacgtg ctgcgccgcc tgcgcggcgg cggggaggcc gcgccgctgt tcgtcttcga
      781 cgccggctac agcgccgccg ccctcgccgg cgggctcgcc ggctgccccg tccacgtcct
      841 ggtccggctc gccgccggct cggtgttcta cgccgagccc gtcgcctggg aaggcaagta
      901 cggccgcccc gcccgccgcg gcgctgcggt gcactgcctg gaaccggagg acttcgaggc
      961 cgccgcagcg ggcagcggcc cgcgcggccg gaagaaaccc ctgcccccga acccggagcc
     1021 ggatgagacc ctcgtcctgc cgggcacgcc gctttacggc actgtccgcg ccggggcctg
     1081 gcaccgcgtc tgcccgctga tccacgggga ccgcggctgg ttcgccggac ggcaggagct
     1141 gccggccctg cgcggcaccc tcgtccacgt caccgtcgag cggctgccgg acggccgcga
     1201 cccgcaccgg gcgatgtggc tgtggcactc cggccccggc cccctgtccc tggacgagct
     1261 ctggcgggcc tacctcgcga gattcgacat cgagcacgcc ttcaagctcg tcaagggaac
     1321 cctcggcctc accgccgcga agatccgcag tcccgggcag gcagaccgct gggcacggct
     1381 cctcatggcc gcgcacgccc agctcctgct cgcccggccg ctggccgccg acctccgccg
     1441 cccctgggag aagcaccccg accccgcccg gccgctgaca ccgggccggg tccgccgcgg
     1501 gtttcgcaac atccgccgtg acctcgggac tcccgcccgt gtcgcgaaac cctcccggcc
     1561 cggaccaggc aggcctaaag gcagcggcaa aggcccagct ccccgctacc tgcttcccgg
     1621 cgaagccgac atgcctcgca tcgcgaatac cacgctgccc agggaaaagg ttaaaactta
     1681 agcttagctt aggttttaac ctagattcgg gtcatatcag cgcatagtgg catggttcgc
     1741 ttatacgttt cggtttcttt cgcagaatgg ccgtttccct catgctgggt gttgcgacac
     1801 atcccgccat gaaaggaaac ggccgtgctt cataccctgc cacggcctgg ttccgggctg
     1861 caaacgcttc cgggaaccgg gccggccctg ctgccggcgt gcccggccgg gcaggccccg
     1921 gatccgggag tgttcgcgga cgcggtcgcc cgctaccggg cgctggagcg gctgcgggag
     1981 ttccggtcgc ggctgtacgg ctg
//
LOCUS       DASQGH010000119         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45812, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000119 DASQGH010000000
VERSION     DASQGH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45812"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VEW91_02300"
     CDS             complement(<1..481)
                     /locus_tag="VEW91_02300"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HYM90449.1"
                     /translation="MRTADEYRASLQDGRVVYYAGERVRDVTAHPALRGAVDHAAVDY
                     EMAADPRYRSLAVVPHPGGGEMSRYFAVPHTGEDLLARMELIETSTRLGGTIVPLLKE
                     IGTDALFALHLVAHEVDRARGTRYLDRVRAFYAHCRDRDLAMAVAQTDVKGDRSAGPS
                     "
     gene            complement(478..1173)
                     /locus_tag="VEW91_02305"
     CDS             complement(478..1173)
                     /locus_tag="VEW91_02305"
                     /inference="COORDINATES: protein motif:HMM:NF018952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HYM90450.1"
                     /translation="MGRTVGLVTIGQSPRPDLIEEYEQALPGARLIQAGALDDLSEPE
                     IQVLAPGGGDEVLVSRLRTGREVRLARRHLEPHLQSCLDRLSRDADLTILLCTGEFPT
                     LRSRGLVLVPQRVLHHVVAAAVDGLRGPDRGEAGLGVLIPDPAQQASAESRWGAWGPV
                     VTAAASPYRGMEAPEAAGGVLRAAGVGLVVMDCIGYTRPMRQRIARITGVPVILASAA
                     VAMIAREIVEGGA"
     gene            complement(1173..2258)
                     /locus_tag="VEW91_02310"
     CDS             complement(1173..2258)
                     /locus_tag="VEW91_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HYM90451.1"
                     /translation="MTPREATEAWEEQFLFPHAARSARSRGRARPEPPDRLRTVFQRD
                     RDRIIHSKAFRRLMHKTQVFLAPEGDHYRTRLTHTLEVAQIARTIARATRLNEDLTEA
                     IVLGHDLGHPPFGHAGEEALNQAMAAHGGFRHDAQSLRMVEILERRRARDGTYQEGLN
                     LTWEVRNGIGGHSKGTGDIEIFPDLDDAAPEKGLETVEGQLARVADRIAYVHHDTDDA
                     IRAGLIREDDVPEAVRRVLGQTRGQWVDTTVNDIVSCSTGTGRIQMSDEVRTALNGLK
                     DFLFEAVYQGSAAKAEVGKAQRLLIALFAYFVDHPGEIGEEARRLLDEGRATVPRAVC
                     DFLAGMTDRFAIRMGERLFLPRTWATL"
     gene            complement(2265..>2845)
                     /locus_tag="VEW91_02315"
     CDS             complement(2265..>2845)
                     /locus_tag="VEW91_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HYM90452.1"
                     /translation="LAKQGVRDHPEIMIPLVGHPNELRVVEERLRPLAARIMDEQGIR
                     VRYKFGTMIEIPRACVVADEIAELAEFFSFGSNDLTQLIFGFSRDDAEGKFLLRYVDE
                     KILPENPFQVLDARGVGALMEMAVRLGRRTRPDIELGICGEHGGDPQSIEFCHKLGLT
                     YVSCSPYRVPTARLAAAQAVLKEKQTRAELDR"
BASE COUNT          413 a         1044 c          952 g          436 t
ORIGIN      
        1 cgctcgggcc ggcgctccgg tcgcccttga cgtcggtctg cgccaccgcc atcgcgaggt
       61 cccggtcgcg gcagtgggcg tagaacgccc gcacgcggtc gaggtagcgc gtgccccgcg
      121 cgcggtccac ctcgtgcgcc accaggtgaa gggcgaacag cgcatccgtc ccgatctcct
      181 tcaggagcgg gacgatcgtc cccccgaggc gggtgctcgt ctcgatgagt tccatccgcg
      241 cgagcaggtc ctcaccggtg tggggaacgg caaagtaccg gctcatctcc ccgccgccgg
      301 gatgcgggac gaccgcgagc gaccgatacc ggggatccgc cgccatctcg tagtccacgg
      361 ccgcgtggtc gacggcgccc cgaagggcgg gatgcgcggt cacatcacga acgcgctccc
      421 ccgcatagta gacgacccgg ccgtcctgta ggctcgctcg gtactcgtcg gcggtcctca
      481 tgctcccccc tccacgatct cccgcgcgat catcgcgacg gcggcgctgg cgagaatcac
      541 cgggactccc gtgatgcgcg caatcctctg ccgcatcgga cgcgtgtacc cgatgcagtc
      601 catcacgacc aatcccacgc cggcggcccg gagcacccct cccgcggcct cgggcgcctc
      661 catcccccgg tagggggacg ccgccgcggt gaccaccggg ccccatgcgc cccaccggga
      721 ttccgcagac gcctgctggg ccgggtcggg gatgaggacg ccgagtccgg cctccccgcg
      781 atcgggaccg cgaaggccgt cgaccgcggc cgccacgaca tggtgcaaga cccgctgcgg
      841 cacgaggacc aagcctcggg aacgcagcgt gggaaactct ccggtgcaca ggaggatggt
      901 caggtcggcg tcccggctca agcgatccag gcaggattgg aggtggggtt cgagatggcg
      961 tcgggccagc cggacctcgc ggccggtccg cagccggctg accaggacct cgtccccgcc
     1021 gccgggcgcc aggacctgga tctcgggctc gctgaggtca tccagcgcgc cggcctggat
     1081 gagcctggcc cccgggaggg cctgctcgta ctcctcgatc aggtcggggc gcggcgactg
     1141 cccgatcgtc accaggccca cggtccgccc catcagagag tcgcccaggt gcgcggcagg
     1201 aagagccgct cgcccatccg gatggcgaag cggtcggtca tgcctgccag aaaatcgcac
     1261 accgcgcgcg ggaccgtcgc gcggccctca tcgaggagcc gccgggcctc ctccccgatc
     1321 tcgccgggat ggtcgacgaa atacgcaaac agggcgatca ggagccgctg ggccttgccc
     1381 acctcggcct tggcggcgga cccctggtag acggcctcga acaggaagtc cttgagcccg
     1441 ttgagcgcgg tacgcacctc gtcgctcatc tgaatccgcc cggtgccggt ggagcaggag
     1501 acgatgtcgt tgaccgtggt atcgacccac tgcccgcggg tctggcccag gaccctccgc
     1561 accgcctcgg gcacatcgtc ctcgcggatc agccccgccc ggatcgcgtc gtcagtatca
     1621 tggtggacat aggcgatccg gtcggcgacc cgggcgagct ggccttcgac cgtctcgagg
     1681 cctttctccg gggctgcgtc gtcgaggtcc gggaagatct cgatgtcccc cgtccccttg
     1741 gaatggcccc cgatgccgtt gcggacctcc cacgtgaggt tcaacccttc ctggtaggtc
     1801 ccgtcccggg cgcggcggcg ctccaggatc tccaccatcc gcaggctctg cgcatcgtgc
     1861 cggaaccctc cgtgcgccgc catcgcctgg ttcagggcct cttcgcccgc atgcccgaac
     1921 ggcgggtggc cgaggtcgtg gcccaggaca atcgcttcgg tcaggtcctc gttcaggcgg
     1981 gtcgcccgcg cgatcgtccg ggcgatctgc gccacctcca gcgtgtgggt caggcgcgtc
     2041 cggtagtggt cgccctcggg cgcgaggaac acctgcgtct tgtgcatcag gcgccgaaac
     2101 gccttcgaat ggatgatgcg gtcccggtcg cgctggaaca ccgtccggag acggtcgggc
     2161 ggttcggggc gggcccggcc gcgggaacgc gcgctgcggg cggcatgcgg gaacagaaac
     2221 tgctcttccc acgcctccgt cgcttcgcgc ggggtcatcg tgccctagcg gtcgagctcg
     2281 gcccgggtct gtttctcctt gagcacggcc tgcgcagcgg ccagccgggc cgtcggcacg
     2341 cggtacggag agcagcttac gtacgtgagc cccaacttat ggcagaactc gatgctctgg
     2401 ggatccccgc cgtgctcccc gcagatgccg agctcgatgt ccgggcgggt cctccgcccc
     2461 agccgaacgg ccatctccat caacgcgcca accccccgcg cgtcgagcac ctggaagggg
     2521 ttctcgggca ggatcttctc gtcgacgtac cggaggagaa acttcccttc ggcgtcgtcc
     2581 cggctgaacc caaagatcaa ctgggtcagg tcgttgctcc cgaacgagaa gaactctgcc
     2641 aactccgcga tctcgtccgc caccacgcag gcccgtggga tctcgatcat cgtgccgaac
     2701 ttgtatcgca cccggatccc ttgctcgtcc atgatccgcg cggcgagcgg ccgaagccgc
     2761 tcctccacca cccggagctc gttggggtgg ccaacgagcg ggatcatgat ctccgggtgg
     2821 tcccgcaccc cttgtttggc gagga
//
LOCUS       DASQGH010000120         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45840, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000120 DASQGH010000000
VERSION     DASQGH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45840"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /gene="secY"
                     /locus_tag="VEW91_02320"
     CDS             complement(<1..1183)
                     /gene="secY"
                     /locus_tag="VEW91_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HYM90453.1"
                     /translation="MIPGLANIIRIPDLRRKFLFTIGMLAVFRLGSHIPVPGVDVSRL
                     QSLFSQQGNVFGFIDLFVGGALSRFALFALGVFPYITASIIFSLLEVVFPSLKEMHRE
                     EGEAGRRRIGQYTLYLSVLLAVIQAVGQTILIRNVGALPDSRPLVVGEVVTTLVAGTM
                     VLTWMGSVMTEYGIGNGVSLIIFGGIVARLPNQLHQTIGLVRVGEQGMLFVIYDVAMI
                     LASIVLVIFVTQAVRKVPIQYAKRIVGRRMLGGQTTHLPIRIASAGVIPIIFAISVLQ
                     FPTTVAQFSAWPWLQRLGTALSISNPLGSGLYFVLIVVFTYFYTAVTFDPEDVSDNIK
                     KYGGFIPGIRPGRPTSDYLTRILERLTLVGALFLALIAVGPIMLGRFTGQLTLYLAGT
                     SL"
     gene            complement(1180..1647)
                     /gene="rplO"
                     /locus_tag="VEW91_02325"
     CDS             complement(1180..1647)
                     /gene="rplO"
                     /locus_tag="VEW91_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HYM90454.1"
                     /translation="MVRLDELKPARGSRRARRRIGRGGGSGRGNTAGRGTKGQKARTG
                     GQTRPGFEGGQLPLVKRVPYRRGVRGAGSVMTGGGPRPRMGVVNLGRLGVFPAGTEVT
                     PALLREAQLVREDRVKILASGELPHPLVVKAHAFSKAARARIEAAGGQVEVLA"
     gene            complement(1631..1840)
                     /gene="rpmD"
                     /locus_tag="VEW91_02330"
     CDS             complement(1631..1840)
                     /gene="rpmD"
                     /locus_tag="VEW91_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HYM90455.1"
                     /translation="MTAPAGRIRLTLRRSLIGRPEEQRRIVRALGLRRVGASRVHART
                     PGVLGAARQVAHLLSIEEVGHGQAR"
     gene            complement(1837..2472)
                     /gene="rpsE"
                     /locus_tag="VEW91_02335"
     CDS             complement(1837..2472)
                     /gene="rpsE"
                     /locus_tag="VEW91_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HYM90456.1"
                     /translation="MPRVKGKGAKGKGKIKKLRKLEAPPAPKPAGLEYLHRRVSAEAI
                     NLTTERAIWINRVAKVTKGAKRFNFSALVVVGDEHGHVGIGLGKAGEVPDAIRKGTED
                     ARKNLMEVSLAGTTIHHDIVSRFGAAKVLLRPASLGTGVIAGGPVRAVLEAAGVRDIL
                     TKSLGSSNPINQARATLKGLLDIRDPREVARIRNKTVEDLLGRRALGWQGA"
     gene            complement(2489..2857)
                     /gene="rplR"
                     /locus_tag="VEW91_02340"
     CDS             complement(2489..2857)
                     /gene="rplR"
                     /locus_tag="VEW91_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HYM90457.1"
                     /translation="MIKRGDRKESRRRRHLRIRQTVRGDAARPRLSVFRSLAHIYAQV
                     IDDARQQTVAAASSLDPEIRTEVAKAKKAEAGKLVGQLIARRAQAKGVRKVVFDRGGY
                     LYHGRVRAVADGAREGGLEF"
     gene            complement(2870..>3164)
                     /gene="rplF"
                     /locus_tag="VEW91_02345"
     CDS             complement(2870..>3164)
                     /gene="rplF"
                     /locus_tag="VEW91_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HYM90458.1"
                     /translation="QGVGYRAQKQGQALALQVGFSHPVEIVPPKGVALDAPQPNRIVV
                     SGVDKEQVGQTAASIRAIRKPDPYKGKGIRYLGERVRRKPGKAGKATVGVAKT"
BASE COUNT          462 a         1168 c         1050 g          484 t
ORIGIN      
        1 gcagcgaggt gccggccaga tagagggtca gctgccccgt gaaccgcccc agcatgatcg
       61 gcccgacggc gatcaaggcg aggaagaggg cgcccacgag cgtgagccgc tccaggatcc
      121 gggtgaggta gtcggaggtc ggccgcccgg gccggatccc cgggatgaac cccccgtact
      181 tcttgatgtt gtcggagacg tcctcggggt cgaacgtcac ggccgtatag aaatacgtga
      241 acacgacgat caagacgaaa tagagccccg agccgagggg gttgctgatg ctgagcgcgg
      301 tgccgaggcg ctgcagccac ggccacgcgg agaactgggc gaccgtcgtg gggaactgca
      361 gcaccgagat ggcgaagatg atggggatga cccccgccga cgcgatccgg atcggcaggt
      421 gggtcgtctg gcctcccagc atccgccgcc cgacgatgcg cttcgcgtac tggatcggga
      481 ccttgcgcac ggcctgggtc acgaagatga ccaggacgat gctggccagg atcatcgcca
      541 cgtcatagat cacaaacagc atcccttgct ctcccaccct caccagcccg atcgtctggt
      601 ggagctggtt ggggagccgc gcgacgatgc ccccgaagat aatgagggag acgccgttcc
      661 cgatcccgta ctcggtcatg accgagccca tccaggtgag caccatcgtc cccgccacca
      721 gggtcgtcac cacctcgccc acgacaagcg gcctggagtc cggcagcgcc ccgacgttcc
      781 ggatgaggat cgtctgcccg accgcctgga tcacggccag gagcaccgag aggtacaggg
      841 tgtactgccc gatcctccgc cggcccgcct cgccctcttc ccggtgcatc tccttcaagc
      901 tggggaagac cacctcgagg aggctgaaaa tgatcgacgc ggtgatgtag gggaacaccc
      961 cgagcgcgaa cagcgcaaac cgcgacagcg cgcccccgac gaacagatcg atgaacccga
     1021 acacgttgcc ctgctggctg aacaggctct gcaggcggct gacatccacc ccgggcaccg
     1081 gaatgtggga cccgaggcgg aacaccgcca gcatcccgat cgtgaacagg aacttccgcc
     1141 gcaggtccgg gatccggatg atgttcgcga ggcccggaat cacgcgagca cctccacctg
     1201 gccgccggcc gcctcgatcc gcgcccgggc ggctttgctg aacgcgtgcg ccttgacgac
     1261 cagcgggtgc ggcagctcgc cggaggcgag gatcttcacc cgatcctcgc gcaccaactg
     1321 cgcctcgcgg aggagggccg gggtgacctc ggtcccggcc gggaagacgc cgagccggcc
     1381 gaggttgacc acccccatcc gcggccgggg gccgccgccc gtcatcacgc tcccggcccc
     1441 gcggaccccc cggcggtacg ggacgcgctt cacgagcgga agctgcccgc cttcgaaccc
     1501 cggacgcgtc tgtccgccgg ttctcgcctt ctggcccttg gtgccgcgcc ccgcggtgtt
     1561 gcccctcccg gagccgcccc cccggccgat acggcgccgg gcgcggcggg agccgcgggc
     1621 gggtttcaat tcatcgagcc tgaccatgcc ccacctcttc gatagacagg agatgcgcga
     1681 cttgcctcgc cgcccccagc accccgggcg tccgggcgtg cacccggctc gcccccaccc
     1741 ggcggaggcc gagggcgcgc acgatccggc gctgctcctc gggccgcccg atgaggctgc
     1801 ggcgcagggt gaggcggatc cggccggccg gggccgtcat gcgccctgcc acccgagcgc
     1861 ccggcggccg agcaggtcct cgaccgtctt gttgcggatg cgggcgacct cccggggatc
     1921 ccggatatcg agcaacccct tgagcgtcgc gcgcgcctgg ttgatggggt tgctgctgcc
     1981 gagcgacttg gtgaggatgt cccggacccc cgcggcctcg agcaccgcgc gcaccggccc
     2041 cccggcgatc acgccggtgc cgagggaggc gggccggagg agcactttgg ccgccccgaa
     2101 tcgggagacg atatcatggt ggatcgtcgt gccggccagc gagacttcca tcaggttctt
     2161 ccgggcgtct tccgtgcctt tgcggatcgc gtccgggacc tcgccggctt tgccgagccc
     2221 gatccccacg tgtccgtgct cgtcgccgac caccacgagg gcgctgaagt tgaagcgctt
     2281 ggcgcccttc gtgaccttgg ccacgcggtt gatccagatc gcgcgttccg tggtcaggtt
     2341 gatcgcctcc gcgctcaccc gccggtggag atactccagc ccggccggct tcggcgccgg
     2401 gggcgcctcc agcttccgca gcttcttgat cttgcctttg cctttcgcgc ccttgccctt
     2461 caccctcggc atgcgatcct ctcccggatc agaactccag cccgccctcg cgggccccgt
     2521 cggccaccgc ccggacacgg ccgtgataca ggtagccgcc ccggtcgaac accaccttcc
     2581 ggaccccctt cgcctgagcc cgccgggcga tcagctggcc gacgagtttg cccgcctcgg
     2641 ccttcttggc cttggccacc tcggtgcgga tctcggggtc gagcgacgag gccgccgcca
     2701 ccgtctgctg gcgcgcatcg tcgatcacct gcgcgtagat gtgcgcgagg ctcctgaaca
     2761 ccgagagccg gggccgggcc gcgtctcccc ggacggtctg ccggatcctg aggtgccgcc
     2821 ggcggcgcga ttctttgcga tccccgcgtt tgatcatggc ccgccctctt taggtcttcg
     2881 ccacgccgac ggtggccttg ccggccttgc ccggcttgcg ccggacccgt tcgccgaggt
     2941 agcggatccc cttgcccttg tacggatccg gcttccggat cgcgcggatg ctcgccgcgg
     3001 tctggcccac ctgctctttg tcgacgccgc tcaccacgat gcggttgggc tgcggggcgt
     3061 cgagcgcgac acccttgggc gggacgatct ccaccgggtg cgaaaacccg acctggaggg
     3121 ccagggcctg cccctgcttc tgggcccggt agcccacccc ctgg
//
LOCUS       DASQGH010000121         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11858, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000121 DASQGH010000000
VERSION     DASQGH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11858"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1607)
                     /locus_tag="VEW91_02350"
     CDS             complement(603..1607)
                     /locus_tag="VEW91_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015892799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HYM90459.1"
                     /translation="MRYARVIQTIDCHAAGEPLRIITGGIPPIPGPTMLARRRYARER
                     LDHLRRMLMWEPRGHQDMYGCILTPPVSADAQAGVLFMHNEGYSTMCGHGIIGIVTVL
                     LETGHLDMVAPETHVTLDTPAGRVRARARIEGGRVRDVTMANVPSFAHAGCSVAIPGG
                     GTLPVTVAYGGAFYVLVDGPAAGRGPTPGPLPSLVAWSAEVKAAVAASLDAVHPEHPE
                     IRGLYGVVTSWPPQRSGADRTSLTVFADNQVDRSPCGTCTSALMAALYSTGRLGLNEP
                     FVNESLAGTRFTGRLVATATVGPYPAVVPEVTGSAAITGFHTFVLDPDDPLGEGFLLR
                     "
     gene            1704..>1833
                     /locus_tag="VEW91_02355"
     CDS             1704..>1833
                     /locus_tag="VEW91_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYM90460.1"
                     /translation="MTKRFLTPQEVAELLRVSSAAIHRLVRRGDLPAVRVGRASRVE"
BASE COUNT          329 a          592 c          595 g          317 t
ORIGIN      
        1 ctggccatgc gtagtccccc cccacgcgat tctctcgcca tcatcgatct cccgatgctt
       61 ttccccagcc gcaggtgaaa gcctgactat acaggaggtc tagacagatg cgcgacaata
      121 agtgtgggaa aaacaaagac agccgaaaaa attatcttct atctgtttct tttaaagttt
      181 agttcttcta tgttttacct ccaatcaaaa ttcatttatg aacttgacaa taaaggcgaa
      241 cacatggatg gcgaacaagt cgttcttcct acgggacggc acctggagtc aagggtatgg
      301 acgcttgtag cgtcgccgag cccgccggag ggggctccgg aatttcctcc attctcacga
      361 tcgccgatct gccatcgatc cgggccgacc ggtgcaggtg acagacagaa gcgatggtgt
      421 ccccgatctg cggccgcatc tctgtaagga atccgcacga gcactggtac catcccatat
      481 cggttcctcc ctcagccaga attgttgtgg tgtctccatt ctgggcctta gaaggaaccg
      541 acgctgtgaa tcggcgcacg atgggactgt gaaattcgtc acagggattg catacgccga
      601 tcctaccgca acaggaagcc ctcgcccagc ggatcatcgg ggtccaggac aaacgtgtgg
      661 aacccggtga tcgccgcgct cccggtgacc tcgggcacca cggcgggata gggcccgacg
      721 gtcgccgtcg ccaccaggcg ccccgtaaaa cgcgtgccgg cgaggctctc attgacgaag
      781 ggttcgttga gcccaagacg cccggtggaa tagagcgcgg ccatcagcgc ggaggtgcac
      841 gtgccgcagg gtgatcggtc gacctggttg tcggcaaaca cggtgaggct ggtccggtcg
      901 gctccggacc gctgcggggg ccaggaggtg acgacgccgt agaggccccg gatctcgggg
      961 tgctcgggat gcaccgcatc gagcgacgcc gcgaccgccg ccttcacttc tgcgctccac
     1021 gccacgaggg atgggagcgg gccgggggtg gggccgcgcc cggccgccgg gccatccacc
     1081 aagacgtaaa aggcaccgcc gtacgccacg gtcacgggga gggtgcctcc gccggggatg
     1141 gccacggaac accccgcgtg tgcaaacgac gggacattcg ccatcgtgac atcgcggacg
     1201 cgccccccct cgatccgggc gcgggcgcgc acccgtccgg cgggggtgtc cagcgtgacg
     1261 tgggtctccg gcgcgaccat gtcgaggtgc cctgtctcca gcagcacggt gacgatgccg
     1321 atgatgccgt gcccgcacat ggtgctgtag ccttcgttgt gcatgaagag gacgcccgcc
     1381 tgcgcgtccg cggagaccgg aggggtgaga atgcacccgt acatgtcctg gtgtccccgc
     1441 ggctcccaca tcagcatccg gcggaggtgg tccaggcgct cccgcgcgta ccggcgccgg
     1501 gccagcatcg tcgggccggg gatcggcggg attcccccgg tgatgatccg cagcggctct
     1561 ccggccgcgt ggcagtcgat ggtctggatg acgcgggcat agcgcatacg aaaccattcg
     1621 ctcctgccgc gtggtttccc gcactccacg gccggcgcgc tgcggcaggg agaacccgac
     1681 ccgaccgcca aacacttcgc agcatgacca agcggtttct caccccccag gaagtggccg
     1741 agttgctccg cgtttcttct gcggccatcc atcggctggt gcgccgggga gaccttcccg
     1801 cggtgcgggt cggccgggcc tcgcgggtgg agg
//
LOCUS       DASQGH010000122         6657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17523, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000122 DASQGH010000000
VERSION     DASQGH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17523"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VEW91_02360"
     CDS             complement(<1..718)
                     /locus_tag="VEW91_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM90461.1"
                     /translation="MPSAAPFVASSRPALRPRGLVVTMLVYALLVVIALVSVLPFLWA
                     VSSSLRPHQEIFASATPFSLRTFVPAARSLTLRAYAYIFTQDRFGRYMFNSAFVATAT
                     VALGLLVNSMAGFGFGRFQFAGRNLLFGLVLVTFLVPFEIIVLPLYLVIRGLHWQDTY
                     QALIFPAVADAFSIFLVRQFVREIPAELIDAARVDGASWWGIYRRITLPLVKPALITA
                     GLLQFMRQWDAFFWPLVAVSS"
     gene            complement(718..1569)
                     /locus_tag="VEW91_02365"
     CDS             complement(718..1569)
                     /locus_tag="VEW91_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM90462.1"
                     /translation="MVPYLFIAPAMLGLFVFKLYPIVAGLRMSFFDFQLISGSSRFIG
                     LENYRTMFADGTFWHALRNTLVFNAIVTPLQVAAALGLAVLVNRRLRGVNLFRSLYFI
                     PAILPLVVASVIWDLLYHPDNGVFNGILGTLGLPRQPFLVSAHQAMGAVIAMVTWKGA
                     GYWMVIFLAGLQSIPGQVYEAARLEGAAGGTLFWRITLPLLSGVLVFVTVADTAINFL
                     LFAPVYVMTQGGPSESTTVLMFEVYRNAFVYLHMGYATAIATVLLAMTLVVVAVQMRV
                     LPVEFEY"
     gene            complement(1663..2964)
                     /locus_tag="VEW91_02370"
     CDS             complement(1663..2964)
                     /locus_tag="VEW91_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYM90463.1"
                     /translation="MTREDRSRFSRRQALRLGGAAAVTAVLATRGALPARGAPQITVK
                     WISVERGEPRNSGMRKTVEVFGRNNPNVKVDLELVPFDQYFQKVAIALSSGSGIDVFD
                     VDSPLVTSYAVQDALLPLDEYIDKNDWLDFVGVERRTATVQGKIMSVPWSSSEQGLYY
                     NVEMLDAAGIKPARSVGDRWTWEQTLEAARKLTRTAPDGSTSVWGLTIEQVDRPYQIL
                     PLLESNGALPMAQDGKTVSGYINSPQAVEAVRFYGDLFNRWKVSPKKQLQDAFGNKQA
                     ALYLANTPWVNILRTRFPDLRFGVMPHPYFKKPVTPTGAWHLGVFKKTAHPKEAAELA
                     KTFGSREVAETAFKTMNYMPVRNSTFERFAADFQAAPWNLFLFEMKNTAVRRPATPAY
                     REYEDLLRTALRNVMEGGEPKAELDAAAGKIDREIAKYVRK"
     gene            complement(3012..4022)
                     /locus_tag="VEW91_02375"
     CDS             complement(3012..4022)
                     /locus_tag="VEW91_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HYM90464.1"
                     /translation="MTKRLIIDTDPGNGIPGSDIDDALAIVTALRSPEVELLGVTTVA
                     GNVEVVHATACALHLLEVAGRTDVPVCEGAATPLVADPAAIRAGIRARETSDAAARLW
                     THVRRPAPRRPVDRRRAAEFLIETVMAHPGEVTVVPIGPLTNIASAVRLEPRVASTVK
                     SIVWMGGAVRVPGTLTPVTELNASYDPEATHIVLSSGAPLTMVGLDVTTQTCLTLEDV
                     GRIGAVGTPLTDYLAEVVEPWVRYVMERRHLPGCWLHDPLAVCVAIDPSIVRTEPMWV
                     SVELASPRFRGQTVGWDMRFPYLFPVNEPNAHVCVAVDTARFMGLFLGTLGVRARTAS
                     GA"
     gene            4169..5101
                     /locus_tag="VEW91_02380"
     CDS             4169..5101
                     /locus_tag="VEW91_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYM90465.1"
                     /translation="MTELHVVLGASGGAGRAVVRELVARGKRVRAVTRAGALVPGAES
                     ARADAASPESVRDAVRGASVVYHCINVPYQQWARRLFPIADAIIEAAAAAGAKLVVMD
                     NLYMYGRVSGPMTERTPRQAQGKKGRLRAALERHLLEAHRSGKARLAIGRASDFLGPQ
                     ARSATMLLVVEPALAGRPASWLGTLDAPHTVSYLPDVGWGLATLGEREEAFGEVWHLP
                     AGPPLTGRQLIEMVFQELGRPARMKVISRPMMLAAGLFSSGIREAIEVLYQFEHPFVM
                     DATKFSRAFGSRVTPHRDAVRQTIEGSRGPGAPR"
     gene            complement(5103..6161)
                     /locus_tag="VEW91_02385"
     CDS             complement(5103..6161)
                     /locus_tag="VEW91_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90466.1"
                     /translation="MSPVGTATPVRLERVTKRFGAVTAVDDLSLELPAGRLVTLLGPS
                     GCGKTTILRIIAGLEHPTQGRVFIGEEDVTPLPASRRNVTMVFQSYALFPHLTVFENI
                     AYGLRITHIPRAEIVGRVADVLALVGLPRLEARFPAELSGGQQQRVALARALVMEPSV
                     LLFDEPLSNLDAKLRKRVRAEIRDLQQRIGVTSVYVTHDQGEALTISDVVVIINQGRV
                     EQIGTPVDLYQRPATQFVADFVGEANLLPGRYVAGRVTVGPLAFPFHQDGIADGAVLV
                     MAHPEAIAVRREGEGLPGRVRAAFFLGMTIEYLVDTPLGEVTVVEPRGTWGAITAGTD
                     VRLQFLESGVYLLPPGTT"
     gene            complement(6158..>6657)
                     /locus_tag="VEW91_02390"
     CDS             complement(6158..>6657)
                     /locus_tag="VEW91_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM90467.1"
                     /translation="SFAVPGTVMGVGYILAFNQGALLLTGTELIIVLAFVFRNMPVAI
                     RAAVAAVYQIDRSVEEASTMLRATSVPTLGRIVVPLVRSALLSGLVFAFVRAMTAISQ
                     VIFLITPAHNLATTLILSYVEYGNTGRGAALAAILTVFMVLVIVAFYALSRRLDPRAA
                     AEVSW"
BASE COUNT         1170 a         2282 c         2234 g          971 t
ORIGIN      
        1 gcgagctcac ggccacgagc ggccagaaga acgcatccca ctggcgcatg aactggagca
       61 ggcccgcggt gatgagcgcc ggcttgacga gcggcagggt gatgcgccgg tagatgcccc
      121 accaactcgc cccatccacg cgcgccgcat cgatgagctc ggcggggatc tcccggacga
      181 actgacggac caggaagatc gagaaggcat cggccaccgc cggaaagatc agggcctggt
      241 aggtatcctg ccagtgcagg ccgcgaatca ccaggtacag cggcagcacg atgatctcaa
      301 agggcaccag gaaggtcacc aggacgagtc caaagagcag gtttctcccg gcgaactgga
      361 accggccgaa cccaaacccg gccatggagt tgaccaggag gccgagcgcc acggtcgcgg
      421 tggcgacgaa cgcgctgttg aacatatacc ggccaaaccg atcctgggtg aagatgtacg
      481 cgtaggcgcg cagcgtgagc gaccgggcgg ccgggacgaa ggtccgcagg ctgaagggcg
      541 tggccgacgc gaagatctcc tggtggggac gcagcgagga gctcaccgcc cacaggaacg
      601 ggaggacgga caccagcgcg atcacgacca agagggcgta caccagcatg gtcacgacca
      661 ggccccgggg acgcagcgcc ggacgggagg aggcgacgaa aggagcggcg ctcggcatca
      721 atactcaaac tccacgggca gcacacgcat ctggaccgcc acgacgacga gcgtcatcgc
      781 caggaggacg gtcgcgatcg cggtggcgta ccccatatgc agatagacga aggcatttcg
      841 gtacacctcg aacatcagca ccgtcgtcga ctccgatgga ccgccctggg tcatgacgta
      901 gacgggggca aagaggagga aattgatcgc cgtatcggcc accgtgacga agacgagcac
      961 cccggagagc agcggcagcg tgatccgcca gaacagcgtc ccccccgcag ccccttccag
     1021 tctcgccgct tcgtacacct ggcccgggat gctctggagg cccgcgagga agatcaccat
     1081 ccagtagccg gcgcccttcc aggtcaccat ggcgatgacg gcgcccatcg cctggtgggc
     1141 actcaccaag aacggctggc gcgggagccc gagcgtgccg aggataccgt tgaacacgcc
     1201 gttgtccggg tgatacagga ggtcccagat cacgctggcg accaccagcg ggagaatggc
     1261 ggggatgaag tacagggacc ggaagagatt caccccccgg agcctccggt tgacgaggac
     1321 tgccaggccg agcgcggcgg ccacctggag gggcgtgacg atcgcattga agacgagcgt
     1381 gttccggagg gcgtgccaga aggtgccgtc ggcgaacatc gtccgatagt tctcgagacc
     1441 gataaaccgc gaactgccgg agatgagctg gaagtcgaag aagctcatgc gcagcccggc
     1501 gacgatcgga tagagcttga agacgaacag cccgagcatc gccggggcga tgaagaggta
     1561 cggcaccatc ccctgccgga ccctccccca acgacgatcg tgccgatcag gcgggaccgt
     1621 caccggcggg cgtcccgcgt gcgccgccgc ccccaccggg ggttatttcc tgacgtactt
     1681 ggcgatctct cggtcgatct tccccgccgc cgcatcgagc tcggctttcg gctccccgcc
     1741 ctccatcacg ttccggagcg ccgtcctcag gaggtcttca tactcgcggt aggctggggt
     1801 cgccggacgg cgcaccgcgg tgttcttcat ctcgaagagg aaaaggttcc agggagccgc
     1861 ctggaagtcg gccgcaaacc gctcgaacgt ggagttgcga accggcatgt agttcatcgt
     1921 cttgaacgcg gtctcggcca cctccctgct cccgaaggtc ttggccagct cggcggcttc
     1981 cttggggtgc gccgttttct tgaagacgcc cagatgccaa gcccccgtcg gcgtcaccgg
     2041 cttcttgaag taggggtgcg gcatcacgcc gaaccggaga tcggggaacc gcgttctcag
     2101 gatattgacc cagggggtgt tggcgaggta gagggcggcc tgcttgttcc cgaacgcgtc
     2161 ctggagctgc ttcttggggc tcaccttcca cctgttgaac aggtctccgt agaagcggac
     2221 cgcctccacg gcctgcgggg agttgatgta gccggacacc gtcttgccgt cctgcgccat
     2281 cgggagagcc ccgttcgact ccaggagggg gagaatctgg tagggccggt cgacctgctc
     2341 gatcgtcagt ccccagacgc tcgtggaccc atccggagcc gtccgggtca acttccgggc
     2401 ggcctccagg gtttgttccc aggtccagcg gtcccccacg ctccgagccg gcttgatccc
     2461 ggcggcatcg agcatctcga cgttgtagta aagcccctgc tcggaggagg accagggcac
     2521 cgacatgatc ttcccctgga ccgtggccgt ccgccgctcc accccgacaa agtccagcca
     2581 gtcgttctta tcgatgtact catcgagcgg gaggagcgca tcctggacgg cgtagctcgt
     2641 gacgagcggg ctgtctacat cgaacacgtc aatcccactc cccgagctca gggcaatggc
     2701 gaccttctga aagtactggt cgaacgggac cagttccagg tccaccttca cattcggatt
     2761 gttcctccca aagacttcca cggtcttgcg catcccgctg ttgcgcggct cgccccgctc
     2821 aaccgaaatc cacttcaccg tgatctgtgg ggccccccgc gcggggaggg ctccccgggt
     2881 cgcgagcacg gccgtcaccg ccgccgcccc gcccagccgg agcgcctggc gacgcgagaa
     2941 ccgacttcgg tcttccctcg tcatgaacac ccctcctcat ctgtcctgcc tgcgttccac
     3001 gcccgccgcc gtcacgcccc cgaggccgtc cgcgcccgga cgccaagcgt accgaggaac
     3061 agccccataa accgggcggt gtccaccgcc acgcagacgt gggcgttggg ctcattcacc
     3121 ggaaagaggt acgggaagcg catgtcccag ccgaccgtct ggccgcggaa ccgtgggctg
     3181 gccagttcga cactcaccca catcggctcg gtccggacga tgctcgggtc gatcgccacg
     3241 cacaccgcca gcggatcgtg gagccagcac cccgggaggt gacgccgttc catgacatag
     3301 cgcacccacg gctccacgac ctcggcgagg taatcggtga gcggggtccc caccgcgccg
     3361 atccgaccga cgtcctcgag cgtcaggcag gtctgcgtgg tcacatcgag gccgaccatc
     3421 gtcaggggcg ccccggacga gaggacgatg tgcgtcgcct cgggatcgta gctggcgttg
     3481 agctcggtca cgggcgtcag cgtcccggga acgcgcaccg ccccgcccat ccagacgatg
     3541 gacttcaccg ttgacgcaac ccggggctcg agccggaccg cgctcgcgat attcgtcagc
     3601 ggcccaatcg gcaccacggt cacctcgccg ggatgtgcca tcaccgtctc gatcaagaac
     3661 tcggcggcgc gccgccgatc gacagggcgg cggggcgcgg gacggcgcac gtgggtccag
     3721 agcctcgccg ccgcgtcact cgtctcccgc gcccggatgc cggccctgat cgccgccgga
     3781 tccgcgacga gcggggtggc cgccccttcg cagacgggga cgtcggtgcg acctgccacc
     3841 tcgagtaggt gaagcgcaca ggctgtggcg tgcacgacct cgacgttgcc ggcgacggtg
     3901 gtgacgccga ggagctccac ctcgggcgaa cggagggcag tgacaatggc aagggcatcg
     3961 tcgatgtcgg aacccgggat cccattgccc gggtcggtat cgataatgag acgtttcgtc
     4021 atggtcggag aaggtccgag catacgctgg aggccggcac agagccacag atcacattgg
     4081 cttcttcata cacccctctc cactcgtatt cctgcgtgat ctacctcgcg tgagggacgc
     4141 atgttccggt acaatagaag gaagaatcat gaccgagctg cacgtagtcc tcggagcgag
     4201 cggcggcgcg ggacgcgccg tcgtgcgcga gttggtcgca cgcggcaagc gggtacgcgc
     4261 ggtgacccgc gccggggccc tggtcccggg cgcggagtct gccagagccg acgcggcgag
     4321 cccggagagc gtgcgggacg cggtccgcgg ggcgtcggtc gtctaccact gcatcaatgt
     4381 cccctaccag cagtgggccc gccgcctgtt ccccatcgcg gatgcgatca tcgaggccgc
     4441 ggcggcggcg ggcgcaaagc tcgtggtcat ggacaatctt tacatgtacg gccgcgtttc
     4501 ggggcccatg accgagcgca ccccgcgcca ggcccagggg aagaaggggc ggctccgggc
     4561 cgcgctcgag cggcacctcc tggaggccca ccgctcgggc aaggcccgcc tcgcgatcgg
     4621 acgcgcctcc gattttctcg gcccccaggc gcgcagcgcg acgatgctcc tggtcgttga
     4681 gccggcgctc gcgggcaggc cggcgtcctg gctcggcact ctcgatgcgc cccatacggt
     4741 gagctacctg cccgatgtcg ggtgggggtt ggccacgctc ggggagcgcg aagaagcgtt
     4801 cggggaggtc tggcacctcc cggccggccc accgctcacc ggacgtcagt tgatcgagat
     4861 ggtgttccag gagctcggcc ggccggcccg gatgaaggtc atcagccggc cgatgatgct
     4921 cgccgccggc ctgttctcgt cggggatccg ggaagcgatc gaagtcctct accagttcga
     4981 gcatcccttt gtcatggatg ccacgaagtt ctcgcgcgcg tttgggagtc gcgtcacgcc
     5041 ccaccgggac gccgtccgcc agacgatcga aggcagccgg gggccgggcg cgccgcggta
     5101 ggtcaggtgg tcccgggcgg caggaggtag acccccgatt ccaggaactg caggcggacg
     5161 tcggtgccgg ccgtgatcgc cccccaggtg ccgcgcggct cgaccacggt gacctcgccg
     5221 aggggggtgt cgacgaggta ctcgatcgtc atccccaaga aaaacgccgc ccggacgcgc
     5281 cccggcagcc cctccccctc ccgccgcacc gcgatcgcct cgggatgggc catgacgagg
     5341 acggccccat cggcgatccc gtcttggtgg aacggaaacg ccagcgggcc cacggtgacg
     5401 cggcccgcta cgtaccggcc cggcagtagg ttggcctcgc cgacgaagtc cgcgacgaac
     5461 tgcgtcgccg gccgttggta gaggtcgacc ggcgtcccga tctgctccac ccgcccctgg
     5521 ttgatgatga cgaccacatc cgaaatggtc agcgcttccc cctggtcgtg cgtgacgtag
     5581 acgctcgtga ccccgatccg ctgttggagg tcgcggatct cggcccgcac acgcttccgc
     5641 agcttcgcgt cgaggttgga gaggggctca tcgaacagga gaacgctggg ctccatgacc
     5701 agcgcccggg cgagggcgac gcgctgctgc tggcccccgg acagctccgc cgggaaccgc
     5761 gcttccagcc gggggaggcc caccagggcg agcacgtcgg ccacgcggcc gacgatctcc
     5821 gcgcgtggga tgtgcgtgat gcggagcccg tacgcgatgt tctcgaagac ggtgagatgg
     5881 gggaacagcg cgtacgactg gaacaccatc gtcacgttgc gccggctcgc cggcagcggc
     5941 gtcacgtcct cctcgccgat gaacacccgc ccctgggtcg gatgctcgag cccggcgatg
     6001 atccggagga ttgtcgtctt cccgcacccc gagggcccca gcaaggtcac cagcctgcca
     6061 gcgggcagct cgaggctgag atcgtcgacg gcggtcacgg ctccgaaccg cttggtcacg
     6121 cgctccagcc gcaccggggt ggccgtcccc acagggctca ccagctcacc tccgccgccg
     6181 cccgcgggtc gaggcggcgg ctcagggcgt agaacgccac gatcaccagg accatgaaca
     6241 ccgtcagtat cgccgccagc gccgcgcccc gccccgtgtt gccatattcc acgtacgaga
     6301 ggatcagggt cgtcgccagg ttgtgcgcgg gcgtgatcag gaagatcacc tggctgatcg
     6361 cggtcatcgc ccgcacgaac gcaaacacga gcccggacag gagcgcggac cggaccaggg
     6421 gcacgacgat gcgccccagg gtcggcaccg aggtcgcgcg cagcatcgtg gacgcttcct
     6481 ccaccgatcg gtcgatctgg tagaccgcgg ccaccgcggc ccggatcgcg accggcatgt
     6541 tgcggaacac aaacgcgagc acgatgatca gttcggtgcc ggtcaggagg agcgcgccct
     6601 ggttgaaggc caggatgtac ccgaccccca tcacggtgcc cgggaccgcg aacgaca
//
LOCUS       DASQGH010000123         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_487, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000123 DASQGH010000000
VERSION     DASQGH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VEW91_02395"
     CDS             complement(<1..507)
                     /locus_tag="VEW91_02395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYM90468.1"
                     /translation="MRRLVTSRRGYQGRWISVRLDEVALPGGRRAVYEVVEHPGAVAI
                     VALTADRHLLLVRQFRQPVGTELLEVPAGTLEPGETPLACARRELAEEVGRAAARWER
                     LISFYPSPGVLSEELHVFLAEDLRDEPAEREEDEDLEVLSLPLEEAFRQIGAGEIRDA
                     KSIIGITVA"
     gene            complement(529..1431)
                     /gene="ilvE"
                     /locus_tag="VEW91_02400"
     CDS             complement(529..1431)
                     /gene="ilvE"
                     /locus_tag="VEW91_02400"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HYM90469.1"
                     /translation="MGSIVYVNGRFVSKEDATVSVFDHGFLYGDGVFEGIRVYHGRIF
                     KLDAHVNRLFESAYTLGIQIPLSRQEMTQAIVETVRRTGLRDAYIRPVVSRGTGDLGI
                     DPRKCPRPNIIVIVDSIQLYAEEAYRKGLRMITTSTRQRPVDVLNPRIKTCNYLNNIL
                     ARMEVNLVGVDEGLMLTTQGYVAECTADNIFIVKAGRVLTPPPHLGILQGVTRQTVLD
                     LCGPLGVPAAEQILTLHDMYGADEAFLTGTGAELGPVVELDGRKIGTGRPGPVTLRIL
                     AAFRELAAREGTPVYEASGAPAST"
     gene            complement(1469..>1903)
                     /locus_tag="VEW91_02405"
     CDS             complement(1469..>1903)
                     /locus_tag="VEW91_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYM90470.1"
                     /translation="DPATPYPVIDLLPEQKAVTEKGGTMRLGLYPCRLTPGSRAGEAY
                     GVAEVQERHRHRFEVNNDYLPVLTQHGLRVTGLYPHRQLVEIVELADHPWFVGTQFHA
                     EYRSRPNRPHPLYREFVAAALRHAQHRAARLPKEPSAPAANR"
BASE COUNT          281 a          664 c          659 g          299 t
ORIGIN      
        1 cgcgactgtg atgccgatga tcgacttggc atcccgaatc tctccggcgc cgatctggcg
       61 gaaggcctcc tcgagcggca gggagagcac ctcgaggtcc tcgtcctcct cgcgctccgc
      121 cggctcatcc cgcagatcct cggccaggaa cacgtgcaac tcctccgaga gcacgccggg
      181 ggagggatag aacgagatga gccgttccca gcgggccgcc gcccgcccga cttcctcggc
      241 cagttcgcgc cgggcgcagg ccagcggggt ctcgccgggc tccagggtcc ccgccgggac
      301 ctcgagcagt tcggtcccga cgggctggcg gaactgccgg acgaggagaa ggtgccggtc
      361 tgcggtcagc gcgacgatgg cgaccgcgcc cggatgctcg acaacctcgt agacggcccg
      421 gcgaccgccg ggcagggcca cctcgtcgag gcgcaccgag atccaccgcc cctggtagcc
      481 ccgccggctg gtgaccaggc gccgcatcag gcgcgggtca cgcgcagctc acgtagaggc
      541 gggagccccg gaggcttcgt agacgggtgt gccttctcgc gcggccaact cccggaacgc
      601 cgccaggatg cgcagggtca ccgggccggg ccgcccggtc ccgatcttcc ggccgtccag
      661 ttccaccacc gggccgagtt ccgccccggt gccggtcagg aaggcctcgt cggccccgta
      721 catatcgtgc agggtcagga tctgctccgc ggccgggacc ccgagcggcc cgcataggtc
      781 gagcaccgtc tggcgggtaa ccccctggag gatgcccagg tgcgggggcg gggtcagcac
      841 ccggccggcc ttgacgatga agatattgtc ggccgtgcac tcggccacat acccctgggt
      901 ggtgagcatc aacccctcgt cgaccccgac caggttgacc tccatccgcg cgagaatgtt
      961 gttcaggtag ttgcacgtct tgatgcgcgg gttgagcaca tccaccggcc gctggcgcgt
     1021 ggacgtggtg atcatccgca gccccttgcg gtacgcctcc tcggcataca gctggatcga
     1081 gtccacgatg acgatgatgt tcggccgggg gcatttgcgg ggatcgatcc cgagatcgcc
     1141 cgtgccccgc gagacgaccg ggcggatgta cgcgtcccgg agcccggtgc gccgcaccgt
     1201 ctcgacgatc gcctgcgtca tctcctgtcg gctgagggga atctggatcc ccaacgtgta
     1261 ggcggactcg aagagccggt tcacgtgcgc atcgagcttg aagatccgcc cgtggtagac
     1321 ccggatccct tcgaacacgc cgtccccgta gaggaatccg tgatcaaaga ccgagacggt
     1381 cgcgtcctct ttcgagacga accgaccgtt gacgtagacg atgctgccca ttgccttctc
     1441 ctctccgcgc gcgggtccgg actctcgctc accggttcgc cgctggcgca gacggctcct
     1501 ttggcaggcg ggccgcgcgg tgctgggcgt gccgcagcgc ggccgcaacg aactcgcgat
     1561 agagtgggtg gggccgattc ggccgcgatc ggtactccgc gtggaactgc gtgccgacga
     1621 accaggggtg gtcggcgagc tcgacgatct cgacgagctg gcggtggggg tacagcccgg
     1681 tgacccggag cccgtgctgt gtcaggacgg gcaggtagtc gttgttcacc tcgaaccggt
     1741 ggcggtgccg ttcctggacc tccgccaccc cgtacgcctc ccccgcgcgc gacccggggg
     1801 tgaggcggca cgggtagagc cccagccgca tcgtcccgcc tttctcggtg acggctttct
     1861 gctccggcag cagatcgatg accgggtagg gcgtcgcggg atc
//
LOCUS       DASQGH010000124         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9038, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000124 DASQGH010000000
VERSION     DASQGH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9038"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1403)
                     /locus_tag="VEW91_02410"
     CDS             complement(93..1403)
                     /locus_tag="VEW91_02410"
                     /inference="COORDINATES: protein motif:HMM:NF033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HYM90471.1"
                     /translation="MPETSPRLPFEIDDAIDPTLVTGRAGVPLVIELFRQLGVAQVID
                     THVPVKQRQRGLPPSQLVESLIALWTSGGDRCQDLTTLREDQALAALLGYPLPAATTV
                     RDFLEAFHVENGPLWQAGPEAVIPEESAPLAGLGQATRTLVAGVQRGAPTDTATLDVD
                     ATLVESHKDAATVAYDGTRGYQPVVVLWAEQDLILHDQFRDGHVPAGCGNVRVLEQAV
                     ANLPQGITQISLRADSALYETAVLRWCETKQIAYAISADCSDQLKAEIVRLPETAWQV
                     EREEPEAIRAWAEVPYVPDDGDHRQARPCVRRYLAVRVQKRQGSLFADGSSLHYFAVV
                     TNRAGDGLALLRWHRAKAGTVEHTHHVLKTELAAAALPSGKFGANAAWFRLNTLTYNL
                     LSALKRLTLPGDLRTARPKRLRFLLFNTVGKVVAHARRTLLRLS"
BASE COUNT          262 a          578 c          595 g          267 t
ORIGIN      
        1 aagggacgag accttgcctt aatctccggc taagccgggg cgagccgggc gatcttgccc
       61 cgcacccgga cgagtagagc gtgttggaaa gatcacgaga gccgcaagag cgtccggcgg
      121 gcgtgcgcca cgaccttccc gaccgtgttg aagagcaaga agcgcagccg cttcggccgg
      181 gcggtccgga gatctccggg taaggtgagc cgcttgagcg ccgagagcag gttgtacgtc
      241 agcgtgttga gccggaacca ggcggcgttc gccccgaact tcccgctggg gagggcggcg
      301 gccgccaact cggtcttgag gacgtgatgc gtgtgctcca ccgtgccggc cttcgcgcgg
      361 tgccaccgga ggagggccaa cccgtccccc gcccggttcg tgaccacggc gaagtagtgg
      421 aggctgctcc cgtcggcgaa gaggctgcct tgacgcttct ggacccgcac ggcgaggtag
      481 cggcggacgc aggggcgggc ctgccggtga tccccgtcgt cggggacgta ggggacctcg
      541 gcccaggcgc ggatcgcctc cggctcctcc cgctccacct gccaggcggt ctcggggagg
      601 cgaacgatct cggccttgag ctggtcggag caatcggcgc tgatggcgta ggcaatctgc
      661 ttggtctcgc accagcggag gacggcggtc tcgtagagcg cactgtcggc gcgcagggag
      721 atctgggtga ttccctgggg caggttggcc acggcctgtt cgagcacgcg cacgttgccg
      781 cagccggccg gcacatggcc atcgcggaac tgatcatgga ggatcaggtc ctgctcggcc
      841 cagagcacga ccacgggctg gtagccccgg gtgccgtcat aggccacggt ggcggcgtcc
      901 ttgtggctct ccacgagcgt tgcgtccaca tcgagggtcg cggtgtccgt cggcgccccc
      961 cgctggacgc ccgcgaccag cgtgcgggtc gcctgaccga ggcccgccag gggcgcagac
     1021 tcctccggga tcaccgcctc gggcccggcc tgccacaggg ggccgttctc gacgtggaag
     1081 gcctccagga agtcccggac cgtggtcgcg gcgggcaggg ggtacccgag cagcgcggcc
     1141 aaggcctgat cctcgcggag cgtcgtgaga tcctggcagc ggtccccgcc gctcgtccac
     1201 aacgcgatca ggctctcgac gagctgcgag ggcggcaagc cccgctgccg ctgcttgacc
     1261 ggaacgtggg tatcgatcac ctgggcgaca cccaactgcc ggaacaactc gatcacgagc
     1321 gggacccccg cgcggccggt aaccaacgtt gggtcaatgg cgtcatcgat ctcgaacggc
     1381 aatcgcggcg acgtctcggg catcggtctc ctccgtggcg ggtctgcacc cactgggtga
     1441 aactaagcaa acccgatgcc agcggtgcgg accgcttacg tcgcgggctg ccgcgcattc
     1501 cgttcgaccg ctcaacttct tagccggaga atagggctag tcctgatccc ccggcctcgc
     1561 gcggctcgcc catccagctt tcccttcgtc ccaaatcttt tcagcggcca gtcactggtc
     1621 ttccctttcc gagggcgtgt cccaagagaa actcgcggat ggccccggcg acctcgcccg
     1681 gacgctccag gatcaggtga tg
//
LOCUS       DASQGH010000125         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11895, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000125 DASQGH010000000
VERSION     DASQGH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11895"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VEW91_02415"
     CDS             <1..349
                     /locus_tag="VEW91_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM90472.1"
                     /translation="KDISPTNLANGIRAVHNGTTMINPAIARRLVEDLFAGNGTAATS
                     SRHQYGLTQREIDVLAGVAKGLSDKEIASMLFLSESTVKTHLRTIYHKLKLRNRAQAA
                     AFMVDKGLLQGSP"
     gene            complement(364..1242)
                     /locus_tag="VEW91_02420"
     CDS             complement(364..1242)
                     /locus_tag="VEW91_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90473.1"
                     /translation="MGEASLPLPRPAAPGPRGSTLGRRLRASWQVRIGGSILVALISV
                     AFLAPYLAPYDPSQPNIPERFGGPSRAHLFGTDNLGRDLFSRVVYGSRVSLQIGLISV
                     GIAFAWGVALGLLAGFYPRLEPPIMRSMDVLLAFPGLLLAIAILGALGPSLTNAMIAV
                     GIAGVPSFVRITRSAVLRVRTQEYVEASRAVGAADVRILVRHVLPNITSEIIVTTTLG
                     LAGAILAASVLSFLGLGAQPPTPEWGAMVFSAKDFLDLAPHSTLFPIVAIFVTVLGFN
                     FLGDGLRDALDPRLYR"
     gene            complement(1235..2173)
                     /locus_tag="VEW91_02425"
     CDS             complement(1235..2173)
                     /locus_tag="VEW91_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90474.1"
                     /translation="MTGYLAGRLLLAVPVLLGVSVLVFLILHLTPGNPALVVAGPDAP
                     PEVVREVERALGLDQPLYVQYARYLGRILRGDFGRSIRSREPVLERLLVTFPVTLALA
                     GVGVAFTTLVSVPMGILAAYHRNSPIDLATIFVVLAGSAMPVFAIGLILLWLFAVTLR
                     WFPISGFAPLTTFEGWRHMALPAVTVSSGTIALLARLTRSSMLEVLNQDYVRTAKAKG
                     VREAAVVVRHAFRNALLPVVTVIGLQFGLLLGGAVVTESIFSLPGMGRLLVQAILGRD
                     FPIVQGAVLLAALTFVLTNLLVDLVYAVVDPRIRYG"
     gene            complement(2208..>2677)
                     /locus_tag="VEW91_02430"
     CDS             complement(2208..>2677)
                     /locus_tag="VEW91_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90475.1"
                     /translation="LSLLFLYSPGPENEALVNLVQAELAGVGVNARPQRLEWTALLAA
                     LRAGQHDIFLINVRYVTADILYFYFHSKQRPAPNRIDWADPETDRLLELSRSSTNDGE
                     RLGAYQKLQQIVVENAIWVPLVHQQRVVLASPRLEIPRMHANVLYKMLDLTLK"
BASE COUNT          481 a          902 c          972 g          322 t
ORIGIN      
        1 gaaggatatc tctccgacga acctcgcgaa cggcatccgg gccgtccata acggcaccac
       61 gatgatcaat ccggcgattg ccaggcggct tgtcgaggac ctcttcgccg ggaacggcac
      121 cgccgcgacc tcgtcgcgac accagtacgg gctgacccag cgcgagatcg atgtcctcgc
      181 gggggtggcg aagggcctga gcgacaagga gatcgcctcg atgctcttcc tgtccgagtc
      241 gacggtgaag acgcacctgc ggacgatcta tcacaagctc aaactgcgca accgcgccca
      301 ggccgccgcc ttcatggtgg acaaaggact cctgcagggc tccccatagt ccgggccccg
      361 cgctcagcgg tagaggcgtg ggtcgagcgc gtcccgcagc ccgtccccga ggaagttgaa
      421 cccgaggacc gtcacaaaga tcgccacgat cgggaacagc gtgctgtggg gggcgaggtc
      481 caggaagtcc ttcgcggaaa agaccatcgc gccccactcc ggcgtcgggg gttgcgcgcc
      541 cagcccgagg aacgagagca ccgaggccgc caggatcgcc cccgccaggc cgagcgtcgt
      601 ggtcacaatg atctcggagg tgatgttggg gagcacgtgc cgcacgagaa tccggacgtc
      661 ggcggccccg accgcccggc tggcctcgac atactcctgg gtccgcacgc ggagcacggc
      721 actccgggtg atccgcacga acgagggcac cccggcgatc cccaccgcaa tcatcgcgtt
      781 ggtgaggctc ggcccgaggg cccccaggat cgcgatcgcg agcagcagcc ccggaaacgc
      841 cagcaggacg tccatgctcc gcatgatcgg gggctccagc cgcggataga atcccgcgag
      901 gagcccgagg gcgacccccc aggcgaaggc gatgcccacc gagatcaggc cgatctggag
      961 ggagacgcgg ctcccgtaga cgacccggct gaagaggtcc cggccgaggt tgtcggtgcc
     1021 gaagagatgc gcgcgggacg gcccgccaaa gcgctccggg atgttcggct gggaggggtc
     1081 gtacggggcg aggtacgggg ccaggaacgc caccgagatg agcgccacca ggatcgatcc
     1141 gccgatgcgg acctgccacg aggcccggag gcgccgcccc agcgtcgagc cccggggacc
     1201 cggcgccgcc ggcctcggga gcggcaggga tgcctcaccc atatcggatg cgcggatcca
     1261 cgaccgcgta gacgaggtcg acgagcaggt tggtcaggac gaacgtgagc gccgcgagga
     1321 ggaccgcgcc ctggacgatg gggaagtccc gcccgaggat cgcttgcacg aggagccggc
     1381 ccatgccggg gagcgagaag atgctctcgg tcacgacggc cccgccgagc aggagcccga
     1441 actgcagccc gatcaccgtc acgaccggca agagggcgtt ccgaaaggcg tgccggacga
     1501 cgaccgcggc ctcccgcacc cccttggcct tggccgtgcg cacgtagtcc tggttcagca
     1561 cctccagcat cgacgaccgg gtcagccggg cgaggagcgc gatcgtgccg ctgctgaccg
     1621 tgacggcggg gagggccatg tggcgccagc cctcgaaggt cgtgagcggg gcgaacccgg
     1681 agatcgggaa ccaccggagg gtgacggcaa acagccacag gaggatcagg ccgatcgcga
     1741 agaccggcat cgccgatccg gcgagcacga cgaagatcgt cgcgaggtcg atcggggagt
     1801 tgcggtggta cgcggccagg atccccatcg gcacgctcac gagcgtggtg aacgcgacgc
     1861 cgaccccggc gagcgcgagc gtgacgggga acgtcacgag gaggcgctcg agcaccggct
     1921 cccgcgaccg gatcgagcgc ccgaagtccc cccgcaggat gcggccgaga tagcgcgcgt
     1981 actggacgta cagcggctgg tcgagcccga gggcgcgctc gacctcgcgc accacctccg
     2041 gcggggcgtc cgggccggcc accacgagcg ccgggttgcc cggggtcaga tggaggatca
     2101 ggaacaccag gaccgagacc cccagcagca ccgggaccgc cagcaggagg cgacccgcga
     2161 ggtatccggt catcgccgcg ggggctgggc gacgggggag gggccggcta cttgagcgtc
     2221 aggtccagca tcttgtagag cacgttcgcg tgcatccgcg ggatctccag cctcggcgag
     2281 gcgagcacca cgcgctgctg atgcacgagc ggcacccaga tggcattctc gacgacgatc
     2341 tgctggagct tctggtaggc gcccaggcgc tccccgtcgt tggtgctgga gcggctcagc
     2401 tccaggagcc ggtcggtctc cggatcggcc cagtcgatcc ggttgggcgc cggccgctgc
     2461 ttgctgtgga agtagaagta caggatgtcc gcggtcacgt accggacgtt gatcaggaag
     2521 atgtcgtgct ggcccgcgcg gagcgccgcg agcagcgcgg tccactcgag ccgctggggc
     2581 cgggcgttca cgccgacgcc ggcgagctcg gcctgcacga ggttgaccag cgcctcgttc
     2641 tccggcccgg gcgagtacag aaagaggagc gacagcg
//
LOCUS       DASQGH010000126         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_505, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000126 DASQGH010000000
VERSION     DASQGH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VEW91_02435"
     CDS             <1..905
                     /locus_tag="VEW91_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90476.1"
                     /translation="GTLVLVYIMLGLGLNVVVGYAGLLDLGYAAFFAIGAYTTALLMV
                     SYHIAFWLTLPVATFFTALSGVIIGTPTLRMRSDYLAIVTLGFGEIVRIAATNLTFTG
                     GPEGVYGVPHPTIGGFVLRTPQATYWLGMAFALLVLVGSYNLARSRLGRAWLAIREDE
                     AAAQAAGIYPVYYKLMAYVLGAIFAGAAGSFFAVKLSAINPSSFTFIQSVTILMVIIL
                     GGMGSLPGVMLGAAVIVVLPEALRAFDQVRMFAFGIGLIVMMLLRPQGLWPSRIGQIV
                     RRTVALSEASAAAGVAVDTASTTE"
     gene            918..1721
                     /locus_tag="VEW91_02440"
     CDS             918..1721
                     /locus_tag="VEW91_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90477.1"
                     /translation="MTAASAAPTVETGAILGVERLTMRFGGLVAVSDVSFTVRPREVL
                     SIIGPNGAGKTTVFNCITGFLRPTTGRIAFEGTAITGLRPDLVVKRGIARTFQMIRLF
                     PDLDVDENVMIGLHGRTRASTLDSLLHTPLHRREEAWAHEETYRWLAFVGLEARAHQR
                     ARGLAYGNQRRLEIARALATAPRLLILDEPASGMNPAEKAELVQLVSRIRDSGITVLF
                     IEHDMSVVMGISDRIIVLDYGVQIAEGVPEEIQRNPKVIEAYLGREDEG"
     gene            1718..>2445
                     /locus_tag="VEW91_02445"
     CDS             1718..>2445
                     /locus_tag="VEW91_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90478.1"
                     /translation="MSLLEVRNLVTAYGSVEALHGISFRVDEGEIVALLGANGAGKTT
                     ALRTISGLLRPRSGEVRFADERIDTRPAHEIVRLGLTHVPEGRWIFTLMTVDENLRLG
                     AYAETHLPREALERVFTLFPRLAERRGQLAGTLSGGEQQMLAMARALMARPRLLLLDE
                     PSMGLAPVLVRAIFQTVAAINREGTTVLLVEQNANAALRLARRAYVLENGRIALEGLG
                     SELAKNEAVRRAYLGIGTRLSAPEG"
BASE COUNT          350 a          836 c          857 g          402 t
ORIGIN      
        1 tcggcacgct cgtcctcgtc tacatcatgc tgggcttggg actcaatgtc gtggtgggct
       61 acgccgggct gctcgacctc gggtacgcgg cctttttcgc gatcggcgcc tacacgacgg
      121 cgctcctgat ggtcagctac cacattgcgt tctggctcac gctgccggtg gccacgttct
      181 tcaccgcgct ctccggcgtc atcatcggca cgccgaccct gcggatgcgc agcgactacc
      241 tcgcgatcgt cacgctggga tttggcgaga tcgtccgcat cgcggccacg aatctgacgt
      301 ttaccggcgg accggagggc gtctacggag tcccccaccc gacgatcggc gggttcgtct
      361 tgcgcactcc gcaggcgacg tactggctcg ggatggcgtt tgcgctcctc gtcctcgtgg
      421 gcagctataa cctcgcccgg tcgcggctgg ggcgggcctg gctcgcgatc cgggaggacg
      481 aggcggcggc ccaggcggcc ggcatctacc ccgtgtacta caagctgatg gcctacgtgc
      541 tcggggccat cttcgccggc gcggcgggat cgttcttcgc ggtgaagctc agcgcgatca
      601 acccgtcgag cttcacgttc atccaatcgg tgaccatcct gatggtcatc atcctcggcg
      661 ggatggggag cctgcccggg gtgatgctgg gcgccgccgt catcgtcgtt ctgcccgagg
      721 cgctccgggc gttcgaccag gtccggatgt tcgcgttcgg gatcggcctc atcgtcatga
      781 tgctcttgcg cccgcagggc ctgtggccga gccggatcgg gcagatcgtc cggcggaccg
      841 ttgctctgtc ggaggcgagt gccgcggcag gggtcgcggt cgacaccgcc tccacgacgg
      901 aatagcgcga ggaccccatg accgccgcat ccgctgctcc gaccgtagag accggcgcca
      961 tcctcggggt cgagcgcctc acgatgcgct tcggcggcct ggtcgccgtc tccgacgtgt
     1021 cgtttaccgt ccggccgcgc gaggtcctca gcatcatcgg ccccaacggg gccgggaaga
     1081 cgacggtatt caactgcatc acgggctttc tccgccccac gaccggccgc atcgccttcg
     1141 aggggacggc catcacgggg ctccgtcccg atctcgtcgt gaagcggggg atcgcccgca
     1201 ccttccagat gatccgcctg ttccccgacc tcgacgtgga tgagaacgtg atgatcggcc
     1261 tgcacggccg gacccgcgcg agcacgctcg actcgctcct gcacacgccc ctgcaccggc
     1321 gggaggaggc gtgggcccac gaggagacct accggtggct ggcgttcgtc ggcctggagg
     1381 cgcgcgcgca ccagcgggcc cgggggctgg cgtacggaaa ccagcggcgg ctggagatcg
     1441 cccgggcgct ggcgaccgcc ccccggctcc tcatcctcga cgagccggcg agcggcatga
     1501 acccggcgga gaaggctgag ctcgtgcagc tcgtcagccg gatccgcgac tccggcatca
     1561 cggtcctctt catcgagcac gacatgagcg tcgtcatggg aatcagcgac cgcatcatcg
     1621 tcctggatta cggggtgcag atcgccgagg gcgtcccgga ggagatccag cggaacccga
     1681 aggtgatcga ggcctacctc ggccgggagg acgagggatg agcctgctcg aagtgcggaa
     1741 cctcgtcacc gcgtatggga gcgttgaggc cctccacggc atctcattcc gcgtagacga
     1801 gggtgagatc gtcgcgctcc tcggggcgaa cggggccggg aagacgacgg cgcttcgaac
     1861 gatctccggg ctcctgcgcc cgcggtccgg ggaggtgcgc ttcgcggacg agcggatcga
     1921 cacccgtccg gcccacgaga tcgtccgcct ggggctgacg cacgtccccg aagggcggtg
     1981 gatcttcacc ctcatgacgg ttgacgagaa tctgcgcctc ggggcctacg cggagacgca
     2041 cctgccccgg gaggccctgg agcgcgtgtt cacgctgttt ccccggttgg cggagcggcg
     2101 cggccagctc gccggcaccc tctcgggcgg cgagcagcag atgctggcga tggcgcgggc
     2161 gctgatggcc cggccgcggc tgctcctgct cgacgagccc tcgatggggc tggccccggt
     2221 cctggtccgc gccatcttcc agacggtcgc cgcgatcaat cgggagggca cgacggtcct
     2281 gctcgtcgag cagaacgcga acgccgcgct gcgcctggct cggcgcgcct acgtgctcga
     2341 gaacggccgg atcgcgttag aggggctggg ctccgagctg gcaaaaaacg aagcggtgag
     2401 gcgggcgtat cttgggatcg gaacgcgcct gagcgctccg gaagg
//
LOCUS       DASQGH010000127         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31715, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000127 DASQGH010000000
VERSION     DASQGH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31715"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1556)
                     /locus_tag="VEW91_02450"
     CDS             complement(150..1556)
                     /locus_tag="VEW91_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYM90479.1"
                     /translation="MSDLAYASLAELSGRVRTRDLSPVALLDGLLERIERCRALHPFI
                     TVTAEVARAQATQAEREIAAGRYRGPLHGIPVSLKDLIDTKGIRTTCGSRILVDRVPA
                     ADATVAARLRAAGAILLGKNNLHEFAFGVTTANPHFGPTRNPWRPDRIPGGSSGGSGA
                     AVAVGCGPVSIGTDTGGSIRIPAALCGVVGLKPTYGRVSRHGVLPLSWTLDHVGPLTR
                     TVEDAALVLQAIAGPDPRDPSTLGQAVPEFTAGLRKPVAGLRVGVVADEFHSEMAPDV
                     RAPFRAALEVLATLGLELEDVEFPRAIEARTAAATVLFAEAASVHERWLPDRAGEYGA
                     DTLALLREGAFLTAAQYLRAQRVRALILREAGALMERLTALVLPTIPVVAPEIGQSTV
                     MLGGRPTDARAVLTRFVRHFNFVGLPAISVPCGFGADGLPVGLQIVGRSNDEPTVLAI
                     AHAYEQATPWHTRRPPEP"
     gene            complement(1553..1759)
                     /locus_tag="VEW91_02455"
     CDS             complement(1553..1759)
                     /locus_tag="VEW91_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90480.1"
                     /translation="MATVDDLARIAHLAGLSITRSDLERLAPILEALYADLDRLRKLP
                     IAELEPAFTPRPPDPAGRAGGGAP"
     gene            complement(1759..2979)
                     /locus_tag="VEW91_02460"
     CDS             complement(1759..2979)
                     /locus_tag="VEW91_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014511164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HYM90481.1"
                     /translation="MESAVDAVLEAVAEPYLLDLACRLVRTPSVYRPEDPGANETAVA
                     ALVAGELRRLGLEIHLEDAAPGRPNVIGDWIGPGVGPLLILEGHSDVVTEGDATMWSV
                     PPFGGIVADGRLYGRGAADMKGGIAAAIAAVRAIRDAGIALPGRIRIAVVADEEGMML
                     GIKSFIRNGWAREACGAIVCEPEANNVCLVQKGALRALARFRGRMAHGAMPKEGVNPI
                     LGSVAFIRQLERLEREYVARHGRHPLLGEPCVTPTVVRAGDLAQLNVIHAEAIVGVDM
                     RTIPGQDHAQIRRDLAAAVAAAVATVPGCAGDVEVIEERPCTETPGDAAIVEAVERAC
                     RRVQGRAPRRQGVPGATDGTFLHAWGKVPIVTIGPGDVTIPHQIDEFVRVADLVEASR
                     IFAAAACYCLGGAA"
     gene            complement(3012..>4278)
                     /locus_tag="VEW91_02465"
     CDS             complement(3012..>4278)
                     /locus_tag="VEW91_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYM90482.1"
                     /translation="RAAELARARLDELATLLTREEGKILSEARGEVLKGINLLEWYSG
                     EGFRMGGKTRPSEMPTTILFSLRQPLGVVAIITPWNFPWAEPVWKAAPALVAGNAVLL
                     KPASLTPLVPQRYLEILEEAGLPKGVLSIVVGAGPEVGDALVTHPAVRAVSFTGSCEI
                     GGEVYARAARRLAKVTCEMGGKNAVVVMPDADLELAAAGIAQGAFGSTGQRCTATSLC
                     VVHKDVKSRLVDAIVGRARAIRVGNGLEPGVGMGPLVDDRQLATVLDYIDGGRRAGAR
                     LACGGRRLTDGAYAGGYFIEPTVFTDVQPGHRIAQEEIFGPVLAVIDVGSLDEALRVT
                     NGLRYGLSSSIYAADANAIMQFVDNVEVGMVHVNSPTVGGEAQIPFGGIKWSGVGGRE
                     MAEEGLEFFTELKSVFWDYTGRARQTNIY"
BASE COUNT          612 a         1599 c         1485 g          582 t
ORIGIN      
        1 cgtcctcttc gctacgatcg ctccgagtga actggtgctg ctgctcctcg gggtgccgct
       61 gatgcacccg gtgcacgcgg ccctgcgccg ctttcggccg gtgcgcgacc ggccctgagg
      121 gccggcgcac cgctcgatcc cgcgggggcc tacggctccg gcggacgccg ggtgtgccac
      181 ggcgtcgcct gctcgtaggc gtgcgcgatc gccagcacgg tgggctcgtc gttcgaccgc
      241 cccacgatct gcagccccac cggcagcccg tcggccccaa acccgcacgg cacggagatc
      301 gccggaagcc ccacgaagtt gaaatgccgg acgaagcggg tgaggacggc tctcgcgtct
      361 gtggggcgcc ccccgagcat cacggtgctc tggccgatct ccggggccac gaccggaatc
      421 gtcgggagca ccagtgcggt gagccgctcc atcagcgcgc ccgcctcgcg caggatcaag
      481 gcccggacgc gctgcgcccg gaggtactgg gcggcggtga ggaacgctcc ctcccggagg
      541 agagcgagcg tgtcggcgcc gtactcgccg gcgcggtcgg gcagccaccg ctcatggacg
      601 ctggcggcct ccgcaaagag caccgtggcg gcggcggtcc gggcctcgat cgcccgtgga
      661 aactccacgt cctccagttc gaggcccaag gtggccagca cctcgagcgc cgcgcgaaac
      721 ggcgcgcgca catcgggggc catctcgcta tgaaactcat cggccaccac gccgacgcgc
      781 aggccggcca ccggctttcg gagtccggcg gtgaactccg gcaccgcctg gccgagcgtc
      841 gacgggtccc ggggatcggg gccggcgatg gcctggagga ccagcgccgc gtcctcgacg
      901 gtgcgggtga gcgggccgac gtggtcgagg gtccaggaga gcggaagcac cccgtggcgg
      961 ctgacacgcc cgtaggtcgg cttcagcccc accacgccgc acagcgccgc cgggatgcgg
     1021 atgctgcccc cggtgtcggt cccgatcgac accggcccgc atccgacggc caccgcggcg
     1081 ccggagccgc cgctggatcc gccggggatc cggtcggggc gccaggggtt gcgggtcggt
     1141 ccgaagtgcg ggttggcggt ggtgaccccg aaggcaaact catggagatt gttcttgccg
     1201 aggaggatcg cgccggcggc ccgaagccgc gcggcgaccg tggcgtcggc ggcgggcacg
     1261 cggtcgacca gaatgcgcga gccacacgtc gtcctgatcc ccttcgtatc gatgaggtcc
     1321 ttgagcgaga ccgggatgcc gtggagcggg ccgcggtatc ggccggcggc gatctcccgc
     1381 tcggcctggg tggcctgagc ccgcgccacc tccgcggtca cggtgataaa gggatgcagg
     1441 gcccggcacc gctcgatccg ttccaggagg ccatcgagga gggcgaccgg agacagatcg
     1501 cgggtgcgga cccggcccga cagctcggcc agcgacgcgt aggccagatc gctcacgggg
     1561 caccgccgcc cgcacgaccc gcgggatccg gcggccgggg cgtgaacgcc ggctccagct
     1621 ccgcgatggg gagcttcctc agccggtcca gatcggcata cagcgcctcg aggatcggcg
     1681 cgagccgctc caggtcgctt cgggtgatgg acagcccggc gagatgcgcg atccgcgcaa
     1741 ggtcatcgac cgtggccatc aggcggctcc accgaggcag tagcaggccg ccgccgcgaa
     1801 gatgcgggag gcctcgacca gatcggcgac ccgcacgaac tcgtcgatct ggtggggaat
     1861 ggtcacgtcc cccggcccga tcgtcacgat cggcaccttc ccccacgcgt gcaggaaggt
     1921 cccgtccgtc gctccgggaa ccccctggcg gcggggcgcc cgcccctgga cgcgccggca
     1981 tgcgcgctcg accgcctcca cgatggccgc atcccccggg gtctccgtgc agggccgctc
     2041 ttcgatcacc tcgacgtcac ccgcgcatcc ggggacagtg gccacggccg ccgccaccgc
     2101 cgcggcgaga tcccggcgga tctgcgcgtg atcctggccc ggaatcgttc gcatgtcgac
     2161 gcccacgatc gcctcggcgt gaatgacgtt gagctgcgcc aggtctcccg cccgcaccac
     2221 cgtcggcgtc acgcacggct cgccgagcag cggatgcctc ccgtggcggg cgacgtactc
     2281 gcgctcaagg cgctctagct gcctgatgaa cgcgacggat cccagaatcg ggttgacgcc
     2341 ctccttcggc atcgcgccgt gggccatccg accccggaat cgggcgagcg cgcgaagcgc
     2401 gcccttctgg acgaggcaga cgttgttggc ctccggttcg cagacgatgg ccccgcacgc
     2461 ctcccgggcc catccgttcc ggatgaacga cttgatgccg agcatcatcc cctcctcgtc
     2521 cgccaccacg gcgatccgga tccgcccagg gagcgcgatc cccgcatccc gaatcgcgcg
     2581 caccgccgcg atcgcggccg cgataccgcc cttcatgtcg gcggcgccgc gcccgtagag
     2641 gcgcccatcg gccacgatcc cgccaaacgg cgggacggac cacatcgtgg cgtccccctc
     2701 ggtcacgaca tcgctgtgcc cctcgaggat caggagcggg cccacccccg gtccgatcca
     2761 gtcgccgatc acgttcggcc ggccgggcgc cgcatcctcg aggtggatct ccagccccag
     2821 ccggcgcagc tcaccggcca ccagcgcggc caccgctgtc tcgttcgcgc cggggtcttc
     2881 ggggcggtag acgctcgggg tccgcacgag ccggcaggcc aggtcgagca ggtacggctc
     2941 ggccaccgcc tccaggacgg cgtcaactgc ggactccacc gcgcgcatcg tccgctgcgg
     3001 tcagcctgcg ttcagtagat gttggtctgc cgggcccggc cggtgtaatc ccaaaagacg
     3061 ctcttgagct cggtgaagaa ctccagccct tcctcggcca tctcccggcc accgaccccg
     3121 ctccacttga tgccgccgaa ggggatctgc gcttcgccgc ccacggtggg ggagttcacg
     3181 tgcaccatcc cgacctcgac gttgtcgaca aactgcatga tggcgttcgc gtcggcggcg
     3241 tagatcgacg aggagaggcc gtagcggagg ccgttggtca cccggagcgc ctcgtcgagc
     3301 gagccgacgt caatcaccgc gagcacgggc ccgaagatct cctcctgggc gatccggtgt
     3361 ccgggctgga cgtccgtgaa caccgtgggc tcgatgaaat agccgcccgc gtatgccccg
     3421 tcggtcagcc gccggccccc gcacgccagg cgcgctcccg cgcgccggcc gccgtcgatg
     3481 tagtcgagca ccgtggcgag ctgcctgtcg tccacgaggg ggcccatgcc gaccccgggc
     3541 tccagcccgt tgcccacgcg gatcgcgcgg gcccgcccca cgatcgcgtc gacgagccgg
     3601 ctcttgacgt ccttgtggac tacgcacagg ctggtggcgg tgcagcgctg gccggtgctc
     3661 ccgaacgcgc cctgggcgat gccggccgcg gccagctcca gatcggcgtc gggcatcacg
     3721 acgacggcgt ttttcccccc catctcgcag gtcacctttg cgagccggcg cgccgcgcgg
     3781 gcatagacct cgccgccgat ctcgcaggat cccgtgaacg agaccgcccg caccgcggga
     3841 tgggtgacga gggcgtcgcc gacctccggc cccgccccca cgacgatact gagcaccccc
     3901 ttgggaaggc cggcctcctc caggatctcc aggtagcgct gggggacgag cggggtgagc
     3961 gaggcgggct tgagcagcac ggcgttcccg gccaccagcg caggcgcggc cttccacacc
     4021 ggctcggccc acggaaaatt ccaaggcgtg ataatggcca cgacccccag gggctgccgg
     4081 agggagaaca ggatggtggt gggcatctcg gacgggcggg tcttgccacc catccgaaac
     4141 ccctcgccgg agtaccactc gagcaggttg atgcccttga gcacttctcc cctggcctcg
     4201 ctgaggatct tgccttcctc gcgggtcagc agcgtggcca gctcgtccag acgggcgcgg
     4261 gcgagctccg ccgcccgc
//
LOCUS       DASQGH010000128         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45892, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000128 DASQGH010000000
VERSION     DASQGH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45892"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VEW91_02470"
     CDS             complement(<1..1623)
                     /locus_tag="VEW91_02470"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90483.1"
                     /translation="MEIHTDYTDLLRANADACRSPHAIVRDLHRRRVSALSEVHASGG
                     LTNSRLIFDLSYHPERRPRRRWWKPVDGATYQRHLAELNILRDMMVGHCERCGFRARP
                     LDDEAMLAVAWRYFNPGRKRWCPPPGVPTENQAFEIPRRLLKAWPFFGHRSVRTAVAR
                     SDLYRRWDFLWLDGHAHAVVAMDTLPDRPTIPNVLGPLQTLPGAYWLIIEAWNDPRSD
                     QLKRLELKSRLSRQAVLAQDGGDSNARAVDRQVTDALDVIQQTSERVVRVGVAVVLQE
                     SSAEAVRAAARRALDTFHQIPGVDARIESVALRRQFLQLAPFSGMPNERLLRMFTSNA
                     ADFVPCHALWRGSTRPVCPFFTRRDSLVPLDPFDPRLPSWNAIVIGESGGGKTHFAIA
                     WLSHLLALDPVVLIVDKGGAYKAFCSVYGAQYIHIDPDADIAVNPFDLLPGETEPDHA
                     HLGLLKSLVALMVAEPDAALGRQEMAVLESAIRQTYARAAGSPVFLHDLVRTLRTFEQ
                     VGAHEAGPEEWTLARGIARRLELWVDEGLYARL"
BASE COUNT          310 a          543 c          566 g          270 t
ORIGIN      
        1 cagccgcgcg tacaaccctt cgtccaccca caactcgagg cggcgggcga tccccctggc
       61 cagggtccat tcttcgggtc ccgcctcgtg cgcaccgacc tgctcgaacg tgcgcagggt
      121 gcggacaaga tcgtgcaaga acacggggct accggcggca cgcgcatagg tctgccggat
      181 cgccgactca agcaccgcca tctcctggcg tcccagggca gcgtcgggct cggccaccat
      241 gagggcaacg agactcttga gcagcccgag atgggcgtga tcgggctcgg tctctccggg
      301 caggaggtcg aacgggttca ccgcgatgtc ggcgtccggg tcgatgtgga tgtattgggc
      361 gccgtacacg ctgcagaatg ccttgtaggc tccgcccttg tcgacgatca acaccacagg
      421 atcgagcgcc aataggtgcg acaaccaggc aatcgcgaag tgcgtcttgc ctcccccgga
      481 ttcgccgatc acgatggcat tccaggatgg gagacggggg tcgaagggat ccagaggtac
      541 cagactgtcc cgtcgcgtga agaatgggca gacaggacgt gtggaccccc gccacagcgc
      601 atgacacggg acgaagtccg ctgcgttgct agtgaacatc cgtaggagcc gctcgttggg
      661 catgccgctg aacggtgcga gttgtaggaa ctgccgccgg agggccacgg attcaattcg
      721 agcgtcgacg ccggggatct ggtgaaaagt atccagtgcc cgccgggcgg cggcgcggac
      781 ggcttccgcg ctcgattcct ggagaacgac ggcgactccg acgcgcacga cccgttcact
      841 agtctgctgg atcacatcca gggcatcggt gacctggcgg tccaccgcgc gggcgttcga
      901 gtctccgcca tcctgcgcga ggaccgcctg gcggctcaac cggctcttca actccaggcg
      961 tttgagctga tcggagcgcg ggtcattcca ggcctcgatg atcagccaat aggcaccggg
     1021 gagcgtctgc aacggcccca ggacattggg gatcgtgggc cggtccggga gcgtatccat
     1081 ggcgacgacc gcgtgcgcat gcccatccaa ccacaggaaa tcccaccgcc ggtacagatc
     1141 ggaccgggcc acggccgtcc ggaccgaccg gtggccgaag aacggccaag ccttgaggag
     1201 acggcgtggg atctcgaagg cctgattctc tgtgggaacg ccggggggcg ggcaccaccg
     1261 cttgcgaccg gggttgaagt atcgccaggc aacggccagc atcgcctcgt cgtcgagggg
     1321 acgtgcccga aacccgcagc gctcgcagtg ccccaccatc atatcgcgaa ggatgttgag
     1381 ttccgccagg tgccgttgat aggtggcccc atcgaccggc ttccaccaac gccgccgcgg
     1441 acgccgctca ggatggtatg acaggtcgaa gatcaggcgc gagttcgtga ggccgcccga
     1501 agcatgaacc tcgctcagtg cggagacccg gcgtcggtgc agatcccgga caatcgcatg
     1561 ggggctccgg catgcgtcgg cgttggcacg cagcaggtcg gtgtaatcgg tgtggatctc
     1621 caccagtatc cggaggcatt ccccctccgg cactgccgtt ccgatgagga tccgcaaacg
     1681 ttcgttgca
//
LOCUS       DASQGH010000129         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54284, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000129 DASQGH010000000
VERSION     DASQGH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54284"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1641
                     /locus_tag="VEW91_02475"
     CDS             292..1641
                     /locus_tag="VEW91_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYM90484.1"
                     /translation="MGYRWPLVDARKPVDLVEVSEPNLMREQFPYHHPPKVLFDGLEV
                     PLDPPEEILITDTTFRDGQQARAPLEVPQIVRLYDLLHRLSGPNGVIRKSEFFLYSTR
                     DREAVHQVLSRGYRYPEVTGWVRAKREDLRLVREVGLKETGILTSCSDYHIFLKLGWD
                     RRKAFENYVGVVSDALSQGLRVRCHLEDLTRADVYGFVVPLAQRLMTLGEEARIPVIL
                     RLCDTMGYGVPYPGAVLPRSVPRLVHAMIHDAGVPREWLEWHGHNDFYKVLINASAAW
                     LYGCAAANGTLLGYGERTGNTPIEGLVFEYAGLVGDLNGMDTTAIAEVAEYMERECQY
                     PIPASQPFVGRAFNATSAGIHADGAMKNEEIYNIFDTATLLNRPLAVNVTDRSGVAGV
                     AFWIRQHTGQSLPKDHPVVEAIHAWVVQQYSAGRTTAIVDEEMAEQVKRHLQKVTQP"
     gene            1810..>2848
                     /locus_tag="VEW91_02480"
     CDS             1810..>2848
                     /locus_tag="VEW91_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90485.1"
                     /translation="MDLRPAEIAMRQFSRALFGADPEEVKGFLAEAAATLERVNTELT
                     RVRLERTELQTEFQNSKKQAEAEIEELRRQLVAAQEKIAAHQKQESQMAQALMNAQRV
                     TDDLIQSSKARADRIVAEAKAASQVTMEAARREAAEGKAAALASAEATRKEAVELKAA
                     AQAALEAARKEAADLLRATQAQAQEAVRAADRAAEARMAQIQRESERLAEQARKEVSE
                     LHQTAVQQVERFVVNMKAFLSNWEGLSRNLEVLARSHAGALETIAQTRAEVESGVLPV
                     LRGLLHGLSAKGGTALREGVAAAATLDLSPFAPPALPPAPIKPAAAAPPSAPFKPGPA
                     PFKPGPAPSKPA"
BASE COUNT          483 a          940 c          976 g          449 t
ORIGIN      
        1 caaccgcagc cccgcgacct cttccccctt cgctttgagg atctcttccg tcacacggac
       61 ggcgtgcggc aggtaccctc agcggttgca gtactcgatc gcgatttcga gctttcccgc
      121 catcggcgat acctcccctg gacgtcttcc agcctccagt atcgcccgcc ggcggttcga
      181 tgtccaggga tcggccgcac acgagtcctc gcacgggccg gtaatattgc gcctgcaggg
      241 gccctatggt acgctcaaat tgagttcgaa gggttcgctc gggaggccgc cgtggggtat
      301 cgttggccgc ttgtcgacgc gcggaagccc gttgatctcg tggaggtcag cgagccgaac
      361 ctgatgcgcg agcagtttcc gtaccaccac ccccccaaag tgctttttga cggcctggag
      421 gtgcccctgg atccccccga ggagattctc atcaccgaca cgacgtttcg cgacgggcag
      481 caggcccgcg ccccgctcga ggtgccccag atcgtccggt tgtacgacct gctccaccgc
      541 ctcagcggcc ccaacggggt catccgcaag agcgagttct tcctctatag cacgcgcgat
      601 cgggaggcgg tccaccaggt cctctcccgg ggctatcggt atccggaagt gacagggtgg
      661 gtgcgggcca agcgggagga cctgcgattg gtgcgcgagg tggggctcaa ggaaaccggg
      721 attctcacgt cgtgctccga ctaccacatc tttctcaagc ttgggtggga ccggcgcaag
      781 gcctttgaga actatgtggg cgtggtctcg gacgcgctgt cgcagggcct ccgggtgcgc
      841 tgccacctcg aggacctgac ccgcgcagac gtctacggat tcgtcgtgcc cctggcccag
      901 cggttgatga ccctcggcga ggaggcgcgg attccggtca tcctacgcct ttgcgacacg
      961 atgggatacg gggtgcccta tccgggcgcg gtgcttcctc gcagcgtgcc gcgcctggtg
     1021 catgcgatga tccacgacgc cggggttccc cgggagtggc tggagtggca cgggcacaac
     1081 gacttctaca aggtcctgat caacgccagc gccgcctggc tctacggatg cgccgcggcc
     1141 aacggcaccc tgctcgggta cggggagcgg acgggcaaca cgccgatcga ggggctcgtc
     1201 ttcgagtacg cggggttggt tggcgacctc aacggcatgg acacgacggc catcgcggag
     1261 gtcgcggagt atatggagcg cgagtgccag tatccgatcc cggcgtccca gccatttgtc
     1321 ggccgggcct tcaacgcaac ctccgctgga atccacgcgg atggggcgat gaagaacgag
     1381 gaaatctaca atatctttga cacggcgacc ctgctcaacc ggccgctggc ggtcaacgtg
     1441 accgaccggt cgggtgtcgc cggggtggca ttctggatcc gccagcacac cggccagagt
     1501 ctcccgaagg atcacccggt ggtcgaggcg attcacgcgt gggtcgtgca gcaatattcg
     1561 gcggggcgga cgacggcgat cgtggacgag gagatggctg agcaggtgaa gcggcacctg
     1621 cagaaggtca cgcagccgtg aaatgcgcct gcgcgcaagc ccgtgcccgg ccggcttctt
     1681 tggggggggt tccgctccgg gcctttccga cctccgccgg cggcccctca ggcttgagcc
     1741 ggcgggtcta ctcccctccg cggcggggct tttgataggt gcgtcccctg gtgaccaaag
     1801 gagtgataga tggacctgcg gccggctgag atcgccatgc ggcagttctc tcgggccctt
     1861 tttggggctg accccgagga ggtcaaggga ttcttggccg aggccgccgc gacccttgaa
     1921 cgggtcaaca ccgagctgac ccgcgtccgc ctggagcgga cagagctcca gacagagttc
     1981 cagaacagca agaagcaggc agaggccgag atcgaggaac tacggcgcca actggtggcc
     2041 gctcaggaga agatcgcggc acaccagaag caggaaagcc agatggcgca ggcgctgatg
     2101 aacgcccaga gggtgaccga tgacctgatt cagagcagca aggcgcgggc cgatcggatc
     2161 gtggccgagg cgaaagccgc gtcgcaggtg accatggagg cggcgcgcag agaggccgcg
     2221 gagggcaaag ccgccgcgct ggcgagcgcg gaggcaaccc gcaaagaagc cgtcgagctc
     2281 aaagccgccg cccaggcggc cttggaggcg gcccgcaagg aggccgccga cctgcttcga
     2341 gccacccagg cgcaggcgca ggaggcggtt cgggccgcgg accgggccgc agaagcccgc
     2401 atggcgcaga tccagcgcga gtccgagcgc ctggcggagc aggcccgcaa ggaggttagc
     2461 gagctccacc agaccgcggt gcagcaggtc gagcgctttg tcgtcaatat gaaagccttt
     2521 ctatcgaact gggagggact ctctcgaaac ctcgaggttc tcgcccggag ccacgccggc
     2581 gcgctggaaa ccatcgccca gacgcgggcg gaggtggaga gcggcgtcct tccggtgctg
     2641 cgcggcttgt tgcacggcct gagcgccaaa ggggggaccg cccttcgcga gggggtcgcg
     2701 gcagccgcaa ccctggatct ttcccccttt gcgccgccgg ccctcccgcc cgcgccgatc
     2761 aagcctgcgg ctgcggcccc gccgtccgca ccgttcaagc ctggccccgc accgttcaag
     2821 cctggccccg ccccgtccaa gcctgccg
//
LOCUS       DASQGH010000130         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11902, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000130 DASQGH010000000
VERSION     DASQGH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11902"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VEW91_02485"
     CDS             complement(<1..337)
                     /locus_tag="VEW91_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90486.1"
                     /translation="MAAGPVRGFPDRAQALAALLAYAALSVAFFGFPILRDPAHVYIG
                     RGGDATLMIWFLAWWPYALGHGLNPLVTHLVWAPTGVNLGWTTAIPGAGILAAPITLA
                     FGPVVAYNFL"
     gene            complement(437..1654)
                     /locus_tag="VEW91_02490"
     CDS             complement(437..1654)
                     /locus_tag="VEW91_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYM90487.1"
                     /translation="MAVYEYTARDATGKVLAGAIEADTDATVTQKLREMGFFITSLQR
                     KNQAVGVGEYFARFKGIGLKDLAIFSRQFATMVNAGLSLVRTLSILEEQTTQRQLRAI
                     IGEVRTDIEGGATLSAALGKHPRAFSNLYVNMVKAGEAGGVLDEVLVRLAAFLEKELA
                     LRQKIKSATTYPILLACAAIAALLFMTVVIIPQFATFFKELGSNAALPLPTQIAMGVS
                     ILIRRFWWMMILAMVPIVYLTRLYVRTPAGRASYDQLKLRLPILGPVNKKIVIARFSR
                     TFGTLVASGVPIMQALEVVGKAIDNTVLARAVESVRSSIREGESIAIPLAASGMFPPM
                     VVQMVKVGEETGALETMLTKVADFYDTEIEATVAGLTSILEPVLIVGMGIVIGAMLIS
                     LYLPIFNLALSVK"
BASE COUNT          320 a          618 c          619 g          296 t
ORIGIN      
        1 agaggaagtt gtaggccacc accgggccga acgccagcgt gatgggggcg gcgaggatgc
       61 ccgctccggg aatcgccgtg gtccagccca gattcacgcc ggtgggcgcc cacacgaggt
      121 gggtgaccag agggttcaac ccgtgcccaa gcgcataggg ccaccacgcc aggaaccaga
      181 tcatgagcgt cgcgtcaccg ccccggccga tgtagacgtg ggcgggatcc cgcaagatgg
      241 ggaagccgaa gaaggcgacc gaaagggcgg cgtacgcgag tagcgcagcg agcgcctgag
      301 cgcggtcggg gaagccacgc accggaccgg ccgccatcaa ctcgatgctc ccttgcggga
      361 tctcaacgcg gccgcggcgt ccctttccga cgccgcggcg cgcgcgaacc cgcgggggcg
      421 tgtggcccga gagggcctac ttcaccgaaa gcgccaggtt gaagatcggc aggtacagcg
      481 agatcagcat ggcgccgatg acgatcccca tccccacgat gaggacgggc tcgaggatcg
      541 acgtgaggcc ggcgaccgtg gcctcgatct cggtgtcgta gaagtccgcc accttcgtca
      601 gcatggtctc gagcgccccg gtctcctccc ccaccttcac catctgcacc accatggggg
      661 gaaacatgcc gcttgccgcg agcgggatcg cgatgctctc gccctcacgg atgctcgagc
      721 gcaccgattc gaccgcgcgg gcgaggaccg tgttatcgat ggccttgccc acgacctcga
      781 gggcctgcat gatcggcacg ccgctcgcca ccagggtgcc gaaggtgcgg ctgaaccggg
      841 caatgacgat cttcttgttg accggcccca ggatcggcag ccgcagcttg agctggtcgt
      901 agctggcccg cccggccggg gtccgcacat acaaccgggt cagatagacg atcggcacca
      961 tcgcgaggat catcatccac cagaagcggc ggatcaggat gctcaccccc atcgcgatct
     1021 gggtcgggag gggcagcgcg gcgttcgagc cgagctcctt gaagaaggtc gcaaactgcg
     1081 ggatgatcac gaccgtcatg aagagcagcg cggcgatcgc ggcgcaggcc agcaggatcg
     1141 ggtacgtcgt cgccgacttg atcttctgcc gcagcgccag ttccttctcg aggaacgcgg
     1201 ccaggcgaac caggacctcg tcgagcacgc cgccggcctc gcccgctttc accatgttca
     1261 cgtagaggtt gctgaacgct ctgggatgct tgccgagcgc ggcgctcagg gtggcgccgc
     1321 cttcgatgtc ggtccgcacc tcgccgatga tggcccggag ctgccgctgc gtcgtctgct
     1381 cctcgaggat cgagagcgtc cgcacgagcg acaacccggc gttcaccatg gtggcgaact
     1441 gccggctgaa gatggccagg tctttgaggc cgatcccctt gaaccgggcg aagtattcac
     1501 cgacccccac cgcctggttc ttgcgctgga gactggtgat gaagaacccc atctcccgca
     1561 gcttctgggt gacggtggca tcggtgtccg cctcgatggc ccccgcgagc actttcccgg
     1621 tcgcgtcccg ggcggtgtac tcatagactg ccatggcgct tcccctccgt cggagtcgta
     1681 ggtgtcctgg gcatgagcat cactcggcga tcagctttcg caggtcgtca gggtggatcg
     1741 cgcgcccgat ggcgtcctcg taactgatca ggcggcgacg gtagaggtcg gccagcgaca
     1801 tatccatcgt ctgcatcccg tactgggccc cggtctgcag cgtggactcg atc
//
LOCUS       DASQGH010000131         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3351, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000131 DASQGH010000000
VERSION     DASQGH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3351"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1386)
                     /locus_tag="VEW91_02495"
     CDS             complement(136..1386)
                     /locus_tag="VEW91_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYM90488.1"
                     /translation="MNMALVSELELPAFDYTDESMRGDRFHRAMEELRATGWLAEGPY
                     GYVALDRESAEFFLRTRSAIFPGMKIAEIFEVGEGPLYEQMRRNILHVNGPDHTRLRS
                     LVNPALSARAVERYRPAMRGFLEELLERAVAASASDGIVRCEFVEAFAKPYPSLVIAE
                     VMGAPLQDAPRLHHWSNWIQRQFDAVSMAEEREGIERAVEEFYEYAEALLRARRKNPG
                     DDLISKLIAAEEEGDRLSDVECINLVFNVLVGGVDTSQSQLAHAIRLLAENPGQWALL
                     VEDPSLAGQAVEEALRYEPITPFTARILTEEVSYRDVVFPEGTIVMVCAFTGNRDLGP
                     DQRGEGGPERFDIAADRGRARALTFGAGVHYCLGANLARLELQEGLAFLAERIGGLEL
                     DGEPRFGTISGIYGLEELPIRFSV"
     gene            complement(1400..>2108)
                     /locus_tag="VEW91_02500"
     CDS             complement(1400..>2108)
                     /locus_tag="VEW91_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYM90489.1"
                     /translation="EGCGAMTDIFAAGLRPSALDFLDGETLGMVAGAYPGEAPARAGF
                     ALIVEVDGAQAEAEAQRGELLGLLGPAAVAVDEPADVAALWRWRDGVNPAVTAVRGAK
                     VSEDVVFPIERLAEGLEGFEEIASRHGLRSCAWGHGGEGNVHATVLVDPDREAELDAA
                     EAVGEELFALVVSLGGSIAGEHGVGWMKRGRLEAQWDRRAVELHEQIKRAFDPKGLLN
                     PGTKLARYPARSPATST"
BASE COUNT          299 a          881 c          618 g          310 t
ORIGIN      
        1 cgcgatggtc ggggcgaggg cgaggaggac cgctgccttg aatatccgta gggccatctc
       61 aaccaggtgt cgctcgggga gccgtcatgg cccccctccc agattctcgc atcctcgccg
      121 tggcagcgcc ggccgtcaga cggagaacct gatcggcagc tcctccaggc cgtagatccc
      181 cgagatggtt ccgaaccggg gctcgccgtc gagctccagg ccgccgatgc gctcggcgag
      241 gaacgcgagc ccctcctgca gctccagccg cgccaggttc gcccccaggc agtagtggac
      301 gcccgccccg aacgtcaacg cccgcgcccg gccccggtcg gccgcgatgt cgaagcgctc
      361 cggcccgccc tccccgcgct ggtccggccc gaggtcgcga ttgcccgtga aagcgcagac
      421 catcacgatc gtcccctccg ggaagacgac atcgcggtag gagacctcct cggtgaggat
      481 cctcgcggtg aacggggtga tcggctcata cctcagcgcc tcctccaccg cctgccccgc
      541 aagcgagggg tcctccacca gcagcgccca ctgcccgggg ttctccgcca gcagcctgat
      601 ggcatgcgcg agctggctct gactcgtgtc gacgcccccc accagcacgt tgaacacgag
      661 gttgatgcac tccacgtcgc tcagccgatc gccttcctcc tccgccgcga tcagcttcga
      721 gatcaggtcg tcccctgggt tctttcgccg cgctcgcagc agcgcctcgg cgtactcgta
      781 gaactcctcc accgcccgct ctatcccctc gcgctcctcc gccatgctca ccgcgtcgaa
      841 ctgccgctgg atccagttcg accagtggtg cagcctcggc gcgtcctgca gcggtgcccc
      901 catcacctcc gcgatcacca gcgatggata gggcttcgcg aacgcctcca cgaactcgca
      961 cctgacgatc ccgtcggatg cggaagcggc caccgcccgc tccagcagct cctccagaaa
     1021 gccccgcatg gccggccggt agcgctccac cgcccgcgcc gacagggccg ggttcacaag
     1081 gctgcgcagg cgcgtgtggt ccgggccgtt cacgtgcagg atgttccgcc tcatctgctc
     1141 gtacagcggg ccctcgccca cctcgaagat ttccgcgatc ttcatccccg ggaagatcgc
     1201 cgaccgcgtg cgcaggaaga actccgccga ctcccggtcg agggccacgt acccgtacgg
     1261 cccctcggcc agccagcccg tcgcccgcag ttcctccatc gcccggtgga agcggtcccc
     1321 gcgcatcgac tcgtcggtgt agtcgaaggc ggggagctcc agctcgctga cgagcgccat
     1381 gttcatatgg agcttccttt caggtggatg tggccggcga gcgggcaggg taccgtgcca
     1441 gcttcgtccc cgggttgagc aggcccttgg ggtcgaacgc ccgcttgatc tgctcgtgca
     1501 gctccaccgc ccgccgatcc cactgggcct ccaggcgacc gcgcttcatc cagccgacgc
     1561 cgtgctcgcc ggcgatcgac cccccgagcg agaccaccag cgcgaacagc tcctccccga
     1621 ccgcctcagc cgcgtcgagc tccgcctccc tgtcggggtc caccagcacc gtcgcgtgca
     1681 cgttcccctc gccaccatgc ccccacgcgc acgaccgcag gccgtggcgg ctcgcgatct
     1741 cctcgaagcc ctccaggccc tccgccagcc gctcgatcgg gaacaccacg tcctcgctca
     1801 ccttcgcccc ccgcaccgcc gtcaccgccg ggttgacccc gtcgcgccag cgccacagcg
     1861 ccgccacgtc cgccggctcg tccaccgcca ccgccgccgg gccgagcagc cccagcagct
     1921 ccccccgctg ggcctccgcc tccgcctgcg cgccgtccac ctccactatc agcgcgaatc
     1981 ccgcccgcgc cggcgcctcg ccgggatagg cgcccgccac catccccagc gtctccccgt
     2041 cgaggaagtc gagcgccgac ggcctcaggc ccgccgcgaa gatgtccgtc atcgccccgc
     2101 acccctcc
//
LOCUS       DASQGH010000132         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3354, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000132 DASQGH010000000
VERSION     DASQGH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="holB"
                     /locus_tag="VEW91_02505"
     CDS             complement(<1..720)
                     /gene="holB"
                     /locus_tag="VEW91_02505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HYM90490.1"
                     /translation="MPFRDLIGQPHARLVLQGALRSGRISHAYLFVGPSGVGRLATAR
                     AFAQALLCAAGGEDACGVCGPCRKVAGGTHPDLRIIAPGRTESGAERRAVAIDQVRDL
                     KHDAAYPPYEARWKVFVIEDTEEMRAEAANSLLKLLEEPPASIVIILLSESTEALLPT
                     LVSRAQLVRFGPVPTHEIARALTERGVPPSRARFLAAMAGGRIGTALEAARAGEEPFA
                     RRAEVLATLRAVEGGDLIAGLD"
     gene            complement(738..1067)
                     /locus_tag="VEW91_02510"
     CDS             complement(738..1067)
                     /locus_tag="VEW91_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HYM90491.1"
                     /translation="MKLVLAIVQDKDTRGLMEALVAAEFQATKLASTGGFLREGNATI
                     LIGTEESKVEAVLAIIQKTCHVREQLVSPLPPVVEPVDSYISAPVKVLVGGAVVFVLD
                     VERMVKI"
     gene            complement(1064..>1577)
                     /gene="tmk"
                     /locus_tag="VEW91_02515"
     CDS             complement(1064..>1577)
                     /gene="tmk"
                     /locus_tag="VEW91_02515"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYM90492.1"
                     /translation="WEEMDARTEMLLFAAARAQLVAEVIAPALRAGKVVVCDRYVDAS
                     VVYQGIGRRLGVEIVRTVNAVATGGVSPDLTLLLDIDVRAGLDRARAETGMRIPAGEA
                     AAGDRIEQERLAFHERVREGFLSLAKNEPHRIKVIDAHGPVPTVQWAIRQAVAQTLHG
                     REGPGTEGSR"
BASE COUNT          233 a          584 c          529 g          231 t
ORIGIN      
        1 atccaggccg gcgatcaggt ccccgccctc gacagcccgg agcgtggcca gcacctcggc
       61 cctgcgggcg aacggttcct cccccgcgcg ggcggcctcc agcgcggtgc cgatccgacc
      121 gccggccatg gcggcgagga agcgggcgcg ggacggggga acgccccgct cggtcagggc
      181 gcgcgcgatc tcgtgggtgg gcaccggtcc aaagcgcacg agttgcgccc gggagaccag
      241 ggtgggcagg agcgcctcgg tggactcgga gagcaggatg atcacgatgc tcgccggggg
      301 ttcctccagg agcttcagga ggctgttggc cgcctccgcg cgcatctctt cagtgtcctc
      361 gatgacaaac accttccacc gggcttcata cgggggatac gccgcgtcgt gcttgaggtc
      421 tcggacctgg tcgatcgcga ccgcgcgccg ctccgcgccg gattcggttc gtcccggcgc
      481 gatgatgcgg aggtcgggat gcgttccccc cgccaccttc cggcacggtc cgcacacccc
      541 gcaggcgtcc tcgccccccg cggcgcacag caacgcctgg gcaaacgcac gggccgtcgc
      601 gaggcggccc acgccagagg gaccgacgaa gaggtaggca tgcgagatcc ggccgctgcg
      661 gagcgccccc tggagcacca ggcgggcgtg gggctggccg atcaggtcgc ggaacggcat
      721 cccaagggcg tgggatccta gatcttgacc atccgctcca cgtcgagcac gaacacgacg
      781 gcgccgccga ccagcacctt gaccggcgcc gagatgtagg agtcgacggg ttccaccacc
      841 ggcggcagcg ggctgacgag ctgctcccgg acgtggcagg tcttttggat gatcgcgaga
      901 accgcctcca ccttgctctc ttcggtgccg atcaggatcg tcgcgttgcc ttcgcggaga
      961 aacccgcccg tgctcgccag cttcgtcgcc tgaaactcgg ctgccaccag cgcttccatc
     1021 agcccccggg tgtccttgtc ctgcacgatc gcgaggacca gcttcatcgg gacccctccg
     1081 tgccgggccc ctcccggcca tgcagagtct gcgccacggc ctgtcggatc gcccattgga
     1141 cggtggggac cggcccgtgg gcatcgatca ccttgattcg gtggggctca ttcttggcaa
     1201 gcgaaagaaa cccctcccgg acccgctcgt ggaaggccag gcgctcctgc tcgatgcgat
     1261 ctcccgcggc tgcctcgccc gcggggatgc gcatcccggt ctcagcccgc gcccggtcga
     1321 gtcctgcccg cacatcgatg tcgagcagca gggtgaggtc ggggctcacc cccccggtgg
     1381 cgaccgcgtt gacggtccgg acgatctcca ccccaagcct ccgcccgatc ccctggtaga
     1441 cgaccgacgc gtccacgtaa cgatcgcaga ccaccacctt gcccgcccgg agggcggggg
     1501 cgatgacctc cgccaccagt tgggcccggg ccgccgcgaa taacagcatc tcggtgcggg
     1561 cgtccatctc ctcccac
//
LOCUS       DASQGH010000133         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31728, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000133 DASQGH010000000
VERSION     DASQGH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31728"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1560
                     /locus_tag="VEW91_02520"
     CDS             187..1560
                     /locus_tag="VEW91_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HYM90493.1"
                     /translation="MSEQSVQTVLFPTLFAKSLVAAFDTPQQSSDGGAILLKAIDAQL
                     GLTARLAACLPEWRQVGKVEHDLPTLIRQRCFGLACGYPDANDAARLKDDPIHKLLVG
                     RDPVAGPPLASQPTLSRFENAIATRDLVRLTHALADVVIAHHQARLGAQRVRRITIDL
                     DPTDDPTHGQQELAFFNGHYDTWCYLPVVGYLTFNKEREQYLVTALLRPGNVPASRGA
                     IGLLRRLFAKLRAAFPKATLRVRLDGGYATPPLFAFLEAEGVEYLVAMASNARLAKRI
                     RRLLGKARMRSKASGQTEHLYGETRYAAKKWRRKRRVIMKAEVVRLGDRDPRDNPRFV
                     VTNLPQTPRHVYALYCERGDRENRIKELHYGLGLDRTSCSRFRANAFRVVLTAAAYVL
                     LQELRRHLAATACAGSQVGTLRERLLKLGTRVSVSARRIVLHLPSAFPWLVVWQAVAL
                     ALGARAG"
BASE COUNT          295 a          669 c          595 g          295 t
ORIGIN      
        1 tccgcatccg tggcggaacg ttgaccggca tcttgagttg tcaggacgtc gaatccacgc
       61 tgcgtgagga tccgggcaag gagagggtct accctggaaa attcatgaag acgaggaaca
      121 aaagcgcctc catcgtggtt aagctctgtg aacgcgggca gttcccgtca cagaggaggc
      181 gcacccgtgt ccgaacagtc tgtgcagacc gtgctgttcc cgacgctgtt cgccaagtcc
      241 ctcgtcgcag ccttcgacac gccccagcag agctcggatg ggggagccat cctgctgaag
      301 gcgatcgacg cccagctcgg cctcaccgcc cggctcgcgg cctgcctccc ggagtggcgc
      361 caggtgggga aggtcgagca cgacctgccc accctcatcc ggcagcgctg cttcggcctg
      421 gcctgtggct accccgatgc caacgatgcg gcccgcctca aggacgatcc catccacaag
      481 ctgctcgtgg ggcgcgatcc ggtcgcgggc cctccgctgg cttcccagcc caccctgtcc
      541 cgcttcgaga acgccatcgc cacccgggac ctcgtgcggc tcacccacgc cctcgccgat
      601 gtggtcatcg cccaccacca ggcgcgcctc ggcgcgcagc gggtccggcg gattacgatc
      661 gacctcgatc cgacagacga cccgacccac gggcagcagg agctcgcctt cttcaacggc
      721 cactacgaca cgtggtgcta cctgcccgtg gtgggctacc tcaccttcaa caaggaacgc
      781 gaacagtacc tcgtcacggc gctcctccgt cccggcaatg tccccgcgag ccgaggcgcc
      841 atcggcctcc tgcggcgtct ctttgccaag ctccgggcgg ccttcccgaa ggccacgctc
      901 cgggtccggc tggatggggg ctacgccacg cccccgctct tcgccttcct cgaggcggag
      961 ggcgtggagt acctcgtggc catggccagc aacgcccgcc tggccaagcg gatccgccgc
     1021 ctcctgggca aggcccgcat gcgctccaag gccagtgggc agaccgagca cctctacggg
     1081 gagacccgct acgccgccaa gaagtggcgc cgcaagcgcc gggtgatcat gaaggccgag
     1141 gtcgtccgct tgggggaccg cgacccccgg gacaacccgc gcttcgtcgt gaccaacctc
     1201 ccccagaccc cgcggcacgt ctacgccctc tactgcgaac ggggcgaccg ggagaaccgg
     1261 atcaaggagt tgcattacgg cctcgggttg gaccggacga gctgctcccg gttccgcgcg
     1321 aacgccttcc gggtggtgct caccgcggcc gcctatgtct tactccagga gctccgccgg
     1381 catctggccg ccaccgcctg cgccggcagt caggtaggga cgctccggga gcgcctcctc
     1441 aagttgggca cgcgggtcag cgtctcggcc cggcggatcg tgctgcatct gcccagcgcc
     1501 ttcccctggc tcgtggtttg gcaggccgtg gcgctggcgc tcggagctcg cgccgggtaa
     1561 ccaccactgt cgcccgcttc gctgcagtcc gctccacagg agtcgtgtct ctgccgggac
     1621 ccccagcgtg gcagcgcgtg ccctgccggt ccgtctcgcc tcccgacaag ggtcgttccc
     1681 gcgtcttgat gacgcctgca aggcttggag cagcagtgat gggctgttca gcgagcatcg
     1741 atcgctcgtt caggaataat ccgggctaag aagtggcagg aggaggagcg aaaggttgag
     1801 agggacatcc gcgcgaggcg ggtgaaggtt tccaagagcg tcgaagcgct cctg
//
LOCUS       DASQGH010000134         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6265, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000134 DASQGH010000000
VERSION     DASQGH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6265"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..898
                     /gene="rlmB"
                     /locus_tag="VEW91_02525"
     CDS             101..898
                     /gene="rlmB"
                     /locus_tag="VEW91_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HYM90494.1"
                     /translation="MTPSPVPPGPALLGRRAVLEALRAGRPVSRVLVARTADLRGPLR
                     EIVQEARERRVIVETVDRRHLDALARGIVHQGVAALVAAAAPVAVEDLLQRAQDRGEA
                     PFLIALDGVQDPHNLGAVIRTADAAGAHGVIIPRRRAAGLGPAVARASAGAIAHLPVA
                     QAGNLVAVLDRLKAKGVWVVGADAAGSERYDTIGLAPPVVLVVGGEHRGLHRLVRERC
                     DRVVRIPLWGRVASLNVSVAAALLLYEVARHLTRSDAHRDGPRGQSC"
     gene            1092..1733
                     /gene="sigH"
                     /locus_tag="VEW91_02530"
     CDS             1092..1733
                     /gene="sigH"
                     /locus_tag="VEW91_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HYM90495.1"
                     /translation="MVVARKPVAPIYQEMVDEQLVDCAKGGDEFAAEYLINKYRNFVR
                     VKAKAYFLIGADREDIIQEGMIGLYKAIRDFRRDKLSSFRAFAELCITRQIITAIKTA
                     TRQKHIPLNSYISLNKPIYDEDSDRTLLDVISSIKVSDPEELVISQEASLTMRERIRK
                     NLSDLECRVLTAYLEGKSYQEMATELHRHVKSIDNALQRVKRKLERNLEGEEE"
     gene            complement(1742..>2370)
                     /locus_tag="VEW91_02535"
     CDS             complement(1742..>2370)
                     /locus_tag="VEW91_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYM90496.1"
                     /translation="SLDSLDRGKFKRITRWGNLDDVLAGLEAARTAGLRPVKINTVVV
                     RGFNEDDVVDLARLTLEHDYAVRFIEMMPFGSVAGFQTNAYVPSRETMARIEAVLGPL
                     TPLDVSGYDPARTYRVDGARGILGFISSVSQPFCAQCGRLRLTAEGRLRLCLLRDDEV
                     DLRTPLRAGATYEEIRERFRAAAYRKPWGHGLARGVIPRQRIMSQIGG"
BASE COUNT          389 a          816 c          805 g          360 t
ORIGIN      
        1 agcggaaaga ttggtcgacc ggtgacgcga tccggtcccg gctccacagc ctcggcatcc
       61 tgctcgagga taccccgacc ggctttacgt ggcggctgcg gtgaccccgt cgcctgtccc
      121 cccggggccg gcgctcctcg ggcgtcgggc ggtgctggag gcgctcaggg ccggccggcc
      181 ggtgtcgcgc gtcctggtgg cccggaccgc cgacctccgc ggccccctcc gggagatcgt
      241 ccaggaggcg cgcgagcggc gggtgatcgt ggagaccgtg gaccggcggc acctcgatgc
      301 gctggcccgc ggcatcgtcc accagggggt cgccgcgctg gtcgcggcgg ccgcgccggt
      361 tgcggtcgag gacctgttac agcgcgcgca ggaccgcggg gaagcaccgt tcctcatcgc
      421 gctggacggg gtccaggatc cccacaatct cggcgccgtg atccgcaccg ccgacgcggc
      481 cggggcccac ggggtcatca ttccgcggcg ccgggccgct ggactcggcc cggcggtggc
      541 ccgcgcctcc gccggcgcga tcgcccacct ccccgtggcg caggccggca acctggtcgc
      601 ggtgctcgac cgcctcaagg cgaagggggt gtgggtcgtg ggcgcggatg cggccgggtc
      661 cgaacggtac gatacgatcg gcctcgcccc cccggtggtg ctggtcgtgg ggggcgagca
      721 ccgcgggctc catcgtctcg tgcgcgagcg gtgcgatcgc gtggtccgca tcccgctttg
      781 gggccgggtg gcgtcgctca atgtctccgt ggccgccgcg ctgctcctct acgaggtggc
      841 gcggcatctc accagatccg atgcgcatcg cgacgggccc cgtgggcaat cttgttgact
      901 tgactcgaac attgtccata taataggact aacctgcggc agcggccaca agcggcgccc
      961 agcgggggac cggatgaacg tccgatacga tcgggcaatc gaccaccccg gcggtagcag
     1021 tcgatggaac agggtaggat gtttcagggg tcacgcgtag cagccgaaca agctggccgg
     1081 aggcgagaag cgtggtagtt gcgcgcaagc ccgtcgcacc gatctatcag gaaatggtcg
     1141 atgagcagct ggtcgattgt gccaagggcg gggatgagtt cgccgccgag tatctgatca
     1201 acaagtaccg caacttcgtc cgggtcaaag ccaaagccta tttcctgatc ggggccgatc
     1261 gcgaggacat catccaggaa gggatgatcg gcctctacaa ggccatccgg gacttccggc
     1321 gggacaagct gtcgtcgttc cgggccttcg ccgagttgtg catcacccgc cagatcatca
     1381 ccgcgatcaa aaccgcaacg cgccagaaac atatcccgct caactcgtac atctcgctca
     1441 acaagccgat ctacgatgag gactccgacc ggaccctcct cgacgtgatc agcagcatca
     1501 aggtctccga ccccgaggaa ctcgtcatca gccaggaagc ttcgctcacg atgcgggagc
     1561 gcatccggaa gaacttgagc gacctcgagt gccgggtgct gacggcctac ctggagggca
     1621 agtcctacca ggagatggcg accgaactcc accgccacgt gaagtccatc gacaacgccc
     1681 tgcagcgggt caagcgcaag ctcgaacgca acctcgaagg cgaagaagaa taaccccggc
     1741 gttagccgcc gatctgtgac ataatccgct gacgcgggat gaccccgcgc gccaggccgt
     1801 gcccccaggg tttgcggtac gcggcggcgc ggaagcgttc gcggatctcc tcgtacgtcg
     1861 ccccggcgcg tagcggcgtg cgcaggtcga cttcgtcgtc gcgcaggagg cacaaccgca
     1921 gccgcccctc ggccgtgagc ctcagccggc cgcactgggc gcagaagggc tggctcaccg
     1981 agctgatgaa cccgaggatg ccgcgcgcgc catccacgcg gtacgtccgc gcggggtcgt
     2041 agccgctcac gtcgagcggg gtgagcgggc cgagcaccgc ctcgatccgc gccatcgtct
     2101 cccggctcgg cacgtacgcg ttggtctgaa acccggccac gctcccgaac ggcatcatct
     2161 caatgaaccg cacggcgtag tcgtgctcca gggtcagcct ggccaggtcc accacgtcgt
     2221 cctcgttgaa cccgcggacc acgacggtat tgatcttgac ggggcgcagg ccggccgtcc
     2281 gggccgcctc cagcccggcc agcacgtcat cgaggttgcc ccagcgggtg attcgcttga
     2341 acttgccgcg gtcgaggctg tccaggctca
//
LOCUS       DASQGH010000135         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3373, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000135 DASQGH010000000
VERSION     DASQGH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VEW91_02540"
     CDS             <1..301
                     /locus_tag="VEW91_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYM90497.1"
                     /translation="RIHGAPVVDHSGGLVGMLSLVDLAGRLGTRVLHVMRTDLVTASE
                     DADVGHLASLMLAQRVRRIPIMRGSRLVGIVSASDLIQGLLELHEAAGVREEEKV"
     gene            317..1252
                     /gene="arcC"
                     /locus_tag="VEW91_02545"
     CDS             317..1252
                     /gene="arcC"
                     /locus_tag="VEW91_02545"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010874333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HYM90498.1"
                     /translation="MTGTRRRHVKRVIIALGGNALLRPGEPLEADVQRRNVRLAAHAI
                     AGVARAHQVVITHGNGPQVGLLALQSESYRDVHPYPLDVLGAESEGMIGYMIEQELVN
                     EVPGRDVLTLLTQVIVDITDPAFHLPSKPVGPVYSEEQAQRIAAERGWTVTRAGNGFR
                     RVVPSPEPKRIIELPAIRLLVEAGALVICAGGGGIPVAIDEAGAIRGVEAVIDKDLSA
                     ALLATHLQADVLLMLTDVSAVCTDWGTPQARPVRRATPSQLRRYRFEPGSMGPKVEAA
                     CRFVEATGGTAAIGALGDAAAILDGEAGTVVRLTG"
     gene            complement(1295..2617)
                     /locus_tag="VEW91_02550"
     CDS             complement(1295..2617)
                     /locus_tag="VEW91_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HYM90499.1"
                     /translation="MATTARRVLILGAAGRDFHNFNVCYRDDPRYAVVAFTAAQIPNI
                     AGRRYPPQLAGPRYPHGIPIYKEEELPELIRRLDVQEVVFAYSDVPHEHVMHLGSVAL
                     AGGADFVLLGPRRTMLEARKPVIAVCAVRTGAGKSPAARRVAAIIREAGRRPVVVRHP
                     MAYGNLADQACQRFASWEDLDRHRCTIEEREEYEPHLARGTVVYAGVDSHRVLAAAED
                     EADAIVWDGGNNDFPFFRPDLHIVIVDPHRAGDERRYHPGETNLLMADLVVVSKVNTA
                     SPEAVRAVRASAEGANPSALIVEADLAISVDHEELIAGRRVLVVEDGPTLTHGGMAYG
                     AGMLAAQQRGATLVDPRPWAPASLAAVYRRHPQLGPVLPAMGYSEEQLGTLTTTIAAV
                     PCDAVVCATPIDLRRLVRIERPVARVTYELQEHPENALVAPIRRTLRA"
BASE COUNT          395 a          867 c          991 g          411 t
ORIGIN      
        1 ccggattcac ggcgccccgg tggtggatca ttcggggggg ctcgtgggca tgctcagcct
       61 cgtcgatctc gcgggacggc ttggtacccg ggtgctgcat gtgatgcgca ccgacctcgt
      121 cacggcctcc gaggacgccg atgtggggca cctcgcctcg ttgatgctgg cccagcgcgt
      181 acggcgaatc ccgatcatgc gcggttcgcg gctggtgggt atcgtgagcg ccagtgacct
      241 gatccaaggg ctgctcgagc tccacgaggc cgccggcgtg cgcgaggagg aaaaggtcta
      301 agccccaggg gctgggatca ccgggacgag gcgccgacat gtgaagcggg tcatcattgc
      361 actgggcggg aacgccctgc ttcggccggg cgaacctctg gaggcggacg tacagcgccg
      421 gaacgtcagg ttggctgcgc acgcgatcgc cggcgtcgcc cgagcgcatc aggtcgttat
      481 cacgcacgga aacggcccgc aggtggggtt gctggccctg caaagcgagt cctaccggga
      541 cgtacacccc tatccgctcg atgtcctcgg cgcggagagc gagggcatga tcggctacat
      601 gatcgagcaa gagttggtca acgaggtgcc gggccgcgac gtcctcacgc tcctcaccca
      661 ggtgatcgtc gacatcaccg atccggcatt tcacctcccg agcaagccgg ttgggccggt
      721 ctattcggag gagcaggcgc agcgcatcgc cgcggaacgg gggtggacgg tgacgcgggc
      781 gggcaacggc ttccgccggg tggttccgtc gccggagccg aagcggatca tcgagttacc
      841 ggccatccgg ctgctcgtgg aggccggtgc cctcgtcatc tgcgcaggcg gagggggtat
      901 ccccgttgcg atcgacgagg ccggggcgat acgcggcgtg gaagcggtga tcgataagga
      961 tctttccgcg gccctcctgg ccacccacct gcaggccgac gtccttctca tgctcacaga
     1021 cgtctcggcg gtgtgcaccg actgggggac tcctcaggcc cggccggtcc gtcgtgccac
     1081 gccctcacag ctgcggcggt accggttcga gccgggctcc atggggccga aggtggaagc
     1141 cgcctgccgg ttcgtcgagg ccacgggcgg caccgccgcg atcggtgctt tgggtgacgc
     1201 cgccgcgatc ctggatggcg aggcggggac ggtcgtccgg ctgacgggat gagctgggac
     1261 ggcggcccgc tcgtccggga gcggagggcc ggcgtcaggc ccgcagggtg cgtcgaatcg
     1321 gggcgaccag cgcgttctcg ggatgctcct gaagttcgta tgtcacccga gctaccgggc
     1381 gttcgatgcg gacgagtcgc cgcagatcga tgggcgtggc gcacacgacc gcgtcgcagg
     1441 ggacggcggc gatggtggtg gtcagggtgc cgagctgctc ctcgctgtag cccatcgccg
     1501 gcaggaccgg gcccaactgc gggtgccggc ggtacacggc ggcgagagac gccggggccc
     1561 aggggcgggg atcgacgagc gtggcaccgc gctgctgcgc agcgagcatc ccggcgccgt
     1621 aggccatccc gccgtgggtg agcgtggggc catcttcgac gaccagcact cgccgcccgg
     1681 cgatcagttc ctcgtggtcc accgagatgg cgaggtcggc ctcgacgatg agcgccgagg
     1741 gattggcgcc ttcagccgaa gcccgcacgg cgcgcacggc ctccggcgag gcggtgttga
     1801 ccttgctgac gacgacgagg tcggccatga ggaggttcgt ctcaccggga tgatagcgcc
     1861 gctcgtcccc ggcgcggtgc gggtcgacga tcacgatgtg cagatcggga cggaagaagg
     1921 ggaaatcatt gttgccgcca tcccacacga tcgcatcggc ctcgtcttcg gccgccgcga
     1981 gaacgcggtg cgaatccacg ccggcataga ccaccgtgcc gcgcgccagg tggggctcgt
     2041 attcctcccg ttcttcgatc gtgcagcggt ggcgatcgag gtcctcccac gacgcgaacc
     2101 gctggcacgc ctggtcggcg aggttcccgt aggccatggg gtggcgcacg accacgggac
     2161 gccgcccggc ctcccggatg atggcggcca cgcgacgtgc cgcggggctc ttgccggcgc
     2221 ccgtgcgcac ggcacacacc gcgatgacgg gcttgcgcgc ctcgagcatg gtgcggcggg
     2281 ggccgaggag cacgaaatcg gcgccgccgg ccagcgccac cgatcccagg tgcatgacgt
     2341 gctcatgggg gacatcgctg taggcgaaca cgacctcctg cacgtcgagg cgccggatga
     2401 gctcggggag ttcctcctcc ttgtaaatgg gaatcccgtg tgggtagcgg ggcccggcca
     2461 actgcggggg ataccggcgt ccggcgatgt tggggatctg cgccgccgtg aaggctacca
     2521 ccgcatatcg gggatcgtcg cggtagcaga cgttgaagtt atggaagtcg cggcccgccg
     2581 caccgagaat gagcacgcgc ctggcggtcg ttgccacgcg atgtctccgt ttccgccgcg
     2641 catggggtct cggcgctccg ccgc
//
LOCUS       DASQGH010000136         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45916, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000136 DASQGH010000000
VERSION     DASQGH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45916"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VEW91_02555"
     CDS             <1..423
                     /locus_tag="VEW91_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYM90500.1"
                     /translation="IIEAGRLTGSSMNGQPWRFIVIENRDTLRRLGALARTGPYVAQA
                     PMAVVVAIEKTKFAVSDASRAAQSMILTAWSEGVGSNWVGFLGLTDVKSLLRIPDDLD
                     VLAIIPFGYPVQAHGQGKKQRKLLSEVAHRERFGQPFQ"
     gene            466..813
                     /locus_tag="VEW91_02560"
     CDS             466..813
                     /locus_tag="VEW91_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM90501.1"
                     /translation="MQEAFDLSQVVERRRKSGKSWLKFLRVPALSVGIYSLPAGAEDP
                     QDPHTEDEVYYVVSGRATLRVGSEDRVAEPGSILFVAANVKHRFHSIEEDLTSLVFFA
                     PAAGTERRAGGMG"
     gene            810..>1622
                     /locus_tag="VEW91_02565"
     CDS             810..>1622
                     /locus_tag="VEW91_02565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90502.1"
                     /translation="MMREHGEGADAHTFDVIVVGGGVFGTAIAFFLAERRAGSILLLE
                     SRNIAAGSTGWTSGIVRVHYTNPYEARLASVGSLVFRNWAERVGGECGFLRTGFLRIV
                     GVADREKLLRNVEMLRSLRTDVTFLEPEEVLKLQPYLSVEGVGGAAYEPEGGYASGTD
                     TARALAAAAAGRGVTVRQGVSVRGLRVNADRILGVDTSDGFFAAGRVVVAAGAWSIAL
                     LRQAGVNLPLRTKLVRAGLLLHPAHMSGAGGPYMTVIDDSTGTYFRPDRTNRT"
BASE COUNT          282 a          455 c          576 g          309 t
ORIGIN      
        1 ataattgagg cggggcgact gacgggaagc agcatgaatg gccaaccctg gcgtttcatt
       61 gtcatcgaga atcgcgatac gctacgccgg ttgggtgcat tggcgcgaac cgggccgtat
      121 gtcgcgcaag cccccatggc ggttgtcgtg gcgatcgaga agacgaaatt cgcagtatcc
      181 gatgcgagcc gcgcggctca atcgatgatc ttgacggcgt ggtccgaagg ggtcggctcg
      241 aactgggtgg gctttctggg gttgaccgac gtcaagtcgc ttctgcgcat cccggatgac
      301 ctggacgtgc tggccattat cccgtttggc tatccggtgc aggcccacgg gcaagggaag
      361 aagcagcgaa agttgctctc cgaggtagcc caccgggaga ggttcgggca gccttttcaa
      421 tgacgccccg cgaaatgtgc gccgaggcga cgcggaggtg ccgggatgca ggaggcgttc
      481 gatctttcgc aggtggtcga acggcgtcgg aaatcgggaa agtcgtggct caaatttctg
      541 cgggtgcccg ccctcagcgt gggaatctac tcgcttccgg cgggggcgga ggatccgcag
      601 gatccacaca ccgaggacga agtgtactac gtggtgagcg gccgggcgac gctgcgggtg
      661 gggagtgagg atagggtggc agaacccggg tcgatcctct tcgtcgcggc gaacgtcaag
      721 caccgtttcc acagcatcga ggaggacctc acgagcctgg tgttcttcgc gccggcggcc
      781 ggaacggagc gcagggcagg ggggatggga tgatgaggga acacggggaa ggagcagacg
      841 cgcacacctt cgacgtgatc gttgttggtg ggggcgtctt cgggacggcc attgcattct
      901 ttctggccga gcgtcgcgca gggtccattc tgctcctgga gagcagaaac atcgctgcgg
      961 gatccaccgg atggacgtcc ggcatcgttc gcgtccacta cacgaatccg tacgaggcgc
     1021 gtctggcctc ggtcgggtcg ctggtcttcc gcaactgggc tgagcgcgta ggaggcgagt
     1081 gcggttttct ccggaccgga tttctccgga tcgtcggggt ggccgaccga gagaaactgc
     1141 tgcgcaatgt cgagatgctg cgctccctta ggacagatgt cacgtttctc gaaccggagg
     1201 aggtgctgaa gctccaacca tacctgtccg tggagggagt tgggggcgcc gcctacgagc
     1261 ctgagggagg gtacgccagc ggaacggata ccgcgcgcgc tcttgccgcg gcagccgccg
     1321 gtcgcggcgt gactgtgcgc cagggcgtct ccgtccgggg gcttcgcgtg aatgcggacc
     1381 gcatccttgg ggttgataca tcggatggtt tctttgcggc cggccgtgtg gtcgtggcgg
     1441 ccggcgcgtg gtcgatcgcc cttctcagac aggcaggtgt gaatcttccc cttcgcacaa
     1501 agctcgtccg ggctggtttg cttcttcacc cggcccatat gtctggagca ggcggccctt
     1561 acatgacggt tatcgacgac agcaccggca catattttcg accggaccga accaaccgaa
     1621 cg
//
LOCUS       DASQGH010000137         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6275, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000137 DASQGH010000000
VERSION     DASQGH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6275"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..410
                     /locus_tag="VEW91_02570"
     CDS             12..410
                     /locus_tag="VEW91_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYM90503.1"
                     /translation="MQLIAGCGSNVRFQAGEYLFREGDPSDQFYLIRHGKVSLEISSP
                     AHGRVAIQTLGEGEVVGWSWLVPPYRKQCDARAVELTRATVFDGACIRRKCEADPRLG
                     YEMLKRFAQIIGQRLRATRLQLLDVYGSRP"
     gene            407..1240
                     /locus_tag="VEW91_02575"
     CDS             407..1240
                     /locus_tag="VEW91_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HYM90504.1"
                     /translation="MTGAGGAPAPWRLRRATRETHDTVTLEIEPVGRDGGMAWLPGQF
                     NMLYLFGVGEVPISISGDPAEPRVLVHTVRAVGAVTRGLCRLKRGQVLGVRGPFGSGW
                     PLGPAIGSDVVVVAGGIGLAALRAALYHLLGHRGQYGRIAVLYGARTPDDLLYARGLA
                     QWRGRFDLQIEATVDAAGTGAWAGHVGVVTTLIPRAEFEAAEAIAFVCGPEVMMRFTI
                     ADLRHRGLTADRIYLSMERNMECAIGLCGHCQFGPTFICKDGPVFRYDRVEPLLKIRE
                     V"
     gene            1240..>1706
                     /locus_tag="VEW91_02580"
     CDS             1240..>1706
                     /locus_tag="VEW91_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYM90505.1"
                     /translation="MRSRRRPKLAVWKFASCDGCQLSLLGCEDELLALAGHVEIAYFL
                     EASRERVRGPYDLSLVEGSITTPDDAERIQHVRRVSKSLVTIGACATAGGIQALRNFK
                     DVKEFLRAVYATPAYIATLETSTPIAHHVPVDFELQGCPINKHQLLEVINAFL"
BASE COUNT          232 a          554 c          649 g          271 t
ORIGIN      
        1 cccgggacgc cctgcagctg atcgccgggt gcggcagcaa cgtgcgtttc caggccgggg
       61 agtatctctt ccgcgagggg gatccgtccg atcagtttta tctcatccgc cacgggaagg
      121 tgtcgctgga gatctcttcc cccgcgcacg gccgggtcgc cattcaaacc ctgggggagg
      181 gggaggtggt cggctggtcg tggctggtgc cgccgtaccg gaagcagtgt gacgcgcggg
      241 cggtggagct cacccgggcc accgttttcg acggggcgtg catccggcgg aagtgcgagg
      301 cagacccgcg gctcggctac gagatgctca agcgcttcgc acagatcatc gggcagcgac
      361 tgcgcgcgac gcggttgcag ctcctggatg tctacggatc tcgcccgtga cgggggcggg
      421 gggcgcgccg gcgccgtggc gcctgcggcg ggccacgcgg gagacgcacg acaccgtcac
      481 cctggagatc gagccggtcg ggcgggacgg agggatggcc tggcttccgg ggcagttcaa
      541 catgctgtac ctgttcgggg tgggcgaggt cccgatctcg atcagcggcg accccgccga
      601 gccccgggtg ctcgtccaca cggtgcgggc ggtcggcgcg gtcacgcggg ggctgtgccg
      661 gctcaagcgc gggcaggtcc tcggcgtccg cggcccgttc ggcagcgggt ggccgcttgg
      721 gcccgcgatc gggagcgacg tcgtcgtcgt cgcggggggg atcgggctgg cggcgctgcg
      781 cgcggccctg taccacctgc tcgggcaccg cggccagtac ggccggatcg cggtgctcta
      841 cggcgcccgg acgcccgacg acctgctgta cgcgcggggg ctggcgcaat ggcgcggacg
      901 ctttgacctc cagatcgaag ccacggtgga cgccgccggc accggcgcgt gggccggcca
      961 cgtgggggtg gtgaccacgt tgattccccg cgcggagttc gaggccgcgg aggcgatcgc
     1021 gttcgtgtgc gggccggagg tgatgatgcg cttcaccatc gcggacctcc ggcaccgggg
     1081 cctgaccgcc gaccggatct atctctccat ggagcggaac atggagtgcg cgatcggcct
     1141 gtgcgggcac tgccagttcg ggccgacctt catctgcaag gatgggccgg tgttccgcta
     1201 cgaccgcgtg gagccgctct tgaagatccg ggaggtctga tgcgatcgcg ccgccggccc
     1261 aagctggctg tctggaaatt cgcctcgtgc gacggctgcc agctgagcct gctcggctgc
     1321 gaggacgagc tcctcgcgct ggccgggcac gtggagatcg cctacttcct ggaggcctcg
     1381 cgggagcggg tgcgggggcc gtacgacctc tcgctggtcg agggatcgat cacgacgccg
     1441 gatgacgccg agcggatcca gcacgtgcgc cgggtctcaa agtccctggt gacgatcggc
     1501 gcctgcgcga cggccggggg cattcaggcg ctgcgcaact tcaaggatgt gaaggagttt
     1561 ctgcgggccg tgtacgcgac gccggcctac atcgcgaccc tggagacgtc cacgccgatt
     1621 gcgcaccacg tcccggtgga ctttgagctg cagggctgcc cgatcaacaa gcaccaactc
     1681 ctggaggtga tcaacgcctt cttgaa
//
LOCUS       DASQGH010000138         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3376, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000138 DASQGH010000000
VERSION     DASQGH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3376"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1035
                     /locus_tag="VEW91_02585"
     CDS             91..1035
                     /locus_tag="VEW91_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90506.1"
                     /translation="MTGNLRILLTGACGYLAAQVRPALGEAYDLRLVDVKDTDARGQR
                     VPGVALADLADPDRTKYARLFREIDVVVHFAHKHPGGIPWGAGLPPIDRFDDEFLNIR
                     MAQHVYRSAFDAGVRRVVMASSNHAADWYEHNLIHTRMREIVSASDLPLSDNFYGWAK
                     ASYELLGFTYACGALGRKLQVIQLRIGAPRDVSWRHYAGGLRQQHAGPGGAGLENFKR
                     ELGAYLSERDFCQLLQRAIEAPNIENEYGVPWLVVYGISNNTRAFWSLESARRVLGYD
                     PVDDSEITYADDIRRILTGPEARAPGGRVGARPGARQD"
     gene            complement(1039..2676)
                     /locus_tag="VEW91_02590"
     CDS             complement(1039..2676)
                     /locus_tag="VEW91_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HYM90507.1"
                     /translation="MKAAAAVAEILKREGVEFLIGYPVHPVIEAAATADIRTIIVRQE
                     RTGLHMADAVSRISSGRHIGVFAMQHGPGAENAFGGVAQAYADSVPIVVLPAGYPRRL
                     TNIPPNFNAALNYRHITKWAEQVVLPSEVPAALRRAFTQVRNGRPRPALIEIPTDVLT
                     EEIAEPLVYRPVVAARTAPDPRVVEAAGEALVGAQRPVIYAGQGVHYGRAWGALQELA
                     EWLGAPVTTSLEGKSAFPETHPLALGSGGRSVPQPVHHFLQRSDVIFGIGCSFASTHY
                     GVAMPPGKVIIHATLDPADINKDVPADQVLLGDAGLTLGALLAAVKDRLGAPRREQAS
                     KTAREIAAVRTEWLERWLPKLTSDETPLSPYRVIWDLLHAVDVPNTIITHDAGSPRDQ
                     LSPFWRAIAPLSYIGWGKTTQLGYGLGLAMGAKLEAPEKLCINVWGDAAIGFTGMDFE
                     TAARERIPILSILFNNSCMAIELPVMPVATQKYQSTAISGHYAALAQALGGYGERVTA
                     PREIVPAIKRGVRKTQEGTPALLEFITAQEIEFSLFK"
     gene            complement(2693..3316)
                     /locus_tag="VEW91_02595"
     CDS             complement(2693..3316)
                     /locus_tag="VEW91_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HYM90508.1"
                     /translation="MRVEHLGRRPRVDATAWVAPNAVLSGPVIVGPDARILYGAVLTP
                     EGGAELVVGSECVIMEQAVLRAAGRFPLRLGERVLVGPHAYLSGCRVGARSFVATGAM
                     IFNGAVLGEACVVALGGKVHVDTELADGTWVPMGYIAFGRPGRIHPPEQAPAVHEELN
                     RLGFARYVFGVESAGKPRAEVMDEIMQKYTRALGAHRTDRVDPDAAP"
     gene            complement(3336..4196)
                     /locus_tag="VEW91_02600"
     CDS             complement(3336..4196)
                     /locus_tag="VEW91_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYM90509.1"
                     /translation="MTRAALGLVLVAAFTHATWNLLAKRVGGGPAFVWLFGVLACTIY
                     APLAAAAVLLQRPHIGAVQVGFIAGTSVLHVAYFLMLQNGYRAGDLSLVYPLARGTGP
                     MLSSASAVLLFGERPTPIAMAGTVLIGVGIFLLMGNPGSLRQAGVRRAVGFALLTGAI
                     IAGYTLWDKRAVSALGIPPLLLDWGGNLGRVTILTPVALTRFDEVRQLWRAHRREVLL
                     VALLTPLSYILVLTALVFTPVSYVAPAREISILVGTVMGARLLAEGDAARRLVAAGAM
                     VVGVAALALG"
     gene            complement(4206..>4658)
                     /locus_tag="VEW91_02605"
     CDS             complement(4206..>4658)
                     /locus_tag="VEW91_02605"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYM90510.1"
                     /translation="REQRLGYIATVCPDGTPNLSPKGTTTVWDDDHLLFADICSPGTL
                     TNLAKNPSIEINVVDPMVRKGYRFKGSATILTGGAVFERALAFYRDEQRVADGLVERM
                     QRIVLVKVERALPLISPVYDLGTSETEVAAKWAGYYGALKQGRASVRG"
BASE COUNT          769 a         1604 c         1608 g          677 t
ORIGIN      
        1 ttctccgccc ctattagcgc cgcggcagga ccggcgatag cgcggagccg aagacgaggc
       61 cccgaggaag cgcggacgcg gaggtgcaag ttgacaggaa accttcggat cctcctcacg
      121 ggagcgtgcg gctacctggc ggctcaggtc agacccgcgc tgggggaggc ctacgacctc
      181 cggcttgtcg acgtgaagga caccgatgcg cgggggcagc gggtgccggg ggtcgccctg
      241 gccgacctgg ccgatcccga ccggacgaag tatgcgcggt tgttccggga aatcgacgtt
      301 gtggttcact tcgcgcacaa acacccgggc ggtatcccct ggggggccgg tcttccgccg
      361 atcgatcggt ttgacgatga attcctcaac atcagaatgg cgcagcacgt ctaccggtcc
      421 gccttcgacg ccggcgttcg gcgggtggtc atggcgagct cgaaccatgc ggccgactgg
      481 tacgagcaca acctgatcca cacccggatg cgggagatcg tctcggcctc tgacctgcct
      541 ctgtcggaca acttctacgg ctgggcaaag gccagctacg agctcctggg gttcacctac
      601 gcctgcgggg cgctggggcg caaactccag gtcatccaac tccgaatcgg ggcgcccagg
      661 gacgtctcct ggcggcacta cgcaggagga ctccgccagc agcacgccgg ccccggcggg
      721 gcgggcctcg agaacttcaa gcgcgagctc ggcgcgtacc tcagcgagcg ggatttctgc
      781 cagctcctcc agcgggcgat cgaggccccc aatattgaaa acgagtacgg cgtgccctgg
      841 ctggtcgtgt acggcatcag caacaacacc cgcgccttct ggtcgctcga atccgcgagg
      901 cgggtcctcg gatacgatcc ggtcgatgac tcggagatca cctacgccga tgacatcagg
      961 cgcatcctca caggaccgga ggcgcgcgcg cccggagggc gggtgggcgc caggccaggc
     1021 gcccggcagg actaagtcct acttgaacaa cgagaactcg atctcttggg cggtgatgaa
     1081 ctcgaggagg gccggcgtgc cctcctgcgt cttccgcacc ccgcgcttga tcgccgggac
     1141 gatctcccgg ggggcggtga cccgctcccc gtacccgccg agggcctgcg ccagggccgc
     1201 gtagtgtccc gagatggcgg tgctctggta cttctgggtc gccaccggca tgacgggcag
     1261 ctcgatcgcc atgcaggagt tgttgaatag gatggagagg atcgggatgc gctcccgcgc
     1321 cgcggtctcg aagtccatcc ccgtgaaccc gatcgccgca tcgccccaca cgttgatgca
     1381 gagcttctcg ggggcctcca gcttggcgcc catcgccagc cccaggccat acccgagctg
     1441 ggtggtcttg ccccacccga tgtaggacag cggggcgatg gcccgccaga acggcgacag
     1501 ctggtcgcgc gggctcccgg cgtcgtgcgt gatgatggtg ttgggcacgt cgaccgcgtg
     1561 gagcaggtcc cagatgaccc ggtacggcga cagcggggtc tcgtcagagg tcagtttggg
     1621 gagccaccgc tccagccact ccgtgcggac ggccgcgatt tctctggcgg tcttcgacgc
     1681 ctgctcgcgc cggggggcgc ccagccggtc tttgacggcc gcgagcagcg ccccgagcgt
     1741 caacccggcg tcccccagca ggacctgatc ggccgggacg tccttattga tgtcggcggg
     1801 gtcgagcgtg gcgtggatga tcaccttccc gggcggcatg gccacgccgt agtgggtgct
     1861 ggcgaaactg cagccgatgc cgaagatgac atcgctgcgc tgcaggaagt ggtggacggg
     1921 ttggggcacg gagcggccgc ccgagccgag cgcgagcgga tgggtttcgg gaaaggcgct
     1981 cttgccctcg aggctcgtgg tcaccggcgc gccgagccac tcggcgagct cctgcagggc
     2041 cccccaggcg cgtccgtaat ggaccccctg gccggcgtag atgaccgggc gctgcgcgcc
     2101 cacgagcgcc tccccggccg cctcgaccac ccgcgggtcc ggcgcggtgc gggcggcaac
     2161 gaccgggcgg tagaccagcg gctcggcgat ctcctcggtc aggacgtccg tcgggatctc
     2221 gatgagcgcg gggcgggggc gcccgttccg gacctgggtg aacgcgcggc gcagcgccgc
     2281 gggcacttcg gaggggagca ccacctgttc cgcccacttg gtgatatggc ggtagttcag
     2341 ggcggcgttg aagttcggcg ggatattcgt gaggcggcgg gggtatcccg ccggcagcac
     2401 cacgatcggc accgagtcag catacgcctg ggcgacgccg ccgaaggcgt tctccgcgcc
     2461 cgggccgtgc tgcatggcga agaccccgat gtggcggccg gatgagatcc ggctgaccgc
     2521 gtcggccatg tgaagcccgg ttcgctcctg acggacgatg atggtgcgaa tgtccgccgt
     2581 ggccgccgcc tcgatcacgg ggtggacagg atagccaatc aggaactcca cgccctcgcg
     2641 cttcaggatc tccgccacgg ccgcggccgc cttcacaggt catcctcccc cctcatggtg
     2701 cggcatcggg atcaaccctg tcggtccggt gggcgcccaa cgcgcgagtg tacttctgca
     2761 tgatctcatc catgacctca gcccggggct tccccgccga ctcgaccccg aacacatacc
     2821 tcgcaaagcc gagccggttc agctcctcgt gcaccgcggg ggcctgttcg gggggatgga
     2881 tccggcccgg ccggccgaag gcaatgtagc ccatcgggac ccaagtgccg tcggccagct
     2941 cggtgtccac atgcacctta ccgcccaacg ccaccacgca ggcctcgccc agcaccgccc
     3001 cattgaagat catcgcgccg gtggccacga agctgcgcgc tcccacgcgg catcccgaga
     3061 gataggcatg gggtcccacg agcacccgct cgccgagccg cagggggaat cggccggcgg
     3121 cacgcaggac ggcctgctcc atgatcacgc actccgaccc caccacgagt tcagccccgc
     3181 cctcgggggt gagcacggca ccgtagagaa tgcgggcgtc gggcccgacg attacgggtc
     3241 ccgaaaggac ggcgtttgga gcgacccacg cggttgcatc cacccgcggc cgacggccca
     3301 ggtgttccac tcgcatcccg aatcacctcc cggccctacc ccagcgccag cgcggccacg
     3361 cccaccacca tggctccggc cgccaccagg cggcgcgcgg cgtctccttc ggcgagcagg
     3421 cgggccccca tgaccgtgcc taccaggata ctaatctccc gagccggggc gacgtagctg
     3481 accggggtga acaccagggc ggtcaggacc aggatgtagg acagcggggt cagcagggcc
     3541 accaggagga cctcgcgccg gtgcgcacgc cacagttggc ggacttcatc gaaccgcgta
     3601 agcgccaccg gtgtgaggat ggtcacccgg cccagattgc cgccccagtc gaggagcagc
     3661 ggggggatcc cgagggcgct gaccgcgcgc ttgtcccaga gggtgtaccc ggcgatgatc
     3721 gcgccggtga gcagagcgaa gccaacggcc cggcgcaccc cggcctgtcg caggctcccc
     3781 gggttgccca tcaggaggaa gatgcccacc ccgatcagca ccgtgccggc catggcgatg
     3841 ggggtggggc gctcgccgaa cagcaggacc gccgaggcgc tagacagcat cggacccgtt
     3901 ccgcgggcca ggggatagac cagggaaaga tcgccggccc ggtagccgtt ttgaagcatg
     3961 agaaagtacg ccacgtgaag caccgaggtg ccggcgatga agcccacctg gacggcgccg
     4021 atgtgggggc gctggaggag gacggccgcc gcggccagcg gagcgtaaat ggtacaggcg
     4081 aggacgccga acaaccacac aaacgccggg ccgccaccca cccgcttcgc cagcaagttc
     4141 caggtggcgt gggtgaacgc cgcaacgagc acgaggccga gggcggcccg ggtcatgtcg
     4201 tgaggctagc cccgcaccga tgcccgcccc tgcttcagcg ccccgtaata gcctgcccac
     4261 ttggccgcca cctccgtctc ggaggtgccc aggtcgtaga ccggcgagat cagcggcagg
     4321 gcccgctcga ccttgaccag cacgatccgc tgcatccgct cgacgagccc atcggcgacg
     4381 cgctgctcgt cccggtagaa tgcgagggcc cgctcgaaca cggcgcctcc ggtgagaatc
     4441 gtggccgatc ccttgaagcg gtatcccttt cgcaccatgg ggtccacgac gttgatctca
     4501 atcgacggat tcttcgcaag gtttgtgagc gttcccggcg agcagatgtc ggcgaagagg
     4561 agatgatcat cgtcccagac ggtggtcgtc cctttcggag acaggttcgg cgtcccgtcg
     4621 gggcagaccg tagcgatata gccgaggcgc tgctcacg
//
LOCUS       DASQGH010000139         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54316, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000139 DASQGH010000000
VERSION     DASQGH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54316"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VEW91_02610"
     CDS             <1..824
                     /locus_tag="VEW91_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HYM90511.1"
                     /translation="NRVTRPILVRAAGMLVGMIEAAPPSGTAGDAARAPAAVAVTALG
                     NTHAAVTRVMADLGARGYEVVPFHASGAGGSAMEWLVRQGEFAAVIDLTPHEFLGEVL
                     GDDIYTPVHPGRLTAAGAAGIPQVVAPGGLDYFVFGAEETVPPRYRGRPTHHHNPYNT
                     NVRASADELHRVGKALAERLNNARGPVAFLVPLRGWSHIGREGGPLWDPQANEALREV
                     MRRTLRTGLRYIEVDAAINDPSFADEVAAVACEFLGPGNGAARPRERAEGGVQCS"
     gene            815..1918
                     /locus_tag="VEW91_02615"
     CDS             815..1918
                     /locus_tag="VEW91_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYM90512.1"
                     /translation="MLVREAATHKIPEVMQAWALFGPGDLRRITKPVPHPGEAEVLVK
                     IEAVAICATDIEILHHGLPAMVEGRPPFNGQHVIGHEYAGTVAALGPAVDEFSLGDRV
                     VVEVHAGCGRCERCRHGMYTSCLNYSNRAKGHRANGFTTDGGFAEYAVNHVNTLFRLP
                     DHLAFEEATLVVTAGTAIYGLDVLGGLVAGETLLVTGPGPIGLMAVACGKALGAHVIL
                     TGTRENRLSLGRRLGADETVNVKTQNAVEAIKALTHGVGVDLVIECSGAPTAINDALY
                     VTKRGGRICLAAFSRDPVPFDAAHMVRNNIYLYGIRGEGHGAVKRGLALMAQGKISGK
                     PFITHRFAMAELPRALETAEKRLGDAIKVVVTP"
     gene            1925..2746
                     /locus_tag="VEW91_02620"
     CDS             1925..2746
                     /locus_tag="VEW91_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90513.1"
                     /translation="MAGDARYLRGPVARWLRLNGFVVLAYLLAAGAWQAASAVFHVPE
                     FLLPSPTAILEKAAHVPSQLVHHSAITLLEVLEGFLLGAVVSVPLGILVVYSRPLERL
                     IYPFLVAFQAIPKVALAPILVVWFGFGPASKVTLALVTAMFPIVVNTVIGMRQTPPEL
                     VYMMRSLRASEAQIFLKIRLYAAAPYVFAGFKIGITLAVVGAIVGEFIASNGGLGYLL
                     LAANNNIDAPLLFAVVGVLSALSIALYYAVEAVERFLLPRPLRSRSVKLEQRLRP"
     gene            2770..3537
                     /locus_tag="VEW91_02625"
     CDS             2770..3537
                     /locus_tag="VEW91_02625"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90514.1"
                     /translation="MAAVEVDQISVAYALDSGDRLQALDRLSLQIPAGRFCSIVGPSG
                     CGKSTLLMSIGGLYRPTAGRVLIDGQAVVGPWHGVGMVFQRDVLLEWRTALENVLLPV
                     EVKHLPIARYRDRARALLDLVGLGAFADVYPDQLSGGMRQRVASCRALIHDPPLLLMD
                     EPFAAVDALTREKLNADLLRLITESPKTVVFVTHNIEEAVMLADQVAVMGPRPGRVLR
                     TFPVEIPRPRDPGTRNDPRFHGLVDKIRGVFRTMGLL"
     gene            3623..>3939
                     /locus_tag="VEW91_02630"
     CDS             3623..>3939
                     /locus_tag="VEW91_02630"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90515.1"
                     /translation="MRGASRRSVLRGGLALLAARLIAPRALGAAAATGPAKQATLRFN
                     WTIKGEFTPFFVAREKGYYAEAGVTLELSEGKSGTQAVEVVGAGRDTFGYVPSIQVIE
                     AIN"
BASE COUNT          576 a         1323 c         1426 g          614 t
ORIGIN      
        1 cgaaccgtgt gacccgaccc atcctggtcc gcgccgccgg gatgctggtg gggatgatcg
       61 aggcggcccc tccgtccggc acggccgggg acgccgcgcg ggccccggcc gcggtcgccg
      121 tgaccgcgct cggcaacacg cacgccgccg tcacccgggt gatggcggat ctcggggcgc
      181 gaggatacga ggtggtgccg ttccatgcct ccggggccgg cgggtcggcc atggaatggc
      241 tcgtgcggca gggggagttc gcggcggtga tcgatctgac gccccacgag ttcctgggcg
      301 aagtcctcgg cgacgacatc tacaccccgg tccaccccgg acgcctcacc gcggccggcg
      361 cagcggggat cccgcaggtc gtcgctccgg gggggctcga ctactttgtg tttggggcgg
      421 aggagacagt ccccccgcgc tatcggggcc ggccgacgca tcatcacaac ccgtacaaca
      481 cgaatgtccg ggcgagcgcc gacgagcttc accgtgtcgg gaaggcgctg gcggagcggc
      541 tgaacaacgc gcgcggcccg gtggccttcc tcgtcccgct gcggggatgg tcgcacatcg
      601 gccgcgaagg cggccctctc tgggaccccc aggccaacga ggcgctgcgg gaggtgatgc
      661 gccgcacgct ccgcacaggt ctccggtata tcgaggtcga cgccgcgatc aacgatccgt
      721 cctttgccga cgaggtggcg gcggtggcct gcgagttcct gggtcccggg aacggcgcgg
      781 cgcggccgcg tgagcgggcg gaaggaggcg tgcaatgctc gtgagggaag cggcgacgca
      841 caagatcccc gaggtgatgc aggcgtgggc gctgttcggc ccgggcgatc tccggcggat
      901 cacgaagccg gtgccccatc cgggcgaggc ggaggtcctg gtcaagatcg aggcggtggc
      961 gatctgcgcc accgacatcg agatcctcca ccacgggctg ccggcgatgg tggaggggcg
     1021 gccgcccttc aacggccagc acgtcatcgg gcacgagtac gccggcaccg tcgccgcgct
     1081 cgggcccgcg gtcgacgagt tcagcctggg cgaccgcgtc gtcgtggaag tgcatgccgg
     1141 gtgcggccgt tgtgagcggt gccggcacgg catgtatacc tcctgtctca actactccaa
     1201 ccgggccaag ggacaccggg ccaacgggtt cacgacggac gggggcttcg ccgagtatgc
     1261 cgtgaaccac gtcaacacgc tgtttcggct gcccgatcac ctcgcgttcg aggaagccac
     1321 gctcgtcgtg accgcgggca cggcgatcta cggtctcgac gtcctcgggg gcctggtcgc
     1381 gggcgagacc ctcctggtga cgggccccgg cccgatcggg ctgatggcgg tggcctgcgg
     1441 caaggcgctg ggcgcccacg tgattctgac cgggacccgc gagaaccggc tgagcctggg
     1501 gaggcggctc ggggccgacg agacggtgaa tgtcaagacg cagaacgcgg tggaggcgat
     1561 caaggcgctg acgcacggcg tcggcgtgga cctcgtcatc gagtgctcgg gggcccccac
     1621 cgccatcaac gacgcgctgt acgtgacgaa gcgaggcggc cggatctgcc tcgcggcctt
     1681 cagccgggac cccgtgccgt tcgatgccgc gcacatggtg cgcaacaaca tctacctgta
     1741 cggcatccgc ggggaaggcc acggggcggt gaagcgcggc ctcgccctca tggcgcaggg
     1801 aaagatctcg ggaaagccgt tcatcacgca ccggttcgcg atggcagagc tgccccgcgc
     1861 gctcgagacc gcggagaagc gcctgggcga cgccatcaag gtcgtcgtca ccccgtagaa
     1921 gcccatggcg ggcgatgcgc ggtaccttcg aggtccggtc gcccgatggc tgcggctcaa
     1981 cggcttcgtc gtgctcgcct acctgctcgc cgccggtgcg tggcaggcgg ccagcgccgt
     2041 cttccacgtt ccggagtttc tcctgccctc tcccacggcg atcctcgaga aggctgcgca
     2101 cgtcccgtcc cagctggtcc accattcggc gatcacgctg ctggaggtcc tggagggctt
     2161 cctgctgggc gcggtggtca gcgtgccgct tgggatcctg gtggtctact cccgcccgct
     2221 cgagcgcctc atctatccgt ttctggtggc gtttcaggcg attccgaagg tcgcgctggc
     2281 tccgatcctg gtggtctggt tcgggttcgg cccggcctcg aaagtgaccc tcgcgctggt
     2341 gacggcgatg ttcccgatcg tcgtcaacac cgtgatcggc atgaggcaga cgccgccgga
     2401 gctcgtgtac atgatgcgct cgctcagggc atcggaagcc cagatcttcc tcaagatcag
     2461 gctctacgcg gcggccccct acgtgtttgc cgggttcaag atcgggatca cgctcgcggt
     2521 ggtgggcgcg atcgtcgggg agttcatcgc gtcgaacggc ggcctcgggt acctgctgct
     2581 ggccgccaac aacaacatcg acgccccgct gctcttcgcg gtggtggggg tgctgtccgc
     2641 gctcagcatc gcgctctact acgccgtgga agccgtcgaa cgcttcctcc tgccccggcc
     2701 cctgcggagc aggagcgtga agctcgagca gaggctccgt ccgtgaggag cggagtgggg
     2761 acgacccagc tggccgccgt ggaggtggac cagatctcgg tcgcgtatgc gctcgacagc
     2821 ggcgaccgcc ttcaagcgct cgaccgcctg agcctacaga ttccggccgg ccgcttctgc
     2881 agcatcgtcg gaccgagcgg ctgcgggaag agcaccctct tgatgtcgat cgggggcctc
     2941 taccgtccca ccgcggggcg cgtcctgatc gacgggcagg cggtcgtcgg cccgtggcac
     3001 ggggtcggca tggtgtttca acgggacgtg ctgctcgaat ggaggacggc cctcgagaac
     3061 gtgctcctgc cggtcgaggt caagcacctg ccgatcgccc gctaccgcga ccgggcgcgc
     3121 gccttgcttg accttgtggg gctcggggcg tttgccgacg tctaccccga ccagctgtcc
     3181 ggcgggatgc gtcagcgcgt ggcgagctgc cgggcgctga tccatgatcc gccacttttg
     3241 ctcatggacg agcccttcgc cgcggtggat gccctgacgc gcgagaagct gaacgcggac
     3301 ctgctccgcc tgatcacgga gtcacccaag accgtggtgt tcgtcacgca caacatcgag
     3361 gaggcggtga tgctggccga ccaggtcgcg gtgatgggcc cccggccggg gagggtactg
     3421 cgcacctttc cggtcgagat cccacgcccc cgggacccgg ggacacggaa cgacccccgg
     3481 tttcacgggc tggtcgacaa gatccgcggg gtctttcgca ccatgggcct gctatgagcg
     3541 atcgcggcgc gcgccagaag cgttgtcccg aggggaggga tccgaggtga cacacacgat
     3601 ggcagggcca cggcggaaga cgatgagagg cgcgtcccgc cggtccgtcc tccgcggcgg
     3661 gctggcgctc ctcgccgccc ggctcatcgc cccgcgggcg ctgggggcgg cggcggccac
     3721 gggtcctgcg aaacaggcca ccctgcggtt caactggacg atcaaggggg agttcacccc
     3781 cttcttcgtc gccagagaga aggggtacta cgcggaggcc ggcgtgacgc tcgagctgtc
     3841 ggagggcaag agcgggaccc aggcggtcga agtcgtcggg gcggggcgcg acacgttcgg
     3901 atacgtccct tcgatccagg tgatcgaggc gatcaacag
//
LOCUS       DASQGH010000140         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34510, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000140 DASQGH010000000
VERSION     DASQGH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34510"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VEW91_02635"
     CDS             <1..1120
                     /locus_tag="VEW91_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYM90516.1"
                     /translation="DVYINPTVLNIEGKRMSKTMGTGMDPLLLVDRYGADSLRFALIN
                     RCTGEQDLRFSEKMVEDTRNFANKIWNAARFVRMNLDDAPAPAVPPARAALGLPERWI
                     LSRFGRCALAVTQSLERFEFREVCQHLYDFVWSEFCDWYVEMAKLDLREPIAPGHPAA
                     TRAVLSWTLAETMKLLHPIMPSLTEEIWQALPHEGRTIMRARWPEDAAGWIDPDAEAR
                     MTQVVDVVRAIRGIRADLGLPPGELVTVALFAPDEPRAVLESAARYLAPLARVRDFGV
                     HRESAPRPSGALATLCGAVEIALALDSKEAAQAARTRLQKQLATVARDLASLDGRLQS
                     RAFIEKAPAEVVAADRHRRDELSARRATLERYLGGLGR"
     gene            1110..>1901
                     /locus_tag="VEW91_02640"
     CDS             1110..>1901
                     /locus_tag="VEW91_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYM90517.1"
                     /translation="MDASRAPEQAAPARWVSLLLLGAGIAILVAFAATTNYRQIGDAL
                     RGIRGSLLALAAGAVGSQILLKAVRWRFMIERLTGTAISWRFGAVSILSGVAAGSITP
                     GRSFELAKAVMLKGSYDIPLGVSTSAMIVERMLDIVFLVVTFLGCAAFVPTRMVLASR
                     LLALLIAALIGATALVVVAPAGVQAWIGAGVRRLPVPPALRDRGLRLLETFFSSLLLL
                     RGQRTLWPLLSMTFVVTALDVIRVFTVFRAMGVPLVLAVVAFAYLG"
BASE COUNT          257 a          642 c          679 g          323 t
ORIGIN      
        1 ggacgtctac atcaatccca ccgtcctcaa catcgaggga aagcggatga gcaagacgat
       61 gggcaccggg atggacccgc tcctcctggt cgaccgctac ggggccgact cgttgcggtt
      121 tgccctgatc aaccggtgca ccggtgagca ggacctccgg ttctcggaaa agatggtcga
      181 ggacacgcga aacttcgcca acaagatctg gaacgccgcc cgcttcgtcc ggatgaacct
      241 cgacgatgcc ccggccccgg ccgtgccgcc cgcccgggcg gccctgggcc ttcccgagcg
      301 gtggatcctc agccggttcg ggcggtgcgc cctggcggtc acgcagtccc tggagcggtt
      361 cgagtttcgc gaggtctgcc agcacctgta cgacttcgtc tggagcgagt tctgtgactg
      421 gtacgtggag atggcgaagc tggatcttcg ggagccgatc gcccccgggc accccgcggc
      481 tacccgcgcg gtgctgagct ggaccctggc ggagacgatg aagctgctcc acccgatcat
      541 gccgtccttg acggaagaga tctggcaggc gctcccgcac gagggacgca cgatcatgcg
      601 cgcgcgctgg ccggaagacg cggcggggtg gatcgaccct gacgcggagg cccggatgac
      661 gcaggtcgtc gatgttgttc gcgcgatccg gggcatccgc gccgacctcg gcctcccacc
      721 gggcgaactg gtcacggtgg ccctgttcgc acccgacgaa ccccgggcgg tgctggaatc
      781 ggccgcccgc tatctcgcgc cgctggcccg ggtccgcgac tttggggtcc accgggagag
      841 cgcgccgcgg ccgagcgggg cactcgcgac cctgtgtggg gccgtggaga tcgccctggc
      901 gctggactcg aaggaggccg cccaggcggc ccggacgcgg ctccaaaagc aactggccac
      961 ggtcgcgcga gatctcgcga gcctggatgg cagactccag agcagagcgt tcatcgagaa
     1021 agcgcccgcc gaagtcgtgg cggcggaccg gcaccggaga gatgagctat cggccaggcg
     1081 cgcaacgctg gagcgctacc tgggcggcct tggacgctag ccgcgcgccc gagcaagccg
     1141 cccccgcgcg atgggtcagc ctcctcctgc tgggggccgg gatcgcgatc ctggtggcgt
     1201 tcgccgcgac caccaactat cgccagatcg gggacgccct gcgcgggatt cggggatcgc
     1261 ttctcgcact cgcggccggg gcggtgggga gccagattct gctcaaagcc gtccggtggc
     1321 ggttcatgat cgagcggctg accggcacgg cgatctcttg gcggttcggc gcggtctcga
     1381 tcttgtccgg ggtcgccgcc ggcagcatca ccccgggccg gagtttcgaa ctggccaagg
     1441 ccgtcatgct gaagggctcg tacgacatcc cgctgggggt cagcacgtcg gcgatgatcg
     1501 ttgagcggat gctcgatatc gtcttcctcg tggtgacgtt tctcggctgc gcggcctttg
     1561 tcccgacccg gatggtcctg gcgagccgcc tgctggcgct gttgatcgcg gcgctgatcg
     1621 gggccaccgc cctcgtggtc gttgccccgg cgggggtgca ggcctggatc ggggccggcg
     1681 tgcggcgtct cccggttccc ccggccctcc gcgatcgggg tctccggctc ctggagacgt
     1741 ttttctcgag cctcctgctc ctccggggcc agcggacgct ctggccgttg ctcagcatga
     1801 cgtttgtggt gaccgcgctg gatgtcatcc gggtcttcac cgtcttccgc gccatggggg
     1861 ttcccctcgt gctcgcggtg gtggcgttcg cgtatctggg g
//
LOCUS       DASQGH010000141         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40243, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000141 DASQGH010000000
VERSION     DASQGH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40243"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            662..>2521
                     /locus_tag="VEW91_02645"
     CDS             662..>2521
                     /locus_tag="VEW91_02645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYM90518.1"
                     /translation="MEPKMPFGSWKSPITSDLIVSRTIAFEGIALDGEEIYWTETRPA
                     EAGRYVIVRRSPDGAVVDVTPPSLNARTLVHEYGGGAFTVGDGVVYFSNFADQRLYRQ
                     AAAAPPTPLTPPGKWRYADIIIDHRRNRLIAVREDHSDPTREAVNTLTGIDLGAPAEG
                     RALVSGNDFYASPRLSPDGARLAWLTWRHPNMPWDGTELWVAEIGQDGRPVRPERVAG
                     GQTESIFQPEWSPGGVLYFVSDRTGWWNLYRWLAGRIEPLREMEAEFGAPQWRFGMST
                     YAFESEARIVCTYKARGRWHLAQLDTESRALRELDTPHSAISSVRASPGCVVFEGGGP
                     ESFSAIVRLDIRTGRCETLKQSSAVTVDPGYLSVPDAIEFPTEHGQTAHASFYAPRNR
                     DYTAPAGEKPPLLVRSHGGPTSAASATLNLGIQYWTSRGFAVLDVNYGGSSGYGRAYR
                     ERLNGEWGVVDVDDCVNGARHLVQRGLVDARRLAIRGSSAGGYTTLCALTFRSFFAAG
                     ASYYGIGDLEIFVRDTHKFESRYLDRLIGSFPERRDLYRARSPIHFIDRIACPMILFQ
                     GLEDKIVPPNQAELMVAALRARGLPVAYVAFEGEQHGFLKAENIKRALEAELYF"
BASE COUNT          419 a          801 c          863 g          438 t
ORIGIN      
        1 gtgaccccga taacgccgcc gcctaggacc agtatcctca tacgtcacgt tcgccccttc
       61 ccgtctgagc atggaccaca atggatgctg gcatcgagtg cgataccctg tcggcgtccg
      121 cccccgtagg ggagtcgctg gggtggaaac gccgttgagg agacccaatc gtaaacgggt
      181 ccggttcgtg ccgctgacca cggtcgcggg ccggatgccg cgccgcggct gggacagatc
      241 gaggttcttc gggactactt cagctccacc gtttccatgt tctcgttccc attcggctgc
      301 gcgagcgtag atgtcaccat ggcagttctt gaaagccctc gcaccctcat ggttgcgccc
      361 tcctaggcat gaccggcacg tgtggaccga gaccacgcgc gtgttctgca ccgctgtcgc
      421 ggggacctcc tgcgcgagcc ctgcgcactc cgctcccgag gcgccaccgg aagacccgcg
      481 tttcggcgaa cgggaggagg agaagcgcgt ggcacggccg actcatagat gagaaatcgg
      541 ctgcaggagc ggccatcccg ggttttgaag ttcttcgggg gcgtggaacg gacttttctt
      601 ggggtcccgg ggcatggtct gggctcaagt ccgggcaggg aacccacaaa gagaaggtgt
      661 tatggaaccc aaaatgccgt ttggatcctg gaagtccccg atcacctccg acctgattgt
      721 ctcgcgcact atcgcgtttg agggcatcgc cctcgacggc gaggagatct actggaccga
      781 gacgcgtccc gcggaagccg gccgctatgt catcgtgcgg cgcagcccgg acggcgcggt
      841 ggtcgatgtg accccgccgt cgctcaacgc ccggaccctc gttcatgaat acggcggtgg
      901 cgcgttcacg gtcggcgacg gggtcgtgta cttctccaac ttcgccgacc aacggctgta
      961 ccggcaagcc gccgcggcgc ctcccacgcc gctgacgcct ccgggaaagt ggcggtacgc
     1021 cgatatcatc atcgatcatc ggcgtaatcg actgatcgcg gtaagggagg accacagcga
     1081 tcccacgcgc gaggccgtca acacgctcac cggcatcgat cttggggccc cggccgaggg
     1141 ccgcgcgctc gtctcgggca acgacttcta cgcgagcccg cgcctgagcc cggacggggc
     1201 tcgactggcg tggttgacgt ggcgccatcc gaacatgccc tgggacggga cagaattgtg
     1261 ggtggcggag atcggacagg atggcaggcc agtgcggcct gaacgcgtcg cgggcggcca
     1321 aaccgaatcg atcttccagc cggagtggtc cccggggggc gttttgtact tcgtctcgga
     1381 tcgcacgggt tggtggaacc tgtaccggtg gctagccggg cggatcgagc cgctccgtga
     1441 aatggaggcg gagttcggcg cgccgcagtg gcggttcggg atgtcgacgt acgccttcga
     1501 gtcggaggcg cgtatcgtct gcacgtacaa agcgcgcggc cgatggcatc tcgcccagct
     1561 cgacaccgag agccgggcgc tgcgggagtt ggacacgccg cactctgcga tctcgtcggt
     1621 gcgggccagt cccgggtgcg tggtgttcga gggcggcggg cccgagtcgt tttcggcgat
     1681 cgtgcggctc gacatccgca ccggccggtg cgagactctg aagcagtcga gcgcggtgac
     1741 ggtggacccg ggctatctgt cggtcccgga cgcgatcgag ttcccaacag agcacggcca
     1801 gacggcgcac gcgtctttct acgcgccgcg caaccgcgac tacaccgcgc ccgccgggga
     1861 gaagcctccg ctccttgtga ggagtcacgg cggtcccacg agcgccgcgt ccgcgaccct
     1921 caacctgggg atccagtact ggacgagccg cggcttcgcg gtgctggatg tgaattacgg
     1981 gggtagttcc ggatacgggc gtgcataccg cgagcggctc aacggcgagt ggggcgtcgt
     2041 ggatgtggac gactgcgtga acggtgcgcg gcatctggtg caacgcgggc tcgtcgatgc
     2101 acgccgcctg gcgatccggg gcagcagcgc gggaggctac acgacgctgt gcgcgctgac
     2161 gtttcggtcc ttctttgcgg ccggcgccag ctactacggg attggcgacc tggagatctt
     2221 cgtgcgggac acgcacaaat tcgagtcccg gtacttggac aggctgatcg gatccttccc
     2281 ggaacgacgc gacctttacc gggcgcgctc gccgatccat ttcatcgatc ggatcgcctg
     2341 cccaatgatc ctcttccaag ggctggagga caagatcgtg ccacccaatc aggcggaact
     2401 catggtcgcg gccctgcggg ccagaggcct cccggtcgct tacgtcgcct tcgagggaga
     2461 gcaacacgga ttcctgaagg cggagaatat caagcgggcg ctggaggcgg aactatactt
     2521 c
//
LOCUS       DASQGH010000142         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14707, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000142 DASQGH010000000
VERSION     DASQGH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14707"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VEW91_02650"
     CDS             complement(<1..1118)
                     /locus_tag="VEW91_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYM90519.1"
                     /translation="MGRQEVVRMAVMSGSEAMVEALRNEGVTIVMGVVGSAFMDALDI
                     FPQAGIRFLPVRHEQSAAHMADGYARVTGRPGVCTGQNGPGVTNMVTGIAAAYLAHSP
                     VILLSPAATSASVGTDGFQETEQMKIFAGITKYQVRVDRADRFPEFFRNAYRIALAEL
                     GPVQVDFPRDYLYHEVDVEIIPPDRYRVAARGAGDPGALQAAAALLAKAEHPVLLAGL
                     GTVYAGAQGEVAELAEFLTAPVVTSYLHNDAFPARHPLAAGPIGYGGSKAAMQVLSKA
                     DVVLALGSRLSIFGTLAQYGFDYWPKGAKLIQVDVNPRQIGRVKPFDVGILGDAKVAA
                     AEILRRVRVLLPDRKPHSARLDAVRQAKQAWAEELQKLS"
     gene            complement(1182..1964)
                     /locus_tag="VEW91_02655"
     CDS             complement(1182..1964)
                     /locus_tag="VEW91_02655"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYM90520.1"
                     /translation="MDVRGKAAIVTGSGSAGGIGRSTAIHLAQHGAAGVVVNYSNPRS
                     EGKAAAVAAEVERAGARACLHRADVSDDRQCRAMVDAAVHAFGRLDILVNNAATTVRV
                     RYEDLEALTDEIWDRILTVNLKGTFYCIRAAAPHLAASGEGAIINVASVAGVRAVGAS
                     SIPYAASKAAVINMTMLLARALAPRVRVNCVTPGFVAGQWMEESLGPDRYEQAAERTR
                     QRIPMGRVATPDDVAHAILSLITSDFITGHNLICDGGYLIRD"
     gene            complement(1984..>3125)
                     /locus_tag="VEW91_02660"
     CDS             complement(1984..>3125)
                     /locus_tag="VEW91_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90521.1"
                     /translation="SVTAVDPTTVQITLERPYSPLLYVLTDRAGMMVSPTAAQKEGLN
                     YALHPVGTGPFSFVEKIPADHITLQRNPEYWEKGVPYADRIVFRTITDDNARVANVKS
                     GDADIINVVPLPQARELAREAAQPGARFKLIERGAIAWTAIALNVTRPPFDNKLLRQA
                     FAATIDRDALANVVLQGAAYPAHAFFPNGTPAYEPGWKLPPRSVALAKEKLQAAGHAG
                     GFTFTLLNQPGQQRQAVAQAIQAMAGDAGIEMKIQIVDEGTIIDAISHLREEGALIEW
                     SGRPDPDFDIYPFTTASGIGSFNYAGYNNPRMETLLDAARVLNDMSQRRRAYREVTQI
                     LADDVPYVWLYFPKEYKLISTRLRGFVQVPDGMIRLRKAWLSPVR"
BASE COUNT          472 a         1085 c         1053 g          515 t
ORIGIN      
        1 gacagtttct ggagttcctc ggcccacgcc tgcttggcct gacggacggc gtcgagccgc
       61 gccgaatgcg gcttccggtc cgggagaagc acccggacac gacggagaat ctccgcggcg
      121 gccaccttgg cgtctccgag aatccccacg tcgaacggct tcacccggcc gatctgccgc
      181 gggttgacgt cgacctgaat caacttggcg cccttgggcc agtagtcgaa cccgtactgc
      241 gccagggtgc cgaagatgct gagccgcgac cccaatgcca gcaccacgtc ggccttggac
      301 agcacctgca tcgcggcttt ggagcccccg tagccgatcg gccctgcggc aagggggtgc
      361 cgggcgggga aggcgtcgtt gtggagatag gatgtgacca cgggcgcggt aagaaactcg
      421 gccagctccg ccacttcccc ctgcgcgccc gcgtagaccg tcccgagacc ggcgaggagc
      481 acggggtgct ccgcctttgc caggagcgcg gcggccgcct gcagggcgcc cggatcgcca
      541 gccccgcgcg cggccacgcg gtagcggtcc gggggaatga tctccacgtc cacctcgtga
      601 tagaggtagt cccgcgggaa atccacctgc accgggccga gctccgccag cgcgatccgg
      661 tacgcgtttc ggaagaactc cgggaagcgg tcggcgcggt cgacccggac ctggtacttg
      721 gtgatcccgg cgaagatctt catctgctcg gtttcctgga acccatcggt ccccacggat
      781 gccgacgtgg ccgcgggcga gagcagaatg accggcgaat gcgccaggta cgctgccgcg
      841 atcccggtca ccatgttggt cacgccgggc ccgttctggc ccgtgcacac ccccggccgg
      901 cccgtcacgc gggcgtagcc gtcggccatg tgggccgcgc tctgttcatg gcggaccggg
      961 agaaagcgga tgcccgcctg ggggaagata tcgagggcgt ccatgaacgc ggagcccacc
     1021 acccccatca cgatcgtgac gccttcgttc cgaagcgcct ctaccatcgc ctcgctgccg
     1081 ctcatcacgg ccatgcgcac cacctcctgc ctccccaccg ggagctccct cgctgctgtt
     1141 tcttcggtct cgcccgagaa agtcctcggt cacgcgcgcg cctagtctcg gatcaggtag
     1201 cccccgtcgc agatcaggtt gtgccccgtg atgaaatccg aggtgatcag ggagaggatc
     1261 gcgtgggcca catcgtccgg ggtcgcgacg cgccccatcg ggatccgctg gcgtgtccgc
     1321 tcggcggcct gctcgtagcg gtcgggcccc aggctctcct ccatccactg gccggccacg
     1381 aacccggggg tcacgcagtt cacccggacg cgcggcgcga gcgcgcgcgc cagcagcatc
     1441 gtcatgttga tcacggccgc tttcgatgcg gcgtacggga tcgaggaggc cccgaccgcg
     1501 cggaccccgg caaccgacgc cacattgatg atcgccccct cgccggacgc ggcgaggtgg
     1561 ggcgcggcgg cgcggatgca gtagaacgtc cctttcaggt tcacggtgag gatccgatcc
     1621 cagatctcgt ccgtgagggc ttccagatcc tcgtacctca cccggaccgt ggtggccgcg
     1681 ttgttcacca gaatgtccag ccggccgaac gcgtgcacgg cggcatccac catggcacgg
     1741 cactgccggt cgtcggatac gtcggcacgg tggaggcagg cccgcgcccc ggcccgttcg
     1801 acctcggcgg cgaccgcggc ggccttgccc tcggagcggg gattcgaata attgacgacg
     1861 acgccggcgg ccccgtgctg ggccaggtgg atggcggtcg accggccgat ccccccggcg
     1921 gaccccgagc cggtgacgat cgcggccttg cccctcacat ccatcctgca acgctcgggt
     1981 ccattacctg acaggcgaca gccacgcttt gcggagccgg atcatcccgt cgggcacctg
     2041 cacgaacccg cggagccggg tggagatcag cttgtactcc ttgggaaagt acagccacac
     2101 atagggcacg tcgtcggcga ggatctgggt cacctctcgg tacgcccgcc gtcgctggct
     2161 catatcgttg aggacccgcg cagcgtccag gagcgtctcc atccgcgggt tattgtagcc
     2221 tgcgtagttg aacgagccga tgcccgacgc cgtcgtgaac gggtagatat cgaagtcggg
     2281 atccggacgc ccgctccatt cgatgagcgc cccctcttcg cgcaggtggc tgatcgcgtc
     2341 gatgatcgtt ccctcgtcga cgatttggat cttcatctcg atcccggcgt ccccggccat
     2401 ggcctggatc gcctgggcca ccgcctgccg ctgttgcccc ggctggttca gcagcgtaaa
     2461 cgtgaatccg cccgcgtgcc cggcggcctg gagcttctcc ttggccagcg cgacactgcg
     2521 cggcggcagc ttccagccgg gttcgtaggc cggggtgccg ttggggaaga acgcgtgcgc
     2581 gggatacgcg gctccctgca gcacgacgtt cgcgagggcg tcccggtcga tcgtggcggc
     2641 gaacgcctgg cggagcagct tattgtcgaa cgggggcctg gtgacattca acgcgatcgc
     2701 ggtccaggcg atggcccccc gctcgatcag tttgaagcgc gccccgggct gcgccgcctc
     2761 ccgggcgagc tcccgggcct gcggcagcgg cacgacgttg atgatgtccg catcccccga
     2821 cttgacgttc gcgacgcggg cgttgtcgtc ggtgatcgtg cgaaagacga tacgatccgc
     2881 gtagggcact cctttctccc agtactctgg attgcgttgc agcgtgatgt ggtccgcggg
     2941 aatcttctcc acgaacgaaa acggcccggt cccgacggga tggagcgcgt agttgaggcc
     3001 ctccttctgg gccgccgtcg gagacaccat catcccggcg cggtcggtga ggacatacag
     3061 gagggggctg tacggccgct caagggtgat ctggaccgtg gtggggtcga cggcggtcac
     3121 gctcg
//
LOCUS       DASQGH010000143         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51544, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000143 DASQGH010000000
VERSION     DASQGH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51544"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VEW91_02665"
     CDS             complement(<1..665)
                     /locus_tag="VEW91_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90522.1"
                     /translation="MENARIVLWTEGEAGPRFPLDERGITAAGGVIRKVACRDEADRI
                     AAAREAYVLVVSHAQIGEPLFAGAPKLVGLVRTGIGLDTVDIPGATRHGVCVAHVPDF
                     CYDEVADTAMTLLLAVARKVRLADRHVRSGSWAPSALLPMRRLRGQTLGLVAFGHIAR
                     MVAERARGFGLHVIAYDPYVDGPTMAALGVDKVELDTLLARSDIISVHTPLTEETRGL
                     IDQA"
     gene            complement(688..>1625)
                     /locus_tag="VEW91_02670"
     CDS             complement(688..>1625)
                     /locus_tag="VEW91_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYM90523.1"
                     /translation="ARPGAHDRIDGLAHVDKVINIDQSPIGRTPRSNPATYTKTFDLI
                     RELFALTPDARTRGFLQGRFSFNVRGGRCEACEGDGIVRIEMHFLPDVYVPCDVCKGK
                     RYNRETLEVRYKGKSIADVLDMSIDEALTFFEPIPRIRRKLRTLGDVGLGYVRLGQPA
                     TTLSGGEAQRVKLSTELSRRDTGRTVYILDEPTVGLHFADVARLLEVLHRLADAGNTV
                     VVIEHNLDVIKTADWIIDLGPEGGELGGEVIAEGSPEAVTEVPASYTGQFLRPVLRRA
                     AGREARLVAAGPRGRLRSGNGLAGQVPVARRGRAR"
BASE COUNT          221 a          614 c          560 g          230 t
ORIGIN      
        1 gcctgatcga tcagcccgcg ggtctcctcg gtgagcggcg tgtgcaccga gatgatgtcg
       61 ctgcgcgcga ggagggtgtc caactcgacc ttgtcgaccc cgagcgccgc catcgtcggg
      121 ccgtcgacgt acggatcgta cgcgatcacg tggaggccga acccgcgcgc gcgctccgcc
      181 accatccggg cgatgtggcc gaacgccacg agccccaggg tctggccgcg gaggcggcgc
      241 atcgggagca gggcgctcgg ggcccaggac ccgctccgga cgtgccggtc ggcgagccgc
      301 accttccgcg ccacggccag cagcagcgtc atcgccgtgt ccgcgacctc gtcgtagcag
      361 aagtccggga cgtgcgcgac gcagaccccg tggcgggtcg cgccggggat gtcgaccgtg
      421 tcgaggccga tcccggtgcg gacgagtccc acgagcttgg gcgcccccgc aaacagcggt
      481 tccccgatct gggcgtggga gacgaccagc acgtaggcct cgcgcgcggc ggcgatccga
      541 tcggcctcgt cccggcacgc gaccttccgg atcaccccgc ccgcagcagt gatcccccgc
      601 tcgtcgagcg gaaaccgcgg ccccgcctcg ccttccgtcc ataggacgat ccgggcattc
      661 tccatccgcg cccccctcgc gatcgaccta ccgcgcccgg ccgcgccggg ccaccggcac
      721 ctgtcctgcg agcccgttcc cgctccggag ccgcccacga gggcctgcgg cgacgagccg
      781 cgcctcccgg cccgccgccc ggcggagcac cgggcggagg aactggccgg tgtaggaggc
      841 cggaacctcg gtcaccgctt ccggagaccc ctcggcgatg acctcgcccc cgagctcgcc
      901 cccctccggg cccaggtcga tgatccagtc ggcggtcttg atgacatcga ggttgtgctc
      961 gatcaccacg accgtgttcc cggcgtcggc caggcggtgc aggacctcga gcaggcgcgc
     1021 gacatcggcg aagtggagcc cgaccgtcgg ctcatccaga atgtacacgg tccggcccgt
     1081 gtcccgccgg ctcagctcgg tgctgagctt cacccgttgc gcctcgccgc ccgacagcgt
     1141 cgtcgcgggc tggcccagcc ggacgtagcc caacccgacg tccccgagcg tgcgcagctt
     1201 ccgccggatc cgcgggatcg gctcgaagaa ggtgagcgcc tcgtcgatgc tcatgtcgag
     1261 gacgtcggcg atcgacttgc ccttgtagcg gacctcgagc gtctcgcggt tgtaccgctt
     1321 ccccttgcag acgtcgcacg gcacgtacac gtccgggagg aagtgcatct cgatccgcac
     1381 gatcccatcg ccctcgcagg cctcgcaccg ccccccgcgg acgttgaacg agaaccggcc
     1441 ctggaggaag ccccgcgtcc gggcgtccgg ggtcagggcg aagagctcgc ggatgaggtc
     1501 gaaggtcttc gtgtaggtgg ccgggttgct ccgcggggtc cgcccgatcg gcgactggtc
     1561 gatgttgatc accttgtcga cgtgggcgag cccgtcgatc cggtcgtgcg cccccgggcg
     1621 ggcgc
//
LOCUS       DASQGH010000144         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6290, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000144 DASQGH010000000
VERSION     DASQGH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6290"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VEW91_02675"
     CDS             <1..580
                     /locus_tag="VEW91_02675"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYM90524.1"
                     /translation="VRRQAVIAARAGVSQAQAGLRNAQSGYTVIAQRRQDLAAAQAAL
                     AQAQANLKYQQVIAAHNVITAPLGAVVQTKYVQQGEVVAAGTPLYTLIDLQDIWLRVY
                     IPEDQIGRVKVGEAALVTIDSFPGRIFQGRVIEISSHGEFTPGNVQTRGDRVKLVFSV
                     KIQLNNSDNSLKPGIPADAEIIVGGQEDRIPR"
     gene            577..1539
                     /locus_tag="VEW91_02680"
     CDS             577..1539
                     /locus_tag="VEW91_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90525.1"
                     /translation="MTATESPAILVQGLTRRFGETVAVKDLSFAVARGEIFALLGPDG
                     AGKTTTLRMLCGAFAPTSGTAVIDGIALPREIERVHERVAYMPQRFSLYEDLTVQENL
                     DFYAEIFGVPRDVRAERGRRLLEFSGLGSLTKRLAAELSGGMKQKLALACTLIHEPRV
                     LLLDEPTAGVDPASRREFWRILYQLNREGATILASTTYMDEADRCTTVGLLYEGELIS
                     AEDPRMMKRQMRGDVVEFIAEPQAAARRIIAHSAEVLSEAVIGDRFHVVVADADAAIP
                     TLTQRLIAQGVTVQGVRKVPASLEDVFVSIIAERRGGREGAAHA"
     gene            1532..2506
                     /locus_tag="VEW91_02685"
     CDS             1532..2506
                     /locus_tag="VEW91_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90526.1"
                     /translation="MPERGVAVHAHELTRTFGEFVAVDHLNFEIPTAAVWGFLGPNGA
                     GKSTTIRMLCGILSPTAGRAEVLGYDVTRDPERIKARIGYMSQRFSLYNDLTVEENLA
                     FYAGVYGLPRDEARERAGEWIGRAGLRGHERTLAGALSGGWKQRLAFGCAVLHRPEMI
                     FLDEPTSGVDPVSRREFWDLIAQFTEDGVTVMVTTHYMDEAEHCDTLAFIFGGRIIAM
                     GSPVEIKEQQMSGELLEILTPQYAEALEMLSARPEVREAALFGRAIHVTVEDSGAALP
                     VLREALANRGVTVDAIAPVAPSLEDVFVSLMERPGGPSESPASPAGQP"
     gene            2503..>3115
                     /locus_tag="VEW91_02690"
     CDS             2503..>3115
                     /locus_tag="VEW91_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90527.1"
                     /translation="MNLRRLRSIIRKEFTQLIRDRRTLITIITLPIMQLILYGYLSND
                     VTHIATAVFDESRTPESRTLLSAFANTTYVDLRYYTTGFDQVRRLIDGGRAKIGIWIP
                     PDYAARLRSGRTGQVEVIVDASEPTSARTVLAIASELGSVLSTQIVLQEQQRLFGAAG
                     ATLNARPVDIRARAWYNPTLENANFLVPGVIAIVLLFVTTFQAV"
BASE COUNT          515 a         1033 c         1069 g          498 t
ORIGIN      
        1 ggtcaggcgg caggcggtga tcgccgcccg cgccggcgtc tcacaggcgc aggcgggtct
       61 caggaacgcc cagagcggct acacggtcat cgcgcagcgg cggcaggacc tggccgccgc
      121 ccaggccgcg ctggcgcagg cgcaggccaa cttgaaatac cagcaggtga tcgccgcgca
      181 caacgtcatc accgcccccc tcggcgccgt ggtccagacc aagtacgtgc agcaggggga
      241 agtggtcgcc gccgggacgc ccctctacac gctgatcgat ctccaggaca tctggctgcg
      301 ggtgtacatt cccgaggacc agatcggccg cgtgaaggtc ggggaggcgg cacttgtgac
      361 catcgacagc ttcccgggcc gcatcttcca ggggcgcgtc attgagatca gcagccacgg
      421 cgaattcacg cccggcaacg tgcagacccg cggggaccgc gtgaagctcg tgttcagcgt
      481 gaagatccag ctgaacaact ccgacaacag cctgaaaccg gggatcccgg ccgacgctga
      541 gatcattgtc ggcggccagg aggatcggat accccggtga cggcaaccga gagtcccgcc
      601 attctggtgc aggggctgac ccggcggttc ggcgagacag tcgccgtgaa ggatctctcg
      661 ttcgccgtcg cccgtgggga gatcttcgcg ctgctgggtc cggacggcgc gggcaagacc
      721 acgacgctcc ggatgctctg cggggccttt gccccgacca gcggcacggc cgtgatcgac
      781 gggatcgctc tcccccggga gatcgagcgg gtccacgagc gcgtggcgta tatgccccag
      841 cgcttcagcc tgtatgaaga tctcacggtg caggagaacc tcgacttcta tgccgagata
      901 ttcggcgtgc cgcgggacgt gcgcgcggaa cgcggccggc ggttgctgga gttcagcggc
      961 ctcggctcct tgacgaagcg gctcgccgcg gagctgtcag gcggcatgaa gcagaagctg
     1021 gccctcgcct gtacgctgat ccatgagccc cgtgtgcttc tgctcgacga gccgaccgcc
     1081 ggggtcgatc cagcctcgcg tcgcgagttt tggcgcatcc tgtaccagct gaaccgtgag
     1141 ggtgcgacga tcctcgccag cacgacatac atggacgagg cggaccggtg caccacagtg
     1201 gggctcctgt acgaggggga gctgatttcc gccgaggatc cccggatgat gaagcggcag
     1261 atgcgcggcg atgtggtgga gttcatcgcc gagccgcagg ctgccgcgcg ccggatcatc
     1321 gcgcactccg cggaggtcct ctcggaagcg gtgatcgggg accggttcca cgttgtcgtc
     1381 gcggacgccg acgcggcgat ccccaccttg acccagcgcc tgatcgctca gggcgtgacg
     1441 gttcagggcg tgcgcaaggt ccccgcatct ctggaggacg tcttcgtctc catcatcgct
     1501 gagcggcggg gcgggaggga gggggccgcg catgcctgag cgtggtgtgg cggtgcacgc
     1561 ccacgaactg acccggacct tcggcgagtt cgtggccgtc gatcatctca acttcgagat
     1621 cccgaccgcg gcggtgtggg gcttcctcgg ccccaacggc gccggaaagt cgacgacgat
     1681 ccggatgctc tgtggcatcc tgagcccgac ggcaggacgg gcggaggtgc tgggctatga
     1741 cgtgacgcgc gacccggagc ggatcaaagc gcggatcggg tacatgtccc agcgcttcag
     1801 cctctacaac gacctgaccg tggaggagaa cctggcattc tacgccggcg tctacgggct
     1861 gccacgggac gaggcgcgcg agcgcgccgg tgaatggatc gggcgggccg ggctccgggg
     1921 gcacgaacgc accctagcgg gggcgctctc gggcgggtgg aagcagcggc tggcgttcgg
     1981 gtgcgcggtg ctccaccgcc cggagatgat ctttctcgac gagccgacca gcggggtcga
     2041 tccggtcagc cgccgcgagt tctgggacct gatcgcccag tttacggagg acggagtgac
     2101 cgtcatggtc accacccact acatggacga ggcggagcac tgcgacaccc tggcgttcat
     2161 ctttggcggg cggatcattg cgatgggatc ccccgtggag atcaaggaac aacagatgtc
     2221 gggggaactc ctagagatcc tcactcccca gtacgccgag gcactggaga tgctctcggc
     2281 ccggccggag gttcgggagg cggccctctt cggacgggcg atccacgtca ccgtagagga
     2341 cagcggggcc gcgctgcccg tcctccgtga ggccttggcg aatcgggggg tgaccgtcga
     2401 cgccatcgcc cccgtggcgc cttcgctcga agatgtgttc gtgtccctca tggaacgccc
     2461 cggcggtcct tcggaatctc ctgcctcgcc cgcgggtcag ccgtgaacct ccggcgtctg
     2521 cgcagcatca tccgcaagga gttcacccag ctcatccggg atcggcggac gctcatcaca
     2581 atcattacac tgccgatcat gcagctgatc ctctacggtt acctgagcaa cgacgtcacc
     2641 catatcgcca ccgcggtctt cgacgaatct cgcacgccgg agagccggac gctgctcagc
     2701 gcgttcgcca ataccaccta cgtcgatctc cgatactaca ccaccgggtt cgatcaggtg
     2761 cgccggctga tcgacggcgg acgggcgaag atcggcatct ggatcccgcc cgattacgcc
     2821 gcccgtctcc gcagcgggcg gaccgggcag gtggaggtga tcgtcgacgc ctcagagccg
     2881 acgtcggccc gcacggtgct ggccatcgcc agcgagctcg ggtccgtgct gtcgacccag
     2941 atcgttctgc aagaacagca gcggctgttc ggcgccgccg gcgccaccct gaacgcgcgg
     3001 cccgtggaca tccgcgcgcg ggcctggtac aaccccacgc tggagaacgc caacttcctt
     3061 gtgcccgggg tcatcgcgat cgtcctgttg tttgtcacga cgttccaggc ggtga
//
LOCUS       DASQGH010000145         4190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40249, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000145 DASQGH010000000
VERSION     DASQGH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40249"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VEW91_02695"
     CDS             <1..222
                     /locus_tag="VEW91_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYM90528.1"
                     /translation="EGLLDHPHYTRPATFRGHAVPEVLLSGHHEAVRRWRAREALRRT
                     LLRRPDLIDEAALDAEARALLAELRARGT"
     gene            297..638
                     /gene="rplS"
                     /locus_tag="VEW91_02700"
     CDS             297..638
                     /gene="rplS"
                     /locus_tag="VEW91_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYM90529.1"
                     /translation="MERLIAVERQHAKAQVPSFRAGDTVRVHIKVAEGGRDRLQAFEG
                     VVIGRRGGGLREAFTVRRISHGVGVERTFPVHSPRVEKIEILRTGRVRRAKLYYLREK
                     VGKETRVREKR"
     gene            714..1256
                     /gene="lepB"
                     /locus_tag="VEW91_02705"
     CDS             714..1256
                     /gene="lepB"
                     /locus_tag="VEW91_02705"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYM90530.1"
                     /translation="MIKRTAQIPQAVRTAIVETLDACLFAALLSLVIITFVVQAFYIP
                     SGSMEPTLMVDDRILVAKFVYRLEPVRQGDVVVFRYPLNPQRDFVKRVIGLPGDRVAL
                     KDGVVFVGGQALSEKGYTIKPDFGNYGPVTVPARQYFVLGDNRNNSEDSRFFGYVPRG
                     NIVGKATFIYWPLPRLGLVH"
     gene            1290..2078
                     /locus_tag="VEW91_02710"
     CDS             1290..2078
                     /locus_tag="VEW91_02710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYM90531.1"
                     /translation="MDRRSLTVAEVAARLARVTRPSAAWLRDLSADPRRGVRRLVARY
                     ERTRELLRAERRRLWGLYREERGRRRAGQIVAGVDEVGCGCLAGPVVAAAVILTDAPP
                     IAGLDDSKRLTPASRERLNDEIRSRALAVAVAMASVEEIARLNIRGAARLAMHRAVES
                     LYPSPDFALVDGRDRLPPGVDHATIVRGDAACACIAAASIVAKVARDRLMQDLDAAFP
                     GYGFARHKGYGTAEHLAALLRFGPCPAHRGAFLPVAQTALFEWL"
     gene            2075..2440
                     /locus_tag="VEW91_02715"
     CDS             2075..2440
                     /locus_tag="VEW91_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYM90532.1"
                     /translation="MNDARQRAGRVGEDAAVEALRTRGYRILERNVRLRHGELDVVAE
                     EAGELVFVEVKARRSGTHGTPAEAVGARKQRALARLALGYLVRRRLGERACRFDVVEV
                     WLPPAGPPRVEILRDAFRP"
     gene            2491..4053
                     /locus_tag="VEW91_02720"
     CDS             2491..4053
                     /locus_tag="VEW91_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HYM90533.1"
                     /translation="MITRAKVYRQELSPVSFLERAGDVHAERIAVQDGDRAFTYRSWR
                     GRSRCLASALRRAGLEHGDRVAFLALNSEPLLLAHMGVPQAGGVLVAVNTRLNADEVR
                     YIVEHSGPRFVFYSPELRPQLAGLPAGLRRFEIGKEFEDLLATGSDEDPGPPLLDEEE
                     TIAIDYTSGTTGRPKGVMYTHRGAYLNALAMMIEHRLGSASTYLWTLPMFHCNGWTFT
                     WGLSAVGARSVCIPRIDPPQIWRLLDEGGITHFCAAPTILAMLANHPAAHRLARPVRV
                     FTAAAPPSPTILAQMAALGFEVDHVYGLTESYGPFTINVAPPGVEGLSPEERARLKAR
                     QGVAHVTAGAQRIVDDQMRDVPAAGRTMGEVIYRGNVVMKGYFAQPEATEEAFRGGWF
                     HTGDIGVMHPDGQIELRDRKKDIIISGGENISTIEVEQAVVSHPAVLECAVIAVPDET
                     WGEVPKAFVTLKPDQTATAEEIIAHCRARIAHFKCPKAVEFRELPKTSTGKVQKYVLR
                     EHEWRGHEKRIH"
BASE COUNT          615 a         1429 c         1515 g          631 t
ORIGIN      
        1 gaggggctcc tcgaccatcc gcactacacg cggcccgcga cgttccgcgg gcacgccgtg
       61 cccgaggtgc tgttgagcgg gcatcacgaa gcggtccgcc ggtggagagc gcgcgaggcg
      121 ctgcggcgga ccctcctccg ccgtcccgac ctcatcgatg aggcggcgct cgacgccgaa
      181 gccagggcgc tgctcgcgga actgcgtgcg cggggcacgt aaccggcgcg ttcttgtgac
      241 cgtcgttgcg agcgtggtat aatgggccac cgtgatcacg agaggggaac cggatcatgg
      301 agcgactgat cgccgttgag cgccagcacg ccaaggccca ggtgccgtcc tttcgggccg
      361 gcgacactgt gcgggtccac atcaaggtcg cggagggcgg acgggaccgg ctccaggcct
      421 tcgagggcgt ggtgatcggc cgccgcggtg ggggactgcg cgaggcgttt acggtccggc
      481 gcatctccca cggggtcggg gtcgagcgaa cgttcccggt ccactcgccc cgggtggaga
      541 agatcgagat cctccgcacg gggcgggtac gacgggcgaa gctctactat ctccgcgaga
      601 aggtcgggaa ggagacccgc gtcagggaga aacggtaggc ctgcacattg agctcgctga
      661 gcattccgac cgtcatcctg agcgtttcgg cgtttctcct cgcgtctcgg attgtgatca
      721 aacgcacggc ccagattccc caggccgtgc gcaccgccat cgtcgagacc ctcgacgcgt
      781 gcctcttcgc cgccctgctc agcctggtca tcatcacctt cgtcgtccag gcgttctaca
      841 ttccgtccgg gtcgatggaa ccgaccctga tggtcgacga tcgcattctg gtggccaagt
      901 ttgtctatcg tctggagccc gtgcgacagg gcgatgtcgt cgtgttcagg tatccgctca
      961 atccccagcg cgacttcgtg aagcgcgtca tcggtctgcc cggggaccgc gtggcgttga
     1021 aggacggggt cgtgttcgtg ggcggccaag ccctctcgga gaagggatac acgatcaagc
     1081 cggacttcgg gaactacggc ccggtcaccg tgcccgcgcg ccagtacttc gtgctggggg
     1141 ataaccgcaa caacagcgaa gacagccggt tctttggcta tgtcccgcgg ggaaacatcg
     1201 tcgggaaggc gaccttcatc tactggccgc tgccgcgtct cgggctcgtc cactagtgcg
     1261 cggacgcggc cggccgtgcc gctgaggccg tggaccggcg atccctgacg gtggcagagg
     1321 tcgccgcccg cctcgcccgc gtcacgcgtc cgagcgcggc gtggctccgg gacttgagcg
     1381 ccgatccccg ccggggcgtc cgccggctgg tggcccgcta cgagcgcacc cgcgagctcc
     1441 tccgcgccga acggcggcgg ctgtggggac tgtaccgcga ggagcgcggc cggcgccgcg
     1501 ccgggcagat cgtcgccggg gtcgatgagg tgggctgcgg gtgcctcgcc ggccccgttg
     1561 tcgcggcggc ggtgatcctc accgatgccc ccccgatcgc cgggctcgac gacagcaagc
     1621 gcctcacccc cgcgtcgcgc gagcgcctga acgacgagat ccgctcgcgc gccctggccg
     1681 tcgccgtcgc catggcgtcg gtcgaggaga tcgcccgctt gaacatccgc ggcgccgcgc
     1741 ggctggcgat gcaccgcgcg gtcgagtccc tctacccgtc ccccgacttt gcgctcgtcg
     1801 acgggcgcga ccgcctcccg cccggcgtgg atcacgcgac gatcgtgcgg ggcgacgcgg
     1861 cgtgcgcctg catcgccgcg gcctccatcg tcgcgaaggt cgcgcgcgac aggctcatgc
     1921 aggacctgga cgccgccttt cccgggtacg ggtttgcccg ccacaaaggg tacggcaccg
     1981 ccgagcacct ggcggcgctc ctccgattcg ggccgtgtcc cgcgcatcgg ggcgcgttcc
     2041 tccccgtggc gcagaccgcg ctgttcgagt ggctgtgaac gacgcgcggc agcgcgccgg
     2101 gcgggtgggg gaagacgccg cggtcgaggc gctgcgcacc cgcggatacc ggatcctcga
     2161 acgcaacgtc cgcctgcggc acggcgaact ggatgtggtc gcggaggaag cgggcgagct
     2221 cgtctttgtg gaggtcaagg cgaggcggtc ggggacccac ggcaccccgg cggaggcggt
     2281 tggggcgcgc aagcagcgcg cgctcgcccg gctggccctg ggctacctcg tgcgccggcg
     2341 gctcggcgag cgggcgtgcc ggttcgacgt cgtggaggtc tggctcccgc ccgcggggcc
     2401 cccgcgggtc gagatcctcc gcgacgcgtt ccgtccatga gccgtcgagc tcgatcgcgc
     2461 ggtccacgcc gcggcgaagg agggtcagcg atgatcaccc gggccaaagt gtaccggcag
     2521 gagttgtcgc cggtgagctt tctcgagcgg gccggagacg tccacgccga acggatcgcc
     2581 gtccaggacg gggatcgggc gtttacctat cgcagctggc gggggcgatc ccggtgcctg
     2641 gcctccgcgc tcaggcgcgc cggcctcgag catggcgacc gggtcgcgtt cctggcgctc
     2701 aactccgagc ccctgttgct cgcccatatg ggggtccccc aggccggggg cgtcctggtg
     2761 gcggtcaaca cccggctcaa cgcggatgag gtccggtata tcgtcgagca ctcggggccc
     2821 cggttcgtgt tctactcccc cgagctccgc ccgcaactgg cggggctgcc cgccgggctc
     2881 cggcgcttcg aaatcgggaa ggagttcgag gacctgctcg cgaccggcag cgacgaagat
     2941 ccgggcccgc cgcttctgga cgaagaggag acgatcgcga tcgactacac ctccgggacg
     3001 accggccgcc ccaaaggcgt catgtacacc caccgcggcg cctacctcaa cgcgctcgcg
     3061 atgatgatcg agcaccggct cggcagcgca tcgacgtatc tgtggacgct gccgatgttc
     3121 cactgcaacg ggtggacgtt tacctggggc ctgtcggcgg tgggggcgcg gagcgtgtgc
     3181 attccccgga tcgatccccc gcagatctgg cgcctgctgg acgagggagg gatcacccac
     3241 ttctgcgccg ctcccaccat cctggcgatg ctggccaacc atccggccgc tcaccggctc
     3301 gcccggccgg tccgggtctt caccgcggcc gcgccgccgt cgccgaccat cctcgcccag
     3361 atggcggcgc tcggcttcga ggtggaccac gtctacgggc tgaccgagtc ctacgggccg
     3421 ttcacgatca atgtcgcgcc gccgggggtg gagggcctga gcccggaaga gcgggcccgg
     3481 ctcaaggccc gccagggcgt ggcgcacgtc accgcgggcg cgcagcggat tgtcgacgac
     3541 cagatgcggg acgtgccggc ggccggccgc acgatgggcg aggtgatcta ccggggcaac
     3601 gtcgtcatga aggggtactt cgcgcagccc gaggcgaccg aggaggcgtt ccggggcggc
     3661 tggttccaca ccggagatat cggcgtgatg catcccgacg ggcagatcga gcttcgggac
     3721 cgcaagaagg acatcatcat ctccggtggc gagaacatca gcacgatcga ggtggagcag
     3781 gcggtggtca gccaccccgc ggtgctggag tgcgcggtga tcgccgttcc ggacgagacc
     3841 tggggcgaag tcccgaaggc gtttgtcacg ctcaagcccg atcagaccgc gaccgccgag
     3901 gagatcatcg cgcactgccg cgcgcgcatc gcgcacttca agtgccccaa ggcggtggag
     3961 ttcagagagc taccgaagac ctcgaccggc aaggtgcaga agtacgtgct ccgcgagcac
     4021 gagtggcggg ggcacgagaa gcgcatccac tgagcgcacc gcagcgttgg aggcgtcggt
     4081 gcgagccggg tgagagcgcg tctcgtcgag gcgcgccaac agtagttctc gtcagcgagg
     4141 agtgcacaat gaaggcgaag gctgcggtgt tgttcgaggt cggccggaaa
//
LOCUS       DASQGH010000146         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48720, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000146 DASQGH010000000
VERSION     DASQGH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48720"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2124
                     /gene="gyrA"
                     /locus_tag="VEW91_02725"
     CDS             <1..2124
                     /gene="gyrA"
                     /locus_tag="VEW91_02725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HYM90534.1"
                     /translation="GNFGSVDGDPPGAMRYTEARLSRMAMELLADIDKETVPFMPNFD
                     QSLREPAVLPSRVPSLLMNGATGIAVGMATNIPPHNLGELVDALVAAIDDPGLSQEAL
                     LKIVRGPDFPPGGLILGREGIHQAYTEGRGSIAVRAKTAFEELRGGRVAVVITELPYM
                     VNKAALVERIAELVREKKLSGVSDLRDESDRQGMRVVVELRRDVNPQIVLNQLFKHTQ
                     LQTTFGAIMLALVDGVPRVLTLKQLLAHYLDHRKVVVTRRTEFELKKAKARAHILEGL
                     KVALKSLDEVIALIRKAKDVPAARAGLVKRFSLSEAQADAILEMRLQRLTALERDKVD
                     DEYKELIKAIAYYEDVLHDERKVLAIVKTELLEVKGRYGDARRTRITGKDAELEEEDL
                     IPDADVVITLTHNGYIKRLLLETYRRQHRGGRGVMGMGTKEEDYVEVATVTTNHAFVL
                     FFSNRGKVYRVKAYEVPEAARMAKGTSLVNLVNVAQGERINAIIPIRSFEDAGFLFFA
                     TKHGYVKKTGLMEFINAKRAGIVAITLGKDDELVAVRFTDGKEEVILATRRGQAIRFK
                     EANVREMGRAARGVIGITLRRDDAVVGMAKSGEGRYLLVATELGRGKRTLLSQYRLQR
                     RGGSGVRSIRLTPHTGNVSAIRAVNDQDEVLIITAQGIVSRLLVKDISTQGREAQGVR
                     LQRLDEGDRVSAIAPIAVADAAAET"
     gene            complement(2140..>2488)
                     /locus_tag="VEW91_02730"
     CDS             complement(2140..>2488)
                     /locus_tag="VEW91_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HYM90535.1"
                     /translation="GAIWYSPAVFCTTWMARVGKKPEDLQTGMVPRSYGLMFLAALVL
                     AFVLVTVIRAAHVGTAMGGIKVGIAVWIGFVLTTSSGLVIFEGRPVKLYWINNGYHAV
                     TLVINGAILAAWA"
BASE COUNT          461 a          815 c          857 g          355 t
ORIGIN      
        1 ggaaacttcg gcagcgtgga cggcgatccg cccggggcga tgcggtacac cgaggcccgg
       61 ctctcgcgga tggcgatgga actgctcgcg gacatcgaca aagagaccgt ccccttcatg
      121 cccaacttcg accagtccct ccgggagccc gccgtcctcc cgagccgcgt ccccagcctt
      181 ctgatgaacg gcgccaccgg gatcgcggtg gggatggcca ccaacatccc tccccacaac
      241 ctgggcgagc tcgtcgatgc gctcgtcgcc gcgatcgacg accccggcct ctcccaggag
      301 gcactcttga aaatcgtgag ggggccggat ttcccgcccg gcggtctcat cctcggtcgc
      361 gaagggatcc accaggccta caccgagggg cgggggagca tcgccgtccg cgccaagacc
      421 gccttcgagg agctccgggg cgggcgggtg gcggtggtca tcaccgagct cccgtacatg
      481 gtgaacaagg cggcgctcgt cgagcggatc gctgagctgg tccgggagaa gaagctcagc
      541 ggggtgagcg acctgcggga cgagagcgac cggcagggga tgcgcgtggt cgtcgaactc
      601 cgccgcgacg tgaacccgca gatcgtgctc aaccagctgt tcaagcacac ccagctccag
      661 accacgttcg gcgcgatcat gctcgcgctc gtcgacggcg tcccccgggt gctgacgctc
      721 aagcagctgc tcgcgcacta cctcgaccat cgcaaggtcg tggtgacccg gcggaccgag
      781 ttcgagctca agaaggccaa ggcccgggcc cacatcctcg agggtctcaa ggtcgcgctc
      841 aagtcgctcg acgaggtgat cgcgctcatc cggaaggcga aggacgtgcc ggccgcccgg
      901 gccgggctcg tgaagcggtt ctcgctcagc gaggcccagg cggatgcgat cctcgagatg
      961 cggctccagc ggctcaccgc gctcgagcgt gacaaggtgg atgacgagta taaggagctg
     1021 atcaaggcga tcgcctacta cgaggacgtg ctgcacgacg agcggaaggt gctcgcgatc
     1081 gtcaagacgg agctgctcga ggtcaagggg cgctacgggg acgcccggcg gacccggatc
     1141 accggcaagg acgccgaact ggaagaggaa gatctgattc cggacgccga cgtcgtgatt
     1201 accctgacgc acaacggcta catcaagcgg ctgctgctcg agacctaccg gcggcagcac
     1261 cgcggcggcc gcggcgtgat gggaatgggg acgaaggagg aggactacgt ggaggtggcg
     1321 accgtcacca ccaaccacgc cttcgtcctg ttcttcagca accgggggaa ggtgtatcgg
     1381 gtcaaagcgt acgaggtgcc ggaggcggcc cggatggcca aggggaccag cctcgtcaac
     1441 ctcgtcaacg tcgcccaggg cgagcggatc aacgccatca tcccgatccg ttcgtttgag
     1501 gacgccggct tcctgttctt cgccacgaag cacgggtacg tgaagaagac ggggctcatg
     1561 gagttcatca acgcgaagcg cgcggggatc gtggcgatca cgctcggcaa ggacgatgag
     1621 ctggtcgcgg tgcgcttcac cgacggcaag gaggaggtca tcctggccac ccgccgggga
     1681 caggcgatcc gcttcaagga ggccaacgtg cgggagatgg gtcgagccgc ccgcggggtg
     1741 atcggcatta ccttgcgccg ggacgatgcg gtggtgggca tggcaaagag cggggaggga
     1801 cgctacctcc tcgttgcgac cgagctgggg cgcggcaagc gcaccctgct gagccaatac
     1861 cggctgcagc ggcggggcgg ttccggcgtg cgcagcatcc gcctgacgcc tcacaccggg
     1921 aacgtctccg ccatccgtgc ggtgaacgat caggacgagg tgctgatcat caccgcccag
     1981 gggatcgtga gccggctgtt ggtcaaggac atctcgacgc aggggcgcga ggcgcagggg
     2041 gtccgcctcc aacggctcga cgaaggcgac cgcgtgtccg cgatcgcccc gatcgcggtc
     2101 gcggacgccg ccgccgaaac ctagtgcccg cgatggaggt caggcccagg ccgccaggat
     2161 cgcgccgttg atcaccagcg tcacggcgtg gtacccgttg ttgatccagt agagcttcac
     2221 gggtcgaccc tcgaagatca ccagaccaga gctcgttgtc agcacgaacc cgatccacac
     2281 ggcgatcccg accttgatcc cccccatggc cgtgccgacg tgtgccgcgc gaatgacagt
     2341 gaccagcacg aacgcgagga ccagcgccgc caagaacatc aggccgtacg atctcggcac
     2401 catgcctgtc tgcagatcct cgggcttctt gccgacgcgc gccatccacg tggtgcagaa
     2461 tacggcgggc gagtaccaga tggcgccg
//
LOCUS       DASQGH010000147        14699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43023, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000147 DASQGH010000000
VERSION     DASQGH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43023"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3223)
                     /locus_tag="VEW91_02735"
     CDS             complement(<1..3223)
                     /locus_tag="VEW91_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYM90536.1"
                     /translation="MAAWTAFAVLAITVFAGLLWHRYLSSGRFATDVHRVVVRQLEAS
                     LHREVTLGGVSGSLLSGVVLSDLRIAERGGFAHGVAFSSEEVRLSFDWRSLLAARPVA
                     VGGIIQVDLRHPHLALSKSAEGAWNVHDLLGSHGPPAAGRLRGRVLIHDGTLEFSDAE
                     NAPASPFAVTFDRVNGDVEVQGGEVAIALTGWSRDSEAVRLSGRYRPGWRTSAFDLTA
                     ANASAARWGPYFVHLPELGWARGRFAAQVHLALTESPSDSALDYTAAIRLHDTDVEYR
                     PAHLWLRGVSGPITVDGAHAETPGLLLHADGSPLSADGTLLFAGGPWVQLRLRSPALD
                     LATVQALFFPHARLTITGQAGAEIQVSGPVQALDLDGEITGATGRLNGQGFEGLHARM
                     QYAAGTVTVTSLRSSVAGGQLSGDLVLAVANGTPSYLFSGTTSDMDVGALASAGLSGL
                     GAVRGRISGHVVGVGTGPRVQFMGDVTLGPGDVRGVPFDRMHAIFWQGDSGVVDLDYL
                     SGRIGGATIYGSGRIGADGALDLDILAHDLSFADIGARGPLRNAPLDGRADVIGRATG
                     TTAAPVFSGTVTATHGRMGPIAFAQARGSLTAGPTGVTALRLDLLNGGARYQVSGGIA
                     FDPLGASNLQLEAEDVDAQWLTGALPLAPDVTGLLSGTLVIDGPLPRASVAGRVVLQH
                     GSVGGQRIDHAEVHLTPESGRLRIADAEARINGARVFATGTIDPGGSLDLRGGAEDVQ
                     LADLSVALGVPISAQGILALSGEVHGTLQDPRISGEIAAPDIEVGGQAFAASGAIDYE
                     AGMLQLSGLQLAQGASRYRLSGTIRLGAHPSASLALDVEGGRVATVLGAAGIRPPAPL
                     DGAVDGRIELSGPVDDPSAHLSLRLRDARFGAYAIGDGIADLTLTHQAIDIDRFEIHP
                     AQGQVAAKGRVDLRGTSAVEVSAQDLNPDFLRPFFQLDRPLQGRLNFTLQLSGPLGDP
                     QAGVSLEAFDVGVADAQADQIAALAYYSAGTLHLEQGVISKGPHKLVVTGTLPVDRGT
                     FTLDAHAPLQLQLRLQDADLSFLSLFTPKITDATGTVAGQVYVA"
     gene            complement(3271..5025)
                     /locus_tag="VEW91_02740"
     CDS             complement(3271..5025)
                     /locus_tag="VEW91_02740"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HYM90537.1"
                     /translation="MAGRVGRWGAAGLAAGLAAGLLGAPPPLVASPAVLPSAQITAGM
                     TGIGKTVILGTTISEFQVIVLGVLRNAGPAGDLVLFRAFGSAIQATGGLASGMSGSPI
                     YLGGKLAGAFSYSFQFADPMVGLFTPIEDMLRAMPRAVRVPPPGVYAVTPFALGGRTI
                     RRGELRASPDPTPTAPDTATAVPAATPLFVSGLGYEATEELARSLQPMGLLPMAGPGM
                     VSLPPTIRLEPGSAIGVALMQGDLAAYAIGTLTYRDGNRILAFGHPFTDNGRTEFLLT
                     NAIIFQTVRAMNHNLKVGAAGAPVGIISEDRPAAVGGTLGVLPRMFGARVAVHDSDAG
                     TDRRFAFQVVNSKELAPVLVALGARGAVERALNRSGEGTAEVRIALGGRGLPRRVERA
                     NIFYSSTDIASRALIEVPQALRLLFENDFADAGPTDISIDIRITKARQTGTLTEAEMP
                     KGPVSPGGTVRVRVSVRPFREAPVTRDIEVVVPADFPEGPAMLIVRGGSAAPQPATGA
                     LSSPQQGPAAINTLADAIAAFEEGEKSTDVVVDLVSGARPPAGSAAGPAHVSTRWTTP
                     WVLTGRFQTPIVIGRGAR"
     gene            complement(5078..6769)
                     /locus_tag="VEW91_02745"
     CDS             complement(5078..6769)
                     /locus_tag="VEW91_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90538.1"
                     /translation="MTGYGLTVLQGTTITRFGIQVLGILKGGPTSDLILFRATGPVIQ
                     EAGGTAAGMSGSPLYVDGRVIGGLSYGYHFAGKDADLSLATPIEEMLKLLAPPPPGAS
                     DTAWGRPPRVFEAAAPIPTPGGVVSRVVLMRSTADAAAYNAHPLPRSVAVAPTAVPMF
                     TGGFSPRALALLARQLNRYNVVPMQGYGGSRQFTPPAVEPGSSIGIELARGDIEVGAI
                     GTVTYRRGGQVLAFGHPLLNAGWATMPLAAAWIDTVVRSMDFPFKEGSLGPLIGSTTQ
                     DRSTGVAGEVGRFPRMFGVRVRVRDGAGPLRETAAQVVRRGDLAESLVPTVVLSAVQR
                     SLDRVSSGSARVRIGLRALGIAHEIVREDLAFDAADIATAAVLDVPGATQLLFGNFFK
                     TLEPIDMTIDIAVQNRPNTALLVAADTPTRTLTRGQRVRVEIGLVPFGNEDPVARTVE
                     FTVPRDFPRGPAFLLVGSAGGLNDAAQIPTDQKFQLLAGLQGSPQGTAASLDDAVDQF
                     EHAGKNTDVLVTLLPAPVLQAVGGNANPAFDPSTGTSFSTEWVVLGRFQIPMVVE"
     gene            complement(6990..7310)
                     /locus_tag="VEW91_02750"
     CDS             complement(6990..7310)
                     /locus_tag="VEW91_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90539.1"
                     /translation="MSLSRWALCMTLVVVGLTAVLSPPVPAQQANVLVRVVISGEVLP
                     LQLAKVGQVVRQGDPLVFLRGTSSGAAVPGAVASVDGQVVQVMVRPGDRVNIGDVVAV
                     IQPK"
     gene            complement(7363..8577)
                     /gene="wecB"
                     /locus_tag="VEW91_02755"
     CDS             complement(7363..8577)
                     /gene="wecB"
                     /locus_tag="VEW91_02755"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HYM90540.1"
                     /translation="MVVFGTRPDAVKMAPVVRALRTHPVEFTPIVAVTAQHREMLDQV
                     LSLFAITPDHDLGIMTEQQSLVDITVRTLEGLSALLPKVAPDLVLVQGDAAPSFVGSL
                     AAFYRRIPVGHVEAGLRTADKYQPFPEEMYRRMTTVLADLHFAPTSAARENLKAEGVP
                     GDRILVTGNTVIDALLEVVRRDRPPADPRLRGVLAGRGRRVLLLTTHRRENWGEPQRR
                     IFLAVREILDRFPDLDLVFPVHPNPVVSRPAREILGGHPRAHLSEPLDYATNVACMKA
                     ATVILTDSGGIQEEAPSLGRPVLVLRETTERPEGIAAGTARLVGTDHTRIVQETSRLL
                     TDPRAYARMSLARNPYGDGHAAERIVGGLRRYFGFARNLPREFSVHIRGRGGAAALAG
                     RSGSRRARRSYV"
     gene            complement(8601..9722)
                     /locus_tag="VEW91_02760"
     CDS             complement(8601..9722)
                     /locus_tag="VEW91_02760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HYM90541.1"
                     /translation="MTISRFLVAGAIGLVVAYALTPLVEWAAIRVGALAQPGGRHIHR
                     VPVPRLGGIGVYLAFLVAVLAGLPLERSVRITLEAHHVVLTVPFTPQFDRPVIGVLCG
                     ATLITILGIIDDIWGVSPMEKLFGQLVAAIVPLSFGVGMDVLTNPLGGMLFLGPFGLA
                     LTVAWIVALCNVMNLIDGIDGLATGIAAIAGGTVLIASYQRGDAGTAMLAAAFVGSTL
                     GFLPYNFNPARIFLGDSGSMLLGYLLGSLSVLGTYKSYTALSLLVPLAALGVPVFDTA
                     LAIARRWRTGRPIFQADTEHLHHRLLQRGLSQRQTAVVLYLVTGVLGAGALLASGVHR
                     FALVAVLGALLAALAFGARRTGLLTPPSPSARGRTEVPR"
     gene            complement(9745..10725)
                     /locus_tag="VEW91_02765"
     CDS             complement(9745..10725)
                     /locus_tag="VEW91_02765"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYM90542.1"
                     /translation="MTGAGLRVFSGTGNPDLARGIAAALDLPLGAINVFRYADGEIGV
                     RIEESVRGEDVFLVQPTCPPVSENLMELLVITDAVRRASASRITAVIPYFGYARQDRK
                     IKPREPISAKLVANLLTTAGAHRVLTLDLHAGQMWGFFDIPLDHLPCRMILGDYFRGL
                     GLENLVVVSPDIGGVKRAREFAEYLEAPLAIIDKRRDRPNQVADVVHVIGKVYRRTAI
                     LVDDIIDTGGTLVMGAEALVRRGVHEVYACCTHAILSPPATTRILRSPIRQLVVTDSI
                     PVPQEKRTAKTVVLSVAGLLAEAIRRIHADQSVSELFAQPRVMQPVAEKE"
     gene            complement(10722..12080)
                     /gene="glmU"
                     /locus_tag="VEW91_02770"
     CDS             complement(10722..12080)
                     /gene="glmU"
                     /locus_tag="VEW91_02770"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HYM90543.1"
                     /translation="MTQLAAVILAAGRGTRMKSDLPKVLHPLCGRPMVLYAVETVRRS
                     GVARPLLVVGKDAAAFRPVIGTTLPYVVQRRPLGTGHAVAQALPRLARQRLVYVVYGD
                     MPFVSPTTLRALREALRASGARAAVATGVLAGAHHFGRILRDGQRRFQRIVEDRDATP
                     GERAVQEVNVGVYCFRVPELRRALERVRPNNHQREYYLTDAVNLLGEEDGGVATVDVP
                     DPDEMIGINSREELSQAERCMRRRILTRVMESGVTVIDPATTFIDETVRIAQDTVVHP
                     LTFVTGRTVVGRACAIGPGARIHDSVIGRRARIRDSSLEGARVGDGSVVGPYAHLRPG
                     TVVGRDVEIGNYAEMKQVRVGDRTKVHHKCYLGDAWIGTDVNIGAGTITCNYGLDRRK
                     HRTTIGSGAYIGSDSMLVAPVRIGRGAITGAGAVVTKDVPPRSVVVGVPARVIRVLGA
                     KR"
     gene            complement(12246..>14699)
                     /locus_tag="VEW91_02775"
     CDS             complement(12246..>14699)
                     /locus_tag="VEW91_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYM90544.1"
                     /translation="IIAIDPKGNVQMWSPAAERILGWSADEVLARPFPFVLEDGGAGP
                     RTLAQALRESGPWNGTRVSSHRKNGAALEVRVWAAPLNGPTQSWGGFVIAIEEVAKRA
                     RVEDAGGRRGAHPETLQAIVASALRATDLQGLFEETIDRTLGALDVMMGAAWIGQVSV
                     ARGIASDLGTALAQIAPPDAGGARKALCVADWRAAAPADAPKGIAEQFMARGIRASII
                     TSFGTKGGLAGGLVVASAAPRPWTPDDLAAVESVGEVLAAAAPRLRLFEETQQRLRLF
                     DRFRTTGRTLHRRATVAEVAETIGDGARVLGSADRTAVYLCQPDGTLACPWSVGLSHA
                     YVDQVLRHAKTLVGGRVMDGAKPDLLEISGRGIDGSAPVLYPDVLALSPAVAVPRLTQ
                     AEGYRALASWPLVHEGAVLGLIGCYYDAPRTWSAPEQKVFQGFCEEAAFALHNAGARE
                     AQMKRTADLEVLFELTRLLRAARNTGEIYPILVGRAMDLMHADSGVLTLLAGERAEFE
                     CVFAAGLPPEVRGSTFPLPGSPFERVVKTGTTFQTPNFGGDPLPVWLSSFRALGPAVI
                     VPVRSEQEIIGVFCLGRKRRSDASPFAEAEARLLEGIAEIGGNAIRRARLFENLEQSY
                     MQMVISLARTMDARDTYTSGHSERISEWAEAVARDLGCGEGEVQDVRWGALLHDIGKI
                     GVPDAILRKPGDLTGQEWGIMRRHPVIGEEILTPIGRMRGVAALVRHHQEKWDGTGYP
                     DGLKGEEIPLGARILAVCDAYSAITDERPYQKARDHAEAVAELHQAAGQQFDPRVVEA
                     FCRVIEREREREKAGQRPR"
BASE COUNT         2282 a         5314 c         5111 g         1992 t
ORIGIN      
        1 ccgcgacgta gacctgcccc gcgaccgtcc ccgtggcgtc cgtgatcttg ggggtgaaca
       61 gcgagagaaa gctcagatcg gcgtcctgaa gacgcaactg cagttggagc ggcgcgtggg
      121 catccagcgt gaaggtgccc cgatcaaccg gcagcgtccc cgtcaccacg agtttgtggg
      181 ggcccttgga gatcaccccc tgctcgaggt gcagggtccc cgccgagtag tacgccaggg
      241 cggcgatttg gtcggcctgg gcatcggcaa ccccgacatc gaacgcctcg agcgagacac
      301 cggcctgggg atccccgagg gggccgctga gctgcagcgt aaagttcagc cgaccctgga
      361 gcggccggtc cagctgaaag aagggccgga ggaagtccgg gttgagatcc tgggcggaga
      421 cctcgaccgc gctcgtgccg cgcagatcca cccggccctt cgccgccacc tggccctgcg
      481 ccgggtggat ctcgaagcgg tcgatgtcga tcgcctggtg ggtcagggtc aggtcggcga
      541 tcccgtcgcc gatggcatac gcgccgaacc gcgcgtcccg cagcctgagc gacagatggg
      601 cggaaggatc gtcgaccggg ccggagagct cgatgcgccc gtcgaccgcc ccatcgagcg
      661 gcgcgggggg cctgatcccg gcggccccga gcaccgtcgc cacccggccg ccttcgacat
      721 cgagcgcgag gctggcggag ggatgcgcac ccagccggat cgttccggag agccggtagc
      781 gcgacgcccc ctgcgccagt tgcagcccgg agagctggag catccccgcc tcgtagtcga
      841 tggcgcccga cgcggcgaac gcctgcccgc cgacctcgat atccggggcg gcgatctcac
      901 ccgagatccg ggggtcctga agggtgccgt gcacctcgcc ggacagcgcc aggatcccct
      961 gcgccgagat cggcaccccc agcgccacgc tgaggtccgc cagctggacg tcctccgccc
     1021 ctccgcgcag gtcgagagac ccgcccgggt cgatcgtccc tgtggcgaac acgcgggcac
     1081 cgttgatcct ggcctcggcg tctgcgatgc gcaggcgccc gctctcgggg gtcaggtgga
     1141 cctcggcgtg atcgatccgc tggccaccga cgctgccgtg ctggaggacg acccgtccgg
     1201 ccaccgacgc acgggggagc ggcccatcga tgacgagggt gccggagagc aggccggtga
     1261 cgtcgggcgc cagcggaagg gcaccggtca accactgcgc gtcgacgtcc tcggcctcga
     1321 gctggaggtt ggatgccccg aggggatcga acgcgatccc gccgctcacc tggtaccggg
     1381 cgcccccgtt gaggagatcc aggcgaagcg cggtcacccc cgtgggaccg gccgttaggc
     1441 tcccccgcgc ctgcgcgaag gcgatcgggc ccatgcgccc gtgggtggcg gtgaccgtcc
     1501 ctgagaacac gggcgccgcg gtcgtccccg tcgcgcgtcc gatgacatcg gcacggccgt
     1561 cgagcggcgc attgcggaga ggcccgcgcg cgccgatgtc tgcgaaggac aggtcgtggg
     1621 ccaggatatc gaggtcgagc gcgccatccg ccccgatccg gccggagccg tagatcgtgg
     1681 cgccgccgat ccggccgctc aggtagtcca gatccacgac ccccgagtcg ccctgccaga
     1741 agatcgcgtg catccggtca aacggcacgc cccggacgtc gccgggtccg agcgtcacgt
     1801 cccccatgaa ctgcacgcgc ggcccggtcc cgaccccgac cacatgcccg gagatccggc
     1861 cccggaccgc gccgaggccg ctcaggccgg cggaagcgag cgcgccgaca tccatgtcgc
     1921 tggtcgtccc gctgaacaga tacgaggggg tcccgttggc cacggccagg accaggtcgc
     1981 ccgagagctg ccctccggcc accgaggatc ggaggctggt gaccgtgacg gtgccggcgg
     2041 catactgcat cctcgcgtgg aggccctcga atccctgacc gttgaggcgg ccggtggccc
     2101 cggtgatctc gccgtcgaga tccagcgcct gcaccggacc ggagacctgg atctcggcgc
     2161 ctgcctgccc ggtgatggtg aggcgcgcgt gggggaagaa gagcgcctgg acggtggcga
     2221 gatcgagcgc gggggaccga agccggagct gcacccacgg ccccccggcg aagaggaggg
     2281 tgccgtcggc gctcagcggc gatccgtccg cgtgcagcag gagccccggc gtctcggcgt
     2341 gcgcgccgtc gaccgtaatg ggacccgaga cacccctgag ccacaggtgc gccgggcgat
     2401 actcgacatc ggtgtcgtgc agccggatcg ccgcggtgta gtcgagcgcg gagtccgacg
     2461 gggactcggt gagcgcgagg tggacctggg cggcgaaccg cccccgcgcc cagcccagct
     2521 cggggaggtg gacgaaatac ggaccccagc gtgcggccga cgcattcgcg gccgtgagat
     2581 cgaacgcgga ggttctccag cccggacggt atcggccgct gagccgtacc gcttctgagt
     2641 cgcgactcca cccggtgagc gcgatcgcga cctcgccccc ctggacctcg acgtcgccat
     2701 tcacccggtc gaacgtcacc gcaaacggcg atgcgggcgc gttctcggcg tcgctgaact
     2761 cgagcgtgcc gtcgtggatc aggacgcgcc cccgcaggcg ccccgccgcc ggcgggccgt
     2821 gggaacccag gaggtcgtgc acgttccacg ccccctcggc actcttggag agcgcgagat
     2881 gaggatgccg caggtcgacc tggatgatgc ccccgaccgc caccggccgg gcggcgagga
     2941 gggaccgcca gtcgaacgac aggcgcacct cctccgagga gaacgcgacg ccgtgcgcaa
     3001 acccgccccg ctcggcgatc cggagatcgg aaagcacgac gccgctcagg agggatccgc
     3061 tgacgccgcc gagcgttacc tcgcggtgca ggctcgcttc gagctgccgc accacgaccc
     3121 ggtggacgtc cgtggcgaac cggccgctcg agaggtagcg atgccagagc aggccggcaa
     3181 agacggtgat ggccaggacg gcgaatgccg tccacgccgc caccctgcgc gcccggatcc
     3241 tgcgccgggc cgcgcccggt ctcggagcag tcagcgcgcc cctcgaccga tcacgatggg
     3301 ggtctggaac ctcccggtga gcacccacgg cgtggtccac cgcgtcgaca cgtgggccgg
     3361 tccggcggca gagccggccg gcggccgcgc cccgctgacc aggtcaacga cgacatcggt
     3421 gctcttctcg ccctcttcga acgcggcgat ggcatccgcg agcgtgttga tcgcagcggg
     3481 cccttgctgc ggcgaggata acgccccggt cgccggctgg ggcgcggccg atccgccacg
     3541 cacgataagc atcgcgggcc cttcgggaaa gtctgccggc accaccacct cgatatcccg
     3601 ggtcacgggc gcctcgcgga aggggcggac ggagacccgc acccggacgg tcccgcccgg
     3661 ggagacgggt cccttgggca tctcggcctc cgtgagcgtc ccggtctggc gcgccttggt
     3721 gatgcggatg tcgatgctga tgtcggtggg gcccgcgtcg gcgaagtcgt tctcgaacag
     3781 aagacggagg gcctgcggca cctcgatgag cgcgcgggat gcgatgtcgg tgctgctgta
     3841 gaagatgttc gcccgctcaa cgcgccgggg cagcccccga ccccccaggg cgatgcgcac
     3901 ctcggcggtt ccctcgcccg atcggttcag agcccgctcg accgctcccc gcgcccccag
     3961 ggcgaccaac acgggggcca gctccttgct gttgaccacc tggaacgcga agcgccggtc
     4021 cgtgcccgcg tcgctgtcat gcaccgcgac cctggccccg aacatgcggg gcaacacccc
     4081 cagcgttccc cccaccgcgg ccggccggtc ctcggaaatg atcccgaccg gcgcgccggc
     4141 cgcgccgacc ttcaggttgt gattcatggc tcgcaccgtt tgaaaaataa tggcgttcgt
     4201 cagcaggaac tcggtccggc cgttgtccgt gaatgggtgt ccaaaggcca ggatccggtt
     4261 gccgtcgcgg tacgtgagcg tgccgatcgc gtacgcggcc aggtcgccct gcatcagcgc
     4321 caccccgatc gcgctgccgg gttcgagccg gatcgtgggc gggaggctga ccatgcccgg
     4381 acccgccatc ggcagcaacc ccatcggctg gagcgagcgg gccagctcct cggttgcttc
     4441 atacccaagt ccagagacga atagcggggt ggccgcgggg acggcggtcg ccgtgtccgg
     4501 ggccgtcgga gtcgggtcgg gagatgctcg gagctcgccc cggcggatgg tgcgcccgcc
     4561 gagggcaaac ggggtgacgg catacacgcc gggaggcggg acgcggaccg cgcgcggcat
     4621 cgcccggagc atatcttcga tcggcgtgaa cagccccacc atcggatcgg cgaactggaa
     4681 cgagtaactg aacgctcctg cgagcttccc gccgagatag atgggactgc cgctcatgcc
     4741 ggacgccagc ccgcccgtcg cctgaatcgc cgacccgaag gcccggaaga gcaccagatc
     4801 ccccgccggc ccggcgttcc ggagcacccc caggacgatc acctggaact cggagatcgt
     4861 ggtcccgagg atgaccgtct tcccgatccc ggtcatgccg gcggtaatct gcgccgacgg
     4921 cagcacggcg ggggatgcca ccagcggggg gggagcgccc agtagcccgg ctgccagccc
     4981 cgcggccagt cccgctgcac cccaacgccc cacacgcccg gccatcccca tcgtgacctc
     5041 cgttggccac gccacagggt ggcgtgcccg cgtcgcctta ctctaccacc atcgggattt
     5101 gaaatctccc caggaccacc cactccgtcg agaacgaggt gccggtcgag gggtcaaacg
     5161 ccgggttggc gttgcccccc accgcctgca gcacgggagc cgggaggagg gtcacgagca
     5221 cgtcggtgtt cttcccggcg tgttcaaact ggtccaccgc gtcgtccaac gacgcggcag
     5281 tcccctgcgg gctcccctgc aggccggcga ggagctggaa cttctggtcg gttggaatct
     5341 gcgccgcatc gttgagcccg cccgcgctcc ccacgaggag gaacgcgggt cccctgggga
     5401 agtcgcgcgg gacggtgaac tcgacggtgc gggccaccgg atcctcgttg ccgaacggga
     5461 ccagcccgat ctcgacccgc acccgctgac cgcgggtcag ggtccgcgtc ggggtgtcgg
     5521 cggccacgag cagcgcggtg ttcgggcgat tctgcacggc gatgtcgatg gtcatgtcga
     5581 tcggctcgag ggtcttgaag aagttcccga acagcaactg ggtcgccccg gggacgtcca
     5641 gcaccgccgc ggtggcgata tcggccgcat caaacgcgag atcctcgcgg acgatctcgt
     5701 gcgcgatccc gagtgcgcgg agcccgatcc gcacgcgtgc ggacccgctc gaaacgcggt
     5761 ccagggaccg ctggacggcg ctcaacacga cggtcgggac gagggactcg gccaggtcgc
     5821 cgcggcggac gacctgggcg gcggtttcgc gcagcggccc cgcgccgtcc cggacgcgca
     5881 cgcggacccc gaacatgcgg gggaaccggc ccacctctcc cgccaccccg gtgctccggt
     5941 cctgcgtcgt ggacccgatg agggggccga ggctgccctc cttgaaggga aagtccatgg
     6001 accgcaccac cgtgtcgatc cacgcggccg ccaacggcat cgtcgcccac ccggcgttga
     6061 ggaggggatg gccaaaggcc aggacctgac cgccgcgccg gtaggtcacg gtcccgatcg
     6121 cgccgacctc gatgtctccg cgcgccagtt cgatgccgat cgaactgccc ggctcgaccg
     6181 ccgggggggt gaactggcgg ctccctccgt atccctgcat cgggacgacg ttgtagcggt
     6241 tgagctggcg ggcgagcaac gcgagggccc ggggggagaa cccgcccgtg aacatcggca
     6301 cggcggtcgg ggcgaccgcg accgatcggg ggagcggatg agcattgtac gcggccgcat
     6361 ccgcggtgga ccgcatcagg accacgcggg agaccacccc gcccggcgtg gggatcggcg
     6421 ccgccgcctc gaagacccgg ggggggcggc cccaagcggt gtcggaggca ccggggggcg
     6481 ggggcgccag gagcttcaac atctcctcga tcggcgtggc caggctgagg tcggcatcct
     6541 tccctgcgaa gtggtagccg tagctcaacc cgccgatcac gcggccgtcc acatagagcg
     6601 ggctcccgct catccccgcc gcggtgccgc cggcctcctg aatcaccggg ccggtcgctc
     6661 ggaacaggat gaggtcgctc gtggggccgc ccttgaggat gcccaagacc tggatgccga
     6721 accgcgtgat ggtcgtgcct tggagcacgg tcaacccgta cccggtcatc ccgggcctga
     6781 cctgcgccag gggaagaatc ggtggggcgg ccgcagcagc cgcgcgcccg ggagacggcc
     6841 atcccccgaa gacaattgcc aggattgcta gcgctcccca tcccctccgc acgcgatggc
     6901 cctccggcac ccgtgttacc tgaaagaacc gtccataaaa aggacgaggg ggaacacaag
     6961 tccccctcga cgtcccggcg cgtccgccct cacttgggct ggatcacggc cacaacatcc
     7021 ccgatgttga cgcggtctcc tggccggacc attacctgta ccacctgacc gtccaccgac
     7081 gcgaccgctc caggtacggc cgcccccgag ctcgttccgc gaagaaacac cagcgggtcc
     7141 ccctgcctca cgacctggcc gaccttggcc agttgcagcg gaagcacctc cccggagatg
     7201 accacccgga cgaggacgtt agcctgctgg gctggaaccg gaggggacaa caccgctgtg
     7261 agcccgacga ccacgagggt catgcacagc gcccacctgg atagcgacat cgcccatcac
     7321 tcccttgcaa catcccatta taccgagggg tcggcgcagg tgtcaaacgt atgaccgccg
     7381 tgcccggcgg ctgccgctgc ggccggcgag ggccgcagcc ccccctctcc cccggatatg
     7441 tacggagaat tcacgcggga ggttccgggc aaagccgaag tagcgccgga gtccccccac
     7501 gatccgttcg gccgcgtgcc cgtccccgta ggggtttctg gccaggctca tccgcgcgta
     7561 ggcgcggggg tcggtcagga gccgggacgt ctcctggacg attcgggtgt ggtccgtccc
     7621 gaccaggcgc gcggtgcccg cggcgatccc ctccggccgc tccgtcgtct cgcgcaggac
     7681 caggacgggc cggccgagcg atggcgcctc ctcctggatc cctcccgagt cggtcaggat
     7741 gaccgtggcg gccttcatgc acgccacgtt ggtggcgtag tccagcggct ccgagagatg
     7801 cgcccgcgga tgcccaccga gaatctcccg cgccggccgg ctcacgaccg ggttggggtg
     7861 aacgggaaac acgaggtcga gatccggaaa gcggtccagg atctcgcgga ccgcgaggaa
     7921 gatgcgacgc tgcggctccc cccaattctc ccgccggtgc gtcgtgagca gcagcacccg
     7981 gcggccgcgg ccggcgagca ccccccgcag gcgcggatcg gccggcggcc ggtcccgccg
     8041 gacgacctcc aggagcgcgt cgatgaccgt gttcccggtg accaggatcc gatcacccgg
     8101 caccccttcc gctttgagat tctcgcgggc ggcggaggtc ggcgcaaagt ggagatcggc
     8161 cagcaccgtg gtcatccgcc ggtacatttc ctcggggaag ggctggtact tgtccgcggt
     8221 ccgcaacccc gcctcgacat gccccaccgg aatccggcga tagaacgccg ccagggaccc
     8281 cacgaagctc ggcgcggcgt cgccctggac gagcacgaga tcgggcgcga ctttcgggag
     8341 gagcgccgag agcccctcga gcgtccggac ggtgatgtcg accagcgact gttgctccgt
     8401 catgatcccc aagtcgtggt ccggggtgat cgcgaacagc gacagcacct ggtccagcat
     8461 ctcccgatgc tgcgcggtga ccgcgacgat cggggtgaac tcgaccggat gggtgcgcag
     8521 ggcgcggacc accggcgcca tcttgaccgc atcgggccgc gtcccgaaga cgaccatgac
     8581 cctgcggggg ggggcggcgg tcacctgggc acctcggtcc ggccgcgggc ggacggtgac
     8641 gggggggtca gcaaccccgt gcggcgggcc ccgaaggcca gcgcggccag gagcgcgccg
     8701 aggacggcga cgagggcaaa ccggtggacc ccgctcgcga gcagcgcccc ggcgcccagg
     8761 acgccggtca ccaaatacag cacgacggcg gtctgccgct ggctcaaccc gcgctggagc
     8821 aaccggtggt gcaggtgctc cgtgtccgcc tggaagatcg ggcggcccgt ccgccaccgg
     8881 cgggcgatgg cgagcgcggt gtcgaacacc ggcacgccga gcgcggcgag cggaacgagg
     8941 agcgagaggg ccgtgtaact cttataggtg cccagcaccg aaaggctgcc gaggaggtag
     9001 ccgagcagca tcgatccgga atcgcccagg aagatgcgcg cggggttgaa gttgtagggc
     9061 aaaaagccca gggtgctccc cacgaaggcc gccgccagca tggcggtgcc cgcgtccccc
     9121 cgctggtagg aggcgatcag cacggtgccg ccggcgatcg cggcgatgcc ggtcgccagc
     9181 ccatcgatcc cgtcgatcag gttcatcacg ttgcatagcg ccacgatcca ggcgacggtc
     9241 agggcgaggc caaaggggcc gaggaagagc atcccgccca gcgggttcgt caagacatcc
     9301 atcccgaccc cgaacgacag cggcacgatc gcggccacga gctggccgaa cagcttctcc
     9361 atcggcgaga cgccccagat atcgtcgatg atgccgagga tcgtgatcag cgtcgcgccg
     9421 cacagcaccc cgatgaccgg gcgatcgaac tggggggtga agggcacggt gagcaccacg
     9481 tggtgcgctt ccagcgtgat cctgacggag cgctccagcg gcagcccggc gagcaccgcg
     9541 acgagaaacg cgagatacac cccgatcccc cccaggcgtg ggaccggcac gcggtggata
     9601 tgccggcccc cgggctgcgc cagcgctccg acccggatcg ccgcccattc cacgaggggg
     9661 gtgagcgcat acgcgaccac gaggccgatc gcgccggcca ccaggaaccg agagatcgtc
     9721 atcgcggagg cgcctggagg ggcgtcactc cttttcggcg accggttgca tgacccgagg
     9781 ctgcgcgaac agttcgctca ccgactggtc ggcgtggatc cgccggatcg cctccgccag
     9841 cagccccgcg accgagagga cgaccgtctt cgcggtccgc ttctcctggg gcaccggaat
     9901 gctgtccgtc accaccaatt ggcggatcgg cgaccggagg atgcgggtcg tcgcgggggg
     9961 cgacaggatc gcatgcgtgc agcaggcgta gacctcgtgg acgccgcggc gcacgagggc
    10021 ctcggctccc atcaccagcg tcccgccggt atcgatgatg tcgtccacca ggatcgccgt
    10081 ccgccggtac actttgccga tcacatggac cacgtcggcg acctggttgg ggcggtcgcg
    10141 ccgcttgtcg atgatcgcca gcggggcctc gaggtactcg gcgaactccc gggcccgttt
    10201 gacgccgccg atgtcggggg agaccaccac gaggttctcg agcccgaggc cgcggaagta
    10261 gtcgcccagg atcatgcggc agggcaggtg gtcgagcgga atgtcgaaga acccccacat
    10321 ctgtcccgca tggaggtcga gcgtcaggac gcggtgcgcg cccgcggtgg tgagcaggtt
    10381 ggccaccagt ttggccgaga tcggctcgcg gggcttgatc ttgcggtcct gccgggcata
    10441 gccgaagtac gggatcaccg cggtgatgcg agaggcgctg gcgcgccgga cggcgtcggt
    10501 gatcacgagg agctccatca ggttctcgct cacggggggg caggtcggct ggacgaggaa
    10561 cacgtcctcg ccgcgcacgc tctcctcgat ccgaaccccg atctccccgt cggcgtaccg
    10621 gaagacgttg atcgccccca gggggagatc gagggcggcg gcgatgccgc gggcaagatc
    10681 ggggttgccc gttccgctga agacccggag ccccgcgccg gtcatcgctt tgccccgagg
    10741 acgcggatca cgcgcgccgg caccccgacc acgacgctgc ggggcggaac gtccttcgtc
    10801 accaccgcgc cggcgccggt gatcgctccc cgcccgatcc ggaccggcgc gaccagcatc
    10861 gagtcgctcc cgatatacgc cccgctcccg atggtggtgc ggtgtttccg gcgatcaagc
    10921 ccgtagttgc acgtgatcgt tccggccccg atgttgacat cggtgccgat ccaggcatcg
    10981 ccgaggtagc acttgtggtg gaccttcgtc cggtcgccga cgcgcacctg tttcatctcg
    11041 gcgtagttgc cgatctcgac atcccgcccg accaccgtgc ccgggcgcag gtgcgcgtag
    11101 ggtcccacga ccgatccatc gcccacgcgc gcgccctcga gcgaggaatc acggatccgc
    11161 gcgcggcggc cgatcaccga atcgtggatc cgggcgccgg gcccgatcgc gcacgcccgg
    11221 cccaccaccg tcctcccggt gacgaacgtc aacgggtgca cgaccgtgtc ctgggcgatc
    11281 cggaccgtct cgtcgatgaa ggtcgtcgcc ggatcgatca cggtgacccc cgactccatc
    11341 acgcgggtca ggatccgccg ccgcatgcaa cgttcggcct gggacaactc ttcgcggctg
    11401 ttgatgccga tcatctcgtc gggatcgggc acatcgacgg tcgcgacgcc gccgtcctcc
    11461 tcgcccagga gattgacggc gtcggtcagg tagtactccc gctggtgatt gttgggacga
    11521 acgcgttcga gggcccggcg caactcgggg acgcgaaagc agtagacgcc gacgttgacc
    11581 tcctggacgg cgcgctcccc gggggtggcg tctcgatctt ccacgatgcg ttggaagcgg
    11641 cgctggccgt cccggaggat ccggccgaag tggtgggcgc cggccaggac cccggtggcc
    11701 accgcggccc gggcgccgga cgcccgcagc gcctcccgca acgcgcgtaa cgtggtggga
    11761 gagacaaacg gcatgtcccc gtagaccacg tagaccaggc gctgtcgtgc gaggcggggc
    11821 agcgcctgcg cgaccgcgtg gccggttccg agggggcggc gctggacgac atacgggagc
    11881 gtggtcccga tcaccgggcg gaacgccgcc gcatctttcc cgacgaccag cagcggtcga
    11941 gccacccccg accgccgcac cgtttccacc gcgtacaaca ccatcggtcg gccgcacagc
    12001 ggatgcagca ccttgggaag atccgatttc atccgcgtgc cccgccctgc agccaggatg
    12061 acggctgcca gctgtgtcac cacgctacct ccaacgccgt cctcaaggga ttcgagggga
    12121 gggaacccgg cccctgcggc agaggcccgc gcgggcccgg gcggagaagg ccggggtgtc
    12181 cgtacaacgc gaggagcccg cgcagcgcgc ggctcctcag cgtcgcatcg gcgtgctcgg
    12241 cgtgctcacc gcggccgctg ccctgccttt tcccgctccc gctcccgttc gatgacccga
    12301 cagaacgctt ccaccacccg cgggtcgaac tgctgccccg cggcctgatg cagttcggcc
    12361 acggcctccg catggtcccg cgccttctga tagggccgct cgtccgtgat cgcgctgtag
    12421 gcgtcgcaga ccgcgaggat gcgcgcgccc aagggaatct cctcgccctt gagcccatcg
    12481 ggatatccgg tcccgtccca cttctcctga tggtgccgga cgagggcggc cacgccccgc
    12541 atccgtccga tgggggtcag gatctcctcc ccgatgaccg ggtgccggcg catgatcccc
    12601 cattcctgcc cggtcagatc gccgggcttt cgcaggatcg cgtcggggac gccgatcttg
    12661 ccgatgtcgt gcaacagcgc cccccagcgg acgtcctgga cctccccttc cccgcacccg
    12721 agatcccggg cgaccgcctc cgcccactcc gagatgcgct cgctgtggcc ggacgtatac
    12781 gtatcgcgcg cgtccatcgt ccgggccagg ctgatcacca tctgcatgta ggattgctcg
    12841 aggttttcaa acagccgggc ccggcggatc gcgttgcccc cgatctcggc gatcccctcc
    12901 agcaggcggg cctccgcctc ggcaaacggg ctggcgtcgg agcgccgctt ccggccaagg
    12961 cagaacaccc cgatgatttc ctgctcggat cgcaccggca cgatcacggc cggcccgagg
    13021 gcgcggaaac tgctcagcca gacgggcagc gggtccccgc cgaaattcgg ggtctgaaac
    13081 gtcgtgccgg tcttcaccac gcgctcgaac gggctgcccg gcagcgggaa ggtcgagccg
    13141 cgcacttcgg gcggcagccc ggccgcgaac acgcactcga actccgcgcg ctcgccggcc
    13201 aggagcgtga gcaccccgct gtctgcgtgc atcagatcca tcgcccgtcc gaccaggatc
    13261 gggtagatct ctcccgtgtt gcgagccgcg cgcaacagcc gggtcagctc gaacagcacc
    13321 tccagatcgg ccgtgcgctt catctgggcc tcgcgcgccc cggcgttgtg gagggcaaac
    13381 gccgcctcct cgcaaaaccc ctggaacacc ttttgctcgg gcgccgacca ggtccgcggc
    13441 gcgtcgtagt agcacccgat cagcccgagg acggccccct cgtgcacgag cggccagctg
    13501 gccagcgcgc gatacccctc ggcttgtgtc aggcgcggaa cggcgaccgc cggggacagc
    13561 gcgagaacat ccggatacag gaccggcgcc gagccgtcga tcccccgccc ggagatctcc
    13621 agcagatccg gtttggcacc gtccatcacg cggccgccca cgagggtttt tgcgtggcgc
    13681 agcacctggt ccacgtaggc gtgggagagc cccaccgacc aggggcacgc cagcgtccca
    13741 tcgggctggc agaggtacac ggccgtgcgg tccgcgctgc ccaggacgcg cgcgccatcc
    13801 ccgatggtct ccgcgacctc ggcgacagtc gcacgacggt gcagggtcct ccccgtggtg
    13861 cgaaaccggt cgaacagccg caggcgctgc tgggtttcct cgaagaggcg cagccggggg
    13921 gccgccgccg ccagaacctc cccgaccgat tcgaccgccg cgaggtcgtc gggcgtccag
    13981 ggccggggcg cggccgaggc caccaccagc cctccggcca ggccgccctt ggtcccgaac
    14041 gacgtgatga tcgacgcccg gatcccccgg gccatgaact gctcggcgat ccccttaggg
    14101 gcatctgcgg gcgccgccgc gcgccagtcg gcgacgcaga gcgctttgcg ggcccccccg
    14161 gcatcggggg gggcgatctg cgcgagggcg gttcccaggt cggacgcaat cccccgggcg
    14221 acggacacct ggccgatcca cgcggccccc atcatgacat cgagcgcgcc gagcgtgcgg
    14281 tcgatcgtct cttcgaacag accctggagg tcggtcgcgc ggagcgcgga ggcgacaatg
    14341 gcctgcaggg tctccggatg cgcgccgcgg cgtccgccag cgtcctcgac gcgcgcgcgt
    14401 ttcgccacct cctcgatggc gatgacaaac cctccccacg actgcgtcgg gccgttgagc
    14461 ggggccgccc acacccggac ctcgagcgca gcgccgttct tccggtggga actcacgcgc
    14521 gtcccattcc agggcccgct ctcgcggagc gcctgcgcga gcgtccgcgg accggccccg
    14581 ccgtcctcga gcacgaaggg gaacggtcgg gcgagcacct catcggcgct ccagccgagg
    14641 atccgttcgg ccgccgggct ccacatctgc acgtttcctt tgggatcgat ggcgatgat
//
LOCUS       DASQGH010000148         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28990, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000148 DASQGH010000000
VERSION     DASQGH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28990"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VEW91_02780"
     CDS             <1..496
                     /locus_tag="VEW91_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90545.1"
                     /translation="RARPRVVCLEWLAPPFCGGHWMPEVVRLAGGRDLIGRPGRPSFR
                     IEWAQIAAARPDVVVLVLCGFDLARTQREAEGLAARTEWQDLPAVRAGRVYATDASSY
                     FSRSGPRIVDGLEILAALLHPGAVSWPTPPNSWAPLRAAGPPGMRPNGRDARDLLDQG
                     GRVR"
     gene            493..1275
                     /locus_tag="VEW91_02785"
     CDS             493..1275
                     /locus_tag="VEW91_02785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM90546.1"
                     /translation="MTAYLEGKVAIVTGGGRGIGRAIAERLARDGAAVVVADRDEDTA
                     RDVAHTLASEGRRSLAVKADVGTAEGRRRFIDAAVETFRRLDILVNNAGIVQAHRPEA
                     VTEADWDAIMNVNCKAVFFACVAARPHLARAGGGRIVNLASIAGKIATPWWAPYGVSK
                     AGVIALTRSLAVAFAREGIHVNCVCPSPVDTAMWHQIDVDEAHKIGFAPGELTKTRLG
                     SIPLGRIAQPGDVAGVVAFLCSPDSGYMTGQAINVSGGMVSH"
     gene            complement(1313..1840)
                     /locus_tag="VEW91_02790"
     CDS             complement(1313..1840)
                     /locus_tag="VEW91_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90547.1"
                     /translation="MGTQTDRKALRATAAAVAIAAAMGLGGGAAATAAGFPDVPPWHW
                     AYAAVTRDQDAGVIIGYPTSSAALIENTIVQVFDGFAHASTSGAQAWVERFSYNLPPS
                     WPAPLRRSQIVTFSLTGMRISVNGDTAAAAFTAALGIRGGQSVTVSMRVVLRSNGQDW
                     QVNYVTLARASPLFM"
     gene            complement(1861..>2948)
                     /locus_tag="VEW91_02795"
     CDS             complement(1861..>2948)
                     /locus_tag="VEW91_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HYM90548.1"
                     /translation="IDRTELVSRRMADLKLGQAMKKSHRFKVWMQDGASSLRHLLHSS
                     LAFTRVVSIERVAYPQKFVYCFEVAGDPPAFFVEGGILTHNCFGYQGYKNARFGQIEA
                     HECTTALGREMLLRAKDVAESQGYTMLHALVDSVWLTRPGAARADYEALAQAIVRATG
                     LPIAVEGVYRWIAFVPSRTHPLVGVPNRYFGVFEDGTEKVRGIELRRGDTPPIVATMQ
                     RRMLDVLFEAGTLDEARARFPVLKELLDEQIIRLRQHEVRAEELVIRTVLSQDPREYE
                     RGIPQAVAAHQLVQAGVTLHPGEPIEYIITNAAAKIPAERAAAYARLPSDWCYDVDRY
                     TLMLRRAVDTLLAPFRNGYHGNGTGSG"
BASE COUNT          454 a         1004 c         1025 g          465 t
ORIGIN      
        1 gcgggcccgt ccccgggtcg tgtgcctgga atggctcgct cccccgttct gcggtggaca
       61 ctggatgccc gaggtcgtga ggctcgcggg gggccgcgac ctcatcggcc gccccggccg
      121 gccgtcgttt cgaatcgagt gggcacagat cgccgcggcg cggcccgacg tcgtggtgct
      181 cgtcctgtgc gggttcgacc tcgcgcggac gcagcgcgaa gccgaagggc tggcagcccg
      241 caccgagtgg caggacctcc ccgccgtccg ggcgggccgc gtctacgcga ccgacgccag
      301 cagctacttc agccgctccg gtccccggat cgtggatggg ctcgagatcc tggccgcgct
      361 attgcatccc ggcgccgtat cctggccgac cccacccaac tcatgggccc cgctgcgcgc
      421 tgccggcccc cccgggatgc gaccgaacgg tagggatgcg cgtgatctct tggaccaagg
      481 aggcagggtg agatgacagc gtacctcgag ggcaaggtcg cgatcgtcac cgggggcggt
      541 cgcgggatcg gccgggcgat cgccgagcgg ctggcgcgcg acggcgcggc cgtggtggtg
      601 gccgaccggg acgaggacac ggcccgggac gtcgcgcaca cccttgcctc ggaggggcgg
      661 cgcagcctgg cggtgaaggc ggatgtgggg accgccgagg gacgccgccg gttcatcgac
      721 gcggcggtcg agacgtttcg ccggctggac atcctcgtca ataacgccgg gatcgtccag
      781 gcccaccgcc ccgaggccgt gacggaggcg gactgggacg cgatcatgaa cgtcaactgc
      841 aaagccgtgt tcttcgcctg cgtcgccgcc cggccgcacc tcgctcgggc cgggggcggg
      901 cgcatcgtca acctcgcctc tatcgcgggg aagatcgcta ccccgtggtg ggcgccgtac
      961 ggggtcagca aggccggcgt gatcgcgctg acccggagcc tcgcggtcgc cttcgcgcgg
     1021 gaggggattc acgtcaactg cgtctgcccc tcgccggtcg ataccgcgat gtggcaccag
     1081 atcgacgtcg acgaggccca caagatcggg tttgctccgg gggagctcac gaagacgcgg
     1141 ctggggagca tcccgctcgg ccggatcgcg cagccgggcg atgtggccgg cgtcgtggcg
     1201 ttcctctgct cccccgactc agggtacatg accggtcagg cgatcaatgt gagcgggggc
     1261 atggtttcgc actgagcgag ggccgggtcg gagccacgcc gaccctgacg gtctacatga
     1321 acagcggact ggcccgggcc agcgtcacgt aattcacctg ccagtcctgc ccgttggacc
     1381 gcaacacgac gcgcatcgag acggtgaccg attggccgcc tcggatcccc aaggccgcgg
     1441 tgaaggccgc cgcggccgtg tcgccgttga ccgagatgcg catcccggtg agggagaacg
     1501 tgacgatctg cgaccgccgg agcggggcgg gccagctcgg cgggaggttg tagctgaatc
     1561 gttccaccca cgcctgggcg ccgctcgtgg atgcatgggc gaatccatcg aagacctgga
     1621 cgattgtgtt ctcgatcagt gcggcggatg aggtcggata accgatgatc accccggcgt
     1681 cctgatctct ggtcaccgcg gcatatgccc aatgccaggg cgggacgtcg ggaaaccccg
     1741 cggcggttgc tgccgctcca ccgcccaatc ccatcgcagc tgcgatggcc accgctgccg
     1801 cggtagccct gagcgccttt cggtcggttt gcgtccccat gccaggcctc cgttccacgg
     1861 tcatcccgag cccgtcccgt ttccatgata gccgttccga aagggcgcga gaagggtgtc
     1921 cacggccctg cggagcatca gcgtgtaccg gtccacatcg tagcaccagt ccgagggaag
     1981 gcgggcgtag gcggccgcac gctccgcggg gatcttggcg gcggcgtttg tgatgatgta
     2041 ttcaatcggc tcccccgggt gcagcgtgac cccggcctgg accagctggt gggcggccac
     2101 ggcctggggg atcccccgct cgtactcccg gggatcctgg gagagcacgg tccggatcac
     2161 cagctcctcc gcgcggacct cgtgctggcg cagccggatg atctgctcgt ccagaagctc
     2221 tttgagcacc gggaagcgcg cgcgcgcctc gtcgagggtc cccgcctcga acaggacgtc
     2281 cagcatccgg cgctgcatcg tcgcgacgat cggtggggta tccccccgcc gcaactcgat
     2341 gccccgcacc ttctccgtgc cgtcctcgaa caccccgaag tagcggttgg ggacgccgac
     2401 gagcgggtgg gtgcgggacg gcacaaatgc gatccaccgg tacacgccct cgaccgcgat
     2461 ggggaggccc gtcgcccgaa cgatcgcctg ggcgagcgct tcgtagtccg cgcgcgcggc
     2521 cccgggccgc gtgagccaga ccgagtcgac gagagcatgc agcatcgtgt acccctgcga
     2581 ttccgccaca tccttcgcgc gcaggagcat ctcccggccg agcgcggtcg tgcactcgtg
     2641 ggcctcgatc tggccgaagc gggcgttctt gtagccttga tagccaaaac agttatgggt
     2701 caggatgcct ccctctacga aaaaggccgg gggatcgcct gcgacctcaa agcagtagac
     2761 gaacttttgt ggataggcga ccctctcgat agacacgacc cgcgtaaacg cgagggagct
     2821 atgaagcagg tgcctcagag atgaggcacc gtcctgcatc cagaccttga atcggtgaga
     2881 tttcttcatg gcctgtccaa gcttcaggtc tgccatcctc cgtgaaacca gctccgtcct
     2941 gtcgatac
//
LOCUS       DASQGH010000149         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51568, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000149 DASQGH010000000
VERSION     DASQGH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51568"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VEW91_02800"
     CDS             complement(<1..453)
                     /locus_tag="VEW91_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM90549.1"
                     /translation="MTGREVRFGVALKNFTAYPDEPSIDDIIAFATRAEALGFESAWV
                     WDHILLGSKRPFPYLESLATLTALAMRTQRLRLGTGVLVLPLRNPVVLAKVLTSLDHI
                     SKGRLILGLAAGWYEREFEACGVPFKERGKIFVRNLEVLKRFWTEDQVN"
     gene            complement(450..2801)
                     /locus_tag="VEW91_02805"
     CDS             complement(450..2801)
                     /locus_tag="VEW91_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM90550.1"
                     /translation="MTRARGHPSDAVPPSPYRVIGRPLPRFDVTDKVQGTTLYAADWR
                     LPGMLVGRILRSIYPAARIRRLAVERARTLPGVAAVLTAEDVPANMLHEDPTGLGLVS
                     FATPVLAPGRVRYQGEPIALVAADSEVHAQAALEAIEVEYESLPGVFDVERALRADAP
                     RVHEDRGNLLIHWKLRQGDVEEGFRRADVIVERTYRTQRVDHAYLEPEAGVAWVDGDG
                     VVNIRASTQVIEHFREIAEILHLPYNKVRVIAPFLGGGFGGKEDMTVEPYVALLAWKT
                     GCPVRMVWSRQESLLARPKRHPFAMHYKTGATTTGHLVAQEITLLADAGPYPLLSPRV
                     LFAALVVGSGPYRVPHVKIDAMAAFTNNVPTSAMRGFGAMQVTFAYESQMDLLAAQVG
                     VSPLEIRSRNFLQKGDRLPTGETLETHVALPSLTPLAVDALGERSRPSRPSARVGRGL
                     ACNMQPYGRTVWFRDRATAWIGFETDGSLAIRTGVTDVGGGQAASLAQIAAEVLGVSP
                     DRIAVHIGDSALTPLAGGTFATRQLYMSGNAVLKTARELGVMMIPVAASLLEAAEADV
                     QFADNHASVIGSPERRLALARVVAECARRGVPTAHLSAFQGEQAPPVDLETGQGKTFP
                     DYTFGCHAVEVEVDTETGVVTLLKHVACHDVGRAINPQSVEGQIQGGAVMGAGQALLE
                     EVTLEEGNNLTTLFANYLIPTSLDVPDVRAIALESAEGKGPFNARGIGEPPTGPPPAA
                     IAGAVCDAVGIRPLDLPFAPERLFDALEARRTGPGGNRREVAG"
     gene            complement(2798..3298)
                     /locus_tag="VEW91_02810"
     CDS             complement(2798..3298)
                     /locus_tag="VEW91_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYM90551.1"
                     /translation="METASVDASERLGLHFVLNGRPVDTWVGPHETLLAVLRDTLGAT
                     EVKYGCGEGVCGTCTILLDGAPINACLILAVQVRGRAVTTVKGLGPEGDLHALQRAFV
                     AREAAQCGFCTHGMLLVAYAFVRDHPRPTRDEIRAALAGNLCRCTGYTRIVDAIEAYV
                     RQEGPA"
     gene            complement(3310..4194)
                     /locus_tag="VEW91_02815"
     CDS             complement(3310..4194)
                     /locus_tag="VEW91_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYM90552.1"
                     /translation="MYPFPFAYHAPANLEEALTLLARLPDGKVLAGGQSLMPLMNLGL
                     ARPGALVDVNGLPGLDGVTEDHGHLKLGALVRHHVLETSPLIRRRCPLLAEAASLIGN
                     IRVRHRGTLGGSLAHADPAAELPLVAVAVGATLEIRRAEGVREVAAEQFFRGYLLSDL
                     EPGELVTGIRIPAFGPATGYGLAELVRRAGDFAIVAACALVDGDGAGRCRQASLVLGG
                     VGPHPLRMRRAEALLGDRALTDPLLEEAAEAASAEVAPESDIHASAPYRRAMARVMAR
                     RALVMARMRAGKSRDRSA"
     gene            complement(4219..>4446)
                     /locus_tag="VEW91_02820"
     CDS             complement(4219..>4446)
                     /locus_tag="VEW91_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine (thiol)-lyase"
                     /protein_id="HYM90553.1"
                     /translation="SSGCNVVACLKVVRAYPALRAIVTMINDNGLRYLSTELGGEPRS
                     DQPPPPTPARERAPRAQDARRLSGKRLVVID"
BASE COUNT          701 a         1572 c         1528 g          645 t
ORIGIN      
        1 gttcacctgg tcctcggtcc agaatcgctt caggacctcc aggttccgaa cgaagatctt
       61 gccgcgttcc ttgaatggga ccccgcaggc ttcgaactcc cgctcgtacc agccggcggc
      121 gagccccagg atcagccgcc ctttggagat gtgatccagg cttgtcagga ccttcgccag
      181 gaccacgggg ttgcgcaggg gcagcacgag caccccggtg cccaaccgga ggcgctgcgt
      241 ccgcatggcg agcgcggtga gggtggcaag cgattcgagg taggggaagg gccgcttcga
      301 cccgagcaag atgtggtccc atacccatgc cgactcgaag ccgagggcct ccgcccgcgt
      361 cgcgaaggcg atgatatcat cgatgctcgg ctcgtccgga taggcggtaa agttcttgag
      421 cgcaaccccg aatcgaacct cgcgtcccgt catccagcca cctcccgcct gttgcctccg
      481 ggcccggtcc gccgggcctc gagcgcatca aacagccgtt caggggcgaa gggaaggtcc
      541 agcggccgga taccgaccgc atcacagacc gcgcccgcga tcgccgccgg cggcgggccg
      601 gtgggtggct cgccgattcc cctcgcgttg aacggcccct ttccctcggc ggactccagc
      661 gcgatcgccc ggacgtcggg gacgtccagg gacgtgggga tcaggtagtt ggcaaacagc
      721 gtggtgaggt tgttgccttc ctcgagcgtg acctcctcca ggagcgcttg accggccccc
      781 atcaccgcgc cgccttggat ctggccctcg acgctctgcg ggttgatcgc gcgtcccacg
      841 tcgtggcacg cgacgtgctt gaggagggtc acgacccccg tctccgtgtc tacctcgacc
      901 tcgacggcgt ggcacccgaa cgtgtagtcc ggaaaggtct tcccctgtcc cgtttcgaga
      961 tcgacgggcg gcgcctgctc cccctggaac gccgacaggt gcgccgtggg aacgccccgg
     1021 cgcgcgcact ccgccacgac gcgggccagc gcgagcctgc gctcggggct cccgatcacg
     1081 ctggcgtggt tgtcggcgaa ctggacgtcc gcctcggcgg cttccagcaa tgaggccgcg
     1141 acggggatca tcatcacccc aagctcccgg gccgtcttca atacggcgtt gcccgacatg
     1201 tagagctgcc gggtcgcaaa cgtgccgccg gcgagcgggg tcagggcact gtcgccaata
     1261 tgcacggcga tgcggtcggg ggaaacgccg aggacctccg ccgcgatctg cgccagcgat
     1321 gcagcctggc cgccccccac gtcggtcacc ccggtgcgaa tggcgagcga gccgtcggtc
     1381 tcgaacccga tccacgcggt cgcgcgatcc cggaaccaga ccgtgcggcc gtagggctgc
     1441 atgttgcagg ccaggccccg ccccacccgg gcggacggcc tcgacggtcg gctccgctcc
     1501 cccagggcat ccacggcaag cggggtgagg ctcgggagcg cgacatgggt ctcgagtgtc
     1561 tcgcccgtgg ggaggcggtc gcctttctgg aggaagttgc gggaccggat ctcgagaggg
     1621 gacacgccca cctgcgcggc gagcagatcc atctgcgact cgtaggcaaa ggtgacctgc
     1681 atcgctccga acccgcgcat ggcgctcgtg ggcacgttgt tggtgaacgc cgccatcgca
     1741 tcgattttca cgtgggggac gcgatacgga ccgctgccca cgaccagggc cgcaaacagc
     1801 acgcgggggg agaggagcgg ataggggccg gcgtcggcca gcagggtgat ctcctgcgcc
     1861 acgaggtggc cggtggtcgt cgcgccggtc ttgtagtgca tggcgaaggg gtggcgcttc
     1921 gggcgggcga gcagggattc ctgacggctc cacaccatgc gcaccgggca ccccgtcttc
     1981 caggcgagga gcgcgacgta gggctccacc gtcatgtctt ccttgccgcc aaaccccccg
     2041 ccgaggaacg gcgcgatcac ccgcaccttg ttgtacggca ggtgcaggat ctccgcgatc
     2101 tcgcggaagt gttcgatgac ctgggtcgag gcgcggatgt tgacgacccc gtccccatcc
     2161 acccaggcca cccccgcctc gggctccagg taggcgtggt cgacccgctg ggtcctgtag
     2221 gtgcgctcga caatgacgtc cgcgcggcgg aacccttcct cgacgtcccc ctgccgcagc
     2281 ttccagtgga tgaggagatt cccccgatcc tcatggacgc gcggcgcgtc ggcccggagc
     2341 gcccgttcca catcgaacac gcccggaagc gattcgtact ccacttcgat cgcctccaag
     2401 gcggcctgcg catggacctc cgagtcggcg gccacaagcg cgatgggctc accctggtac
     2461 cgcacgcgtc cgggcgcgag gaccggggtg gcgaaggaga ccaggccaag gccggtcggg
     2521 tcctcgtgca gcatgtttgc gggaacgtct tccgcggtca gcacggccgc cacgcccggg
     2581 agggtgcgcg cccgctccac cgcgaggcgg cggatccgcg cggccggata gatggatcgg
     2641 aggatgcgcc cgacgagcat ccccggcagg cgccagtcgg cggcgtagag cgtggtcccc
     2701 tggaccttgt cggtgacgtc aaaccgcgga agcggccgtc cgatgacccg gtacggggag
     2761 ggaggcacgg catcgctggg atggccccgc gccctcgtca cgccggtccc tcctgtcgga
     2821 cgtaggcttc gatcgcgtcg acgatgcgcg tataacccgt gcaccggcac agattgcccg
     2881 caagcgcggc gcggatctcg tctcgggtcg gacgggggtg gtcacggacg aaggcgtacg
     2941 ccacgagcag catcccgtgg gtgcagaagc cacactgtgc cgcctcgcgc gccacgaagg
     3001 cgcgctgcaa agcatggaga tccccctcgg ggcccaaccc cttgaccgtc gtcaccgcgc
     3061 ggccgcgcac ctgaaccgcc aggatcaggc acgcgttgat gggcgccccg tccagaagga
     3121 tcgtgcaggt cccgcacacg ccctcgccac acccgtactt cacctcggtc gcgcccaggg
     3181 tgtcccggag aacggcgagc aatgtctcat gaggtccgac ccacgtgtcg accgggcggc
     3241 cgttgaggac aaagtgaagc cccaggcgtt cactcgcatc gacagacgcg gtctccatcg
     3301 cctcccgcct cacgcggacc ggtctcgact cttcccggcc cgcatcctcg ccatcacgag
     3361 cgcacggcgc gccatcaccc gggccatcgc ccgccggtat ggggctgagg catggatgtc
     3421 ggattccgga gcgacctcag ccgacgcggc ctcggcagcc tcttcaagca gggggtcggt
     3481 gagggcgcgg tcgcccagca gtgcctccgc ccgccgcatc cggagcgggt gcgggccgac
     3541 cccgccgagc accaacgacg cctgccggca tcggcccgcg ccatcgccat ccacgagggc
     3601 gcaggcggcc acaatcgcga aatcacccgc gcggcgcacc agttccgcca acccgtatcc
     3661 cgtcgcgggt ccgaacgccg ggatcctgat gcccgtgacc aattctcccg gctcgaggtc
     3721 cgagaggagg tatccccgga agaactgctc ggcggccacc tcgcgcacgc cctctgcgcg
     3781 gcggatctcc agggttgcgc ccaccgccac ggcgaccagg ggcaactccg ccgcgggatc
     3841 ggcgtgggcc aggctgcccc cgagcgtgcc gcggtggcgc acccggatat taccgatcag
     3901 cgacgcggcc tcggcgagga gggggcagcg ccggcgaatt agcggggacg tctcgagaac
     3961 gtggtgccgg accagtgcgc cgagcttcag gtgtccgtga tcctcggtca caccgtcgag
     4021 accgggaagg ccgttcacgt cgacgagcgc gcccggccgg gcaaggccaa ggttcatcag
     4081 gggcatcagg ctctgccccc ccgcaaggac cttcccatct ggaagccgag ccaggagcgt
     4141 cagcgcctcc tccagattcg cgggcgcatg gtaggcaaag gggaaagggt acacgacgcg
     4201 gcccttggag cgtcgggatc agtcgatgac cacgaggcgc ttaccggaaa gcctgcgcgc
     4261 gtcctgagcc ctgggtgcgc gctcccgcgc cggcgtcggg gggggcggct gatcgctacg
     4321 gggttctccg ccgagctcgg tggagaggta gcgcaggccg ttatcattga tcatcgtgac
     4381 gatcgcgcgt agcgcggggt acgcgcggac caccttgagg caggcaacca cgttgcagcc
     4441 cgagga
//
LOCUS       DASQGH010000150         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_26096, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000150 DASQGH010000000
VERSION     DASQGH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26096"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1425
                     /gene="radA"
                     /locus_tag="VEW91_02825"
                     /pseudo
     CDS             31..1425
                     /gene="radA"
                     /locus_tag="VEW91_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496696.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
BASE COUNT          215 a          489 c          618 g          243 t
ORIGIN      
        1 ccattcggga agcggtgcgg ggagcggcgg atgacgcgaa gcaggggtgg ggggagcggc
       61 ccggggcggg tcgtgtatgt ctgtcaggag tgcggttatg agtccgccaa gtggctgggc
      121 cggtgtccgg cgtgcgaggc gtggaacacg ctggtggagg agcgcgccga gccgatctcg
      181 gcgagcgccg cgggcggggg gcgcacgcga ccgtcggggg gggctcccac gcccatcgcg
      241 gacgtggcgc tggatccgga accgcgggtc gccgtgggga tcggcgaggt ggaccgcgtg
      301 ctgggcggtg gcctggttcc gggctcgctc gtcctcctct cgggcgatcc cggcatcggg
      361 aaatcgaccc tggtcctgca ggtcgcccag cgcgttgccg ccgcgcgacc ggtcctgtac
      421 gtctccggcg aagagtccgt ccgccagacc aagatgcggg ccgcgagact gggggtggat
      481 tcgcccggcc tcctggtgct ggcggaaacc gacctcgagg agatcaaggc gcaggtcgag
      541 cgggtccgcc ccggcctcct catcgtcgac tccatccaaa cggtctctcg ccccgacctc
      601 ccggcggctc ccggcaccgt gggccagatc cgcgagtgca ccggcgaact gttgcgggtc
      661 gccaagtcgg acgacggccc cgccgtcctg gtggtggggc acgtgacgaa ggaaggggcg
      721 atcgcaggtc cgcgagtcct cgagcacatg gtcgacacgg tcctctactt cgagggggag
      781 cgccaccacg cgtaccggct gctcagagtc accaagaatc gcttcggctc caccaacgaa
      841 atcggcgtct tcgagatgag agggcggggg ctcgtcgagg tgtcggatcc gtcggcgctg
      901 ttcctctccg agcgccccac ggacgcgccg ggatcggtgg tcgtgtgcgc ggtggagggg
      961 tcgcgcccgc tgttgctcga ggtgcaggcc ctcgtcaccc ggacgccgtt tgggctaccg
     1021 cggcggacgg cggccggggt ggacaccaac cggatgctgc tcctcctcgc ggtgctggag
     1081 aagcgggccg gactgcacct gatgagcttt gacgtctagg tgagggtggc tggtggcgtg
     1141 cgggtcgacg agccggcgct ggatctggga gtggcgtgcg cggtcgcgag cagccaccgc
     1201 gagcgcccgg ccgaccccgc cacggtggtc gtgggcgagg tggggctcgc cggcgaggtg
     1261 cgggcggtca gccggatcgc cagccgggtc gcggaagcgg agaagatggg attccggcgc
     1321 gtgatcctcc cgcgggccaa cctgccggga atcgaggccc ccggggagat cgaactgatc
     1381 ggggtcgggg agattcgaga ggccctgaac ctcgtggtcg gctgagccgg cgcctcaggt
     1441 gaggttaaag accccgaggg tgttgatccg gctgtgttgc ttctcctgga tgctctccag
     1501 ctccaggttg aggaggttgc acagccccgc ggtgtagaac agcgacgatc cgatctctgt
     1561 ctcga
//
LOCUS       DASQGH010000151         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34558, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000151 DASQGH010000000
VERSION     DASQGH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34558"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1047
                     /gene="ispE"
                     /locus_tag="VEW91_02830"
     CDS             181..1047
                     /gene="ispE"
                     /locus_tag="VEW91_02830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYM90554.1"
                     /translation="MKQLRLNAYAKINLTLDVLGDRPDGYHDIETVLHTVELHDTIIL
                     REDGEGITLRCANPGVPAETQNIVHRAAHLLRETYQIPRGIEIELTKRIPVASGLGGG
                     SSDAAVTLLGLAQMWKLRVDDRQLMELGSKIGSDVPFFLIGGAALAMGRGERVRMLRP
                     LPTTWVVIARPTIEITSEWAYKALDHGAVRKRPDTSGVVRALESEDARAVGQLLCNVF
                     EDVVIAHHPVVGTIRERMVAHHPLGTALSGTGPALFAMTANEAAARAMAAAIGRMADV
                     EVFVTRTFAEER"
     gene            1056..1847
                     /locus_tag="VEW91_02835"
     CDS             1056..1847
                     /locus_tag="VEW91_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HYM90555.1"
                     /translation="MPDALVLAGGQADPALAPGVPNKAFTPLAGRPMVEFVLRALRGA
                     SSVRRIALVGPLPLPPALAAYVDLAIEDQGALLDNVAAGLAALGGAGWVLTAGADIPL
                     LTPRAVDAFVEAARAIDAEIVYGVVRREDVLREAPAIRKTFVRLREGTLTGGSLVLLD
                     PRAFARARSAIDRAVRARKRPWDLARLFGLRTVFGLLTGTLRIPELEDRVARLAGVRV
                     RALVCPAPEIAIDVDSPESLVLVRLRLAGGSGSPAPDPTERAARP"
     gene            1844..2677
                     /gene="purR"
                     /locus_tag="VEW91_02840"
     CDS             1844..2677
                     /gene="purR"
                     /locus_tag="VEW91_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HYM90556.1"
                     /translation="MRRLRRGDRMVAIAHRLLQEPRTVFPLGTFTELFGAAKSTISED
                     IASLRQAFDRFAFGRIETLAGATGGVRFTPHTTPEQTRALAEDLARRLRDPSRILPGG
                     FLYIADLLSLPSLVSRLGDVFATFFADRRPDVVLSMEVQGIPLALMTARAFNVPLVTA
                     RRGGMGTGGPSVGVNYASGSSQTVQSMTLPLRAVPPGARALFIDDFLRGGGTARGVHD
                     LMREFQAEIVGIGVLIETTQPKEKLVGQYVSLLVFDGVDEAAGLIRVGPSRWALGDPA
                     R"
     gene            complement(2704..3282)
                     /locus_tag="VEW91_02845"
     CDS             complement(2704..3282)
                     /locus_tag="VEW91_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HYM90557.1"
                     /translation="MHDTAYIAPTAVVCGDVSIGEGSRVLFGAVIVAEGGPVEIGAYC
                     IVMENAVVRGTPRHPARLGNHTLVGPHAHLTGCTVDDNVFLATGTAVFNGARIGSRAE
                     VRINGVVHVEARVLPNAIVPIGWIAVGDPAQIVPPQEHDRIWSAQGPATFPLTVFGLE
                     RAPARETIMPELTRRYARALGLHREDHVVERA"
BASE COUNT          447 a         1179 c         1181 g          479 t
ORIGIN      
        1 ccaaaccgcg gcgagcgggg agagggggcg gggaccgcgc cggcggtgga ggatggccgg
       61 ggccggatcg cgaagaacag cccgatgcac cgcaccggaa gatgcgaacc ccgcgaggcc
      121 gcgcacgctc ggccccgcgg ggtgcgtttt tgagaggggt gtaagggggg gagtgccgcc
      181 ttgaagcagc tacgcctcaa cgcctacgcc aagatcaacc ttacgctcga cgtgcttggc
      241 gatcgccccg acggttacca cgacatcgaa accgtgctgc acaccgtgga actccacgac
      301 acgatcatct tgagggagga cggggaaggc atcacgcttc gatgcgccaa cccgggcgtc
      361 cccgccgaga cccagaacat cgtgcaccgc gccgcgcacc tgttgcggga gacctaccag
      421 atcccccgcg ggatcgagat cgagctgacc aagcggatcc cggtcgcctc cgggctcggc
      481 ggcgggtcga gcgacgccgc cgtcacgctc ctcggcctcg cccagatgtg gaagctgcgc
      541 gtggacgacc ggcaactgat ggagctgggc agcaagatcg gctcggacgt cccgttcttc
      601 ctcatcggcg gggcggccct ggcgatgggg cggggggaac gggttcgcat gctcaggccg
      661 ctgcccacga cctgggtggt gatcgcccgg ccgacgatcg agatcaccag cgaatgggcc
      721 tacaaggcgc tcgaccacgg ggcggtgcgc aagcgccccg acacctccgg ggtcgtccgg
      781 gccctcgaga gcgaagatgc gcgcgcggtc gggcagctcc tgtgcaacgt gtttgaggac
      841 gtcgtgatcg cgcaccaccc cgtcgtgggg acgatccgcg agcggatggt cgcgcaccac
      901 ccgctcggca ccgccctctc cgggaccggg cccgcgctct tcgccatgac cgcgaacgaa
      961 gccgcggcta gagcgatggc cgccgcgatc gggcggatgg ccgacgtcga ggtgttcgtg
     1021 acccggacct ttgccgagga gcgatagcgc acggaatgcc cgacgcgttg gtgctggcgg
     1081 gcggacaggc cgatcccgcg ctcgcccccg gcgtccccaa caaggcgttc acccccctcg
     1141 ctggccggcc gatggtggaa ttcgtgctgc gggcgcttcg gggggcgtcc tcggtccggc
     1201 ggatcgcgct cgtcggtccg cttcccctcc ctcccgcgct tgcggcgtac gtggacctgg
     1261 cgatcgagga tcagggggcg ttgctcgaca acgtggccgc ggggctggcg gcgctcgggg
     1321 gcgccggctg ggtgcttact gcgggcgcgg acatccccct cttgacccct cgggcggtcg
     1381 acgcctttgt ggaggcggcg cgggcgatcg acgccgagat cgtctatggc gtcgtccgcc
     1441 gcgaggacgt cctgcgggaa gcgcccgcga tccgcaagac gttcgtgcgc ctgcgcgaag
     1501 gcacgttgac cgggggtagc ctcgtcctcc tcgatccccg ggcgttcgcg cgcgcccgga
     1561 gcgccatcga ccgggccgtc cgcgcccgga agcgtccgtg ggaccttgcc cgcctgtttg
     1621 gcctgcggac ggtgttcggg ctgctcacgg ggaccctccg gatccccgaa ctcgaggacc
     1681 gggtcgcccg cctggccggg gtccgggtcc gggcgctcgt ctgcccggcg cccgagatcg
     1741 cgatcgacgt cgactcgccg gagagcctgg tgctcgtccg cctgcgcctg gccgggggct
     1801 ccggatcgcc tgccccggat ccgacggagc gggcggcgcg gccgtgaggc gcctcagacg
     1861 gggggatcgc atggtggcga tcgcccaccg gctgctccag gaaccgcgca cggtcttccc
     1921 gctcgggacc ttcaccgagc tgttcggcgc ggccaagtcg acgatcagcg aggatatcgc
     1981 ctcgctccgg caggcctttg accggttcgc cttcggccgg atcgagacgc tcgcgggcgc
     2041 aacggggggc gtgcgcttca cgccccacac caccccggag cagacccgcg cgctggcgga
     2101 agacctggcc cggcgcctcc gggacccatc gcggattctg cccggcgggt tcctctacat
     2161 cgccgacctc ctctcgctgc ccagcctggt gtctcgattg ggcgacgtgt tcgcgacgtt
     2221 cttcgcggac cggcggccgg acgtcgtgct ctcgatggag gtgcagggga tcccgctcgc
     2281 gctcatgacc gcgcgggcgt tcaacgtccc gctcgtcacc gcccggcggg gcgggatggg
     2341 aaccgggggc ccctcggtgg gcgtcaacta cgcctcgggg tcctcgcaaa cggtgcagtc
     2401 gatgacgctg cccctgcgcg cggttccccc gggtgcccgc gccctgttca tcgacgactt
     2461 ccttcggggc ggcggcaccg cccgaggcgt ccacgacctc atgcgcgagt ttcaagccga
     2521 gatcgtcggg atcggggtct tgatcgagac gacgcagccc aaggaaaagc tcgtcggtca
     2581 gtatgtgtcg ttgctggtct tcgacggcgt cgacgaggcg gccggcctca tccgcgtcgg
     2641 tcccagccgg tgggccctgg gcgacccggc ccggtaagcg acggcctcga ccccgccgcg
     2701 cgcctacgcc cgctccacga cgtgatcctc ccggtgaagg ccgagcgcgc gggcgtagcg
     2761 gcgcgtgagc tcgggcatga tcgtttcacg cgccggggcc cgctcgagcc cgaagaccgt
     2821 cagcgggaag gtcgccggcc cctgcgccga ccagatgcgg tcgtgctcct gcggcgggac
     2881 gatctgggcg gggtcgccca ccgcgatcca gccgatcggg acgatcgcgt tgggtaacac
     2941 cctggcctcg acgtggacca ccccgttgat gcgcacctcc gcccggctac caatccgcgc
     3001 gccattgaag accgcggtgc ccgtggccaa aaagacgttg tcgtccaccg tgcatccggt
     3061 gaggtgcgcg tggggcccga ccagggtgtg gttccccagc cgcgcggggt gccggggggt
     3121 gccccggacg acggcgttct ccatgacaat gcagtaagcc ccgatctcca cgggcccgcc
     3181 ctccgccacg atcacggcgc cgaacaggac ccggctgccc tcgccgatcg agacgtcccc
     3241 gcacaccacc gccgtcgggg cgatataggc ggtatcgtgg atcgcc
//
LOCUS       DASQGH010000152         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37503, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000152 DASQGH010000000
VERSION     DASQGH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37503"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VEW91_02850"
     CDS             complement(<1..594)
                     /locus_tag="VEW91_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYM90558.1"
                     /translation="MKINCCWLYAISKYGYPPSIPDTHRALEEMAALGFDAAELEGVH
                     EENLRAVATVRSEIRRRCADLGLRIVNFCPVLPDLVSPDLPQRRHALDLFRLGAELAV
                     YFGSPLIQVDSYTPPIEFVEHRPYGTTIEFQRQFDVRIPAEFSWDRTWNVLEDSVRTC
                     AAIAREAGLQLAMEPRVGEIVSNTDALLRLMDHVADPS"
     gene            complement(591..1853)
                     /locus_tag="VEW91_02855"
     CDS             complement(591..1853)
                     /locus_tag="VEW91_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HYM90559.1"
                     /translation="MAGYGARSGPARGVHDDLFGRCLVLEDASWAAALVEADVLQLPK
                     TFTDHVRLQVARRTGIPREHVLVCATHTHSGPDMEGRYTPGGQDRSLAEVWQRALAGC
                     AEAAWRARQDAAVGIGVGSVQGIGVNRRTQDGQPVDPMVGVISARGQAGVLLGVLFNY
                     TCHPVVLGGDNLLFSADYPGYAIRAIEAIEGPHTIAMFTNGAEGDVNTGHSADLSGIG
                     APIPERTFERAQRLGRRLAGEVLKVLSGTPTPLTGPVRAIVQEVTLPFRHVASVERAE
                     AALAAAEREVRQLMAAGADPEATTSARIRRFHAEVDLMVATQRAGETESGLVAELQAI
                     RVGDLALVAIPGELFVELGLAIKAGSPFDYTLMLGLANGSVGYLPTREACQAGGYEAV
                     ATRFAPGTGELVRDTCLEILHTLKEATP"
BASE COUNT          368 a          665 c          618 g          330 t
ORIGIN      
        1 tgacggatca gcgacgtgat ccatcaaccg gaggagcgca tcggtgttgc tcacgatctc
       61 accgacacgc ggctccatcg ctagctgcag gcccgcctct cgcgcgatcg cggcacaggt
      121 gcggacggag tcctccagca cgttccatgt gcggtcccac gaaaattctg ccggaatgcg
      181 cacgtcgaac tgccgctgga actcgatcgt ggtaccgtag ggtctgtgct ccacgaactc
      241 gattgggggc gtatagctgt cgacctgaat cagcgggctc ccgaagtaca ccgccaactc
      301 ggcaccgagg cgaaacaagt ccagcgcatg gcggcgctgc ggtagatcgg gactaaccag
      361 gtcgggtagc acagggcaaa agttcacgat gcgcagcccc aggtcggcgc accgacgccg
      421 gatctccgat cgcaccgtcg cgacggcacg gagattctct tcatggacgc cctcaagttc
      481 agccgcgtca aaccctagcg cggccatttc ctcgagggcc cggtgggtgt cgggaatcga
      541 cggcggatag ccgtacttgc tgattgcgta cagccagcaa cagttaatct tcatggggta
      601 gcctccttca aggtgtgaag tatctcgagg catgtatcgc gcacgagttc cccggtgcca
      661 ggggcaaagc gcgtggccac cgcttcgtac cctccggcct ggcaggcttc ccgggtgggg
      721 agatagccga cagagccgtt ggcgaggccg agcatcaagg tgtagtcgaa aggcgatccc
      781 gccttgatgg cgagcccgag ctccacaaag agctcgcctg gaatggccac gagggcaaga
      841 tcgccaacgc ggatcgcctg gagctcagcc accagacccg actcggtctc cccggctcgt
      901 tgggtggcga ccatgagatc cacctcagca tgaaatcgac ggattcttgc agaggtggtt
      961 gcctcggggt cagcccccgc tgccatgagc tggcgtacct cgcgctccgc tgccgcgaga
     1021 gcagcctcag cgcgctcaac gctcgcgacg tggcggaacg gcagcgtcac ctcttgtacg
     1081 atggcccgca cggggcccgt caggggcgtc ggggtccctg agagcacctt gaggacctct
     1141 cccgccaatc ggcgaccgag acgctgcgca cgctcgaagg ttcgctcggg gatgggcgcc
     1201 ccgatcccgc tcaggtccgc cgaatggccc gtgttcacgt ccccttcagc cccattggtg
     1261 aacatcgcga tggtgtgtgg gccctcgatc gcttcaatcg ctcggatcgc atatccagga
     1321 taatctgcgg aaaagagaag attatcgcct ccgagcacga ccgggtggca cgtgtaattg
     1381 aatagcaccc cgaggagcac accggcctgg ccgcgggcgg agatcacccc caccatcgga
     1441 tccaccggtt ggccgtcttg agtccgtcga ttcacgccaa tcccctggac cgatcctacg
     1501 ccgatcccaa ctgctgcgtc ctgacgagcc cgccacgcgg cctctgcgca tcccgcgagg
     1561 gcccgctgcc atacctccgc caacgagcga tcctggccac ccggcgtgta gcgcccctcc
     1621 atgtccggac cggaatgagt gtgcgtggcg caaacgagga cgtgttcgcg gggaatccct
     1681 gttcgacggg caacctgcag ccgcacgtgg tcggtgaagg tcttgggaag ctgcaggacg
     1741 tccgcctcta cgagagcggc agcccaagac gcgtcctcga gcacgaggca acgcccgaat
     1801 aggtcgtcgt ggacgccgcg cgcaggaccc gaccgtgctc cgtacccggc catgtacgtg
     1861 ccgatcgggg gcgtgatagt gacgccgtaa aagccagcct tcacccccgc gcccctccgt
     1921 ctctagccct tcagtccggt cagggccacg ctctcaatga tgcgccgtcg caaaatgagg
     1981 a
//
LOCUS       DASQGH010000153         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20496, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000153 DASQGH010000000
VERSION     DASQGH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20496"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VEW91_02860"
     CDS             complement(<1..1540)
                     /locus_tag="VEW91_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HYM90560.1"
                     /translation="MAGSDDAYLAREAKARKKIDEQLMAAGWVVQGKDEIDVHAAEGV
                     AIREFVLEKPHGRVDYLLFIDGQPAGVIEAKPDGTTLVEVETQSGKYVDGLSDWMKPP
                     VYPLPFIYESTGTETRFTNGYDPDARSRRVFSFHRPETLAEWARQIRENPDAPTFRAR
                     LKSLPELDSTRLWDVQATAISNLEESLRDDRPRALIQMATGSGKTFTAANISYRLIRH
                     AHAKRILFLVDRSSLGEQAKLEFDKFDVPDTQRKFPAEYNIQHLKSNTVDSTSRVCIS
                     TIQRIFWILRGEEGEMDEETDEHSVYELPVSQPVKVEYNPALPPDAFDVIIVDECHRS
                     IYGVWRQVLEYFDAHLVGLTATPTKQTYGFFRQNLVMEYSHELAVADGVNVDFTVFII
                     KTAITEGGSRIETGEFAGYRDRMTRKLRWEAVDEPIEYTGTQLDRAVVAEDQIRTVLT
                     TFKEKLFTELFPGRTTVPKTLIFAKDDSHADDIVQMVREVFGKGNEFATKITYRSRDG
                     KAKEL"
     gene            complement(1796..1869)
                     /locus_tag="VEW91_02865"
     tRNA            complement(1796..1869)
                     /locus_tag="VEW91_02865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1833..1835),aa:Met,seq:cat)
     gene            complement(1979..>2566)
                     /locus_tag="VEW91_02870"
     CDS             complement(1979..>2566)
                     /locus_tag="VEW91_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM90561.1"
                     /translation="AAAAGELAQRTGGRVRPVTLDVADPASIAAAAESVRADPGRLDV
                     LVNNAGIGSDFGVSGVEPDFEAIQRALDTNFYGAYRLMVALLGLLRQSGHPRIVNVSS
                     GMGGVAEMGGWSPGYRVSKAALNAMTRILSTELKEEGFLVNSACPGFVNTDMGGPMGA
                     TKPVEEGAAGIVWLATLPDDGPTGGFFRDGQPVAF"
BASE COUNT          433 a          916 c          746 g          471 t
ORIGIN      
        1 gcagttcctt ggccttaccg tcgcgactac ggtaggtgat cttcgtggcg aactcgttgc
       61 ccttgccgaa gacttcgcgg accatctgca cgatgtcgtc ggcgtgggag tcgtccttgg
      121 cgaagatcag cgtcttgggc acagtggtgc gtcccgggaa cagctcggtg aacagctttt
      181 ccttgaatgt ggtgagtaca gtgcggatct gatcctcagc gaccaccgca cggtcgagct
      241 gtgtgccggt gtattcgatc ggttcgtcca ccgcttccca gcgcagcttt cgcgtcatcc
      301 ggtcgcgata gccggcgaac tcccccgtct caattctcga tccgccctcg gtgatcgcgg
      361 tcttgatgat aaagaccgtg aaatccacgt tcacgccgtc cgcaaccgcc agctcgtggc
      421 tgtactccat cacgaggttc tggcggaaaa acccatacgt ctgcttcgtc ggcgtcgccg
      481 tcagcccaac caggtgcgca tcgaagtact cgagcacctg gcgccacacc ccgtagatcg
      541 agcggtggca ctcatccacg atgatcacgt cgaacgcgtc cggcggaagc gccggattgt
      601 actcgacctt taccggctgc gacaccggca gttcatatac ggagtgctcg tcggtctcct
      661 catccatctc cccctcctcg ccacgcaaga tccagaagat ccgctggatc gtcgaaatgc
      721 acacgcgcga tgtggaatcg accgtgttcg atttgagatg ctggatgttg tattccgccg
      781 ggaacttccg ctgcgtatcc ggaacatcga acttgtcgaa ctcgagcttt gcctgttcgc
      841 cgagactcga gcgatcgacc agaaatagga tccgcttcgc atgcgcgtgc cggatcagtc
      901 gataggagat gttggccgcg gtgaacgtct tccccgaccc cgtcgccatt tggatcagcg
      961 cacgtgggcg atcgtcgcgc aacgactcct ccagattgga gatcgccgtc gcctgaacat
     1021 cccacagcct tgttgagtcg agctccggca acgactttag ccgcgcacga aaggtcggag
     1081 cgtccgggtt ctcgcgaatc tggcgagccc actcggcgag tgtctcgggc cggtggaacg
     1141 agaacacgcg gcggctgcga gcatccgggt catagccatt cgtgaagcgc gtctcggtgc
     1201 ccgtcgactc gtagatgaat gggagggggt ataccggtgg cttcatccag tcggataggc
     1261 cgtcgacgta cttaccgctc tgggtctcca cctcgaccag cgtcgtgcca tccggcttgg
     1321 cctcgatcac ccctgctggc tggccgtcga taaacagcag gtagtcgacc cgaccgtgcg
     1381 gcttctccag gacgaactcc ctgatcgcaa caccctcggc cgcatgcacg tcgatttcgt
     1441 ctttgccctg aacgacccag cccgccgcca tcagctgctc gtcgatcttt ttgcgcgcct
     1501 tggcctcgcg ggccaggtag gcatcgtctg agcccgccat tgacgctgga ttctacctgc
     1561 cgagatcaag ggaggaacct gcccaccacg tctcccgcgg cagcatgcgg ggaccaagct
     1621 ccggaggacg ggataccatc gcatccgcgg cgcgccctga atcccagaag cccgagcatg
     1681 gtgcacacga tttccatgca acgccttgac cccagcttga cccgagggga gatgacggcc
     1741 gactcgtgac ccacctgcat acgaaaaaac cccgccgtag cggggctctt tcgcatagcg
     1801 ggggcgggat tcgaacccgc gaccttcggg ttatgagccc gacgagctac cagactgctc
     1861 caccccgcgt cgctccttat gttctagcaa acaaacccgt ccgaggccag gttgggcggg
     1921 cgccgacgag ggcgggcggg ggagcgccgc gcccgacggg ccaggccagg ggcgccgctc
     1981 agaacgccac cggctggccg tccctgaaga accctcccgt cgggccgtcg tccgggaggg
     2041 tggccagcca cacgatcccc gccgccccct cctccaccgg cttggtcgcc cccatcgggc
     2101 cgcccatgtc cgtgttcacg aacccggggc aggcagagtt cacgaggaag ccctcctcct
     2161 tcagctccgt cgacaggatc ctcgtcatcg cgttcagggc cgccttcgac acccggtacc
     2221 cgggcgacca gccccccatc tccgccaccc ctcccattcc gctcgacacg ttcacgatcc
     2281 gcgggtgccc gctctggcgc agcagcccca gcagggccac catcagccgg taggccccgt
     2341 agaagttcgt atccagcgcc ctttggatcg cctcgaagtc cggctccacc cccgacaccc
     2401 cgaagtcgct gccgatgccc gcgttgttca ccagcacgtc cagccggccg gggtccgccc
     2461 gcacgctctc cgccgccgcc gcgatgctcg ccgggtcggc cacgtccagc gtcaccggcc
     2521 tcaccctccc gcccgtcctc tgcgccagct cccccgccgc cgcggc
//
LOCUS       DASQGH010000154         5411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31861, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000154 DASQGH010000000
VERSION     DASQGH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31861"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VEW91_02875"
     CDS             <1..165
                     /locus_tag="VEW91_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90562.1"
                     /translation="TTIFMVEQNANMALSIATRGYVLQTGQVVLSGTAQSLRENEMIR
                     SAYLGEMRTA"
     gene            320..2098
                     /gene="dnaG"
                     /locus_tag="VEW91_02880"
     CDS             320..2098
                     /gene="dnaG"
                     /locus_tag="VEW91_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HYM90563.1"
                     /translation="MAGAREVTDEIRRRVSLLDVVSSHVTLRRSGRSYKGLCPFHSEK
                     TPSFTVDPERGFFYCFGCHAGGDVFDFVMRTGALSFGEARQELAARAGIPLEAVPGGE
                     RGSGERERLLRAVAEAAAFFQAQLAGAAGEGARRYLASRGVDPATCLAFRLGFAPAGW
                     DNLTRALLARGFDGAALEQAGLAVARPGDGGHYDALRGRLVFPIHDLQGRPVAFGGRA
                     LDNGMPKYLNTRETVLFAKGKMLYALSEAREQLRETRDAVVVEGYMDALTCHQYGVRN
                     AVASLGTALTLDQVLLLKRFAARAILVYDSDAAGIGAAERGLDVFDQAELPVRVVVLP
                     GESDPDAFLRREGPEAFRRACAAALPIFDYRWAMAQQRYDSRTVEGKVGIVNELGPLI
                     ITASNPVRQGEYVRLLADRLGVREDAIRGQLGRIGRGRPGTKEPGRQNAMPPVPDANA
                     RVLTERELLHLLVASPAVRASLQGAVTPEVFTEPEHRELAAVLLAGAGEVIDPGRLRE
                     RLRGETAVSLLSRFLIAEPHARDPLQAARACVQRIRRIGLQGRVDGLLGALREADQTR
                     DRERVEALKVELQELYRELNVGAQAP"
     gene            2145..3233
                     /gene="rpoD"
                     /locus_tag="VEW91_02885"
     CDS             2145..3233
                     /gene="rpoD"
                     /locus_tag="VEW91_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HYM90564.1"
                     /translation="MDAEKVLSTLSAELRQLVEQGRKRGYVTFDEIQEVVPEVEHNLD
                     EYDRIYGIFVDLDIKVVDDPKEAEKRSDDEEEPEAVLEPLEGVSIDDPVRMYLKEIGK
                     VPLLNSADEIKLAKRMERGDEEAKRRLIEANLRLVVSIAKKYVGRGMLFLDLIQEGNL
                     GLIRAVEKFDWRKGYKFSTYATWWIRQAITRALADQARTIRIPVHMVETINKLIRISR
                     QLLQELGREPTPEEIGQVMKMPTERVREIIKIAQEPISLETPIGEEEDSHLGDFIEDQ
                     DALAPAEAASFTMLKEQLEGVLETLTPRERKVLKLRFGLDDGRPRTLEEVGQEFGVTR
                     ERIRQIEAKALRKLRHPSRSKRLKDFIE"
     gene            complement(3223..4383)
                     /locus_tag="VEW91_02890"
     CDS             complement(3223..4383)
                     /locus_tag="VEW91_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYM90565.1"
                     /translation="MKVVGARIYLVAIGGRHPILVEVSTDQGISGVGEAAIAYGSGAT
                     AAAGMIKDLVEGFVLGKDPRRIEALWAEMYDHTFWAKGGGPIIFAGISAIEQALWDIK
                     GKALGVPVYELLGGRCRDAVRVYANGWSFRCITPDDFAREARRVVGDGYTALKFYPLA
                     SAELDEHGHIRHVSGRSLDRAAERLAVARVRAVREAVGPDVDLMLDMSAELTTDAILR
                     IGRQVEPENILFFEEPVDPFDVDALKKVSEHLDLPVAVGERLYTRYGFRRVLELHAAD
                     VLQPDIGTTGGIMEAKKIAAMAEAYSMRVAPHNCASPVSTAAALQLDACIPNFLIQEL
                     YPYRTPGHFAIVDRAPELDVRGGMLAIPDRPGLGVALVEERVRPFLWAELTR"
     gene            4792..5220
                     /locus_tag="VEW91_02895"
     CDS             4792..5220
                     /locus_tag="VEW91_02895"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HYM90566.1"
                     /translation="MHAQRGYTAIELSVALSLAGILVAGSVFSIRSSLAREALDGWAR
                     SMTLDIAAGQQAAVTLRATVNITVTTTSYTMAVSGGNTFRNATLPADISLTTTCSSNV
                     CAFDRRGVPLAAGTITLTSALAKRSYVVTINPTTGSVSYQ"
     gene            5217..>5411
                     /locus_tag="VEW91_02900"
     CDS             5217..>5411
                     /locus_tag="VEW91_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYM90567.1"
                     /translation="MMRLHRILRRSQAGQRGFTVVETVVAMALLGILTISALGGLLFG
                     MTQVHTGQNRAAVASWGQTEM"
BASE COUNT          931 a         1719 c         1896 g          865 t
ORIGIN      
        1 acgacgatct tcatggtcga gcagaacgcc aacatggcgc tctcgatcgc cacccggggc
       61 tacgtgctgc agaccggcca ggtggtgctc tctgggacgg cgcagagcct cagggaaaac
      121 gagatgatcc ggagcgccta cctcggggaa atgagaacgg cctgaaggag acggccgcat
      181 ccctgcgtct ccagcaaggg ggtccgtgca tcggggaaga ggactgggat gtggaagtgg
      241 acaatatatt cagtaggggg cgcggaaagc cttatcttgc gcagagatga ggcttttttc
      301 gcgtccggag gtgccaggcg tggccggagc tcgggaagtc accgacgaga tcaggcggcg
      361 ggtgagcctg ctggacgtcg tgtcgtcgca tgtcaccctg cgccgctccg ggcgcagcta
      421 caaggggctg tgccccttcc actcggaaaa gacgccgtcg ttcaccgtcg atccggagcg
      481 gggcttcttt tactgttttg ggtgccatgc cggcggggac gtgtttgact tcgtgatgcg
      541 tacgggcgcc ctctccttcg gggaggcgcg tcaggaactg gctgcgcgcg ccgggatccc
      601 cctggaggcc gtccccgggg gcgagcgcgg ctccggggag cgcgagcggc tgctgcgggc
      661 ggtcgcggag gcggcggcgt tctttcaggc tcagctggcg ggggccgccg gcgagggcgc
      721 gcgccggtac ctggcgtccc gcggcgtgga tcccgcgacg tgtctggcct tccggctcgg
      781 gtttgcgcct gccggctggg acaacctgac ccgggccctc ctcgcccggg gattcgacgg
      841 tgccgcactc gagcaggccg gcctggccgt cgcgcgtccc ggagatggcg gacactacga
      901 cgcgctccgg gggcgcctgg tctttcccat ccacgacctc cagggcaggc cggtggcctt
      961 tgggggacgc gcgctcgaca atgggatgcc gaagtacctc aacacccggg agacggtgct
     1021 gtttgcgaag gggaagatgt tgtacgccct cagcgaagcc cgggagcagc tccgcgaaac
     1081 ccgagacgcc gtggtcgtcg aagggtacat ggacgcgttg acctgtcacc agtacggggt
     1141 gcgcaacgcc gtggcctcgc tcggcacggc cctgacgctc gaccaggtgc tgctcctcaa
     1201 gcggttcgcc gctcgggcca tcctcgtcta tgactccgac gcagcaggga tcggcgcggc
     1261 ggagcgcggc ctcgacgtgt tcgatcaggc cgagttgccg gttcgggtgg tcgtgcttcc
     1321 gggggagtcc gacccggacg cgttcctccg gcgggagggc cccgaggcgt tccggcgggc
     1381 gtgcgccgcc gcgctgccga tcttcgacta ccggtgggcg atggctcagc agcggtacga
     1441 cagccggacg gtggaaggaa aggtcggaat cgtcaacgaa ctgggaccgt taatcattac
     1501 ggcgtccaat cccgtgcgtc agggggagta tgttcgtctc ctcgcggacc ggttgggcgt
     1561 gcgggaggat gcgatccggg gtcaactggg gcggatcggc cggggacggc cggggacgaa
     1621 ggaaccgggt cggcagaacg cgatgccgcc ggttccggac gcgaatgccc gagtcctcac
     1681 cgaacgcgag ctccttcacc tgttggtggc cagtccggcg gtgcgcgcgt ccctgcaggg
     1741 ggccgtgacc cccgaggtgt tcaccgagcc ggagcatcgt gagctggcgg ccgtgttgtt
     1801 ggccggcgcc ggtgaggtta tcgatccggg gcggttgcgc gaacggctac ggggcgagac
     1861 ggcggtctcg ctcctgagcc gctttttgat tgccgaaccg catgcgagag atcccctcca
     1921 ggcggctcgg gcatgcgtgc agcggattcg acggatcggg ctgcaggggc gcgttgatgg
     1981 gttgttgggg gcgctccggg aggcggacca aaccagggat cgggagcgcg tggaggccct
     2041 gaaggtggaa ctccaagaac tctacaggga gttgaatgtc ggtgcccagg ccccgtaagg
     2101 gacagacatc cggggctggc gcgagcgccc cggcgaagcg aagggtggac gccgagaagg
     2161 tgctgagcac cctctccgcg gagttgcggc aactggtcga gcagggccgc aagcgcgggt
     2221 acgtcacctt tgacgagatc caggaggtcg tgcccgaggt cgagcacaac ctcgacgagt
     2281 acgaccgcat ctacgggatt tttgtcgatc tcgacatcaa ggtggtggac gacccgaagg
     2341 aggccgagaa gcggtccgac gacgaggaag agcccgaggc ggtgctcgag cccctggaag
     2401 gggtcagcat cgatgacccg gtccggatgt acctcaagga gatcggcaag gtcccgctgc
     2461 tcaactcggc cgacgaaatc aagctggcca agcggatgga gcgcggggac gaggaagcga
     2521 agcgccgcct catcgaggcc aacctgcgcc tggtcgtcag catcgccaag aagtacgtcg
     2581 gccgcggcat gctgttcctg gacctgattc aggaagggaa cctcggcctg atccgggccg
     2641 tcgagaagtt cgactggcgc aaaggctaca agttcagcac ctatgcgacc tggtggatcc
     2701 gtcaggccat cacccgggcg ctggccgacc aggcacggac catccgcatc cccgtccaca
     2761 tggtcgagac gatcaacaag ctgatccgga tctcccgcca gctcctccag gaactggggc
     2821 gggagccgac ccccgaggag atcggccagg tgatgaagat gccgaccgag cgggtccggg
     2881 agatcatcaa gatcgcccag gagccgatct cgctcgagac gccgatcggc gaagaagagg
     2941 acagccacct cggggacttc atcgaggatc aggacgcgct cgccccggcc gaggccgcct
     3001 cgtttacgat gctcaaagag cagctcgagg gcgtcctcga gaccctcacg ccccgggagc
     3061 gcaaggtgct caagctccgg ttcggcctcg acgacggccg gccccggacc ctggaggagg
     3121 tcggtcagga gttcggggtc acccgcgagc gcattcggca gatcgaagcg aaggcgctgc
     3181 ggaagctccg ccaccccagc cggagcaagc ggctcaagga cttcatcgag taagctccgc
     3241 ccacaggaac ggccggaccc gctcctccac caaggccacg cccagtcccg ggcggtccgg
     3301 gatcgcgagc atgccgccgc ggacgtccag ctccggggcg cggtcgacga tcgcgaagtg
     3361 ccccggggtg cggtacgggt acagttcctg gatgaggaag ttcgggatgc aggcgtccag
     3421 ctgcagcgcc gcagccgtgg agaccgggct ggcgcagttg tggggcgcca cccgcatgct
     3481 gtacgcctcg gccatcgcgg cgatcttctt cgcctccatg atcccgccgg tggtgccgat
     3541 gtccggctgg aggacgtcgg ccgcgtggag ctcgagaacc cggcgaaagc cgtaccgggt
     3601 gtagaggcgc tcgccgaccg cgaccggaag gtcgagatgc tcggagacct tcttcagcgc
     3661 gtcgacgtcg aacggatcga ccggctcctc gaagaacaag atgttctcgg gttcaacctg
     3721 ccggccgatg cggaggatgg cgtcggttgt cagttcggcg ctcatgtcca gcatcaggtc
     3781 gacgtcgggc cccaccgcct cgcgaaccgc ccgcacccgc gcgacggcca ggcgctccgc
     3841 cgcccggtcc agggatcgac ctgacacgtg cctgatgtgc ccgtgctcgt ccaactcggc
     3901 agaggcgagg gggtagaact tgagcgcggt gtatccatcc ccgactacgc gccgcgcttc
     3961 gcgggcgaaa tcgtccggcg tgatgcaccg gaacgaccag ccgttggcgt acacccggac
     4021 cgcatcccgg caacggcccc cgagcagttc gtagaccggg acgcccagcg ccttgccctt
     4081 gatgtcccac agggcctgct cgatggcgct gatcccggca aaaatgatcg ggcccccgcc
     4141 cttggcccaa aacgtgtggt cgtacatctc ggcccaaagg gcttcgatcc gccggggatc
     4201 cttgccgagc acgaagccct cgaccaggtc cttgatcatg cccgccgcgg ccgtcgcgcc
     4261 cgagccgtag gcgatcgctg cttctcctac gccgctgata ccctggtcgg tcgacacctc
     4321 caccaggatc gggtgccgtc ctccgatcgc cacgagatag atcctggccc cgacgacctt
     4381 catcggccca cctcgaggtg gagcactacc acggagggga gctgcctcct gcagatgacg
     4441 cgaacgaggg gagacaggcc gcgggcagac ttccacaaaa gttaagacgc ttcgacgaca
     4501 atcttccccg aatcttacca caccccgcgt cgatcggccg gactctgatg ctgtccagga
     4561 cccaccgaac ggatgggatc agcctatcga atggtgaagc aacccgtcaa acggatgagt
     4621 ccggtctcct tcgaaagcgg gcaagaacta atgtgaggat gatggtcatg gcgccgagcc
     4681 ccccagtcct gtgctggtta cacggggcgt attgagacga ccatgtgccg tcaacgtcga
     4741 ctgggcgtgg tgacgttggg agtggcaaag acaaccgggg cgaagcgacg cgtgcacgcc
     4801 caacgtgggt acacggccat cgaactcagc gtagcgcttt cactggcggg gatccttgtg
     4861 gccgggagcg tgttcagcat ccggtcttca ttggcccggg aagcgctcga tggatgggcg
     4921 cgatcgatga cgttggatat cgcggcgggg cagcaggcgg cggtgacctt gcgggcgacg
     4981 gtcaacatca ctgtgaccac gacatcttac accatggccg tcagcggggg taatacgttt
     5041 cggaacgcga cgctgccggc ggatatttcg ctcaccacca cgtgttcatc gaatgtctgc
     5101 gcgttcgatc ggcggggggt gccccttgcc gcgggcacga tcacgctcac gagtgcgttg
     5161 gccaagcgca gctatgtggt caccatcaac cccactaccg ggagcgtgtc ttaccaatga
     5221 tgcgtcttca taggatcctg cgccgctccc aggccggtca gcgtgggttc acggtcgtcg
     5281 agacggtcgt cgcgatggcc ttgctcggca tcctcaccat ctcggcgctc ggaggcctgc
     5341 tgtttgggat gacccaggtc cacaccggcc aaaatcgggc ggccgtcgcc tcctgggggc
     5401 agacggagat g
//
LOCUS       DASQGH010000155         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9190, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000155 DASQGH010000000
VERSION     DASQGH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9190"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..358
                     /locus_tag="VEW91_02905"
     CDS             14..358
                     /locus_tag="VEW91_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90568.1"
                     /translation="MFLGRDLVESRNYSDEIAYEIDKEVRRIIDECYGRARAVLIDHR
                     EILDRIAKALLERESLESDAIDALIAGEPLPAEVPAAPPPVVAATQEVKSFPRPEAAI
                     PTKPKLKPETTG"
     gene            436..1032
                     /locus_tag="VEW91_02910"
     CDS             436..1032
                     /locus_tag="VEW91_02910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HYM90569.1"
                     /translation="MVEVPAYTIEPRVEVSPEHTALIVVDMQNDFVDPRGSLCVGTAR
                     ATVPAIARLCTFARAHGLTVVYTQDWHRPDDPEFALWGAHAVAGTWGAEIVPDLAPRP
                     EDLVVRKLRYDAFYGTSLDHELRQRRIAAVIVTGTVSNICVLHTAGSAALRWLQVIVP
                     VDAVSALTEFDQQAALRQIAFLYRGTLTQSENIVAKAG"
     gene            1060..1512
                     /locus_tag="VEW91_02915"
     CDS             1060..1512
                     /locus_tag="VEW91_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90570.1"
                     /translation="MPRSRKPKPFAWRPVAIYVAVVLVAGAGLVLWDRAYEARLSPSV
                     SIQPEVVAKNLVEALAGAGAVQSAKLDRKTGTLTMVVKDVLTDTGKPPAQNREQLSRQ
                     GSLEVESILGMITFKQIVLQIVKDGKVLATVRGEPGKPPQTEFAPDLK"
     gene            complement(1535..2266)
                     /locus_tag="VEW91_02920"
     CDS             complement(1535..2266)
                     /locus_tag="VEW91_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYM90571.1"
                     /translation="MARARMVAAGLIAAVALAGYAAAGTPASTAAPPSTPADSPPTSP
                     DQRIIVTGRGVVEAAPDRAIVTVGAQFTRATAQEAQEVASMTMTQTLRQVMALGIPRE
                     RIQTVEINLFPQRRPPAGEIVGYQAVQRARVTVDDLSLVGRVLDAAVTAGANLLDGVA
                     FTVHDPAASRTRAFAAAVQDARASANALAAAAGLATPRIVRIEELGATVPIRGEPLRA
                     APEVATPVLPGTLSISAQVRVVFAF"
     gene            2265..2756
                     /locus_tag="VEW91_02925"
     CDS             2265..2756
                     /locus_tag="VEW91_02925"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HYM90572.1"
                     /translation="MNAPPEDGLFLNTGIPRGMFPPDQDGGARGVWREARPETRNKRQ
                     MFALNFYSSVFTQALRDGRKTLTIRLGDKRDKYQAGQLVWVTVGNRFGAKQRIFTAMI
                     DRVEVKALREVTPREIERENPATREHTDLIEFLSKIYARKIGAEDTVTVIHFSRVTEA
                     KDE"
     gene            2802..>3718
                     /gene="ftcD"
                     /locus_tag="VEW91_02930"
     CDS             2802..>3718
                     /gene="ftcD"
                     /locus_tag="VEW91_02930"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HYM90573.1"
                     /translation="MPPSVIECVPNFSEGRRLPVLDALAASIRAVDAVRLLDVSSDPD
                     HNRAVFTFVGDGERVLEAAFRSAVVAIERIDLRTHRGVHPRIGAVDVIPFVPLLNATM
                     DACVALARRLGARLAADLDLPVYLYGRAGPQPSRSLAEIRRGGFEGLRMAIAQPSRAP
                     ELGPARVHPTGGAVAVGAREILIAFNVELQSTDVKVARTIARAVRESSGGLPAVQAMG
                     VPLASRGCVQVSMNLQDYRRTPPLVAFQRVEAEAARRGIAVSAGELVGCAPPEALPPD
                     PVAALRLRSLRPGQILDPARLARDLDGGGA"
BASE COUNT          571 a         1265 c         1331 g          551 t
ORIGIN      
        1 ctagcacggg ccggtgttcc tgggccggga cctggtggag agccggaact actcggacga
       61 gatcgcctac gagatcgaca aggaagtccg ccggatcatc gacgagtgct acggccgggc
      121 ccgggcggtg ctcatcgatc accgggagat cctcgaccgc atcgccaagg ccctgctcga
      181 acgggagtcg ttggagagcg acgcgatcga tgccctgatc gccggcgagc cgctgccagc
      241 ggaggtgccg gcggcgcccc cgccggtggt ggccgccacc caggaggtca agtcgttccc
      301 gcggccggag gcggccatcc ccacgaaacc caagctgaag ccggagacga ccgggtaggc
      361 gcggctgtgt tgggacagcg cgcggggaag gagccgtggc tgcttccccg cgcatgcatt
      421 taggagatgg ggcgcatggt cgaagtgccg gcctacacca tcgagccgcg cgttgaggtg
      481 agccccgagc acaccgcgct gatcgtggtg gacatgcaga acgatttcgt ggatcctcgg
      541 gggagcctgt gcgtggggac cgcgcgcgcg accgtgccgg ccatcgcccg tctgtgcacg
      601 ttcgcccgtg cacacggcct gaccgtggtc tacacccagg actggcaccg ccccgacgac
      661 cccgagttcg ccttgtgggg ggcgcacgcc gtggcgggga cgtggggagc cgagatcgtg
      721 ccagacctcg cgccccggcc ggaagatctg gtggtccgca agctgcgcta cgacgccttc
      781 tacggcacct cgctggacca cgaactccgc cagcgccgga tcgccgcggt gatcgtgacc
      841 ggaacggtga gcaacatctg cgtgctccac acggcgggga gcgccgcgct ccggtggctt
      901 caggtcatcg ttcccgtgga cgcggtctcg gcgctgaccg agtttgatca gcaggcggcc
      961 ctgcgtcaga tcgcctttct ctaccggggg acgctcacgc agagcgagaa catcgtggcg
     1021 aaggccggtt gacccgctcc gggcttggga gggatgccgg tgccgagatc cagaaaaccg
     1081 aagccgttcg cctggcgtcc ggtggcgatc tatgtcgccg tcgtcctcgt cgccggcgcc
     1141 gggctcgtgc tctgggaccg cgcctacgag gctcggctga gcccgtctgt ctcgatccag
     1201 cccgaagtgg tggccaagaa cttggtcgag gcgctcgcgg gcgccggggc ggtccagagc
     1261 gccaagctgg atcgcaagac cggaaccctg accatggtgg tcaaagacgt cttgaccgac
     1321 acgggcaagc ccccggccca gaatcgggag cagctgtccc ggcaggggtc gctggaggtc
     1381 gagagcatcc tgggaatgat caccttcaag cagatcgtcc tccagatcgt gaaggatggg
     1441 aaggtcctgg ccaccgtccg gggcgagccc gggaagcctc cgcagacgga gttcgcgccc
     1501 gacctcaagt aacggccggg ccgtccctcc ttcctcagaa ggcgaacacc acccggacct
     1561 gcgcgctgat cgagagcgtt cccgggagca cgggggttgc gacctccggc gccgccctca
     1621 ggggttcccc acggatcggg acggttgcac ccagctcctc gatccgcacg atcctggggg
     1681 tggcgaggcc ggccgccgct gccagggcgt tggccgaggc gcgggcatcc tgaaccgcgg
     1741 cggcgaaggc ccgcgtgcgg gacgccgccg ggtcgtggac ggtaaaggcg acgccatcga
     1801 ggaggttggc cccggccgtg accgcggcat cgaggacgcg ccccaccagg ctgagatcgt
     1861 cgacggtgac cctggccctc tgcacggcct ggtacccgac gatctcgccg gccgggggcc
     1921 gccgctgcgg aaacaggttg atctcgaccg tctggatgcg ctcgcgcggg atcccgaggg
     1981 ccatcacctg ccggagcgtc tgggtcatcg tcatgctcgc aacctcctga gcctcctgcg
     2041 cggtcgcgcg cgtgaactgc gctcccaccg tgacgatcgc ccggtctgga gcggcctcga
     2101 caaccccacg cccggtgacg atgatccgct ggtccggaga ggtcgggggg gaatcggcgg
     2161 gagtcgaagg aggagccgca gttgatgcgg gcgtgcctgc ggcggcatac cccgcaagcg
     2221 cgaccgcggc gatgagccct gccgcaacca tccgtgccct ggccatgaac gcacctccgg
     2281 aagatgggct gttcctgaat acgggaatcc cccggggcat gttccccccg gaccaagacg
     2341 ggggcgcccg gggggtgtgg agggaagccc ggccggaaac acgaaacaaa cgccagatgt
     2401 ttgcgttgaa cttctactcg agcgtcttca cgcaggccct ccgtgacggc cgcaagaccc
     2461 tcacgatccg cctgggagac aagcgcgaca agtaccaggc aggccagctg gtctgggtca
     2521 cggtcgggaa ccggttcggc gccaagcagc gcatcttcac cgccatgatc gaccgggtgg
     2581 aggtcaaggc gctgcgtgag gtcacccccc gggagatcga gcgggaaaac cccgcgacgc
     2641 gggagcacac cgatctgata gagtttctgt ccaagatcta cgcgcgcaag atcggggcgg
     2701 aggacacggt gaccgtgatc cacttttcac gggtcaccga ggcgaaggac gaataaccgg
     2761 ctcccgctgc gccggccgtg acgacgcggg ggtcctccgt cgtgccgccc tccgtcattg
     2821 aatgcgttcc caacttcagc gaaggacggc ggctcccggt gctggacgcg ctggccgcca
     2881 gcattcgggc cgtggacgcg gtgcggctgc tggacgtctc gtccgacccc gaccacaacc
     2941 gggcggtctt cacgttcgtc ggcgacggtg agcgcgtcct cgaggcggcg ttcagaagcg
     3001 cggtggtggc catcgagcgg atcgatctcc ggacgcaccg gggcgtccac ccccgcatcg
     3061 gcgccgtcga tgtcatcccg ttcgtgccac tgcttaacgc gacgatggac gcctgcgtgg
     3121 cgctggcccg tcgcctgggc gcgcggctgg cagccgatct cgaccttccc gtgtacctgt
     3181 acggccgcgc gggtccccag ccctcgcggt cgctcgcgga gatccggcgg ggaggattcg
     3241 aggggttgcg gatggcgatc gcccaaccct cccgggctcc ggagctcggg ccggcccggg
     3301 tgcatccgac gggtggcgcc gtggccgtcg gcgcgagaga gattttgatt gcgttcaacg
     3361 tggaattgca gagcaccgat gtgaaggtcg cccggacgat tgcgcgcgcg gtgcgcgagt
     3421 cctcgggcgg acttcccgcg gtccaggcga tgggggtccc gctcgcctcc cggggatgcg
     3481 tgcaggtctc gatgaacctc caggattacc gccgcacgcc cccgcttgtg gccttccagc
     3541 gggtcgaggc ggaagccgcc cgccgcggca tcgcggtctc cgccggtgaa ctggtcggct
     3601 gcgcgccccc cgaggccctg ccgccggatc ccgtggccgc cctgcgcctt cggagcctcc
     3661 gcccggggca gatccttgat ccggcgaggc tcgcgcgcga cctcgacggt gggggtgc
//
LOCUS       DASQGH010000156         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_678, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000156 DASQGH010000000
VERSION     DASQGH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VEW91_02935"
     CDS             complement(<1..547)
                     /locus_tag="VEW91_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HYM90574.1"
                     /translation="MSRVVLSGYYGFDNLGDEAVLAATVAALRARRPGVEIAVLSGSP
                     EATGRAFSVEGVPRSRLGALVRVLRGCDLFLSGGGSLFQDVTSWRSPWYYLGVLALAR
                     RLGCRTAIYAQGIGPLRGRAIRSAARRQLSAVDLITLRDRESLSALAALGVDRPPAVL
                     TGDPALLLTPEWSARVAAEMSG"
     gene            complement(561..2606)
                     /locus_tag="VEW91_02940"
     CDS             complement(561..2606)
                     /locus_tag="VEW91_02940"
                     /inference="COORDINATES: protein motif:HMM:NF039831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HYM90575.1"
                     /translation="MIPRLLWSRYPVLAVCVGAALVAALVVLVHRYRIEQSYRTVEIT
                     VDGDDWTLLARRTGADRDAFYNALHEAGARSVTVYAASLKRLADAGRVTFMTGADLLN
                     LARLGPVDGPFGALLRAGRVLPGNTYVAGALPVLDQVRSGLAVQLVPRGGSARLALVE
                     GSGPVLEIQARGPEIEDAPLGVFPEDSAVIRSHHLAVEARMRNFREVVPGGLEAFFAG
                     LRRVGERLTLIFDGTQVLGYEQLIPQVAEQMKASGFAYGQIETFTARRRQRGDLELVQ
                     SVAPSVLRVFSLTPEELAVLTPEDARDKYVLAARERNVRILYVRPFLNTSAGEDQIQA
                     NLNYIRSIGDALERAGYRMGKAAPLPNVGIPAWLFMVMALGALAAAAMAVGEVAAILA
                     RPVPARLLGAGVLAGGLGTLAVIAVRHVTLWSQLLALLAALAFPTLSLMGVLPRNGEP
                     RGRAHGPGARVVAGSVLAVWAVSAGSALGGVMVAALLSQWAFMLEIKEFLGVKLAHIV
                     PVVLIGLMLAAADAPRGELWPRLRAWARQPLLLEYGIIVIAVGLAAVFALGRTGNAGL
                     PALGALELKSRVILSHLVVARPRTKEYLIGHPFMVLAFALSSFGLRRWALPAALVGAI
                     GQVGLVNSFSHIHTPLVYVILRTLYALVFGSAIGAVLVGLLLWSRRWWSPQGRPVRA"
     gene            complement(2622..3332)
                     /locus_tag="VEW91_02945"
     CDS             complement(2622..3332)
                     /locus_tag="VEW91_02945"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HYM90576.1"
                     /translation="MPRADWSRRLWWSAVGILAAAVGVNLATPVVAAALVGEPLRTYL
                     DAAGVDVTLEAWPPPALWWGRMDRITVAARDVRAGAQRLERFSATLGGVRVDPLALYL
                     ERAFVVRAIGSGHAQATISEAALARTLARDSGVRISALMLRPGRVLVRGAIRVLDTDV
                     AVAGAGRLILSRGGTIDLLLDRALVTGATDPAAFKGQLTTRIPAVVRIPPLPFGLQLT
                     GVRVDDGRLVLDAGLGSS"
BASE COUNT          581 a         1302 c         1230 g          383 t
ORIGIN      
        1 accccgacat ctccgccgcc accctcgccg accactccgg ggtcaggagc agggccggat
       61 cgccggtgag cacggcaggg ggacggtcca ccccgagcgc ggcgagggcc gagagggact
      121 cccgatcccg cagcgtgatc agatcgacgg cgctgagctg ccgacgcgcg gcggatcgta
      181 tcgcgcggcc gcgcagcggc ccgatcccct gggcgtagat cgccgtgcgg cagcccagcc
      241 gccgggccaa cgcgagcacc ccgagatagt accacgggct tcgccagctc gtgacatcct
      301 ggaacaggct tccgcccccc gacaggaaaa ggtcgcaccc ccggaggacg cgcacgaggg
      361 ccccaaggcg ggagcgggga acgccctcga ccgagaacgc ccgaccggtc gcctctggcg
      421 agcccgagag cacggcgatc tcaacccccg ggcggcgggc ccgcagggcg gcgacggtcg
      481 ccgcgagcac cgcctcatct cccaggttgt cgaatccgta gtacccggag agcaccaccc
      541 ggctcatcgt cgggcggcgt ctaggcgcgg accggccgcc cctgggggga ccaccagcga
      601 cggctccaca gcaacagccc gacgagcacc gcgccgatgg ccgatccaaa gacgagcgcg
      661 tacagggtgc ggaggatgac gtagacgagc ggggtgtgga tgtgggagaa cgagttgacc
      721 aggccgacct ggccgatcgc gccgacgagc gccgcgggca gggcccagcg gcggagcccg
      781 aaggaggaca gcgcaaacgc cagcaccatg aacgggtgcc cgatcaggta ttccttcgtc
      841 cgggggcgcg cgacgacgag gtgcgacaag atcacccggc tcttgagttc gagcgcgccc
      901 agcgccggca gcccggcgtt gccggtccgc ccgagggcga aaaccgcggc gagccccacc
      961 gcgatcacga tgatgccgta ctcaagcagg agtggctgtc gcgcccacgc ccgaaggcgc
     1021 ggccacagct cgccgcgagg cgcatccgcg gcggcgagca tcaatccgat caggaccacc
     1081 gggacgatgt gggccagctt gaccccgagg aactccttga tctccagcat gaaggcccac
     1141 tggctgagaa gcgcggcgac catgacgccg ccgagcgcgc tgccggccga gaccgcccac
     1201 accgccagga cgctgcccgc caccacccgg gcccccgggc catgcgcgcg cccgcggggc
     1261 tcgccgtttc ggggcaggac ccccatcagg gaaagcgtcg gaaacgccag cgcggcgagc
     1321 agcgccagga gctgactcca cagcgtcacg tggcgcacgg cgatcactgc gagggtgccg
     1381 aggccgccgg ccaggacccc tgcgccgagc agtcgagcag ggaccggccg ggcgaggatc
     1441 gcggccacct cccccacggc catcgccgcg gccgccagcg ccccgagggc catgaccatg
     1501 aacagccagg ccgggatccc cacgttcggg aggggcgcgg ccttccccat ccgatacccc
     1561 gcccgctcga gggcgtcccc gatcgagcgg atgtagttca ggttcgcctg gatctggtcc
     1621 tcgccggccg aggtgttcag gaacggccgg acgtagagga tgcgcacgtt ccgctcccgc
     1681 gccgcgagca cgtacttgtc gcgcgcgtct tccggcgtga ggacggccag ctcctccggg
     1741 gtgaggctga acacccggag cacagagggc gcgacgctct ggacgagctc gaggtcaccc
     1801 cgctgccgcc ggcgggcggt gaaggtctcg atctgcccgt acgcgaaccc ggatgccttc
     1861 atctgctccg caacctgggg aatgagctgc tcgtatccga ggacctgggt gccgtcaaag
     1921 atcagggtca accgctcccc cacgcgccgg agccccgcaa agaaggcctc cagcccgccc
     1981 gggacgacct cacgaaagtt ccgcatgcgc gcttccacgg caaggtggtg cgagcggatc
     2041 acggcactgt cctcggggaa caccccgagc ggagcgtcct cgatctccgg accccgcgcc
     2101 tggatctcca gcaccggtcc gctgccctcc accagcgcga gccgggccga tcccccgcgg
     2161 ggcaccagct gcaccgcgag cccggatcgc acctggtcga gcaccgggag ggccccggcc
     2221 acgtaggtgt tgcccggaag gacgcggccg gcccgcagca gggcgccaaa cggtccgtcc
     2281 accggaccca gccgcgcgag gttcaggagg tcggccccgg tcatgaacgt cacgcgcccg
     2341 gcatccgcca agcgcttgag cgaagccgcg tagaccgtga cgctccgggc ccctgcctcg
     2401 tgcagcgcgt tatagaaggc gtcccgatcg gccccggtgc gccgggccag cagcgtccag
     2461 tcgtcgccgt ccaccgtgat ctcgacggtg cggtaggact gctcgatccg gtagcggtgg
     2521 acgaggacca ccagggccgc caccaacgcc gcacccacgc acaccgcaag gacggggtac
     2581 cgggaccaga gcagccgcgg gatcaccagc gcctccgcgc gctacgacga gcccagcccg
     2641 gcatcgagca cgagccgccc atcgtccacg cgcaccccgg tgagctggag cccgaagggc
     2701 aagggcggga tccgcaccac ggcggggatg cgggtggtca gctggccctt gaacgccgcc
     2761 ggatcggtcg cgccggtgac gagggcccga tccaggagca ggtcgatcgt tccgcctcga
     2821 cttaagatga ggcggcctgc gcccgcgacg gcgacatcgg tatcgagcac acggatcgct
     2881 ccccggacga gcacccggcc gggccgcagc atcagcgcgc tgatccgtac ccccgaatcg
     2941 cgggccaggg tccgggcaag ggccgcttcc gagatcgtcg cctgggcgtg gccggacccg
     3001 atggcgcgaa cgacgaaggc gcgctccagg tacaacgcca ggggatcgac ccggacgccg
     3061 cccagcgtgg cgctgaagcg ctcgaggcgt tgggctcccg cccggacgtc ccgggccgcc
     3121 accgtgatcc gatccatcct cccccaccac agcgcggggg gcggccacgc ctcgagcgtg
     3181 acgtcgaccc cggcggcgtc aaggtaggtg cgcagcggtt ccccgacgag ggcggcggcg
     3241 acgaccgggg tggcgagatt caccccgacg gcggcggcga ggatgccgac cgcagaccac
     3301 cacagccgtc tcgaccagtc cgcgcggggc acggctagga tgccgcctcc ggtcccctcg
     3361 aggccccggc ggggctcggc ggcttcccga ggcgcagcgc ggcatcgatg aatgcgtcca
     3421 ggcccccatc gaggaccgcc tgggcgtttc cggtctcgag acctgtgcga tggtccttca
     3481 ccagggtata ggggtg
//
LOCUS       DASQGH010000157         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_29097, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000157 DASQGH010000000
VERSION     DASQGH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29097"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VEW91_02950"
     CDS             <1..1098
                     /locus_tag="VEW91_02950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYM90577.1"
                     /translation="GKLSHHAFTEFEIPQLLAINAVSAAVFHSPGGVSVRVRRATVDS
                     YVVDRVAFDRWLVARAASAGAEVMTGVRMTAVRRSSGGMLVSGVRSGRPFEASCRLLI
                     DAEGAAPRLPGSLGLRFPRRYAIGLQYTMRGVGGIEPDTPEVFLGGGVAPGFFAWLMP
                     VGPGRARVGLCVDPRVTRRAPVWYLERLIATHPALRHRLAGARVDEKVAGRIALLAPG
                     RPAHADGLMIVGDAAGQVKATSGGGIYYAMIAGRIAGEAAPRYVGGDRRAAAAYERRW
                     RARFGREVAFTAFGRRAINRLSDANLDAFLRAIAESPQIRGRVERAGDTQFQSRLFLP
                     LLRGVCGAGLRQPALIPVVIKALLGGMLAQL"
     gene            1147..2394
                     /locus_tag="VEW91_02955"
     CDS             1147..2394
                     /locus_tag="VEW91_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HYM90578.1"
                     /translation="MRIIADFHLHSKFSRATSREMDVETLARWCGLKGITVVGTGDFT
                     HPVWLRELRGKLRASGRGLYTCGPQHFILSTEVSNIYPQGGRLRKIHNVLLAPSFEVV
                     DRINAVLSRFGSLMADGRPTLSLPSDKLVEYVMEVSPECMVIPAHVWTPWFSLYGSNS
                     GFDSLRECFGDQARHIHAVETGLSSDPPMNWRIGELDSVMLVSNSDAHSAPKLGREAN
                     VFDCALDYGEILGALRRRDRSKFLFTIEFFPEEGKYHFDGHRACNQRLSPKETRAARG
                     ICPACGKPLTVGVMSRVEALATREDGAGPGRVPYRHLIPLEEIIAEALGTQPGASAVR
                     EEYLGLTSALTSEFAILMDVPLDEVARHTRPRVVEGLRRVREGRVQIRPGYDGVFGEI
                     RIFGGAAEERRGEEQQPAQTSLF"
     gene            2460..>3218
                     /locus_tag="VEW91_02960"
     CDS             2460..>3218
                     /locus_tag="VEW91_02960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYM90579.1"
                     /translation="MRPGGARRLLTGHLWVYRTEIARLDGDPKDGDVVAVRTVPGRHL
                     GMGLLNTRSAIAVRLLAAEEREIDERFFADRLRSALALRARLVPPGTACRLVYSEGDY
                     LPGLIVDRYGDLLVVQTLTLGMDQRKELIVRLLMEMAGPRGIYARNDPAVRRLEGLPR
                     ETGWLAGGGPTHVEIGEGDCRFVVDIAQGQKTGFFLDQRENRTRVADLAPGARILDGF
                     AYTGAWGIRAVRRGGGVVTGLEISEAAVALAKQNA"
BASE COUNT          442 a         1080 c         1202 g          494 t
ORIGIN      
        1 gggaaactct cgcaccacgc cttcacagag ttcgagatcc cccaactgct ggcgatcaat
       61 gcggtgagcg cggcggtgtt tcactcgccc gggggcgtct ccgtccgggt ccgccgggcc
      121 acggtggatt cctacgtcgt cgatcgggtg gcgtttgacc gctggctggt ggcccgcgcc
      181 gcgtccgcgg gagcggaggt gatgaccggc gtccgcatga ctgcggtgcg gcggagctcc
      241 gggggcatgc tcgtgtccgg ggtgcggagc ggccggccgt ttgaggcctc gtgccgcctg
      301 ctcatcgacg ccgagggcgc cgccccccgg ctgcccgggt cgctgggtct ccggttcccg
      361 cgccggtacg cgataggcct gcagtacacc atgcgggggg tgggcggcat cgagccggac
      421 accccggagg tgtttctcgg cgggggggtg gccccggggt tcttcgcctg gctgatgccg
      481 gtgggaccgg gccgcgcccg ggtgggtttg tgcgtggatc ctcgggtcac ccggcgcgcc
      541 ccggtctggt acctcgagcg gctgatcgcg acccaccccg cgcttcgcca ccggctggcc
      601 ggggccaggg tggacgagaa ggtcgccggt cggatcgcgc tgctcgcccc cggccgtccg
      661 gcccacgccg acggcctcat gatcgtcggc gacgccgccg gtcaggtgaa ggcgacgtcc
      721 ggcgggggca tctactacgc gatgatcgcc ggccgcatcg ccggcgaggc ggcgccgcgc
      781 tacgtcggcg gtgaccggcg cgccgcagcg gcgtacgagc gacggtggcg ggcacggttc
      841 ggccgcgagg tcgcgttcac cgccttcggc cggcgggcga tcaaccgcct gtcggatgcc
      901 aacctcgacg cgtttctccg ggcgatcgcg gagagcccgc agattcgagg ccgtgtggaa
      961 cgggccggcg acacccagtt ccagtcccgg ctctttctgc cgctcctgcg cggcgtgtgc
     1021 ggggcggggt tgagacagcc cgcgctcatt ccggtggtca tcaaagcgct gctgggtggg
     1081 atgctggcgc aactctgacg cggccggcgg gggaggataa cccccccaag acacgaatcc
     1141 actcccatgc ggatcatcgc tgacttccac ctccactcca agttcagccg cgcaacaagc
     1201 cgcgagatgg acgtcgaaac cctcgcccgg tggtgcggcc tcaagggcat caccgtggtg
     1261 ggcaccgggg acttcaccca cccggtgtgg ctgcgcgaac tccgggggaa gctccgcgcc
     1321 agcgggcggg ggctgtatac ctgcggaccg cagcatttca tcctcagcac cgaggtgagc
     1381 aacatctatc cccagggggg ccgattgcgg aagatccaca acgtcctgct cgcccccagc
     1441 ttcgaggtcg tcgaccgcat caacgccgtg ctcagccgtt tcgggagcct catggccgat
     1501 gggcggccca cgctctcgct gccgagcgac aagctggtgg aatacgtgat ggaggtctcg
     1561 ccggagtgca tggtgatccc cgcccacgtc tggacgccgt ggttctccct ctacgggagc
     1621 aactccgggt tcgactcgct ccgggagtgc ttcggcgatc aggcgcgcca catccacgcg
     1681 gtggagaccg ggctcagcag cgatcccccg atgaactggc ggatcggcga actggactcg
     1741 gtgatgctgg tgagcaactc ggatgcccac tcggccccga agctggggcg cgaggcgaac
     1801 gtgttcgact gcgcgctcga ctatggagag atcctcggcg cgctccggcg gcgcgaccga
     1861 tcgaagttcc tgttcaccat cgagttcttc cccgaggaag ggaagtatca cttcgacgga
     1921 caccgggcgt gcaatcagcg gctgtccccg aaggagaccc gcgccgcccg cggcatctgc
     1981 ccggcgtgcg ggaagccgct gacggtgggg gtgatgtcgc gcgtcgaggc gctcgcgacc
     2041 cgcgaggatg gcgcgggccc cggccgggtg ccgtaccgcc acctgatccc gctggaggag
     2101 atcatcgcgg aagcgttggg gacgcagccc ggcgcgagcg cggtgcgcga ggagtatctg
     2161 gggctcacct cggcgctcac gagcgagttc gcgatcctca tggatgttcc gctcgatgag
     2221 gtcgcccggc acacccggcc ccgggtcgtg gaagggttgc ggcgggtgcg cgagggacgg
     2281 gtccagattc gccccgggta cgacggggtc ttcggcgaga tccgcatctt cggcggagcc
     2341 gcagaggaac gccgggggga ggaacaacaa ccggcgcaga ccagcctgtt ctgatcccga
     2401 gccgggggcg ctcgcgccgg gccggccgga ccggggccgc atcgcggccg gtggtggttg
     2461 tgcgtcccgg tggtgccagg cggctcctga ccggtcacct gtgggtctat cggaccgaga
     2521 tcgcccggct cgatggcgat cccaaggacg gcgatgtggt ggcggtgcgg accgtacccg
     2581 gccgccacct cgggatgggg ttgctcaaca cccgctcggc gatcgcggtg cggctgcttg
     2641 ccgccgaaga gcgcgagatc gacgaacggt tcttcgcgga ccgcctgcgg agcgccctcg
     2701 ccctccgcgc gcgcctggtc ccgccgggca ccgcctgccg gctcgtgtac agcgaagggg
     2761 actatctgcc cgggttgatc gtcgatcggt acggggatct cctcgtcgtc cagaccctca
     2821 ccctcggcat ggaccagcgg aaggagctga tcgtccgcct cctcatggag atggcggggc
     2881 cgcgggggat ctacgcccga aatgatccgg cggtgcgccg gctggagggg ttaccccggg
     2941 agacgggctg gctcgccggg ggcggaccga cccatgtgga gatcggggaa ggcgactgcc
     3001 ggttcgtggt ggatatcgcg caggggcaaa agacggggtt ctttctcgac caacgcgaga
     3061 atcggacgcg ggttgccgac cttgcccccg gcgcccggat cctggacggc ttcgcctaca
     3121 cgggggcctg gggcatccgg gccgtccggc gcgggggagg ggtcgtgacc ggcctggaga
     3181 tctcggaggc cgcggtcgcg ctggcgaagc agaacgcg
//
LOCUS       DASQGH010000158         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_26159, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000158 DASQGH010000000
VERSION     DASQGH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26159"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VEW91_02965"
     CDS             <1..771
                     /locus_tag="VEW91_02965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYM90580.1"
                     /translation="GRARLAAEALDTTLPGRRTATGRTHILTRTMEEIAEIFRGLGFE
                     VVEGPEIESDHDNFERLNMPAYHPARDAQDSFYVAKEWLLRTHTTVVDVHVLDARRPP
                     MRALSYGHCYRRDAADASHSPMFHQADGFMVDEGVRFSDLKGVLLAFAHAFFGPQIRT
                     RFTPSYFPFTEPSAEMAISCVICQGRGCAVCKRSGWLEILGCGMFHPRVLEMAQIDPE
                     RYTAFAFGMGVERPAMLKHRISDIRLFYENDVRFLGRV"
     gene            774..2825
                     /gene="pheT"
                     /locus_tag="VEW91_02970"
     CDS             774..2825
                     /gene="pheT"
                     /locus_tag="VEW91_02970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYM90581.1"
                     /translation="MRLSFDWLREYVDIDVPLEALVERLPSIGLPVEQVERVGNDAVL
                     EIDLTSNRPDCMSILGIAREVALLLGRPLRVPVSKPVEHAPAASTRVAVAVADPEGCP
                     RFTARVIEEVRIGPSPAWAQRRLEASGIRAINNVVDATNYVMLELGQPMHAFDYDRIA
                     GHRLVVRRAEPGERLTTLDGVARTLDPEMLVVADARRAVALAGVIGGGETEINRSTTR
                     VLLEAAYWNPPAIGRTARRLGLRTEASARFERGMDPAGPPGAQRRAAQFLAEWCGGRV
                     LRGVVDVYPRTLGKRAIRLRSKRAVAMLGVDVPRTEMRRILRALGCRVTPGAILEVRP
                     PSFRPDLLREEDLIEEVARIYGYDRVPPTMPRGETTPGAIAPVLRIDARVRETLARSG
                     LTEVLTLTLVSRETGARGGAAPVVLQNPLTTDQAVLRTSLLPGLVAVLATNAARRAEN
                     VHAFELGRVFRAGAAGGRPEERRALGIAVMGRWRSGWNIPADQAVADFFHLKGIIEGL
                     LEELGVGGVAVGPSPDAAAGWWHPGRAAALVSGGTTIGRLGELHPDLATAHRLPSRAY
                     LAEVDLEALLPVVALERASPDLPRYPAVERDVAAVIPEGLPAGQVEAAIRAAAGPLLE
                     AVELFDVYTGPPVPAAHRNLAYRLRLRAPDRTLTAEEAEEIVQQVRIALEEQVGVRLR
                     E"
     gene            2851..>3050
                     /locus_tag="VEW91_02975"
     CDS             2851..>3050
                     /locus_tag="VEW91_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HYM90582.1"
                     /translation="MGRTITVNWIDWITLAIILVAILRGTRYGVLAGVIDLAALGGAF
                     LIASILYGHVVPGLHEALNLPN"
BASE COUNT          411 a         1042 c         1117 g          480 t
ORIGIN      
        1 gggcgcgccc ggctggcggc cgaggcgctc gacaccaccc tccccgggcg ccgcacggcg
       61 accggccgga cgcacatttt gactcggacg atggaggaga tcgcggagat cttccgcggc
      121 ctggggttcg aggtggtgga ggggccggag atcgaatccg accacgacaa cttcgaacgc
      181 ctcaacatgc ccgcctacca ccccgcccgg gatgcccagg attcgttcta cgtcgcgaag
      241 gagtggttgc tgcggaccca caccaccgtg gtggatgtgc acgtcctcga cgcccgccgc
      301 ccgccgatgc gggcgctgtc gtacggccac tgctaccgca gggacgcggc ggacgcgagc
      361 cactcgccga tgttccacca ggcggacggg ttcatggtcg atgaaggggt gcggttctcc
      421 gacctcaagg gggtcctcct ggcgtttgcc cacgcgttct tcgggccgca gatccggacc
      481 cgcttcaccc cctcctactt cccgttcacg gagccgagcg ccgagatggc gatctcgtgc
      541 gtgatctgcc aagggcgcgg gtgtgcggtg tgcaagcgct cgggctggct ggagatcctc
      601 gggtgcggca tgttccaccc ccgcgtcctc gagatggcgc agatcgaccc cgaacggtac
      661 accgcgttcg cgttcggcat gggggtcgag cgccccgcga tgctcaagca ccgcatcagc
      721 gatatccggt tgttctatga aaacgatgtc cggtttctcg gccgggtcta gcgatgcgcc
      781 tctcctttga ctggctgcgg gagtacgtgg acatcgacgt tcccctggag gcgctggtcg
      841 aacggcttcc cagtatcggg ctgcctgtcg agcaggtcga gcgcgtaggg aacgacgccg
      901 tcctcgagat cgacctgacc agcaaccgcc ccgactgcat gagcatcctg gggattgccc
      961 gggaggtcgc cctcctcctc ggccggcccc tccgggtccc cgtctcgaag ccggtcgagc
     1021 atgcgcccgc cgcctcgacg cgggtggcgg tcgcggtcgc cgatcccgag gggtgccccc
     1081 gcttcacggc gcgggtcatc gaggaggtcc ggatcggccc gtcgccggcg tgggcgcagc
     1141 ggcgcctcga agcctcgggg atccgggcga tcaacaacgt cgtcgatgcc acgaactacg
     1201 tcatgctcga actcggccag cccatgcacg cgttcgacta cgatcggatt gccgggcacc
     1261 gcttggtcgt gcggcgcgcc gagcccggag agcggctcac cacgctggat ggggtcgccc
     1321 ggacgctcga cccggagatg ctggtcgtcg ccgacgcccg gcgcgccgtc gcgctggcgg
     1381 gcgtcatcgg cgggggcgag acggagatca atcgcagcac gacgcgggtg ctcctcgagg
     1441 cggcctactg gaatccgccg gcgatcggac ggacggcgcg ccggctcggc ctgcggacgg
     1501 aggcctcggc gcggttcgaa cggggcatgg accccgccgg cccgcctggc gcgcagcggc
     1561 gagccgcgca gttcctcgcc gaatggtgcg gcggtcgagt gctgcggggg gtcgtcgacg
     1621 tgtacccccg cacgctcggg aaacgggcga tccgactgcg gtccaagcgg gccgtggcga
     1681 tgttgggggt ggacgtgccg cgaacggaga tgcgccgcat cctccgcgcg ctggggtgcc
     1741 gggtgacccc cggggcgatc cttgaggtgc gcccgccctc gtttcgcccg gatctcctgc
     1801 gcgaggagga tctgatcgag gaggtggccc ggatctatgg gtacgatcgg gtcccgccga
     1861 cgatgccccg gggggagacc acgcccggcg ccatcgcgcc cgtccttcgg atcgatgcga
     1921 gggtccggga gacgctcgcc cgatccgggc tcaccgaggt ccttaccctg acgctcgtct
     1981 cccgggagac cggcgcacgg ggcggggccg cgcctgtcgt tctgcagaat cccctaacta
     2041 ccgatcaggc ggtcctgcgg acctcgctcc tgcccggtct ggtggcggtc ctggcgacca
     2101 atgcggcgcg gcgcgcggag aacgtccacg cctttgagct cgggcgggta tttcgcgccg
     2161 gcgctgccgg cggccgcccg gaggaacgcc gggcgctcgg gatcgcggtg atggggcggt
     2221 ggcgaagcgg atggaacatc ccggccgatc aggccgtggc ggacttcttc catttaaagg
     2281 ggatcatcga agggctcctg gaggagttgg gggtgggcgg cgttgcggtg gggccttcgc
     2341 ccgatgctgc ggccgggtgg tggcatccgg ggcgcgcagc cgcgctcgtg tccggcggga
     2401 ccacgatcgg ccgcctcggc gagctgcacc ccgatctcgc caccgcgcac cggctgccgt
     2461 cccgcgccta tctggccgag gtggatctcg aggcgctgct ccccgtggtc gccctcgaga
     2521 gggcgtcccc ggatcttccc cgatacccgg cggtcgaacg ggacgtggcc gcggtcatcc
     2581 cggagggcct gccggcgggc caggtcgaag cggcgatccg ggccgccgcc ggcccattgt
     2641 tggaggcggt cgagttgttc gacgtgtaca ccggaccgcc ggtgcccgcg gcgcaccgga
     2701 acctcgcgta ccgcctgcgg ttgcgcgctc cggaccgcac gctcaccgcg gaggaggcgg
     2761 aggaaatcgt tcagcaggtt cgaatagccc tcgaggagca ggtcggcgtt cggctccgcg
     2821 agtgatgctc gcgggaggcg ctcagaaggg atgggccgga cgattaccgt gaactggatc
     2881 gattggatca ccctggcgat catcctcgtc gcaatcctcc gcgggacccg ctacggggtc
     2941 ctggccggcg tgatcgacct cgcagccctg ggcggggcgt tcctgatcgc ctcaattctg
     3001 tatgggcacg tcgtgccggg gctgcacgag gcgttgaatc tgccgaatga
//
LOCUS       DASQGH010000159         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3516, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000159 DASQGH010000000
VERSION     DASQGH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3516"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VEW91_02980"
     CDS             <1..953
                     /locus_tag="VEW91_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HYM90583.1"
                     /translation="QAHPDYYLRIVEERSDGIVVRGAKVHTSVSMNANELIVLPTRTM
                     GERDRAYAVAFAVPIATRGLTLVVSPYGGHAASAFEYPLSSRHRMTESLTVFDDVFVP
                     RDRVFLQGEWEHAGLLARTFVEFHRFTAVSYKLPLVDLLVGAAQLIAEYNGIERAGHV
                     RDKITWLAAYAGTLRALTRMAAIDCSREALEIAVPDPGTVNIAKWHFATQFHRAVACV
                     QEIAGGLLVTAPGEADWEQPATRPLLERYLGGKGVDAEARLRLLHLISDVTTSALGGY
                     HAVLAIHAEGSIEAEKLTIYRTAPLDRCVALAKELAGIAR"
     gene            complement(950..1270)
                     /locus_tag="VEW91_02985"
     CDS             complement(950..1270)
                     /locus_tag="VEW91_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HYM90584.1"
                     /translation="MAGGSAGTTRSHPQGQTRLYLIAWLVLVAITALEVSLILVFRVP
                     PAVRATALIILALMKASLIGAYYMNLRLERLAMVYVAVIPLLLLVLMLIAIVPDAARL
                     LRRP"
     gene            complement(1321..1944)
                     /locus_tag="VEW91_02990"
     CDS             complement(1321..1944)
                     /locus_tag="VEW91_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HYM90585.1"
                     /translation="MTGAVDARERGDLATRWSGGRSPWDVSWGKLMMWLFLISDAMTF
                     AALLIGLWVERLGSPTWPNRLEIINLRFVATMTVILIFSSTTMALAVYAIRQGARRQA
                     VWFLTATIAAGCVFAGMQATEWLHFIGEGARLTGNPWGVPAFSFTFFVITGLHGAHVL
                     GGVVYLWIMADRIARGLSSAEGVEIAGLYWGFVDLGWVFIWSSIYLL"
     gene            complement(1976..2632)
                     /locus_tag="VEW91_02995"
     CDS             complement(1976..2632)
                     /locus_tag="VEW91_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HYM90586.1"
                     /translation="MPTELLGKAKLAGTRSRGAEGGRDGFGRGEGAFPWGPVPALPAS
                     VAGMGLAFALAAIAMLFVSFTTTYLGHRQDGDWKAVPLPWILWLDTGVLLASSATVEW
                     ARRRLREGDAVGLRRGLTAAGALGLAFLAGQLLAWRQLADQGVYLASHPHSSFFYLLT
                     GVHGVHLLGGLAALGAILPGAWRGAYSPAGSTAVTLTATYWHFLTGLWLYVFVILFWL
                     "
BASE COUNT          464 a          988 c          938 g          384 t
ORIGIN      
        1 accaggcgca cccggattac tacctccgaa tcgtcgagga gcgctccgac gggattgtgg
       61 tccgcggcgc gaaggtgcac acgtccgtca gcatgaacgc caatgaactg atcgtgctcc
      121 cgacccgcac catgggggag cgcgatcggg cgtacgccgt ggcgttcgcg gtgccgatcg
      181 cgacgcgcgg gctgaccctg gtggtcagcc cctacggggg ccacgccgcc tccgcgttcg
      241 aatatccgct ctccagccgg caccggatga ccgaatcgct caccgtcttc gacgatgtct
      301 tcgtgccccg ggaccgggtg ttcttgcagg gggagtggga gcacgccggg ctcctcgcgc
      361 ggacgttcgt ggagtttcac cggttcacgg cggtgtccta caagctcccg ctcgtcgacc
      421 tcctggttgg ggcggcccag ctgatcgcgg agtacaacgg gatcgagcgg gccggccacg
      481 tccgcgacaa gatcacctgg ctggccgcgt acgccgggac ccttcgggcg cttacgcgga
      541 tggcggcgat cgactgctcc cgcgaggccc tcgagatcgc ggtgcccgat cccgggaccg
      601 tcaacatcgc caagtggcat tttgccaccc agttccaccg ggccgtcgcc tgcgtgcagg
      661 agatcgccgg ggggctcctg gtcacggcgc ccggcgaggc ggactgggag cagccggcga
      721 cccgcccgct cctcgagcgc tacctgggcg ggaagggggt cgacgccgag gcgcgcctcc
      781 ggctgctcca cctgatctcg gacgtcacga cgtcagccct cggcggctat cacgccgtgc
      841 tggcgatcca cgccgaggga tcgatcgagg cggagaagct gacgatctac cggaccgcgc
      901 ccctggaccg gtgcgtcgcg cttgcgaagg agctcgccgg catcgcgcgc tagggccgcc
      961 ggagcaaccg ggccgcgtca gggacgatgg cgatgagcat gaggaccagc agcagcagcg
     1021 ggatgaccgc aacgtagacc atggccagcc gctccaaccg gaggttcatg tagtacgcgc
     1081 cgatgagact ggccttcatg agcgcgagga tgatcagggc ggtggcgcgg accgcggggg
     1141 gcacgcggaa gaccaggatc aggctcacct cgagggcggt gatggcgacg aggacgagcc
     1201 aggcgatcag gtagagccgg gtctggccct gcgggtggct tctggtcgtg cccgcggatc
     1261 ctcctgccat agttgcgtgc ctccctgccc ggctgccccc cgtatggggg gtatgggggg
     1321 ttagaggagg tagatcgacg accagatgaa gacccacccc aggtcgacga acccccagta
     1381 gagccccgcg atctcgaccc cttccgccga ggagagcccc cgggcgatcc ggtcggccat
     1441 gatccacagg tagacgacgc cgccgagcac gtgggcgccg tgcagcccgg tgatgacaaa
     1501 gaaggtgaaa ctgaacgccg gcacgcccca cgggttgccg gtgaggcgcg ccccctcccc
     1561 gatgaagtgc aaccactccg tcgcctgcat gcccgcaaag acacacccgg ccgcgatggt
     1621 ggcggtgaga aaccagacgg cctgccgacg cgcgccctgc cggatcgcgt agaccgccag
     1681 ggccatcgtg gtgctgctga agatcaggat caccgtcatc gtggcgacaa accggaggtt
     1741 gatgatctcg agccggttgg gccaggtcgg gcttcccagc cgctccaccc acagcccgat
     1801 taagagcgcc gcgaacgtca tggcgtccga gatgaggaac agccacatca tcagcttgcc
     1861 ccagctgacg tcccacggcg accggccccc gctccagcgg gtcgccaggt ctcctcgctc
     1921 tcgtgcgtcc accgcgcctg tcatcgtctc ccgagtctcc tgtgaagggt ccagcctaca
     1981 gccagaacag gatcacgaac acgtacagcc acaacccggt caggaaatgc cagtacgtcg
     2041 ccgtcaacgt gactgctgtc gaccccgcgg gtgagtatgc cccacgccac gctcccggga
     2101 ggatcgcgcc gagcgccgcg aggccgccca gcaggtgcac gccgtgcacg cccgtgagca
     2161 ggtaaaagaa tgaactgtgg ggatggctcg cgaggtacac gccctggtcc gcgagttgcc
     2221 gccacgcgag caactgtccg gccaggaacg ccaatcccag ggccccggcc gccgtgaggc
     2281 cccgccggag cccgaccgcg tccccttccc tcagccgccg ccgcgcccac tccaccgtgg
     2341 cgctgctggc gagcagtacg ccggtgtcca gccagagaat ccacggcagg gggacggcct
     2401 tccagtcgcc gtcctgccgg tggccaaggt aggtcgtcgt aaatgacacg aacagcatgg
     2461 cgatcgcggc gagggcgaaa gccagaccca tgccggcgac gctcgccggg agggccggca
     2521 ccggccccca gggaaaggct ccctcccccc ggccgaaccc gtcgcgacca ccctccgcgc
     2581 cgcggcttcg ggtgcccgcc aacttcgcct tgcccagaag ttcggtcggc atggccggct
     2641 actgcccacc tccaccgtgg cccgcgtacg ccggggcggc cggcaccgtc tgggggagat
     2701 aatcctccgc cgctcccggc acgctgtagt cgtacggcca ccggtacacc accggcaggg
     2761 acggccagtt gccg
//
LOCUS       DASQGH010000160         8357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37570, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000160 DASQGH010000000
VERSION     DASQGH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37570"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..399)
                     /locus_tag="VEW91_03000"
     CDS             complement(25..399)
                     /locus_tag="VEW91_03000"
                     /inference="COORDINATES: protein motif:HMM:NF022519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlqD family protein"
                     /protein_id="HYM90587.1"
                     /translation="MASITLQRPVVIKAIVTEAFKRLYIADLEEAAKRVDAILQQIDV
                     QSRRVELERQVTPQTRNLRAQLELERQRQEATRIELQVRLREAQDLQLNTEFVQGTIE
                     GTVDVSVGDYLFDKISRTEIIV"
     gene            complement(410..643)
                     /locus_tag="VEW91_03005"
     CDS             complement(410..643)
                     /locus_tag="VEW91_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HYM90588.1"
                     /translation="MKALVEWVTRSLVDHPEAVEVREVDGPHATVLELRVAPDDLGKV
                     IGRGGRVIKAIRTLTRAAATRSGKRVTVEIVRG"
     gene            complement(640..918)
                     /gene="rpsP"
                     /locus_tag="VEW91_03010"
     CDS             complement(640..918)
                     /gene="rpsP"
                     /locus_tag="VEW91_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HYM90589.1"
                     /translation="MGVKIRLMRRGAKGQPFYRLVVADARSPRSGKYIEAVGSYNPRT
                     EPSTMKVNEEKVLAWLRKGARPSDAARVILEKTGVLQRWEEARAKGGR"
     gene            complement(956..2284)
                     /gene="ffh"
                     /locus_tag="VEW91_03015"
     CDS             complement(956..2284)
                     /gene="ffh"
                     /locus_tag="VEW91_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HYM90590.1"
                     /translation="MFDQLQGRLSEILTRLRGRGRLGVEDVDQALREVRLALLEADVN
                     FKVAREFVGRVREAAVGQEVWKSLTPGQQVIQIVSEELTRLLGAVHHPLRLAPKPPTV
                     ILLVGLHGTGKTTTAGKLALHLKKQGRRVLLAASDVRRPAAVTQLQVLGETAGIPVCA
                     PGPEADPVDVARRALEQARTTADVLIIDTAGRLHIDDALLAELKRIHEATTPHSTLLV
                     LDAMAGQDAARMAEGFHQAVPIDGLILTKLDGDARGGAALSVVATLERPIFFVGTGER
                     LEALEPFHPDRIASRILGMGDVLTLIERAQEQVTAEQARELERKLKRAEFTLEDFTKQ
                     LRQVRAMGPLDQLLGMIPGLGPRLRGLDVDERALGRIEAMINSMTPQERRQPQLIDGS
                     RRRRIARGSGTSVQEVNRLLRQFDEVKKMLRQFEAQGHRRGKFPMPFPPA"
     gene            complement(2277..2885)
                     /locus_tag="VEW91_03020"
     CDS             complement(2277..2885)
                     /locus_tag="VEW91_03020"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYM90591.1"
                     /translation="MIPQASHYFSPSPPAAAGKRTVELRLGDRVLRFRTAHGVFSRTS
                     VDRGTRLLLEAANLTGARRILDLGCGYGALGIAAAARAPQAQVVLVDVNPRAVALAGE
                     NIVLNRVPNAEARCGDGCAPVAGEQFDLILFNPPIRAGRDVVLRLLGEAHGCLRPGGR
                     LYLVARTQQGARTLGRLMGETYAWVREVARGGGFRVFEGCDV"
     gene            complement(2882..3271)
                     /locus_tag="VEW91_03025"
     CDS             complement(2882..3271)
                     /locus_tag="VEW91_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYM90592.1"
                     /translation="MPLHRIPRALDGRTAVVRLFDAYAGFLTARQRDMLRMYYHDDLS
                     LGEIAAGVGVTRQAVFDSLRRSVGDLRGLEKQVGVLAHRQRQARMRETAMKRLAAVER
                     EVARLAASGHGDLSPLRGALRALRAQL"
     gene            complement(3349..4269)
                     /gene="ftsY"
                     /locus_tag="VEW91_03030"
     CDS             complement(3349..4269)
                     /gene="ftsY"
                     /locus_tag="VEW91_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYM90593.1"
                     /translation="MASPPGWLERLRTGLARTRQSLTAQVDDLLQRGVREGFYEALEE
                     TLIQADLGVRTTDAVIARLRERARAGARTPDALRQALVEILTAALGAPAPLALTPPPA
                     VVLVLGTNGSGKTTTIGKLAWRLKGEGRKVLLVAADTFRAAAIDQLGVWADRVGVDVI
                     RHQPGGDPAAVAFDAAQAATARHMDVVIVDTAGRLHTKANLMEELKKVDRVLRRALPG
                     APVEALLVLDATTGQNGIAQARQFKAALPLTGIVLTKLDGTARGGVVVAIAEELGLPV
                     KLVGFGESLDDLQPFDPRIFAEALFAPEAG"
     gene            complement(4262..7894)
                     /gene="smc"
                     /locus_tag="VEW91_03035"
     CDS             complement(4262..7894)
                     /gene="smc"
                     /locus_tag="VEW91_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HYM90594.1"
                     /translation="MRLKRLELSGFKTFAERTVFEFSSQITGIVGPNGSGKSNVFDAI
                     RWALGEGSLRSLRGVRTEDIIFAGTDKRRPLAMAEVTVTLDNDDGSLVLPPDPDNGDA
                     PPTPLAFAEVTVTRRGMRNAESRYFINGLPCRLRDIQTMFLGTGLGGHSYALITQGEV
                     EQMLDATPDERRMILEEAAGLAKYKRRRHDAERRMAATETTLLRVTDILGELDAQTAQ
                     LATQAQAAEQYQAHTRELRTLELSIQVEEIRRGSRAQRRVRDQLEEIAARRREITSTV
                     QAVVEQRAALDRRAADAGREWDQFQQDRVSLAQRRAAAEAAVELVAERLRAVDSHRQR
                     TDREVSRARAEQEALRTERGAHDEAERDLATRQDEARAALEAHEVDLAGMDAAAARGE
                     EELEACRVAARALTEARAKADTEVAAAEAREAEHRDRSAGLDRRGAYLREQADAARAQ
                     QSQLGKESTEGMAGLEEARRRLVQLRAELDEHRAGQDALLADERRLEIEREGIASRLR
                     FLEEAQAQYRGYETGAREILLAWRAHPARLPGLRGAVADFLTVPRDLQPAFETALGPA
                     LSALVVSSVEDARAALALLGGEPRGSVMFVPVSAVAAPELPVLPSAVAADPGTVGRAV
                     DRITIAGPHPDAIRAVLADVLIVADLDAALRARANGYRGRVVTLAGEMLAPQGFLLAG
                     RRLPEQGGILGRAEEMAEIPAGLERVERSIGELGRRRGALGARIHDLEGGIAAAEALL
                     ARQAEAVAECRRRLTLADAEVERFAQEIAHLAMEQAATDQAAASQHTLRDRLREEAAG
                     LGARLTEVEAQIGRIAAGLRDHAGARRAAASRITELRVTLTEVSGRREAVRARISEVD
                     RGIAQLAAQCDELDDELGRFQLEAEQLHAERTAHLSRCEAIEAEGARLEAAQAALEAE
                     RAGLAADLAASETRHREALDRTAALDEEMHRAEVRQAQIEAELGSARRRMEEEFGVPF
                     DRAVGAVPESVNRDEALGRIEALRGLIAALGPVNLLAIEEHRQVAERARGLRTQMEDV
                     QGTLTTLRALIAHLEEEIRGQFDRTYEAVNKEFADLFVRIFGGGRAGLERVPGFDGAE
                     PGIEIIAQPPGKKLRSLGALSGGERVMVALALVFAMLRVRPSPFCVFDEVEAALDEAN
                     TRKVAEIFRELSARTQMIIITHNKATMEASDALVGITMEEGGVSQMVSMRLVDPETLG
                     APQPVG"
     gene            complement(7914..>8357)
                     /locus_tag="VEW91_03040"
     CDS             complement(7914..>8357)
                     /locus_tag="VEW91_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYM90595.1"
                     /translation="PERRELEAQVQRLGLAERVVFAGEQDHAYVVEALYASDLFVFPS
                     QTETLGLAVLEAMAAGRAVVAVRGGAIPEIVRDGETGRLVPPDPAALRRAIADLLETP
                     ALRRGMGERGKAWAASRSQARVVEQLVGVYHEVIAAHNAPTHAPA"
BASE COUNT         1066 a         3191 c         2861 g         1239 t
ORIGIN      
        1 cctgatttgg agcacgatcc cgtcctacac gatgatctcg gtgcgggaga tcttgtcgaa
       61 caggtagtcc ccgaccgaca cgtccaccgt gccttcgatc gtcccctgga cgaactccgt
      121 gttgagctgg aggtcctggg cttcccgcag gcggacctgg agctcgatgc gggtcgcctc
      181 ctggcgctgc cgctcgagtt cgagttgggc tcggaggttg cgggtctggg gggtcacctg
      241 tcgttccagc tcgacgcggc gggactgcac gtcgatctgc tggagaatcg cgtcgacccg
      301 cttggccgcc tcctcgagat ccgcgatgta caggcgcttg aacgcctcgg tcacgatggc
      361 tttgatgacg accggccgct gcaacgtaat cgatgccatc tcgctcctcc tatcctcgga
      421 cgatctccac cgtcacccgc ttcccgctcc gggtggcggc ggcccgggtg agggtgcgga
      481 tcgccttgat cacccggccc ccccggccga tcaccttgcc cagatcgtcg ggggcgaccc
      541 gcaactcgag gacggtggcg tggggcccgt ccacctcgcg gacctccaca gcctcggggt
      601 ggtcgaccag ggaccgggtg acccactcga cgagggcttt caccggccgc ccttcgcccg
      661 ggcttcctcc caccgctgga ggaccccggt cttttccagg atgacccggg ctgcgtcgga
      721 gggccgggct cccttgcgga gccaggccag gaccttctcc tcgttgacct tcatcgtcga
      781 cggctcggtg cgcgggttgt acgacccgac cgcctcgatg tacttgccgc tccgcgggct
      841 gcgggcatca gccaccacca accggtagaa cggctgaccc ttcgcgcccc ggcgcatcaa
      901 ccggatctta acgcccatcc acactcctcc tcacccgtga tccccgcgac gtgcctcatg
      961 cggggggaaa cggcattggg aacttgcccc tgcggtgccc ttgggcctcg aactgccgca
     1021 gcatcttctt cacttcgtcg aactggcgga gcagccggtt cacctcctgg accgaggtcc
     1081 cgctcccccg ggcgatccgc cggcggcgac tcccatcgat gagctgcggt tgccgccgct
     1141 cctggggcgt catggagttg atcatcgctt cgatgcgccc gagcgcgcgt tcgtccacgt
     1201 ccagtccgcg taaccgcggc cccagcccgg ggatcatccc gagcagctgg tcgaggggcc
     1261 ccatcgcccg cacctgccgg agctgcttcg tgaagtcttc caacgtgaac tcggcgcgct
     1321 tgagcttccg ctccagctcg cgggcctgct cggccgtcac ctgttcctgc gcccgctcga
     1381 tcagcgtcag cacgtccccc atgccgagga tgcgcgaggc gatccgatcg gggtggaacg
     1441 gctcgagcgc ctcgagccgc tcgcccgtcc cgacgaagaa gatcggccgc tcgagcgtcg
     1501 cgacgacgga gagcgcggct ccgccccgcg cgtccccgtc cagcttggtc aggatcagcc
     1561 cgtcgatcgg caccgcctgg tggaacccct ccgccatccg cgccgcgtcc tgcccggcca
     1621 tggcatcgag caccaagagc gtgctgtgcg gggtggtggc ctcgtggatc cgcttcagtt
     1681 cggccagcag cgcgtcgtcg atgtgcagcc ggccggcggt gtcgatgatc agcacgtcgg
     1741 cggtcgtgcg ggcctgctcg agcgcccgcc gcgccacatc gaccgggtcc gcctccggcc
     1801 ccggcgcgca caccgggatc ccggccgtct cgccaaggac ctgcagctgg gtgaccgcgg
     1861 cggggcggcg gacgtcggag gcggccagca gcacgcgccg cccctgcttc ttgaggtgga
     1921 gcgcgagctt gccggcggtc gtcgtcttcc cggtcccgtg cagcccgacc agcaggatca
     1981 ccgtcggcgg cttcggggcg agccgcagcg gatggtggac cgcgcccagc aggcgggtaa
     2041 gttcctcgga cacgatctgg atgacctgct ggcccggggt gaggctcttc cagacctcct
     2101 ggccgaccgc ggcctcgcgc acgcgtccga cgaactcccg ggcgaccttg aaattgacat
     2161 ccgcttcgag cagggccagc cggacttccc ggagggcctg gtccacatcc tcgaccccaa
     2221 gccgcccccg ccccctgagg cgggtgagga tctcggagag gcggccctgg agctggtcaa
     2281 acatcgcatc cctcgaacac gcggaagccg ccgccgcggg cgacttcacg cacccaggcg
     2341 taggtctcgc ccatcagccg gccgagcgtg cgggcgccct gttgggttcg ggcgacgagg
     2401 tacagccgcc cgccgggccg gaggcatccg tgcgcctccc cgagcaaccg cagcacgacg
     2461 tcgcgcccgg cccggatggg cgggttgaag aggatgagat cgaactgctc ccccgcgacc
     2521 ggcgcgcacc catccccgca ccgcgcctcc gcgttgggca cccggttcag cacgatattc
     2581 tcccccgcca gcgcgaccgc ccgcggattg acgtccacga gcaccacctg ggcctgcggg
     2641 gctcgcgccg ccgccgcgat cccgagcgcc ccgtagccgc accccaggtc gaggatgcgc
     2701 cgcgcccccg tgaggttcgc ggcctcgagc agcaggcgcg tgccccggtc cacggacgtc
     2761 cgcgagaaca ccccgtgcgc ggtgcgaaac ctgaggacgc ggtcccccag gcggagctcg
     2821 accgtgcgct tccccgccgc ggccggggga ctcggggaga agtagtgcga cgcctgcggg
     2881 atcacagctg tgcccgcagg gcccgcaggg cgccccggag cgggctgaga tccccgtggc
     2941 cggacgcggc gaggcgggcg acctcgcgtt cgaccgccgc cagccgcttc atcgccgtct
     3001 cgcgcatccg ggcctgccgc tggcgatggg ccagcacccc gacctgtttc tcgagccccc
     3061 ggaggtcgcc cacgctccgc cgcaggctgt cgaacaccgc ctggcgggtc acgccgaccc
     3121 cggcggcgat ctcccccagg gagaggtcgt cgtggtagta catgcgcagc atgtcccgct
     3181 ggcgcgcggt gagaaacccc gcgtaggcgt cgaacaggcg caccaccgcg gtccggccgt
     3241 cgagcgcacg cggaatcctg tgtaaaggca tttcacttca caggatagca cacgggccga
     3301 tcgggtgtaa acggcaccgg ggcgcgcggc gcgccgcctg ggggcgcctc accccgcctc
     3361 gggggcgaac agcgcctcgg cgaagatccg cggatcgaac ggctgcaggt cgtccagcga
     3421 ttccccgaac ccgacgagtt tgaccgggag gccgagctcc tcggcaatcg ccaccaccac
     3481 gccgccgcgg gccgtgccgt cgagcttcgt caggacgatc ccggtcagcg gcagcgccgc
     3541 cttgaactgc cgggcctggg cgatgccgtt ctgcccggtc gtggcgtcga gcaccagcag
     3601 ggcctcgacg ggcgcgcccg gcagggcccg gcgcaggacg cgatcgacct tcttgagctc
     3661 ctccatgagg ttggccttgg tgtggagccg ccccgcggta tccacgatca ccacgtccat
     3721 gtgcctggcg gtggccgcct gcgccgcgtc gaacgccacg gccgcaggat ctccccccgg
     3781 ctggtggcga atcacgtcca cgccgacccg gtccgcccac accccgagtt ggtcgatcgc
     3841 cgcggcgcgg aacgtgtcgg ccgcgacgag caggaccttc cggccctcgc ccttcaagcg
     3901 ccaggcgagc ttcccgatcg tcgtcgtctt gcccgatccg ttggtgccga ggaccaggac
     3961 caccgccggc gggggggtga gcgcgagcgg ggccggcgcg cccagggccg cggtcaggat
     4021 ctccacgagc gcctgccgca gcgcgtcggg cgtcctcgcc ccggcgcgcg cccgctcccg
     4081 gaggcgggcg atcaccgcat cggtcgtccg cacgccgaga tcggcctgga tgagcgtctc
     4141 ttctagcgcc tcatagaacc cttcgcggac gccccgctgc aggaggtcat cgacctgggc
     4201 ggtcagggac tgccgcgttc tggcgagccc ggtgcgaagg cgttcgagcc acccgggagg
     4261 gctagccaac cggctggggg gcgccgagcg tttccggatc caccagccgc atggagacca
     4321 tctgggagac gcctccctcc tccatggtga tccccaccag ggcgtcgctg gcctccatcg
     4381 tggccttgtt gtgggtgatg atgatcatct gcgttcgcgc cgacaactcc cggaagatct
     4441 cggccacctt gcgggtgttg gcctcgtcca gcgccgcttc gacctcgtcg aagacgcaga
     4501 aggggctggg gcggacccgc agcatggcga acaccaaggc cagcgccacc atcacgcgct
     4561 ccccgccgga gagggcgccg aggctgcgga gcttcttacc ggggggttgg gcgatgatct
     4621 cgatgccggg ctcggcgcca tcgaagccgg gaacgcgctc gagcccggcc cggccgccgc
     4681 cgaagatgcg gacaaacaga tcggcgaact ctttgttgac cgcctcgtag gtcctgtcga
     4741 actggccgcg gatctcctcc tcgaggtggg cgatgagggc ccgaagcgtc gtgagggtcc
     4801 cctggacgtc ctccatctgc gtgcgcagcc cccgcgcccg ctccgccacc tgccggtgct
     4861 cctcgatcgc caggagattg accggcccca acgccgcaat cagcccgcgg agcgcctcga
     4921 tccgcccgag cgcctcatcg cggttgacgc tctcggggac cgcgcccacc gcccggtcga
     4981 acgggacgcc aaactcctct tccatgcgcc ggcgcgcgct ccccagttcg gcctcgatct
     5041 gcgcctggcg gacctcggcc cggtgcatct cttcatcgag ggcggctgtc cggtcgaggg
     5101 cctcgcggtg gcgcgtctcg gatgcggcca gatccgcggc gagcccggcc cgctcggcct
     5161 cgagcgccgc ctgggccgcc tcgaggcggg ccccctcggc ctcgatcgct tcgcaccggg
     5221 agaggtgggc cgtgcgctcg gcgtgcaact gctcggcctc gagttggaac cgcccgagct
     5281 cgtcgtcgag ttcgtcgcac tgcgcggcca gctgggcgat tccgcgatcg acctcgctga
     5341 tgcgggcgcg caccgcctcg cggcgtccac tcacctcggt cagcgtcacc cggagttcgg
     5401 tgatccggga tgcggccgcg cggcgggcgc cggcgtggtc ccgcagcccg gctgcgatcc
     5461 ggccgatctg cgcctccacc tcggtcagcc gtgcgccgag gcccgcggct tcctcccgga
     5521 ggcggtccct gagcgtgtgc tgcgatgccg ccgcctgatc ggttgcggcc tgctccatgg
     5581 cgaggtgcgc gatctcctgg gcgaaccgct cgacctcggc gtcggccagg gtcagccggc
     5641 gccggcactc cgccaccgct tcggcctggc gggccaggag cgcctcggcc gccgcgatcc
     5701 ccccctcgag gtcgtggatg cgtgccccca gggccccgcg gcggcggccc agctcaccga
     5761 tggaccgctc gacgcgctcc agccccgcgg ggatctcggc catttcctcg gcccgcccga
     5821 ggatcccccc ctgttcgggc agccgccggc cggcgagcag gaatccctgg ggggcgagca
     5881 tctcgccggc cagggtgacg acccgtcccc tgtatccgtt cgcccgggct cggagggcgg
     5941 cgtccaggtc tgccacgatg aggacgtccg ccagcactgc acggatggcg tccggatggg
     6001 gaccggcgat cgtgatccga tcgacggccc ggcccaccgt cccggggtcg gctgccaccg
     6061 cagacggcaa caccggtaac tccggggctg ccaccgccga caccggcaca aacatcacgg
     6121 atccccgcgg ctcgccgccg aggagcgcca gcgcggcgcg cgcatcctcc acggaggaga
     6181 cgacgagcgc cgagagggcc ggccccagcg ccgtttcgaa cgccggctgg aggtcccgcg
     6241 gcacggtgag gaagtccgcg accgccccgc gcaggccggg cagcctggcc gggtgcgcgc
     6301 gccaggcgag caggatctcg cgggccccgg tctcataccc gcggtactga gcctgggcct
     6361 cctccaggaa ccgcaggcgg gaggcgatcc cctcccgctc gatctcgagc cggcgctcgt
     6421 cggcgaggag ggcgtcctgg ccggcgcggt gttcgtcgag ttccgcgcgg agctggacca
     6481 ggcggcgccg cgcctcctcg agcccggcca tcccctcggt ggattccttc ccgagctgag
     6541 actgctgggc gcgcgcggcg tccgcctgct cgcggaggta ggccccccgg cgatccagac
     6601 cggcgctccg gtcgcggtgc tccgcctcgc gggcctccgc agcggccacc tcggtatcgg
     6661 ccttcgcgcg cgcctcggtc agcgcccggg ccgcgacgcg gcaggcttcc agctcctcct
     6721 cgccccgcgc cgccgccgcg tccatcccgg ccaggtccac ctcgtgggcc tccagagcgg
     6781 cccgggcctc gtcctgccgg gtcgccagat cgcgctcggc ctcgtcgtgc gcgccccgct
     6841 cggtgcgcag cgcctcttgc tcggcgcggg cgcgggacac ctcccggtcg gtccgctgcc
     6901 ggtgggagtc gaccgcccgc aggcgctcgg ccaccagttc caccgcggcc tccgcggcgg
     6961 cgcggcgttg ggcgaggctc acccgatcct gctggaactg gtcccattcc cggccagcat
     7021 cggccgcccg gcggtccagc gccgcccgct gttccaccac cgcctgcacc gtgctcgtga
     7081 tctcccgtcg ccgggcggcg atctcctcga gttgatcacg cacgcgccgt tgggcgcggc
     7141 tcccccgccg gatctcctcg acctgaatgg acagctcgag ggtccgcagc tcccgggtgt
     7201 gggcctggta ttgttcagcc gcctgcgcct gggtggccag ctgggccgtc tgggcgtcga
     7261 gctccccgag gatgtcggtg acgcgcaaca gggtcgtctc ggtggccgcc atccggcgct
     7321 ccgcatcgtg ccggcggcgc ttgtacttgg cgagcccggc cgcttcctcg aggatcatcc
     7381 gccgttcatc cggcgtggcg tccagcatct gctcgacttc gccctgggtg atcagggcgt
     7441 acgagtgccc gcccaggccc gtgccgagga acatcgtctg gatgtcccgg aggcggcacg
     7501 ggagcccgtt gatgaagtac cggctctccg cattccgcat cccgcggcgg gtcacggtga
     7561 cctcggcaaa cgccagcggg gtcggcgggg cgtcgccgtt gtcggggtcc gggggcagca
     7621 caaggcttcc atcgtcgttg tccagggtca cggtcacctc ggccatcgcc agcggccgcc
     7681 gcttgtccgt gccggcaaag atgatgtctt cggtgcggac gccccggagc gaccggaggc
     7741 tgccctcccc cagcgcccac cggatcgcgt caaagacgtt gctcttcccg ctcccgttcg
     7801 gcccgacgat gccggtgatc tgggatgaaa actcgaacac cgtgcgttcc gcgaaggtct
     7861 tgaacccgct caactccagc cgcttcaggc gcacgtcatt cctccctgat gggttaggcc
     7921 ggtgcgtggg tcggcgcgtt gtgggcggcg atcacctcgt ggtacacgcc caccagttgc
     7981 tcgaccaccc gggcctggct gcgggaggcg gcccacgcct ttccccgctc ccccatcccc
     8041 cgccgcagcg cgggggtctc caagaggtcg gcgatcgccc ggcggagcgc cgctggatcg
     8101 ggggggacca ggcggccggt ctcgccatca cggacgatct ccgggatcgc ccccccgcgt
     8161 accgcgacga ccgcgcgccc cgccgccatc gcctccagca cggcgaggcc cagggtctcc
     8221 gtctgggacg gaaagacgaa gagatcgctg gcgtacagcg cctcgacgac gtacgcgtgg
     8281 tcctgctccc cggcaaacac gacgcgctcc gcaaggccga gccgctggac ctgggcctcc
     8341 agctcccggc gctcggg
//
LOCUS       DASQGH010000161         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54476, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000161 DASQGH010000000
VERSION     DASQGH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54476"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VEW91_03045"
     CDS             <1..789
                     /locus_tag="VEW91_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90596.1"
                     /translation="LFGSGGFFHISLLPVWKEALVAAPLLGLVGAGVYALGGDRAWLL
                     VCYWASPILAVAVVSHRVNVFYPRYFSFLTPPFALLLAAGIDLGAAALIRWARRGMES
                     RPVALAACTVIVLAMNAPVINGYVSVDYAEHDWRSAAELVTAAAGPTDYLLFVPGFAE
                     TPFEYYYKGSQERLRLTPVEIYETVRIKKAADPKINKVWAHHLAQDHPRVWIIATVPL
                     PGSAFLRLRALLGDDFGAGQAWDFHSVYVIGLQSLLYRAHAAAK"
     gene            854..1594
                     /locus_tag="VEW91_03050"
     CDS             854..1594
                     /locus_tag="VEW91_03050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYM90597.1"
                     /translation="MLPAYNEAENLPPLLDRVGAAWSGRLRFRVLVVDDGSRDATASV
                     AEAAGGRLPVEVIRHTHNRGLAAAIRTGIQEVCRRAKDDDAVVIMDADNSHPPELVPE
                     MVARLEAGDDIVIASRFVRGGREEGVPWRRRVLSRAAALVFRCVCPIPGVRDYTTGFR
                     AYRAAVLKELRHLYGDRLIEAAAFSVMTELLLKARPLRPRIAEVPLVLRYDLKGGASK
                     MRLGGTIRDYARLLGRELRPRRPGRRVV"
BASE COUNT          220 a          573 c          599 g          291 t
ORIGIN      
        1 ctgttcgggt cgggcgggtt ctttcacatc tcgctgctgc cggtctggaa ggaggccctg
       61 gtggcggccc cgctcctcgg gctggtggga gcaggagtct atgccctcgg gggcgatcgg
      121 gcctggctgc tcgtgtgtta ctgggcgagc cccatcctcg cggtcgcggt ggtgtcgcac
      181 cgggtcaacg tcttctaccc ccggtacttt tcatttctca cccctccgtt tgccctgctc
      241 cttgcggcgg ggatcgatct cggggcggcc gccctgatcc ggtgggcccg gcgggggatg
      301 gagagccgtc cggtcgcctt ggcggcgtgc acggtgatcg tgctggcgat gaacgcgccg
      361 gtcatcaacg ggtatgtgtc ggtcgactat gcggaacacg attggcgcag cgcagccgaa
      421 ctcgtcaccg ctgcggcggg ccccacggat tacctgctgt tcgtccccgg gtttgccgag
      481 acaccgttcg agtactacta taagggatcg caggaacggt tgcggctcac gccggttgag
      541 atctacgaga cggtgcggat caagaaagcg gcggatccca agatcaacaa ggtctgggcc
      601 catcacctcg cgcaggacca cccccgcgtg tggatcatcg cgacggtgcc ccttccggga
      661 agtgcgttcc ttcgactcag ggcgctcctc ggggacgact ttggcgccgg ccaggcctgg
      721 gactttcact cggtgtacgt catcgggctt cagagtttgc tctatcgcgc gcatgccgcg
      781 gcaaaatagt cgagcacccg cccccggctc ccgaagctcc gctgcgcccg ccgacgggcg
      841 tgcggtctgg ctggtgctcc cagcctacaa cgaggccgag aacctccccc cgctcctcga
      901 ccgcgtcggt gccgcgtggt cggggcggct tcggttccgc gttctggtgg tggacgacgg
      961 cagccgcgac gccacggcct cggtggcgga ggcggcgggg ggccgcctgc cggtcgaggt
     1021 catccggcac acgcataacc ggggtctggc cgcggcgatc cgcacgggga tccaggaggt
     1081 ctgccgccgg gccaaggacg acgacgcggt cgtgatcatg gacgccgata actcccatcc
     1141 cccagaactg gtgcccgaga tggtggcccg gctcgaggcc ggcgacgaca tcgtgatcgc
     1201 ctcgcggttc gtgcgcggcg ggagggagga gggggttccg tggcggcgcc gggtgctgag
     1261 ccgggcggcc gcactcgtct tccggtgcgt ctgcccgatc cccggggtgc gcgactacac
     1321 caccgggttt cgggcctacc gggccgccgt gctcaaggag ctccggcacc tctacggaga
     1381 ccgcctcatc gaggcggcgg cgttctcggt catgaccgag ctattactaa aggcccgccc
     1441 tctccggccg cgcatcgccg aggtccccct ggtcctgcgg tacgatttga agggaggcgc
     1501 aagcaagatg cgcctcggcg ggacgatccg ggactacgcg cggctcctcg gccgggaact
     1561 gcggccccgc cggcccgggc ggcgcgtcgt gtgatggccc gtcccgcgca ggcggtgatc
     1621 gatcctctcc ccgaggtcac gcaggcgtcc cacgcggcca gatctgggcg cctcctgggg
     1681 ctc
//
LOCUS       DASQGH010000162         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23380, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000162 DASQGH010000000
VERSION     DASQGH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23380"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VEW91_03055"
     CDS             <1..294
                     /locus_tag="VEW91_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HYM90598.1"
                     /translation="RARTLNSATIVAMYVMVAVLIALGRLTPFASIAAVALPRAGRAL
                     AVTARPKPLTPPAGYVGWPLWYHRVCLVHNRLFGWAYIAGLAIGAAVPSLRPG"
     gene            472..>1537
                     /locus_tag="VEW91_03060"
     CDS             472..>1537
                     /locus_tag="VEW91_03060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HYM90599.1"
                     /translation="MGPVDPNERVEVTVRMRSRSSTGLPSVEEMSARLPRDRQYLKRD
                     EFRDTHGADPQDLEKIRAFARRHGLTIVAEHVARRSVVLSGTAAAMSNAFGVELARYE
                     HAGKTFRGRSGPIYVPAELAAVVEGVFGLDDRPQAKPHFRLGAARGGAVVAAATATAV
                     APRAAAAMAYTPPQLAALYDFPSGTGRGQCIGIIELGGGYRPQDLSTYFAQLNTPTPQ
                     VVDVSVDGGQNAPTTPDSADGEVLLDIEVAGAIAPGARIAVYFAPNTDRGFLDAITTA
                     VHDSTNNPSAISISWGAAEANWTQQAMLSFDQAFQEAAALGVTVCCAAGDDGSSDGVQ
                     DGAPHVDFPAASTFVLGCGGT"
BASE COUNT          232 a          557 c          494 g          254 t
ORIGIN      
        1 cgggcaagaa cgctcaatag cgcgactatc gtggcgatgt acgtgatggt ggcggtcctc
       61 attgcgctcg ggcgcctcac tccgttcgcc agcatcgccg ccgttgcact gccgcgagcc
      121 gggcgcgccc tcgccgtgac ggcccgaccc aagccgctca cccccccggc cggctatgtt
      181 gggtggcctc tctggtacca tcgcgtctgc ctggtccaca atcgcctgtt cggctgggcg
      241 tacatcgccg gcctcgccat cggcgccgcc gtcccgagcc tgcgcccggg ctagcggcca
      301 agagtttctg gaagttctag gctccaatgc tgcaacactt ctgcgacttt atttgggcgc
      361 ttcacgcagg aattctcccc agcgcaacta accgatacag tgcctatgcc gtctcccaaa
      421 gaccgcgtcc tccttcccca cagcgagaag gccactcctc ccggaacccg ggtggggccg
      481 gttgacccca acgagcgcgt ggaggtcacc gttcgcatgc ggtcgcggtc gagcacgggg
      541 cttccatccg tcgaggagat gagtgcccgg ttgccccgcg atcgccagta cctcaagcgc
      601 gacgagttta gagacaccca cggtgcggac ccgcaggacc tggaaaagat ccgggccttc
      661 gcccgtcgac acggcctcac gatcgtcgcc gaacacgtcg cccggcgcag cgtggtcctg
      721 tcgggcacgg ccgctgccat gagcaacgct ttcggggtcg agctggcgcg ctacgagcat
      781 gccgggaaga cctttcgcgg gcgctccggc ccgatctacg tgccggccga gcttgcggcg
      841 gtcgttgagg gggtgttcgg tctcgatgac cgtccccagg cgaagcccca ctttcgcctt
      901 ggggcggccc gggggggagc cgtcgtcgcc gccgccaccg ccaccgcggt cgccccacga
      961 gcggcggcgg ccatggcgta taccccgccc caactggctg cgctctacga ctttcccagc
     1021 ggcaccggcc ggggtcagtg catcggcatt atcgagcttg ggggcggcta ccgcccccaa
     1081 gacctgagca cgtacttcgc tcagctcaat acaccgaccc cccaggtcgt ggacgtttcc
     1141 gtggacggcg gtcaaaatgc ccccaccact cccgacagcg ccgacggcga ggtgctgctc
     1201 gacatcgaag tggccggggc catcgctccg ggcgcgcgca tcgcggtgta ttttgccccc
     1261 aacacggacc gcggctttct cgatgccatc acgacggccg tgcatgacag caccaacaat
     1321 ccttcggcca tctcgatcag ctggggggcg gccgaggcga actggacgca gcaggccatg
     1381 ctctccttcg atcaggcctt ccaggaggcc gccgccctgg gcgtgaccgt gtgctgcgct
     1441 gcgggagacg acggctccag cgacggcgtg caggacggcg cgccgcacgt tgacttcccc
     1501 gccgcgagca cgttcgtgct ggggtgtggc ggcacga
//
LOCUS       DASQGH010000163         1740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14870, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000163 DASQGH010000000
VERSION     DASQGH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14870"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..868
                     /locus_tag="VEW91_03065"
     CDS             266..868
                     /locus_tag="VEW91_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HYM90600.1"
                     /translation="MFDALDELGVARAAALHDLRVFGTRARAAGPVETVQAEVRLDKS
                     YPVGDFSIGEQIRLCAHGSMLVIDLHGEPVSSWGALATRAAMRRGLAGCLILGGARDV
                     EEIDGLDFLLAAEHVTPRTGKGRIRFTGRGGPVQRGATRIEAGDWMVVDRTGGVVIPA
                     PAVAGAADRAVALEDADAQFVHLMEDGVGFDQAARRLGHF"
     gene            990..>1740
                     /locus_tag="VEW91_03070"
     CDS             990..>1740
                     /locus_tag="VEW91_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HYM90601.1"
                     /translation="MTTAHDAFYAQDVPENGRKRSLLALSYAEVAERLKEPGQDVVLV
                     PLGSTEKHGAHIPLGTDSYITMAAVVQAAELADCLYAPLVPFGYSPHHMGRLHEGAGT
                     ITLQAETYRRIMHDVGLSLIFHGFNRVIFVSHHGSNTKPIDEILRHLRYRTGAFFAFY
                     KTPTEREVNVLEGVLENPPEETPGWHSSELETSCLMAVHQGLVSMKRAVSDRAHAPRW
                     MGSKFSKTDGTGTVKFQGSENIWVPMEHHEYS"
BASE COUNT          286 a          563 c          638 g          253 t
ORIGIN      
        1 ccagacctca tctgggagtg cagctacccc accctcgagg agcgggagcg catccgcgcc
       61 tttcaggaag gcgatccggc gttccacgcc atccgcacca gacagggcgc gcagcttgcg
      121 cactgggcgc gcgagcgcta ccggatcgtg gaatggcggt aggctggaaa aggagcggtg
      181 acggccatgg ggggggcaca cgaagccacc gggcaaccca gcgggcgatg tcgcgagccg
      241 gctctgccgg tgctcgtcga gcagcatctt tgacgcgctg gacgagctcg gggtggcacg
      301 cgcggccgcc ctgcacgacc tgcgggtgtt cgggacgcgg gcgcgggccg cggggccggt
      361 cgagacggtg caggccgagg tgcgcctcga caagagctac ccggtcggcg acttttcgat
      421 cggcgagcag atccgcctgt gcgcgcacgg ctcgatgctc gtcatcgacc tgcacgggga
      481 gccggtatcg agttgggggg cgctcgcgac gcgggcagcg atgcggcgag ggctcgccgg
      541 ctgcctgatc ctcggcgggg cccgggacgt cgaagagatt gacgggctgg acttcctgct
      601 cgccgcagag cacgtgacgc cgaggaccgg caagggccgc atccggttca ccgggcgcgg
      661 cggacccgtg cagcgtggcg cgacccggat cgaggccggc gattggatgg tggtggatcg
      721 caccggcggg gtggtgatcc ccgcgcccgc ggtcgccggc gccgccgatc gggccgtggc
      781 tctcgaggac gccgacgcgc agtttgttca tctgatggaa gacggcgtgg ggttcgatca
      841 ggcggcccgc cggctgggtc acttctaagc cgacgcgggg cagaggcccg cgctccccca
      901 cgcccgcgcc gcgcgcgcat tgacaagcga gagccaccgg cctacgatcg cctcggaaaa
      961 ggtctttccg tcgcgggagg ggaggggccg tgacaacggc gcatgatgcg ttctacgcac
     1021 aggacgtgcc ggagaacggg cgcaagcgca gcctgctggc gttatcctat gccgaggtgg
     1081 ccgagcgcct gaaggagccc ggccaggacg tggtgctggt ccccctcggg agcacggaga
     1141 aacacggggc gcacatcccg ctgggcaccg acagttacat taccatggcg gccgtggtcc
     1201 aggccgccga gctggcggac tgtctgtacg cgcccctcgt accgttcggc tattctccgc
     1261 accacatggg acggctgcac gagggcgcgg ggacgatcac gctgcaagcg gagacctacc
     1321 gccggatcat gcacgatgtg ggcctcagcc tgatcttcca tggattcaac cgggtcatct
     1381 tcgtcagcca ccacgggtcc aacaccaagc cgatcgacga gatccttcgg catctccgtt
     1441 accggacggg ggcgttcttc gccttctaca agacccccac cgagcgcgaa gtgaatgtcc
     1501 tcgaaggggt gctcgagaac ccgccggagg agaccccggg ctggcattcc agcgagctgg
     1561 aaacgtcctg tctgatggcg gtccaccagg ggctcgtgtc gatgaaacgg gcggtctcgg
     1621 accgcgcgca cgccccccgg tggatgggct cgaagttctc gaagaccgat gggacgggca
     1681 cggtgaagtt tcagggctcg gagaacatct gggtgccgat ggagcaccac gaatactcgg
//
LOCUS       DASQGH010000164         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34679, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000164 DASQGH010000000
VERSION     DASQGH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34679"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1117)
                     /locus_tag="VEW91_03075"
                     /pseudo
     CDS             complement(1..1117)
                     /locus_tag="VEW91_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1257..1859
                     /locus_tag="VEW91_03080"
     CDS             1257..1859
                     /locus_tag="VEW91_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90602.1"
                     /translation="MKIARLLAGPCVVLLVAALLRPAGAQPGWQPVADPKGLFSLRLP
                     SDWKIADSELSAVVFRGLRSSAAGRYLLSTLAAHGPSSDADGMGILAVAAMGLPRRVS
                     PAEFGEQFKAEAPSWWAVTQEGSAHIAGRDAYYLYFVMTQKGITLYMVVAYFTVGQTG
                     FLVMGGTLNDPVAIRKHFATISKILETFRPSASLGALRVR"
     gene            complement(1860..2717)
                     /locus_tag="VEW91_03085"
     CDS             complement(1860..2717)
                     /locus_tag="VEW91_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90603.1"
                     /translation="MIGQHPARPSGELVRALRRNRLAVGGACLLGLVLWCAAFAPLIA
                     PQDPNLLRPEIRLQSPSAHHWFGTDGFGRDMYSRVIYGARVSLLVGALVALLTTVAGV
                     FFGMLAGYFRAWDGTIMRVMDALLAIPPVLLAIAIVASLGPRISNVVVSLTVAYTPQL
                     ARVLRSQVLVLREATFTEAARSEGASEWLIAARHVLPNALSPVIVQGSFTFAYAVLTE
                     AALSFLGVGAPPYIPSWGNELNDGRPFMVQAPWITVFPGLAIITCVLGLNLFGDGLRD
                     VLDPRLRRD"
     gene            complement(2782..3729)
                     /locus_tag="VEW91_03090"
     CDS             complement(2782..3729)
                     /locus_tag="VEW91_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90604.1"
                     /translation="MFTFIVRRLAALVPVLLVVAVFVFSLIHITPGDPAAYMLGPSAS
                     PAEVEHLRTVLGLNLPLYQQFVIWLGRAVRGDLGQSIFMGIPVSVSIAQRLEPTFLLT
                     LLAVVFMVVIGIPAGTLAAARRNTPADQVVMAVSVLGLSVPSFWLGLNLILLVGVKLR
                     WLPVAGYVPMAQGDVWASVRTVLMPAFSLGFINAALIARMTRSSMLEVLGLDFVRTAR
                     AKGLPRAAVIWRHALGNAILPVLTAIGNSFTVLLGGLVVTEQVFAIPGVGQLVINAVL
                     RRDYPVIQGAVLYIVALYIAINIGLDVLYAAADPRIKYG"
     gene            complement(3811..>4304)
                     /locus_tag="VEW91_03095"
     CDS             complement(3811..>4304)
                     /locus_tag="VEW91_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYM90605.1"
                     /translation="HRPFYIRRARPSGQESRGAGAKGNHAMHTMSGAHPLAAAAREWL
                     AALQRCVRAVDYPGARHLFAPEVQGFGTHAAIVGGLEALEREQWTQIWPGIREFTFRL
                     GEVRCAGSEELLGVIVPWDSLGVNPDGSSFPRPGRATLILARREGRWVAIHSHFSLAP
                     LGR"
BASE COUNT          676 a         1445 c         1551 g          632 t
ORIGIN      
        1 cctggaggag cgctttggcg cccgcgacgt cctggtggtt gaacacgcgc gcgcccgcgg
       61 tgttgtcggc gaaggctccg taggcgatcg tcggcgagtc gatgctccag aactcctgcg
      121 gtccgaacgc ggcctgcatg atcggcttcg tgtcgagcgc cttcatgaag gcctggcgcg
      181 ccttcacgct ggtgaagagg cccttctgtt tgttgaagac gaacgtcgcc caggcgtagg
      241 gcttcacgat ctcggggacc aggttgggag tgcccttcac ctgcgggtac tggtcctcgg
      301 gaagctggag cgcgaagtcg tactggccgg tctgcatccc ggcgaggcgg gtgttggctt
      361 cggggaccgg gatgaacagg atctcatcga cgtacgcggt gcgcccgccg gcgtagcccg
      421 aggcgggggc ctccaccggg cggtagccgt tgaagcgcac gacccgcacg tgggtgtcgg
      481 gtctccactc ctggaacttg tacggtcacg tcccgaccgg cgcggtgatg ggtttgtcgc
      541 cgtactgatc gatgatgcgc ttgggcatga tcgccggcat gttgttgggg ttggcgagcg
      601 ccgcgatgag cgagccgacc ggctggttga ggtcgatcgc gaccgtgtgg gcgtctctcg
      661 cggtgacggc cttgaccttg ggcgcgatcg cctgcccaag cgcggagagc cgcatccacc
      721 gcccgagcga cgcggccgcg tcggtcgccg tgaggggcag gccgttgtgg aagaccacgt
      781 cgctccggag cgggaccacg agcgtcaggt tgttgttgga ccagctgtac ccgctggcca
      841 ataggggccg gggctcccag ttgccgtcga gggcaaagag ggtctcgtag atctcctgcg
      901 tcacctgctc ggcgatcacc gcggtcgtct ggtggatgtc caccgtcggc ggatcgccga
      961 tcgtggcgat cttcaggatg ccgcccgttc gcggggcctg cgccaccgcg ggctgctcgg
     1021 tgcacaccgc ggagaggccc cccgccgcgc ccgcggcgag cagtccgccc gcgcgacgca
     1081 gaagttctct ccgggaaagg ttgggcacgg tgcgcatcgt tccaccccca gcctggcgat
     1141 agacccgagg ccctgattcg ctgcgcctgg agcgactcct ggtctccgtg ccgcccgacg
     1201 ccggcggttg ggcgtcccgt ctgagcgcgg gccatccggg gtatgtgttg gatattatga
     1261 aaatcgcccg cctgctcgcc ggtccatgcg ttgtgctcct cgttgccgcg ctcctccggc
     1321 ctgccggagc ccagccgggt tggcagccgg tagcggaccc caaaggactg ttcagcctcc
     1381 ggctcccctc cgactggaag atcgcggact ccgagttgag cgccgtggtg tttcgtggcc
     1441 tgcggagcag cgccgcgggc cgatatctgc tctccacgct cgcggcgcac ggtccgtcga
     1501 gcgacgccga cgggatgggc atcctcgcgg tggccgccat gggcctcccc cggcgcgtat
     1561 ctcccgccga gttcggcgag cagttcaagg ccgaggcccc gtcctggtgg gcggtgacgc
     1621 aggaaggctc ggcgcacatc gccggccgcg acgcgtacta tctgtacttt gtcatgaccc
     1681 agaagggtat cacgctgtac atggtcgtcg cgtacttcac cgtcgggcag acggggttcc
     1741 tcgtgatggg ggggaccttg aacgacccgg tggccatccg gaagcacttt gccacgatct
     1801 cgaagatcct cgagacgttt cggccgagcg ccagcctcgg ggcgctacgg gtccgctgat
     1861 cagtcgcgcc ggagccgagg gtcgagcacg tcgcgcaatc cgtcgccgaa caggttcagg
     1921 cccagcacac aggtgatgat cgcgagcccg ggaaagacgg tgatccaggg cgcctggacc
     1981 atgaacgggc gcccgtcgtt cagctcattg ccccacgagg ggatgtaggg cggcgcgccg
     2041 acgccgagga agctgagcgc cgcctcggtc agcaccgcgt acgcgaaggt gaacgacccc
     2101 tggacgatga cgggggagag cgcattgggg agcacgtggc gagcggcgat cagccactcg
     2161 ctggcacctt ccgaccgtgc cgcctccgta aacgtcgcct cccgcaagac cagcacctgc
     2221 gaccgcagca cccgggccag ctggggcgtg tacgcgacgg tgagcgagac gaccacgttg
     2281 ctgatccgcg gccccaggga cgccacgatg gcaatcgcga gcagcacggg cgggatcgcc
     2341 aggagcgcgt ccatgacacg catgatggtg ccgtcccacg cgcggaagta gccggccagc
     2401 atcccgaaga agacccccgc gaccgtcgtg agcagcgcga cgagcgcgcc gacgagaagc
     2461 gagacccgcg cgccgtagat tacgcggctg tacatgtccc ggccgaagcc gtcggtcccg
     2521 aaccagtggt gcgcgcttgg ggactgcagc cggatctccg ggcgcagcag gttgggatcc
     2581 tgcggggcga tgagcggcgc gaaggcggcg caccacagca cgagcccgag caggcacgcg
     2641 ccccctaccg ccagccggtt gcgccggagc gcccgtacga gctcgccgct cgggcgggcg
     2701 gggtgttgcc cgatcacggc gcggaccgcg ttcgtacttc ttcctcctct ccccgtgccc
     2761 caaatgcggg ggccgccgcg ctcagccgta cttgatgcgg gggtcggcgg cggcgtagag
     2821 cacgtccagg ccgatgttga tcgcgatgta cagcgccacg atgtacagca cggccccctg
     2881 gatgaccggg tagtcgcgcc ggagcacggc gttgatgacc agctggccga cgcccgggat
     2941 ggcaaacacc tgctcggtga cgacgagccc tccgagcagc acggtgaagc tgttgccgat
     3001 cgccgtcagg accggcagga tggcgttccc cagcgcgtgg cgccagatca cggcggcccg
     3061 gggcaacccc ttggcgcgcg ccgtgcggac gaagtcgagc ccgaggacct ccaacatgct
     3121 ggaccgggtc atccgcgcga tgagcgcggc gttgatgaag ccgagcgaga acgccggcat
     3181 gagcaccgtg cgcacggagg cccacacgtc cccctgcgcc atcggcacgt agcccgccac
     3241 cggcagccac cgcagtttca ccccgaccag gaggatgagg ttgagtccca gccagaagct
     3301 cgggaccgag aggccgagta cggacacggc catgaccacc tgatcggccg gggtgtttcg
     3361 gcgtgccgcc gcgagggtcc cggcgggaat gccgatcacg accatgaaga cgacggcgag
     3421 gagcgtgagc aggaacgtcg gctcgagccg ctgcgcgatc gagacggaca ccgggatccc
     3481 catgaagatg gactgaccca ggtccccccg caccgcgcgg ccgagccaga tgacgaactg
     3541 ctggtacagc gggaggttga ggccgagcac cgtccgcagg tgctcgacct ccgcggggga
     3601 ggccgacggc cccagcatat aggcggccgg atcgccggga gtgatgtgga tcagcgagaa
     3661 cacgaacacg gccaccacga gcaggaccgg cacgagcgcc gcgagccggc ggacgatgaa
     3721 ggtgaacagg ggttttccct acctcgagac tcggatgatg ttcccgtgct ttcttggcgg
     3781 cggggcgacc tcctgcccgg cagcccgcgg ctagcgcccc aacggcgcca gcgagaagtg
     3841 cgagtggatc gccacccacc ggccctcccg ccgggcgagg atgagcgtgg cccgcccggg
     3901 ccggggaaac gacgagccgt ccggattgac gccgagcgag tcccacggga cgatcacgcc
     3961 cagcagctcc tcgctgcccg cgcagcggac ctccccgagg cggaaggtga actcgcggat
     4021 gccaggccag atctgcgtcc attgctcgcg ctcgagcgcc tcgaggccgc cgacgatggc
     4081 ggcgtgggtc ccgaagccct ggacctccgg cgcgaagagg tggcgggcgc ccggatagtc
     4141 gactgcccgc acacagcgct ggagggcagc tagccactcg cgggcggccg cggcgagcgg
     4201 gtgggcgccg gacatggtgt gcatcgcgtg gtttcccttt gctcccgcgc ctctggactc
     4261 ctggccggag ggtcgggcgc ggcggatgta gaacggacga tggc
//
LOCUS       DASQGH010000165         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_715, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000165 DASQGH010000000
VERSION     DASQGH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VEW91_03100"
     CDS             complement(<1..691)
                     /locus_tag="VEW91_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM90606.1"
                     /translation="MTVLSDRPAPRPLIPWTIEWRRVPAPVRYAALILALVAAWQVYL
                     RLAHVSPLLVASPADVGQALAAGIRDRRIPDATASTLLLLVSGIVIGGVTGFVFASVA
                     VFSALGRDVLAVLIAVMNPLPSIAILPLAMIWFGLTPKAIVLVVALATIWPVAINADA
                     GFRAISPTTHMVARNLGLRGPRMVLGVLLPAALPYILAGVRAAWAFGWRTVVAAELVF
                     GVAGSTGGLGWY"
     gene            complement(688..1524)
                     /locus_tag="VEW91_03105"
     CDS             complement(688..1524)
                     /locus_tag="VEW91_03105"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90607.1"
                     /translation="MPLDASPSGSAAAPLVEAQDVRIMYDAFCAIDGASFTVRRGEKF
                     VIIGPSGCGKTTLLKAVAGFIRPAGGVILVNGAPVGEPGPDRAVVFQDFEQLFPWRTV
                     LSNITYALAVAAGVRGEAGRDRARRMLDLVGIAHAAGRFPHQLSGDMKQRAAIARALA
                     LEPQILLMDEPFGALDEITRSRLGVELNEIWRRTGVTIILVTHSIPEAVFLGHRVMVM
                     GDRAGRVREIVDTEAAEAWGDPAFEALTDHLRGVLIAPPPAASEAGAAAGRSPTASRV
                     TP"
     gene            complement(1524..2492)
                     /locus_tag="VEW91_03110"
     CDS             complement(1524..2492)
                     /locus_tag="VEW91_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90608.1"
                     /translation="MLRRQTLLIVLTLAAWPGGTGAAAAPDAPRTVTIAYQPGIGYAT
                     LLIIKAQHTLEKQFPGTTFDWKLLANGAVIRDGLIAGTIQFGSGGVGPFLIGWDRGAG
                     LHLIASMNEMNLWLVTLDPHIQSLRDIKPGMKIGMPGPDSIQAIVLRKAAQQQLGNAH
                     ALDNAIVAIEHPVGVQALLHGQLAAHLASPPFQFDEVAAGGHVILQSYDLFGRSTFNS
                     VFTTDKVYHAYPKLGDAMYRDLAGASAFVSAHPEEAAAILSQDAGGKPAAGQFLAWMT
                     HQGVAYTTTPHGFLQYATFMHAIGLLTKVPGSMCEIELPPLHCLGN"
BASE COUNT          417 a          943 c          979 g          373 t
ORIGIN      
        1 tgtaccaccc caggccgccg gtcgaccccg cgacgccgaa cacgagctcg gcggcgacga
       61 ccgtccgcca cccgaacgcc caggcggccc ggacgccggc caggatgtac ggcagcgcgg
      121 ccggcagcag cacccctagg accatgcggg ggccccgcag accgaggttg cgggcgacca
      181 tgtgcgtcgt cgggctgatc gcgcggaacc ccgcgtccgc gttgatcgct accggccaga
      241 tcgtcgccag cgcgacgacc aggacgatcg ccttcggcgt gaggccgaac cagatcatcg
      301 ccagcggtag gatcgcgatc gagggcaggg ggttcatcac ggcgatcagc acggcgagca
      361 cgtcgcggcc gagcgcggag aacacggcga ccgaggcgaa cacgaagccg gtaacgccgc
      421 cgatcacgat gccgctgacg agcaggagga gcgtgctcgc ggtcgcgtcg gggatccgac
      481 ggtcacggat cccagccgca agcgcctggc ccacgtcggc cggcgaggcg acgagcagcg
      541 gggacacgtg ggccagccgg aggtacacct gccacgcggc gacgagcgcg aggatgagcg
      601 ccgcgtaccg gaccggcgcc ggcacgcgcc gccactcgat cgtccatgga atcagcggac
      661 gcggggccgg ccggtcggac agcaccgtca cggcgtcacc cggctcgccg tgggggaccg
      721 tcccgccgcg gcgccggcct cggaggcggc gggcggaggc gcgatcagca cgccgcgcag
      781 gtggtccgtg agcgcctcga acgccgggtc cccccacgcc tccgcggcct cggtgtccac
      841 gatttcccgg acgcgtccgg cccggtcgcc catcaccatc acgcggtgtc ccaagaaaac
      901 cgcctccggg atggagtggg tgaccaggat gatcgtcacc ccggtccgcc gccagatctc
      961 gttcagctcc accccgaggc ggctccgggt gatctcgtcg agggcgccga acggctcgtc
     1021 catcaggagg atctgcggct ccagcgcgag cgcgcgggcg atcgccgcgc gctgcttcat
     1081 gtcgccggag agctggtggg ggaaccggcc ggcggcgtgg gcgatgccga ccagatcgag
     1141 catgcgccgg gcccggtcgc ggcccgcctc gccccgcacg ccggccgcga cggcgagagc
     1201 gtaggtgatg ttgctgagga cggtgcgcca cgggaacagc tgctcgaagt cctggaagac
     1261 cacggcccgg tcgggtcccg gctcccccac cggtgcgccg ttcaccaaga tcactccgcc
     1321 ggcgggccgg atgaacccgg cgacggcctt gagcagggtc gtcttgccgc acccggacgg
     1381 gccgatgatc acgaacttct cgccccggcg gaccgtgaag ctcgcgccgt cgatggcgca
     1441 gaacgcatcg tacatgatcc gcacgtcctg cgcctccacg agcggcgccg cggcgctgcc
     1501 ggacggggat gcgtccagcg gcactaattg cccaggcagt ggaggggcgg gagctcgatc
     1561 tcgcacatcg accccggcac cttggtcagt aggccgatcg cgtgcatgaa cgtcgcgtac
     1621 tgcaagaacc cgtggggcgt cgtcgtgtag gcgacgccct ggtgggtcat ccacgccagg
     1681 aactggccgg cggccggttt gcccccggcg tcctgcgaga ggatcgccgc ggcctcctcg
     1741 ggatgcgcgc tcacgaacgc ggacgctccg gcgaggtcgc ggtacatcgc gtcgccgagc
     1801 ttcgggtacg cgtggtacac cttgtcggtc gtgaacacgc tgttgaacgt gctccggccg
     1861 aacaggtcgt agctctgaag gatgacgtgg ccgccggccg cgacctcgtc gaactggaac
     1921 ggcggcgagg cgaggtgcgc ggccagctgg ccgtgaagga gcgcctgcac gccgaccggg
     1981 tgctcgatcg cgacgatggc gttatcgagc gcgtgggcgt tcccgagctg ctgctgggcg
     2041 gccttccgca ggacgatcgc ctggatcgag tcggggccgg gcatgccgat cttcatgccg
     2101 ggtttgatgt ccctgaggga ctggatgtgc ggatccagcg tcacgagcca tagattcatc
     2161 tcgttcatcg aggcaatcag gtggagcccg gcgccccggt cccacccgat cagaaacggg
     2221 ccgacgccgc ccgagccgaa ctggatcgtg cccgcgatca ggccgtcacg gatcacggcc
     2281 ccgttggcga gcagcttcca gtcgaacgtc gtgccgggga actgcttctc cagcgtgtgc
     2341 tgagccttga tgatcagcag ggtcgcgtag ccgatgcccg gctggtaggc gatcgtgacg
     2401 gtccgcggcg catcgggggc ggcggcggct ccggttcccc ccggccaggc ggccagcgtg
     2461 agcacgatca gcagtgtctg ccggcgcagc atcggttcta cctcctcccg cacccacgga
     2521 gcagtgacgg cgccttccgc ggggcagcgc tgacatcctt ctccgtctcc gccgcggaac
     2581 accgaggcgc cgggcgggta gggtcgcggc tggcggagga aaaacggatc tggacacgat
     2641 ttgtcggctg atcaccaggc gctgaatctc ggacgtccct tcatcaggat ttggcgtttt
     2701 tcctgcggga tg
//
LOCUS       DASQGH010000166         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20567, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000166 DASQGH010000000
VERSION     DASQGH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20567"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VEW91_03115"
     CDS             complement(<1..332)
                     /locus_tag="VEW91_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYM90609.1"
                     /translation="MTFPHDRPRRLRRTDRVRAMVRETTLEPCHLIAPLFVKEGLPRP
                     AEIETMPGQQHHTVSSLVEACAGLAELGVPAVMLFGIPARKDAQGSAAWDPEGIIQQA
                     LRAVRAELG"
     gene            complement(355..1122)
                     /locus_tag="VEW91_03120"
     CDS             complement(355..1122)
                     /locus_tag="VEW91_03120"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYM90610.1"
                     /translation="MITRSRAQAGNLRALLEAQGAEVIEFPSIRVAPLDDYTAVDRAI
                     AQLGEYRWLVFTSQNGVAAFVDRLRVCGRDPAELGRLRLAAIGPATSRALETFGPRPA
                     VAPRRFVAEALVEAMAGEDLRGVRVLLPRALDARAVLPDGLRALGAVVDVVPVYRIET
                     ERGQSPHALSRLLAGAVDCVTFTSSSTVRNFVEVLGPEASRILGSVTVACIGPVTAAT
                     AREHGLSVGVVADVYTIPGLVDALRDRLGRGVPAFDR"
     gene            complement(1152..2114)
                     /gene="hemC"
                     /locus_tag="VEW91_03125"
     CDS             complement(1152..2114)
                     /gene="hemC"
                     /locus_tag="VEW91_03125"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYM90611.1"
                     /translation="MTVKRGVLRVGTRGSRLSLRQTQIVLDLVRDRVPGVQFEVAIIQ
                     TAGDRAPAVPLERLEGIGFFARELEAALLDGRCDLAVHSAKDLPTEVHGDLCLAAFPP
                     RGDPRDVLIDRAGRRLATLPPGARVATSSVRRSAQIRHRRPDLDTVSIRGNIDTRLAK
                     LDRGVCDALCLAGVGLVRMGWENRIAEWLDPEVMLPAPGQGAIAVEMRRRDAGLQDLV
                     RRINDPATQGAVEAERAFVGRLGSGCRAPTAALAAVEGEIITLEGMVALPDGSIVRRH
                     RASAPATGAERLGISVADDLLAHAGFVLDAVRAAPLPSPSGGGL"
     gene            complement(2154..2759)
                     /locus_tag="VEW91_03130"
     CDS             complement(2154..2759)
                     /locus_tag="VEW91_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYM90612.1"
                     /translation="MSRFEEGRGDRPRWLTLGEAAHFLGVDATTLRGWADAGKVRAFR
                     TPGGHRRFDPAHLETLVQASAPSTPSAPVPTIAPQEWLASRPWYAGIDEPARARVRGH
                     CAELMRIVTAYFGGAPAHPRHLAAARQVGAALGQEVAGWGLTPAQSTEVFLHFKLHVT
                     EALTTPQEGGGMGRLRSMRDADAFLGEVLQAMMEAYGTSRG"
     gene            complement(2806..3012)
                     /locus_tag="VEW91_03135"
     CDS             complement(2806..3012)
                     /locus_tag="VEW91_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90613.1"
                     /translation="MYLRCLVTREWAWYEPSSFLTAPEGGRARTVGAGPSRPRPAARR
                     RAPAARRPRRAAAPKTRRKGGKKR"
     gene            complement(3096..>3218)
                     /locus_tag="VEW91_03140"
     CDS             complement(3096..>3218)
                     /locus_tag="VEW91_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HYM90614.1"
                     /translation="RLSQVMEYFEIFLPRMVMCRHAAKLLGCEFKLQINGTKLL"
BASE COUNT          465 a         1212 c         1107 g          434 t
ORIGIN      
        1 ccgagctcag cccgcaccgc tctgagggcc tgctggatga tcccctcggg atcccatgcc
       61 gcgctgccct gggcgtcctt gcgggccgga atcccaaaca gcatgacggc cggcacgccg
      121 agttccgcca gcccggcgca ggcctcgacg aggctcgaca ccgtatggtg ttgctgcccg
      181 ggcatcgtct cgatctccgc cgggcgcggc aggccttcct tcacaaagag gggcgcgatc
      241 aggtggcacg gttccagggt ggtctcgcgg accatcgcgc gcacccggtc ggtgcgccgg
      301 agccgtcgtg gccggtcatg ggggaaggtc atcgcctgat acctccgtct gcgtctatcg
      361 gtcgaatgcg ggaaccccgc gccccaaccg atcccgaagc gcgtccacca agccggggat
      421 cgtgtagaca tcggccacga ccccgacgct gagcccgtgc tctcgggccg tcgcggcggt
      481 gaccgggccg atgcaggcga cggtcacgct gccgaggatt cgggatgcct ccggccccag
      541 cacctcgacg aagttccgca ccgttgagga actggtgaac gtcacgcaat cgaccgcccc
      601 cgcaaggagc cggctcaacg cgtgggggga ttgtccgcgc tccgtctcga tgcgatagac
      661 ggggacgacg tccaccaccg cgccgagggc gcgcagcccg tctggcagga cggcgcgggc
      721 gtcgagcgcc cgcggcagca gcacgcgcac gccccgcagg tcttccccgg ccatcgcctc
      781 cacgagcgcc tcggccacga acctccgagg agccacggcc gggcggggcc cgaaggtttc
      841 gagcgcgcga gacgtggccg ggccgatcgc ggcaaggcgc agccggccga gctcggcggg
      901 atcgcgaccg cagacccgca ggcggtccac gaacgcggcc acgccgttct ggctggtaaa
      961 gaccagccac cggtattccc cgagctgcgc gatcgcccgg tcaacggccg tgtagtcgtc
     1021 cagcggagcc accctgatcg acgggaactc gatcacctcg gctccctgcg cctcgagcag
     1081 cgcgcggagg ttgcccgcct gcgcgcggga ccgggtgatc acgatcctcc gcccactgag
     1141 ccgtgaggcg gtcataggcc gccaccggag ggagaaggga ggggtgcggc ccgaacggcg
     1201 tcgagcacga acccggcgtg cgcgaggaga tcgtctgcca cggagatgcc gagccgttcc
     1261 gccccggtgg ccggcgcgct tgcccggtgg cggcgcacga tgctgccgtc gggcaacgcc
     1321 accatcccct cgagggtgat gatctcgccc tcgaccgcgg ccaaggcggc ggtgggggcg
     1381 cggcacccgc tccccaggcg ccccacgaac gcccgctcgg cctcgaccgc cccctgtgtg
     1441 gccgggtcgt tgatgcggcg cacgaggtct tggagccctg cgtcccgccg gcgcatctca
     1501 acggcgatcg ccccctggcc gggggccggc agcatgacct cggggtcgag ccattcagcg
     1561 atccggttct cccagcccat ccgcaccagc cccaccccgg cgaggcagag cgcatcgcag
     1621 acccctcggt cgagcttggc gaggcgcgta tcgatgttcc cgcggatgga cacggtgtcc
     1681 aggtcgggac ggcggtggcg gatctgggcg ctccgccgga cgctgctcgt ggccacgcgc
     1741 gcgcccggcg gcagggtcgc gagccggcgg ccggcgcggt cgatcagcac gtccctcggg
     1801 tctccccgcg gcgggaacgc cgcgaggcac agatcgccgt ggacctcggt tggaagatcc
     1861 ttggcgctgt gcacggccag atcgcatcgc ccgtcgagca gggcggcctc cagttcacgg
     1921 gcgaagaacc cgatgccttc gagccgctcg aggggtaccg cgggcgcgcg gtcgcccgcg
     1981 gtctggatga tcgccacttc gaactgcact cccggaacgc ggtcgcggac gaggtcgagg
     2041 acgatctggg tctggcgcag gctcagccgg ctcccccgcg tccccacccg cagcacaccc
     2101 cgcttcaccg tcaccccccg ctcctcccgg aaccgatggc cgtcccccga ggtctacccg
     2161 cgggaggtcc cgtaggcctc catcatcgcc tggaggacct cgcccagaaa ggcgtcggcg
     2221 tcccgcatcg atcggagtcg ccccatgccc ccgccttcct ggggagtagt gagcgcctcc
     2281 gtcacgtgca gtttgaagtg cagaaacact tcggtggatt gcgcgggcgt cagcccccac
     2341 cccgccacct cctggcccag ggctgccccg acctgccggg cggcggcgag atggcggggg
     2401 tgggcagggg cacccccgaa gtacgcggtc acgatccgca tcaactcggc gcagtgcccc
     2461 cggactcggg cccgggcggg ctcgtcgatc ccggcatacc acggacggga tgccagccac
     2521 tcctgcgggg cgatcgtcgg cacaggggcg gacggtgtcg acggggcgct cgcctggacc
     2581 agcgtctcca ggtgcgcggg atcgaagcgc cggtgccccc ccggcgtccg gaacgcgcga
     2641 accttcccgg catcggccca tccccggagc gtggtcgcgt caaccccgag gaagtgggcc
     2701 gcctcgccga gggtcagcca gcgcggacga tcccctcggc cctcctcgaa tctggacaca
     2761 gcctaggaga ttctaaggaa ttctatggaa tcctttcgag ctgacctacc gcttcttgcc
     2821 ccccttccgg cgggttttcg gagccgcggc ccggcgcggc cttcgcgcgg cgggcgcccg
     2881 ccgccgggcc gccggccggg ggcggctcgg ccccgcgccg acggtgcgcg cccgcccccc
     2941 ctcgggggcg gtgagaaagc tcgacggctc gtaccacgcc cactcgcggg ttacgaggca
     3001 gcggaggtac atcgtcccct ccggccgctg cgctgcggcg tgggtcccac cgcaggactt
     3061 gcaccggtac tgccaagaag ccatcggcgt cccccctaca gcagtttcgt cccgttgatc
     3121 tgcaacttga actcacaccc cagcagcttc gccgcatggc gacacatcac catccgcggc
     3181 aggaagatct caaagtactc catcacctgg gacaaccg
//
LOCUS       DASQGH010000167         4836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23411, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000167 DASQGH010000000
VERSION     DASQGH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23411"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VEW91_03145"
     CDS             complement(<1..321)
                     /locus_tag="VEW91_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYM90615.1"
                     /translation="MRYALTLPAMLERAEQVFPQKEIVSRTASGIFRYTYREYGQRVR
                     RLASALLALGVRKGDRVATLAWNQHRHLEAYFAVPSIGAVLHTCNLRLPPAHLAHIMN
                     HPEDR"
     gene            complement(441..1730)
                     /gene="serS"
                     /locus_tag="VEW91_03150"
     CDS             complement(441..1730)
                     /gene="serS"
                     /locus_tag="VEW91_03150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYM90616.1"
                     /translation="MVDLKLIRDQPDAMREALRKKRADPNLIDRVLEADRRRRELVQQ
                     VEALRAAQNRASQEIPRLAGAAREERIAEMKRIAAQAKAQEPALQEADAALGALLLQI
                     PNPPHASVPEGGPDDSVALRVVGTPPRFDFAPRDHVDLGAALDLLDVERSAKVSGSRF
                     YFLRRDGALLQLALIRYAVDRLCAEGFIPTVPPVLIKRETLIGMMGGNTLDEQMVYRI
                     EGEDLALAGTSEMALGSMLAGEVLSEAGLPIRLCGISTCFRREAGTHGKDTRGMFRVH
                     QFDKVEMFSYCHPDRSWQEHDYLVDLEERLWKDLGLPYRVVNIAAGDLGDPAARKYDL
                     ETWMPGRGGYGETQSCSNCTDFQARRLNIRFRPSGGGGLEFVHTLNGTAVACTRAIIA
                     ILENFQQADGSVRIPPPLVPYMGGRTVLRPPSPVSAR"
     gene            complement(1841..2989)
                     /locus_tag="VEW91_03155"
     CDS             complement(1841..2989)
                     /locus_tag="VEW91_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYM90617.1"
                     /translation="MRETNLLIPGPTPLPPRVLHAMGQQMLNHRGPAFGRIMTEMLTG
                     LREVFQTRNDIIPLVCSGTGGLEASVVNLLSPGDRVLSVNNGNFCERYAAIAERYGAR
                     VDRVLAAWGQPVPLDAIAERIRADARGEYRAVLVTQSETSTGVHNDVRAIRGVLGEHP
                     ALLMVDAVSSLGAIPLETDAWGVDVAITGSQKALMSPPGMAFVSVSDRAWAATERSTI
                     PRFYLDLGRGRMQVHQSHPSTPFTTAVTVAYAVHEALRLIHEEGLERVFERHRRMARM
                     VRAGVRGMGLSLLPDDAHAVDTVTTVRMPPGLDAQQVVAHARERYGIVFGRGIGRLEH
                     TIVRIGHLGYTQPEMLTSGLEGLGRTLGDLGHPVDTGAGLEAAAAQRP"
     gene            complement(3338..4267)
                     /locus_tag="VEW91_03160"
     CDS             complement(3338..4267)
                     /locus_tag="VEW91_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYM90618.1"
                     /translation="MAINLHQLMLFHTVARSGSFSRAAAELLISQPSVSIQVRELERQ
                     LGADLFEQVGKSVRLTEAGRVLDDYATRILGLIDEARVAVDEIKGLRRGRLLLGATPT
                     PGTYVLPALLGRFKEQYPGIEIVLSIKDTRRIQEMVLQHELDLGVVGGKVSFPDLEAS
                     VFLTDELVLVAAPSHRFAALPAVRVADLAGEPFILRERGSGNREVVDEALHQAGVHVT
                     PVFELEGAEMVKQAVAASLGISILWRCAVALDVAAGRLRIVPLEGLEMKRAIWLVHRR
                     DHRLPRAAQAFLEMLRPAATAPLPAAASETPAR"
     gene            4399..>4836
                     /gene="selD"
                     /locus_tag="VEW91_03165"
     CDS             4399..>4836
                     /gene="selD"
                     /locus_tag="VEW91_03165"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:4450..4452,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYM90619.1"
                     /translation="MNDRDRIRLTTLVACAGUASKLGPADLAQVLRHLPPITDPAVLV
                     ATNTADDAGVYRIAPDLALVQTVDVFTPIVDEPYWFGAIAAANALSDVYAMGGTPRVA
                     LNIAGFPRSKLPLDVLGEILRGAADKCTEAGVVVIGGHTLEDPE"
BASE COUNT          710 a         1703 c         1701 g          722 t
ORIGIN      
        1 ccggtcctcc gggtggttca tgatgtgggc caggtgggcg ggggggagcc ggaggttgca
       61 cgtgtgcagg accgcgccga tcgacgggac ggcaaagtag gcctcgaggt ggcggtgctg
      121 attccacgcc agcgtggcga cccggtcgcc cttgcgcacg cccagcgcca ggagcgccga
      181 cgccagcctg cgcacccgct gcccatactc ccggtaggtg taccggaaga tccccgatgc
      241 ggttctggac acgatctcct tctgggggaa gacctgctcg gcccgctcca gcatcgccgg
      301 cagcgttagc gcgtaccgca tcgtcgccac agtaggtcac ctcccgctga ccgaggcccg
      361 agttcgtggg tcgttcgtgg ccccccggcc ggagtccttg ccgccttatt agaagtcctg
      421 gggaccctgt accagccccg tcagcgcgcc gacacgggcg acggtggacg cagcaccgtc
      481 cgtcccccca tgtacgggac gagcggcggg gggatgcgga cgctgccgtc cgcctgctga
      541 aaattctcga ggattgcgat gatcgcgcgc gtgcaagcga cggcggtccc gttgagcgtg
      601 tggacgaact cgagcccccc gcccccgctc ggccggaagc ggatgttgag gcggcgggcc
      661 tggaagtccg tgcagttgct gcacgactgg gtctccccat accccccccg gccgggcatc
      721 caggtctcga gatcgtactt gcgggccgcg ggatcgccga ggtctccggc cgcgatgttc
      781 acgacacggt aggggagccc gaggtccttc cacagccgct cctctaggtc cacgaggtag
      841 tcgtgctcct gccacgaccg atccggatgg cagtacgaga acatctccac cttgtcgaac
      901 tggtggaccc ggaacatccc gcgcgtgtcc ttcccgtgcg ttcccgcttc gcgccggaag
      961 caggtggaga tcccgcagag ccggatcggc agccccgctt cgctcaggac ctcgcccgcc
     1021 agcatggagc ccagcgccat ctccgaggtt ccggcgagcg ccaggtcctc gccttcgatc
     1081 cggtacacca tctgttcgtc cagcgtgttg ccgcccatca tgccgatcag ggtttcccgc
     1141 ttgatcagga ccggtgggac ggtggggatg aacccctcgg cgcacaatcg gtcgaccgcg
     1201 taccggatga gcgccagctg caagagcgcg ccatcccggc ggaggaagta gaaccgcgac
     1261 cctgagactt tggcgctgcg ctccacgtcg aggaggtcga gggcggcgcc gaggtccaca
     1321 tgatcccgcg gcgcgaagtc aaaccggggc ggcgtgccca ccacgcgcag ggcgacgctg
     1381 tcgtcggggc cgccttcggg caccgacgca tgcggggggt tgggaatctg gaggagcagc
     1441 gccccgagcg cagcgtcggc ctcctgcagg gcgggctcct gagccttggc ctgcgccgcg
     1501 atgcgcttca tctcggcgat ccgctcctcg cgggccgcgc cggcgaggcg ggggatctcc
     1561 tgcgacgccc ggttttgggc ggcccggagc gcttcgacct gctggacgag ctcccgccgg
     1621 cggcggtcgg cctcgagcac gcggtcgatg aggttgggat cggcgcgctt cttgcgcaac
     1681 gcctctcgca tcgcgtctgg ctggtcacgg atcagcttga ggtcgaccat cgccacccac
     1741 ccctccccgt gcccgacggc gtcgcaaagg cacaccgtag ccggccaagc cttcctcggc
     1801 cagcccgtcg gccaccagca cgcgcaccgg ggccgccatg ctacggccgc tgcgccgccg
     1861 cggcctcgag cccggcgccg gtatccactg ggtgcccgag gtccccgagc gtccggccca
     1921 agccctcgag gccggaggtc agcatctcgg gctgcgtata ccccaggtga ccgatcctga
     1981 cgatcgtgtg ctccagccgc ccgatgcccc ggccgaatac gatgccgtag cgctcgcgcg
     2041 cgtgggcgac cacctgctgc gcgtcgaggc ccgggggcat ccgcacggtc gtgacggtgt
     2101 cgacggcgtg ggcgtcatcg ggcaggaggg agagccccat gccgcggacg cctgcgcgga
     2161 ccatccgggc catccggcgg tgccgctcga acacgcgctc caacccttcc tcgtggatca
     2221 gcctgagggc ctcgtgcacg gcgtaggcca cggtcacggc cgtggtgaac ggcgtgctcg
     2281 ggtgcgactg gtggacctgc atccgcccgc ggccgaggtc caggtagaac cgcgggatcg
     2341 tcgagcgctc cgtggccgcc catgcgcggt cgctcacgct gacgaaggcc atccccggcg
     2401 ggctcatcag ggccttctgg gatcccgtga tggcgacgtc caccccccac gcgtccgtct
     2461 cgagagggat cgcgcccaga ctgctgaccg catccaccat cagaagggcg ggatgctcgc
     2521 ccaggacgcc gcggatggcg cggacgtcgt tgtgcacccc ggtgctggtc tcgctctggg
     2581 tgaccaggac cgcccggtac tcgccacggg cgtccgcgcg aatccgctcc gcgatcgcgt
     2641 caagcggcac cggctgcccc cacgcggcga gcacccggtc gacgcgcgcg ccgtaccgct
     2701 cggcgatcgc ggcgtaccgc tcacagaagt tgccgttgtt gaccgagagc acccggtccc
     2761 cgggcgagag gaggttgact accgacgcct cgagcccgcc ggtgcccgag cacaccagcg
     2821 ggatgatgtc gttgcgggtc tgaaacacct cccgcagccc ggtcagcatc tccgtcatga
     2881 tgcggccgaa ggccggcccg cgatggttga gcatctgctg gcccatcgcg tggaggaccc
     2941 ggggcgggag gggcgtcggg ccggggatca gcaggttggt ctcgcgcatc ggatgcctcc
     3001 tccagacgat tagacgatcg cccgcgtgat gaaacgacaa cgctcccgcc caaggaggcg
     3061 agagcgctcg cggtgccact cctcatccag gcgcggatca ccccgcagcc cggcactcgg
     3121 gtgcggtatc gggcagaccc ggcagcctcc ggcacgtgcc gcgcggctgc ggctcgggag
     3181 cggacccggc cggtcgcctc cgccgggttc caccaccacc ggctctctag agggggcgcg
     3241 caggccgagg aatctccgtc atcgcgttcc tggtgcgctg gtcgtgctga tgatagcatc
     3301 ggcgccgaac gccgtcaacg cggggcgcgt cgggtggtca gcgggcgggg gtttcggagg
     3361 cggccgcggg gagcggcgcc gtcgccgccg gcctgagcat ttcgaggaag gcctgggccg
     3421 cccgaggcag gcggtgatcc cgccggtgga cgagccagat cgcgcgcttc atctccaggc
     3481 cctcgagcgg cacgatgcgg aggcggccgg cggccacatc cagtgcgacc gcgcaccgcc
     3541 agaggatcga gatccccagg ctggcggcca cggcctgctt caccatctcg gcgccctcca
     3601 gttcgaacac cggggtgacg tgcaccccgg cctggtggag cgcctcatcg acgacctcgc
     3661 ggttccccga gccgcgctcg cggaggatga acggctcgcc ggcgagatcg gcgacccgca
     3721 ccgcggggag cgcggcgaac cggtgcgacg gcgccgccac gagcaccagc tcatcggtga
     3781 gaaacacgct cgcctcgagg tcggggaagc tcaccttgcc gccgacgacg cccaggtcca
     3841 gttcgtgctg cagcaccatc tcctgaatcc gccgcgtgtc cttgatggac aacacgatct
     3901 cgatgccggg gtactgctcc ttgaaccggc ccaggagcgc ggggagcacg tacgtcccag
     3961 gcgtgggggt ggcgccgagc aggagccggc cgcgccggag tcctttgatc tcgtctaccg
     4021 cgacccgggc ctcgtcgatc aacccgagga tgcgggtggc ataatcgtcc agcacgcggc
     4081 cggcctcggt cagacgcaca gacttgccca cctgctcgaa cagatcggcg cccaactgcc
     4141 gttcgagctc gcggacctgg atgctgacgg agggctggct gatcaggagc tccgccgccg
     4201 cccgggagaa gctgccggat cgagcgacgg tatggaagag catgagctgg tgcaggttga
     4261 ttgccatcgg acgtcaccat attactatat agcatgcgtg ttcgtcattg gcgggcgcgg
     4321 gcccgccgtg cctgcgctgg cgggagacgt cgctgcgggg gatgttacaa tgagggcacg
     4381 ctacgcacgg ggtgagggat gaacgatcga gaccggatca ggctgacgac cctggtcgcc
     4441 tgcgccggct gagcctcaaa actgggtccg gcggacctgg cgcaggtcct gcgccatctt
     4501 ccccccatca ccgatcctgc cgtcctggtg gcgaccaaca ccgcggacga tgcgggggtg
     4561 tatcgtattg ccccggacct ggccctggtc cagaccgtgg acgtgttcac gcccatcgtg
     4621 gacgagccgt actggttcgg cgcgatcgcg gcggcgaatg cgctgagcga cgtgtacgcc
     4681 atggggggca cgccgcgggt cgcgctcaac attgcggggt ttccccgctc gaagctgccg
     4741 ctggatgtgc tcggcgagat cctccggggc gccgcggaca agtgcaccga ggccggggtc
     4801 gtggtcatcg ggggccacac gctcgaggat cccgag
//
LOCUS       DASQGH010000168         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31940, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000168 DASQGH010000000
VERSION     DASQGH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31940"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1062
                     /locus_tag="VEW91_03170"
     CDS             109..1062
                     /locus_tag="VEW91_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90620.1"
                     /translation="MRRVRGVALAAALVLSTPVWGAAPAGGWIQGRVIDRTRPQHPVA
                     HQRVRLTMIERGASSEQEAVTDASGVVRFTRLPVGGIRVFVLSTDYRSVRYTSDRIVL
                     ADTAPARPVDLAVYEPSPDRSKVRAPVAFAVVDIARRTIRVSVIQRFENPTDRAMVGS
                     PETPWVFPLPPGAGSVSYLAGWQDPQTGRGRITDVLTLLPGTAQVAYSYQLEARESRL
                     VLPWSLPYGAGDVEVLVADAGVGVAAEGLRAQGMVAGPQGGRYQRWSGGPVAPGGEVV
                     LRLRGVPVGGKAWPGVVAAGLGLILGVGLAAALRRSKRPAV"
     gene            1269..>1828
                     /locus_tag="VEW91_03175"
     CDS             1269..>1828
                     /locus_tag="VEW91_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYM90621.1"
                     /translation="MALVAALALTGCQPAVDISRAKVDRSLPPAQEVLIPLVIDENWH
                     KIVPDAIRGKQVFEQHCAVCHGMNGDGKGPMAYTLVAPDKDVLTAFLGLFGITLHRPS
                     LSDRPFSFANRDIENVVTPAILFETVTLGRPHLAMPAFGPEASFGANKAETLSNADRW
                     DANVYEMMFRTTPEELSTAKALYNAQ"
BASE COUNT          243 a          619 c          684 g          282 t
ORIGIN      
        1 cgccgcggcg gtcgcggcat gggtgctcgc cccgctccgg ccgccggttg ctccacgtgc
       61 atcggcgccc gccgcacccg cggaggatcg cccgccgggc tgacggccgt gcgcagagtg
      121 cggggtgttg ccctggcggc cgcgctcgtc ctctcgacgc ccgtctgggg cgccgcgccg
      181 gccggtggct ggatccaggg acgggtcatc gatcgtaccc ggccgcagca cccggtcgcg
      241 catcaacggg tccggctcac gatgatcgag cggggggcgt cctccgagca ggaggcggtg
      301 accgatgcct cgggcgtggt ccggttcacc cggctgccgg tcggggggat ccgcgtgttc
      361 gtcttgagca ccgactaccg gagcgtgcgg tatacaagcg accggatcgt gctcgccgac
      421 accgctcccg cccgcccggt ggacctggcg gtctacgagc cgtccccgga tcggtcgaag
      481 gtgcgcgccc ctgtggcgtt cgccgtcgtg gacatcgcca gacgtaccat tcgggtcagc
      541 gtgatccaac ggtttgaaaa ccccacggac cgcgcgatgg tcggctcccc cgagactccg
      601 tgggtgttcc cgctgcctcc gggagccggg tcggtgagct atctggcagg gtggcaggac
      661 ccccagaccg gccgcggccg gatcacggac gtgttgacgc tcctgccggg gaccgcgcag
      721 gtggcctact cgtaccagct cgaagcgcgg gagtcccgct tggtgctgcc gtggtcgctc
      781 ccgtacggtg cgggggacgt ggaggtgctc gttgccgacg ccggggtcgg cgtggccgcg
      841 gaggggctcc gcgcccaggg catggtcgcc ggaccacagg gaggacgcta ccagcgctgg
      901 agcgggggcc cggtggcccc gggcggtgag gtcgtgctgc ggcttcgcgg ggtgccggtg
      961 gggggtaagg cctggccggg ggtggtggcg gcgggcctgg gcctgatcct cggcgtgggc
     1021 ctcgcagcgg cgctgcggcg gtcgaagcgg ccggcggtct gagcgcgcgc ccgccgcccc
     1081 gtggctggag gagacccgga cgtccccgtg gaaaccccat ggcccgtacc cccacgcggc
     1141 gtttccgacg cgatgccagt gctgcgtcca cggatcgtgg tcccgatagg ctgcctgcgg
     1201 ttttgaagca cggcggaggt cagacccgaa tgcggttgag gagccaaagc gcccgacccg
     1261 gagcccttgt ggcgctagtt gctgcgctgg cgctcaccgg ctgccagcct gcggtcgata
     1321 tctcgcgggc caaggtggac cggagcctgc ccccggccca ggaagtgttg atcccgctcg
     1381 tgatcgacga gaactggcac aagatcgtgc cggacgcgat cagggggaag caggtcttcg
     1441 agcagcactg cgcggtctgc cacggcatga acggcgatgg gaaggggccg atggcgtaca
     1501 cgctggtcgc gccggacaag gacgtcctca ccgcgttcct cggcttgttc ggcatcacac
     1561 tccatcgccc ctcgctttcg gacaggccgt tcagcttcgc caaccgcgat atcgagaacg
     1621 tggtcacgcc ggcgatcttg ttcgagactg tcacgctggg gcggccccac ctggcgatgc
     1681 cggcgttcgg gcccgaggcg tccttcggtg ccaacaaggc ggagacgctc tcgaacgccg
     1741 atcggtggga cgcgaacgta tacgagatga tgtttcgcac gacccccgag gaactgagca
     1801 ccgccaaggc cctgtacaat gcgcagtg
//
LOCUS       DASQGH010000169         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43147, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000169 DASQGH010000000
VERSION     DASQGH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43147"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VEW91_03180"
     CDS             <1..944
                     /locus_tag="VEW91_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HYM90622.1"
                     /translation="SLPLLGLSVLAVGLVLLPHIGHVAGGARRWLSIGGAINFQPAVL
                     AKLGMILYLSNFLANRGGRVREFGPGLLPPLLVFLILAIAILKQPDLGSVLILAMITG
                     IMLFVGGARIAHLVGIGCAAVPAALAVILSEGYRSSRLLAFLDPWKDPRGAGFHIIQS
                     LLALGSGGVAGLGLGHSTQKFFYLPERHTDFIFAIIGEELGLVGTFAVILLFVLLAIW
                     GYRIASRLPDRYGILLTTGLTTMLVGQAALNIGVVTGALPVTGVPLPFISFGGSSLVS
                     CYLAIGILLNLSQYAPLAPGTRAAATPAPSRRAAPGR"
     gene            944..>1603
                     /locus_tag="VEW91_03185"
     CDS             944..>1603
                     /locus_tag="VEW91_03185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYM90623.1"
                     /translation="MNILLAGGGTGGHVYPALAVAEALRRHDPAVRVLFMGSRAGMEA
                     SLVPAAGVPFVGLAVRAPRSWTPVRLVLSAASVGVSLVRAFAVVAHFRPDAMIATGGI
                     AALPAVIAGAVQGTPVIVLEGNTIPGRTNRILARYSRLVAVSSDEAAVRLPAGRTVVT
                     GLPIRREVYTATREEGLGAFGLDPGRRTVLIIGGSQGAARLNTAAGEAVDHLAARGDL
                     QV"
BASE COUNT          182 a          567 c          583 g          271 t
ORIGIN      
        1 ggtcgctgcc cctgctgggc ctctccgtcc tcgccgtggg cctcgtcctc ctcccccaca
       61 tcggccacgt cgccggcggc gcgcggaggt ggctgtcgat cggaggcgcc atcaacttcc
      121 aacccgccgt actggcgaag ctggggatga tcctctacct gagcaacttc ctcgccaacc
      181 gcggcgggcg ggtgcgcgag ttcggtcccg gtctgctccc gccgctgctc gtgttcctga
      241 tcctggcgat cgcgatcctg aagcagcccg atctcgggtc ggtcctgatc ctcgcgatga
      301 tcaccgggat catgctcttc gtcggcggcg cgcggatcgc gcacctcgtc gggatcgggt
      361 gtgccgccgt ccccgcggcg ctggcggtga tcctgagcga aggctaccgc agcagccggc
      421 tgctggcgtt tctcgatccc tggaaggacc cgcggggggc cggcttccac atcatccagt
      481 cgctgctcgc gctggggtcg gggggggtcg ccgggctcgg gctcggccac agcacgcaaa
      541 agttcttcta cctgcccgag cgccacaccg acttcatctt cgccatcatc ggcgaagagt
      601 tggggctggt ggggaccttc gcggtgatcc tcctgttcgt gctgctggcg atttgggggt
      661 accgcatcgc cagccgcctc ccggaccggt acgggatcct gctgaccacg gggttgacca
      721 ccatgctggt cggccaggcc gcgctcaaca tcggcgtggt caccggcgcc ctgccggtca
      781 ccggcgtgcc gttgccgttc atcagctttg gaggatcctc gctcgtgtcc tgctacctcg
      841 cgatcggaat cctgctgaac ctgtcccagt acgcccccct cgcgcctgga acccgtgctg
      901 cggccacgcc ggcgccctcg cggcgcgccg ccccgggacg gtgatgaaca tcctgctcgc
      961 cgggggcggc accggagggc acgtctaccc ggccctggcg gtcgcggagg ccctccgccg
     1021 gcacgatccg gcggtccggg tgctgttcat gggcagccgg gccggcatgg aagcgtccct
     1081 ggttcccgcc gccggcgtcc ccttcgtggg gctggccgtc cgcgccccgc ggagttggac
     1141 gcccgtgcgc ctggtcctgt cggcggcgag cgtcggggtg agcctggtgc gggcctttgc
     1201 ggtggtagcg catttcaggc cggatgcgat gatcgcgaca ggggggatcg cggcgctgcc
     1261 ggcggtgatc gcgggagcgg tccagggcac ccccgtcatc gtgctggagg gcaatacgat
     1321 cccggggcgg acgaatcgga tcctggccag gtacagccgc ctggtcgccg tctcctcgga
     1381 cgaggccgcc gtgcggctgc ccgccgggcg gaccgtggtc acggggttgc cgatccgccg
     1441 ggaggtctac accgcgacgc gcgaggaggg tctcggggcg ttcgggctgg atcccggccg
     1501 caggacggtg ctgatcatcg gcgggagcca gggggcggca cgcctcaaca cggccgcggg
     1561 ggaggcggtg gatcacctcg cggcccgggg agacctccag gtg
//
LOCUS       DASQGH010000170         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12114, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000170 DASQGH010000000
VERSION     DASQGH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12114"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VEW91_03190"
     CDS             <1..1261
                     /locus_tag="VEW91_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYM90624.1"
                     /translation="ALAAYTVDGVRPAAVARPGSGEETARLLTAAGEHGAGVLPRGAG
                     VHQHLGGVPARADLVIDTGRLTGITDYTPADYVVAVRAGTRLRDLQAELGKHDQWLPL
                     DPPGAGAATIGGLIAANRNGPRRLLYGSIRDMLIGIRVALPTGEVIKAGGKVVKNVAG
                     YDLTKMFIGSLGAAGVIIEATFKISPVPGEGQTILVTVPDRAAAHELTTAIMGSYLLP
                     AALEAASPHALHHITQAAGMGAPSPHAWGVLVLAEGLAESRVRHIDEVRAVAGARGHV
                     EVLDGAAHTAVWQSVAEFPSPDIHPHGVVCRAGGPIARWAELSRGAEEARGQGARVEV
                     LAHAGVGVLYAACAAEDGPSVAASLGAAASAAGGYAVVEAAPPALKPHLPLWGAPPGG
                     LDLMRRLRAQFDPRGIMVPGRLGWGLS"
     gene            1246..>2266
                     /locus_tag="VEW91_03195"
     CDS             1246..>2266
                     /locus_tag="VEW91_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM90625.1"
                     /translation="MGALVTRRAIVTVAAMLGMFLAAIDQTAVGTAMPSITRALGGLA
                     LYSSVFAAYQIAFVASTPIFGRLADLYGRKGIYLIGILGFIGASVLCGLAQRMDQLVL
                     FRLLQGIGGGAVLPIALTIIGDLYPVEQRLKIQGMFSGVWGLAAIVGPLLGGLLADHG
                     NWRWIFFLNVPAGLAATAIITVAFRDPSTPRPGRVDYAGIGLLSSAAVALLLLLLWGG
                     QRFPWLSPAAAGLAALCALLGFLFVQVEARIPQPFLPFSLFEDRVVTVSSLGGFLLGA
                     SMFGAVVYIPLFAQGVIGMSATAAGIVLAPFMLCWTFASIVGGRWALRWGYRPVMAGG
                     MVLLVG"
BASE COUNT          253 a          803 c          855 g          355 t
ORIGIN      
        1 ggccctggcc gcgtacaccg tggacggcgt gcgccccgcc gccgtcgccc gcccgggatc
       61 cggcgaggaa accgcccgcc ttctcaccgc cgccggcgag cacggcgcag gggtcctgcc
      121 ccgcggcgcg ggggtccacc agcacctcgg cggggtgccg gcgcgcgcgg acctggtgat
      181 cgacaccggg cggcttacgg ggatcacgga ctacacgccg gccgattacg tggtggcggt
      241 gcgcgccggg acgcggctcc gcgaccttca agcggagctc ggcaagcacg accagtggct
      301 tccgctcgat cccccggggg ccggggccgc cacgatcggt gggctgatcg cggccaaccg
      361 gaatgggccg cgccggctgc tctacggctc gatccgcgac atgctgatcg ggatccgggt
      421 ggcgcttccc accggcgagg tcatcaaagc gggcggcaag gtggtcaaga acgtggccgg
      481 ttacgacctc acgaagatgt tcatcgggtc gctgggggcc gcgggcgtga tcatcgaggc
      541 gacgttcaag atctcgccgg ttcccggcga gggccagacg atcctcgtca ccgtcccgga
      601 ccgggccgcc gcccacgagc tcaccacggc gatcatgggc tcctacctgc tgcccgcggc
      661 gctcgaggcc gccagccccc acgccctcca ccacatcaca caggccgcag gcatgggcgc
      721 gcccagcccg cacgcgtggg gggtgctggt gctcgcggaa gggctggccg agagccgcgt
      781 gcgccacatc gatgaggtgc gcgcagtcgc gggagcccgg ggacacgtcg aggtgctgga
      841 cggggcggcg cataccgccg tgtggcagtc ggtcgccgag ttcccgtccc ccgacatcca
      901 tccacacggg gtggtgtgcc gcgccggcgg cccgatcgcg cgctgggccg agctgagccg
      961 cggcgcggag gaggcccggg ggcagggcgc ccgggtcgag gtgctggcgc acgcgggggt
     1021 cggggtgctc tatgccgcct gcgcggcgga ggacgggccg tccgtggccg cctccctcgg
     1081 cgccgctgca tcggcagccg gtggctacgc cgtggtcgag gcggcgccgc ccgcgctgaa
     1141 gccgcacctg ccgctgtggg gcgccccccc gggggggctc gacctgatgc gccgcctgcg
     1201 cgcgcagttc gatccccggg ggatcatggt cccggggcgg ctggggtggg ggctctcgtg
     1261 acccgccggg cgatcgtgac cgtggccgcg atgctcggca tgttcctggc cgcgatcgac
     1321 cagaccgcgg tcgggacggc gatgcccagc atcacgcggg ccctcggggg gctggcgctc
     1381 tactcctcgg tgtttgccgc ctaccagatc gccttcgtgg cgtcgacgcc gatcttcggg
     1441 cggctcgccg acctctacgg acggaagggc atctacctga tcggcatcct gggatttatc
     1501 ggcgcctcgg tcctgtgcgg gctggcgcag aggatggatc agctggtcct ctttcgcctc
     1561 ctgcagggga tcggcggcgg cgcggtgctg ccgatcgcct taacgatcat cggcgacctg
     1621 tatccggtgg agcagcgcct caagatccag gggatgttca gcggcgtgtg gggcctcgcg
     1681 gcgatcgtcg gccccctgct cggcgggctg ctcgccgatc acggcaactg gcggtggatc
     1741 ttcttcttga acgtgcccgc ggggctggcc gcgacggcga tcatcacggt cgcgttccgc
     1801 gatccgtcga ctccccgtcc ggggcgggtg gattacgccg ggatcggcct cctctcgagc
     1861 gcggcggtgg ccctcctgct cctcctcctc tggggcgggc agaggttccc ctggctgtcg
     1921 cccgccgccg ccggactggc ggcgctctgc gccctgctcg gcttcctctt cgtccaggtg
     1981 gaggcgcgga tcccgcagcc gttcctgccc ttcagcctct tcgaggaccg cgtggtgacg
     2041 gtctcatcgc tcggcggctt cctgctcggg gccagcatgt tcggcgccgt ggtctacatc
     2101 ccgctcttcg cccagggcgt catcgggatg agcgcgaccg cggccgggat cgtgctggcc
     2161 cccttcatgc tgtgctggac gtttgcgagc atcgtcgggg gacgttgggc gctgcggtgg
     2221 ggataccgac cggtcatggc gggcgggatg gtcctgctgg tgggcg
//
LOCUS       DASQGH010000171         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6434, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000171 DASQGH010000000
VERSION     DASQGH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6434"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..835
                     /locus_tag="VEW91_03200"
     CDS             350..835
                     /locus_tag="VEW91_03200"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM90626.1"
                     /translation="MDAGVRGLAAYLDETWDALLKGVEKVDDPLLHWTPGPEFNSVAI
                     LLRHLAGSERWWIGEAIGGVPSHRVRDKEFLHDRPRREEVLRAVEEARRVTRQVLSPL
                     TVQDLQAETAPSVTQGTPPRRPTKLWALLHYIEHLGYHRGQALLLLKLARNAPREASP
                     R"
     gene            849..1307
                     /locus_tag="VEW91_03205"
     CDS             849..1307
                     /locus_tag="VEW91_03205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HYM90627.1"
                     /translation="MRWDLCRKTHARLRKIVGALGFTHVDPSRIFCVRGYGSTSEAWA
                     RIWGLPTLWQQVLEIRPAYVVEIIEPEFSRLPRAEQDRVLIHELLHIPRTFSGAIRPH
                     RTPAFRINRKTVERFYQRYLASSRHPRARPSRLRLLRARSSRRQEKRSPR"
     gene            complement(1381..2097)
                     /locus_tag="VEW91_03210"
     CDS             complement(1381..2097)
                     /locus_tag="VEW91_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HYM90628.1"
                     /translation="MTELLYMRDSYLRESDATVAAVEDNAVALDGTVFYPGGGGQPAD
                     TGWLRWPGGESRVGEMRRDGETVWHVLDGPTPPAGAHVRGVLDWDRRYAIMRHHSALH
                     VLVGAVYRLFSARVTGGAIYPDRARMDFSLEDLSKERVAAIEQETNRVIAEDRRILVR
                     FVSREEFERSDLIQLARDLVPRDVERIRVVEIEGYDAQADGGTHVARTREIGRLRIVK
                     TDNKGKANRRLEIQLDHDTA"
     gene            complement(2123..>2682)
                     /locus_tag="VEW91_03215"
     CDS             complement(2123..>2682)
                     /locus_tag="VEW91_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HYM90629.1"
                     /translation="GDRMTNRVLPEPPQFPAGPFVADEAHDDRRRELWIDEIQRVPAR
                     LREVVSGLSGEHLDTRYRNWTIRQIVHHLADSHLNSYVRFKLALTEQRPTIKPYDESR
                     WSLLADAQRADVEPSLRLLEGLHARWAHLLRSLAPDAFERAFFHPETQEIVPLGRALA
                     YYVWHARHHTAQIEWVKTHKLPRIR"
BASE COUNT          403 a          932 c          894 g          453 t
ORIGIN      
        1 catcaggccg tacgatctcg gtaccatgcc tttctgcagt tgctcgggct tcttgccgac
       61 gagcgccatc cacgttgtgc cgaatacggc gggcgagtac cagatggcgc cgatcaccat
      121 gttgatgatg gctgcgacca ggaccgccac atagttgatg tgcacctgcg gcatcgcgct
      181 cacctccgag gctacgcgtt ccatcgcggg gtgcgggttc cctgccgggc ggcggtacgc
      241 gggttgttga tcgtcccatg cgcggcaggg acgggcgttg tcgcgcggcg aatgtaggga
      301 agagaccgca gggcgcgggt agccgcctgt gcggggaggg aggcgtgcaa tggatgcggg
      361 ggtgcgggga ctcgcggcgt atctcgatga gacgtgggac gcgctcctga aaggggtcga
      421 gaaggttgac gatccgctgc tgcactggac cccgggcccg gagttcaact ccgtggcgat
      481 cctcctccgc catctggcgg gaagcgagcg gtggtggatc ggcgaggcga tcggcggagt
      541 cccgagccac cgggtccggg acaaagagtt cctccacgat cggccccggc gggaggaggt
      601 gctccgcgcg gtcgaggagg cccggcgcgt gacgcgccag gtgctcagtc ccttgacggt
      661 gcaggacctg caggccgaaa cggccccatc ggtgacccag ggcacgcccc cgcgccggcc
      721 gaccaagctg tgggcgctgc ttcactacat cgagcacctc gggtaccatc gcgggcaggc
      781 tctgctcctg ctcaaactcg cgcggaacgc cccacgcgag gccagtcccc gctgatcgag
      841 gcctggcgat gcgctgggac ctgtgccgca agacccacgc ccgtctccgg aagatcgtcg
      901 gcgcgctggg cttcacccac gtcgatccgt cgcggatctt ctgcgttcgg gggtacgggt
      961 cgacgtcgga ggcgtgggcg cgaatctggg gcctgccgac cctgtggcag caggtgctgg
     1021 agattcgccc cgcctacgtc gtcgagatca tcgagccgga gttcagccgc ctcccgcgcg
     1081 ccgagcagga tcgcgtcctg attcacgagt tgctgcacat cccgcggacc tttagcggcg
     1141 cgatccggcc gcaccggacg ccggcatttc ggatcaaccg aaagacggtc gagcgcttct
     1201 accagcggta cctggcgagc agccgccacc cccgtgcgcg gccgtccagg cttcggctcc
     1261 tccgggcccg ctcctcccgc aggcaggaga aacggtcacc ccgctaggcc ggcgagagac
     1321 gccgggcgat cacagttcca cggcgtcata gttcatctcg tccccctgta cgaaggcgcc
     1381 tcaggcggtg tcgtggtcga gctggatctc cagccggcgg ttcgccttcc ccttgttgtc
     1441 ggttttcacg atccggagcc gtccgatttc gcgcgtgcgc gcgacgtgcg tgcccccgtc
     1501 ggcctgggca tcgtagccct cgatctccac cacccggatc ctctccacgt ccctcgggac
     1561 gagatccctg gccagctgga tgaggtccga tcgctcgaac tcctcccgag aaacgaaccg
     1621 gaccaggatg cgccggtcct cggcgatcac gcggttggtt tcctgctcga tcgcggccac
     1681 gcgctctttg ctgaggtcct cgagcgagaa gtccatccgg gcgcgatcag ggtagatcgc
     1741 cccgccggtc acccgcgcgc tgaacagccg gtacacggcc ccaaccagca cgtgcagggc
     1801 ggagtggtgc cgcatgatcg cgtaccgccg gtcccagtcc agcaccccgc gcacgtgcgc
     1861 gcccgctggc ggggtggggc cgtccagcac gtgccacacc gtctcgccgt cccggcgcat
     1921 ctcgccgacg cggctctccc ctcccggcca gcgcagccag cccgtgtcgg ccggctgacc
     1981 gccgccgccc ggatagaaga ccgtgccatc cagcgcgacg gcgttgtcct ccaccgcggc
     2041 gacggtggca tcggattccc tcagatagct gtcgcgcatg tacagcagct cggtcatcgg
     2101 gctcctccgc tgcccgcccc cgtcaacgta tccttggtaa cttgtgtgtc ttcacccact
     2161 cgatctgcgc agtgtggtga cgggcgtgcc aaacatagta ggcgagggcg cgtcccagcg
     2221 gcacaatctc ctgggtttcc ggatggaaga atgctcgctc aaacgcatcg ggcgcaagcg
     2281 accgaagcag gtgagcccac cgggcgtgca gcccttccag gagccgtaag gatggctcga
     2341 cgtctgcacg ctgcgcgtcg gccaggagcg accaccggct ctcgtcatag ggcttgatcg
     2401 tcggacgctg ttcggtgagc gccagcttga agcgcacgta gctgttgagg tggctatccg
     2461 cgaggtggtg cacgatctga cggatcgtcc agttccggta cctggtgtcg agatgctcgc
     2521 ccgacaggcc cgacaccacc tcacgcagcc gcgccggcac acgctggatc tcgtcgatcc
     2581 atagctcacg tcgccggtcg tcgtgcgctt cgtccgcgac gaatggcccg gccggaaact
     2641 gcggcggttc cggcaatacc ctgttggtca tcctgtcccc tc
//
LOCUS       DASQGH010000172         6623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51638, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000172 DASQGH010000000
VERSION     DASQGH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51638"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /gene="truB"
                     /locus_tag="VEW91_03220"
     CDS             complement(<1..648)
                     /gene="truB"
                     /locus_tag="VEW91_03220"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYM90630.1"
                     /translation="MDGVLVILKPPGMTSHDVVDLVRRAIGLRRVGHTGTLDPGAAGV
                     LVCCLGRATRLSEFLMEADKEYRVELRWGTRTSTGDAYGEVLPAPEPSAGRRPLTRPA
                     LEAVLKRFTGEILQVPPLVSAIHHEGTRLYELARRGETVDLQPRPITVHRIEILRFDH
                     THASAFLEVVCGKGTYIRKLCADIGEVLGTGGYAHFMVRTRAGRFGIRDAMTLEEL"
     gene            complement(654..1622)
                     /locus_tag="VEW91_03225"
     CDS             complement(654..1622)
                     /locus_tag="VEW91_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HYM90631.1"
                     /translation="MSPTETVAETLKSRRNILLLNHEGPDGDCLGSTLALARALWARG
                     QRATVGSADGVPEMYRFLPSSDRVLTVIPEDEAFDAVVFMECSAPERAGALAARAAGV
                     PLVVNIDHHVSNTGYGTLVVWDVDAAAVAEVAYPIVRALHPRLDADTATCLLTALLTD
                     TGSFHYASVTPRSFRLAADLVTAGASPASVYSQVYENRSVPSVRLLGTALSRMTLCED
                     GRVAWTTVTQAMLRETSAVMEDSEGIVGALRAIRGIRVAVLFKEEAEGIHVSLRGLGV
                     RAHVIAEAFGGGGHAAAAGFTFKAPLDEVIRQTLGAVHRELGATAR"
     gene            complement(1576..1980)
                     /gene="rbfA"
                     /locus_tag="VEW91_03230"
     CDS             complement(1576..1980)
                     /gene="rbfA"
                     /locus_tag="VEW91_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYM90632.1"
                     /translation="MSMRLARLRELFKEEVSTILQREMKDPRIGFVSITDVEVSADLR
                     HAKIFVSILGDADAKAKTMGALGNAQGFIRKELGRRIRLRRIPELHFRLDESIERGAR
                     VQRLLRRVAEGESEGRSDVPDGDRRGDAQEPA"
     gene            complement(1990..2271)
                     /locus_tag="VEW91_03235"
     CDS             complement(1990..2271)
                     /locus_tag="VEW91_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HYM90633.1"
                     /translation="MVVGVLRVECSLPGTRSIKDKRRILKSLLDRLHHRFHVAAAEVE
                     HQDSWRRAGLAVACVSTDTRHVDQVLAHVADAIGRHGDLIVLDYAVEMR"
     gene            complement(2289..4859)
                     /gene="infB"
                     /locus_tag="VEW91_03240"
     CDS             complement(2289..4859)
                     /gene="infB"
                     /locus_tag="VEW91_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYM90634.1"
                     /translation="MKVHELARELGLTSKELIEKLEAMKVPVRNHMTPLDVEVVDRVR
                     RTVAGGRDVAPPSGSPAPGTMGRDAVRGKATSVDAEKVRQSAFLQASYGSEIKGVRVI
                     RAPRAEAAPVAEPPSKPQAPPAKPEKASLGPAAPAAKVAGKTAERGAKRPAEAKPEAK
                     KAPVEPKPHADRAAREHPAAVPSPVAPAQAAAAPSAPPKEKPTIRFGDVRRTHRYEAA
                     GPEESGAPRLAPEPAPPPLPVEPARPVEPPRPAPGAPAVPAAPVMPAAPQPLRYLELD
                     GPIQVGDLAARMGAAPGEVVKRLVESGVMAGINHMIPLEVATKVMAAFNFAPRAPEVV
                     QVTELAPTLDVPAAEPVEKGREPRPPVVTVMGHVDHGKTSLLDAIRKTKVASLEFGGI
                     TQHIGAYTVQARGKPITFLDTPGHEAFTALRARGAHVTDIAVLVVAADDGVMPQTIEA
                     INHAKAARVPIIVAINKIDLPQANPDRVKQQLADLGLVPEEWGGDTITAPVSARQGTG
                     LDSLLEMILLVAELRDLRATPEKAARATVIEARLDKGRGPVATVLVQDGMLHMGDAVV
                     VGETYGRIRAMTDDKGARIQDAGPSMPVEVIGLSEVPAAGDLVEVVTNERIAKAVAEE
                     RTERRRATEQAKTRQLSVEEISKEAGAGIERKELRLIVKADVQGSVEALVAALERLTS
                     REVIITILHAAVGNVTESDIMLATASVARVIGFNVRPEPQVRRLAEAHGVDVRLYRVI
                     YEVLDDIKKLASGLLAPEMVEVILGRVEVRKVYSISRVGTIAGCYVTLGRMVRGARVR
                     LIRDGAVVHEGTIASLRRFKEDVREVLEGYECGIGLERYNDVKEGDVIEAFAVEAKPV
                     "
     gene            complement(4856..5236)
                     /locus_tag="VEW91_03245"
     CDS             complement(4856..5236)
                     /locus_tag="VEW91_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HYM90635.1"
                     /translation="MPKTRRLPQRQCVACGQMRAKRDLVRIVRTPGGEIRVDPTGKLS
                     GRGAYVCPEAACVDRALREERLARTLDRAIPEDVAVGLRGALTRPAASRAPVVRRVML
                     SRPASPSGRAPRGGPRGEYTGGMP"
     gene            complement(5240..6499)
                     /gene="nusA"
                     /locus_tag="VEW91_03250"
     CDS             complement(5240..6499)
                     /gene="nusA"
                     /locus_tag="VEW91_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYM90636.1"
                     /translation="MNNAELIKAIKQLEEEKGLSKDVLVEAIEAALLSAYKKNFGSAA
                     QNIRIELDRETGEQHVFSVRTIVETVTDPNTEVPLAEAQGWDTESQVGDIVEVEVTPK
                     DFGRIAAQTAKQVIVQRIREAERDMVYKEFRDREGDIVTGTVQRVERRSVYMDLGRIE
                     AVLPPTEQVPREGYRQNDRVKAYVVEVKQGTRGPQIVVSRTHPGLLKRLFEIEVPEIY
                     EGIVEIKAIAREAGARSKVAVLSRDRNVDAVGSCVGPKGSRVQSIVDELRGEKIDIVP
                     WASDSAIFVAGALSPAKVIRVEIADETKTALVTVPDNQLSLAIGKEGQNARLAAKLTG
                     WRIDIKSESQIKEQEAKKIFADLPVEEEPPAPAPAAVEAEPAPAAVETEPAPVAAEAE
                     PAPVGPPADGALIAEGAPRVEEKTIAE"
BASE COUNT          886 a         2474 c         2176 g         1087 t
ORIGIN      
        1 caactcctcg agcgtcatcg cgtcccggat cccgaaccgg ccggcccggg tgcgcaccat
       61 gaagtgcgcg tatccgccgg tccccaggac ctcgccgatg tccgcgcaca gcttgcggat
      121 gtacgtgcct ttgccgcaga ccacctccag gaatgccgac gcatgggtgt ggtcgaaccg
      181 gaggatctcg atgcggtgca cggtgatcgg gcggggctgg agatccaccg tctcgccgcg
      241 cctcgcgagc tcgtacagcc gggtgccctc atggtgaatg gcggacacga gcggcgggac
      301 ctggaggatc tctccggtga accgcttgag gacggcttcg agcgcggggc gcgtgagggg
      361 gcggcgaccg gcggacggct cgggggccgg gagcacttcg ccgtacgcat ccccggtgct
      421 cgtccgggtc ccccatcgga gctccacccg gtactccttg tccgcctcca tcaggaactc
      481 gctcaaccgc gtggcccggc cgaggcagca cacgagcacg ccggccgcgc ccgggtcgag
      541 cgtcccggtg tggcccacgc gccggaggcc gatcgcccgt cggacgagat cgacgacatc
      601 gtgcgaggtc atccccggag gcttgaggat aacgagaacg ccgtccatca cgctcagcgg
      661 gcggtcgctc ccagttcccg gtggacggcg ccgagggtct ggcggatcac ctcgtcgagc
      721 ggcgccttga acgtgaaccc ggccgccgcc gcatgcccgc cgcccccgaa cgcctcggcg
      781 atcacatggg cccggacgcc cagcccgcgg aggctgacgt ggatcccctc ggcttcttcc
      841 ttaaagagga ccgccacgcg aatcccgcgg atcgcgcgca gggctccgac gatcccctcg
      901 gagtcctcca tcaccgcgct cgtctcccgg agcatcgcct gggtcacggt ggtccacgcg
      961 acccggccgt cctcgcacag ggtcatccgg ctcagcgccg tgcccagcag gcggaccgac
     1021 ggaaccgacc ggttctcgta cacctggctg tacacgctcg cggggctggc ccccgcggtc
     1081 accaggtccg ccgcgagccg gaaactccgt ggggtgacgc tggcatagtg aaagctgccg
     1141 gtgtcggtca acagcgccgt cagcaggcag gtggcggtgt cggcgtcgag gcgcggatgc
     1201 agcgccctga cgatggggta cgccacctcc gcgacagcgg cggcgtcgac atcccagacc
     1261 acgagggtcc cgtatcccgt gttgctaacg tggtggtcga tgttcaccac cagcggcacc
     1321 ccggcggcgc gcgcggcgag cgccccggcc cgttcggggg cgctgcactc catgaagacc
     1381 accgcgtcaa acgcctcgtc ctccgggatc acggtgagca cccggtcgct cgaaggaaga
     1441 aaccggtaca tctccgggac gccgtcggcg ctgccgaccg tggcgcgctg tccccgcgcc
     1501 cacaacgctc tcgccagcgc cagcgtggac cccaggcagt cgccgtccgg cccctcgtgg
     1561 ttgaggagga ggatgttacg ccggctcttg agcgtctccg cgacggtctc cgtcggggac
     1621 atcgctccgc ccctcgcttt caccctcggc cacccggcgc agcagccgct gcacgcgcgc
     1681 gccgcgctcg atcgattcgt ccagtctgaa atggagctcg gggatgcgcc gaagccggat
     1741 ccgccggccc agttccttcc ggatgaaccc ttgggcgttg cccagcgcgc ccatcgtctt
     1801 cgccttggcg tcggcgtcgc cgaggatgct gacgaagatc ttggcatgcc gcaggtccgc
     1861 gctcacctcc acatcggtga tggagacgaa gccgatccgc gggtccttca tctcccgctg
     1921 gagaatcgtg ctgacctctt ccttgaagag ctcacggagc cgggcgaggc gcatgctcat
     1981 gggatgggct caccgcatct cgacggcata gtccagcacg atgaggtcgc cgtggcgccc
     2041 gatcgcgtcg gcgacgtggg cgagcacctg atcgacgtgc cgggtatcgg tgctgacgca
     2101 ggcgacggcc aggccggcgc ggcgccagga gtcctggtgc tccacctcgg ccgcggccac
     2161 atggaaccgg tggtggagac gatcgaggag cgacttcagg atccgtcgtt tgtccttgat
     2221 gctccgcgtg ccgggaaggc tacactcgac gcgcaggacg cccaccacca tcgtggacgc
     2281 tccccccctc agaccggttt ggcctcgacg gcgaacgcct cgatgacgtc cccctctttc
     2341 acgtcgttgt agcgctcgag gccgatgccg cactcgtagc cctcaagcac ctcccggacg
     2401 tcctccttga accggcgcag ggacgcgatc gtgccttcgt ggaccacggc gccgtcgcga
     2461 atcaagcgca cccgggcgcc ccggaccatc cgccccagcg tgacgtagca ccccgcgatc
     2521 gtccctacgc ggctgatcga gtagaccttg cgcacttcca cgcgccccag gatcacctcg
     2581 accatctccg gggccaggag cccgctggcg agcttcttga tgtcgtcgag gacctcgtag
     2641 atgacccggt acaggcggac gtccaccccg tgggcctccg ctaggcgccg gacctggggc
     2701 tccggccgga cgttgaaccc gatcacccgc gcgacgctgg cggtggcgag catgatgtcc
     2761 gactccgtga cattccccac cgccgcgtgc agaatcgtga tgatgacctc gcggctcgtg
     2821 agccgctcca gggcggcgac gagcgcttcc accgatccct gcacgtcggc tttgacgatg
     2881 aggcggagct ccttgcgctc gatcccggcc cccgcctcct tggagatctc ctccacgctg
     2941 agctgacggg tcttcgcctg ctccgtcgcc cggcggcgct cggtccgttc ctccgcgacc
     3001 gccttggcga tccgctcgtt cgtgacgacc tcgacgaggt ctcccgcggc cggcacttcg
     3061 ctgagcccga tcacttcgac cggcatgctg gggccggcgt cctgaatccg ggcacccttg
     3121 tcgtcggtca tcgcccggat ccgcccgtag gtctcgccca cgaccaccgc atcgcccatg
     3181 tgcagcatcc catcctggac gagcacggtg gccaccgggc cccgcccctt atccagccgg
     3241 gcttcgatga ccgtggcccg ggcggccttt tccggggtgg cccggagatc ccgcagttcc
     3301 gccaccagga ggatcatttc caacagcgag tccagcccgg tcccctgcct cgccgacacg
     3361 ggagcggtga tcgtatcgcc cccccactcc tcgggcacga gcccgagatc cgccagttgc
     3421 tgcttgacgc ggtccgggtt cgcctggggg aggtcgatct tgttgatcgc gacgatgatc
     3481 ggcacccgcg cggccttggc atgattgatt gcctcgatcg tttggggcat gaccccgtcg
     3541 tcggcggcaa ccaccagcac cgcgatatca gtgacgtggg ccccccgggc gcgcagcgcg
     3601 gtgaaggcct cgtgcccagg ggtgtccaga aacgtgatcg gtttgccccg ggcctgcacc
     3661 gtgtacgcgc cgatgtgctg ggtgatccct ccgaactcca gggacgccac cttggtcttc
     3721 cggatggcgt cgagcaggct cgtcttcccg tggtccacgt gccccatcac cgtcacgacg
     3781 gggggccggg gctcgcgccc cttctccacc ggctcggcgg cgggcacatc gagcgtgggg
     3841 gccagctccg tcacctgcac gacctccggc gcacgcggcg cgaaattgaa tgccgccatc
     3901 accttggtcg ctacctcgag cgggatcatg tgattgatgc ccgccatcac gcccgactcg
     3961 accagacgct tcacaacctc ccccggagcc gcgcccatcc gggcggcgag gtcgccgacc
     4021 tggatcggcc cgtccagctc gagataccgg agcggttggg gagccgccgg catgaccggg
     4081 gccgccggga cggcgggcgc cccgggcgcc ggacgcggag gctcgaccgg cctcgcgggc
     4141 tcgaccggca acgggggggg tgcaggctcg ggcgccaacc gcggcgctcc ggactcctcg
     4201 gggcccgccg cctcataccg gtgggttctc cggacgtccc cgaaccggat ggtgggcttc
     4261 tccttcggag gcgccgacgg ggccgccgca gcttgcgccg gtgcgacggg cgaagggacc
     4321 gcggccgggt gctccctggc cgcccggtcc gcgtggggct tcggctctac gggcgctttc
     4381 ttggcttcgg gcttcgcttc ggcgggccgc ttggcgccgc gctccgcggt cttgccggcc
     4441 accttcgcgg ccggggccgc cggcccaagg gacgccttct cgggcttggc aggaggcgcc
     4501 tgcggcttgg acgggggctc cgccaccggg gccgcctccg cgcggggggc gcggatgacg
     4561 cgaacgcctt tgatctccga gccgtagctg gcctggagga aggcgctctg ccggaccttc
     4621 tccgcgtcca ccgacgtggc cttgccgcgg accgcgtcac gacccatcgt gccgggcgcc
     4681 ggcgacccgg aagggggcgc cacgtcccgc ccgcccgcca cggtcctccg cacccggtcg
     4741 acgacctcga cgtcgagggg ggtcatgtgg tttcgcaccg gaaccttcat cgcctccagc
     4801 ttctcgatca actccttgct ggtcaggccg agttcccggg cgagttcatg cactttcatg
     4861 gcatccctcc cgtgtactca ccgcgcgggc ccccccgagg ggccctgccg gagggggacg
     4921 cggggcggga cagcatgacg cggcgcacga ccggcgctcg ggaggccgcc gggcgcgtca
     4981 aggcgccgcg caatcccacc gcgacatcct cgggaatcgc ccgatcgagc gtccgcgcca
     5041 gccgttcctc gcgcaacgcc cggtccacac acgccgcttc ggggcacaca tacgccccgc
     5101 ggccagacag cttcccggtc ggatcgacgc ggatctcgcc cccgggcgtg cggacgatcc
     5161 gcaccaagtc ccgtttggcg cgcatttggc cgcacgccac gcactgccgt tgggggagcc
     5221 gtcgtgtctt tggcatggcc tactcggcga tcgtcttctc ctcgactcgg ggcgcaccct
     5281 ctgcgatgag ggccccgtcg gcggggggcc cgaccggggc cggctccgcc tccgcagcga
     5341 ccggggccgg ctccgtctcc acagcggccg gggccggctc cgcctccaca gcggccggcg
     5401 cgggggcggg gggctcctct tcgaccggca ggtcggcaaa gatcttcttc gcctcctgct
     5461 ccttgatctg cgactcgctc ttgatatcga tgcgccagcc ggtcagtttg gccgccagcc
     5521 gggcgttctg cccttccttg ccgatcgcga gcgagagctg gttgtccggc acggtcacga
     5581 gcgcggtctt cgtctcgtcc gcaatctcga cccggatgac tttggccggg cttagggcgc
     5641 ccgccacgaa gatcgccgaa tcgctcgccc aggggacgat gtcgatcttc tccccgcgca
     5701 gctcatccac gatcgactgc acccgggagc ccttcggccc cacgcatgag cccacggcat
     5761 cgacgttgcg atcccgggag aggaccgcca ctttgctgcg cgcgccggcc tcgcgcgcga
     5821 tcgccttgat ctctacgatg ccttcgtaga tctccggcac ctcgatctcg aacagccgct
     5881 tcagcaatcc ggggtgcgtc cgcgagacga cgatctgggg cccccgggtg ccctgcttca
     5941 cctcgaccac gtacgccttc acccggtcgt tctgccgata gccctcgcgc ggcacctgct
     6001 cggtcggcgg gagcaccgcc tcgatccgtc cgaggtccat gtacacgctc cgccgctcga
     6061 cgcgctggac ggtgcctgta acgatgtccc cctcccggtc gcggaactcc ttgtagacca
     6121 tatcccgctc ggcctcgcgg atccgctgca cgatcacctg cttcgccgtc tgggcggcga
     6181 tccggccgaa gtccttgggt gtcacctcga cctcgacgat gtcgccgacc tggctttccg
     6241 tgtcccaccc ctgggcttcg gcgaggggga cctccgtgtt cggatcggtc accgtttcca
     6301 cgatcgtccg cacgctgaac acgtgctgct ccccggtctc ccggtccagt tcgatccgga
     6361 tgttctgagc ggccgagccg aagttctttt tgtaagcgga gaggagggcg gcctcgatcg
     6421 cctccacgag gacgtccttg ctcaggccct tctcctcttc tagctgcttg atcgccttga
     6481 tcagctccgc gttgttcact cgccgctcac ctccagcgct ccgcgagctc ctttggcggt
     6541 cgccctggat cgcttgaagt cttcgcgcaa ctccgccatg tccacgacga gccgggcttg
     6601 ggcgatctcc tcgtgcccca ggc
//
LOCUS       DASQGH010000173         5945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48849, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000173 DASQGH010000000
VERSION     DASQGH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48849"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEW91_03255"
     CDS             complement(<1..191)
                     /locus_tag="VEW91_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYM90637.1"
                     /translation="MRSLTIAATGDSLITMHVAHSQEPGFRALLDVLRGADVRFTNLE
                     VTLHEFRGSPQAASGGTYAA"
     gene            complement(206..1051)
                     /locus_tag="VEW91_03260"
     CDS             complement(206..1051)
                     /locus_tag="VEW91_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90638.1"
                     /translation="MGRFLRLLLRRRPMAAVALGLLVVTALGAVLAPWVGLPPPDRID
                     MGQRLHPPSGAHLFGTDNFGRDVFTRVVYSGRISLPIGLAVMALSTVLGTLVGLLIGY
                     YRRVDDVLTRILEGLMAFPAILLAIALVAALGFGARNEVIAIALVYFPRTARIVRAST
                     LQLKARAFVEAAVALGQGDARILAVHILRNALPPLIVQSTFVFAEAILADAALSFLGL
                     GVKPPAATWGNMLDEAHVFVGLAPWFIVFPGIAIVVTVFSLNLLGDAVRDLADPHAVA
                     GRGIG"
     gene            complement(1062..2003)
                     /locus_tag="VEW91_03265"
     CDS             complement(1062..2003)
                     /locus_tag="VEW91_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90639.1"
                     /translation="MSRYVAGRLAALLPVLLGVGATAFLLLHMIPGDPASVMLGPDAS
                     AAQVAHLRRALGLDRPLFVQLALWFGRLLRGDLGDSIFLRQTVAQSIWQHLGPTVGLT
                     ALAELLAIGVAIPSGVLAAWKRNSRFDQAFMSGVLLGVSVPSFWMGLNLILVFAVAAR
                     WFPVAGYESPLRGVGPWLQHLVLPAVALAFTQAGLIARMARDATIEVLDEDFIRTARS
                     KGVAEGWVLINHAFRNSLIPTVTVIGTSLANLLSGAVVVESVFVMPGIGNLVIQSISR
                     RDYPVIEGVVLFVAVVYVLVNLLVDLLYAVIDPRIRY"
     gene            complement(2019..3617)
                     /locus_tag="VEW91_03270"
     CDS             complement(2019..3617)
                     /locus_tag="VEW91_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90640.1"
                     /translation="MRNAEGSVVSVRSRTLGIRLLSLGAMAALVLGVPGGRAHSAGPT
                     SGGVIHVATNAEPGTLDWTSSTATATRLVAWHVYETLFALDRNYDVRPMLAEGYSVSA
                     DGLHYTIRLRKGVAFHNGQPMTADDVVASLERWGRVSGGGRETFKFIKHVTKVDASTI
                     LIDLSRVFTPLIASIGDPKQAAIIMPKGIAEGAGDKPVNDYIGTGPYMFQKWEQGHQI
                     VLVRNPRYSSRSEDWGGIAGKKVAYADEIDFFPVRDDQVRLAGVSTGQYDLALELQSD
                     LYPQIKANPRLTVDVVKVFTWRGAVFNKARSPFSDPRMRLAVVYAIKPADLMAATGPK
                     EFWNLDPALFFPEQKTLYSTVGGDVYNHQNLEKAKALMREAGYSGQKIVLMATKDYTW
                     NYNLTQVLVPQLQAAGFAVDAQVYDWPTLLSRRAKKDLWDIFLTGFSPSFDPTGVIFF
                     ANAWPGFYESKAMDGFLSKWGETAAADTAARTALLDQIQAVFYKEVPVAKFGNEYGLE
                     VYDDHLHGYTSFFDVRFWNTWVTR"
     gene            complement(3657..4751)
                     /locus_tag="VEW91_03275"
     CDS             complement(3657..4751)
                     /locus_tag="VEW91_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYM90641.1"
                     /translation="MTDDRRTRHPFHMYEAVQGQPEAFARIVNRHDGAMDAFAARIAS
                     CERLFLVGTGTSHHAAQVGEHVLRAYGGDLAVQSYPAFDFALYGPRLGARDCVIGISH
                     RGNKNYTVQSLARARAAGCVTALITGEGNPEGRAAAEAVFQTVPQEQSSTHTISYVAS
                     IAALSILASHLGHRRTGARLLPKRVLSEEVPAALREALTREGEVAPWARAHVGRRRIW
                     LAGGGPSATTALEIALKIKEAAYLGAEGMPIETFLHGPFQCAEPEDLFVLIAPAGAAQ
                     ERVAELGGMAAEIGAAYLVVSDQAQHPLRAGAAGWCAVPAVPEPFTALTCLVPLQLFS
                     YHLALVRGTNPDGFRLDDPRFARARARIRL"
     gene            complement(4752..5327)
                     /gene="pdxT"
                     /locus_tag="VEW91_03280"
     CDS             complement(4752..5327)
                     /gene="pdxT"
                     /locus_tag="VEW91_03280"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HYM90642.1"
                     /translation="MKIGVLAIQGDVIEHRAVLRRLGVEEVEVRSPRDLADLDGLILP
                     GGESTTIGKLMVRYGLDRAIPDQAGRGMAIYGTCAGMILLARRAAGGEPPLLRLMDIS
                     VIRNAYGRQVDSFEVDLDIPVLGPPPLRAVFIRAPVMDHVGPGVEVLASLDGRPVLAR
                     EGRLLASSFHPELAPDDRVHRYFLSMVRTGK"
     gene            complement(5344..>5945)
                     /locus_tag="VEW91_03285"
     CDS             complement(5344..>5945)
                     /locus_tag="VEW91_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HYM90643.1"
                     /translation="LGVDFIDESEVLTPADERYHINKHLFKIPFVCGCRDLGEALRRI
                     GEGAAMIRTKGEAGTGNVVEAVRHMRAVMDGIRVLQATPEEELMTAAKELGAPYELVL
                     ETRSLGRLPVVNFSAGGLATPADAAMMMQLGCDGNFVGSGIFKSKDPARRAKAIVEAT
                     THYNDPDVIARVSEGIGEPMRGLDVRKLPEAELMQTRGW"
BASE COUNT         1004 a         2102 c         1988 g          851 t
ORIGIN      
        1 gcggcgtagg tgccgccgct cgcggcctgg ggagatcccc gaaactcatg gagcgtgacc
       61 tcgaggttgg tgaaccggac gtccgccccc cggaggacat cgagcaacgc ccgaaatccg
      121 ggctcctggg agtgcgccac gtgcatcgtg atgagcgagt cgccggtggc ggcgatggtc
      181 agcgaacgca ccagactaca cctccttatc cgatcccccg cccggccacc gcatgcgggt
      241 ccgccaggtc gcgcacggcg tcgcccagca ggttgagcga gaacacggtg accacgatgg
      301 cgatgccggg aaagacgatg aaccacggcg ccaggccgac aaagacgtgg gcttcatcca
      361 gcatgttgcc ccacgtggcc gccggcggct tgacccccag cccgaggaag gagagcgccg
      421 catcggcgag gatggcttcc gcgaacacaa acgtcgactg cacgatgagg ggcggcaggg
      481 cgtttctgag aatgtggacc gcgaggatgc gcgcgtcgcc ctggccgagc gccaccgccg
      541 cttcgacgaa ggcgcgggcc ttgagctgca gggttgaggc gcgcacgatc ctggcggtgc
      601 gcgggaagta cacgagcgcg atcgcgatga cctcgttgcg cgcgccgaac ccgagcgcgg
      661 ccaccagggc aatcgcgagg aggatcgcgg gaaaggccat caacccctcc aggatccgcg
      721 tcaggacgtc gtcgaccctc cgatagtacc cgatcagcag accgaccagc gtgccgagca
      781 ccgtgctgag cgccatcacg gcgagcccga tcggaagcga gatccgcccg ctgtagacga
      841 cccgcgtgaa cacgtcgcgc ccgaagttgt cggtgccaaa gaggtgggcg cccgagggcg
      901 ggtgcaggcg ctggcccatg tcgatccggt cgggcggggg caggcccacc cacggggcca
      961 ggacggcccc aagcgcggtc acgaccagga ggccgagcgc caccgccgcc atcggccggc
     1021 gacggagcag caaccgcagg aatcgcccca cgcgaccgcc cctagtaccg gattcgcgga
     1081 tcgatgaccg cgtagagcag gtccacgagc aggttgacca gcacgtagac gaccgcgacg
     1141 aacagcacca cgccctcgat cacggggtag tcgcggcggc tgatggactg gatgacgagg
     1201 ttgccgatgc ccggcatgac gaacaccgac tccaccacca cggcccccga caggaggttc
     1261 gccaggctcg tcccgatcac cgtgaccgtg gggatgagcg agttgcggaa cgcgtgattg
     1321 atcagcaccc acccctccgc cacgcctttg ctccgcgccg tccggatgaa gtcttcatcc
     1381 aggacctcga tcgtggcgtc ccgcgccata cgggcgatca gccccgcttg cgtgaatgcc
     1441 agggccacgg ccgggaggac cagatgctga agccacggcc cgaccccgcg gaggggtgac
     1501 tcgtacccgg ccaccgggaa ccagcgggcg gcgacggcga agaccaggat caggttcaac
     1561 cccatccaga acgacgggac cgagaccccg agcagcaccc cggacatgaa ggcctggtcg
     1621 aaccggctgt tccgcttcca cgccgccagc acgcccgatg ggatcgccac gccgatcgcc
     1681 aggagctccg ccagcgcggt gaggcccacc gtggggccga ggtgctgcca gatcgattgc
     1741 gccaccgtct ggcggaggaa gatcgagtcc cccaggtcgc cccgcagcaa cctgccgaac
     1801 cacagcgcga gttggacgaa gagcggccgg tcgagcccga gcgcccggcg caggtgggcc
     1861 acctgggcgg ccgacgcgtc gggcccgagc atcaccgagg ccggatcgcc cgggatcatg
     1921 tggagaagca ggaaggcggt cgcgccgacg ccgagcagca cgggcaggag cgcggcgagg
     1981 cgcccggcta cgtacctcga cacggagcct cctcttcgct accgcgtcac ccacgtgttc
     2041 cagaaccgga catcgaagaa actcgtgtag ccgtggaggt ggtcatcgta gacttccagg
     2101 ccgtactcgt tgccgaactt cgcgaccggc acttccttgt agaagacggc ctggatctgg
     2161 tccagcagcg ccgtgcgcgc tgcggtgtcg gcggcagcgg tctcccccca cttgctcaga
     2221 aagccgtcca tcgccttgct ctcgtagaat cccggccacg cattggcgaa aaagatcacg
     2281 cccgtggggt cgaacgacgg cgaaaacccg gtgaggaaga tgtcccagag gtccttcttc
     2341 gcccgccggc tcagcagcgt cggccagtcg tacacctgcg cgtcgacggc gaagccggcg
     2401 gcctggagct ggggcaccag cacctgggtc agattgtagt tccacgtgta gtctttcgtg
     2461 gccatgagca cgatcttctg gccgctgtag cccgcttctc tcatcaaggc cttggccttc
     2521 tcgaggttct ggtggttgta gacgtcgccg ccgacggtcg agtacagcgt cttctgctcg
     2581 gggaaaaaga gggccggatc cagattccag aactccttcg ggcccgtcgc agccatcaga
     2641 tccgctggct tgatcgcgta gaccaccgcc aggcgcatcc gcggatcgct gaagggagac
     2701 cgggccttgt tgaacaccgc cccccgccag gtgaacacct tgacgacgtc gacggtcaga
     2761 cgcggattgg ctttgatctg gggatagagg tcggactgca actccagggc gaggtcgtac
     2821 tgtccggtcg acacgccggc cagccgcacc tggtcgtcgc ggaccgggaa gaagtcgatc
     2881 tcgtccgcgt acgcgacctt cttccccgca atcccgcccc agtcctcaga gcgcgaactg
     2941 taccggggat tgcgcaccag cacgatctgg tgcccctgct cccacttctg gaacatgtac
     3001 ggacccgtgc cgatgtagtc gttgaccggt ttgtccccgg ccccctcggc gatgcccttg
     3061 ggcatgatga tcgcggcctg cttgggatcc ccgatgctgg cgatgagcgg ggtgaacacc
     3121 cgggagaggt cgatcaggat ggtggacgca tccaccttcg tgacgtgctt gatgaacttg
     3181 aaggtctcgc gtcccccacc gctcacccgg ccccagcgct ccagcgatgc cacgacatcg
     3241 tccgcggtca ttggctggcc gttgtggaag gcgacccctt tgcggaggcg aatcgtgtag
     3301 tgcagcccgt cggcgctgac cgagtagccc tccgccagca tcggtctgac atcgtagttc
     3361 cggtcgagcg cgaacagggt ctcgtagacg tgccacgcca ccaaccgggt cgccgtcgcg
     3421 gtcgacgacg tccaatccag cgtgcccggt tcggcgtttg tggccacatg gatcaccccg
     3481 ccggatgtgg gcccggcgct gtgggcgcgc ccgcccggga cccccagcac gagcgcggcc
     3541 atcgcaccca aggagaggag tcgaatcccc agcgtcctgc tgcgcacgga gaccacgctc
     3601 ccttcggcgt tgcgcattgg cgagaacttc gggaaggtgg ggccgggccc ctcgcgctac
     3661 agccggatcc gggcgcgcgc ccgggcgaac cggggatcgt cgaggcgaaa cccgtcgggg
     3721 ttcgtcccgc gcacgagcgc gaggtggtac gaaaagagct ggagcggcac aagacaggtg
     3781 agcgcggtaa acggctcggg aaccgccggg acggcgcacc accccgcagc gccggcccgc
     3841 agcggatgct gcgcctggtc gctgacgacg agatacgcgg ccccgatctc ggcggccatc
     3901 ccaccgagtt cggccacgcg ctcctgcgcg gcgcccgccg gagcgatcag gacgaacagg
     3961 tcctccggct cggcacactg gaacggcccg tgcaggaagg tctcgatcgg catgccctcg
     4021 gcccccagat acgcagcctc cttgatcttc aacgcgatct cgagggcggt cgtggcgctc
     4081 ggcccgccgc ctgcgagcca gatgcgccgg cggccgacgt gggcccgggc ccagggagcc
     4141 acctcgccct cccgggtgag cgcctcccgc agcgcggcgg gcacctcctc ggacagcacc
     4201 cgcttcggaa ggagccgtgc gccggtgcgg cggtggccga ggtggctcgc caggatcgac
     4261 agcgcggcga tcgatgcgac ataactgatg gtgtgcgtcg aagactgctc ctggggcacc
     4321 gtctggaaga cggcctcggc cgccgcccgc ccttcggggt tgccctcgcc ggtgatgagc
     4381 gcggtcacgc accctgcggc gcgggcgcgc gcgagcgact ggacggtgta gttcttgttc
     4441 ccccggtggc tgatcccgat cacgcagtcc cgggcgccga gccgcggccc gtacagcgcg
     4501 aagtcgaagg ccggatacga ttggacggcc aggtcccccc cgtaggcgcg cagcacgtgc
     4561 tcgccgacct gcgccgcatg gtgggaggtg ccggtcccga ccaggaacag gcgctcgcag
     4621 gatgcgatgc gcgccgcaaa cgcatccatc gccccgtcgt gacggttcac gatgcgggcg
     4681 aaggcctcgg gctggccctg gacggcctcg tacatgtgga aggggtggcg cgtccgcctg
     4741 tcgtcagtca ttcacttgcc cgtgcggacc atgctgagaa aataccgatg cacccggtcg
     4801 tcgggagcca gttcggggtg aaacgacgat gccaacaggc gcccctcgcg cgccagcacg
     4861 ggccggccgt ccaaggacgc cagcacctcg accccgggcc cgacgtggtc catcacgggc
     4921 gcccggatga acaccgcccg cagcggcggc ggcccaagca cggggatgtc cagatcgacc
     4981 tcgaacgagt cgacctgccg cccgtaggcg ttccgaatca cggagatgtc catcaggcgg
     5041 agcagcgggg gctctccgcc ggccgcccgg cgggccagca ggatcatgcc cgcgcacgtc
     5101 ccgtagatgg ccatcccccg cccggcctgg tcggggatcg cgcggtccag accgtaccgg
     5161 accatcaact tcccgatcgt cgtggactcg cccccgggga ggatgagccc gtccaggtcc
     5221 gcgagatctc gcggggaacg cacctcgacc tcctccacgc caagacggcg gaggacggcg
     5281 cggtgctcga tcacatcgcc ctggatcgcc agcacaccga tcttcatcgc tcgccgctcc
     5341 ccgctaccac ccccgcgtct gcatgagttc ggcctcgggg agcttgcgga cgtcgagccc
     5401 acgcatcggc tcgccgatgc cctcggagac ccgcgcgatc acatccggat cgttgtagtg
     5461 cgtggtcgcc tcgacgatcg ccttggcgcg cctggccgga tccttggact tgaagatgcc
     5521 cgagcccacg aagttcccgt cgcagcccag ctgcatcatc atcgccgcat ccgccggcgt
     5581 cgcgagccca cccgcggaga agttcacgac gggcagccgt cccaacgacc gcgtctcgag
     5641 caccagctcg tacggagccc ccagctcctt cgccgcggtc atcagctctt cttccggcgt
     5701 cgcctggagc acgcgaatcc catccatcac ggcccgcatg tggcggaccg cctcgacgac
     5761 attcccggtg cccgcctccc ctttcgtgcg gatcatcgcc gccccctcgc cgatccggcg
     5821 gagcgcttct ccgaggtcgc ggcagccgca cacgaacggg atcttgaaca ggtgcttgtt
     5881 gatgtgatac cgttcgtcgg cgggcgtgag cacctcgctc tcgtcgatga agtccacgcc
     5941 caggg
//
LOCUS       DASQGH010000174         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54542, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000174 DASQGH010000000
VERSION     DASQGH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54542"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1640)
                     /locus_tag="VEW91_03290"
     CDS             complement(39..1640)
                     /locus_tag="VEW91_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90644.1"
                     /translation="MRGFAGSSLGVWAVVLVLLCLVGVGAATGQQPRKGGTLVVVLDT
                     DPPTITTATTTSLSDEQVGVSMLDSLIWVDRSDQIRPHLAESWTVSPDGLAYTFHLRH
                     DVKWHDGQPFTSADVKFSFEQILAKYHPRAAGSFVHVKSIETPDPYTVVARMQEPYAP
                     FLHSLGMSDGPIVPKHLLEGTDIVKSGFLRRPVGTGPFKFQEWIPGDRIVVVRNTDYF
                     EPGLPYLDRIIFKEIPDVHARAVALETGEADYVFDSFLGQSDVPQLARDKGIKLEYDT
                     DFPLDDVLIFNVRKPPLSNVKVRQAITTAIARGVINHRVFFGLGAPGRSPIDSRFTWA
                     YNPQADYQRLYAYNPAVAGRLLDEAGYPVKTNGMRFTIRHTFDIRRPGQLPLAQLLRE
                     QLRKVGIDLELDGVERSVMLDRIYNKWDFDTTTQLYNSNGDPAIGLQRLYICREIRKA
                     PFTNGSGYCNPEVDQLFAKGASVVSQAERGTYYKQVQLILVRDVPTLVLNERRRVDAA
                     RIVVNGLWGTKIGYERLAPVWLSTP"
     gene            complement(1716..2501)
                     /locus_tag="VEW91_03295"
     CDS             complement(1716..2501)
                     /locus_tag="VEW91_03295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYM90645.1"
                     /translation="MARVTPGLRLDGVRKAVLSPGDGCLMQPAAPAQAYAELAARYGV
                     TVMERTPVSRIQRAAGGFELSHEGQTWHAEQIVIAANAFAGPLLEDLGIRLPSYPYPR
                     HVFSLAPAPPDLSPTMPIVVHQDEDLMLRHDGQIVVLICGLRERSTLVPDMTPEGFEV
                     VRDRVARRVDTQGAVVSPLWSGIRAMTPDRRALIGAVPGVAGAWCAIGFSGHGIMHAP
                     AVGLAMAEWLRTGRPRRFDLTPLAPDRLGVPAGPAPMLEPGHQ"
     gene            complement(2521..2847)
                     /locus_tag="VEW91_03300"
     CDS             complement(2521..2847)
                     /locus_tag="VEW91_03300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90646.1"
                     /translation="MNLRVAVIGGGVVGTSIAYHLGMLGERGVTLFERGRIGSGTTSR
                     AVGGLRTLFGHRLEAELSLYSLDAYLRLERASGRSFGFRRCGYLLLASGPDRVEALGR
                     GARHPR"
     gene            complement(2871..>4021)
                     /locus_tag="VEW91_03305"
     CDS             complement(2871..>4021)
                     /locus_tag="VEW91_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYM90647.1"
                     /translation="AARTSPTLAALVNGILGYYCDNEPRHTPSVVHILPAVVPAALSV
                     GEAEGASGSDFFKAVVLGIEVGCRVSQALDPKALYARGFHPTSIGGTFGAATAAGHLL
                     RLSGTAWRSALGLAAQQACGLLAWVDDATEHSRPLNPGLAARNGVAAATWAALGMGGP
                     PDVFEGKYEIFTAFSGVRRPEGLTDGLGSRLLITELAAKRHVSCGFLHGGLDALLDLM
                     SEHTLTIQDIDALDLYLATTAARVVDNNPIKSHHAQYVLAVAALNGRVTIEDILGDGV
                     AHPDVARLSHRITVVYDEELERLYPRQYSAIVVLRTRDGRRLERRVDWPRGWPENPMT
                     EAQWMDKYRTLTAYRCTPAQSARIAELTLALSELGDIRVLGDALSLRA"
BASE COUNT          646 a         1394 c         1339 g          642 t
ORIGIN      
        1 gaaccctggc ggccgccatg cccgctgtcc tgcccgcgtc atggggtcga gagccacacc
       61 ggcgctagcc gctcgtaccc gatcttcgtc ccccagaggc cgttgaccac gatgcgcgcc
      121 gcgtccacac gccggcgctc gttgaggacc agggtcggca cgtccctgac caggataagc
      181 tgcacctgtt tgtagtaggt tccacgctcg gcctgggaca ccacggaggc ccccttcgcg
      241 aagagctgat cgacctcggg gttgcagtag ccggagccgt tggtgaacgg cgccttgcgg
      301 atctcgcggc agatgtacag ccgctgcagg ccgatcgccg gatcgccgtt cgagttgtag
      361 agttgggtgg tcgtgtcaaa gtcccacttg ttgtagatcc ggtcaagcat cacgctacgc
      421 tcgaccccgt cgagctcgag gtcgatcccg accttgcgca gctgctccct gagcagctgc
      481 gcaaggggca gttggccggg acggcggatg tcaaaggtgt gccggatcgt gaagcgcatg
      541 ccgttcgtct tgaccggata ccccgcttcg tcgaggagtc tccctgccac cgcgggattg
      601 tacgcgtaca ggcgctggta atccgcctgc gggttgtacg cccaggtgaa acgactgtcg
      661 atcgggctgc gcccgggggc gcccaacccg aagaagaccc ggtgattgat caccccgcgg
      721 gcgatggccg tcgtgatggc ctggcgcacc tttacgttcg agaggggcgg cttgcgcacg
      781 ttaaagatca gcacgtcgtc cagcgggaaa tccgtgtcgt actccagctt gatgcccttg
      841 tcccgagcga gttgcgggac gtcgctctgt ccgaggaacg agtcgaacac gtaatccgct
      901 tcgccggtct ccagcgcgac ggcgcgggcg tgcacgtccg ggatctcctt gaagatgatc
      961 cggtccaggt agggcaggcc gggctcaaag tagtcggtgt tccggacgac gacgatgcgg
     1021 tcacccggga tccactcctg gaacttgaac ggacccgtgc ccaccggcct gcgaaggaag
     1081 ccgctcttca cgatatccgt gccctccagg agatgctttg gcacgatggg gccgtcggac
     1141 attcccaggc tgtggaggaa cggtgcgtac ggctcctgca tgcgggcaac gaccgtgtac
     1201 gggtccggag tctcaatgct cttgacgtgc acgaagctac cggcagcccg tggatgatac
     1261 ttggccagga tttgttcgaa ggagaacttg acgtcggcgg aggtgaacgg ctgcccatcg
     1321 tgccacttga cgtcatggcg caggtggaac gtgtaggcca gcccatccgg cgacaccgtc
     1381 caggattccg ccaggtgggg acgaatctgg tcggatctgt cgacccagat gaggctgtcg
     1441 agcatggaga cccccacctg ctcgtcgctc aggctcgtgg tggtggccgt cgtgatggtc
     1501 ggcggatctg tgtccaggac gacgaccagc gtgcctcctt tgcgcggctg ctggccggtc
     1561 gcggccccga cgccgacgag gcacagcagc accagcacga cggcccacac gcccagcgag
     1621 cttcccgcga atcctctcat cccggctgcc ctcctcttgc gtcgcgccgt tccctcgatc
     1681 ccgttcccgg cgcgcctgtc ctcgcccgta ctaggctatt gatggcccgg ctcgagcatg
     1741 ggcgccggcc cggctggcac ccccagacgg tccggcgcaa gcggcgtcag atcaaaccgc
     1801 cgcggccgcc ccgtgcgcaa ccactccgcc atggcgagcc cgaccgccgg tgcgtgcatg
     1861 atcccatgac cgctgaaccc gatcgcgcac cacgcgccgg ccacacccgg cacggcaccg
     1921 atcaacgcgc ggcgatctgg cgtcatcgcc cggatgccgc tccagagcgg cgatacgacg
     1981 gccccctggg tgtctacgcg ccgggccacg cgatcccgca cgacctcgaa tccctcgggc
     2041 gtcatgtccg ggaccagcgt gcttcgctcg cggagcccac agatcagcac gacgatctgg
     2101 ccgtcgtgcc gcagcatcaa gtcttcgtcc tggtgcacga cgatgggcat ggtcgggctc
     2161 aggtccggtg gtgcgggagc gagcgagaac acgtggcggg gataagggta gctcggcagg
     2221 cggatgccga gatcttcgag gaggggaccg gcaaaggcat ttgcggcaat gacgatctgt
     2281 tcggcgtgcc acgtctgccc ctcgtggctg agctcgaacc ccccggccgc acgctgaatc
     2341 cgggaaacgg gcgtccgctc catcaccgtc accccgtacc gggccgccag ctcggcgtag
     2401 gcctgtgccg gcgccgcggg ctgcatgaga cagccgtcgc cgggagacaa caccgccttg
     2461 cgcacaccat ccaggcggag cccgggggtc acgcgggcca cctgcgtctc ggacagctcc
     2521 tcaacgggga tgccgcgcgc cgcggccgag cgcttccacg cgatcaggac cggacgccag
     2581 caacaggtag ccgcaccggc ggaatccgaa cgaccgcccg ctggcgcgct cgagccggag
     2641 atacgcatcc aggctgtaca gcgagagttc cgcttcgagc cggtggccga acagcgtccg
     2701 cagtccccct accgcccggc tcgtggtgcc ggatccgatc ctcccccgct cgaaaagcgt
     2761 cacgccgcgt tcacccaaca tccccaggtg ataggcgatg ctggttccga ccacgccccc
     2821 gccgatgacg gcgaccctga gattcatcgc gggcctcccg cgtcctcgtg ctacgcgcgc
     2881 agcgacagcg cgtcgcccag cacgcgaatg tcgcccaatt ccgagagcgc cagcgtcagc
     2941 tccgcgatgc gcgccgactg cgcgggcgtg caccgatacg cggtcagcgt ccggtacttg
     3001 tccatccact gcgcctctgt catggggttc tcgggccacc cccgaggcca gtccacgcgc
     3061 cgctccagcc tgcgcccgtc ccgggtccgc aggaccacga tcgcgctgta ctgccgagga
     3121 tagagtcgtt ccagctcctc gtcgtagacg acagtgatgc ggtgagacaa ccgggcaacg
     3181 tcggggtgcg ccaccccgtc gccgaggatg tcttcgatcg tgacgcggcc gttgagcgcg
     3241 gccaccgcga gcacatactg ggcgtggtgc gacttgatcg ggttattgtc cacgacgcgg
     3301 gccgcggtgg tcgccaggta gaggtccaac gcatcgatgt cctggatcgt gagggtgtgc
     3361 tcgctcatga ggtcgaggag cgcgtcgagc ccgccgtgca gaaacccgca gctcacgtgg
     3421 cgctttgccg cgagctcggt gatcaggagg cgcgagccca gcccgtccgt taggccctcc
     3481 gggcgccgca ccccggagaa tgcggtgaag atttcgtact tgccctcgaa gacgtcgggc
     3541 ggtccgccca tgccgagggc cgcccacgta gctgcggcca ctccgttccg cgccgcgagc
     3601 cctgggttca gcggccgcga atgctccgta gcatcgtcca cccaggcgag cagcccgcag
     3661 gcctgctgtg cagcgaggcc cagagcgcta cgccaggcag tgccgctcag gcgcagcagg
     3721 tgacccgcgg ccgtcgcggc gccgaaggtg ccgccgatcg aggtggggtg gaacccgcgc
     3781 gcgtagaggg ccttgggatc gagggcctgg ctgacacgac agccgacctc aatccccagc
     3841 accaccgcct tgaagaagtc gctgccgctc gcaccttccg cctcgccgac ggacagcgct
     3901 gccggaacta ccgccggcag gatgtgcacg acgctgggcg tgtggcgcgg ctcgttgtca
     3961 cagtagtatc ccaggatccc attgaccagc gcggcgaggg ttggggaggt gcgggcggcc
     4021 a
//
LOCUS       DASQGH010000175         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_29169, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000175 DASQGH010000000
VERSION     DASQGH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29169"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VEW91_03310"
     CDS             <1..238
                     /locus_tag="VEW91_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90648.1"
                     /translation="RALLGGRGLAVEAATTSHIPQEFDDWVRRSGIPAQEAAALRASF
                     LTAPAEARTAFRIETKDGEVHFAWPEIILLGIKR"
     gene            complement(273..1901)
                     /locus_tag="VEW91_03315"
     CDS             complement(273..1901)
                     /locus_tag="VEW91_03315"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HYM90649.1"
                     /translation="MGRHTIGIDFGTESARAVLVDVADGRVLSTASFPYPHGVLDRTL
                     PGGLDRLPPDWALQHPDDWLRATEALLRAMAAAGSPEDVVGIGIDFTSCTVLPATADG
                     TPLCLLPELQREPHAWPKLWKHHAAQPYADRINAGADRPEGEFLALYGGKTSSEWMWA
                     KGWQMLAEAPPSVSRATVRWIEGGDWIVWQLTGAEVRSACQAGYKAHWQKDGGYPPDA
                     FWRALDPALAGLRDTLGDPRPVGTRAGGLAASWASRTGLRPGTAVAVALIDAHAAVPA
                     LGVHEAGHLVMIMGTSTCHLVLDARRRPVRGISGVVADGILPGWFGYEAGQASVGDIF
                     LWFVRTSVPGRYGETGVFERLEDEAARLTPGGSGLLALDWWNGCRTPLVDADLSGAIL
                     GLTLTTEPAEIYRALLESTVYGTRRIIETFEAGGVPILGVHACGGLAERARLLLQITA
                     DVTGRPISVARVPQASAVGAAIYAAAAATGETDMTGVVRRMGSTERVTYQPHAQAARV
                     YDGLYQDYLEIARHFGEGGSSVMKRLHQLRHTTT"
     gene            complement(1916..3331)
                     /locus_tag="VEW91_03320"
     CDS             complement(1916..3331)
                     /locus_tag="VEW91_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HYM90650.1"
                     /translation="MKRPKAGILTFSDGRPHVHQALLPLTRQFQERLAARLAAAGWEV
                     VTGREIVWTTDLAQREARYLAAQEVEAVVFNYGIWAWPHYSAIAAEFVPHPILLLSNV
                     NPQYPGLVAMLAAAGSLDQIGVFHGRISGDIGDDAVFQRVLRFLRPAMATNRLRGEVY
                     GLIGGRSMGMYTAVPALDQWRREFGVDIEHIDQFEIVRLANAVPDARADAGIAWLERH
                     GAAIHYDGKKLTPELLRRQLKSYLAVREILDEWRCSFCGIKGQPEMTNHFATMDVAEA
                     LLNDPYDWEGPHDPIVCATEADSDGALTMEIFKHISGSPVLFADVRHYDAADDVWDLC
                     NSGQHATYFAGRSFDPAVNLKRVNLYPADIYFPAGGASVMHIAAPGPVTLGRLARRRG
                     RYWLAIVPGEFVEFPPATAQAKAAATTAVWPHAFARFRVPPAAFLSEYDSNHIHGVYG
                     DYVDDLVWAAKILGIEYRVFA"
     gene            complement(3389..4300)
                     /locus_tag="VEW91_03325"
     CDS             complement(3389..4300)
                     /locus_tag="VEW91_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM90651.1"
                     /translation="MSARRIASGVVAPAAVPQPWVRWTLGREGVTVLWYAAALAAVVA
                     ALAPIVWMLSTSLKPEGQILSFTPHWIPQTFTLENFGLVLARYAFLRWIVNSVVVAVA
                     ATGIVVAIDAMAGYALARFTFPGRGLIYDTIISMLLVPIQVTVIPLFLIFAQVHMLDT
                     FQALILPTTANVTGVFLMRQFFLGIPAELEEAARIDGAGDLQVFSRVVLPLSRPVMAA
                     VAALTFVSSWNNFLWPLIAINSDRSRTLPVGIAQYMGAQAGTSGSAPAFGPPLASAVM
                     ATAPALLAFFLLQRYLTQGITTTGIKG"
     gene            complement(4297..5202)
                     /locus_tag="VEW91_03330"
     CDS             complement(4297..5202)
                     /locus_tag="VEW91_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM90652.1"
                     /translation="MNVLPARAHRHRLRGGGSPLTPYLFLLPYLVVFVLFRFGPVVGG
                     FLTSFTSWSIVGSPRFVGLANYRALAHDPLFYTSLNNTFYFLLLTAPTLIVLGFVLAL
                     LLNRPIAGRDAARVLIFSPYAMMSAVVGILWTWILDSHFGLLNYYLAKVHLGPIAWLS
                     SDAAAMPAIALTTVWWTVGYNMVIFLAGLQDIPPQLEESARIDGAGPWQVLWTITIPL
                     LRPTTFVVVMLTVINTFQVFDQVYVMTGGGPGTATLTLVQYLFFQAFQTFKLGYGSAI
                     AYVTFALLLVMAWLQQRLVPSEVTK"
     gene            complement(5261..>6173)
                     /locus_tag="VEW91_03335"
     CDS             complement(5261..>6173)
                     /locus_tag="VEW91_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM90653.1"
                     /translation="GTFYQGTQYALPIDMHMHAVYVNLDMWKQAGLDPNKLPTTGADF
                     VGAAKKLTIDAAGRHPDDPGFDPRTTTQYGVGMMNNHHGFYMWYALRAQQGDPFLSAD
                     LSRTVFSDAKSNTAWQWLQDLVFKDHVVPVGETNPYQDFVTKHVAMLIDGPWQISGLQ
                     KISDLHWDTTTFPRVFGAPAAWGSGHLFTIPKQSDKARQGAAVTLAAWLVRHSQQWAL
                     SGNLPALLSVRSSAAFRALPGRRGFLEAQPYEVMLPDVKQSAQLYSSAAPSPIVIAAQ
                     TVLVRGQPVAQVTRDLRARIDAILAGP"
BASE COUNT         1045 a         2105 c         2148 g          875 t
ORIGIN      
        1 tcgcgcgctg ttggggggcc gcgggctcgc cgtggaggcc gcgaccacgt cgcacattcc
       61 ccaggagttc gacgactggg tccggaggag cgggatcccg gcgcaggagg cggccgcgct
      121 ccgcgccagt ttcctcaccg ctccggccga ggcgcgcacg gcgttccgga tcgagacaaa
      181 ggacggcgag gtccacttcg cctggccgga gatcatcctc ctgggaatca agcggtgacc
      241 gcgagttgtg gcggccgcgc cggcgcgaag gcctacgtcg tggtgtgccg cagttgatgt
      301 aggcgcttca tcacgctgct gccgccctcg ccaaagtgcc gcgcgatctc taagtagtcc
      361 tgatacaacc cgtcgtagac gcgcgcggcc tgcgcgtgcg gctggtacgt aacgcgctcg
      421 gtgctcccca tccgccggac cacgccggtc atgtcggtct cgccggtggc cgcggcggcg
      481 gcgtagatcg ccgctccgac cgccgacgcc tgggggacac gggcgaccga gatgggcctg
      541 ccggtgacgt ccgcggtgat ctggagcaag agcctggccc gctcggcgag gccgccgcag
      601 gcgtgcaccc cgaggatcgg caccccgccg gcttcaaacg tctcgatgat gcggcgcgtg
      661 ccgtacaccg tcgactcgag cagcgcccga tagatctcgg cgggctcggt ggtgagcgtc
      721 aggccgagga tggccccgga gagatcggca tcgaccagcg gcgtgcggca cccattccac
      781 caatcgagcg cgagcaggcc cgacccaccc ggggtgagtc gggcggcctc gtcctcgagc
      841 cgctcgaaca cgccggtctc cccgtagcgc ccgggcacgc tcgtccggac gaaccacagg
      901 aagatgtccc ccaccgacgc ctggccggct tcatacccga accatcccgg caggataccg
      961 tcggccacca cgcccgagat ccccctgacg ggccggcgtc gcgcgtccaa cacgaggtga
     1021 caggttgacg tccccatgat catcacgagg tggccggcct cgtgcacgcc caacgccgga
     1081 acggcggcgt gggcgtcgat cagggcgacg gcgaccgcag tccccggcct gaggcccgtc
     1141 cgggaggccc atgaggccgc gaggccgcct gcgcgcgttc cgacgggccg gggatcgccc
     1201 agcgtgtcgc gcaggcccgc cagcgccgga tcgagcgccc gccagaacgc gtccggtgga
     1261 tagcccccgt ccttctgcca gtgcgccttg taccccgcct ggcaggcgct ccgcacctcg
     1321 gcaccggtca gctgccacac gatccagtcg ccgccctcga tccagcgcac ggtggcccga
     1381 gacacggacg gcggagcctc ggcgagcatc tgccagccct tggcccacat ccactcggag
     1441 gaggtcttgc ccccgtacag agccagaaac tccccctccg gtcgatccgc cccggcgttg
     1501 atccggtcgg cgtagggctg cgccgcgtga tgcttccaca gcttgggcca ggcatgcggc
     1561 tcgcgctgga gttccggcag gagacacaac ggggtcccgt cggccgtcgc cggcaggacg
     1621 gtgcacgacg tgaagtcgat cccgatgccc acgacgtcct cggggctgcc ggcggcggcc
     1681 atggcacgca gcagtgcctc ggttgcccgc agccagtcgt cgggatgctg cagcgcccag
     1741 tcgggaggca ggcgatcgag gccgcccggc agcgttcggt cgagcacacc gtgaggatac
     1801 ggaaacgacg cggtggacag cacccgccca tccgcgacat ccaccaggac ggcgcgcgcg
     1861 gactcggtgc cgaaatcgat gccgatggtg tggcgcccca tcccgccgcc tcccctcagg
     1921 caaatacccg gtactcgatc ccgaggatct tcgcggccca gacgaggtcg tcgacgtagt
     1981 cgccgtacac gccgtggata tggttgctgt cgtattcgga cagaaatgcc gcgggtggca
     2041 cgcggaagcg ggcaaacgcg tggggccaca cggcggtggt ggccgcggcc ttggcttgcg
     2101 ccgtggccgg gggaaactcc acgaactccc ccggcacgat cgccagccag taccggcccc
     2161 ggcgacgggc cagccggccg agcgtcacgg ggcccggcgc ggcgatgtgc atgaccgatg
     2221 ctccaccggc cgggaaatag atgtcggcgg gatacaggtt cacgcgcttc aggttgaccg
     2281 ccggatcgaa ggacctcccg gcaaaatagg tggcgtgctg tccggagttg cagagatccc
     2341 acacgtcgtc ggctgcgtcg tagtgccgca catcggcgaa caacaccggg ctccccgaga
     2401 tatgcttgaa gatctccatc gtcagcgcgc cgtcactgtc ggcctccgtg gcgcagacga
     2461 tcgggtcgtg cgggccttcc caatcgtagg ggtcgttgag cagcgcctcg gccacgtcca
     2521 tggtggcgaa gtggttcgtc atctcgggct gccccttgat tccgcagaag ctgcaccgcc
     2581 actcgtcgag gatctcacgg accgccaggt aggatttgag ctggcggcgc agcagctcag
     2641 gcgtcagctt cttgccgtcg tagtggatcg ccgccccgtg gcgctcgagc caggcgatgc
     2701 ccgcgtccgc gcgggcgtcg ggcacggcat tcgccaggcg cacgatctca aactggtcga
     2761 tgtgctcgat atccacgccg aactcgcgac gccactgatc gagcgccggc accgcggtgt
     2821 acatccccat cgaccgaccg ccgatgaggc cgtagacttc tccgcggagg cggttcgtcg
     2881 ccatcgccgg ccggaggaac cgcagcacgc gctggaacac cgcgtcgtcg ccgatgtcgc
     2941 ccgagatccg gccgtggaac acgccgatct gatccaggct gccggccgcc gccagcatcg
     3001 ccaccaatcc cgggtactgg gggttgacgt tgctcaggag gaggattgga tgaggcacaa
     3061 actcggccgc gatcgccgaa taatgcggcc acgcccagat gccgtagttg aagacgaccg
     3121 cctccacctc ttgggccgcg aggtagcggg cctcgcgctg cgcgaggtcg gtcgtccaga
     3181 cgatctcgcg accggtcacg acctcccacc ccgcggcggc gagccgggcg gccaggcgct
     3241 cctggaactg ccgggtgagt ggcagcaacg cctggtggac gtgggggcgg ccatcggaga
     3301 aggtcaggat tcccgccttc gggcgtttca tgcgtcacct ccgatgaggg taggccgggg
     3361 gctcaatcac cgcggcccac gtgccaggct atcccttgat gcccgtcgtc gtgatcccct
     3421 gcgtgagata gcgctgcagc aggaagaacg cgagcagcgc cggggccgtg gccatcaccg
     3481 cggacgcgag cggcggaccg aacgccgggg ccgacccgct cgtgccggcc tgcgcgccca
     3541 tgtactgcgc gatccccacc ggcagcgtcc gggaccggtc gctgttgatc gcgatcaacg
     3601 gccagagaaa gttattccac gagctgacga acgtgagcgc ggcgaccgcc gccatcaccg
     3661 ggcggctgag cggcagcacg acccgcgaaa agacctgcag atccccggcg ccgtcgatcc
     3721 gcgccgcctc ctcgagctcg gcggggattc cgaggaagaa ctgccgcatc agaaagacgc
     3781 cggtgacgtt ggcggtcgtc ggcaggatga gcgcctggaa cgtgtccagc atgtgcacct
     3841 gcgcgaagat caggaagagc gggatcaccg tcacctgaat cggcacgagc aacatcgaga
     3901 tgatcgtgtc gtagatcagg ccgcggccgg ggaaggtaaa ccgggccagg gcgtacccgg
     3961 ccatcgcatc gatcgcaacc acgatcccgg tcgcggcgac ggcgacgacc acgctgttga
     4021 cgatccagcg gaggaacgcg tacctggcga ggaccagccc aaagttctcg agggtgaacg
     4081 tctgcgggat ccagtggggc gtgaacgaca ggatctgccc ctcgggtttg agcgaggtgg
     4141 acagcatcca gacgatcggc gcgagggcgg cgacgacggc ggcgagcgcg gcggcgtacc
     4201 acaagaccgt gaccccctcc cggccgagcg tccatcggac ccagggctga gggaccgccg
     4261 cgggcgcaac cacgccgctt gcgatccggc gcgcgctcac ttggttacct ccgacggcac
     4321 caggcgctgc tgaagccacg ccatcaccaa gagcagggcg aacgtcacat aggcgatcgc
     4381 cgacccgtag ccgagcttga acgtctggaa cgcctggaag aacaggtact ggacgagcgt
     4441 gagggtcgcg gtgccgggcc cgccgccggt catgacgtac acctggtcga agacctggaa
     4501 ggtgttgatg accgtgagca tgaccacgac gaacgtcgtc ggacggagga gcgggatcgt
     4561 aatggtccac agcacctgcc aggggccggc gccgtcgatc cgggcggact cctcgagttg
     4621 cggcgggatg tcctgtagcc ccgcgaggaa gatcaccatg ttgtagccga ccgtccacca
     4681 caccgtcgtg agggcgatcg ccggcatggc cgcggcgtcg ctggacagcc acgcgatcgg
     4741 cccgagatgc accttcgcca ggtagtaatt gaggaggccg aagtggctgt cgaggatcca
     4801 ggtccagagg atgccgacga ccgccgacat catcgcgtac ggggagaaga tgagcacgcg
     4861 tgccgcgtcg cggccggcga tcggccggtt gagcagcagc gcgaggacga acccaagcac
     4921 gatcagcgtc ggcgcggtca gcaggaggaa gtagaacgtg ttattgagcg acgtgtagaa
     4981 cagcgggtcg tgggcgagcg cgcggtagtt ggcgaggccg acgaaccgcg gggagccgac
     5041 gatgctccac gacgtgaagc tcgtcagaaa gccgccgacg accggcccga accggaacag
     5101 cacgaacacg acgaggtagg ggagcaggaa cagatacggc gtgagcggcg acccaccgcc
     5161 ccggaggcgg tgcctgtggg cgcgcgccgg aagcacgttc atgatcgaat gggacggccg
     5221 cgggggccag cgccccccgc ggccgcgtta cgcacgccgg ctacggtccc gccaggatgg
     5281 cgtcgatgcg cgcccggagg tcacgcgtca cctgggcgac gggctggccc cgcacgagca
     5341 ccgtctgggc ggcgatgacg atcgggctgg gcgccgcaga cgagtacagc tgcgccgact
     5401 gcttgacgtc ggggagcatc acctcatagg gttgcgcttc caggaatccc cgccgccccg
     5461 ggagcgcgcg gaacgccgcc gagctgcgga cggacagcag cgcgggcaga ttcccggaca
     5521 gcgcccactg ctgcgagtgc cggaccagcc acgccgcgag ggtcaccgcg gccccctggc
     5581 gcgccttgtc gctctgctta ggaatcgtga acaggtgtcc gctgccccag gccgccggtg
     5641 ccccgaagac gcgcgggaac gtggtggtgt cccaatgcag atcgctgatc ttctggagcc
     5701 ccgagatctg ccacggaccg tcgatcagca tcgccacgtg cttagtgacg aagtcctgat
     5761 acgggttcgt ctcgcctacc gggaccacat gatctttgaa aacgagatcc tgtaaccact
     5821 gccaggccgt gttgctcttc gcgtcgctaa acaccgtgcg cgagaggtcc gccgacagga
     5881 acgggtcccc ctgctgggcg cggagggcgt accacatgta gaagccgtgg tggttgttca
     5941 tcatgccgac gccgtactgc gtggtcgtcc tgggatcgaa cccgggatcg tcgggatggc
     6001 ggccggcggc gtcgatcgtc agcttcttcg ccgcgccgac gaagtccgcc ccggtcgtcg
     6061 gaagcttgtt gggatccaga cccgcctgct tccacatgtc caggttgacg tagacggcgt
     6121 gcatgtgcat gtcgatcggg agcgcgtact gcgtcccctg atagaacgtc cct
//
LOCUS       DASQGH010000176         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14923, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000176 DASQGH010000000
VERSION     DASQGH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14923"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VEW91_03340"
     CDS             complement(<1..1341)
                     /locus_tag="VEW91_03340"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYM90654.1"
                     /translation="MTNTFSLSRRQFIRTAGAVVVGFAMPAAFRGFAGPAPATALPGV
                     APDPSQLDSWLAVAADGSVTIYTDKVELGMGVSTAFAQIVADELDVPVRAISVVLGDT
                     ATTPDQGGVGGSTSISVGANPIRQAAAEARRVLLGLASVRLAAPADQLQIHNGIVTRV
                     DDPAKTVSFGELIGGRRFDVALNTAGANYTLAVSGSARPKSPDQYKVVGTSVPRTDIP
                     PKAAGRFSYIVDVRVPGMLHGRVIRPPSLGAKLISVDAARGVPGLAKVVRKGNFLGVV
                     AETEWDAVQAAQKLEVRWSAAAPAWPSMTDLYATMWAMPVRERRVMVQTGDVEAALTS
                     AARTIEARYAWPFQSHAMMGPSCAVAEVHDGVATLWSSTQHPHQLQGGIAELLGLPVE
                     KVRVIWVEGAGSYGRSGTDDAAADAALLAQAVGRPVRVQWMRADETGWDPKGPAV"
     gene            complement(1356..1502)
                     /locus_tag="VEW91_03345"
     CDS             complement(1356..1502)
                     /locus_tag="VEW91_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYM90655.1"
                     /translation="MTAAAFLARTPNPTDGEIRAALSGLICRCGAHMRVFRAVKRAAA
                     LRQG"
BASE COUNT          238 a          534 c          512 g          225 t
ORIGIN      
        1 gacggccggt cctttcggat cccaacccgt ctcgtcggcc cgcatccact gaacccggac
       61 cggacgcccc acagcctgcg ccaggagcgc ggcatccgcg gccgcatcgt cggtgccgct
      121 gcgaccatac gagccggcgc cctccaccca gatgacgcgc accttctcga ccgggaggcc
      181 gagcagctcc gcgatccctc cctgcagttg atgcgggtgt tgagtgctcg accatagggt
      241 cgctaccccg tcatgaacct cggcgaccgc gcaggagggg cccatcatcg cgtgcgattg
      301 aaacggccag gcatagcggg cttcgatcgt ccgggctgcg gaggtcaggg ccgcctctac
      361 atctccggtc tgcaccatca ctcgccgctc gcggaccggc atcgcccaca tcgtggcata
      421 aaggtctgtc atcgacggcc aggcgggtgc cgcggcagac catcggacct cgagcttctg
      481 cgccgcctgg accgcgtccc actccgtctc cgccaccacc ccgaggaagt tccccttccg
      541 aacgaccttc gccagtccgg gaacgccccg ggcggcgtcg acgctgatca gttttgcgcc
      601 caaggagggc gggcgaatga cgcggccgtg gagcatgccg ggcacccgga cgtcgacgat
      661 ataggaaaac cggccggcgg ccttgggcgg gatatccgtc cgcggtaccg acgtgccgac
      721 gaccttgtac tggtccggag atttcgggcg cgcgctgccc gaaacggcga gggtgtagtt
      781 cgcgccggcg gtgttcaggg cgacgtcgaa tcggcgcccg ccgatcaact cgccgaacga
      841 cacggttttc gccgggtcgt ccaccctcgt cacgatcccg ttgtgaatct ggagttggtc
      901 ggccggcgcg gcgaggcgga ccgacgcaag cccgaggagc acgcggcgag cttccgccgc
      961 ggcctggcgg atcgggttcg caccgaccga gatcgaagta ctgcctccca cgccgccctg
     1021 atccggcgtt gtcgcagtgt ctccgaggac gacggagatc gcgcggaccg gcacatccag
     1081 ttcgtccgcc acgatttggg caaaggccgt tgagacgccc atccccagct cgaccttgtc
     1141 ggtgtagatc gtcacgctgc cgtccgccgc gaccgcgagc cacgaatcga gttgggacgg
     1201 atcgggggcg acgccgggga gcgccgtggc gggtgccggg ccggcaaaac cccgaaatgc
     1261 ggccggcatc gcaaacccga ccacgaccgc gccggccgtc cggatgaact gacggcgtga
     1321 gaggctgaac gtgttcgtca tggacgtctc ctgactcatc cctgtcgtag ggccgcggcc
     1381 cgcttcacgg cgcggaagac ccgcatgtgt gccccgcacc ggcagatcag gccggacagg
     1441 gccgcgcgga tctcgccgtc ggtcgggttg ggggtgcggg cgaggaacgc ggcggccgtc
     1501 atgatccag
//
LOCUS       DASQGH010000177         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3618, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000177 DASQGH010000000
VERSION     DASQGH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3618"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEW91_03350"
     CDS             <1..203
                     /locus_tag="VEW91_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HYM90656.1"
                     /translation="RAIATQPALLIADEPTGELDEATGREIGRLLQDLSRDGLAVVLV
                     THNPDLAALADRQVRMRDGMLA"
     gene            200..1708
                     /locus_tag="VEW91_03355"
     CDS             200..1708
                     /locus_tag="VEW91_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90657.1"
                     /translation="MRHPAWLLAWRSLWDRPRRTLLLLGGYGIGVGVMIALLSVSEAL
                     LTQARDRDLVAGGDLVLLPEGVDPAVLKVNAVTDLDFTIQQAEFIFREILNGPRFAPA
                     VLAAAPQIEARQVYVRGRGQVRAAVASAGIPSLDRAAHATGGVPGASDTDADRAWIAP
                     TRADFLDRLDRFHEPPPGLKGTWAEWDYVNFVDPATGAYGYLTLLAGGEGRGAVLLRL
                     RRPGHEVDDLALDAAILPGDLAFTSASQRLGPARVWNDGGRYRITVHDPRVQADLRLT
                     PDPGYYLPPTEIAGSTFISGYAVPVGHGWMDGQIRTARTALRLTHVPAYHDHNWGTWR
                     GITWEWGEAGGEGGAVLYGALHVAPQEGAASMGRPAVLFVWEAQHNGGKAAGGFLGAF
                     TVRAIRYAGWHPGPRVSGRRVSVPAAITVEGEAGPDRVRLEIKVRDVLASVAAQAGPT
                     EPSPTGRSQTPRIIFLQLRGEAQVRGTVDGRSVAFSGNAAAETFVRVERPQP"
BASE COUNT          309 a          671 c          697 g          293 t
ORIGIN      
        1 cccgggcgat cgcgacgcag cccgccctcc tcatcgccga tgagccgacc ggggagctcg
       61 acgaggcgac ggggcgggag atcggccgtc tgctccagga tctcagccgc gacgggctgg
      121 ccgtggtgct tgtgacgcac aacccggacc tcgcggcgct cgcggaccgc caggtgcgaa
      181 tgcgggatgg gatgctcgca tgaggcaccc ggcctggctc ctcgcatggc ggagcttgtg
      241 ggaccgccca cggcggacgc tcctgctgct cgggggctat gggatcggag tcggcgtgat
      301 gatcgccctg ctgtccgtga gcgaggcgct cctcacgcag gcccgcgatc gtgacctggt
      361 ggccggtggc gatctcgtgc tgctgcccga aggggtcgat ccggccgtcc tcaaggtcaa
      421 cgccgtgacc gacctggact tcacgattca gcaggcggag tttatcttcc gcgagatcct
      481 caacgggccg cggttcgccc cggcggtcct ggcggccgcg ccccagatcg aggcccggca
      541 ggtgtacgtc cggggacgcg ggcaggtccg ggccgcggtc gcctccgcgg ggattccgtc
      601 cctcgaccgg gccgcgcacg ccaccggggg ggtgccgggg gcgagcgaca ccgacgccga
      661 ccgcgcctgg atcgccccga cgcgcgccga cttcctggac cggctcgacc ggttccacga
      721 gcccccgccc gggctcaagg ggacatgggc ggagtgggac tacgtcaact tcgtcgatcc
      781 cgctaccggt gcgtacgggt acctcacgct cctcgccggc ggggaagggc ggggcgccgt
      841 cctcctgaga ctccggcgtc ccggccacga ggtcgatgat ctcgccctgg acgcggcgat
      901 cctcccgggg gacctcgcct tcacctcggc cagccagcgc ctcggccccg cccgcgtctg
      961 gaacgatggg gggcggtacc gcatcaccgt gcacgatccc cgggtccagg ccgatctccg
     1021 gctcacgccg gacccgggat actacctgcc cccgacggag atcgccggga gcaccttcat
     1081 ctccgggtat gccgtcccgg tcggccacgg atggatggac gggcagatcc gcaccgcccg
     1141 caccgcgctc cgcctcacgc acgttcccgc ctaccacgac cacaactggg ggacgtggcg
     1201 gggaatcacg tgggagtggg gggaagccgg cggggagggc ggggccgtgc tgtacggcgc
     1261 cctgcacgtg gcccctcagg aaggggcggc atcgatgggc cggccggcgg tgctctttgt
     1321 gtgggaggcc caacacaacg ggggcaaggc cgcgggaggg ttcctcggcg cgttcaccgt
     1381 ccgggcgatc cggtacgcgg ggtggcaccc cggaccccgg gtgtccggcc ggcgcgtgtc
     1441 cgtcccggcg gcgatcacgg tggaggggga ggccggtccg gaccgcgtgc gtcttgagat
     1501 caaggtacgc gacgtcctgg cgtcggtcgc cgcgcaagcg ggccccacgg agccctcgcc
     1561 gacaggtcga agccagaccc ccaggatcat ttttctgcaa ctccgcggcg aagcccaggt
     1621 ccgcgggacg gtcgatgggc gatcggtggc gttctccgga aacgccgcgg cggaaacgtt
     1681 cgtccgtgtg gagcgtccgc aaccctaaac tcagacagcg ccggtaaaag ccttcaaagg
     1741 cgactttgct agactccctc tgggcttctc aaaagccctt gattggcttg ataagagtcg
     1801 tctcatcaga tctggcagac aagtggatag gttgcgcgaa ggacgtccaa cccaaagtaa
     1861 tgtagcaggt gaagaagcgc gagtccgaga atcgtcgttc aaaagccagc gcctattcac
     1921 caataaccag ttttgagagg agagaatgcc atgaaagaat catccatcaa
//
LOCUS       DASQGH010000178         9969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54549, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000178 DASQGH010000000
VERSION     DASQGH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54549"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VEW91_03360"
     CDS             complement(<1..597)
                     /locus_tag="VEW91_03360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM90658.1"
                     /translation="MNKVVNSQDVVAEIHDGATVAIGGSSLSRKPMALVRALARSDRK
                     NLRLIVNVGGPEVDLLLGTGKVAELVYAFVGFEVMGLAPHFRRARQDGSVRFQEWTEF
                     TVMAGLDATIKRVPFLPTHTGLGTDLLRVNTAFRTIQDPFEGQTLLAVPALRPDVALI
                     HVNLADPQGNGVILGDGHMDVLCAKAARKTFLSAEQILL"
     gene            complement(594..1403)
                     /locus_tag="VEW91_03365"
     CDS             complement(594..1403)
                     /locus_tag="VEW91_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM90659.1"
                     /translation="MFTGTFTDKIALVTGASRGIGRATALTLAGGGADVVVHYHRNQA
                     MAEEVVEAAQGLGRRAMAVGANLEAPEEITRLFDQITERFGALDIFVANHAATAFKST
                     LEIKLHHNQRTFDLIVRSLVLCVQRAVPLMKGRNGAIVTIGGQGTAECLPNYALLGSA
                     KGALETWTRYIAYELAGEGITANCVSPGVIATDSARFYGGDRFAEFDRLVSAYTPRGR
                     MGTPEEVAAVIAFLCSPGARFVHGQTIVVDGGLGLVSAPFEMFHAQGEGRA"
     gene            complement(1440..2681)
                     /gene="fabF"
                     /locus_tag="VEW91_03370"
     CDS             complement(1440..2681)
                     /gene="fabF"
                     /locus_tag="VEW91_03370"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYM90660.1"
                     /translation="MARAVITGIGAITPVGLSAPETWASLLAGRSGVRPLDRFDASRF
                     PVRIAADVRGFDPLTVMTRKRARRTARFAQLAVAAAAEALADARLPSLGEISDRFGIS
                     IATALGGLDVIDAEAPRLYGQPPARVSPFLLPMLLANMASSAVSIQFGLRGPSSTPVG
                     ACASGTIALMEARRWVETGEADYVLAGGTEGGLTPTVWAALCALGALSTRNDAPEQAS
                     RPFDLDRDGFVYGEGAVVFVVERDDLARAREAQVYAELAGAAMTSDAYHETAPQPEGD
                     TAALAIRRALANAHAKPDEVDLIVAHGTGTPLNDVAETRAIKRALGQRARRIPITAPK
                     SMVGHLIGAAGSLAALVGVLAIRDGRIPPTINLDTPDPDCDLDYVPRAMREARVRVAL
                     ANAFGFGGQNCVVAVRAHVAR"
     gene            2911..4209
                     /gene="purD"
                     /locus_tag="VEW91_03375"
     CDS             2911..4209
                     /gene="purD"
                     /locus_tag="VEW91_03375"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYM90661.1"
                     /translation="MRVLVIGSGGREHAVSWKLHREGVRVYCAPGNPGIAEIAECLPL
                     SPQDPTALVESALANEIDLTFVGPEAPLAAGLVDHFAGRGLRVFGPTRAAAALESSKA
                     FMKRLCHRYGIPTAPFQIFDAPSLAAEHVRRAGRPLVIKADGLAAGKGVTVAPTPEEG
                     LAAVEALMIARRFGDAGARVVIEEALEGEEVSIFALCDGTAVSPLLPVQDHKRLLDGD
                     RGPNTGGMGAYAPVPLVSPNLLERITDEILEPTMWAMVQEGRPYRGVLFAGLMLTPEG
                     PQVLEFNVRLGDPEAQVLLPLLEGGLVEALDAVLSGGVERWAPRWHPGSAACVVLGSE
                     GYPESPRAGRAISGLTEAAACDGVLVFHAGTASREGGLVSAGGRVLNVVGWGPALQEA
                     RRRAYRAVEMVQYEGKVFRRDIGARGLPRGIIDASPVRAG"
     gene            4221..4769
                     /gene="purE"
                     /locus_tag="VEW91_03380"
     CDS             4221..4769
                     /gene="purE"
                     /locus_tag="VEW91_03380"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYM90662.1"
                     /translation="MGQPSGRPVVGIVMGSASDYDTMEQAARVLQEFGVPYEITIASA
                     HRSPARVEAYVKDAEGRGIRVIIAGAGGSAHLAGVLAGRTVLPVIGVPLAGVALGGLD
                     ALLSTVQMPGGVPVATVGIGGARNAALLAVQVLGTGDPALRERYRVYKERLAREVEEH
                     AARLATKAEPGMVPPHPGGGPR"
     gene            4769..6055
                     /locus_tag="VEW91_03385"
     CDS             4769..6055
                     /locus_tag="VEW91_03385"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYM90663.1"
                     /translation="MPVTAVIGAQWGDEGKGKVVDTLARRADLVIRYNGGNNAGHTIQ
                     NQYGTFRLHLLPSGIFHPAAQCIIGPGVVINPDVLLAEVADVEGVGMPTVGRLWLSDR
                     AHLIFPHHILIDELEEAVRGGSPHGTTKQGIWPVYSDKVGRVGIRLGDLLEEQHLVGQ
                     LTYLADRKSAVLRGVYRHEPVEAGQLLAACQRWAQRLRPYITDTHPLIQEALRADRVV
                     LLEGQLGVMRDLDWGLYPFVTSSTTLPGGASAGAGIPPYRITRVLGVAKAYTTAVGSG
                     PMPTELADATGERIRDLGQEFGATTRRPRRCGWFDGVAARFAAEVAGFTHLAVMKVDV
                     FDSFEQIRVAVAYRLDRRTVHTVPHTAAMARVEPIYEELPGWRCSTHGARRLGDLPIE
                     ARTFLRRIEEIVGVSIALVGVGPDREDMIYCQEGWA"
     gene            6052..7416
                     /gene="purB"
                     /locus_tag="VEW91_03390"
     CDS             6052..7416
                     /gene="purB"
                     /locus_tag="VEW91_03390"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYM90664.1"
                     /translation="MIPRYTSPEMGALWSPETKFATWLEIELLAAEAQSRLGRIPEAA
                     VRRLRERARVGSVARIDEIEERETRHDVVAFLRVVGETVGDDARYLHLGLGSSDVVDT
                     ALSVLMVRAADLIGASLGRLHRALAALAQKHRYTIMAGRTHGVHAEPVTFGLKAALWY
                     AEVGRGLDRIRRAREVIGVGKISGEVGTFAHTPPEVEAFVCERLGLQPAPASSQVIQR
                     DRHAEYLAHLAVVAGTLEKIATEIRQLARTELREVEEPFREGQTGSSAMPHKRNPVLC
                     ERVTGLSRVVRGHAVAALENIALWGERDITHSSAERVIIPDATSLLDYMARTLAGVIE
                     GLRVYPDHMRQNLDRSGGLVFSHRVLLALLDRGRSREEAYRIVQSAAMRAWEEQGGFR
                     ELLRASGGLSDAELDVCFDPSYALRHVDEIFARVGLGERPAVPAGGQADRESKARRGG
                     SADA"
     gene            7409..8323
                     /locus_tag="VEW91_03395"
     CDS             7409..8323
                     /locus_tag="VEW91_03395"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYM90665.1"
                     /translation="MHDVVADGQAGVVLETHLDLPLFSRGKVRDIYDLGDRLLVVATD
                     RLSAFDVVLPTGIPDKGRVLTGLSAFWFERLARRVPTHFLTADVNAFPPHLREPRAVL
                     EGRSMIVRKLRRIDIECVVRGYLAGSAWKEYAATGGVSGLVLPRGQRPGARLPAPVFT
                     PATKAASGHDENISEGRMAEIVGGALTRRLREASLAIYREAAQTAERCGLLLADTKFE
                     FGLDGDEIVLIDEVLTPDSSRFWDAAAYAATGSTESYDKQVVRDYLERVGWNKRPPAP
                     ALPPEVVGATRARYLEAYRRLTGRELSA"
     gene            8335..8613
                     /gene="purS"
                     /locus_tag="VEW91_03400"
     CDS             8335..8613
                     /gene="purS"
                     /locus_tag="VEW91_03400"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HYM90666.1"
                     /translation="MPTEQGAEQVRTRQARTVRIVVTLKPGVLDAPGQAIRQGLDALG
                     HRGVHSIRAGKYFEVDLADDGRIEERVREMCEGFLANTLIEEYRYEVL"
     gene            8626..9372
                     /gene="purQ"
                     /locus_tag="VEW91_03405"
     CDS             8626..9372
                     /gene="purQ"
                     /locus_tag="VEW91_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HYM90667.1"
                     /translation="MDVGIIVFPGSNCDADTYHVVRDVLGYRARYVWHEERQLAGLDA
                     VLLPGGFAYGDYLRAGAIAATSPVVRALRGYVEAGGLVLGICNGFQVLLEAGLLPGAM
                     RLNASGQFRCEFIHIRAERVDTPFTCAMTPGQVLRLPIAHAEGNYYVDAPTHQALVAG
                     RQIVFRYCDPGGTVTPQANPNGSYDAIAGGASPGGTVVGLMPHPERASEDLLGSADGR
                     LVFESLHRWVTAGGRPAPEAVAAATGAEAG"
     gene            9377..>9969
                     /locus_tag="VEW91_03410"
     CDS             9377..>9969
                     /locus_tag="VEW91_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HYM90668.1"
                     /translation="MQTVPVTDLAGTGLRPAEYDEICRRLGRAPNRVELAMFGVMWSE
                     HCAYKHSKGVLRRLPVRGRRVLAGPGENAGAVMVADGWAAVFKMESHNHPSAVAPFHG
                     AATGVGGIIRDILAMGARPIALLDSLRFGPPEDPGVAPLMRGVVAGIAAYGNSIGVPT
                     VGGELIHAPCYRANPLVNVACLGIVRADRLATARASGP"
BASE COUNT         1430 a         3422 c         3635 g         1482 t
ORIGIN      
        1 cagcaggatc tgctcggccg agaggaacgt cttccgggcc gcctttgcgc acaacacgtc
       61 catgtggccg tcccccagga tgacgccgtt gccctgcgga tcggccaggt tcacgtggat
      121 cagcgcgaca tccggtcgga gcgcgggcac ggcgagcagg gtttgcccct cgaacggatc
      181 ctggatcgtg cggaacgcgg tgttgacgcg gagcaggtcc gtcccgagcc cggtgtgggt
      241 cgggagaaac gggacccgtt tgatcgtggc atcgagcccg gccatgacgg tgaactcggt
      301 ccattcctga aagcggaccg atccgtcctg gcgggcgcgc cggaagtggg gcgccagccc
      361 catgacctcg aaccccacga aggcgtagac cagctcggcg accttgcccg tgccgagcag
      421 caggtcgact tccggtcccc ccacgttgac gatcagccgg aggttcttgc ggtcgctccg
      481 cgcgagcgcc cgaacgagcg ccatcggctt gcgcgagagg gaggaccccc cgatcgccac
      541 cgtggcgccg tcgtggatct ccgccacgac atcttggctg ttcaccacct tgttcatgcc
      601 cggccctccc cttgcgcgtg aaacatttcg aacggcgcgg acacgagccc aagcccgccg
      661 tcgaccacga tcgtctgccc gtgtacgaac cgggcgcccg ggctgcagag gaacgcgatc
      721 actgcggcga cctcttccgg ggtgcccatc cggccccgcg gggtatacgc ggaaaccagt
      781 cggtcgaact ctgcgaagcg gtccccgccg tagaatcggg ccgagtcggt cgcgatcacg
      841 cctgggctga cgcagttggc ggtgatccct tcgcccgcca gttcataggc gatgtagcgt
      901 gtccaggtct ccaacgcccc cttggcggag ccgagcaacg cgtagttggg caagcactcg
      961 gccgtcccct gcccgccgat cgtcacgatc gccccgttgc ggcccttcat gagggggacc
     1021 gcccgctgga cacacaggac cagggaccgc acgatgaggt caaacgtccg ctggttgtgg
     1081 tggagcttga tctccagggt ggacttgaac gccgtcgccg cgtggttggc gacgaagatg
     1141 tcgagcgccc caaaccgctc ggtgatctga tcgaagaggc gcgtgatctc ctcgggcgcc
     1201 tcgaggttcg cacccaccgc catcgcgcgc cgcccgagcc cctgggccgc ctccaccacc
     1261 tcctccgcca tggcctggtt gcggtggtag tgcaccacca catccgctcc tccgccggcc
     1321 agggtgaggg ccgtggcccg gccgatcccc ctcgaggctc ctgtgacgag cgcgatcttg
     1381 tccgtgaacg tcccagtaaa cactctggcg cctcctccca ggttgtgtgc ggagcgggcc
     1441 taccgggcta cgtgcgcccg gacggcgacc acgcagttct gtcctccgaa tccgaacgcg
     1501 ttggcgagcg cgacccgcac ccgggcctcc cgcatcgcgc ggggaacgta atcgaggtcg
     1561 cagtcgggat cgggggtgtc gaggttgatc gtcggcggga tccgcccatc ccggatggcc
     1621 agcacgccga cgagcgccgc gagcgacccc gcggccccga tcaggtggcc gaccatgctc
     1681 ttcggcgcgg tgatggggat cctgcgggcg cgctggccga gcgcgcgctt gatcgcgcgc
     1741 gtctccgcca cgtcgttcag cggggtgccg gtgccgtgcg ccacgatgag gtcgacctcg
     1801 tcgggcttgg cgtgcgcgtt ggccagcgcc cggcggatcg cgagcgccgc cgtatcgccc
     1861 tccggctgcg gggcggtctc gtggtaggca tcgctcgtca tcgccgcgcc ggccagctcc
     1921 gcatacacct gcgcctcccg cgcccgcgcg agatcgtcgc gttccacgac aaacaccacc
     1981 gcgccctcgc cgtacacgaa cccgtcccgg tccaggtcga acggacggct ggcctgctcc
     2041 ggggcgtcgt tccgcgtgct gagcgccccg agggcgcaca gggccgccca cacggtcggc
     2101 gtcaaccccc cttccgtgcc gccggcgagg acgtaatcgg cctctccggt ttcgacccac
     2161 ctccgcgcct ccatgagcgc gatcgttccg ctggcgcacg ccccgaccgg ggtgctggac
     2221 gggcctcgga ggccgaactg gatgctcacc gcggaggacg ccatattggc gaggagcatc
     2281 ggcaggagga acggcgacac gcgggccggg ggctggccgt agagccgggg ggcttcggca
     2341 tcgatgacgt cgaggccccc caaagccgtg gcgatcgaga tcccaaaccg atcgctgatc
     2401 tcccccaggg acggcaggcg ggcgtccgcg agggcctccg cggcggccgc gaccgcgagc
     2461 tgggcgaacc gggcggtccg gcgcgcgcgc ttccgggtca tcaccgtcag gggatcgaac
     2521 ccccggacat cggcggcgat gcggaccggg aaccgggacg cgtcgaaccg gtccagcggc
     2581 cggaccccgc tccggccagc cagcaggctg gcccaggttt caggggccga cagccccacg
     2641 ggcgtgatcg ctccaatccc cgtgataacg gctcgggcca cgaaacctcc acgtcgacgg
     2701 tgcccacctt ttcgccgcgg tttcaggggt ttcctatgca gcggcgcgcc gaatggcggc
     2761 acattcaagt taaaagacga acgatcaaac tcccgcttgg caatttcttt cgtctatgat
     2821 acagtgtcct tggacttcat cgctctttct acccagcagc ttaagaaaaa cccgaacaat
     2881 cttcggagcc caccgcgacg tggagcaacg atgagagttc tcgtgatcgg ctcggggggt
     2941 cgggagcacg ccgtctcgtg gaaactccat cgggagggcg tgcgagtcta ctgtgccccc
     3001 ggaaaccccg gaatcgccga gatcgccgaa tgccttccgc tgtcccccca ggaccccacc
     3061 gcactcgtcg agagcgcgct ggcgaacgag attgacctga cgttcgtcgg gccggaggct
     3121 ccgctcgccg cagggcttgt cgaccatttt gccgggcggg ggctccgggt gtttgggccc
     3181 acccgggccg ccgcggcgct cgagtcgagc aaggccttca tgaaacggct gtgccaccgg
     3241 tacgggatcc ccaccgctcc gttccagatc ttcgatgccc cgagtctggc tgccgagcat
     3301 gtgcggcggg cgggccgccc gctcgtgatc aaggccgacg gcctcgccgc cgggaagggc
     3361 gtcacggtgg cgccgacccc cgaggagggc ctggccgccg tggaggccct catgatcgcc
     3421 cggcggtttg gagacgccgg ggcccgcgtc gtgatcgagg aggcgcttga gggcgaagag
     3481 gtcagcatct tcgcgttatg cgacgggacc gccgtctcgc cgctgctccc ggtgcaggac
     3541 cacaagcggc tgctggacgg cgaccgcggt ccgaacaccg gtgggatggg cgcctacgct
     3601 ccggtccctt tggtctcgcc gaatctcctc gagcggatca ccgacgagat cctggagccg
     3661 acgatgtggg cgatggtgca ggaagggcga ccctaccggg gcgtgctgtt tgcgggcctg
     3721 atgctcaccc ccgaggggcc acaggtgctg gagttcaatg tcaggctggg ggatcccgag
     3781 gcgcaggtcc tgctcccgct cctcgagggc gggctcgtcg aggccctgga cgccgtgttg
     3841 agcggcggcg tcgagcggtg ggctccccgg tggcatcccg ggagcgcggc atgcgtggtg
     3901 ctcggctcgg agggctaccc cgaatcgccg cgcgccggtc gtgcgatcag cgggctcacc
     3961 gaagccgccg cctgcgacgg ggtcctcgtc tttcacgccg ggaccgcctc gcgcgaaggc
     4021 gggctggtgt ccgccggggg gcgcgtgctg aacgtggtcg ggtgggggcc ggcgctccag
     4081 gaggcgagac gccgggcgta ccgcgcggtc gagatggtgc agtatgaagg caaggtgttc
     4141 cgccgggaca tcggcgcgcg gggactcccc cgcggcatca tcgacgcctc gccggtgcgg
     4201 gccgggtgaa ggggacgatg atgggccaac cgtccgggcg ccccgtggtc ggcatcgtga
     4261 tggggagcgc cagcgactac gacacgatgg agcaggccgc gcgagtcctg caggagtttg
     4321 gcgtgccgta cgagatcacg atcgcctccg cccaccgctc gccggcgcgc gtggaggcct
     4381 acgtgaagga cgcggagggg cgggggattc gcgtgatcat cgccggggcg ggtggctcgg
     4441 cgcacctggc cggcgtgctc gccggaagga ccgtcctccc ggtcatcggt gtgccgctgg
     4501 ccggcgtggc tctgggaggg ctcgacgcgc tgctctcgac cgtccagatg cccggagggg
     4561 tgccggtcgc gaccgtgggg atcgggggcg cgagaaacgc ggccctgctc gccgtccaag
     4621 tcctcggtac cggcgatccg gcgttgcggg agcgttaccg ggtgtacaag gagcggctcg
     4681 cccgcgaagt cgaggagcac gccgcccgcc tcgcgacgaa agccgagccc ggcatggtgc
     4741 ccccccaccc gggcgggggg ccacggtgat gcccgtgacc gccgtgatcg gcgcccagtg
     4801 gggagacgaa ggaaagggca aggtggtgga caccctggcc cgccgggccg acctggtgat
     4861 ccggtacaac gggggcaaca acgccggcca cacgattcag aaccagtacg gcacgttcag
     4921 gctccacctg ctgccgtccg gcatcttcca cccggccgcc cagtgcatca tcggcccggg
     4981 cgtcgtcatc aatcccgatg tcctcctggc cgaggtcgcc gacgtggagg gggtcgggat
     5041 gccgacggtt ggccgcctgt ggctctccga ccgcgcccac ctgatcttcc cgcaccacat
     5101 cctcatcgat gagctcgaag aggccgtgcg gggtggaagt ccccatggga cgaccaagca
     5161 ggggatctgg cccgtctaca gcgacaaggt cggacgggtc ggcatccggc tcggggatct
     5221 tcttgaggag cagcaccttg tcggccagct gacctatctc gcggaccgaa agagcgcggt
     5281 gctgcggggc gtgtaccggc acgaaccggt cgaggccggg caactgctcg ccgcatgcca
     5341 gcggtgggcg cagcgcctca ggccctatat caccgacacc caccccctga tccaagaagc
     5401 cctgcgggcc gaccgggtgg tgctgctcga gggtcagctg ggggtcatgc gcgacctcga
     5461 ctggggcctc tatccgttcg tgacctcgtc cacgacccta ccgggaggcg cgagcgccgg
     5521 agccggaatc cccccgtacc ggatcacccg cgtgctcggc gtggccaagg cctataccac
     5581 cgcggtcgga tcgggcccca tgccgaccga gctcgccgat gcgacgggcg agcgcatccg
     5641 ggacctcggg caggagtttg gcgccacgac ccgcaggccg cggcggtgcg gatggtttga
     5701 cggggtggcg gcccggtttg cggcggaggt cgcagggttt acgcacctgg cggtgatgaa
     5761 ggtcgacgtc ttcgactcct tcgagcagat ccgtgttgcg gtcgcttatc gtctggatcg
     5821 gcgaacggtg cacaccgtcc cgcataccgc ggcgatggcc cgggtcgagc ccatctacga
     5881 ggaacttccg ggatggcggt gctcgacgca cggagcgcgg cgtctcgggg acctccccat
     5941 cgaggcgcgg acgtttctcc ggcggatcga ggagatcgtc ggggtctcga tcgcgctggt
     6001 gggggtgggc ccggaccggg aggacatgat ctactgccag gagggctggg cgtgatcccc
     6061 cgctacactt cgccggagat gggggcgctg tggagccccg agacgaagtt cgccacctgg
     6121 ctcgagatcg agctcctcgc cgccgaggcg caatcgcgcc tggggaggat tccggaagcg
     6181 gcggtgcggc ggcttcggga gcgggcccgg gtggggagcg tcgcccggat cgacgagatc
     6241 gaggagcggg agacgcggca cgatgtcgtg gcgttcctgc gggttgtcgg cgagacggtg
     6301 ggcgacgacg cccgctacct ccacctgggg ctggggtcct cggatgtcgt ggacaccgcc
     6361 ctgtcggtcc tcatggtgcg cgcggccgac ctgatcggcg cctcgctcgg gcgcctgcac
     6421 cgggccctcg cggccctggc ccagaagcac cggtacacga tcatggcggg gcggacccac
     6481 ggggtccatg ccgagccggt gacgttcggt ttgaaggcgg ccctctggta tgccgaggtg
     6541 ggccgcgggt tggaccgaat ccggcgggcc cgcgaggtga tcggcgtggg caagatctcc
     6601 ggggaggtgg gcacgttcgc ccacacgccc cccgaggtgg aggcgttcgt ctgcgagcgg
     6661 ctcggcctcc agccggcccc ggcgtcctcc caggtgatcc agcgcgaccg ccacgccgag
     6721 tacctggcgc acctcgcggt cgtcgccggc acgctcgaga agatcgcgac cgagatccgc
     6781 cagctcgcgc gcaccgagct ccgcgaggtc gaagagccct tccgggaggg acagacgggg
     6841 tcctccgcga tgccccacaa gcggaacccg gtcctctgcg agcgcgtgac cggattgtcg
     6901 cgcgtggtcc gcgggcatgc ggtggcggcg ctcgagaaca tcgcgctctg gggggagcgc
     6961 gacatcaccc actcgtcggc tgagcgcgtc atcatccccg acgcgacgtc cctgctcgac
     7021 tacatggcgc gcacgctcgc gggcgtgatc gaaggcctgc gggtctaccc cgaccacatg
     7081 cggcaaaacc tcgatcggag cggggggctc gtgttctccc accgggtcct gctggccctc
     7141 ctcgatcgcg ggcggtcgcg ggaagaggcg taccggatcg tgcagtccgc cgcgatgcgg
     7201 gcgtgggaag agcagggcgg gttcagggag ttgctgcgcg cgtccggggg gctctccgac
     7261 gccgagctcg acgtctgctt cgatccctcc tacgcgctgc ggcacgtgga cgagatcttt
     7321 gcccgcgtcg ggctcgggga gcggcccgcg gtgccggcgg gcggccaggc ggaccgcgag
     7381 tcgaaagccc gcaggggggg aagcgccgat gcatgacgtg gtggcggatg ggcaggccgg
     7441 ggtggtgctc gagacccacc tcgatctccc gctcttctcg cgcggcaagg tccgcgatat
     7501 ctacgacctg ggggaccggc tgctggtcgt cgccaccgat cggttgtcgg cgttcgacgt
     7561 cgtgctgccg accgggatcc ccgacaaggg gcgggtgctg acggggctct cggccttctg
     7621 gtttgaacgg cttgcgcgga gggtgccgac ccacttcctc accgccgacg tgaacgcgtt
     7681 tcccccacac ctccgcgagc ctcgggcggt gctcgagggt cgatcgatga tcgtccgcaa
     7741 acttcgtcgg atcgatatcg agtgcgtggt ccgcggctac ctggccgggt cggcgtggaa
     7801 ggagtacgcg gccaccggcg gcgtgagcgg gcttgtgctg ccccgggggc agcgtccggg
     7861 ggcccggctg cccgcccccg tgttcacgcc cgcgacgaag gccgcatcgg gccacgatga
     7921 aaacatcagc gagggccgga tggccgagat cgtcggcggc gccctgacgc gccggctgcg
     7981 cgaggcgagc ctggcgatct accgcgaggc ggcccagacc gcggagcggt gcgggctgct
     8041 gctcgcggat accaagttcg agttcgggct cgacggcgac gagatcgtgc tgatcgacga
     8101 ggtgctgacc ccggattcgt cgcgcttctg ggacgccgcc gcctacgccg ccacggggtc
     8161 aaccgagagc tacgacaagc aggtcgtgcg cgactacctt gaacgggtcg ggtggaacaa
     8221 gcggcccccg gcccccgcgc tcccccccga ggtggtcggg gcgacgcgcg cgcggtatct
     8281 cgaggcctac cgccggctga ccgggcggga gctttcggcc tgagggagac ggtgatgccg
     8341 accgaacaag gcgcggagca ggtacgaacg aggcaggcgc ggaccgtgcg gatcgtggtg
     8401 acgctcaagc ccggcgtgct cgatgcgccc ggccaggcga tccgccaggg cctggacgcg
     8461 ctcggccatc ggggcgtcca cagcatccgg gcgggcaagt acttcgaggt ggacctcgcc
     8521 gacgacggcc ggatcgagga gcgggtccgc gagatgtgcg aagggttcct cgccaacacg
     8581 ctgatcgaag aataccgcta cgaggtcctc tgagggaggg cggccgtgga cgtcgggatc
     8641 atcgtgttcc cgggcagcaa ctgcgacgcg gacacctacc atgtcgtccg cgacgtcctg
     8701 ggctatcggg cgcggtacgt ctggcacgag gagcggcaac tcgccggcct cgacgcggtg
     8761 cttctccccg gcgggttcgc gtacggcgat taccttcggg ccggggcgat cgcggcgacc
     8821 tcaccggtgg tccgcgcgct gcggggatat gtggaggccg gggggctcgt cctcgggatc
     8881 tgcaacggct ttcaggtgct cctcgaggcc ggactcctcc ccggcgctat gcgcctcaac
     8941 gcgagcgggc agtttcggtg cgagttcatc cacatccggg ccgagcgggt cgacaccccc
     9001 tttacgtgcg cgatgacccc ggggcaggtg ctccggctcc cgatcgccca cgcggaaggg
     9061 aactactacg tcgacgcccc gacccaccag gcgctcgtgg cgggccgcca gatcgtgttc
     9121 cggtactgcg atccgggggg gaccgtcacc ccgcaggcga accccaacgg ctcgtacgac
     9181 gcgatcgcag ggggggcgag cccgggcggg accgtggtcg ggctgatgcc gcaccccgag
     9241 cgcgcaagcg aggatctcct ggggagcgcg gacggccgcc tggtgttcga gtcactgcac
     9301 cggtgggtca cggcgggggg gcgcccggcg ccagaagcgg tcgcggcggc caccggcgcg
     9361 gaggccgggt gaggcgatgc aaaccgtacc ggtaaccgac ctcgccggga ccgggctccg
     9421 gcccgcagag tacgacgaga tctgccgccg tctcggccgc gctcccaacc gggtcgagct
     9481 ggcgatgttc ggcgtgatgt ggagcgagca ctgcgcatac aagcactcga agggggtgct
     9541 ccggcggctc ccggtccgag gccgccgcgt gctcgcgggg ccgggggaga acgccggtgc
     9601 ggtgatggtc gccgatgggt gggcggcggt ctttaagatg gagagccaca accacccgag
     9661 cgccgtggcc ccgttccatg gagcggccac gggcgtgggc gggatcattc gcgacatcct
     9721 ggcgatgggc gcccgcccca tcgccctcct cgactccctc cgattcggcc cccccgagga
     9781 ccctggggtc gccccgttga tgcgcggtgt ggtggccggc atcgccgcct acgggaacag
     9841 catcggggtg cccacggtcg gcggagagct catccacgcg ccgtgctatc gggcaaaccc
     9901 cctggtgaac gtggcctgcc tggggatcgt ccgcgcggat cgcctcgcga ccgcccgtgc
     9961 gtccggccc
//
LOCUS       DASQGH010000179         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31995, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000179 DASQGH010000000
VERSION     DASQGH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31995"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VEW91_03415"
     CDS             complement(<1..874)
                     /locus_tag="VEW91_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYM90669.1"
                     /translation="MGPISQHAFGGIPQILQALEQQGYIAGRDVALSIHLSVVLRKPL
                     LIEGAAGVGKTEVAKVMARALETELIRLQCYEGLDVHTALYEWNYQRQMLRIKLEETS
                     GRPVEEKEHLIFSEPFMLKRPLLQAITADRSPVLLIDEVDRADEEFEAFLLEVLSDFQ
                     VSIPEIGTIQARHIPHAVLTSNRTRDLSDALRRRCLYLWIDYPSFEKELAIIHRKVPG
                     INERLAQQIGAFMQMVRRVDLDKVPGIAETLDWSAALLALHRDHLDPDAVDETLGCLC
                     KVHEDLTRVRAQYLR"
     gene            complement(945..1289)
                     /locus_tag="VEW91_03420"
     CDS             complement(945..1289)
                     /locus_tag="VEW91_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HYM90670.1"
                     /translation="MPRGGRAVQAERARRHFAYLLRCADGTYYAGYTVDPLRRLVAHR
                     LGQASRYTRGRGPLSLAAVWHCPTRRAALRLERMLKRLPHAAKQRLASGDPIGHVVAG
                     AARLGIRRRMMC"
     gene            1416..3749
                     /gene="cutA"
                     /locus_tag="VEW91_03425"
     CDS             1416..3749
                     /gene="cutA"
                     /locus_tag="VEW91_03425"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HYM90671.1"
                     /translation="MSTRYAGARIPRNEDPALLRGDGLFVDDLRLPGMVHAAVLRSPH
                     AHARIERIDVRRARAAPGVLDVLTHADLGALGAPLPKLIPHPALTHHKTQQALASSVV
                     RHVGEPVAFVVAESRYAAEDAAELIDVAYAPLPAAVALEAAAAPGAPLVHEDMASNIC
                     ARYTQRVGDVARALAQAPHRFRERLVLDRGTAASMETRGVVASWNPRTAHLEIWDSTQ
                     APIPIRNGLAAMFHLPQGNVRVVAPDVGGGFGPKVMMFYPEEILVPLASQRLGRPVKW
                     IEDRRENFVATNQEREQIHDAEIAVDSSGRILAVRTVFLYDAGAYCPYGLIVPIVAST
                     TLPGPYRIPHYHGEFTAVFTNKTTVSPYRGAGRPHGVFVMERLLDRAARELGVDRAEI
                     RRRNLIQPDEFPYEVGLVYQDNAPVRYDSGNYPQCLRRAVDAIGYPGWAARQAAQRAA
                     GRYVGLGIACYVEGTGIGPYEGCRVTVEPSGKVFVATGVGTQGQGHMTALAQLVADAL
                     GVTPRDVIVRTGDTAEFGWGTGTFASRAAVVAGTAAALAARAVGEKTRVVAAMLLEAR
                     PDEIELDAGKAFVRGVPARAIPLGEVARAANPLRFVMPAEWEGPGLEATRYFAPPQAA
                     FSNGVHSCIVEVDPETGALAILRYVVVHDCGRVLNPMILEGQVHGGVAQGLGGAFWEK
                     LAYDAQGQPLATTFMDYLLPTAAEVPPFEVVHEETPSPLNPLGVKGAGEAGVIPVAAV
                     IAQAIEDALRPFGVHITQMPLSPDRLQHLVTEARRSP"
     gene            3746..4189
                     /locus_tag="VEW91_03430"
     CDS             3746..4189
                     /locus_tag="VEW91_03430"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HYM90672.1"
                     /translation="MRVEGTNTLPAPVETVWKKIIDPEVLRRCTPGLQELKETAPDTY
                     RATLVIGIAAVKGTYAGTLALTDKRPPTHCKIALEGTGGAGFMKGEGTVDLEPQGDGT
                     LARWVADIQIGGLIAGVGQRMLGGIGKMLIGQFFKSLEEELGGRT"
     gene            4186..5058
                     /locus_tag="VEW91_03435"
     CDS             4186..5058
                     /locus_tag="VEW91_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYM90673.1"
                     /translation="MKPAQFDYLCPRTVDEAVDLLARHGDDAKVLAGGQSLVPLMNMR
                     LARPAVIIDINRIAALEYLREEHGVLRLGALTRQRAAERSPLVADRCPLLRDALRLVG
                     HVQIRNRGTIGGSIAHADPAAELTAILVALDGEITVRSARGTRVIPAADLFVTYLTTS
                     LDPRELLVEVRIPALPRGAGWSWMEIARRHGDFALAGVGVVLTVRRGAIAEARIALTG
                     VGPTPVRAARAERLLVGRGPSEALWGEAAEAVRAAVTPEEDIHASAEYRKHVSGVLAQ
                     RALREAAARAGEAA"
     gene            5058..>5296
                     /locus_tag="VEW91_03440"
     CDS             5058..>5296
                     /locus_tag="VEW91_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYM90674.1"
                     /translation="MALHQITLRVNGRAYSGQAEPRRTLADFIREDCGLTGTHLGCEH
                     GVCGACTILMDGEAVRSCLLFAVQAEGAELQTVEGL"
BASE COUNT          791 a         1850 c         1889 g          766 t
ORIGIN      
        1 gccgcaggta ctgcgcgcga acgcgggtga gatcctcatg aaccttacac agacacccca
       61 gggtctcgtc gaccgcgtcg ggatcgaggt gatcgcggtg gagcgccagc agtgccgcgc
      121 tccagtccag ggtttccgcg atgcccggga ccttgtcgag atcgacgcgg cgcaccatct
      181 gcatgaaggc gccgatctgc tgcgccagcc gctcgttgat ccccggcacc ttccggtgga
      241 tgatcgccag ctctttttca aaggacgggt agtcgatcca caggtacaga caccggcggc
      301 gcagggcgtc gctgaggtcc cgggtccggt tgctcgtcag gaccgcgtgc gggatgtggc
      361 gcgcctggat cgtgccgatc tccgggatgc tcacctggaa gtcggagagc acctccagga
      421 ggaacgcctc gaactcctca tcggcccggt ccacctcgtc gatgagcagc accggcgaac
      481 ggtcggccgt aatggcctgc agcagcggtc gcttcagcat gaacggttca ctgaagatca
      541 ggtgctcctt ctcctccacc ggccggcccg aggtctcctc gagtttgatc cgcagcatct
      601 gtcgctggta gttccattcg tacaacgcgg tgtgcacgtc cagcccttcg tagcattgaa
      661 ggcggatcaa ttcggtctcg agggcgcggg ccatgacctt ggcgacctcg gtcttgccga
      721 cgccggccgc gccctcgatc agcagcggct tgcgcagcac gacgctcagg tgaatcgaca
      781 gcgccacgtc gcggccggcg atgtacccct gctgctccaa ggcctgcaga atctggggga
      841 tccccccgaa cgcatgctgc gagatcggtc ccatctgcgg caatggctcc tcctgctggg
      901 tcctgcggtg ttcaccgcaa gacgcgcctt ccgacccatc cattttagca catcatccgg
      961 cgcctgatcc cgagccgggc ggcaccggcc accacatgcc cgatcgggtc gcccgacgcc
     1021 agccgctgct tggcggcgtg cggcaggcgc ttcagcatgc gctccaggcg cagggccgcg
     1081 cggcgcgtgg ggcagtgcca gacggccgcg agggagagcg ggccgcggcc gcgcgtgtag
     1141 cgcgacgcct gtccgagtcg atgcgccacg aggcgccgca ggggatccac ggtgtagccg
     1201 gcgtagtagg tgccgtcggc gcaccgcaac aggtacgcga agtgccggcg ggcccgctcc
     1261 gcctggaccg cccgcccccc tctgggcatc ggtgagaagg attcgccccg cgcccaggta
     1321 cctccctgga ccccaccgat gcgcgaaagg atgcgcgaaa ggatgcgcga aaggatagga
     1381 gatcccgcgc cgagcaccga aggaagatct cccctatgag cacccgctac gccggcgcac
     1441 gcatcccccg caacgaggat cctgcgctgt tgcgcgggga cggattgttt gttgacgacc
     1501 tccggctgcc gggaatggtc cacgccgcgg tgttgcgcag cccacacgcg cacgcccgca
     1561 tcgaacggat cgacgtgcgc cgtgcgcgcg ccgcgcccgg cgtcctcgac gtcctcaccc
     1621 acgccgatct cggcgccctc ggcgctcccc tgcccaaact cattccgcat ccggcgctca
     1681 cccaccacaa gacccagcag gcgctcgcct cgtcggtcgt ccggcacgtg ggggagccgg
     1741 tcgcgtttgt cgtcgcggag agccgctacg cggcggagga cgccgccgag ctcatcgacg
     1801 tggcctacgc gccgttgccg gcggcggtgg ccctcgaagc ggcggccgcg cccggcgcgc
     1861 cgctggtgca cgaggacatg gccagcaaca tctgcgcgcg ctacacgcag cgggtcggag
     1921 acgtcgcgcg cgcgctcgcc caggccccgc atcggttccg ggagcggctc gtgctggatc
     1981 gcgggacggc ggcgtcgatg gagacccgag gcgtcgtcgc atcgtggaat ccgcggaccg
     2041 cgcacctcga gatctgggac agcacccaag cccccatccc gatccgcaac ggcctcgcgg
     2101 cgatgtttca cctgccccaa ggcaacgtcc gcgtcgtggc gcccgacgtc gggggcgggt
     2161 tcgggccgaa ggtcatgatg ttctaccccg aggagatcct ggtcccgctc gcctcgcagc
     2221 ggttggggcg gccggtcaag tggatcgagg accgccggga gaattttgtg gcgacgaacc
     2281 aggagcggga gcagatccac gacgccgaga tcgccgtcga ctcgagcggc cgcatcctgg
     2341 ccgttcggac cgtgttcctg tacgatgcgg gcgcctactg tccgtacggc ctcatcgtgc
     2401 cgatcgtcgc cagcaccacg ctccccggcc cgtaccggat tccgcactac cacggcgagt
     2461 tcaccgccgt cttcacgaac aagacgacgg tcagcccgta tcggggcgcg ggccggcccc
     2521 acggggtgtt cgtgatggaa cggctcctcg accgggcggc ccgcgagctc ggcgtggacc
     2581 gggccgagat ccgccggcgc aacctcattc aacccgacga gtttccgtac gaggtggggc
     2641 tagtctatca ggacaacgcg ccggtccggt acgacagcgg caactatccg cagtgcctcc
     2701 ggcgcgcggt ggacgcgatc gggtatccgg gctgggcggc gcgtcaggct gcgcagcgag
     2761 cggcgggccg gtacgtcggg ctcggcatcg cctgctatgt ggaagggacc ggcatcgggc
     2821 cgtacgaggg gtgccgggtc acggtggagc cgtcggggaa ggtcttcgtc gccaccgggg
     2881 tgggcacgca gggccagggc cacatgacgg cgctcgccca gctcgtggcc gacgcgctgg
     2941 gggtgacacc ccgggacgtc atcgtgcgca ccggggacac cgcggagttc gggtggggca
     3001 ccgggacgtt cgcgagccgc gcggcggtcg tggccggcac ggcggcggcg ctcgcggccc
     3061 gggccgtggg cgagaagacg cgcgtcgtgg cggcgatgct cctggaggcc cgaccggatg
     3121 agatcgaact cgacgcgggc aaggccttcg tgcggggcgt ccccgcgcgc gcgatcccgt
     3181 tgggggaggt cgcgcgcgcg gccaacccgc tgcggttcgt gatgccggcg gagtgggagg
     3241 gcccggggtt ggaggccacg cggtattttg ccccgccgca ggccgccttc agcaacggcg
     3301 tccactcctg catcgtggag gtcgacccgg agaccggagc gctggcgatt ctccgctacg
     3361 tcgtcgtcca tgattgcggc cgcgtgctta acccgatgat tctggaagga caagtccacg
     3421 gcggggtggc gcaggggctc ggcggggcgt tctgggagaa gctggcgtac gacgcgcagg
     3481 ggcagcccct cgcgaccacc ttcatggact acctgctccc cacggccgcc gaagtccccc
     3541 cgttcgaggt tgtccacgaa gagacgccgt cgccgctcaa ccccctcggc gtcaaggggg
     3601 cgggggaggc gggggtgatc cccgtcgcgg ccgtcatcgc gcaggcgatc gaggacgcgt
     3661 tgcgaccgtt cggcgtgcac atcacccaga tgccgctcag cccagaccgc ctgcagcacc
     3721 tcgtgactga agcgcggagg tccccatgag agtagaaggc accaacacgc tccccgcccc
     3781 ggtggagacc gtctggaaga aaatcatcga tcccgaggtg ctgcgccggt gcaccccggg
     3841 cctccaggag ctcaaggaga ccgcccccga cacctatcga gccacgctcg tgatcgggat
     3901 cgcggcggtc aagggcacct acgccggcac cctggcgctc acggacaagc ggccccccac
     3961 ccattgcaag attgcgctcg agggaaccgg aggcgcgggg ttcatgaagg gcgaggggac
     4021 cgtcgatctc gaaccgcagg gagacggaac cctggcccgg tgggtcgccg acatccagat
     4081 cggtgggttg atcgccggcg tggggcaacg gatgctcggc ggcatcggga agatgctgat
     4141 cggacagttc ttcaaatccc tggaggagga actcggaggg cgtacgtgaa gccggcgcag
     4201 tttgactacc tctgcccccg gacggtcgac gaagcggtcg atctgctggc ccggcacgga
     4261 gacgacgcga aggtgctggc cgggggccag agcctcgtgc cgttgatgaa tatgcgcctg
     4321 gcccgcccgg cggtgatcat cgacatcaac cggatcgccg ccctcgagta ccttcgggag
     4381 gagcacggcg tgctccgcct gggggccctc acccgccagc gtgcggcgga gcgctcgccg
     4441 ctcgtggccg accggtgccc gctcttgcgc gatgccctgc gcctggtcgg ccacgtacag
     4501 atccgcaacc gcggcacgat cggcggcagc atcgcccacg ccgacccggc cgcggagctg
     4561 accgcgatcc ttgtcgcgct cgacggcgag atcaccgtcc ggagcgcccg cggcacccgg
     4621 gtcatcccgg cggcggacct gttcgtgacg tacctcacga cctcgctcga tccgcgagag
     4681 ctgctggtcg aggtgcggat ccccgccctg ccccggggcg cggggtggtc ctggatggag
     4741 atcgcccgcc gccacggcga ctttgcgctg gccggcgtgg gcgtcgtgct caccgtccgc
     4801 cgcggggcga tcgccgaggc ccggatcgcc ctcacggggg tgggaccgac gccggtccgg
     4861 gccgcccggg ccgagcgcct gctcgtcggg cgggggccct cggaggcgct gtggggggag
     4921 gcggccgaag ccgtgcgggc cgcggtcacc ccggaggaag acatccacgc ctcggccgag
     4981 tatcgcaagc acgtctccgg ggtgctcgcc cagcgtgccc tcagggaggc ggcggcgcga
     5041 gcgggagagg cggcctgatg gcgctccatc agatcaccct ccgcgtcaat ggcagagcct
     5101 actccgggca ggcggaaccc cgcaggaccc tggccgattt catccgggag gactgcggcc
     5161 tgaccggcac gcacctcggg tgcgagcacg gcgtgtgcgg cgcctgcacg atcctcatgg
     5221 acggcgaagc ggtccgctcc tgcctcctct tcgcggtgca ggcggagggc gctgagttgc
     5281 agacggtcga agggct
//
LOCUS       DASQGH010000180         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14939, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000180 DASQGH010000000
VERSION     DASQGH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14939"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VEW91_03445"
     CDS             complement(<1..650)
                     /locus_tag="VEW91_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYM90675.1"
                     /translation="MPTEVVLPRLGLTQEEGTVVRWLKSEGSSVSKGEPLFEVMTDKA
                     TLEVEAPVSGVLLRILVAAGGTVPVATPIALIGESGETVPAVPGPQAIPATAAGTALL
                     GGRPPEMESVAPVVSARVPAPDEETPARGPAGERIKISPRARALAETHGIDVRTLVGT
                     GPGGRIIEHDVQMAITSRSTAPAAPPVSPPPPPATSFTPVPPPAAPPAAATPWAAAP"
     gene            complement(681..1085)
                     /locus_tag="VEW91_03450"
     CDS             complement(681..1085)
                     /locus_tag="VEW91_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYM90676.1"
                     /translation="MDVIGVSRGKSLPEPPRKDRIQPDALVGGHEMFTAFVMLQAERR
                     RLQEVARGLAEIRGIEEVYSVTGEWDIIAVLRLKDYNDLAEVVTGKMVEIPGIVRTNT
                     HLAFRAFPQSLLERSFSVGLEEEAEGRGTAEE"
     gene            1180..1530
                     /locus_tag="VEW91_03455"
     CDS             1180..1530
                     /locus_tag="VEW91_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HYM90677.1"
                     /translation="MMAKVYAQVVGIVLLLIGVVGLFTSSLLGAKTTVDHNLIHLVSG
                     AIGAYTGFSGSGFRQFAQIFGIVYTLVGVIGLITPSTLGGLGVPVNTTYNLIHLVIGL
                     WGLWAGFSKEMATA"
     gene            complement(1613..>2025)
                     /locus_tag="VEW91_03460"
     CDS             complement(1613..>2025)
                     /locus_tag="VEW91_03460"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HYM90678.1"
                     /translation="LAEALPALLARARRLRVPRMTLRHLIRARGTAAAAVLDCVEERP
                     AWGEPLCRGWPHIGAEVVVAVRDEMAVTLADVLLRRTRLALLLPGQGTEIAAQVAALM
                     GDELAWSQDARSAQVADYTRAASHFAVSGITTPV"
BASE COUNT          307 a          729 c          675 g          314 t
ORIGIN      
        1 ggcgcggccg cccagggggt agcggctgcg ggcggtgccg ccggcggtgg gacgggcgtg
       61 aaagacgttg caggaggagg tggcggcgaa accggcggcg cagccggcgc tgtggaacgg
      121 gaggtgatcg ccatctggac gtcgtgctcg ataatccggc cgcccgggcc ggtgcccacg
      181 agcgtgcgta cgtcgatacc gtgcgtctcc gcgagcgcgc gggcgcgggg agagatcttg
      241 atccgctcgc ctgccgggcc cctcgcgggc gtctcctcgt cgggcgcagg gacacgggcc
      301 gagaccaccg gggcgaccga ttccatctcc gggggccgtc cacccaggag agctgtgcct
      361 gccgccgtgg ccgggatagc ctggggaccc gggaccgcgg gaacggtttc acccgactcg
      421 ccgatgagcg cgatcggggt cgcgaccggg acggtacctc ccgcggcgac gagaatccgg
      481 agcagcaccc cggagaccgg cgcctcgacc tcgagggtgg ccttgtcggt catgacctcg
      541 aagaggggct cacccttgct gacggagctc ccctccgatt tgagccaccg gacaacggtc
      601 ccctcttcct gcgtcagccc cagcctcggg aggacgacct cggtcggcat caagccacca
      661 gccggcggat ggcgttcacg ctactcctcc gccgtccccc ggccctccgc ctcctcctcg
      721 agccccacgg agaacgaccg ctcgagcagc gactggggga aggcgcgaaa tgccaggtgg
      781 gtatttgtcc ggacgatccc cggaatctca accatcttgc cggtcacgac ctcggcgagg
      841 tcgttgtagt ccttgagccg cagcaccgcg atgatatccc actctccggt gaccgagtag
      901 acctcctcga tcccgcggat ctcggcaagc ccgcgcgcaa cctcctgcag ccgccgccgc
      961 tcagcctgca acatcacaaa cgccgtaaac atctcgtggc ctccgaccag agcgtccggt
     1021 tggattctat cttttcgcgg cggctcgggc aaactcttgc cccggctcac cccgatcacg
     1081 tccatcggag ccccaaccgt cgcgtcgctt gacgaggccg gggtttgtga taggctgagg
     1141 ctggacgttg ccgcactcat ccacacagag gggggatgca tgatggccaa ggtctatgcg
     1201 caggtcgttg gaatcgtatt gctcctcatt ggagtcgtcg gcctgttcac gtcctcgttg
     1261 ctcggcgcga agaccaccgt ggaccacaac ctgatccacc tcgtcagcgg tgcgatcggg
     1321 gcctatacgg ggttctccgg ctccggcttc aggcagttcg cccagatctt cgggatcgtc
     1381 tacacgctcg tgggggtcat cggtctcatc accccgtcaa cgctcggcgg cctcggggtc
     1441 ccggtgaaca ccacgtacaa cctcatccac ctcgtgatcg gcctgtgggg gctgtgggcc
     1501 ggcttcagca aggagatggc gaccgcctga tcgacagagc aacgtatcca cagggccccg
     1561 gtggaagccg gggcccttgt cattccgcgg ttccttgcgc gcggcaccgt cctcacaccg
     1621 gggtcgtgat ccccgacacc gcgaaatgcg acgcggcccg ggtgtagtct gcgacctgcg
     1681 ccgatcgtgc gtcctgcgac cacgcgagct catcccccat cagcgccgcc acctgcgcgg
     1741 cgatttccgt cccctgaccg gggagcagaa gcgcgagccg ggtccggcgc aggagcacgt
     1801 ccgccagggt gaccgccatc tcgtcgcgta ccgccaccac cacctcggcc ccgatgtgcg
     1861 gccatccccg gcacagcggc tccccccatg cgggccgctc ctcgacgcag tcgaggacgg
     1921 ccgcagccgc cgtgccccgg gcccggatca gatggcgcag ggtcatccgg gggactcgga
     1981 gccgccgggc gcgggccagc agggcgggca gcgcctcggc cagcc
//
LOCUS       DASQGH010000181         8684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34714, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000181 DASQGH010000000
VERSION     DASQGH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34714"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1649
                     /locus_tag="VEW91_03465"
     CDS             120..1649
                     /locus_tag="VEW91_03465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYM90679.1"
                     /translation="MPGPLTWLILLPVLAAAGAAAVDRRHERSVRTLGIVAAAGVFVL
                     AAWVWGRFAPGATGLQFEERVPWIPKAGIVYHLGVDGLSVTLVLLTAFLFPLALAAAA
                     TQVRARVKAFVVTALLLEAGMLGMFVAQDLVLFYVFWEGMLVPMYFLIGLWGGPGRAR
                     AAIKFFLYTMAGSVLMLLAIVAVYLQSERVLGEATFDLPTLLTHPLGIGGRLGTVLFL
                     AFAVAFAIKMPVWPLHTWLPDAYAEAPPIVTVLLASLMAKAGAYGLLRFCLPLFPDAM
                     RAFGPAASLLAVVGILYGGAVAWAQADLRRLLAYGSMSHMGFILLGIFALNVTAVQGS
                     LIQMINHGVSTGALFFIAGMLIERTGGARTDEYGGLAAVTPALAAATLIVVLSSLALP
                     GTNGFIGEFLILLGTFQVRPVAAALATFGVVVTAAYLLAFVGRVFHGPARPALASMAD
                     LRRWEVAVLAPLIAAIFWVGLYPHPLLARSEAAVQALLARVPASVPWHDDAALRAGPP
                     R"
     gene            1646..3085
                     /locus_tag="VEW91_03470"
     CDS             1646..3085
                     /locus_tag="VEW91_03470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYM90680.1"
                     /translation="MTASRALALIGPEAMLVLGAFLVLLWDAWETGAALESDAPRRAM
                     PWLALATVLAALGWTVAGGSPGSGFAGMYVRDPLTRMIDLAALGAAGIAVLLSPAYLT
                     RVRLPRGEYYALVLLSTVGAMVMAASGTLLTLFLGLEILSLPLYILAGLARRSRRSQE
                     AGLKYLLLGAFATAFFAYGVALVYGATGSIALGRIAAAPRSSMLGAGVALLTIGLGFE
                     AALVPFHAWAPDVYEGAPLPVTAFMSVVAKIGAFAGLLRVFPLALPQFAAQWGQALAA
                     IAILTMILGNLAALLQTNLKRLLAYSGIAHAGYLLMGVASGGPAGIWSVLFYLLVYTG
                     MTLGAFGILLLLERQGAEADRLEDLAGLAARAPWAAVALVLFMVSLAGLPPTAGFIAK
                     LYLFRAALGAHQTALALVGVLTSVVAVYYYLRVAYVALTGEASQAVAVFRNGFVGAAL
                     AVAAVGVLWLGLFPAGFTAAVQQAAQVIR"
     gene            3097..4398
                     /locus_tag="VEW91_03475"
     CDS             3097..4398
                     /locus_tag="VEW91_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90681.1"
                     /translation="MPSAEEFDAIVVGAGPGGVSAALTMARAGLSVVLFERGERPGVK
                     NVMGGVMYGRMLADVIPEFWKTAPIERIVVDERLWLTTADAAVTAGHKHVPHTDGSPN
                     AFTVLRVPFDGWFAEQAEAAGVLLIPGTTVEDVLREGGRIVGVRTGREEGDLRANLVV
                     IADGANSFLVQKAGLGPPLGPNEMALVVKEIIALSPEVIEERFTLDPGMGATIEVYGA
                     VTRGMAGYGFIYTNRGSLSVGIGALISQFMATRITPWDLLEGFKAHPMVAPLLRGGAV
                     REYLAHAIPEGGFRAMPRLFDDGVMVVGDAAMMVNNLHREGSNLAMAAGRMAGETAIQ
                     AKRVGDWSARTLAQYRELLEASFVLQDLRKYARLPELVERRPDLLQLYPELLSEAVHE
                     MLTVDGASKREKQRRILRRLLGRRPWWRLLRDAYEGWRAMR"
     gene            4395..4679
                     /locus_tag="VEW91_03480"
     CDS             4395..4679
                     /locus_tag="VEW91_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYM90682.1"
                     /translation="MKIEEKLFLLRWKHDKRSHISITNPRVCLEACGEAWKRPCTTFC
                     PASVYAWDGEKIAVSHDNCVECTSCVLGCPYRNIDWKLPRGGFGIEWQHG"
     gene            complement(4698..6494)
                     /gene="polX"
                     /locus_tag="VEW91_03485"
     CDS             complement(4698..6494)
                     /gene="polX"
                     /locus_tag="VEW91_03485"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HYM90683.1"
                     /translation="MHRGPPGSVGEKNRVTNKAIARIFYDIADILEIRGENLFRAMAY
                     RNAGRSIELSAEDAGVLYRYGGRRALETIPGVGPTIASMIEQLVTTGHLKYYNRIARK
                     IPPVARALTRIPNIGPKTAMELYRALAVSSIEDLERVIDTPIAAVQVREKIRQRIKAG
                     IALLRRRSGQIPLPVAEPIARDLVEALRECPGVVSADPVGSLRRMRETVGDIDIVCAT
                     RDPEQAIGRFVSHPRAKQVLGAGVTKAAIIHADGVRVDLEIVPAEQYGSRLQHFTGSE
                     EHNARLRTWAMERGFSISEYGIEKDDRLHTFAHEEDVYKFLGMDWIPPELRENQGEIE
                     AALRHGLPDLVEERDIRGDLQSHSTWSDGHDQIDAVVRAAIDRGYEYLAITDRTKGVG
                     ITRGLDAARVAERHNEIAAAKRRYEGRIQLLEGVEVDIRADGHLALPDEILRTMDIVV
                     ASIHSAVAETTQRITRRLVAAIDHPYVDVIGHPSGRLLGEREAYPAGWDEVFAAAARR
                     RVALEINASPNRLDLNDTLAREAGRRGVDLAINTAAHKVEHLAMMRYGVAVARRAWLT
                     PAHVVNTRPWTRFSTWLYERKAFAREIRLTAA"
     gene            complement(6632..7081)
                     /locus_tag="VEW91_03490"
     CDS             complement(6632..7081)
                     /locus_tag="VEW91_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYM90684.1"
                     /translation="MSGAPHPAWDDVNRALAAAHNYWVATAGAGGRPHAVPVWGVWLD
                     GTFYFATARGSRKGRNLAENPRVVVHLESGDDVVILEGDADEVSSASMLARFADAYEA
                     KYRWRPGTDGDPGVTYALRPRVAFTWRERDFPQSAIRWLFTRSAGRP"
     gene            complement(7156..7962)
                     /locus_tag="VEW91_03495"
     CDS             complement(7156..7962)
                     /locus_tag="VEW91_03495"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYM90685.1"
                     /translation="MHPVLLRLGPLKLGHLVLGPVLLRWYGAMMGLALILSIPVTAHF
                     GGLFGIERRLLVDSLALPFLLSLLVGARAGFVLSHPQDFVGDPLAIIRPPYAGLASHG
                     AIAVALVYLAVWCPRHRVPLWRLADAMAPAFLLAIMLVRWGNFMNGELYGDPTALPWG
                     TVFPGVPGGPRHPLQLYEIAGTAVILGWALWAARRRAFDGALVWPVIIASSALRFALD
                     LLRSEDRTVVFLSWGQMAALALIVWGAWFLWRHRRGVRAGPGPHEGSGGV"
     regulatory      8041..8151
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            8239..>8684
                     /locus_tag="VEW91_03500"
     CDS             8239..>8684
                     /locus_tag="VEW91_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYM90686.1"
                     /translation="MAANGLVCRECGQRFPAEAIFVCESCFGPLEVEYDLGGLGGGAL
                     RERIAGGPASIWRYQDLLPVPRVPEWDLSPGFTPLVRAHRLGEALGLRHLYVKNDTRN
                     PTWSFKDRVVAVGLAAGKQFGFAVFSCASTGNLANAVAAHAAKAGLR"
BASE COUNT         1156 a         2970 c         3120 g         1438 t
ORIGIN      
        1 cgctgggtgg gccgcgccgg ccgggccctc tccggtctcc agaccggcta cctccggcac
       61 tacgcggcat tcgtcctgat cggggccctc ctcatcctcg tcttctgggt gtggcgctga
      121 tgcccggccc cctcacctgg ctgatcctgc tcccggtgct ggccgccgcg ggcgcggccg
      181 cggtggaccg gcgccacgaa cgcagcgtcc ggaccctggg gatcgtcgcc gcggcggggg
      241 tgttcgtcct ggctgcgtgg gtgtgggggc ggtttgcccc gggggcgacc ggcctccagt
      301 ttgaggagcg ggtcccctgg atcccgaagg cgggcatcgt gtaccatctc ggcgtcgacg
      361 gcctctcggt cacgctcgtc ctgctcaccg cgttcctctt tcccctcgcg ctcgccgccg
      421 ccgcgacgca ggtgcgggcg cgcgtcaagg cgttcgtggt gaccgccctg ctcctggagg
      481 cggggatgct cgggatgttc gtcgcccagg acctcgtcct gttctacgtg ttttgggagg
      541 ggatgctcgt cccgatgtat ttcctgatcg ggctgtgggg cggcccgggg cgggcgcggg
      601 cggcgatcaa gttcttcctg tacacgatgg ccggaagcgt cctgatgctc ctggccatcg
      661 tggcggtgta cctgcagagc gagcgggtcc tgggggaggc gacgttcgac ctcccgacac
      721 tcctcacgca tcccctcggg atcggcggac ggctcgggac cgtgctgttc ctggcgttcg
      781 cggtcgcgtt cgcgatcaag atgccggtgt ggccgctcca cacctggctg cccgatgcct
      841 acgccgaggc gccgccgatc gtgaccgtcc tgctggcgtc gttgatggcg aaggccgggg
      901 cgtacgggct gctgcggttc tgcctcccgc tgtttcccga cgcgatgcgg gcgttcggcc
      961 cggcggcctc cctgctcgcc gtcgtgggca tcctctacgg cggcgcggtc gcgtgggcgc
     1021 aagcggatct gcggcgcctc ctcgcgtacg ggagcatgag ccacatgggg ttcatcctgc
     1081 tggggatctt tgcgttgaac gtgaccgccg tgcagggcag cctgatccag atgatcaacc
     1141 acggggtcag caccggcgcc ctgttcttca tcgcgggcat gctcatcgag cgcaccgggg
     1201 gcgcccggac cgacgagtac gggggcctgg ccgcggtcac cccggccctc gcggcggcga
     1261 cgctgatcgt cgtgctgtcc tcgctggcgc tgccggggac caacgggttt atcggggagt
     1321 tcttgatcct gctcgggacg ttccaggtcc ggccggtcgc ggcggctctg gcgacgttcg
     1381 gggtggtcgt gacggcggcg tacctcctgg cgttcgtcgg ccgcgtcttc cacggtcccg
     1441 cgcgcccggc cctcgcgtcg atggcggatc tgcggaggtg ggaggtcgcc gtgctcgccc
     1501 cgctgatcgc ggcgatcttc tgggtggggc tgtatcccca tccgctgctc gcgcggagcg
     1561 aggcggcggt gcaggcgctc ctggcgcgcg tgcccgcgag cgtcccctgg cacgacgacg
     1621 ccgccctccg cgcggggccc ccccggtgac ggcgtcgcgg gcgctggcct tgatcggtcc
     1681 cgaggcgatg ctcgtgctcg gcgcgtttct ggtgctcttg tgggacgcgt gggaaaccgg
     1741 ggccgcgctg gaatcggacg cgccgagaag ggcgatgccc tggctcgcgc tcgcgaccgt
     1801 ccttgcggcc ctcgggtgga ccgtcgcggg gggaagcccg gggtccggat tcgccggcat
     1861 gtacgtccgc gatcccctga cccgcatgat cgacctggcg gccctgggcg ccgccggcat
     1921 cgccgtgctg ctctcgccgg cgtacctgac ccgggtgcgg ctcccgcggg gggagtacta
     1981 cgcgctggtc ctcctcagca cggtcggcgc gatggtgatg gcggccagcg ggacgctcct
     2041 caccctcttt ctcggcctgg agatcctgag cctaccacta tatatcctcg ccgggctggc
     2101 ccggaggagc cggcggtcgc aggaagccgg cctgaagtac ctcctcctcg gcgcgttcgc
     2161 caccgcgttc ttcgcctacg gggtggcgct cgtctatggc gcgaccggat cgatcgccct
     2221 cgggcggatc gccgccgccc cgcgctcgtc gatgctcggc gccggggtcg cgctcctcac
     2281 gatcgggctc gggttcgagg ccgccctcgt gccgttccac gcctgggcgc ccgacgtgta
     2341 cgagggggcg ccgctcccgg tgaccgcgtt catgtcggtg gtggcgaaga tcggcgcgtt
     2401 cgccgggctg ctccgggtgt tcccgctcgc gcttccgcag ttcgccgcgc agtggggcca
     2461 ggcgcttgcc gcgatcgcga tcctcaccat gatcctgggc aatctcgccg cgctgctcca
     2521 gacgaacctc aagcggctcc tcgcctactc cggcatcgcc cacgccggct acctgctgat
     2581 gggggtcgcg tccggaggac cggcggggat ctggagcgtg ctcttctacc tcctcgtcta
     2641 caccgggatg acccttgggg cgttcggcat cctgctgctg ctcgagcggc agggggcgga
     2701 ggcggaccgc ctggaggatc tcgcgggcct ggcggcgcgg gcgccgtggg cggctgtcgc
     2761 gctcgtgctg ttcatggtgt cgctcgccgg cctgccgccg accgcggggt tcatcgccaa
     2821 gctgtacctg ttccgcgcgg ccctgggagc ccaccagacc gcgctcgcgc tcgtgggcgt
     2881 cctcaccagc gtggtcgcgg tctactacta cctgcgggtc gcctacgtcg cgctgacggg
     2941 ggaggcgtcc caggccgtgg ccgtcttccg caacgggttc gtcggcgcgg cgctggcggt
     3001 cgccgccgtc ggggtgctgt ggctggggct gttcccggcg ggcttcaccg cggcggtgca
     3061 gcaggccgcg caggtgatca ggtagaatct cggcccgtgc cctccgcgga agagttcgac
     3121 gcgatcgtgg tcggagccgg ccccggcggc gtctcggcgg cgctcacgat ggcccgcgcc
     3181 ggcctcagcg tggtcctgtt cgagcgcggc gagcggccgg gcgtgaagaa cgtgatgggt
     3241 ggcgtcatgt acggccggat gctcgccgac gtcatccccg agttttggaa gaccgcgccg
     3301 atcgagcgga tcgttgtcga cgagcggctg tggctcacca ccgccgacgc cgcggtcacg
     3361 gccggccaca agcacgtgcc ccacaccgac gggtccccca atgcgttcac cgtgctgcgc
     3421 gtgcccttcg atgggtggtt tgccgagcag gcggaagccg cgggcgtcct cctcatcccc
     3481 ggcaccaccg tggaggacgt gctccgcgag ggggggcgga tcgtgggggt ccgcaccggg
     3541 cgcgaggagg gggatctccg cgccaacctc gtggtcatcg ccgacggcgc caactccttc
     3601 ctggtccaga aggccgggct ggggccgccg ctcgggccca acgagatggc gctggtggtc
     3661 aaggagatca tcgcgctgtc gccggaggtc atcgaggagc ggttcaccct cgacccgggg
     3721 atgggcgcga cgatcgaggt gtacggcgcc gtcacgcggg gcatggcggg gtacggcttc
     3781 atctacacca accgcgggtc gctgtcggtc gggatcgggg cgctcatctc gcaattcatg
     3841 gcgacccgga tcaccccgtg ggacctgctc gaagggttca aggcgcaccc gatggtggcg
     3901 ccgctgctgc ggggcggcgc ggtgcgggag tacctggcgc acgcgatccc ggagggcggg
     3961 ttccgggcga tgccgcgcct cttcgacgac ggggtcatgg tcgtgggcga tgcggcgatg
     4021 atggtgaaca acctccaccg cgaggggagc aacctggcca tggccgccgg ccggatggcg
     4081 ggggagaccg cgatccaggc gaagcgggtg ggggactggt cggcccggac gctcgcccag
     4141 taccgggagc tgcttgaggc ctcgttcgtc ctgcaagacc tccggaagta cgcgcgcctg
     4201 ccggagctcg tcgagcggcg gcccgatctc ctgcagctct atccggagct gctgagcgag
     4261 gcggtccacg agatgctgac cgtggacggg gcgagcaagc gggagaagca gcgccggatc
     4321 ctgcgccgcc tcctcggccg gcggccgtgg tggcggctgc tgcgcgatgc ctatgagggc
     4381 tggagggcga tgcggtgaag atcgaggaaa agctcttcct gctgcgctgg aagcacgaca
     4441 agcggtctca catcagcatc accaacccgc gcgtctgcct cgaggcatgc ggggaggcgt
     4501 ggaagcggcc gtgcacgacg ttctgtccgg ccagcgtcta cgcgtgggac ggggagaaga
     4561 tcgccgtctc gcacgacaac tgcgtggagt gcacgagctg cgtcctcggc tgcccgtacc
     4621 ggaacatcga ctggaagctc ccccggggcg ggttcgggat cgagtggcag cacgggtagc
     4681 cgggtcggtc tgacgaccta ggcggccgtg aggcggatct ccctcgcaaa cgccttgcgc
     4741 tcgtacaacc aggtggagaa ccgcgtccag ggccgggtgt tgaccacgtg cgccggcgtg
     4801 agccacgccc gccgggccac ggcgacgccg tagcgcatca tcgcgaggtg ctcgacctta
     4861 tgggcagccg tgttgatggc caggtccaca ccgcgcctgc cggcctccct ggcgagcgtg
     4921 tcgttgaggt cgagccggtt gggggaggcg ttgatctcga gcgccacccg ccgccgcgcg
     4981 gcggcggcga acacctcgtc ccagcccgcc ggatacgcct cgcgctcccc caacagccga
     5041 cccgacggat ggccgatcac atccacgtag ggatggtcga tcgcggcgac gagcctcctc
     5101 gtgatccgct gtgtcgtttc ggcaaccgcg gaatggatcg aggctacgac gatgtccatc
     5161 gtccggagga tctcatccgg cagggccagg tggccgtcgg cccggatgtc cacctccaca
     5221 ccctccagca gctgaatgcg tccttcgtac cggcgctttg cggccgcgat ctcgttgtgg
     5281 cgctcggcta cccgcgcggc atcgagcccg cgcgtgatgc ccacgccctt cgtgcgatcc
     5341 gtgatggcca ggtactcata gccgcggtcg atggccgcgc gaactactgc gtcgatctga
     5401 tcgtgcccgt ccgaccaggt ggagtggctc tgcagatccc cgcggatgtc gcgctcttcc
     5461 acgaggtccg gcaggccgtg ccggagcgcc gcctcgatct ccccctggtt ctccctcagt
     5521 tccggcggga tccagtccat ccccaggaac ttgtagacgt cttcttcgtg cgcgaaggtg
     5581 tggagtcgat cgtccttctc gatcccgtat tccgagatgg agaatccgcg ctccatcgcc
     5641 caggtgcgca gccgggcgtt gtgctcctcg gagccggtga agtgctgcag gcgagagccg
     5701 tactgctcgg cggggacgat ctcgaggtcg acccgcacgc catcggcgtg gatgatggcg
     5761 gctttggtga ctcccgcgcc caggacttgc ttcgcgcggg gatgcgagac gaatcgcccg
     5821 atcgcctgct cgggatccct ggtggcgcag acgatgtcga tgtcaccgac cgtctcgcgc
     5881 atccggcgca gcgatccgac agggtcggcg ctgaccaccc ccgggcactc ccgtagggcc
     5941 tccaccaggt cccgggcgat tggctcggcg accgggaggg ggatctggcc ggaccgtcgt
     6001 cgcagcaatg cgatgcccgc cttgatccgc tgccggatct tctctcggac ctgcacggcg
     6061 gcgatcgggg tgtcgatgac gcgttcgagg tcctcgatgc tgctgaccgc gagggcccgg
     6121 tagagttcca tggcggtttt cgggccgatg tttgggatcc tggtgagcgc gcgcgccacg
     6181 ggcgggatct tccgtgcgat ccggttgtag tatttgagat gtccggtggt gaccagttgc
     6241 tcgatcatgc tggcgatcgt ggggcccact cccgggatgg tctcgagcgc cctccgccct
     6301 ccgtaacgat agaggacgcc cgcatcttcg gccgagagct cgatggatcg ccccgcgttc
     6361 cggtaggcca tcgcgcggaa caggttttcc cctcggatct cgaggatgtc cgcgatgtcg
     6421 tagaagattc tggcgatcgc cttgttggtt acgcgattct tctccccgac gcttccaggg
     6481 ggacctcggt gcatcgtcgc tctccctcga cctaaggatc gctcaactcg gaccggacga
     6541 ccatcgggcc ggagcccgat cctgaggcga tcgcatagtg aggcctgcgg gtgtcggtgg
     6601 cggggtggcg gcacggacgg gctaatgcgg gttagggccg cccggcactc ctggtgaata
     6661 gccaccggat cgcgctctgc gggaagtcgc gctcccgcca cgtaaacgcc acgcggggcc
     6721 ggagggcgta cgtcaccccg gggtctccgt cggttccggg cctccaccga tacttcgcct
     6781 cgtaggcatc cgcgaaccgc gcgagcatcg atgcgctgga cacctcgtcc gcgtcaccct
     6841 ctaggatgac aacgtcgtcg ccgctctcga ggtgcacgac gacccgggga ttctcggcaa
     6901 ggttgcgccc cttgcgagat ccccgggcgg tggcgaaata aaacgtccca tccagccaca
     6961 cgccccagac gggaacggcg tgcggccggc caccggcccc ggccgtcgcg acccagtagt
     7021 tgtgggcggc ggccagcgca cggttgacat cgtcccaggc ggggtgcggg gcgccgctca
     7081 actgcggcga gatcctgtaa ctctccggca tggatggacg cgttggcctg gggtcctccg
     7141 atccgagcat gaccctcaca cccctccgct tccctcatgg gggcccggcc ccgcccggac
     7201 tccgcgccgg tgcctccaga ggaaccacgc cccccagacg atcagcgcca gggccgccat
     7261 ctgaccccag gacaggaaca ccaccgtccg gtcctcgctg cgcagcaggt cgagggcgaa
     7321 ccgcagcgcc gacgaggcga tgatcaccgg ccagaccagg gcgccgtcga acgcgcgccg
     7381 tcttgccgcc caaagcgccc accccaagat cacggcggtc ccggcgatct cgtagagctg
     7441 gagcgggtgg cgggggcctc ccgggacccc cgggaagacg gtcccccagg gaagcgcggt
     7501 cgggtccccg tagagctcgc cgttcatgaa gttgccccac cgcacgagca tgatcgccag
     7561 gaggaacgcc ggcgccatcg catcggccag ccgccacagg ggcacccggt ggcgcgggca
     7621 ccatacggcg agatacacca gggccacggc gatggctccg tgtgaggcca gccccgcgta
     7681 gggcggtcgg atgatcgcta gcggatcgcc gacgaaatcc tgcgggtgcg agaggacgaa
     7741 gcccgcccgg gcccccacca gcaggctcag gagaaacggc agggccagcg aatcgacgag
     7801 gaggcggcgc tcgatcccga agagcccgcc gaagtgggcg gtcaccggga tcgagaggat
     7861 gagcgccagc cccatcatcg cgccgtacca gcgcagcagg accgggccca gcacgagatg
     7921 gcccagcttg agcggcccca gccgcaagag gacggggtgc atgcacgttc agtataggac
     7981 cggcgtctta ttgacacaac aagacgtgta tgctaactta atttccaaac tgaatagcaa
     8041 ctcttatcca gagcggttga gggacgggcc cgatgaaacc cggcagccta ccaggcgccc
     8101 acctggtgtg gtgctaattc ccgcggaaga tccagttccg gtagataaga gggacgagat
     8161 cccctcgcgg aattggcggg ggggtttttg ttgtgcgcac aagtcgcctg gcgggcgcag
     8221 gcacagatgg aggggagaat ggcggcaaac gggctggtct gccgcgagtg cgggcagcgc
     8281 ttcccggcag aagcgatctt cgtgtgcgag agctgcttcg gaccgctcga ggtggagtac
     8341 gacctcggcg ggctgggcgg gggggcgctc cgggagcgga tcgccggtgg cccggcctcg
     8401 atctggcggt accaggacct gcttcccgtc ccccgggtgc ccgagtggga cctgagcccc
     8461 ggcttcaccc ccctcgttcg ggcccatcgc ctcggcgagg cgctcgggct ccggcacctg
     8521 tacgtgaaga acgacacccg aaacccgacc tggtcgttca aggaccgggt cgttgccgtc
     8581 ggcctcgcgg ccgggaagca gtttgggttc gcggtgttct cgtgcgcgtc gaccgggaac
     8641 ctggccaacg ccgtcgcggc gcacgccgcc aaagcgggcc tgcg
//
LOCUS       DASQGH010000182         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6475, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000182 DASQGH010000000
VERSION     DASQGH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6475"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VEW91_03505"
     CDS             complement(<1..593)
                     /locus_tag="VEW91_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90687.1"
                     /translation="MTGLSAGRIFGLLLRQLYLYRRSMIRMLEVVYWPVMDLLVWGFV
                     SVYVGRLRGGGALAIAFLLGGMILWDIFFRAQQAISVSFLEDIWTRNLINVFVSPIST
                     AEFIAAMLLLGVMKVIVTGVLLSVLAYALYAFNLFQYGLLLIPFVVNLLLSAWGIGII
                     TTAMILRYGQGAETLAWAIPFLFTPFSAVFYPVAVLPA"
     gene            complement(590..1351)
                     /locus_tag="VEW91_03510"
     CDS             complement(590..1351)
                     /locus_tag="VEW91_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90688.1"
                     /translation="MLPVTQDRAVTDVIWVQHLTKRFGAVTVVDNLSFGVAAGEIVGF
                     LGPNGAGKTTTLAMLLGLLLPTSGQVRLLGLPMPAERQRILARVNFTSPYVALPGNLT
                     VDENLRVFARLYGVRDPGARIGRLLERFGLAPMRRRPTGRLSSGESTRLGLVKALLND
                     PEVLLLDEPTASLDPDSAERVRESLRAIRAERRMTIFYTSHNMQEVERLSDRILFLHQ
                     GRLIADGPPEDVLRTFARDTLEEMFLDVARHGARP"
     gene            1386..>2026
                     /locus_tag="VEW91_03515"
     CDS             1386..>2026
                     /locus_tag="VEW91_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM90689.1"
                     /translation="MTAAPALDATLAREDLHAAGTVARAAEGVGFAALWANETKHNPF
                     VQLALAAVATSRILLGTGVAIAFPRSPTVMAHLAWDLAALSGGRFILGLGTQVKAHIE
                     RRFGVPWDPPVAKLREYLEAVRAVWRSWQEDLPLRVEGRYYRLSLMPPFFNPGPIAHP
                     EIPVYIAGVNPLLCRLAGETAQGFCVHPFHTVPYLTSVVIPNIARGLEARGRPR"
BASE COUNT          359 a          702 c          688 g          277 t
ORIGIN      
        1 gccggaagga ccgcgaccgg gtagaacacg gcggagaagg gcgtgaacag gaaggggatc
       61 gcccacgcca gggtctcggc cccctgaccg taccgcagga tcatcgcggt ggtgatgatc
      121 ccgatccccc acgccgacag caagaggttc accacgaacg ggatgagcag gagcccgtac
      181 tggaagaggt tgaacgcgta cagtgcgtag gcgaggacag agaggagcac cccggtcacg
      241 atcaccttca tcaccccgag cagcaacatc gccgcgatga actcggcggt gctgatcggg
      301 ctcacaaaga cattgatcag attccgggtc cagatgtcct cgaggaacga gacggagatc
      361 gcctgttggg cccgaaagaa gatatcccac aagatcatgc cgccgagcaa gaaggcgatc
      421 gccagcgccc cgcccccccg cagccggccg acgtagacgc tgacgaaccc ccacacaagc
      481 agatccatca ccggccagta gacgacctcc agcatccgga tcatgctgcg ccggtagagg
      541 tacagctgcc gcagcaggag gccgaagatg cgcccggcgg agaggccggt cacggccgcg
      601 ccccgtggcg ggccacatcg agaaacatct cctcgagcgt gtcccgggcg aaggtgcgga
      661 gcacgtcctc cggggggccg tccgcgatga ggcggccctg gtggaggaac aggatgcgat
      721 cgctcaaccg ctcgacttcc tgcatgttgt gcgaggtata gaaaatcgtc atccgccgtt
      781 cggcccggat cgcccgcagg ctctcgcgga cacgctcggc gctgtcggga tcgaggctcg
      841 ccgtcggttc gtccagcagg agcacctcgg gatcgttgag gagggccttc accaggccga
      901 gccgcgtgct ctcccccgac gagagccgac cggtggggcg gcgacgcatg ggggcgagcc
      961 cgaaccgctc caggagccgt ccgatccgcg ccccgggatc acgaacgccg tacaggcggg
     1021 cgaacaccct caggttctcg tcgaccgtca ggttccccgg cagggccacg tacggggacg
     1081 tgaagttgac gcgcgccagg atccgctgcc gctccgccgg catcggcagc ccgagcagcc
     1141 ggacctggcc ggacgtcggc agcaggaggc cgagcaacat cgccagggtc gtcgtcttcc
     1201 ctgcgccgtt gggcccgagg aacccgacga tctcgccggc ggccacgccg aacgacaaat
     1261 tgtcgacaac cgtgacggcc ccgaaccgct tggtgaggtg ctgaacccag atcacgtccg
     1321 tgacggcacg atcttgcgtg acgggcaaca cgactgcatt atacactctg gggaggaggt
     1381 gatccgtgac ggccgcgccc gcgctcgatg ccaccctcgc cagagaggat ctccacgcag
     1441 ccggcacggt cgcccgggcg gccgagggcg tgggattcgc cgccctgtgg gccaacgaga
     1501 cgaaacacaa cccgttcgtg cagctcgcgc tggcggccgt cgcgacctcc cgcatcctgt
     1561 tggggaccgg cgtggcgatc gccttccccc gcagcccgac cgtgatggcg cacctcgcgt
     1621 gggatctggc cgcactgtcc ggcggccggt tcatcctggg gctggggacg caggtcaagg
     1681 cgcacatcga gcgccggttc ggcgtgccgt gggacccgcc ggtggcgaag ctgcgcgagt
     1741 atctggaggc ggtgcgggcg gtgtggcggt cgtggcagga ggatctcccg ctccgcgtgg
     1801 agggccgata ctaccggctg agcctcatgc ccccgttctt caaccccggc ccgatcgccc
     1861 accccgagat ccccgtgtac atcgccgggg tcaaccccct cctctgccgg ctggccgggg
     1921 agacggccca gggcttttgc gtccacccgt tccacacggt gccctacctc acatcggtcg
     1981 tgatcccgaa catcgcgcga gggctcgagg cccggggccg gccccg
//
LOCUS       DASQGH010000183         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46141, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000183 DASQGH010000000
VERSION     DASQGH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46141"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VEW91_03520"
     CDS             complement(<1..1162)
                     /locus_tag="VEW91_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90690.1"
                     /translation="MTAYCAAFIAGIDANRTVKDELAAAVARRGGRRRIAVTDLVNPR
                     QAYFSRVRPEIQPSPDHLQAMMAGTGFHEVFTRSVSTEEFVEQLVEFEEIVGKIDVFE
                     DAPVELKTTGSMPADVVVDRPSHVEQLAMYCTMVGKPLGHLLYYRRAEYGRTPLLRAL
                     DLEVRGPEEVAREMVRRRDLLRHALDTGDYAALPRCEWAGRGCRFEAVCGCTTADVLV
                     RAVSGSAVVVRENPALTQRVRERIGSAPEVMPARRLGLNDLVFPRKAAFRLAAEGQVE
                     EEDDAETRMASWERRGFEEVLNDAIWYGFPGASRRVRISMGALRPSVLLFRDVPTVLR
                     TTGRREMIERGRLTAEAPHYIDRLGFECALVGSEKGRLIVYYSAIPDDKFMVY"
     gene            1382..>1692
                     /locus_tag="VEW91_03525"
     CDS             1382..>1692
                     /locus_tag="VEW91_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90691.1"
                     /translation="MDRVQTNGGMAGQVAAVLLALLLILFMSSGLDPQAASDPAKAIP
                     ILAEKHGLFVMLGILAALTSGFGLVFTIGLFSRLREPAPTRASTALGLAVVGLAGHAL
                     GV"
BASE COUNT          272 a          597 c          543 g          280 t
ORIGIN      
        1 cgtacaccat gaacttgtcg tcggggatcg ccgagtagta cacgatgagc cgccctttct
       61 cgctgcccac gagcgcgcat tcgaacccga gccggtcgat gtagtgcggc gcctctgcgg
      121 tgaggcggcc ccgctcgatc atctcacggc gcccggtggt gcgcaggacc gtcggcacat
      181 ctcgaaagag caggacggac ggtctgagcg cccccatgga gatgcgaacg cgcctggagg
      241 cgccggggaa gccgtaccag atcgcgtcgt tgaggacctc ctcgaacccg cgccgctccc
      301 acgaagccat cctggtctcc gcgtcgtcct cctcttcgac ctggccctcg gccgcaagcc
      361 ggaacgccgc cttccgggga aacaccagat cattgagccc gagccggcgg gccggcatca
      421 cctccggcgc cgatccgatc cgctcgcgca cccgctgggt cagggcggga ttctcccgga
      481 ccaccaccgc gctcccggag accgcgcgca ccagcacatc ggcggtggtg cacccgcaga
      541 ccgcttcgaa ccggcagccc cggccggccc actcacaccg gggaagcgcc gcatagtcgc
      601 ccgtgtccaa tgcgtggcgc agcaggtccc ggcgccgcac catctcgcgg gcgacctcct
      661 cggggcccct gacctcaagg tcgagcgccc ggagcagcgg cgtccggccg tactccgctc
      721 gccggtaata gagcaggtgg cccagcggct tgccgaccat ggtgcagtac atggcgagct
      781 gctcgacgtg gctgggtcga tcgacgacga cgtcggcggg catcgatccg gtggtcttga
      841 gttccacggg agcgtcctcg aacacgtcga tcttgccgac gatctcctcg aactcgacca
      901 gttgttcgac gaactcctcg gtggaaacgg accgagtgaa gacctcatgg aaccccgtcc
      961 cggccatcat cgcctggagg tgatcgggag agggctggat ctccggccgc acccggctga
     1021 aataggcctg gcgggggttg acgagatcgg tgaccgcgat cctccgccgg cccccgcggc
     1081 gggcgaccgc agcggccagc tcgtccttga cggttcggtt tgcgtcgatc cccgcgatga
     1141 aggccgcgca gtacgcggtc aacagcggtc tcccttccac gaggctgttg ggtgccaccg
     1201 tcattatacg ggaacgcagg agtatccggg cagtactacg aaatgctcac ccgcatcggg
     1261 cgttgggctc atgtgaactg atgctcaagc cccctctcat cgaagacgcg ccctgcatgt
     1321 tcttgctccc gcatcccagc gccgcgtcat ttcaagatac gaaccacaat ggaggtgctg
     1381 gatggatcgg gtccagacca atggaggcat ggcaggccag gtggccgccg tcctccttgc
     1441 cctactcctc atcttgttca tgtcgagcgg cctcgatccc caagcggcct cagacccggc
     1501 caaggcgatc ccgattctcg cggagaaaca cgggctcttc gtcatgcttg ggatcctggc
     1561 cgcgctgacc tccggcttcg gactggtctt tacgatcggg ctcttcagcc gcctgcgcga
     1621 gccggcgccg acccgggcat ccactgcact cggcctggcg gtggtggggc tcgcggggca
     1681 cgcgttgggg gt
//
LOCUS       DASQGH010000184         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_802, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000184 DASQGH010000000
VERSION     DASQGH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VEW91_03530"
     CDS             complement(<1..300)
                     /locus_tag="VEW91_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HYM90692.1"
                     /translation="MSVLATLQSEAQGEHVYGHGGWAAVAANLVTKLYLPSLADADAE
                     RLSRVMGTAAGEDVARSRGWGPMGSRTGEHRRAIPVPLERPEELRGIASSPDEILV"
     gene            complement(310..1593)
                     /locus_tag="VEW91_03535"
     CDS             complement(310..1593)
                     /locus_tag="VEW91_03535"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HYM90693.1"
                     /translation="MAISREAAEILRSIPLDHVPAAGRTELGKWIYACAAAAHCRQAL
                     VWAAGQAASAYAPTLRIAFGFLVFWIACAVLCRPRMPLGRSATGSARYALPSELRRLT
                     VRSGDAWLPLGYLPPPGWLRGLLRFHPTCRVFRALVGPQVYLPAEDLARHMLIVGLTG
                     AHKTTAVTFPVLLEAARHGVSVVALDLKYGEADGLARVASEWQRRARDVLVFAPLEPA
                     TLRWNPLDRLRTMGDAHQLAAHLFDEPDPSDPDLVYWMGAERHVCAVLCFALTTDGGA
                     ATLGRLRSLCESGPAAVYTFVRAHPATASLVGRMGAYLAMLPKDQAGILQGIASRLEA
                     WGDEAVCQATGPSAPWEQIDLARVRREPVLLVVGVPQAALGRLRWLCHLFLRDLAAYL
                     LRPRGPEEQVRVLLVLEELPVWGALPGLADHLATY"
     gene            complement(1678..2181)
                     /locus_tag="VEW91_03540"
     CDS             complement(1678..2181)
                     /locus_tag="VEW91_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90694.1"
                     /translation="MLLVGVPSTGQPIPAGETAAVLSFIDAASGHMEIMRHWPWAPAD
                     VDVIPFVPYFWSGCPGPGAIMMLITAPGIGPPLSDRIATALTTRFPGSKASRQSLELA
                     RIAAALRNPGDPVQEALKAAVPWISDLRNYERWGYAVFLFIRWQGISCGVVFTTVPEG
                     YGIPFVN"
     gene            complement(2265..>2934)
                     /locus_tag="VEW91_03545"
     CDS             complement(2265..>2934)
                     /locus_tag="VEW91_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90695.1"
                     /translation="WALAVLLGVILLGVFLFALGFMVYLAILFFAHLTLLLAIFLAPL
                     ALALLAAPGTARWTGRWAVVIVKTGLVVFSVRVIHAAALYLAVIVPVRQISSGLTSGF
                     QGLGGGSGRDPAALGVLLLNLAWLLVLMIVGTGIGVYAMLRAERLTGQFVDGVAFGEG
                     IFAGPRWLGGHVAAWYSRQASDVGPPSLGGVDHRPGPQGPWSTSGGGAQEATTAKPGG
                     RVSL"
BASE COUNT          576 a         1032 c          940 g          386 t
ORIGIN      
        1 cacgaggatc tcgtccgggg atgaagcgat cccacgcagt tcttcaggac gctcgagggg
       61 aacgggaatg gcacggcggt gctcacctgt tcgggacccc atcggacccc acccccggct
      121 tcgagccacg tcttcgccgg ccgccgtgcc catcacccgg ctcagccgct cggcatcagc
      181 atctgccagt gagggaaggt aaagcttcgt caccaggttg gccgccaccg cggcccagcc
      241 gccgtgcccg tagacgtgtt ctccctgggc ttcgctctgc agcgtggcga gcaccgacac
      301 ctggcgactt cagtatgttg ccaggtgatc agccagtccg ggaagcgccc cccacaccgg
      361 gagctcctcc aggaccagca ggacccgcac ctgctcttcg ggccctctcg gacggagcag
      421 atatgccgcc aggtcccgaa ggaagaggtg gcacaaccac cggaggcgcc cgagcgctgc
      481 ctgaggcacg ccgaccacta gaaggacagg ctcccggcgc actcgcgcga ggtcgatctg
      541 ctcccacggc gcggacggtc ccgtcgcctg gcaaaccgcc tcatcccccc atgcttcgag
      601 gcgggaggcg ataccctgaa ggatcccggc ttgatccttg gggagcatcg cgaggtaggc
      661 acccattctg ccaacgagtg atgcggttgc gggatgggcc cgcacgaacg tgtacaccgc
      721 ggcgggcccg gattcgcaca gggagcggag ccgtcccagt gtggcggccc ccccatcggt
      781 cgtgagggca aagcacagca cagcgcagac gtggcgttcc gctcccatcc agtacaccag
      841 gtcaggatcc gatggatccg gctcatcgaa gaggtgagcg gcaagttgat gagcgtctcc
      901 catcgtcctt aggcggtcaa gcgggttcca acgcagggtc gcgggctcga ggggggcgaa
      961 caccagaacg tcccgcgccc gtctctgcca ctcagaggcg acccgcgcga ggccgtcagc
     1021 ttccccatac ttgaggtcca atgcgacaac ggaaactcca tgtcgcgccg cttccagaag
     1081 taccggaaac gtgacggccg tcgtcttgtg ggctcccgtg agcccgacga tgagcatgtg
     1141 gcgggcaagg tcctcggcgg gaaggtacac ctgaggcccc accagcgccc ggaacacccg
     1201 acaggtcgga tggaacctga ggaggccccg gagccagccc ggcgggggca gatatccaag
     1261 cggaagccac gcatcgcccg atcgtaccgt tagccggcgg agttcgctcg ggagcgcgta
     1321 ccgggcgctt cctgtcgcgg agcgcccaag aggcatccgg gggcggcaga gcaccgcgca
     1381 ggcgatccag aataccagga agccgaaagc gatgcgaagc gtgggggcgt acgcgcttgc
     1441 ggcctggcca gcggcccaga ccagcgcctg gcggcaatga gcggcggcgg cgcaggcgta
     1501 gatccacttt cccagttccg tccggccggc agcgggcaca tgatcgaggg ggatcgagcg
     1561 taggatctcc gccgcctccc tggagatggc catcgcaacg acggcgacca cccctcccgg
     1621 cacaagcaag gtggccgtcc aaatcaacgc agcgcgggag ccggagtgca ttccccctca
     1681 gttgacgaac gggatcccat agccttcggg gacagtcgtg aagacgacgc cacaggaaat
     1741 cccctgccat cgaataaaga ggaacactgc gtacccccat cgttcatagt ttcggagatc
     1801 gctgatccac ggcaccgccg ccttcagcgc ctcctgaacc ggatcccccg ggttccgcag
     1861 cgcggccgcg atgcgcgcga gttccaggct ttgccgcgat gccttggaac ccgggaagcg
     1921 tgtggtgagc gccgtggcga ttctatcgga cagcggcggg ccgatcccgg gcgcggtgat
     1981 gagcatcatg atcgcacccg gccccggaca cccgctccag aagtagggca cgaagggaat
     2041 aacatccaca tctgccggag cccacggcca gtgccgcata atctccatgt ggccgcttgc
     2101 ggcgtcgatg aacgaaagca ccgccgccgt ttcaccggcg gggatcggct gcccggtgga
     2161 aggcactccc acaagcagca tcagggcacc gattcccccc acgataaaca aacgtctcaa
     2221 ggagccgccg tggctgggcg cagggcgggg cccccgtact ggcatcacag cgacacccgc
     2281 ccgcccggct tcgctgtcgt cgcctcctga gcgccgccgc ccgaagtgct ccacggtccc
     2341 tgagggccgg gtcgatgatc caccccaccc aggctcgggg ggcccacatc cgaggcctga
     2401 cgcgagtacc aggcggccac gtggcctcca agccaccggg gccctgcgaa gatcccctcg
     2461 ccgaacgcca caccgtccac aaactgacct gtcagtcgct ccgcgcggag catcgcatat
     2521 acgccgatcc cggtccccac gatcatcaag acgagaagcc acgcgaggtt caggagcagc
     2581 acaccaagcg cagccggatc tcggccagag cctcccccca gcccctggaa accgctggtg
     2641 agcccggagg agatctgccg gaccggcacg atcaccgcaa ggtagagggc agccgcatgg
     2701 atcacgcgta cgctgaacac cacaagcccg gttttcacga tcaccaccgc ccaccgcccc
     2761 gtccaccggg cggtcccggg ggcggccagg agcgccaggg cgagcggcgc caggaagatc
     2821 gccagcagca gcgtcaggtg ggcaaagaac agaatggcca ggtacaccat gaaccccaaa
     2881 gcaaagagga agacgccaag aaggatcact cccagaagca ccgcaagcgc ccag
//
LOCUS       DASQGH010000185         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_806, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000185 DASQGH010000000
VERSION     DASQGH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1513)
                     /locus_tag="VEW91_03550"
     CDS             complement(<1..>1513)
                     /locus_tag="VEW91_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYM90696.1"
                     /translation="IVRGRKGEYRQLFDDLRRQGFARVRIDGSLYELTEQIALDKNRK
                     HQIEVVVDRLVVKPEIRGRLNDSVETALKLGQGIVGISVVDGEELTFSQNFACPDDGV
                     SLPEIEPRIFSFNAPYGACPTCTGLGFKQEIDPDLVLAPDLSLLDGAVLPWASSTSEY
                     YQELLRALADAHRVDMHTPVRRLPREFLRVLLRGAEEKIRIKYHNRWGALRQYDTTFE
                     GVIPYLERRYQETDSEYMKEEIEQYMMTAPCPDCRGTRLKKESLAVTVGDRSIAEACA
                     LTVRGAQHFFAHLTLTDRERLIAHQVLKEITARLGFLVNVGLDYLTLDRTANTLSGGE
                     AQRIRLATQIGSGLMGVLYVLDEPSIGLHQRDNRRLIDTLMGLRDLGTTIVVDEHDEE
                     TIRSADWVVDIGPGAGADGGHVVAAGPLERILATPGSITGQYLSGKRSIPIPPRRRAG
                     TGQALTIRGAREHNLKRIDVRIPLGMFVCVTGVTGSGKSTLIDDILIHALAHHL"
BASE COUNT          214 a          520 c          533 g          246 t
ORIGIN      
        1 ggaggtggtg ggccagcgcg tggatgagga tgtcgtcgat caacgtggac ttccccgacc
       61 cggtgacccc cgtgacgcag acgaacatcc cgagcgggat ccggacatcg atccgcttga
      121 ggttgtgctc gcgcgcgccg cggatggtga gcgcctggcc ggtgcccgcc cgccggcgcg
      181 gggggatggg gatcgaccgc ttgccggaga gatactgccc ggtgatcgac cccggggtcg
      241 ccaggatgcg ctcgagcggc cccgcggcca cgacgtgccc gccgtcggcg cccgcgccgg
      301 ggccgatgtc caccacccag tccgccgacc ggatcgtctc ctcgtcgtgc tcatccacca
      361 cgatcgtggt cccgagatcc cgcaggccca tcagggtgtc gatcagccgc cggttgtccc
      421 gctggtgcag gccgatgctg ggctcgtcga gcacgtacag cacgcccatg agcccggacc
      481 cgatctgggt cgcgagccgg atccgctgcg cctccccgcc cgacagcgtg ttggcggtcc
      541 ggtcgagggt gaggtagtcc agcccgacgt tgacgaggaa gccgagccgg gcggtgatct
      601 ccttgagcac ctggtgggcg atcagccgct cccggtcggt cagggtgagg tgggcgaaga
      661 agtgctgcgc gccccgcacc gtgagcgcgc aggcctccgc gatcgatcgg tcccccacgg
      721 tgaccgcgag gctctccttc ttgagccgcg tcccccggca gtcggggcac ggcgcggtca
      781 tcatgtactg ctcgatctcc tccttcatgt actcggaatc ggtctcctgg taccggcgct
      841 ccaggtaggg gatcacccct tcgaacgtcg tgtcgtattg gcggagcgcg ccccagcggt
      901 tatggtactt gatccggatc ttttcctcgg cgccccgcag gagcacccgg aggaactcgc
      961 ggggcagccg gcgcaccggc gtgtgcatat cgacccggtg ggcgtccgcg agggcccgga
     1021 ggagctcctg gtagtactcg ctcgtggaac tggcccaggg cagcaccgcg ccatcgagca
     1081 gcgagaggtc gggggccagc acgaggtcgg ggtcgatctc ctgcttgaac ccgagcccgg
     1141 tgcaggtggg gcaggcgccg tacggggcgt tgaacgagaa gatccgcggc tcgatctccg
     1201 gcaggctcac cccgtcgtcg gggcaggcga agttctggct gaacgtcagc tcctccccgt
     1261 ccaccaccga gatgccgacg atgccctggc cgagcttgag ggcggtctcc accgagtcgt
     1321 tcagccggcc gcggatctcc ggcttgacca ccaggcggtc gacgacgacc tcgatctggt
     1381 gcttccggtt cttgtcgagc gcgatctgct cggtgagctc atagagggat ccatcgatgc
     1441 gcacccgggc gaacccctgc cggcggaggt cgtcgaagag ctgccggtac tcccccttgc
     1501 gcccgcggac gat
//
LOCUS       DASQGH010000186         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12173, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000186 DASQGH010000000
VERSION     DASQGH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12173"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..695
                     /locus_tag="VEW91_03555"
     CDS             210..695
                     /locus_tag="VEW91_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90697.1"
                     /translation="MRKSVSRRDVLAMAAGGLGGALVAGPLGRASAAEQQVMLYVFRG
                     EQGILGPNGKGHDAFVPSGFVVKAGVPVHLVVINYDEGPHTITAPDLGLNIAITPGKT
                     VGSNVEPVTTTYSFTPSKRGPHRWYCAFTCDGGGGGWAMQQGYAGPSKEGYMAGYIVV
                     L"
     gene            927..1547
                     /locus_tag="VEW91_03560"
     CDS             927..1547
                     /locus_tag="VEW91_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYM90698.1"
                     /translation="MVLLAAVLVVAAAGGLLWTAWNVTRPRGLTGGVFASPTAAPDFR
                     LADQDGHPVSLSGLRGKVVVLTFLYTHCPDACPLIADALHRAYGQLGSTSARTAFLAV
                     SVDPNGDTPQAIHQFLDEHHVQGELMYLHGTLAQLRPVWAHYYVGSDANKVNPAAVAT
                     AAPAPGQINHTAIVYVIDPRGRLRAFLAGDTLDPQTLVTDVKALAP"
     gene            1741..2268
                     /locus_tag="VEW91_03565"
     CDS             1741..2268
                     /locus_tag="VEW91_03565"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HYM90699.1"
                     /translation="MIRVIARVVRWALAVGVLVAAGAMAAGMPVVRAATHSADLTILG
                     GKKGGGSMEFNGYQRGAMTVTVPVGWQVVVHFENADILPHSLAVLPTGAHQQVAPPGA
                     PAFPGATTANFSAGLPKGAKQTFTFEASKAGTYEFVCGVPGHAVAGMWDSLVVSATAD
                     APSVTPPGAATVSVH"
     gene            2353..>2882
                     /locus_tag="VEW91_03570"
     CDS             2353..>2882
                     /locus_tag="VEW91_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90700.1"
                     /translation="MASRSGRGWSSWTPNINILRWFVGPRAALLGTLALAIAFLWIPP
                     ADALPSFAAQTGAPCSACHVGAFGPQLTPFGISFRANGYTLEGGTGPWASIPVNLVIS
                     PSYQTDAKAQPTAPTGYGTNNFFNILGSGTSLFIAAGHSFDGNFGIGGFEQVGFSVVP
                     GASFSASEATSDLKIT"
BASE COUNT          492 a          885 c         1021 g          484 t
ORIGIN      
        1 ggatgagacg tgagaaaagg cgagggtagc aattaaatgg agctggtggt gtccagccgg
       61 aggcagaggc gatggaacgc gagcccagag tgaccgcagc gggtcttccc agaaatcacc
      121 aggacgctct tgcgactggt ccccgaaggc gtgagatgcg aaggcggcat aaccacttcg
      181 agcgtttaga ggagacggga gggttatcga tgcgcaagag tgtgagccgg cgagatgtct
      241 tggccatggc ggcgggaggg ctgggcgggg cgctagtggc cgggccgctc ggccgggcga
      301 gtgccgcgga gcagcaggtc atgctatacg tcttcagagg tgagcagggc atcctgggcc
      361 cgaacggaaa gggccatgac gcgtttgtgc cgtccggctt tgtcgtgaag gccggcgtcc
      421 cggttcacct ggtggtaatc aactacgacg aagggccgca cacgatcacc gcgccggatc
      481 ttgggctgaa tatcgcgatc accccaggca agacggtcgg ttctaacgtg gagcctgtga
      541 ccacgaccta ctccttcact ccgtccaagc gagggccgca ccgctggtac tgcgccttca
      601 cgtgtgatgg agggggcggc ggatgggcga tgcaacaggg ctacgcgggt ccgagtaaag
      661 aaggctacat ggccgggtat atcgtcgtgc tgtaaataga ccaccggtcc agtgcggacc
      721 aggcaagccg gggcggagcc cttcgagatc gcggcgttac gcgcagtggg tgaggctccc
      781 gcgctcattc ccgcgggatc ggcgtgaggg ttcgccggac ccgttggtca gcggcgatcc
      841 aacgtaggaa gggaggtgag gcagtgaacg atgtgaggtc cgcggcgacg ccttctcctg
      901 gtccgcgggc ccgcgggccg cgacgcatgg tcttgctcgc cgcggtcctg gtcgtggcgg
      961 ccgccggagg tctcctctgg acggcgtgga atgtgacccg cccccgggga ctaaccggtg
     1021 gggtcttcgc ttcgccgaca gccgcgcctg acttccgcct ggcggaccag gacggtcacc
     1081 cggtctccct ctcgggcctg cgcgggaagg tggtggtgct cacgttcctc tacacgcact
     1141 gcccggacgc ctgcccgcta atcgcggacg cactccatcg ggcgtacggg cagttgggga
     1201 gcaccagcgc tagaaccgcg ttcctggcgg tgagcgtgga tccgaacggc gacacccccc
     1261 aggcgatcca ccagttcctg gacgagcacc acgtgcaggg agagctcatg tatctgcacg
     1321 gcacgctcgc gcaactgcgg cccgtctggg cgcattacta cgtcgggagc gacgcgaaca
     1381 aagtcaaccc cgcggccgtc gcgaccgcgg cgccggctcc gggtcagatc aaccacaccg
     1441 cgatcgtcta cgtcatcgac cctcgaggga ggctgcgggc cttcctggcc ggagacaccc
     1501 tcgaccccca aaccctcgtc acggacgtga aagcgctggc gccgtagggc atgccgtccc
     1561 gccgcgctcg gagggtaccc gatagacacg gtcggccgcg gcctgctcgc ctgtcggcga
     1621 cgacgatgac gggccggggt tttcccgagg gggcaaggaa ggacggcgag cgggctcggt
     1681 ttgagatgga aatctcggtc agaccggcgc ggaggtgatg atgtggcgca ggaacacgga
     1741 gtgataaggg tcatcgccag ggtggtgcgg tgggcgttgg cggtcggggt cctcgtagcg
     1801 gcaggagcga tggccgcggg gatgccggtt gtgcgcgccg cgacgcactc ggccgactta
     1861 acgatcctcg ggggcaagaa gggtggcggg agcatggagt tcaacggata ccagcgtggc
     1921 gccatgacgg tcacggttcc cgtgggctgg caggtcgtgg tgcactttga gaacgcggac
     1981 attctccctc acagtctggc tgtgctgccc accggcgcgc accagcaggt ggcgccacca
     2041 ggtgcaccgg cgttcccggg cgcgacgacc gcgaatttct ccgcggggct ccccaagggt
     2101 gccaagcaga cgttcacgtt cgaggcgagc aaggccggca cctacgagtt tgtctgcggc
     2161 gtaccggggc acgccgtcgc cgggatgtgg gacagcctgg tcgtttcagc gacggcggac
     2221 gcgcccagcg tgacgccgcc gggcgctgcg acggtcagtg tgcactagct cgtccccgcg
     2281 ggcctcggcg tcccgctacc agggtagagt acggagcggt atgtgagcgc accacgagag
     2341 ggggggcagg ccgtggctag ccggtctgga cgtggatggt cctcttggac acccaacatc
     2401 aacattctgc gctggttcgt tggtccgcgc gccgctctcc tcggtaccct ggcgctcgcc
     2461 atcgcgttct tgtggatccc gcccgccgat gccctaccaa gtttcgccgc gcaaacaggt
     2521 gcgccatgca gcgcctgtca tgtcggtgcg ttcgggcctc aactgacgcc gttcggcatc
     2581 tccttcagag cgaacggcta taccctggaa gggggcaccg gcccttgggc gagtattccg
     2641 gtcaatctcg taatttcacc gagctaccag accgatgcga aggcccagcc gacggcgccc
     2701 acgggctacg gcacgaacaa ttttttcaat attctcggaa gcggaacgtc tctcttcatc
     2761 gcggcagggc attccttcga tggcaacttt ggcatcggcg gatttgaaca agtggggttc
     2821 agtgtcgtcc caggggcatc gttcagcgcg tctgaggcaa cgtcggatct caagatcacg
     2881 aa
//
LOCUS       DASQGH010000187         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3659, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000187 DASQGH010000000
VERSION     DASQGH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3659"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VEW91_03575"
     CDS             complement(<1..186)
                     /locus_tag="VEW91_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90701.1"
                     /translation="MDLAPDVVPSVLGRLAVEAPAGYSQVLAELAGSQIRLDSEDELR
                     RRQRRGAPRRLLFSWGEY"
     gene            350..>1928
                     /locus_tag="VEW91_03580"
     CDS             350..>1928
                     /locus_tag="VEW91_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM90702.1"
                     /translation="MSPASARDTQRLPPGALRWTCDPAGLEFKTTGDLPKEAAIVGQD
                     RGVHALQFGLGIDQPGYNIFVSGPPGTGRSSYSRWEIERLAQARPVPPDWCYVRNFAA
                     ADQPIGISLPPGEGVTFGERLAEMVAEVRAGLRRAFASEAYERGRTEVARRYEQQLGE
                     VMQQLENQAKARGLMLQRTPTGIITIPVDLQGRPISQEVFEALPEAERARIAARTKAL
                     EEVMADAQRRARALEREGREALREFDAATARGVIDSPIAPLKEQYRDHPKVVAFLDAV
                     ERDMLAHLTELRAAGDEEAGEQPRIELPFPRRDPYARYQVNLLVDHRGARGAPVIFET
                     NPTYYNLVGKAEYRAEFGALVTDFTMIKSGALHRASGGYLILQARDVLTNPLAYDGLK
                     RALRDRAIRIEPLGEALGLLPAATLRPDPIPLDVKVVLIGTPEVYHLLYTYDEEFEKL
                     FKIRADFDVVIDRTPDAQRAYARAIAAICHRTGACHFDPSGVAAMLEHSARLASSQDK
                     LSTRFNEVVEIIHEAAAW"
BASE COUNT          319 a          684 c          660 g          265 t
ORIGIN      
        1 gtactccccc cacgagaaca acaggcgcct cggcgcgccg cgtcgctgtc ttctgcggag
       61 ttcatcctcg ctgtccagcc gaatctggga cccagcgagc tcggccaaca cctgcgagta
      121 gcctgcgggc gcttccaccg cgagccggcc gagcacgctg ggcacgacgt cgggggccag
      181 gtccacatca ttcccaaggg cctcccgaat ccgctgggct gtctctgggc tgagcgagat
      241 cctcatcggc gcaaagtgta tgcgatattt atatatccag acatcagtat atccagggcg
      301 caggtcctgt caagaggcga acccctgctc cccagcgaac tgtgcggcta tgtcccccgc
      361 atccgcgcgc gacacccagc gccttccccc gggagcgctc cgctggacct gcgacccggc
      421 cggactggag ttcaagacga ccggcgacct gccgaaggag gccgcgatcg tcggccagga
      481 taggggcgtg cacgccctgc agttcggact ggggatcgac cagccgggct acaacatctt
      541 tgtgagcggg ccgcccggca cgggccggag cagctactcc cggtgggaga tcgagcggct
      601 cgcccaggcg cgcccggtgc cgccggactg gtgctacgtc cggaacttcg ccgccgcgga
      661 ccagccgatc gggatctcgc tgcccccggg ggagggggtg acgtttgggg agcgcctggc
      721 cgagatggtc gccgaggtgc gggcggggct ccggcgggcg ttcgccagcg aggcctacga
      781 acgggggcgc accgaagtcg ccaggcggta cgagcaacag ctcggggagg tcatgcagca
      841 actcgagaac caggcgaaag cgcgcggcct catgctccag cgcacgccca ccggcatcat
      901 caccatcccc gtggatctgc aggggcgacc gatctcccag gaggtgttcg aggcgcttcc
      961 ggaggccgag cgggcgcgca tcgccgcgcg cacgaaggcc ctcgaggagg tgatggccga
     1021 cgcgcagcgc cgggcgcggg cgctcgagcg cgagggccgc gaggccctgc gcgagttcga
     1081 cgcggcgaca gcgcgggggg ttatcgacag cccgatcgcg cccctcaagg agcagtaccg
     1141 cgaccacccg aaggtggtag cctttctcga tgcggtcgag cgcgacatgc ttgcccacct
     1201 gaccgaactc cgcgcggcgg gcgacgagga agcgggcgag cagccgcgca tcgagctgcc
     1261 gttccctcgg cgggatccgt acgcccgcta ccaggtcaac ctgctcgtgg accaccgggg
     1321 ggcgcgcggc gcgcccgtga tcttcgagac gaacccgacc tactacaacc tggtcggcaa
     1381 ggccgagtac cgcgccgagt tcggggccct cgtcaccgac ttcacgatga tcaaatcggg
     1441 cgcgctgcac cgggccagcg gcggatacct cattctgcag gcgcgggacg tcctcacgaa
     1501 ccccctcgcc tacgacggcc tcaaacgggc cctgcgggac cgggcgatcc ggatcgagcc
     1561 tctgggggag gcgttgggac tcctgccggc ggcgacgctg cggcccgatc cgatcccgct
     1621 cgacgtcaag gtcgtgctca tcggcacccc cgaggtctac cacctcctct atacgtacga
     1681 tgaggagttc gagaagctgt tcaagatccg ggccgacttc gacgtcgtga tcgaccgcac
     1741 gcccgacgcc cagcgcgcct acgcccgggc gatcgcagcg atctgccacc gcacaggggc
     1801 ctgccacttc gatccctcgg gggtggcggc catgctcgag cacagcgccc ggctggccag
     1861 cagccaggac aagctcagca cccgcttcaa cgaggtggtc gagatcatcc acgaggccgc
     1921 ggcctggg
//
LOCUS       DASQGH010000188         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_812, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000188 DASQGH010000000
VERSION     DASQGH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VEW91_03585"
     CDS             <1..772
                     /locus_tag="VEW91_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HYM90703.1"
                     /translation="IPHYVKSHVHPQNLESGEMPLIPNFRIPVQIHTRSANAKWLDEI
                     AHTNPLWIHPSDAARVEVETGDLVRIETEIGHFVVKAWVTEGIRPGVVACSHHMGRWK
                     LGEEGQRQMMATVSLDHHEHRWGMRRRSGAHPYASGDPDTLRIWWTDVGVHQNLTFPV
                     HPDPVSGQHCWHQAVRVHKAGLNDRYGDIFVDTSISGKVFHDWLARTRPADRYSPNRT
                     RRPYWLMRPLRPTRDAYRLPPAPLNGEERGAPAGVAGE"
     gene            772..1644
                     /locus_tag="VEW91_03590"
     CDS             772..1644
                     /locus_tag="VEW91_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HYM90704.1"
                     /translation="MELLRALATLAEPPGSEHAHLGGLLGLPGAPEPALYTEVFAFNL
                     YPYASVYVGREGMLGGEARDRVAGFWRALGRVPPVEPDHVTALLALYATLAEQEAADP
                     DPASRLLWRQSRKALLWEHLASWLFVYLDKLGEVAPPFYKSWGALLGEALVAEIQVVG
                     PQEALALHLRLAPALPDPRQDGAEEFVGALLSPVRSGVLLTRADLTRAARDLGVGVRM
                     GERRFILTSLLSQDAEGMLGWLTAEARGWAARHRTHGWVTGRVARFWAGQAASAAALL
                     EALHRSAGRTAESV"
     gene            1777..3051
                     /locus_tag="VEW91_03595"
     CDS             1777..3051
                     /locus_tag="VEW91_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HYM90705.1"
                     /translation="MDPETHSRILGAVEERRGEIIAFLQRLIQYDSVTGNETEIQAFI
                     AAHLEGLALEVDRFDADPNVLRQYPGFLEPEKPMAGRPNVVGVWRGRGGGRSLLLNGH
                     VDTVPLEPVSEWGRGPLSGALADGSVWGRGASDMKGGVAAMTMAVAVLKDLGLQPHGD
                     VILESVVDEERTGLGTLACVHRGYRADAGICCETSDLEVMPACIGRMWFTIHVRGKPA
                     GISARWEGVSAIDKAMKLVGAVEALEAMRIQDLRHPLYPDNRGALPCAVTMFHAGTFP
                     SITPEEATLRGSMGLMPYEDPAQVEAQLRAQIMRVCDADPWLRHHPAELTTKGGYVAA
                     GAEIPTDHPIVDTVGRSFRQVTGREPVLGARMGASDTRFLIRQGHTPTVIFGPGTTSQ
                     MHAMNESIPVENVVIATKVLALAIHTWCNQPG"
     gene            complement(3061..3822)
                     /locus_tag="VEW91_03600"
     CDS             complement(3061..3822)
                     /locus_tag="VEW91_03600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM90706.1"
                     /translation="MAYTAAELLAATASRLLADNKIVFAGVGTPLLASVLAKSTHAPH
                     LTIVVEGGVVGLEVLPGRLPISTNEMRAGRRAVMLPSITQTFLYAQRGFFDYGFLGGA
                     QIDAYGNINTSVIGPPDRPRVRLPGSGGACDIITHCREIFIVTTHERRRFVDRVDFIT
                     SPAYLGGRDARHQAGLLFGEITTVITDLALMRFDPDTKRMRLDALQAGVTIDQVRAQT
                     GFDLAIAPEPAGLPAPTGEELRILRMLDPDRLFLG"
     gene            complement(3824..4795)
                     /locus_tag="VEW91_03605"
     CDS             complement(3824..4795)
                     /locus_tag="VEW91_03605"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM90707.1"
                     /translation="MTPPPLRSRSATFVEARRRIEAKDRSLRDKRMTAHDAARLVRDG
                     DHVAIGGCLYSRTPLVTLREILRAGRADLTLSRNLMCYEGELFLVAGATRTLVTSWIG
                     IGLPWGLSRITREFVEGGQARLEEWSHLALGLRYRAAAMGLPFLPTLSMLGSDLLGRA
                     GARTMTCPFTGETLCLVPALFPDVAVIHVHRADGFGNAQIDGYPHMDADLAAAAATVI
                     LSAEEIVSTDEIRRTADRTVIPFFTVDAVVEAPFGAFPHECYGRYEADLDHIGAYARD
                     AAGGGAVAVRAYLDEYVFGPETFEAYLERFGADRLRRQQQAAWELTR"
     gene            complement(4792..>5287)
                     /locus_tag="VEW91_03610"
     CDS             complement(4792..>5287)
                     /locus_tag="VEW91_03610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM90708.1"
                     /translation="RSVMLPKPFQRPRPLMLFGGYVDAVLRRIARYSDGWLTYFYTPE
                     SFRKTWARIRSLTEEAGRDPAGLRNVSQLPIYVAPTFEEADRGVRDFIARYFDVAPWS
                     ESTADSSIRGTPDQCVEQLAAHVAAGVEHIVLVPCDYRLDQLDVIGKEILPRFRGRVA
                     GVTA"
BASE COUNT          834 a         1775 c         1910 g          768 t
ORIGIN      
        1 gatcccgcac tacgtcaaga gtcacgtgca cccgcagaac ctggagagcg gcgagatgcc
       61 gctcatcccg aacttccgca tcccggtgca aatccacacg cggtcggcca acgccaagtg
      121 gctcgacgag atcgcccaca ccaaccccct atggatccac ccatccgacg ccgcccgggt
      181 ggaagtcgag acgggggacc tcgttcggat cgagaccgag atcggccact tcgtggtcaa
      241 ggcctgggtc accgagggca tccggccggg tgtcgtggcg tgcagccacc acatgggacg
      301 gtggaaactc ggcgaggagg gccagcggca gatgatggcg accgtgtcgc tcgaccatca
      361 cgagcaccgc tgggggatgc ggcggcgcag cggcgcccac ccctacgcct ccggggatcc
      421 ggacacgctc aggatctggt ggaccgatgt cggcgtgcac cagaacctca cgttccccgt
      481 ccatcccgac ccggtgtccg ggcagcactg ctggcaccag gcggtgcggg tccacaaagc
      541 cggactcaac gaccggtacg gggacatctt cgtcgacacg agcatttccg ggaaggtctt
      601 ccacgactgg ctggcgcgca cgcggccggc cgaccggtac tccccaaacc gcacccgccg
      661 cccgtactgg ctcatgcgtc cgctccgccc gacccgggac gcctaccgcc tcccgcctgc
      721 ccccctcaat ggggaggagc gcggagcgcc ggcgggcgtc gccggagagt gatggagctc
      781 ctccgcgcgc tggccaccct cgcggaaccc ccggggtccg agcacgcgca cctcggcggg
      841 ctgctgggcc tcccgggggc cccggagccc gccctctaca ccgaggtgtt cgcgttcaac
      901 ctctacccgt acgcctcggt ctacgtcggg cgcgaaggga tgctgggggg tgaggcccgg
      961 gatcgggtcg ccggattctg gagggccctc ggtcgcgttc cgccggtcga gccggaccat
     1021 gtcaccgcgc tgctggcgct gtatgcgacg ctggccgagc aggaggcggc ggatcccgat
     1081 cccgcctccc gtctcctctg gcgccagagc aggaaggcgc tcctgtggga gcacctggcc
     1141 tcctggctct tcgtgtacct ggacaagctg ggggaggtcg cgccgccgtt ctacaagtct
     1201 tggggcgcgc tcctcggaga agccctcgtc gcggaaatcc aggtcgtggg accccaagag
     1261 gcgctggcgc tccaccttcg cctggccccg gcgctcccgg acccacggca ggacggtgcg
     1321 gaggagttcg tcggggccct cctctccccc gtacggagcg gggtgctcct cacgcgggca
     1381 gacctgaccc gggcggcccg cgacctcggg gtgggggtca ggatgggaga gcgccggttc
     1441 atcctcacgt ccctgctctc gcaggacgcg gaggggatgt tggggtggct caccgcggag
     1501 gcccgcgggt gggcagcgcg gcaccgcacg cacgggtggg tgaccggccg ggtggctcgt
     1561 ttctgggccg ggcaggccgc cagtgcggcg gcgctgctgg aggcactcca ccgatcggcg
     1621 gggcggactg cggagagcgt ataaggcagt gggcgcgcgc ggcatcgcga ggaggctccc
     1681 catggtcacg gaattgcgga tcttcaccat cagcaagggc cggttggacg attggttcct
     1741 cacgccggtg ctcccgcagg cggccagatg aaggcgatgg atcctgagac gcacagccgg
     1801 atcctcggcg ccgtggagga gcggcggggt gagatcatcg cgtttctcca gcgcctgatc
     1861 cagtacgaca gcgtcaccgg aaacgaaacc gagattcagg ccttcatcgc cgcgcacctc
     1921 gagggactcg ccctcgaggt tgatcggttt gacgccgacc cgaacgtcct gcggcagtac
     1981 cccgggttcc tcgagccgga gaagccgatg gccgggaggc caaacgtggt gggcgtctgg
     2041 aggggccgcg gcggcggccg ctcactgctg ttgaacggcc acgtcgacac cgtgccgttg
     2101 gagcccgtga gcgagtgggg gcggggaccc ctgtcgggcg ccctcgccga cggcagtgtg
     2161 tggggacggg gcgcctcgga tatgaaaggc ggagtggcgg cgatgaccat ggcggtggca
     2221 gtcttgaaag acctcgggct gcagccccac ggcgatgtca tcctcgagtc cgtggtggac
     2281 gaggagcgaa cgggtctcgg gacgctcgcg tgcgtgcacc gagggtaccg ggcggatgcc
     2341 ggaatctgtt gcgagaccag cgacctcgag gtcatgccgg cgtgcatcgg ccggatgtgg
     2401 ttcacgatcc atgtccgagg caagccggcc ggcatctcgg cacggtggga aggggtgagc
     2461 gccatcgata aggccatgaa attggtgggg gccgtggagg ctctggaagc catgcggatt
     2521 caggaccttc ggcatcccct ctatcctgac aaccgagggg cgctcccctg cgcggtcacg
     2581 atgtttcacg cggggacgtt cccgagcatc acgcccgagg aagcgaccct gcggggcagc
     2641 atgggattga tgccgtacga agatcccgcg caggtcgagg cgcaactgcg ggcgcagatt
     2701 atgcgcgtct gcgacgcgga cccctggttg cggcaccacc ccgcggagct aacgaccaag
     2761 gggggctacg tggcggcggg ggcggagatc ccgaccgacc atcctatcgt ggacacggtc
     2821 ggccggtcgt tccggcaggt gacgggacgg gaaccggtgc tgggcgcccg catgggagcc
     2881 tccgacaccc gctttctgat ccggcagggg cacaccccga cggtgatctt cggcccggga
     2941 acgacgagcc agatgcatgc gatgaacgag tccattcccg tggaaaatgt ggtcatcgcg
     3001 accaaggtgc tggcgctcgc catccacacc tggtgtaatc agccggggta gccgcccccg
     3061 ttaccccagg aacagtcggt ccggatcgag catcctgagg atgcgcaact cctcgccggt
     3121 cggggccggg agcccggcgg gctcgggggc gatcgccagg tcgaaccccg tctgtgcccg
     3181 gacctgatcg atcgtcacgc ccgcctggag tgcatcgagg cgcatccgtt ttgtgtccgg
     3241 gtcgaagcgc atcaaggcca ggtcggtgat gaccgtggtg atctctccga ataacagccc
     3301 ggcctggtgg cgggcatcgc ggcccccgag gtaggcgggg ctggtgatga agtcgacccg
     3361 atcgacgaag cggcgacgct cgtgcgtcgt gacgatgaag atctcccggc agtgggtgat
     3421 gatgtcgcag gcgcccccgc tgcccggcaa gcgaacgcgg ggccggtcgg gcggcccgat
     3481 cacgctggtg ttgatattgc cgtaggcatc gatctgcgcg ccgcccagga agccatagtc
     3541 gaagaacccg cgctgcgcgt agaggaaggt ctgcgtgatg gacggaagca tcaccgctcg
     3601 acggccggcc cgcatctcgt tggtggagat cggcaggcgc ccgggcagga cttcgagtcc
     3661 gacgaccccg ccttccacca cgatcgtcag gtgcggcgcg tgggtcgact tggccaggac
     3721 ggacgcgagg aggggcgtgc cgacgccggc aaacacgatc ttgttgtcgg cgagcagccg
     3781 gctcgcggtc gcggccagga gttcggcggc ggtgtaggcc acgtcagcgc gtgagctccc
     3841 acgcggcttg ctgctgacgg cgcagccggt cggcgccgaa gcgctccagg tacgcctcga
     3901 aggtctccgg gccgaagacg tactcgtcca ggtacgcgcg gaccgcgact gcgcccccgc
     3961 ccgccgcatc ccgcgcgtag gccccgatgt ggtccagatc cgcctcatag cggccgtagc
     4021 attcgtgcgg gaaggcgccg aatggggcct cgacgaccgc gtccacggtg aagaacggga
     4081 tcaccgtgcg gtccgcggtc cgccggatct cgtcggtgga gacgatctcc tccgcgctga
     4141 ggatcacggt ggcggcggcg gcggccaggt ccgcgtccat gtggggatag ccgtcgatct
     4201 gggcgttgcc gaagccgtcg gcccgatgga cgtggatcac cgccacgtcc gggaagagcg
     4261 cgggcaccag gcacagcgtc tccccggtga acgggcaggt catcgtgcgg gcccccgcgc
     4321 gaccgagcag gtccgagccg agcatcgaga gcgtggggag gaacgggagc cccatcgcgg
     4381 cggcccgata ccgcaacccg agggcgaggt ggctccactc ctcgaggcgg gcctgcccgc
     4441 cttcgacgaa ctcccgggtg atccgcgaca ggccccacgg caggccgatg ccgatccagg
     4501 aggtgacgag cgtccgggtg gcccccgcaa ccaggaagag ctccccttcg tagcacatca
     4561 ggttgcgcga gagggtcagg tcggcgcgcc ccgcccgcag gatctcccgg agcgtcacga
     4621 gcggcgtccg cgagtacagg cacccgccga tggcgacgtg gtccccatcc cgcacgagtc
     4681 gcgccgcatc atgcgccgtc atgcgcttgt cgcgcagcga acggtccttc gcttcgatgc
     4741 ggcgccgcgc ctcaacgaac gtcgcggacc ggctgcggag cgggggcggg gtcacgccgt
     4801 cactcccgcg acccttcctc gaaagcgcgg caggatctcc ttgccgatca cgtcgagctg
     4861 atccaggcgg tagtcacacg gcaccagcac gatatgctcg acgccggcgg cgacgtgggc
     4921 ggcgagctgc tccacgcact gatccggggt cccacggatc gagctatccg cggtggactc
     4981 gctccagggg gcgacgtcga agtaccgcgc gatgaaatcc cggacgcccc ggtctgcctc
     5041 ttcgaaggtg ggcgcgacat agatcgggag ctggctcacg ttccggagcc cggcgggatc
     5101 ccggccggcc tcttcggtga gcgatcggat cctggcccac gtcttgcgaa agctctcggg
     5161 cgtgtagaag taggtcagcc acccgtcgct gtaccgggca atgcggcgga gcaccgcgtc
     5221 gacgtagccg ccgaagagca tgagcggccg cggccgctgg aagggcttgg gcagcatcac
     5281 cgatcgg
//
LOCUS       DASQGH010000189         4788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51693, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000189 DASQGH010000000
VERSION     DASQGH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51693"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VEW91_03615"
     CDS             <1..230
                     /locus_tag="VEW91_03615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYM90709.1"
                     /translation="PSYPDKLWTEAEGTIEAPERVMRVTAIRVKYHLAIPPGKREDAE
                     RALAVFERGCPVAQTLKGCVAITHEWEIREG"
     gene            247..471
                     /locus_tag="VEW91_03620"
     CDS             247..471
                     /locus_tag="VEW91_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HYM90710.1"
                     /translation="MTYGFQCRSCRHEFEITASVAEYEARPTPACPRCGHRQTRRVFT
                     PVLIMSAAGKAGPGDAPGGCCGGGACGCRH"
     gene            557..1900
                     /locus_tag="VEW91_03625"
     CDS             557..1900
                     /locus_tag="VEW91_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HYM90711.1"
                     /translation="MLRERLDAREEAGTPVRVGVIGAGRFGTMVICQLAAMRGMRPSV
                     VADLDGARGMQALVHAGFPVDAVVQADAVGRVDAAIARGAPALTEDPEVLLRSAVDVV
                     VEATGVPEVAARSATEAFRRGKHVVMVSVEADVLVGSVLRRMADQAGVVFSAAYGDQP
                     ALIHELVEWARVLGFQVVAAGKGTKYLPEYRKGTPDDIWERYGADPARREGLNPQMYN
                     SFTDGTKSAIEMAAVANMTGLRPDVRGMHFPPAGIAELAEVLKPREAGGVLHRSGVVE
                     VVSSLDRDGRPVPNDLRWGVYVVITSPQPYLRRCFAEYGMPVDRTGAYAAMYRPYHLV
                     GMELPISIARAALDRRATGAPLPVPVAAVVCAAKRPLASGEVLDGEGGYTVYGIIDDA
                     ASAWRERLLPMGLSRGARVVHPVAEDGLVRLDDVAVDTSSALYALWEQQTHLLSR"
     gene            1906..2493
                     /locus_tag="VEW91_03630"
     CDS             1906..2493
                     /locus_tag="VEW91_03630"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HYM90712.1"
                     /translation="MHRVHASGEIYPFRTLPDYLRAGLDLVFVGINPGLYSVQQGHYF
                     ARPTSRFWPAFSRSRLSARVRAALGRDVLRPEDDRALLAFGIGFTDVVKAPSRSASDL
                     SPAHFRRWAPRLLARLEAHQPRVACFHGLTAYRGFARYALHAVRADWDLGVQPLRVIR
                     TRLVVVPNPSPANAHFRPADYVRWYDRIAALLRAD"
     gene            2830..4302
                     /locus_tag="VEW91_03635"
     CDS             2830..4302
                     /locus_tag="VEW91_03635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYM90713.1"
                     /translation="MSLVRVLVLLALIPALWFGIIKVTAANRIAVLLGVYIILLAWRP
                     RWLPLLRKPDLVVMTDLMVTTLLVVVSGNLNSPFLYVYYLTILEAAARFNLRQALAAS
                     TATTGMIIFLWIETGQAEAIITPGFRLGAFIAGGFLLALFSGVLAQELRTVRERMQWA
                     SLLDQRLQEATGRLEEQLRELQFNNDLAARLAGELQVKGVLEILLRALLEIVGLGRGI
                     AYLCDGEGKPHLIVTHGVASEGSQQPSDAGALTLPADATGGELILHQGRPADASRDAI
                     VASVPLVRGTHLRAWLCGLGDSPVAIPDSVRQSIRGLAAMGASTLDAARLHEQMRDQA
                     ATDALTGLANRRSFIDRLTAELARVSRTDRRLSVAMLDLNGFKSINDTYGHAVGDGVL
                     TKVARQLARSVRVVDLVARFGGDEFVLLFPEMEREHAEKTLARLRIDDITAPDGGRVL
                     PLSLSWGIATCPQDGIEINALLHAADLRLYAMKERIQNPK"
     gene            complement(4347..4727)
                     /locus_tag="VEW91_03640"
     CDS             complement(4347..4727)
                     /locus_tag="VEW91_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90714.1"
                     /translation="MGTQKGSSMGRLRAPLVLTAVYVILLALATLSPFVVRIVFGYAV
                     KDPGVLLVLSAAFWGSGVILWAIASYPESYSTLAGPVIVYLLIFIIFLLWGWHGRFYT
                     PQNITLPLIINSVLVIWIWLVRKR"
BASE COUNT          739 a         1585 c         1693 g          771 t
ORIGIN      
        1 tcccgagtta ccccgataag ctgtggaccg aagccgaggg aaccatcgaa gctcccgagc
       61 gggtgatgcg ggtgacggcg atccgtgtga agtaccacct ggcgatccct ccagggaaac
      121 gggaggacgc cgagcgcgcg ctggcggtgt tcgagcgggg ctgcccggtc gcgcagacgt
      181 tgaagggatg cgtggccatc acgcacgaat gggagatccg cgaaggctga gtcggagggg
      241 gcgtcgatga cgtacgggtt ccagtgccgg tcttgcaggc acgagttcga gatcaccgct
      301 tcggtcgccg agtacgaggc gcgccccacc ccggcgtgtc cccggtgcgg ccaccggcag
      361 acccggcggg tctttacccc cgtcctcatc atgagcgcgg cggggaaggc cgggccgggg
      421 gatgcgccgg gggggtgctg cgggggtggc gcgtgcgggt gccgccactg agcagcccgt
      481 tcttgcggga gtggagcgtg gagatgcgga gccggccggg acgtcccggc cggctcgctg
      541 attgtgcggg ccggcggtgt tgagggaacg cctggacgcg cgcgaggagg ccgggacccc
      601 cgtgcgcgtc ggggtgatcg gcgcagggcg gttcgggacg atggtcatct gccagctggc
      661 cgcgatgcgg gggatgcgtc ccagcgtcgt ggccgacctc gacggggcgc gggggatgca
      721 ggcgctcgtg cacgccgggt tcccggtgga tgccgtggtg caggccgacg cggtcggccg
      781 ggtcgacgcc gcgatcgccc gcggggcccc ggcgctcacg gaagaccccg aggttctcct
      841 ccgcagtgcc gtcgatgtcg tcgtcgaggc gacgggcgtg cccgaggtcg cggcgcgctc
      901 tgccaccgaa gccttccgcc ggggcaagca cgtggtgatg gtgagcgttg aagccgacgt
      961 gcttgtgggg tcggtgctgc ggcggatggc cgaccaggcc ggggtcgtgt tttctgcggc
     1021 atacggggac cagccggccc tgatccacga gctggtcgag tgggcccgcg tcctcgggtt
     1081 tcaggtcgtg gccgccggca agggcacgaa gtacctcccg gagtaccgca agggcacccc
     1141 cgacgacatc tgggagcgct acggggcgga cccggctcgc cgcgaggggc tcaaccccca
     1201 gatgtacaac tcgttcaccg atgggacgaa gtcggcgatc gagatggccg cggtcgccaa
     1261 catgaccggc ctgcgcccgg atgtgcgcgg gatgcatttc cctccggcgg gcatcgccga
     1321 gctggccgag gtgctgaagc cacgcgaggc cggtggggtg ctgcaccggt ccggggtggt
     1381 cgaggtggtg agctcgctag accgtgatgg gcgcccggtg cccaacgatc ttcggtgggg
     1441 ggtgtacgtg gtgatcacgt ccccgcagcc gtacctccgg cgctgcttcg cggagtacgg
     1501 gatgccggtg gaccgcaccg gcgcgtacgc ggcgatgtac cgcccctatc acctcgtcgg
     1561 catggagctc ccgatcagca tcgcgcgcgc cgcgctcgac cgccgggcga ccggcgcgcc
     1621 gctccccgtg cccgtggcgg cggtcgtctg cgccgcgaag cggccgctcg cttccggcga
     1681 ggtgctggac ggggagggtg ggtacaccgt ctacgggatc atcgatgacg cggcatcggc
     1741 ctggcgcgag cggctgctgc cgatggggct cagccgcggc gccagggtcg tccacccggt
     1801 cgccgaagac ggcctggtgc ggctcgatga tgtcgccgtc gatacctcga gcgcgctgta
     1861 cgccctgtgg gagcaacaga cgcacctgct ctcccgctga agcccgtgca tcgcgtgcac
     1921 gccagcggcg agatttatcc gttccgcacc ctgcccgact atctcagggc cgggctcgac
     1981 ctcgtgttcg tcgggatcaa tccggggctc tattccgtgc agcagggaca ttactttgcc
     2041 cggccgacca gccggttctg gcctgccttc tcccggtcgc gcttgagcgc ccgcgtccgg
     2101 gccgcccttg ggcgggatgt gctgcgcccc gaggacgacc gggccctcct cgcgttcggc
     2161 atcgggttca ccgatgtggt gaaggccccg agccggagcg cgagcgacct gtcaccagcc
     2221 cacttccgca ggtgggcgcc caggctgctg gcgcgtctcg aggcccacca gccgcgtgta
     2281 gcctgctttc acgggctcac cgcctaccgc ggatttgcgc gctacgccct gcacgccgtg
     2341 cgggcagact gggacctggg tgtgcaaccc ctgcgggtga ttcggacccg gctggtcgtc
     2401 gtacccaatc ccagccccgc gaacgcccac ttccggccgg ccgattacgt ccgctggtac
     2461 gatcggatcg cggcgctgct gcgggccgac taacgccccg ccagcattag cgcaatatca
     2521 tgtggccggt ggcccacaag atcagggccc ccattaacag gatcacgaac cacatctcgc
     2581 ggcctccctc gccaacctag tctgggacct tgcattggag tgtatgccca ctcccccggc
     2641 gcgccggacc tcctggcagc atgcctgctt gcggctgacc tcgtctggcc ataggttcga
     2701 gtgggcatta gcctaggaaa gacgttccga gggaaggtgc gcatggctcc caaagaccac
     2761 ggaggagcgt tgccgacccc accacctcca ctgtccactt ccacggtcac ccccttaaac
     2821 gagggctggg tgagcctggt gcgggtcctg gtgctgctgg cgctgatccc ggcgctgtgg
     2881 ttcgggatca tcaaggtgac ggcggcaaac aggatcgcgg tgctgctggg cgtgtacatc
     2941 atcctgctgg cgtggcgccc tcggtggctg ccgctgctac gcaagcccga tctcgttgtg
     3001 atgacggatc tcatggtgac gacgctcctg gtcgtcgtct ccgggaacct gaacagtccg
     3061 ttcctctacg tgtattacct gacgatcctg gaggccgctg cccggttcaa tcttcgccag
     3121 gccctcgcgg cctcgacggc aacgaccggg atgatcatct tcctctggat cgagacagga
     3181 caggccgagg cgatcatcac gccgggattc cgcctcggcg cgttcatcgc cggcgggttc
     3241 ctgctcgcgt tgttctccgg cgttctcgcc caggagctgc ggaccgtccg ggagcggatg
     3301 cagtgggcgt cgttgctgga ccagcggtta caggaagcca ccggccggct ggaggaacaa
     3361 ctgcgcgaac tgcagttcaa caacgacctg gcggctcgcc tcgccggcga actgcaggtg
     3421 aagggcgtgc tggagattct cctgcgggcg ttactcgaga tcgttggcct cgggcgcggg
     3481 atcgcctacc tctgcgacgg ggaggggaag ccgcacctca tcgtgacgca cggggtcgca
     3541 tccgaaggat cgcagcagcc cagcgacgcc ggggccctca cgctgcccgc cgatgccaca
     3601 ggaggcgagt tgatcctcca tcagggccgg cccgccgatg cctcacggga cgccatcgtg
     3661 gcgagcgtgc cgctcgtgcg cggcacgcac ctccgcgcct ggctctgcgg gctgggtgat
     3721 tcgccggtgg caatccccga ttccgtgcgc cagagcattc gcggcctggc ggcgatggga
     3781 gcctccaccc tggacgcggc ccggctgcac gaacagatga gggatcaggc ggcgacggac
     3841 gccctgacgg ggctggccaa ccggcggtcg ttcatcgacc ggctgaccgc ggagctcgcg
     3901 cgcgtcagtc gaacggaccg tcggctgagc gtcgcgatgc tcgatctcaa cggtttcaag
     3961 agcatcaacg acacctacgg ccacgccgtc ggcgatgggg tgctcaccaa ggtcgccagg
     4021 cagctggcga ggagcgtccg cgtggtggac ctcgtggccc gcttcggcgg ggatgagttt
     4081 gtcctgctgt tccccgagat ggagcgcgag cacgccgaga agacgctggc gcggctgcgg
     4141 atcgacgaca tcaccgcccc ggatgggggg cgcgtcctcc ccctgagcct ctcatggggg
     4201 atcgccacct gcccccagga cggcatcgag atcaacgcgc tcctccatgc agccgacctc
     4261 cggctgtacg ccatgaagga gcgcattcag aatccaaagt aagatccccg gttgccgacg
     4321 cactccggtt acgccagatc cgccgctcag cgttttctca ccaaccaaat ccagatgacc
     4381 agcacgctat tgatgatcag tgggagggtg atgttctggg gggtgtagaa gcgtccatgc
     4441 caaccccaaa gcagaaagat gatgaagatc aagagataga cgatcaccgg tcccgccagg
     4501 gtactgtaac tttcgggata actggcgatc gcccacagga tgaccccgga tccccagaag
     4561 gcggccgaca agacgagcag aacccctggg tccttgaccg cgtagccgaa cacgatgcgc
     4621 acgacaaagg gcgacaatgt ggcgagagcc aggagtatga catagactgc cgtcaacacg
     4681 agcggcgcac gaagcctccc catggaggag cctttctgcg tgcccatccg atgtcctgcg
     4741 gcgcctggac cgggcggagg ggatcgtcga gagcataggg aatcggcc
//
LOCUS       DASQGH010000190         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17828, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000190 DASQGH010000000
VERSION     DASQGH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17828"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1298)
                     /locus_tag="VEW91_03645"
     CDS             complement(111..1298)
                     /locus_tag="VEW91_03645"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HYM90715.1"
                     /translation="MREVDFSEHTVRRRWREVKGALREDLTVWTRHYLKRLLEHTLEE
                     EREVYLRAARHARTPDRQDYRNGYYARDLSTELGLLRHLRVPRTRTAGFVPTSLDRYQ
                     RRRPAVAALLREAFLTGASTRRVGPLLAPLLGERVSASTVSRITQTLDADVAVFHRRR
                     FQTPILYLLLDGIRLRVKTPTGVQQRLVLCAYGLTPAGQRRLLSFRQAKAESAAAWGA
                     FLQDLYARGLTGETLQLVVTDGSPGLHAALDIVYPYVLRQRCWVHKLRNVAAKLPRRH
                     QAACLGEVARIYAAPTERAARQAFRAWVTRWRGHAPQAVACLERDLGELLSCFASPPE
                     HRKKIRTTNAIERAFREVRRRVRPMTCFTNPASCDRIVYAVVKHLNTEWELRPLKGFT
                     QKS"
BASE COUNT          256 a          497 c          554 g          258 t
ORIGIN      
        1 ccgtgtgaaa acgggagagc ctaacattcc aagcccctca ggatgacaga atcactgaac
       61 aattcgaggc tgacagaatc attcatctac cacatcggac acgggtagtg tcaagatttc
      121 tgtgtaaatc ccttgagcgg gcgtagctcc cactcggtgt tcaaatgctt gactacggca
      181 tagacgatcc gatcgcagct cgccgggttg gtgaaacacg tcataggacg cacccgccgc
      241 cggacctccc ggaaggcgcg ttcgatggcg ttcgtcgtgc ggattttctt ccggtgctcc
      301 ggggggctcg cgaagcagct gagcaactcg ccgaggtcgc gctcgaggca ggccacggcc
      361 tggggggcgt ggccccgcca gcgggtgacc cacgcccgga aggcctggcg cgcggcccgc
      421 tcggtcggcg ccgcgtagat ccgggcgact tcccccaggc acgccgcctg gtgccggcgc
      481 gggagcttcg ccgccacatt gcggagcttg tggacccagc agcgctgccg gagcacgtag
      541 gggtagacaa tgtccagggc ggcgtgaagg ccggggctcc cgtcggtgac gaccaactgc
      601 agcgtctccc cggtgaggcc ccgggcgtag aggtcctgca ggaaggcgcc ccaggctgcg
      661 gcgctctcgg ccttcgcttg ccggaaactc aggaggcgcc gctggccggc cggcgtgagc
      721 ccgtaggcgc agaggacgag ccgctgctgc acgcccgtgg gcgtcttcac gcggagccgg
      781 atcccgtcga ggagcaggta gaggatcggg gtctggaaac ggcgccggtg gaagacagcg
      841 acgtccgcat cgagcgtctg ggtgatccgg gagacggtgc tcgcgctcac ccgctccccg
      901 aggagcggtg cgaggagcgg gcccacccgg cgggtgctgg cgccggtgag gaaggcctcg
      961 cggagcaggg ccgccaccgc cggccggcgg cgctggtagc ggtcgaggct ggtcggcacg
     1021 aagccggccg tgcgggtgcg ggggacccgg aggtggcgga ggagcccgag ttccgtgcta
     1081 aggtcgcggg cgtagtagcc gttccggtag tcctggcggt ccggcgtgcg tgcgtgtcgg
     1141 gcggcccgga ggtagacctc ccgctcctct tcgagcgtgt gctccaggag ccgcttcagg
     1201 tagtggcggg tccacaccgt caggtcctct cgcaacgcgc ccttgacctc gcgccagcga
     1261 cggcgtactg tgtgctccga gaagtccact tctcgcatgg ggtgtatcct cctctctggg
     1321 ttggcgcccg agggaagata cacctcattg ctttcaggcc tcaggggtat ttacacacga
     1381 actgatacac taccatgggg cattgttcac ttgacactgg aggacaattc ggcatattat
     1441 gttggcatgt acatcgcggt cgtccccaac cgggactcgc cccctgccat cctgctgcgg
     1501 gaaggctatc gcgaaggggg taaggtcaag aaccgcaccc tcgccaatct gagcaagtgg
     1561 cccgc
//
LOCUS       DASQGH010000191         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14990, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000191 DASQGH010000000
VERSION     DASQGH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14990"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1185)
                     /locus_tag="VEW91_03650"
     CDS             complement(43..1185)
                     /locus_tag="VEW91_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90716.1"
                     /translation="MAERGGHARGFTRIGYLDLPGGGQVVVEGRYAYVAHMSPPQGTS
                     VVDVKDPAHPRLVSQITVPDEIHSHKVRVTGEFMLVNNERFPPTDARRSPAVGLRIFD
                     ISDHSRPREVGFFPTHGRGVHRFDLQGTLGFISTEWEGFRSNILAILDISDPSRPSLV
                     GRWWLPGQDRPAHASSPGDGGHYWVHLALARGTRAYAACGRAGAVIVDVSDPRRPATV
                     GGTAWNPPYASPTHTFLPVPHLIRNRRFAVVTDEDVTDDVLEDPPAFMWVLDITNEAR
                     PVPVATYQVDPAGLARPGRRFGAHQPWEHIREDNIVFIAWFGGGIRAVDISNPYAPRE
                     AGYYVPPPATANGVPQTNDVFVDARGLVFAIDRYEGLSVLEFTPRP"
     gene            complement(1231..>1899)
                     /locus_tag="VEW91_03655"
     CDS             complement(1231..>1899)
                     /locus_tag="VEW91_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HYM90717.1"
                     /translation="AAVPAEAVQASLGCGNPTALAQLAPGEVVLDLGSGGGIDVLLSA
                     RRVGPAGKAYGLDMTDEMLALARENQRKAGVENVEFLKGEMEHIPLPDHSVDVIISNC
                     VINLSADKDRVFAEAFRVLKPGGRFAVSDVVVRGEVPAEIRRSIELWVGCIAGALEET
                     DYVAKLAKAGFEAVSIEPTRVYNVEDARGVLADHGFDVDAVAPMVRDKFMSAFVRARK
                     PATA"
BASE COUNT          312 a          665 c          628 g          294 t
ORIGIN      
        1 agtctcgtat ggccgcccgc ccccgcacca tgccggagac gttcaagggc ggggtgtgaa
       61 ctccagcacg ctcagcccct cgtaccggtc gatcgcgaag accagacccc gggcgtctac
      121 gaagacgtcg ttggtttgcg ggacaccgtt ggccgtggcg ggagggggca cgtagtatcc
      181 ggcctcccgc ggcgcgtacg ggttggagat atccaccgcc cggatgcccc cgccgaacca
      241 cgcgatgaag acgatgttgt cctcccggat gtgctcccag ggctggtggg cgccgaaccg
      301 cctgccgggc cgagccaggc ccgccgggtc cacctgatac gtcgccacgg gtacgggccg
      361 ggcttcattg gtgatgtcca gcacccacat gaacgccgga ggatcctcca gcacgtcgtc
      421 ggtgacgtcc tcatcggtca cgacggcaaa ccgtcgattc cggatgagat gcggaaccgg
      481 caggaaggtg tgcgtgggcg aggcgtacgg cgggttccac gccgtgcccc ccaccgtcgc
      541 ggggcgccgt ggatcggaca catccacaat gaccgcgccc gcccgcccgc acgccgcgta
      601 ggcccgggtc ccccgggcca gcgccaggtg cacccagtag tgcccgccgt cccccgggga
      661 tgacgcgtgc gccggccggt cctggccggg cagccaccaa cggcccacga gggaggggcg
      721 ggacgggtcc gagatgtcca ggatggccag gatgttggac cggaagcctt cccactccgt
      781 ggagatgaac ccgagggtcc cctgcaggtc gaagcgatgc acgccgcggc cgtgggtggg
      841 gaagaacccc acctcgcggg gacggctgtg gtcggagatg tcgaagatgc ggagccccac
      901 ggccggcgac cggcgtgcat ccgtcggcgg aaaacgctcg ttgttgacca gcatgaactc
      961 gccggtcacg cggaccttgt gggagtggat ctcgtccggg acggtgatct gggagaccag
     1021 gcgcggatgc gcgggatcct tcacatcgac gacgctggtc ccctgggggg ggctcatgtg
     1081 ggcgacatag gcataccggc cctccaccac cacctgaccg ccgcccggca ggtcgaggta
     1141 gccgatccgc gtgaaccccc gcgcgtgtcc tccgcgctcc gccacggtct caccccctgg
     1201 ctatcgtaca ggagtgccgt tcgcgaggcc tcacgccgtt gccggcttcc gtgcgcggac
     1261 gaatgcgctc atgaacttgt cacggaccat gggggcgacc gcatccacgt cgaacccatg
     1321 gtcggccagc acgccgcggg cgtcttcgac gttgtatacg cgcgtcggct cgatgctcac
     1381 tgcttcgaag ccggccttcg cgagtttggc cacgtagtcg gtctcttcga gcgccccggc
     1441 gatgcagccc acccacagtt ctatgctccg gcggatctcc gcgggcacct ctccgcggac
     1501 gacaacatcc gacaccgcga atcggccacc gggctttagg actcggaacg cttcggcgaa
     1561 cacccgatct ttgtcagcag acagattgat cacgcagttg gagatgatga catcgacgga
     1621 atggtccggc agcgggatgt gctccatctc acccttgaga aactcgacgt tctccacacc
     1681 ggccttccgc tgattctctc gcgccagcgc cagcatctcg tcggtcatgt cgagaccata
     1741 cgccttcccc gcggggccca cccgcctggc cgatagcaag acatcgatcc cgccaccaga
     1801 gccaaggtcg aggacgacct caccgggggc cagttgcgcc aaggcggttg gattcccgca
     1861 tcccaaggag gcctggacgg cttccgcggg cactgccgc
//
LOCUS       DASQGH010000192         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17838, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000192 DASQGH010000000
VERSION     DASQGH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17838"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VEW91_03660"
     CDS             <1..354
                     /locus_tag="VEW91_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HYM90718.1"
                     /translation="GAHFFPRRLVCARCLSLAMATVPLNTRGTLYTYTTVYQSTPEFP
                     TPYLLAYIDLPEGVRLLAQLVGVGPAEVRIGMPLELRVETVGTEAGGRPVLGYRLHAV
                     PESGPGVSIPEVNHV"
     gene            347..1489
                     /locus_tag="VEW91_03665"
     CDS             347..1489
                     /locus_tag="VEW91_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYM90719.1"
                     /translation="MSRVWIVGVGMTKFGKHPDRTAADLGAEATLEAIKDANIDPRRI
                     EGAYCGHVFQGMVTGQRVLAQTGLAGIPLVNVEDACSSGAVAIREAALAILAGEHDVV
                     LALGVEHLTGKFRGALTPGEDDLEGAVGLTMPAIYAMRARRHMADFGTTRRQIALVSV
                     KNKRNASQNPLAQIQAPVTLDEVLASRPIADPLCLQDCSPVSDGAAAAVLCSGRVIGR
                     IGAARAARLAASALRTGVVEAAPGSMAFEELTARTARAAYERAGVGPEEIHVAEVHDC
                     FSIAEILRVEGLGRYAPGEYPAALERGEAAIGGRRPINPSGGLLGKGHPLGATGVAQV
                     VELVRQLRGEAGARQVDGARVGLAHCRGGKAAGVEGAACTVQIVVA"
     gene            1506..>2700
                     /locus_tag="VEW91_03670"
     CDS             1506..>2700
                     /locus_tag="VEW91_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HYM90720.1"
                     /translation="MGEQIPISCVVMRGGTSKGIFLRASDLPPEPAARDRMILALFGS
                     PDPRQIDGLGGADILTSKVAIVGAATRPDADIDYTFGQVSITEPVVDYDINCGNLSAA
                     VGLYAVEEGLVSATEPLTTIRVHNTNTGKVFVASVPTRGSEPVIEGGCAIDGVPGTGA
                     EILLDFAGTAGAATGLLLPTGRVRDTLDVPILGGAVEVTIVDLANLCVFFRAGAVGMT
                     GAEGPREVTSQMLDAMSAVKSAAAARLGIPADGLVPLPVAVAAPATYPTYLGTTVQAA
                     SVGLVARLAGGRPPMLHKTFPGTAAACTAVAACLPGSVPWQVARPQEGGRLTIGHPGG
                     VMTVLAEVREEGGVIRVERAAYPRTARRLLEGRAFVRTARLQPAGEAALNRARVGSTP
                     GVSEGR"
BASE COUNT          390 a          911 c          995 g          404 t
ORIGIN      
        1 ggggcgcact tcttcccccg ccgcctggtg tgcgcccgat gtctgtcttt ggcgatggcg
       61 accgtccccc tgaatacccg cgggaccctc tacacttaca cgacggtcta ccaatccacc
      121 ccggagtttc ctacgccgta cctgctggcc tatatcgacc tcccggaggg cgttcgcctg
      181 ctggcccaac tcgtcggcgt ggggcctgcg gaggtgcgca ttggcatgcc gctcgagctg
      241 cgggtcgaga cggtgggcac cgaggccggc ggccgcccgg tactgggcta ccggctccat
      301 gccgtccccg aaagcggccc tggggtgagc atacctgagg tgaaccatgt ctagggtgtg
      361 gatcgtcggc gtcgggatga cgaaatttgg gaagcacccc gatcggaccg ccgcggacct
      421 cggcgccgaa gcgaccctcg aggcgattaa ggatgccaac atcgatccac gccgcatcga
      481 aggggcctac tgcggccatg ttttccaggg catggtgacc ggccagcgtg tgctggcgca
      541 gaccggcctc gccggcattc cgctcgtaaa cgtcgaggac gcctgcagca gcggtgccgt
      601 cgccatccgc gaggccgccc tcgccattct ggctggagag cacgacgtgg tgctggcgct
      661 tggcgtcgag cacctgacgg ggaagttcag gggcgcgctc accccgggag aggacgatct
      721 cgagggggcg gtcggcctga ccatgcccgc catctacgcc atgcgcgcgc ggcggcacat
      781 ggccgacttc ggcacgacgc gccggcagat cgcgctggtc tcggtgaaga acaagcgaaa
      841 cgccagccag aaccccctgg cgcagatcca ggccccggtg accctcgacg aggtgctggc
      901 gtcgcgcccg atcgcggacc ccctgtgtct tcaggactgt tcgccggtca gcgacggggc
      961 agcagcagcg gtcctgtgta gcggtcgggt gatcggccgc atcggcgcag ccagggcggc
     1021 ccggctggcg gcgtcggcgc tccggaccgg ggttgtggag gcggcccccg ggtcgatggc
     1081 gttcgaggaa ctaaccgcgc gcacggcacg ggcagcctat gagcgcgccg gcgtgggacc
     1141 ggaggagatc cacgtggcgg aggtccacga ctgtttctcc atcgccgaaa tcctgcgcgt
     1201 ggaagggctg gggcggtatg ccccgggcga gtatcccgcg gcgctcgagc gcggcgaggc
     1261 cgccatcggc ggccgccgcc cgatcaaccc cagcgggggc ctgctcggca agggccatcc
     1321 cctcggcgcc accggcgtcg cgcaggtggt cgagctggta cggcagttgc ggggtgaggc
     1381 cggcgcccgt caggtcgacg gcgcgcgcgt cgggctcgcc cactgccgcg gcgggaaggc
     1441 ggcgggcgtc gagggcgcag cgtgcacggt gcagatcgtg gtggcataac ctcgaggtga
     1501 tcgccatggg cgagcaaatc cctatctcgt gtgtcgtcat gcggggcgga accagcaagg
     1561 ggatcttcct gcgggcgagt gaccttcccc cagagccggc cgcccgcgac cggatgatcc
     1621 tggcgttgtt cggcagcccg gatccgcggc agatcgatgg cctaggcgga gccgacatcc
     1681 tcaccagcaa ggtggcgatc gtcggcgcgg ccacgcgtcc agacgccgac atcgactaca
     1741 ccttcggcca ggtcagcatc accgaaccgg tcgtggacta cgacatcaac tgtggaaacc
     1801 tgtccgccgc ggttgggctc tatgccgtgg aagagggtct tgtgagcgcg accgagccgc
     1861 tcacgacgat ccgggtgcac aacacgaaca cgggcaaggt gtttgtggcg tccgttccca
     1921 cgcgtggaag cgagccggtg atcgaaggtg gctgtgcgat cgatggggtg ccgggcaccg
     1981 gggccgagat cctcctcgac ttcgccggga cggccggggc ggccacgggc cttctgctgc
     2041 cgaccggccg ggtgcgcgac acgctggatg tgccgatcct tggcggcgcg gtcgaggtca
     2101 ccatcgtcga tctcgcgaac ctgtgcgtat tcttcagggc cggcgccgtg ggcatgaccg
     2161 gggccgaggg cccgcgcgag gtcacctcgc agatgctgga cgcgatgagc gcggtgaaga
     2221 gcgcagccgc ggcgcgcctg gggatccctg ccgatggcct cgtcccgctg cccgtggccg
     2281 tcgccgctcc ggcgacctac cctacctacc tgggcacgac ggtgcaggcc gcatcggtcg
     2341 gtctggtggc gaggcttgcc ggcggccgcc cccccatgct gcacaagacc ttcccaggca
     2401 cggcagcggc gtgtacggcc gtcgccgcgt gcttgcccgg gagcgttcca tggcaggtgg
     2461 cccgcccgca ggagggagga cggttgacga tcgggcaccc cggtggtgtg atgacggtgc
     2521 tcgctgaggt ccgcgaggaa ggcggtgtca tccgggtcga gcgcgccgcg tacccgcgga
     2581 cggcacgccg tctcctcgaa ggccgcgcgt tcgtccggac ggcgcggctg cagcccgcgg
     2641 gggaagccgc actgaaccgc gcgcgcgtcg gatcgacccc aggcgtttct gaggggaggg
//
LOCUS       DASQGH010000193         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34787, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000193 DASQGH010000000
VERSION     DASQGH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34787"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VEW91_03675"
     CDS             complement(<1..679)
                     /locus_tag="VEW91_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM90721.1"
                     /translation="MSTETIPASGIGRPAPLNRWLVTVSVLLGAMMGAIDTSVVNVAL
                     AHIQASYGVTTQEVTWVSTSYLITLVIIMPLTAWVASVLGRKRMYLLAVAVFTAASVL
                     CGFSRTLGQLIAFRVLQGLGGGVLSPTAQAIMRESFPPEEQGQAQGFFGMIILLGPAI
                     GPTLGGWLTDNYSWPWIFFVNVPIGMLALVVGSQVLVDPAYMRGRGFRRIDGAGIGLM
                     AVALASLQ"
     gene            complement(684..>2225)
                     /locus_tag="VEW91_03680"
     CDS             complement(684..>2225)
                     /locus_tag="VEW91_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HYM90722.1"
                     /translation="AAGPGRPPANKWLVTTSVLFGSMMAAIDSSVVNVALAHIQATYG
                     VTTQEVTWVSTSYLITLVIVMPLTAWVGSAFGRKRMYLLAGSIFTVASVLCGFSRTLG
                     QLIAFRVLQGLGGGALQPIAQAILRETFPVEEQAQATGWYGMAVLLGPAVGPTLGGWL
                     TDNLSWPFIFFINLPIGIMGLLIGTWFIVDPPYMRAHGLRRIDGVGIGFMAIGLASLQ
                     ILLEQGETDGWFQSDFIVMLAIIAVLALAAFIVRELRVPEPAVNLRILGNVTFASGTV
                     MTGVLGLALWGSLILLPLFLQNLLGYDATQAGLMLMPRALTMVLMMPIVGALYNRLGA
                     YVMVPFGLLLTAASGFLMGRFTLSTGPVQVLIPQIIQGVGFAFMFVALATTALSGIPR
                     PQMQSATGLYNLVRQLGGSFGTAIVITLVDHKTTTASANLMRYATIYNPTFMGWWQHF
                     QGAFMARGSDPGTAHRQALAALQGLINQQAAVVAFDYAFAMIGIVFLACLPLVLLMHR
                     GAGAR"
BASE COUNT          453 a          730 c          745 g          297 t
ORIGIN      
        1 tctggagcga tgcgagcgcc acggccatca gaccgatgcc ggcgccgtcg attcgccgga
       61 agcctcgccc gcgcatgtac gcgggatcga cgaggacctg ggagcccacc accagggcga
      121 gcatgccgat gggaacgttg acaaagaaga tccagggcca ggagtagtta tcggtgagcc
      181 agccacccag ggtgggccca atggccgggc ccaacaggat gatcatcccg aaaaagccct
      241 gcgcctgtcc ctgctcttcc ggcggaaacg actcccgcat gatcgcctgg gcggtggggg
      301 acaggacgcc accccccaac ccctgcagca cccggaacgc gatgagctgg cccagggtgc
      361 gcgagaaccc gcacaggacg gacgccgccg taaacacggc gacggccagc aggtacatgc
      421 gttttcggcc gaggacggag gccacccagg cggtcagcgg catgatgatc acgagcgtga
      481 tcaggtagga cgtgctgacc caggtgacct cctgggtcgt gacgccatag gacgcctgga
      541 tgtgggcgag ggcgacattg accacgctcg tatcgatcgc gcccatcatg gcacccaaca
      601 ggaccgagac ggtgaccagc catcggttga ggggggccgg gcgtcctatc cctgaggcgg
      661 gaatggtctc ggtggacatg ggatcagcgg gcccccgccc cgcgatgcat caacagcacg
      721 agcggcaggc aggcgaggaa cacgatcccg atcatcgcga acgcatagtc gaacgccacg
      781 acggccgcct gctggttgat cagcccctgc agcgccgcga gcgcctgccg gtgggccgtc
      841 ccgggatcgc tgccgcgcgc catgaaggcc ccctggaaat gctgccacca tcccatgaac
      901 gtcggattgt agatcgtcgc gtaccgcatg aggttggcac tggccgtcgt ggtcttgtga
      961 tccacgagcg tgatcacgat cgccgtccca aaactcccgc ccaactgccg gaccaggttg
     1021 tacagtcccg tggcgctctg catctgcgga cgggggatcc ccgaaagggc cgtggtggcg
     1081 agcgcgacaa acatgaacgc gaatcccacg ccctggataa tctgcggaat caggacctgc
     1141 acggggccgg tactgagggt aaagcggccc atcaagaagc cggaggccgc ggtgaggagc
     1201 aggccgaacg gcaccatcac gtacgcgccc aggcggttgt agagggcgcc aacgatcggc
     1261 atcatgagca ccatggtgag ggcgcgcggc atgagcatga ggcctgcctg ggtggcatcg
     1321 tagcccagga ggttctgcag gaacagcggg agcaggatca gactccccca caacgcgagc
     1381 cccagcaccc cggtcatgac ggtccccgat gcgaacgtga cgttcccgag gatgcggagg
     1441 ttcacggccg gctcggggac acggagctcc cggacgatga acgccgccag ggcgagcacc
     1501 gcgatgatcg cgagcatcac gatgaaatcg ctttgaaacc acccgtccgt ctcgccctgc
     1561 tcgagcagaa tctgcagcga cgccagcccg atcgccatga agccgatgcc caccccgtcg
     1621 atccgacgga gcccgtgggc gcgcatgtac ggaggatcga cgatgaacca cgtcccgatg
     1681 agcagcccca tgatcccgat cggcaggttg atgaagaaaa tgaagggcca cgagagattg
     1741 tcggtcagcc agccgccgag ggtcggccca accgcagggc cgagcaggac ggccatcccg
     1801 taccacccgg tggcctgcgc ctgttcctcg accggaaacg tctcgcgcaa gatcgcctgg
     1861 gcgatcggtt ggagcgcgcc gccgcccagg ccctgcagca cccgaaacgc gatgagttgt
     1921 ccgagggtcc gggagaagcc gcagagcacc gaggcgacgg taaagatcga gccggccagg
     1981 aggtacatcc gcttccggcc gaaggcggat cccacccagg cggtgagcgg catcacgatg
     2041 accagcgtga tcaagtagga cgtgctgacc caggtgacct cctgcgtcgt cacgccatac
     2101 gtggcctgaa tgtgagcaag ggcgacattc acgacgctcg agtcgatcgc cgccatcatc
     2161 gacccgaaca ggacggaggt ggtgacgagc cacttgttcg ccggcggccg gcccgggccc
     2221 gccgc
//
LOCUS       DASQGH010000194         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3696, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000194 DASQGH010000000
VERSION     DASQGH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VEW91_03685"
     CDS             <1..1773
                     /locus_tag="VEW91_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYM90723.1"
                     /translation="LGRLRESSWGATLRIVAMSALATPEDAALWLRAGCVGAIEKPID
                     VTTFAQEISRWVSGAAPEAQPSVGAPERKGEKFWEILVANGLITPEQLTRAMTAQAAT
                     GKRIGHILVEQEHVSEDDVAWAFGHQLGYPYIYPTLDIIDQEVARLLPEAFLRERHVL
                     PILKFGREMTLAMADPTDQRTVDEVVRRTGVQVNRALTLGSHIQQLLDALFAREAAAR
                     SRGVAPEAQYLQFHLVQALRQGASEIHLDPAVDAQARVRYRLHGVPVDRTGHPSELHL
                     AILQYLRHLTGLEEAPFGTAAATVQAGGTELQMVVTFLPTVAGPAASLRLYPRRADVP
                     DFAPLGLSTETIRPLSEALQGARGIVLVGCGDPVLRSTLLHGLIPSSYRGKVWTLETV
                     PSYRRATLNQTVLGSPSEAPGHLRAAASAGSDLIMVDDISAGCAAVLAAHEVARTRMV
                     LAGHPEDDVVALSSEILECVGPARVASTLRGVLVARGIRLRCPACKLPSTGGPGSLQG
                     KQTFIAQGCGACHMTGFGGQRVLAQVWCMNQEVRRLLHSGRIRAAVERITQETAAGLR
                     DQGLALVEDGLTPPDELAGIVDQV"
     gene            1773..2864
                     /locus_tag="VEW91_03690"
     CDS             1773..2864
                     /locus_tag="VEW91_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYM90724.1"
                     /translation="MTITDLLIQTKERGASDLHLKAGAPPSFRINGKLVRPDASPLSR
                     EEMHAMLYDILSDEQRARFEASHDLDFAIELKTIGRFRANAYVHRLGEGLVLRLIPST
                     IKPIEELGMPPILRDLAMQERGLVLVTGPTGSGKSTTLAAMVAHMNAHRDDHIITIED
                     PIEFIHEDRKCSISQREVGPHTESFSAALRSALREDPDIILVGEMRDLETIGQALTAA
                     ETGHLVLSTLHTNSAPQTISRVVDVFPPHQQEQIRVQVADALLGVIAQTLVPTLDGTG
                     RVAACEIMVATPAVRNLIRENKVHQLPSAIQTGVREGMQSLDQCLKNLVKTRRINNEE
                     AVKRAIEKQAFLHEGPGVRVDHPEARVQR"
     gene            2950..3624
                     /locus_tag="VEW91_03695"
     CDS             2950..3624
                     /locus_tag="VEW91_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM90725.1"
                     /translation="MRSILVVEDDPSVVKLLHLSLERDRCRVTSAADGRAALEIAQRD
                     RPDLVILDLGLPDLDGLEVCRRLRATSDVPILILTGAAEEVDKLLGLSMGADDYVTKP
                     FSPREVAARVKAILNRTTRPRQERVLRRHDLELDLERRRVLLAGQEVSVTTSEFTLLR
                     VLLESPERVFSRDELLTQIYPRDEAAVVERAIDVHIANLRRKLGDSPADPRHIATVRG
                     AGYKLL"
     gene            complement(3697..3903)
                     /locus_tag="VEW91_03700"
     CDS             complement(3697..3903)
                     /locus_tag="VEW91_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90726.1"
                     /translation="MPDGREIVGFSTDSPERMFGIRGANLLCILDEASGIPEPIFEAV
                     EGNRADGIKVIMFSSPTSTSWPAS"
BASE COUNT          743 a         1396 c         1357 g          703 t
ORIGIN      
        1 ctgggacgct tgcgagagag ctcgtggggc gccaccctgc ggatcgtagc gatgagcgcg
       61 ctggccaccc ccgaggatgc ggcgctgtgg ctgcgggccg gatgcgtcgg agccatcgag
      121 aagccgatcg acgtcaccac gtttgcacag gagatctcgc gctgggtgtc aggggcggcc
      181 cctgaggctc agccgtcagt gggtgccccg gagaggaagg gggagaagtt ctgggagatc
      241 ttggtcgcta atggcctcat cacccccgag caactgacgc gcgccatgac cgcgcaggcg
      301 gcgaccggca agcggatcgg acatatcctg gtggagcagg agcatgtgtc cgaagacgat
      361 gtggcctggg cgtttggcca ccagctcggg tacccgtata tctacccaac ccttgacatc
      421 atcgaccagg aggtcgcgcg gctcctcccc gaggccttcc tgagagagcg gcacgttctg
      481 cccattctga agttcggccg tgagatgacg ctggcgatgg ccgaccccac cgaccagcgc
      541 acagtcgacg aggtggtccg ccggaccggg gtgcaggtga accgcgcgct caccctgggc
      601 tcccacatcc agcagttgct ggatgccctg ttcgcgcgcg aagcggcggc caggagtcgg
      661 ggcgtcgctc cagaggcgca gtacctgcag ttccaccttg tccaggccct ccggcagggg
      721 gcttccgaaa tccacctcga tcctgcggtc gacgcgcaag cccgggtccg ctatcgtctc
      781 cacggcgtcc cggtagatcg gacaggccat ccgtcagagt tgcaccttgc tattctccag
      841 tacctgcgcc acctgacggg gctagaggaa gcaccgttcg gaaccgcggc cgcgacggtc
      901 caagcgggcg ggacggagct ccagatggtg gtgacgttcc tgcctacagt ggcgggaccc
      961 gcagccagct tgagactcta tccccgccgg gcggacgtgc cggattttgc gcccctgggc
     1021 ctatccacgg agacgatccg tcccctctcc gaagcgttgc agggtgctag gggaatcgtc
     1081 ctggtggggt gcggcgatcc ggtgctgcgg tccaccctcc tccacggcct gattccctcc
     1141 agctatcggg gaaaggtctg gaccctcgaa acggtgccgt cctatcgccg ggcaacgctc
     1201 aatcagacgg tccttggctc tcccagtgag gcccctgggc atcttcgggc cgcagccagc
     1261 gccgggtcgg acctgatcat ggtcgacgac atctcggccg ggtgcgcggc ggtgctcgcc
     1321 gcccacgagg tcgcacgcac ccggatggta ctcgccggcc atccagagga tgacgtggtc
     1381 gcgttgtcga gtgagatact cgagtgcgtc ggtcccgctc gggtggcctc cacccttcgg
     1441 ggcgtgctcg tggcccgggg catccggttg cgctgccccg cgtgcaagct cccgtcaacg
     1501 gggggcccgg gatcgctcca gggaaagcag acgttcatcg ctcaaggctg cggggcgtgc
     1561 cacatgaccg gcttcggggg acagcgggtg ctggcccagg tctggtgcat gaaccaagag
     1621 gtccggcgct tgctccactc cggccgcatc cgggcggcag ttgagcggat cacgcaagag
     1681 acggcggcag gcctccgaga ccagggcctg gcgctggtcg aggacggcct caccccgccg
     1741 gacgaactgg ccgggatcgt ggatcaggtt tgatgaccat cacggatctc ctcatccaga
     1801 ccaaagagcg cggggcctcc gacctgcacc tgaaggccgg ggcccccccg tcgttccgca
     1861 tcaacggcaa actggtgcgg ccggatgcat ctccgctgag ccgcgaggag atgcacgcca
     1921 tgctgtacga cattctttcg gacgagcagc gggcccggtt cgaggcttcc cacgacctgg
     1981 acttcgcgat cgaactgaaa acgatcggcc ggttccgcgc gaacgcctat gtgcaccgat
     2041 tgggggaggg cctcgtcctc cgcctgatcc cctccaccat caagcccatt gaagagctcg
     2101 gcatgccccc catcctgagg gatctggcga tgcaggagcg cgggctggtg ctggtcacgg
     2161 gccccaccgg atcggggaaa tccaccacgc tcgccgccat ggtcgcgcac atgaacgcgc
     2221 accgggacga ccatatcatc accattgagg atcccatcga gttcatccac gaggacagga
     2281 aatgcagcat cagccagcgc gaagtggggc cccacacgga gtcgttctcg gccgcactcc
     2341 gcagcgcgct ccgcgaggat cccgacatca tcctggtcgg cgagatgcgc gatctggaga
     2401 cgatcggcca ggcgctcacc gccgcggaaa cgggccacct ggtgctgtcc acgctgcaca
     2461 cgaacagcgc cccgcagacc atcagccgcg tcgtggacgt ctttccgccg catcagcagg
     2521 agcagattcg cgtccaggtg gcggacgcac tgctcggagt gatcgcgcag acgctggtcc
     2581 cgaccctgga tggcacgggc cgcgtcgccg cgtgtgagat catggtggcc acgcccgccg
     2641 tccgcaactt gatccgggag aacaaggtcc accagcttcc ctcggccatt cagacggggg
     2701 tccgggaggg catgcaatcg ctcgatcaat gcctcaagaa cctcgtcaag acacggcgaa
     2761 tcaacaatga ggaggccgtg aagcgcgcca tcgagaaaca ggcattcctt cacgaggggc
     2821 ccggggtgcg agtcgaccac cccgaagcgc gcgtgcagcg atgatggcag atcgagtcac
     2881 gtggcgttga tcgttcgcgg acccatgggt caaagatcaa gtagacatca ggaccctgca
     2941 gcgatcacgg tgcggagcat cctggtcgtc gaagacgatc caagcgtcgt caagctcctg
     3001 cacctgtcgc tcgagaggga caggtgcagg gtcacgagcg ctgcggacgg ccgcgcggcc
     3061 cttgagatcg cgcagcgcga tcgtcccgac ctcgtgatcc tcgacctcgg gttgccagat
     3121 ctcgatggtc tggaggtgtg tcggcgtctg cgcgctacct ccgacgtgcc gattctcata
     3181 ttgacagggg cggccgagga ggtggacaag ctgctgggtt tgtccatggg tgccgacgac
     3241 tatgtgacca agccgttcag cccccgcgaa gtcgccgccc gcgtcaaggc catcctgaac
     3301 cgcaccaccc ggcccaggca ggagcgggtg ctgcgccgcc acgatctgga gctggacctg
     3361 gagcgccgcc gcgtcctgct ggccgggcag gaagtgagcg tgaccacgag tgagttcacg
     3421 ttgctgcgcg tgctcctgga gtcacctgag cgcgtgttct cccgtgacga actgctcacc
     3481 cagatctatc cccgcgatga ggcggcggtc gtggagcgtg ccatcgacgt ccacatcgcg
     3541 aacctacgaa ggaagcttgg cgacagtccc gccgatcctc gccacatcgc cactgtgcgc
     3601 ggcgcggggt acaaactcct gtaagaatca tcgcagcccc ccacaccgcc tccctctgct
     3661 cgccgggctg ccttgcttaa cttgaaaacg gcgcgatcat gatgcgggcc agctcgtact
     3721 ggtagggctg ctgaacatga taaccttgat accgtcggcg cggttgccct cgaccgcctc
     3781 gaaaatcggc tccgggatcc cgctcgcctc gtccagaatg cacagcaggt tcgcccccct
     3841 gatgccgaac atgcgctccg gcgaatccgt gctaaacccc acgatctcgc gaccgtcagg
     3901 catcagcaga cctgtgtcgg gatcggtgtg cagctcacca ccgttcggga ccctggcgtt
     3961 tgtgtgaagt gcccgctcgt gactgcagac cgtacaagtc ctccccatgg ccagcctccc
     4021 atgatcgtcg tgctagccag ggttagactg aaaagcgcac cacgaatccg gagaccctgt
     4081 ggagatcccc atgcgctcta aagccgacac gcgattcgta ttctattggc tatctaatac
     4141 tggaggtggt tcacgacttt cagtccaacc aggtactact ggaggctcga acaacgggt
//
LOCUS       DASQGH010000195         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_29275, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000195 DASQGH010000000
VERSION     DASQGH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29275"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1817
                     /locus_tag="VEW91_03705"
     CDS             156..1817
                     /locus_tag="VEW91_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYM90727.1"
                     /translation="MGRLIAFNDALNRPSSSAGVAAAIGQAALDLSDADGCVVYSHTP
                     EGNFACLWAKGVSPEGAPQALTTQFVQPAPDATGGAASLRLEFPGGHRVEGPRPTLVP
                     DILELPQDDAVRRRAEQEGYRALAVWPMASEGRVIAGVCCYYDEPRTWAQPEKEVFQT
                     FTWQAVSALKNAWLYEAQAERSFALEALYGLSSQLRAAGTFEEISSTLVEHAARLLHA
                     DHAVLSLLDADRQKFTCAHALGGAPEVKDSITPVARVILSRVAQTRMPHITEDIGREL
                     QPDPVAALRAAYCPVGPVIVVALQSEREVIGTIAVGRTKGKSARPFTQTEVRLLQGIA
                     EMGGTAISRSRLFHNLEEAYIQMVLALARAMDARDAYTGNHSERLAMQAEGVAQAMGI
                     PEEEVRDIRWAALLHDIGKIGSPDGILSKPGPLTESEWAIMQQHPAVGEGILLPVARM
                     QGVARIVRHHQEKWDGTGYPDGLRDEAIPLGARILAVVDAYGAITDERPYKIPSTHAE
                     AEAELKRCAGTQFDPKVVEVFCQMLARKGKEPALEFTGVSASIDR"
     gene            complement(1823..2137)
                     /locus_tag="VEW91_03710"
     CDS             complement(1823..2137)
                     /locus_tag="VEW91_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HYM90728.1"
                     /translation="MASVKVARTEEVPIGKGKVVKAAGRALALFNIGGKFYAVDNRCT
                     HVGGPLGEGAVAGNVVTCPWHGSRFDVTTGQVIEPPARRPVRTFPVKVVEGAIYVEVP
                     GS"
     gene            complement(2186..3235)
                     /locus_tag="VEW91_03715"
     CDS             complement(2186..3235)
                     /locus_tag="VEW91_03715"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYM90729.1"
                     /translation="MREGGYFSDADRELTQAIGLINSHDDFPLVIAKRRSDGIYDSLR
                     TYWLPRFRAGGIRAVVGAVYTPSIYLPEAALRHAMRVIDALYEELGDNQDDVVLARSA
                     ADIQRINNAGRIAVLLAVEGAEPLGHDLGTLRLFHRLGLRMLSFTWHRRTAFGDGAWE
                     NGSRGGLTRLGHQAVKEMNRLGIIIDVSHASDQTTWDILEASTKPVIASHSNARALRD
                     HPRNLTDEMIRAIASSDGIVAVTAVARFITEGTPTIGDWADHLAHIIRVADIDHVGIG
                     ADFADYLIEIGASPEIVDWSPDIGVRRTKFEGMLSPEDLPGLTRELVRRGFAERELRK
                     IYHENYLRVFSSILG"
     gene            complement(3325..4200)
                     /locus_tag="VEW91_03720"
     CDS             complement(3325..4200)
                     /locus_tag="VEW91_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90730.1"
                     /translation="MSAVLATHPPRRAAALSWRRLVRSPNLVVGAGIMALVIGSAVFA
                     PQLAPYSPIDQAFTDQLRPPSPAHLFGTDEFGRDVFSRALYGARIALVIGVLADGIAA
                     VLGVLLGVLSGYFGGKIDAVVMRSVDVMLAFPYLLLAMVVVTILGPSLTNAMIAIGIV
                     YAPQFARLVRGAVLAIKEQEFVEAAGAVGAGVVRILGRHILPNILSPIIVMATLTVGF
                     TIVETAGLSFLGLGASPPTPEWGSMLATGRSFMLTAPWIATFPGLAIMVTVVGFNLIG
                     DGLRDLLDPRLRGGG"
     gene            complement(4203..>5273)
                     /locus_tag="VEW91_03725"
     CDS             complement(4203..>5273)
                     /locus_tag="VEW91_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90731.1"
                     /translation="APRHLGRPPARGARAGSGDERLMLKYIARRLGQLVPVLLGVSIL
                     VFFGMHLVPGDVAQLLLGEKGTAADLQRIRHQLGLDQPVYVQYVRFLAGVLVGDFGVS
                     LRTRQSAIWEIGQGLPVTIELSLAALLYAVVFGLAIGVLAARRPHSLLDTTAMVGVLL
                     GVSMPVFWTGILLLLVFGGILGWLPLGGILDGGMTFHRITGMSLVDGLLTGNGAVVQS
                     TLRHLILPSVALGATAMATIARMARSTMLEVLHQDYVRTARAKGVVERRVVSHHALRN
                     ALLPIVTLVGLQLGLLLSGAVLTETIFALPGLGRLAIISVLARDYPVVQGVVMIAAAI
                     FVLANLLVDILYAYLDPRIRYD"
BASE COUNT          995 a         1754 c         1716 g          808 t
ORIGIN      
        1 ggggacggtg cgtcggtgcg cttgcagtgg cctccgccta cccacggcct tggacaggag
       61 cggagatcgt cctggccgag gcggttgcac agcaagtatc ggcaactgct gaggggctgc
      121 gcctattcca ggagacccaa cagcgcgcca gattgatggg gcgcctcatc gcatttaacg
      181 atgcgctgaa ccgtccgtcc tcatcggcag gtgtggcggc ggcaatcggg caggcagccc
      241 tcgacctcag cgacgccgac gggtgtgtgg tgtactcaca cacgccggag ggaaactttg
      301 cctgtctctg ggcgaaaggg gtctctcctg aaggcgcccc tcaggccctg acgactcagt
      361 tcgtccaacc ggcccccgat gcgaccggag gagcggcatc tctccggctc gagttccccg
      421 ggggccacag ggtggaggga cccaggccca ccctggttcc cgatatccta gaactacccc
      481 aggacgatgc ggtccgccgc cgggcagagc aggaaggcta ccgggctctg gcagtgtggc
      541 ccatggcctc cgagggacgt gtgatcgccg gggtgtgttg ctactatgat gaaccccgca
      601 cctgggcgca gcccgaaaag gaggtcttcc aaaccttcac ctggcaggca gtctccgcac
      661 tcaagaacgc ctggctgtat gaggcgcagg ccgagcgcag cttcgcgctg gaggccctct
      721 atggtctcag cagccagctg cgggcggccg gcacgtttga agagatatcc tcaacgcttg
      781 tggagcatgc tgcgcgcctg ctccacgccg accatgcggt gctctcactc ctggacgccg
      841 atcgtcaaaa gttcacctgc gcccacgccc tcgggggcgc gccagaggtg aaggattcga
      901 tcacgcccgt tgcgcgcgtg atcctaagtc gggtggccca gacgcgcatg cctcatatca
      961 ccgaggacat cgggcgggag ctgcaacccg atccggttgc cgcgctccgg gcagcgtact
     1021 gcccggtggg tcccgtgatc gtggtggcgt tgcaatccga acgagaggtc atcggtacca
     1081 ttgccgtggg gcggacgaaa ggaaagagcg cccgcccatt cacccagacc gaggtacggc
     1141 tgctccaggg catcgccgag atgggcggaa cggccatcag ccggtcccgt ttgttccaca
     1201 atcttgagga agcgtacatc cagatggtcc tggccctggc gcgcgcgatg gacgcccggg
     1261 acgcgtacac ggggaaccac agcgagcgcc tggccatgca ggccgaagga gtggcgcagg
     1321 cgatgggcat tcccgaagag gaggtcaggg atattcggtg ggcggccctc ctgcacgata
     1381 tcggcaagat cggctcgccc gatggcatcc tctcaaagcc cgggcctttg acggagagtg
     1441 agtgggcgat catgcaacag cacccggccg ttggagaggg gatcctgctt cctgtcgccc
     1501 ggatgcaggg ggtggctaga atcgtccgcc atcatcaaga gaaatgggac gggaccgggt
     1561 acccagacgg cctccgtgac gaagcgatcc cgcttggggc ccgcatccta gcggttgtcg
     1621 acgcctacgg cgcaatcacc gatgagcggc catacaagat cccaagcacc cacgcagagg
     1681 ccgaggccga gctcaagcgc tgtgcaggca cacagttcga tccgaaggtt gtcgaggtct
     1741 tctgtcagat gcttgcgcgc aaaggaaaag agcccgccct cgaattcaca ggagtctcag
     1801 cgagcatcga ccgctgacgc cattaggacc cgggcacctc gacatatatc gcgccctcca
     1861 cgaccttcac cgggaaggtc cttacaggcc ggcgagccgg cggttcgatc acctgccctg
     1921 tcgtgacatc gaatcgactc ccgtgccagg gacaggtcac cacattcccc gccacagctc
     1981 cttcgccgag cggtcccccc acatgcgtgc atcggttgtc gacggcgtag aatttgcccc
     2041 cgatgttgaa gagcgcgagg gccctccccg ccgcctttac gaccttgcct ttcccaattg
     2101 gaacctcttc agtccgcgcc acctttaccg aggccattag cttcccctcc ctccaggctc
     2161 cggcaatcgc gtcgacagat accggctaac caaggatgct cgagaagaca cggaggtagt
     2221 tctcatggta gatcttgcgc aactcgcgtt cggcgaatcc ccggcggacc agctcccgcg
     2281 tcaagcccgg gagatcctca gggctcagca tgccttcaaa cttggtgcgc cggacgccaa
     2341 tatctgggct ccagtcgacg atttctggcg atgccccgat ctcgatcagg taatcggcaa
     2401 aatcggcacc tatgccgacg tggtcgatat ccgcaacgcg aatgatgtgc gcgaggtggt
     2461 ccgcccagtc gccgatggtg ggcgtgccct ccgtaatgaa tcgcgccaca gctgtcacgg
     2521 cgacgatacc atcggaggag gcaatcgcgc ggatcatctc atcggtcaag ttgcgcgggt
     2581 gatcgcgcag ggcacgagcg ttcgaatggc tggcgatcac cggcttcgtt gacgcctcaa
     2641 ggatgtccca ggtggtctgg tcggatgcgt gtgacacatc gatgataata cccagccggt
     2701 tcatctcctt cacggcttgg tggcccaggc gcgtcaggcc gccccgtgag ccattctccc
     2761 aggcgccgtc gccaaaggcg gttcgtctgt gccaggtaaa cgagagcatc cggagtccaa
     2821 gcctatgaaa caggcgtaat gtgcccaggt cgtgtcccag gggctccgcg ccctcaaccg
     2881 caagcaacac ggcaattcgc ccggcgttgt tgatgcgctg aatgtccgcg gccgaccgtg
     2941 ccagcactac atcgtcttga ttgtcgccca gttcctcata cagggcatcg atgacccgca
     3001 tcgcatgcct cagggcagct tcgggcagat agatggaggg tgtatagacg gctcccacaa
     3061 cggcccgaat cccgccggcc cgaaaccgcg gcagccaata cgtccgaagc gagtcgtaga
     3121 tcccgtccga ccgccgcttg gcgatgacca gagggaaatc atcgtgcgag ttgatcagtc
     3181 cgattgcctg ggtcagctca cggtccgcgt ccgaaaaata tccgccctcg cgcattgatc
     3241 acccctgctc ccgacgagaa tgccctgcac acccgtgcaa gcgtcaatgc gtatgcaaca
     3301 ctgcacccac cgatacctcc tcccctaccc gccgccccgg aggcgggggt cgagcaggtc
     3361 ccggagtccg tccccaatca ggttgaaccc gaccacggtg accatgatgg ccaggcccgg
     3421 aaacgtggcg atccagggag ccgtcagcat gaacgaccgg ccggtggcga gcatcgaccc
     3481 ccactccggg gtcgggggag aggcacccag gccgaggaac gagagtccgg cggtctcgac
     3541 gatcgtgaac cccacggtca gcgtggccat cacgatgatg ggggagagga tgttcggcag
     3601 gatgtggcgg cccaggatcc gcacgacgcc ggccccgacc gcccctgccg cctcgacgaa
     3661 ctcctgctcc ttgattgcga gcaccgctcc ccgaaccagc cgggcaaact gcggcgcgta
     3721 gacgatcccg atcgcgatca tcgcgttggt gaggctcggg cccaggatcg tcaccacgac
     3781 catcgcgagg aggaggtacg ggaaggccag catgacgtcg accgaccgca tcacgaccgc
     3841 gtcgatcttc ccgccgaagt acccagatag cacgccgagc agcacgccga gcaccgcggc
     3901 gatgccgtcg gccagcacgc cgatcaccag ggcgatgcga gcgccgtaga gggcgcggga
     3961 gaagacatcc cggccgaact catcggtgcc gaatagatgc gccggggacg gcggccggag
     4021 ctggtcggtg aacgcctggt cgattgggct gtagggcgcg agttgcggcg cgaagaccgc
     4081 cgagccgatc acaagcgcca tgatcccggc cccgaccacc aggttggggc tccgcaccag
     4141 gcggcgccag gacagcgcag cggcgcggcg gggtgggtgt gtcgccagaa cggcgctcat
     4201 cgtcaatcgt agcggatccg cggatccagg taggcgtaga ggatgtcgac aaggaggttg
     4261 gccaggacaa agatcgcagc cgcgatcatg acgaccccct gcacgacggg gtagtcccgg
     4321 gccagcacgc tgatgatcgc cagccggccg agccccggca aggcgaagat cgtctcggtg
     4381 agaaccgctc cgctgagcag caaccccagc tggaggccga ccagggtgac gatgggcaac
     4441 agggcgttgc ggagcgcgtg gtggctcacc acccggcgtt cgaccacccc cttggcccgg
     4501 gcggtgcgca cgtagtcctg gtggagcacc tccagcatcg tcgaccgcgc catgcgggcg
     4561 atggtggcca tcgcggtggc gccgagcgcg accgagggga ggatcaggtg gcggagggtg
     4621 ctctggacca cggccccgtt gcccgtgagg aggccatcca cgagcgacat gcccgtgatg
     4681 cgatggaacg tcatcccccc gtccaggatg cccccgaggg ggagccagcc caggatcccg
     4741 ccgaacacca ggagcagcag gatcccggtc cagaacaccg gcatcgatac gccgagcaga
     4801 accccgacca tcgccgtggt atccaggagc gagtggggcc ggcgggccgc gaggacgccg
     4861 atggccaagc cgaagacgac cgcgtacagc agggcggcca ggctcaactc gatcgtcacc
     4921 ggcagcccct gcccgatctc ccagatcgcc gactgacggg ttctgagcga aacgccgaag
     4981 tcgccgacga gcacccccgc caggaaccga acgtactgca cgtacaccgg ctggtcgagc
     5041 ccgagttggt ggcggatgcg ctggaggtcg gccgcggtcc ccttttcgcc gagcaggagc
     5101 tgggcgacgt cgcccgggac gaggtgcatc ccgaagaata caaggatgct cacccccagc
     5161 agcacgggga cgagctgccc cagccgccgg gcgatgtact tgagcatcag gcgttcatcc
     5221 ccactgcctg cccgtgcccc ccgcgccggc ggccggccca ggtgccgggg ggc
//
LOCUS       DASQGH010000196         4697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51728, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000196 DASQGH010000000
VERSION     DASQGH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51728"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..574
                     /locus_tag="VEW91_03730"
     CDS             179..574
                     /locus_tag="VEW91_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90732.1"
                     /translation="MSPGLTVVVAGDDLLFSTRVASALGRCGHEVRVVHTAAEFQGAL
                     REGPDAGIVNLASFRLDAIEAIRQAKLDPGTRTIPLLGFCGHADTPRQAAARAAGCDL
                     IAANAEVLANLPRLLTALLDTPRSSASRR"
     gene            complement(670..1911)
                     /locus_tag="VEW91_03735"
     CDS             complement(670..1911)
                     /locus_tag="VEW91_03735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYM90733.1"
                     /translation="MVSEAEVVIIGSGAFGSSTAYHLAALGHKGVVLVDAHEIASQTS
                     PRAAGLTKQIRPHQEMSRLAIRSVEKIKRFAEELGEPLTYVQSGSVSIARGEHDAAVI
                     RAEIAAGKALDLETYEISFDEMSLLAPLIRSTDIRLIAYTPFDLYLEEPGQLPLGYAR
                     AAEKQGVTLLPNTRVTGIKVRDRRVSAVVTKQGEIRTRVVVDAAGAWSRLVAEQVRLR
                     IPLIPVRHQLYITEPIPGVEAGHAICRIYDAGVYLRPHQGGLLVGGYERDPQFHDMHK
                     FPPEFQIGDLPLELSVLERLTESVRAQVQLDDQLPIREHRGGLPTMTADGLPLIGPVA
                     AIGGFFIVSGCCVGGLSISPAVGEMLAEWVHTDQPSPILKTFSPSRFGREYDSDAGLR
                     EACLRQYAGWYLAMPQVPGSR"
     gene            2114..2761
                     /locus_tag="VEW91_03740"
     CDS             2114..2761
                     /locus_tag="VEW91_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90734.1"
                     /translation="MATPRLPTLLPQPRNGLVLLTGHESVLPLGQLLCLQYLGRGDPV
                     VLVDGANAFNPYLLADAARAHGLDLRRVLDAVHLSRVYTCHQLEALWAERLRPAIASF
                     RPRAILCLGLLDPLYDEDVPAAEAARIFRRLAPAIRDAAAHLPILAACPDPAVSDRGA
                     DDHRWRFGDGLRGMASWHFTAQPENGRVWISRERPDPARWEWTLFSPDRTFRRRT"
     gene            2777..3058
                     /locus_tag="VEW91_03745"
     CDS             2777..3058
                     /locus_tag="VEW91_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90735.1"
                     /translation="MGRTVPTMTQMVQSEEETWAPFRRALRAQDRAAFDRLFAAARHY
                     AAVASYAARPVPFEAILLSMLLEAMKAIQQLEAEVTAFRATLPAPRPPV"
     gene            3024..>4697
                     /locus_tag="VEW91_03750"
     CDS             3024..>4697
                     /locus_tag="VEW91_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HYM90736.1"
                     /translation="MPPSLLHAHLFDTYPTSGGMVVWLLRDGARLRLVHPWTPRLYYG
                     GTRRAREQAEALLHGIRVPTTTRRTTRRDLLQGEIEVVEVTVADPMAFSRVAARLAGI
                     VGLTLYNCDIALPQMYLYEHRLFPLGRCAVEATPEGTIRRIAPLESPWEREYIAPPLV
                     VLRLRCDGDPANPNHGRPAALHVAVDGEASTLTGDTPADLLEALDRWIRRYDPDVILT
                     EWGDSFLFPTLRRLAAACGRPLPLNRDGGAGIRTRRPRSYVTYGQIVYTAGGSYLRGR
                     WHLDTANSFAYEESELPGLLELARLSRLPVQHMARTSVGTAITSMQLDQAYQDGILIP
                     WRKSRPEAFKSGSELLLTDRGGLTYTPLVGAFEQVGELDFAAMYPAMMSRFNISQETV
                     NCACCREDPAARVPGIPHHLCQKRRGLIPQVLERVLERRAYYKARRAATTGTERHLYD
                     MRQTALKWIGVVCLDGDTLVLHKAENRWRIASIREIVDAHLPADETGIKPVDGLAVIG
                     VDNHLLPRIRFVRHAIKMPAPQTMIRVRLKWNRELLMTPDHPCYVLEDGH"
BASE COUNT          719 a         1716 c         1540 g          722 t
ORIGIN      
        1 acggcggtcg gatgcgcgct cgggccgcaa ctcgtcagcg aggtcgaaac caaactgctg
       61 agcgtggatg gcgtcgagga ctgcaaggtc gagatggtct ggagcccgcc gtggagcccg
      121 gagcggctga gcgacgacgg ccggctctcg ctccaggcca tgggctaccc cctgtgagat
      181 gagccccggg ctgaccgtcg tcgtggccgg cgacgacctg ctgttctcca cgcgggtcgc
      241 gtcggcgctg gggagatgcg gccatgaggt gcgggtcgtc cacaccgccg cggagttcca
      301 gggcgccctc cgcgagggac cggacgccgg catcgtcaac ctcgcctcgt tcaggctgga
      361 cgccatcgag gcgatccggc aggccaagct cgaccccggc acccggacga ttcccctgct
      421 tgggttctgc ggacacgccg acacgccgcg ccaggcggcg gcccgggccg ccggatgcga
      481 tctcatcgcg gccaacgccg aggtcctggc caacctcccc cgcctcctca ccgcgctgct
      541 cgacaccccg cgctcatcag cgagccgccg ctagtctagc ggcgagttcg ggtcgcggcc
      601 cggcgcctcc ttcccaggcg gatccgcccg ccgggggagc cgtcgaagga gcgcacgcac
      661 ggggcgctgc taccgagacc cgggcacctg gggcatggcc aggtaccacc cggcatactg
      721 tcgcaaacac gcctctcgca gcccggcgtc cgagtcgtac tcccgcccga accgggaggg
      781 ggagaacgtc ttgaggatgg gcgacggctg atccgtgtgg acccactcgg ccagcatctc
      841 ccccacggcg ggggaaatcg agaggccgcc gacgcagcac ccgctgacga tgaagaaccc
      901 cccgatcgcc gcaaccgggc cgatgagcgg gagcccgtcc gccgtcatcg tcggcagccc
      961 gccccggtgc tcccgaatcg ggagctggtc gtcgagctgc acctgcgcgc gaaccgactc
     1021 cgtcaggcgc tccagcacac tcaactcgag cggcaggtcc ccgatctgaa actcgggcgg
     1081 aaacttgtgc atgtcgtgga actgcgggtc gcgctcgtag ccccccacca acagcccccc
     1141 ctgatgcggt cgcaggtaca caccggcgtc gtagatccgg cagatcgcat gccccgcctc
     1201 gaccccgggg atcggctcgg tgatgtagag ctggtggcgc acgggaatga gggggatgcg
     1261 gagccggacc tgctccgcca ccagcctcga ccaggccccc gccgcgtcga cgaccacgcg
     1321 tgtccggatc tccccctgct tcgtgaccac cgcggacacg cgtctgtccc ggaccttgat
     1381 ccccgtcacc cgggtgttgg gcaggagcgt cacgccctgc ttctcggccg cccgggcgta
     1441 tcccagcggt aactggccgg gctcctcgag gtagagatcg aagggcgtgt aggcgatgag
     1501 gcggatgtcc gtggatcgga tgagcggggc gagcaaggac atctcgtcga aggagatctc
     1561 gtacgtctcc aggtccaacg ccttgccggc ggcgatctcc gcccggatca cggccgcgtc
     1621 gtgttctccc cgcgcgatgc tgacgctgcc cgactgcacg tatgtcaggg gctcccccag
     1681 ttcctcggcg aagcgcttga tcttctcgac gctgcggatc gcaagacgcg acatttcttg
     1741 gtgcggccgg atctgcttcg tcagtccggc ggcccgcggc gacgtctggg aagcgatctc
     1801 gtgggcgtcc acgaggacga cacccttgtg gccgagcgcc gccagatggt aggcggtgct
     1861 cgagccgaac gcgcccgacc cgatgatcac gacctcggct tcgcttacca tcccccctag
     1921 gttcaacatg taagggcgcg tcccccccaa accacggcgc cgcgcggctc gggcaggcaa
     1981 agcgggctaa tccctgcata ttccctatct ggccgcgatg gatcgccttt ctgagcgcac
     2041 cagcccccta gggcgccgtt gatgcaaaca tatgttcggt attatactgg ggatacccga
     2101 aaggcggtga ccgatggcca cgccccgtct gcccaccctc ctcccccagc cccgcaacgg
     2161 gctcgtcctg ctcaccggcc acgaaagcgt cctgccgctc ggccagctcc tgtgcctcca
     2221 gtacctcggc cgcggcgatc ccgtggtgct ggtggacggg gcgaacgcct tcaatcccta
     2281 cctccttgcc gacgccgcgc gggcgcatgg gctcgacctc cgccgcgtgc tggacgccgt
     2341 ccacctctcc cgggtgtata cctgccacca gttggaagcg ctgtgggcgg agcgcctccg
     2401 gccggccatc gcgtcgttcc gcccccgcgc catcctctgc ctcggcctgc tcgaccccct
     2461 gtacgatgag gatgtcccgg ccgcggaggc cgcccgcatc ttccggcgtc tcgcgccggc
     2521 gatccgcgat gcggcggccc atctcccgat cctggccgcc tgccccgatc ctgcggtatc
     2581 cgatcgcggc gcggacgatc accggtggag gttcggcgac gggctgcgcg ggatggccag
     2641 ctggcacttt accgcccagc cggagaacgg ccgcgtgtgg atctcccggg agcggcccga
     2701 cccagcccgg tgggagtgga cgctgttctc tccggaccgc accttccgaa ggcggacata
     2761 gacgaggagg ccggtgatgg gccgcacggt gcccacgatg acccagatgg tccagagtga
     2821 agaagaaacc tgggctccgt tccgccgcgc gctccgcgcg caggaccggg ccgcgttcga
     2881 ccggctcttt gccgccgcgc gccactacgc cgccgtcgcc agttacgctg cccggccggt
     2941 gccgttcgag gcgatcctcc tgtcgatgct gctggaagcg atgaaggcga tccaacagct
     3001 cgaagccgag gtcacagcgt ttcgtgccac cctccctgct ccacgcccac ctgtttgaca
     3061 cctatcccac gtcgggcggg atggtcgtct ggctcctccg cgacggcgcc cggctgcggc
     3121 tcgtgcaccc gtggacaccc cgcctgtact acggcgggac ccgccgcgcc cgcgagcagg
     3181 ctgaggcgct gctccacggc atccgggtac caacgaccac ccggcgcacg acgcgtcgcg
     3241 acctgctgca aggagagatc gaggtcgtgg aagtcacagt cgccgatccg atggcgttct
     3301 cccgcgtcgc cgcccggctc gcggggatcg tcggcttgac attgtacaac tgcgacatcg
     3361 cgctaccgca gatgtacctg tacgaacacc ggctcttccc gctcgggcgg tgcgcggtcg
     3421 aggcgacgcc tgagggcacg atccgccgga tcgcgccgct cgagtccccc tgggaacggg
     3481 agtacatcgc gccgccgctc gtggtcctcc gcctgcgatg cgacggcgat cccgccaacc
     3541 cgaaccacgg gcgcccggcg gccctccacg tcgccgtgga tggtgaagcg agcaccctca
     3601 cgggggacac tcccgcggac ctcctggagg cgctcgaccg atggatccgc cggtacgatc
     3661 cggatgtcat tctcaccgag tggggcgact cgttcctctt cccgacgctg cggcgccttg
     3721 cggccgcgtg tggccgcccc cttccgctga accgcgatgg aggagcgggg atacggaccc
     3781 gccgcccgcg ctcgtacgtg acgtacggcc agatcgtgta caccgcgggc ggatcctacc
     3841 tgcggggccg ctggcacctc gacaccgcca actcctttgc ctacgaagaa tcggagctgc
     3901 ccgggctgct ggagctggcg cggctgagcc ggctgcccgt gcagcacatg gcccggacga
     3961 gcgtcgggac cgcgatcacc tcgatgcagc tcgaccaggc ctatcaggac ggcatcctga
     4021 tcccctggcg gaagtcgcgc cccgaagcgt tcaagtcggg gagcgagctg ctcctcaccg
     4081 accggggcgg gctcacgtac acgccgctcg tcggtgcgtt cgagcaggtc ggcgagctgg
     4141 acttcgcggc gatgtacccg gcgatgatga gccgcttcaa catctcccag gaaaccgtca
     4201 actgtgcctg ctgccgggag gacccggccg cccgggttcc gggcatcccg caccacctct
     4261 gccagaagcg gcgggggctc atcccgcagg tcctggagcg cgtgctcgag cgccgcgcct
     4321 actacaaagc ccgccgggcg gcgacgaccg ggacggagcg ccatctgtac gatatgcgcc
     4381 agacggcgct caaatggata ggggtcgtct gtttggacgg cgacacgctc gtcctccaca
     4441 aagcggagaa ccggtggcgc atcgcttcga tccgtgagat cgtggacgcc caccttccgg
     4501 ctgatgagac cggcatcaaa ccggtcgacg gcctggccgt tatcggagtc gacaaccacc
     4561 tgctcccccg catcaggttt gtgcgccacg cgatcaaaat gccagctccc cagacaatga
     4621 tccgggtcag gctgaaatgg aaccgggaac tgctgatgac ccctgatcat ccatgttacg
     4681 tgctggagga cggtcat
//
LOCUS       DASQGH010000197         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46213, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000197 DASQGH010000000
VERSION     DASQGH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46213"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VEW91_03755"
     CDS             <1..333
                     /locus_tag="VEW91_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM90737.1"
                     /translation="SVPRLYEHAILTLGASRWRMLRDVLLPGAFPGIVTGIRLGLGFG
                     WRALIAAELVAVTQGLGFMIYSAANYLRTDIILAGILVIGLVAITMDSLILVPIERAT
                     VLRWGMYQ"
     gene            330..770
                     /locus_tag="VEW91_03760"
     CDS             330..770
                     /locus_tag="VEW91_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90738.1"
                     /translation="MSGGGTGAGSSSARPPRRWFLHRTVLFVGILAVVGTAWSAYTGA
                     TARQKMDPALLAALTARQPVNIWVELPFPPEEFHIRYLQDRGTVTGVRGRWIHLMRVR
                     PGVAWSIARLYWVQRVRGEPGPQGAAESLDRLPQRRADHAVLSV"
     gene            751..1125
                     /locus_tag="VEW91_03765"
     CDS             751..1125
                     /locus_tag="VEW91_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM90739.1"
                     /translation="MPFYRFEQLPQETISPDYSTAAGGTVTGEVIEVGKYAMAKGTGA
                     DPHRHPNEQIIYVLRGKLRARVGQDERILEPGDVVHIPPDVVHEIRAMESSEFLSSKN
                     LVGGKGSRGWSAAAPVRPRPTR"
     gene            1160..1495
                     /locus_tag="VEW91_03770"
     CDS             1160..1495
                     /locus_tag="VEW91_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM90740.1"
                     /translation="MPFFRFADIPSEYVTPKYSKAFGPLLTGTQIEVGRLHFGKGEGA
                     VPHAHPQEQIMVVLTGRLRAVLDGQTTDLGPGEGFLAPPNVQHQVTALEDTEALSCKG
                     LVDGRGHRI"
BASE COUNT          235 a          498 c          540 g          239 t
ORIGIN      
        1 agcgtgcccc gcctgtacga gcacgcgatc ctcaccctgg gcgcgtcacg gtggcggatg
       61 ttgcgggacg tcctgctccc cggggccttc cccggcatcg tcaccgggat ccgcctgggg
      121 ctggggttcg gctggcgggc gttgatcgcg gccgagttgg tcgcggtgac ccagggcctg
      181 ggattcatga tctacagcgc cgccaactac ctccggaccg acatcatcct ggcgggtatc
      241 ctcgtcatcg gacttgtcgc gatcaccatg gacagcctga tcctcgtgcc gatcgagcgc
      301 gccaccgtcc tccggtgggg gatgtaccag tgagcggcgg ggggaccggc gccgggtcgt
      361 cctccgcgcg tcctccgcgg cggtggttcc tgcaccgcac cgtgctcttc gtggggatcc
      421 tcgccgtggt ggggacggcc tggtccgcgt ataccggcgc caccgcccgc cagaagatgg
      481 acccggcgtt actcgccgcc ctcaccgcac gccagccggt gaacatctgg gtcgagctcc
      541 cgttcccgcc cgaggaattc cacatccggt atttacaaga taggggcacc gtgaccggtg
      601 tgcgcgggcg ctggatccac ctcatgcggg tgcggccggg cgtggcctgg tcgatcgcgc
      661 ggctgtactg ggtgcagcgc gttcggggcg agcccggacc acaaggggcc gcggagtccc
      721 tggacagatt gccacaaagg agggcagacc atgccgtttt atcggtttga gcaactcccg
      781 caggaaacga tctctccgga ctactcgacg gccgcaggcg ggacggtcac gggcgaggtc
      841 atcgaggtcg ggaaatacgc gatggcgaag gggaccgggg cggaccctca ccgccatccc
      901 aacgagcaga tcatctacgt gctccggggg aagctccggg cccgggtggg ccaggacgag
      961 cggatcctcg agccggggga tgtcgtccac atcccccccg atgtcgtcca tgagatccgc
     1021 gcaatggagt ccagcgagtt cttgagcagc aagaatctgg tcggcggcaa ggggagccgg
     1081 ggctggtcgg cggcagcgcc cgtccgacct cggcccacgc gctgatcggc ccgcggccgg
     1141 acgaggggga ggaggatcga tgccgttttt ccggttcgcc gacatcccga gcgagtacgt
     1201 gacgccgaaa tactcgaagg cgttcggtcc gctcctcacc gggacgcaga tcgaggtcgg
     1261 acggctgcac ttcgggaagg gggaaggcgc ggtgccccat gcgcaccccc aggaacagat
     1321 catggtcgtg ctgacggggc gtctccgggc cgtcctcgac ggccaaacca ccgacctggg
     1381 cccgggcgaa gggtttctgg cgcctccgaa cgtgcagcat caggtcacgg cactcgagga
     1441 cacggaggcg ttgagctgca aggggctcgt ggacggccgc gggcatcgca tctgacgcgc
     1501 aggatgcgct tc
//
LOCUS       DASQGH010000198         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54623, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000198 DASQGH010000000
VERSION     DASQGH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54623"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..1117
                     /locus_tag="VEW91_03775"
     CDS             440..1117
                     /locus_tag="VEW91_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HYM90741.1"
                     /translation="MNVRLFASYREAVGAPRVEVALQRGSRGEAVWAALVSRYPALAR
                     LPEPAGYAINDEYVDGARPLEDDDEVALIPPVSGGGESRVTQRAPLIEMTDGPIPIDR
                     LLGAVADPRAGAVVLFVGIVRDNARGRRVDHLVYEAYEALARREMEKIAAMITSRWPV
                     TRVAMIHRTGHLEVGEASVAVSVSAPHRAEAFEAARFAIDTLKMTVPIWKKEIWEGGE
                     AWVGTEP"
     gene            1101..2651
                     /locus_tag="VEW91_03780"
     CDS             1101..2651
                     /locus_tag="VEW91_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYM90742.1"
                     /translation="MAPSPEPLRATRIIGDPIRHLSLAVGAAIGLYSGALLWDAHQGW
                     NALAAVLAGGVGGLGLEYEGSPLVPYAGYVFGLLSITLAASATGLGVLAYGPWIALGG
                     ALFPTWPAFAGLAAVDVLSVGFALTEGRSPSPLTLLAPAAAVICLHLGAVLVSREVRA
                     RSHLALTDPLTGLANRRLLGWRLGEELSHARRTGGTFALMYLDLQGFREVNTRHGHRA
                     GDRALVQVAQILRDTMRAHDVIARTSGDEFAILAPGLGEPEAVVVVERIRAAISRATT
                     LPLPLRLAAGWAIAPRDGMEAGDLLETAASSVFEQKLASRSAGPSLPTELMTALWSLP
                     EGAQQLVRLLHTEGIELEEHLSHVGQWSMDLGRMVGLDPARQTALAQAVLVHDVGKLV
                     VPVALLRKPGPLTRDEEAIVAGHVTSGAALLRATGVDEAVVSIVAAHHERWDGGGYPA
                     GLAADQIPLEARILAIADGYDTMVARRPYRKAWTTTAAIADVQLEGGHQFDPELVTLI
                     IPVISASG"
     gene            complement(2665..>3675)
                     /locus_tag="VEW91_03785"
     CDS             complement(2665..>3675)
                     /locus_tag="VEW91_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYM90743.1"
                     /translation="HRHGGPEELIYEEAPTPGLGARDVLVRVRACAMNHLDIHVRRGI
                     PGMTVRFPHILGCDVAGVVERVGAEVTAWRPGDEAAIAPGLSCGRCQACLAGNDNLCR
                     EYAILGEHTAGGYAEFVRVPEANVLAKPAGLSFPEAAAVLLVFLTAWDMLVVGARVQP
                     EETVLVWGAGSGVGSAAIQIAKVFGARVLAVAGADWKLERARRLGADETINHATQRVG
                     DEVRRLTNRRGVDVVFEHVGEATWDTSLKALARGGRLVTCGATSGPAAETDLRYVFSR
                     KLLIRGTFMGSRATLHDIFRLISAGRLRPVVHEVFPLRDVRRAHELMEQSAHFGKLIL
                     VP"
BASE COUNT          518 a         1291 c         1316 g          550 t
ORIGIN      
        1 cgaagcgagg gatgccggag gtcacgcagg agaaacacta atcgccggtc acgctcgctg
       61 gatgcgcggt ggtgccccta ggtaggcctg acggaatcgc tccgtgtagg caagcttcac
      121 gaagcgggcg agcgcttgcg gcgtcggaga gagcgcactg cttccctcgc cgatcggaac
      181 ggtccgtaga cccggccttt cctgacatcc tctagggcaa gcgcgagctc gcggtcaacg
      241 atccgttttg gggtgagggt aatcttcgtg tccttcacct gggcttcaag cagatcacca
      301 acagcgatac ccgccttccg ccggaccgtg ggggtgcttg acttcagccc tgaaagtaag
      361 cgcttccagc tgctcgcccg tgcgcccggc cccgccctcg ggacgcggcg ccgcctatgg
      421 tacaatcggg tttgtgaagg tgaacgtccg gctcttcgca tcgtaccgag aggctgtggg
      481 cgctccccgg gtcgaggtcg cgctgcaacg cgggagccgc ggggaggcgg tgtgggcggc
      541 gctcgtctcc cgctatccgg ccctcgcgcg gctgcccgag ccggcgggat acgcgatcaa
      601 cgacgagtac gtcgatggcg cgagacccct cgaggacgat gacgaggtgg cgttgatccc
      661 tccggtgagc ggcggagggg aaagccgcgt cacgcagcgc gccccgctga tcgagatgac
      721 cgacggcccg atcccgatcg accgtctcct cggagcggtc gccgatcccc gggccggcgc
      781 cgtggtcctc ttcgtcggga tcgtccgcga caacgcgcgc ggccggcgcg tcgaccacct
      841 ggtgtacgag gcgtacgagg cgctcgcgcg ccgggagatg gagaagatcg cggcgatgat
      901 cacctcccgg tggccggtga cccgggtcgc catgattcac cggaccggcc atctcgaggt
      961 aggggaggcg agcgtggcgg tcagcgtctc ggccccgcat cgggctgagg cgttcgaggc
     1021 ggcccgcttc gcgatcgata ccctcaagat gaccgtcccc atctggaaga aggagatctg
     1081 ggagggcggg gaggcctggg ttggcaccga gccctgagcc cctgcgcgcc acccggatca
     1141 tcggggatcc gatccggcac ctctccctgg ccgtcggcgc agcgatcggg ctgtactcgg
     1201 gagcgctcct gtgggacgcg caccaggggt ggaacgcgct cgcggccgtg ctggccgggg
     1261 gggtgggcgg gctggggctg gagtacgagg ggtctccgct cgtgccgtat gcggggtacg
     1321 tgtttgggct cctgagcatt accctggcgg cctccgcgac cggcctgggg gtgctcgcgt
     1381 acggcccctg gatcgcgctt gggggcgcgc tctttccgac ctggccagcg ttcgcggggc
     1441 tggccgcggt ggacgtgctg agcgtggggt tcgcgctcac cgaagggcgt tccccgtccc
     1501 cgctgaccct gctggcgccg gccgcagcgg tgatctgcct gcatctcggg gccgtcctgg
     1561 tctcccgcga ggtccgcgct cgaagccatc tcgcgctcac cgacccgttg accgggctcg
     1621 ccaatcgccg gctgctgggg tggcggctcg gcgaggagct gtcgcatgcc cggcggacgg
     1681 gcgggacgtt tgcgctcatg tacctcgacc tgcaggggtt ccgcgaggtg aacacgaggc
     1741 acggccaccg cgcgggcgat cgggcgctcg tgcaggtcgc ccagatcctc cgggatacga
     1801 tgcgggccca cgacgtcatc gcgcggactt ccggcgacga gttcgccatc ctggcgccgg
     1861 gcctggggga gcccgaggcc gtggtggtcg tggagcggat ccgggccgcc atatcgcgcg
     1921 caacgaccct ccccctcccg ctccggctcg cggccgggtg ggcgatcgcc ccccgcgacg
     1981 gcatggaggc cggggacctc ctcgaaaccg ccgcgtcgag cgtcttcgaa caaaaactgg
     2041 cgtcccgatc ggcgggaccc tcgctgccga cggagctgat gacggcgctg tggagccttc
     2101 ccgagggagc ccagcaactg gtgcggctgc tccacaccga agggatcgag ctcgaagagc
     2161 acctgagcca cgtgggccag tggagcatgg acctcggccg gatggtcggg ctcgacccgg
     2221 cgcggcagac cgcgctggcc caggccgtgc tcgtccacga cgtcggcaag ctcgtggtgc
     2281 ccgtcgccct gctgcgcaag ccgggccccc tcacccgcga cgaggaggcg atcgttgccg
     2341 ggcacgtcac cagtggcgcg gcgctcctgc gggcgacggg ggtcgacgag gccgtggtct
     2401 ccattgtggc cgcccaccac gagcgctggg atggaggcgg ctatcccgcc ggcctggccg
     2461 ccgaccagat tccgcttgaa gcgcgaatcc tcgcgatcgc cgacggctac gacacgatgg
     2521 tggcgcgccg cccgtaccgc aaggcgtgga ccaccaccgc ggcgatcgcg gatgtgcagc
     2581 tcgagggcgg gcaccagttc gaccccgagc ttgtgactct gatcattccc gtcatctcgg
     2641 catcggggta ggtggcgtcc ggcctcaggg gacgaggatc agttttccaa agtgcgcgct
     2701 ctgctccatg agttcgtggg ctcttcggac atcgcggagc gggaagacct cgtgaaccac
     2761 cggacggagc cgtccggcgg agatcagccg aaagatgtcg tggagggtgg cccggctccc
     2821 catgaacgtg ccgcggatca gaagcttccg gctgaacacg tagcggagat cggtctcggc
     2881 cgccggtccg gaggtggcgc cgcaggtcac caaccgccct ccccgcgcca acgccttgag
     2941 gctcgtgtcc catgtggcct ccccgacgtg ctcgaagacg acatccacgc cgcggcggtt
     3001 ggtgaggcgg cggacctcgt ccccgacccg ctgcgtcgcg tggttgatgg tctcatcggc
     3061 gccgagccgg cgcgcccgct cgagcttcca gtcggcgccg gccacggcga gcacccgggc
     3121 gccaaacacc ttggcgatct ggatcgcggc actcccgacc ccgctcccgg ccccccacac
     3181 gaggacggtc tcctccggtt gcacccgcgc gccgacgacc agcatgtccc aggcggtgag
     3241 gaagacgagc aggaccgcgg cggcctcggg gaacgagagg cccgcgggct tggccagcac
     3301 attcgcctcc ggcacccgta cgaactccgc gtagccgccg gcggtgtgct cgcccagaat
     3361 cgcgtactcc cggcacaggt tgtcgttccc cgcaaggcag gcctggcaac gaccgcagct
     3421 caacccgggg gcgatcgcgg cctcgtcgcc cggccgccac gcggtcacct ccgcccccac
     3481 ccgctccacc acccccgcca catcgcagcc gaggatgtgg ggaaaccgca cggtcatccc
     3541 ggggatcccc ctccggacgt ggatgtccag gtggttcatc gcgcacgcgc ggacccggac
     3601 gaggacgtcg cgcgcaccga gcccgggggt gggcgcctcc tcgtagatca gctcttccgg
     3661 cccgccgtgt cggtg
//
LOCUS       DASQGH010000199         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6589, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000199 DASQGH010000000
VERSION     DASQGH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6589"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VEW91_03790"
     CDS             <1..758
                     /locus_tag="VEW91_03790"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYM90744.1"
                     /translation="TADPREIGQVDLVLFCVKTYDTEAAAALIRPIVGPDTVILSVQN
                     GIDNDERIARVVTQGTAISAAAQVNAHVEGPGVIGQTAGPGRLIFGANAGNLGQRNDQ
                     LCRHCKDAGIAAELQPDIKTVLWSKFMFICGFSGVTALTRLTIGPILACAETTALFRG
                     TMAEVDLVAKSQGVALAAGVPDQGLKNSSALEPWAKGSMAHDLALGRRLELESLNGTV
                     VRLGQAKGVPTPLNFAIYAALKPFVNGPPPPSR"
     gene            complement(791..1267)
                     /locus_tag="VEW91_03795"
     CDS             complement(791..1267)
                     /locus_tag="VEW91_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM90745.1"
                     /translation="MATEDALREDVLWLVSGGRAHVGFQRAVADLPEPMRGKRPRGAP
                     YTPWQQLEHMRICQWDILEYIRTPGHKSPDWPEGYWPGPAPAKGAWDKSVRAFQADLR
                     ALKALVSDPKTDLLAKVPHDPKGPTILHEVLLVADHNAYHLGQLIVLRRMLGAWKD"
     gene            1399..1671
                     /locus_tag="VEW91_03800"
     CDS             1399..1671
                     /locus_tag="VEW91_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HYM90746.1"
                     /translation="MLFMVIENFRNQNAKAIYRRFREKGRLTPDGLKFVNSWVEADLG
                     RCFQLMECDDVTLLQRWVAEWSDLVDFEIVPVVSGRETAEGLSGQL"
     gene            complement(1719..1810)
                     /locus_tag="VEW91_03805"
     tRNA            complement(1719..1810)
                     /locus_tag="VEW91_03805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1772..1774),aa:Ser,seq:cga)
BASE COUNT          358 a          697 c          690 g          369 t
ORIGIN      
        1 ccaccgccga tccccgagag atcggccagg tcgacctggt cctcttctgc gtgaagacgt
       61 acgataccga agccgcggcc gccctgatca ggccgatcgt agggcccgac acggtgatcc
      121 tctccgtgca gaacggcatc gacaacgacg agcggatcgc ccgggtggtc acccagggca
      181 cggcgatctc cgccgcggcg caggtgaacg cgcacgtgga ggggccgggg gtcatcgggc
      241 agaccgccgg ccccggccgc ctgatcttcg gggccaacgc gggcaacctc ggccagcgca
      301 acgaccagct ctgccggcac tgcaaggatg ccggcatcgc ggccgagctc cagccggaca
      361 tcaagacggt gctgtggagc aagttcatgt tcatctgcgg gtttagcggc gtgaccgcgc
      421 tcacccggct gacgatcggc ccgatcttgg cctgcgccga gaccacggcg ctgtttcggg
      481 gaacgatggc cgaggtagac ctggtcgcca aatcccaggg cgtcgcgctc gccgcaggag
      541 ttcccgatca aggcctcaag aattcgtcgg cgctggagcc gtgggcgaag gggtcgatgg
      601 cgcacgacct ggccttggga aggcgcctgg agctggagag cctcaacggg accgttgtcc
      661 ggttggggca ggcgaagggc gttccgacgc cgctcaactt cgcgatctac gcggcgctca
      721 agccgtttgt gaacggtccc ccgcccccat ctcgatagcg gcgaaccctg aacgcaccgg
      781 cgcggagtcg ctagtccttc caggccccga gcatccgcct caggacgatc aactgcccca
      841 ggtggtacgc gttgtggtcg gccacgagca gcacctcgtg gaggatcgtg gggcccttgg
      901 ggtcatgggg gaccttcgcg aggaggtccg tcttgggatc ggacacaaga gccttgaggg
      961 ccctgaggtc tgcctgaaac gcccgcacgc tcttgtccca cgctcccttc gcaggcgcag
     1021 gacccggcca gtagccctcc ggccagtcgg gggacttgtg cccgggggtt cggatgtact
     1081 cgaggatatc ccactgacag atgcgcatgt gctcgagctg ctgccagggg gtgtacggag
     1141 ccccccgggg ccgcttcccg cgcattggct cgggaagatc cgccaccgct cgctggaacc
     1201 ccacgtgcgc ccgaccgccg ctcaccagcc acagtacgtc ctcgcgcagc gcgtcctcgg
     1261 tcgccatcca cccacccccg ggcgtgaaat gtgaaccgct tcgtgaaagt gaataacgca
     1321 tgggtagaca cacgtcttcc ccgtgtgggg ggcaggacgt tgccaagagc cccggcggcg
     1381 cggtatcgtg gtggtgccgt gctgttcatg gtgatcgaaa acttccgcaa ccagaacgcc
     1441 aaagccatct accggcggtt ccgggaaaag gggcggctta ccccagacgg cctcaagttc
     1501 gtgaatagct gggtcgaggc cgacctgggc cgctgcttcc aattaatgga gtgcgatgac
     1561 gtcacgcttc ttcagcgctg ggtcgccgag tggtctgacc tcgtcgattt cgagatcgtt
     1621 ccggtcgtat ccggcaggga aaccgccgaa ggcctgtccg gtcagctcta ggctcggagc
     1681 gggggttggc ccgtcggtct cgcccccctc ccttttgctg gcggaggggg ggagatttga
     1741 actcccgata cccttgcggg tatagcggtt ttcgagaccg cccgattcag ccaggctctc
     1801 gcacccctcc gcgtgagcca gtccctattc tacttcgacg ggcctctgct gcctaggctc
     1861 cgttccctga cagcaaacta tgtagcagca accgtgcgac ggggcttctc ttaagccgca
     1921 gcgtggtcct gcctttccac gaccatgaac ccactttgta aagataaggg tgccagtgat
     1981 catacctcgt gtggtgaaga tggaactgcc gctcgtcgag cccgcagggc ggcactgcgc
     2041 tagcggatgc acgtctggaa aggggaatca aatggcggct gtccgtcacg cgcccactct
     2101 cacgcctggt ctca
//
LOCUS       DASQGH010000200         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46231, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000200 DASQGH010000000
VERSION     DASQGH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46231"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1478
                     /locus_tag="VEW91_03810"
     CDS             225..1478
                     /locus_tag="VEW91_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM90747.1"
                     /translation="MNVGRPKSPPLPSPELHAERPAPSAAPARARRPIPRSFLLGAGG
                     FLAAISWQVVLPVLPLHLTKIGYTPTQVGTLVSLLSLAMGLVEMHVGRIVGVFGRRGT
                     LLGGLAANAVCMVFVAHARIASLVGSALATVGVTRGTFWPPLHATVADTASEEARGQA
                     FGMFWFWTSVAYLTGPIIGGVIAAHYGDRAAFYLGAAFSLLAIPVVITVTRPGRPPVE
                     VTAAGAADVLGDPIVFRLCLVNHLYYGMTGIWMTFLPLYIAQQGLSVVIVGWVLTVQG
                     FAYALVQIPTGRLADRYGAHRLVVPGVIGRTIIAPLVPLFHLHTPAAFVVMGALYGFA
                     GGLIPVTFTTLLARMVPRDKYTTAMGVYNSSGDLAFFIGPLIGGAAALLGIGAPFYLC
                     LPIGVAAVLVGASGMALRKRTGEPA"
BASE COUNT          212 a          528 c          526 g          306 t
ORIGIN      
        1 cgatgttacg gtagttcaag actctgaact gcgtaacctt gagactgggc acggtccctc
       61 tcctcggcgt tttggcccat tccaccaccg gtgcgcgtcg cgagcagggc tgacgcgata
      121 tcttatgccc gcccgcgcga ccttctagac ggtcatccat ggccggcacc cttggtggtg
      181 agccctccag gactcctggc ccggtcctcg aacctgtctt cagcatgaac gtgggtcggc
      241 cgaaatcgcc gccgctaccg tcgccggaac tgcacgcaga gaggcctgcg ccatccgccg
      301 cacccgcgcg tgcgaggcgt ccgattcccc gcagcttcct gctcggcgct ggtgggttcc
      361 tcgcagcgat ctcctggcag gtggtgctcc cggtcctgcc cctgcacctg acaaagattg
      421 gctacacccc tacgcaggtc ggcaccctcg tcagcctcct cagcctggcg atggggctgg
      481 tggagatgca cgtgggtcgc atcgtcgggg tgttcggaag gcgggggacg ctgctgggag
      541 ggctcgccgc gaacgccgtg tgcatggtct tcgtggcgca cgcccgcatc gcgtccctgg
      601 tcggatcggc gcttgccacg gtcggcgtga cgcggggcac gttttggccg cccctgcacg
      661 ccaccgtggc ggacaccgcg tcggaagaag cgcgcggcca ggcgttcggc atgttctggt
      721 tctggacgtc cgtggcctac ctcaccggtc ccatcatcgg cggggtgatc gccgcccact
      781 acggcgatcg agccgcgttc tacctgggcg ccgcgttcag cctgctcgct atccccgtcg
      841 tcatcaccgt caccaggccg ggacggcccc ccgtcgaggt caccgcagcg ggcgcggccg
      901 acgtgctcgg agacccgatc gtcttcaggc tctgcctggt caaccatctc tactacggca
      961 tgacggggat ctggatgacg ttcctgccac tgtacatcgc ccagcagggg ctttcggtcg
     1021 tgatcgtcgg atgggtgctc accgtccagg gcttcgccta cgcgttggtc cagattccca
     1081 cagggcggct ggcggaccgg tacggagcgc accggctcgt ggttcccggc gtgatcggcc
     1141 ggacgatcat tgcgccgctc gtgcctctct ttcacctcca tactccggcc gcattcgtgg
     1201 tgatgggggc gctgtatggg tttgcgggcg gcttgatccc ggtgaccttt acaaccctcc
     1261 tggcccggat ggttcctcgg gacaagtaca cgaccgcgat gggcgtgtac aacagttccg
     1321 gcgatctcgc gttctttatc ggcccgctga tcggcggggc ggccgccctc ctggggatcg
     1381 gggcgccatt ctacctgtgc ctgccgatcg gcgtcgccgc ggtcctggtc ggcgcgagcg
     1441 ggatggcttt gcggaaacgg actggggaac cggcctgaac gggtatgcta tactggccgc
     1501 ggagggatgg cagagcgggc gattgcggcg gtcttgaaaa ccgcttgggg gaaacccctc
     1561 gggagttcga at
//
LOCUS       DASQGH010000201         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12235, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000201 DASQGH010000000
VERSION     DASQGH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12235"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            631..1668
                     /locus_tag="VEW91_03815"
     CDS             631..1668
                     /locus_tag="VEW91_03815"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HYM90748.1"
                     /translation="MALTAPQRSIGEGIDRLVSVEITGRGVIGELYAAARRGKETPLC
                     LEAASTLLGRMHKGSVVVLATGLPTYPWFAGEQDGPVGTATLARALVLAVGARPVIVT
                     DPVNVEICAAAVRGAGLYVRSMEDALRLPTTAAVLPFPLAWGEAGTRSEALLDTLRPS
                     ALIAIERPGANEHGQYHSAGGKSLTDHCGKIDALFQTAREREIPTIGIGDGGNELGCA
                     AIREIVLRVVPNGARCTCPCGGTVVPKVSTDLFIAAAISNWGAYGIEAAMAILLERPE
                     VMHTREIDARVHDLCAAAGANNDGPGLLDVGTDAVPAPLHGHIVDLLGQMVRSGIDFG
                     RLYREPRYPWF"
     gene            1678..2724
                     /locus_tag="VEW91_03820"
     CDS             1678..2724
                     /locus_tag="VEW91_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYM90749.1"
                     /translation="MLALPMTREQIRAAIARIPRVRLAHLPTPLQPCPRASRDLGVDL
                     LIKRDDLTGLAFGGNKTRNLEFRIAEARDSGADVLVFGVEVGSNSARQTAAAANLLGL
                     PIVLVLRGRPDTPVDGNLLVDYLLGAEVRLIDLAPDADLDAEVDRVAAALREQGRRPF
                     NLNTARMFARASALAYIEALLEIQEQLGDRRADFLYMCSGSKGLAGLLLGQRLTGEPA
                     RIVAVSATHGHGDLREAASRIATETAELLRVGPVRLTPREVELTTDYVGRAYGIPTAA
                     GNAAILYLARKESILLDPVYTGKAFAGLLDHVRTGRIPQGARVVFVHTGGQPALFSFE
                     RELMAAAQDPQERG"
     gene            2729..4324
                     /locus_tag="VEW91_03825"
     CDS             2729..4324
                     /locus_tag="VEW91_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90750.1"
                     /translation="MTGGRLLAAGVIAAVAAAAIGPRTVGAQAPSTLTVAQSVAVTAL
                     DPAGPSIFLVYPAGYEAAFLVYDGLVRFSEGMIVAPALATSWSVAPDGRTWTFKLRGE
                     VSFQDGTPLTAQAVAEDFQREVNPQTNQSNRPLWDPIGSVSAPDAATVQIVTREPYGA
                     LLNTLAHGSALIASPRAVEKWGGQYKLHPVGTGPYEVNRWDVGTQLEMVRTSQYWGGR
                     PGFDRIVLRNVPDPTTRVAMIQSGQAQVAEGIPPENVDELRRVPGTTVIVRPALRTFG
                     MGINLTRPALQDIRVRQALNYAVNKDLIVKALFRGNATVLHSPLAPQASGYADLGPWP
                     YDPPKARALLEEAGWKPAPPIGVRVRDGKSLQLTLLTPQGAFPHDVEVIETLADYLRN
                     VGFEPHFLYVEPAVFWDHLLIPPDQYTWDLVLFGFNPSNGDGGYHLDSLYRSNPDRQH
                     RPRVWNFTWYSNPQVDAWLTEGSRAIDPKARAATYARVERQVWNDAPYLWLYAENVIV
                     ATRDVSGVEVLPIVFTILRSAYR"
     gene            4344..>5012
                     /locus_tag="VEW91_03830"
     CDS             4344..>5012
                     /locus_tag="VEW91_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90751.1"
                     /translation="MPAYLTGRLLQLAPHGLLVLTLIFVLFRLVPGDPAVLASGLTAT
                     PEQIAAMRHLLGLDRPLAVQYGAFLWQLLHGDLGTSVTFGLPVLGVVSHRVPATVALG
                     GSSLLVAVGVGIPFGVLAAVRPRGIGGQAATATAVALLAIPNFWLGLLLIHLFAVRLR
                     WLPVAGTGGIAFILLPTFAIAARLIAVVSRTTRASLVEVLSEDYIRTARAKGLARGPL
                     LLRHV"
BASE COUNT          757 a         1744 c         1714 g          797 t
ORIGIN      
        1 tataagccat gagattaggg cacgggggtc ttgccatgag aactgtcaat gtagcggacc
       61 tgaaggacag gctgagcgcc tacctcagac aagtccgcgg gggcgaagaa attctggtca
      121 gggatcggac ccgtcccata gccttgatcg ttccgttgtc agcccgagcg agcggcgatg
      181 ccgatgtcga agctctagtc gcgtcaggtc gggcgcgccc agcgcaggca tcactctcgt
      241 cggccttctg ggcgctccca gcacctcgca tcccagtcgc gcgggcatcg agggctgtta
      301 cggccgatcg tgatgagccc gagtagcgcg ttctgggaca cgagcgctat cgttcttctc
      361 tgcttctatc aagacgccag cagcgcctta cggcggatga gccgtgcata ttcacccatg
      421 gttgtctggt ggggtgcccc agtagaagcg tcagcgcgtt cgcgcgcttg gtacgcgagg
      481 gtacaatgag cgaaataggc ctgcaccaag agcacgaact cgcgcagaaa gtttccgggg
      541 tggaactcac aggacagaag gaacgcagcg cccccccggc atagagtaga gcggttgcgg
      601 cactagaggg cacacaggag gcacgcccgc atggcgctca ccgcccccca gcgcagcatc
      661 ggcgagggga tcgaccggct ggtcagcgtt gagatcaccg ggcgcggcgt gatcggagag
      721 ctctacgccg cggcgcggcg ggggaaggag accccgctgt gcctcgaggc cgcgtccacc
      781 ctcctgggac ggatgcacaa gggcagcgtg gtggtcctgg ccacaggcct accgacctat
      841 ccgtggttcg cgggcgagca ggatgggccc gtcggtaccg cgaccctggc gcgcgccctc
      901 gttctggcgg taggcgcgcg gcccgtgatt gtcaccgacc cggtcaacgt ggagatctgc
      961 gcggccgccg tgcggggcgc gggtctctac gtccgatcca tggaggacgc gctgcggctt
     1021 cccacaacgg cggccgtcct tccattcccc ctcgcgtggg gcgaggcggg gacgcgctcg
     1081 gaggcgctcc tcgacacgct gcgcccctcg gccctcatcg cgatcgagcg gccgggcgcc
     1141 aacgagcacg gacagtatca ctcggccgga gggaagagcc tgaccgatca ctgcggaaag
     1201 atcgacgcgc tgttccagac ggcgcgggag cgggagatcc ccacgatcgg catcggtgac
     1261 ggcggcaacg agctcggctg cgccgcgatc cgggagatcg tcctgcgcgt cgtccccaac
     1321 ggcgccaggt gcacctgccc gtgcggtggg acggtggtcc cgaaggtctc caccgatctc
     1381 ttcatcgcgg ccgcgatctc caactggggg gcgtacggga tcgaagcggc catggcgatc
     1441 ttgctcgaac gccctgaggt gatgcacacc cgcgagatcg acgcccgcgt gcacgatctc
     1501 tgcgcggccg ccggggccaa caacgatggc ccgggactgc tggacgtcgg gaccgatgcc
     1561 gtcccggctc ccctccacgg ccacatcgtc gacctgctgg gccagatggt gcggagcggg
     1621 atcgactttg gacggctcta ccgcgagccg cgctatccat ggttctgacc gggtgagatg
     1681 ctggcgctgc cgatgacgcg ggaacagatc cgggccgcca tcgcgcggat cccgcgggta
     1741 cggctggccc atctgccgac cccccttcaa ccgtgcccgc gggcgtcgcg cgatctcggc
     1801 gtcgacctcc tcatcaaacg ggacgacctg acggggctgg cgttcggggg caacaagacg
     1861 cgcaacctgg agttccggat cgccgaggcg cgcgacagcg gcgccgatgt gctcgtgttc
     1921 ggggtggagg tcggatcgaa ctcggcccgg caaacggccg cggcggccaa cctgctcggg
     1981 ctacccatcg ttctcgtgct ccgggggcgc ccggataccc cggtggacgg gaacctgctc
     2041 gtggattacc tcttgggcgc ggaggtccgc ctgatcgatc ttgcgcccga cgcggatctg
     2101 gacgctgagg tcgatcgggt ggccgcagcg ctccgcgagc aggggcggcg gccgttcaac
     2161 ctcaacaccg cccggatgtt cgcccgggcc tccgccctcg cctacatcga agcgctcctg
     2221 gagatccagg agcaactggg ggaccggcgc gcggacttcc tttacatgtg ctccggctcc
     2281 aaggggctgg ccggcctgct cttgggccag cggctcacgg gggagcccgc ccggatcgtc
     2341 gccgtctcgg ccacccacgg gcacggtgat ctgcgggaag cggcatcgcg gatcgccacc
     2401 gagaccgccg agttgctccg ggtgggaccc gtgcgactca ccccccgcga ggtggaactt
     2461 accaccgact acgtgggccg agcctacggg atcccgacag cagcggggaa tgccgccatc
     2521 ctgtacctcg cgcgcaagga aagcatcctc ctggatcccg tctacacggg gaaagccttc
     2581 gccggcctgc tcgaccatgt ccggaccgga cggatcccgc agggcgcccg ggtcgtcttc
     2641 gtccacacgg gcggccagcc ggccctgttt tccttcgagc gggagttgat ggcagccgcc
     2701 caggatccgc aggagcgggg gtgacgcgat gaccggaggt cgcctcttgg ccgccggcgt
     2761 gatcgccgcc gtggcggcgg cggcaatcgg gccgcggacg gtcggggccc aggccccttc
     2821 gaccctcacg gtggcccaga gcgtcgccgt gacggctctc gatccggcag ggccgtcgat
     2881 cttccttgtg taccctgccg gatacgaggc agcctttctg gtgtacgatg gcctggtccg
     2941 gttcagcgag gggatgatcg ttgcccccgc gctggccaca tcgtggagcg tggccccaga
     3001 cgggcgtacc tggacgttca aactccgggg cgaagtgtcg ttccaggatg ggacaccgct
     3061 cactgcccag gcggtggcgg aggatttcca gcgggaggtc aatccccaga ccaaccagtc
     3121 caaccgcccc ctgtgggacc cgatcgggtc ggtctccgcc ccggacgcgg ccacggtcca
     3181 gatcgtgacc cgggaaccct atggagccct cctcaacacc ctcgcccacg gctccgccct
     3241 gatcgcgagc ccccgggcgg tggagaaatg gggggggcag tacaagttgc atccggtcgg
     3301 cacgggaccc tacgaagtga accgctggga tgtcggaacg caactcgaga tggtccggac
     3361 ctcccagtat tggggcggac ggccggggtt cgaccggatc gtcttgcgca acgtgccgga
     3421 ccccaccacc cgcgtcgcga tgatccagag cggccaggcc caggttgcgg aggggatccc
     3481 gcccgagaac gtggacgaac tccggcgagt gcccggcacc acggtgatcg tcagacccgc
     3541 gttgcgcacc ttcggcatgg ggatcaactt gacccgcccg gccctgcagg atatccgggt
     3601 gcgccaggcg ctcaactacg cggtcaacaa ggacctcatc gtgaaggcgc tgttccgggg
     3661 aaacgcgacg gtgctccact ccccgctcgc gccgcaggcc tcgggctacg ccgacctggg
     3721 cccctggccg tacgatccgc cgaaggcccg cgccctcctg gaggaggccg gatggaagcc
     3781 ggcgcccccg atcggggtcc gcgtcaggga cggaaagtcc ctgcagctca ccctcctcac
     3841 cccccagggc gcgttccccc acgatgtgga ggtgatcgag acgctggccg attacctccg
     3901 caacgtgggg ttcgagcccc acttcctgta cgtcgagccc gccgtgttct gggaccacct
     3961 gctgatcccc cccgatcagt acacgtggga cctcgtcctc ttcgggttca accccagcaa
     4021 cggcgatggg gggtatcatc tcgactcgct gtaccggtcg aaccccgacc ggcagcaccg
     4081 tccccgcgtc tggaacttca cctggtactc caacccccag gtcgatgcct ggctcaccga
     4141 aggcagccgc gccatcgatc ccaaggcgcg ggcggccacc tacgcccggg tggagcgcca
     4201 ggtgtggaac gatgcgccat acttgtggct gtacgccgag aatgtgatcg tcgcgacgcg
     4261 ggacgtgagc ggggtcgagg tcctccccat cgtcttcacg atcctccggt cggcgtatcg
     4321 gtagcccgcg ccgacccgcg ggcatgccgg cgtacctcac gggccgcctc ctccagctcg
     4381 ctccgcacgg tcttctggtg ctcacgctga tctttgtgct cttccggctg gtgccgggag
     4441 acccggccgt cttggcgtcg gggctgacgg cgacccccga gcagatcgcc gcgatgcgcc
     4501 acctcctcgg gctggaccgg ccgctcgcgg tccagtacgg tgcattcctc tggcagctgc
     4561 tccacggcga tctcggcacc tctgtgacct tcggcctgcc cgtcctcggc gtggtcagcc
     4621 accgggtgcc ggcgaccgtg gccctggggg gcagcagcct cctggtggcc gtcggtgttg
     4681 gcatcccatt cggggtcctc gccgccgtgc gccctcgggg gatcggcggg caggcggcga
     4741 ccgccaccgc cgtcgcgctg ctggcgattc ccaacttctg gctaggactg ctgttgatcc
     4801 acctgttcgc ggtgcggttg cgatggctgc cggtcgctgg cacgggcggg atcgcgttca
     4861 tcctgctgcc gaccttcgcg atcgccgcgc gactcatcgc ggtcgtgagc cggaccacgc
     4921 gggccagcct cgtggaagtc ctgagcgaag attacatccg gacggcgcgg gccaagggcc
     4981 tggcccgagg tccgttgctc ctccggcatg tc
//
LOCUS       DASQGH010000202         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46241, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000202 DASQGH010000000
VERSION     DASQGH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46241"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(731..822)
                     /locus_tag="VEW91_03835"
     tRNA            complement(731..822)
                     /locus_tag="VEW91_03835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(786..788),aa:Ser,seq:gct)
     gene            892..981
                     /locus_tag="VEW91_03840"
     tRNA            892..981
                     /locus_tag="VEW91_03840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:926..928,aa:Ser,seq:tga)
     gene            complement(1068..1235)
                     /locus_tag="VEW91_03845"
     CDS             complement(1068..1235)
                     /locus_tag="VEW91_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYM90752.1"
                     /translation="MASNPRRLGRSLRFELEGCWSARRGRYRIIYRIDKATRSIKVLA
                     VAHRADVHRPR"
     gene            complement(1263..>1828)
                     /locus_tag="VEW91_03850"
     CDS             complement(1263..>1828)
                     /locus_tag="VEW91_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HYM90753.1"
                     /translation="SLFDNAASPPVRSESRGIVVRLDPQQGTATLVSQLTHSPPLLAY
                     SQGNLQALGNGDWFLGWGQVPYFSELGPQGQLLFDAHFPAHTQSYRSFRFPWTGTPAH
                     PPSFVLQPAAGRRATVYASWNGATLVSAWRVLAGPTAAGLRPVAQAPRGGFETAIPLP
                     AGTAGPYVAVQALGAGGQVLGVSAAHP"
BASE COUNT          316 a          644 c          615 g          253 t
ORIGIN      
        1 caagcgcatc ctccgcatgc ttcgcatccg cacccaagcc gcagctcgaa ggcgcagcgg
       61 gcaaatgggg cttctcgccg cgcagctgct ccccgttccc gggcacgcgc agccggcagc
      121 cgctctccgc tcgcgaggaa gctagaacca acacgatcgc cgcgcagacg cccagcgcgg
      181 cagccgcgat ggctatccac acgggcctcc gcgcaagcac aacacgcatc ggcggccgac
      241 cctgctcgcc ctccgacata cccccagtca ttccgccggg cagcctccca ggcgcaccag
      301 accgaggcaa taggttcaca ccgagtcaac aagccaattc ccgcaaacag cggtctttgc
      361 cgcctcaccg gcgctggtgc gcccatccgt acggcttccc gcacgccaga agatcgctcc
      421 cgcagatcgg ccacgcccca ttgctcagcc caccccgagg tcagagagta cgagcaagca
      481 ctcgacgacg ccctcctgct catcgacaag ggtcagatcc cctacctcac cggatcaccc
      541 gccgagaaac ggctcatcaa cctgaccatc tacgacatgc tcctcgtctc cgccaccggc
      601 gacgtcgagg ccaagcccac ggccctctac gccgacgtca tcccgctcgc cccgcaagct
      661 ggcccgggaa gccggcccca agacggccgc ggccccgaca gtcggggccg cggtttgaaa
      721 gcaaagcaaa tggcggagag ggcgggattc gaacccgcga tggagtttga cccccatact
      781 cgcttagcag gcgagtgcct tcagcctctc ggccacctct cctgagatcg ggacgccagt
      841 gtagagcctg ccgggcatgc cgcgcgagcg cgtagcatct gggaatcaca cggaagggtg
      901 gcagagcggt tgaatgcacc ggtcttgaaa accggcaggc gggcaacccc cgtctcggga
      961 gttcgaatct ccccccttcc gttttcgccg ggtgtgaggc tgttattcaa actcgccccc
     1021 ggcctcctgg ctgggcctcg tcggatcaaa gcgtattggt agatcactca gcgtgggcgg
     1081 tggacatcgg cgcggtgcgc gacggcaagg accttgatcg agcgagtcgc cttatcgatg
     1141 cgatagatga tccggtagcg cccgcggcgc gctgaccagc acccctccag ctcgaagcgc
     1201 agagacctac cgaggcgtcg ggggttggat gcgatcgcgt cgagcgtgcg gccggcttgc
     1261 cgttacgggt gagccgcgga cacccccagc acctgcccgc cggcgcctag cgcctggacg
     1321 gccacgtagg ggccggccgt cccggccggc agggggatgg cggtctcaaa gcctccgcgg
     1381 ggcgcctggg cgaccggcct cagccccgcc gcggtggggc ccgcgaggac cctccaggcg
     1441 gacacgagcg tggcgccgtt ccagctggca tagacggttg ccctccggcc ggcggccggc
     1501 tggaggacga aggacggggg gtgggcgggc gtacccgtcc aggggaagcg gaagctccgg
     1561 taggactggg tgtgggccgg gaagtgggcg tcgaacagga gctggccctg ggggccgagc
     1621 tcggagaagt aggggacctg gccccagccg aggaaccagt cgccgttgcc gagcgcctgg
     1681 aggtttccct ggctgtaggc gagcagcggc ggggagtggg tgagctggct gacgagggtg
     1741 gccgtgccct gctgaggatc gagcctcacg acgatgcccc gcgattcgct gcgcaccgga
     1801 ggggaggcgg cgttgtcgaa caggctga
//
LOCUS       DASQGH010000203         6285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54640, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000203 DASQGH010000000
VERSION     DASQGH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54640"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VEW91_03855"
     CDS             complement(<1..1207)
                     /locus_tag="VEW91_03855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HYM90754.1"
                     /translation="MRLTDLLAGRLASRALVGYPDCEISGIAYHSGAVRPGDLFAAIR
                     GFVHDGHQYAAEAVRRGARAVLVDHALPDVAATQVVVPDTREALAALASAFYGDPSHD
                     LWVCGVTGTNGKTTTTYLIDAVLRAAGHRAAVIGTTGILAEGAPVPFSMTTSTTPEAP
                     DLQRLLRDLSGEGIDHIVMEVTSHALELSRVASCHFRAAVFTNLTQDHLDLHGDLARY
                     RDAKSRLFAMVDPAGASIVNADDPASAAMGQRSRARVVTYGIDRPAEIRATAIALAPD
                     GTQCTVTWPEGTRRLSLRLPGLFNVSNALAAFAAGLACGADPVVIAQALGSVAGVPGR
                     CELVDEGQPFTVLVDYAHSPDSLEKILRLAKQISSGRRIVVFGCGGERDRTKRPIMGR
                     IGTQLADHAI"
     gene            complement(1328..3058)
                     /locus_tag="VEW91_03860"
     CDS             complement(1328..3058)
                     /locus_tag="VEW91_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYM90755.1"
                     /translation="MIKRPETSPPGAPPGLSRWRLTAVFAVWITTFALLAVRLVELQV
                     VHASALQRLAIRQHLESLRLPGRRGLILDRRGSRLAVNVEAASVYAVPRMIPDRAAFA
                     REVAPRLQLSAGEVEARLQRGGPFFAWLARRQPPETADAIRALHLGDALGIMPESLRT
                     YPAGALAAGAIGFTGLDGVGLAGLELQYDGTLHGVEGIGTAHRDAIGRELMQTQRILA
                     PPRDGDTLVLTLDEVVQHIAQRELGRTVTATGAQGGVAIVMDPQSGAILALAALPTFD
                     PNQYQAAPPSHWKSRAVSDLYEPGSTFKLFLAAAALGTQAVDPDETFLDPGKIRINGA
                     TIHDAEPPEHSILTLAEIVKYSSNVGAAQVATRVGKDRFDAYIRRFGFGRPTGIDLPG
                     EAAGIIRPTSEWYGPSLQNIGFGQGISVTPIQLLRAASALATHGLAVHPHLVEAIRDP
                     AGHTVATPGERPPDQVIEPGVADRVLSMMRSVVDGGTGIQARLEGYPVAGKTGTAQRP
                     GAFGYEPGAYVASFVGVVPVPGPRLAILVIVDRPRGVYLGGEVAAPVFREIARQVLWY
                     LRIPPAGGPE"
     gene            complement(3055..3354)
                     /locus_tag="VEW91_03865"
     CDS             complement(3055..3354)
                     /locus_tag="VEW91_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90756.1"
                     /translation="MSNAAHIGYQILQETQDIAALESEHERLQAIASSLRAPDRIERL
                     ATARLGLSAPRAGQIAVLSLPVVASREESPKPPGLWERLGDYFHRSEAAAAEPPQ"
     gene            complement(3522..4496)
                     /gene="rsmH"
                     /locus_tag="VEW91_03870"
     CDS             complement(3522..4496)
                     /gene="rsmH"
                     /locus_tag="VEW91_03870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYM90757.1"
                     /translation="MSPTAKRETTAHVPVLVEEVLTYLAPRPGAVIVDATVGEGGHAE
                     AILQRIAPAGRLIGLDRDADAVLRAEERLRRYGQNVTLRQANFAALRSVLSEVGVGLV
                     DGVLFDLGVSTRQLLEPERGFSFEREGPLDMRMDPQQAVTAAELVNTLSERELAALIY
                     RYGEERASRRIARQIVARRPLRTTRDLARAVEAAIGSGRGRLHPATRTFQALRIATNA
                     ETDALASGLPQAIECLRPGGRLCVISFHSLEDRIVKQALTAGARGCTCPPDVPVCTCG
                     RQRTLRVITKKPVTPTLEEVRRNPRARSAKLRAAERLRDARPEPHDLP"
     gene            complement(4530..4949)
                     /gene="mraZ"
                     /locus_tag="VEW91_03875"
     CDS             complement(4530..4949)
                     /gene="mraZ"
                     /locus_tag="VEW91_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYM90758.1"
                     /translation="MLRGEAEYALDEKGRVVIPPKFRAALGERLIVTRWIDPCLSVFP
                     PAAWQSLEEKIRDRPVKNMEFVRMVWAAEDCELDRQGRIFIPPHLREYAGITRAVTII
                     GMGTRLELWSTPVWRSRMKKIAKAPEALVEHLQDFIL"
     gene            complement(5102..5608)
                     /locus_tag="VEW91_03880"
     CDS             complement(5102..5608)
                     /locus_tag="VEW91_03880"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HYM90759.1"
                     /translation="MVIGWARGGRTGALERTSLRALPLLGAAVLLVLLARLPVLAPDA
                     ARGVGAAAYLAALGFLWQNRAQPWMLVVFLGLALNSLAMFLNGGRMPVSGEALARAGH
                     LGNPGGHGGLGWRYVLAGPGTRMAPLGDTLPVRVGSAGVVVSPGDLLMALGIAGFVQG
                     RMRAAPPA"
     gene            complement(5635..5901)
                     /locus_tag="VEW91_03885"
     CDS             complement(5635..5901)
                     /locus_tag="VEW91_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90760.1"
                     /translation="MRCPNCGKRSVISHVTVRWSQVRGALLPARGHYCTACKTAFEDA
                     RPRTGEFAFRSIRVDAMTDAIRRLQESADLIVVRHKPGAREVLF"
     gene            6086..>6285
                     /locus_tag="VEW91_03890"
     CDS             6086..>6285
                     /locus_tag="VEW91_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90761.1"
                     /translation="MTEDLRDSQNSTSGTDERPGRLTRREILAAGAAGLAGAALGAVG
                     VDRLAGRAEGAAYGVPGMQMEFP"
BASE COUNT          962 a         2241 c         2198 g          884 t
ORIGIN      
        1 agatcgcgtg gtcggcgagc tgggtgccga tccgtcccat gatcggccgt ttcgtccgat
       61 cccgttcgcc cccgcaccca aacacgacga tgcggcggcc cgaggagatc tgcttcgcca
      121 ggcgcaggat cttctccagg ctgtccgggg agtgcgcgta atcgacgagg acggtaaacg
      181 gctgcccctc gtccaccagc tcgcaccgcc ccggcacgcc cgccacggac ccgagtgcct
      241 gggcgatcac caccgggtcg gcgccgcacg cgaggcccgc cgcaaacgcg gcgagcgcgt
      301 tggacacgtt gaacaggccg gggaggcgca gcgacagccg gcgggtgccc tccggccaag
      361 tcacggtgca ctgcgtcccg tccggcgcca gcgcgatcgc ggtcgcccgg atctccgccg
      421 ggcggtcgat cccgtaggtg accacgcggg cgcggctccg ctgccccatc gccgcgctcg
      481 ccggatcgtc ggcgttgaca atgctcgcgc cggccggatc caccatcgca aacagccggc
      541 tcttcgcgtc gcggtagcgg gcgaggtccc cgtggagatc cagatgatcc tgggtcaggt
      601 tcgtgaagac ggcggcgcga aagtggcacg acgccacccg gctcaactcg agcgcgtgcg
      661 acgtgacctc catgacgata tggtcgatcc cctcccccga caggtcgcgc agcaggcgct
      721 gcagatcggg ggcttcgggc gtcgtggagg tcgtcatgga aaacggcacc ggggcgccct
      781 cggccaagat gccggtcgtg ccgatcacgg ccgcgcgatg ccctgccgcc cgcagcaccg
      841 cgtcgatcag gtaggtcgtg gtcgtcttcc cgttggtccc cgtcaccccg cacacccaaa
      901 ggtcgtgcga cgggtccccg tagaacgcag atgcgagcgc ggcgagggct tcgcgcgtgt
      961 cggggacgac cacctgggtg gcggccacat cgggcagcgc atggtcgacg agcaccgccc
     1021 tcgcaccccg acggacggcc tccgccgcgt attggtgtcc gtcgtgcacg aatccgcgga
     1081 tcgcggcgaa caggtccccg ggccggacgg cgccggagtg gtaggcgatc cccgagatct
     1141 cgcagtcggg gtatcctacg agcgcacggg acgcaagccg ccccgccagc aggtcggtca
     1201 accgcattct ggcctcccgt gcaccctctc ccgccgggtc ccgcccgagc ggatccccgg
     1261 cactcccccg cggctcggcc gtccccttca acctactata ttagcaattt ggccctgcgc
     1321 gtagacatca ttccgggccg ccggccgggg gaatccggag ataccacagc acctgccggg
     1381 cgatctctcg aaacaccggc gcggcgacct ctccccccag atataccccc cggggccggt
     1441 ccacaatcac gaggatggca agccgcggcc cgggcacggg gaccaccccg acaaacgacg
     1501 cgacatacgc ccccggctcg tacccgaagg ccccgggccg ctgcgcggtc ccggtcttgc
     1561 cggccaccgg gtacccctcg agccgcgcct ggatcccggt cccgccgtcg acgaccgatc
     1621 gcatcatcga cagcacccga tcggccaccc cgggctcgat gacctggtcg ggagggcgct
     1681 ctccgggggt ggccaccgta tggcccgccg gatcgcgaat ggcctcgacc aagtgcgggt
     1741 ggacggcgag cccgtgggta gcaagggcgg acgcggcccg gagcagctgg atcggcgtga
     1801 ccgagatccc ctgcccgaac ccgatgttct gcagggacgg cccataccac tcgctggtcg
     1861 gacggatgat ccccgccgct tctcccggca gatcgatccc cgtcggccgc ccgaatccaa
     1921 accgccggat gtacgcgtcg aatcggtctt ttccgacccg ggtcgccacc tgggcggccc
     1981 cgacgttgct cgaatacttg acgatctccg cgagcgtcag gatggagtgc tcgggcggtt
     2041 cggcgtcgtg aatcgtcgcc ccgttgatcc ggatcttgcc cgggtccaag aacgtctcgt
     2101 cggggtcgac cgcctgcgtg ccgagggcgg cggcggcgag gaacaacttg aacgtcgagc
     2161 ccggctcgta cagatcgctg acggcccggc tcttccagtg cgatgggggc gcggcctggt
     2221 actggttggg atcaaaggtg ggaagcgcgg cgagggccag gatcgcgccg ctctgcgggt
     2281 ccatcacgat cgccacccca ccctgggcgc cggtggcggt cacggtccgg ccaagctcgc
     2341 gctgggcgat gtgctgcacg acctcgtcca gcgtgagcac cagggtgtcg ccgtcgcggg
     2401 ggggcgcaag aatccgctgg gtctgcatca gctcccggcc gatcgcgtcg cggtgggccg
     2461 tgccgatccc ctccaccccg tgcagcgtgc cgtcgtactg cagctcgagg ccggccaggc
     2521 cgacgccgtc caggccggtg aacccgatcg cgccggccgc gagcgccccc gcggggtagg
     2581 tgcggaggct ctcgggcatg atgccgaggg cgtcccccag atgcagcgcc cggatcgcgt
     2641 cggccgtctc ggggggctgg cggcgtgcga gccacgcgaa gaacggcccg ccgcgctgga
     2701 ggcgggcctc cacctcccct gcggagagct ggaggcgggg ggccacctcg cgggcgaacg
     2761 ccgcgcggtc ggggatcatc cggggaaccg cgtagaccga ggcggcctcc acgttcaccg
     2821 caagccggga cccgcggcga tccaggatca gtccgcggcg gccgggcagc cggagcgact
     2881 ccaggtgctg gcggatcgcg agccgctgca gcgcgctcgc gtggaccacc tggagctcga
     2941 cgagccggac cgccaggagc gcgaacgtgg tgatccacac cgcgaagacc gcggtgagcc
     3001 gccaccggga gaggccggga ggcgcacccg gaggggaggt ctcgggccgc ttgatcattg
     3061 cggcggctcg gcggccgccg cttcactccg atggaagtag tcgccgagcc gctcccacag
     3121 cccgggtggc ttgggggact cctcgcgcga cgccactacg gggagcgaga gcacggcgat
     3181 ctggcccgcg cgcggagcgg aaagccccag acgggcggtg gcgaggcgct cgatacggtc
     3241 cggggcccgg agggaggagg cgatggcctg gaggcgctca tgttcggatt cgagcgcggc
     3301 gatgtcctgg gtctcttgga ggatctggta gccgatgtga gcggcgttgg acactgctgc
     3361 aacgtatgcg atgaatggga gcacggcgag tgctgcgatg gtgagtcctg agagtacagg
     3421 ggagcgcttc cgccgacgcc gagtcgcctc cttccctcgt gggccccaat gctccgggat
     3481 gagcatcggg gacgagatgt cgtggtgcca agcagcctgc ttcatggtag atcgtggggc
     3541 tccgggcgtg cgtccctcag ccgctccgcc gcgcgcagct ttgcgctgcg cgcgcgcggg
     3601 tttcggcgca cttcctcaag cgtcggggtg accggtttct tggtgatgac gcgcagcgtc
     3661 cgctgcctgc cgcaggtgca gaccgggacg tccggggggc aggtgcaccc ccgcgcgccc
     3721 gcggtcagag cctgcttgac gatacggtcc tcgagcgagt gaaacgagat cacgcagagc
     3781 cggccgccgg gccgcaggca ctcgatggcc tgcgggagcc cggaggcgag cgcatcggtt
     3841 tccgcgttcg tggcgatgcg gagcgcctgg aacgtccggg tggcgggatg caggcgaccc
     3901 cgcccgctgc cgatggcggc ttccaccgcg cgcgcgaggt cgcgggtcgt ccgcaggggt
     3961 ctccgcgcga cgatctggcg ggcgatccgc cgcgaggcgc gctcctcgcc gtaccggtag
     4021 atgagcgcgg ccagctctcg ctcgctcaag gtgttcacca gctccgccgc cgtgaccgcc
     4081 tgctgtgggt ccattcgcat gtcgagcggg ccctcgcgct cgaagctgaa tccgcgctcg
     4141 ggctcgagga gttggcgggt cgacacccca aggtcgaaca gcacgccgtc gacgagcccc
     4201 acccccacct cggagagcac cgatctgagc gcggcgaagt tggcctgccg gagggtgacg
     4261 ttctggccgt agcgacgcag ccgctcctcg gcgcggagaa cagcatccgc gtcccgatcc
     4321 agtccgatca accgtcccgc gggggcgatc cgctggagga tcgcctccgc gtgcccgcct
     4381 tcgccgaccg tcgcgtcgac gatcaccgcc cccggccggg gcgccagata ggtgagcacc
     4441 tcctcgacca ggacggggac atgcgccgtg gtctcgcgtt tggccgtcgg gctcatccac
     4501 aggaccgcca tgagcgtgat gcacccaccc tacagaatga agtcctgaag gtgctcgacc
     4561 aaagcctcgg gcgccttcgc gatcttcttc atccggctcc gccagaccgg cgtactccac
     4621 agttccagcc gcgtgcccat cccgatgatc gtcacggcgc gggtgatccc ggcatactct
     4681 cgaaggtgcg gtgggatgaa gatccggccc tgacggtcca gctcgcagtc ctcggcggcc
     4741 cagaccatcc tgacgaactc catgttcttg accggccgat cacggatctt ctcttcgagc
     4801 gactgccagg cggccggcgg gaacaccgaa aggcacggat cgatccagcg cgtgacgatc
     4861 aggcgctcgc ccaaggccgc gcgaaacttg ggtgggatca ccacccgccc tttctcgtcc
     4921 agagcgtact cggcttcgcc cctgagcatc cgatcccgcg cccatttgta tccccactgg
     4981 tccctacgag cctccacatt tctccacaat tcccccttat cctgcattga gccaaaagtc
     5041 ttccaaaaaa atttctgacc ccggtgaggg gccaggtccg ggcgcacatg tgttcgagtc
     5101 tctacgcggg cggtgcggcg cgcatccggc cctggacgaa tccggcgatc cccagggcca
     5161 tcaggaggtc cccggggctg accaccacgc cggcgctccc gacgcgcacc ggaagcgtgt
     5221 cgcccagcgg cgccatccgg gtgccggggc cggcgagcac gtaccgccag ccgagccccc
     5281 cgtgcccccc gggattgccg aggtgccctg ccctcgcgag cgcctcgccg gagaccggca
     5341 tccgcccccc gttgaggaac atcgccaggc tgttcagggc gaggccgagg aaaacgacga
     5401 gcatccaggg ctgggcacgg ttctgccaga ggaatccgag cgcggccagg taggcggccg
     5461 ccccgacccc gcgcgcggcg tccggcgcga gcacgggaag ccgggccagc aggaccagca
     5521 gtacggcggc tcccaggagc ggcagggccc tgagcgaggt gcgctcgagc gccccggtgc
     5581 gtcctccccg cgcccaccca atgaccaccc cggcgagcgc ggcgaggaag aggatcagaa
     5641 caggacctct ctcgcgcccg gcttgtggcg cacgacgatg aggtccgcgc tttcctgaag
     5701 acggcggatc gcgtcggtca tcgcgtcaac gcggatggat cgaaacgcaa actctcccgt
     5761 tcgaggacgc gcatcctcga acgccgtctt gcacgcggtg cagtagtgcc cccgggcggg
     5821 cagcagggct ccccggacct gactccagcg gacggtgacg tggctgatga cgctccgctt
     5881 cccgcagttg ggacaccgca tggcagcccc attatagcac tgggggggtg gtcaagcctc
     5941 cggcgccccg ggcgccgccg cgattcagga aaccattccg ccaccgcacc cgttccatag
     6001 gggagcaagg aaccggaggc gaatgagagc gggacaggga ttgcgtgcgg acccgtttcc
     6061 agggcccaca aagggggggt gcaagatgac cgaggacctc agggactccc agaactcgac
     6121 ctcaggcacc gatgagagac ccggacgcct gacgcgccgg gagatcctcg cggccggagc
     6181 cgccggcctt gccggagcgg cgcttggcgc ggtaggggtc gaccgcctgg cgggaagggc
     6241 ggaaggcgcg gcctacggcg tcccgggcat gcagatggag tttcc
//
LOCUS       DASQGH010000204         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51740, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000204 DASQGH010000000
VERSION     DASQGH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51740"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1512)
                     /locus_tag="VEW91_03895"
     CDS             complement(79..1512)
                     /locus_tag="VEW91_03895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM90762.1"
                     /translation="MRRYTGLTLIIVIAAALRFALLGHNSLWDDEAFVAHAIQKGWHD
                     LIVFLAAHDNHPPLYFALIKAWTGIAGTGEAALRAPSAMFGVVSVPLTYAIMRRLSGD
                     RAARIAALFVAVSPFEVMAGQNARMYTLLEMLTLASTLLLILPTRRLWHSTAYVGTAA
                     AMMYTHYLGFVVLAAHGIWTAWRDRRRLMEWAIGAGVAGILFIPWMPYFFDQVVKRHV
                     WGWIHPLLDVGGLFAFGGSLFGLGGYILWGTRLSLVQVVAVLPFVSLAALGARGMGLP
                     TLILVLPIGAMAAVSLIFPAFYPRWFSFVFPFYAMLLTSGVLRIAPRRAVAAVLVAAV
                     IACNVPVLDRYYLDPSFRPWNWRGAATYVRGHFQPHDLLIFIGDNAMLPFTYYFSGDV
                     SLTVTPMEATGRSANFFSYRVREIAAQHPRLWVMSTVVWQPQLTQRLIPMLLTSYHMV
                     RAERFSGADVYLFEVSPAAGARQEAMP"
     gene            complement(2100..>2440)
                     /locus_tag="VEW91_03900"
     CDS             complement(2100..>2440)
                     /locus_tag="VEW91_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYM90763.1"
                     /translation="FDPREHPLNPDRSNDDKACAVFGHYCPVFFVNEPFTETREHRRV
                     GRHIPREIMLRVVRRDNNQCQTCGRILRDDEIEFDHIIPLALGGSSEEHNIRVACFDC
                     NREKSALYEP"
BASE COUNT          484 a          798 c          763 g          395 t
ORIGIN      
        1 acctgaccgt ctcccaagtg ttatgcgtgg cggatcgtag cgcaggtcgt gctccttcac
       61 gatctgggcc ccgcggccct acggcatcgc ctcctgacgc gccccagcgg cgggactcac
      121 ttcgaacagg tacacgtccg cgcccgaaaa ccgctccgcg cggaccatat ggtacgaagt
      181 caacagcatc gggataagcc gctgcgtcaa ctgcggttgc cagacgaccg tcgacatcac
      241 ccacaggcgc gggtgttgcg ccgctatctc ccggactcgg tacgagaaga agtttgcgct
      301 gcggcccgtc gcttccatcg gcgtcacggt cagcgacacg tcgccgctga agtagtaagt
      361 aaacggtagc attgcgttgt cgccgatgaa tatcagcaga tcgtgcggct ggaagtgccc
      421 ccgcacgtac gttgccgcgc cgcgccaatt ccacggccgg aaggaggggt cgagatagta
      481 cctgtcgagc accggcacgt tgcatgctat gaccgcggcg actaggaccg ccgccaccgc
      541 ccggcgcggg gcgattcgca ggacgccgct ggtcagcagc atcgcgtaga aggggaacac
      601 gaacgagaac caccgcgggt agaaggcggg gaagatcagg gaaacggccg ccatcgcgcc
      661 gatgggcaga acgagaatca gcgtcggaag gcccatgcca cgggctccta gagcggccag
      721 cgagacaaac gggaggacgg ccacgacttg cacaaggctc agtcgcgtcc cccagaggat
      781 gtaccctccc agaccaaaga gcgagccccc gaacgcgaag agcccgccga cgtcgagcag
      841 ggggtggatc catccccata cgtgccgttt cacaacctga tcgaagaagt agggcatcca
      901 ggggatgaac aggatgccag cgacgccagc gccgatcgcc cactccatca gccgccgacg
      961 atcgcgccac gcggtccaga tcccgtgcgc ggcgagcacg acgaagccga gataatgcgt
     1021 gtacatcatc gcggccgcgg tgccgacata ggccgtgcta tgccacaagc ggcgggtcgg
     1081 aaggatcaga agcaacgtcg atgccagagt caacatctcc agaagcgtgt acatccgggc
     1141 gttctgcccg gccattacct cgaacggcga gacggcgacg aacaacgcgg cgatccgggc
     1201 tgccctgtcg cctgacaacc gccgcatgat cgcgtacgtc agcgggacgg agacgactcc
     1261 gaacatcgcc gaaggcgccc tcagcgcggc ctcgccggtc cccgcgatgc ccgtccatgc
     1321 cttgattagc gcgaagtaca gcggcgggtg gttgtcatgg gccgccagga acacgatcag
     1381 atcgtgccag cccttctgga tagcatgggc gacgaacgcc tcgtcgtccc acaagctgtt
     1441 gtgcccgagg agcgcgaacc ggagggctgc cgcgattact atgataaggg taagtcccgt
     1501 atatcgcctc atcttcgtac ctgaacaata cccaagtccg gcctgcgctg gactcactcg
     1561 cacacaacga ccgcgcagag tgccgccaag ccggcgcacc gaatctctaa ccacgtcatg
     1621 tcatccgtca tggccgctca ccgtccgcaa gttatccaac gggctggccc tcctgaactt
     1681 ccggctaaca tccggcttgg tcagtggggg ggaccgcggg gccatttggt tccccacaag
     1741 cgggatggcc cgaaaggagt tcctttgggc cgggccccgg cggcgggtat gagggctctc
     1801 gggacgctgc atttacacgg cctggcggag taggctagga agccaccctt tcggccgcca
     1861 gcctccggtc gagcgtgctg cggctcacgc ccaaaagggg cgccgcctag cggcggctca
     1921 gggccctcga tgcaacgcgg ggcgcgagct cgcgcaatcg ctctccgaac ccgtcccgct
     1981 gcgggccgcc cggccggccc acgtgccggc cctgccggcg cgcccggtcc atcccggcgc
     2041 gcacgcgctc ggccaggatc ccgcgttcga gctgcgcgta cgcggccgcg atgtaggcgt
     2101 cacggctcgt acagagcaga tttctctcga ttgcaatcaa aacacgctac gcggatattg
     2161 tgttcttcgg agctgccgcc cagggccagt ggaatgatgt gatcgaattc aatttcatca
     2221 tctcgtagga ttctgccaca ggtttgacac tgattgttat ctcggcgaac gacgcgcagc
     2281 attatttctc gaggaatgtg ccggccgaca cggcgatgct ctcttgtttc ggtaaacggc
     2341 tcgttcacaa aaaatacggg gcaatagtgt ccgaacacag cgcatgcctt gtcgtcgttt
     2401 gatcgatccg gattcaacgg gtgttcgcgg ggatcgaacg
//
LOCUS       DASQGH010000205         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3733, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000205 DASQGH010000000
VERSION     DASQGH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..793
                     /locus_tag="VEW91_03905"
     CDS             116..793
                     /locus_tag="VEW91_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYM90764.1"
                     /translation="MADIGANVARVRARIAEAALRRGRRPDSVLLVAVTKTVGLDRIR
                     EGVACGLRVLGENRVQEARTKVPEIPGVVWHLVGSLQRNKVKEALRLFETIHSVDSLG
                     LAEEISRRAAEELARPAEVLIQVNISAEAQKHGVPPEAAEEVVAGARRLPGIRVRGLM
                     GMAPLVSDPEAARPCFRRLRELRDRLREALAAATLTDLSMGMTDDFEVAIEEGATMVR
                     IGRALFA"
     gene            868..1269
                     /locus_tag="VEW91_03910"
     CDS             868..1269
                     /locus_tag="VEW91_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HYM90765.1"
                     /translation="MGRIMQRMWNFLGFEDDQPSGPSRDAPGARAPVFSLHTQRTMEI
                     VVLEPAAYDETQTAADYLKTHRPIVVNLRSAQSDLGRRVVDFLSGVTYALDGHMQRVG
                     EEIFLFTPRQVFITAEQIREETRSGSLFPID"
     gene            1327..2379
                     /gene="mtnA"
                     /locus_tag="VEW91_03915"
     CDS             1327..2379
                     /gene="mtnA"
                     /locus_tag="VEW91_03915"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HYM90766.1"
                     /translation="MSVDAPLWWEDGALCLLDQTCLPHEVRTVRCTTWEEVAEAIRAL
                     RVRGAPAIGLAAAYGLVLAAGSDARRPSDRPQDTLRRAATGLRATRPTAVNLAWAIDR
                     MLRIAEEGSPDPADRARRLLEAADALARADVEANRRIGEHGAALIPAGARVLTYCNTG
                     MLATAGYGTAFGVLRRAHEEGRGIHVIACETRPVLQGARLTAWELLRAGIPATLITDN
                     AAGALMRAAGVDTVVVGADRIAANGDVANKVGTYTLAVLARTHGIPFYVAAPLSTVDL
                     ATPSGAEIPIEERPPGEVVMLGGVRVAPQEIRVRNPAFDITPHELVTAIVTDAGVVRP
                     PYGPGLRRILPKNTIF"
BASE COUNT          429 a          887 c          905 g          395 t
ORIGIN      
        1 gcggatggcg gcctatatca gcctccaccc ctagggcaag gacctcgttc cccctccgtc
       61 gaatacactc ggcccctgac gcccccgcta cccggaggcg cgggggcagg ccgcaatggc
      121 ggatatcgga gccaacgtcg cgcgggtgcg cgccaggatc gcggaggcgg cgctgcgccg
      181 ggggcggcgt cccgacagcg tgctgctcgt cgcggtcacg aagacggtcg ggctcgatcg
      241 tatccgtgag ggcgtggcgt gtggcctgcg cgtgctcggg gagaaccggg tgcaggaggc
      301 gcggaccaag gttccggaga tccccggagt ggtgtggcat ctcgtgggga gcctgcagcg
      361 gaacaaggtg aaggaggcgc tccggctgtt tgaaacgatc cactccgtgg attccctcgg
      421 ccttgccgag gagatcagcc gccgggcagc ggaggagttg gcccggccgg ccgaggtgct
      481 tatccaggtg aatatctctg cggaggccca gaagcacggc gttccgccgg aagccgccga
      541 ggaggttgtc gccggagcgc ggcggctgcc ggggatccgg gtgcgcgggc tcatggggat
      601 ggcccccctc gtttccgacc ccgaggcggc ccgcccgtgc ttccggcgcc tgcgggagct
      661 ccgcgaccgc ctccgcgagg cactggctgc ggccacgctc accgacctct cgatgggcat
      721 gacggacgat ttcgaggtgg cgatcgaaga aggagcaacg atggtccgca tcggccgggc
      781 gctgttcgcg tagacgacca cacagaccca gaacgacatc gtagcgcggt agccgcacga
      841 gacgcagcat cgacggaggg agcaatcatg ggccggatca tgcagcggat gtggaacttc
      901 ctcggcttcg aagacgatca gccgtccggg ccgtcccgcg acgccccggg cgcgagagcc
      961 ccggtgttca gcctccacac ccagcgcacc atggagatcg tcgtgctcga gccggcggcg
     1021 tacgatgaga cccagaccgc ggccgactac ttgaagaccc acaggccgat cgtcgtgaac
     1081 ctgcggtccg cccagagcga tctcgggagg cgcgtcgtcg acttcctcag cggggtgacg
     1141 tacgccctgg atgggcacat gcagcgggtc ggggaagaga tcttcctctt caccccgcgc
     1201 caggtgttca tcaccgccga gcagatccga gaggaaaccc gatcggggtc cttgtttccc
     1261 atcgattgac gcacccgggc tccggcatcc gtcccggcgg cggtggccgg ctcccggagc
     1321 gtgccggtga gcgttgacgc tcccctctgg tgggaagacg gcgcgctctg cctcctcgat
     1381 cagacctgcc tgccccatga ggttcggacg gtgcgctgca ccacgtggga ggaggtggcc
     1441 gaggcgatcc gggcgctgcg ggtgcggggg gcccccgcaa tcggcctcgc cgccgcctac
     1501 gggctggtgc tggcggccgg atcggacgcg cgccgcccgt ccgatcgccc gcaggacacg
     1561 ctgcggcggg cggccaccgg tctgcgcgcg acccggccca ccgccgtcaa cctcgcctgg
     1621 gcgatcgatc gcatgctgcg cattgccgaa gaagggtccc cagaccccgc ggaccgcgcc
     1681 cggcggctcc tcgaggccgc ggacgcgctt gcgcgggccg atgtcgaggc caaccgtcgg
     1741 atcggcgagc acggggcggc gctgatcccg gccggcgccc gggtgctcac ctactgcaac
     1801 accgggatgc tggccacggc cggatacggg acggcgtttg gtgtcctccg gcgcgcgcac
     1861 gaggagggcc gtgggatcca cgtcatcgcc tgcgaaacgc gcccggtcct ccagggcgcc
     1921 cggctcactg cgtgggaact cctccgcgcc ggcatccccg caacgctgat caccgacaac
     1981 gcggccggcg ccctcatgcg cgccgccggc gtggacacgg tcgtcgtggg ggcggaccgg
     2041 atcgccgcca acggcgacgt ggcgaacaag gtcggcacct acacgctcgc cgttctcgcc
     2101 cgcacgcacg ggatcccctt ctacgtggcc gcgccgttga gcaccgtgga tctcgccacc
     2161 ccatcggggg cagagatccc gatcgaagag cggcctcccg gggaagtcgt catgctcggg
     2221 ggcgtccggg tcgccccgca agagatccgg gtccgcaacc ccgccttcga catcaccccc
     2281 cacgagctgg tcacggcgat cgtcaccgat gccggggtcg tgcgtccccc gtacgggccg
     2341 ggtcttcggc ggattctacc caaaaacacc atattttagt gagtttctag agattcaggg
     2401 atagcggaat cttcgagccg ttagcaagaa tcgcgactta tccacgatat ccacaacata
     2461 cgaacagctg tttgtgccat attaaatacc ccattatgta aggtcttgag gtgttccggc
     2521 tcttgagtct ccctcatggg catcaatagt tttcagaaac aaatctgctg gcacaaaatc
     2581 aagcgcggaa agaagggagg tgagagagat gaaacg
//
LOCUS       DASQGH010000206         7471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_34869, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000206 DASQGH010000000
VERSION     DASQGH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34869"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VEW91_03920"
     CDS             <1..838
                     /locus_tag="VEW91_03920"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HYM90767.1"
                     /translation="FEDGEIYARFEESVRGNDTFIIQPTCPPVNENLMELLVMVDALR
                     RASAGRITAVMPYYGYARQDKKSAPREPITGRLVADLLQSAGVHRILALDLEADQIEG
                     FFNIPVDHLRALPLFAEYLKEKTLEPAVVVAPDDGAVKIAYRLAERLHLPLAVVFQRR
                     PGPDTRQAAQVVGEVEGRIPILIDRIVSTGGRIATALDALMQQGARPGAFVCATHAVF
                     SGPIGQALGRGDVQEFAFTNTIPISPEVMTDKIRILSAAPLLARAMRNIHLNESVSTL
                     FT"
     gene            918..2411
                     /gene="gltX"
                     /locus_tag="VEW91_03925"
     CDS             918..2411
                     /gene="gltX"
                     /locus_tag="VEW91_03925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYM90768.1"
                     /translation="MTVRTRYAPSPTGYLHVGGAWMAFFNWLFARHHKGAFVLRIEDT
                     DRDRSTEEFERSILEDLRWLGIDWDEGPDKGGPCGPYRQTERGAMYRAHAQTLLEREA
                     AYPCYCTREELEADRQQATAEHRPPRYSGRCRTLSPGQRRAQEAAGRVATLRFRVPPG
                     HPTIGVEDLVLGRVEFDPAALDDFIIQRSDGTPLYNFANVVDDHGMQISHIFRGSEHL
                     SNTPRQVLMYEAFEWTPPRFAHFPLLLGVDRKKLSKRHGDTAVREYAAQGFLPEALVN
                     FFALMGWYPQDGRELFTVQELITRFRIEEVGKSAAVFDIQKLNWMNGVYMQAAYRQSP
                     DRVVDLVIACLRDQGLLDGEVTPERRAYVSRVVSIMGERLRVPRDILTYGDFFFRPQA
                     TFDPQAVARHLSVPGSAQLLRHLRDALQATRPWDLASIEGAVRGAAAHLGVEDKAVIH
                     PLRVALTGKTVGPGLFELIEVLGKDRVLDRLDRAIARLPEPPASAPR"
     gene            2408..3133
                     /locus_tag="VEW91_03930"
     CDS             2408..3133
                     /locus_tag="VEW91_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYM90769.1"
                     /translation="MTARFVVGTSGWNYPHWRGRFYPRELAQREWLGYYARHFPTVEI
                     NNTFYRLPLSQTFAQWRSVVPSSFTFAVKASRYITHIKRLRLSRAPVRRLLRRARRLG
                     RGLGPILFQLPPNFAPDEPRLLRFLTGLPTRYRYAMEFRHAAWHRDSVYGLLRRRGVA
                     CCISDGPGIPLGVVRTAAFVYVRFHGPDGIGAGRYPTATLRRWAQTLRDLCGNRTVYV
                     YFNNDEAGYALENARQFIDLLRE"
     gene            3451..3524
                     /locus_tag="VEW91_03935"
     tRNA            3451..3524
                     /locus_tag="VEW91_03935"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3483..3485,aa:Gln,seq:ctg)
     gene            3580..4737
                     /locus_tag="VEW91_03940"
     CDS             3580..4737
                     /locus_tag="VEW91_03940"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HYM90770.1"
                     /translation="MTYDLLLTGGTLVDPAQGIHAPRDVAFAGGRVAAVAGDLPRADA
                     AHVIDCAGQIVCPGMIDLHVHVFWGVSHYGIEPDPHCIAKGVTTAVDAGSAGADTFPG
                     FRKYVIDVSATRLLAHLNISSQGMLSRDVGELDDIKYANVPKAIATIERHRDLILGVK
                     VRLTRHEIVGASAGIRPLHLAREAADAVGLPIMVHPQEAWCDSLDDILAVMRRRDILT
                     HCYHGLSHGILDADGNVRRSVRAAMERGVIFDVGHGAGSFSWDVAERALRQGVQPQTI
                     SSDLHAYNVNGPVYDLATTVSKFLCLGVSLDEALRRVTATPAEALRMAGEIGTLQVGA
                     WGDAVVFDLEEGRFEFGDSHGQVRLGQRRLVPRQVVRAGKVYRPPEDIGPH"
     gene            4748..5764
                     /locus_tag="VEW91_03945"
     CDS             4748..5764
                     /locus_tag="VEW91_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020006937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90771.1"
                     /translation="MVTLYVATGDALVAAGRPNGRWQADLQLVGVPTQCIASDPLGGP
                     DLYCGTFGQGLWVSHDAGASWHPAGEGIPHRDIMAVAVSPRERRGDAGVVWAGTEPSA
                     LFRSEDGGRTWQERPALRALPSAPTWSFPPRPWTHHVRWIAPDPLVDGRLFVAIELGG
                     VMRTLDGGMTWEDRKPGSQADAHTLGTHPLAHGRVYEAAGGGYAESRSGGATWRSDDE
                     GLQHSYLWGLAVDPGDPETVVVSAARGPFEAHHPDDAWSTVYRRSRNRRWQEVVDGLP
                     PQRGRRVVTLASHPGEPGVFYAAVQEGDLYRSADGGCTWERLDVAWPGGYRYRHVHGL
                     AAVA"
     gene            complement(5788..6924)
                     /gene="solA"
                     /locus_tag="VEW91_03950"
     CDS             complement(5788..6924)
                     /gene="solA"
                     /locus_tag="VEW91_03950"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HYM90772.1"
                     /translation="MASYDVIVLGCGGMGSATAYHLAQRGVRVLGLEQFGVAHDQGAS
                     HGRTRVIRQAYYEGPDYVPLLFRAYDNWHALESLAGAPLFTKTGALHLGAADSAGVAG
                     AALSARTHRIPHEMLTAEEIRKRYPVLRPRPDQVALLEYEAGFLLPERCVAAYIELAR
                     RRGADLHFEERVDSWTATPGRVVVRTARATYEADRLVITAGPWAGQVLRDLGLPLEVT
                     RAVVYWFEPRAETKAFRKIPVYLWEDHGIYAYGFPYLDGQGLKCGFHHVHYEVTTPQT
                     IRRQVGEDEKVRMRDHLAELMPDAAGEVLSFSTCMYTNTPDHHFIIDRHPGHDNVVLG
                     CGFSGHGFKFASVVGEVLADLALQGKTALPISLFALRRFTPPVR"
     gene            7042..7117
                     /locus_tag="VEW91_03955"
     tRNA            7042..7117
                     /locus_tag="VEW91_03955"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7076..7078,aa:Glu,seq:ctc)
BASE COUNT         1140 a         2551 c         2578 g         1202 t
ORIGIN      
        1 gttcgaagat ggggagatct acgcgcggtt tgaggaaagc gtccgaggca atgacacctt
       61 tatcatccag cccacctgtc ctcccgtcaa cgagaacctg atggagttgc tcgtcatggt
      121 ggacgccctg cgccgggcgt cggcggggcg gatcacggcg gtgatgccgt actacgggta
      181 cgcgcggcaa gacaagaaga gcgccccgcg ggagccgatc accggacggc tggtggccga
      241 cctgctccag agcgcggggg tgcaccggat cctggcgctg gatctggaag ccgatcagat
      301 cgaagggttc ttcaacatcc cggtggatca cctccgggcg ctcccgctgt tcgccgagta
      361 cctgaaggag aagacgctcg agcctgcggt cgtcgtcgcc cccgacgatg gcgccgtcaa
      421 gatcgcctac cggctggccg agcggctgca cctgccgctg gccgtcgtct tccaacggcg
      481 gcccggtccg gacacccggc aggcggcgca ggtcgtcggc gaggtggagg gccggatccc
      541 gatcctcatc gaccgcatcg tgtcgacggg gggcaggatt gccaccgccc tggacgcgtt
      601 gatgcagcag ggagcccgcc ccggggcgtt tgtctgcgcc acgcacgccg tgttctccgg
      661 ccccatcggg caggccctcg gtcggggtga tgtccaggag tttgcgttta ccaacaccat
      721 cccgatctcc ccagaggtca tgaccgacaa gatccggatc ctttcggccg ccccgctcct
      781 cgcccgcgcg atgcgcaaca ttcacctcaa cgagtcggtg agcacgctct tcacctaggc
      841 ggcgccgccg gccccaagcg cgcccgccca cggccacgcg tttgcagccg cgccgggccg
      901 tgtgctaccc tggcaccatg acggtccgga ctcgatacgc acccagtccc accggctacc
      961 tccatgtggg aggagcctgg atggcgtttt tcaactggct gttcgcccgc caccacaaag
     1021 gcgcgtttgt cctccggatc gaggacactg atcgggaccg gtcgaccgag gagttcgagc
     1081 ggtcgatcct cgaggacctg cgctggctgg ggatcgactg ggatgagggt ccggacaagg
     1141 gcggcccctg cggcccgtac cggcagaccg agcggggcgc tatgtaccgc gcgcacgcgc
     1201 agacgctgct cgagcgcgag gcggcctacc cgtgctactg cacgcgcgag gaactcgagg
     1261 ccgatcgcca gcaggccacc gccgaacacc ggcccccccg ctattcgggc cgatgccgga
     1321 ccctgtcgcc cggacagcgg cgcgcccagg aggcggccgg ccgggtcgcc accctccggt
     1381 tccgggtccc cccgggccac ccgacgatcg gggtcgagga cctcgtcctc gggcgcgtgg
     1441 agttcgatcc ggcggccctg gacgatttta tcatccagcg gtccgacggg actcccctgt
     1501 acaacttcgc aaacgtcgtg gacgaccacg ggatgcagat ctcgcacatc ttccggggtt
     1561 cggagcacct ctcgaacacg ccccgccagg tcttgatgta cgaggccttc gagtggaccc
     1621 cgccccggtt cgcgcacttc cccctgctgc tcggggtcga tcggaagaag ctcagcaagc
     1681 ggcacggcga caccgcggtt cgggagtacg ccgcgcaggg gttcttgccc gaggcgctgg
     1741 tcaacttctt cgccctgatg gggtggtacc ctcaggacgg ccgggagctc ttcaccgtcc
     1801 aggagctgat cacccggttc cggatcgaag aggtggggaa gagcgccgcc gtcttcgaca
     1861 ttcagaagct gaactggatg aacggcgtct acatgcaggc ggcgtaccgc cagagcccgg
     1921 accgcgtcgt cgacctcgtc atcgcctgcc tccgggacca gggcctcctc gacggcgagg
     1981 tcacgcccga gcggcgggcg tacgtgagcc gtgtggtctc catcatgggg gagcggttgc
     2041 gggtgccgcg cgatatcctc acgtacggcg actttttctt tcgtccccag gccaccttcg
     2101 acccgcaggc ggtcgcgagg cacctgagcg tcccgggcag cgcgcaattg ctgcgccatc
     2161 tcagggacgc gctacaagcg acgcgcccgt gggaccttgc gtcgatcgaa ggggcggtcc
     2221 ggggcgctgc ggcgcacctc ggggtcgagg acaaggcggt catccatccc ctgcgggtcg
     2281 cgctgaccgg caagaccgtc ggcccggggt tgttcgagct gatcgaggtg ctggggaagg
     2341 accgcgtgct tgaccggttg gaccgggcga tcgcccggct gcccgagccg cccgcgtccg
     2401 ccccccggtg accgcgcgat tcgtcgtcgg cacgtccggc tggaactatc cgcactggcg
     2461 cggccgtttc tatccccgcg agcttgctca gcgggagtgg ctcgggtatt atgcccgcca
     2521 cttcccgacg gtcgagatca ataacacgtt ctaccggctt ccgctttccc agacgtttgc
     2581 gcagtggcgc agcgtggtgc ccagctcatt cacgtttgcg gtcaaggcca gccggtacat
     2641 cacccacatc aagcggctgc ggctgagccg tgctccggtt cgacggctcc tgcgccgcgc
     2701 ccgacgtctc ggccgcgggc tcggccccat cctgtttcag ctgccgccca acttcgcccc
     2761 cgacgagccg cgcctcctgc gcttcctcac cggattgccg accaggtacc ggtacgcgat
     2821 ggagtttcgc cacgccgcct ggcaccggga ttccgtgtac ggtctcctgc gacggcgcgg
     2881 cgtcgcgtgc tgtatcagcg atgggccggg tattccactc ggggtcgtgc ggaccgccgc
     2941 gtttgtctac gtgcgcttcc acggccccga cggcatcggc gcgggccggt atccgaccgc
     3001 gacgctcagg cggtgggcgc agacgctgcg cgatctgtgc ggaaacagga ccgtctacgt
     3061 gtacttcaac aatgatgagg ccggctacgc gctggagaac gcgaggcagt tcattgatct
     3121 actgagggaa taacccgccc gacccctggg ttcgtccatt ccacatggcc gcaggaaaat
     3181 gacagccagt catcgggatc ccggtcctcg tacaggaaca tcgggtgcat ctcacccttg
     3241 cacctgggac agggcaacgt gccgtcccga cgctcgtccg gaggatgcgg cttgggcatt
     3301 agacctcctg ctccgggact tcccggtgat tccatggtag cataggtcgc ggccgctcaa
     3361 caccccgccc ccctctcgaa gccttgggcg tgctgttcgc gttgacctcg gcgcgggggg
     3421 cccagtataa tgagaacgct tcgggcaccc tgggggatcg tctagcggta ggacgacggg
     3481 ctctggaccc gttagcgggg gttcgaatcc tcctccccca gccacacaag ctgctgggat
     3541 tgccttcccg ctgtctcatc ggggaggggc cgtcgcggaa tgacgtacga cctgctactg
     3601 acgggcggaa ccctggtcga tcccgcgcag ggcatccacg ccccgcgaga tgtcgcgttt
     3661 gccgggggca gggtcgcggc tgtcgccggg gacctgcccc gcgcggacgc cgcccacgtc
     3721 atcgactgcg ccgggcagat cgtctgcccg gggatgatcg acctccatgt ccatgttttc
     3781 tggggggtca gccactacgg gatcgagccc gatccccatt gcatcgcgaa gggggtcacc
     3841 accgcggtgg acgcggggtc ggcgggggcg gataccttcc cggggttccg gaagtacgtg
     3901 atcgacgtca gcgcgacccg tctgctcgcg cacctcaaca tctcgtccca gggcatgctg
     3961 agccgtgacg tgggcgagct ggacgacatc aagtacgcca acgtccccaa ggcgatcgcc
     4021 acgatcgagc gtcaccgcga tctgatcctg ggggtgaagg tgcggctcac ccgccacgag
     4081 atcgtggggg cgtccgccgg gatccgcccg ctccacctgg cgcgggaagc cgccgacgcc
     4141 gtcggcctcc cgatcatggt gcacccgcag gaggcctggt gcgactcgct cgacgacatc
     4201 ctggccgtga tgcgccgccg cgacatcttg acccactgct atcacggcct gtcgcacggc
     4261 atcctggacg ccgacggaaa cgttcggcgg tcggtgcggg cggcgatgga gcgcggggtg
     4321 atctttgatg tcgggcacgg ggccgggagc ttcagctggg acgtcgccga gcgggcgctg
     4381 cggcagggcg tccagccgca gacgatctcg agcgacctcc atgcgtacaa tgtcaatggc
     4441 ccggtgtacg atctcgcgac gaccgtcagc aagttcctgt gcctcggcgt ctcgctcgac
     4501 gaggcgctcc ggagggtgac ggcgacgccg gcggaggcgc tccggatggc gggcgagatc
     4561 gggaccttgc aggtgggcgc gtggggcgac gcggtcgttt tcgacctcga ggaaggccgg
     4621 ttcgagttcg gggacagtca cgggcaggtg cgccttgggc agcgccggct cgtccccagg
     4681 caggtggtgc gggcgggcaa ggtgtatcgc ccccccgaag acatcgggcc gcactaggga
     4741 ggacgcgatg gtcacgttgt acgtcgccac cggcgacgcg ctggtcgcgg ccggcaggcc
     4801 gaacgggcgc tggcaggcag accttcaact ggtcggggtg ccgacccagt gtatcgcctc
     4861 cgatcccctg ggcggcccgg atctctactg cgggaccttc ggccagggcc tgtgggtcag
     4921 ccacgatgcg ggagcgtcct ggcacccggc cggcgagggc atcccccaca gggacatcat
     4981 ggccgtggcg gtgagcccgc gggaacggcg cggggacgcc ggggtggtct gggcgggaac
     5041 cgagccgagc gcgctgtttc gctccgagga cggagggcgc acgtggcagg agcggcctgc
     5101 gctgcgagcg ctcccatcgg caccgacgtg gagctttccc ccccgcccat ggacgcacca
     5161 tgtccggtgg atcgcccccg accccctggt ggacggccgc ctcttcgtcg cgatcgagct
     5221 gggcggcgtg atgcggaccc tggacggcgg gatgacgtgg gaggatcgca agcccggctc
     5281 ccaggccgac gcccacaccc tggggacaca cccgctggcc cacggccggg tctacgaggc
     5341 cgcgggcgga gggtacgcgg agagccgcag cgggggagcc acctggaggt cggatgacga
     5401 gggcctccag cactcgtacc tgtggggcct ggcggtcgat cccggggacc cggagaccgt
     5461 ggtggtctcg gcggcacgcg gtcccttcga agcccaccat ccggacgacg cctggtccac
     5521 ggtgtaccgc cggtccagga accgccggtg gcaggaggtc gtggacggcc tgccccccca
     5581 gcgcggccgt cgggtcgtga ctttggcgtc gcatccggga gagcccggcg tcttctacgc
     5641 cgcggtgcag gaaggcgatc tgtatcgctc agccgatggc ggctgcacct gggagcggct
     5701 cgacgtcgcc tggcccggcg gatatcggta ccgccacgtc cacgggctcg ccgcggtcgc
     5761 ctgaccgggc gaaggcggcg ggcgtgttca gcgcaccggt ggagtgaacc ggcggagcgc
     5821 gaagaggctg atcggcagcg cggtcttccc ctggagcgcg aggtcggcca ggacctcgcc
     5881 gacgacgctg gcaaacttga agccatgccc cgagaagccg caccccagga ccacgttgtc
     5941 gtggcccgga tgacggtcga tgatgaagtg gtggtccggg gtattcgtgt acatgcacgt
     6001 ggagaatgac aggacttcgc cggccgcatc gggcattagc tctgccagat ggtcgcgcat
     6061 gcgcaccttc tcgtcctcgc cgacctggcg gcggatggtc tgtggggtgg tgacctcata
     6121 gtgcacgtgg tggaacccgc acttgaggcc ctgcccatcg aggtagggga agccatacgc
     6181 gtagatgccg tggtcctccc acaggtagac cggtatcttc ctgaacgcct tggtctcggc
     6241 gcgcggctcg aaccagtaga ccaccgcgcg cgtgacctcg aggggcaggc cgaggtcgcg
     6301 aaggacctgg ccggcccacg ggcccgcggt gatcacgagg cgatcggcct cgtaggtggc
     6361 ccgggcggtg cggacgacca cccggccggg ggtcgcggtc caggagtcca cccgctcctc
     6421 gaagtggagg tcggcccccc ggcgcctggc caactcgata taggcggcga cgcaccgctc
     6481 ggggaggagg aagcctgcct cgtactcaag gagggctacc tgatccggcc gcggccggag
     6541 gaccgggtag cgcttccgga tttcctcggc ggtgagcatc tcatggggaa tccgatgcgt
     6601 gcgggcactg agcgccgccc cggccacgcc cgccgaatcg gcggcgccga ggtggagcgc
     6661 gcccgtcttc gtgaagaggg gggcgccggc cagggactcc agcgcgtgcc agttgtcgta
     6721 ggccctgaac agcagcggaa cgtagtcggg cccttcgtag tacgcttggc ggatgacgcg
     6781 ggtccgtccg tgcgacgcgc cctggtcgtg cgccaccccg aactgctcga ggccgagcac
     6841 acgcaccccc cgctgcgcaa ggtgataggc ggtggcgcta cccatgcccc cgcaccccaa
     6901 cacgatgaca tcgtagctcg ccatcgaggc tcctcttttc cacgatctcg cacccgtccc
     6961 ctgtcccggc cccccctcgg ggtggtacgc cgcggccgac gtctggtata ctcgttgagg
     7021 aaccgcaccc cggttccacg cggccccatc gtctagaggc ctaggacgcg gccctctcaa
     7081 ggccgaaacg gggattcgaa ttcccctggg gccaccaaaa ggaataccgc catatttcat
     7141 gcggtgttct aagcataagg cccctcaatt aatcccccac ccatcgccgt gccttaggca
     7201 tggatgccta tcgaaagggg gccaactgcc acgacatgaa gaccgcgacc cagaccctgg
     7261 atggcgttgc tgtcaggcgc ccccgaccca ccccgacgca tcctcagcat ctctgcttgg
     7321 aggttgtgga acggactggc tcgtggcaca atcggttccg caaattactc atccgcttcg
     7381 agaaacggat gagcaactat ctaggcttgc tggggcctat cggtgaagcc tgttgttggc
     7441 cattgcgtta tgtgcaccgc gggggcctgc g
//
LOCUS       DASQGH010000207         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23580, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000207 DASQGH010000000
VERSION     DASQGH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23580"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /gene="vorB"
                     /locus_tag="VEW91_03960"
     CDS             <1..855
                     /gene="vorB"
                     /locus_tag="VEW91_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HYM90773.1"
                     /translation="GAGVRVMTSTSGPGISLMQEGLSYLVGAELPCVVVDMMRGGPGL
                     GNIAPAQGDYFQATRSAGHGDGRLLVLAPSTVQEACDLTLLAFELADRYRNPVMILGD
                     GVLGQMMEPVTLPDPVEPARDVTWATTGAGEGPRRIVTSIHLEPEVLEAHNLRLQEKY
                     RRAQREARWEAYRIEDAVLAVVAYGIVARVARTGVDRARDKGLRVGLLRPITLFPFPV
                     QPVRELPHIKAFLVTELSAGQMVEDVRLAVEGRAPVRFYGRLGGMMLTPPEVLAQIEK
                     VAAEVGGQ"
     gene            852..1601
                     /locus_tag="VEW91_03965"
     CDS             852..1601
                     /locus_tag="VEW91_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM90774.1"
                     /translation="MKQVFARPATLLETPMHYCPGCTHGIAHRLVAEVIDELGLRGRT
                     IGVASVGCSVFAYNYLDVDFQQAAHGRAPAVATGIKRVCPDRVVFTYQGDGDLAAIGT
                     AEIIHAANRGENITVIFINNAVYGMTGGQMAPTSLLGMRTTTSPSGRDARLAGYPLRV
                     AELLAPLPGVAYLARVALSSPAHVFKAKKAIRKAFQTQVGGRGFSLVEALSTCPVCWG
                     MTPVEALHWLDASMMPVFPLGEFKTPPGIGA"
BASE COUNT          252 a          523 c          595 g          290 t
ORIGIN      
        1 ggcgccgggg tgcgggtgat gacgtcgacg agtggtcccg ggatcagcct gatgcaggag
       61 ggactgtcct atctcgttgg cgctgaactc ccctgcgtcg tggtggacat gatgcgcggg
      121 gggccgggcc tcggcaacat cgccccggca cagggggatt acttccaggc gacccggtct
      181 gcggggcacg gcgacgggcg gctgctcgtc ctggccccgt cgacggtcca ggaggcgtgc
      241 gaccttaccc tcctggcgtt cgagttggcc gaccgttacc gcaaccccgt gatgatcctc
      301 ggtgatgggg ttctcggcca gatgatggaa cccgtcaccc tcccggaccc ggttgagcct
      361 gcgcgcgacg tgacgtgggc caccaccggg gcgggggagg ggcctcgccg gatcgtcacc
      421 tccattcatc tcgagccgga ggtgctggag gcgcacaacc ttcggcttca ggagaaatac
      481 cgtcgtgccc aacgcgaagc gcggtgggag gcctaccgca tcgaagatgc ggtcctggcc
      541 gtcgtggctt atgggatcgt cgcgcgcgtg gcccggacgg gcgtggatcg cgcgcgagac
      601 aaggggctcc gggtcggact cctgcggccg atcacgctct ttccgtttcc cgttcaaccc
      661 gttcgtgagc taccgcacat caaggccttc ctcgtcacgg agctgagcgc gggtcagatg
      721 gtggaagacg tccggctcgc cgtggaaggt cgagccccgg tgaggttcta cggcaggctc
      781 ggggggatga tgctcacgcc cccggaggtc ctggcgcaga tcgagaaggt ggccgcggag
      841 gtagggggcc aatgaagcag gtctttgcgc gtcccgcgac gttgttggag accccgatgc
      901 actactgccc tggatgcacc catggcatcg cccaccgcct cgtcgcggag gtgatcgacg
      961 agctgggcct cagggggagg acgatcggcg tggcatcggt gggctgctcg gtgttcgcct
     1021 acaactacct cgacgtggat tttcagcagg ccgcgcacgg ccgggcacct gcggtggcca
     1081 cgggcatcaa gcgcgtctgc cccgataggg tggtgtttac ctatcaaggg gatggcgacc
     1141 tggcggcgat cgggacggcg gagatcatcc acgccgcgaa tcgcggagag aacatcaccg
     1201 tgatcttcat caacaacgcg gtctacggca tgaccggagg acagatggcg cctacgagcc
     1261 tgctgggcat gcggacgacg acgagcccgt cgggtcgcga cgctcgcctg gcaggctatc
     1321 cgcttcgggt cgccgagctg ctggcgccgc tgcccggcgt cgcctacctc gcccgcgtgg
     1381 cgctctccag cccggcccat gtcttcaagg cgaagaaggc catccgtaag gcgtttcaga
     1441 cccaggtggg gggccgagga ttctcgctcg tcgaggcgct ctcgacctgt ccggtatgct
     1501 gggggatgac accggtcgaa gccctgcact ggctggacgc cagcatgatg ccggtcttcc
     1561 ctttgggtga gttcaagacg ccccccggta tcggggccta ggccatggcg acgttcgaga
     1621 tcatcatcgc cgggttcgga gggcaggggg ttctcttcgt
//
LOCUS       DASQGH010000208         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40553, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000208 DASQGH010000000
VERSION     DASQGH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40553"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEW91_03970"
     CDS             <1..203
                     /locus_tag="VEW91_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYM90775.1"
                     /translation="LRRLPASTAAALQYLQPLVGVAASAALFGDRLGVWFLVGTTLVL
                     AGIALATAPSRREDRPPDTPNT"
     gene            235..1458
                     /locus_tag="VEW91_03975"
     CDS             235..1458
                     /locus_tag="VEW91_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HYM90776.1"
                     /translation="MHEEDYLQARADERRWALAKARGVSRRRLLQILAAGAAAAGAGR
                     LVRGSGAEGAPASDLLVKPTPPEFFIDFGSNKEMRWEVMYTRGYVVPNELFFVRDHSR
                     TPRVDPDTWRLRIEGTGVSRPQEFAYDEILRMPSVSVLRFIECAGNGRSFYEAAYGKR
                     AQGGQWKLGAIGVAEWTGVPLREVLDRAGLKRSARDVMPEGVDDLRVRRPMPIAKALM
                     DDTLLVYAMNGQILPPDHGFPLRVLVPGWVGVANTKWVGRIEVSEQPLYSPWNTDSYV
                     LIGPDYQPNPPAKGPVLSFQNVKSALELPWGAELRAGRQLIRGRSWSGQGKISKVVVS
                     ADNGVTWSTARLREPNIGQAWVRWDIEWDAKPGQYGLVARATDERGTTQPFQVPFNEQ
                     GYLYGAVVSHPVTVR"
     gene            1583..1888
                     /locus_tag="VEW91_03980"
     CDS             1583..1888
                     /locus_tag="VEW91_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYM90777.1"
                     /translation="MSHAAGVPVELSATEFRYEPKEVSTASGSVIFVVRNEGAIEHNF
                     VLEDAVGKKVAEIAVIEPGTTAEVTATLTPGAYTIACTLPGHRQAGMVATLKVAKSD"
     gene            complement(1885..3312)
                     /locus_tag="VEW91_03985"
     CDS             complement(1885..3312)
                     /locus_tag="VEW91_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90778.1"
                     /translation="MATSGAVHVEAVDPSIVDVREVEPGIIGIRGVRPGDTAIVVLTA
                     ADVLVWPVHVTPAPILPSTPVAGPGGVLDALYQPLPNPAIAGLIEPVCDDLGPGAAFR
                     PPWTCAQGPGANWAALFERGDVQGAFAAGSYNLDVHPRGGIQVVVASYLPTPLDPFGS
                     PPTWGIDTRAPWGTEVIDSPVGVEVLQHFRIANGDVFGGMTYLGPLAGIQLSFRPVEL
                     QVSTLASGGQVLAAGALGLRAGDVSVSYIASPIGNGIIVRLTQSPLDLAVSVGSSETD
                     VGVAYTFSGGQSVQGFWSSVNGFQVVYVAPLGEAVTRTTVVAGQPEFSATLEPTGGPQ
                     ITTVPVGPGSLTLTPVLLWGRTSSAHLRMPLAGPAAPEPLAPLAPAAPAGTLIVRACV
                     NSRRDDTCTQSDPALLITVLVDGTPVTMSGTGVSVPAGSHRVAVPLEAVPPLLVPLKG
                     LTCDLTIPAFGVGVCELPFRREGAP"
     gene            complement(3777..4514)
                     /locus_tag="VEW91_03990"
     CDS             complement(3777..4514)
                     /locus_tag="VEW91_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90779.1"
                     /translation="MGLVVVALLLLIAPAAAGPAAAPSSGGLAGQPVLQQVTVGPGVE
                     ADADLLVRNEDAQAALTVQLQIADLDPSGTVRPAATTPHTLTGSVTLPSQEIVLAPRE
                     TRKLAIRVRGDGHTRFGAIVVSAVTPGPNPAAFARIVSQLVVAAPGADLTPQTQLTVT
                     AQGLVQLQIQNPNAALCRGRGALFLLRSDGTFLGRLDVPEFALLPGGTTVLTLRWPAP
                     LGPGTVARVALAVDGASTPFIASSQVP"
BASE COUNT          742 a         1592 c         1669 g          645 t
ORIGIN      
        1 cgctgcgccg cctgccagcc agcacggctg cggcgctcca gtatctgcaa ccattggtgg
       61 gcgtggccgc ttccgccgcc ctcttcggcg accgtctcgg cgtgtggttt ttggtgggaa
      121 ccacgctcgt gctcgccggg atcgcgctgg ccacggcccc ctcccggcgc gaggatcgcc
      181 ccccggacac cccgaatacg tgaggcaacg ccgcaaccaa ggtggtgagc cttcatgcac
      241 gaggaggatt acctgcaggc gcgggccgat gaacgccggt gggcgctggc caaagcgcga
      301 ggggtgtccc gccgacgctt gctccagatc ctcgcggcgg gcgccgcggc ggccggggcc
      361 ggccggctcg tgcggggaag cggcgcggag ggggcgccgg caagcgacct cctcgtcaag
      421 ccgacgcccc cggagttctt catcgacttt ggcagcaaca aggagatgcg ctgggaggtc
      481 atgtataccc gcggctatgt ggtgcccaac gagctgttct tcgtccggga tcacagccgc
      541 accccgcgcg tagaccccga cacctggcga ttacgcatcg aggggaccgg ggtttcgcgg
      601 ccgcaggagt ttgcctacga cgagatcctc cggatgccct cggtgtcggt gctccgcttc
      661 atcgaatgcg ccggcaacgg tcggagcttc tatgaggccg cgtacgggaa gcgggctcag
      721 ggggggcagt ggaaactggg agcgatcggg gtggccgagt ggacgggcgt ccctctgcgg
      781 gaggtgctgg accgggccgg gctcaagcgc tcggcccgcg atgtcatgcc cgaaggcgtg
      841 gacgacctcc gggtgcggcg cccgatgccg atcgccaagg ccctgatgga cgacaccctg
      901 ctggtctacg ccatgaatgg gcagatcctg ccccccgacc acgggttccc actccgggtc
      961 ctcgtcccgg ggtgggtcgg ggtcgccaac accaagtggg tgggccggat cgaggtctcc
     1021 gaacagcccc tctactcgcc gtggaatacg gacagctacg tcctcatcgg accagactac
     1081 cagcccaacc ccccggccaa ggggccggtc ctgagcttcc agaacgtcaa gagcgccctc
     1141 gagctcccct ggggggcaga actgcgcgcg ggcaggcagc tcatccgggg gcggtcctgg
     1201 tccgggcagg gaaagatcag caaggtggtg gtcagcgcgg acaacggggt gacgtggtcg
     1261 accgcgcggc tgcgtgagcc caacatcggg caggcgtggg ttcggtggga catcgagtgg
     1321 gatgcgaagc cgggccagta cgggctcgtg gcccgcgcca cggacgagcg tgggacgacc
     1381 caacccttcc aggtcccgtt caatgagcag gggtatctct acggcgccgt ggtcagccat
     1441 ccggtgaccg tccgctgaac gccggcgctc gcagggggat cccctgacac ggaccatcgt
     1501 cccgggacgc cggcgggcgg cgcggctggc ccgcctcgcg attctcctct tcgccgccgg
     1561 ggcaggaccc tggatcgccc tcgtgagcca cgccgccgga gtgcccgtgg agctttcggc
     1621 gacagagttc cgctatgagc cgaaagaggt gtcgaccgct tcggggtcgg tgatcttcgt
     1681 tgtgcggaac gagggcgcaa tcgagcacaa tttcgttctg gaagacgccg tcggcaagaa
     1741 ggtcgccgag atcgccgtga tcgagcccgg gacgacggca gaggtcaccg ccaccctcac
     1801 cccaggcgcc tacaccatcg cctgcacgct cccgggccac cggcaagccg gaatggtcgc
     1861 cacgctgaag gtcgcaaagt cggactaggg ggccccctcg cgtcggaagg gcaactcgca
     1921 gacgcccacg ccaaacgccg ggatcgtgag atcgcacgtc agacctttga ggggcacgag
     1981 aagcgggggc acagcctcga gcgggacggc gaccctgtgc gacccggcgg ggacggatac
     2041 gcctgtgccc gacatcgtga cgggcgtccc gtccaccaag accgtgatca aaagcgcggg
     2101 atccgactgc gtgcaggtgt cgtcccgccg gctattcacg caggcccgaa cgatcagcgt
     2161 cccggccgga gcggcgggcg cgaggggcgc aaggggctcc ggcgcggcgg ggccggccag
     2221 gggcatccgc aggtgtgcgc tggaggttcg gccccagagc aggacggggg tcagggtcag
     2281 ggagccgggc cccaccggga cggtggtgat ctgcggcccc ccggttggtt ccagtgtggc
     2341 cgaaaattcg ggctgaccgg ccacgacggt cgtccgggtc accgcctccc cgaggggagc
     2401 cacgtacacc acctggaatc cgttcacgct cgaccagaac ccctggacgc tctggccgcc
     2461 cgagaacgtg taggccaccc ccacgtccgt ttccgagctg cccaccgaga ctgcgagatc
     2521 gagcgggctc tgggtgaggc ggacgatgat gccgttcccg atcggactcg cgatgtaaga
     2581 cacgctcacg tccccggcgc ggaggccaag cgctccggcg gccaacacct gcccgccgga
     2641 ggcgagggtc gaaacctgca gctcaaccgg ccggaaggaa agctggatgc cggcgagagg
     2701 cccgaggtag gtcatccccc cgaacacgtc tccgttggca atcctgaaat gctgcagcac
     2761 ctccacaccc acaggggagt cgatcacctc cgtcccccag ggcgcccgcg tgtcgattcc
     2821 ccaggtcggc gggctgccaa acgggtccag aggggtcggc aggtagctgg ccacgaccac
     2881 ctggatccct cctctgggat ggacatccag gttgtacgaa cctgcggcga aggctccctg
     2941 gacatcgccc cgctcaaaca gggcggccca attcgcccct ggaccctggg cacaggtcca
     3001 cggcggcctg aatgcggccc ccggccccag gtcatcgcag accggctcga tgaggcccgc
     3061 gatcgcgggg ttcgggaggg gctggtagag ggcgtccagc accccccccg gcccagcaac
     3121 cggcgtggag ggcaggatgg gggccggcgt cacatgcacg ggccatacga gcacatccgc
     3181 ggccgtgagg acgacgatcg ccgtgtcccc gggtcgcaca ccccggatgc cgatgatccc
     3241 cggctccact tcgcgcacat ccacgatcga gggatcaacc gcttcaacgt gcacggcgcc
     3301 gctggtggcc atccgaacct cggcccgggc tgccacctcg atggggcgcc cgagcttctg
     3361 tggcacgctc tgcggtagag cggctggcgg tgcaggcggt ggcgtcgccg ccggcggttc
     3421 cgcgccgggc gatcccggcc caccgcgtgc gggctcaccg cccaccggct gacggatctc
     3481 gggcgggggt gagggtgtga aatccatcgc gaaccccggt gcgcccacgc cctggaagat
     3541 agccccaccg tcgggtccgc tgccggtcga gatgagcgca tccggctgcg gtccggcggg
     3601 cgctgccccc gccccctcaa ggggcatcag gaccgatcca gcctccgaga cagaaacgat
     3661 ccggccgttc tgttccttcg tccacacgac ctcggcggcg cgtgggggtg ccggggccca
     3721 cccgaacgac tccggcaacg cggcaggagc caggacggct gcggcccagc agaggactag
     3781 ggtacctgcg aggaggcgat aaatggtgtg ctggcgccgt cgacggcaag cgccactctc
     3841 gcgaccgtcc ccgggccgag tggggcgggc catcgcagtg tcaggacggt cgttccacca
     3901 gggagcagcg cgaactcggg gacgtcgagc ctcccgagaa atgtcccgtc ggacctgagc
     3961 aggaacagcg ccccgcgccc cctgcagagc gccgcgttgg ggttctggat ctgcaattgc
     4021 acaagtccct gggcggtcac ggtgagctgc gtctgcggcg tgaggtctgc gccgggggcg
     4081 gccacgacca actgggacac gatccgggcg aacgccgcgg ggttggggcc gggggtgacg
     4141 gcgctgacca cgatggcgcc gaagcgtgtg tggccgtcgc cacggacgcg aatggcgagc
     4201 ttgcgcgttt cccggggggc gagcacgatc tcctggctcg gcagcgtgac gcttcccgtg
     4261 agcgtgtgcg gggttgtggc ggccgggcga accgtgcccg acggatcaag gtcggcgatc
     4321 tgcagctgca ccgtgagcgc agcctgggcg tcctcgttgc ggacgagaag atcggcgtcg
     4381 gcctcgacgc cggggcccac ggtgacctgc tggaggaccg gctgaccggc caagcccccc
     4441 gaagagggag cggccgcggg gcccgcggcg gccggcgcga tcaacagcag cagggcgacc
     4501 acgacaagcc ccatgggcgc cctgtgcctc cgaatcgacg aactccgaaa aggtcccgct
     4561 actgcgccga gtaggtgatg gtgatggtcg tgctcccggc gatcgcgctc ccaccggtcg
     4621 gcgcgaccaa tatatagtac ttcttcgt
//
LOCUS       DASQGH010000209         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20785, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000209 DASQGH010000000
VERSION     DASQGH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20785"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VEW91_03995"
     CDS             <1..900
                     /locus_tag="VEW91_03995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HYM90780.1"
                     /translation="AAAWARETRSELVSRAHVRRAVEEKAYRSNLLEERIRELIAKGV
                     ILVDTEGTVTGQINGLSVLQLGDYTFGQPSRITARAYVGGRGVVNIERESQMSGRIHS
                     KGVFVLSSFLANRFAQQHPLSVSASLTFEQLYSDVDGDSASSTELFALLSELAGLPID
                     QGIAVTGSVNQMGEIQPIGGVNEKIEGFYYTCKVKGVPGRQGVIIPHQNARDLMLREE
                     VVDAVKEERFHVWAVRTVDEGIEILTGTPAGGRDAEGVFPEDSVNGRVARHLAELARR
                     YREFAPGAGPGLGADPSKDTPPA"
     gene            1059..>2181
                     /locus_tag="VEW91_04000"
     CDS             1059..>2181
                     /locus_tag="VEW91_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYM90781.1"
                     /translation="MSIQSHGFTPAPEQIGALARQQAVVATLSQRALSGADDAALMSE
                     AVAAVAQALRVPFCNILERIPGTQDLRACAGAGWKGARERATVPGDPNSQAGYTLASG
                     KPVIVEDYRKEQRFKTAPLAEGTWVASGVSVPIPGREQPLGVLTVGSPTRRVFVAEDV
                     NFLQSVANVLAAALERHRAETTLRETNQQLRAVVHASPLAVISLDSSGCVRSWNATAE
                     RIFGWSADEVIGQPDPVLTQSNREEIYKLVDRALHGETFTEVATQVVKKDGLPVDVTV
                     SVAPIYDGEGATSGVIAVIADMTERRRTEAAQAQLTEIIETTTDCVIITNVPGRGFFI
                     NRAGREMLGIGQDEDASGVDLPDLYPGETKAFIVNEAMPA"
BASE COUNT          375 a          747 c          768 g          291 t
ORIGIN      
        1 gccgcggcct gggcccgcga gacgcgcagc gagctggtct cccgcgcgca cgtgcgccgg
       61 gcggtcgagg agaaagcgta ccgcagcaac ctgctcgaag agcggatccg ggagttgatc
      121 gcgaagggcg tgatcctcgt cgacaccgag ggcaccgtca cggggcagat caacggccta
      181 tcggttctgc agctggggga ctacaccttc gggcagccga gccggatcac cgcgcgcgcg
      241 tacgtgggcg gccgcggggt ggtgaacatc gagcgcgaga gccagatgtc ggggcggatc
      301 cacagcaagg gcgtgttcgt gctctcctcg ttcctggcca accggttcgc ccagcagcat
      361 ccgctcagcg tctcggcgtc gctgaccttc gaacagctct actcggacgt ggacggcgac
      421 agcgccagtt cgaccgagct gttcgcgctt ctgagcgagc tcgcggggct ccccatcgac
      481 cagggaatcg cggtcaccgg ctcggtcaac cagatggggg agatccagcc catcgggggc
      541 gtcaacgaga agatcgaggg cttctactac acgtgcaagg tcaagggggt gccggggcgg
      601 cagggggtta tcatcccgca ccagaacgcc cgcgacctga tgctgcgcga ggaggtcgtt
      661 gacgcagtca aggaagagcg cttccacgtc tgggcggtcc ggaccgtgga cgaagggatc
      721 gagatcctca ccggcacccc ggccggcggg agggacgccg aaggggtctt cccggaggac
      781 tcggtcaacg gccgcgtcgc ccgccatctc gccgaactgg cccgccggta ccgggagttc
      841 gcccccggcg cgggccccgg cctaggcgcc gatccctcca aagacacccc cccggcctag
      901 gggtccccag caaggcacac gcggcccccg gcgcggccgg cctggggcgc ggtcgcggcc
      961 aggcgtccag aaccgaacct ccgtgggcaa taaattgaag atagccctcc ggggggaggg
     1021 gaaggagccg gttccgagac catcaggaac ctccaatcgt gagcatccag tcccatgggt
     1081 tcacgccggc ccccgagcag atcggggccc tcgcgcgcca gcaggccgtc gtcgccaccc
     1141 tcagccagcg cgcgctctcc ggtgccgatg atgccgcgct gatgagcgaa gccgtggccg
     1201 ccgtcgccca ggccctgcgg gtaccgttct gcaacatcct ggagcgcatc ccggggaccc
     1261 aggatctccg ggcctgcgcc ggggccggct ggaagggcgc gcgcgagcgg gccacggtcc
     1321 cgggcgatcc caactcgcag gcggggtaca ccctggcctc cggcaagcca gtgatcgtcg
     1381 aggactaccg caaggaacag cggttcaaga cggcccccct cgccgagggg acctgggtgg
     1441 ccagcggggt gagcgtgccg atcccgggcc gggagcagcc ccttggggtg ctcacggtcg
     1501 gttcccccac ccggcgggtg ttcgttgcag aggacgtcaa ctttctgcag tccgttgcca
     1561 acgtgctggc cgcggcgctc gaacgccaca gggcagagac gacgctcagg gagacgaacc
     1621 agcaactgcg ggcggtcgtc catgcctcgc cgctcgcggt gatctcgctc gacagcagcg
     1681 ggtgcgtccg cagctggaac gccacggccg agcggatctt cggctggtcc gccgacgagg
     1741 tcatcgggca gcccgacccc gtcctgaccc agagcaaccg ggaggagatt tacaaactgg
     1801 tcgaccgggc cctccacggc gagacgttca ccgaggtcgc cacgcaggtg gtgaagaagg
     1861 acggcctgcc ggtcgacgtg acagtctcgg tcgcaccgat ctatgacggc gaaggcgcga
     1921 ccagcggcgt catcgccgtg atcgccgata tgaccgaacg ccggcggacc gaagccgcgc
     1981 aggcccagct gacggagatc atcgaaacca ccaccgactg cgtcatcatc accaacgtcc
     2041 ctgggcgggg attcttcatc aaccgggccg gccgcgagat gctggggatc ggccaggacg
     2101 aggatgcctc cggggtagac ctgcccgacc tgtacccggg ggaaaccaag gcgttcattg
     2161 tcaacgaggc gatgccggcc g
//
LOCUS       DASQGH010000210         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_15086, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000210 DASQGH010000000
VERSION     DASQGH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15086"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEW91_04005"
     CDS             complement(<1..195)
                     /locus_tag="VEW91_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYM90782.1"
                     /translation="MTELPGPEPTLFFDLYGTLVDIQTDEDDPWVYAMLARYLAYFRV
                     PISPEALQQEYRTRVRTHLER"
     gene            complement(192..1190)
                     /locus_tag="VEW91_04010"
     CDS             complement(192..1190)
                     /locus_tag="VEW91_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYM90783.1"
                     /translation="MSAVYLGMLDQGDPLYQVLALRVYPEIKRPAFSVERLSTHGLVY
                     RYREAETRRAVVGKFFNPQDRDERKVRRIKSEYRNLIRLRYLGFNAHPHYVVNPLCSE
                     ERIGLAVAEEFIRGKDLDHYLSHAANDGTGPALKEVLGGLAGFLYRLHSRSADRAAVR
                     LDEVAAYFAGVVGKLQQQGVITPYHVLEYLRLRDRWLQRPRMLAEAVTVHGDATPTNF
                     LFPTRDDVVAIDLERMKEADRAFDVGMVCGEIKHAFLWRTGRLDASEPFIRHFLERYV
                     RHTPSPDGAFWEITRRVPFYMALTELRIARNTWLDWAYRRRLVWEAHECLTWGLEL"
     gene            complement(1281..2528)
                     /locus_tag="VEW91_04015"
     CDS             complement(1281..2528)
                     /locus_tag="VEW91_04015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYM90784.1"
                     /translation="MPPQDPWDMAMRQFEAAADHLTLKRGIRDYLAFPNRELVVHFPV
                     EMDDGSVRLFTGYRVHHSTVLGPTKGGIRYSPEVRLNEVRALAMWMTWKSALMHLPYG
                     GAKGGVACDPRHLSHDELQRLTRRYATEVSLLMGPQSDIPAPDVGTTAQIMAWIMDTY
                     SMQRGYSVPSVVTGKPIVIGGSMGRSDATGRGVMLITREAARVRGIPLAGSRVVIQGF
                     GNVGEAAARLLHAQGCRIVALSDVDGGIYNPAGLDPQAVLGHVRTAGGLARYPSAEPV
                     SNAELLELPCDVLVPAAIEGQITADNAARIRAAVIVEGANGPTTPEADAILASRDVLV
                     VPDILANAGGVSVSYFEWVQDLQSYFWSEEEINAHLERIMVSTFERVAAVARDRRVTL
                     RMAALIVAVRRVADALMIRGIYP"
     gene            2662..>3705
                     /locus_tag="VEW91_04020"
     CDS             2662..>3705
                     /locus_tag="VEW91_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM90785.1"
                     /translation="MKMETLDTLVREDQAHLVHPLHHPQDHQHPIVFVEGRGAILKDL
                     EGHEYIDGLSCLWNVNVGHGRAELAQAAAEQMARLAFASSYVGTTNIPAIRLAERLVA
                     LASPNTSGVYFTTGGAESNESAFKIARYYWKVRGRPTKVKIFSRVHAYHGLTLGAMSA
                     TGMAAFHKMFGPLAPEFAQVAAPYAYRWPTAGDVGLEAADALEQAILRDGPDTVAAFI
                     AEPVMGAGGVIPPPPSYFPRIREICDRHDVLLIADEVITGFGRTGRWFAMGHWNVQPD
                     LVTFAKGVTSAYLPLGGVMFNKRVHDAVLEAPPDKKFMHAATYSGHPVCCAVALRNLD
                     ILEQEGLVERAGPL"
BASE COUNT          590 a         1261 c         1256 g          598 t
ORIGIN      
        1 ccgttccaga tgggtccgca cccgggtgcg gtactcctgt tgcagcgcct cggggctgat
       61 cggcacccgg aaataggcga gataccgcgc gagcatggcg tagacccacg gatcgtcctc
      121 atcggtctgg atatcgacca acgtgccgta gaggtcgaag aagagtgtgg gctcgggccc
      181 tggaagttcg gtcacagctc gaggccccag gtgagacatt cgtgcgcttc ccacacgagg
      241 cgccgccgat acgcccagtc cagccaggta ttgcgggcga tccgcagttc ggtcagcgcc
      301 atgtagaatg ggacccggcg cgtgatctcc cagaaggcgc catcgggcga cggggtatgg
      361 cgcacatacc gttcaaggaa gtgtctgatg aatggttcgg acgcatccag gcgccccgtc
      421 ctccagagga acgcgtgctt gatttcgccg cacaccatcc ccacgtcaaa ggcccggtcc
      481 gcctccttca tgcgttcaag atcgatggcc acgacgtcgt cccgggtggg gaacaagaaa
      541 ttcgtcggcg tcgcatcgcc gtggacggtg accgcttccg ccagcatgcg gggccgttgc
      601 agccaccggt cccgcagccg caggtactcc agcacgtgat agggggtgat gaccccctgc
      661 tgctgcagct tgccgacgac cccggcgaag taggcggcga cctcgtccag gcgcacggcg
      721 gcgcggtcgg cggatcggct gtggaggcgg tagaggaagc cggcgaggcc ccccaggacc
      781 tccttgagcg cggggccggt cccgtcattg gccgcgtgcg acaggtaatg atcgaggtcc
      841 ttgcccctga tgaactcctc cgcgacggcg aggccgatgc gctcctcgct gcacagcggg
      901 ttgaccacat agtgcggatg ggcattgaat cccaggtacc gcagccggat caggttccgg
      961 tattcgctct tgatccggcg caccttgcgt tcgtcgcgat cctgggggtt gaagaacttc
     1021 ccgacgaccg cgcgtcgtgt ctcggcttcc cgataccggt agaccaggcc gtgggtggac
     1081 aggcgctcca cagagaaggc gggccgcttg atctccgggt agaccctgag cgcaagtacc
     1141 tggtacaggg gatcaccctg gtcgagcatg cccagataca cggcactcat ggggaggtcc
     1201 gtccgcggag atccactcgg agattcactc ggggttatac gctcctgggg atccgggtat
     1261 tccgctgtcc cgccgcgcgt ctacggatag atgccccgga tcatcagcgc atcggcgacg
     1321 cgccgaaccg cgacgatcaa cgcggccatc cgcagcgtca cccgccggtc ccgggccacc
     1381 gccgcgaccc gctcaaaggt ggagaccatg atgcgctcca gatgcgcgtt gatctcctcc
     1441 tcgctccaga agtacgattg gaggtcctgg acccactcga agtaagagac gctcaccccg
     1501 ccggcgtttg ccaggatgtc gggaacgacc aggacgtccc ggctggcgag gatcgcatcc
     1561 gcctccggcg tggtcggccc gttcgcgcct tctacaatga cggcggcccg gatgcgcgcc
     1621 gcgttgtcgg cggtgatctg cccctcgatc gcggccggca ccagcacgtc gcacgggagc
     1681 tcgaggagtt ccgcgttgga gaccggttcg gcgctcgggt agcgggccag gcccccggcg
     1741 gtgcgcacgt gccccaagac ggcctgcgga tccagccccg cggggttata gatgcctccg
     1801 tcgacgtcgc tcagggctac gatcctgcac ccctgtgcgt gcaggagacg ggctgccgcc
     1861 tcccccacgt tgccgaaccc ctggatgacc acccggctgc cggccagcgg gatcccccgc
     1921 acccgcgcgg cctcccgggt aatgagcatg accccccggc cggtggcatc gcttcggccc
     1981 atggagcctc cgatcacaat cggcttcccc gtgaccacgg acgggaccga atagccccgc
     2041 tgcatgctgt acgtgtccat gatccaggcc atgatctgcg cggtggtccc gacgtcgggc
     2101 gccgggatgt cactctgggg acccatgagg aggctcacct cggtcgcgta ccgccgggtc
     2161 agccgctgga gttcgtcgtg cgagaggtgc cgcgggtcgc acgcgacgcc gcccttggcg
     2221 cccccgtacg gcaggtgcat gagggccgac ttccaggtca tccacatcgc cagcgcccgg
     2281 acctcgttga gccgcacctc ggggctgtag cggatcccgc ccttggtggg gcctagtacg
     2341 gtgctgtggt gcacccggta cccggtaaag agccggacgg agccatcatc catctcgacg
     2401 gggaagtgca ccaccagttc gcggttgggg aaggcgaggt agtcgcggat gccgcgcttg
     2461 agcgtgagat ggtcggcggc ggcctcgaac tggcgcatgg ccatgtccca cgggtcttgc
     2521 ggcggcatag gtggtgcctg tttcgcatgc ccggggggag ttcctctgcg cgtgcagcct
     2581 gcggcgacca ggagatggcg gggccgggca gaagaagact aggagcaccc cgtctcgagg
     2641 cggggaggtc ggagggatgg catgaaaatg gagacgctgg acacgcttgt ccgcgaggac
     2701 caggcccacc tggtgcaccc gctccaccac ccgcaggacc accagcaccc catcgtgttc
     2761 gtggagggcc ggggggcgat cctcaaggat cttgagggtc acgaatacat cgacgggctg
     2821 tcgtgtctct ggaacgtgaa cgtggggcac ggccgcgcgg agctggccca ggccgctgcc
     2881 gagcagatgg cccggcttgc gttcgcctcc agctacgtgg gcacgaccaa catcccggcg
     2941 attcgcctcg ccgagcggct cgtcgccctg gcgtccccca acacgtcggg cgtctacttc
     3001 acgaccgggg gggccgagtc caacgagtcc gcgttcaaga tcgcccgcta ctactggaag
     3061 gtccggggcc gcccgaccaa ggtcaagatc ttctcccggg tccacgccta ccacgggctc
     3121 accctgggcg cgatgagcgc caccgggatg gccgcgttcc acaagatgtt cggcccgctg
     3181 gcccccgagt tcgcccaggt ggccgctcca tacgcgtacc gctggccgac cgcgggcgat
     3241 gtcggattgg aggcggcgga cgcgctcgag caggccatcc tccgggacgg gcccgacacc
     3301 gtcgcggcgt tcatcgccga gccggtgatg ggggccggcg gggtgatccc ccctcccccc
     3361 tcctacttcc cccgcatccg cgagatctgc gaccggcacg atgtcctgtt gatcgccgac
     3421 gaggtcatca ccgggtttgg acggaccggc cggtggtttg cgatggggca ctggaacgtc
     3481 cagccggatc tcgtcacgtt tgccaagggg gtgacgagcg cctaccttcc cctgggcggg
     3541 gtgatgttca acaagcgcgt gcacgacgcg gtcctggagg cgcccccgga caagaagttc
     3601 atgcacgcgg cgacctactc cgggcacccc gtctgctgcg cggtcgccct ccgcaacctc
     3661 gacatcctcg agcaggaagg cctggtcgag cgggccggcc cgctc
//
LOCUS       DASQGH010000211         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40557, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000211 DASQGH010000000
VERSION     DASQGH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40557"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VEW91_04025"
     CDS             <1..1074
                     /locus_tag="VEW91_04025"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90786.1"
                     /translation="DAEGMLDQLNAFCQGRIAKADVLAVLGLIEEEVAQKVADAVIAQ
                     DAAACLEIANRIIAEGRDVRQILHSLTEHFRDLLVLSMVRDPRDIVETSEGRLEILRG
                     QSACLSPGALLQKIRILTTAEADARFATHPRVVLEMALLKLCRPEMDPSFEGLAARVE
                     ALEQQAARAQAQVPPALEAPDTPPSGEEAPVRTPSPASRPPRRGARGRESRADAVAGG
                     ADAAAPHGAAPRAGGLEDAPGAGIDLVRARWGRLMEEVKQRSRPIHAFLLESAPRAVE
                     GTSLVLAVRHKFHLESLQERKNRQLVEELLATVMGVPLRLGLVLGEETASQAAAVPGG
                     SDTLVEEAVRRFGNPVQEIRHPE"
     gene            1132..1464
                     /locus_tag="VEW91_04030"
     CDS             1132..1464
                     /locus_tag="VEW91_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYM90787.1"
                     /translation="MFNMQKMLKQVQKIQEDMARVQEELARERIEASAGGGVVRAVVS
                     GQGDLMEIAIDPSVVDPSDVTMLQDLVLAACSEAIRKARDHAAERMKGITGGLQLPGL
                     PGLPGLGA"
     gene            1472..2071
                     /gene="recR"
                     /locus_tag="VEW91_04035"
     CDS             1472..2071
                     /gene="recR"
                     /locus_tag="VEW91_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYM90788.1"
                     /translation="MFSYAPPLARLIEELSKLPTIGPKTAQRLAFHMLSMAPQDAEAL
                     AEAILEARRRIRHCSVCANITEADPCELCTNTQRDHAVICVVESPRDMAAMERTREFK
                     GVYHVLQGAISPLDGIGPDDLRIAELLRRLTDGAVREVIIATNPRVEGEATALYLSKV
                     IKPLGVRVTRIAHGLPVGGDLEYADEVTLARALEGRREL"
     gene            2096..3823
                     /locus_tag="VEW91_04040"
     CDS             2096..3823
                     /locus_tag="VEW91_04040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HYM90789.1"
                     /translation="MPAIDPRKEFPRRFVPPDASAGRWEEIERLGQALLRTPPATPEG
                     LERWLEDASELEAVVAEEGARRYIAMTSQTDDPERERVHLHFVREIEPRWKQLAHRLD
                     AAYLANPHRRALPERYRVLDRKVENRVALYREENLPLQVAEEELGQQYQKLRGAMTVV
                     FRGREHTLEQLSRYLRDPDRAVRQEVWVLSAARMRRDREALEDLFDRLRALRLQIARN
                     AGFASYRDYAFRARERFDYGPEDCRRFQDAVEACFVPLSDRVAKERQRLLGVETLRPW
                     DLDVDPLRRPPLQEFDRVEDLLDRGEAVFRSVHPELGDHFRFMRDRGVFDLERRKGKA
                     PGGYQQTLDERRWPFIFANVPAAEDVRVVLHESGHAFHTMAVRDEPLMAYRHAPLEFA
                     EVASMGMELLAAPHLGRIYPGPDQARRAYRELLEGILVPRGMPWIATVDAFQHWVYTH
                     PAHTPDERRRAWLEIYRRFHRAVDWTGYEDVLGDLWHRQLHIFLLPFYYIEYGVAQLG
                     ALQIWLHATRSNVDEAVARYRSGLALGGSRPLPQLFEAAGAHLAFGEETMRPLADALG
                     EELARTPYA"
     gene            3922..>4266
                     /locus_tag="VEW91_04045"
     CDS             3922..>4266
                     /locus_tag="VEW91_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HYM90790.1"
                     /translation="MEFISPTHGRLTFERMFRQLVAYMREEPAERYNLIIGTDSLLGD
                     DTCFVTAIIVHRVGHGGRYFYHKFRNRKIESLRHRILFETSLSLETASLVSAQLVKNG
                     YRELPVEMHLDVG"
BASE COUNT          661 a         1459 c         1527 g          619 t
ORIGIN      
        1 gacgccgagg ggatgctcga ccagctcaac gcgttctgcc aggggcggat cgccaaggcg
       61 gacgtgctgg cggtcctggg cttgatcgaa gaggaagtgg cccaaaaggt cgccgacgcg
      121 gtgatcgcgc aggatgcggc ggcgtgcctc gagatcgcca atcgcatcat cgcggagggc
      181 cgggacgtcc ggcagattct gcacagcctc accgagcatt tccgcgatct cctcgtgctc
      241 tcgatggtac gggatccacg agacatcgtg gagaccagcg agggacgtct ggagatcctg
      301 cgcggccaga gcgcgtgcct ctcccccggg gcgctgctgc agaagatccg gatcctgacc
      361 acggccgagg cggacgcccg gttcgccacg cacccgcggg tggtcctgga gatggccctc
      421 ttgaagttgt gccggcccga gatggatccc tcgttcgagg gattggccgc gcgggtcgag
      481 gccctcgagc agcaggccgc tcgggcccag gcccaggtgc cccccgcgct ggaagcgccc
      541 gacacccctc cgtcggggga ggaggccccc gtgaggaccc cgtccccggc gagccgtccc
      601 ccgcggcgcg gcgcccgcgg ccgcgagtcc cgcgctgatg cggtggccgg tggggctgac
      661 gctgccgccc cccacggcgc ggccccgcgc gcgggaggcc tcgaggacgc ccccggcgcc
      721 gggatcgatc tcgtccgcgc ccgctgggga cgcctcatgg aagaggtgaa gcagcgcagc
      781 cggcccatcc acgcctttct cctggagagc gccccccggg cggtggaggg aacgagcctc
      841 gtgctcgcgg tccggcacaa gttccatctg gagagcctgc aggagcgcaa gaaccggcag
      901 ctcgtggagg aactcctcgc caccgtgatg ggggtgccgc tccggctcgg gctggtgctc
      961 ggcgaagaga cggcttcgca ggctgcggcc gtccccgggg gttccgatac gctcgtcgag
     1021 gaggccgtgc gccggttcgg caaccccgtc caggagatcc ggcatcccga gtgaccaatc
     1081 cacagtgacg ggcgtgttcg atcggaacgc tcgcgcggag cggagcgacg catgttcaac
     1141 atgcagaaga tgctcaagca ggtgcaaaag attcaagaag acatggcccg ggtgcaggaa
     1201 gagctggcgc gcgagcggat cgaggccagc gccggcggcg gtgtggtccg ggccgtcgtg
     1261 agcggccagg gcgatctcat ggagatcgcc atcgacccca gcgtggtgga cccgtccgac
     1321 gtcacgatgc tccaggatct cgtcctggcg gcctgcagcg aggcgatccg gaaggcgcgt
     1381 gaccacgccg ccgagcggat gaaggggatc acggggggcc tgcagctgcc ggggctcccg
     1441 ggccttcccg gattgggcgc gtgaggccgg gatgttttcc tacgccccgc cgctggcccg
     1501 cctgatcgaa gaattgagca agctgcccac gatcgggccc aagaccgcgc agcgcctggc
     1561 gttccacatg ctctcgatgg ctccccagga cgccgaggcc ttggccgagg cgatcctcga
     1621 ggccaggcgc cggatccggc actgttcggt gtgcgcgaac atcaccgaag cggatccgtg
     1681 cgagctgtgc acgaacacgc agcgcgacca cgccgtgatc tgcgtcgttg agagcccccg
     1741 agacatggcc gcgatggagc ggacccggga gtttaagggc gtctaccacg tgctgcaggg
     1801 cgcgatctct ccgctcgatg ggatcggacc cgacgacctt cggatcgccg agctcctccg
     1861 ccgcctcacc gatggggcgg tgcgggaggt catcattgcg accaacccgc gggtcgaagg
     1921 cgaagcgacg gcgctgtacc tctccaaagt gatcaaaccg ctgggggtca gggtgacccg
     1981 gatcgcgcac gggctgcccg tgggcgggga cctcgagtac gccgacgagg tcacgctcgc
     2041 gcgggcgctc gaggggcgcc gggagctgta ggcctcaccg gtcggggaca ccctcatgcc
     2101 ggcgatcgac ccgcggaagg agttcccgcg ccggttcgtc cccccggacg cgagcgccgg
     2161 ccggtgggaa gagatcgagc ggctcggcca ggccctcctc cgcacgcccc cggccacgcc
     2221 cgaggggctc gagcggtggc tcgaggacgc cagcgagctc gaagcggtgg tggccgagga
     2281 gggcgcccgc cgctatatcg cgatgacgtc ccagaccgac gacccggagc gggagcgcgt
     2341 ccacctccac ttcgtgcgag agatcgagcc gcggtggaag cagctcgcgc accggctgga
     2401 cgcggcctac ctggcgaacc cgcaccggcg tgcgctcccc gaacgctacc gcgtgctcga
     2461 ccggaaggtc gagaaccggg tcgccctcta ccgggaggag aaccttcccc tccaggtcgc
     2521 ggaagaagag ttggggcagc agtaccagaa gctacggggc gcgatgacgg tcgtcttccg
     2581 cggccgggag cacaccctgg agcagctgag ccggtatctc agggaccccg accgggcggt
     2641 ccggcaggaa gtctgggtgc tcagcgcggc gcgcatgcgc cgggaccggg aggcgctcga
     2701 ggatctcttc gaccggcttc gggcactccg gctccagatc gcgcgcaacg cgggcttcgc
     2761 ctcgtaccgc gactatgcgt ttcgggcgcg cgagcggttc gactacgggc cggaggactg
     2821 ccggcgcttc caggacgccg tcgaggcgtg cttcgttccc ttgagcgacc gggtggcgaa
     2881 ggagcgccag cgcctcctgg gcgtggagac gctgcggccg tgggatctcg acgtcgatcc
     2941 gctccgccgt ccgccgcttc aggagttcga tcgggtcgaa gacctcctcg accgcggcga
     3001 ggcggtgttt cggagcgtcc accccgagtt gggcgaccac ttccggttca tgcgcgaccg
     3061 gggggtgttc gacctggaac ggcggaaggg gaaggccccc ggcggttacc agcagaccct
     3121 cgacgagcgc cggtggccgt tcatctttgc aaacgtgccc gccgccgagg acgtccgcgt
     3181 cgtgctccac gagagcgggc atgccttcca caccatggcg gtgcgcgacg agccgctcat
     3241 ggcctaccgg cacgcgccgt tggagttcgc cgaggtggca tcgatgggca tggagctgct
     3301 ggccgcgccc catctgggcc gcatctaccc cggccccgac caggcccgcc gcgcgtaccg
     3361 tgagttgctc gaggggatcc tggtaccgcg cggcatgccg tggatcgcca cggtcgacgc
     3421 gtttcagcac tgggtgtaca cgcatcccgc gcacacgccg gacgagcggc gacgggcgtg
     3481 gctcgagatc taccgccggt tccaccgtgc ggtcgattgg acggggtacg aggacgtcct
     3541 gggcgacctc tggcaccggc agctccacat cttcctcctg cccttctact acatcgagta
     3601 cggcgtggcc cagctgggcg cgcttcagat ctggctccat gcgacgcgct cgaacgtcga
     3661 cgaggccgtc gcccgctacc ggagcgggct ggccctgggc gggagccggc cgctcccgca
     3721 gctcttcgaa gccgcggggg cacatctcgc gttcggcgag gagacgatgc ggcctctcgc
     3781 ggacgccctc ggagaggaac tggcccggac cccctacgcc tgacggccgg cggggtccgc
     3841 cggcgggcca tttgcaggcg tgtcgaggca tcgcgccttg ccccctcacc ccgcgtttcg
     3901 gtatacttgg gcaaggtgcc tatggaattt atcagcccaa cgcacggcag actcacgttc
     3961 gagcggatgt tccggcagct cgtggcctac atgcgggagg aacccgcgga gcggtacaac
     4021 ctgatcatcg gcaccgactc gctgctgggc gacgatacct gttttgtcac cgcgatcatc
     4081 gtccaccggg tcggtcacgg cggacggtac ttctaccaca agttcaggaa ccgaaagatc
     4141 gagagccttc gccaccgcat tctcttcgag acctcgctca gcctggagac ggccagcctg
     4201 gtgagcgcgc agctggtcaa gaacgggtac agagagctgc cggtggagat gcacctcgat
     4261 gtcggc
//
LOCUS       DASQGH010000212         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12299, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000212 DASQGH010000000
VERSION     DASQGH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12299"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..>1519
                     /locus_tag="VEW91_04050"
     CDS             467..>1519
                     /locus_tag="VEW91_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYM90791.1"
                     /translation="MAEIPSAYEPARVEEQRYAGWLAHGEFHTVPDPARRPYVIMMPL
                     PNVTGELHMGHALNNSLQDCLIRWSRMRGYNAMWQPGLDHASIAVHVVMERRLAQRGL
                     TRFDLGRQRFLEETWKWKEQIGGTILGQLRRLGCACDWDRTVFTMDPGYVDAVQESFI
                     RLFRKGLIYKGKRMINWCPKDQTSIADLEVEHVETPSILYYVRYPGEDGGEGVVVATQ
                     RPETILADVAVAVHPDDARYRRLVGTRVRVPAVGRVVPVIADRRVDRGFGTGAVKITP
                     GHDPLDNEIGAEHGLPVLVCLDPQARVNDLGGERYRGMDRDGCRKALTEDLRAAGALE
                     REEAYTTNIGRCERCKT"
BASE COUNT          255 a          478 c          572 g          214 t
ORIGIN      
        1 acaatagcgt ccggccccac caggccctgg gctatctcac cccgaacgag ttcatcgctc
       61 ggtggcaggc tacgcatggc cacgaaagga ggcgtgtgta gtcggatcta ctggaccagt
      121 acaggcgctt gctcttgcgt cgcgggaact gctataatgc cggcaatcgt cgtacgctcg
      181 gaaggacgcg aggacgggga ggagtagacc cgccgcccga aagcccccca tccgggggac
      241 aagcgagggg aggagttggt gagagctccc cgggccgcgc aggtcgaagg tcgcccctga
      301 gcggcgggcc gacagcgcgc gtggcgcccg aggcccgatc gggtgcgccc gtgacagcgt
      361 tcgagccggc gggccgacga ggccgccggg acaaaggtgg ttccgcggag cgccgctctg
      421 ccctttgagg cggagcggtg cggtgttttt aaggaggggc atggtcgtgg ccgagatccc
      481 cagcgcctat gaaccggcgc gggttgagga gcagcggtac gcgggctggc tggcccacgg
      541 ggagttccat accgtcccgg acccggcgcg gcggccctac gttatcatga tgccgctccc
      601 caacgtcacc ggggaactgc atatgggcca cgcgctcaac aacagcctgc aggactgtct
      661 catccggtgg agccggatgc gcgggtacaa tgcgatgtgg cagccggggc tcgaccacgc
      721 cagcatcgcc gtgcacgtgg tcatggaacg gcggctcgcg cagcggggcc tgacccggtt
      781 cgacctcggc cggcagcggt tcctcgagga aacctggaaa tggaaggagc agatcggcgg
      841 gacgatcctc ggacaactcc gccgcctggg atgcgcgtgc gattgggatc ggacggtctt
      901 cacgatggac cccgggtacg tggacgcggt ccaggagtcg ttcatccggc tctttcgcaa
      961 ggggttgatc tacaagggga agcggatgat caactggtgc cccaaggacc agacgagcat
     1021 cgcggacctc gaggtggagc acgtcgagac gccgagcatc ctctattatg tccgctaccc
     1081 cggcgaggac ggcggggagg gcgtggtggt ggccacccag cgtcccgaga cgatcctggc
     1141 cgacgtcgcg gtggccgtcc accccgacga cgcgcggtac cgccggctgg tcgggacccg
     1201 ggtgcgcgtc ccggccgtgg ggcgcgtggt gccggtgatc gcggaccgcc gggtggaccg
     1261 cgggtttggg accggggcgg tgaagatcac gcccgggcac gacccgctcg acaatgagat
     1321 cggcgccgag cacgggctcc ccgtgctcgt gtgcttggat ccgcaggccc gggtcaacga
     1381 cctggggggc gagcgttacc ggggcatgga tcgggacggg tgccggaagg cgctcaccga
     1441 agacctccga gcggcgggcg ccctcgagcg cgaagaggcc tacacgacca acatcggccg
     1501 gtgcgagcgg tgcaagacg
//
LOCUS       DASQGH010000213         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3794, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000213 DASQGH010000000
VERSION     DASQGH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..515)
                     /locus_tag="VEW91_04055"
     CDS             complement(30..515)
                     /locus_tag="VEW91_04055"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HYM90792.1"
                     /translation="MFDDVTHRVIQVMLISIAPAAELRVAIPFGITKGLHPLVAFLAA
                     VVATWAVIVPMFLILDLFYEGFLSNVSLIRRIVHEVRHRGGTYVERWGVLGVGIYVSL
                     PLPGPGIYSGAILAWLFGLPRRRAIVSLALGAAASGVLVAAISTGVIALIRKFMQSSG
                     Y"
     gene            complement(505..1455)
                     /locus_tag="VEW91_04060"
     CDS             complement(505..1455)
                     /locus_tag="VEW91_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HYM90793.1"
                     /translation="MVHLVFPEDISPRGTLYGGRMMYWIATAGTLTASRCARGPVVLG
                     AMDDLDFLHPAYLGEIVTVSCQVEYIGRASLELGVEVQSEHPRTGLHRKTTSSHLAFI
                     ALDEREHPRPVGVVITPASDAEAGAHAAAAARKAARTARVRDAGAAGDLGLQHEGIRR
                     GVELSRLIAPEDALSGTLMFAGKLLAIIDEAAAISVIRYCHLPIVTASLDTVYFYHPL
                     RVGQVADLRAVPTHVGRSSMEIGVWVDGEEPRTGERRHTCTAFLTMVHLDAEGRPAPV
                     PPFTPETPEERRLWREAEARRAQRKVRVAQLRERVGHDVR"
     gene            1548..>2067
                     /locus_tag="VEW91_04065"
     CDS             1548..>2067
                     /locus_tag="VEW91_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90794.1"
                     /translation="MTLRYALSEYRRPAARRAFGRAWFWVCAAGALTSVVLFGSNHAF
                     FIAAVWLVCVFAPVRIAVEALHSRGPRMRRQLQQALAGREDRYGTSEHVTLMVETLFA
                     REVHLPRLAPPDLGDKVIEAAARLSDRALRTGAGPAAVFQAAATCATLLHRWAGAIAG
                     GEAGGAPPGGPGR"
BASE COUNT          321 a          742 c          716 g          288 t
ORIGIN      
        1 accccccttt tgccagcgcc ggcggtggct caatagccgg atgactgcat gaattttcgg
       61 atgagcgcga tcacccccgt gctgatcgcc gccacgagca ccccgctggc cgccgcgccg
      121 agcgcaagcg agacgatcgc ccggcggcgg ggcagcccga agagccaagc cagaatcgcc
      181 ccggagtaga tccccggccc gggcaggggc aggctgacgt agatccccac gcccaggacc
      241 ccccaccgct ccacataggt accgccccgg tgccggacct catggacaat gcggcggatc
      301 agggagacgt tggacagaaa cccctcgtag aagaggtcca ggatcaggaa catcgggacg
      361 atcaccgccc acgtggcgac gaccgcggcc aggaacgcca ccaaggggtg cagccctttg
      421 gtgatcccga agggaatcgc cacgcgcagt tccgccgccg gggcgatcga gatgagcatc
      481 acctggatga cgcggtgcgt cacgtcatcg aacatcgtga cccacccgct ctcgcaactg
      541 cgccacccga accttgcgct gggcgcggcg cgcttcggcc tcccgccaca gccggcgctc
      601 ctccggcgtc tccggggtga acgggggaac cggcgcgggg cggccctccg catccaggtg
      661 gaccatcgtg aggaatgcgg tgcacgtatg gcgccgctcc ccggtgcgcg gctcttcccc
      721 atccacccag accccgatct ccatcgagga acggccgacg tgggtcggga cggcccgaag
      781 atcggcgacc tgccccacgc gcagcgggtg gtagaaatag acggtgtcga gcgacgccgt
      841 gacgatcggc aggtgacagt agcggatcac cgagatcgcg gcggcctcgt cgatgatggc
      901 cagcagcttc ccggcgaaca tcagcgtgcc gctgagggca tcctccggcg cgatcaggcg
      961 gctcagctcc acaccgcggc ggatgccttc gtgctggagg cccagatcgc cggcggcgcc
     1021 ggcatcgcgg acccgcgcgg tccgcgccgc cttccgcgcg gcggccgccg cgtgcgcgcc
     1081 cgcctcagcg tcggacgccg gggtaatcac cacccccacc gggcgggggt gctcgcgctc
     1141 gtcgagcgcg atgaacgcca ggtgcgagga ggtcgtcttc cggtggaggc cggtccgcgg
     1201 gtgctccgac tgcacctcca cgccgagttc gagggacgcg cggccgatgt actcgacctg
     1261 gcaagacacg gtgacgatct caccgaggta cgcgggatgg agaaagtcga gatcgtccat
     1321 cgcccctagc acgaccgggc cccgggcaca gcgcgaggcg gtgagggtgc ccgccgtcgc
     1381 gatccagtac atcatccggc cgccgtatag ggtgccccgc gggctgatgt cctcgggaaa
     1441 cacgaggtgc accatttgaa accgcgtttc cgcgatgctt ggcagcccgc cctcctcgct
     1501 ttccctggct atggtaccat atacccgtcg atgtgcaagg atagtcgatg accctccgct
     1561 acgcgctctc cgagtaccgc cgccccgccg ccagacgcgc attcggccgg gcgtggttct
     1621 gggtctgcgc cgccggcgcg ctcacgagcg tggtgctgtt cgggagcaac cacgcgttct
     1681 tcatcgccgc ggtctggctg gtctgcgtgt ttgcgccggt gaggatcgcg gtggaagccc
     1741 tccactcgcg cggcccgagg atgcgccggc agctgcagca ggcgctggcc ggccgcgagg
     1801 accggtacgg gacgtctgaa cacgtcaccc tgatggtgga gacgctgttc gcgagggagg
     1861 tgcaccttcc ccggctcgcc ccgcccgatc tgggggacaa ggtgatcgag gctgccgccc
     1921 gtctcagcga tcgagcgctc cgcacaggtg ccgggccggc cgcggtcttc caggcggccg
     1981 ccacctgcgc gacccttctc caccgatggg ccggcgcgat cgccggcggc gaggccggcg
     2041 gggcgccccc gggggggccc gggagat
//
LOCUS       DASQGH010000214         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37814, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000214 DASQGH010000000
VERSION     DASQGH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37814"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1648
                     /locus_tag="VEW91_04070"
     CDS             176..1648
                     /locus_tag="VEW91_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYM90795.1"
                     /translation="MDPHRQTRLTSRLALVVLVLLAVAAPGRVSWAQTQGPPKHPNIV
                     FVLTDDLAWNLVQYMPHVLKMQKEGVTFSNFFVTDSLCCPSRSSIFTGRYPHNTGVFK
                     NVGKDGGYAVFERRGNPQATFATALSSAGYRTAMLGKYLNGYRPRTDRADPGWALWAV
                     AGDAYREFNYDLNEDGKVVHYSNKPADYLTDVASGLAVDFIKRAAGTPFAIEIATFAP
                     HGPYIPAPRDANKFPGLGAPRTAAFNATPDASTTKWLSRQPPLSNADGARIDKAFRMR
                     AQSVLAVDKMIGELQAAVAAIGEENNTYFVFSSDNGLHMGEYRLMPGKMTAYDTDIRV
                     PLIVTGPRVPAGRTVEQIAMNIDLCPTFSELAGAAAPANVDGRSLVPLLRGQNVADWR
                     TAALVEHLGPANDPADPDVPEPRSGNPTTYDALRTRTSLYVEYADGEREYHDLAADPD
                     ELRNTFASLSGPQKAALSATVKAIRNCHGANSCWPAEHAK"
     gene            1887..2432
                     /locus_tag="VEW91_04075"
     CDS             1887..2432
                     /locus_tag="VEW91_04075"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYM90796.1"
                     /translation="MRRVLAILGSALFLLIAAGIGAGVVPWWISRWDIQAPLFGFPPI
                     RVLGVLLIVAGIPVLLDSFARFALQGIGTPAPVFPTLHLVVKGFYRYVRNPIYLAVVA
                     VILGEGVILGNVNLLAYGVLAWLGFYLFVLGYEEPTLRKTFGAEYDTFCANVPRWVPR
                     LTPWSESAGRTANPAPPPPRG"
     gene            2506..2579
                     /locus_tag="VEW91_04080"
     tRNA            2506..2579
                     /locus_tag="VEW91_04080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2538..2540,aa:Ile,seq:cat)
BASE COUNT          481 a          855 c          830 g          494 t
ORIGIN      
        1 cttcttcatc ttctcccgct caatacggcg cgggcggtgc gggtcctctg gaggattttc
       61 gcgcggtgag gcgtatgatt ggcgcagcac gccgcccgcg tccaagcggt cctcgagctc
      121 gccgagcagc gctgacgacg cctctcgcga ggcaggccat ggcagggggt ggtgcgtgga
      181 tccacaccgg caaacccggt tgacgagccg cctggccctt gttgtcctgg tgctcctcgc
      241 ggtcgccgcc ccggggcggg tgtcttgggc acagacccaa ggccccccca agcacccgaa
      301 catcgtcttc gtcctcaccg acgatctcgc gtggaacctg gtgcagtaca tgccccacgt
      361 cctgaagatg caaaaggagg gcgtcacctt tagcaatttc ttcgtcaccg actcgctgtg
      421 ctgcccctcg cgctcgtcga tcttcacggg acgctacccg cacaacaccg gcgtcttcaa
      481 gaacgttggc aaagacggcg gctacgccgt atttgaaaga cgtgggaacc cgcaggcgac
      541 attcgccacg gcgctgtcgt cggccgggta tcgcaccgcc atgctcggaa agtacttgaa
      601 cggctataga cctcggacgg accgcgccga tcccggctgg gcgttgtggg cggtcgcggg
      661 agacgcgtat cgggagttta actatgacct caacgaagac ggcaaggtcg tccactacag
      721 caacaagccg gcggattact tgacggacgt cgcctcgggt ttggctgtgg acttcatcaa
      781 acgggcggcc ggaacgccgt ttgcgatcga gattgcgacc ttcgcgccgc acgggccgta
      841 catccccgcg ccgcgcgacg cgaacaaatt ccccggcttg ggcgcccccc gaacggcggc
      901 gttcaatgcc acgcccgacg ccagcaccac taaatggctg agcaggcagc cgccgctctc
      961 caacgccgat ggggcccgga tcgataaggc gttccgcatg cgcgcccaat cggtcctcgc
     1021 cgtcgacaag atgatcggcg agttgcaggc ggccgtggcc gcgatcggcg aagagaacaa
     1081 tacgtacttc gtcttcagct cggacaacgg gttgcacatg ggcgagtacc gtctgatgcc
     1141 gggaaagatg acggcgtacg acacggatat ccgcgttccg ctcatcgtca ccggaccgcg
     1201 tgtgcccgcg ggacgcacgg tcgaacagat cgccatgaac atcgacctct gcccgacatt
     1261 cagcgagctg gccggcgcgg cggcgcccgc gaacgtcgac ggccgcagcc tggtgccgct
     1321 cctgcgcgga cagaacgtcg cagactggcg aacggccgcg ctggtcgagc acctcggccc
     1381 ggccaacgat ccggccgacc cggacgtacc cgaaccccgc agcggcaacc cgacgacgta
     1441 cgacgcgctg cgcacgcgga cgtcactcta cgtcgagtac gccgacggcg agcgagagta
     1501 ccatgacctc gccgccgatc cagacgagtt gcgcaacacg tttgcgtcgc tgtccggccc
     1561 gcagaaggcg gcgttgagcg cgacggtcaa ggcgataaga aactgccacg gcgcgaacag
     1621 ctgctggccg gcggagcatg cgaaatgaat caccccagct gacgcccccg gcgcaggtcc
     1681 cgcctgtgga ttacgcaccg gtagggagcg tgtggtcctg gcggaagtct tctacccggc
     1741 aagagaggag tacctcaagt agaagtcttc ccaatcgggg accgctggac atccggaagt
     1801 tctgatgatc cgtcaaatat gattgtgctg gggcgctgcc gccgcaagcc aagaccgcgt
     1861 gtagagagcg cagggggaaa atcatgatga gaagagtgct tgccatcttg gggtccgcgc
     1921 tctttctcct catcgccgct ggtataggcg cgggggtcgt accgtggtgg atctcccggt
     1981 gggacatcca agcacctctc tttggctttc cgccgattcg tgtacttgga gtcctgctca
     2041 ttgtggcagg catcccggta ctgctggact cgtttgcacg ttttgcgctt caaggtatag
     2101 gcacgcctgc gcccgtgttt ccgacgcttc atctcgtggt caaggggttc tatcgttatg
     2161 tccgcaaccc tatttaccta gccgtcgtgg cggtaattct tggcgagggc gtgatcctcg
     2221 ggaacgtgaa cctccttgca tacggagtgc ttgcttggct gggcttttac ctatttgttt
     2281 tgggatacga ggagccaact ctgcggaaga cctttggggc cgagtacgac acattttgtg
     2341 ccaatgtacc tcggtgggtc cctcgtctga ctccatggag cgagagcgcc ggccggacgg
     2401 ccaatccagc acctcctccc cctcgcggct aatccttggc ggctgcgaga tgaaggcgat
     2461 ccttgctcgg aaacccctga taactatgat agaatggccg atgtggggcc tgtagctcaa
     2521 gggtagagca cccggctcat aaccggctga cccaggttcg agtcctggcg ggcccaccag
     2581 aacaaagcgt tccgtgtgat tctttcggct gctaccctcg tgctaccctc tcatcggccc
     2641 actgtcggca gggggcgccc
//
LOCUS       DASQGH010000215         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_966, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000215 DASQGH010000000
VERSION     DASQGH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..993)
                     /locus_tag="VEW91_04085"
     CDS             complement(22..993)
                     /locus_tag="VEW91_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYM90797.1"
                     /translation="MMGFLWPVALWGLASLPLFVAVYLWLLHRSRRAAVRFPQVDTLV
                     LAMATGHRFRRHILALLFLAALVAAIGGLARPVMRLPAPANLAGVMLAIDVSGSMLST
                     DVTPTRLEAAKAAAKAFVTGLPGDMRAGLVTFAGDAVLHVPPVTDHQRVAQVIDAIGV
                     RHRTAIGDGLVEAVAALPGRVRPLPDGSLPSPSQGSRPPGVVVLLSDGGNNSGVDPLQ
                     AADIARREVVTVYTIGIGRRPGEPASGWVIGGPVDEETLQAIARHTGGEYYHPSSAGE
                     MRAIYGKLARRVGWEIQPVEVTAALAALGAGALMAALVVSLRRPPLP"
     gene            1213..1827
                     /locus_tag="VEW91_04090"
     CDS             1213..1827
                     /locus_tag="VEW91_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90798.1"
                     /translation="MSRKLRTCLSGVVVLAVFLAPLAAGAARGPLKVGVVDFYAPTPL
                     APFSGFFPERFAADDLTDLLARAAGDRLVMIPRDAVQRSEQEMRWGEADVLHFARLRA
                     LAGAVGADRLVVGWIPQLTVEGGGGGRIGVFEGGDGASGASRAEVSLVIQVFDAGQGR
                     LVDEARYSASDVGGPRSELAKRVIDHALRPAIPALLDHLTAPGP"
     gene            1864..>2235
                     /locus_tag="VEW91_04095"
     CDS             1864..>2235
                     /locus_tag="VEW91_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYM90799.1"
                     /translation="MAAARVAIIAPGDMGHAVGARLRAHGARITTCLAGRSPRSARLA
                     HEAGMTAAADDTELVRTADIFLSIVPPAAAVAVAERIATALRGAGATPLYADCNAISP
                     ATADAIGRTITSAGARFLDVGI"
BASE COUNT          361 a          759 c          779 g          336 t
ORIGIN      
        1 ccggtacgag taggattcac ctcacggcaa gggcggcctc cggagcgaaa cgacaagcgc
       61 ggccatgagc gcgcctgcgc caagggctgc cagcgcagcg gtgacctcca cgggttggat
      121 ctcccatccc acccgcctgg ccagcttccc atagatcgcg cgcatttcac cggccgacga
      181 gggatgatag tactcgccac cggtgtgtcg ggcgatcgcc tgtaatgtct cttcgtccac
      241 cggcccaccg atgacccacc cgcttgccgg ctctcccggt cgccgcccga tgccgatggt
      301 atatacggtg acgacctcgc gccgagcgat gtccgcggcc tggaggggat ccaccccgct
      361 gttgttcccg ccgtcggaaa gcaacacgac aacccccggc ggccgggagc cctgcgacgg
      421 ggacggcagc gagccgtcgg ggagggggcg cacgcgcccg ggaagcgccg ccaccgcctc
      481 gaccagcccg tcgccgatgg cggtgcggtg cctgacaccg atcgcatcga taacctgcgc
      541 aacccgttga tgatcggtga ccggcgggac gtgcaggacg gcgtctcccg cgaacgtcac
      601 cagtccggct cgcatgtccc cgggaagccc ggtcacgaac gccttcgcgg ccgccttggc
      661 ggcctcgagg cgggtcgggg tcacatccgt ggacagcatg ctcccgctca cgtcaatggc
      721 cagcataacg ccggcgagat tcgcgggcgc gggaagccgc atcaccggcc gggcgaggcc
      781 gccgatggct gcgacgagcg ccgcgaggaa cagcagcgcc aggatgtgcc gcctgaagcg
      841 atgtcccgtt gccatcgcca gcacgagggt atcgacctgc gggaacctga ccgcggcccg
      901 gcgtgaacgg tgaagcagcc agaggtagac cgcgacgaag agcggtagac tcgcgagtcc
      961 ccaaagcgct actggccaaa gaaagcccat catgccccag cggacggaac caggagagcg
     1021 gatcggccgt gtatatagga ggggaacaca atccaaacct ccctaggggc ttcgcgtggg
     1081 ccacttcgat tcctgtcgac ctcgatcatc cgagttgggt gacaggccag tgatccgaga
     1141 aggggagcgc aggcgcgatg gcgtacgctg ggagcgggag gtacatcggt gatgctccgg
     1201 ggaagggtac ggatgtcccg aaagctccgc acgtgtctct cgggcgtggt ggtccttgcg
     1261 gtgtttttgg ctccgctggc tgcgggggct gcccgcgggc ctctcaaggt cggagtcgtc
     1321 gatttctacg cgcccacccc gctggcgccg ttctcggggt tctttcctga gcgcttcgcc
     1381 gccgacgacc tcaccgatct gctcgcccgg gcggcggggg atcggctcgt catgattccc
     1441 cgtgacgcgg tccagcggtc ggagcaggag atgcgctggg gcgaggcgga cgtcctccac
     1501 ttcgctcgcc tgcgcgctct ggccggcgcc gtcggtgccg atcgcctggt cgttggatgg
     1561 atcccgcagc tcacggtaga ggggggcggc ggcggcagaa tcggcgtgtt cgagggtggt
     1621 gacggagcca gtggtgcctc cagggcagaa gtatcattgg tgatccaggt gttcgacgcg
     1681 ggccagggca ggctggtgga cgaggcgcgg tattccgcct ccgacgtcgg ggggccccgg
     1741 tcggaactcg ccaaacgcgt catagaccac gccctcaggc cagcgatccc ggcgctgctc
     1801 gaccacctca ccgctccggg cccctgagat agcccgagaa aggacctcaa aagggggaac
     1861 cagatggcag cggccagggt tgcgatcatc gctccgggcg acatggggca cgctgtcggc
     1921 gcacgcctgc gggcccacgg tgcccgaatc accacctgcc tcgccgggcg aagtccccgc
     1981 tcggcgcggc tcgcgcatga ggccggcatg acggccgccg ctgacgacac cgagctcgtt
     2041 cgcacggcgg atatcttcct ctcgattgtc cctccggccg ccgcggtggc tgtggcagag
     2101 cgtatcgcaa cggcgctgcg aggcgcgggg gcgacgcctc tctacgccga ctgcaatgcc
     2161 atatcccctg cgaccgcaga cgcgatcggc cgcacgatca catcggccgg agcgcgcttc
     2221 ctggatgtcg ggatc
//
LOCUS       DASQGH010000216         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9422, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000216 DASQGH010000000
VERSION     DASQGH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9422"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VEW91_04100"
     CDS             <1..447
                     /locus_tag="VEW91_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYM90800.1"
                     /translation="ARGRWTTGMQFQGLAESGGAATMDARPEHGGGGTGPSPMETVLL
                     ALGGCTGMDVISVLQKMRAPLEGLEINITADRADEHPRVFTRIALDYVFVGAGLRPDQ
                     VGHAVELSQTRYCSVSAMLRRAAELTYSWRIVPAPGQRSGGPGGAG"
     gene            complement(452..1513)
                     /locus_tag="VEW91_04105"
     CDS             complement(452..1513)
                     /locus_tag="VEW91_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYM90801.1"
                     /translation="MDINELLVHMIARQASDLYVKPEAPPTLRIAGQFAPIGEDALSH
                     EEVGELAAQLMQPRHVKEFEERQQVNLAYYTPTLGRYRVNIYMQRSVPAIVIRRIRTD
                     IPTFEELRLPRAVADLALAPRGLVLVTGPAGSGKSTTLATMIDHRNRNIPGHIVTIED
                     PIEFMHTDQRSLVSQREVGTDCDSFAAALRDALRQTPDVILIGEMRDADSAEAAVHFA
                     ETGHLVLTTLHSTNASQAIERILHFFPAELHDDIQLQFSLNLIGVLSQRLVPRRDKDG
                     LVLALELMLATPHIRDLLRKGQIPQIRTAIQSGGQEGMQTFDQAVYALIKERLIDPET
                     GLQFADSPNDVRLKLRGLI"
     gene            complement(1516..2607)
                     /locus_tag="VEW91_04110"
     CDS             complement(1516..2607)
                     /locus_tag="VEW91_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYM90802.1"
                     /translation="MDITDLLVQTRDRGASDLHLTVGSKPTMRINGKLTRLPLSHLRR
                     EEIHTMLYDLLADEQKAQFEATHDLDFSLELGGIGRFRVNAFLGRLGEGVVLRLIPSK
                     IQTIDELGFPAILKDLAEEDQGLVLITGPTGAGKSTTLAAMVDHMNNTREDHVITIED
                     PIEFVHEHRKCNINQREVGPHTMSFSAALRAALREDPDVILVGEMRDLETIGLALTAA
                     ETGHLVLSTLHTNNAAQTISRIVDVFPPYQQEQIRIQLADSLLGVIAQTLVPTMDGST
                     RVAAMEIMVATPAIRNLIRENKVHQIPSAIQTGARDGMQSLDQSLKALVKTQKITNDE
                     ALRRAVEKQAFGQEISGPRGDFAGLKNQR"
     gene            complement(2607..4178)
                     /locus_tag="VEW91_04115"
     CDS             complement(2607..4178)
                     /locus_tag="VEW91_04115"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90803.1"
                     /translation="MTISGKVGKLGEILLATSLITSEQLNKAIAAQARTGKRLGQILV
                     EQGAATEDDVAWALSNQLMYPYIFLSRDIIDDEVVRLLPEAFLREHRVLPIQKFGQQV
                     TLAMADPTDQHTVDEVAAQTGLQVNRAVALASNIEETLRQLPSQQPRTPRRRPQGPEA
                     QYLQFHLAHALHEGATEIHFDQAGEAQHRVRYRLQGTLVDRAGHQGDLHAGLIRHLRD
                     FAGLADSPAAAAAVTVRIGEVEVRAMVSIVPATGGPAATIALHPYRTGVPDLVPLGIG
                     RKTLLVLRGALKGARGALVIGCADPLVRSTLIRAILPATHREEVWALETLPVYRHSAI
                     TQTAVISSAQAAPLLAGTFGASSGLVAVDDASHAATLVAAFERARVQRVVLGHPQDDT
                     VGLVAQVLETAGGALVASTLTGVLAARTVRLLCPQCKERVRPGSGAANGRHTFTPRGC
                     AACSFTGFRGYRVLTEVWLPGPKDRGRLRGGARETTLARFAQAAGPSMRAQGHALVDD
                     GLTSVEELSRVLEGF"
BASE COUNT          624 a         1411 c         1534 g          707 t
ORIGIN      
        1 gcgcgcgggc ggtggacgac ggggatgcag tttcaggggc tcgccgagtc ggggggcgcc
       61 gccacgatgg atgcccggcc ggagcacggg ggagggggga ccggcccctc gccgatggaa
      121 accgtgctcc tggcgctcgg cgggtgcacg gggatggatg tgatcagcgt cctccagaag
      181 atgcgggcgc cgcttgaagg gttggagatc aacattaccg ccgaccgggc cgacgagcac
      241 ccccgcgtct tcacccggat cgcgctcgac tacgtcttcg tcggggcggg actgcggccg
      301 gatcaggtgg gccacgcggt cgagctcagc cagacgcgct actgctccgt atcggcgatg
      361 ctgcgccggg ctgccgagct gacgtactcg tggcggatcg tgccggcccc ggggcagcgc
      421 agcggcggcc cagggggggc tgggtgaagg gttagatgag cccgcggagc ttgagccgca
      481 cgtcgttggg gctgtccgcg aactgcaacc ccgtctcggg atcgatcagg cgttccttga
      541 tcagggcgta cacggcctgg tcgaaggtct gcatgccctc ctgccccccg gattggatgg
      601 cggtccggat ctgggggatc tgccccttcc ggagcaggtc gcggatatgg ggggtagcca
      661 gcatcagctc gagggccagc accaacccgt ctttatcccg ccggggaacc agccgctggg
      721 acagcacccc gatcaggttc aacgagaact ggagctggat gtcgtcgtgc aattcggcgg
      781 gaaagaagtg caggatccgc tcgatggcct ggctggcgtt ggtcgagtgc agggtggtca
      841 ggacgaggtg tccggtttcg gcgaagtgga cggccgcctc ggcgctatcc gcgtcgcgca
      901 tctcgccgat caggatgaca tcgggggtct gccggagggc gtcccgaagg gccgcggcaa
      961 aggagtcgca gtcggtgccc acctcgcgct ggctgaccag gctgcgctga tcggtgtgca
     1021 tgaactcgat cggatcctcg atcgtgacga tgtgccctgg gatgttccga ttgcggtggt
     1081 cgatcattgt tgcaagcgtg gtggacttgc ccgatccggc cgggccggtc acgagcacca
     1141 gcccccgggg ggcgagggcg aggtcggcga cggcgcgcgg cagtcgcagc tcctcaaacg
     1201 tcgggatgtc ggtcctgatc cgccggatga caatcgccgg gacggaccgc tgcatgtaga
     1261 tgttcacgcg gtaccgaccc agggtcgggg tgtagtaggc caggttgacc tgctgccgct
     1321 cctcaaactc cttgacgtgc cgcggctgca tcagctgtgc cgcgagttcc cccacctcct
     1381 cgtgcgacag cgcgtcttcg ccgatgggcg cgaactggcc cgcgatgcgc agcgtggggg
     1441 gcgcctcggg cttgacgtag agatcggacg cctggcgggc gatcatgtgg acgagcaatt
     1501 cgttgatgtc cacgcctacc gctggttctt gaggccggcg aagtcgcccc ggggcccgga
     1561 tatctcctgc ccaaacgcct gcttctcgac cgcgcggcgc agcgcctcat cgttggtgat
     1621 cttctgggtc ttgaccagcg ccttgaggct ctggtcaagg gactgcatcc cgtcgcgggc
     1681 gccggtctga atcgccgacg gaatctggtg caccttattt tcgcggatga ggttccggat
     1741 cgccggggtc gcgaccatga tctccatcgc ggccacccgc gtactcccgt ccatggtcgg
     1801 cacgagcgtc tgcgcgatga cccccagcag cgagtcggcc agctggatgc ggatctgctc
     1861 ctgctggtac ggggggaaga cgtccacgat gcggctgatc gtctgcgccg cgttgttggt
     1921 gtgcagcgtc gataggacca ggtggcccgt ctccgccgcg gtgagcgcca gcccgatcgt
     1981 ttcgaggtcc cgcatctcac ccaccaggat gacgtcgggg tcctcgcgga gcgcggcccg
     2041 cagggcggcg gagaacgaca tcgtgtgcgg accgacctcg cgctggttga tgttgcactt
     2101 cctgtgctcg tggacgaact cgatcgggtc ctcgatcgtg atgacatggt cctcgcgcgt
     2161 attgttcatg tggtcgacca tcgccgcgag ggtggtggac ttgcccgcgc ccgtcgggcc
     2221 cgtgatgagc acgagcccct ggtcctcctc ggccagatct ttgaggatcg ccgggaagcc
     2281 gagctcgtcg atcgtctgga tcttggacgg gatgagccgg aggacgactc cttcgcccaa
     2341 ccgtcccagg aacgcgttca cccggaaccg gccgatgccg cccagctcca gcgaaaagtc
     2401 caggtcgtgg gtggcttcga actgggcctt ctgctcgtcg gcgaggaggt cgtagagcat
     2461 cgtgtggatc tcctcgcggc ggaggtggga aagcggaagg cgggtgagct tgccgttgat
     2521 ccgcatcgtc ggcttggacc ccacggtcag gtgcaggtcg gaggcccccc ggtccctggt
     2581 ttggacgagc agatcggtga tatccatcaa aacccctcca ggacccggga tagctcttcg
     2641 accgaggtca ggccgtcgtc cacgagcgcg tgcccctgcg cgcgcatcga tggcccggcg
     2701 gcctgggcga atcgggccag cgtcgtctcg cgcgcgccac cacgcagccg cccgcggtcc
     2761 ttgggaccgg gcagccacac ctcggtcagc acgcggtagc ctcgaaaccc cgtgaagctg
     2821 cacgccgcac acccccgcgg ggtgaacgtg tgccgcccgt tcgccgcccc ggagcccggc
     2881 cgcacccgct ccttgcactg cgggcagagc agccggaccg ttcgcgccgc gagcacgccc
     2941 gtcagggtgg acgcgaccag cgcacccccc gcggtctcca gcacctgggc gaccagcccc
     3001 accgtatcat cctgggggtg tcccaagacg acccgctgca cgcgcgcacg ttcgaacgcg
     3061 gcaaccagcg tggcggcgtg ggaggcgtcg tcgaccgcga cgaggccgga gctcgccccg
     3121 aacgttcccg cgagcagcgg ggcggcctgg gcggaagaga tgacggctgt ctgcgtgatc
     3181 gcggagtgcc ggtagaccgg cagggtctcg agcgcccaga cctcctcccg atgggtcgcc
     3241 gggaggatgg cccggatcag cgtggagcgc acaagtggat cggcgcagcc gatgaccagg
     3301 gcaccgcggg cgcccttgag cgctccgcgc agcaccagaa gggttttccg gccgatcccc
     3361 aggggcacga ggtcggggac cccggtgcgg tacggatgca gcgcgatcgt cgccgccggg
     3421 cccccggtgg cggggacgat ggagaccatc gcgcggacct cgacctcccc gatgcggacc
     3481 gtgaccgccg cggcggcggc cggagagtcc gcgagacccg cgaagtcccg caggtggcgg
     3541 atcaggccgg catggaggtc gccttgatgg cccgcccgat cgaccagggt cccctgcagg
     3601 cggtaccgca cccggtgctg cgcttcgccg gcctggtcga agtggatttc ggtggctccc
     3661 tcatgcaggg catgggccag atggaactgc aggtactgcg cttccgggcc ctggggccgg
     3721 cgcctcggcg tcctcggctg ctgggagggc agctggcgca gcgtctcttc gatattggac
     3781 gcgagggcca ccgcccggtt cacctggagc ccggtttgag cggcgacctc gtccaccgta
     3841 tgctggtcgg tgggatccgc catggccagg gtgacctgct ggccgaactt ctggatcggg
     3901 agcacgcgat gttcccgcaa gaatgcctcg gggaggagcc ggacgacctc gtcgtcgatg
     3961 atgtcccggc tgaggaagat gtaggggtac atgagctggt tgctcaacgc ccaggcgaca
     4021 tcgtcctcgg tggccgcccc ctgctcgacc aggatctgcc caagccgctt gccggtcctg
     4081 gcctgcgccg cgatcgcttt gttgagttgc tcggacgtaa tgagggatgt tgccagcagg
     4141 atctcgccca gtttcccgac ctttcccgat atcgtcaccc ggcacccctc ccggcgccgg
     4201 cccgtagctg ggccggcgtc ccacccgggg cacacatgcg agggcgcgga gtccggtgaa
     4261 cggtcggctc tccccc
//
LOCUS       DASQGH010000217         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20803, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000217 DASQGH010000000
VERSION     DASQGH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20803"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..834
                     /locus_tag="VEW91_04120"
     CDS             127..834
                     /locus_tag="VEW91_04120"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90804.1"
                     /translation="MDLRVSAGAFVALLGPSGCGKSTALRILADLEVPTEGRVLVEGA
                     PPAAARAHHRLAIAFQDPALLPWRTVYSNITLPLEVTRMNVSRGDVLDLIALVGLKGF
                     EGARPAQLSGGMKHRVAIARALVTRPRVLLLDEPFGALDEMTRQRLNLELLRIWSEQA
                     PTTLLVTHSIAEAVFLADRVVVMTPHPGEVRAEVPIELPRPRGPALLRSSAFHALSDR
                     LSELLFTGGPSGDGARG"
     gene            854..2479
                     /locus_tag="VEW91_04125"
     CDS             854..2479
                     /locus_tag="VEW91_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM90805.1"
                     /translation="MRPSTPAPQRDGRRTKGGLPDWADAVLGPAVLLAVWETLGTALP
                     WARYTLSSPSAIAFRAWHDLAIYPLNVVTTLSEAGRGFLWGNLVAIALALVIVQVPLI
                     EGLLMQVAVVIYATPLLAIAPIIAVVLPGDEPKVALAALAVFFPTLIASLVGLRSADR
                     AALDFIYTLGGRSWDALRKVRIPASVPWVLAGFRIAAPAAILGAILGEFLGGDRGLGI
                     AMVNAMATLDTPRVWGLAIAASLLAGLGFVLAATASRVLVTWAPPLDVAPASIARLGG
                     SAGRVRWAIRRTALIILSIGVIIGLWVAFIKIFHLNPFFAKDPAAVARDLIAGPQASE
                     RRSLLGGALATTLADAASGFCAGTVVASIVAMLFVVYPRIERAFMPVAITLRSVPVIA
                     MTPLLVLLFGRGILGTIMITGIITFFPTLVNVMQGLRSASPQAVELMALYNASPWAVI
                     WKVRLPSALPSLFASARISVLGSILGAIVAEWLATGRGLGYVMIIARAQFQLTILWAA
                     GILVTIVSVIIYGVIAWVEAPVLRRYAPSQADV"
     gene            2508..>3516
                     /locus_tag="VEW91_04130"
     CDS             2508..>3516
                     /locus_tag="VEW91_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM90806.1"
                     /translation="MGRAWSAEPAYDREALIGAAAGRLELELVLHGASVVNVFTGEVY
                     PADIGIFQGRIAHVTGPDDPPLTARTVRDRRGLWAIPGFIDTHVHIESSMVTPAHYAE
                     AVLPHGTTTVLIDPHELANVLGLRGVRYMLESSENIPLRILIMAPSCVPSAPGVETAG
                     ASFDRAEVEAMLAWPRVVGLAEVMDYPGVLKGDGRMMRLLEATDAARGLIQGHAPRLK
                     GRALSAYIAAGINSDHENREAAEALAKLRAGMYLEVRESSLSKNAAAMAEALRGRGYL
                     PTVTLCTDDVTPEDLLRHGHINHVARRLVEEGLSPVDVVRFGTLNAANRLRRHDLGAL
                     SP"
BASE COUNT          490 a         1194 c         1194 g          638 t
ORIGIN      
        1 ttcgggtgag cgtgttctga gttgagtatc gagccggagg cggagcgatc ccataccggc
       61 cttatcctag aaggtgtaag aaagacatac cggatcgacc ataaagaagt ccaggcgatc
      121 cagcgcatgg acctgcgggt ctcggcgggg gcgtttgtgg cgttgctggg cccgtcgggc
      181 tgcggcaagt cgacagcgct ccgcatcctg gcagatcttg aggtcccgac ggaagggcgc
      241 gtgttggtcg agggggcccc cccggcggcc gcccgggcgc atcaccgcct cgccatcgcc
      301 ttccaggacc ctgccctctt gccgtggcgc acggtataca gcaatatcac acttccgctt
      361 gaagtgacga ggatgaacgt ttcgaggggc gacgtgctcg atctcatcgc gctcgttggc
      421 ctcaagggct ttgagggggc gcgtccggca caactctcag gcggaatgaa gcaccgggtg
      481 gccatcgccc gcgcgctcgt gacgcggccc cgcgtcctcc tgctcgacga accattcggg
      541 gctctggacg agatgacccg ccagcggttg aatctggagc tcctccggat ctggagcgag
      601 caggcgccga ccacgttgct ggtcacgcac tcgattgccg aggcggtgtt tctcgccgac
      661 agggtggtgg tgatgactcc tcacccggga gaggtgcgcg ccgaggtgcc gatcgagttg
      721 ccgcgtcccc gtggaccggc cctgctccgc agcagcgcct tccacgcgtt gagcgatcga
      781 ctcagcgagc tgctctttac gggtggcccg tccggtgacg gggcccgggg ctgagatccc
      841 tgtagcggag accgtgcgtc ccagcacacc tgcaccccag cgcgacggcc gccgcacgaa
      901 aggcggtctg cccgactggg ctgacgccgt cctgggtccg gccgtcctgc tcgccgtatg
      961 ggagacgctc gggacggccc tgccctgggc gcgatatacg ctctcgtccc cctccgcgat
     1021 cgcgttccgc gcctggcacg acctggccat ctaccccctc aatgtggtca cgaccctgtc
     1081 ggaggctggg cgggggtttc tgtgggggaa tctcgtcgcg atcgccctgg ccctggtcat
     1141 cgtccaggtc cccctcatcg aaggtctcct catgcaggtc gcggtggtga tctacgcgac
     1201 ccccctcctg gcgatcgcgc cgatcatcgc agtggtcctt ccgggcgacg agcccaaagt
     1261 cgcgctggcc gccctggccg tcttcttccc caccttgatc gcctcgctcg tcggcctgcg
     1321 gtcggccgac cgcgcggccc tcgacttcat ctacacgctc ggcggtcgtt cgtgggatgc
     1381 gctccggaag gtccgcatcc ccgccagcgt tccgtgggtc ctcgcgggct tccgcatcgc
     1441 cgcccccgcg gccattctcg gcgcgatcct cggggagttc ctcggcggcg accgcgggct
     1501 ggggatcgcc atggtcaatg cgatggcgac gttggacact cccagggtgt ggggcctcgc
     1561 catcgcggcg agcctgctgg caggtctcgg gttcgtgctg gcggcgacgg cctcccgggt
     1621 gctggtcacc tgggcgccgc ctctcgatgt cgcgccggcg tccatcgcca ggctcggcgg
     1681 ttccgccgga agggtccgat gggcgatccg tcggacggcc ctcatcatcc tgtccatcgg
     1741 ggtgatcatc gggctgtggg tggcgttcat caagatcttt cacctcaatc cgttcttcgc
     1801 caaagaccct gccgccgtcg cgcgcgacct gatcgccggc ccgcaggcct ccgagcgccg
     1861 gagcctgctc ggcggggccc tcgcgacgac cctcgccgac gcggcgagtg gattctgcgc
     1921 cggcacggtg gtggccagca tcgtcgcgat gctcttcgtc gtgtacccgc gaatcgaacg
     1981 cgccttcatg ccggtggcga tcacccttcg gtccgttccc gttatcgcga tgacgccgct
     2041 gctcgtcctg ctcttcgggc gagggatcct cggcacgatc atgatcaccg ggatcatcac
     2101 gttcttcccc acgctggtca acgtgatgca ggggctgcgg tcggcctctc cgcaggcggt
     2161 cgaactcatg gcgctgtaca atgcctcgcc gtgggccgtc atctggaagg tgaggcttcc
     2221 cagcgcgctg ccgagtcttt ttgcgtcggc ccgtatctcc gtgctgggat cgatcctcgg
     2281 ggcgatcgtc gccgagtggc tcgcgaccgg gcggggcctc ggctacgtca tgatcatcgc
     2341 ccgcgcccaa ttccagttga ctattctctg ggcggccggg attctggtga ccattgtgtc
     2401 ggtgatcatc tatggggtga tcgcgtgggt cgaagccccg gtcctccgcc gctatgcgcc
     2461 tagccaggcc gacgtgtgag gaggcggacg tggagcgagg tgctggtatg ggaagagcct
     2521 ggagtgccga gcccgcctat gatcgggagg cgctgatcgg cgcggctgct ggccgactcg
     2581 agctggagct cgtgctgcac ggcgcctctg tggtcaatgt cttcacgggg gaagtctacc
     2641 cggccgacat tgggatcttc caggggagga ttgcccatgt caccggcccc gacgatcccc
     2701 ctctgaccgc ccgaacggtg cgggaccgtc ggggactctg ggcgatcccc gggtttatcg
     2761 atacgcacgt gcacatcgag agcagcatgg tgaccccggc gcactacgcg gaggccgtcc
     2821 tcccgcacgg gaccacgacg gtgctgatcg acccccatga gctggcgaac gtgctgggcc
     2881 tccgaggggt tcgctacatg ctggagtcct ccgagaacat ccccctgcgg atcctgatca
     2941 tggcgccgtc ctgcgtccca tcggccccag gtgtcgaaac cgcgggggcc agctttgacc
     3001 gcgccgaggt tgaagcgatg ctggcctggc ctcgggtcgt gggcctcgcc gaggtgatgg
     3061 actaccccgg agtgctcaaa ggcgacggcc ggatgatgcg cctgctggag gccaccgacg
     3121 ccgcgcgggg gctgatccag ggacacgcgc cccggctgaa aggccgggcg ctgtccgcgt
     3181 acatcgccgc gggcatcaat tccgaccatg aaaaccggga ggctgcggag gctctcgcga
     3241 aactgcgcgc cggcatgtac ctggaagttc gagagagctc cctgtcgaag aacgcggccg
     3301 cgatggcgga ggccctgcgg ggccgaggct acttgccgac cgtcacattg tgcaccgacg
     3361 acgtgacccc ggaagacttg ctccggcacg ggcacatcaa ccacgtcgcc cgccggctgg
     3421 tggaggaggg actgtcgccg gtcgacgttg tccgattcgg gacactcaac gctgcgaacc
     3481 gcctccggcg ccatgatctc ggggccttga gccctg
//
LOCUS       DASQGH010000218         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_15118, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000218 DASQGH010000000
VERSION     DASQGH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15118"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VEW91_04135"
     CDS             <1..467
                     /locus_tag="VEW91_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HYM90807.1"
                     /translation="DFDYYTGIVFEGHTAALGFPLLGGGRYDRLLEGFGAPSPATGFA
                     IRLDRVLAASTPPGEPAAAADIAVGFLEGSRAEALQLASALRARDLSVIVEILGRPWE
                     EMAEAAIAAGVPRAVLVAGSRAVVREGDGREHSMAIADLLARVADGGTLWIS"
     gene            464..1108
                     /gene="hisG"
                     /locus_tag="VEW91_04140"
     CDS             464..1108
                     /gene="hisG"
                     /locus_tag="VEW91_04140"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYM90808.1"
                     /translation="MTVAVASGRLLEESMRTLEAGGFLAAEAWRSTRRLILPCVDPHM
                     RVLIAKPADLLTYVEHGAADLGIAGKDMLLEQGRDVYELVDLGFGACRGVVALPEARA
                     AAWETLSPIRIATKYPRVTERYFWSRGRSVEIIEVNGTVELAPLVGLADGILDLVMSG
                     RTLAANHLVEVAEVFRSTARLVVNRVSLRTRAEAVQGVVERMRAAASDRSQSSV"
     gene            1124..2434
                     /gene="hisD"
                     /locus_tag="VEW91_04145"
     CDS             1124..2434
                     /gene="hisD"
                     /locus_tag="VEW91_04145"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYM90809.1"
                     /translation="MEIYRLGEVPAERMRQILRRSRAEVFASERMTHVRTIIDDVERR
                     GDAAVVEYTQRFDGVTLTPDRLRVDRQEVHRAYDAVDDGLRAALASSIERARRYNEWL
                     RPPGLALEEPEAGLTVGVKYTPVESVGVYVPAGKGAFPSTMITLVTPAVVAGVEEIAA
                     VLPPRKDGSADPAVLVAADLLGVSHVFRCNGVAGIAALACGTGTIPRVQLLGGPGNPY
                     VTAAQIAVQAYGVRLLSFLGPTESMILADESADPDRLALDLLNEAEHGADSAVILVTT
                     SMEVATEVRERLPRYLERLPAQRREFAEAALSRYGGAIVAGTVEEAVAFVNLYAPEHL
                     QIATRDPYATLGSVRNAGEILLGQHTPFSAANYAIGVPAALPTSGFARVTSGVTVLSY
                     LKATSVAHLNAAALERVRPVIERLGRYEGFPAHVLAVEARGGLS"
     gene            2431..3021
                     /gene="hisB"
                     /locus_tag="VEW91_04150"
     CDS             2431..3021
                     /gene="hisB"
                     /locus_tag="VEW91_04150"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HYM90810.1"
                     /translation="MSRVGRVVRATAETQVEAAWDLDGAGRFTGSTRLPFLDHMLAQL
                     ARHGRFDLSVHAKGDLEIDAHHTVEDVGVALGRALKDALGDGAGISRFASAHAPLDES
                     LVLAVVDVSGRPFLHYTVPVPRQRLGEYDTDLTEEFFRAFVMNAGITLHLILVHGRNA
                     HHIIEAAFKALALVLDRASRVDPRVPGVPSTKGTLA"
     gene            3018..3644
                     /gene="hisH"
                     /locus_tag="VEW91_04155"
     CDS             3018..3644
                     /gene="hisH"
                     /locus_tag="VEW91_04155"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYM90811.1"
                     /translation="MIAIVDYGAGNLRSIQKALERQGLEARITDAPRALDEAEALVVP
                     GDGAFGPAMERLRSAGFADRIRAFVDSGRPFLGICLGMQLLFDASEEDGVHRGLGVLP
                     GRVVRLSGGVKVPHMGWNALRLLRPSPLLDGCATGEYVYFIHSYHPVPADPGLVAATT
                     EYGGPVAAVVGRRNVWATQFHPEKSGAVGIRMVANFARWAVGAGASGR"
     gene            3622..4344
                     /gene="hisA"
                     /locus_tag="VEW91_04160"
     CDS             3622..4344
                     /gene="hisA"
                     /locus_tag="VEW91_04160"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HYM90812.1"
                     /translation="MLVLPAVDVRGGRCVRLIQGAADRERVYDGDPVTAARSWEAAGA
                     PWLHVVDLDGAFSGVPVNVEVIRRLIAAVRIPVEVGGGVRSFDAIERWIDAGAARVIL
                     GTAAVRDTTLLAEACRRFRDRIAVAIDARRGIVAAEGWVARTGLRAAAAATRAVRAGA
                     GRIIYTDIGRDGTFGGPNVAAIERMLRGVGAPVIASGGIASVKDIHRLRRLEPTGLEG
                     VIVGRALYEGRVRLEDLLAAAA"
     gene            4349..5107
                     /gene="hisF"
                     /locus_tag="VEW91_04165"
     CDS             4349..5107
                     /gene="hisF"
                     /locus_tag="VEW91_04165"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HYM90813.1"
                     /translation="MLARRVIPCLDVDAGRVVKGTSFVNLRDAGDPVELAVAYDQEGA
                     DEVVFLDITASHERRGIMEEVVRRTAEVLTIPFTVGGGLGTVEDLRRMLSAGADKVGI
                     NTAAVRRPELIREAALRFGSQCIVVAIDARQVSPERWEVYTHGGRTPTGRDAVGWARE
                     AADLGAGEILLTSMDQDGRGEGYDLALTRAVTRAVRVPVIASGGAGHPGHFVDAVREA
                     DADAILAASMFHYRRYSIAAAKAQLAGAGIPVRL"
     gene            5155..>5543
                     /gene="hisI"
                     /locus_tag="VEW91_04170"
     CDS             5155..>5543
                     /gene="hisI"
                     /locus_tag="VEW91_04170"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HYM90814.1"
                     /translation="MERKTLTFDARGLVAAVAQDAASGDVLMVAHMNREALERTLATG
                     QAWYWSRGRNRLWRKGEESGHTQRVSAIRADCDGDAILLKVDQTGAACHTGHRSCFYR
                     DVVRKGGRAAIGAETPPSRRKGGPGPRA"
BASE COUNT          741 a         1828 c         2156 g          818 t
ORIGIN      
        1 gggacttcga ttattacacc ggcatcgtct ttgaggggca caccgcggca ctggggttcc
       61 cgctcctggg cggaggacgg tacgaccggc tgctcgaggg gttcggggcg ccgtctccgg
      121 cgacggggtt tgcgatccgg ctcgaccggg tgctggccgc ctccaccccc cccggcgagc
      181 cggcggcggc cgccgacatc gcggtggggt tcctcgaggg cagccgcgcc gaggcgctgc
      241 agctggccag cgcattgcgg gcgcgcgacc tctccgtgat cgtggagatc cttggccggc
      301 cgtgggagga gatggccgag gcggcgatcg ctgcgggggt gccgcgggcg gtgctggtgg
      361 cgggaagccg ggcggtcgtg cgcgagggcg atggccgcga gcattccatg gcgatcgccg
      421 atctgcttgc ccgggtggcg gatggaggga cgctgtggat ctcatgaccg tggcggtcgc
      481 ctccggccgc ctgctcgagg aatcgatgcg gacgctcgag gctggaggat tcctggccgc
      541 ggaggcctgg cgatcgacgc gccggctgat cctgccctgc gtggatccac acatgcgggt
      601 gctgatcgcc aaaccggccg acctcctcac ctatgtggag cacggcgccg cggacctcgg
      661 gatcgccgga aaggatatgc tgctcgagca gggccgggac gtgtacgagt tggtcgatct
      721 gggattcgga gcctgccggg gcgtggtggc gctgcccgag gcgcgggccg cggcctggga
      781 gacgttgagc cccatccgga tcgcgacgaa gtacccgcgc gtgaccgaac ggtacttctg
      841 gagccggggg cgatcggtgg agatcatcga ggtgaacggc acggtcgagc tggccccgct
      901 ggtggggctc gccgatggca tcctcgatct ggtgatgtcc ggccgcaccc tggcggcgaa
      961 ccacctggtc gaggtggcgg aggtgtttcg ctccaccgcc cgcctggtcg tgaaccgggt
     1021 gagcctccgc acccgcgcgg aggccgtcca gggggtggtc gagcggatgc gggccgcggc
     1081 atccgaccgc tcgcagtcgt cggtgtagag gagagggagg cgcatggaga tctaccgatt
     1141 gggcgaggtg cccgctgagc ggatgcggca gatcctgcgg cggtcgaggg cggaggtgtt
     1201 cgcctccgag cgcatgaccc acgtgcggac cattatcgac gacgtcgagc ggcgcgggga
     1261 tgcggcggtg gtggagtaca cccagcggtt cgacggcgtc acgttgacgc cggaccgcct
     1321 gcgcgtcgac cgccaggagg tccaccgagc ctacgacgcg gtggacgatg ggctgcgcgc
     1381 ggcgctggcg agcagcatcg agcgggcccg gcggtacaac gagtggctcc ggccccctgg
     1441 gctggccctc gaagagccgg aagccgggct caccgtcggc gtcaagtaca cgcccgtcga
     1501 gagcgtcggg gtgtatgtgc cggccggcaa gggggcgttc ccgtcgacca tgatcacgtt
     1561 ggtcaccccg gcggtcgtcg ccggcgtcga ggagatcgcc gcggtgctgc cgccccgcaa
     1621 ggacggcagc gcggaccccg ccgtcctggt ggcggcggat ctcctcgggg tgtcacacgt
     1681 attccgctgc aacggcgtcg ccggcatcgc cgccctggcg tgcggaacgg ggacgatccc
     1741 ccgcgtgcag ctcctggggg gaccgggcaa cccgtacgtc accgccgccc agattgccgt
     1801 ccaggcctat ggggtccggc tgctctcctt cctcggcccg acggagtcga tgatccttgc
     1861 cgatgagtcg gccgaccctg accggctcgc cctggatctc ctgaacgagg ccgagcacgg
     1921 ggcggactcg gccgtgatcc ttgtcaccac ctcgatggag gtggccaccg aggtccggga
     1981 gcggctgccg cgctacctgg agcggctgcc ggcccagcgc cgcgagttcg ccgaggccgc
     2041 gttgagccgc tatgggggcg cgatcgtcgc cggcacggtc gaggaggcgg tggcgttcgt
     2101 caacctctac gcccccgaac acctgcagat cgccacccga gatccgtacg cgacgctggg
     2161 atcggtccgg aacgccggcg agatcctcct cggccagcac acgccgttct ccgccgccaa
     2221 ctacgcgatc ggcgtgcccg cggccctccc gacgagcgga tttgcgcggg tgacctccgg
     2281 ggtgaccgtc ctcagctacc tcaaggcgac gtcggtcgcc cacctcaatg ccgcggccct
     2341 cgagcgcgtg cggccggtca tcgagcgcct gggacggtac gaggggtttc ccgcgcacgt
     2401 cctggcggtc gaggcgcggg gaggcctgtc gtgagccgcg tgggccgggt cgtgcgggcg
     2461 accgcagaga cgcaggtcga ggcggcctgg gacctcgacg gcgcgggccg gtttaccggc
     2521 tcgacccgcc tgcccttcct tgaccacatg ctcgcccagc tcgcccgcca tgggcgcttc
     2581 gacctgagcg tgcacgccaa aggtgacctg gagatcgacg cccaccacac ggtggaggac
     2641 gtgggggtgg cgctcggtcg ggcgctcaag gacgccctgg gggacggggc cgggatcagc
     2701 cggttcgcct ccgcccacgc gcccctcgac gagtcgctgg tgctggccgt ggtcgacgtg
     2761 agcgggcgtc cgttcctgca ctacaccgtg ccggtgccgc ggcagcggct gggcgagtac
     2821 gacaccgacc tcaccgagga gttcttccgc gcctttgtca tgaacgcggg gatcacgctc
     2881 cacctcatcc tcgtccacgg ccggaacgcg caccacatca tcgaggcggc gttcaaggcg
     2941 ctggccctgg tgctcgaccg ggcctcccgg gtggacccgc gggtaccggg cgtgccgtcg
     3001 accaagggga ccctcgcatg attgcgatcg tcgactacgg ggccggcaac ctccgcagca
     3061 tccagaaggc gctggagcgc caggggctcg aggcccgcat caccgacgcg ccccgcgcgc
     3121 tcgacgaggc ggaggcgctg gtcgtcccgg gggacggcgc tttcggtccg gcgatggagc
     3181 gtctccgcag cgcagggttc gccgaccgga tccgggcgtt tgtcgattcc gggcggccct
     3241 ttctgggcat ctgcctcggc atgcagctcc tgttcgacgc gagcgaggaa gacggggtgc
     3301 accgggggct cggcgtgctg cccggccgcg tcgtccgcct ctcgggcggc gtgaaggtcc
     3361 ctcacatggg atggaacgcc ctgagattgc tccggccgtc ccccctgctc gatgggtgtg
     3421 cgaccggcga gtacgtgtac ttcatccact cgtaccatcc ggtcccggcg gatcccgggc
     3481 tcgtggcggc gacgaccgag tacggcggtc cggtggccgc cgtggtcgga cggcggaacg
     3541 tctgggccac ccagtttcac cctgagaaat cgggggcggt cgggatccgg atggtggcga
     3601 acttcgcgcg gtgggctgtc ggtgctggtg cttccggccg ttgacgtgcg cggcgggcgg
     3661 tgcgtccgcc tcattcaggg cgcggccgat cgcgaacgcg tctacgatgg cgaccctgtc
     3721 acggcagccc ggagctggga ggcggccggg gcaccgtggc tgcacgtggt cgacctcgac
     3781 ggcgcgttct cgggggtccc ggtgaacgtg gaagtgatcc ggcgtctcat cgcggccgtg
     3841 cggattcccg tcgaagtggg cggcggagtg cggagtttcg acgcgatcga gcggtggatc
     3901 gatgcgggcg cggcccgcgt catcctgggg accgccgcgg tcagggacac cacgctcctg
     3961 gccgaggcct gccgccggtt tcgggaccgc atcgcggtcg cgatcgatgc caggcggggc
     4021 atcgtggcgg ccgagggctg ggtggcccgc accgggctcc gtgcggcggc ggccgcgacg
     4081 cgtgccgtgc gggccggcgc cggccggatc atctacacgg acatcgggcg ggacgggacg
     4141 ttcgggggac cgaacgtggc cgcgatcgag cggatgctgc ggggggtcgg agccccggtg
     4201 atcgcctccg gcgggatcgc atcggtcaaa gacatccacc ggctccgccg ccttgagccc
     4261 acggggctcg agggggtgat cgtcgggcgg gcgctgtacg aggggcgggt gcggctggag
     4321 gacctcctgg ccgccgccgc gtgagccgat gctggcgagg cgcgtgattc cctgccttga
     4381 cgtggatgcc ggacgggtcg tgaagggcac gagcttcgtc aaccttcgcg acgccggcga
     4441 ccccgtcgaa ctcgccgtgg cgtatgatca ggagggcgcc gacgaggtgg tgttcctgga
     4501 catcacggcg agtcacgagc gccggggcat catggaggaa gtggtccggc ggaccgcgga
     4561 agtgctcacg atcccgttca cggtgggggg cggcctcggg accgttgagg acctgcggcg
     4621 gatgctcagc gccggggccg acaaggtggg gatcaacacc gcggcggtcc ggcggcccga
     4681 gctcatccgc gaagcggccc tgcggttcgg gagccagtgc atcgtggtgg cgatcgacgc
     4741 gcgtcaggtg tcaccggagc ggtgggaggt gtacacccac gggggacgga cgccgaccgg
     4801 acgcgatgcg gtcgggtggg cccgtgaggc ggccgatctc ggagccggcg agatcctcct
     4861 gacgagtatg gatcaggatg gacgcgggga ggggtacgac ctcgcgctca cgcgggcggt
     4921 cacccgggcc gtcagggttc cggtcatcgc ctccggcggg gcgggccatc ccggtcattt
     4981 tgtggacgcg gtcagggagg ccgacgccga cgccatcctg gccgcgtcca tgttccatta
     5041 tcgccggtac agcatcgccg ccgccaaggc gcaattggcg ggggctggaa tccccgtccg
     5101 cctgtgaagc catcgcggcc cgcacgcggc ggggtgggat ggggggttgg aggcatggag
     5161 cgcaagacgc tgacctttga cgcccgggga ttggtggccg cggtggcgca agatgccgcc
     5221 agcggagacg tgctgatggt cgcgcacatg aaccgggagg ccctcgagcg caccctggcc
     5281 accggacagg cgtggtactg gagccgcggc aggaatcgcc tgtggcgaaa gggagaggag
     5341 tccgggcaca cccagcgggt ctccgcaatc agggccgact gcgacggcga tgcgatactg
     5401 ctgaaggtgg atcagaccgg ggcggcctgc cacaccggcc accggtcctg tttctaccgg
     5461 gacgtcgtgc ggaaaggtgg ccgcgcggcg atcggcgcgg agacgccacc gtcacggcgg
     5521 aagggggggc ccgggccccg cgc
//
LOCUS       DASQGH010000219         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43359, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000219 DASQGH010000000
VERSION     DASQGH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43359"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VEW91_04175"
     CDS             <1..345
                     /locus_tag="VEW91_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90815.1"
                     /translation="VWTLAVPGRSPRRLINHNRLLARYPGADGVKTGYVHQSGLTLVA
                     SATHHGSRLIAVLLHSRNMYTDASRLLDYGFAHYHATPVVASDLRRDPPPFGFAAWVT
                     LLVVRFPPAPLL"
     gene            353..1249
                     /locus_tag="VEW91_04180"
     CDS             353..1249
                     /locus_tag="VEW91_04180"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HYM90816.1"
                     /translation="MPGPPWILRSLLFVPGHRAELIEKAPGYGPDALILDLEDGVAPE
                     SKPRARQAVAAALERGLAPGLAILLRVNAVGSGLLEEDLGAAFRPRVDAVCVPKCDTP
                     EQVHAIDAHLERHEDRWGVARGHTKLVPMIESAQGVLNAPAIARSVARVVALGFGAED
                     FTADIGVVRTREGGEVGYARAAVSLAAHAGRVEPLDGIYADFRDPAGLEADARAARAL
                     GYTGKMVIHPAQIDPVHAVFAPSPAEVEQARRVVSAFEHAQARGEGIAVVDGAMVDRP
                     VVLRAQRILEIAARPRSGADRG"
     gene            1246..1653
                     /locus_tag="VEW91_04185"
     CDS             1246..1653
                     /locus_tag="VEW91_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYM90817.1"
                     /translation="MMQGKRLFRSRDDSLLMGVCGGLGKYLGIDSNAVRLTFVVLAAW
                     NGLGILLYLATVLIVPEEPPPEPASVPPPPAQAQDETRRVRMLGWLLILAGGYLLVRG
                     HPLFRSVVQDSVFPVILILGGLVLLFQRSRRRG"
     gene            complement(1637..3163)
                     /locus_tag="VEW91_04190"
     CDS             complement(1637..3163)
                     /locus_tag="VEW91_04190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM90818.1"
                     /translation="MPRVPRPLLTGLLGLTAIRLALAATLPAGDDEAYYWEWSRHLAA
                     GYVDHPPAVAYLVWLAVRILGRTPFALHLVAVALAFATSLALWTLAREVLEREGAATW
                     AVVLFSIIPVFAAGSVLTAPDGPLFLCWVLTLLWTWRAANGGRAGAWLTAGVWCGLGL
                     QSKYAAVALPVSVGLWLLGSPTHRRWLARPEPYAGCALALAIFAPVVWWNATHHWVSF
                     TLTALGTSRWTEHGNFPFFIASQFIYLSPLMFPALIAGLAVAAYRWIARQDARWGFLA
                     AAGWPVVLGACAGSLLVWAKPHWSAPGFLAAVIAAAGIATERPPRGGVVLRWGAGAVL
                     GTAALLGAAFYALPFLTATVLSPRLDPAANYYGWAQAGPAIVAVAKRDARGPFFVASD
                     WYQVMAPFDFSTGGAVPATTITGDDQYRFWTRLDGLRGWDGLFIRDSRQTYDVDLGQV
                     CQSIEPGPSIPLVRGRVVMRTVDLVWCRHFAGRPVPRLGSFRWLPGVGRPGRQLTRAF
                     "
     gene            3225..>4099
                     /locus_tag="VEW91_04195"
     CDS             3225..>4099
                     /locus_tag="VEW91_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYM90819.1"
                     /translation="MRKDRGRWDARYAAGDRRHDVGPAPLLAQWVPRWSPGRALDVAA
                     GLGRHALLLAHHRWTVDAVDISLEGLRILRRRAQDAGLAVNLILADIGAFACRPAAYD
                     LIVNTFFLNRRLIPRYWRWLRPGGVLYFETHVATPNSPTRSHYGLRPGEARRLFARWE
                     ILDYAEGPAPDGSRIIDTARLVARRPPGHRSGRTPHRIGGAVAEGRTGLAVAIDGPMG
                     SGKSTVAREVARRLRFQYVDTGAMYRAIAIAAIRRGVAPDDAALGALARSVTLALEPL
                     PDGSARVLADGEDVTG"
BASE COUNT          582 a         1497 c         1476 g          544 t
ORIGIN      
        1 gtgtggacgc tcgccgtccc tgggagatcc ccccgccggc tcatcaacca caaccggctc
       61 ctggcccgct atccgggggc cgacggggtc aagaccggct acgtccatca gtcgggactg
      121 acgctcgtcg cctccgccac ccaccatggc tcgcggctga tcgcggtgct gctccacagc
      181 cggaacatgt acacggatgc gtcacggctg ctcgactacg ggtttgccca ctatcatgcc
      241 acccccgtgg tcgcctccga tctccgccgg gacccgccgc cgttcgggtt cgcggcctgg
      301 gtcaccctcc tcgtggtccg ctttcccccg gcgccgttgc tctgacccgc ccatgccggg
      361 cccgccctgg atcctgcgca gcctcctctt tgtgccgggt caccgggccg agctgatcga
      421 gaaagcgccc gggtacggtc cggacgcgct cattctggat ctcgaggacg gggtcgcgcc
      481 cgagagtaag ccccgggccc gccaagctgt ggccgcggcg ctggagcggg gcttggcgcc
      541 ggggctggct attctcctcc gcgtgaacgc ggtgggaagc ggcctcttgg aggaggacct
      601 cggtgccgcg ttccggcccc gtgtggacgc cgtgtgcgtg ccgaagtgcg atacccctga
      661 acaggtccat gcgatcgacg cccatctcga gcgccacgag gaccgatggg gggtcgcgcg
      721 gggacacacg aagctcgtcc cgatgatcga gagcgcgcag ggcgtcctga acgccccggc
      781 gatcgcccga tcggtggccc gggtggtcgc gctcggcttc ggggcggagg actttaccgc
      841 cgacatcggc gtcgtgcgga cgcgcgaggg cggggaggtg gggtatgcgc gggccgcagt
      901 gagcctggcc gcccacgccg gccgggtgga gccgctcgac gggatctatg ccgatttccg
      961 ggatccggca gggctagagg ccgatgcccg cgcggcgcgg gcccttgggt acacgggcaa
     1021 gatggtcatc caccccgccc agatcgatcc ggtccacgcc gtgttcgcac cgtcccccgc
     1081 agaagtggag caggcgcgcc gggtcgtgtc ggcgttcgag cacgcccagg cgcgcgggga
     1141 agggatcgcc gtcgtcgacg gcgcgatggt ggaccgcccc gtcgtcctgc gcgcccagcg
     1201 gatcctggag atcgccgcgc ggccccggtc gggcgcggac cggggatgat gcaggggaaa
     1261 cgcctcttcc gctcccgcga cgactcgctc ctgatggggg tctgtggcgg gctcggcaag
     1321 tacctcggga tcgacagcaa cgcggtccgg ctgaccttcg tcgtcctcgc cgcctggaac
     1381 ggcctcggga tcctgctcta cctcgccacg gtgctgatcg tgccggagga gccaccgccc
     1441 gagccggcgt cggtgccgcc accccccgcg caggcgcagg acgagacccg gcgggtccgg
     1501 atgctgggat ggttgctgat cctggcgggc ggctatctgc tcgtgcgggg gcaccccctg
     1561 tttcgctcgg tcgtgcagga ctcggtcttc cccgtgatcc tgatcctggg cgggctcgtt
     1621 ctcctgttcc agcgatctag aaggcgcggg taagctgccg cccaggccgt cccacccccg
     1681 ggagccagcg aaacgatccg aggcgcggca cgggccgtcc tgcgaagtgg cggcaccaga
     1741 cgaggtccac ggtgcgcatc acgacacgcc cgcgaacgag ggggatgctc ggccccggtt
     1801 cgatggactg acagacctgt cccaggtcga cgtcgtaggt ctgccggctg tcccggatga
     1861 acagcccgtc ccacccccgg aggccgtcca gcctggtcca gaaccggtac tgatcgtccc
     1921 cggtgatcgt cgtggccggg accgcgccgc cggtgctgaa gtcgaacggg gccatgacct
     1981 gataccagtc gctcgcgacg aaaaacggcc cacgcgcgtc ccgcttcgcc accgccacaa
     2041 tcgccggccc ggcctgcgcc cacccatagt agttggcggc ggggtcgagc cggggcgaca
     2101 ggaccgtggc cgtgaggaac gggagcgcgt agaatgccgc gccgaggagc gcggccgtcc
     2161 cgagcaccgc gccggccccc caccggagga ccaccccacc cctcggcgga cgctcggtgg
     2221 cgatcccggc cgcggcgatg accgcggcca ggaacccggg cgcggaccaa tggggtttcg
     2281 cccacacgag caggctcccc gcgcacgcgc cgagcaccac cggccacccg gcggcggcga
     2341 gaaaccccca ccgggcatcc tgccgggcga tccaccggta ggccgccacg gcgagcccgg
     2401 cgatgagcgc cgggaacatc agcgggctca ggtagatgaa ctgcgaggcg atgaagaacg
     2461 ggaaattccc gtgctcggtc caccgcgagg tgcccagcgc ggttagggtg aaggagaccc
     2521 agtggtgcgt ggcgttccac cacaccaccg gcgcgaaaat cgcgagcgcg agggcgcacc
     2581 cggcgtaggg ctcgggccgc gcgagccacc gccggtgggt gggggacccc aggagccaca
     2641 gccccaccga cacgggcagc gccaccgccg cgtacttgct ctggagcccc agcccgcacc
     2701 agacccccgc ggtcagccat gccccggccc gtccaccgtt ggccgcccgc caggtccaca
     2761 gcagggtcag gacccaacag aggaacagcg gcccgtccgg cgcggtcagg accgatccgg
     2821 ccgcgaacac cgggatgatc gagaacagca cgaccgccca cgtcgccgcc ccctcgcgct
     2881 cgaggacctc acgggcgagc gtccagagcg caagcgaggt cgcgaacgcg agggcgaccg
     2941 cgaccagatg cagcgcgaac ggggtgcgtc ccaggatccg gaccgcgagc cagacgaggt
     3001 acgccaccgc cggggggtgg tccacgtacc ccgcggcgag gtgccgggac cattcccagt
     3061 agtacgcctc atcgtccccc gccggaaggg tcgcggcgag cgccaggcga atggcggtga
     3121 ggcccaggag accggtgagg agaggccgtg gaacgcgcgg catgcctgga cgatggtacg
     3181 gaatcgtacc ccgcgccgtc aatcgcccgg ggtggcacac gcgcatgcgt aaggatcgcg
     3241 ggcggtggga cgcccggtat gccgccgggg accggcgtca cgatgtgggg cccgcgccgc
     3301 tgctcgcgca gtgggtcccg cggtggtccc ccggacgcgc gctcgatgtc gcggcgggac
     3361 tgggacggca cgcgttgctt ctggcgcacc accgctggac ggtcgacgcg gtcgatatct
     3421 ccctcgaggg gctgcgcatc ctccgccggc gcgcgcagga cgctgggctg gccgtcaacc
     3481 tcatcctggc cgacatcggc gcgttcgcct gccggcccgc ggcatacgat ctcatcgtga
     3541 acacgttctt cttgaaccgc cggctcattc cccgctattg gcggtggctc cggcccggtg
     3601 gggtgctcta cttcgagacg cacgtcgcca cgcccaactc gccaacccgg agccactacg
     3661 ggcttcgccc cggggaggcc cgccggttgt tcgcccgctg ggagatcctc gactacgcgg
     3721 aggggcccgc gcccgacgga tcccggatca tcgacaccgc ccggctcgtg gcgcgccggc
     3781 cccccggaca taggagcggc cggaccccgc accgaatcgg tggcgccgtg gccgaggggc
     3841 gcacgggtct ggccgtggcg atcgacgggc cgatggggtc gggcaagagc acggtggcgc
     3901 gcgaggtcgc gcggcgcctc cgcttccagt acgtggatac cggcgccatg tatcgcgcga
     3961 tcgccatcgc tgcgatccgc cggggcgtgg cgccggacga cgctgccctt ggggcgctcg
     4021 cccgctcggt gaccctggcc ctcgagccgc tgccggacgg ctcggcgcgg gtgctcgccg
     4081 acggcgagga cgtgaccgg
//
LOCUS       DASQGH010000220         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23647, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000220 DASQGH010000000
VERSION     DASQGH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23647"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VEW91_04200"
     CDS             <1..255
                     /locus_tag="VEW91_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90820.1"
                     /translation="IKEMREIQEVGETTRPGRAPLLKAFETNYLDKLDAAAKAKNWSQ
                     YSALVPSAVNACNQCHVKVDFAFIKYQIPSAPPAPLKMTP"
     gene            269..778
                     /locus_tag="VEW91_04205"
     CDS             269..778
                     /locus_tag="VEW91_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYM90821.1"
                     /translation="MNVSAPAGAAGPEDRRAFFTRLFWGLAALLGALVGIPVLGAFLS
                     PAFRRQERAQWIAIGPAGAFTGTPGLAHHVHPSREGWVNVSAEMQVWVAKTPDGTFRI
                     FDNHCTHLGCPYHWEATEQRFVCPCHNGVFDATGKVLGGPPPRPLDYYDSKVEAGTLF
                     MGAFHRGGT"
     gene            775..>1542
                     /locus_tag="VEW91_04210"
     CDS             775..>1542
                     /locus_tag="VEW91_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HYM90822.1"
                     /translation="MSGGWLRERLGGPLFGGFLNRTVPEETGLPHTLGSATLFLLVVQ
                     FVTGIVLAMNYAPSPEQAYDSITYIMGQVMFGPVLRGLHHWGSSALVILVGLHMLRVF
                     VWGSYKVPRELTWMVGVALFLLVMAFAFTGYLLPWTQRSYWATVVGTKMVGTVPAIGG
                     WLLQVVRGQADVGPVTLVRFYAIHVLLFPALLVPLVILHLYLVYRHGIAPAPGNEATV
                     RKTKRFHPDQLAEDLVVSLVILLALFALTAIWGIPTEV"
BASE COUNT          236 a          545 c          490 g          271 t
ORIGIN      
        1 atcaaagaga tgagggagat tcaagaggtg ggcgagacca ccaggcccgg gcgggcgccc
       61 ctcctcaagg cgttcgaaac gaactacctc gacaagctcg acgccgcggc caaggcgaag
      121 aactggtcgc agtattcggc cctcgtgccc tcggcggtca acgcctgcaa ccagtgccac
      181 gtcaaagtcg actttgcgtt catcaagtac cagatcccct ccgctccgcc ggcgccgctc
      241 aagatgaccc cgtagtcaca cgagcgggat gaacgtcagc gccccggccg gtgcggccgg
      301 cccggaagac aggcgggcgt ttttcacccg cctgttctgg ggcttggcgg ccctcctcgg
      361 tgccctcgtc ggcatcccgg tccttggggc gttcctgtcc cccgcatttc gtagacagga
      421 acgtgcccag tggatcgcca tcggtcccgc cggtgcgttc accgggacgc ctgggctcgc
      481 ccaccacgtg cacccctcgc gcgaagggtg ggtcaacgtc agcgcggaga tgcaggtctg
      541 ggtcgcgaag acccccgacg gaacgtttcg gatcttcgac aaccactgca cccacctcgg
      601 gtgcccctac cattgggaag cgaccgagca gcgcttcgtc tgcccgtgcc acaacggggt
      661 gttcgacgcc accggaaagg tcctcggcgg ccccccaccg cggccgctcg actactacga
      721 cagcaaggta gaagccggca ccctcttcat gggcgcgttt caccgcggag gcacgtgagc
      781 ggaggctggc tccgtgagcg actaggaggg ccgctgttcg gcgggttcct caatcgcacc
      841 gtcccggagg aaaccggact cccccacacc ctcggcagcg cgacgctttt tctcttggtc
      901 gtccagttcg tgaccggcat cgtcctggcg atgaactacg cgccgagccc cgaacaggca
      961 tacgacagca tcacgtacat catgggccag gtcatgttcg gtccggtgct gcgcgggctc
     1021 caccattggg gctccagcgc cctcgtgatc ctcgtcgggc tgcacatgct gcgggtgttc
     1081 gtgtgggggt cctacaaggt cccccgggaa ctcacctgga tggtcggggt ggcgctcttc
     1141 ctcctggtca tggccttcgc gttcaccggg tacctgcttc cctggacgca gcggtcgtac
     1201 tgggccaccg tcgtcggcac caagatggtc ggcacggtcc cggcgatcgg gggctggctc
     1261 ctgcaggtcg tgcgcgggca ggccgacgtg ggccccgtga cgctcgtgcg gttctacgcg
     1321 atacacgtcc tcctgttccc ggcgcttctg gtgcccctgg tgatcctcca cctgtacctc
     1381 gtctaccggc acggcatcgc cccggcgccc gggaacgagg cgacggtgcg caagaccaag
     1441 cgcttccatc ccgaccagct cgcggaggac ctcgtcgtct cgctcgtgat cctcctggcc
     1501 ctgttcgcgc tgacggcgat ctggggcatc ccgaccgagg tg
//
LOCUS       DASQGH010000221         6662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37889, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000221 DASQGH010000000
VERSION     DASQGH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37889"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VEW91_04215"
     CDS             complement(<1..966)
                     /locus_tag="VEW91_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90823.1"
                     /translation="MTGPEATTGAAPDGRPLLDVRHLRMYFPIQHGVMRRVVGHVRAV
                     DDVSFAIWEGETLGLVGESGCGKTTAARCILRAHTPTDGEILFRTAGGPTVDLSRLSR
                     GDLQPLRSQIQMIFQDPYGSLNPRMTILDIVGEPLLVNGMRNRRQRGERVEELLRLVG
                     LRPEYMQRYPHAFSGGQRQRIGIARALALNPRLVVADEPVSALDVSVQAQILNLMLDL
                     QRQFHLTYLFIAHNLSVVKHICDRVAVMYVGKIVELAATQELFRSPMHPYTAALMAAV
                     PVPDPRIRGGELELPGEVANPAAPPPGCYFHPRCPYAVDRCRTEPP"
     gene            complement(987..2072)
                     /locus_tag="VEW91_04220"
     CDS             complement(987..2072)
                     /locus_tag="VEW91_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90824.1"
                     /translation="MNDQTRPLLSVRDLRVYFFMDEGVVKAVDGISFDVLPGQVFGIV
                     GESGCGKTVAVRALLGIVEPPGRIVSGKILLRRPAGAPGVADGGAVSDLAALDPRGRT
                     MRSIRGGEIALIPQEPMAAFSPVHSVGNQILEAIALHRNVGERDNRRLAVQLCRDVGI
                     PAPEQNLDKYAWQLSGGLRQRALIAMALSCNPRLLIADEPTTAVDVTTQAQVLRLLRQ
                     LQASRGTAIIFITHDLGVIAQMADHVMVMYLGRVMEQGPVDRIFHAAQHPYTQALLRS
                     IPSIRSEPRTSLPTITGSIPHPFNRPRGCPFHPRCPHAIPGRCDQDTPALREIAGGHQ
                     VSCFLYEPPGGDRPAEGDAPGEGNPWP"
     gene            complement(2291..3412)
                     /locus_tag="VEW91_04225"
     CDS             complement(2291..3412)
                     /locus_tag="VEW91_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90825.1"
                     /translation="MAEERVSLASNWTLVWWRFKKHRLAMLSAGVLILFYLVVLFPDF
                     FSTQDPEATDARLAFIPTQHLALFEGLRFRPSVPAVTGHRNPITLRMEWRVDPARRIP
                     VRVFVRDYRYRMLGLVETDVHLLGTGPSPSAPGEAEAGVQSRVYLLGTDRLGRDQWSR
                     LMLGTRTSMTVGLAAVVLSVVLGAFLGGVSGYIGGWLDVVIQRLIELLQSLPTIPVWL
                     AMSAALPRDWPPQKVFFAISLILALVGWTTLGREVRGRFLSLREEDFVLAAELAGCSR
                     LRVIWRHMMPAMTSHIIATSTLAVPVLIISETSLSFLGLGIRPPAISWGVLLQEAQNI
                     QTVAQAPWLLIPGVVVIIAVLAFNLVGDGLRDAADPYGH"
     gene            complement(3492..4478)
                     /locus_tag="VEW91_04230"
     CDS             complement(3492..4478)
                     /locus_tag="VEW91_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90826.1"
                     /translation="MLRVLIQRLVLLPILLAVFSIVVFALVQAPPGGFLTSYLSTLAA
                     SGSSIDAAQVEALRRQFGLDQPFHVQYLRWVQNLLHGDLGLSLEYQRPNSELIRQYLL
                     LTVLLALFSFVLSWVIAIPAGIYSATHPHSTLDYVLTVVNYVGVAVPNFMLALVLMWI
                     AFAYFGLSITGLFSPEFEQAPWNPARVRDLLTHIWLPTIVLGIAGTARLTRIMRANLL
                     DELQKPYVVTARAKGRSEWRLIVKYPVRVAINPLVSTIGWYLPQLFSGSLIVATVMNL
                     PNIGPLLLHALVQQDMYLAGSVLMIYCFFTVVGTLISDLLLAWVDPRIRMER"
     gene            complement(4521..6482)
                     /locus_tag="VEW91_04235"
     CDS             complement(4521..6482)
                     /locus_tag="VEW91_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90827.1"
                     /translation="MNRRASGVVVLFVTAVVAVSLGAFGPTPTPGPASAASSSTYKEA
                     PALAELVKAGKLPPVAQRLPEDPLVVDTIEGIGQYGGVWRRGFLGPSDFNNVHRIMYD
                     ALVRYSPDGTKVEPKYASSVTPSTDFTTWTVKLRKGAKWSDGSPFGADDIMFWYSDVL
                     LNKDLTPALPVWMKNHDGSPVTVERVDDITVRWVYKEPNTTFLMELANKDSGDRQYAC
                     FLPAHYLKQFHAKYGDKAKLDQMVAAAKFKTWGELFASKAHPPENPQRPVMAAWVGVT
                     RVSDQLYLLRRNPYYVGVDRAGNQLPYIDTVQFKYFADAQSLNLAAIAGQLDEQERHI
                     ILGNYPVLKEHEKDGKYRVILWPGFGGADADVTFNETYTKDPDLGRLMANRDFRIAMS
                     YAINRSQIKESAFLGLGEARQPVAAPWSPYYPGDVYARKYTAYRPDEANRMLDRIGLN
                     QKDAEGFRLYPNSNRRITIEISVVPVFGPWPDVAQLIAKDWERVGIRTIVQVRERALH
                     FQMRQSDDLQAEIWNQDTAGFPFTGTPKYDPRTILYGAITIGPLWKQWYDTGGREGVE
                     PPAAAKRLVELIDKAKRDDPKTQARDAQEIFKIWVDNMFEIGTVGLTPMDQGVAVVNV
                     RMHNVPTKLGKDWPLRTPGNARTEQFYYK"
BASE COUNT         1099 a         2218 c         2295 g         1050 t
ORIGIN      
        1 cggcggttcc gttcggcagc ggtccacggc atacgggcag cgcgggtgga agtagcatcc
       61 cggcgggggc gcggcaggat tggccacctc gcctggcagc tcgagctccc cgcccctgat
      121 gcgcgggtcg gggacaggca ccgcagccat caacgccgcc gtatagggat gcatcggaga
      181 ccggaacagt tcctgggtcg ctgcgagctc cacgatcttc ccgacgtaca tgacggcgac
      241 ccggtcgcag atgtgcttga ccacgctcag gttgtgcgcg atgaacaagt aggtgaggtg
      301 gaactgtcgc tggaggtcga gcatgaggtt gaggatctgc gcctgcacag aaacgtcgag
      361 cgcggacacc ggttcgtctg caacgacgag gcgggggttt agggcgaggg cgcgcgcgat
      421 gccgatccgc tggcgctggc ccccgctgaa ggcatgcgga taccgctgca tgtactccgg
      481 gcgcaggccg accagccgca gcagttcttc gacccgctcg ccgcgttgcc gccggttcct
      541 catgccgttg acgagcagcg gttcccccac gatgtcgagg atcgtcatgc gcggattcaa
      601 cgagccgtag gggtcctgga agatcatctg gatctgggag cggagcggtt gcaggtcccc
      661 gcgggacagc cgcgagaggt cgacggtggg gccgccagcc gtccggaaca ggatttcgcc
      721 gtccgtcgga gtgtgggccc ggaggatgca ccgggcggcg gtcgttttac cgcagccact
      781 ctccccgacg aggccgagcg tctctccttc ccagatcgcg aaactgacgt cgtccaccgc
      841 gcgcacgtgc ccgacaacgc gccgcatcac accgtgctgg atcgggaaat acatccgcag
      901 atgtctgacg tcgagcagcg gccggccgtc gggcgcggct ccggtcgtgg cctcgggccc
      961 ggtcatggcg ccaggcgcgg ctccggtcac ggccacgggt tcccttcccc cggcgcatcc
     1021 ccctctgccg gcctgtcccc gccagggggc tcgtagagaa agcagctcac ctgatgcccg
     1081 ccggcgatct cccgcagggc gggtgtgtcc tggtcacacc gcccggggat cgcgtgcgga
     1141 cagcgcgggt gaaacgggca gccgcgcggc cggttgaacg ggtgcgggat ggagccggtg
     1201 atcgtcggca ggctggttcg gggctccgac cggatgctcg ggatggaccg gagcagcgcc
     1261 tgcgtgtagg ggtgctgggc cgcgtggaag atgcgatcga caggtccctg ctccatcacc
     1321 cgcccgaggt acatcaccat gacgtggtcc gccatctggg cgatcacgcc gaggtcgtgc
     1381 gtgatgaaga tgatcgcggt gccgcgcgat gcttgtagct ggcgcagcag gcgcaggacc
     1441 tgggcctggg tcgtgacgtc caccgccgtc gtcggctcgt cggcgatcag aagacgcggg
     1501 ttgcaggaca gcgccatggc gatgagcgcc cgctggcgca ggccgccgct cagctgccag
     1561 gcatatttgt ccaggttctg ctcgggcgcc gggatcccga cgtcgcggca caactggacg
     1621 gcgaggcgcc tgttgtcgcg ctcacccacg ttccggtgca gcgcgatcgc ctcgaggatc
     1681 tggttgccga ccgagtgcac ggggctgaac gccgccatcg gctcctgcgg gatcagcgcg
     1741 atctcgccgc cgcggatgct gcgcatcgtc cgcccgcggg ggtccagcgc ggcgaggtcc
     1801 gagaccgcgc cgccgtccgc aacgcccggc gcgccggcgg gccgccgcag caggatcttg
     1861 ccggacacga tgcgtccggg aggctcgacg atgccgagga gggcccgcac cgccaccgtc
     1921 ttgccgcagc cgctctctcc cacgatgccg aacacctggc ccggtaggac gtcgaaggag
     1981 atcccgtcga cggctttgac cacgccctca tccatgaaga agtagacccg gaggtcgcgt
     2041 acggacagca gcggtcgggt ctgatcgttc atgggcgggg gtaccgcatc cgccccgccg
     2101 tcctggtgca tcaccgcacg agatcggcaa cagcgggccg gacgtggcgg ccggtcccga
     2161 gcggatcctt cgaatccgtt cctcgacgcg acgtcatggc tcacgccgta ggcgagcgcg
     2221 gggacgcttt ggctcacgcc gcaggccccc gtcctgtggc cgaccgcctc gacacacagc
     2281 cggcgacgaa ttaatggcca tacggatcgg cggcgtcccg cagtccgtca ccgacgaggt
     2341 tgaacgcgag cacggcgatg atcacgacga ccccggggat gagcagccaa ggcgcctgcg
     2401 cgacggtctg gatgttctgg gcctcctgca gcagcacccc ccaactgatc gccggcgggc
     2461 ggatgcccag gcccaggaag ctgagcgacg tctcgctgat gatcagcacc gggacggcga
     2521 gcgtgctggt agcgatgatg tggctcgtca tcgcgggcat catgtgccgc cagatgacgc
     2581 ggagccggct gcagccggcc aactcggcgg cgaggacgaa gtcctcctcg cgcagcgata
     2641 ggaaccggcc gcgcacctcc cgcccgagcg tggtccagcc gacaagcgcc aggatcaggg
     2701 agatcgcgaa gaacaccttc tggggcggcc agtcccgcgg cagcgcggcg ctcatcgcta
     2761 gccagaccgg gatcgtgggc agggactgca gcagttcgat cagccgctgg atgaccacgt
     2821 cgagccatcc accgatgtat cctgagacgc cgccgaggaa cgcccccagg acgacgctga
     2881 ggacgaccgc ggccaggccc accgtcatcg acgtgcgcgt gcccagcatc aggcgggacc
     2941 actggtcgcg gccgagccgg tcggttccga ggaggtacac ccggctttgc actccggcct
     3001 ccgcctcgcc cggcgcggag ggggatgggc cggtacccaa gaggtggacg tccgtctcga
     3061 cgagccccag catccggtaa cgatagtcgc ggacaaacac gcggacgggg atgcgccgcg
     3121 ccgggtcgac ccgccattcc atgcgcagcg tgatggggtt ccggtgcccc gtgaccgccg
     3181 gcaccgatgg ccggaaccgc aggccttcga agagggccag gtgctgcgtc gggatgaagg
     3241 ccaggcgggc atcggtcgcc tcggggtcct gcgtgctgaa aaagtcggga aacagcacca
     3301 ccagatagaa gaggatcagc acgccggcgc tcagcatcgc cagacggtgc ttcttgaacc
     3361 gccaccacac gagcgtccag ttggacgcga gcgacacgcg ctcttccgcc acggaggccg
     3421 gtggtgcggc ggctgtggga acgccgccca ggtcgatggc gtgcccccgg gcttccgggt
     3481 ctactcccac gttaccgctc catgcggatc cgcggatcca cccaggccag caggagatcc
     3541 gagatcaggg tgccgacgac ggtgaagaag cagtagatca tcaggacgct gcctgccagg
     3601 tacatgtcct gctggacgag agcgtgcagc agcagcgggc cgatgttggg caggttcatc
     3661 accgtggcga cgatcaggct cccggagaag agttggggga ggtaccagcc gatcgtgctg
     3721 accagcgggt tgatggcgac gcgcaccggg tacttcacga tcagccgcca ctccgagcgc
     3781 cccttggccc gcgcggtcac gacgtacggc ttctggagct catcgagcag attggcccgc
     3841 atgatccgcg tgaggcgggc cgtcccggcg atgcccagca cgatcgtcgg caaccagatg
     3901 tgcgtgagca ggtcgcgcac gcgcgccggg ttccacggcg cctgctcgaa ctctggcgag
     3961 aagaggccgg tgatgctcag gccgaagtac gcgaacgcga tccacatcag gacgagcgcc
     4021 agcatgaagt tcgggaccgc gacgccgacg tagttgacga cggtcagcac atagtcgagg
     4081 gtcgagtggg ggtgcgtcgc cgagtagatg cccgccggga tcgcgatcac ccaggagagc
     4141 acgaacgaga agagcgccag caggacggtc agcagcaggt actggcggat cagctcgctg
     4201 ttcgggcgct ggtactccag cgacaggccg aggtcaccgt gcaggaggtt ctggacccag
     4261 cggaggtact gaacgtggaa cggctggtcg agcccgaact gccggcggag cgcctcgacc
     4321 tgcgccgcgt cgatcgacga gcccgacgcg gcgagcgtgg agaggtaact cgtgaggaag
     4381 cctccgggag gcgcctgcac cagcgcaaac acgacgatcg agaacaccgc gagcagaatc
     4441 ggcagcagca ccaggcgctg gattaggacg cgcagcatcg gactcggacc cccgcggtta
     4501 cctccggaga tgtgtcacga ctacttgtag tagaactgct ccgtgcgcgc gttccccggc
     4561 gtgcgcaggg gccagtcctt gcccagctta gtcgggacgt tgtgcatccg cacgttgacc
     4621 accgcgaccc cctggtccat cggggttagg ccgaccgtcc cgatctcgaa catgttgtcc
     4681 acccagatct tgaagatctc ctgggcatcc cgggcctgtg tcttgggatc gtccctcttc
     4741 gccttgtcga tcagctcgac gaggcgcttc gcggccgcgg gcggctccac gccctctctc
     4801 ccgcccgtgt cgtaccattg cttccagagc ggtccgatcg tgatcgcccc gtagaggatc
     4861 gtgcggggat catacttggg cgtccccgtg aacgggaagc cggcggtgtc ctggttccag
     4921 atctccgcct gcaggtcgtc cgactgccgc atctggaagt gcagggcgcg ctcgcggacc
     4981 tgcacgatcg tcctgatccc gacccgctcc caatccttgg cgatcaactg ggcgacgtcc
     5041 ggccaggggc cgaagacggg cacgacggag atctcgatgg tgatgcggcg gttgctgttc
     5101 ggatagaggc gaaagccctc ggcgtccttc tggttcaggc cgatgcgatc gagcatccga
     5161 ttggcctcgt ccggccggta cgccgtgtac ttccgggcgt agacgtcgcc cgggtagtac
     5221 ggggaccacg gcgcggctac cggctggcgg gcctcgccca gtcccaggaa cgcggactct
     5281 ttgatctggc tccggttgat cgcgtaggac atggcgatgc ggaagtcccg gttcgccatc
     5341 agccggccga ggtcgggatc cttggtgtag gtctcattga acgtgacgtc cgcgtccgcg
     5401 ccgccgaagc ccggccagag gatgacgcgg tacttgccgt ccttctcgtg ctccttgagc
     5461 acggggtagt tgccgaggat gatgtgccgc tcctgctcgt ccagctggcc ggcgatcgcg
     5521 gcgaggttga gggactgcgc gtcggcaaag tacttgaact ggaccgtgtc gatgtacggc
     5581 agctggttcc ccgcccgatc cacgcccacg tagtaggggt tgcggcgcag caggtagagc
     5641 tggtcgctga cccgcgtcac ccccacccag gccgccatca cgggacgctg ggggttctcg
     5701 ggtgggtgcg ccttggaggc aaagagctcc ccccacgtct tgaatttggc tgcggcgacc
     5761 atctggtcga gcttcgcctt gtccccgtac ttggcgtgga actgcttgag gtagtgggcc
     5821 ggcaggaagc acgcgtactg gcggtcgccg ctgtccttgt tcgccagttc catcaggaag
     5881 gttgtgttcg gctccttgta cacccaccgg accgtgatgt cgtccacccg ctccacggtg
     5941 acgggcgagc cgtcgtggtt cttcatccag accggcagcg ccggcgtcag atccttgttc
     6001 aggaggacgt cggagtacca gaacatgatg tcgtcggccc caaacgggct gccgtccgac
     6061 catttggccc ccttgcgcag cttgaccgtc caggtcgtga agtccgtgga cggcgtcacg
     6121 ctcgaggcgt acttcggctc gaccttggtc ccgtccggcg agtagcggac gagggcgtcg
     6181 tacatgatgc ggtggacgtt gttgaagtcg ctgggtccca ggaagccgcg gcgccacacc
     6241 ccgccgtact ggccgatgcc ctcgatcgtg tccacgacca gcgggtcttc aggcaaccgc
     6301 tgagccaccg gcggcaactt gccggctttg accagctcgg caagcgccgg cgcctccttg
     6361 tatgtggaag agctcgcggc cgacgccgga cccggcgtcg gcgtggggcc aaaggcgccc
     6421 aggctcaccg ctacgactgc cgtgacaaaa agcaccacca caccactagc tctgcggttc
     6481 atgctgtccc tcctcgtctc agaggccctg catcgcctca ccgggcagta cccggtacct
     6541 ctcatggtag gtactcgacg ggcagccgcg ttccttcacc tcctcgccag agccacggac
     6601 ccaaccgagg tcgctacaaa aacaccaaac cgcccaaaat cgcagaaggc cccccgagca
     6661 gg
//
LOCUS       DASQGH010000222         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_17984, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000222 DASQGH010000000
VERSION     DASQGH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17984"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VEW91_04240"
     CDS             <1..817
                     /locus_tag="VEW91_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYM90828.1"
                     /translation="VVLRAGPPHSGHLAFRVGRPDDLQTIEAYYARLRRPTRWVRDEE
                     AGQQLALRVQNELGVPFEFFYEIKREPRILQRFDQYRGAHIMRMDHFNLLVPDVAAGY
                     EAFRALGFRCSEYTVTDPPDVRLWAAWMYRKPGVHDVALMNGAGPRLHHLGLWVSDTV
                     SILRACDLLAAAGQGSAIERGPGRHGVSNAFFLYLRDPDGHRVELFTSDYYTGDPDFE
                     PIAWSVHDPRRGTFWGHAAPVSWFEEASTVLNLNGQVVETSRAQLRERPQTVT"
     gene            complement(865..2622)
                     /locus_tag="VEW91_04245"
     CDS             complement(865..2622)
                     /locus_tag="VEW91_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HYM90829.1"
                     /translation="MTATRNRSKWLRIGGGVLLVLVALLLVPFVVPVDRFRPLIVRLV
                     EENTGRKVEIEALRLRLLPTVHLQVVNLRVKHPGGFPPGDTLAVKSVDVGTTLGSLLS
                     RRPDVTYVAFNGVRVNLLESPGGATNYGIAGVGGRPANVNAAGPRNAPVFALARIDSV
                     AARNVEITSGVYDPRRGLVTPSFAVAGVNARVRGINVTAPDWMSGMEVAADLGGITVS
                     TPALSRPLRIQKGSFSFKAGEAKGTFAASLDTLSAVGVVTVANLKDPVADFEITVPEL
                     DVDRLRTLVAGGGAVSRGGTGAPRRLLARGAFKVARLAARPMEGGTATGRIRVYTDRV
                     EVDPYSLALYGGTVRGTAVLDSAAAHQPALVTAQVRGVDFGRVVKALAPAAKQQITGT
                     LEVDGRLALALGGDPLASLTGAGTFAVRNGTLPGLDMRNTLVALARVAQVNIPAGPTK
                     FQYFGGDFRIAQQRVYSDALRLEAEGLDAVARGSFGFNRTLDYTGTGHVKGSLGSAQP
                     QGQPGTPSSLLGNLLGGTLPGGAFDMRVSFSLKGTFDDPKFAPAGMPQLNQGTNPAQP
                     QRPAGPGNPFNLFPLPVGP"
     gene            complement(2953..>3142)
                     /locus_tag="VEW91_04250"
     CDS             complement(2953..>3142)
                     /locus_tag="VEW91_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90830.1"
                     /translation="IRHINTEYEGLENRVEAMNVVQAALHEMTTLHSMLPKRLKEKSW
                     VFLSLERGRTHYRSAGCK"
BASE COUNT          507 a         1114 c         1028 g          493 t
ORIGIN      
        1 cgtcgtgctg cgcgccggtc caccgcactc ggggcacctg gccttccggg tgggccgccc
       61 ggacgatctg cagacaatcg aggcgtatta cgcgaggctg cggcgaccga ccaggtgggt
      121 gcgcgacgag gaagccggcc agcagctggc gcttcgtgtc cagaatgaac tcggggttcc
      181 cttcgaattc ttctacgaga tcaagcggga gcccaggatc ctgcagcgct tcgaccagta
      241 ccgcggcgcc cacatcatgc gcatggacca cttcaacctc ctcgtcccgg atgtcgcagc
      301 aggctacgaa gccttccggg cactgggctt cagatgctcg gagtacacgg tgaccgatcc
      361 tcccgatgtg agactctggg ccgcctggat gtaccggaag ccgggggtcc acgacgtcgc
      421 gctcatgaac ggcgcgggcc cccgcctgca ccacctgggg ctctgggtga gtgacacggt
      481 cagcatcctg cgggcctgtg atctcctcgc cgccgccggg caggggtcgg cgatcgaacg
      541 ggggccgggc cggcacggcg tgagcaatgc gttcttcctc tacctccggg atcccgacgg
      601 gcaccgggtc gagctcttca ccagcgacta ttacacgggg gaccccgatt ttgagccgat
      661 cgcctggagc gtacacgacc cccgccgcgg caccttttgg ggccacgcag cgccggtgag
      721 ctggtttgag gaggccagca cggtcctgaa cctgaacggt caggtggtcg aaacctcccg
      781 tgcccagctc cgagaacggc cccagacggt cacctaggcg catcacgcgc cggcgcgggg
      841 cccgggaaga ccaaggcccg ccgcttacgg gccaaccggg agtgggaaga ggttgaacgg
      901 gtttccaggc cccgccggcc gctgaggctg cgccggattg gtgccctggt tgagttgcgg
      961 catgccggcc ggcgcgaact tgggatcgtc gaacgtgccc ttcagggaga acgacacccg
     1021 catatcgaag gcgccccccg ggagcgttcc gccgaggagg ttgcccaaga gtgacgacgg
     1081 agtgcccggc tgcccttgcg gctgagccga tccgaggctg cccttcacgt gccccgtccc
     1141 ggtatagtcc aaggtcctgt tgaacccaaa gctgccccgc gccaccgcgt ccagcccttc
     1201 ggcctccaac ctgagcgcat cgctgtacac ccgctgctgc gcgatgcgga agtcgccgcc
     1261 gaagtactgg aacttggtcg ggcctgcggg gatgttcacc tgcgcgaccc tggctaacgc
     1321 cacaagcgtg tttcgcatgt cgaggccggg caaggtgccg ttgcgcacgg cgaacgtccc
     1381 cgcaccggtg agcgacgcga gcgggtctcc tcccagcgcc agcgccaggc gaccgtcgac
     1441 ctccagcgtc cctgtgatct gctgcttcgc ggcgggggcg agggctttca ccaccctgcc
     1501 aaagtccacc ccccgcacct gggcggtcac cagagccggc tggtgcgctg ccgcagaatc
     1561 cagcacagcg gttccccgga cggtcccgcc atacaaggcc aggctgtacg ggtccacctc
     1621 caccctatcc gtgtacacac ggatccggcc ggtcgccgtt cccccctcca tcggccgggc
     1681 cgccagcctg gcgaccttga aggcgccccg tgccagcagc cgccgcggcg cgccggtgcc
     1741 cccccgcgac accgcgccgc cgccggcaac cagcgtcctt agcctgtcga cgtcgagctc
     1801 gggaacggtg atctcaaagt cggcgaccgg atccttgagg tttgccacgg tgaccacgcc
     1861 cactgcgctc agcgtgtcga gcgacgccgc aaaggtcccc ttggcctcgc ccgccttgaa
     1921 actgaagctt cccttctgga tccgcagcgg cctggacagg gccggcgtgg ataccgtgat
     1981 cccgcccaga tccgcggcga cctccatccc gctcatccag tccggcgcgg ttacattgat
     2041 gccgcgcact ctggcgttca ccccggcaac ggcaaacgag ggtgtcacca ggccacggcg
     2101 cgggtcgtag accccggacg tgatctccac attgcgggct gcgaccgaat cgatccgggc
     2161 gagcgcgaac acgggggcgt tcctgggtcc cgccgcgttg acgttcgccg gccttccccc
     2221 caccccggcg atgccgtagt tggtcgctcc cccggggctt tcgaggaggt tcacccggac
     2281 cccgttgaac gcaacgtagg tcacgtccgg ccggcgtgag agcaggctcc ccagcgtggt
     2341 gcccacgtcc accgacttca ccgccagcgt gtcccccggg gggaagccgc cgggatgctt
     2401 gactctaagg ttcaccacct gcaggtgcac ggtcgggaga agacggagcc gcagcgcctc
     2461 gatctcaacc ttgcgcccgg tgttttcttc cacgaggcgg acgatcagcg ggcggaagcg
     2521 atcgaccggc acgacgaacg gcaccagcag cagcgccacg agcaccaaca ggacgccccc
     2581 gccgatgcga agccacttcg accgattccg ggtggccgtc atagggcgat gggtaactcc
     2641 acggagtgtc cgtcgacgca gcggagcaag ttgtcctcat cgtagtcctc ggcccgctcg
     2701 ggtgcaagca cggccacccg ggcaggaaga tccgccgccg gaggtcgtcg ggcatcttcg
     2761 ggaacgcgtc ggtcggggag tcgaagtccc agtgggggga gtccgacgag gggtcagaag
     2821 cccaagtccc gttttgcgcg tcggcggaag atgaagtgga acgccagcgc cgagatcaga
     2881 aacgcgaggc tccagccggc tgcccgcggc acgctcggtg aggaacgctc ctccgctctc
     2941 aaccgatgca cctcatttgc accccgcgga tcgatagtgc gtgcgacctc gctccaggct
     3001 gaggaagacc cagctcttct ccttgaggcg cttcgggagc atgctgtgca gcgtggtcat
     3061 ctcgtgaagc gcggcctgca ccacgttcat cgcctcgacc cggttctcga gcccctcgta
     3121 ctcggtattg atgtgccgga tg
//
LOCUS       DASQGH010000223         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40611, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000223 DASQGH010000000
VERSION     DASQGH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40611"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VEW91_04255"
     CDS             complement(<1..739)
                     /locus_tag="VEW91_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018673596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM90831.1"
                     /translation="MERAPSQPLLRIASPALSYLAVAAVALVLWGYVVLPLFATVRES
                     AAGPHGLTLEQYRAFFSIEHPANIEALGTSLWISVLSVLTTGAVGVTLGFLLNRIEFP
                     GRRLIEIVSLVPLALPPLIGVLTFVFLYAESGIVPRGLQALFHLKESPFALRGLWGVI
                     VVHTFTMYPYFYLATATALAALDPSLEEAAGNLGASRLQVWTRVILPLLTPALVAGSL
                     LVFMVSMASYTAPLIFGVDRTMTMQIYI"
     gene            complement(742..1812)
                     /locus_tag="VEW91_04260"
     CDS             complement(742..1812)
                     /locus_tag="VEW91_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90832.1"
                     /translation="MTSVAVEHLSKTFAGGTTAVHDANLVVATGEFFTLLGPSACGKT
                     TILRMLAGFTLPSEGRILFGQRDVTHVPPNRRGTAMVFQNYALFPHLSVFENVAFGLR
                     IRRVSAGELTRRVGEVLGLVRLAGVEGRRIDQLSGGQQQRVALARALVVQPDVLLLDE
                     PLSNLDAKLREETRAEIRRIQTAAHITTVYVTHDQAEAMAISDRVGVMSAGRIHQIGT
                     PPEVYFRPKTRFVAEFIGKNNLVEGALVAASGASGTVDAGSGLRLEVPLDARPDRPPA
                     PGARVLIVIRPEAFRPAAPDETHNVLTGTVRRHEFGGAYTEYIVAASGHDWIVWLPRD
                     GIDAHPPGTLLRLAIAQLYMVA"
     gene            complement(1852..2910)
                     /locus_tag="VEW91_04265"
     CDS             complement(1852..2910)
                     /locus_tag="VEW91_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM90833.1"
                     /translation="MARRLRSTGWLAATVILLGLLLAGSTRTMGQAANTLIIYTARDR
                     AIFDYVVAKFEERYPEFRGNVQVLNMGAQQVLERIRAEKVNAQADIWWGGTQQAFQLA
                     SQEDLLQTINPPFASQIPAQDKAPNGTWFGEMLLPEMIMYNTQALKPNEAPQDWDDLI
                     DPKWKGKIIIRAVAPSGTMHTIFDAMIARFFKTDGNPNRGYDWLRKLDANTKEYAADP
                     TTLYLKIARQEGLVTVWDLQDILIQQEKQGLPFGYVMPKSGAPVLVDGVAEVKGAKHP
                     QAAERFLELLFSSEVRFALTAAYYQIPTIPLGSMPQPAWLKNLSLKAMDLPWDVMATH
                     DREWIQYWDQNIKGKGGR"
     gene            3118..>4594
                     /locus_tag="VEW91_04270"
     CDS             3118..>4594
                     /locus_tag="VEW91_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYM90834.1"
                     /translation="MNEGGLGNRELRDRYRAAILKPLFDAWKDALYPVLIEASRAHVV
                     ALTSGRVLRPDQAARLLEGLDTLGAMSPADLVYDPDIEDVYFFLERALAGRLGAEAAG
                     NLQLARSRNDLDAGAFRMVARAKLLAVVEASIAAGRAAAARARDGRDALVLARTHGQP
                     AQPTTLGHVLAAYAETVVRNLDRYRLAYAQVNCSPLGACALAGTSFALDRDELARLLG
                     FDGLVVNTYDAVVTADYALTALSACAIALSHDSRLLAMLEVWGTGPRPSVRMAPGFVQ
                     ISSMMPQKRNIVFVEHLRARATRAAGLLAGAAAGVAATPFEDDNRATSELQEDLWRTL
                     DDAAAVSRILDLALSSMAVGEGTPPEEIVASGATTTEVMDALVRRHGVSQRQAHAVVS
                     AMVERAPDPRTWTEAILAAVTAERLGRAVSFNTAEIQALLDPRTFVRTRVSAGGPAPG
                     QLDAELAAVIRALDGSERWAADARERLGAARAALRRRCAEIV"
BASE COUNT          710 a         1582 c         1584 g          718 t
ORIGIN      
        1 agatgtagat ctgcatcgtc atcgtccggt ccacgccgaa gatcaacggc gcagtgtagg
       61 acgccatcga gaccatgaac acaagcagcg acccggcgac cagcgcgggg gtcaggagtg
      121 gcaggatcac gcgcgtccag acctggagcc gactcgcgcc aaggttgccc gccgcctcct
      181 caagcgacgg gtcgagggcc gcgagcgcgg tcgcggtagc gaggtagaag tacggataca
      241 tcgtgaacgt gtgaacgacg atcacgcccc agaggccgcg tagcgcgaac gggctctcct
      301 tgaggtggaa gagcgcttgg aggccgcgcg gcacgatgcc gctctccgca tacaggaaca
      361 caaaggtgag gacgccgatc agcggtggca acgcaagcgg caccagcgag acgatctcga
      421 tcagccggcg gccaggaaac tcgatccgat tcagaaggaa cccgagcgtc acccccacgg
      481 ctcccgtggt caggacgctc agcacggaga tccagaggct ggttccgagg gcctcgatgt
      541 tcgcagggtg ctcgatcgag aagaacgccc ggtactgctc aagcgtgagg ccgtgcggcc
      601 cggcggcgct ctcgcggacc gtcgcgaaca gcggcaggac gacgtacccc cacagcacga
      661 gggccacggc cgcgaccgcc aggtacgaca gcgccgggga agcgatgcgc aggagcggct
      721 gggagggggc gcgttccacc ctcacgcaac catgtacagt tgtgcaatcg cgagccggag
      781 cagcgtcccg ggcgggtgcg catcgatgcc gtcccgcggc agccagacga tccagtcgtg
      841 cccgctggcg gctacgatgt actcggtgta cgcgccgccg aactcgtggc gtcgcaccgt
      901 gcccgtgagc acgttgtggg tttcgtcggg cgccgccggg cggaatgcct cgggacggat
      961 cacaatgagg acccgggcgc ccggcgccgg cggacggtcc ggccgcgcgt cgagcgggac
     1021 ctcgagccga aggccgctcc cggcgtccac ggtcccggac gctcccgagg cggcgacgag
     1081 tgcgccttcg acgaggttgt tcttgccgat gaactcggcg acgaaccgcg tctttggccg
     1141 gaagtagacc tccggaggcg ttccgatctg gtggatacgc ccggcggaca tgaccccgac
     1201 ccggtcggag atcgccatcg cctccgcctg gtcgtgggtc acgtacaccg tcgtgatatg
     1261 ggcggcggtc tggatccgcc ggatctcggc gcgtgtctcc tcgcgcagct tggcgtccag
     1321 gttggacagc ggttcatcga gcaacagcac gtcgggctgg acgacgagcg cacgtgcgag
     1381 cgccacgcgc tgctgctgac ccccggagag ttggtcgatc cggcggcctt cgacgccggc
     1441 gaggcgcacc agcccgagca cctcaccgac ccgccgcgtc agctcccccg ccgatacccg
     1501 ccggatcctg agcccgaacg cgacgttctc gaacacgctc aggtgcggga acagcgcgta
     1561 gttctggaac accatcgcgg tgccgcggcg gttcggcggc acgtgcgtca cgtcacgctg
     1621 gccgaacaag atcctcccct ccgaggggag ggtgaagccg gcgagcatcc ttaggatcgt
     1681 cgtcttcccg cacgcagacg gtcccagtag tgtgaagaac tctccggtcg caacgacgag
     1741 gttggcgtcg tggaccgcgg tcgtgccgcc ggcaaacgtc ttcgagaggt gctcgacggc
     1801 aacactagtc aggcgcgcgc ccctccctcg tcgcgcatcg tcgcggctcc gttaccgccc
     1861 gcccttgccc ttgatgttct ggtcccagta ctggatccac tcccggtcgt gagtcgccat
     1921 gacgtcccac ggcaggtcca tcgccttgag gctgaggttc ttgagccacg ccggctgcgg
     1981 catcgagccc agggggatgg tcgggatctg atagtacgcc gcggtcagtg caaagcggac
     2041 ctccgacgag aagagcagct ccaggaaccg ctccgccgcc tggggatgct tggccccctt
     2101 gacctccgcg accccgtcca ccaggaccgg cgcgccgctc tttggcatca cgtacccgaa
     2161 cgggagcccc tgcttctcct gttggatcag gatgtcctgg aggtcccaca ccgttaccag
     2221 cccctcctga cgggcgatct tcaggtaaag cgttgtgggg tccgccgcgt actccttcgt
     2281 gttggcgtcg agcttgcgca gccagtcgta cccgcggttc gggttcccat cggtcttgaa
     2341 gaagcgcgcg atcatcgcat cgaagatcgt gtgcatcgtc cccgacggtg ccaccgcccg
     2401 gatgatgatc ttgcccttcc acttgggatc aatcaggtcg tcccagtcct gcggcgcctc
     2461 gttgggcttg agcgcctggg tattgtacat gatcatctcg ggcagcagca tctccccgaa
     2521 ccacgtcccg ttcggggctt tgtcctgcgc cgggatctgg ctcgcgaacg gtgggttgat
     2581 cgtctggagc aggtcctcct gggaagcgag ctggaacgcc tgctgggtgc cgccccacca
     2641 gatgtccgcc tgcgcgttga ccttctcggc gcggatgcgc tccagcacct gctgggcgcc
     2701 catgttgagg acttgcacat tgccccggaa ctccgggtag cgctcctcga acttcgcgac
     2761 gacatagtcg aagatcgccc tatcgcgcgc ggtatagatg ataagcgtgt tggccgcctg
     2821 gcccatggtt ctggttgagc ctgcgagtag caggccgagc aggatcacag tggcggcgag
     2881 ccatcccgtc gagcgaagac gccgagccat cacaagccac cccctcgatc gtatcgtgcc
     2941 gtaccatgcg ttgcgcgtcg agccctcacc gcacccgacg gcgtagccgg ttttcgcgtg
     3001 tcggttcgtc ggccccttgc agaggttgct tgacaccatc agcaaggctc atgtaccatt
     3061 cgggtcggct ggcgcaacct cccgctgcgt ccgctgctcg aggataggtt cgtaggcgtg
     3121 aacgagggtg gcctcggaaa ccgggagcta agggaccgat accgcgcggc gatcctcaag
     3181 ccgctgttcg acgcgtggaa ggatgcgctg tacccggtgc tgatcgaggc gagccgagcg
     3241 catgtcgtcg ccctcacctc gggccgggtc ctcaggcctg atcaggcggc gcggctgctc
     3301 gagggcctcg atacgctcgg cgcgatgtcc cccgcggacc tggtgtacga tcccgatatc
     3361 gaggatgtgt acttcttcct cgagcgggcg ctggccggcc gcctcggcgc cgaggcggcc
     3421 gggaacctcc agctcgcgcg cagccggaac gacctcgacg cgggtgcctt ccgaatggtc
     3481 gcgcgcgcca agttgctcgc cgtcgtcgag gcgtcaatcg cggcgggccg ggccgccgcc
     3541 gcccgcgcgc gggacggtcg ggatgcgctg gtgctcgcgc gtacgcacgg gcagccggcc
     3601 cagccaacga cgttaggtca cgtcctcgcg gcgtatgccg agacggtggt gcgcaacctc
     3661 gaccggtacc ggctcgcgta cgcgcaggtc aactgctccc cgctcggcgc gtgcgcgctt
     3721 gcggggacga gctttgcgct cgaccgggac gagcttgcac ggctgctcgg gttcgacggg
     3781 ctcgtcgtga acacatacga cgccgtcgtc accgccgact acgcgctcac cgccctctca
     3841 gcgtgcgcga tcgcgctgag ccatgacagc cggctgctgg cgatgctgga agtgtggggg
     3901 accggcccgc ggccgtcggt ccggatggcc ccggggttcg tgcagatcag cagcatgatg
     3961 ccgcagaagc ggaacatcgt gttcgtggag cacctccggg cgcgcgccac ccgcgcggcc
     4021 gggctgctcg ccggtgcggc cgcaggcgtc gccgcgacgc cgttcgagga cgacaaccgc
     4081 gccacgtcgg aactccagga ggacctctgg cgcaccctcg atgatgccgc ggcggtgagc
     4141 cgcatcctcg acctcgcgct gtcctcgatg gccgtcggcg agggcacgcc cccggaggag
     4201 atcgtcgcgt ccggggcgac cacgaccgag gtgatggacg cgctggtccg ccgccacggc
     4261 gtctcgcagc gccaggcgca cgccgtcgtc tcggccatgg tagagcgcgc ccccgacccg
     4321 cggacgtgga ccgaggcgat cctggccgcg gtaacggccg agcggctcgg gcgggccgtg
     4381 tcattcaaca ccgccgagat ccaggcgctg ctcgaccccc ggacattcgt gcggacgcgc
     4441 gtgagcgccg gcggacccgc acccgggcag ctggacgcgg agctggccgc ggtcatccgg
     4501 gcgctcgacg gctcggagcg gtgggccgcg gatgcccggg agcgcctcgg cgccgcccgc
     4561 gcggcgctcc ggcgccggtg cgccgagatc gtcg
//
LOCUS       DASQGH010000224         7973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_998, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000224 DASQGH010000000
VERSION     DASQGH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..496
                     /gene="speD"
                     /locus_tag="VEW91_04275"
     CDS             14..496
                     /gene="speD"
                     /locus_tag="VEW91_04275"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HYM90835.1"
                     /translation="METVGHHNIVEGSGCNPEVLSRVEQVEQIMVRAAEVADVQVWAI
                     SFHRFSPNGVSGVVVISESHLSVHTWPEVGYVALDIYTCGDQAKPEAAVQHALKAFGA
                     SSMHITEVTRGLDEEDRVFFHSMITWEETIPDNILNHKPRRRRRITRRIRPAVAAKSP
                     "
     gene            complement(493..783)
                     /locus_tag="VEW91_04280"
     CDS             complement(493..783)
                     /locus_tag="VEW91_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HYM90836.1"
                     /translation="MDLVIINGVDALLTRAVQLLTLLVLVRVLLSWVPSVDYGHPLIM
                     LIVRITDPVLQPVRRLLPPMGGLDLSPVIAIVLLNLAGQLLHRFLLSLLAVG"
     gene            821..2056
                     /locus_tag="VEW91_04285"
     CDS             821..2056
                     /locus_tag="VEW91_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HYM90837.1"
                     /translation="MSCLRAGHPMTPRLYYTDAYLRVFTARIVAVEPRGSWGDRPGVI
                     LDQTAFYPTSGGQPHDTGTLAGIAVVDVVDADEAVVHVLEGVAGFPSVAAGAEVRGAV
                     DWERRFDHMQQHTGQHVLSAVFARVVGAETVSVHLDGSSTLDLAIPALSSEDARRVED
                     EANRVVLENRPVTVRFVAEEEAERLGLRRPPRRSGTIRVVEVEDCDRSACGGTHVRTT
                     GEIGPLMLRRWERTKGQIRVEFFAGWRALADYRWKHALVSEWSARLTVGDRELGAALE
                     RLAREARERERALSEARARLLTYEAAERLAAVPASSGRKILRLAVSGRDPNEVRQLVR
                     EMTGREACVVLVGDEATGRLYFARSPGDGPGMAAVIGHVCGAVGGRGGGTAEFAQGAV
                     PGESVAGVLARAEEVVRGA"
     gene            2049..2855
                     /locus_tag="VEW91_04290"
     CDS             2049..2855
                     /locus_tag="VEW91_04290"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYM90838.1"
                     /translation="MPELPEVEVARRSLRSAVLGKTVARTSVLRPVALRSHTPGQLDR
                     ALRGEKIADVQRRGKTLRFHLNGHVLVFHYMLWGVVRYHPGAEAPGAGTSLMLHFSDG
                     SCLEFRDLQLSRFHLVRAGAEGAGGEAAVEPLAPTTTFARFRAALPARGTIKNALSDQ
                     ARIAGIGNLWAHEILFAAGLKPARGTNTLRETDLRTLYRKMRQVLANAVRAGGEPDFE
                     DALGRRGRYRLMVYGRAGQPCRACGTRIRVGRLGGRPTFYCPSCQRDPGG"
     gene            2848..4035
                     /locus_tag="VEW91_04295"
     CDS             2848..4035
                     /locus_tag="VEW91_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HYM90839.1"
                     /translation="MAENPQIQVNAAAITGLLEKLVSIPSVNPTLVPGGAGEGEVARC
                     LARECERLGMTVAAEEAAPGRPNVVAVLPGVDPARGRSLLLNGHTDTVGAAGMAAPFT
                     PVRRGERLYGRGTSDMKAGLAAMVGAVAAVLDAGVRPRGDVLLTFAIDEEDASIGTAR
                     IARTRRADAAIVTEPTGLRICVAHKGFVWAAIRTQGRAAHGSDYAAGIDAILRMGRVL
                     QAVEQLDREVLSRRSHPLLGRPSVHASVITGGEGPSTYPPSCTLVLERRTLPEETAND
                     VRAELEAVLARLRGADPLFQATLEVTLARPGLEVDRDAAIVRSLHDAVARRLGTPPEY
                     VGLSAWFDAAVLAEAGIPTVIFGPTGAGWHAAEEYVDVPSVAACAEVLAQTIVDFCGV
                     GAA"
     gene            4037..4822
                     /locus_tag="VEW91_04300"
     CDS             4037..4822
                     /locus_tag="VEW91_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYM90840.1"
                     /translation="MRLLTFLSDFGSASPYPAAMKAAAAGICDAVLIDISHDVPPGSI
                     REGAYLLWSVAPTCPPGTVHCAVIDPGVGTGRAALAVSSGGQVFVGPDNGVLFPAARR
                     LGVPAIYRLNAPSFWRHPVSATFHGRDVFAPAAAHLASGTPVSTVGVPTKTHVDLVFP
                     PGRREGRVLVGEVLWIDRFGNLVTSIPGHMLAEVTPQTGLVVEVGSARLEAAPARTFG
                     DVASGQAAVLVGSDGMVEVAVNRGSAAHVLAASPGMALRIRPA"
     gene            5103..6458
                     /locus_tag="VEW91_04305"
     CDS             5103..6458
                     /locus_tag="VEW91_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYM90841.1"
                     /translation="MTRCRSALVALAVLVVLALAAPALPRAEAADAGFVLTTLETLRE
                     NYVDPVSAVPMLNAALTTLTQKTGVAPFDGLIPSGVSDVRAGVLFLPRFDELFSRVRE
                     RYSATDLAYTATAGMLESLHDSHTGFIPPEAYQEEKRKENGEAAFSGIGIVLMLRDGE
                     FFIREVFPQSPAAAAGLRPLDRLVGINGQPTAGRSSDEVSGAIRGPVGTTVVVGIERS
                     DAAGPFEVPVVRGPIRVPGVTSQMLDGGIGHLKIYEFVPGVGTGVRDAVFSLRRGGLR
                     ALVLDLRGNPGGLVDELRNIAAALLPQNSPILQMTNRNGKTVRMETLEPPILPGSVPI
                     VVLVDEGSASASELLASALQEQGRAAVVGVKTAGAVEIGITVDLPESAGMAVTVARLL
                     SGKGVRLEGRGVIPDELQSLETPALNLGHDSQFDRAVSLLKQQLGGKPSQPHRLVGVG
                     L"
     gene            6549..7133
                     /locus_tag="VEW91_04310"
     CDS             6549..7133
                     /locus_tag="VEW91_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYM90842.1"
                     /translation="MRGATVAAVIVVAAVVLIYGTFAGTYNRLVQANQSVNAAQADVE
                     TQLQRRFDLIPNLVESTRGVMAQERAVFEALATARTQYAGTRPGTPERVEAANQVQSA
                     LARLLVIVENYPVLRSSETVQSLMRELASTENQVAYARRGYNAAVQAYDTMVQTFPTN
                     LLASSFGFRTRPYFQAQPGSEQAPRVNLPVPAKP"
     gene            7135..>7973
                     /locus_tag="VEW91_04315"
     CDS             7135..>7973
                     /locus_tag="VEW91_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYM90843.1"
                     /translation="MRGRIVLALIAAAFALGAPVLAAGAATPAFPKPTGYVNDLAHLL
                     DPSARAALERRLAEYDRVTGNQIAVAIFPDLGGVPINEFAVRLEEAWKVGRRGKDNGI
                     LLLVGVRERQVRIEVGYGLEGRVTDSDAGMIIRNVIAPAFRAGRYGEGVDAAVGELIR
                     LSGGGGSPSAGVRVPRAARPIRLGWGLLPTLLFLVFVMLSSVFTVRSGMPRCPRCRTR
                     LVLQRGPGVSSVHRIDLWVCPQCGYSEKVLRQRSAAWVPAPMWLGGGAGWGTGGFSGG
                     GFGG"
BASE COUNT         1136 a         2700 c         2934 g         1203 t
ORIGIN      
        1 aggggggtga ccgatggaaa cggtaggaca ccacaacatt gtcgagggat ctgggtgcaa
       61 ccccgaggtc ctcagccgcg tggagcaggt cgagcagatc atggtccggg ccgccgaggt
      121 ggccgatgtg caggtgtggg cgatctcctt tcaccggttc agcccgaacg gcgtcagcgg
      181 ggtcgtggtg atctcggagt cccacctgtc ggtgcacacg tggcccgagg tcgggtatgt
      241 cgcgctggac atctacacct gcggggacca ggccaagccg gaggcggcgg tccagcacgc
      301 gctcaaggcc ttcggcgcct cgagcatgca catcaccgag gtcacccgag ggctggacga
      361 ggaggaccgg gtgttcttcc acagcatgat cacgtgggag gagacgatcc ccgacaacat
      421 cctcaaccac aaaccgcgcc gccgccggcg catcacccgc cggatcaggc cggccgtggc
      481 ggcgaagagc ccctagccga cggcgagcag tgagaggagg aaccggtgga ggagctggcc
      541 cgctaggttg aggagcacga tcgcaataac cggcgagagg tccagcccgc ccatcggcgg
      601 caaaaggcgg cgcaccggct ggaggaccgg gtcggtgatc cgcacgatca gcatgatcag
      661 cggatgcccg taatcgaccg aaggcaccca cgacagcagc acccgcacca ggaccagcag
      721 cgtgagcaac tggacggctc tggtcaaaag agcgtccacc ccgttgatga tcacgagatc
      781 cacgaaatta ttgtagcata gaagcggtgg acgggatcat gtgtcgtgcc tccgcgccgg
      841 gcacccgatg accccgcgcc tctattacac cgatgcgtac ctccgcgtgt tcaccgcgcg
      901 gatcgtggct gtcgagcccc gagggtcgtg gggcgaccgc ccgggcgtga tcctcgacca
      961 gacggccttc tatcccacct cgggcggaca gccgcacgat accgggaccc ttgccgggat
     1021 cgcggtggtt gacgtcgtcg acgccgatga ggcggtcgtc catgtcctgg agggcgtcgc
     1081 cggcttcccc tctgttgcgg cgggggccga ggtccggggc gccgtcgact gggagcgcag
     1141 gttcgatcac atgcagcagc acaccggtca gcacgtgctc tcggcggtgt ttgcgcgcgt
     1201 ggtcggggcg gagacggtct cggtgcacct ggacggatcc tccacgctgg acctcgccat
     1261 ccccgcgctg tcctccgaag acgcgcgccg cgtggaggac gaagccaacc gggtcgtgct
     1321 cgaaaaccgg ccggtgaccg ttcgattcgt cgctgaagaa gaggccgagc gcctggggct
     1381 ccgccgcccg ccgcgccggt cggggacgat ccgggtggtg gaggtggagg actgtgaccg
     1441 gtcggcgtgt ggcggcaccc acgtccgcac gaccggggag atcggccccc tgatgctgcg
     1501 gcggtgggag cggaccaagg gccagatccg cgtcgagttt ttcgccggat ggcgggccct
     1561 cgcggactac cggtggaagc acgcgctcgt cagcgaatgg agcgcccggc tcacggtcgg
     1621 cgaccgcgaa ctgggggcgg cgctggagcg gctggcccgt gaggcgcgcg agcgcgagcg
     1681 ggcgctctcg gaggcccgcg cccggctcct gacctacgag gccgcggagc ggctggccgc
     1741 ggtccccgca tcctcgggtc gaaagatctt gcgccttgcg gtgtctgggc gggatcccaa
     1801 cgaggtccgg caactggtgc gggagatgac cggccgcgag gcgtgcgtcg tcctcgtggg
     1861 agacgaggcg acgggacgcc tgtactttgc gcgttccccc ggggatggtc ccggcatggc
     1921 cgcggtgatc gggcacgtct gcggggcggt ggggggccgg ggcggcggca cggcggagtt
     1981 tgcccagggc gcggtgccgg gtgaatcggt cgcgggagtc ctcgcccgcg ccgaggaggt
     2041 cgtgcgcggt gcctgagctc cccgaggtcg aggtggcccg gcgcagcctg cgctcggccg
     2101 tcctcgggaa gaccgtcgcg cggacgtcgg tgctccgccc cgtcgccctc cgctcgcaca
     2161 cccccggcca gctggaccgc gcgctccgcg gggaaaagat cgccgatgtg cagcggcggg
     2221 gcaagacgct gcggttccac ctcaatggcc acgtgctggt gttccactac atgctctggg
     2281 gagtcgtccg gtatcatccc ggcgccgagg ccccgggggc gggcacgagc ctgatgctgc
     2341 atttttccga cggttcctgc ctcgaatttc gcgacctgca gctcagccgg ttccacctgg
     2401 tgcgggccgg tgcggagggg gcggggggag aggcggcggt cgaaccgctc gcgccgacca
     2461 cgacgtttgc gcggttccgc gccgcgctgc ccgccagggg gaccatcaag aacgccttga
     2521 gcgaccaggc ccgcatcgca gggatcggaa atctttgggc gcacgagatc ctgtttgcgg
     2581 cgggcctcaa accagcccgc ggcacaaaca cgctccggga gaccgacctg cgcaccctct
     2641 accggaaaat gcggcaggtg ctcgcaaacg cggtgcgggc agggggggag cccgacttcg
     2701 aagacgcgct gggacggcgc gggcggtacc ggctgatggt ctatggccgc gctggccagc
     2761 cgtgccgtgc gtgcggaacc aggatccggg tgggccggct cggcggcagg ccgaccttct
     2821 attgcccctc ctgccagcgg gaccccggtg gctgagaacc cgcagatcca ggtcaacgcc
     2881 gcggcgatca ccggcctgct cgagaagctg gtcagcatcc catcggtgaa cccgacgctc
     2941 gtgcctggag gcgcgggcga gggggaggtg gcgcgctgcc tggcgcgcga gtgcgagcgg
     3001 ctggggatga cggtggccgc ggaggaggcc gccccggggc gccccaacgt cgttgcggtc
     3061 cttcccggag tcgaccccgc gcggggccgc agcctgctct tgaacgggca cacggacacc
     3121 gtgggagccg cggggatggc cgcgccgttc acgccggtgc ggcggggcga gcgtctctac
     3181 ggccgcggca cgtccgacat gaaggccggg ctcgcggcga tggtaggcgc ggtggcggcg
     3241 gtcctggacg ccggcgtccg tccccggggg gacgtcctcc tgacgtttgc gatcgatgaa
     3301 gaggacgcca gcatcggcac cgcccgcatc gcccgcaccc gcagggcaga cgcggcgatc
     3361 gtgacggaac cgaccgggct tcggatctgc gtggcgcaca aggggttcgt gtgggcggcg
     3421 atccgcaccc agggccgcgc cgcccacggc agcgattacg ccgccggcat cgacgccatt
     3481 cttcgcatgg ggcgggtgct ccaggccgtc gagcaactgg accgcgaggt cctctcccgg
     3541 cgatcgcacc cgctgctcgg ccgtccgtcg gtgcacgcct ccgtcatcac gggaggggaa
     3601 ggccccagca cctacccgcc gtcgtgcacg ctcgtgctgg agcggcgaac gctgccggag
     3661 gaaaccgcga acgacgttcg cgcggaactc gaggcggtgc tcgcccgact ccgcggggcc
     3721 gacccgctct ttcaggccac gctggaggtc acgctcgccc ggccggggct cgaggtggat
     3781 cgcgacgccg cgatcgtccg ctcgctgcac gacgccgtgg cgcgccgcct gggaacccct
     3841 cccgaatacg tcgggctgag cgcgtggttc gacgcggccg tgctcgccga ggccgggatc
     3901 ccgacggtca tcttcggtcc caccggcgcc ggctggcacg ccgcggagga gtacgtggat
     3961 gtgccatcgg tggcggcctg cgcggaggtc ctggcccaga cgatcgtgga tttctgcggc
     4021 gtaggcgcgg cctagcgtgc gcctgctgac gttcctgtcg gacttcggca gcgcgtctcc
     4081 ctatcccgcc gccatgaagg ctgcggccgc tgggatctgc gatgcggtct tgatagacat
     4141 ctcgcatgac gtccctcccg gctcgatccg cgagggcgca tacctgctct ggtccgttgc
     4201 gccgacctgc ccccccggca ccgtccactg cgctgtgatc gatcccggcg tgggaaccgg
     4261 acgcgcggcg ctcgccgttt cctcgggcgg ccaggtcttc gtgggccccg ataacggcgt
     4321 gctgtttccg gccgcccgcc gtctcggcgt tcctgcgatc taccgcctca acgcgccctc
     4381 gttctggcgg cacccggtgt cggccacgtt ccatgggcgg gacgtgttcg cgccggcggc
     4441 ggcgcacctc gcctcgggga cgccggtgtc gaccgtcggg gtgcccacga agacgcacgt
     4501 cgacctggtg tttcccccgg ggcggcggga ggggcgcgtg ctcgtgggcg aggtgctgtg
     4561 gatcgaccgc ttcgggaatc tcgtgaccag catccccggc cacatgctcg cggaggtcac
     4621 ccctcaaacc ggcctcgtcg tcgaggtcgg ctcggcgcgt ctcgaagccg cgccggccag
     4681 gacatttggc gacgtcgcct ccggccaggc tgccgtgctc gtcgggagcg acgggatggt
     4741 ggaggtggcg gtgaaccgag ggagcgccgc ccacgtgctg gccgccagcc ccgggatggc
     4801 gctgcggatc cgcccagcct gaggagcccc aggcggtcgc gatctcggtc gaggaaggac
     4861 cccgaggatc ctccgccggt ccgacgaccg cgccgatcct gacggcgacg atctagagtg
     4921 gcgtccgcgc agcaggccgc agctcgggcg aacagggaat cgctgcgccc cggcgaacag
     4981 gctgatgcag ccctctgcgg ggtactatac acacggggga atgtgcacat cgccctccgc
     5041 gtttgacctt gctcacgggg ggcggtatgg cccccaagac cggagaggat gaacccgctt
     5101 ccatgacacg gtgtcgttcc gccctcgtcg cgctcgccgt tttggtcgtg ctggcgctgg
     5161 ccgctcccgc gctcccgcga gcggaggcgg cggacgccgg gttcgtgctc accacgctgg
     5221 agacgctgcg ggagaactat gtcgacccag tctcggcggt gccgatgctc aacgcggcgc
     5281 tgacgaccct tacccagaag accggtgtgg ccccgttcga cggcctgatc ccctcggggg
     5341 tgtccgatgt ccgcgccggc gtcttattcc tgccgcggtt cgatgagctc ttcagcaggg
     5401 tccgggagcg gtactccgcg accgatctgg cgtacaccgc aaccgcgggg atgctggaga
     5461 gcctccacga ctcgcacacc gggttcatcc cgcccgaggc ctatcaggaa gagaagcgga
     5521 aggaaaacgg tgaggcggcg ttttcgggaa tcggcatcgt gctcatgctg cgcgacggcg
     5581 agttcttcat ccgcgaggtg ttcccccagt cccccgccgc cgccgcgggc ctgcggccac
     5641 tggaccggct cgtgggaatc aacggccaac ccaccgccgg ccggtcgtcc gacgaggtca
     5701 gcggcgcgat ccgcggcccg gtggggacga cggtcgtcgt gggaatcgag cggtccgacg
     5761 cggcgggccc cttcgaggtc ccggtcgtgc gtggaccgat cagggttccg ggcgtcacca
     5821 gccagatgct cgacgggggg atcgggcatc tcaagatcta cgagtttgtc cccggggtcg
     5881 ggaccggggt ccgggacgcg gtcttttccc ttcgccgcgg cgggttgcgc gcgctggtcc
     5941 tggaccttcg ggggaacccc ggtggcctgg tcgatgaact gcgcaacatc gcggccgccc
     6001 tgctgccgca gaattccccc atcctccaga tgaccaaccg gaacggcaag accgtgcgga
     6061 tggagacctt ggagccgccg atcctcccgg gatccgttcc gatcgttgtg ctcgtcgatg
     6121 agggcagtgc ctcggcctcc gagctgctgg cgtccgccct gcaggagcag ggccgcgccg
     6181 cggtcgtcgg cgtcaagacc gccggggcgg tcgagatcgg gatcacggtc gatctgccgg
     6241 aaagcgccgg gatggcggtg accgtggcgc ggctccttag cggcaagggc gtgcggctgg
     6301 aggggcgcgg ggtgatcccg gacgaactcc aatcgctcga gacgcccgcg ctcaacctgg
     6361 ggcacgacag ccagttcgat cgggcagtga gcctgctgaa gcagcagttg ggtgggaaac
     6421 cgagccagcc ccatcggctc gtcggcgtcg gtctgtaggc cccgcgccgc gcgtgtcgcc
     6481 cgcggcagga cttgggtccc caccgccgta ttcagccacg gaaagtggaa ggggggagat
     6541 ctcggtccat gcggggggcg acggtcgcgg cggtcatcgt cgtcgcagcg gtggtgttga
     6601 tttacgggac cttcgccggg acgtacaacc gcctcgtgca ggccaaccaa tcggtgaacg
     6661 ccgcccaggc cgacgtggag acgcaactcc agcggcggtt tgacctgatc cccaacctgg
     6721 tggaatcgac ccggggcgtg atggctcagg agcgggccgt gttcgaggcc ctcgcgaccg
     6781 cgcgcaccca gtatgcgggt acccgccccg gcactccgga gcgtgtggag gcggcgaatc
     6841 aggtgcagag cgcgctggcg cgcctgctcg tgatcgtgga gaactatcct gtgctccgga
     6901 gcagcgagac cgtgcagagc ctgatgcggg agctggcctc cacggagaac caggtggcct
     6961 acgcccgccg cgggtacaac gccgcggtcc aggcgtacga tacaatggtg cagaccttcc
     7021 cgacgaacct cctcgcctcg agcttcgggt tccgcacccg gccctacttc caggctcagc
     7081 cgggatccga gcaggcgccc cgcgtcaacc tgcctgtccc tgcaaagccc tgacgtgcga
     7141 ggacggatcg tcctcgccct gatcgctgcc gcgttcgccc tcggcgcgcc ggttttagcg
     7201 gcgggggccg ccaccccggc gttccccaag cctaccggct acgtcaacga tttggcgcac
     7261 ctgctcgacc catccgcgcg cgcagcgctc gaacggcgcc tggccgagta cgaccgggtc
     7321 accgggaatc aaatcgcggt ggcgatcttc cccgacctcg gcggcgtgcc catcaacgag
     7381 tttgccgtcc ggctggagga ggcctggaag gtcggccgcc gggggaagga caacggcatc
     7441 ctgctgctgg tgggcgtccg ggaacgccag gtgcggatcg aggtcgggta cgggctggag
     7501 ggcagagtta ccgacagcga cgcgggaatg atcatccgga acgtgatcgc gccggcgttc
     7561 cgcgcagggc ggtacgggga gggtgtggac gccgccgtgg gggagctcat ccggctgagc
     7621 gggggtgggg gctcaccctc cgccggggtt cgagtcccgc gggctgcccg gccgatccga
     7681 ctgggttggg gcttgctccc gaccctcctg ttcctggtat ttgtcatgct gtcatccgtc
     7741 ttcaccgttc gaagcgggat gccccgctgc ccccggtgcc gcacccgcct ggtcctccaa
     7801 cggggccccg gcgtctccag cgtacatcgc atcgatctct gggtgtgccc ccaatgcggg
     7861 tacagcgaga aggtcctccg tcaacgcagc gcggcgtggg tgccggcgcc gatgtggctc
     7921 ggaggcgggg cggggtgggg gaccgggggg ttctcgggtg gtgggttcgg ggg
//
LOCUS       DASQGH010000225         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37903, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000225 DASQGH010000000
VERSION     DASQGH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37903"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(76..186)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(245..1243)
                     /locus_tag="VEW91_04320"
     CDS             complement(245..1243)
                     /locus_tag="VEW91_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HYM90844.1"
                     /translation="MTPLPIGKVPPALLGALVYTHLGAHRPDVLVHAQLGEDCATIEF
                     GDEVAVLTTDPITGAGEDRGWYAVFVATNDLAATGAEPVALALTLLLAPGRAEEDLSR
                     VMRDASAAASQLGVEIVGGHSEVTSGIDRTLIVMTALGRARKDRVLRSGGAVAGDALL
                     ITKGAGIEGTAILAAALEDRLRGALGERVVARAKALRDQISVLPEARAAAQSGARAMH
                     DVTEGGVLGAAYEMAAASGIGVRVDAARVPVPAETTAICGALGIDPLGLIGSGALLVA
                     APDAARTAGAIAAAGVQAQEIGRFVPRDRLVVRDGREMPLLPPGADELWRVLARSP"
     gene            complement(1278..2207)
                     /locus_tag="VEW91_04325"
     CDS             complement(1278..2207)
                     /locus_tag="VEW91_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYM90845.1"
                     /translation="MARLIRVGVVGLGQWGQHHVRVYHHLPGVLLAGVVDTSPSEVST
                     FARRYQTVGYRDHRMLFDKVDAVSIAVPTALHYEIAREFLQHGIHVLVEKPITTTVDQ
                     AAELVDLAQRAGVTLLVGHVERFKPSVQALTDMVTEPLFVQVRRVRPWDPTRVMDVGV
                     VLDLMIHDLDIILNLMKSPVARVSAIGAAIHGEDEDLAVAHLALRNGVLASFVASRVS
                     PVKAAELEITLPDGLIHLNYLRQQITVRRHGTQHRTVVKGGEPLRVELAHFVDCVRGM
                     AKPLVSGEHGLRALEVAHAILEKMSIITSRVAV"
     gene            complement(2218..>3170)
                     /locus_tag="VEW91_04330"
     CDS             complement(2218..>3170)
                     /locus_tag="VEW91_04330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYM90846.1"
                     /translation="ATSSGTAALEVALEAAGIRAGAGVVTTPFSFVSSTNAILRRGAV
                     PVFADIDPQTFNLDPDRVADTLTRTPGVQALLVVHLYGLPCRMDALMDLARHHDLVVI
                     EDAAQAHGAALQGRKVGTFGVAGAFSFYPSKNLTTGEGGMVVTSDAALAERARRLINV
                     GQSSQYTYELLGANYRMTEIAGALGIGQLARLEERNARRRQNAARLTAGLKDLDWLTV
                     PGEARGYSHVFHQYTLRVPAVRDRLARHLAARGIATRVYYPTPIHKSPLYRRLGFGEV
                     QCPQAEQAALEVLSIPVHPALGEEEIQRIVEAIHSFHPRH"
BASE COUNT          450 a         1145 c         1106 g          469 t
ORIGIN      
        1 caggtcactg atgtccagtt ttggcgtcat cgtcccccgc gccgccgcaa acgaaaagcg
       61 ccacccccgg aaccggggcg gcgcatcgcg tgtccctccg ctggcattac ccagatcagg
      121 ttccggggtc gatggcgggg ctagccatcc tctcagcctg gttccccaag ctcccccacg
      181 gctatgggtg gttcggctct agtataccac agcaccccct gttgggctgg cgcgccggcg
      241 tcggtcacgg tgatcgcgcg aggaccctcc acaactcgtc ggcgccgggg gggagaagcg
      301 gcatctcgcg gccgtcccgc acgaccaggc ggtcccgggg cacaaaccgc ccgatctcct
      361 gggcctggac cccggcggcg gcgatcgccc cggccgtccg cgcggcatcg ggcgccgcca
      421 ccaagagcgc cccgctgccg atcaggccca ggggatcgat cccgagggcc ccgcagatcg
      481 ccgtggtctc ggcgggcacc ggcacgcgcg cggcgtcgac ccggaccccg atgcctgacg
      541 ctgcggccat ctcgtaggcg gccccgagga cgccgccttc ggtcacgtcg tgcatcgcgc
      601 gggcgccgga ttgggccgcc gcccgcgcct ccgggagcac gctgatctgg tcccgaagcg
      661 ccttggcccg cgcgacgacc cgctcgccca gcgcgcccct caaccgatcc tcgagcgcag
      721 cggcgaggat cgccgtgccc tcgatcccgg cccctttggt gatcagcagg gcgtcgcccg
      781 ccaccgcgcc gccgcttcgg agcacccggt ccttccgcgc ccgtccgagg gccgtcatca
      841 cgatcagcgt gcgatcgatc ccgctcgtga cctcgctgtg gccgccgacg atctccaccc
      901 ccaactggct cgccgcggcg gacgcgtccc gcatcacgcg gctgaggtcc tcctccgccc
      961 ggccgggggc gagcagcagg gtgagggcga gcgcgacggg ctccgcgccg gtcgccgcga
     1021 ggtcgttggt cgccacgaac accgcgtacc agccgcgatc ctctcccgcg ccggtgatgg
     1081 ggtcggtcgt gaggacggcg acctcgtctc cgaactcgat cgtcgcgcag tcctcgccga
     1141 gctgggcgtg gacgaggaca tccgggcgat gcgccccgag gtgcgtatag accagcgcgc
     1201 cgaggagggc gggcgggacc ttgccgatgg ggagcggggt catgcgcggg cggaggcggg
     1261 gaggtcaggc tgcgggacta gacggccacc cgggaggtga tgatgctcat cttctcgaga
     1321 atggcgtggg ccacctcgag cgcgcgcagc ccgtgctcgc ccgaaaccag gggtttcgcc
     1381 atcccgcgca cgcagtcgac aaagtgggcc agttcgaccc gcagcggctc tccgcctttg
     1441 acgaccgtgc ggtgctgcgt gccgtgccgc cggaccgtga tctgctggcg gaggtagttg
     1501 aggtggatca gcccgtcggg cagcgtgatc tcgagttcgg ccgccttgac cggggagacc
     1561 cggctggcga cgaaactcgc gaggaccccg ttgcgcagcg ccagatgggc gaccgccagg
     1621 tcctcgtcct ctccgtggat ggcggccccg atcgcgctca cccgcgccac cggggacttc
     1681 atcagattga ggatgatgtc gaggtcgtgg atcatcagat cgagcacgac cccgacgtcc
     1741 atgacccggg tggggtccca cgggcggacc cgccggacct gcacgaagag cggctcggtg
     1801 accatgtcgg tgagggcctg caccgagggc ttgaaccgtt cgacgtggcc cacgagcagc
     1861 gtgacccccg cccgctgggc cagatcgacg agttcggcgg cctggtctac cgtggtggtg
     1921 atcggctttt ccacgaggac gtgaatcccg tgctggagga actcgcgggc gatctcatag
     1981 tggagcgcgg tcgggaccgc gatgctgacc gcatcgacct tgtcgaacag catccggtgg
     2041 tcccggtacc ccacggtctg gtaccgccgc gcgaacgtgc tgacttcgct gggcgaggtg
     2101 tccaccaccc ccgcgaggag gacgcccggc agatggtgat agacccggac atggtgctgt
     2161 ccccattgcc ccaggccgac gacgccgact cggatcagcc tagccacggg cacctcccta
     2221 gtgccgggga tggaagctgt ggatcgcctc cacgatccgt tgaatctcct cctctccgag
     2281 cgctgggtgg accgggatgc tcagcacctc cagcgccgct tgctcggcct gggggcactg
     2341 gacctctccg aaccctaacc ggcggtacag gggactcttg tggatcggcg tcgggtagta
     2401 gacccgggtc gcgatccctc gcgccgcgag atgccgggcc agccggtcgc ggaccgcggg
     2461 cacccgcagg gtgtattgat ggaagacgtg cgaatagccc cgcgcctctc ccggcacggt
     2521 gagccagtcc aggtccttca atccggccgt gagccgggcg gcgttctgcc gccgccgggc
     2581 gttgcgctcc tccagccggg cgagttgccc gatccccagc gctcccgcga tttcggtcat
     2641 gcggtagttg gccccgagca gctcgtaggt atactgcgag gactggccca cgttgatgag
     2701 ccgcctcgcc cgctcggcca gggcggcgtc gctcgtgacc accatcccgc cctcgccggt
     2761 ggtgaggttc ttggacgggt agaagctgaa cgcccccgca accccgaacg tcccgacctt
     2821 gcgcccctgg agcgccgcgc cgtgggcctg cgcggcgtcc tcgatcacga cgagatcgtg
     2881 atggcgcgcg aggtccatga gcgcgtccat ccggcagggc agcccgtaca ggtgcaccac
     2941 caggagcgcc tgcacgccgg gcgtccgggt cagcgtgtcg gcgacccggt cgggatcgag
     3001 gttgaacgtc tggggatcga tgtccgcgaa caccgggacc gcgccccgcc gcaggatcgc
     3061 gttggtcgag gagacaaatg agaacggcgt cgtcaccacc ccagcacccg cccggatccc
     3121 ggccgcctcg agcgcgacct cgagcgcggc ggttcccgat gaggtggcgg
//
LOCUS       DASQGH010000226         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3866, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000226 DASQGH010000000
VERSION     DASQGH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..356)
                     /locus_tag="VEW91_04335"
     tRNA            complement(281..356)
                     /locus_tag="VEW91_04335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(321..323),aa:Lys,seq:ctt)
     gene            446..535
                     /locus_tag="VEW91_04340"
     tRNA            446..535
                     /locus_tag="VEW91_04340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Leu,seq:tag)
     gene            656..1996
                     /gene="tig"
                     /locus_tag="VEW91_04345"
     CDS             656..1996
                     /gene="tig"
                     /locus_tag="VEW91_04345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYM90847.1"
                     /translation="MATTAVKMAWRTEPGSRAILEIDVPEDEVARAMDRAYAALAQRV
                     TVPGFRRGKAPRAVLERHVGTEALREEALKELLPERYAQAVEQAGLSPIGRPSFEVTE
                     GADGKGLRLTATVEVLPKIELPDYRTIRVTRDPHPVTDADVDRVLEDLRVRHGHLVSG
                     GSEPARRGDYVLLKVAKAPAGMGRLEPGKEVLVEIGGGLLPAEVEAALEGVRAGEDRE
                     VQPAGSEGVVELHVLDVRRKELPPLDDAFARVVSDEPTLASLRDRLRERLGRERAEAE
                     ERDLRERVLEAVIAPVTIDLPDSLVVHEVEHMLEDLTRRVRSRGLSLETYLRSQEKDE
                     AGLRADLRPGAERRVRVRFVLDEVARREGLTLTEEEMSRAVENLAQESREDVRKMQTW
                     LAQGDRLVGLREHLLRQKAMATLVACVSGDVAAGEAPPASAPGRTAEAPGAPVG"
     gene            2047..2649
                     /locus_tag="VEW91_04350"
     CDS             2047..2649
                     /locus_tag="VEW91_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYM90848.1"
                     /translation="MPIVVEQTARGERAYDIYSRLLRERLIFIGGPIEDEMANLIIAQ
                     MLYLEYEDPEKEIQLYVNTPGGSATAGLAIYDTMQYMRAPVSTICVGLAASAGAIILG
                     GGTKGRRFALSYSRMMIHQPWGGAQGTVSDISIQTKEFLAIRQILNEVLAKHTGQPLE
                     RVERDTERNFWMSAEEARDYGLIDDVIKSRKLAAPPAASR"
     gene            2653..3927
                     /gene="clpX"
                     /locus_tag="VEW91_04355"
     CDS             2653..3927
                     /gene="clpX"
                     /locus_tag="VEW91_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HYM90849.1"
                     /translation="MFKFGDERDRLKCSFCGKTQEQVRKLVAGPGVYICDECIELCNE
                     IIEEELYGEPHGQTKSKGTPKPQEIYQTLCQYVVGQDRAKKALAVAVYNHYKRIGSPP
                     KRATDVELQKSNILLIGPTGCGKTLLAQTLARVLDVPFAIADATSLTEAGYVGEDVEN
                     ILLRLIQTSDYDVKRAERGIVYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGT
                     TANVPPQGGRKHPHQEFIQIDTTNILFICGGAFDGLERIIESRTRVASIGFGAKIEQK
                     REQRMSELLGQVMPQDLLRYGLIPEFIGRLPIVVPIEPLAESDLVTILVEPRNALVRQ
                     YQKILEMDGVELVVTDGALRAIAREAMSLHTGARGLRTVLEEIMLNIMYEIPSRGDVK
                     RCVLAEETVSKRIEPLLLTASDLKKVPKDKPA"
BASE COUNT          789 a         1321 c         1472 g          634 t
ORIGIN      
        1 cccgtgccct aatctcatgg cttatagcca ttttcactcg gctaggcaac aagcagatcc
       61 ctcacgaacc ggacatctcc gaggcgttcc acgagaccct caagggggca ggcgtgacga
      121 ggatccgctt ccacgactgc cgccacgtct tccgccacgt cttccgtccc tcttgatcgc
      181 agcgggcaag aacgtgaagt acatctccac gcagatgggc cgccactcgt ccgaagggcg
      241 ggtttccagc gatctcgtgc atccggtgca agggagtgcg tggtgggccg tgcaggactc
      301 gaacctgcaa ccttgggatt aagagtcccc tgcactacca gttgtgctaa cggccccgga
      361 gaaattgtag caaaggccaa ggggggtgta aacgcgaacg gccgttgcgc gccgcggcaa
      421 agggacgtag aatcaaaacg atcgcgcggg cgtggcggaa cccggcagac gcaccaggct
      481 taggacctgg tggatcgaaa ggtccgtggg ggttcaaatc cctccgcccg caccagggtg
      541 tcgcggtttg cttgaacccc ccgagcgtcg tgtgctatac tgcgcgtgaa ccgatagcct
      601 cggcggcgca cccgtcgggg ctatctgccg tcagatgtga ggtgcgcgta cccgcatggc
      661 aacgaccgcg gtgaagatgg cgtggaggac ggagccgggc agccgcgcga tccttgagat
      721 cgacgtcccc gaggacgagg tggcccgggc gatggaccgg gcgtatgccg cgctcgcgca
      781 acgggtgacg gtgccggggt tccggcgggg gaaggcccca cgcgcggtgc tggagcgcca
      841 cgtcggcacg gaggcgctcc gagaggaagc gctcaaggag ctccttccgg agcgctacgc
      901 gcaggccgtc gagcaggccg gtctctcccc gatcggccgc ccctcgttcg aggtcacgga
      961 gggcgctgac ggcaaggggt tgcggttgac tgccacggtg gaagtcctgc ccaagattga
     1021 gctccccgat taccggacga tccgggtcac ccgcgaccct cacccggtga ccgatgccga
     1081 cgtggatcgc gtgctcgaag acctgcgggt ccggcacggg catctcgtga gtggggggag
     1141 cgagcctgcg cgccgcggcg attacgtgct gctcaaggtg gcaaaggctc cggcaggcat
     1201 ggggcgcctg gagcccggga aggaagtcct cgtcgagatc ggcggtggcc tgctgcccgc
     1261 cgaggtggag gctgctctcg agggcgtccg ggcgggggaa gaccgggagg tccagccggc
     1321 ggggagcgag ggggtggtcg agctccacgt gctcgacgtc cgccggaagg agttgccccc
     1381 cctcgatgac gccttcgccc gggtggtctc cgacgagccg acgctcgcca gcctgcgcga
     1441 ccggctgcgg gagcggctgg gtcgcgagcg ggcggaggcc gaggagcggg atctgcggga
     1501 gcgcgtgctc gaggcggtga tcgcgccggt gacgatcgat ctgcccgaca gcctggtggt
     1561 gcacgaggtc gagcacatgc tcgaggacct cacccgccgg gtgcggtcca ggggattgag
     1621 cctggagacc tatctgcggt cgcaggaaaa ggatgaagca ggactccggg ccgacctgcg
     1681 gccgggcgcg gagcgccggg ttcgggtgcg attcgtgctg gacgaggtgg cgcgccgcga
     1741 gggactgacg ctcacggagg aggagatgtc tcgcgcggtc gagaacctcg cccaggaatc
     1801 tcgcgaagac gttcggaaga tgcagacctg gctcgcgcaa ggggatcggc tcgtcggact
     1861 gcgtgaacac ctccttcggc aaaaggcgat ggcgacgctg gtcgcgtgcg tgtcagggga
     1921 tgtggcggcc ggcgaggctc ccccggcatc ggccccaggt cgtacggccg aagctccggg
     1981 ggcacctgta gggtaatggc gcgagggaag ggagctcgac cggtggagga ccaccccaag
     2041 ctctacatgc cgattgtcgt cgagcagacc gcgcgcgggg agcgggcgta tgacatctac
     2101 tctcgcctcc tccgcgagcg gctcatcttc atcggggggc cgatcgaaga tgagatggcc
     2161 aacctcatca tcgcgcagat gctctatctc gagtacgagg acccggagaa ggagatccag
     2221 ttgtatgtca acaccccggg cgggtcggcc acggcggggc tcgcgatcta cgacaccatg
     2281 cagtacatgc gcgcaccggt gtccacgatc tgcgtcgggc tggcggcgag cgcgggtgcg
     2341 atcatcctgg gcgggggcac caagggccgc cggttcgcgc tctcgtactc gcgcatgatg
     2401 atccaccagc cgtggggtgg agcccagggg accgtgtcgg acatcagcat ccagacgaag
     2461 gagttcctgg cgatccggca aatcctcaac gaagtgctgg cgaagcacac cgggcagccg
     2521 ctcgaacgcg tggagcggga taccgagcgc aacttctgga tgagcgccga agaagcgagg
     2581 gactacggcc tcatcgacga cgtgatcaag tcccgaaaac tggccgcgcc tccggccgcg
     2641 agccgatgaa cgatgtttaa attcggggac gagcgcgacc gcctgaagtg ctcgttttgc
     2701 gggaagacgc aggagcaggt gcgcaagctg gtcgccggcc cgggcgttta catctgcgac
     2761 gagtgcatcg agctctgcaa tgagatcatc gaagaagagc tctacgggga gccccacgga
     2821 cagaccaaat cgaaggggac cccgaagccc caggagatct accagacgct gtgtcagtac
     2881 gtggtcggcc aggaccgggc aaagaaggcc ctggccgtgg cggtctacaa ccactacaaa
     2941 cggatcgggt cgccccccaa gcgcgccacc gatgtggagc tgcagaagag caacatcctg
     3001 ctgatcgggc ccacggggtg cggcaagacc ctgcttgccc aaacgctcgc ccgggtcctc
     3061 gacgtcccgt ttgccatcgc cgacgcgacc tcgctgaccg aggcggggta tgtgggggag
     3121 gacgttgaga acatcctcct ccgtctcatc cagacgtccg actacgacgt caagcgcgcc
     3181 gaacgcggga tcgtctatat cgacgaggtc gacaagatcg cccggaagtc cgagaacccg
     3241 tcgatcaccc gagacgtctc gggagaaggc gtgcagcagg ccttgctcaa gatcctcgag
     3301 gggaccaccg ccaacgtccc tccccagggg ggacgcaagc atccgcacca ggagttcatc
     3361 cagatcgaca ccaccaacat tctgttcatc tgtggcgggg cgtttgacgg gctcgagcgg
     3421 atcatcgagt cccgcacccg ggtcgccagc atcggattcg gggcgaagat cgagcagaag
     3481 cgggagcagc ggatgagcga actgctgggg caggtcatgc cccaggatct gttgcggtac
     3541 gggctcatcc ccgagttcat cggccgcctc ccgatcgtcg taccgatcga gccgctggcc
     3601 gagtccgacc tggtcacgat cctggtggag ccccgcaacg ccctcgtccg gcagtaccag
     3661 aagattctgg agatggatgg ggtggagctc gtcgtgaccg acggcgcgct ccgcgcgatt
     3721 gcccgggaag cgatgagcct ccacaccggc gcgcgggggc tcaggaccgt gctcgaggag
     3781 atcatgctca acatcatgta cgagatccca tcccgcgggg acgtcaagcg gtgcgtcctc
     3841 gccgaagaga ccgtttccaa gcggatcgag ccgctgctgt tgaccgcgag cgacctcaag
     3901 aaggtgccca aggacaagcc cgcctgagtt ccccggcacc cccacccacg ccccaccgcc
     3961 tgcgcggacc ctccgaggga atcatttagg accggtttac cgtatatctg tgtatagtag
     4021 aaagggggga tggatcgacg cagccgacgc agtggttcac cgccccgagg acgggagggg
     4081 gaaagatgac ggagcagaag ccggaacaca aaccggacca caagcccgac caggagctgc
     4141 agcccacgct tccggagatc ctacccgtgc tgccgctcaa ggggacagtt gtgctcccgc
     4201 tgatggtggt ccccct
//
LOCUS       DASQGH010000227         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40620, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000227 DASQGH010000000
VERSION     DASQGH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40620"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..749)
                     /locus_tag="VEW91_04360"
     CDS             complement(336..749)
                     /locus_tag="VEW91_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM90850.1"
                     /translation="MSAHERTCLVIAPGKTFHGKQDLDYFAGISAQSAGARSLCMHLV
                     TIPPKVRATPHYHADHETAIYVLSGEAGMWYGEGLRQHLTVRAGELLYIPANVPHLPY
                     NPSETQTCTAVLARTDPNEQESVVPYAVPDPALPR"
     gene            complement(827..>2372)
                     /locus_tag="VEW91_04365"
     CDS             complement(827..>2372)
                     /locus_tag="VEW91_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HYM90851.1"
                     /translation="CHWHHARFDLASGGTFDQFAGDVRTFPVQVRQGEVWVDLAPHAD
                     PRAHHRARLGDGLERNIPLVLGKAVIALLESGEDPAVPFRVGLDFGTRYRLEGWGQGL
                     TILTSMMNLVPALDPDDRARALYHGLSAVARDCDGQPPRFGLRPLPMLAADAATLGRW
                     FRRFVEVRDAEGAERCIVSAVRAGADHRQLAGMLFAAATDHRYIQTGHVLDFTNKAFE
                     ALDAAGWEHAESVLTSLVPGYAAADRMEESNEWRHPVDLVGILERAFDALPEALREGR
                     DRHGPWGGQEELVPVLLGEDPQGIADALLDALRGGCPEAALATTVSYAAALRIAHFPT
                     TNEFGDWDTALHTFTFANAVHQGLRRAPSVELLRGVFDAAMSVHLDRFLNVPPAHLPE
                     ARASEDPPAILGGLPALLDRQQQVNEAAAHVARYLHSGGDPARLLAALGGLLLREDRD
                     FHTIQTIEGAFRLFSHLRRTPAAPNVLVAAARYLAAHAPTVRAQGQTFQIAHRLHRGE
                     RLFEAS"
BASE COUNT          347 a          845 c          838 g          342 t
ORIGIN      
        1 gcccgcgcgg gcgcgagccc ggctccggcg agcgccgagg cgatcgcgct cgacgacaac
       61 cccagcatcg ccaggcgggc gatcacctca cgacgcgaga gccggcccgt cgtccccagc
      121 gtgacgacct ggtgcacctc atcctttcgc atggactacc cccctgtgag cggtcttctg
      181 caagcgggtt ctggacttgg gcaggcagat tcgcgacgcc ccagcgaact ccctccgggt
      241 gccttgcccc acccgccctc ccgccttcca ctatcctcat gtacgcgggt gagcgcgggg
      301 gtgttcggcg ccaggagggg cgggctgccg cgaggctacc tgggaagcgc cggatcggga
      361 acggcgtacg ggacgacgct ctcctgctcg ttgggatccg tccgggccag gaccgcggtg
      421 cacgtctgcg tctcgctcgg attgtagggg aggtggggga cgttcgccgg aatgtacagt
      481 aactctcccg cccgcacggt caggtgctgg cgcagcccct caccgtacca catgccggcc
      541 tccccggaga gcacgtagat cgccgtctca tggtccgcat ggtagtgagg ggtggcccga
      601 accttggggg ggatggtgac caggtgcatg cacaggctcc tggctccggc gctctgcgcg
      661 gagatccccg cgaagtagtc gaggtcctgc ttcccgtgga acgtcttgcc gggagcgatc
      721 actagacacg tccgctcatg cgccgacatc gcgcctcccc ccctgcctgg gtattccggg
      781 ctagttacgc cgccccttcg gcgctgccct ttctctggcc cctgtctcag gaggcctcaa
      841 agagccgctc tccccggtga agccggtggg cgatctggaa cgtctgccct tgagcccgca
      901 cggtgggggc gtgcgcggcc aggtaccgcg cggcggccac cagaacgttc ggagccgccg
      961 gggtccggcg gaggtgcgag aacagccgga aggcgccctc gatcgtctgg atcgtgtgga
     1021 agtcgcggtc ctcccggagg agcaggccgc cgagtgcggc gaggagccgg gcgggatcgc
     1081 ctccgctgtg gagatagcgg gccacgtggg ccgccgcctc gttcacctgc tgctggcggt
     1141 ccaggagcgc cgggagcccc ccgaggatgg cggggggatc ctccgaggcc cgggcctcgg
     1201 ggagatgggc cggcgggacg ttcaagaagc ggtccaggtg gacgctcatc gccgcatcga
     1261 agaccccacg gagcaactcc accgagggag cccggcgaag cccctggtga acggcgttgg
     1321 caaaggtaaa ggtgtggagc gccgtatccc agtcgccgaa ctcgttggtg gtggggaagt
     1381 gcgcgatgcg gagcgcggcc gcgtaggaca ccgtcgtcgc gagcgcggcc tcggggcagc
     1441 ccccccggag cgcgtcgagc agcgcatccg cgatcccctg cggatcctcg cccagcagca
     1501 ccggcacgag ctcctcttgc ccgccccagg gaccgtgccg gtcccggccc tcccgcagcg
     1561 cctccggcag ggcatcaaag gcgcgctcca ggattcccac gagatccacc ggatgccgcc
     1621 attcgtttga ctcctccatc cggtcggcag ccgcgtaccc gggaacgagg ctggtcagca
     1681 cggactcggc gtgctcccac ccggccgcgt ccagggcttc aaaggctttg ttcgtaaagt
     1741 cgagcacgtg gccggtctgg atgtagcggt ggtcggtcgc ggcagcgaac agcatcccgg
     1801 ctagctgccg gtggtcggcc cccgcccgca ccgccgagac gatgcagcgc tcggctccct
     1861 cggcgtcccg gacctccacg aagcggcgaa accagcgccc cagcgtggcc gcatcggcgg
     1921 caagcatcgg gagcgggcga agcccaaacc gcgggggctg gccgtcgcag tcgcgggcca
     1981 ccgccgagag cccgtggtac agcgcccgcg cacgatcgtc cgggtccagc gcgggcacca
     2041 ggttcatcat gctggtgagg atcgtcagcc cctgccccca cccctcgagc cggtagcggg
     2101 tcccgaagtc cagcccaacg cggaacggca ccgcggggtc ctcgccactt tcgagcagcg
     2161 cgatcacggc cttgccgagg accagtggaa tgttgcgctc cagaccgtcg cccagtctcg
     2221 cccggtggtg ggcgcgggga tcggcgtggg gcgccagatc gacccagacc tcgccctggc
     2281 gcacctggac cgggaacgtc cggacatcgc cggcgaactg gtcgaacgtc ccgccgctcg
     2341 cgaggtcgaa cctggcgtgg tgccagtggc ag
//
LOCUS       DASQGH010000228         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12425, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000228 DASQGH010000000
VERSION     DASQGH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12425"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VEW91_04370"
     CDS             <1..440
                     /locus_tag="VEW91_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYM90852.1"
                     /translation="EQMMSDRKTDDIVDILAGLALFADLTRPQLQAVAHTFEEEWFTE
                     GQRILRQGFTGSNFYVILEGEAVIRINGETRATLGRGEFFGEVSVLLGEPPTADVVAL
                     RPLRCIALAGTEAEAFLTSYPRVMFRMLQAQARRLRSANLWRS"
     gene            428..1918
                     /locus_tag="VEW91_04375"
     CDS             428..1918
                     /locus_tag="VEW91_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYM90853.1"
                     /translation="MAQLTAQPFPPGTYPVVVVGSGPGGLQLSYFLQRLGVRHATVSE
                     DPGPGGMFRRYPLFQRLNTWSKPYAMADRGTRPYEWYDWNSLVADAPELRSLVSQSME
                     GVSYFPSRGEMERGLVAFAERAGVQVRYGCRWTGTRREGEEFVIVTSGGEYRCQMAVF
                     AVGMAVPWKPPTPGMDDVPHYVETKPARAYAGKRVFLIGKRNSGFEVADALLPWARQI
                     ILGSPRPPMLSVLASGGGVRAKYLVPYEDHVIGGGVFVLEAAIERVARLAEGWCVTTA
                     AVTGGVREFIVDEVIAATGFTTPLGDLPDLGVATFSRGRLPRMNPFWESTTVPGIYFA
                     GTITQGAFGLRKAGGGTGNSAAVGGFRHNAKVLAEHFAKKLGRTIERPTLGADAVVPY
                     LLSEATRAPELLNQKAYLARVVSLQQSRGITDEGILPLASFVDQSGPDAVAISVETDE
                     HGRHHPVAYVRRERRVSEHALPPNALLNFEGAEHQELLSGLLSGLV"
     gene            1996..>2408
                     /locus_tag="VEW91_04380"
     CDS             1996..>2408
                     /locus_tag="VEW91_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HYM90854.1"
                     /translation="MPTIAEKRHAFRQLHESGCFVIPNPWDAGTARYLQHLGFKALAT
                     TSSGAAFSMALPDADWALTRDPMLAHIRTIVEASDVPVNADFEGGFADDPDGVARNVR
                     LCVETGVAGLSIEDSTSDPAVPLYDFDLALARVRAA"
BASE COUNT          370 a          773 c          848 g          417 t
ORIGIN      
        1 gggagcagat gatgagcgac aggaagactg acgacatcgt cgacatcctt gcagggctgg
       61 cgctgttcgc cgacctgacc cgaccgcaac tgcaggcggt ggctcacacc ttcgaggaag
      121 aatggttcac ggagggacag cgcatcctcc gtcaggggtt caccgggtcg aacttctacg
      181 tgatcttgga aggcgaggcg gtgattcgca tcaacggcga gacccgcgcc acgttggggc
      241 ggggggagtt cttcggcgag gtctcggtgc tgctcggcga accgccgacc gcagacgtcg
      301 tcgcgctccg gccgctgcgg tgcatcgccc tggcgggtac tgaggccgag gcgtttctca
      361 catcctatcc tcgcgtcatg ttcaggatgc tccaggcgca agctcggcgg ctccggagcg
      421 caaacctatg gcgcagttga ctgcgcaacc gtttccgccc ggcacgtatc cggtcgtggt
      481 ggtcggcagc gggccgggag gccttcagct ctcctacttc ctccagcgcc ttggtgtgcg
      541 gcacgccacg gtctcggagg atcctggccc cggcgggatg ttccgccggt atccgctctt
      601 tcagcgcctt aacacatggt cgaagccgta cgcgatggcg gaccggggca cgcgcccgta
      661 tgaatggtac gactggaaca gtctggttgc cgacgcgccg gagctccggt ccctcgtctc
      721 gcagtcgatg gagggggtgt cgtacttccc gtcgcggggc gagatggagc gggggctcgt
      781 ggccttcgcc gagcgggccg gggtccaggt ccgctacggc tgccgttgga ctgggacccg
      841 gcgcgagggt gaggagttcg tgatcgtcac gtccggtggt gagtaccggt gccagatggc
      901 cgtgttcgcg gtgggcatgg ccgtcccgtg gaagccccca acccccggga tggatgatgt
      961 cccccactat gtcgagacca agcccgctag ggcgtatgcg ggcaagcggg tctttctgat
     1021 cggcaagcgc aattcagggt tcgaggtcgc cgacgcgctc ctcccctggg ccaggcagat
     1081 catcctggga tctccacgcc cgcccatgct ctccgtgctg gcctccggcg gaggcgtccg
     1141 cgcgaagtac ctggtgccct atgaggatca cgtcattgga ggcggcgtgt ttgtcttgga
     1201 ggcggcgatc gaaagggtgg cgcggctggc cgaggggtgg tgcgtcacca ccgcagcggt
     1261 gactggcggg gtgcgagagt ttatcgtgga cgaggtgatc gccgccaccg ggttcacgac
     1321 gccgctcggg gacctccccg atctcggcgt ggcaaccttc agccggggcc gcctgccgcg
     1381 gatgaatccc ttctgggaaa gcaccacggt gcccgggatc tacttcgcag ggacgatcac
     1441 ccagggggcg ttcggactca ggaaggccgg cggcggcacg ggcaactcgg ccgcggtcgg
     1501 gggcttccgc cacaacgcca aggttctggc ggagcatttc gccaagaagc tcggccgtac
     1561 gatcgagagg ccaaccctgg gagccgacgc ggtggtcccg tacctgctct ccgaggccac
     1621 ccgcgcgccc gaactgttga atcaaaaggc gtacctcgcc agggtggtca gtctccagca
     1681 gagccgtggg atcacagatg aggggatatt gccgctcgcc tccttcgtcg accagtccgg
     1741 accggatgcg gtggcgatct cagtggagac cgacgagcac gggcgccacc atcccgtggc
     1801 ctacgtccgg cgggagaggc gggtgagcga gcacgcgctt ccgccaaacg cgcttctcaa
     1861 cttcgagggc gccgagcacc aggaactgct ctcgggtctg ctgtcggggc tcgtttagcc
     1921 cggatctcac ggcggtccgg gacgtgtccc gaccggtgac gccggacttg agggggaaga
     1981 gcatgggaga gcacgatgcc cacgattgct gagaagcgcc acgcctttcg tcagctccac
     2041 gaatccggct gcttcgtcat ccccaatccg tgggacgccg gcacggcgcg ttacctgcag
     2101 catctcgggt tcaaggcgct cgcgaccacg agctccggtg ccgccttctc gatggcgctg
     2161 ccggacgcgg actgggcgct gacccgcgat ccgatgctcg cgcacatccg caccatcgtc
     2221 gaagcgtccg acgtgccggt gaacgccgat ttcgagggcg gctttgccga cgacccggac
     2281 ggtgttgccc ggaacgtgcg gctgtgcgtg gaaaccggcg tggcgggctt gtcgatcgag
     2341 gattcgacca gcgatccggc ggtcccgctg tacgacttcg atctcgcgct cgcacgtgtg
     2401 cgcgccgc
//
LOCUS       DASQGH010000229         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12431, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000229 DASQGH010000000
VERSION     DASQGH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12431"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VEW91_04385"
     CDS             complement(<1..335)
                     /locus_tag="VEW91_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HYM90855.1"
                     /translation="MTERAQIDEAAAAVRRRMPAAPEVGIILGSGLGALADAAEVDAA
                     VAYTEIPHFPVSTASGHRGRLVLGRLEGKRVAILQGRVHLYEGYTAAQVAFPVRVLSA
                     LGIRMLAVT"
     gene            complement(332..1507)
                     /locus_tag="VEW91_04390"
     CDS             complement(332..1507)
                     /locus_tag="VEW91_04390"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HYM90856.1"
                     /translation="MRPQRVLVIVLDSCGVGALPDARYSDEGTSTLPHTAAACGGLHL
                     PTLGRLGLGRLAPIPGVPPDPAPAGAFGKMAERSSGKDSTTGHWELMGIVLDRPFPTY
                     PGGFPREVIEPFEARIGRRVLGNRAASGTEIIQELGEEHLRMGAPIVYTSADSVFQIA
                     AHESCIPVDELYRYCRIARQILTGPHAVGRVIARPFVGEPGHFVRTDRRRDFSLPPPA
                     PTVLDRIHRAGLPVVAIGKISDLFCGQGITQTVHTHNDLDGMRATRQALETTPAGLIF
                     TNLVDLDMLFGHRNDPHGYGQDLEQIDAELAQVTRSLSPKDLLMVTADHGNDPTTPGT
                     DHSREYVPVLVAGPSVRAGVDLGIRSTFADLGATVAQALGAAAPEVGTSFLDEVLAG"
BASE COUNT          223 a          558 c          586 g          224 t
ORIGIN      
        1 ttcgtgacgg cgagcattcg gatgccgagg gcgctcaaga ccctgacggg aaacgccacc
       61 tgcgcggcgg tgtagccctc gtacagatgc acgcgcccct gcaggatggc gacccgcttc
      121 ccctcgaggc gcccgagcac cagccggccc cggtggcccg atgcggtcga caccgggaaa
      181 tgcgggatct ccgtgtacgc caccgcggcg tccacctcgg cggcatcggc cagggcgccg
      241 aggccgctcc cgaggatgat cccgacctcg ggagccgcgg gcatccggcg ccggaccgcc
      301 gcggcggctt cgtcgatctg ggcgcgctcc gtcatccggc gagcacctcg tcgaggaagc
      361 tcgtgcccac ctccggcgcc gcggccccca gcgcctgggc cacggtagcg ccgaggtccg
      421 caaacgtcga tcggatcccg aggtcgaccc cggcccgcac cgagggcccg gcgaccagga
      481 ccggaacgta ctcccggctg tggtcggtcc cgggggtcgt gggatcgttg ccgtgatcgg
      541 cggtgaccat caggagatcc tttgggctga gggacctggt cacctgcgcc agctccgcgt
      601 cgatctgctc gaggtcctgc ccgtacccgt gggggtcgtt ccggtgccca aacagcatgt
      661 cgaggtccac gaggttcgtg aagatgagcc cggccggcgt ggtctccagg gcctggcgcg
      721 tcgcccgcat cccgtcgagg tcgttatgcg tgtggaccgt ctgggtgatc ccctgcccgc
      781 aaaacagatc ggagatcttc ccgatcgcga cgaccgggag cccggcgcgg tggatccgat
      841 cgagcaccgt gggggcgggc ggcggcaggg agaaatcgcg ccgccggtcg gtgcgcacga
      901 aatggccggg ctcgcccaca aacggccggg cgatcacccg gcccacggcg tgggggccgg
      961 tgaggatctg gcgcgcgatc cggcagtacc ggtacaactc atcgaccgga atgcacgatt
     1021 cgtgcgcggc gatctggaac acgctgtccg cggacgtgta cacgatgggc gcgcccatgc
     1081 gcaggtgctc ctcccccagt tcctggatga tctccgttcc ggacgcggcg cggttgccga
     1141 ggacgcggcg gccgatccgc gcttcgaacg gctcgatcac ctcacgggga aaccccccgg
     1201 ggtaggtggg aaacggccgg tcgagcacga tgcccatgag ctcccagtgc ccggtcgtgc
     1261 tgtccttccc ggatgagcgc tcggccatct tcccaaacgc gccggcgggc gcgggatccg
     1321 gcggcacgcc ggggatgggc gccaggcgcc cgagccccag gcgccccagg gtgggcaggt
     1381 ggagtccccc gcaggcggcg gcggtgtgcg ggagcgtgct ggtcccctca tcgctgtacc
     1441 gggcgtcggg cagcgcgccg actccgcagc tgtcgaggac gatcacgagg acccgctgcg
     1501 ggcgcatcct gggggggagc gtcgccggag cgcgtcagcc ggtgtggccg aggtgctccc
     1561 gcgcgactgt gatgccgatg atcgacttgg c
//
LOCUS       DASQGH010000230         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_51908, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000230 DASQGH010000000
VERSION     DASQGH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51908"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      196..344
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            398..>1600
                     /gene="ribD"
                     /locus_tag="VEW91_04395"
     CDS             398..>1600
                     /gene="ribD"
                     /locus_tag="VEW91_04395"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYM90857.1"
                     /translation="MFLLRTDSDLDERYMREALDLAGLGAGTASPNPMVGAVVVSGSR
                     VVGRGYHAQPGLPHAEVLALDEAGAAAGGATLYVTLEPCAHWGRTPPCTDAIIRAGIR
                     RVVAAMEDPDRQVRGRGLRRLAEAGIETSLGTAEDAARRLNEAYVKHRTTGLPFVTAK
                     WAMSVDGRIATRSGDSRWISGAASRSVAHALRAASDAILVGIGTVLRDDPQLTVRDLP
                     GEHRGGRQPARIVVDSRLRIPLGARVLARDGAGVIVATTRHAHPDGRRSLEALGVEVV
                     VADGPDGRVDLRVLCEVLGRRGVTSLLVEGGGAVHGACIDAGLVDKVVVFVAPLILGG
                     PAPGPVGGAGVERLVEAWRLTQTTMRQVEQDIVIEGYLTAPPRAEPARGIAEGGARGG
                     ARTPCSPAS"
BASE COUNT          190 a          518 c          643 g          249 t
ORIGIN      
        1 ggttgcgggg cggggtggcg atcgcgctcg tgttggacct cgcccccggc ctgccggaac
       61 gagacgcggt ggccgcggcg gtctttggcc tcgtcgtcgt tacgttgctc gggcagggcc
      121 tctcgatgcc ggcgctgatg cggcgcgtcg gggtgctgcg cgattgaccc gccccggctc
      181 cgtttgctat catcggtgtg ccttcggggc agggtgagcc ggtagcgagt cgccggcaat
      241 tcccgatcgg cggtagagcc cgcgagcccg ttccccttgt gggactgggc atgatctggt
      301 gagagtccag agccgacggt agagtccgga tggagaaggc gcggcccaag catcatttgg
      361 gcggggatgg gttgccccgg gggcatctgc tcgcggggtg tttttattgc ggaccgattc
      421 ggatctggat gagcggtaca tgcgcgaagc gctggacctg gcgggcctcg gcgcggggac
      481 ggcgagcccc aacccgatgg tcggcgccgt cgtcgtctct gggagccggg tcgtggggcg
      541 ggggtatcac gcgcagccgg ggttgcccca cgccgaagtc ctcgcgctcg atgaagccgg
      601 ggcggccgcc gggggcgcga cgctctacgt gaccctcgag ccctgcgccc actggggacg
      661 gacgccgccg tgcacggatg cgatcatccg cgcgggcatc cgccgcgtcg tcgccgcgat
      721 ggaagatccc gaccgccagg tgcgcggccg ggggctgcgc cgcctcgccg aggcgggcat
      781 cgagacgagc ctgggaacgg cggaggacgc cgcgcgccgc ctcaacgagg cctacgtgaa
      841 gcaccgtacc acggggctgc cgttcgtgac ggcgaagtgg gcgatgagcg tcgacggccg
      901 catcgccacg cggtcgggcg actcccggtg gatttcggga gcggcgtccc gctcggtcgc
      961 gcatgcactg cgcgccgcct cggatgcgat cctggtcggc atcgggacgg tcttgcgtga
     1021 cgatccacag ttgaccgtgc gcgacctccc aggggagcat cggggcggtc ggcagccggc
     1081 ccgcatcgtg gtggatagcc gtctccggat tccgctcggc gcccgcgtgc tcgcccgcga
     1141 tggtgccggg gtgatcgtgg ctacgacccg ccacgcgcac ccggatggcc gccgctccct
     1201 cgaggcgctc ggcgttgagg tcgttgtcgc ggacgggccg gacggccggg tcgatctcag
     1261 ggtgctgtgc gaggtgctcg gccggcgggg cgtcaccagc ctgctggtcg agggcggagg
     1321 cgcggtccac ggcgcctgca tcgacgccgg gcttgtcgac aaggtcgtgg tgtttgttgc
     1381 gccgctgatc ctgggcggac cggctcccgg gcccgtaggt ggcgccggcg tggagaggct
     1441 ggtggaggcc tggcgcctca cccagacgac gatgcggcag gtggagcagg atatcgtgat
     1501 cgagggctac ctcaccgctc cgccgcgggc ggagcccgcc cgcggaatcg cggagggtgg
     1561 ggcgcggggg ggcgcgagga caccgtgttc accggcatcg
//
LOCUS       DASQGH010000231         9676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35000, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000231 DASQGH010000000
VERSION     DASQGH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35000"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEW91_04400"
     CDS             complement(<1..222)
                     /locus_tag="VEW91_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90858.1"
                     /translation="MKRYWVRVGQEEFEVGVEDPEGTLLVTVAGRSYRAELTETVPSW
                     YTLVVDGACHDLAIRGRPAPWVLPLDGCSY"
     gene            complement(219..1709)
                     /locus_tag="VEW91_04405"
     CDS             complement(219..1709)
                     /locus_tag="VEW91_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006172063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYM90859.1"
                     /translation="MSPAPRRLRKVLVANRGEVALRVIRACRARGLATVAIYSDADRG
                     ALHVRAAEEAYRVGGPEPAESYLNIPAIVDAARRAGADAVHPGYGFLSENPALAHACT
                     QARLVFVGPPADTLALCGDKAATRRRAAAAGVPLLAGTEPVDDHAAIAASERLGFPVL
                     IKAAAGGGGKGIHLVARPAALPGALRLARGEARSAFGDDRVYLEQWLEGPRHIEVQIL
                     ADDHGSVIHLGERDCSIQRRHQKLIEESPAPRLPDGLREALRHAAVTAARAVAYRNAG
                     TVEFLVSRGSFYFLEINARLQVEHPVTEMVTGIDLVARQLGVAAGEPLPGGQDQIRLR
                     GHAIECRISAEDAENQFLPWVGRIGAVLLPGGPWVRIDGALAPGMEISRHYDPLLAKI
                     IAWGSVREEAIARMAAALGETVVTGVPTTVPFHRWAMAHPAFRSGEYTTRFVEAEWAP
                     ARARPEGWLAALAAAALAYRDEQKTVILPPQGPSGWIRAARQEGIA"
     gene            complement(1719..3242)
                     /locus_tag="VEW91_04410"
     CDS             complement(1719..3242)
                     /locus_tag="VEW91_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYM90860.1"
                     /translation="MAARRARVEAGGGPARTARQHEAGKLTARERILELLDPGTFVEL
                     DRFVTHRATAFGMDKVDAPADGVVTGYGAVHSRLVYVFSQDFTVLGGSLGEGHAQKIC
                     KIMDLALRNGAPVIGLNDSGGARIQEGVMSLGGYAEIFLRNTLASGVVPQISAIMGPC
                     AGGAVYSPAITDFTVMVRGTSYMFVTGPQVVKAVTHEDVTFDELGGAEVHASRSGVAH
                     FIADSDPDALQLVRRLLGYLPQNNQDDPPRAASTDDPNRMDAALDAVVPGDPHRPYDM
                     HEVITRIVDDGEFLEVHRHFAQNLVVGFARLDGYPAGIVAQQPAVLAGTLDINSAVKG
                     ARFVRFCDAFNIPLVTFVDVPGFMPGTAQEHGGIIKHGAKLLYAYCEATVPKLAVITR
                     KAYGGAYDVMSSKHIRGDLNLAWPTAEIAVMGPEGAIDIIFRRELAEGQDREAARARL
                     VAEYRQQFANPYIAAGRGYLDDIIEPRETRPRLTSALRMLRGKRDRNPPKKHGNIPL"
     gene            complement(3294..4433)
                     /locus_tag="VEW91_04415"
     CDS             complement(3294..4433)
                     /locus_tag="VEW91_04415"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HYM90861.1"
                     /translation="MPKQILAEEIERFLGTVDGVTSARLLTSPSGAIDQIYVTASDVT
                     ERRAARRAIVAALMTTYGIPVEPWRVQVAHLRRFPIAELRDLKVVRVEETLSATETVA
                     RVQVTWARAGEPRMGTGQAAALAGAGHRLRALAEATIDAVRAVVEPAYRRIAVQQVSV
                     ASSMDRPVVLVGITAGSPLGQETFVGAAFQREGTPDAAVTATLDAVIKWLLHAAADES
                     GEGAEGDRRARLEAMRHFVRADGGGSPVPQVPPAPASPAASVPGPALLPPAWSPRPPS
                     SPDVLEDLHEIRPEQKGGAVMATHHEGARAGMASPRPARPAVEEEFYQPLMEARTPVH
                     IRCRDGYEIAHAILRDVGTYTILVEVGGATELVYKHAIISIRPRT"
     gene            complement(4518..5015)
                     /locus_tag="VEW91_04420"
     CDS             complement(4518..5015)
                     /locus_tag="VEW91_04420"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HYM90862.1"
                     /translation="MFVLLAAGLLVWWLIHPRAAGVEVYFVRFDAVRHLGSLVAVRRP
                     VPRGPIEGRLRAALHALLAGPRVGGEGAARDLTTEIPPGTTLLGVQINGRIVTVNLSK
                     TYAAGGGSTSMLARVWQVVYTATQFPEAPEVQILVDGRRVEGLGGEGVMIGAPLRRPL
                     VPPSF"
     gene            complement(5060..7099)
                     /gene="ligA"
                     /locus_tag="VEW91_04425"
     CDS             complement(5060..7099)
                     /gene="ligA"
                     /locus_tag="VEW91_04425"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYM90863.1"
                     /translation="MSTERRRPVQRRPAADVARSIEDLREQIRHHLYLYHVLNAPEIS
                     DEAFDALMDELRALEARHPDLVTPDSPTQRVGAPPAEGFRPITHPQPMLSLANAFDEE
                     DLRAWHKRVRAGLGDRAVEFVCELKFDGAAISLVYEHGVFTQGATRGDGVRGEDITPN
                     LRTVRALPLRLRREADPPAFVEVRGEVYLPTRALEEINQERVQAGQTPFANTRNAAAG
                     SLRQLDPAVTARRPLDLYVYQLGAVRGRRFTTHWESLEWARTAGLPVNAHARRVASID
                     DVLAYVRDWSGRRGTLPYGTDGVVVKVDSLDQQAELGATSQAPRWAIAYKFPAEQAET
                     RVLDIAVQVGRTGALTPVAALEPVRVSGVIVRRATLHNEDEMRRKDVRIGDHVIVQRA
                     GEVIPEVVRVLPEKRTGAERRFEMPAACPVCGSPVERRAGEAVSRCTGGASCPAQVLE
                     RLIHFGSRDALDIEGVGPKLLQQMLDRDLIRDPADLFTLTKAQVLTLERMADKSADNV
                     IAAIARSRRPSPSRLIYALGIRHVGVHVAELLGQPFPDLARLADASFEEVRDVPGIGP
                     TIAQSVVEFFNQPSTRALLHKLRAAGVRPQAPAASRGRLAGKTFVFTGTLERLSRRDA
                     ADRVTALGGAVSDGVSARTTYLVAGAASGSKLDRARKLGVAVLDEPAFLKLVEGR"
     gene            complement(7149..7529)
                     /locus_tag="VEW91_04430"
     CDS             complement(7149..7529)
                     /locus_tag="VEW91_04430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYM90864.1"
                     /translation="MNRQSVFPTGRAAPLAPYSPGIRFGNLLFTAGAVGMDAKGKVPP
                     DFREQTRNTMENLRAVLEAAGSGFDHVLKVTVFLTDMKDFAAMNEVYRSYFTGDLPAR
                     STIGVAALARPELTVEIEMVAHIP"
     gene            complement(7601..8518)
                     /locus_tag="VEW91_04435"
     CDS             complement(7601..8518)
                     /locus_tag="VEW91_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYM90865.1"
                     /translation="METETLDVVTGAFGFTGGAITRRLLSKGRRVKTLTRRPDRPNPF
                     GERVRVAAYRFDDPDELARSLEGAAVLYNTYWVRFPYGRATFAEAIANTRTLLRACEA
                     ARIRRIVHLSVTSASEDSPLPYFRGKGILERAIRESALSYAILRPGLIYGPGDILLNN
                     IAWFLRHFPAFVIPDDGGYRLQCADVEDVAEVAVSAAERAENFVVDVVGPETFTFDEL
                     VRLIAGAIRSRARIVHLPPGLVRILLGMAGRLVHDVILTPDEITGLMSDLLVSRGAPV
                     GRRRLRDWLDEHGPRLGRTYASELGRHYP"
     gene            complement(8581..8865)
                     /locus_tag="VEW91_04440"
     CDS             complement(8581..8865)
                     /locus_tag="VEW91_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYM90866.1"
                     /translation="MSVSVRIPTPLRRITNGERVVTVDGSDLAQALADLDRRFPGIRA
                     KILDDRGEVLQFVNIFVDEQDIRFLSGLRTPLKDGAEVSIVPAMAGGAPA"
     gene            complement(8887..>9676)
                     /gene="thrC"
                     /locus_tag="VEW91_04445"
     CDS             complement(8887..>9676)
                     /gene="thrC"
                     /locus_tag="VEW91_04445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYM90867.1"
                     /translation="RAVVFIPKGLERAKVITTSVYRPTVVEVDGTYDDVNRLCLEIGD
                     EHRWAIANVNLRPYYSEGAKTLAFEVAEQLGWRAPDRVIVPVASGNMFVKIHKGFEEF
                     ERLGVIPRQSVRMTAAQAEGCGPIATAYKQKTDKVLPVRPNTIAKSLAIGAPADGAYV
                     LDLARRTGGAVESVTDEETVDAIRLLAQTEGLFTEPAGGVTVGVLRKLAASGVIGPDE
                     VTVAYITGIGLKALEAVEEVVEAAVSIKPTLASFEKHVLALAGG"
BASE COUNT         1380 a         3533 c         3398 g         1365 t
ORIGIN      
        1 ataactgcat ccatcgaggg gcagcaccca cggggcgggg cgaccccgga tggcgagatc
       61 gtggcacgcg ccgtcgacga cgagggtgta ccatgagggc accgtttcgg tcagctcggc
      121 ccggtacgag cggccggcca ccgtcacaag gagcgtgccc tccgggtcct ccaccccgac
      181 ctcgaactcc tcctgcccta cgcgcaccca gtagcgcttc acgcgatccc ctcttggcgc
      241 gcggcccgga tccaccccga cgggccctgg gggggcagga tcaccgtctt ctgctcgtcg
      301 cggtaggcga gggccgcggc ggcgagcgca gccagccagc cctcgggacg ggcccgcgcg
      361 ggagcccatt ccgcctccac gaatcgggtg gtgtactccc ccgagcgaaa cgccgggtgg
      421 gccatcgccc accggtggaa cggcaccgtg gtcgggaccc cggtcaccac cgtctccccg
      481 agcgcggccg ccatcctggc gatcgcctcc tcgcggaccg atccccaggc gatgatcttc
      541 gcgagcagcg gatcgtaatg gcggctgatc tccatgccgg gggccagcgc tccatcgatc
      601 cggacccagg ggcccccggg gagcaggacc gcgccaattc gaccgaccca cggaaggaac
      661 tggttctcgg catcctccgc cgagatccgg cattcgatcg cgtgcccgcg caaccggatc
      721 tggtcctggc cacccgggag cggctcgccg gcggccaccc ccagctgccg ggcgaccagg
      781 tcgatgccgg tcaccatctc ggtgaccggg tgctcgacct gcaggcgggc gttgatctcc
      841 aggaagtaga acgatccgcg cgagacgaga aactccacgg tgccggcgtt ccggtaggcg
      901 acggcgcggg ccgcggtgac ggccgcgtgc cggagcgcct cccggagccc gtccgggagg
      961 cgcggcgcgg gcgattcctc gatcagcttc tggtgccgcc gctggatgga gcagtcacgc
     1021 tcgcccaggt ggatcaccga tccgtgatcg tccgccagga tctgcacctc gatgtgccgg
     1081 gggccctcga gccactgctc caggtacacc cggtcgtccc caaacgcgct ccgggcttcg
     1141 ccgcgcgcca accgcagcgc ccccggcagg gccgcgggcc gcgccaccag gtggatcccc
     1201 ttgcccccgc cgccggcggc cgccttgatg agcaccggaa accccaggcg ctcggaggcg
     1261 gcgatcgccg cgtggtcatc gacgggctcc gtccccgcca gcagcgggac gccggcggcg
     1321 gccgcgcggc gccgcgtcgc ggccttgtcc ccgcacaacg ccagcgtatc cgcggggggg
     1381 ccgacgaaga ccaggcgggc ctgggtgcac gcgtgcgcca gggcggggtt ctcggagagg
     1441 aatccgtacc cggggtgcac cgcatcggct cccgcgcggc gggccgcgtc cacgatcgcg
     1501 ggaatgttca ggtacgactc ggccggctcg ggccccccga cccggtaggc ctcctccgcg
     1561 gcgcggacat gcagggcccc ccggtcggca tcgctgtaga tcgccaccgt cgcgaggccc
     1621 cgcgcgcggc acgcccggat cacgcgcagg gcgacctctc cccggttcgc gacgagcacc
     1681 ttgcgcagac gccggggcgc cgggctcacg cgcgcgccct acaacgggat gtttccgtgc
     1741 ttcttcgggg ggttgcggtc ccgcttgccc cggagcatcc gcagcgccga ggtgagccgc
     1801 gggcgggtct cgcggggctc gatgatatcg tcgaggtacc ctcggccggc ggcgatgtat
     1861 ggattggcga actgttgccg gtactcggcc accagccggg cccgggccgc ctcccgatcc
     1921 tgtccctcgg ccagctcccg cctgaagatg atgtcgatcg ccccctccgg ccccatgacg
     1981 gcgatctcgg cggtgggcca cgccaggttc aggtcgccgc ggatgtgctt gctcgacatc
     2041 acgtcgtacg cgccgccgta cgctttccgg gtgatcaccg ccagcttggg gaccgtggcc
     2101 tcgcagtagg catagagcag cttggccccg tgcttgatga tcccgccgtg ctcctgcgcg
     2161 gtccccggca tgaacccggg gacgtccaca aaggtcacca gcgggatgtt gaacgcatcg
     2221 cagaaccgca cgaaccgggc gcccttgacg gcactgttga tatcgagcgt ccccgcgagc
     2281 accgcgggct gctgggcgac gatgcccgcc gggtacccgt ccagccgggc gaacccgacc
     2341 acgaggttct gggcgaagtg ccggtggacc tcgagaaact cgccgtcgtc gacgatgcgg
     2401 gtgatgacct cgtgcatgtc gtacggccgg tgaggatcgc cggggaccac cgcgtccagc
     2461 gcggcgtcca tccggttcgg atcgtcggtc gaggcggcgc gcggtgggtc gtcctggttg
     2521 ttctgcggaa ggtacccgag caggcgccgg acgagctgga gggcgtcggg atcgctgtcg
     2581 gcgatgaagt gcgccacccc gctccggctg gcgtggacct cggcgccgcc caactcgtcg
     2641 aacgtcacgt cctcgtgggt gaccgccttg accacctggg gcccggtgac gaacatgtag
     2701 ctcgtcccgc gcaccatcac cgtgaagtcc gtgatcgcgg gcgaatagac ggcccccccc
     2761 gcgcacggcc ccatgatggc cgaaatctgg gggacgaccc cgctggccag ggtgttgcgg
     2821 aggaagatct cggcgtaccc gccgaggctc atcacgcctt cctgaatccg cgcgcccccg
     2881 gaatcgttca gcccgatcac cggcgcgccg tttcggaggg caagatccat gatcttgcag
     2941 atcttctgcg cgtgcccctc ccccagcgaa ccgccgagga ccgtgaagtc ctgcgagaag
     3001 acatagacca atctcgagtg gaccgcccca tagccggtca ccacgccatc cgccggggcg
     3061 tcgaccttgt ccatgccgaa cgcggtggcc cggtgggtga cgaaccggtc cagttccacg
     3121 aacgtccccg ggtcgagcag ttcgaggatc cgctcgcggg cggtcagttt cccggcttcg
     3181 tgctggcggg cggtgcgggc gggaccgccc cctgcctcga cgcgggcgcg gcgggccgcc
     3241 agatcacgcc cccggctcct cgggccgtct ggatcggacg gggcgttcgg cacctaggtg
     3301 cgcggccgga tcgagatgat cgcgtgcttg tagaccagtt cggtcgcccc gccgacctcc
     3361 accaggatgg tgtaggtgcc gacatcccgg aggatggcat gcgcaatctc atagccgtcc
     3421 cggcagcgga tgtgcacggg ggttcgcgcc tccatcagcg gttgatagaa ctcctcctcg
     3481 accgccgggc gcgcggggcg cggcgacgcc atccccgcgc gcgcgccctc gtggtgtgtt
     3541 gccatcacgg cacctccctt ttgctcggga cggatctcgt ggaggtcctc gaggacatcg
     3601 gggctggacg gcgggcgcgg ggaccacgcg ggcgggagca gcgccggacc cggcacagag
     3661 gcggcggggg aggcgggagc gggcggtacc tgcgggacgg gagacccgcc tccgtccgct
     3721 cggacaaagt ggcgcatcgc ctcgaggcgc gcccggcggt cgccttcggc cccctccccg
     3781 gactcgtcgg ccgccgcgtg cagcagccac ttgatcaccg cgtcgagggt cgcggtcacc
     3841 gccgcgtcgg gcgtgccctc gcgctggaac gcggcgccca cgaacgtctc ttgaccgagc
     3901 ggggagccgg ccgtgatccc gacgagcacc accggccggt ccatggagct ggcgaccgag
     3961 acctgctgca ccgcgatccg ccggtaggcc ggctcgacga ccgcccgaac ggcgtcgatc
     4021 gtggcctcgg ccagcgcccg aaggcggtgc ccggcaccgg ccagggcggc ggcctgcccg
     4081 gtgcccatgc gcggctcgcc ggcgcgcgcc caggtcacct ggacccgtgc cacggtctcg
     4141 gtggcggaca gggtctcctc gacccgaacg accttgagat cccgcaactc ggcgatgggg
     4201 aaccgccgga ggtgggcgac ctggaccctc cacggctcga cggggatccc gtaggtggtc
     4261 atcagcgccg cgacgatcgc ccggcgcgcc gcccggcgtt ccgtgacgtc gctcgccgtc
     4321 acgtagatct ggtcgatcgc gccggagggg gacgtcagca gcctggccga cgtcacgccg
     4381 tccacggtcc cgagaaaccg ctcgatctcc tcggcaagga tctgcttggg catcggcggg
     4441 gatattcttc ccaccgggag atcgcccctc tatccgaacg gacgatcaca cccacccgtc
     4501 ccgcggaccg ccccgcctca gaacgacggg ggcacgagcg ggcgcctgag gggagcgccg
     4561 atcatgaccc cttcgcctcc cagcccctcg acgcgccggc cgtcgacgag gatctggacc
     4621 tccggggcct cggggaactg cgtcgcggtg tacacgacct gccagacccg cgccagcatg
     4681 ctcgtgctcc cgccgccggc ggcgtaggtc ttggagaggt tcaccgtcac gatcctgcca
     4741 tttatctgca cgccgagcag cgtggtgccg ggcgggatct cggtggtcag atcccgcgcc
     4801 gcgccctccc cgcccacccg gggcccggcg aggagggcgt ggagcgcggc ccgcagccga
     4861 ccctcgatcg gcccccgggg gaccgggcgg cgcacggcga cgagcgagcc caggtggcgg
     4921 acggcgtcga accggacgaa gtagacctcc accccggccg cccgcgggtg gatgagccac
     4981 cagacgagca gcccggcggc gagcagcacg aacacgaccg cgagcggtct cctggatctc
     5041 ctggatcggc gccgggccat caccggccct ccactagctt caggaacgcc ggctcgtcga
     5101 gcaccgcgac gcccagtttg cgcgcccggt cgagtttgct ccccgacgcc gcgccggcca
     5161 ccaggtacgt ggtgcgcgcg ctgacgccgt ccgagaccgc accacccagg gccgtcaccc
     5221 ggtcggcggc gtccctgcgc gacaggcgct cgagcgtgcc cgtgaacaca aacgtcttgc
     5281 ccgcgaggcg tccccgtgag gcggcgggcg cttgcgggcg caccccggcc gcgcgcagct
     5341 tgtggagcag cgcccgggtc gacggctggt tgaaaaactc caccacgctc tgcgcgatcg
     5401 tcgggccgat gccggggaca tcccggacct cctcgaacga ggcgtcagcg aggcgggcga
     5461 gatccgggaa gggctggccg aggagttcgg cgacgtgcac ccccacgtgc cggatcccga
     5521 gggcgtagat cagccgggag ggggacggcc ggcggcttcg ggcgatcgcg gcgatgacgt
     5581 tgtccgccga tttgtccgcc atgcgctcca gggtcaggac ctgggccttg gtcagcgtga
     5641 agagatccgc gggatcgcgg atcaggtcgc gatccagcat ctgctggagc agcttcggtc
     5701 ccacgccctc gatgtcgagc gcgtcccgcg atccaaaatg gatcaggcgc tccagcacct
     5761 gcgccgggca ggacgcgccg ccggtgcacc ggctcaccgc ttcgcccgcc cgccgctcca
     5821 ccggcgagcc gcacaccgga caggcggcgg gcatctcgaa ccgccgctcc gcgcccgtgc
     5881 gcttctccgg gagtacccgg acgacctcgg ggatcacctc gccggcccgc tggacgatca
     5941 catgatcccc gatccgcacg tccttgcgcc gcatctcgtc ctcgttgtgc agcgtggccc
     6001 gccgcacgat caccccggag acccgcaccg gctcgagcgc cgccaccggg gtgagcgcgc
     6061 cggtgcgccc gacctggacc gcgatgtcga ggacgcgcgt ctcggcctgc tcggccggga
     6121 acttgtacgc aatcgcccac cggggcgcct ggctcgtcgc gccgagctcc gcctgctggt
     6181 cgagcgaatc caccttgacc accacgccgt cggtgccgta gggtagggtc ccgcggcggc
     6241 ccgaccagtc gcgcacgtag gcgaggacat cgtcgatcga cgcgacgcgg cgcgcgtgcg
     6301 cgttcaccgg gaggccggcc gtgcgcgccc actcgaggct ctcccagtgg gtcgtgaatc
     6361 gcctcccgcg gacggccccc agctggtaga cgtagaggtc gagcggccgc cgggcggtca
     6421 ccgccggatc gagctggcgc agggagccgg cggcggcgtt ccgggtgttg gcgaacgggg
     6481 tctgcccggc ctgcacccgc tcctggttga tctcctccag cgcccgggtc gggaggtaca
     6541 cctcgccgcg gacctccacg aacgccggcg ggtccgcctc ccgccgcagc cggaggggga
     6601 gcgcgcgcac cgtgcggaga ttcggggtga tgtcttcccc ccggacgccg tccccccgcg
     6661 tcgcgccctg ggtgaagacc ccatgctcgt agacgagcga gatcgcggcg ccgtcaaact
     6721 tcagctcgca gacgaactcc accgcgcggt cccccaaccc ggcgcgcacc cgcttgtgcc
     6781 acgcgcggag atcctcctcg tcgaacgcat tggccaggct gagcatcggc tgggggtggg
     6841 tgatcggacg gaacccttct gccggcggcg cccccacccg ctgggtgggg gagtcggggg
     6901 tcaccaggtc gggatgccgg gcctcgagcg cccgcagctc gtccatcagg gcgtcgaagg
     6961 cctcgtccga gatctccggc gcattgagga cgtggtagag gtagaggtga tggcggatct
     7021 gctcgcggag gtcctcgatc gacctggcga catccgccgc cggccggcgc tgcaccgggc
     7081 ggcggcgttc ggtgctcatg tcgaggcatt cggcgcagcg ggtctgcgtc cctagggcgc
     7141 cgcctccgtc aggggatgtg ggccaccatc tcgatttcga cagtcagctc ggggcgcgcc
     7201 agcgcggcca cgccgatcgt gctgcgcgcg ggcagatccc ccgtgaagta cgatcggtag
     7261 acttcgttca tggcggcgaa gtctttcatg tcggtcaaga agaccgtcac cttcagcaca
     7321 tggtcgaatc ccgatcctgc ggcctccagc acggcgcgca aattctccat cgtgttgcgg
     7381 gtctgctcgc gaaagtcggg gggcaccttg cccttggcgt ccatgccgac ggcgcccgcg
     7441 gtgaacagca ggttcccgaa gcggatcccc ggtgaatacg gggcgagcgg agcggcacgg
     7501 cccgtgggga acacactctg acggttcatg acccacctcc tcgaaaatgt cgaccgacgc
     7561 tggggataat tccccgcggg tcgcgcctga cctcgtgggg tcaggggtag tgccggccca
     7621 actccgaggc gtacgtcctc ccgagccgtg ggccgtgctc gtcgagccag tcgcggaggc
     7681 gccgccgccc caccggcgcg cctcgggaca cgagcaggtc ggacatcagg cccgtgatct
     7741 cgtcgggggt gaggatgacg tcgtgcacca ggcggcccgc cattcccaag aggatgcgta
     7801 cgaggcccgg aggcaggtgg acgatcctgg cccggctccg gatcgcaccg gcgatgagcc
     7861 gcacgagctc atcgaacgtg aacgtctcgg gaccgacgac atccaccacg aagttctccg
     7921 cgcgctcggc cgcgctcacg gcgacctcgg cgacgtcctc gacgtcggcg cactgcagcc
     7981 ggtacccgcc gtcgtccggg atgacgaagg ccggaaaatg tcgcaggaac cacgcgatat
     8041 tgttgagcag gatgtccccg gggccgtaga tcagccccgg ccgcaggatc gcgtacgaga
     8101 gcgcggattc gcggatggcc cgctcgagga tccccttccc ccggaagtac gggagcgggg
     8161 aatcctcgga ggcgctggtc acgctgaggt gcacgatccg gcggatgcgc gccgcctcgc
     8221 aggcccggag gagggtccgg gtgttcgcga tggcctcggc aaacgtcgcc cggccatacg
     8281 gaaagcgcac ccagtacgta ttgtagagga cggccgcccc ctccaggctc cgcgccaact
     8341 cgtccgggtc gtcgaagcgg tacgcggcca cgcgcacccg ctccccgaac gggttcgggc
     8401 gatcgggacg gcgggtgagg gtcttgaccc gcctcccctt cgagaggagg cggcgggtga
     8461 tggcgcctcc ggtaaagcca aacgctccgg ttacgacgtc gagcgtttcg gtctccacga
     8521 tgcgcggctc gggaccgcgg cggcgcacgg tcggacggcg gagcggggcg ggggccgcgg
     8581 ctacgccggc gcgcctccgg ccatggcggg cacgatggac acctccgccc catccttcag
     8641 gggggtccgg agcccggaga ggaaccggat gtcctgttcg tcgacgaaga tgttgacgaa
     8701 ctggagcacc tcgccccgat cgtccaggat cttcgcccgg atccccggaa accgccggtc
     8761 gagatccgcg agcgcctgcg ccaggtcgct cccatcgacc gtcaccaccc gctccccatt
     8821 cgtgatccgg cggagcgggg tcggaatgcg caccgacacg gacatcaaag gcgcctcccg
     8881 cgtcccctag ccgcccgcga gcgcgagcac gtgcttctcg aacgacgcga gcgtcggttt
     8941 gatgctgacc gcggcctcca ccacctcttc cacggcctcg agcgccttca ggccgatgcc
     9001 ggtgatgtac gccaccgtga cctcgtcggg cccgatcacg ccggaggcgg ccagcttgcg
     9061 gagcacgccg accgtgaccc ccccggccgg ctcggtgaag aggccctcgg tctgcgcgag
     9121 caggcggatc gcgtcgaccg tctcctcgtc ggtgacggac tcgacggcgc ctccggtgcg
     9181 gcgggcgagg tcgaggacgt aggcaccgtc ggccggcgcg ccgatcgcca gggacttcgc
     9241 gatcgtgttc ggccggaccg ggaggacctt gtcggtcttc tgcttgtacg ccgtcgcgat
     9301 cgggccgcag ccctctgcct gggccgcggt catccgcacc gattggcggg ggatgacccc
     9361 gagccgctcg aactcctcga agcccttgtg gatcttgacg aacatgttcc ccgaggcgac
     9421 ggggacgatg acccggtcgg gcgcccgcca cccgagttgc tcggcgacct cgaacgcgag
     9481 ggtcttggcc ccctccgagt aatacggccg caggttgacg ttggcgatgg cccagcggtg
     9541 ctcgtccccg atctcgaggc agaggcggtt cacatcatca tacgtgccgt cgacctccac
     9601 caccgttgga cggtagaccg aggtcgtgat gaccttggcc cgctcgagcc ccttcgggat
     9661 gaacacgacc gcgcgc
//
LOCUS       DASQGH010000232         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35010, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000232 DASQGH010000000
VERSION     DASQGH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35010"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VEW91_04450"
     CDS             <1..1160
                     /locus_tag="VEW91_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM90868.1"
                     /translation="PEEHKLGLRGSSTTSLFLDNVRIARDRVLGEVGRGHVVALNILN
                     LGRLKLGAGCVGAAKHALRDAVAYACERRQFGRPIASFGLIKQKIAEMVIRLYLSESA
                     VYRVGGLVDQALGGIDLTGDGGGREAARALEEYAPECAINKVLGSEMLDLVVDEMVQI
                     YGGYGFIEDYPAARAYRDARINRLYEGTNEINRLVIAGQLLRRGRAGRIPLLDAARRA
                     ATLLDAAADDQPAPLPAPGSPDEVGAISGEFLARAKTVVLACLGRAVDRAGADLDEHQ
                     EILGWLSDQLIEVFAVESGVLRARQGAGVHGGSLMAALARDAVEELGPSLERFATRIL
                     SAVEDGPRLARDLAGARVLLRCRRQNPLATRRAIADQVLEAGGYGLGLPPA"
     gene            1392..2969
                     /locus_tag="VEW91_04455"
     CDS             1392..2969
                     /locus_tag="VEW91_04455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYM90869.1"
                     /translation="MPTHIGQDPAPYRPLWLRMRARTSEEWISLVRVVLLLALIPALW
                     FGVIRVSQPAVNASIMLFGAYVIVLALGPRWLRALRRADLTIALDILVVTLVVIISGN
                     LASPFLYLYYLTILQAAARLNLRQAIAAALAMAGMIVLLWTHAEVTALETAGFRLGAI
                     IAGGFFLALFLSMLVQEYRATQDRVLWADTLDQRLKEATKQLEEQLQELRFYNELAGR
                     LSGELRVEGVLEILLEVFLPTVGLSEGVAYILGEDEAPHFATARGFDRAEGDHDAGTL
                     SFPALPAGAVGGEVLLHMSDGSEGAPARRMACVPLVRAGSLRAWLGGLGDVPESFPEH
                     VLRRLRGIATQGVSALEAARLHEEVQRMLSVNPNRSLYPWNGLQKLVAEEIRRSTELM
                     LVFSLAEIQLEDYGSTSWIEDQDRDLALRRVVKLLQASLRRVDVASHDGAGRFVLLLA
                     RLPKIQSVEVLKRLTQKLEEDSVAARLLEVPRITVTAGVVTFPEDGSTASALLDKLRE
                     LVAQGPSTPARVHMPNS"
BASE COUNT          456 a         1037 c         1076 g          533 t
ORIGIN      
        1 gcccggaaga gcacaagctg ggcctgcgcg gatcgtccac gacctcgctg ttcctcgaca
       61 acgtccggat cgcccgtgac cgcgtgttgg gcgaggtggg tcgcgggcac gtggtggccc
      121 tcaacatcct caacctgggc cggctcaaac tcggggccgg gtgcgtcggt gcggccaagc
      181 acgccctgcg cgatgcggtg gcctacgcat gcgagcggcg ccagtttggc cgcccgatcg
      241 cttcgttcgg gctcatcaag cagaagatcg cggagatggt gatccgcctc tacctgtccg
      301 agagcgcggt ctaccgggtg ggtggcctgg tcgaccaggc gctcggcggc atcgatctga
      361 cgggcgacgg tggaggccgg gaggctgcgc gggccctcga ggagtacgcc ccggagtgcg
      421 cgatcaacaa ggttctcggg tcggagatgc tcgacctggt cgtggacgag atggtgcaga
      481 tctacggcgg gtacgggttc atcgaggact accccgcggc ccgggcctac cgcgacgccc
      541 gcatcaaccg cttgtacgaa gggacgaacg agatcaatcg cctggtgatc gccggccagt
      601 tgctgcgccg cgggcgcgcg gggcggatcc cgctcctgga tgccgcgcgg cgcgcggcca
      661 ccctcctcga cgccgcggcc gatgaccaac ccgcaccgct ccccgccccc ggcagcccgg
      721 atgaggtggg cgcgatctcc ggggagttcc tggctcgggc caagacggtc gtgctggcct
      781 gtctgggccg ggccgtcgac cgggccggtg ccgacctcga cgagcaccag gagatcctcg
      841 gctggctcag cgaccagctc atcgaggtgt ttgccgtcga atcaggggtg ctgcgggccc
      901 ggcagggagc gggggtccat ggaggaagcc tgatggcggc gctcgcccgc gacgccgtcg
      961 aggagctggg accgtccctt gagcggttcg ccacccggat cctctccgcg gtcgaagacg
     1021 gcccccggct ggcgcgcgac ttggccggcg cgcgcgtcct gttacggtgc cgccgccaga
     1081 atccgctggc cacccgccga gcgattgccg accaggtcct cgaggcgggt gggtacggtc
     1141 tcgggctccc gcccgcgtag cccgccagga cgacccacag cggactcgat gccccgagag
     1201 gccctgggcg gcccgagggc gcgccgggcc ctggggccgg ccgcaccctc ccccaacatg
     1261 cacccgacct tgtcctagat gacctgcaaa tggggcatgt tcgtatttgt caagctgcga
     1321 gtgggtccat gcccgcgccg gcccacgcgg ttggagtcca ggctgtccga gctccgaggc
     1381 aaggaggcct catgcccacc cacataggtc aggatccggc tccgtaccgg cccctctggt
     1441 tgcgcatgag agcccgcacg agcgaagaat ggatcagcct ggtgcgggtc gtcctgttgc
     1501 tcgcgttgat ccccgcgctg tggttcgggg tcattcgcgt gtcacagccg gcggtaaacg
     1561 ccagcatcat gctgttcggg gcgtacgtga tcgtcctcgc gcttgggccc cggtggcttc
     1621 gggcgctgcg aagggccgat ctcaccatcg cgctcgacat tctggtcgtc accctcgtgg
     1681 tgatcatctc cggcaacctc gctagcccgt tcctctatct gtactacctg acgatcctgc
     1741 aggccgcagc ccggctcaac ctccgccagg cgatcgccgc cgccctggcg atggccggca
     1801 tgatcgtcct tctctggacg catgcggagg tgaccgcgct ggagacggcc gggttccgcc
     1861 tcggcgcgat catcgccggc gggttcttcc tggcgctgtt tctcagcatg ctcgttcagg
     1921 agtaccgcgc aacccaggat cgggttttgt gggcggacac tctggatcag cgtctcaaag
     1981 aagccaccaa gcaactcgag gagcaactgc aagaactgcg gttctacaac gaactggcgg
     2041 gccgcctctc gggcgaactt cgggtggagg gggtactgga aattctgctc gaggtctttc
     2101 tcccgaccgt cgggctctcc gaaggagtcg cgtacatcct gggtgaggac gaggctccgc
     2161 actttgcaac cgcccgaggc tttgaccggg cggaaggcga ccacgatgcg gggacattgt
     2221 ccttccccgc cctccccgcc ggagccgtcg ggggcgaggt gctgcttcac atgtctgacg
     2281 ggagcgaagg ggcgccggcg cggcgcatgg cgtgtgtgcc cctcgtccgc gcggggagcc
     2341 tccgggcgtg gctcggcggg ttgggtgacg tgcccgagtc gtttccggag catgtgctcc
     2401 ggcgccttcg cggcatcgcc acccaggggg tctcggcgct cgaggcggcg cggttgcacg
     2461 aagaagtcca gcggatgctc agcgtcaacc ccaaccgatc cctctacccg tggaacgggc
     2521 tccaaaaatt ggtcgccgaa gagatccggc ggtccaccga actcatgctg gtgttctcgc
     2581 tggccgagat ccagctggag gattacgggt caaccagttg gatcgaggac caggacagag
     2641 atctcgccct ccgccgggtg gtgaagcttc tccaggcctc gttgcggcgc gttgatgttg
     2701 cctcccacga cggggctgga aggtttgtgc tgctgctcgc gcgcctgccc aagatccagt
     2761 ctgtcgaagt gctcaagcgg ctgactcaga aactggagga ggactcggtc gccgcccggt
     2821 tgctcgaggt cccccgcatc acggtcaccg cgggtgtggt gacgtttccc gaggatggaa
     2881 gcacagcgtc cgcgctgctc gataagctca gagaactggt cgcgcagggc ccctcgaccc
     2941 cggcccgcgt gcacatgccc aactcgtgaa gaagatctcc tagcgtccct ccaggttttc
     3001 gctcctccgt gtagcccccc ggaggtaaga gcccgattcg tcgcccgtgt tgcctttaca
     3061 ggcgagggag gggggccgta tgctggcttt gtggcgggag tg
//
LOCUS       DASQGH010000233         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40655, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000233 DASQGH010000000
VERSION     DASQGH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40655"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VEW91_04460"
     CDS             complement(<1..207)
                     /locus_tag="VEW91_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HYM90870.1"
                     /translation="MTQPVGHLLFTLHTHLPFVLNHGRWPFGSDWLSEATVQSYLPLL
                     RVIERLAEEDITPTVTLSLSPVLCE"
     gene            complement(319..1158)
                     /locus_tag="VEW91_04465"
     CDS             complement(319..1158)
                     /locus_tag="VEW91_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYM90871.1"
                     /translation="MDPLEPLRRVGRHLLDPERLAGALESVILLAVIAGAAWVALRAS
                     VGVVRHTTTWRAGQPGARMAPLVEGLLRYAIVFAALILMLGAVHINITPVLASAGVLG
                     LVLSFGAQYIIRDLLAGVFLISEGIIQAGDVVRLDGDVGTVERVSLRSTQIRMFSGEL
                     LTVPNGAIARIGNLSRDYARAIVQILIPYTQDAGAALEALRDAGRAWAATQARDEQVE
                     PSVDGIVGLRDAGAVLQLSVVVPPGRREAVAANLRRQAMEALARRGITPGTAAVWTPP
                     PGK"
     gene            complement(1161..>1917)
                     /locus_tag="VEW91_04470"
     CDS             complement(1161..>1917)
                     /locus_tag="VEW91_04470"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HYM90872.1"
                     /translation="RRLGIPTYHHAEMLPEAGAYGGKGEALWKSLHVLRGDLIVWVDT
                     DIRNIHPKFVYGLLGPLLRDASIGYVKGFYRRPIQVGRRRYESGGGRVTELTARPLLN
                     LFFPELSGVIQPLAGESAGRREVLERLPFFTGYGVEIGLLIDVLARFGLGRIAQVNLG
                     SRVHRNRELPSLSVMAFAIAQVLMTRVGESAGAPIAEQMNRTVTLVGREDGLSLERHD
                     VREHERPPIASVPAYRQRRGLPNGAREAAAGDG"
BASE COUNT          304 a          688 c          664 g          261 t
ORIGIN      
        1 ctcgcagagc accggcgaca ggctgagggt gaccgtcggg gtgatatctt cctccgcgag
       61 ccgctcgatg acccgcagga gcgggaggta ggactggacc gtcgcctcgg agagccagtc
      121 gctgccgaac ggccatcggc cgtggttcaa cacaaacggg aggtgggtgt ggagggtgaa
      181 gagcaggtgg ccgacgggct gggtcatcgc gtccctacag gctcgagcgg atcggcggga
      241 aggatcgggg agcgccgtcc ttgccctgat tctaccctaa gaatgccccg ttgtcccggc
      301 actcttagcg acgcctcgct acttgcccgg cggaggcgtc cagaccgcgg ccgtcccggg
      361 cgtgatgccg cgccgtgcca acgcctccat ggcctgccgc cggaggttcg ccgcgaccgc
      421 ctcccggcgt ccggggggga cgaccaccga tagctgcagc accgccccgg cgtcccggag
      481 ccccacgatg ccgtcaaccg acggctcgac ctgctcgtcg cgcgcctggg tggccgccca
      541 cgcccgcccc gcgtcgcgga gcgcctcgag cgccgcgccc gcatcctgcg tgtacgggat
      601 caagatctgg acaatcgccc gcgcgtagtc acggctgaga ttcccgatcc gcgcgatcgc
      661 gccgttgggg accgtgagga gctccccgga gaacatgcgg atctgggtgg accggagtga
      721 cacccgctcg acggtcccga catccccgtc caatcgcacg acgtcgcccg cctggatgat
      781 gccctccgag atgaggaaca ccccggccag caggtcacgg atgatatact gggcgccgaa
      841 gctgaggacg aggcccagga ccccggcgct cgcgagcacg ggggtgatgt tgatgtgcac
      901 cgcgccgagc atcaggatga gcgccgcgaa gacgatcgca taacgcagga gtccctcgac
      961 gaggggcgcc atccgcgcgc ccggttggcc ggcccgccag gtcgtggtgt ggcgcacgac
     1021 ccccaccgag gcgcgcagcg ccacccacgc ggctccggcg atcactgcaa gcaagatcac
     1081 ggactcgagc gcgccggcga gccgctcggg atccaggagg tggcggccca cccgccgcag
     1141 gggttcgagc gggtccacgg tcagccgtcc ccggcggccg cctcgcgggc accgttgggt
     1201 agcccccggc gctgccggta ggcggggaca ctcgcgatgg gcggccgctc gtgctcgcgc
     1261 acgtcgtgcc gttcgaggga caggccgtcc tcccggccga ccagcgttac cgtccggttc
     1321 atctgctcgg cgatgggcgc gcctgcggat tctcccaccc gcgtcatcaa cacctgggcg
     1381 atggcgaagg ccatgacgga cagcgagggg agttcccggt tgcggtgcac gcgcgacccc
     1441 aggttcacct gcgcaatccg cccgagcccg aaccgcgcca ggacgtcgat caggagcccg
     1501 atctcgaccc cgtagcccgt gaagaacggc agacgctcga gcacctcccg gcgtccggcg
     1561 gactcaccgg ccagcggctg gatcaccccc gacagttcgg gaaagaacag gttgagcagc
     1621 gggcgcgccg tgagttcggt gacccggcca ccccccgatt cgtaccggcg gcgccctacc
     1681 tggattggcc gccggtaaaa gcccttgacg tacccaatgg acgcgtcacg caacagcggc
     1741 cccaggaggc cgtagacgaa cttgggatgg atgtttcgga tgtccgtatc cacccacacg
     1801 atcaggtccc cccggagcac gtgcaggctc ttccaaagcg cctccccctt cccgccgtag
     1861 gcgcccgctt cgggcagcat ctcggcgtgg tggtacgtgg ggatgccgag gcggcgg
//
LOCUS       DASQGH010000234         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54816, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000234 DASQGH010000000
VERSION     DASQGH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54816"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VEW91_04475"
     CDS             complement(<1..264)
                     /locus_tag="VEW91_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90873.1"
                     /translation="MLYTGERGRLVYPKELRYSTEHEWAKVEGRRVRVGITKFAADRL
                     SDVVYVELPKVGTEVAFMQPFGVVESVKAVSDLYAPVSGKVVEI"
     gene            complement(234..1343)
                     /gene="gcvT"
                     /locus_tag="VEW91_04480"
     CDS             complement(234..1343)
                     /gene="gcvT"
                     /locus_tag="VEW91_04480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYM90874.1"
                     /translation="MDAPLKRTPLHAAHLALGARMVPFGGWEMPVQYSGIIDEHQAVR
                     TRAGLFDVSHMGEVDVRGPGAGPLIQRLVTNDLSRVAVGQAMYTPICTPEGGIIDDLL
                     VYRLGDEHLMLVVNASNTAEVLAWVREHAPGTVQIIDRTSEIALLALQGPRAQGILER
                     LTSAPLGTIRYYRFGDGVEVAGQRALVSRTGYTGEDGFEIYVDGGGAVHVWNALLEAG
                     ERAGILPAGLGARDTLRLEAGYLLHGNDMDKSTTPLEVGLGWTVKLGKGEFIGARALE
                     RQQREGLSRRLVGFTLRERAIARHGFPIRQDGKPIGQVTSGSFGPTVEKSIGLGFVPP
                     VHADPGQRIAVEIRGRAIEGTVTKLPFYTRGKGAG"
     gene            complement(1344..1994)
                     /locus_tag="VEW91_04485"
     CDS             complement(1344..1994)
                     /locus_tag="VEW91_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HYM90875.1"
                     /translation="MRFQDRRDAGEQLARQLGTLGLGPATVLAIPRGGVIVADAIVRT
                     FGWPLDVMLPRKLRAPRNPELAFGAVTEDGTIFLDARLARTLHVTDEYLREEIADQIA
                     EITRRRLAYRGTRPEPDLRSRTAVLVDDGLATGATAIASVRMLRHRLAREVSVAIPVG
                     PPEAVRRLEAEADRVICLVRPTEFTAVGEFYEDFRQTGDEEVVACLTQAWGRTEVS"
     gene            complement(2007..2471)
                     /locus_tag="VEW91_04490"
     CDS             complement(2007..2471)
                     /locus_tag="VEW91_04490"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HYM90876.1"
                     /translation="MTTHSDAQAWLVVLILLAVLGGVIILFALAQGLRSRSAPAAGTM
                     AADESHRRELAEISARLAALEQGMARVVDALPRSIQGVGVVRYNPFPDTGSNMSFSLA
                     LLDGRASGVVVSVLTNRDGSRVYGKAVENGRSSYPLSDEERQALALAQGQRA"
     gene            complement(2577..>3018)
                     /locus_tag="VEW91_04495"
     CDS             complement(2577..>3018)
                     /locus_tag="VEW91_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYM90877.1"
                     /translation="SDRGERFVLEKNGYLEPALIGLTHHRERLTPTVMAAYRAPFPTP
                     PSRRALLCWSRDIAVAETDPSYPEMKRIEARLALFAQTPILLVWGMQDPVLSPPVLRW
                     WQTRYPQAVTREIEDAGHFLQEDAPEQTVGWIEQFLDVPPQPGA"
BASE COUNT          453 a         1098 c         1020 g          447 t
ORIGIN      
        1 gatctccacc actttcccgg agaccggcgc gtacagatcg ctcacggcct tcaccgactc
       61 cacgaccccg aacggctgca tgaacgcgac ctcggtcccc actttgggca gctccacgta
      121 caccacatcc gagagccggt ccgccgcgaa cttggtgatg cccacccgga cccgccgccc
      181 ttcaaccttc gcccactcat gctccgtgga gtaccgcagc tctttggggt acactagccg
      241 gcccctttcc cccgtgtata gaatggcagc ttggtgaccg tgccctcgat cgcccggccg
      301 cggatctcca ccgcgatgcg ctggccgggg tcggcgtgca ccggggggac gaatccgagc
      361 ccgatgctct tctccactgt gggcccgaag ctcccgctcg tcacctgccc gatgggcttc
      421 ccgtcctggc gaatggggaa cccgtggcgc gcaatcgccc gctcccgcag cgtaaacccc
      481 acgagccggc gggagagccc ctcgcgttgc tgccgctcga gggcgcgggc gccgataaac
      541 tcgcccttgc cgagcttgac ggtccagccc agcccgactt cgaggggcgt ggtggacttg
      601 tccatgtcgt tcccgtggag gaggtacccg gcctcgagcc ggagcgtgtc gcgcgccccg
      661 agacccgcgg ggaggatccc cgcccgctcc ccggcctcga ggagggcgtt ccacacgtgg
      721 acggcgcccc cgccgtcgac gtagatctca aacccgtcct ccccagtgta ccctgtgcgc
      781 gacaccaggg cgcgctggcc ggcaacctca accccgtcgc cgaaccggta gtaccggatc
      841 gtccccaggg gcgccgaggt gaggcgctcg aggatcccct gggcgcgcgg cccctggagg
      901 gccagcaggg cgatctcgga ggtgcgatcg atgatctgga cggtccccgg ggcgtgctcg
      961 cggacccagg cgagcacttc ggcggtgttt gacgcgttga cgacgagcat gagatgctcg
     1021 tcccccagcc ggtacaccag cagatcgtcg atgatccccc cctcgggggt gcagatcggc
     1081 gtgtacattg cctgccccac cgcgacgcgg ctgagatcgt tcgtcacaag ccgctggatg
     1141 agcggccccg cccccggccc cctgacgtcc acctcgccca tgtgggacac gtcgaagagg
     1201 ccggcgcgcg tgcggaccgc ctggtgctcg tcgatgatcc ccgagtactg cacgggcatc
     1261 tcccacccgc cgaacgggac catccgggcc cccagggcga ggtgcgcggc gtgcagcggg
     1321 gtgcgcttca gcggtgcgtc cattcacgaa acctcggtcc ggccccaggc ctgggtgaga
     1381 caggccacca cctcttcatc gccggtctgg cgaaagtcct cgtagaactc tccgacggca
     1441 gtgaactccg tcggccgcac gaggcagatg acccgatccg cttccgcctc caggcgccgc
     1501 acggcttcgg gcgggccgac cgggatcgcg accgagacct cacgggccag gcgatgccgc
     1561 agcatccgga ccgacgcgat cgcggtcgca ccggtggcga gaccgtcgtc gaccagcacg
     1621 gcggtccggg atcgcagatc cggctcgggc cgggtcccgc ggtacgcgag gcggcgccgg
     1681 gtgatctcgg cgatctggtc ggcgatctcc tcccggagat actcgtcggt gacgtgcagg
     1741 gtgcgcgcca gccgcgcatc gaggaagatc gtgccgtcct cggtgacggc gccgaacgcg
     1801 agctcggggt tgcgcggggc ccggagcttc cgggggagca tcacatcgag cggccagccg
     1861 aaggtgcgca cgatcgcatc cgccacgatg accccgcccc gcggaatcgc gaggaccgtc
     1921 gccggcccga ggcccagcgt gcccagttgg cgcgcaagct gctcgccggc gtcccggcgg
     1981 tcctggaacc gcatcgctac gggatgttac gcgcgctgtc cttgggcgag cgccagcgct
     2041 tgacgctctt catcggaaag cgggtagctc gaccgtccgt tttccaccgc cttgccgtag
     2101 acccgcgacc cgtcgcgatt ggtcagcacg ctgacgacga ccccgctggc acgtccgtcg
     2161 aggagcgcaa ggctgaagct catgttgctc cccgtgtcgg ggaacgggtt gtaccggacc
     2221 acgcccaccc cttggatcga ccgggggagg gcatcgacga cacgggccat gccctgctcg
     2281 agcgccgcca gccgggcgct aatctctgca agttcccggc gatgcgattc atcggccgcc
     2341 atcgtccccg ccgccggggc gctccgcgag cgcaggccct gggcaagcgc aaacaggatg
     2401 atcaccccgc cgagaaccgc gaggaggatg agcaccacaa gccaggcctg cgcatcgctg
     2461 tgcgtcgtca agacggggag cctccgcggc gtgctccagc cattttcttt gccggggcgg
     2521 gggttccctt cgcatcgcgc gcggcggccg gcgcggacga cgcggtcccc gcgcactcac
     2581 gcacccggct ggggtggaac gtccaggaac tgctcgatcc atcccacggt ctgttcgggc
     2641 gcatcctcct ggaggaagtg gccggcatcc tcgatctcgc gggtcaccgc ctgaggatag
     2701 cgcgtctgcc accagcgcag gaccggggga gacagcaccg gatcctgcat cccccagacg
     2761 aggaggatcg gggtctgcgc gaacagggcc aggcgtgcct cgatccgctt catctccgga
     2821 tacgatggat cggtctcggc caccgcaata tcccgcgacc agcacagcag cgcgcggcgg
     2881 gatgggggcg tcgggaacgg cgcgcggtag gctgccatga cggtcggggt caggcgttcc
     2941 cggtggtggg tgagcccgat cagggcgggc tccaggtagc cgttcttctc gagcacgaag
     3001 cgctcgcctc ggtcggac
//
LOCUS       DASQGH010000235         1962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35018, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000235 DASQGH010000000
VERSION     DASQGH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35018"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VEW91_04500"
     CDS             <1..588
                     /locus_tag="VEW91_04500"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HYM90878.1"
                     /translation="ATPAVAAEIYRIIHRYEAVVRAHGEDIRGANPAPGNIEGGLTTI
                     EEKSLGAAKKGGTRTIQEVVEYAVRPTAHGLVVMDTPGNDIEQMVGMVAAGAQVAAFT
                     TGRGTPTGSPIAPVIKIATNSHTAHRMQDNIDLNAGLILEGRETLETMGRRIYEALLA
                     VAQEEPTKAERLGHREFSISRYPFAVLDAPAATSR"
     gene            660..1962
                     /locus_tag="VEW91_04505"
                     /pseudo
     CDS             660..1962
                     /locus_tag="VEW91_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
BASE COUNT          315 a          683 c          691 g          273 t
ORIGIN      
        1 gcgacccccg cggtcgccgc ggagatctac cggatcatcc accgctacga ggcggtggtg
       61 cgcgcccacg gcgaggatat ccggggcgcc aacccggcgc cagggaacat cgagggcggc
      121 ctcacgacca tcgaggagaa atcgctcggc gcggccaaga agggcggcac gcggacgatc
      181 caggaggtcg tggagtacgc cgtccggcct acggcgcacg ggctggtcgt gatggacacg
      241 cccggcaacg acatcgagca gatggtgggg atggtggccg cgggggcgca ggtggccgca
      301 ttcacgaccg gccgggggac gcccacgggg agcccgatcg ccccggtgat caagatcgcg
      361 acgaactccc acacggcaca ccgcatgcag gataacatcg acctcaacgc cggcctgatc
      421 ctcgaggggc gggagacgct cgagacgatg ggccgccgca tctacgaggc gctcctggcg
      481 gtcgcccagg aggaacccac gaaggcggag cgcctcggcc accgcgagtt ctcgatcagc
      541 cgctacccgt tcgccgtcct ggacgcaccg gcggccacct ctcgatagcg tcaggcgaag
      601 gagggtccgc gatggcccgg aacgggaagg tatcgctcac gcacgacatc ccggtgcgaa
      661 tgcgcgacgg cgtgaccctg tacgccgacg tgtacacgcc ggagggcgat ggggtgttcc
      721 cggtgctgct catgcggact ccctacggca agacccaggc ccaggactcc atccaggccc
      781 accccctgtg gtacgctcgc cgcggataca tcgtcgccgt ccaggacgtc cgaggccggt
      841 ggagcagcga gggggagtgg tacccgttcg cccactaggc cgaggacggc tacgactcgg
      901 tcgtgtgggc cgcccacctg ccccgggcca acgggcgggt gggcatgtac gggatctcct
      961 atgtcggcgc gacccagatg ctcgccgcgg cggccgcgcc gccccacctg gcgtgcatcg
     1021 ttcccgggat gacgtcttcc gaatactacg acggctggac gtaccgcgcg ggcgccctcc
     1081 acctcgcctt caccgagtcc tgggcggtga tgctggccca ggacaccgcc cgccggcgcg
     1141 agctccgcga actcgaggcg gatctccacg cgcgcttcag cgcggccgag ctcgggttcg
     1201 ggaccctgcc cctcaagggg cacgagctgc tccgcaggga cgccatcgct ccgtggttct
     1261 tcgactggct cacgcatccc acgcgcgacg agtactggga gcggtggagc atcgagcgcc
     1321 ggcacacgaa catccgcgtc ccggcgtttc acgtcgccgg gtggtacgac atctttctgg
     1381 acgggagcct ccggaactac ctggggctgc gcgagcgcgc cgcggacgac cgcgcacgca
     1441 ccgggcagcg gatcctgatc ggtccctggc accacgtccc ctgggcaccg atggtctccg
     1501 ggtgggactt cggccccgag gcgcggagcc aaatcaacga ctggcaggtg cgctggttcg
     1561 attactggct ccggggcgtc gtgaacggcg tccccgacga tccgccggtg cgcctcttcg
     1621 tgatgggcga gaactggtgg cgcgaggaac gcgagtggcc cctcaggcgg gccgagagca
     1681 ccgagttctt cctgcacagc cagggcactg cgaactcgct caatggggac ggcgtcctca
     1741 gccgggatcg gtgcggcgat gaggagcccg atatcttcat ctacgatcca cgatcacccg
     1801 cgatgagcct gggagggcgg tcctgctgcc ggtacctggt ctcgccgatg ggcccggccg
     1861 accaaaggcc ggcggagatc cacaccggtg tgctcgtcta caccaccccg ccgctccagc
     1921 aggacctcga ggtgaccggg ccggtgtcgg cggtggtgtt tg
//
LOCUS       DASQGH010000236         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12470, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000236 DASQGH010000000
VERSION     DASQGH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12470"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VEW91_04510"
     CDS             <1..606
                     /locus_tag="VEW91_04510"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HYM90879.1"
                     /translation="TGIVEGTGVVRAAGGGGGPVRLRIEAGYDIRGVKVGDSIAVSGT
                     CLTVAAIAGGAFEADLTAETTARTTLGRLRPGDRVNLERPVAVGDRLGGHVVQGHVDA
                     VGRVVRSDPQGDASWLEIDAPASVARYIVEKGSVAVDGVSLTVARCDGNRFVVCLIPH
                     TCATTTLGALGPGAPVNLEADILAKYVERLLERERVTEAER"
     gene            603..1835
                     /locus_tag="VEW91_04515"
     CDS             603..1835
                     /locus_tag="VEW91_04515"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HYM90880.1"
                     /translation="MTMGAGEVQTAGFATIPQAVEAIRGGGFVIVVDDEARENEGDLL
                     LAAERVTPQALNFMLKHARGLITVPMTGGRLDALELPQMVAQNTSHQGTAFAVSVGAR
                     HRITTGISAHDRAATIQALVDPRTRSEDLSRPGHVFPLRATPGGVLRRAGHTEAATDL
                     AALAGLHPAGVLCEVMSDDGTMARLPELMGFAARHHLPIISIADLIRYRLERDRFVVC
                     EGRTRLPTPLGEFTVAVYQNTLDGATHLALIRGDLGGAEPVLVRMHSECLTGEVFGSL
                     RCDCGEQLHRALEAISREGRGVFVYIRQEGRGIGLANKIRAYALQDAGKDTVEANELL
                     GFAPDPRDYGVGAQILADLGLRRIRLLTNNPAKRVGLEGYGIEVVSRVPIEVAPNPEN
                     YRYLDAKRHKLGHLLSFE"
     gene            1847..2308
                     /gene="ribH"
                     /locus_tag="VEW91_04520"
                     /pseudo
     CDS             1847..2308
                     /gene="ribH"
                     /locus_tag="VEW91_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
     gene            complement(2337..2576)
                     /locus_tag="VEW91_04525"
     CDS             complement(2337..2576)
                     /locus_tag="VEW91_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90881.1"
                     /translation="MTIPLTKACPQCEGEMELLTADDTGEVVVYECPDCGYQVENRVD
                     DSEEESLGRQDLNGVLPTGGDDDADAGGPEPIDES"
     gene            complement(2718..3326)
                     /locus_tag="VEW91_04530"
     CDS             complement(2718..3326)
                     /locus_tag="VEW91_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HYM90882.1"
                     /translation="MGKYEARKFRAFEIELAGISKKTMEELYKLFQGYVNKANEILDR
                     LASGGVDLSKANPTYSEIRELKVELSRAIGGVKNHEVYFEHLGGKGGQPGPALTGLIT
                     KSFGSLGAWQADLKATAIAARGWAWTAYDWDAGSLLNFIGDEQNTYPIWNGTPLVALD
                     CFEHAYFIDYGTGKAAYIDTFFKLLDWDAVQRRAEHFQILKK"
     gene            complement(3409..3636)
                     /locus_tag="VEW91_04535"
     CDS             complement(3409..3636)
                     /locus_tag="VEW91_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90883.1"
                     /translation="MARLMLSVRCAACGEVFDTGIRIDRRNFARATLASNYHTCPRCH
                     TRGTYHKEEYLLHEEPPGRPETLAPPRNPDV"
BASE COUNT          510 a         1270 c         1344 g          584 t
ORIGIN      
        1 accggcatcg tagaggggac gggggtggtg cgcgccgccg ggggcggggg cgggcccgtc
       61 cggcttcgca tcgaggccgg atacgacatc cggggcgtca aggtcgggga cagcatcgcc
      121 gtcagcggca cgtgcctcac cgtggccgcg atcgccggcg gcgccttcga agccgatctc
      181 acggctgaga cgaccgcgcg gacgacgctc ggccggcttc ggcccggcga tcgtgtgaac
      241 ctggaacgcc cggtcgccgt gggggatcgc ctgggggggc acgtggtcca gggccatgtc
      301 gacgccgtgg gccgcgtggt gcgcagcgat cctcaggggg acgcgtcgtg gctcgagatc
      361 gacgctcccg cctcggtggc gcggtacatc gtcgagaagg ggtcggtcgc cgtggacggc
      421 gtcagcctga ccgtcgcgag gtgcgacggg aaccggtttg tcgtctgtct gattccccac
      481 acgtgcgcca ccaccaccct cggggctctc gggcccggcg ccccggtgaa cctcgaggcc
      541 gatatcctgg ccaagtacgt ggagcgcctg ctggagcggg agcgcgtgac ggaggcggaa
      601 cgatgaccat gggcgcagga gaggtgcaga ccgcagggtt cgccacgatt ccccaggcgg
      661 tcgaggcgat ccggggcggc gggttcgtga tcgtcgtcga cgacgaggcg cgggagaacg
      721 aaggcgatct cctcctcgcc gccgagcggg tgaccccgca ggcgctcaac ttcatgctga
      781 agcacgcccg cggcctgatc accgtgccga tgacgggcgg gcggctggat gcgctcgaac
      841 ttccccagat ggtggcgcag aacacctcgc accaggggac ggcgtttgcg gtctcggtgg
      901 gtgcccggca ccggatcacg accgggatct ccgcgcacga tcgcgcggcg acgattcagg
      961 cgctcgtgga cccccgcacc cgctccgagg atctgagccg gcccgggcac gtgttccccc
     1021 tgcgggcaac ccccggcggc gtcctgcgcc gcgccggcca caccgaagcc gccaccgatc
     1081 tggccgccct cgcgggcctc cacccggccg gggtgctctg cgaggtcatg agcgacgatg
     1141 gcacgatggc ccgcctcccc gagctgatgg ggttcgcggc ccggcaccac ctgccgatca
     1201 tctcgatcgc cgacctgatc cgctaccggc tcgagcggga ccggttcgtg gtctgcgagg
     1261 ggcggacccg gctgccgacc ccgctgggag agtttacggt cgccgtctat cagaacaccc
     1321 tggacggcgc gacccatctg gcgctgatcc gcggagacct cggtggggcg gagccggtgc
     1381 ttgtgcggat gcactcggag tgcctgaccg gcgaggtctt cgggtcgctg cggtgcgact
     1441 gcggagaaca actccaccgc gcgctcgagg cgatcagccg ggaaggccgc ggggtgttcg
     1501 tctacatccg tcaggaaggg cgggggatcg gcctcgccaa caagatccgg gcgtacgccc
     1561 tgcaggacgc cgggaaggac accgtggagg cgaacgaact gctgggcttt gcccccgatc
     1621 cgcgggacta cggcgtcggc gcgcagatcc tggccgatct cggcctgcgc cggatccgcc
     1681 tcctgaccaa caacccggcg aagcgcgtgg gcctggaggg gtatgggatc gaggtggtca
     1741 gccgggtgcc gatcgaggtc gcgccgaatc cggagaacta ccggtacctg gatgccaagc
     1801 gccacaagct cggccacctg ctcagtttcg agtgacgccg gcccccgtgg ctgaggcctt
     1861 ccggggcggg cgcgacggcg ccggcctccg cgtcgcggtg gcggtgagcc ggttcaacga
     1921 gcgcgtcacg cgccggctgc tccagggcgc cctgggagcg ctgcgccggt gcggcgtggc
     1981 cgaggagtcg gtggatgtgg cgtgggctcc gggcgcgtac gacctgcccg tgatcgccca
     2041 ggcgctcgcc cgctccggcc ggtacgacgc cgtcgtgtgc ctgggctgcg tgatccgggg
     2101 cgagacggcg cacgaccggt acgtcgcgct cggtgccgcg accggcctgc tccgggtggc
     2161 cctggagggt gccctcccgg tgacgttcgg cgtgctcacc gcccagacgc tcgagcaggc
     2221 ggaggcccgg agcggagggg cgcacggcaa caagggcgcg gacgcggcgc ttgcggcggt
     2281 ggaactcgcc aacgtcctgc gccagatcca ggcgtcccgg cgcggagatg ccggggtcac
     2341 gattcgtcga tcggctcggg tccccccgcg tccgcgtcgt cgtccccgcc ggtcggcagc
     2401 accccgttga ggtcctgacg gcccagcgac tcttcttcgc tgtcgtccac ccggttctcg
     2461 acctggtacc cacagtccgg gcactcgtac acgacgactt cgccggtgtc gtcggcggtc
     2521 aaaagctcca tttccccttc gcactgcgga caggccttgg tcaacgggat cgtcatcgcc
     2581 gcgccttgcc ccggcgtgct tgcccagggg cgtcctcccc agactaatgc ccgccttgcg
     2641 ggcccgctag cccgggggaa tgcgcgggag cccgggcggg gtcgcccggg ctcccggatg
     2701 accggccgac gcccggctta cttcttgagg atctggaaat gctccgcgcg ccgctggacc
     2761 gcgtcccaat ccagcagctt gaagaacgtg tcgatgtacg cggccttgcc ggtcccgtag
     2821 tcgatgaaat acgcgtgctc gaagcagtcg agcgcgacca gcggggtgcc gttccagatc
     2881 ggatacgtgt tctgctcgtc gccgataaag ttgaggagcg agcccgcgtc ccagtcgtac
     2941 gccgtccacg cccacccgcg ggccgcgatc gcggtggcct tgagatccgc ctgccacgcc
     3001 ccgagcgacc cgaacgactt tgtgatcagc cccgtgagtg cgggtcccgg ctggccgccc
     3061 ttgcccccga ggtgctcgaa atagacttcg tggttcttca cgccgccgat ggcgcgggac
     3121 agttccacct tgagctcacg gatttcggaa taggtcgggt tggctttgga gaggtcgacc
     3181 ccaccgctcg ccagacggtc gaggatctcg ttcgctttgt tgacgtaccc ctggaagagt
     3241 ttgtagagct cctccatggt cttcttgctg atgcccgcga gctcgatctc gaacgcgcgg
     3301 aacttcctcg cctcgtattt ccccatgccg tctccccctc tcggagtgtt ggagttggat
     3361 caccgcgcag gttgaagcag cggaaggtgc agcgtgaaac cggccccatt atacgtctgg
     3421 gttgcgggga ggcgcaagcg tctccgggcg gccggggggc tcttcgtgca gcaggtactc
     3481 ttccttgtgg taggtcccgc gggtgtggca ccgggggcag gtgtggtagt tgctggcgag
     3541 ggtcgcgcgc gcgaagttcc tgcggtcgat ccggatgccg gtatcgaaca cctccccgca
     3601 tgccgcgcac cgaaccgaga gcatgagccg ggccatgggg gttcaccccg ccggcggctg
     3661 ccccacggga tcatccccgc tgcggagtgc cgccacgtcg gcgcggcg
//
LOCUS       DASQGH010000237         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1052, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000237 DASQGH010000000
VERSION     DASQGH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(633..>1635)
                     /locus_tag="VEW91_04540"
     CDS             complement(633..>1635)
                     /locus_tag="VEW91_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C1A"
                     /protein_id="HYM90884.1"
                     /translation="GGYGLIGYGQCNIDSNSKLGLSLTNPDPWTKRRNHSGGMIESGD
                     GALHRNFELLACGPHSSFTHWWRENSSSTLPWAKAEVLGNDVACPPTFTGTTYNRNFE
                     TVYTTTNRRLHHWWYDQAGQRWNDGVVFGPTNVQGPVGFVESKYGPGNFEVVAALATE
                     KLEHWWRDGGGWHQGVTFGSGIAAAGHSLLQSTWDNLELVVVLADGRMQHWWRWDAGD
                     KAWYIGGTFGSGIASPPCMIQGQYGMANENGKGNFELCVALPNGTVQHWWRNNSAIGF
                     PWRMSATFGTDVVAVVALLEGSFGFNLEVVVQRQDHMLQHYWRDGAGWHPGVVIGPA"
BASE COUNT          259 a          540 c          554 g          282 t
ORIGIN      
        1 aaaggacgcc ccgacgtccg tggcgatcgc ctgaaggtca taggccagcg gcggagtctc
       61 ctcgaaggac tccgtcgtgg cggccggctt gtaggcgagc gacggggacg ccgagaccag
      121 ggtcacccgc acacgcttgg gcgccagcgc gcgcagggcg agcatggcct ccaccccacc
      181 gaacccgccg cccgctatca acacatcagc gcggtcagct aacgaggtgc tacgcggcat
      241 aggctggaac ttcgcatcgc cgtcgcccgc gcgcatccgt gaaatcccac gaagaaggcg
      301 acggttacta cgctgtctac gccggttcga tttcgcttac gtggacttcg atgtcgtggt
      361 gcatgagggg tgggcggggc ggtccgaagg gacgagcgag ctcgaggcgt accgtggtgg
      421 tcccggcacg cagggcttgg atcgccgcga gttggtcgct gctcccgccc cgtagctcgc
      481 ctgggcgtgg ctggagtggt gttgcggctg cccgcaccga cacctgcacg aggtcctccg
      541 gctcgctgac gcttgccgtc cagaggtagc cggccgaacc gtggccgggg aggctgaccg
      601 tggcctcctc gccgacggct agggcgagcg tgctaggcag gtccaatgac caccccgggg
      661 tgccagccgg cgccgtctcg ccagtagtgc tggagcatgt ggtcttggcg ctgcacgacc
      721 acctccaggt tgaagccgaa gctgccctcg aggagggcga ccacggccac gacgtcggtg
      781 ccgaaggtgg cgctcatcct ccaggggaag ccgatggcgc tgttgttgcg ccaccagtgt
      841 tgcacggtgc cgttgggtag cgcgacgcac agctcgaagt tgcctttccc gttctcgttg
      901 gccatgccgt actggccctg gatcatgcag ggtggtgagg cgatgccgct cccgaacgtg
      961 ccgccgatgt accaggcttt gtcgccggcg tcccagcgcc accagtgctg catccggcca
     1021 tcggcgagga cgaccaccaa ctcgaggttg tcccaggtgc tctgcagcag cgagtgcccc
     1081 gcagccgcga tcccggaccc gaacgtgact ccctggtgcc agccgccgcc atcgcgccac
     1141 cagtgctcga gcttctcggt ggccagggcg gcgaccacct cgaagttgcc gggcccgtac
     1201 ttgctctcga cgaatccgac gggtccctgc acattggttg gtccaaagac gaccccatcg
     1261 ttccagcgct gacccgcctg gtcataccac caatggtgaa gacggcgatt cgtcgtcgtg
     1321 tagaccgtct cgaagttgcg gttataggtt gtcccggtga acgtcggcgg gcaggccaca
     1381 tcgttgccca ggacctccgc tttggcccag gggagcgtag aggaggagtt ctcccgccac
     1441 caatgggtga acgagctgtg agggccacag gccaggagct cgaagttgcg atgcaacgcc
     1501 ccgtctccgc tctcgatcat cccgccgctg tggttgcggc gcttggtcca cggatcgggg
     1561 ttggtgagcg agagcccgag cttggagttg gaatcgatgt tgcactgtcc gtagccgatg
     1621 agcccgtacc cccca
//
LOCUS       DASQGH010000238         7296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46398, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000238 DASQGH010000000
VERSION     DASQGH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46398"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VEW91_04545"
     CDS             complement(<1..1476)
                     /locus_tag="VEW91_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYM90885.1"
                     /translation="MRLAPNPCSEGGIDLPAAIALPEGIVAVVKRDCPTCRLVVPVLT
                     QLRAAGAALTIYSQDDPGFPPGLPVVDDTGLDVSVGLQIETVPTLLRVVGGRVVERTE
                     GWMRDRWERLTRVGGLGDGLPAFRPGCGSRTMEPGIAERLAAREGAGRLRSRRIAIGE
                     AEDAIEACHARGWSDGLPVVPPTPERVLRMLEGTPRAPDEVVAVVPPDYAGCTVEKVA
                     INAVLAGCRPEYLPVVLAAVEAVCTDAFNIHGVLATTYFVGPVVVVNGPIAKAIGMNS
                     GVNALGQGNRANAAVGRALQLVIRNVGGGRPGEVDRATLGNPGKYTFCFAEDEDRSPW
                     EPLSVERGLPPGTSGVTVFAGEGPRGVFDQLSRTPESLSRSLAWCLRTVAHPKFVRDY
                     DALVIISPEHGRVFREAGWSKARLREEIVRVLTLPGAEIVRGAGGCAEGMPEKFAAGA
                     SPKFLPENLLLVHAGGTAGLFSAIIGGWKNGPAGSQPVTREV"
     gene            complement(1520..2668)
                     /locus_tag="VEW91_04550"
     CDS             complement(1520..2668)
                     /locus_tag="VEW91_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM90886.1"
                     /translation="MAPARFQFVLLWLLGADLRLTMLAVPPLLPRIHAALGLNETGVA
                     ALTSLPVLVLGAAAVLGSFLIARLSARRAAVAGLLLVAVASALRGAAHSTAVLFAMTF
                     GMGVGIAVIQPALPALVGQWFPARIGLATAVYANGLLVGETLPAAFTLPVVLPLVGGR
                     WEWALAVWSVPVFVTALLLRGPEAPSAAGPVPARRRWGPDWKAAATWRLGLLLGGASA
                     VYFGANAFIPEFLQATGRPQLIAGALAALNACQLPASAVMLVVASRMVGQWQPLVLAG
                     GLMLASLGAFLLLPAWSIVPSAGLLGFCSASLLILILALPPLLASRDDVPRLAAGMFA
                     IGYSLPFVLSLLGGAAWDVTGVPATAFIPVAAGSLAVLGVAVGFRRRA"
     gene            complement(2738..3457)
                     /locus_tag="VEW91_04555"
     CDS             complement(2738..3457)
                     /locus_tag="VEW91_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90887.1"
                     /translation="MSAAPFLTVEAVHAGYGRIPILHGVSLTVQAGEVVSIIGPNGAG
                     KSTALKVIVGLLTPWQGQVRFDGEIISGRRPDEILHRGLAFVPQGRIVFPQMTVLENL
                     EMGAYSERDRTRVDLALERVHRLFPVLFERRKQKAGTLSGGEQQMLTIGRALMIQPRL
                     LLLDEPSLGLSPKFLDIIFDKLAELKAAGMTMMIVEQNAVKALALANRGYVLELGRNR
                     FEGPGPALLADPEVKRLYLGG"
     gene            complement(3454..4176)
                     /locus_tag="VEW91_04560"
     CDS             complement(3454..4176)
                     /locus_tag="VEW91_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009487302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM90888.1"
                     /translation="MAEAAGTPVLELAGVTKRFGGVTAVDRCSIALQPGRIYGLIGPN
                     GSGKTTLFNCITGVEARDTGAIRLRGRRIDGLAPHRIARLGVGRTFQVIRVFPELTAL
                     ENLLVVSWVDRTTALARARDLLAFVRLADLADEPAGNLSYGQQKLVEFARVLMMDPFL
                     ILLDEPAAGVNRTLLNELLGEIRQLRDEGRTLLIVEHDMRIVMGLCEHVFVLDYGEKI
                     AEGPPDVIQRDERVIEAYFGRR"
     gene            complement(4230..5150)
                     /locus_tag="VEW91_04565"
     CDS             complement(4230..5150)
                     /locus_tag="VEW91_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90889.1"
                     /translation="MKRAIQVLLPVGLVVLAGYPVVASGYGIRFMLQLFMWVTLAQSW
                     NLISGLTGYVSFGHVAFFGTGAYATAILVAAHGWHWAAASLAGGGAAVVLALVIGYPC
                     LRLKGPYFAISMLGLNEVMRVAVSYFQGFTGGGLGLSLPTLYASVPIYYAMGLAAVTV
                     TAVVYGIITSRFGLRLMTIREDEVAAEAVGIDTARYKLYAFLLSAFWPGVVGGLLARD
                     QGYIDPVSVFPLTTTITMIVMALFGGKGTVWGPVLGATGLFVLQELVWARFLYLHQLL
                     FGAIIVVVVLGMPRGILGMLQQRYRLPRYV"
     gene            complement(5147..6019)
                     /locus_tag="VEW91_04570"
     CDS             complement(5147..6019)
                     /locus_tag="VEW91_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90890.1"
                     /translation="MTITPVMLGQVVVSGLLAGALYGMVALGLGLIFGVMRVINIAHG
                     TLLTLGAYTTFWWFHLLGVNPYLSLLVSMPLMFLLGVVLQRTLVARVVDAPELSSLLL
                     TFGISIALVNGMRLAFTSNLRSVEFLTGSIVVGEIALSKSRLIAFAFAVALTALASWV
                     IQRTKVGKAIRATSQSRDLAMFCGIDVARVRLYAFGIASALAAAGGSLVAVMVAIQPE
                     MGQGYTFKSFLVIVLGGAGNYPGALLGGLLLGLVEQLSSLFLTTQVSEAVAYVLLVLV
                     LLLRPQGLLGGRAA"
     gene            complement(6097..7272)
                     /locus_tag="VEW91_04575"
     CDS             complement(6097..7272)
                     /locus_tag="VEW91_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYM90891.1"
                     /translation="MRRVLAVGLLLALWTPAPLAMSAEGPIRIGASLSLSGTYASLGQ
                     NQRRGYELCAKDVNERGGVLGRKIEFVLYDDQSDPATGVRLYEKLITQDKVDVLLGPY
                     SSPITEAVANVNEKYMMPLVAPQASTTSIFRKGRKYIFMVTSPAEVYLEGLIDLAARQ
                     GLKTVALVNEDTLFPKATVDGTVDLAKKRGLRVVFVEAYPKGNTDFSAILTKVRAANP
                     DVFGAATYFDDAVAITRQMKELNVNPKMFGVTVGGDLPTFYQLLGKNAEFIYGATQWE
                     PVLPYPGIRRFVTDYYTEYPGADLSYHSAGGYGGCQILVEAIRRAGSLDREKLREALL
                     KLEMTTVYGAYKVDKDGFQIAHKMVTFQWQDGKKVIIWPDQLAAGKARFPTPSWSQR"
BASE COUNT         1260 a         2567 c         2522 g          947 t
ORIGIN      
        1 cacctcccgg gtcaccggct ggctgcccgc gggcccgttc ttccaacccc cgatgatagc
       61 ggagaacagc cccgcagttc cgccggcgtg cacgagcagc aggttttcag gcaggaactt
      121 cggtgatgct cctgcggcga acttctccgg catgccttcg gcacatcccc cggcgccgcg
      181 gacgatctcg gcccccggca gcgtcagcac acggacgatc tcttcccgca ggcgcgcctt
      241 cgaccagccc gcctcgcgga acacgcgccc gtgctcgggg gagatgatga ccagggcgtc
      301 gtagtcgcgg acgaacttgg gatgggcgac ggtgcgcagg caccaggcca gggaacggct
      361 gagcgattcg ggagtccgcg agagttggtc gaagacgccg cgcggcccct cgccggcaaa
      421 cacggtcacc cccgaggtgc cggggggcag gccgcgctcg accgacagcg gttcccacgg
      481 cgagcggtct tcgtcctccg cgaagcagaa ggtgtacttc ccgggattgc ccagggtggc
      541 ccgatccacc tctccgggcc gccccccgcc gacgttgcgg atcaccagct gcagggctcg
      601 cccgacggcc gcgttggcgc ggttcccctg tccgagcgcg ttcacgccgg agttcatccc
      661 gatggctttg gcgatgggac cgttcacgac gaccacgggg ccgacaaaat aggtcgtcgc
      721 caggacgcca tggatgttga acgcgtcggt gcacacggcc tccaccgcag cgaggacgac
      781 cggcaggtac tcggggcggc acccggccag cacggcgttg atggccacct tctccacggt
      841 gcacccggcg tagtcggggg gcacgaccgc cacgacctcg tccggagccc ggggggtgcc
      901 ctcgagcatc cggagcaccc gctccggcgt tggtggcacc accggcagcc cgtcgctcca
      961 gccgcgcgcg tggcaggcct cgatcgcgtc ctccgcctct ccaatagcga tgcgccggga
     1021 gcgcaggcgc ccggcgccct cgcgggcggc cagccgctcg gcgatcccgg gctccatcgt
     1081 ccgggacccg catcccggcc ggaacgccgg cagcccgtcc ccgagaccgc cgacgcgcgt
     1141 caggcgctcc caccggtcgc gcatccagcc ttccgtccgc tcgacgaccc gaccgccgac
     1201 cacgcgcagg agggtcggga ccgtctcgat ctgcaggccc acagaaacgt cgagccccgt
     1261 gtcgtcgaca acgggaaggc ccggagggaa ccccgggtcg tcctgggagt agatggtgag
     1321 cgccgccccg gcggcccgga gctgggtcag cacggggacg accaggcggc aggtggggca
     1381 gtcgcgcttg acgaccgcga caatcccctc ggggagcgcg atggcggcgg gcagatcgat
     1441 ccccccctct gaacacgggt tcggcgcgag cctcaccccg caccccccag tatagggtcg
     1501 gcgtctcggc cgggcaacgc tacgcgcgtc tccggaaccc cacggccacc ccgagcacgg
     1561 ccaggctccc ggccgcgacg gggataaacg ccgtcgctgg gacaccggtc acgtcccagg
     1621 ccgcgccgcc cagcagcgac agcacgaacg ggaggctgta gccgatcgcg aacatccccg
     1681 ccgcgaggcg cggcacatcg tcccgagacg ccagcagcgg gggaagcgcg aggatgagga
     1741 tgagcaggga ggcggagcag aagccgagga ggcccgcgct cggcactatg ctccatgccg
     1801 ggagcagcag gaacgctccg aggctggcca gcatgaggcc ccccgcgaga acgagcggct
     1861 gccactgtcc caccatccgg gacgcgacaa cgagcatcac cgcggaggcg ggcagctggc
     1921 atgcgttcag tgccgcgagc gcgccggcga tgagctgcgg ccggccggtc gcctgcagaa
     1981 actcggggat gaacgcgttg gctccgaagt acaccgccga ggccccgccc agcagaagtc
     2041 ccaaccgcca cgtcgccgcc gccttccagt ccggccccca gcgccggcgg gccggcacgg
     2101 ggccagcggc ggagggcgcc tccgggcccc ggagaagcag cgcggtcacg aacaccggga
     2161 cggaccacac cgcaagggcc cactcccacc gcccgccgac cagtggaaga accacgggga
     2221 gcgtgaacgc ggcggggagg gtctccccca ccaggagccc gttggcgtag acggccgtgg
     2281 cgaggccgat gcgcgcgggg aaccactgcc cgacgagggc ggggagcgcc ggctggatga
     2341 cggcgatgcc gacccccatg ccgaacgtca tggcgaagag cacggccgtg gagtgggcgg
     2401 caccgcgcag cgcggaggcg accgcgacga gcagaagacc ggcgacggcc gcgcggcgcg
     2461 cgctcaggcg ggcgatcaga aacgatccga gcactgcggc cgctcccagc accagcaccg
     2521 ggaggctcgt cagggccgcg accccggtct cattgaggcc aagagccgcg tggatccgcg
     2581 gcaacagcgg agggacggcg agcatcgtca ggcgcagatc ggcgcccagg agccacagga
     2641 gcacgaactg gaaccgcgcg ggagccacgt cttcctcaga tcacttgctc gggcatgacg
     2701 ctgtgcgttg atcgtagcag acccgggcgg ccacccccta accccccagg tagaggcgct
     2761 tcacttccgg atcggcgagg agggccggcc ccgggccctc gaagcggttc cggccgagct
     2821 cgaggacgta gccgcggttg gccagggcta gcgccttcac cgcgttctgc tccacgatca
     2881 tcatcgtcat tccggccgcc ttcaactcgg ccagcttgtc gaagatgatg tcgaggaact
     2941 tcggcgagag cccgagcgac ggctcatcga ggagcaggag ccgcggctgg atcatgaggg
     3001 cgcggccgat cgtgagcatc tgctgctcgc cgcccgagag cgtgcccgcc ttctgcttcc
     3061 ggcgctcgaa gagcaccggg aagagccggt ggacgcgctc gagcgccagg tccacgcggg
     3121 tgcggtcccg ctcgctgtag gcgcccatct cgaggttctc gagcacggtc atctgcggga
     3181 acacgatgcg gccctggggg acaaaggcga ggcctcggtg gaggatctcg tcggggcggc
     3241 ggccgctgat gatctcgccg tcgaaccgga cctgcccctg ccacggcgtg aggaggccga
     3301 cgatgacctt gagggccgtc gacttccccg cgccgttcgg gccgatgatc gagaccacct
     3361 cgccggcctg caccgtcagg ctgacgccgt ggaggatggg gatgcggccg tagccagcgt
     3421 gcaccgcctc gacggtcaag aacggggccg cgctcatcgc cgtccgaaat atgcctcgat
     3481 cacccgctca tcccgctgga tcacgtcggg cggcccctcg gcgatcttct ccccgtagtc
     3541 caggacgaag acgtgctcgc acagccccat gacgatccgc atgtcgtgct cgacgatcag
     3601 gagcgtgcgc ccctcgtcgc gcagctgacg gatctcgccg agcagctcgt tcaggagcgt
     3661 ccggttcacc cccgccgccg gctcgtcgag caggatcagg aacggatcca tcatcaggac
     3721 ccgcgcgaac tcgacgagct tctgctggcc gtaggacagg ttgccggcgg gctcgtccgc
     3781 cagatccgcg agccggacga aggccaggag gtcgcgtgcc cgggccagcg cagtcgtccg
     3841 gtcgacccac gagacgacga ggaggttctc cagcgccgtc agttccggga aaacccggat
     3901 gacctggaac gtccgtccga ccccgagccg ggcgatccgg tgcggggcga ggccgtcgat
     3961 ccggcgcccc cgcaggcgga tggctcccgt atcgcgcgcc tcgaccccgg tgatgcagtt
     4021 gaacaacgtc gtcttccccg agccgtttgg accgatcagc ccgtagatgc ggcccggctg
     4081 gagggcgatc gagcagcggt cgaccgccgt gacgcctccg aagcgcttgg tcacccccgc
     4141 cagctcgagg accggtgtcc ccgccgcctc agccatgtcg tcgggcgcgc ccggccgggc
     4201 gggcgatcgc ggcggggctc ggtccggcgt cagacatacc gcggcagccg gtaccgctgc
     4261 tgcagcattc ccaggatgcc gcgcggcatg cccaggacga ccacgacgat gatggcaccg
     4321 aagaggagct ggtggaggta gaggaagcgg gcccacacga gttcctggag cacaaagagg
     4381 ccggttgccc cgagcacggg gccccacacg gtccccttgc caccgaagag cgccatgacg
     4441 atcatggtga tcgtggtggt caaggggaag accgagaccg ggtcgatgta gccctgatcc
     4501 cgcgccaaga gcccgcccac cacgcccggc cagaaggcgg agaggaggaa cgcgtacagt
     4561 ttgtagcgcg cggtatcgat gcccacggcc tcggcggcga cctcgtcctc acgaatcgtc
     4621 atcagccgga gcccgaagcg tgaggtgatg atgccgtaga cgaccgccgt gaccgtcacc
     4681 gccgccagcc ccatcgcgta gtagatcggc acgctcgcgt acagggtggg gagcgagagc
     4741 ccgagcccac cgcccgtgaa cccctggaag taggagaccg cgacccgcat cacctcgttg
     4801 aggccgagca tcgagatcgc aaagtacggg cccttgaggc ggaggcacgg gtagccgatc
     4861 accagcgcca gcacgaccgc cgcccccccg cccgccagcg aggcggcggc ccagtgccag
     4921 ccgtgggcgg ccaccaggat cgcggtcgcg taggcgcccg tcccgaaaaa cgccacgtgg
     4981 ccgaacgaga cgtagccggt caggccggag atcaggttcc agctctgcgc cagggtcacc
     5041 cacatgaaca gctgcagcat gaatcggatg ccgtagccgg atgccacaac cgggtagccg
     5101 gccaggacga ccagcccgac ggggaggagc acctggatcg cccgcttcac gcggcccgcc
     5161 cccccaggag cccctgcggc cgcagcagga gcacgagcac cagcagcacg tacgccaccg
     5221 cttccgagac ctgggtcgtg aggaacaggg aactgagctg ctcgacgagg ccgaggagca
     5281 ggccgccgag cagcgctccc ggatagttcc cggcgccccc cagcacgatc accaggaatg
     5341 acttgaaggt gtacccctgc cccatctcgg gctggatcgc caccatcacg gcgaccaggc
     5401 ttccgccggc ggcggccagc gccgaggcga tcccgaaggc atagaggcgg acacgcgcca
     5461 cgtcgatccc gcagaacatg gccaggtcgc ggctctgcga ggtggcgcgg atcgccttcc
     5521 cgaccttcgt cctctggatg acccaggagg ccagcgcggt gagggccacg gcgaaggcga
     5581 aggcgatcag gcgggacttc gatagcgcga tctccccgac gacgatggag ccggtgagga
     5641 actcgaccga gcggaggttg gaggtaaacg ccagccgcat cccgttgacg agggcgatcg
     5701 agatcccaaa ggtcaggagc agtgacgaca gctcgggggc gtccaccaca cgggccacca
     5761 gcgtccgctg gaggacgaca cccaggagga acatgagcgg catcgagacg agcagggaga
     5821 gatacgggtt cacgccgagg agatggaacc accagaacgt cgtgtacgcc cccagcgtca
     5881 ggagggtgcc gtgcgcgatg ttgatgacgc gcatgacgcc aaagatcagg ccgagtccta
     5941 gggcgaccat gccgtagagc gcaccggcca ggaggccgga gaccacgacc tggccgagca
     6001 tcaccggcgt gatcgtcacg gtcccgggac caggccggcg gccgggtgag agggtcgcgc
     6061 ggggtcccct ctcactgtga ggcgcgggtc acccagttag cgctggctcc aggacggtgt
     6121 cggaaagcgc gccttgccgg ccgcgagctg gtccggccag atgatgacct tctttccgtc
     6181 ctgccactgg aacgtgacca tcttgtgggc gatctggaac ccgtccttat cgaccttgta
     6241 ggcgccgtac acggtcgtca tctccagctt gaggagggcc tcccggagct tctcccggtc
     6301 cagagagccc gcccgcctga tcgcctcaac gaggatctgg cagcccccgt agccgcccgc
     6361 cgagtggtag gagaggtcgg cgccgggata ctccgtgtag tagtccgtaa cgaacctccg
     6421 gatgccgggg tacggcagta ccggctccca ctgtgtcgcg ccgtagatga actcggcgtt
     6481 cttcccgagc aactggtaga acgtcggcag atccccgccc accgtgacgc cgaacatctt
     6541 ggggttgacg ttcagttcct tcatctgccg ggtgatcgcc acggcgtcgt cgaagtacgt
     6601 cgcggccccg aagacatccg ggttcgccgc ccgcaccttg gtcaggatgg cggagaagtc
     6661 ggtgttcccc ttcgggtagg cctcaacgaa cacgacccgg agtccccgtt tcttggcgag
     6721 gtcgaccgtc ccatcgaccg tcgccttggg gaacagcgtg tcctcgttga cgagggccac
     6781 cgtcttgaga ccctgacggg cggccaagtc gatcaggccc tcgaggtaca cctcggccgg
     6841 cgacgtcacc atgaagatgt acttccggcc cttcctgaag atggacgtgg tcgacgcctg
     6901 cggggcgacc agcggcatca tgtacttctc attgacgttg gccaccgcct cggtgatcgg
     6961 ggaggagtag ggcccgagga gcacatccac cttgtcctgg gtgatcagct tctcgtagag
     7021 gcggacaccg gtcgccggat ccgactgatc gtcgtagagc acgaactcga tcttgcggcc
     7081 gaggacgccg ccccgttcgt tcacgtcctt ggcgcagagc tcgtagccgc ggcgctggtt
     7141 ctggccgagg gaggcgtaag tgcccgagag cgagagcgag gcgccgatcc tgatcggccc
     7201 ctcagcggac atcgccagcg gagcgggggt ccacagcgcc aggagcagcc cgaccgcgag
     7261 gacacgtctc acgatacgat cacctccagc ggccta
//
LOCUS       DASQGH010000239         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1056, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000239 DASQGH010000000
VERSION     DASQGH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VEW91_04580"
     CDS             <1..932
                     /locus_tag="VEW91_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90892.1"
                     /translation="RVFQWLEAHHGEAGQVRGLDLENRETTVQVPALDLGQPVDAYAY
                     TSEKIFYLRTNLVGSFPHAAYEMVRSRLENVPDVSAAFIQARAAELDALTDDVLRDAG
                     PPGDYFHTLNREQVRTVLARLIAFDNGRAMLDITRILPPDRVVANPMDPTRLASVILL
                     KRDFSDRMVLESLSLGKFIGRLLRGETPERKKETAYNAYRAVDDEVERRFIDRLEAEA
                     AAEGATTREALYAALTRHQDVPETLAEEVELFRMMHQACRCYDLNTILATDPTVNGLK
                     EAVNLTMGLIAHALALGQGSVHLTLDDYRSAVS"
     gene            932..2308
                     /locus_tag="VEW91_04585"
     CDS             932..2308
                     /locus_tag="VEW91_04585"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYM90893.1"
                     /translation="MRAIAYAEAHRARFLDALKEFVRIPSISTLADHHPDLGRAADWV
                     AQRLIDAGLSARVMPSDSHPAVYAEWTGAPGRPTLLCYAHYDVQPPDPLDLWESPPFE
                     PVQRGEDLYARGVSDDKGQLLIQIFAVESLLKAGDGLPVNVKFFIEGEEEIGSPRMVP
                     FVKKHRRTLRADAALVCDTSFFAPERPALVTGLRGLVYTEVELRGATRDLHSGQFGGA
                     APNPIEGLARIIAGLKDRRNRVTIPRYYASVRPPSEVEQRSWAALGFREDAYLQDLGV
                     AEAPGEAGYSVLERRWARPTLEVHGITGGFTGPGMKTVIPARAAAKISMRLVADQRPQ
                     AVLWAFTRKVERLTPPGLRAEVRTLAAGGPVVLSPDLPALRSAARALEETFHRPPVFM
                     RTGGSVPLVAEFLHTLHIPPVLMGFSLPDDRAHSPNEKFHLPNFYRGIEAVIRFIQDL
                     GNGGSVGS"
BASE COUNT          357 a          800 c          815 g          341 t
ORIGIN      
        1 accgggtgtt tcaatggttg gaggcgcacc acggcgaggc cgggcaggta cgcggtctgg
       61 atctcgagaa ccgcgagacg accgtccagg tccctgctct cgacctgggg cagccggtgg
      121 atgcctacgc gtacacgagc gagaagatct tctacctcag gacaaacctc gtcgggagct
      181 tcccgcatgc ggcctacgag atggtgcggt cccggttgga gaatgttccg gacgtgtccg
      241 cggcgttcat ccaggcccgc gccgccgagc tcgacgcgct gaccgatgac gtgctccggg
      301 acgcggggcc gcccggcgat tacttccaca ccctgaaccg ggagcaggtg cgcacggtgc
      361 tcgcccgcct gatcgcgttc gacaacgggc gggcgatgct ggacatcacg cgcatcctgc
      421 ctcccgaccg cgtggtcgcg aaccccatgg accccacgcg cctggcgagc gtgatcttgc
      481 tgaagcgcga tttcagcgac cgcatggtgc tggagtcgct gtcgctgggc aagttcatcg
      541 ggaggctgct ccggggcgag acgccggagc gcaagaagga gaccgcctac aacgcctacc
      601 gggcggtcga cgacgaggtg gaacgccggt tcatcgaccg gctcgaggcg gaggccgccg
      661 cggagggagc caccacccga gaggcgctct acgccgcact gacgcgtcat caggacgtgc
      721 cggagaccct ggccgaggag gtggaattgt tccggatgat gcaccaggcc tgccggtgct
      781 acgatctcaa caccatcctc gccaccgatc caacggtgaa cggcctcaag gaagccgtca
      841 acctgacgat ggggctgatc gcgcacgcgc tcgccctcgg gcaggggagc gtgcacctga
      901 cgctcgacga ctaccggagc gccgtcagct gatgcgggcg atcgcgtacg ccgaggccca
      961 ccgcgcccgg ttcctcgacg cgctcaagga gttcgtgcgg atcccgagca tcagcacgct
     1021 ggccgaccac caccccgatc tcgggcgcgc ggccgactgg gtggcgcagc gcctcatcga
     1081 cgccggcctg agcgcccggg tgatgccgag cgactcgcat ccggcggtct acgccgagtg
     1141 gacgggcgcg cccgggcggc cgaccctcct ctgctacgcg cactatgacg tgcagccccc
     1201 cgatcccctc gacctgtggg agtcgccgcc gttcgagccg gtccagcgcg gcgaggacct
     1261 ctacgcccgc ggcgtctcgg acgacaaggg gcaactcctc atccagatct ttgccgtgga
     1321 atcgctgctc aaagccggag acggcctccc ggtcaacgtc aagttcttca tcgagggcga
     1381 ggaagagatc ggcagcccgc ggatggtccc cttcgtgaag aagcaccggc ggaccctgcg
     1441 tgcggatgcg gcgctggtct gcgatacgtc cttctttgcg cccgagcgcc ccgccctcgt
     1501 gaccggcctg agggggctcg tctacaccga ggtcgagctc cggggggcca cccgggatct
     1561 gcactcgggc cagttcgggg gcgctgcgcc caatccgatc gaaggcctcg cccgcatcat
     1621 tgcggggctc aaggaccgca ggaaccgcgt gacgatcccc cggtactacg cgtccgtcag
     1681 gccgccgagc gaggtcgaac agcggtcgtg ggcggcgttg gggttccgcg aggacgccta
     1741 cctccaggac ctgggggtgg cggaggcccc cggggaggcg gggtacagcg tcctcgagcg
     1801 ccggtgggcg cgccccacgc tcgaggtcca cgggatcacc ggcgggttta cggggccagg
     1861 gatgaagacg gtcatcccgg cgcgcgcggc ggccaagatc agcatgcgcc tggtggcgga
     1921 ccagcgcccg caggcggtcc tctgggcgtt cacccggaag gtcgagcgcc tgaccccgcc
     1981 cggactccgg gccgaggtgc gcaccctggc cgcggggggg cccgtcgtcc tctcgcccga
     2041 tctgccggcg ctgcgcagcg cggcgcgggc gctcgaggag acgtttcacc gccccccggt
     2101 gttcatgcgg acgggcggca gcgtgccgtt ggtggcggag ttcttgcaca cgctccacat
     2161 cccaccggtg ctcatgggct ttagcctgcc ggacgatcgc gcacactcgc ccaacgagaa
     2221 gttccacctg ccgaacttct accgggggat cgaggccgtc atccggttca tccaggacct
     2281 tgggaatggg gggtcggtgg ggtcgtagat gcg
//
LOCUS       DASQGH010000240         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3932, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000240 DASQGH010000000
VERSION     DASQGH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VEW91_04590"
     CDS             <1..211
                     /locus_tag="VEW91_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90894.1"
                     /translation="SPERLDLRDAHVRLARDRGARFEIGTDAHRTDTLQTMEYGIGTA
                     RRGWVEAPSVINTWPLQQLLDVLGD"
     gene            172..786
                     /locus_tag="VEW91_04595"
     CDS             172..786
                     /locus_tag="VEW91_04595"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYM90895.1"
                     /translation="MAAPATARCSRRLARLPRAFYARPTVAVARALLGCYLVHESPEG
                     IAVGRLVEVEAYCGPRDPASHAYRRTPRSEVMWGAPGTAYVYLSYGVHACMNVVTEAP
                     GRPGAVLLRALEPVAGVGLMQQRRGTRALRALASGPGRLTRAMGIALAHNRADLVTGP
                     LYLARRDRRPGPIAATRRIGISDATDRPWRFAVRGSPFLSRRVR"
     gene            817..1737
                     /locus_tag="VEW91_04600"
     CDS             817..1737
                     /locus_tag="VEW91_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90896.1"
                     /translation="MRAVRILMVFVDGVGLGDPDPAINPLVRAATPTLRRLLDQPLAG
                     RAPVSTAEAVLVPLDAQLGVPGLPQSGTGQAAILTGHNAPAVAGRHVGAYPTLALRAL
                     LEERGLFTQLRRRGIDPALANAYTPEYFTAVAERRLRHAAITLNALQAGVRLRDLDDV
                     RAGEAVYHDLTNARLRGWGHAVPELSPEDAGRNLAGLAARHPFTLFEFFQTDLAGHRR
                     LPDPVGVVERFDRFLGAILDGTDMGATLMIVTSDHGNLEDERTHGHTLNPVPALLVGA
                     GRERVGERLHDLTDVTPACVDLLGNGVEPS"
     gene            1734..>2892
                     /locus_tag="VEW91_04605"
     CDS             1734..>2892
                     /locus_tag="VEW91_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HYM90897.1"
                     /translation="MTPRTLRVLELSTILERLAALCASPLGGAAARALGPSIALEEVR
                     RRHQETSEARELAETAGGLPVRGIRDVRDPVHRASIGGVLPVQQLLDIRDTAAVARAL
                     KGFLTARREAVPILAGLAASFEVFPGLEEAIGNAVGSDGEILDGASRDLARIRRDRRT
                     AESRLRDRLDGVLRDAAIVRMLREPLITIRGDRYTIPVRAEFRHQFPGIVHDQSSSGV
                     TVFMEPLAVVPLGNRVRELAAAERDEVARILAALSGEVGAVAEEFSRTLEVLAGIDLI
                     AAKAHLSIEMRGSSPGLNSEGVVELRSARHPLLSGTVVPIDVRLGGRCRTLIITGPNT
                     GGKTVTLKTIGLLTLMAQAGLHIPAAPESEVAVFPQLYADIGDEQSIEQNLS"
BASE COUNT          384 a         1020 c         1074 g          414 t
ORIGIN      
        1 atctcccgag cggttggacc ttcgggatgc gcacgtccgc ctggcccgcg accggggagc
       61 ccgcttcgag atcggcaccg atgcccaccg cacggatacc ctccagacca tggaatacgg
      121 tatcgggacc gcccggcgag gatgggtcga agcccccagc gtgatcaaca cgtggccgct
      181 ccagcaactg ctcgatgttc tcggcgacta gcgcggctcc cccgggcgtt ctacgcgcgg
      241 ccgacggtgg ccgtggcgcg cgccctcctc ggctgctatc tcgtgcacga gtcgccggag
      301 gggatcgccg tgggccggct ggtggaggtc gaagcgtact gcggtccgcg ggatcctgcc
      361 agccacgcct accggcggac cccccggagc gaggtgatgt ggggggcgcc cggaaccgcg
      421 tatgtctacc tgagttacgg cgtgcacgcc tgcatgaacg tggtcaccga ggcgccgggg
      481 cggccggggg cggtgctgct ccgggccctc gagcccgtgg ccggggtcgg gttgatgcag
      541 cagcggagag gcaccagagc cctccgggct ctggcgagtg ggccgggccg gctgacccgg
      601 gcgatgggga tcgcgctcgc gcacaaccgc gcggatctgg tgaccggccc cctgtacctc
      661 gcgcgccgcg atcgccgccc gggaccgatc gccgccacgc gccggatcgg gatctcggat
      721 gcgaccgacc gcccgtggcg gtttgcggtg cgggggtcgc cgttcctgtc gcgtcgggtt
      781 cgttgagccc gatccgggat cccggtttgt ggtatcatgc gggcggtgcg gatcctgatg
      841 gtatttgtcg acggcgtggg gttgggggac cccgatccgg ccatcaaccc gctggtgcgg
      901 gcggccacgc cgacccttcg ccggctcctg gaccagccgc tcgcggggcg ggccccggtg
      961 tccaccgcgg aggccgtgct cgtcccgctc gacgcgcaac tcggggtccc gggcctgccc
     1021 cagagcggga ccgggcaggc ggcgatcctc acggggcaca acgcccccgc cgtggccggg
     1081 cgccacgtgg gcgcgtatcc cacgctggcg ctcagggcgc tgctcgagga gcgagggctg
     1141 ttcacccaac tgcgccggcg cgggatcgac ccggccctgg cgaacgccta cacccccgag
     1201 tatttcacgg cggtcgccga gcgccgcctg cggcacgccg cgatcaccct caacgcgctt
     1261 caggccggcg ttcggctgcg cgacctggac gacgtgcgtg ccggggaggc ggtgtaccat
     1321 gacctcacca acgcgcggct gcgcgggtgg ggacacgccg tgccggagct ctcccccgag
     1381 gacgccgggc gcaacctggc cgggctcgcc gcccggcatc cgtttaccct gttcgagttc
     1441 tttcagaccg atctggccgg gcaccgccgc ctgcccgatc cggtcggcgt cgttgagcgg
     1501 ttcgaccggt ttctcggggc catcctcgac gggaccgaca tgggtgccac cctgatgatt
     1561 gtgacgagcg accacggaaa cctggaggac gagcggacgc acgggcacac gctcaacccc
     1621 gtgccggcgc tgctcgtggg ggccgggcgg gagcgggtgg gggagcgact ccacgacctg
     1681 accgacgtga ctccggcgtg cgtcgacctc ctcggcaacg gggtggagcc ctcgtgaccc
     1741 cgcgcaccct ccgggtcctg gagctgtcga cgatcctgga acggctggcg gcattgtgcg
     1801 cgtcgccgct cgggggtgcc gcggcccgag ccctcgggcc gtccatcgcg ctggaggagg
     1861 tgcggcggcg gcaccaggag acgtcagaag cacgggagct cgcggagacc gcaggcggcc
     1921 tccccgtgcg ggggatccgc gacgtccggg atcctgtgca ccgcgcgtcg atcggaggcg
     1981 tcctccccgt gcagcagctg ctcgacatcc gggacacggc ggcggtggcc cgggcgctca
     2041 agggattcct gaccgcccgg cgcgaggcgg tgccgatcct cgccgggctc gccgcttcgt
     2101 tcgaggtgtt tcccggtctc gaagaggcga tcgggaacgc ggtggggtcg gacggagaga
     2161 tcctcgatgg ggccagccgg gacctcgccc ggatccggcg ggaccggcgc accgccgagt
     2221 cccggttgcg cgaccgcttg gacggggtgc tgcgcgatgc ggcgatcgtc cggatgctgc
     2281 gggagcccct gatcacgatc cgcggcgacc ggtacacgat cccggtcagg gcggagttcc
     2341 gccaccagtt cccggggatc gtccacgacc agtcatcgag cggcgtgacg gtgttcatgg
     2401 agcccctggc ggtcgtcccc ctcggcaacc gcgtccgcga gctggccgcc gccgagcgcg
     2461 acgaagtcgc ccggatcctc gccgcgctga gtggggaggt gggggcggtt gcggaggaat
     2521 tctcgaggac cctcgaggtg ctggccggga tcgatctgat cgcggcgaag gcccacctca
     2581 gcatcgagat gcgagggtcc tcccccggac tcaacagcga gggggtggtg gagcttcgta
     2641 gcgcccgcca cccgctgctg tccgggaccg tcgtcccgat cgacgtcagg ctcggcggcc
     2701 ggtgtcgcac gctgatcatc acgggcccca acaccggggg aaaaacggtg accctgaaga
     2761 cgatcgggct cctcaccctc atggcgcagg ccgggctgca tatcccggcc gcacccgaga
     2821 gcgaggtggc ggtgttcccc cagctctacg ccgacatcgg cgacgaacag agcatcgagc
     2881 agaacctctc ga
//
LOCUS       DASQGH010000241         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20823, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000241 DASQGH010000000
VERSION     DASQGH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20823"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VEW91_04610"
     CDS             complement(<1..700)
                     /locus_tag="VEW91_04610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYM90898.1"
                     /translation="MGKIGIALPNFPGVTPPLIPEFARRIEAGGCDAVWCLDRLVYEN
                     PDPLTALAAAAAVTSRVRIGTSILLAPLHSPVVLAKQLATLDLVSNGRLLVGIGVGWR
                     PDDFAGAAVPYAGRGGRTAEMIRIMKQVWSGQPVRHQGRHFTIECGPVGPAPVQRPHP
                     PVWMGGIQEVALRRVARIADGFIVTGGAGPEGFAATWQRMRQMVADAGRAPDQFPNAC
                     LAYFNVGNDREQAKA"
     gene            complement(752..2095)
                     /locus_tag="VEW91_04615"
     CDS             complement(752..2095)
                     /locus_tag="VEW91_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM90899.1"
                     /translation="MTMLIWFQGQVQTAIARRGGPLLLVRGEGSRVWDDRGRSYLDAR
                     SGVWAVTIGYGRADVVEEVSSQLARLSFAPLTDIASPAALALAAGLGERLPDDLTTVA
                     LVPTGSEAVDTALKFARAYHSAGGDRRRRIVISREYSAHGSTYAGSSLSDPDRGLLRG
                     IGGRLSGIRFVPAPYRYRCRYCAEGPACTLACVRVVEDAIAAAGSARVAAVFAEPVPG
                     PGGVLVPPDEYWPRLRDLCDRHGVLLVADEVVTGFGRTGRWFACAHWNVVPDLMILGK
                     GMTGGYQTLGAVAMRRHVSLRLERRLIPHGFTYSGHPAACAAALACLRIIHAEGLVAR
                     AAARGARLLDGLRRALAPCPVVGEVRGLGLMAAVELVEDRASRTPLRLRARDLDRLEQ
                     DLRDREVLAFVDNPVILAPPLVVTDEEIDHLVEATAAAIRALGERVSGAAPRARA"
     gene            complement(2157..>2932)
                     /locus_tag="VEW91_04620"
     CDS             complement(2157..>2932)
                     /locus_tag="VEW91_04620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYM90900.1"
                     /translation="VAHPQRRVWNFLRPLISRYDGAVFSLPRFAQRLAIPQYLVYPSI
                     DPLSDKNRDLTEEEIDAIMARLGVPRDKPILLQVSRFDRFKDPLGVIHAYRMVKRHDD
                     CRLVLAGGGAADDPEGPKVLAEVEAAAAGDPDVHVLVLPDDAHLEINALQRAATIVLQ
                     KSTREGFGLTVAEAMWKGKPVIGGFAGGITVQIVYGTTGYTVNSVEGAAFRARYLLNN
                     PELARRMGENGREYVRRNFLITRHLTDLVTLLIIHTASA"
BASE COUNT          453 a         1051 c         1032 g          396 t
ORIGIN      
        1 gggccttggc ctgttcccga tcgttgccca cattaaagta ggccaggcac gcattgggga
       61 actggtctgg agcgcgcccg gcgtcggcca ccatctgccg catccgctgc cacgtggcgg
      121 cgaacccctc cggcccggca cccccggtca cgatgaaccc gtcagcgatc ctggcgaccc
      181 gcctgagcgc cacctcctga attcccccca tccacaccgg cgggtgggga cgctggaccg
      241 gggcgggccc gaccggtccg cactcgatcg tgaagtgcct gccctggtgg cggacgggct
      301 ggccgctcca cacctgcttc atgatccgga tcatctccgc cgtccgcccg ccccgaccgg
      361 cataaggcac cgcggcaccg gcgaagtcgt ccgggcgcca gccgaccccg atcccaacaa
      421 gcaggcgccc gttcgacacg aggtcgagcg tcgcgagctg cttcgcgagg acgaccgggg
      481 agtgcagcgg agcgagcagg atcgaggtcc cgatccggac gcgggacgtc accgcggcgg
      541 cggccgccag cgcggtcagc ggatcggggt tctcgtagac gaggcggtcg aggcaccaga
      601 cggcatcgca cccaccggcc tcgatgcggc gggcaaactc cgggatgagc gggggggtga
      661 cccctggaaa attcggtagc gcgatcccga tcttgcccat ggcggcacca agcttggatt
      721 acgcgaagat cgagagcggt tcctgcgctg ctcaggcgcg cgcgcgaggc gccgcgccgg
      781 agacgcgctc cccgagcgcc cggatggccg cggccgtggc ctcaacgagg tggtcgatct
      841 cctcgtccgt gaccacgagc gggggggcga ggatcaccgg attgtccacg aacgcgagca
      901 cctcgcggtc acgcaggtcc tgctcgaggc ggtcgaggtc ccgggcccgc agccgcagcg
      961 gcgtccggga cgcgcggtcc tcgaccagct cgaccgcggc catcaggccg aggccgcgaa
     1021 cctcgccgac gaccgggcac ggggcgagcg cgcgcctgag gccgtcgagc agccgcgctc
     1081 cccgggcggc tgcgcgggcg acgaggccct cggcatggat gatccgaagg cacgccaggg
     1141 cggcagcgca cgccgccggg tggccgctgt aggtgaagcc gtggggaatg agacggcgct
     1201 cgaggcgcag ggagacgtgc cggcgcatcg ctaccgcgcc gagggtctgg tatccgccgg
     1261 tcatcccctt cccgaggatc atgaggtccg gcaccacgtt ccagtgggcg cacgcaaacc
     1321 atcggccggt ccgcccgaac ccggtgacca cctcgtccgc gacgagcagg acgccgtggc
     1381 ggtcgcagag gtcgcgtagg cgcggccagt attcgtccgg cggcacgagc acgccccccg
     1441 gcccggggac gggctccgcg aacaccgcgg cgacccgggc cgatcccgca gccgcgatcg
     1501 cgtcctccac gacccgaaca caggcgagcg tgcaggccgg accttccgcg cagtaccggc
     1561 accggtaccg gtagggggcc ggaacaaacc ggattccaga gagccggccg ccgatcccgc
     1621 gcaggagccc ccggtcggga tcgctcaacg acgatcccgc gtacgtgctg ccgtgggccg
     1681 agtactcccg gctgatcacg atgcgccggc ggcggtcgcc cccggccgag tggtaggccc
     1741 gcgcgaactt gagcgcggtg tcgacggcct cggagccggt cgggacgagg gccaccgtcg
     1801 tcaggtcgtc cgggaggcgt tcgcccagcc ctgccgcgag ggccaacgcc gcgggggacg
     1861 cgatgtcggt gagaggggcg aacgagagcc gcgcgagctg gctactcacc tcctcgacga
     1921 cgtccgcgcg gccgtagccg atcgtgaccg cccacacccc cgagcgggcg tccaggtagg
     1981 accggccccg gtcgtcccag acccggctgc cttctccccg caccaggaga agcgggccgc
     2041 ccctgcgagc gatcgccgtc tggacctgtc cctgaaacca gatcagcatg gtcatccgcg
     2101 ttatccctcc caagcggcgg tcgcgacgtg attctccgcc ccgggaggat ttcccgtcag
     2161 gcgctcgcgg tgtggatgat cagcagcgtc accaggtcgg tgaggtggcg ggtgatgaga
     2221 aagttgcggc ggacgtactc acgcccgttc tcgcccatcc gcctcgcaag ctcaggattg
     2281 ttcagcaggt agcgcgcccg gaacgcggcc ccttcgaccg agttcaccgt gtacccggtg
     2341 gtcccgtaga cgatctggac cgtgatcccg ccggcaaacc cgccgatcac gggcttgccc
     2401 ttccacatcg cctccgcaac ggtcaagccg aatccctcgc gcgtcgattt ttgtagcacg
     2461 atcgtggcag cgcgctgcag ggcgttgatt tcgaggtgcg cgtcgtcggg gaggacgagc
     2521 acatggacgt ctggatcgcc cgccgccgct gcttccacct cggccaggac tttcgggccc
     2581 tccggatcat cggcggctcc gccgccggcc aggaccaacc ggcagtcgtc gtgtcgcttc
     2641 accatccggt aggcgtgaat gacccccagc ggatccttga accggtcgaa ccgggagacc
     2701 tgcaggagga tgggcttgtc ccgcgggacg ccaagccgcg ccatgatcgc gtcgatctcc
     2761 tcctcggtca gatcgcggtt cttgtcgctg agcggatcga tgctcgggta gaccaggtat
     2821 tgcggaatcg ccaggcgctg ggcgaagcgc gggagcgaga acacggcgcc gtcgtaccgc
     2881 gagatgagcg gccggaggaa gttccacacc cggcgctgcg ggtgggccac gt
//
LOCUS       DASQGH010000242         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_37993, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000242 DASQGH010000000
VERSION     DASQGH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37993"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VEW91_04625"
     CDS             complement(<1..575)
                     /locus_tag="VEW91_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HYM90901.1"
                     /translation="MGPARSPDPPPPPHREQRHRYHRRTWRQFFQVMGPGIVSGAADN
                     DPAGVVTYIQVGATTGTGLLWLMFLSTPMLYYLEEMSTRLGVVTKRGIGHILRTHYGS
                     RAAAVVLVPVVLSNVITIGADLSGTSAALQLLTGIAWEWWTVPLAAVMASTLIFAGYR
                     TISRFLLLLTPLFALYIITGVIVQPPWNAVLR"
     gene            complement(575..1684)
                     /locus_tag="VEW91_04630"
     CDS             complement(575..1684)
                     /locus_tag="VEW91_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYM90902.1"
                     /translation="MRIARIDVFGYDLHYVHGSYVMSGGRVVTALPSTVVRLTTDDGL
                     VGWGEVCPLGPAYLPAFAEGARTALRELAPALLGLDPQNLGLIHDRMDAALRGHGYAK
                     SPVDMACWDLLGKAAGCAVATLLGGLRQASYPLYIAVPLGSAEEMAEYVRARRAEGIH
                     RFQLKIGGDPYADRDRVERVLEATGEADIVIADANGGWSLQDAVVASRLLAPLPRVYL
                     EQPCPTLEECLHVRRHTTLPMILDEVITDVPSFLRAVHAGAMEAINLKIGRVGGLAKA
                     RLIRDLAEALGLRLTIEDMWGGDIVTAAISQLAGSTRPGMLFTVSFMNDWVREHIAGH
                     QPRSQGGVGFTSSAPGLGIEVDAKSLGHPLISAAE"
     gene            1835..2812
                     /locus_tag="VEW91_04635"
     CDS             1835..2812
                     /locus_tag="VEW91_04635"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HYM90903.1"
                     /translation="MLRTRICDLLRIDHPVVLGGMASHSNPALVAAVTRGGGLGILGV
                     TVMAPEEQRAAVRRIRELTDGPFGLNHLLAFATEDRFAVTLEARPRVVSTAWAWAEQD
                     LHPYFDRAHEAGALVMHMVSTVPEAVRAVGAGADIIVAQGTEGGGHVGWISTLPLVPQ
                     VVRAVAPVPVLAAGGIADGAGLAAALALGAEGVLLGTRFLATPEAPIPAGYKEAILAS
                     DGHNTLLTEVADIAFGRVWPGAMSRVIRNQFVERWVGREWEVRQRHHEIARGLRAARE
                     RDDAGESNLQMGQAAGLITEVAPAADIVREIVAQAERIIRSRLPSLLAD"
     gene            complement(2829..3848)
                     /locus_tag="VEW91_04640"
     CDS             complement(2829..3848)
                     /locus_tag="VEW91_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90904.1"
                     /translation="MRTAGPRFGVVYEHPTWFAPLFAELDRRRIGYDRVDLGTHTFDP
                     DRRAVPWSLVLNRLSPSADLRGHGQAIAYGREFLRYLEAAGTDVVNGSAAFTLEISKV
                     AQVLLLRQLGLRAPRTLVINHPSQAPAPAAGLDFPVIVKPNIGGSGAGMQRFESPEAL
                     GRAAEAGALALGVDRIGLVQEFLPARGGHIVRVEVLDGRFLYAIKVYPNPEAGFNLCP
                     ADICHPDAGAEPAARATSADLCPVDLPKVALRVEGYTPPEAVTQDVVAITRAARIDLG
                     GVEYLVNDRDGEVYYYDINALSNFVTDAPRIVGFDPYAVLVDYLVARGRLRRPIPSVS
                     SAARR"
     gene            complement(3991..>4179)
                     /locus_tag="VEW91_04645"
     CDS             complement(3991..>4179)
                     /locus_tag="VEW91_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HYM90905.1"
                     /translation="QVTDLYTPERLIGRTVVAAMNLGPRRIAGFTSEVLVLGVPDDTG
                     RVVLLSTEREVPAGGRVF"
BASE COUNT          634 a         1415 c         1531 g          599 t
ORIGIN      
        1 cgcagaacgg cgttccaggg gggctggacg atgaccccgg taatgatgta cagggcaaag
       61 agcggggtca gcagcagcag gaaccggctg atcgtccggt agcccgcgaa gatgagggtg
      121 gaggccatca cggcggccag ggggacggtc caccattccc acgcgatccc ggtgagcagc
      181 tgcagcgcgg cgctggtgcc ggaaagatcc gcgccgatcg tgatgacgtt cgagagcacg
      241 accggaacga ggacgacggc cgccgcgcgg ctgccgtagt gggtccggag gatgtggccg
      301 atcccccgct tggtcaccac gccgagccgg gttgacatct cctccaggta gtagagcatc
      361 ggggtcgaca ggaacatcaa ccacaacagg cccgtccccg tcgtggcgcc cacctggatg
      421 taggtcacga cgccggcggg gtcgttgtcc gcggccccgg acacgatccc gggacccatg
      481 acctgaaaga actgccgcca cgtgcgccgg tgatacctgt ggcgctgctc gcggtgcggt
      541 ggggggggcg ggtccgggct ccgcgccggc cccattactc ggcggcgctg atgagcgggt
      601 ggccgaggct cttcgcgtct acctcgatgc ccaggccggg ggcgctggac gtgaacccca
      661 ctcccccctg cgatcgaggc tggtggccgg cgatatgttc gcgcacccag tcgttcatga
      721 acgacacggt gaagagcatc ccgggccggg tgctgccggc gagctggctg atcgccgccg
      781 tgacgatgtc gcctccccac atgtcctcga tcgtcagccg gaggccgagc gcctcggcca
      841 ggtcgcgaat cagcctcgcc ttggcgagcc ctccgacccg gccgatcttc aggttgatcg
      901 cttccatggc gccggcgtgg acggcccgga gaaacgacgg cacatcggtg atgacctcgt
      961 cgaggatcat cggcagggtt gtgtgccgcc gcacgtggag gcactcctcc agggtgggac
     1021 agggctgctc gaggtacacg cggggcaggg gggcgaggag gcgcgaggcg accacggcgt
     1081 cctggagcga ccatccaccg ttggcgtcgg cgatcacgat gtccgcctcc ccggtcgcct
     1141 cgaggacgcg ctccactcga tcccggtcgg cgtacggatc cccgccgatc ttcagctgga
     1201 agcgatggat cccctccgcg cgtcgggcgc gcacgtactc ggccatctcc tcggccgagc
     1261 cgaggggcac cgcgatgtac aggggatagc tggcctggcg caggccgccg agcagggtcg
     1321 ccaccgcgca ccccgccgct ttaccgagca ggtcccagca cgccatgtcg accgggcttt
     1381 tcgcgtagcc gtggccgcgc agcgcggcgt ccatccggtc gtggatgagc ccgaggtttt
     1441 ggggatccag gcccagcagc gccggtgcca gctcccgcag cgccgtcctg gctccctcgg
     1501 caaacgccgg caggtaggcg ggcccgagcg ggcacacctc cccccagccg accagcccgt
     1561 cgtcggtggt gagccggacc accgtgctgg ggagcgcggt gaccacgcgt ccccccgaca
     1621 tgacgtagct gccgtggacg tagtgcaggt cgtacccgaa gacgtcgatc cgtgcgatcc
     1681 gcatcgctcc ctctcatttc gggagcggtg ccccgactcc cgccgcgtcc gctgcgcccg
     1741 gcgcgggaag ggacgcgctc tcttgttcgg gaaggagtgt gggcccggcc ggttaccagc
     1801 cgccggcgga cggcggatct cgaggaggag ccatatgctg cgcacccgga tctgcgacct
     1861 cttgcgaatc gaccatccgg tggtgctcgg cgggatggcg tcgcactcga accccgcgct
     1921 cgtcgccgcg gtcacccgag gcggcggcct ggggatcctg ggcgtgacgg tcatggctcc
     1981 cgaggagcag cgggcggccg tccgccggat ccgcgagctg accgacgggc cattcgggct
     2041 caaccacctg ctggccttcg ccaccgagga ccggtttgcg gtgacgctcg aggcgcggcc
     2101 tcgggtggtc tccaccgcgt gggcgtgggc cgagcaggac ctgcacccgt atttcgaccg
     2161 ggcgcacgag gccggagcat tggtgatgca catggtctcg accgttcccg aggcggtgcg
     2221 cgcggtcggc gccggggcgg acatcattgt cgcgcagggg accgagggcg gtggtcacgt
     2281 cggctggatc agcacgctgc cgctcgtgcc ccaggtcgtc cgggccgtgg cgcccgtgcc
     2341 ggtgctggcg gccggtggga tcgccgacgg ggcggggctg gcggcggcgc tggcgctcgg
     2401 ggccgagggc gtcctcctcg ggacccggtt tctcgccacc cctgaggccc cgatcccggc
     2461 ggggtacaag gaagcgatcc tcgccagcga tgggcacaac accctgctca cagaggtggc
     2521 cgatatcgcg tttggacgcg tctggccagg cgcgatgtcc cgtgtcatcc gcaaccagtt
     2581 tgtcgaacgg tgggtcggtc gcgagtggga ggtgcgccag cggcaccacg aaatcgcccg
     2641 cgggttgcgc gcggcgcggg agcgcgacga cgcaggggag tcgaacttac agatgggtca
     2701 ggcggcggga ttgatcaccg aggtcgctcc cgccgccgac atcgtccgcg agatcgtcgc
     2761 gcaggccgag cggatcatcc gaagccgcct cccgagcctg ctggccgact gagcgccggt
     2821 cctcgacgct accgccgcgc ggccgaggag accgacggga tcggccggcg gagccggccc
     2881 cgggcaacca ggtagtcgac gagcaccgcg tacgggtcga accccacgat gcgcggggca
     2941 tcggtgacaa agttcgagag cgcgttgatg tcgtaatagt agacctcgcc gtcccgatcg
     3001 ttcacgagat actcgacgcc gccgaggtcg atgcgggccg cccgggtgat cgccacgacg
     3061 tcctgggtca cggcctcggg cggggtgtag ccttccaccc tgagcgcgac tttggggagg
     3121 tcgacagggc acagatccgc cgaggtcgcc cgggctgccg gctctgcgcc ggcgtcgggg
     3181 tgacagatgt cggccgggca gaggttgaac cccgcctcgg ggttcggata caccttgatg
     3241 gcgtagagga accggccgtc caggacctcg acgcggacga tgtgcccgcc acgggcgggg
     3301 agaaactcct ggacgagccc gatgcgatcg acgcccaatg cgagcgcgcc ggcctccgct
     3361 gcgcgcccga gggcctcggg ggactcgaac cgctgcatcc cggctccgct tccgccgatg
     3421 ttcggcttga cgatgacggg gaagtcgagg ccggccgccg gggccggggc ctgggacggg
     3481 tgattgatca ccagggtccg cggtgcccgg aggcccaact gccgaagcaa gaggacctgc
     3541 gcgaccttcg agatctccag ggtgaaggcg gccgagccgt tcaccacatc ggtccccgcc
     3601 gcttccaggt agcggagaaa ctcgcgcccg taggcaatgg cctgcccgtg gccgcgcagg
     3661 tccgccgatg ggctcaggcg gttgaggacc agggaccatg ggacggcgcg gcggtcgggg
     3721 tcgaacgtgt gcgtgcctag gtcgacgcgg tcgtacccga tccgccggcg atccagctcc
     3781 gcaaacagcg gcgcgaacca tgtggggtgc tcatacacga cgccaaagcg aggccctgct
     3841 gtcctcattg gatcctcctt cgattgaaag gccgtagggg ctagagccgg aagcggaggt
     3901 gggttacctc cgcggaggac agatgcactc gcgagggcgc tgcatagtgc ctgcgatact
     3961 atcaaatggc gcagccgggc acaaggcggg ctagaagacc ctgcccccgg cgggcacctc
     4021 gcgctcggtc gagagcagca ccacccggcc cgtgtcgtcc gggaccccca gcaccagcac
     4081 ctccgaggtg aacccggcga tccggcgggg accgaggttc atcgcggcca ccacggtgcg
     4141 tccgatgagg cgctcggggg tgtagagatc cgtgacctg
//
LOCUS       DASQGH010000243         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_6816, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000243 DASQGH010000000
VERSION     DASQGH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6816"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VEW91_04650"
     CDS             <1..790
                     /locus_tag="VEW91_04650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HYM90906.1"
                     /translation="GAIAPGARIAVYFAPNTDRGFLDAITTAVHDSTNNPSAISISWG
                     AAEANWTQQAMLSFDQAFQEAAALGVTVCCAAGDDGSSDGVQDGAPHVDFPAASTFVL
                     GCGGTSLASTGTSITSEVVWNDPGDGATGGGVSRQFALPSWQANASVPPSPAPGSGGR
                     GVPDVAGDASPASGYTVLVDGLWIVIGGTSAVAPLWAGLIALLNEQKGKPMGYLNPVL
                     YGLMSKGVFHDITQGNNGAYTAGPGWDPCTGLGTPDGTKLLSAL"
     gene            941..2116
                     /locus_tag="VEW91_04655"
     CDS             941..2116
                     /locus_tag="VEW91_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90907.1"
                     /translation="MRKATALLLGALLPLLGLAGVGQAQSSVQIQGSIQAVDCEARTI
                     VLSSAGGSNTVAAADYTPVLVNSASVPFCTLQQYFGAPATAWLVASNSQFVAVRIDIT
                     GQPAGAYPPPAPVVVAPAPLPIAGIVLGTIIVAGLVYLLARDYDGHYYRYPYYGPYYR
                     HYYRPEYRPYLGPYPVLGPVIVAPAPIAGVVLGTIIIAGLAYRVARDHDGRYYRYPYY
                     GPYHRYYYRPEYRPYQGPYRDAPVREGDSHWDVQSRQSSPVPHGVPASHSPTWNVPAP
                     QNDPHRDAPASHDPVHVPAPQAPQGGPQWSPPAHPDKGGPGYRDSGGRAPVPVSAPQR
                     GPQPPRTAPAYHQGDGHPPAYPSGHREAAPRYHGGGAQCGGRGSDQGCSGGNHASDR"
     gene            complement(2137..>2948)
                     /locus_tag="VEW91_04660"
     CDS             complement(2137..>2948)
                     /locus_tag="VEW91_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM90908.1"
                     /translation="DMERLGVDLQIVSVTIPNVYFGPPARRRDLARMANDGLAELTRR
                     FPERFAALASVPLEDPEAAIQELERAVLQLGMVGVILGSNAGGRYLDDPAFFPFYERV
                     EALKVPILIHPMPPLEADPTFARGLVPLLGFVFNTSTSVARMVLAGIFERLPGLTLIL
                     GHLGGTLPYLMQRLDNGYRAYPEARGALPHPPSHYLRRLYYDTVSFSIPSLRCALGAV
                     GPEQIVMGTDYPHVIGDIRAALGSVRALALPDPAVAAILGGTAVRLFPRLA"
BASE COUNT          460 a         1004 c         1021 g          463 t
ORIGIN      
        1 cggggccatc gctccgggcg cgcgcatcgc ggtgtatttt gcccccaaca cggaccgcgg
       61 ctttctcgat gccatcacga cggccgtcca cgacagcacc aacaaccctt cggccatctc
      121 gatcagctgg ggggcggccg aggcgaactg gacgcagcag gccatgctgt ccttcgatca
      181 ggccttccaa gaggccgccg ccctgggcgt gaccgtgtgc tgcgccgcgg gagacgacgg
      241 ctccagcgac ggcgtgcagg acggcgcgcc gcacgttgac ttccccgccg cgagcacgtt
      301 cgtgctgggg tgtggcggca cgagcctggc gtcaaccggg accagcatca cgagcgaagt
      361 cgtgtggaac gaccccggcg acggtgccac cggcggcggg gtcagccggc agttcgcgtt
      421 gccgtcctgg caggcgaatg cgagcgtgcc cccttcgccg gcgcccgggt ctggaggtcg
      481 gggagtcccc gatgtcgccg gcgatgcctc gcctgcgtca ggctacaccg tcctggtgga
      541 cgggctatgg attgttatcg gtgggaccag cgccgtggcg cctctgtggg ccgggctcat
      601 cgcgctgctg aacgagcaga agggcaagcc catgggctat ctcaaccccg tgctctatgg
      661 tctgatgagc aagggcgtgt tccacgacat cacccagggc aataatggcg cctataccgc
      721 cggcccgggg tgggatccct gcacaggtct cggcaccccc gatggtacga agctcctgag
      781 tgcgctctga gggcgagcgg ggccggtaga gcaggtgccc catcttcaca acttcttcac
      841 gggttattca cgcttggttg gtacactttg accgagtgac ggattggccc cgcgggttcg
      901 cgccgccggc cgatgcgatg gcgtgaagga ggggtgcgcg atgcgaaaag caaccgcact
      961 gctgcttggg gcgcttctgc cgctcctggg gttggcgggg gtcggccagg ctcaatcatc
     1021 tgtgcagatc cagggcagca ttcaggccgt cgattgcgag gcgagaacga tcgtcctgag
     1081 cagcgccggt ggttcgaaca cggtcgcagc ggcggactac actcccgtgt tggtcaactc
     1141 ggcgagcgtg ccattctgta ccctgcagca gtacttcggt gccccggcga ccgcgtggct
     1201 cgtggccagc aacagccagt ttgtggccgt gcggattgac atcaccgggc agccggctgg
     1261 ggcgtacccg ccgcccgcgc ccgtggtggt ggctccggcg ccactcccca tcgcggggat
     1321 cgtgctcggc acgatcatcg tcgcagggct ggtgtacctg ctcgcccgtg actacgacgg
     1381 acactactac cggtatccgt actacggtcc gtactaccgg cactattacc gccccgagta
     1441 tcgtccctat ctcgggccgt atcccgtgct ggggccggtc atcgtagccc ccgcgccgat
     1501 cgcaggcgtt gtgctcggca cgatcatcat cgcagggctc gcgtatcggg tcgcgcgcga
     1561 ccacgacgga cgctactacc ggtatccgta ctacggtccg taccaccggt attactacag
     1621 accggagtat cggccctatc aggggccgta tcgcgatgct ccggtgcggg agggcgattc
     1681 ccactgggac gttcaatccc gccagagcag cccggtgccc cacggtgttc ccgcgtccca
     1741 cagcccgact tggaatgttc ccgcacccca gaacgacccc catcgggatg ctccggcttc
     1801 ccacgatccg gtccacgttc cggcgccgca agcgccgcag ggcggcccgc agtggagccc
     1861 gccggctcat ccggataagg gcggtcctgg ctatcgcgac tctggcggcc gcgctccggt
     1921 gccggtgagc gccccgcagc gggggcctca gcccccccgg acggctccgg cctatcatca
     1981 gggggacgga catccacccg cgtacccgag tggccatcgt gaggccgcac cccgatacca
     2041 cggaggcggc gcgcagtgcg gaggccgggg gtcggatcag ggatgctcgg ggggcaacca
     2101 cgcttctgac aggtgacgcg ccgaccccgg cgctcgtcag gcgagacgcg ggaacaatct
     2161 gactgcggtt cctcccagga tcgctgcgac cgccgggtcg gggagggcga gcgcgcgaac
     2221 gctccccagg gcggcgcgga tgtcgccgat cacatgcggg tagtccgtgc ccatcacaat
     2281 ctgctccggc cccaccgccc caagcgcgca ccgaagcgac gggatcgaaa acgagacggt
     2341 atcgtagtac aggcgcctga ggtaatggct ggggggatgc gggagcgctc cccgggcctc
     2401 cgggtacgcc cggtagccgt tgtcgagccg ctgcatcagg tacggtagcg tcccgccgag
     2461 gtgccccagg atgagcgtga gcccgggcag ccgctcgaag atccccgcca ggaccattcg
     2521 ggcgacgctc gtcgaggtgt tgaacacgaa gccgaggagg ggcacgagcc cgcgcgcgaa
     2581 ggtgggatcc gcctcaaggg ggggcatcgg gtggatgagg atgggcacct tgagcgcctc
     2641 cacccgttcg taaaacggga agaacgccgg gtcatcgagg tagcggccgc cggcgttgct
     2701 gcccaggatg acgcccacca tccccagttg gagcaccgcc cgctcgagtt cctggatcgc
     2761 ggcctcgggg tcctcgagcg ggacgctcgc cagcgccgcg aacctttccg ggaagcgccg
     2821 ggtgagctcc gccagcccgt cgttggccat ccgggccagg tcacggcggc gggccggggg
     2881 gccgaagtag acgttcggga tcgtgaccga cacgatctgg agatcgaccc caagccgctc
     2941 catgtccc
//
LOCUS       DASQGH010000244         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49229, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000244 DASQGH010000000
VERSION     DASQGH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49229"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VEW91_04665"
     CDS             complement(<1..918)
                     /locus_tag="VEW91_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM90909.1"
                     /translation="MIDSHAHFVPPDLAARIAADGVRLGVRLGSDGRVAFASGETSRP
                     LSPALLTSRRPAGCDVQIVSPWIDLVGYGLDAERGAGWARLLNTAMAGRPALATVPLQ
                     DGRAAAGELSWALGHGFLGVEIGTNVRGRELSDPGLEPFWAACAEARTPVFFHPAYVI
                     ATPRVASHYLHNLAANPFDTTLAVAQLIFGGVLDRHPDLVLFLAHGGGYWPYQFGRLD
                     RGHAVRPECRGAAQAPRAYLRAFYYDTVVHSPEALRYLIGLVGADRVLLGTDMPFDMG
                     DPEPLVTLDGAGITPHEHQAIVHYNAAFLF"
     gene            complement(1077..1397)
                     /locus_tag="VEW91_04670"
     CDS             complement(1077..1397)
                     /locus_tag="VEW91_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HYM90910.1"
                     /translation="MPKFLVQASYTPEGARGVIKGGGAARRAAVQQMAQGLEGKVDAF
                     YFGFGSDDAFVILDLPDNISMAACALAVNAAGAAHIKTTVLLTPEELDQAARKTVHYR
                     SPGQ"
     gene            2319..3422
                     /locus_tag="VEW91_04675"
     CDS             2319..3422
                     /locus_tag="VEW91_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYM90911.1"
                     /translation="MGDPRIAQLARVLAHYSLRLKKGQLVRISGPAVAAPHIAAAYRE
                     ALLAGAYPVVRAGVEGLEELYFKHASDEQLRFISDLERLEVEKFDADLRFLGSYNTRA
                     LTAVPPERMARRREATRVLTNRFLERAARGDLRWCLTQAPTHADAQEAEMSLAEYEDF
                     VYSAGHLDREDPVAAWEAVAKEQAKVVALLSPVKTIRVRGPDTDLTVSVAGRRWISAA
                     GEHNFPDGEVFTGPLEEATRGTVRFSFPAIYGGREVTDVRLTFEGGRVTDASAAKGED
                     FLRAMLDLDGGARTLGEFAFGLNYDIQRWTRNILFDEKIGGTLHMALGAAYPDTGGRN
                     TSGLHWDMILELRNGAEVYGDGEVIHRDGRFLM"
     gene            complement(3429..3713)
                     /locus_tag="VEW91_04680"
     CDS             complement(3429..3713)
                     /locus_tag="VEW91_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90912.1"
                     /translation="MNEPLWDLYQRVCQEEVRPLEEFVERLLAKEWGPYPREDILELL
                     HEIEGQMLANIQVKALEGPRFAEMADEVSEQTQREFEGLVGRVERAFSGG"
BASE COUNT          673 a         1350 c         1346 g          628 t
ORIGIN      
        1 gaagaggaag gcggcgttgt agtgtacgat ggcctgatgc tcgtgcggag tgatcccggc
       61 gccgtccagg gtcacgaggg gttccgggtc gcccatatcg aacggcatgt ccgtccccag
      121 gagcacacgg tccgctccca ccaggccgat gaggtagcgc aacgcctcgg gcgagtggac
      181 cacggtgtcg tagtaaaacg cgcgcagata ggcacggggc gcctgcgcag ctccgcggca
      241 ctcggggcgc accgcgtgcc cgcggtcgag ccggccaaac tggtacggcc agtacccacc
      301 cccgtgggct agaaacaaga cgagatccgg gtgccggtcc aagacgccgc cgaaaatcaa
      361 ctgcgccacc gcgagggtcg tgtcgaacgg gttggccgcc aggttgtgca ggtagtgcga
      421 tgcgacccgc ggggtggcga tcacataggc cgggtggaaa aagacgggcg tccgcgcttc
      481 cgcacacgcg gcccagaacg gttcgagccc tggatcggac aactcgcggc cccgcacgtt
      541 ggtgccgatc tccaccccca ggaagccgtg gcccagcgcc caggagagct cccccgccgc
      601 cgcgcgccca tcctggaggg gcaccgtcgc cagggcgggt cggcccgcca tcgcggtatt
      661 cagcagccgc gcccagcccg caccccgctc cgcgtccaac ccgtagccca ctaggtcgat
      721 ccagggcgac acgatctgca cgtcacatcc ggcgggccgg cgagaggtca gcagggccgg
      781 ggacagcggc cggctggtct ctcccgatgc aaacgcgacc cggccgtcgg atcccagccg
      841 gacgccgagc cggaccccgt ccgcagcgat gcgggcggcg agatcgggcg ggacgaagtg
      901 cgcatgcgag tcgatcatgg ggacgcttca cctctttggc cttcacagga ccgccctcct
      961 gccgccgggt ggaaacgaag gaggcccctc ccgcggaagg gcctccctga gacccacgac
     1021 agagaacttc tcaggcgatt ccgagaaccc tgtggcgaag ctcctttccg gatcccctac
     1081 tgtccgggcg accgatagtg aacggtcttc ctcgcagcct ggtcgagctc ctcgggggtg
     1141 aggagcacag tggtcttgat gtgggctgcc ccggccgcgt tgacggccag cgcgcaggca
     1201 gccatgctga tgttgtccgg aagatccaga atcacgaaag cgtcatcgct cccgaaccca
     1261 aaatagaacg catcgacctt cccctccaga ccctgggcca tctgctggac cgcggcccga
     1321 cgtgccgcac cgcctccctt gattaccccg cgtgcaccct cgggcgtata cgaagcctgc
     1381 acgaggaact tgggcatagg tcacctcccc tgaggatttg ttggtggcac ttccctgggc
     1441 cagaacatct gatgtgagaa cctgggtgcg ttgtgcggaa gaaggagtgg gattttgtcc
     1501 aagccagctg cgtgaatccc tgcgtgtcgt tgcgatgcaa tttctcgccg tgcaatctaa
     1561 taatgcaccg accgcgccgg agcagcaatg gggaacgcgc gtgcgctccg cggggcttcc
     1621 ttgcgccgat cagcggtcca tccgatcccg atacagatcg cgccggtcgc gccggatgtc
     1681 ccaggtatca tggcgcaggt cccgcgcgtc gcgcgctatg ccgctgcggt tcccgccgtg
     1741 cgccgttctt gctctcggcc atcgcgacgc ttgggagcat gatgcttggg gcgaccatcc
     1801 cccaccgcag aggtcccgcg gtggcgtctg ggcggacaga cggccgggct cgagcggggg
     1861 cgtgcgggtg agaccatatt ctcgattcgg tggagccacc gggtattcgt ccggatgggt
     1921 acgcccgttc tagcccctgc ttcacagatg gttgcaacca aggatgagag gctcgggtcc
     1981 tggaattctg gggaattccg accctatggc tgggcccctc ggattatcac agatttttga
     2041 aaatttcctc caattcccag gagggaaaaa gtaaagaatg tcgaatacaa ggtatccgag
     2101 gggcagaagc gaggccccct gagataccaa agtcgaaagg agggccctct ccgtggcgaa
     2161 gaagaagaag gcgggcggca aaaagaagag atagtctttt cgccctggat ccgatcgggg
     2221 gcgttgtgca taagcacggc gcccccctgg ctttttgtcg cgcgcacagg ggtgatgcct
     2281 ccgcaggaga tcgcgccgca acgcggaact gacgctgcat gggtgatccc agaattgcac
     2341 agctcgcccg cgtcctcgcc cactactccc tccggctcaa gaaaggccag ctggtacgga
     2401 tcagcgggcc cgcggttgcc gcgccgcaca tcgccgccgc ctaccgggag gcgctcctcg
     2461 ccggggcgta ccccgtcgtt cgcgccgggg tggaagggct cgaagagctc tacttcaagc
     2521 acgcgagcga cgagcagctc cgcttcatct cagacctgga gcgcctggag gtcgagaagt
     2581 tcgacgccga cctcaggttc ctcggctcct acaacacccg ggccctgacc gcagttcccc
     2641 cggagcggat ggcgcgacgg cgggaggcga cccgggtgct gaccaaccgg ttcctcgagc
     2701 gtgcggcccg gggagacctc cggtggtgtc tcacgcaggc gccgacccac gccgacgcac
     2761 aggaagccga gatgtcgctc gccgagtacg aggacttcgt ctactccgcc gggcacctgg
     2821 accgggagga tccggtcgcg gcctgggagg cagtggcgaa ggagcaggcg aaggtggtcg
     2881 cgctcctgag cccggtcaag accatccgcg tgcgcggtcc ggacaccgac ctgacggtgt
     2941 ccgtggccgg gcggcggtgg atcagcgcgg cgggcgagca caacttcccc gacggggagg
     3001 tcttcaccgg ccctctcgaa gaggcgaccc gggggaccgt ccggttctcg tttccggcga
     3061 tctacggcgg ccgggaggtc accgacgtgc ggctgacatt cgaggggggg cgcgtcaccg
     3121 acgccagcgc cgcgaagggg gaggatttcc ttcgggcgat gctggacctt gacggcgggg
     3181 cgcggaccct cggggagttc gccttcggcc tcaactacga catccagcgg tggacccgga
     3241 acatcttgtt tgatgagaag atcgggggga ccctccacat ggccctcggc gccgcgtacc
     3301 ccgacaccgg gggacggaac acctcggggc tccactggga catgatcctt gagctgcgga
     3361 acggggcgga ggtctacggg gacggcgagg tgatccaccg ggacggccgg tttctcatgt
     3421 aggcccggtc accctccgga gaacgcccgc tcgacccgcc cgaccaagcc ctcgaactcc
     3481 cgctgggtct gctcgctcac ttcgtcggcc atttcagcga accgcggccc ctcgagggcc
     3541 ttcacctgga tgttggcgag catctgccct tcgatctcgt ggagtaattc caggatgtcc
     3601 tcccgggggt acggccccca ttccttcgcc agcagccgct ccacgaactc ttcgagcggg
     3661 cgcacctcct cctggcacac ccgctggtag aggtcccaca gcggctcatt caccggcgtc
     3721 ggcccccgga cacacaggtc cagggtatac tgaggagagc ccgcggttcc ctgtccgggc
     3781 cccgtccccc ccggcggtcg ctgccgggac caggaggagc cggtgccgag aatgttctgc
     3841 atccgatgcg gcgcgcgcct gcggcgcgtg cgcgaacatg gccgcgcccg ccgcgtcggc
     3901 ggctgccccc ggtgcaggtg ggtcgactgg aacaatccgg ccccgaccgc ttcggtgctc
     3961 gtgctgcgcc gcagccgcgt cctgctcgtc cggaggg
//
LOCUS       DASQGH010000245         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43511, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000245 DASQGH010000000
VERSION     DASQGH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43511"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VEW91_04685"
     CDS             <1..522
                     /locus_tag="VEW91_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYM90913.1"
                     /translation="LPDGARVTLRPLVAKDEEKLVQLFADIPYEELRNLQDNVSDPVI
                     VRRWCRNINYDRVLPIIAELDGRMVADATLHRRTVAPLREIGRVRAYVRPEYRRRGLG
                     TVLLREIMGLARQLGLRRLAVELYEDQTALRSVFVRYGFSEEGRVPAYQRVILVREIA
                     EQENEAPGAEETP"
     gene            complement(766..1302)
                     /locus_tag="VEW91_04690"
     CDS             complement(766..1302)
                     /locus_tag="VEW91_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYM90914.1"
                     /translation="MRWTIDPVHSSIEFAILHMSISKIRGRFRKLRGVIETSDDGVLQ
                     TINLMIEAGSIDTGDSRRDAHLISPDFLDAAQHPSLVFRSTKVDALGNGRYRVGGTTT
                     IRGRTRPMTIEVATTNPVTDAEGNRRAGARAEGRLSRMDWGLTWNSPLPWGGLLVGDT
                     VWFTIDVEAVAFATVAVA"
     gene            complement(1334..1639)
                     /locus_tag="VEW91_04695"
     CDS             complement(1334..1639)
                     /locus_tag="VEW91_04695"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90915.1"
                     /translation="MANAVATRPIRHGVQEVVFRFDGRRVPRARAVALVGSFNRWDTA
                     VHPLTLGPDQWWTISLTLAPGRYPYLFIVDGVPWNDPKDDARAPCEWGGEYSVRIVP"
     gene            complement(1718..3070)
                     /locus_tag="VEW91_04700"
     CDS             complement(1718..3070)
                     /locus_tag="VEW91_04700"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HYM90916.1"
                     /translation="MSDSRSDFVTERTMALLTDLYELTMAQSYLRGGRNEVSTFDLFV
                     RDLPPHRSFLVSAGVDTVLTYLERVRFSDDAIGYLRTLALFDDAFLEYLRAFTFTGEV
                     RAIPEGEVVFPPEVLLEVTAPRIEAQIVETFLLNQMNFQTMVASKAARVVLAARGRPV
                     VDFSPRRDHGTDAAMKVARCAYLAGCTGTSNVLAGMAYGIPVYGTMAHSYVMSFDDEL
                     EAFRAYAQHFPQQCLLLIDTYDTVQGARNAAVVATELRSRGSRLLGVRLDSGDLVDLS
                     RAVRRVLDDAGFPDAQILASGDLNEEKIRDLLDAGAPVDMFGVGTEMGVSYDAPALGG
                     VYKLVEDTRGYRIKRSAGKATLPGRKQIWRVVRDGQMLSDTITLHDEPAPPGARPLLT
                     EVLRGGRRTRRESLEEARHRCLDRVASLPPPHRELNGAAYPVRLSPKLDALRREMAGP
                     "
     gene            complement(3092..3781)
                     /locus_tag="VEW91_04705"
     CDS             complement(3092..3781)
                     /locus_tag="VEW91_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYM90917.1"
                     /translation="MNGPPRPRGTDNLVIVRAGPVALEGDLRIPEGARGVVLFAHGSG
                     SSRHSPRNRFVAEFLRDARLGTLLIDLLTAQEETAEAATGHLRFDIDLLAGRLVAISD
                     WLRGHPATGPLALGYFGASTGAAAALVAAAQRPEDVRAVVSRGGRPDLAWSALPQVTA
                     PTLLIVGERDSTVLDLNREALRALHVEKALAVVPRATHLFEEPGALEEVARLAREWFS
                     RHLTPGDDVER"
BASE COUNT          624 a         1280 c         1323 g          578 t
ORIGIN      
        1 cttcccgacg gcgcccgggt gacgctccgg ccgctggtgg ccaaggacga ggagaagctc
       61 gtgcagctgt tcgccgacat tccttatgag gagctgcgaa atcttcagga taacgtcagc
      121 gatccggtga tcgtccgacg atggtgccga aatatcaatt acgatcgggt gctccccatc
      181 atcgccgaac tggacggccg gatggtggcc gacgccaccc tgcatcggcg gaccgttgcg
      241 ccgctgcgcg agatcggaag ggttcgggcc tacgttcggc ccgaataccg gcggcgcgga
      301 ctcggcaccg tgctccttcg ggagatcatg ggtctggcgc gtcagttggg gctcaggcgg
      361 ctggccgttg agctgtacga agatcagacg gcgttgagaa gtgtgttcgt gcgatacgga
      421 ttctcagagg agggccgcgt cccggcctac cagcgggtca tcctggtgcg cgagatcgca
      481 gagcaggaga acgaggcccc gggcgcagag gaaaccccgt gaagaggcgg atgctcgcac
      541 gaatcacggt ggagaaggcg aagcgcaccg ggaaacggcg gcgtcaccat gctgcatccc
      601 gggccagaga ccccaaggat ctgaggggcg ccaaggggat cttgctgaag agtgcggcgg
      661 aagctgccct gccggatcaa ctcgccgagg cccagggggc cgaccagcga catcctccga
      721 tccgaatccg aagcgactga gccgtagggc tcatcgcgca ggggtctacg ctacggctac
      781 cgtggcgaag gccaccgcct ccacatcgat cgtaaaccag acggtgtccc ccaccaggag
      841 gccaccccac ggcagggggg aattccaggt gaggccccag tccatacggg agagccgccc
      901 ctcggcgcgg gcccctgccc ggcggttgcc ttcggcgtcg gtgaccggat tggtcgtggc
      961 tacctcgatc gtcatcggcc gggtccggcc gcgaatcgtg gtagtccccc ctacccggta
     1021 gcgcccgttc cccagggcat caaccttggt gctgcggaac accaggctgg gatgctgcgc
     1081 ggcatcgaga aagtccggcg agatcaggtg cgcatcccgc cgggagtcgc cggtatcgat
     1141 gctcccggcc tcgatcatca ggttgatcgt ctgaagaaca ccgtcgtcgc tggtctcgat
     1201 cacgccccgc agcttgcgga agcggcctcg gatcttcgag atcgacatgt ggagaatggc
     1261 gaactcgatc gatgagtgga caggatcgat ggtccaccgc atcttccgcc cctcccgtcg
     1321 caggcgccgc ggcctagggg acgatccgga ccgagtactc cccgccccac tcgcacggcg
     1381 cccgcgcgtc atctttgggg tcgttccacg gcaccccatc gacgatgaac aggtacggat
     1441 accgtcctgg agccagggtg agggaaatcg tccaccactg gtcgggaccc agcgtcagcg
     1501 gatgcaccgc cgtatcccac cggttgaacg agcccaccag cgccacggcc cgcgcgcggg
     1561 ggacgcgccg gccgtcgaac ctgaagacca cttcttgcac gccatgccgg atcggccgag
     1621 tcgcaaccgc gttcgccatt tcggaccctc caccactttc taccgtcaag atagccgcgg
     1681 ggcacgcggt tgggaatcag gcggagaccc gataccctta cgggcccgcc atctcgcggc
     1741 ggagggcgtc gagtttcggg ctgagccgca cgggatacgc ggccccgttg agctcccgat
     1801 gcggcggggg caacgaagcc acgcggtcaa ggcaacggtg gcgcgcctct tctagggact
     1861 cccggcgcgt gcgccggcct ccccgcagaa cctctgtgag caagggccgg gcgccggggg
     1921 gtgccggctc gtcgtgcagg gtgatcgtgt ccgagagcat ctggccgtcc cggacgacgc
     1981 gccagatctg cttccggccc ggcagggtgg ctttcccggc gctccgcttg atccggtagc
     2041 ctcgggtgtc ctcgacgagc ttgtagacgc cccccagcgc gggagcgtcg tacgagaccc
     2101 ccatctcggt gccgaccccg aacatgtcca ccggcgctcc cgcatcgagg aggtcccgga
     2161 tcttctcctc gttgagatcg ccgctggcca ggatctgcgc gtccggaaag cccgcgtcgt
     2221 cgaggacccg ccgtaccgcg cggctgagat ccacgaggtc gccgctgtcg agacggacgc
     2281 cgaggagacg gctcccccga gaccgcagtt cggtcgcgac gaccgccgcg ttccgcgcgc
     2341 cctgcacggt gtcgtaggtg tcgatgagga gcaggcactg ctgcgggaaa tgctgcgcgt
     2401 acgcccggaa ggcctccagt tcgtcgtcga acgacatcac gtaggagtgc gccatggtgc
     2461 cgtagaccgg aatcccgtac gccatgcccg cgaggacgtt ggaggtgccg gtgcagcccg
     2521 cgagataggc gcaccgggcg accttcatgg cagcgtccgt gccgtggtcc cggcgcggag
     2581 agaaatccac caccggccgg ccccgggcgg ccagcacgac gcgggcggct ttgctggcga
     2641 ccatcgtctg gaagttcatc tggttcagca ggaaggtctc gacgatctgc gcttcgatgc
     2701 ggggagcggt gacctcgagc agtacctcgg gagggaagac gacctcgcct tccgggatcg
     2761 cccgcacctc cccggtgaag gtgaatgccc ggaggtactc cagaaacgca tcatcgaaca
     2821 acgccagcgt ccgcaggtag ccgattgcgt cgtcggagaa gcggacccgc tcgaggtacg
     2881 tcagcaccgt gtcgacgccc gcgctcacga gaaagctgcg gtggggcggc aggtcgcgca
     2941 cgaacaggtc gaacgtcgaa acctcgttgc ggccgccgcg gaggtagctc tgcgccatcg
     3001 tcagctcata caggtccgtg agcagcgcca tcgtgcgctc ggtcacgaaa tccgaacggg
     3061 agtcgctcat cgccgcgtcc gcggtccggg gtcagcgttc gacgtcgtcg cctggggtga
     3121 ggtgccggct gaaccactcg cgcgcgagcc gtgcgacctc ttctagggct cccggttcct
     3181 cgaacaggtg cgtggccctc gggacgacgg ccagggcctt ctccacgtgc agcgcccgca
     3241 gcgcctcccg attcaggtcg agcacggtag agtcacgctc gcccacgatc aacagcgtgg
     3301 gagccgtcac ctgggggagc gctgaccacg cgagatcggg gcggcctccc cgggagacga
     3361 ctgcacggac gtcttcgggt cgctgtgctg cggcgacaag cgccgccgcc gccccggtgc
     3421 tggccccgaa gtagccgagc gcgagagggc cggtcgccgg atgcccgcgc agccagtcgc
     3481 tgattgcgac gaggcgcccg gcgagcagat cgatgtcgaa ccggaggtgc ccggtggcgg
     3541 cttccgcggt ctcctcctgg gcggtcagca ggtcgatcag gagcgtgccg aggcgggcgt
     3601 cgcggaggaa ttcggccacg aagcggttcc gcgggctgtg gcggctgctt ccgctgccgt
     3661 gggcgaacag cacgacgccc cgggcgcctt cggggatgcg caggtcaccc tcgagcgcca
     3721 ccgggccggc ccggacgatg acgaggttgt ccgtccctcg gggccgcggc ggtccgttca
     3781 tggtacgcct ccaggtggct gcagc
//
LOCUS       DASQGH010000246         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54872, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000246 DASQGH010000000
VERSION     DASQGH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54872"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VEW91_04710"
     CDS             <1..351
                     /locus_tag="VEW91_04710"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYM90918.1"
                     /translation="HLPPFLVRAGARAPVPLETPGLLLGVEPDTAYVPAEVPVGPGDV
                     LVMFTDGITEARSPNGEEFGDQRLSALLEGLAAVGPDEVADRVMDGVASWSPGTPADD
                     QTIVVVRIDPPAVD"
     gene            481..1041
                     /gene="infC"
                     /locus_tag="VEW91_04715"
     CDS             481..1041
                     /gene="infC"
                     /locus_tag="VEW91_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYM90919.1"
                     /translation="MALVPSPPCLKEVRESIERGARINDRIRAREVRLIGANGEQLGV
                     IPVAEALAKADEANLDLVEVAPTATPPVCRIMDYGKYKYEQAKREREAHKKSKTSGLK
                     GMRLSPKIGQHDLQVKTRAVSGFLRDGDKVRVSMWFRGREMAHPHVGEQILRRMAQDL
                     ADVGVVERHPLMEGRNMIMILSPKRN"
     gene            1042..1239
                     /gene="rpmI"
                     /locus_tag="VEW91_04720"
     CDS             1042..1239
                     /gene="rpmI"
                     /locus_tag="VEW91_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HYM90920.1"
                     /translation="MGKLKTHQGTAKRIRVTGRKRLLRGRQLGGHLMVHKGPKRKRSL
                     RENRALEPADLARVERLLPYR"
     gene            1276..1629
                     /gene="rplT"
                     /locus_tag="VEW91_04725"
     CDS             1276..1629
                     /gene="rplT"
                     /locus_tag="VEW91_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HYM90921.1"
                     /translation="MTRVKRGVVVRRRHRKVLRLARGYWGKKSRWFKLASQTVKRALA
                     QAYAHRRARKREFRQLWIARINAAARTHGLSYSRLIFGLRRAGIEVNRKMLADLAVED
                     GQAFSALVKRAQRTE"
     gene            1692..>2130
                     /locus_tag="VEW91_04730"
     CDS             1692..>2130
                     /locus_tag="VEW91_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HYM90922.1"
                     /translation="MAGDVLETIITSRKNPVIEDLRSLLRSSTRRTARCVVEGWHFLE
                     TAAARGAECLLVVHTPAAAADPRAGELRQRLHAGGARLVVVSPYVFEALTQVETPQGV
                     LGVLRRPPAASPTVLRDPRLLLVVLDGVQDPGNVGAVLRTAAAA"
BASE COUNT          336 a          679 c          794 g          321 t
ORIGIN      
        1 cacctcccgc cgttcttggt gcgcgcggga gcgcgcgcgc ccgtgcccct cgagaccccc
       61 ggcctgctgc tcggggtgga acccgatacg gcgtatgtgc cggcggaggt gcctgtgggc
      121 ccgggggatg tgctcgtgat gttcacggat gggattacgg aagcgcggag ccccaacggg
      181 gaggagtttg gggaccagcg cctctcggcg ttgctcgagg ggctggccgc ggtcgggccc
      241 gatgaggtgg ccgatcgggt gatggacggg gtggcgtcgt ggtccccggg cacgcccgcc
      301 gacgaccaga cgattgtcgt cgtccgcatc gatcccccgg ccgttgattg acgctgttga
      361 gaggcctgtg ctatactggc accgcttgaa aagcagaagc catctgcttc tcaccctggg
      421 gcgcggccgg acatccggtc gcagctacca gggtttcggt tcggagacga tccggagggg
      481 atggcattgg tgccatcccc tccgtgcctg aaggaggttc gagagtccat cgagcgcggc
      541 gcgcgcatca acgaccgaat ccgggcccgt gaggtccggc tgatcggggc gaacggcgag
      601 cagctggggg tcataccggt tgcggaggcc ctggcgaagg cggacgaggc gaaccttgat
      661 ctcgtcgagg tggccccgac cgcgacccct ccggtctgcc ggatcatgga ttacggaaag
      721 tacaagtacg agcaggcgaa gcgggagcgg gaagcgcaca agaagtccaa gacctccggg
      781 ctgaagggca tgcggttgag cccgaagatc ggtcagcacg acctgcaggt gaagacgaga
      841 gcggtgagcg ggttcctccg ggacggggac aaggtgcgtg tctccatgtg gtttcggggt
      901 cgagagatgg cgcaccccca cgtcggggag cagatcctgc gccgcatggc ccaggacctg
      961 gccgatgtcg gggtcgtgga gcggcatccc ttgatggaag ggcgcaacat gatcatgatt
     1021 ctctccccga agaggaacta agtggggaag ctcaagaccc atcaggggac ggccaagcgc
     1081 atccgggtga ccgggcggaa acggctcctc cgggggcgcc agctgggcgg ccatctgatg
     1141 gtgcacaagg gacccaaacg caagcggtcg ctgcgggaga atcgcgcgct cgagcccgcc
     1201 gacctcgcca gggtcgagcg gctgctgccg taccggtagc gcccgctcgg gagagcccgc
     1261 cgggagtgat cgtccatgac acgcgtcaag cggggagtgg tggtccgccg ccggcaccga
     1321 aaggtgctga ggctggcccg cggatactgg ggcaagaaga gccgctggtt caaactggcc
     1381 agccagacgg tgaagcgcgc gcttgcccag gcctacgcgc accggcgggc gcggaaacgg
     1441 gagtttcgcc agttgtggat cgcgcgcatc aacgcggcgg cgcgcacgca tgggctctcc
     1501 tatagccggc tgatcttcgg gctccgccgg gcagggatcg aagtgaaccg caagatgctc
     1561 gccgatctgg ctgtggagga cggccaggcg ttctcggcgc tggtcaagcg ggcccagcga
     1621 accgagtagc ccgcggccgg atggtgcgtc agcgcgagcc ttccccgccc ggggagggct
     1681 cgcatcgttc ggtggccggg gatgtcctgg agaccatcat caccagccgc aagaacccgg
     1741 tgatcgagga cctccgctcc ctgctgcgtt cctcgacgcg gcgcaccgcg cggtgcgtcg
     1801 tggaagggtg gcattttctc gaaaccgccg ccgcccgcgg ggcggaatgc cttctcgtcg
     1861 tccacacccc cgccgccgcc gcagacccgc gcgccgggga gctgcgccag cgcctgcatg
     1921 ccgggggcgc caggctggtc gtggtgtccc cgtatgtctt cgaagcgctg acgcaggtgg
     1981 aaacccccca gggggtgctc ggcgtgctcc gacgccctcc ggcggcctcg ccgacggtgc
     2041 tccgcgatcc gcgcttgctg ctcgtggtgc tcgacggcgt acaggatccc ggcaacgtgg
     2101 gggccgtgtt gcgcaccgcc gccgcggccg
//
LOCUS       DASQGH010000247         6419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49233, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000247 DASQGH010000000
VERSION     DASQGH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49233"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..682)
                     /locus_tag="VEW91_04735"
     CDS             complement(113..682)
                     /locus_tag="VEW91_04735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYM90923.1"
                     /translation="MNASLPPQVTGARPALASLAPLAILLGCVGLAARPTPLPAAVVV
                     FVLVGGIGALIPLPERGAPSHRLGVVGRAGVVAFGVATFALARVAAPPLPQPATFLAG
                     AATIGAACAEEIFFRRLVYGFLAQWGDALAVIGAAVAFGAVHIPAYGVQALPVDLAAG
                     LLLGWQRWATGGWGAPAVTHAAANVLQLL"
     gene            complement(720..1472)
                     /locus_tag="VEW91_04740"
     CDS             complement(720..1472)
                     /locus_tag="VEW91_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90924.1"
                     /translation="MRRAWPHRGDRRVLLWGLGAAAVYLAVAAVSLRGGWIPGRVLYD
                     GVAPPPPYRWVHPPPELAKDNQAPDPGTRTIPVTPTGSPPASVSTGDGQAGVDFPQGG
                     IASERGAAAVEVRIVPLDPDTVGGAPPGLRFDSNAYRVDVRDARSGAPARLRAPASIV
                     LRYATGAKGMVRAEGSTWIPLPTTQYGGFLQLVVANTQQVGVFAAVAPRSLPYVERTT
                     RWLYLRILGAALIVGLAIRFLPQILATLRRAR"
     gene            complement(1469..2701)
                     /locus_tag="VEW91_04745"
     CDS             complement(1469..2701)
                     /locus_tag="VEW91_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM90925.1"
                     /translation="MRRPLIVALGLMLAGVTIASVAAPPIVIGAVYPTGGSQGSGGLE
                     ELHGVELAAAYVNQQGGVHGRPIRLQLADAESWDAAPGAVEQLAHSGITVVVGSYGST
                     ISRPAADTASRLGLVFWETGAVGQLGMEAQASPRVFRVSPTGGALGRTAVAFVRDKLA
                     PRLTRPGPLRYAVAYVDDVYGRAVGLGALAELRRSGLPLAGVFPYLLRGLRYPDLAHR
                     IADARPDVLVVGAYLEDGVELRRALVHARVPLVANIGTSSSYCMPAFGQLLGQAAVGV
                     FASDKPEGDVLWPDRLDPAAGRALRWATTESHRRYGEALSAPALAGFAGGIALFQHVL
                     PRARNSSPDAVAEAAREVTLPEGALPNGSGLAYGPPGTQEASTNLRATSVIWEWVGLN
                     TRAVVWPPVFATHPIAFP"
     gene            complement(2740..3198)
                     /locus_tag="VEW91_04750"
     CDS             complement(2740..3198)
                     /locus_tag="VEW91_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90926.1"
                     /translation="MSGQNHSGMRRGGLRARWPAAALLLVTLPLWLGCSARQQTAAPQ
                     PATQVRPRSTATLTFLSPAPGEVVSGPIFHVRLLLTGAEVVPQTSLQLTPDRGHVHLS
                     VDGKVVSMAYGLDQDVQVAPGLHLLQAEFVATDHFPFNPRVLTTITFTAK"
     gene            complement(3224..5173)
                     /locus_tag="VEW91_04755"
     CDS             complement(3224..5173)
                     /locus_tag="VEW91_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HYM90927.1"
                     /translation="MRRGPALTVAALVVMALAAPAGAHALLRSAEPGPGAVLQRAPHD
                     VTITFTEQPEPKLSIIRVLDSAGRPVDQGRAQAVPGHPLELAVPVGTLPRGTYTVSWR
                     TVSQVDGHVTGGAFAFGVGIAPAPTEQPRVTASPPSPAAVAARWAMYVGLCGLMGGGW
                     VWALALPVPAARSGGYLWAAWGTAVAGVIGLGVAQASDAGVGFGQLLDTALGRALLWR
                     ALPLAAAGLAIAAAAPPMPRRRIGMAAVGLAAAASVLAHVLAGHAGAGAPPWRWPNVA
                     VQWAHVCAVGLWLGGLAALLFAIRGAPDAVKAAAVRRFSSVAGIAIAVVAATGVLRSI
                     DEVSSWNALLATEFGHFVLFKSALLLILAALGACNRYWNVPRAMRSLAGLRRVGGAEL
                     AVACVVFVLAGVLTGLAPPSLSGQAAPASQPLVASGSDFATSVRVRLEIAPGFPGPNR
                     FAARIADYDTGRPVAADRVTLWFTKPDRPDIGQSTLTLGRTADGAYAGEGTNLSLSGP
                     WAVAAVIERGPNSVEVRLAVATRIRPLPVHTIEAPGQPTLYGIDLPAGRVLNVYLDPG
                     RGGFNEVHATFIDASGQELPVPRPISITAGRPGAAGQALPVRRFGPGHFIGDAQLEVG
                     EWHLEFAGADAGGTPLEADLTVRVR"
     gene            complement(5298..5786)
                     /locus_tag="VEW91_04760"
     CDS             complement(5298..5786)
                     /locus_tag="VEW91_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90928.1"
                     /translation="MTAVPSSQNRVRRLGAAAGRLFLLATVGVGVALTALDPLAAHHT
                     PWHDHIVLGAHDLKEWAHAVAAHRHLDEGWGALGETRAVPQSPAGIAPNGRPRVLSIA
                     RVPDGAGASILDLNGQVPAGFGAIGQLVLPPRWERGLPLVAQALRPTILPAPDPPPRV
                     SS"
     gene            5916..>6419
                     /locus_tag="VEW91_04765"
     CDS             5916..>6419
                     /locus_tag="VEW91_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYM90929.1"
                     /translation="MPTTDWSSALARAIVANGYSTVAYVPDEVADALLQLLHRDRSMT
                     MVSATREEEALAVVAGAYLGGKRGVLVMQSSGVGNSINALCGLAIGYQIPFLMVISER
                     GRLGEFNPVQMPFGRALPKIFEALGLQAYWLDDAGRIGDVVEGATKLAFSSSLPVAIV
                     LPTMLTGG"
BASE COUNT         1002 a         2321 c         2317 g          779 t
ORIGIN      
        1 aggcgcaccg ggacgcgcgg ccagacgtac gcgcccgcag cgagatacag ggcggcggcg
       61 acagcccccc agacgagcat ccagccgctt gcctgtctcc ttccgctcat gcctacagga
      121 gctgcaggac gtttgcggcc gcgtgcgtca ccgccggggc accccaccct ccggttgccc
      181 agcgctgcca ccccagcagc aaccccgcag cgagatcaac agggagggcc tgcaccccat
      241 acgccgggat gtgcaccgcg ccaaaggcga cggcggcgcc gatgaccgcg agtgcgtccc
      301 cccactgggc caggaacccg tagaccagcc gccggaaaaa gatctcctcc gcacaggcgg
      361 cgccgatcgt cgcggcgccg gcgaggaagg tggccggttg gggaagcggc ggcgccgcca
      421 cacgggcgag ggcgaaggtg gccaccccga acgccaccac gccggcccgg ccaactacac
      481 cgaggcggtg ggagggagcc ccccgctccg gcagcgggat cagcgcgccg atcccgccga
      541 ccaggacgaa cacgacaacc gcggcgggca gcggggtcgg ccgcgcagcg agccccacgc
      601 accccaaaag gatcgcgagc ggcgccaggg acgcaagcgc cggcctggcc ccagtaacct
      661 gtgggggtag ggaggcgttc atcggcgacg aatgtctgat cggagagaag atactgcggt
      721 caccgggccc ggcgaagggt ggccaggatc tgcggcagga agcggatcgc taacccaacg
      781 atcagggccg cgcccaggat gcggaggtag agccaccggg tcgtccgctc gacgtagggg
      841 aggctccgcg gcgcgaccgc ggcaaagacc ccgacctgct gcgtgtttgc cacgacgagc
      901 tgaaggaatc cgccatattg ggtggtcggg agcgggatcc acgtggatcc ttccgcccgg
      961 accattcctt tcgcaccggt cgcatagcga agcacaatgc tcgccggcgc gcgcagcctg
     1021 gccggggcgc ccgagcgcgc atcgcgcacg tccactcggt aggcgttgct gtcgaagcga
     1081 agacccggcg gcgcaccccc caccgtgtct gggtcgagcg gaacgatccg gacctccacc
     1141 gccgcggcgc cgcgttcgga tgcgattccg ccttggggga agtcgacgcc cgcctgcccg
     1201 tcgcccgtcg aaacggacgc gggcggcgac ccggtggggg tcacggggat cgtccgcgta
     1261 ccagggtcgg gtgcctggtt gtccttcgcc agctccggcg ggggatgcac ccaccggtac
     1321 ggaggcggcg gcgccacgcc atcgtacagg acgcgtcccg ggatccaccc accacggagg
     1381 gagacggccg cgaccgccag gtagaccgcg gcagctccca gcccccacag caggacgcgc
     1441 ctgtcgcccc ggtgcggcca tgcacgcctc acggaaacgc gatgggatgg gtggcgaaga
     1501 ccggtggcca gaccaccgcc cgcgtgttga gcccgaccca ctcccagatc acgctggtcg
     1561 cgcgcaggtt cgtgctcgcc tcctgcgtgc cgggcgggcc gtacgcgagg ccgctgccgt
     1621 tcggcagcgc gccctcgggc aaggtcacct cccgggccgc ctcggccacc gcgtctgggg
     1681 aggagttccg cgcccggggg aggacgtgct ggaacagcgc gatgccgccg gcaaacccgg
     1741 ccagggccgg ggcgctgagg gcttccccgt accgcctatg ggactcggtg gtggcccagc
     1801 gcagcgcccg ccccgccgcg ggatccaggc ggtccggcca gaggacgtct ccttcgggtt
     1861 tgtccgaggc gaagaccccg acggcggcct gccccagcag ctggccgaac gcgggcatgc
     1921 agtagctcga actcgtgccg atattggcga cgagggggac gcgggcgtga acaagcgccc
     1981 tgcggagttc gaccccgtcc tcgaggtacg cgcccacgac gagcacatcc ggccgcgcat
     2041 ccgcaatccg gtgtgcgaga tcagggtagc gtaacccccg cagcaggtag ggaaagaccc
     2101 ccgccagcgg gagcccggag cgccggagtt ccgcgagcgc ccccaacccg acggcccggc
     2161 cgtagacgtc gtccacgtag gcgacggcgt accggagggg acccggccgg gtgagccggg
     2221 gcgcgagttt gtcgcgcacg aacgcgacgg ccgtccggcc cagcgccccg cccgtcgggg
     2281 agactcgaaa cacgcggggg ctggcttggg cctccatgcc aagctggccc accgcccccg
     2341 tttcccagaa taccaacccc aggcgcgaag ccgtgtcggc ggccggccgg gagatcgtgc
     2401 tcccgtagct gcccacgacg acggtgatcc ccgaatgcgc cagctgctcc accgcgccgg
     2461 gggccgcgtc ccacgactcc gcatccgcaa gctgcaggcg gatggggcgg ccgtgcacgc
     2521 cgccctgctg gttcacatac gcggcggcca gctccacccc gtgcaactcc tcgaggccgc
     2581 ccgatccctg gctccccccg gtcggataca ccgctccgat cacgatcggc ggcgcagcca
     2641 cggatgcgat cgttactccg gcgagcatca ggcccagcgc cacgatcagt ggtcgccgca
     2701 tcgtccatca cctcattgga aggaatttct cacagccgct cacttagccg tgaacgtaat
     2761 ggtggtgagc acccgcgggt tgaacgggaa gtggtcggtc gcgacgaact ccgcctggag
     2821 gaggtggagg cccggggcga cctggacgtc ctggtccagg ccgtaggcca tggagaccac
     2881 cttcccgtcg acgctgaggt ggacgtgccc ccgatcgggg gtgagctgca gcgacgtctg
     2941 cggcaccacc tcggcgccgg tcagcagaag ccggacgtgg aagatgggcc ccgacaccac
     3001 ctcgcccggg gcgggcgaga ggaaggtcag ggtggcggtc gagcgcggac gcacctgcgt
     3061 ggcgggttgt ggtgcggccg tctgctggcg cgcggagcag cccagccaga gcggaagcgt
     3121 cacgagcaag agcgctgccg ccggccatct cgcgcgtaac cctccgcgtc gcatgccgga
     3181 gtgattctgc ccgctcacag actcctccct gcatcgccgt ggctcatcgc acacgcacgg
     3241 tgaggtcggc ctccaggggc gtgccccctg cgtccgcgcc ggcgaactcg aggtgccact
     3301 cccctacctc cagctgcgca tcgccgatga agtgcccggg gccgaacctg cgcacgggca
     3361 gcgcctgccc cgccgcgccg gggcggcccg cggtgatcga gatggggcgc gggacgggaa
     3421 gctcctgtcc ggacgcgtcg ataaacgtgg cgtgcacctc gttgaatccg ccgcgtccgg
     3481 ggtcgaggta gacgttcagc acccggccgg cgggaagatc gatgccgtac agggtgggct
     3541 ggcccggcgc ctcgatcgtg tgcaccggaa gcggccggat ccgggtcgcc acggccagcc
     3601 ggacctcgac cgagttcggt ccccgttcga tcacggcagc gacggcccac ggtccgctga
     3661 gggagagatt cgtcccttcg cccgcgtacg ccccatccgc cgtccggccg agggtgagcg
     3721 tggactggcc gatgtcgggc cggtccggct tggtgaacca cagcgtcacg cggtccgcgg
     3781 cgaccgggcg tcccgtgtcg tagtcggcga tgcgcgccgc gaagcggttg gggccgggga
     3841 atcccggcgc gatctccaat cggacgcgaa cggaggtggc gaagtcgctg ccggaggcca
     3901 cgagcggttg gctcgccggt gcggcctgcc cggacaggct gggcggcgcc agcccggtca
     3961 gcactccggc gaggacaaag accacgcacg ccacggcaag ttccgccccg cccacccggc
     4021 ggagtccggc caggctccgc atcgcccggg gcacgttcca gtaccggttg caggcgccca
     4081 gcgccgccag gatcagcaga agcgccgact tgaacaggac gaagtgtccg aactccgtcg
     4141 cgaggagcgc gttccagctg ctcacctcat cgatgctgcg gagcacgccc gtggcggcga
     4201 cgaccgcgat ggcgatcccg gcaaccgagg aaaaccgccg gacggcggcg gccttcaccg
     4261 catccggcgc tccccggatg gcgaacagca gcgccgccag cccccccaac cagagcccca
     4321 ccgcgcacac gtgagcccac tgaaccgcca cgttgggcca ccgccagggg ggcgcgcccg
     4381 cccccgcgtg tcccgccagc acgtgcgcca gcacggaggc cgcggcagcc agacccaccg
     4441 cggccatccc gatcctccgg cggggcatcg ggggggccgc cgcggcgatc gccagtccgg
     4501 ctgcggcaag cggcagggcc cgccagagga gcgcgcgccc gagcgccgtg tccagcaatt
     4561 ggccgaaccc cacgcccgcg tcggacgcct gggcgacgcc cagcccgatc acgcccgcga
     4621 ccgcggtccc ccatgcagcc cagaggtagc ccccgctgcg cgcggcgggc accggaagcg
     4681 cgagggccca cacccacccg cctcccatga gcccgcacag tcccacgtac atcgcccagc
     4741 gggcggccac cgctgccggc gacggaggcg acgccgtgac ccggggttgc tcggtggggg
     4801 cgggtgcgat ccccacgccg aacgcgaacg cgccgccggt cacgtgcccg tcgacctggg
     4861 aaacggtccg ccacgatacg gtgtacgtcc cccgggggag ggtgccgacc gggaccgcga
     4921 gctcaagcgg gtgcccgggc accgcttgtg cgcgcccctg atccacgggg cgcccggcgc
     4981 tgtcgagcac ccggatgatc gacagtttgg gctccggttg ctctgtgaac gtgatcgtca
     5041 catcatgcgg ggcacgctga aggacggctc cgggcccggg ctccgcactg cggaggaggg
     5101 catgggcgcc cgcaggggcg gcgagggcca tcaccaccag ggcggcgacg gtgagagccg
     5161 gaccccggcg catgttaccc ggtaccctgc gcgccggccg gggcaacccg cgcggcgtcc
     5221 ggcagcgacg cgacagcaag gccgcagatc tcatccacgc acatctcctc cttcccgggc
     5281 ccagcccgag gtggcggtca ggaggagacg cggggaggag gatctggggc agggaggatc
     5341 gttgggcgca acgcctgcgc gacgagcggg agccctcgtt cccagcgcgg cgggaggact
     5401 agctgtccga tcgccccgaa gccggccggg acctggccgt tcaagtccag gatcgacgcg
     5461 ccggccccat cggggaccct cgcgatagac aggacgcggg gcctgccgtt cggcgcgatc
     5521 cctgcggggg attgggggac tgcccgggtc tcgccgagcg ccccccatcc ttcatcgaga
     5581 tggcgatggg ccgccacggc atgcgcccac tccttgaggt cgtgcgcgcc aaggacgatg
     5641 tggtcatgcc acggcgtgtg gtgtgccgcg agcggatcga gggcggtgag cgcgactccg
     5701 accccgacag tcgcgaggag aaacagacgt cccgcggctg cgcccagccg ccgaacccgg
     5761 ttctgtgaac tcggcacagc cgtcacctgc tgctcatgcg gcgctgcccg tcagtataac
     5821 acaccctgct cggacccgga gcgcaggggt ccttccgggc ggcatcgaaa cgggcggcgg
     5881 gcacgtacgc cggatgagag gggggcacac gcgaggtgcc gacgacggac tggagttccg
     5941 ccctggctcg cgcgatcgtc gccaacgggt acagcacggt cgcgtacgtg ccggacgagg
     6001 tggcggatgc cctcctgcag ctcctgcacc gcgaccgatc gatgaccatg gtctctgcga
     6061 cccgggagga ggaggctctg gccgtggtcg ccggcgcgta cctgggaggc aagcggggcg
     6121 tcctggtgat gcagagcagt ggggtgggga actccatcaa tgcgctttgc gggctcgcga
     6181 tcgggtacca gattccgttc ttgatggtca tcagcgagcg cggacgtctg ggagagttca
     6241 acccggtcca gatgccgttc gggcgggcgc tcccgaagat cttcgaagcg ctcgggctgc
     6301 aggcctactg gctggacgac gccggccgga tcggggacgt tgtcgagggg gcgaccaagc
     6361 tggcgttctc gagctcgctg cctgtggcga ttgtcctccc gaccatgctg acaggggga
//
LOCUS       DASQGH010000248         7199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9525, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000248 DASQGH010000000
VERSION     DASQGH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9525"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="rplF"
                     /locus_tag="VEW91_04770"
     CDS             complement(<1..264)
                     /gene="rplF"
                     /locus_tag="VEW91_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HYM90930.1"
                     /translation="MSRVGRLPIPIPQGVEVAIADQVVRVKGPKGTLERTVHPAVRVA
                     LQDGRIVVTRQSDDRLHRALHGLTRALVANMVHGVVKGYRVELE"
     gene            complement(298..690)
                     /gene="rpsH"
                     /locus_tag="VEW91_04775"
     CDS             complement(298..690)
                     /gene="rpsH"
                     /locus_tag="VEW91_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HYM90931.1"
                     /translation="MITDPIADMLTRIRNALVARSPQVTMPTSRMKLEIAKILKAEGF
                     IADYQMDKARPEVIRITLRYGERKEGIILGLRRISRPGLRIYAQRAELPRVQGGLGVA
                     ILSTSKGVMTDRQARRAGIGGEVLCFVW"
     gene            complement(706..891)
                     /locus_tag="VEW91_04780"
     CDS             complement(706..891)
                     /locus_tag="VEW91_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HYM90932.1"
                     /translation="MPKKALLIKWRRPPKFKSRRFSRCRSCGRPRAVFRKFGLCRICL
                     RTLAHRGEIPGLVKASW"
     gene            complement(898..1443)
                     /gene="rplE"
                     /locus_tag="VEW91_04785"
     CDS             complement(898..1443)
                     /gene="rplE"
                     /locus_tag="VEW91_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HYM90933.1"
                     /translation="MARLRERYKHEVVADLRKRMGYTNVMQVPRVEKIVINMRVKEAL
                     QDQRHLDKAVEELTLISGQRPVITRARRSIAAFKLRTGNPIGCKVTLRGERMYEFLDK
                     LFNISLPRIKDFKGISTRSFDGRGGMNIGIREQLVFPEIEYDKVDKIRGMDITIVTTA
                     RTDEEARELLRSLGLPIREGA"
     gene            complement(1462..1791)
                     /gene="rplX"
                     /locus_tag="VEW91_04790"
     CDS             complement(1462..1791)
                     /gene="rplX"
                     /locus_tag="VEW91_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HYM90934.1"
                     /translation="MRARVHVRRGDTVEVITGKHRGKRGKVLRVFPEDGRIIAEGVMV
                     AKRHMKPTQKMPAGGIVEKEMAFPAGKAMLVCTRCGRAVRFGHRILEDGTKVRICRHC
                     GEIIDKA"
     gene            complement(1791..2159)
                     /gene="rplN"
                     /locus_tag="VEW91_04795"
     CDS             complement(1791..2159)
                     /gene="rplN"
                     /locus_tag="VEW91_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYM90935.1"
                     /translation="MIQNYTRLRVADNTGARQIMCIQVHGGSRRRYAGIGDVIVATVK
                     QAIPNSTVKKGEIVHAVVVRTSQPVRRPDGSYIRFDDNAAVLITDQNNPRGTRIFGPV
                     ARELREKQFMKIISLAPEVL"
     gene            complement(2156..2422)
                     /gene="rpsQ"
                     /locus_tag="VEW91_04800"
     CDS             complement(2156..2422)
                     /gene="rpsQ"
                     /locus_tag="VEW91_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYM90936.1"
                     /translation="MTPPAPRPARKTQVGIIVSDKMQKTVVVEVQTRGRHPLYKKVLT
                     HTRRFKAHDEAGEAKLGDTVLIASTRPLSKEKRWRVARVLERAR"
     gene            complement(2419..2625)
                     /gene="rpmC"
                     /locus_tag="VEW91_04805"
     CDS             complement(2419..2625)
                     /gene="rpmC"
                     /locus_tag="VEW91_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HYM90937.1"
                     /translation="MSAEELRELGLPDLHKRLEEARQELFNLRLQRASGKLLNPARVV
                     LVRRNVARLLTVLRERAGAEGRTP"
     gene            complement(2622..3041)
                     /gene="rplP"
                     /locus_tag="VEW91_04810"
     CDS             complement(2622..3041)
                     /gene="rplP"
                     /locus_tag="VEW91_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HYM90938.1"
                     /translation="MLMPKRVKYRKAHRGRMRGEAHNGASVVFGEFGLQALVRAWVTA
                     QQIEASRRAVTRFIKRGGKLWIRVFPDKPVTKKPAETRMGSGKGNPELWVAVVRPGRV
                     MFELGGVSEAVAREALTLAGHKLPIKTRFVQRAEVQA"
     gene            complement(3010..3765)
                     /gene="rpsC"
                     /locus_tag="VEW91_04815"
     CDS             complement(3010..3765)
                     /gene="rpsC"
                     /locus_tag="VEW91_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HYM90939.1"
                     /translation="MGQKIHPIGLRLGIIKDWESKWYAEKNFPDLIQEDQALRRHIKK
                     KLARAGIARIEIERTANRVRITIHSARPGIIIGRGGTGIDALKKELDALTGKQIQLNV
                     QEIRRAELEAILVAQNVASQLERRVAYRRAMKQAVARTLRSGAKGIRISCSGRLAGAE
                     IARYEWYRDGRVPLQTLRADIDFGVAEALTTYGRIGVKVWIYRGDVLPERRRPGELRG
                     RPGETVVLPSRMPRVTVARRSEASVDAQEGQVP"
     gene            complement(3778..4110)
                     /gene="rplV"
                     /locus_tag="VEW91_04820"
     CDS             complement(3778..4110)
                     /gene="rplV"
                     /locus_tag="VEW91_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HYM90940.1"
                     /translation="MEVRAVARYVRISPRKVRPVMALIKGKGIDEALAVLRFSTNHAS
                     RAVAKVVKSAAANAENNLELAHDTLRVSQAFVDSGPTVRRMQARARGRADVIKKRSSH
                     ITIVVAGE"
     gene            complement(4184..4453)
                     /gene="rpsS"
                     /locus_tag="VEW91_04825"
                     /pseudo
     CDS             complement(4184..4453)
                     /gene="rpsS"
                     /locus_tag="VEW91_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(4497..5321)
                     /gene="rplB"
                     /locus_tag="VEW91_04830"
     CDS             complement(4497..5321)
                     /gene="rplB"
                     /locus_tag="VEW91_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYM90941.1"
                     /translation="MGVKKFKPVTPGRRFMTVSTFDEITSTEPERGLLRPHPSSGGRN
                     AQGRLTVRHRGGGHKRLYRMLDFKRNKDGINARVASIEYDPNRSARIALLHYQDGEKR
                     YILAPVGLGVGDVVESGPKAEVRPGNALPLRVMPLGTTVHNVELQVGRGGQLVRSAGA
                     AAQLMAKEGEYAQVRLPSGEVRKVHLDCRATVGQVGNLEHEALSIGKAGRSRWMGWRP
                     AVRGVVMDPSSHPHGGGEGKSPIGMPGPVTPWGKPTLGYKTRKPKPSDRFIVKRRK"
     gene            complement(5335..5622)
                     /gene="rplW"
                     /locus_tag="VEW91_04835"
     CDS             complement(5335..5622)
                     /gene="rplW"
                     /locus_tag="VEW91_04835"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HYM90942.1"
                     /translation="MTDPRTVIRRPIVTEKSMRGTTIGKYTFEVDRTSPKPVIRDAVQ
                     RLFRVRVTKVNVLTIHGRQRRRGQHRYQESAVRKAIVTLAAGDKIDIEKLG"
     gene            complement(5619..6254)
                     /gene="rplD"
                     /locus_tag="VEW91_04840"
     CDS             complement(5619..6254)
                     /gene="rplD"
                     /locus_tag="VEW91_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HYM90943.1"
                     /translation="MSMVAVYDTEGKRIGEVAVPPSLEHKPHTAVLHEALLWQLAGRR
                     RGTHSTLTRGMVNRSTRKLYRQKGTGRARHGARGAPLFRGGGIIFGPHPRSYAYRLPK
                     QVRRLALRSALAAKVAAGRFAVLDRLDLTEPKTRVLAGLVRGLGGERGTRGTVLLITA
                     APAPIVVRSAANLPQLRVLPATALNLHAILACDQVVVTQDALARITEAFAP"
     gene            complement(6251..6883)
                     /gene="rplC"
                     /locus_tag="VEW91_04845"
     CDS             complement(6251..6883)
                     /gene="rplC"
                     /locus_tag="VEW91_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HYM90944.1"
                     /translation="MAALLGKKLGMTQVFDDAGNVVPVTVVEAGPCVVVGVRTQAREG
                     YAAVQLGFGEVAEAKIPKPLRGVFTRRGLRPHRVIREVPLAAGEQLEVGQSVTAAVFA
                     RGDRVDVVGLTKGKGFAGTIKRHNFGGQRDSHGVSLMHRAVGSIGSSNIARVMKGKRM
                     PGRLGGTRVTIRNLDVVSVDPERNVLLLRGAVPGPQGALVVIRKVGTAGK"
     gene            complement(6903..>7199)
                     /gene="rpsJ"
                     /locus_tag="VEW91_04850"
     CDS             complement(6903..>7199)
                     /gene="rpsJ"
                     /locus_tag="VEW91_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HYM90945.1"
                     /translation="KIRIKLKAYDHKVLDQSAEKIVDTVKRTGARISGPVPLPIDRNI
                     YCVIRSPHIDKESMEHFELRTHKRLIDILEPTPKTVDALMHLDLPAGVDIEIKL"
BASE COUNT          941 a         2726 c         2301 g         1231 t
ORIGIN      
        1 ctccaattcc acccggtacc ctttcaccac cccgtggacc atgttggcga ccagcgcccg
       61 ggtgagcccg tgcagcgccc ggtgcagccg gtcatcgctc tgccgggtca cgacgatccg
      121 gccgtcctgg agcgccaccc ggaccgccgg gtgcacggtc cgctccaacg tccccttggg
      181 gcccttgacg cggaccacct ggtcggcgat cgcgacctcg accccctgcg gaatgggaat
      241 gggcaaccta ccgactcggg acatcgccct cccctccctg ccccggcctc ccggggccta
      301 ccagacgaag cacagcacct cgcccccgat ccccgcccgg cgcgcctgcc ggtcggtcat
      361 cacgcccttg ctggtcgaca ggatggccac gccgaggccg ccctggaccc ggggcaactc
      421 ggcgcgctgc gcgtagatcc tgaggcccgg ccggctgatg cgccgcaggc cgaggatgat
      481 gccctctttg cgctcgccgt accggagggt gatccggatc acctcggggc gggccttgtc
      541 catctggtag tccgcgatga acccctcggc cttcaagatc ttcgcgatct cgagcttcat
      601 ccgcgaggtc ggcatcgtga cctggggact ccgggccacg agcgcgttgc ggatgcgggt
      661 cagcatgtcc gcgatgggat ccgtgatcac acccactggg tcacctcacc aactggcctt
      721 caccaggccg ggaatctccc cgcggtgcgc cagcgtccgc aggcagatcc tgcacagccc
      781 gaatttccgg aacaccgccc gcggccggcc gcacgaccgg caccggctga accgccggga
      841 cttgaacttc ggcggcctcc gccatttgat cagcagcgcc ttcttgggca tcccccgcta
      901 cgccccctcg cggatcggca agccgagcga gcgcagcagc tcgcgcgctt cctcgtccgt
      961 ccgcgccgtc gtgacgatcg tgatatccat cccgcggatc ttgtcgacct tgtcgtactc
     1021 gatctccggg aagaccagct gctcccgaat cccgatgttc atgccccccc ggccgtcgaa
     1081 cgagcgcgtc gagatgccct tgaagtcctt gatgcggggc aggctgatgt tgaagagctt
     1141 gtccaggaac tcgtacatgc gctcgccgcg cagggtcacc ttgcagccga tcgggttccc
     1201 cgtccggagt ttgaacgccg cgatcgaccg gcgggcccgg gtgatcaccg gccgctgccc
     1261 gctgatgagc gtcagctcct cgaccgcctt gtccaggtgc cgctggtcct ggagcgcctc
     1321 cttgacccgc atgttgatca cgatcttctc cacccggggc acctgcatga cgttggtgta
     1381 gcccatgcgc ttgcgcaggt cggccacgac ctcgtgcttg tatcgctcgc gtaatctcgc
     1441 catcgtcgct cgctcgcttc gctacgcctt gtcgatgatc tcgccgcagt ggcggcagat
     1501 ccggaccttc gtcccatcct ccaggatccg gtgcccgaac cggacagccc ggccgcagcg
     1561 ggtgcacacc agcatggcct tcccggccgg aaacgccatc tctttctcca cgatgccccc
     1621 cgccggcatc ttctgggtgg gcttcatatg gcgcttcgcc accatgaccc cttccgcgat
     1681 gatccggccg tcctccggga acacccgcag caccttgccg cgcttccccc ggtgcttgcc
     1741 cgtgatcacc tcgaccgtgt ccccgcgtct cacatggacc cgcgcgcgca tcacagcacc
     1801 tccggggcga gcgagatgat cttcatgaac tgcttctccc tgagctcgcg cgccacgggg
     1861 ccgaagatgc gggtcccgcg ggggttgttc tggtcggtga tcagcaccgc ggcattgtca
     1921 tcgaaccgga tgtacgagcc gtcgggccgc cgcaccggct ggctggtgcg gaccaccacc
     1981 gcgtgcacga tctcgccctt cttgacggtg ctgttgggga tcgcctgctt gaccgtcgcg
     2041 acgatcacat cgccgatccc cgcgtaccgg cgccgggagc ccccgtgcac ctggatgcac
     2101 atgatctgcc gggcgcccgt attgtcggcc acgcggagcc gggtgtagtt ctggatcatc
     2161 gcgcccgctc caagacccgc gccacccgcc accgcttctc cttgctcagc ggccgggtcg
     2221 aggcgatcag caccgtgtcg cccaatttcg cctcccccgc ctcatcgtgg gccttgaacc
     2281 ggcgggtgtg cgtcaggacc ttcttgtaca gggggtgccg gccccgggtc tggacctcga
     2341 ccacgaccgt cttctgcatc ttgtcgctga cgatgatgcc gacctgggtc ttccgggccg
     2401 ggcgcggtgc gggtggggtc acggcgtcct cccttcggcc ccggcgcgtt cgcggagcac
     2461 cgtgaggagg cgggcgacat tccggcgcac gagcaccacg cgggccgggt tcaagagctt
     2521 gccggacgcc cgctggaggc gcagattgaa cagttcctgc cgggcctcct cgaggcgctt
     2581 gtggaggtcg ggtagcccca gttcccgcag ctcttccgcg ctcatgcctg gacctcggcg
     2641 cgctgcacga accgcgtctt gatcgggagc ttgtgtccgg ccagcgtcag ggcttcgcgg
     2701 gcgacggcct cactcacccc ccccagttcg aacatcaccc gccccggccg cacgacggcc
     2761 acccacagct ccgggttccc cttcccgctg cccatccggg tctcggccgg cttcttcgtg
     2821 accggtttgt ccggaaacac ccggatccac agcttccccc cgcgcttgat gaaccgcgtc
     2881 accgcccggc gcgaggcctc gatctgctgg gccgtgaccc aggcgcgaac cagggcctgc
     2941 aggccgaact ccccgaacac gaccgaggcg ccgttgtgcg cctcgccccg catccggccc
     3001 cggtgcgcct tacggtactt gaccctcttg ggcatcaaca ctggcttcgg acctcctcgc
     3061 taccgtcacc cgcggcatcc tcgaggggag gaccaccgtc tcccccggcc ggccccggag
     3121 ttcgccgggc cggcggcgct ccggcaggac gtcgccgcgg tagatccaca ccttcacccc
     3181 gatccgcccg taggtcgtga gcgcctcggc caccccgaaa tcgatgtccg cgcggagggt
     3241 ctgcaggggg acgcggccgt cccggtacca ctcgtagcgg gcgatctcgg cgccggcgag
     3301 ccggccgctg cacgaaatcc tgatcccctt ggccccgctc cggagcgtgc gcgccaccgc
     3361 ctgcttcatc gcgcgccggt acgccacccg ccgctccagc tggctcgcga cgttctgggc
     3421 gacgaggatc gcctcgagct cggcccggcg gatctcctgg acgttgagct ggatctgctt
     3481 gccggtgagc gcgtccagct ctttcttgag cgcgtcgatc cccgtcccgc cccgcccgat
     3541 gatgatcccg ggccgggcgg agtggatggt gatgcgcacc cggttggcgg tccgctcgat
     3601 ctcgatccgc gcgatcccgg cgcgggccag cttcttcttg atgtgccggc ggagggcctg
     3661 atcctcctgg atgaggtcgg ggaagttctt ctcggcgtac catttcgact cccaatcctt
     3721 gatgatcccg agccgcaacc cgatcggatg gatcttctgc cccaccgccc cgcctcctta
     3781 ttcgcccgcg accacgatgg tgatgtggct gctccgcttc ttgatgacgt cggcccggcc
     3841 ccgcgcccgc gcctgcatcc gccgcacggt cgggccgctg tcgacaaacg cctgcgagac
     3901 cctaagcgtg tcgtgcgcca gttcgaggtt gttctccgcg ttggcggccg ccgacttgac
     3961 gaccttggcc accgcccggg aggcgtggtt ggtcgagaac cggagcacgg cgagggcctc
     4021 atcgatcccc ttccccttga tgagcgccat cacgggccgg accttccgcg gcgagatgcg
     4081 cacgtaccgc gcgacggccc tcacctccac ggcgatcccc ccctcatgac ggcggcgccg
     4141 gggtgggcgt cgccgccgcc gcgggcgcgc cggggccggc gggcctcacc gtggtcgtct
     4201 tctccacccg cgcgccgtgg ctcttgaacg tccgcgtggg ggagaactcc cccagtttgt
     4261 gccccaccat gttctcgctc acatagaccg gcacgtgctt ccgcccgtcg tagaccgcga
     4321 tcgtgtgccc gaccatctcc ggcacgatcg tcgaccgccg ggaccacgtc cgaatcacgc
     4381 gcttctcccg cgtcttgttg agcccccgga ccttgtccag cagatgcgcg tcgacaaacg
     4441 ggcccttctt gatcgaccgg cccctgcctc agcgccccct ctttggcata cgcatcctac
     4501 tttctccgct tcacgatgaa ccggtcgctc ggcttcggct tccgggtctt gtaccccagg
     4561 gtcggcttcc cccacggggt gaccgggccg ggcatcccga tcggcgactt cccttcgccc
     4621 ccgccgtgcg ggtggctcga cggatccatg accaccccgc gcaccgcggg ccgccacccc
     4681 atccaccgcg agcggccggc cttgccgatg ctgagcgcct catgctcgag gttgccgacc
     4741 tggcccacgg tcgcgcggca gtcgaggtgg accttccgga cctcgcccga ggggagccgg
     4801 acctgggcgt actctccctc cttggccatc agttgggcgg cggcgccggc gctccgcacc
     4861 agttggcccc cccggcccac ctgcagctcc acgttgtgga ccgtggtccc gagcggcatc
     4921 acccgcaggg ggagggcgtt ccccggcctg acctccgcct tgggccccga ctccaccaca
     4981 tcgcccaccc ccaggccgac gggcgccaga atgtagcgct tctcaccgtc ctggtaatgg
     5041 aggagcgcga tacgggcgga ccggttcggg tcgtactcga tgctcgccac ccgggcgttg
     5101 atcccgtcct tgttgcgctt gaagtcgagc atccggtaga ggcgcttgtg gcccccgccc
     5161 cggtgccgga cggtcagccg gccctgagcg tttcgcccgc cggaggacgg gtggggccgg
     5221 agcagccccc gctcgggctc cgtcgaggtg atctcgtcga aggtggagac ggtcatgaac
     5281 cgccgccccg gggtgaccgg cttgaacttt ttcacgccca tgggatcccg cctcctaccc
     5341 gagcttctcg atgtcgattt tgtcgccggc ggcgagggtg acgatcgcct tccggaccgc
     5401 cgactcctgg tagcggtgct ggccccgccg gcgctgccgt ccgtgaatcg tgagcacgtt
     5461 caccttggtc acccggaccc ggaacagccg ctggacggcg tcccggatca ccggcttggg
     5521 cgaggtgcga tccacctcga acgtgtactt gccgatcgtc gtcccgcgca tgctcttctc
     5581 ggtcacgatc ggacgccgga tcacggtccg cggatcggtc atggggcgaa cgcctccgtg
     5641 atccgggcca gcgcgtcctg ggtcaccacc acctgatcgc acgcgaggat ggcgtggagg
     5701 ttgagcgcgg tggccgggag cacccggagc tgcggcaggt tggccgccga gcgcacgacg
     5761 atgggggcgg gcgcggcggt gatcaggagg accgtgccgc gcgtcccgcg ctcccccccg
     5821 agcccccgca ccaggcccgc caggacccgg gtcttgggct cggtcaggtc gaggcggtcg
     5881 aggacggcga accgcccggc ggcgaccttg gccgccagcg ccgaccggag ggccagccgc
     5941 cggacctgct tgggcagccg gtaggcatac gagcgggggt gggggccgaa gatgatcccg
     6001 ccccccctaa agagcggcgc gccgcgcgcg ccgtgccggg cgcggcccgt ccccttctgc
     6061 cggtacaact tgcgggtgct gcggttcacc atcccgcggg tgagggtcga gtgcgtgccc
     6121 cgacggcggc cggcgagctg ccagagcagg gcctcgtgca gcacggccgt gtggggcttg
     6181 tgctcgaggg acggcggcac cgccacctcc ccgatgcgct tcccctccgt gtcgtacacc
     6241 gcgaccatgc tcatttgccc gccgtgccca ccttccggat caccacgagc gcgccctggg
     6301 gtcccgggac cgcgccccgg agcagcagca cgttccgctc ggggtccacc gagaccacgt
     6361 cgaggttgcg gatcgtcacc cgggtcccgc cgagccgccc gggcatgcgc ttccccttca
     6421 tcacccgggc gatgttgctc gatccgatgc tcccgaccgc gcggtgcatc aggctgaccc
     6481 cgtgggagtc ccgctgcccc ccgaagttgt ggcgcttgat cgtccccgcg aaccccttgc
     6541 ccttggtgag ccccacgacg tccacccggt ccccccgggc gaacaccgcg gccgtgaccg
     6601 actgtcccac ctcgagctgc tcccccgcgg cgagcggcac ctcgcggatc acgcggtgcg
     6661 gccgcagccc ccggcgggtg aacacgcccc ggaggggttt ggggatcttc gcctccgcca
     6721 cctcgccaaa cccgagctgc accgccgcgt acccttcgcg cgcctgggtg cgcaccccca
     6781 cgacgacgca gggcccggcc tcgacgacgg tgacgggcac gacgttgccc gcgtcgtcga
     6841 acacctgggt catcccgagt ttctttccca gcaacgcagc catgcggctc gctctcctgt
     6901 gcctacaact tgatctcgat gtccacgccc gccggcaggt cgagatgcat gagggcgtcc
     6961 acggtcttgg gcgtcggctc gaggatatcg atgaggcgct tgtgggtccg gagctcgaaa
     7021 tgctccatgg attccttgtc gatgtggggc gagcggatca cgcagtagat attgcggtcg
     7081 atcgggagcg ggacgggccc cgagatgcgc gcaccggtcc gcttgaccgt gtccacgatc
     7141 ttttccgcgg actggtcgag gaccttgtgg tcgtaggctt tcagtttgat ccggatctt
//
LOCUS       DASQGH010000249         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49236, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000249 DASQGH010000000
VERSION     DASQGH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49236"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="VEW91_04855"
     CDS             complement(<1..1534)
                     /locus_tag="VEW91_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYM90946.1"
                     /translation="MARRRASRPNPAKVVGALLIALALLIGISIVPAQSARFPGVVAA
                     RLGEAVGKGLPPLPILCLLVGLIFFVRESFRLSARVYGILVGYLAGLVVLHLRFPPGR
                     AFAAAARGEGGGYVGAVVVSALRRWIGEPGLWLVVVVAAVAAALLIADRSLTEALRAI
                     ARGVHRVGPMLGAGALRAAAAAGAAARDALRGVPGAVGRGMQALAALARASLAHRQPR
                     PQTEVGFAPGQEAFPEGESASAAAGGSFPGALLGASGRAPREPEDLAPAPPEAAPVRE
                     PSRVGEAGGDLGEPGGGRRRKRAVVEQELLQFPTPPRPGYTLPPPSLLDTDAAGKARG
                     GAEAPEEVARNLERTLASFGVEAKVIRWEIGPVVTRFELQPAPGVKVQRITSLQNDIA
                     LALAASSVRLEAPIPGKAAIGVELPNERPGLVHLGEMLASPELQRSPSPLTVSIGKGI
                     AGAPIIADLVAMPHLLIAGATGAGKSVALNSMIATLLFRATPEQVRFLMIDPKRVELA
                     NYN"
     gene            complement(1573..3345)
                     /locus_tag="VEW91_04860"
     CDS             complement(1573..3345)
                     /locus_tag="VEW91_04860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HYM90947.1"
                     /translation="MTARRRRRRRPAPPVPGGGPLPAPSSAPAAQPPRAPRTLAERKG
                     EGVQIIPLGGLGEIGKNSLAIRLGRDILIVDAGLMFPDEEMLGIDFAIPDFRAIAAEG
                     TVRGIVLTHGHEDHVGGLPYLLQTVQAPVYGTPLTLGLARRRVEETRGAPKMKGVPAE
                     PRRPVRIGPFEVELVHVNHSIPQACAVAVRTPVGTVVCTSDFKFDQTPIGEGPTDFSR
                     LAEIGEAGVLVALMDSTNVERPGYAPSERVVGAALDDIFARSHGRILVTTFASNVHRV
                     QQIFETAGRHDRKVAVAGRSMVDTVAIASELHALKVPRGTLVPLEQIRRLPERHVAIL
                     TSGSQGEPLSALTRMAAGTHRAVTIKSGDTVVFSATPIPGNEAMVWRTINQLYRQGAQ
                     VIHGAHVHASGHACQEELKLMLTLLRPTFFIPVHGEYRHLVLNQRLATSVGIPEERTL
                     LAENADRIDVSAGRMVRAGTIEAGTVLVDGLGIGVGEAVLRDRRHLAQDGVLIALVAL
                     DRQSGDLVREPDIVSRGFVYMRESGDLIEECKKRVREAVIRCRDRGNIEWGAVRSAIR
                     ETLGRYLFDQTHRQPMILPLLVEL"
     gene            complement(3342..3896)
                     /locus_tag="VEW91_04865"
     CDS             complement(3342..3896)
                     /locus_tag="VEW91_04865"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYM90948.1"
                     /translation="MRRCRRCPLWRTRTHAVPGEGDAHRGIMLVGEGPGREEDLAGRP
                     FVGRAGRLLDELLASVGLRREDLYVTNVIKDRATTATPPRRDRPPTPSEISACAPWLA
                     AQREIIRPRIVVTLGRLALAAFLPGALIRDVHGRPQQSGGRTILPLYHPSYALHNPRL
                     RPVLFHDIRALRDLLDRGREARPR"
     gene            complement(3949..4842)
                     /gene="dapA"
                     /locus_tag="VEW91_04870"
     CDS             complement(3949..4842)
                     /gene="dapA"
                     /locus_tag="VEW91_04870"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYM90949.1"
                     /translation="MAGLGHVITAMVTPMDRTLAVDYDRAASLAKRLVESGSDGLVVC
                     GTTGESPTLTDEEKVRLFRTIREAVGPRAKVIAGTGTYDTAHSIHLTREAERAGCDGV
                     LLVNPYYNKPSQEGLYRHFRAVAESTSLPVMLYNIPGRTSVNCEPATIARLAEVPNIV
                     AVKEASGSLDQMSQIRKLTPPEFHLYSGDDSLTLPLLAVGGTGVVSVAAHLAGREIKA
                     MIQAYQAGEVRQALALHLRLWPLFKVLFITTNPTPVKAALALAGFDVGGLRLPLIEAT
                     TKEREQIGAVLKELALIPVAA"
     gene            complement(4921..>5796)
                     /locus_tag="VEW91_04875"
     CDS             complement(4921..>5796)
                     /locus_tag="VEW91_04875"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYM90950.1"
                     /translation="DALRGAQIAFFGGGDGASARFARAAAASGTLVIDKSSTFRLAPD
                     VPLVIPEVNRHALRQHRGIIANPNCSTITMVMALAPLHDLVPVRRVVACTYQSVSGAG
                     RDAMDTLLDQSRRLLDRADLLRDRPEVGEVERVTGTPLPIAFNVLPQWKWMPGGVTEE
                     EDKMVRETHKILEADLGVSVTTVRVPVLIGHLIALHVEFTGPLSVERARAQLAAAPGI
                     EVADDCERGIYPTPLYATGRDACVVGRLRPDPTVPHGMCLCAVTDNLRKGAALNAVQI
                     AEAVVEEGLLAASRV"
BASE COUNT          842 a         2115 c         2024 g          815 t
ORIGIN      
        1 cgttatagtt ggccagctcc acccgctttg gatcgatcat caggaaccgc acctgctcgg
       61 gggtggcgcg gaacaggagc gtggcgatca tggagttcag cgccacgctc ttgccggcgc
      121 cggtggcccc cgcgatgagc aggtgcggca tcgcgacgag atcggcgatg atgggagcgc
      181 cggcgatccc cttcccgatc gagaccgtga gcggggacgg cgaccgctgg agttccgggc
      241 tcgccagcat ctcccccagg tgcaccaggc cgggccgctc gttgggcagc tcgacgccaa
      301 tcgcggcctt ccccgggatc ggggcttcca gccggacgct gctcgccgcg agcgcgagcg
      361 cgatgtcgtt ctggaggctg gtgatccgct ggaccttgac cccgggagcc ggttggagtt
      421 cgaaccgggt gaccacgggg ccgatctccc accggatcac cttcgcctct acgccgaagc
      481 tggccagggt ccgctcgagg ttgcgcgcca cctcctcggg agcctccgcg ccgccccggg
      541 ccttgccggc cgcgtccgtg tccagcaagg atgggggggg aagcgtgtac cccggacgcg
      601 gcggggtggg aaactggaga agttcctgct ccacaacggc ccgcttgcgg cgccgcccgc
      661 caccgggctc tcccaggtcg ccccctgcct cccccacccg cgacggctct cggaccggcg
      721 ccgcctccgg gggagcgggc gccagatcct ccggctcccg gggcgcgcgc cccgaggccc
      781 ccaaaagagc gccggggaaa gacccccccg cagccgcgga ggcggattcg ccctccggga
      841 acgcctcctg gcccggtgca aacccgacct ccgtctgggg acgcggctgc cggtgcgcca
      901 gggaggcacg cgcgagggcg gcgagggcct gcatcccgcg cccgaccgcc ccggggacgc
      961 cccggagcgc gtcccgcgcg gccgcgccgg ccgccgcggc cgcgcggagg gcgcccgctc
     1021 cgagcattgg gccgacgcgg tggacccctc gggcgatcgc gcggagcgcc tcggtgaggg
     1081 accggtctgc gatcagcagc gcggccgcaa ccgccgcgac cacaaccacc agccacaagc
     1141 ccggctcgcc gatccatcgt ctgagcgccg agacgaccac cgccccgacg tagcccccac
     1201 cctctccgcg ggcggccgcg gcgaacgcgc ggcccggcgg gaaccggagg tgcaggacca
     1261 cgaggccggc gaggtagccg accaggatgc cgtacacgcg ggcgctcaga cgaaacgact
     1321 ccctgacgaa gaagatgagg ccgaccagga ggcacaggat cggcagcggg ggcagcccct
     1381 tccccaccgc ctcgcccagc cgggccgcaa ccaccccggg gaagcgggcg ctctgcgcag
     1441 gaacgatgct gatgccgatg agcagggcca gcgcgatgag aagcgcgccg accacctttg
     1501 cgggattggg gcgagacgct ctacgcctgg ccacggtgcg gcctcctctg ctgcgaaccg
     1561 gtgcgacgag tgctacagct ccacgagcaa cggaaggatc atcggttgcc ggtgcgtctg
     1621 gtcgaacaga taccggccaa gcgtctccct aatcgccgag cgcactgccc cccattctat
     1681 gttgccgcgg tcgcggcatc gaatgacggc ctctcgaacg cgcttcttac actcctcgat
     1741 caggtctccc gattcacgca tgtagacgaa gccgcgggag acgatgtccg gctcgcgaac
     1801 caagtccccg ctctggcgat cgagcgccac cagcgcgatc agcacaccgt cctgggccag
     1861 gtggcggcgg tcccgcagca cggcctcgcc caccccgatc cccagcccgt cgaccaacac
     1921 ggtcccggcc tcgatcgtcc ccgcccggac catccggccc gccgagacgt cgatcctatc
     1981 tgcattctct gccagcaggg tgcgttcctc cgggatcccc acgctggtcg cgaggcgctg
     2041 gttaagcacc aggtgccggt actccccgtg gaccgggatg aaaaacgtgg gccgcagcag
     2101 ggtcagcatc agtttcagct cctcctggca ggcgtgcccc gacgcgtgca cgtgggcgcc
     2161 gtggatcacc tgggcgccct gccggtagag ctggttgatc gtgcgccaca ccatggcctc
     2221 gttgccgggg atgggggtgg ccgagaacac caccgtatcg ccggacttga tcgtgaccgc
     2281 ccggtgcgtc cccgccgcca tccgcgtcag cgcagagagc ggttccccct gactgccgct
     2341 ggtcaggatc gccacgtgcc gctcgggaag ccggcgaatc tgctccagcg gcacgagggt
     2401 ccctctcggg accttgagcg cgtgcagctc cgaggcgatc gcgaccgtat cgaccatgct
     2461 ccggcccgca acggcgacct tgcggtcgtg gcgcccggcc gtctcgaaga tctgctggac
     2521 ccggtgcacg ttgctggcaa acgtcgtcac cagaattcgc ccgtgggaac gcgcgaagat
     2581 gtcgtccagc gcggcgccca ccacccgctc ggacggcgcg taccccgggc gttccacatt
     2641 ggtcgagtcc atgagggcga ccaggacccc ggcctccccg atctccgcga ggcgcgagaa
     2701 gtccgtcggg ccctcgccga tcggcgtctg gtcgaacttg aaatcgctcg tgcagaccac
     2761 cgtccccacc ggggtccgca ccgcgaccgc gcacgcctgg gggatgctgt ggttgacatg
     2821 cacaagctcg acctcaaacg gcccgatccg caccgggcgc cggggttcgg cggggacgcc
     2881 cttcatcttc ggggcgccgc gggtttcctc cacccggcgg cgggcgagtc cgagcgtcag
     2941 cggcgtccca tacaccgggg cctggaccgt ctggaggaga tagggcagcc cgccgacgtg
     3001 atcctcgtgc ccgtgcgtga gcacgatccc gcgcaccgtg ccctccgccg cgatcgcacg
     3061 gaagtcgggg atcgcaaaat cgatgcccag catctcctcg tcggggaaca tcaggccggc
     3121 atcgacgatc aggatgtcgc gccccaggcg gatcgcgagg ctgttctttc cgatctcccc
     3181 gagccccccg agcgggatga tctggacccc ctcccccttg cgctccgcca gggtgcgtgg
     3241 cgcacgcggg ggctgcgcgg ccggggcgct cgacggcgcg ggcagcgggc ctccgccggg
     3301 gaccggcgga gcggggcgtc gcctccggcg cctccgggcc gtcaccgggg ccgggcttcc
     3361 cgcccgcgat cgagcagatc ccggagcgcc cggatgtcgt ggaacagcac cgggcggagg
     3421 cgggggttgt ggagcgcgta cgagggatgg tagagcggga ggatcgttcg gccgccgctt
     3481 tgctgtgggc ggccgtgcac atcccggatc agggccccgg ggagaaacgc cgccagcgcc
     3541 aggcggccga gggtgaccac gatgcggggc cggatgatct cccgctgggc ggccagccac
     3601 ggcgcgcacg ccgagatctc cgagggtgtc ggggggcggt cccgccgagg cggtgtcgct
     3661 gtggtggccc ggtccttgat gacgttggtc acgtagaggt cttcccggcg gagcccgacg
     3721 ctggccagga gctcgtccag cagccgcccg gcccggccca caaacggccg gccggcaaga
     3781 tcctcctctc ggcccgggcc ctcgccgacg agcatgatcc ccctgtgggc atccccttct
     3841 ccgggaacgg cgtgggtccg ggtccgccag agcgggcacc gccggcagcg ccggatccgg
     3901 gcgtggagta cctcgagtcc gcgggcgccg tgcgccggga gccctcgcct aggccgcgac
     3961 ggggatcagg gccagttcct tgagcacggc cccgatctgc tctcgctcct tcgtcgtggc
     4021 ttcgatgagg ggaagccgca gcccgccgac gtcgaatccg gcgagggcca gcgccgcctt
     4081 cacgggcgtc ggattggtgg tgatgaacag caccttgaac agcggccaga gccggaggtg
     4141 gagcgcgagg gcctgccgga cctcaccggc ctggtaggcc tggatcatcg ccttgatctc
     4201 gcgcccggcg aggtgggcgg caacgctcac gaccccggtg ccccccacgg cgaggagcgg
     4261 aagggtcagg ctgtcatccc cgctgtacag gtggaactcc gggggcgtga gcttgcggat
     4321 ctgggacatc tggtccaggc tcccgctcgc ctctttgacc gccacgatgt tcgggacctc
     4381 cgccagacgc gcgatcgtcg ccggttcgca gttgaccgac gtgcgacccg ggatgttgta
     4441 cagcatgacc ggaagcgacg tgctctcggc gacggccctg aagtgccggt acaggccttc
     4501 ctgcgaaggc ttgttgtagt aggggttgac gaggagcacg ccgtcacacc ctgcccgctc
     4561 ggcctcgcgc gtcaggtgga tgctgtgggc ggtgtcgtag gtgccggtcc cggcgatgac
     4621 cttcgcgcgc gggcccaccg cctcccggat ggtgcgaaag agccggacct tttcctcgtc
     4681 ggtcaacgtg ggggattccc cggtcgtccc gcagaccacc agcccgtcgg agccggactc
     4741 gacgagccgc ttggccaacg acgccgcccg gtcgtagtcg accgccagcg tgcggtccat
     4801 cggtgtcacc atggcggtga tgacgtgccc gagcccggcc attggtctcc ctccctctcc
     4861 cgtgcctcgc tcctcacctg acatgttcag gattcccggg gcaagctcct gccgccgaga
     4921 tcacacgcgc gaagcggcga gcaggccctc ttccaccacg gcctcggcga tctgcacggc
     4981 gttcagcgcg gcgccctttc gcaggttgtc cgtcacggca cacaggcaca tgccgtgcgg
     5041 gacggtgggg tcaggacgga ggcgaccgac cacgcaggcg tcccgtcccg tcgcgtagag
     5101 cggcgtcggg tagatccccc gctcgcaatc gtcggccacc tcgatgcccg gcgccgccgc
     5161 cagctgcgcc cgggcgcgct caacggaaag cggcccggtg aactccacgt ggagcgcgat
     5221 gaggtggccg atgagcaccg ggacccggac cgtcgtcacg ctcaccccga ggtcagcctc
     5281 aaggatcttg tgcgtctccc ggaccatctt gtcttcctct tccgtgaccc cgcccggcat
     5341 ccacttccac tgcgggagca cgttgaacgc gatcggcagg ggggtgcccg tgacccgctc
     5401 gacctccccg acctccgggc gatcccggag gagatcggcg cgatcgagga gccggcggct
     5461 ctgatccagc agggtgtcca tggcatcccg gccggcgccc gacacggatt gatacgtgca
     5521 ggccacgacc cgtcggaccg gcacgaggtc gtgcaggggc gcaagcgcca tgaccatcgt
     5581 gatcgtggag cagttcgggt tcgcgatgat cccacgatgc tggcgcagcg cgtgccggtt
     5641 gacctccggg atcaccaggg ggacgtccgg cgccaggcgg aacgtcgaac tcttatcgat
     5701 gacgagggtg ccggacgcgg cggcggcgcg ggcaaagcgc gcgctggccc catcccctcc
     5761 cccgaagaac gcgatctggg cgccccgcag cgcgtc
//
LOCUS       DASQGH010000250         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20878, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000250 DASQGH010000000
VERSION     DASQGH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20878"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEW91_04880"
     CDS             <1..424
                     /locus_tag="VEW91_04880"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYM90951.1"
                     /translation="EKSPAAICRKVALARDGGEIEVWGDGKQTRSYCYVDDCVEGIHR
                     LMQTEEHDPINLGTDRMVTIDELVDIIAGIAGKRVIKRYDLTKPQGVRGRNSDNTRLR
                     RVLRWEPRVPLEEGLAKTYRWIREQLIKAGRVPEEALR"
     gene            477..1301
                     /locus_tag="VEW91_04885"
     CDS             477..1301
                     /locus_tag="VEW91_04885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYM90952.1"
                     /translation="MFAGVPVAEVAEYWDRRPCNIRHSPREIGTRAYFDEVEARKSFV
                     EPHALRFAEFPRWAGKRVLEIGCGIGTHTIGFARGGAVVTAMDLSERSLEIARRRAAV
                     YGLDGRITFYAGNAEELDAVVPVEPYDLIFSWGVIHHTPHPGRVIDQIRRYTRPGTTV
                     KLMVYHRRSWKILGIVLRHGRGRFWKLDELVARHSEAETGCPVTYTYRPSDVEALLAG
                     FAIREMWVDHIFPYRVNEYLQYRYVKAWYFHAMPGPMFRWLERHFGWHLCVTAEAR"
     gene            1359..2669
                     /locus_tag="VEW91_04890"
     CDS             1359..2669
                     /locus_tag="VEW91_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HYM90953.1"
                     /translation="MVGLGKLGACTAACLAARGFDVLGIDINPGFVQAINRGIAPVSE
                     PRLQDLITAAGPQIRATHDYAQAIDESDVTFLVVPTPVQPDGVFSDAHLRDALTRLCS
                     PFERSHKPYHLFVVTSTVSPGTTEGQLIPLIESLTGKRLHEHFGVCYNPEFIALGSVI
                     TDFLNPDLVLIGESDRAAGDLLEGIYRQVCENEPVMARMALVSAEITKVSLNSYVTMK
                     ISFANTLASLCEAIPGAEIDLITRALGADRRVSPHTLKGGPPFGGPCFPRDNRAFAAF
                     ARRYGVDAKLATASDEINASQAERLAALVLRQLAEVKDRTVSILGLAYKANTPVVEES
                     PAIQIIHALLERGMDVMVYDPLAMDDTRAVFGERIRYAPSAKACAAYGAVCVVTTQWE
                     EFAGIQAGDFTHNPTVVIDCWRMLDPARLGARVKYVAMGASPRR"
     gene            2673..>2986
                     /locus_tag="VEW91_04895"
     CDS             2673..>2986
                     /locus_tag="VEW91_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90954.1"
                     /translation="MRLEDSGRIRSEARPSWPQEVGLDRAFRLRLHFSERRLLMIVLD
                     LIAINSALILALGLRPEHTVELALVGEQPFWCLILSCVWVGVATAFDVYESRVSGKLE
                     TSV"
BASE COUNT          507 a          940 c         1016 g          523 t
ORIGIN      
        1 ggagaagtcc cctgcggcga tttgccggaa ggtcgcgctc gcgagggacg gcggagagat
       61 cgaggtgtgg ggggacggca agcaaacgcg ctcgtactgc tatgtcgacg actgcgtcga
      121 gggaatccac cggttgatgc agaccgagga gcacgatccg atcaacctgg gaacggatcg
      181 gatggtgacc atcgatgagc tggtcgacat catcgccggg atcgccggca agcgcgtgat
      241 caagcgatac gacctgacca agccgcaggg cgtgcgcggg cgcaacagcg acaacacccg
      301 gctccggcgg gtgctgcgat gggagccccg ggtgccgctg gaagagggac tggcgaagac
      361 ctaccggtgg atccgggagc aactcatcaa agccgggaga gttccggaag aggctctgcg
      421 ataacgcgtt gcaccggcgc cgggatgggg cggtaggagt gcacgaggga ggggagatgt
      481 tcgcgggcgt ccccgtggcg gaggtggccg agtactggga caggcgcccg tgcaacatcc
      541 ggcactctcc cagggagatc gggacccgag cctactttga cgaagtcgag gcgcgcaagt
      601 ccttcgtcga gccgcatgcc ctccggttcg cggagttccc caggtgggcc ggcaagcggg
      661 tgcttgagat cgggtgcggc attggcacgc acacgatcgg atttgcccgg gggggcgcag
      721 tggtcacggc catggatctc tccgagcggt cactggagat cgcccggcgg cgcgccgcgg
      781 tctacgggct ggacggccgg atcacgttct acgcggggaa cgccgaggaa ctggacgcgg
      841 tcgtgcccgt cgagccctat gatctcatct tttcgtgggg ggtcatccat cacacccccc
      901 atccggggcg cgtcatcgat cagatccgcc ggtacacccg tccagggacc acggtcaaac
      961 tcatggtcta ccaccgccgg tcgtggaaga tcctcggcat cgtgctccgc cacgggcggg
     1021 ggcgtttctg gaagctggat gagctcgtcg cgcgccattc cgaggcggag acggggtgtc
     1081 cggtcaccta cacctaccgc ccgagcgacg tcgaggccct gctcgccggg tttgccatcc
     1141 gtgagatgtg ggtggatcat atctttccct accgcgtgaa cgagtatttg cagtaccgct
     1201 atgtcaaagc ctggtacttc cacgcgatgc ccggtccgat gtttcggtgg cttgagcgtc
     1261 acttcggctg gcacctgtgc gtcaccgcgg aggcccgctg aagcagcagg tatggcggca
     1321 ggcggaacga acagaaaggg ccaatggatg tgttgtcggt ggttggcctc ggaaaactcg
     1381 gcgcctgcac cgctgcctgc ctggctgccc gcggctttga cgtcctcggg atcgacatca
     1441 atcccggctt tgtccaggcg atcaatcgcg gcatcgcgcc cgtctccgaa ccccgcctcc
     1501 aggacctgat cacggccgcc gggccccaga tccgagcgac gcacgactat gcgcaggcga
     1561 tcgatgagtc agacgtcacc tttctggtcg ttccgacgcc cgtccagccg gacggagtgt
     1621 tttcggacgc ccacctgcgc gacgcgctga cgcgcttgtg ctccccgttc gagcggtccc
     1681 acaagcccta ccatctgttt gtggtcacct cgacggtctc tccggggacc acggaggggc
     1741 agctgatccc cctgatcgag tccctcaccg gcaagcggct gcacgagcac tttggcgtct
     1801 gctacaatcc ggagttcatc gcgctgggca gcgtgatcac ggatttcttg aaccccgacc
     1861 tcgtgctgat cggcgaaagc gatcgtgcgg ccggcgatct gctggaggga atctaccggc
     1921 aggtatgcga aaacgagccc gtcatggccc gcatggctct cgtcagcgcg gagatcacca
     1981 aggtcagcct gaattcctac gtcaccatga agatcagttt cgccaacacg ctcgcgagcc
     2041 tgtgcgaggc catccccggt gcggagatcg acctgattac ccgggcgttg ggcgcggaca
     2101 ggcgcgtgtc gccgcacacg ctcaagggcg gccctccgtt tgggggaccg tgcttcccga
     2161 gggataaccg ggccttcgcg gcgttcgcgc gaaggtacgg cgtcgacgcc aaattggcca
     2221 ccgcgtcgga cgagatcaac gcctctcagg ccgaacgcct cgcggccttg gtgctgcgcc
     2281 agctggccga ggtcaaggat cgcaccgtct cgatcctcgg gctcgcctac aaggccaaca
     2341 cgccggtggt cgaggagtca ccggcgatcc agatcatcca cgcgttgctg gaacggggta
     2401 tggacgtcat ggtctatgac ccgctggcca tggatgacac ccgggcggtg tttggggaac
     2461 ggattcgcta cgctccctcg gcgaaagcct gcgccgcgta cggggccgtc tgcgtcgtga
     2521 ccacccaatg ggaagagttt gcggggatcc aggccggcga ctttacccat aacccgacgg
     2581 tggtgatcga ttgctggcgc atgctggatc cggcgcggct cggcgccagg gtgaaatacg
     2641 tcgcgatggg cgcgtcgccc aggcggtaag ccatgagact cgaggactcc gggcgcatcc
     2701 gcagcgaggc gagaccctcc tggccgcagg aggtcggcct cgaccgtgcg tttcggctcc
     2761 ggctccactt ttccgaacgc cgtctcctga tgatcgtcct cgacctgata gcgatcaaca
     2821 gcgccctcat cctggccctg gggctgcggc ccgagcacac cgtggaactc gccttggtgg
     2881 gggagcagcc tttttggtgc ttgatcttga gctgcgtgtg ggtgggcgtg gccaccgcgt
     2941 tcgacgtcta tgagtcccgg gtgtcgggaa aactcgagac gagcgt
//
LOCUS       DASQGH010000251         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54906, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000251 DASQGH010000000
VERSION     DASQGH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54906"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1519)
                     /locus_tag="VEW91_04900"
     CDS             complement(<1..>1519)
                     /locus_tag="VEW91_04900"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HYM90955.1"
                     /translation="AQLGELDAQGRARARALADAFTGGGPQTDLFDPPDPAAQAVPVR
                     LNRVRLERGRTFGDVWLGWLLWRALQFDTVLERLLPPGREAIPWAAMAAILVLARLCE
                     PASELHLAETWYRQTALEDLFGVPAALVNDDRCYRALDQLLPHKTALEQHLVTRLGEL
                     FTLDYDLLLYDVTSTYVEGQALDNPLAQRGYSRDHRPDCKQICLALVVTREGLPLGYE
                     VFPGNRTDSTTVEEIIDAMEARFGLAQRIWVMDRGMVSAETLTWLQATGRRYLVGTPK
                     GELRKWARVLTDARDWAAVREGVEAKTCPGPDGTETFVLVRSVERREKEQAIHARFSA
                     RIEASLTRIAHRLARARRPVDRGHLERQLGRLLERNARAAGRYLIEVTPDPTVPAGLR
                     LAWTVRPEWDDWARWSEGCYVLRTNITDWPPEALWQTYIHLTDAEAAFRIQKSELGLR
                     PIWHQRPDRVQAHILVCFLAYVLWKTLEQWQRRAGLGQSPRTVLAELHRIQSADVT"
BASE COUNT          214 a          509 c          573 g          223 t
ORIGIN      
        1 gggtcacatc ggcgctttgg atgcgatgca gctcggcgag gactgtgcgg gggctctgcc
       61 ccaaccccgc ccgccgctgc cactgctcga gggtcttcca gaggacgtag gccaggaagc
      121 agaccaggat gtgcgcctgc acccgatccg ggcgctggtg ccagatgggg cggagaccca
      181 actcgctctt ctggatgcgg aaggccgcct ccgcgtcggt gaggtggatg tacgtttgcc
      241 aaagggcctc gggcggccag tccgtgatgt tggtgcggag gacgtaacag ccctcgctcc
      301 accgggccca gtcgtcccac tcggggcgga cggtccaggc gagccggagg ccggcgggga
      361 cggtggggtc aggggttacc tcgatgaggt agcgaccggc ggcccgcgcg ttccgctcga
      421 gcaggcggcc gagctgccgc tccaggtgcc cgcggtccac gggacgccgg gcccgggcga
      481 ggcggtgggc gatccgggtc aggctggcct cgatccgcgc gctgaagcgc gcgtggatgg
      541 cctgctcctt ctcccggcgc tcgacggagc ggacgaggac gaaggtctcg gtcccgtcgg
      601 ggcccggaca ggtcttcgcc tcgacgccct cccgcacggc ggcccagtcg cgcgcgtccg
      661 tgaggactcg ggcccacttc cggagctccc ccttcggggt gcccaccaga tagcggcggc
      721 cggtggcctg gagccaggtg agcgtctctg cgctcaccat gccgcggtcc atgacccaga
      781 tccgctgggc cagcccgaac cgcgcctcca tggcatcgat gatctcctcg accgtggtgc
      841 tatccgtccg gttgcccggg aacacctcgt accccagggg gaggccctcg cgggtgacga
      901 cgagcgcgag gcagatctgc ttgcagtcgg gccggtgatc gcggctgtag cctcgctggg
      961 ccagcggatt gtccagcgct tgcccctcga cataggtgct cgtcacgtcg tagaggagga
     1021 gatcgtagtc cagggtgaag agctcgccga gccgcgtgac gagatgctgc tccagggccg
     1081 tcttgtgggg aaggagttgg tccagggcgc ggtagcagcg gtcgtcgttg acgagggcgg
     1141 cgggcacgcc gaagagatcc tccagggcgg tttggcgata ccacgtctcg gccaggtgga
     1201 gctcgctcgc gggctcgcag aggcgtgcga ggaccaggat cgccgccatc gctgcccagg
     1261 ggatcgcttc ccggcccggg ggcaggagcc gctccagcac ggtgtcgaac tgcaaggcgc
     1321 gccagagcag ccagccgagc cagacatcgc cgaaggtgcg gccgcgttcc agccgcacgc
     1381 gattcagccg gacgggcact gcctgagcgg cgggatcagg gggatcgaag aggtcggtct
     1441 gcgggccgcc gccggtgaac gcatcggcca gcgccctggc ccgggcgcgc ccctgcgcat
     1501 cgagttcccc cagttgggc
//
LOCUS       DASQGH010000252         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_20883, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000252 DASQGH010000000
VERSION     DASQGH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20883"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VEW91_04905"
     CDS             complement(<1..1299)
                     /locus_tag="VEW91_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM90956.1"
                     /translation="MAPAGIDKTGRITEPVREIEVAHATDVLVVGGGIAGVMAALASG
                     RSNGRTLLVERFGSLGGTGTAAMMNLFYVPYAASRGLVRELFDRLIARGGAIPGEFVV
                     YDPELYKVTALEMLMEAGVEILLHTLVSDVIMEEKTLRGIVVENKSGRQAVLSRVTVD
                     ASGDADVAVRAGAPYIKGREEDGKMRPMTLIFRMGGVDVPRLVEYVKTHPEDFSPDPL
                     QCMLDLEHHMIRVFGFFKLVEEAKARGELWPECYYFRLESVLPERGVLTVNATRVYGV
                     DGTKAEDLSRAEIETRHQMIQLAAWARKWIPGFADSFILDSASTIGVRETRRIRGEYV
                     MTEDDILAGCHYDDVVGVDANQQNPRQQGGHPPDGEEGGPQDAEARELIARMFVYEIP
                     YRCLVPRGVDGLLVAGRCLSVDHHADVYTRNQGTAMASGVA"
     gene            complement(1336..>1778)
                     /locus_tag="VEW91_04910"
     CDS             complement(1336..>1778)
                     /locus_tag="VEW91_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM90957.1"
                     /translation="WLSTPGTALIGLVAADVWEWTPLMGLLLLSALQAVSREMVEAAR
                     VDGASGLQVLRYVTLPQIAGMMAVSAGLRFMDAFRELDKVLIMTGGGPGTSTDFLSMH
                     VYKAAFRFFTLGYAATVVVVTLLFLAVVYGFYLRAVRNLAISYS"
BASE COUNT          322 a          632 c          528 g          296 t
ORIGIN      
        1 ggccacgccc gaggccatcg cggtgccctg gttacgagtg tacacgtccg cgtggtgatc
       61 cacggacagg cagcggccgg cgacgagcag gccgtcgact ccgcggggca caagacaccg
      121 gtacgggatc tcgtagacga acatgcgggc gatcagctcg cgcgcctccg cgtcttgcgg
      181 tccaccctcc tcgccgtcag gcgggtggcc gccctgctga cgtggattct gctggttggc
      241 gtccacgccc acgacgtcgt cgtagtggca cccggcaagg atgtcatcct cggtcatgac
      301 gtactcaccc ctgatgcggc gggtctctcg gacaccaatg gtactggcgg agtcgagaat
      361 gaacgagtcc gcgaatcccg gaatccactt gcgagcccac gcagcgagct gaatcatctg
      421 gtggcgcgtc tcgatctcgg cccgcgacag atcctctgcc ttggtgccgt ccacgccgta
      481 gacgcgggtc gcgttcaccg tcagtacgcc gcgctccgga agcacggact ccaagcggaa
      541 gtagtagcac tccggccaca gctcgccccg agccttggcc tcctcgacca gcttgaagaa
      601 gccgaagaca cggatcatgt ggtgctccag atccagcatg cactggagtg gatcgggcga
      661 gaagtcctcg gggtgagtct tgacgtactc gaccaaccga ggcacgtcca cgccccccat
      721 gcggaagatt aacgtcatcg gccgcatctt gccgtcttcc tcgcggcctt tgatgtacgg
      781 ggcgcccgcg cggacggcga cgtcagcgtc gccgctggcg tcgaccgtca cacggctgag
      841 aacggcctgg cggcccgatt tgttctcgac cacgatgccc cgaagcgtct tctcctccat
      901 gattacatcg ctgaccaggg tgtggagcag gatctccact ccggcctcca tcaacatctc
      961 aagcgcggtg actttgtaca gctcagggtc gtagacgacg aactcccccg ggattgcccc
     1021 gcccctggcg ataaggcggt cgaatagctc acgcaccagc ccccgcgacg cggcgtaagg
     1081 cacgtagaag agattcatca tcgccgcggt tcccgtgccg ccgagcgatc cgaagcgctc
     1141 gacgaggagg gtccgcccgt tgctgcggcc ggatgcaagc gctgccatca ccccggcgat
     1201 cccgccaccc accacgagga cgtccgtcgc atgcgccact tcgatctccc ggacgggctc
     1261 cgtgatcctc cccgtcttgt caatcccggc cggcgccatc accttgcacc tccctacgtt
     1321 tctgagctgt ttccgtcaac tgtatgagat cgcgaggttg cggaccgccc gcaggtagaa
     1381 gccgtacacg acagcgagga acagcagggt taccaccacg accgtcgccg cgtaccccag
     1441 cgtgaagaac ctgaaggccg ccttgtagac atgcatgctg aggaagtccg tcgacgtccc
     1501 cgggccgcct cccgtcatga tgagtacctt atcgagctcc cggaacgcgt ccataaagcg
     1561 caaccctgcg ctcaccgcca tcattccagc gatctgcggg agggtcacat accgcagcac
     1621 ctggagtccc gacgccccgt ccactctcgc cgcctccacc atctctcgcg agacggcctg
     1681 cagtgccgag agcagcagaa gccccatcaa cggcgtccac tcccatacgt cggcggcaac
     1741 gaggccgatc aacgcggtcc cgggcgtcga gagccacg
//
LOCUS       DASQGH010000253         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32378, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000253 DASQGH010000000
VERSION     DASQGH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32378"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VEW91_04915"
     CDS             complement(<1..548)
                     /locus_tag="VEW91_04915"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM90958.1"
                     /translation="MRAFLARRLGLGAVTLVAMSMVIFVLLRVAPGNIVDLVFQSAGF
                     VDPAEKQRLTHELLLDRPIAIQYLEWAKAFLHGDLGKSYRYGLPAWQIIRPRIPITIE
                     LAVLSLLVSIVVGVPTGVVSATRQNTWADYGLRVFSLTGLSLPSFWLGMVIILLLVRA
                     FGWIPSMLYVAPWVDLRAHILQ"
     gene            complement(567..1988)
                     /locus_tag="VEW91_04920"
     CDS             complement(567..1988)
                     /locus_tag="VEW91_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYM90959.1"
                     /translation="MSQDLCALPATALVEAIRTKRVSALEVTDAVLARIDRVNPVVNA
                     FVTVVADQAREAARAADAAVMRRPPSELGLLHGVPVSIKDLVLTKGIRTTFGSRAFAD
                     HVPTEDALIVQRLRAAGAIILGKTNTPELGAGGNTKNALFGPTRNPWRLSHTCGGSSG
                     GAAVALATGMGPLAQGSDTGGSLRVPASFCGVIGFRTSPGVVPVYPTPLAWDTLSVAG
                     PMARTVGDTALMLAAIAGPDDRAPISIPVNPAEWLEAVRTPDVRGWRVAWSADLGVTP
                     VEPEVARVAEEAARALLDLGGALEEAHPDFAGVREIIGAPRAARMATVHAGLLPRWRD
                     QMFPPLVRNIEQGLTLTATQWGRAEMERTALWHRVRAFFERYDLLVTPTVAVPPFPLE
                     WDYPPEINGHPVESYTDWFLLTYAITLTGLPAISVPAGWTADGLPVGLQIVGRRRGEA
                     AVLRAAAALEAARPWAGRRPPVT"
     gene            complement(2056..2595)
                     /locus_tag="VEW91_04925"
     CDS             complement(2056..2595)
                     /locus_tag="VEW91_04925"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM90960.1"
                     /translation="MVEYRLMTTPSMNFAHFFDYLLTARGRLLEWVRAQPSAVYTRTF
                     PIGLGSIRATLIHTAAAEWAYVERLSGREVSLPDSPFTAERQPEFDPFAAAWERRAPI
                     TRQALAGLGDPARPVEFVSRMGPKPMRVRTIAGGIAGQLLFHEVHHRAQVMAMLRQVG
                     VAAENLDYSVLVFERTPVD"
     gene            2700..3335
                     /locus_tag="VEW91_04930"
     CDS             2700..3335
                     /locus_tag="VEW91_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYM90961.1"
                     /translation="MVFDLDGTLIDSYALIAAAFRHAARTVLGRFLTDEEVLAHWGEP
                     LPVRAAHLASDRAAEVVSAYTAYYDAHHDRLCRPFPGVIEMLAQASARGCALGIATSK
                     RRRSTMQAMDAFGFAAWIRVAVTAEDVRAPKPSPDPVTLVLERLGVRPSEALLVGDGV
                     FDIRAARAAGVRSVAAMWGTREAAAVLAAGPDYVAMRPGDVVPLLGSGPSG"
     gene            complement(3325..4107)
                     /locus_tag="VEW91_04935"
     CDS             complement(3325..4107)
                     /locus_tag="VEW91_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYM90962.1"
                     /translation="MVKLGIVGDIHCGPDAGAQLGSRAPAMLEVFSEAMTAFRPDCII
                     DLGDRINDVASGQDRQRTAWVRRQLLEIGVPVFHLLGNHDIVNLTKAEVSEVLGKRGS
                     FESVEVGGVRLVLLDSQDPPFERIGGEIGPDQRAWLEGVLEDSHRPALVFCHHPLDEQ
                     GVDGHWYFAAHPGHACARARAPVRRILERSGEVCAVFSGHMHWTRTTAINGIPYVTLG
                     SLVDGGFTHGRPCGTFAQVTVQGKTTDVQVAGLLPDQFRLTR"
     gene            complement(4101..>5350)
                     /locus_tag="VEW91_04940"
     CDS             complement(4101..>5350)
                     /locus_tag="VEW91_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYM90963.1"
                     /translation="NPRGDTMPKTQERYELELHCTRCRRTTTHSVATGDRTLSRVMCI
                     ACGRAVAVDTLQFMEQYVDSVMRRLMAKPFEITTEFTRSPREFITSLPSRMLTKPFRV
                     AAELRATMDIVRLRRRTRRPMAPTLPALPGELAPVERRCRVLLSAPLMWAHTPDDLLV
                     AARELGYDGVEMWAYQLSWDRADAAALGTRAKAMGLALTLHALSWDLNLSSRLDTIRA
                     ASLDAVHRSVEQARELGASLVIVHPGHITVPFDDAEAYWPYLVASIRELADHAAASGV
                     RVGVEHMEPRQGEYVITADQANRLVRDVGRSNVGTVLDVAHIPWGEDESAFAAGLEHI
                     IHVHFSDADESRLHLPLGQGGRNLVRFLGGLRAYTGAIALEGFSIEAGTDLARWNKAR
                     FEELWREATAGIGEVTRGGPQPW"
BASE COUNT          790 a         1898 c         1910 g          752 t
ORIGIN      
        1 aactgcagga tgtgggcccg tagatccacc cacggagcga cgtagagcat cgaggggatc
       61 catccaaatg cccggacgag cagcagaatg atgaccatgc ccagccagaa cgacggcagc
      121 gacagcccgg tcaggctgaa gaccctgagg ccgtagtccg cccacgtgtt ctgccgggtc
      181 gcgctcacga cgccggtcgg cacccccacc acgatcgaga ccaggaggga gaggaccgcc
      241 agctcgattg tgatcgggat gcgggggcgg atgatctgcc aggccgggag cccgtagcgg
      301 tacgactttc cgagatcccc gtggagaaag gccttggccc attcgagata ctggatggcg
      361 atgggccggt ccagcaggag ttcgtgggtg aggcgctgct tctccgccgg atcaacgaac
      421 ccggcggact ggaagacgag gtcgacgatg ttccccgggg cgacgcggag gaggacgaag
      481 atgaccatcg acatcgccac aagcgtcacg gcccccagcc ccaaccgccg cgcgaggaac
      541 gcccgcatgg gaggtcctac cgcgcctcag gtcaccggcg gccggcggcc ggcccagggc
      601 cgcgccgcct cgagcgcggc ggccgcccgc agaacggcgg cctcgccacg gcggcgtccg
      661 acgatctgca gccccaccgg cagcccgtcg gcggtccagc ccgccgggac cgagatcgcc
      721 gggagccccg tcagggtgat ggcgtaggtg agcaggaacc agtcggtgta gctctcgacg
      781 gggtgcccgt tgatctccgg agggtagtcc cactcgagcg gaaacggggg aacggcgacg
      841 gtcggggtga cgagcaggtc gtaccgttca aagaacgccc ggacccggtg ccagagcgcg
      901 gtgcgttcca tctccgctct gccccactgg gtggcggtca gggtcaggcc ctgctcgatg
      961 ttccggacga gcgggggaaa catctgatcg cgccaccggg gcagcaatcc ggcgtggacc
     1021 gtggccatgc gcgccgcgcg gggcgcaccg atgatctcgc gcactccggc gaagtccggg
     1081 tgcgcctcct cgagcgcgcc ccccagatcg agcaatgccc gcgccgcctc ttccgccacg
     1141 cgagccacct cgggctcgac cggggtcacc ccgaggtccg cgctccaggc cacacgccac
     1201 ccgcgcacgt cgggggtgcg gaccgcctcc agccactcgg cggggttgac cggaatggag
     1261 atcggggcgc ggtcatccgg gccggcgatg gccgccagca tcagcgccgt gtcccccacg
     1321 gtgcgcgcca tgggaccggc gaccgagagc gtgtcccacg ccagcggggt cgggtacacc
     1381 ggcaccaccc ccggcgaggt gcgaaacccg atcaccccgc agaagctcgc cgggacccgc
     1441 agcgaccctc ccgtatcgct tccctgggcg agcggcccca tcccggtggc cagggcgacc
     1501 gccgcccctc cgctggatcc tccgcacgtg tggctcaacc gccagggatt gcgggtcggt
     1561 ccgaagaggg cgttcttcgt gttgccgccc gcgcccagct ccggggtgtt ggtcttgccc
     1621 aggatgatcg ccccggccgc ccggaggcgc tggacgatca gggcgtcctc ggtcgggacg
     1681 tgatcggcga acgcgcgcga gccgaacgtc gtgcggatcc ctttggtgag cacgaggtcc
     1741 ttgatcgaga cggggacgcc gtggaggagg ccgagctcgg agggcgggcg ccgcatgacg
     1801 gcggcgtccg cagcgcgcgc ggcctcccgg gcctggtcgg ccaccaccgt gacgaacgca
     1861 ttcacgaccg ggttgacccg gtcgatccgc gccagcaccg catcggtgac ttccagcgcc
     1921 gagacccgct tggtgcggat cgcctccacg agcgcggtcg cgggcagcgc gcagaggtcc
     1981 tggctcatcg gccgcctcgg cgggctggcc tggcgtgccg cgtggacagc gcccgcctcc
     2041 gggtgggtcc gctgatcagt cgacgggggt gcgttcgaac accagcaccg agtagtcgag
     2101 gttctcggcc gcgaccccga cctgccgcag catcgccatc acctgggcgc ggtggtgcac
     2161 ctcgtgaaac aggagctgcc cggcgatgcc ccccgcgatg gtgcggaccc gcatcggctt
     2221 cgggcccatg cgcgacacaa actcgaccgg ccgggcgggg tccccgaggc cggccagcgc
     2281 ctgccgggtg atgggagcgc ggcgctccca cgccgccgca aatgggtcga actcaggctg
     2341 acgctccgcg gtgaacggcg agtccggaag cgagacctcg cggccgctca ggcgctccac
     2401 gtacgcccac tccgcggccg cggtgtggat cagcgtggcg cggatcgatc cgaggccgat
     2461 cggaaacgtc cgcgtgtata ccgcggacgg ctgggcgcgg acccattcga gcaggcggcc
     2521 ccgggcagta agcaggtaat cgaagaaatg cgcgaagttc atcgacgggg ttgtcatgag
     2581 gcggtattcc accacccgtc gggctccccc tgcccccggc gcctccgccg ggaggctgag
     2641 aggagttggg gagcgggcgg cggaactgcc cccgtctagg gccaatgcgc gtcgccgcgg
     2701 tggtattcga tctggacggc accctcatcg actcgtacgc gctgatcgcg gccgccttcc
     2761 gtcacgcggc ccgcacggtg ttgggccgct tcctcaccga cgaggaggtg ctggcccact
     2821 ggggggagcc gcttccggtg cgggccgccc acctggcctc cgaccgggcg gcggaggtgg
     2881 tgagcgccta caccgcctac tacgatgcgc accacgaccg gctgtgtagg cccttcccgg
     2941 gagtcatcga gatgctggcc caggcgtctg cgcgcgggtg cgcgctcggc atcgctacgt
     3001 cgaagcgccg gcggtcgacg atgcaggcga tggacgcgtt tggctttgcg gcgtggatcc
     3061 gggtggcggt caccgcggaa gatgtccgcg cacccaagcc ttcccccgac ccggtgacgc
     3121 tggtcctcga gcgcctgggg gtgcgcccct cggaggcgct gctcgtcgga gacggcgtct
     3181 tcgacatccg ggcggctcgg gcggccgggg tacgcagcgt tgccgcgatg tgggggaccc
     3241 gcgaggccgc ggcggtgctc gcggccggcc ccgactacgt cgcgatgcgg cccggcgacg
     3301 tcgtcccgtt gctgggatcc ggccctagcg ggtgaggcga aactggtccg gcaggagccc
     3361 ggcgacctgt acgtcggtgg tcttcccctg caccgtgacc tgggcaaacg tcccgcacgg
     3421 ccggccgtgc gtgaaccccc cgtccacaag cgacccgagc gtcacgtagg ggatgccgtt
     3481 gatcgcggtg gtgcgggtcc agtgcatgtg gccggagaac acggcgcaca cctccccgga
     3541 ccgctcgagg atcctccgga ccggcgcccg cgcccgtgcg caggcgtgcc ccgggtgggc
     3601 ggcaaaatac caatgcccgt ccacgccctg ctcgtcgagc gggtggtggc agaagacgag
     3661 cgccggccgg tgggaatctt ccaggacccc ctcgagccac gcccgctggt cgggcccgat
     3721 ctctccgccg atccgctcga acggtgggtc ctggctgtcg agcagcacca gccgcacccc
     3781 gcccacctcc acggactcga acgatccccg cttgcccagc acctccgaca cctcggcttt
     3841 ggtcaggttg acgatatcgt ggttgcccag cagatggaac accggcacgc cgatctccaa
     3901 cagctgccgc cggacccacg ccgtgcgctg acggtcctgg ccactggcga cgtcgttgat
     3961 gcgatccccg agatcgatga tgcagtcggg ccggaacgcg gtcatcgcct cgctgaagac
     4021 ctccagcatg gcgggcgcgc ggctgccgag ctgggccccg gcatcgggcc cgcagtggat
     4081 gtccccaacg atgccgagct tcaccacggc tgcggtcctc cgcgcgtgac ctcgccgatc
     4141 cctgccgtgg cctcacgcca gagctcctcg aagcgggcct tgttccaccg cgccaggtcg
     4201 gtcccggctt cgatcgagaa tccctcgagc gcgatcgctc cagtgtaggc gcggaggccc
     4261 cccagaaacc ggaccaggtt ccgcccgccc tggccgaggg gaagatggag ccgcgattcg
     4321 tcggcgtcgc tgaagtggac gtggatgatg tgctccaggc ccgcggcaaa cgcggattcg
     4381 tcctcgcccc acgggatgtg cgccacgtcg agcacggtcc ccacgttcga tcggcccacg
     4441 tcccgcacca ggcggttcgc ctgatcggcc gtgatgacgt attccccctg gcgtggctcc
     4501 atgtgctcga cccccacgcg caccccggag gcggcggcat ggtccgcgag ttcccggatg
     4561 ctcgccacga gatagggcca gtacgcttcc gcatcgtcga acgggacggt gatgtggccc
     4621 ggatgcacga tgaccaagga tgcgccgagc tcccgggcct gctccaccga tcggtggacg
     4681 gcgtccaggg aggccgcccg aatcgtgtcc aggcgtgagg agaggttgag atcccagctg
     4741 agggcgtgca gggtgagcgc cagccccatc gccttggccc gtgtcccgag ggccgcggca
     4801 tcggccctgt cccaggacag ctggtacgcc cacatctcga ccccgtcgta cccgagctcc
     4861 cgggcggcca cgaggaggtc gtcgggggtg tgggcccaca tcagcggggc gctgagcagc
     4921 acccggcagc gccgctcgac gggggcgagc tccccgggaa gcgccgggag ggtgggcgcc
     4981 atcggccggc gcgtccgtcg ccgcaggcgg acgatgtcca tggtcgcccg cagctccgcc
     5041 gccacccgga acggtttggt gagcatccgg ctcggcagcg aggtgatgaa ctcgcggggg
     5101 ctgcgcgtga actccgtcgt gatctcaaac ggcttggcca tgagccggcg catcacgctg
     5161 tcgacgtact gctccatgaa ctggagggtg tccacggcca ccgctcggcc gcaggcgatg
     5221 cacatgacgc gcgacagcgt gcgatcgccg gtcgcgaccg agtgggtcgt ggtccgtcgg
     5281 cagcgggtgc agtggagttc gagttcgtac cgttcctgcg ttttcggcat ggtgtctccc
     5341 cgcggattct
//
LOCUS       DASQGH010000254         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18169, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000254 DASQGH010000000
VERSION     DASQGH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18169"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VEW91_04945"
     CDS             complement(<1..1089)
                     /locus_tag="VEW91_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM90964.1"
                     /translation="MLRRARDLHYGWAIVAALSVTETISWGIIYYSFPVFLQAMERDL
                     GASRVAITGALSVGLGVSALAAVPVGWWLDRFGPRALMTAGSCLAAILLIAWSRVESL
                     GALYLVWCAMGLAMAATLYEPAFAAVVQWFARHRDRALLIVTLAAGLASTIFMPVAAW
                     LLGRQGWRTATLTLAITLALTTIPIHAVVLRRPPRAGREPDRGPATPAIGGRSLHAAL
                     RSVVFWVLAGVFAVGTFTTTSISVHLIPYLTQHGYSAQFAAVAVGWMGAMQLLGRLLF
                     LPVASRLGPVGVTASVCLTQGAAMALLAIAVQLGGLAPAIVLLGAANGMATLARATTV
                     VETFGRRYYGSIGGAIAVAANSARALGPV"
     gene            complement(1089..2180)
                     /locus_tag="VEW91_04950"
     CDS             complement(1089..2180)
                     /locus_tag="VEW91_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYM90965.1"
                     /translation="MRRTRVVLLGVDGCPAHLVSPASTPRLWMLGETGGLTLEGRAPL
                     PSTTYPCFATLLTGCLPRRHAVRTTAWDPGAARGWAGRRRVAVPTLFDACRGAGFGSA
                     AVQGDHLLHAVLGTEAAGMTWPPGGTIPPDTPCDAHGYPTNDAVTPHLLAAAADPATD
                     FLFGQLNEGDTLGHDLGPDDPATRACYAAADRIIGELLDLLQARWEEIVVVVVSDHGM
                     EPRSDRPPIDLMASPTVRAVAADVLGDGGAALVRVRDGVEEAEAGAALAEVPGIAGWS
                     RDAEGLLIAEARPGWIFRAPRLPARGYHGGRMTARTFAVVGGGHPAVPVIARSFPARP
                     PHLADWAPTIARLLRIELPGVDGQDLSTG"
     gene            complement(2248..>3044)
                     /locus_tag="VEW91_04955"
     CDS             complement(2248..>3044)
                     /locus_tag="VEW91_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM90966.1"
                     /translation="SCGIHCAEHIGEVIEFENPETVAAVIVETITGSNGRIVPPPEYY
                     PRLREICDRYGVLLICDEVMAGFGRTGKWFAIEHYNVVPDLMTCGKGITSGYVPLGAV
                     VASDRVAAFFESHVLLTGATYSGHPLACGAGVATLEAYHEEGLIDRCRILGDRLMSGL
                     RAIGERHPAVGEVRGRGLFACIELVRDRETRAPLIPWTPRGTRETHPAVTGTLRAMKE
                     RGVYAYTKWNMIMLAPPFVITEAELEEGLQVIDAALSVADAHVSAA"
BASE COUNT          483 a         1115 c         1074 g          372 t
ORIGIN      
        1 gacggggccc agggcgcggg cgctgttggc cgcgaccgcg atggccccgc cgatgctgcc
       61 gtagtaacgc cgcccgaagg tctcgacgac ggtggtcgcc cgggccagcg tcgccatccc
      121 gttggcggcg cccaagagga cgatcgccgg ggccaacccc cccagttgga ccgcgatcgc
      181 caggagcgcc atcgccgcgc cctgggtgag gcagaccgac gccgtcacgc cgaccggacc
      241 gagccgcgac gcgaccggga gaaagaggag gcgtccgagg agttgcatgg cgcccatcca
      301 cccgaccgcg accgccgcga attgcgctga gtacccatgc tgcgtgaggt acggaatgag
      361 atgcacggag atcgacgtcg tggtgaacgt gccgacggcg aagacgccgg ccaggaccca
      421 gaacaccacg ctcctcagcg cggcgtgcag cgaacgcccg ccgatcgccg gcgttgcggg
      481 tccgcggtcc ggttcgcggc cggcccgggg agggcggcgg agcaccactg cgtggatcgg
      541 aatcgtcgtg agggccaggg tgatggccag cgtcagggtc gcggtacgcc agccctgccg
      601 cccaagcagc cacgccgcaa ccggcatgaa gatggtgctg gccagtccgg cggcgagggt
      661 gacgatcagg agcgcccgat cgcggtggcg ggcaaaccac tggaccactg cggcgaaggc
      721 gggctcgtac agggtcgcgg ccatcgccag ccccatcgcg caccagacga ggtagagggc
      781 cccgaggctc tccacccgcg accaggcgat gaggagaatc gcggcgaggc acgagccggc
      841 cgtcatgagg gcccgcggcc cgaaccggtc gagccaccac ccaaccggca cggccgccag
      901 ggccgagaca cccaggccca ccgagagggc cccggtgatc gccacccgcg aggccccgag
      961 atcgcgctcc atggcctgga ggaagaccgg aaagctgtag tagatgatcc cccacgagat
     1021 ggtctcggtg acggacagcg ccgcgacgat ggcccagccg taatgaaggt cccgtgcgcg
     1081 ccgcagcact agcccgtgga aaggtcctgc ccgtccactc cgggcagctc gatcctgagc
     1141 aaccgggcga tcgtcggcgc ccagtcggcc aggtgcggag gccgggccgg gaacgaccgc
     1201 gcgataacgg ggaccgcggg gtgtccaccc ccgaccaccg caaatgtgcg ggcggtcatc
     1261 ctcccgccgt ggtaccccct cgccggaagc cgcggagcgc gaaagatcca tccggggcgc
     1321 gcctctgcga tgaggagccc ctcggcatcc cggctccacc cggcgatccc cgggacctcg
     1381 gcgagggcgg cgccggcttc ggcctcctcg accccgtccc gcacgcggac cagcgccgcc
     1441 ccgccgtcgc cgagcacatc cgcggccacc gcacgcaccg tgggcgacgc catgagatcg
     1501 atcgggggcc gatcgctgcg gggctccatc ccgtggtccg agacgacgac cacgacgatc
     1561 tcttcccagc gcgcctggag gaggtcgagc agctcaccga tgatccggtc ggcggcggca
     1621 taacacgcgc gcgtcgccgg atcgtccggg ccgaggtcgt gaccgagggt gtcgccctcg
     1681 ttgagctggc cgaagagaaa gtccgttgcg ggatcggcag cggcggcgag caggtggggc
     1741 gtgaccgcgt cgttggtggg gtagccgtgc gcgtcgcacg gggtgtctgg agggatcgtc
     1801 cccccgggcg gccaggtcat cccggcggcc tcggtcccca gcaccgcgtg cagcaggtga
     1861 tccccctgga cggccgcgct cccgaacccc gcgccccggc acgcatcgaa gagggtcggc
     1921 accgcgaccc gccgccggcc tgcccaccct cgggccgctc ccggatccca ggcggtcgtc
     1981 cgcaccgcat gccgccgcgg aagacacccg gtcaggagcg tggcgaagca cggataggtc
     2041 gtggacggga gcggggcgcg cccctctagg gtgaggcccc ctgtctcccc gagcatccag
     2101 aggcgcggcg tcgatgcggg ggacaccagg tgcgcggggc agccgtcgac gccgagcagc
     2161 acgacgcgcg tcctgcgcac gactcactcg tgagtgggca actcgttgac ggtggccgga
     2221 tcacccttcg ccgtcgccgc ccccgcatca cgcggcggac acatgcgcgt ccgccaccga
     2281 cagcgcggcg tcgatgacct ggaggccttc ctccagctcc gcctcggtga tgacgaaggg
     2341 cggggccagc atgatcatgt tccacttggt gtaggcgtag acaccccgct ccttcatggc
     2401 gcgcagcgtg ccggtcaccg cggggtgcgt ctcgcgcgtg cccctcgggg tccacggaat
     2461 caatggggcg cgcgtctccc gatcgcgcac cagctcgatg caggcaaaca gcccgcggcc
     2521 ccggacctcc cccacggcgg ggtggcgctc gccgatcgcg cggagcccgg acatcagccg
     2581 gtcaccgagg atccggcagc ggtcgatcaa cccctcctca tggtacgctt cgagcgtcgc
     2641 cacgccggcg ccgcacgcca gcgggtggcc gctgtacgtc gcgccggtca acagcacgtg
     2701 ggactcgaag aacgccgcca cccggtccga cgcgaccacc gcgccgaggg gcacgtaccc
     2761 cgaggtgatc cctttcccgc aggtcatgag atcggggacc acgttgtagt gttcgatggc
     2821 gaaccatttc ccggttctgc cgaaccccgc catgacctcg tcgcagatca ggagcacccc
     2881 gtatcggtcg cagatctcgc gcaggcgcgg gtagtactcg ggcggaggga cgatccgccc
     2941 gttgctcccg gtgatcgtct ccacgatgac cgccgcgacc gtctccggat tctcgaactc
     3001 gatgacctct ccgatatgct cggcgcaatg gatgccgcac gacg
//
LOCUS       DASQGH010000255         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_3999, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000255 DASQGH010000000
VERSION     DASQGH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3999"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VEW91_04960"
     CDS             complement(<1..264)
                     /locus_tag="VEW91_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM90967.1"
                     /translation="MTTGTGRLDYFGIDPLLTDEERLTRETVARLVDREVIPHIGRAW
                     LAGEFPRELVPKLGALRLFGANLPQEHGCAGMSNIAYGLIMQEL"
     gene            396..809
                     /locus_tag="VEW91_04965"
     CDS             396..809
                     /locus_tag="VEW91_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90968.1"
                     /translation="MPDTIRLVDYFYIETADRPGEGARVLATLRDARVYLQAFHAFPK
                     GRRAQLDFVPSDPAAFKAAARSAGWKVVGPKKALAIEGDDRVGALVGYFTRLAEAKIN
                     VTASDAIAAGAGRFGAIVWVKPRDVKRAAKTLGIG"
     gene            complement(846..>1964)
                     /locus_tag="VEW91_04970"
     CDS             complement(846..>1964)
                     /locus_tag="VEW91_04970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYM90969.1"
                     /translation="AMLADYGADVLKVEPPGEGDGARAWGPPFVGGESVYFLSVNRNK
                     RSLTLNLRHPEGVALLLRLVDRADVVVENFTPGTMARLGIGDEVLRRRNARLIYCAIS
                     GFGQSGPYRDRPAFDLILQGMGGVMGTTGEEGGAPVRVGIPIADIAGGMYAAYAIVLA
                     LLARGRTGVGQVLDVSLMDAQISWLSYYVGIYLASGEVPPRRGSAHATVVPYQAFDCA
                     DGRYITLGTGNDRLFRRFCKVADLPLADDPRFQTNPDRIRHRTELIPLLEAVFRARPA
                     AAWLEALGAAGVPCGPIATVDEIVRDPQVRAREMLVEIDHPSAGRIAVPGLPIKCSGT
                     PGSIRRPPPRLGEHTTEVLTELGVPADEIPRLRRLGAI"
BASE COUNT          320 a          669 c          693 g          282 t
ORIGIN      
        1 gagctcttgc atgatgagcc cgtaggcgat attgctcatt cccgcgcacc cgtgctcctg
       61 ggggaggttg gcgccgaaca gccgaagcgc gcccagtttg ggcaccagct cccgcgggaa
      121 ctcgccggcg agccacgccc ggccgatgtg gggaatcacc tcccggtcca ccagcctcgc
      181 gacggtctcg cgggtcaggc gctcctcgtc ggtaagcagc gggtcgatgc caaagtaatc
      241 gagcctgccg gtcccggtag tcatggtgca tccattcgca ggatgcacca gggtctcctg
      301 tgccgcgtcc tcacacgagg gggatcagag gaggaggaaa ccgcggtggc tgagtaactc
      361 tgtcgcaaga catgcagcct aaggggggga gaccgatgcc cgacacaatc cggctcgtcg
      421 actatttcta cattgagacc gccgatcggc ccggggaggg cgcccgagtc ctggcgaccc
      481 tgcgggatgc ccgcgtctat ctgcaggcct ttcacgcctt cccgaagggt cggcgggcgc
      541 aactcgactt cgtcccgtcg gatccggcgg ccttcaaggc ggcggccagg agcgcagggt
      601 ggaaggtcgt cgggccgaag aaggcgcttg cgatcgaggg tgacgaccgc gtcggggcgc
      661 tggtgggcta tttcacgagg ctggccgagg cgaagatcaa cgtgacggca agcgacgcga
      721 tcgcggccgg cgccggccga ttcggggcca tcgtgtgggt caagccgcgt gacgtgaaac
      781 gagcggcgaa gacgctgggc atcggttgag cgagaacccc gccggggggg cagcccgtgg
      841 ctccctcaga tggcccccaa acgcctcagc cggggaatct cgtcggccgg cacccccaac
      901 tccgtgagga cttccgtcgt gtgctccccc agccgggggg gcgggcggcg gatcgatccg
      961 ggcgttccgg agcatttgat cgggagccct ggcaccgcga tgcgcccggc ggatgggtgg
     1021 tcgatctcca ccagcatctc ccgcgcccga acctgcgggt cgcgaacgat ctcgtccacg
     1081 gtggcaatcg gtccgcacgg cacgcccgcg gcgccgagcg cctcgagcca cgcggccgcc
     1141 gggcgcgccc ggaacaccgc ctcgagcaga gggatcaact cggtgcggtg gcggatgcga
     1201 tcggggttgg tctggaagcg cggatcgtcg gccaggggga ggtcggcgac cttgcaaaac
     1261 cggcgaaaga gccggtcgtt gccggtcccg agcgtgatgt agcgcccgtc ggcacagtca
     1321 aacgcctggt acggaacgac ggtggcgtgc gcggagcccc ggcggggcgg gacctccccc
     1381 gaggcgagat agatcccgac atagtacgag agccaggaga tctgcgcatc catcagcgac
     1441 acgtcgagga cctggccgac gccggttcgg ccgcgggcga gcaaggcaag gacgatcgcg
     1501 taggccgcgt acatcccgcc ggcgatgtcg gcgatcggga tcccgacccg cacgggggcg
     1561 ccgccctcct caccggtcgt gcccatgacg ccgcccatcc cctggaggat caggtcaaac
     1621 gccggccggt ccctgtacgg gccgctctgt ccgaaccccg agatcgcgca gtagatcagc
     1681 cgcgcgtttc gccgtcgcag gacctcgtcg ccgatcccca gccgggccat cgtcccgggg
     1741 gtgaagttct cgaccaccac gtccgcccgg tcgacgagcc ggagaagaag cgccaccccc
     1801 tccggatgac ggagattgag cgtcaggctg cgcttgttcc ggttcacgct taggaagtag
     1861 acgctctccc ctcccacgaa gggcggtccc caggcgcgcg ccccgtcgcc ttcgcccggg
     1921 ggctcaacct tgaggacgtc ggccccgtaa tccgcgagca tcgc
//
LOCUS       DASQGH010000256         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4000, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000256 DASQGH010000000
VERSION     DASQGH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VEW91_04975"
     CDS             <1..1062
                     /locus_tag="VEW91_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYM90970.1"
                     /translation="PRQTELREYDVADPGPGEVLLQVLRANVCGSELHIWRGHHPTVK
                     MGSVLGHEMMGRVLALGQGVEADYASAPLRVGDRVVCPYFITCRRCSACQAGLFNLCA
                     NCYRYWSQPADAPPHFHGAFATHYYVHPDQYLYKVPDPVPDAAAASANCALSELLFGL
                     DQAGVAAGEQVVVQGAGGLGLCALAVAKARGARTIVIDGVPRRLELAHRFGADLVVDL
                     RDDDTPERRAARIQALTGGMGADVAVEVTGVPAAFAESIHLVRPGGRVVELGNISPGH
                     TTPFDPGTLTRRGVQILGTIRYHPWYLKRALDFLVATRGRFPFEALLDAEYPLRDVGR
                     ALEDSDQRKVTRASLLVGR"
     gene            complement(1115..>1553)
                     /locus_tag="VEW91_04980"
     CDS             complement(1115..>1553)
                     /locus_tag="VEW91_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HYM90971.1"
                     /translation="TWRQYEREWANHPYPVTLAANNIVALDRALDRIFAEGLEVRFER
                     HRRAAAQLREGLDRLGFKVFVDRRWASPTVTVAYPPEGVKASALMETLRIQHRIAVAG
                     GLEHLTGRVIRIGHLGNQATPETIRRLLGALEACLRPGVPSSP"
BASE COUNT          220 a          552 c          557 g          224 t
ORIGIN      
        1 cccaggcaga ccgagttgcg ggagtacgac gtggccgacc ccgggccggg cgaggtgctg
       61 ctccaggtgc tcagggccaa cgtctgcggt tcggagctcc acatctggcg cgggcaccac
      121 ccgacggtca agatgggcag cgtgctcggc cacgagatga tggggcgggt gctcgcgctc
      181 ggccagggcg tcgaggcgga ttacgccagc gccccgctca gggtgggaga ccgcgtggtg
      241 tgcccctact tcatcacgtg ccggaggtgt tcggcctgcc aggccggatt gttcaacctc
      301 tgcgccaact gctaccgcta ctggtcgcag ccggccgacg ccccgccgca cttccacggc
      361 gcatttgcca cgcactacta cgtccacccc gaccagtacc tgtacaaggt gccggatccg
      421 gtccccgacg ccgcggccgc cagcgccaac tgcgcgctgt cggagctgct cttcgggctc
      481 gaccaggcgg gggtggccgc aggcgagcag gtggtggtcc agggcgccgg gggcctcggt
      541 ctctgcgcgc tcgcggtggc caaggcacgg ggggcccgaa cgattgtgat cgatggggta
      601 ccgcggcggc tcgagctggc ccaccggttc ggcgccgatc tggtcgtaga cctgcgtgac
      661 gacgacaccc ccgagcggcg ggcggcccgc atccaggcgc tcaccggtgg gatgggcgcc
      721 gacgtggcgg tggaggtgac gggcgtgccg gcggcattcg ccgagtcgat ccacctcgtc
      781 cgcccgggcg ggcgcgtcgt ggagctcggc aacatctccc ccggccacac caccccgttc
      841 gatccgggca ccctcacccg ccgcggcgtc cagatccttg ggacgatccg ctaccacccc
      901 tggtatctca agcgggccct ggacttcctc gtggcgacgc ggggccgctt cccgttcgag
      961 gcgctgctgg acgccgagta cccgctccgc gacgtggggc gggcgctcga ggactccgac
     1021 cagcggaaag tcacgcgcgc ttcgctcctc gtgggacgct gaggggaacc gcagggatcg
     1081 ccgaaaggaa tccgatcgag gccgccggtc cgggctacgg ggaggacggg acccccggcc
     1141 gcaggcacgc ctccagcgcc cccagcaggc gtcgaatcgt ctcgggggtg gcttggttgc
     1201 ccaggtgtcc gatgcgaatc acccggcccg tgagatgctc caggcccccg gccacggcga
     1261 tccggtgctg aatgcgcagc gtctccatca aggcgctggc tttgacgccc tccgggggat
     1321 aggccacggt gacggtaggc gacgcccacc ggcggtcgac gaacaccttg aatccgaggc
     1381 ggtcgagccc ctcgcgcaac tgcgcggccg cccggcggtg gcgctcaaac cggacctcca
     1441 acccctcggc aaagatccgg tcgagtgccc ggtcgagggc gacgatgttg ttggccgcga
     1501 gcgtgaccgg gtagggatga ttggcccact cacgctcgta ctgccgccac gtg
//
LOCUS       DASQGH010000257         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9566, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000257 DASQGH010000000
VERSION     DASQGH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9566"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VEW91_04985"
     CDS             complement(<1..813)
                     /locus_tag="VEW91_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90972.1"
                     /translation="MRRTQAGFVTLAALLIILIITATGAILLTSLFGEIQGESGYNQA
                     TGALALGEAGLHWAGNKLTGPSAVTEVYAGDSNQAVQGAGGQPVGVFDVTVTCANGAA
                     VSTGCAVQPGQRLVRATGYVPSKTTALGQRTVQALVAQNTFYNKTICAYTNVNLDQGV
                     TVQGDVGSEGTTIPDLSLQGPLSNPARIQPAPGNAQPGNAYAVGTVSCSVGCSTQVAG
                     TENQNQTPGTVCPNRTQVSTSYTCVPGSGTLSGGPVTISSSNNSLSAVTLGAG"
     gene            complement(824..1384)
                     /locus_tag="VEW91_04990"
     CDS             complement(824..1384)
                     /locus_tag="VEW91_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90973.1"
                     /translation="MTLLEAVAALAITTLAFGSVHLTVGTAVLGGLLTSSTVAKQQQG
                     RQVVEWITDRVRQAGFRASGSPIPRCQDRIATVSPYLPTASSLWVNGDVDNTGTPETL
                     GFQIETVSGTSAVTETVINCVTGAVTQDQPITSPTSVKALALGFGYYDASGNAVTNLS
                     NPAAIRTIRFVTVTVQVQASVDLRNP"
     gene            complement(1431..1859)
                     /locus_tag="VEW91_04995"
     CDS             complement(1431..1859)
                     /locus_tag="VEW91_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90974.1"
                     /translation="MTRSRARHGRERGIGLVDVMIGLTVLAFVTASLLSVFLSSIAQA
                     HASGARAEAATWVQGEADYLRWMGYTSSCLAAGTRTLTPTSAGCTALEPTLPREFAQA
                     TVQVENNVLGQTGLKRITIEVSQVPGTVFYRVVTYETQFL"
     gene            complement(1856..>2279)
                     /locus_tag="VEW91_05000"
     CDS             complement(1856..>2279)
                     /locus_tag="VEW91_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYM90975.1"
                     /translation="PVRERGVTVLETMVAVAVFALITGAAVASVRGFLSQKSLAGWSD
                     SIVNDIRGAQQLAIARRAAAVVTFTNGAPASYTTQVGGATVRSQTLPVPLTITSSSIQ
                     FNTLGVPASGVTLSLTDSRTGKTITISVAPVTGAVTAQ"
BASE COUNT          330 a          787 c          778 g          384 t
ORIGIN      
        1 gccggcgccg agcgtcacgg cgctgagcga gttattgctg ctggagatcg tgaccggccc
       61 tccgctgagg gttccgctcc cgggcacgca ggtgtaggag gtcgagacct gtgtccggtt
      121 cgggcacacc gtgccgggcg tctgattctg gttctcggtg ccggcgacct gggtgctgca
      181 gcccaccgag cagctgaccg tgccgacggc gtaggcgttc cccggctggg cgtttcccgg
      241 agccggctgg atccgcgcgg ggttgctcag cggtccctgc aggctgaggt ctgggatggt
      301 cgttccctcg ctccccacat cgccctgaac ggtgaccccc tggtcgaggt tgacgttcgt
      361 gtacgcgcag atcgtcttgt tatagaaggt attctgggcc accagggcct ggaccgttcg
      421 ctggcccagc gccgtcgtct tggacgggac atagccggtc gcgcgcacga ggcgctggcc
      481 gggctggacc gcgcacccgg tgctgacggc cgcgccgttc gcgcacgtca cggtgacgtc
      541 gaacaccccc accggttggc cgcccgcccc ctgaacggcc tggttgctgt cgccggcgta
      601 cacctcggtc accgccgagg gccccgtcag cttgttcccg gcccagtgca acccggcttc
      661 tcccaaggcc agggcgccgg tcgcctggtt gtacccggac tctccctgga tctccccgaa
      721 gaggctggtc agcagaatgg cgcccgtcgc ggtaatgatc aggatgatca ggagcgccgc
      781 aagggtaacg aatcccgcct gggtccgacg catgggtccc ctcctatgga tttcgcaggt
      841 cgacgctcgc ctgcacctgg accgtcacgg tgacgaagcg gatggtcctg atcgccgccg
      901 ggttgctcag gttggtcacc gcgtttccgc tcgcgtcgta gtagccaaac ccgagcgcga
      961 gggccttgac cgaggtgggc gaggtgatgg gctgatcctg ggtgaccgcg cctgtgacgc
     1021 agttgatcac cgtctcggtc acggcgctcg tcccggagac ggtttcgatc tggaatccca
     1081 gggtctccgg ggtcccggtg ttatcgacgt ccccgttgac ccacagcgac gatgccgtcg
     1141 gcagatacgg ggagaccgtg gcgatcctat cctggcaccg cgggatcgga ctgccggaag
     1201 cgcggaaccc ggcctgccgg acccggtcgg tgatccactc gacgacctgc cgtccctgct
     1261 gctgcttcgc gaccgtgctc gatgtcaaca gcccgccgag caccgcggtc ccgacggtca
     1321 ggtgcaccga gccgaacgcc agcgtggtga tcgcgagggc cgcgaccgcc tcgagcaacg
     1381 tcatgccggc ctcgcctcgc gcccgccggg tctgagcaag actcgcaagc ctacaggaac
     1441 tgcgtctcat aggtcaccac cctgtagaac acggttccgg gcacctggga tacctcgatc
     1501 gtgatccgct tgagcccggt ctgaccgagc acgttgtttt ccacctgcac ggtggcctgg
     1561 gcgaactccc ggggcagcgt gggttcgagc gcggtgcacc cggccgaggt gggcgtgagc
     1621 gtgcgggtgc cggcggcgag gcacgagctc gtgtacccca tccaccgcag gtagtccgcc
     1681 tctccctgga cccatgtggc cgcctccgcc cgggcccccg aggcgtgggc ctgcgcgatg
     1741 ctcgagagga aaacgctgag gagggaggcc gtgacaaacg ccagcaccgt gagcccgatc
     1801 atcacgtcga ccaggccgat gccgcgctcc cgcccgtggc gggcacgaga tcgggtcatt
     1861 gggccgtcac cgccccggtc accggcgcca cggagatcgt gatcgtcttc ccggttcggc
     1921 tatcggtgag gctgagcgtc acccccgatg cgggcacacc gagcgtattg aactggatgc
     1981 tcgaggaggt gatggtgagc ggtacaggga gcgtttggct ccgcaccgtc gctccgccga
     2041 cctgggtggt gtagcttgcc ggagccccgt tcgtaaaggt caccaccgcg gccgcccgcc
     2101 gggcgatggc caactgctgg gccccgcgga tgtcgttcac gatcgagtcg ctccatccgg
     2161 ccagcgactt ttgggagagg aacccacgca cggacgccac cgcggcgccc gtgatgagtg
     2221 cgaacaccgc caccgccacc atggtttcca gcacggtgac cccgcgctcg cgcaccgga
//
LOCUS       DASQGH010000258         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1129, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000258 DASQGH010000000
VERSION     DASQGH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VEW91_05005"
     CDS             complement(<1..181)
                     /locus_tag="VEW91_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90976.1"
                     /translation="MVTVGSGGYVFEVVEDWARLPEGWTFHEVSGVGVDHHDRVYVFS
                     RGKHPLTVFDRDGTFV"
     gene            complement(309..929)
                     /locus_tag="VEW91_05010"
     CDS             complement(309..929)
                     /locus_tag="VEW91_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM90977.1"
                     /translation="MSDLRVIVKSADALARAGLSALLGAHPGYVVVGENGTDPELPDS
                     PDAGRADVIVWEVGADPRAHPDDLAELADGAVPVVALLPDEMPAGLALAAGARGVLPR
                     DVDADCLAACLQAVMQGLLVVHPEFAASLPVRERAPALVEELTPREREVLQLLAEGLP
                     NKVIADRLQITEHTAKYHVAAILAKLGAQTRTEAVARAARLGLILF"
     gene            complement(1034..>1775)
                     /locus_tag="VEW91_05015"
     CDS             complement(1034..>1775)
                     /locus_tag="VEW91_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYM90978.1"
                     /translation="EREDGITVGLPGGDAVPASLVGRDPRTDVAVLRAQATGLAAAPW
                     APPDDLRVGHLLVAVGRPGRTVRAAFGFVSALGPMWAARAGAEIDTYLEADLTAVPGF
                     SGGVLLDATGKARALTTTGLTPRGISGVTLPTLRRVVDVLLTYGRIRRAFLGVGAQPV
                     HLPVSLAGRANQEEGLLIIGVEPESPAERAGLLLGDTIVALDGKPVRGVRELLAQLGE
                     AQIGTSVPVRILRGGEVQDRTVLVGERR"
BASE COUNT          267 a          662 c          624 g          222 t
ORIGIN      
        1 ccacgaacgt gccgtcgcgg tcgaagaccg tgagcgggtg cttgccgcgg ctgaacacat
       61 agacccgatc gtgatggtcg accccgacgc cggacacctc atggaaggtc cacccctcgg
      121 gcagtctggc ccagtcctct acgacctcga agacgtaccc cccactaccg acggtgacca
      181 cacctcacct cgcgcgcacg aattctgatc ctcgattcca ccgcgccgcc gggctcccct
      241 gctcgcgcag gcgtgcagag cggaccccga gtccgcggtc ggactttggg cttcgcggcg
      301 acgggctgct agaagaggat caaccccagc cgggctgcgc gcgcgaccgc ctcggtccgg
      361 gtctgggcgc caagcttggc aaggatcgcg gccacatggt acttggcggt gtgttcggtg
      421 atctgcaggc ggtcggcgat gaccttgttc ggcaatccct cggccaggag ctgcagcacc
      481 tcccgctcgc gcggcgtcag ctcctccacg agcgcgggcg ctcgctcccg aacggggagg
      541 gacgcggcga actccgggtg gaccacgagg agcccctgca tcaccgcctg gagacaggca
      601 gccagacagt ccgcgtcgac atcgcgggga aggaccccgc gggctcccgc ggccagcgcc
      661 aggcccgcgg gcatctcgtc cgggaggagc gcgacgacag gcacggcacc gtcggccagc
      721 tcggcgagat cgtcggggtg cgcccgcgga tccgcgccga cctcccacac gatgacgtcg
      781 gcccggccgg cgtcggggct gtcgggtagc tccgggtccg tcccgttctc accgacgacc
      841 acatagccgg ggtgcgcacc caagagagcc gacagccccg ctcgggcgag ggcatcggct
      901 gacttcacga taacgcggag gtcggacatg aggggggcac ggaggaccga gggcctcgtg
      961 gcgtctgatg cgggaacgcc tgcatggccg gggcgattcc ccagggccgc ggcgggccgg
     1021 tggcggcacc cccctatcgg cgctcgccga cgagaacggt gcgatcctgg acctccccgc
     1081 cccgcaggat ccgcaccggc accgaggtgc cgatctgcgc ctccccgagc tgggccagga
     1141 gttcgcggac gcccctgacc ggctttccgt ccagcgcgac gatcgtgtcg ccaaggagca
     1201 gcccggcgcg ctccgccggg ctttccggct cgaccccgat gatcagcagc ccctcctcct
     1261 ggttcgcgcg gccggccagg gacaccggga ggtggaccgg ctgcgcgccg accccaagga
     1321 acgcccggcg gatccgtcca taggtgagca ggacatccac gacccggcgg agcgttggca
     1381 gggtcacgcc gctgatcccc cgcggcgtca gccctgtggt cgtgagcgcg cgggccttcc
     1441 cggtggcatc gagaagtacc ccccccgaga accccgggac cgcggtgaga tcggcctcga
     1501 ggtacgtgtc gatctcggca ccggcccggg cggcccacat cgggccaagc gcgctgacga
     1561 acccgaacgc cgcccggacc gtccggccgg gccggccaac cgccaccagc aggtgtccga
     1621 cccggaggtc atcggggggc gcccacgggg ccgcggcgag gccggtggcc tgagcccgca
     1681 ggaccgcgac gtccgtcctg ggatcgcgcc caacgaggga tgccgggaca gcgtcgccgc
     1741 cgggtaagcc gacggtgatg ccatcctccc gttca
//
LOCUS       DASQGH010000259         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46486, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000259 DASQGH010000000
VERSION     DASQGH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46486"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="argJ"
                     /locus_tag="VEW91_05020"
     CDS             complement(<1..1198)
                     /gene="argJ"
                     /locus_tag="VEW91_05020"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HYM90979.1"
                     /translation="MGDGVTRLIQGGVTSAQGFVASGVHCGIKPQGKDLALVVSRVPA
                     AAAGVFTTNKVKAAPVLLDMERIRSGQGRAVLLSSGNANACTGEQGLRDAREMAHLVA
                     EHLQVPEDLVYVCSTGQIGVPLPMEAIRRGVPEAVRLLGPDGTAAAEAILTTDTGPKA
                     GAVQVMIGQQVVTVGGMSKGAGMIHPQMATTLTVLTTDAAVAPAVLQAVLRRAADQSF
                     NRITVDGDRSTNDTILVLANGEAGAPEVTGPGEALDALQAALNAVTVRLAKAVAQDGE
                     GATKLVEITVEGAQTDEDAERAAFAVANSPLVKTAIHGAGANWGRIMAAVGYSGAEVQ
                     PDRVTVRIGPVVVAERGTLAGGPERLEQAGEHLMGQDVRVGVDLGLGRGIATVWTCDL
                     SEEYVKE"
     gene            complement(1212..2258)
                     /gene="argC"
                     /locus_tag="VEW91_05025"
     CDS             complement(1212..2258)
                     /gene="argC"
                     /locus_tag="VEW91_05025"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HYM90980.1"
                     /translation="MPVRVSVIGASGYGGAEAVRLLAVHPEVRLAHVTADIQTNRPLA
                     SLYPNLRGFVDLVTEALDPAAIGRDSDLTIVSLPSGKAMEIVPELLERGTRVIDVAAD
                     FRLKDPAQYPVWYNFAHTAPQYLAEAVYGLPELHREAIRSARLVANPGCFPAAALIAL
                     APLVRAGAIRREGIVIDAKSGVSGAGRGGRSGGYAFADTNEDVRAYSVPGHSHTAEIE
                     QELSGHAGAGVRVTFVPHLIPMTRGILVTAYGRLARAITTLDAMAVYREAYAGEPFVR
                     VLPDGVLPQTKATTGSNFCDIAVRIDPRAEVVVAIAALDNLGKGAAGQAVQNMNLMLG
                     FPEDAGLRVPALYP"
BASE COUNT          358 a          955 c          839 g          409 t
ORIGIN      
        1 tttccttgac gtactcctct gagagatcgc aggtccacac ggtcgcgata ccccggccca
       61 gcccgagatc aaccccgacc cgaacgtcct gccccatgag atgctcgccc gcctgctcga
      121 ggcgctccgg gccgccggcc agggtgccgc gctcggccac gacgaccgga ccgatgcgga
      181 ccgtcacccg atcgggctgg acctcggccc ccgagtaccc gaccgccgcc atgatgcgtc
      241 cccagttggc ccccgcgcca tggatcgccg tcttgaccag cggggagttg gccaccgcaa
      301 acgcggcgcg ctccgcgtcc tcgtcggtct gcgcgccctc caccgtgatc tcgacgagtt
      361 tcgtggcgcc ttccccatcc tgggccaccg ccttggccaa ccgcaccgtc acggcgttga
      421 gcgcagcctg gagcgcatcc agcgcctctc ccggaccggt gacctcaggg gccccggctt
      481 ccccgttggc gagcacgagg atcgtgtcgt tcgtgctccg gtccccatcc accgtgatgc
      541 ggttgaacga ctggtcggcc gcccgtcgga ggaccgcttg gaggaccgcc ggagccaccg
      601 ccgcgtcggt cgtcaggacg gtgagggtgg tcgccatctg cggatggatc atgccggccc
      661 ccttgctcat cccgcccacg gtcacgacct gctggccgat catgacctgc accgccccgg
      721 ccttgggccc ggtatcggtg gtcaggatgg cctccgcggc tgccgtccca tcggggccca
      781 ggaggcggac cgcttcgggg acgccgcgcc ggatcgcctc catcggcagg ggcaccccga
      841 tctggccggt gctgcacacg tacacgaggt cctcggggac ctggaggtgc tccgccacca
      901 ggtgggccat ctcgcgagca tcccgcaacc cctgctcgcc ggtgcaggcg ttcgcgttgc
      961 cgctgctgag cagcacggcg cgtccctgcc cggatcggat ccgctccatg tcgagcagca
     1021 caggcgcggc cttgaccttg ttggtcgtga acaccccggc cgccgcggcc ggcacccgcg
     1081 agaccaccag ggccaggtcc ttcccctgcg gtttgatccc gcagtggacc ccggaggcga
     1141 cgaacccctg cgccgaggtc acccctccct ggatcaaccg cgtcacgccg tcgcccatgg
     1201 cccggcctcc tttatggata gagggcgggc acccggagcc ccgcgtcctc gggaaagccc
     1261 agcatcaggt tcatgttctg gacggcctgg ccggccgcgc ccttgccgag gttgtcgagc
     1321 gccgcgatcg caacgaccac ctcggcgcgg gggtcgatgc gcacggcgat atcgcagaag
     1381 ttgctccccg tcgtcgcctt cgtctgcggg agcacgccgt ccggcagcac gcgcacgaac
     1441 ggctccccag cgtaggcctc ccggtacacc gccatcgcgt ccagcgtggt gatggcgcgg
     1501 gccagccggc cgtaggccgt cacgaggatg ccccgggtca tcgggatcag gtgcggcaca
     1561 aacgtcacgc ggactccggc gcccgcgtgg cccgagagct cctgctcgat ctccgcggtg
     1621 tggctgtgcc ccggcacgct gtaggcgcgc acatcctcgt tggtgtcggc gaacgcgtac
     1681 ccgcccgacc tgcccccacg gccagccccc gacacccccg acttcgcgtc gatcacgatg
     1741 ccctcgcggc ggattgctcc cgcgcggacg agcggcgcca gggcgatcag cgccgcggcc
     1801 gggaagcagc cgggattggc gacgaggcgg gcgctccgga tcgcctcccg gtgcagctcc
     1861 ggcaggccgt acacggcctc cgccaggtac tgcggcgcgg tgtgcgcgaa attgtaccag
     1921 acggggtact gggccgggtc tttcagtcga aagtccgccg ccacgtcgat caccctggtc
     1981 ccccgctcca ggagttcggg gacgatctcc atggccttgc cgctcggcag cgagacgatc
     2041 gtcaggtcgg agtctctccc gatcgccgcg ggatcgagcg cctcggtgac caggtcgacg
     2101 aacccgcgga ggttggggta gaggctggcc aggggccggt tcgtctggat gtcggcggtc
     2161 acgtgcgcga gacggacctc ggggtgcacg gccagcaggc gcaccgcctc cgccccaccg
     2221 taccccgacg ccccgatcac gctgacccga accggcatct gcgcttgcct ccgtgccgcc
     2281 cgcccggacg gcggatccgc ctcgctccaa tcgatgcggt ataattatac tgtcttatga
     2341 ataaacttgc aatgggggcg gcccggggta gggacgcgcg tcccggggcc aaaggccggt
     2401 gctccgggcg acgatcgacc tcggacccaa cgtctacccc gattttttct tctaaggaaa
     2461 agaaaacttt ttgttgggct ctgctctgac cgagcaaggc ggcggggctg gctgctattt
     2521 ttgctcggac gcccgtctta gcgtctcaat gtccagcttt t
//
LOCUS       DASQGH010000260         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18187, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000260 DASQGH010000000
VERSION     DASQGH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18187"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VEW91_05030"
     CDS             <1..624
                     /locus_tag="VEW91_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90981.1"
                     /translation="NTPDDAYQLPDGRITVADIKNCRILLFDPPPAHHIQKQFGVTKD
                     CRAAPGHFLYPNGDTPLPNGHILVTEIGTRSASELDPATGQILYRVPLHVAYPSDTQL
                     TRDGQYLVVDYSRPGQIVVVNRSGQITWSYGPRSGPGRLDHPSLALELPGGDFLLNDD
                     LNHRVIVLSRSTKQILWQYGVTGTAGRAPGYLHTPDGVDIKPATFGR"
     gene            complement(648..1220)
                     /locus_tag="VEW91_05035"
     CDS             complement(648..1220)
                     /locus_tag="VEW91_05035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYM90982.1"
                     /translation="MGVPARTRWGASAALLLPLIAVLGFLGWATLRHQQSLQIGAALA
                     RGEMPPAPSVALPAFDGRKVSLEDLRGHPVILNFWASWCVPCREEAPILEAIWGEFRT
                     RGVIVVGVDTQDLETPARRFLTQHGITYLNVRDPDGHVAHLFGTTGVPETFFIGRDGR
                     IRGKFPGEQPDRSVWRAAAAALLAGSGRIP"
     gene            1710..>2075
                     /locus_tag="VEW91_05040"
     CDS             1710..>2075
                     /locus_tag="VEW91_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HYM90983.1"
                     /translation="MPAKLLLYDEEARKALERGVEKVASAVRVTLGPKGRNVVLEKKW
                     GSPTITKDGVTVAKEIELEDPYENMGAQLVKEVASKTNDAAGDGTTTATVLAHAIVRE
                     GLKNVAAGANPMILKRGIDK"
BASE COUNT          410 a          715 c          645 g          305 t
ORIGIN      
        1 aataccccag acgatgccta ccagctcccg gacggccgga tcaccgttgc cgacatcaag
       61 aactgtcgca tcttgctctt cgatcctccg cccgcccatc atatccagaa gcaattcggc
      121 gtgaccaagg actgccgggc cgcccccggg cacttcctat acccgaacgg ggacaccccg
      181 cttcccaacg gccacattct cgtcacagag atcgggaccc gcagcgcctc agaactcgac
      241 cccgccaccg ggcagatcct ctaccgggtc ccgctccatg tggcgtatcc ctctgacacg
      301 caactcacgc gcgacggcca gtatcttgtg gtcgactaca gccgcccggg acagatcgtc
      361 gtcgtgaacc gatcgggaca gatcacgtgg tcgtatggtc cgcgatccgg ccccggccgg
      421 ctcgaccacc cctcgctcgc gctcgagctg cccggcgggg acttcctcct caatgacgac
      481 ctgaaccaca gggtgatcgt cctgagccgc agcaccaagc agatcctctg gcagtacggg
      541 gtcacaggga ccgccggccg ggctcccggc tacctgcaca cgcccgacgg ggtcgacatc
      601 aagccggcaa cctttggccg gtagcgccgg atgtggaggt cctcggctta ggggatgcgg
      661 ccgctcccgg cgagcagcgc ggctgcggcc gcgcgccaca cggaccggtc tggttgctct
      721 ccggggaact tcccgcgaat ccggccgtcc cggccgatga agaaggtttc cgggaccccc
      781 gtcgtcccaa acagatgtgc gacatgccca tccggatcgc gcacattgag gtaggtgatg
      841 ccgtgctggg tgagaaaacg gcgcgcgggg gtctcaagat cctgggtgtc gaccccgacc
      901 acgatcaccc ccctggtccg aaactcgccc cagatggctt cgaggatcgg ggcctcctcc
      961 cggcacggca cgcaccacga agcccaaaag ttgaggatca ccgggtgccc ccgaaggtcc
     1021 tccagcgaca ccttccgccc gtcgaacgcc ggcagggcga ccgagggggc cgggggcatc
     1081 tccccgcgcg ccagcgccgc gcctatctgc aacgactgct ggtggcgcag ggtggcccag
     1141 ccgaggaagc caagcaccgc gatcaggggc agcaacagcg cggccgaagc gccccagcgg
     1201 gtgcgggcgg ggactcccat gccccgatcg taccagaaaa gggctgcggg ggaggcctcg
     1261 acgcgctccc ccgtggccga tcttccctaa aacttgcctt gaccacgccc gatcgccgtg
     1321 ctaaggttaa cccccgtctc aacgaggggt ctgcaaggcc gggacgccac ctcaagtaac
     1381 tgcggcaggc gatccggccc aagagcagca accttctcca gggcgggcga gccggcggct
     1441 gcgacagccc aggacagaac tctctgtact gggcggcagc cgtcgctgct taccagcgat
     1501 ctgcgccacg ggatccgcgt aatcgcttga tttcgctaga tatcgacaga aacctcacgt
     1561 aatgctacga taaattatgg aattgggccc aggggcggat tcatccccga atggtatcct
     1621 tgaaagccgg tccatgccca tgaaaaatgg atgtggttag cactttcacc gggtgagtgc
     1681 taaacttcac agatcgagga ggctatcgca tgcccgccaa gttgttgctc tacgatgagg
     1741 aagcccgcaa ggccctcgag cgcggcgtcg aaaaggttgc cagcgcggtc cgcgtcacgc
     1801 tcggccccaa agggcggaac gtcgtgctcg agaagaagtg gggctcgccg acgatcacca
     1861 aggacggcgt caccgtcgcc aaggagatcg agctcgagga cccctacgag aacatggggg
     1921 cgcaactcgt caaagaggtg gcgagcaaga ccaacgacgc cgcgggcgac gggacgacca
     1981 cagccaccgt gctggcccat gcgatcgtca gggaaggcct gaagaacgtg gccgcgggcg
     2041 ccaacccgat gatcctgaag cgcgggatcg acaag
//
LOCUS       DASQGH010000261         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4016, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000261 DASQGH010000000
VERSION     DASQGH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4016"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VEW91_05045"
     CDS             complement(<1..764)
                     /locus_tag="VEW91_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM90984.1"
                     /translation="MADDEAGLPAEISGQLDVYHHALHLAEQNAILDAAVWFHTGLSH
                     RVAGRGDSAVICFKKVIEVDPDEPLAYWELVDLYIQQEAPEAAVIVLSALGERLAGRL
                     VWTQAVAAWKRAAELAPDDADILRALQEAYAHAGQIQEAAAIQSILQALRPGMPAPAQ
                     PAPDAASAAPTAAPTLPASPPTPADGSAGARPATDGPAPAKPAAGGKAEGGTRKGSHP
                     RGVGSSSSAGSQSPARGPGHRLADSGKGRRSREPLLG"
     gene            complement(785..1156)
                     /locus_tag="VEW91_05050"
     CDS             complement(785..1156)
                     /locus_tag="VEW91_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HYM90985.1"
                     /translation="MTTAAAVGLKEVLGDLTKKPGVIAALVVSRDGFVIEGLTSEEEL
                     DLDALGAHTSSALMGWESIGTDLSRGAPEMLLVEFESGPISVSPVSGDAVLVVLGNRL
                     CNLGRLRIEASRVREAVAACL"
     gene            complement(1194..1568)
                     /locus_tag="VEW91_05055"
     CDS             complement(1194..1568)
                     /locus_tag="VEW91_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90986.1"
                     /translation="MKKAREVLAAMVAGTEGVLAAGLIGMDGAPIEVLKLEAEFPAES
                     ISQDVVTAVKLFLYMSRKVEGGSLDHIMMSCERFMVLMAVPGLDHCVALFLTSKGNLG
                     KARVQFRRYLPDLIGAVEGATG"
     gene            complement(1897..2718)
                     /locus_tag="VEW91_05060"
     CDS             complement(1897..2718)
                     /locus_tag="VEW91_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM90987.1"
                     /translation="MAVREPRSTAPADDQDLERLEMFRRYRRTADPELRQDLVCHYLP
                     LARKIARRYVRAGVPLDDLTQIGTIGLMNAVSSFDPGRGVKFEAYAYHHVAGEIRHYL
                     RDSVEQVRAPRWVRKLYGELTDTVAELRQTLGRTPTLAEIAGRMNLTQAGVLEILRAH
                     NRSRVHSMSDLVDRQEIRRDVIAHQRYISFQLPVEDRIVLLKAVERLADLQRKVVYYL
                     FYQDLTQSEVAKRLGVSQRHVSRVLAAALKRLAVHLRTGEGEPRVDSAAEEVSEP"
     regulatory      complement(2887..2974)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3306..4451
                     /locus_tag="VEW91_05065"
     CDS             3306..4451
                     /locus_tag="VEW91_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM90988.1"
                     /translation="MSGDTHPPAGTPDRGAIQTSIREAAQKAQELQRQAEAARAVLNE
                     AAAKVHELERQAAAAQKTIMRYQEQESEVARAIQDAQASYDEIIQSAKARAEEMVETA
                     KSSADGIVRSARNTATDLLHKARESAQEQLQAAERMATERRALLQTGPNEAGDADPEA
                     EETKKAAEEYLTGVIDKLDTFIRDRDGVSRSLEAAATQYAESLETVSRLRTEVQREIL
                     PAVNQLLRRLKGGPADDGEAEREPEAEAEDDTERLMPLVSTPASPKPERPTAPDRAPR
                     EQRERPTTAPAPAGHQMGEVVVSPIHSFLQATKFITALSQIQGVTSVKLKSYAGSKLT
                     LDVLTAGYPVAAIDCALIDGFAVEVAESTDTQLVLRLGSPAARPVSR"
BASE COUNT          749 a         1576 c         1480 g          666 t
ORIGIN      
        1 ccgaggagcg gctcgcgcga tctccttccc ttgccgctgt cggccaggcg gtggccgggc
       61 ccgcgcgccg gggactggga gccggccgac gatgacgacc cgaccccgcg ggggtgcgac
      121 cccttgcggg ttcccccttc cgccttcccg cccgcggcgg gctttgcggg agcgggccca
      181 tcggtcgccg gccgtgcccc ggcggaccca tccgcggggg tgggggggga ggccggaagc
      241 gtcggggcgg cggtgggcgc ggcggaagcg gcatcagggg ccggctgcgc cggggccggc
      301 atgccgggcc tgagggcctg caggatcgac tggatggcgg cggcttcctg gatctgcccg
      361 gcgtgcgcgt aggcctcctg gagcgccctc aagatgtcgg catcatcggg ggcgagctcg
      421 gccgcgcgct tccacgcggc gacggcctgg gtccaaacca ggcggcccgc cagccgctcc
      481 cccagcgcgc tcagcacgat gacggcagcc tcgggagcct cctgctggat gtagagatcg
      541 acaagctccc aatatgccag gggctcgtcg ggatccacct cgatgacctt cttgaagcag
      601 atgaccgcgc tgtccccccg ccccgccacc cggtgcgaca gccccgtgtg aaaccacacg
      661 gcggcgtcaa ggatggcgtt ctgctcggcc aggtggagcg cgtggtggta gacgtcgagt
      721 tgcccggaga tctcggctgg aagcccggcc tcgtcgtccg ccattgtgct tgagcgagga
      781 tcgctcacaa gcaggccgcg acagcctcgc ggacccggct cgcctcgatg cgcagccgcc
      841 cgaggttgca gaggcggttg cccaacacga cgagcacggc gtcgccggag acgggggaca
      901 ccgagatcgg tccggactcg aactcgacga gcagcatctc gggggctccc ctcgacaggt
      961 cggtgccgat ggattcccac cccatcaggg cgctgctcgt gtgcgcgccc agcgcatcga
     1021 ggtccagttc ctcctcggag gtcaaccctt caatcacgaa gccgtcgcgg ctcaccacca
     1081 aggccgcgat gacccccggc ttcttcgtga gatcgccgag gacttctttg agacctaccg
     1141 ctgctgccgt cgtcacggct gcacctcctg ctcgttgctc gggcccgcct cgcctatcct
     1201 gtcgcgccct caacggcccc aatcaggtca ggcaggtacc ggcggaactg cacacgcgcc
     1261 ttcccgagat tccccttgct cgtgagaaag agcgccacgc aatgatcgag gcccgggacc
     1321 gccatcagca ccatgaaccg ctcgcagctc atcatgatgt ggtccaggga ccccccttcg
     1381 acctttcgcg acatgtagag gaagagcttg accgccgtca cgacgtcctg gctgatgctc
     1441 tccgccggaa attcggcttc cagcttgagg acttcgatcg gtgcgccatc cattccgatc
     1501 agtcctgctg ccaagacccc ttcggtcccg gccaccatgg ccgcgaggac ctcgcgggcc
     1561 ttcttcattc accccgcccc cccccgatgt tggagccggc cgcagcggct ggctcggcgg
     1621 atcgcgccga gggcccggcg gcggcctcgt ccaaatccgg ctgcgccgcg agccacgccc
     1681 tgacgccctg cagactcatg ccctcctttt caaccaaccg acggatgcac tgaacgagac
     1741 ggagatcccg ggtcgagtac agccggtgcc cgcccttggt ccgggcaggc cgcaggagct
     1801 ggtactccac ctcccaactt cgcagtcgcc tcgcatccac cccagacagc gatgcgacgg
     1861 tccgaatggg gtaaatgggc agatcgtccg acgtgttcac ggctcgctga cctcctccgc
     1921 ggcgctatcc acccggggct caccctctcc cgttcgcagg tgcacggcca agcgtttgag
     1981 cgcggcggcg agcacccggg acacgtgccg ctgggagacc cccaggcgct tggccacctc
     2041 gctctgcgtg agatcctggt agaacaggta gtaaacgacc ttccgctgca ggtcggccaa
     2101 ccgctccacc gccttcagca gcacgatccg gtcctcgacc ggcagctgga acgagatgta
     2161 gcgctggtgc gcgatgacgt cccgccggat ctcctggcgg tcgacgaggt cgctcatcga
     2221 atgcacccgg gaccggttgt gggcgcgcag gatctcgagc acgccggcct gggtcagatt
     2281 catgcgtccg gcgatctccg caagcgtcgg ggtccggccc agcgtctggc gaagctcggc
     2341 gaccgtatcg gtcagttccc catagagctt ccgcacccag cgcggcgctc ggacctgctc
     2401 gacgctgtcg cgcaggtagt gccggatctc ccccgcgacg tggtggtagg catacgcctc
     2461 gaacttgacg ccccgcccgg gatcgaatga actcaccgca ttcatgaggc cgatggtgcc
     2521 gatctgggtc aggtcatcga gcggaacgcc ggcccggacg taccggcgcg cgatcttcct
     2581 ggccagcggc aggtaatggc agacgaggtc ctggcgtaac tccggatcgg cggtgcgccg
     2641 gtaccggcga aacatctcaa ggcgttcgag gtcctggtcg tccgctggag cagtcgagcg
     2701 gggttccctg actgccaacc ggctgctgcc catcgttccc tccctacgcg gccgcgcggc
     2761 ttcgtgtcac ccgcgacggc gctgccctga tcccgcgaca ccgggggcgg tcaggagggg
     2821 gacgggcgac aggaggtgtg gaacagtaag acctcccgcc taccacggga ggtcaggagt
     2881 agccgctccg gcgacccggc cgactggccc agccctgctg gccagccccg tggctttgcg
     2941 tccccagatc tctctgggtt tgcccctacg gaagcgcgat atcaaatgct atggagttat
     3001 tagcatcaga gtacaccaca cagcatggtg gctgtcaagg aattccagag agagtggttg
     3061 gatgatgcgc gcaggttccc cgtattccgt cgcacctctc ccggaccgcc atcaaaccgc
     3121 cagggatctg ccgggcgcct accctggaaa cgtctaatcg gggggcgttg tgggcattgg
     3181 gtgaacagac agagatccct cgggagcacc tcaggaggaa ttgccgtgcg ccatcccgcc
     3241 cggccccggg cgagtgtgtc ctgcacagca cgtctgcaac aaacccaagg taaggaggtt
     3301 gcgggatgag cggtgacacc cacccgcctg ccggtacccc cgatcggggg gcgatccaga
     3361 cgtcgattcg agaggccgct cagaaagccc aggagctgca gcgccaagcg gaggccgccc
     3421 gggccgtcct aaacgaggcc gccgcaaagg tgcatgagct ggagcggcag gcagcggcag
     3481 cgcagaagac gatcatgcgc taccaggagc aagaaagcga ggtcgcgcgc gcgattcagg
     3541 acgcgcaggc gtcctacgac gagatcatcc agtccgccaa agcgcgcgca gaggaaatgg
     3601 tcgagaccgc gaagtcgtcc gccgacggga tcgtccggtc cgcgcgcaac accgccacag
     3661 acctgctgca caaggcccgg gagtcggccc aagagcagct gcaggcggcc gagcgcatgg
     3721 cgaccgagcg ccgcgcgctg ctccagaccg ggcccaacga ggccggagac gccgaccccg
     3781 aggccgagga gaccaagaag gccgccgagg agtacctcac gggtgtcatc gacaagctcg
     3841 atacgttcat tcgcgaccgg gacggagtgt cccgcagcct tgaggcggcg gcaacgcaat
     3901 acgccgaatc gcttgagacc gtgagtcgcc tcaggacaga agtgcagcgt gagattctcc
     3961 cggcggtgaa ccagctgctg cgccggttga agggcggccc cgccgacgac ggcgaagccg
     4021 aacgcgagcc tgaagccgag gcggaagacg acaccgaacg gctgatgccc ctcgtctcga
     4081 cccctgcgtc cccgaagccc gagcggccca ccgcgccgga cagggcccca cgcgagcagc
     4141 gcgagaggcc cacgaccgcc cccgcccccg cgggccacca gatgggggag gtcgtcgtca
     4201 gcccgatcca ctccttcctg caggcgacca agttcatcac cgcgttgtcg cagatccagg
     4261 gcgtgaccag cgtcaagctc aagagctatg ccggctcaaa gctcactttg gatgtcctga
     4321 cggcgggcta cccggtcgcg gccatcgact gcgcgctcat cgacgggttt gcggtcgagg
     4381 tcgcggagtc gacggacacc cagctcgtcc tgcggctcgg gagcccggct gcccggccgg
     4441 tctccagata gcttgcggag atcttccccc t
//
LOCUS       DASQGH010000262         6859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32396, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000262 DASQGH010000000
VERSION     DASQGH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32396"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VEW91_05070"
     CDS             complement(<1..484)
                     /locus_tag="VEW91_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYM90989.1"
                     /translation="MKRAVTVTVNGVTHTHEVEPRLLLVHYVREVLGLTGTHVGCDTS
                     NCGACTLLMNGKAVKACTLLAVQADGAALQTIEGLAQDGKLHPIQEGFWEEHGLQCGF
                     CTPGMIMTVVDLLQRHPDPTEEQIRWGIEGNLCRCTGYQHIVNAIQHAARTMRGASVK
                     A"
     gene            759..1610
                     /locus_tag="VEW91_05075"
     CDS             759..1610
                     /locus_tag="VEW91_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90990.1"
                     /translation="MEAVLQVLVGGVFTGAEYALAAVGMTLIFGVGRVVNLAQGSFVA
                     LGAYVAYQVTLLGLPAPLGAVPAAAAGLVLGAAVDRIVVRPTRREALTAATVLLGLAI
                     LAEQGFALVWGTSSHSVPLRLPTFLVGRIVVGVEQMAAGAAAAVALGTVALYLRTKPG
                     LALRAAAVDAEIAALGGVDVERIQTATFGVACGMAAAAGALLAPLLTLTPAMGRVPLV
                     LLLAMVAAGGPDRIWGTLVAGFGIGLASTIIGFYLDPAWSYVFALLVLIGAVLWRADG
                     VLGRMRP"
     gene            1607..2545
                     /locus_tag="VEW91_05080"
     CDS             1607..2545
                     /locus_tag="VEW91_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM90991.1"
                     /translation="MTPWRIAWCLLPVGLALAAQDRFILAILALAAVQALYAASWDLA
                     GGVSGQPTLGHALPLGAGAYATALVSGWGLAPPPVAIASGALAGGLIGALQGRLVARL
                     HPVSVALVTLATAECVHEMSGMVRVQWPGGIIVGGDSGIPAVIYPPGETAAAYLTAAV
                     LTAGLVGLLWIAHSGPGLAMRVARTDPRLAAASGIDVARVRMLALTIAGTTAGLAGGL
                     GAALVGRASPSTLALDASLFAMATAGVGGRGSIIGPAAVAYATTAVFQWLDVPATGRL
                     ALYALLLIAAGLVPSAATRLGGDRHTRGGPEGARAK"
     gene            2542..3258
                     /locus_tag="VEW91_05085"
     CDS             2542..3258
                     /locus_tag="VEW91_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM90992.1"
                     /translation="MIRRAGMPAASEPALLRVEDVAKRFGGATALDGLTLSLGAGESL
                     GITGPNGCGKTTLIDVVSGFIRPDRGHVWLAGRDITRWPPHRVVRAGLARTFQQPRLA
                     PRLTVEQILEAAVLHRRLGSSGRRRVVTQILHTVGLADLRRREGWTLPLGAIRRVQVG
                     CALAAGGRLLLLDEPFASLGPEDAPEVVSVLRHLRRDGVSMLIVAHSPALLQALCDRI
                     AVVEAGRVVQTGSPAEVLRA"
     gene            3251..3952
                     /locus_tag="VEW91_05090"
     CDS             3251..3952
                     /locus_tag="VEW91_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM90993.1"
                     /translation="MLEARDLLVRYPRGPVALDGVSLIAHRGEVVALVGPNGSGKSTL
                     LRVIAGLIPPQGGTISLDRAPLTRLAPHQRVARGIAHAPEQARILPTLSVRDNLAIGA
                     WLRRDRRAVARDLERVCEWFPALKERQRGPAEALSGGERQMLALGRVLMAAPRVLLLD
                     EPLTGLDAESRRRIVAVINMMKAQQAVVLVCEHDPAVIQEIADRAYGLKAGRIVFSGS
                     AEALVHTAAFTESYD"
     gene            complement(3975..4433)
                     /locus_tag="VEW91_05095"
     CDS             complement(3975..4433)
                     /locus_tag="VEW91_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYM90994.1"
                     /translation="MSADARLKELKIQLPPIPKPAGTYVHAVRTGNLLFLAGKGPHNP
                     DGSVPKGKVGRDVSAEEANRHARSVGLTLLAVMKDALGSLDRVRRVVKVLGMVNAVPE
                     FGEQPRVINGCSDLFVEVFGERGQHARSAVGMGSLPNGITVEIEAIVEVE"
     gene            4550..5740
                     /locus_tag="VEW91_05100"
     CDS             4550..5740
                     /locus_tag="VEW91_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM90995.1"
                     /translation="MKPTTPRDEVERRIGALQRHLGASALDAALIVQNIDLFYFTGSM
                     QSGALIVPAAGRAVYAVRRVLERARQHSPLEQIIPFDSFRQLPDLVRAAAGAPVRRIG
                     LELDVVPVLTKDRIAAAFEGAEWADVSPAIRAVRSVKSPFELDRIRAAAGLSVVMLEA
                     ATQSLREGMTELELAGRVEAAARRAGHQGQVPMRGWNQAVFFGQLSSGEASALQSFPD
                     IPLAGEGPSPASPIGAGRRRIVAGEPVILDYVAALDGYLCDQTRTLSIGALPEKFARA
                     HDAAVAILRTVQGVIRPGTTPQALYRRALELAKEFGVADHFMGHGQLRARYLGHGVGL
                     ELDEWPILADGWTQPLEVGQVFCVEPKIVFPGEGAVGIEDQFAVTADGSERLTRPEQR
                     LFRV"
     gene            5834..>6859
                     /locus_tag="VEW91_05105"
     CDS             5834..>6859
                     /locus_tag="VEW91_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYM90996.1"
                     /translation="MARARATQAGTEVSEAEIAVHWKEEAYYYPSAKFIGQANLKDPA
                     INRRFSVDRFPECFREYADLLTWDQYWHTTLDTTNAPFWKWFVGGRLNVSSNCVDRHL
                     ARYKNKAALIFVPEPEDEPPTAITYQELYVRVNEVAALLRDFAGLRTGDRVTLHLPMT
                     PELPITMLACARLGIVHSVVFGGFSGEACGIRIADSGSHVLITMDGYYRNGKLLDHKP
                     NADIAIATAQKEGQQVEKVLVWRRLRDRSATTAPKDKGRDYIMNDLLKDYAGSHVSPV
                     SMPADAPLFLMYTSGTTGRPKGCQHSTGGYLAYVAGTSKYIQDIHPTDVYWCLADIGW
                     ITGHSYIV"
BASE COUNT          961 a         2353 c         2445 g         1100 t
ORIGIN      
        1 cggctttgac cgaagcgccc cgcatcgtgc gggcggcgtg ctggatggcg ttgacgatat
       61 gctggtagcc ggtgcaccgg cacaggttgc cctcgatccc ccaccggatc tgctcctcgg
      121 tgggatcggg atggcgctgg agcaggtcga cgacggtcat gatcatcccg ggggtgcaga
      181 agccgcactg caggccgtgt tcctcccaga acccttcctg aatggggtgg agcttgccgt
      241 cttgcgccag gccttcgatc gtctggagcg ctgcgccgtc tgcctggacc gccagcagcg
      301 tgcaagcctt gaccgccttg ccgttcatca gaagcgtgca cgccccgcag ttgctggtat
      361 cgcacccgac atgcgtgccg gtgagcccca acacctcccg cacataatgc accagcagga
      421 gccgcggctc gacctcgtgg gtgtgcgtaa cgccgttgac ggtgactgtg accgcccgct
      481 tcaccgatgg tccccccttc accccatgcg ctgccacacc ggatgccgac aagacctcaa
      541 gatgtctggt gggttctgaa tgctgcggct gcagcgaacg gaccggcgtc gtgtgagtgc
      601 attatagcga gcggtcttca cgcgaagcaa gaggcgtgct agcatgcagc cgggggggat
      661 tcagatcgag cgtgtgtttg ctgcggccgc ggtcctgatt ctgctcgcgg gggcgcacct
      721 gggtccggcc gccgccccgc cgatcgcggc cgggtaacgt ggaggcggtc ctccaggtcc
      781 tcgtcggcgg cgtgttcacc ggggccgaat acgcgctcgc ggccgtcggg atgacgctga
      841 tcttcggcgt cggccgggtg gtcaacctgg cgcagggcag cttcgttgcg ctcggcgcct
      901 acgtcgcgta ccaggtgacg ttgctcggcc tccccgcgcc cctcggtgcc gtgccggcgg
      961 cggcggccgg cctggtgctg ggcgcggcag tcgaccgcat cgtggtgcgc cccacccgcc
     1021 gagaggccct gaccgcggcc acggtcctcc tcgggctcgc gatccttgcc gagcaggggt
     1081 tcgcgctggt ctgggggacg tcgtcccatt cggtaccgct gcgcctgccc acgttcctag
     1141 ttggccgtat cgtggtgggc gtcgagcaga tggccgcggg ggccgcggcg gccgtcgccc
     1201 tcggaaccgt ggccctctac ctgcggacga aaccgggact ggcgcttcgg gcggccgccg
     1261 tcgacgcgga gatcgccgcg ctcggcgggg tcgatgtcga acgcattcaa acggccacct
     1321 tcggcgtggc ctgcgggatg gcagcggccg cgggggcgtt gctcgcgccg ctgctcaccc
     1381 tcacgcctgc gatgggacgc gttcccctgg tgctcctgct ggcgatggtg gccgccggag
     1441 ggccggaccg catttggggc acgctcgtcg caggcttcgg gatcgggctg gcctcaacga
     1501 tcatcggctt ctatctcgac ccggcgtggt cgtatgtgtt cgccctcctg gtgctgatcg
     1561 gggcggtcct ctggcgcgcc gacggggtgc tcgggcggat gcgcccgtga cgccctggcg
     1621 gatcgcctgg tgtctgcttc cggtcgggct cgccctcgcg gcccaggacc gcttcatcct
     1681 cgccatcctg gcgctcgccg ccgtgcaggc gctctacgcg gccagctggg atctcgccgg
     1741 cggcgtgagc gggcagccga ccctggggca cgcgctccct ctgggggccg gcgcctatgc
     1801 cacggcgctc gtctccggat ggggactggc cccgccgccc gtggcgatcg ccagcggggc
     1861 gctcgccggc gggctcatcg gcgccctcca gggaaggctt gtggcgcggc tccacccggt
     1921 ctcggtagcg ctcgtcacgc ttgcgacagc cgagtgcgtc cacgagatga gcgggatggt
     1981 cagggtgcag tggccgggcg ggatcatcgt gggtggcgac agcggcatcc ccgcggtcat
     2041 ctaccccccc ggtgagaccg cggcagccta tctgacggct gcggtgctca ccgcggggct
     2101 ggtcgggctc ttgtggatcg cgcattcggg tcccgggctg gccatgcgcg tcgctcgtac
     2161 cgatccccgg ctggccgccg cctccggtat cgatgtcgcg cgggtccgca tgctggcctt
     2221 gaccatcgcg ggaacgaccg cgggcctcgc cggggggctc ggggcggccc tggttggacg
     2281 cgcatcaccg tcgacgctgg cgctggacgc cagcctgttt gcgatggcca ccgcaggggt
     2341 gggcggccgc ggttcgatca tcgggccggc agcggtcgcg tacgcgacga ccgccgtctt
     2401 ccagtggctg gacgttcccg caacgggccg cctggcgctg tacgccctgc tgctgatcgc
     2461 cgccgggctt gtgccctcgg ccgcaacgcg gctggggggc gaccgccaca cacgcggggg
     2521 cccggaggga gcgcgcgcga agtgatccgg cgcgccggaa tgccggccgc atccgaaccc
     2581 gctctgcttc gggtcgagga tgtggctaag cgcttcggcg gcgcgaccgc gctcgacggc
     2641 ctgacgctct cactgggggc cggcgaatcc ctgggaatca cggggccgaa cgggtgcggg
     2701 aagacgaccc tcattgacgt cgtctccggg ttcatccgcc ctgaccgcgg gcatgtgtgg
     2761 ctcgccgggc gcgacatcac ccggtggccg ccccaccggg tcgtgcgggc cggtctcgcg
     2821 cggacgtttc aacagccgcg gctggcgccc cgcctgaccg tcgagcagat cctcgaggcg
     2881 gcggtgctcc atcggcgcct cggaagctct ggaaggcgcc gggtggtgac ccagatcctg
     2941 cacaccgtgg ggcttgcgga cctgcggcgg cgggaggggt ggacgctgcc gttgggggcg
     3001 atccggcgtg ttcaggtggg atgcgcgctc gccgccggcg ggcggctgct cttgctggac
     3061 gagccctttg cgtcgctcgg cccggaggac gccccggagg tggtctctgt cctccgccac
     3121 ctccggaggg acggcgtctc gatgctgatc gtcgcgcaca gcccggcctt gttgcaggcg
     3181 ctctgcgacc ggatcgccgt cgtcgaggcc ggacgggtgg tccagaccgg cagccccgcc
     3241 gaggtgctgc gtgcttgagg cccgcgacct gctcgtgcgc tacccgcgcg gtccggtggc
     3301 gctcgacggg gtgagcctga tcgcgcaccg gggcgaggta gtcgccctcg tcgggccgaa
     3361 cgggtcgggt aagtcaacct tgctgcgggt gatcgccggg ctcatcccgc cgcagggcgg
     3421 caccatcagc ctggatcgcg ccccgctcac gcgccttgcg ccgcaccaga gggtcgctcg
     3481 cggcatcgcc cacgcgcccg aacaggcccg gatcctgccg acgctctcgg tccgggacaa
     3541 cctcgccatc ggcgcgtggc tgcgcagaga ccggcgagcg gtcgcgcgcg acctcgagcg
     3601 ggtctgcgag tggttcccgg cgctcaagga acggcagcgc ggtccggccg aggccctgtc
     3661 cggcggcgaa cgccagatgc tggcgctcgg cagggtgctg atggccgccc cacgggtgct
     3721 gctgctcgac gaacccctga ccgggctcga cgccgaatcg cgccggcgca tcgtcgcggt
     3781 catcaatatg atgaaagcac agcaggccgt ggtcctcgtg tgcgagcacg atcccgccgt
     3841 gatccaggag atcgccgacc gcgcctacgg cctcaaggcc ggccgtatcg tcttcagcgg
     3901 ctcggcggaa gccctcgtcc acacggccgc gtttaccgag agctatgatt gatccgtggg
     3961 cggggagccc gctctcactc gacttcgacg atcgcctcga tctcgacagt gatgccattg
     4021 gggagggagc ccatgccgac cgccgaacgg gcgtgctgcc ctcgctcgcc gaacacctcg
     4081 acgaacaggt ccgaacagcc gttaatcacc ctgggctgct cgccgaattc cggtacggcg
     4141 ttcaccatcc ccaggacctt gacgacgcgc ctgacccggt cgaggctccc cagcgcgtcc
     4201 ttcatcacgg ccagcagcgt gagcccgacc gagcgggcgt gccggttcgc ctcctcagcg
     4261 gacacgtccc ggccgacctt gcccttcggg accgagccgt cggggttgtg gggacccttg
     4321 cccgccagga agaggaggtt ccccgttcga acagcgtgga cgtaggtacc ggcgggcttc
     4381 gggatggggg gcagttggat cttgagctcc ttcaggcgtg cgtcggccga catgggcgct
     4441 cccttcttgt gcggactggc acgacacgcg gtgctccgac cacattattg attggaggcg
     4501 tccctgggat tatcctcgtg cattgcccgg cgcccgccgt gccgctatcg tgaaacctac
     4561 gactccacga gacgaagttg agcgccggat cggcgcgctg caacgccacc tcggcgccag
     4621 cgcgctggat gccgccctca tcgttcagaa catcgatctc ttctacttca ccggcagcat
     4681 gcagagcggg gcgctcatcg tgccggccgc cggccgggcg gtctacgcgg tccgccgggt
     4741 cctcgagcgc gcccgacagc acagcccgct cgagcagatc atcccgttcg acagcttccg
     4801 gcagctgccc gacctggtcc gggcggcggc cggcgcgccg gtgcggcgga tcgggctcga
     4861 actggacgtg gtccccgtgc tcacaaagga ccgcatcgcg gcggcattcg agggagccga
     4921 gtgggcggat gtctcgcccg cgattcgcgc cgtgaggtcg gtgaagtccc cgtttgagct
     4981 cgacaggatc cgcgcggcgg cgggcctctc cgtggtgatg ctcgaggcgg ccacgcaatc
     5041 gctgcgggag gggatgaccg agctggaact ggccggccgg gtggaagcgg cggcgcggcg
     5101 cgccgggcac cagggccagg tgcccatgcg cggatggaac caggcggtgt tcttcggcca
     5161 gctgtccagt ggggaggcga gcgcgctcca gagctttccg gacatccccc tggccggcga
     5221 gggcccgagc ccggcgagcc ctatcggcgc cggacggcgc cgcatcgtcg cgggggagcc
     5281 cgtgatcctc gactacgtcg cggcgctgga cggctacctg tgcgaccaga cccgcacgct
     5341 gtcgatcgga gcgctgcccg aaaagttcgc ccgggcccac gatgccgccg ttgccatcct
     5401 gagaaccgtc cagggcgtga tccgcccggg cacgaccccc caggccctct accgacgcgc
     5461 gctggagctc gcgaaggagt tcggggtcgc agaccacttc atgggccacg ggcagctccg
     5521 ggcccggtac ctcggccatg gggtgggcct cgaactcgac gagtggccga tcctggccga
     5581 cggatggacg cagccgctgg aagtgggaca ggtcttctgc gtcgagccca agatcgtgtt
     5641 ccccggcgaa ggcgcggtgg ggatcgaaga tcagttcgcg gtgaccgccg acggaagcga
     5701 gcggctcacc cggccggaac agcgcctgtt cagggtctag gggggaggcc ggcgcaggag
     5761 ggatccggcg cagaccagaa acacacgggt aggccgcttg ggatcaccca ctcccacgaa
     5821 ggaggttcga tctatggctc gtgcccgcgc gacgcaggcc ggtacggagg tcagtgaggc
     5881 cgagatcgcc gttcattgga aggaagaggc gtactactac ccttctgcca agttcatcgg
     5941 gcaggcgaat ctcaaagacc cggcgatcaa ccggcgattc agcgtagacc ggtttcccga
     6001 gtgctttcgc gagtacgccg acctcctgac ctgggaccag tactggcaca cgacgctcga
     6061 taccaccaac gcgccgttct ggaaatggtt tgtcggcggc cggctcaacg tgtcgagtaa
     6121 ctgcgtggac cggcacctcg cacggtacaa gaacaaagcc gcgctcatct tcgtccccga
     6181 gcccgaggac gagccgccca cggcgatcac gtaccaagag ctgtacgtcc gggtcaacga
     6241 ggtggcggcg ctgctcaggg acttcgcggg actgaggacc ggggatcgcg tcacgctcca
     6301 tctgccgatg accccggaac tcccgattac gatgctcgcc tgcgcccggt tgggcatcgt
     6361 ccactcggtc gtgttcggcg gcttcagcgg cgaggcgtgc ggcatccgga tcgcggactc
     6421 cgggagtcac gtcctcatca ccatggacgg gtactaccgg aacgggaaac tgctcgacca
     6481 caagcccaac gcggacatcg ccatcgcgac ggcccaaaaa gaaggccagc aggtggagaa
     6541 ggtcctggtg tggaggcggt tacgggacag atcggccacg acggccccga aggacaaggg
     6601 gcgggactat atcatgaacg accttctcaa ggactacgcc gggagccacg tcagtcccgt
     6661 ctcgatgccg gcggacgcgc cgctcttctt gatgtacacg agcggaacga ccggacggcc
     6721 gaaggggtgc cagcacagca cgggcggata cctggcgtat gtggcgggga cgtccaagta
     6781 cattcaggac atccatccaa cggacgttta ctggtgcttg gccgatatcg ggtggatcac
     6841 ggggcattcc tacatcgtc
//
LOCUS       DASQGH010000263         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23860, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000263 DASQGH010000000
VERSION     DASQGH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23860"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VEW91_05110"
     CDS             complement(<1..702)
                     /locus_tag="VEW91_05110"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYM90997.1"
                     /translation="MAADYHMHLERGPWTLAWLERFVETARARGLSEIGFSEHPHRFR
                     EWRRTLYPAELPSWVDALNTERLDSYLGLVEEARRAGLPVRCGIEWDFFPGCEASLER
                     AIRAHPWDYAIGSIHWLPPEERGGLWWDFDNLSQAGAWAGRDVLDAYRRYFRILNEAA
                     RSRLFDFIGHADVIKVCGYRPDDDISELYESAASAFAASGVCLEINTAGWRKPVGEVY
                     PAPPFLQACRRAGVPV"
     gene            complement(729..1481)
                     /locus_tag="VEW91_05115"
     CDS             complement(729..1481)
                     /locus_tag="VEW91_05115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HYM90998.1"
                     /translation="MARRFSGKVALVTGSGRGLGRAIAKALAQEGASLVLNYHKSDAG
                     ARDAAEEAAKFGGRAIVVRADVSRASEVRLLFAEAQRAFGRLDILVNNAGVFSRVPLL
                     EVTEALWDDVLGTNLKGTFLCAQAAARLMTAQQAGVIVNLASGGGLSPRPGYETSAPY
                     AASKAGVIMLTRRLALELAPAIRVNAVAPGIIESRPDWSEASRKRLGAAALLGRVGAP
                     EAIANAVVFLASDDAASITGHTLVVDGGIVLR"
     gene            complement(1491..2189)
                     /locus_tag="VEW91_05120"
     CDS             complement(1491..2189)
                     /locus_tag="VEW91_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYM90999.1"
                     /translation="MRVAVFSDVHGNVRALEAVLADLEARGPFDQVVNGGDLAYGGPR
                     PREAMDLLMPRAYPTVLGNTDVWIAGIEPGGGRVVAWARRQLLPHHEAFLRRLPRAHR
                     FEPPGGPPLVVVHATPTSLSDLLTPDAPAGDVATMFKQARARTLVYGHTHKAYVRDVA
                     EGMVVNTGSVGFPFDGIPKPSFAILQLEGGRWHAEIVRVAYDHEAVAKELAAGTHPDG
                     PTFARRILTASVSG"
     gene            complement(2200..2814)
                     /locus_tag="VEW91_05125"
     CDS             complement(2200..2814)
                     /locus_tag="VEW91_05125"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYM91000.1"
                     /translation="MDVQTLLTQPEALRAHVQAVLAHHEGAPRELPAGIRRAAVLLVL
                     VHARGEAALLLTERTHLVEHHKGQISLPGGVVESGESPLDAALRETGEEIGVRAQDVT
                     VLGALDEEETVVSGFMLTPFVASIPYPYPLRVSSAEVHRVIEVPIRTLLDPQNVRTEL
                     WDREGVPRVIHFYTVGTDVVWGATGRIIAQFLRRVFPPAAHRRG"
     gene            2917..3771
                     /gene="thyX"
                     /locus_tag="VEW91_05130"
     CDS             2917..3771
                     /gene="thyX"
                     /locus_tag="VEW91_05130"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HYM91001.1"
                     /translation="MPDPLADPTTKRGRAVGLVSLPGGREITIYAVRPVAMLLASTPD
                     ADRLAAMAARQCYSAGSVLDLPPPLSPKQTAHLVNRVVSAGHTSCVEHNQFVFGVRGI
                     SRSCSHQLVRHRVGWSYEQQSQRYVDFSTQEFVEIVVPPTILRLPEMLASFLEPVKAA
                     VHAYFRMRDERVHQEDARYLFNNAFETKLMISANARALLHFFELRTCNLAQWEIRWLA
                     HLMRAELQRVAPEIFKFAGPTCMTQKICWEGPRGAKCPRLAATGAALRDRTPALLAAE
                     AERTSPAS"
     gene            3848..4141
                     /locus_tag="VEW91_05135"
     CDS             3848..4141
                     /locus_tag="VEW91_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HYM91002.1"
                     /translation="MTYTITEPCINVKDRSCIEVCPVDCIHPKTDEDAGEVMLYIHPD
                     ECIDCGACESVCPVTAIFAEADVPDQWKGFTEMNADYFKMSKEEFTAKHGRAP"
     gene            complement(4174..>4678)
                     /locus_tag="VEW91_05140"
     CDS             complement(4174..>4678)
                     /locus_tag="VEW91_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HYM91003.1"
                     /translation="EHVRGFDTVTLCLTKGLGCPVGALLAGDRALIKQALRYKHLFGG
                     AMRQAGIIAAAGLYALDHNIDRLAEDHTNAKILARGLAEIPGVRVEIEHVQTNIVFFD
                     VAGLGITAKDAVERLRAQGVRMGATGRTLIRAVTHLDVSQSNVERAAEIAQQVLGKAQ
                     AKTPGAG"
BASE COUNT          721 a         1737 c         1552 g          668 t
ORIGIN      
        1 caccggcacc ccggcgcggc ggcatgcctg gaggaacggc ggagccgggt agacctcgcc
       61 gaccggcttg cgccatcccg ccgtgttgat ctccaggcag acgccggatg cggcaaaggc
      121 ggacgcggcc gattcgtaga gctccgagat atcatcgtcc gggcggtacc cgcacacctt
      181 gatcacgtcc gcgtgcccga tgaagtcgaa gaggcggctc cgcgcggcct cgttcaggat
      241 ccggaagtac cgccggtagg cgtccaacac atcccggccc gcccacgccc ccgcctgcga
      301 gagattgtcg aaatcccacc acagcccgcc gcgctcctct ggcggcagcc agtggatgga
      361 cccgatcgcg tagtcccagg ggtgcgcgcg gatcgcgcgc tcgagcgagg cctcgcaccc
      421 cgggaagaaa tcccactcga tcccgcacct gaccggcaac ccggcgcggc gcgcctcctc
      481 gaccaggccc aggtagctgt ccaggcgctc ggtgttcagc gcgtcgaccc aggacgggag
      541 ctccgccggg tacaacgtcc gccgccactc ccggaaccgg tgcgggtgct cgctgaaccc
      601 gatctcgctc aggccgcgcg cgcgggcggt ctcgacaaac cgctcgagcc acgccagggt
      661 ccacggcccg cgctcgagat gcatgtggta atccgccgcc atcgccgcct ctatcgtccg
      721 cccgcctttc agcgcaggac gatcccgccg tcgacgacca gggtgtgccc ggtgatcgag
      781 gcggcgtcgt ccgacgccag gaacaccacc gcgttggcga tggcttcggg cgcgccgacc
      841 cgcccgagga gtgcggccgc cccgagccgc ttccgcgatg cctcgctcca atccggccgc
      901 gactcgatga tccccggggc gaccgcgttg acccggatgg cgggggcgag ctcgagggcc
      961 aggcgccggg tcagcatgat caccccggct ttgctcgccg cgtagggcgc cgaggtctcg
     1021 taccccgggc gcggcgacag gcccccgccg ctcgccaggt tgacgatgac cccggcctgc
     1081 tgggccgtca tcagccgcgc ggcggcctgc gcgcacagga acgtgccctt gaggttcgtg
     1141 ccgagcacgt cgtcccacag cgcctcggtc acctccagca gcggcacccg gctgaacacc
     1201 ccggcgttgt tgacgaggat gtccaatcgc ccgaacgccc gctgggcctc cgcgaacagg
     1261 aggcgcacct cggacgctcg ggacacgtcc gcgcggacga cgatcgcgcg cccgccgaac
     1321 ttcgccgcct cctccgccgc gtcgcgggcg cctgcgtcac tcttgtggta gttgagcacg
     1381 agggacgcgc cttcctgagc gagcgctttg gcgatcgccc ggccgagccc ccggcccgag
     1441 ccggtaacga gggcgacctt cccggagaac cggcgcgcca ccgacgggcc ctacccggag
     1501 acgctcgcgg tcaggatgcg gcgcgcaaac gtggggccgt ccggatgggt gccggccgcc
     1561 aactccttgg ccaccgcctc gtggtcgtag gcgacgcgga cgatctccgc gtgccaccgg
     1621 cctccctcga gctggaggat cgcaaacgac ggctttggga tgccgtcgaa cggaaacccc
     1681 acgctccccg tattcaccac catcccctcc gcgacgtccc ggacgtaggc cttgtgagtg
     1741 tgcccgtaca ccagcgtgcg ggcgcgggcc tgcttgaaca tcgtcgcgac gtcccccgcc
     1801 ggggcgtccg gggtgagcag atcgctcagg ctggtcgggg tggcgtgcac gacgaccagc
     1861 ggcggccccc cggggggctc gaagcggtgg gcgcggggga gccggcggag gaatgcctcg
     1921 tggtgcggca ggagctgccg gcgggcccag gcgaccacgc ggcccccgcc cggctcgatc
     1981 ccggcgatcc agacgtcggt gttgccgagc accgtcgggt aggcgcgcgg catcagcaga
     2041 tccatcgcct cccggggccg gggcccgccg tacgccagat cccccccgtt gaccacctgg
     2101 tcgaacggcc cccgggcctc caggtccgcc agcacggcct cgagcgcccg cacgttcccg
     2161 tgcacatccg agaacaccgc gacgcgcatc ctctgctcct tacccgcggc ggtgggccgc
     2221 cgggggaaac acccgcctga ggaactgggc gatgatccgg ccggtggccc cccacaccac
     2281 gtcggtcccg accgtgtaga agtggatgac ccgggggacg ccctcgcgat cccacagttc
     2341 ggtccgcacg ttttggggat cgagcaaggt gcggatcggc acctcgatga cccgatggac
     2401 ctcggcggaa ctcacccgca gcgggtaggg atagggaatg ctggcgacaa acggcgtcag
     2461 catgaacccc gagacgacgg tctcctcctc atcgagcgcc cccagcaccg tcacatcctg
     2521 ggcccgcacc ccgatctctt cgcccgtctc gcgcagcgcg gcgtccagcg gcgactcgcc
     2581 gctctcgacg accccgccgg gcaaggagat ctgtcccttg tggtgctcca ccaggtgggt
     2641 gcgctcggtg agcaggagcg cggcctcccc ccgtgcgtgc acgagcacga gcagcacggc
     2701 cgcgcgccgg atgcccgccg gcagctccct cggcgcgccc tcgtggtggg cgagcacggc
     2761 ctgcacgtga gcgcggagtg cctcgggctg ggtcagcagc gtctgcacgt ccacgaacag
     2821 acctcctcaa aacgttgtca tgataccata caggagggtg gacgagcccg accagcgaat
     2881 acgcccccaa cccagaccgc cgacggaggc cccacgatgc ccgaccccct cgcagatccc
     2941 acgacgaagc gcgggcgggc agtcggcctg gtgtcgctcc ccggcgggcg ggagatcacg
     3001 atctacgccg tgcgccccgt cgcgatgctg ctcgccagca cccccgacgc ggatcgcctc
     3061 gcggcgatgg cggcgcggca gtgctacagc gccgggagcg tactcgacct ccccccgccg
     3121 ctctccccca agcagacggc gcacctggtc aaccgggtcg tctccgccgg gcatacgagc
     3181 tgcgtcgagc acaaccagtt cgtcttcggc gtgcgcggca tctcccgcag ctgctcccac
     3241 cagctcgtcc ggcaccgcgt cggctggtcg tacgagcagc agagccagcg ctacgtcgac
     3301 ttctccacgc aggagttcgt cgagatcgtc gtgccgccca cgatcctccg gctcccggag
     3361 atgctggcgt cgttcctgga accggtcaag gccgcggtcc atgcgtactt tcggatgcgg
     3421 gacgagcggg tacaccaaga ggatgcccgg tacctgttca acaacgcgtt cgaaaccaag
     3481 ctgatgatct ccgccaacgc ccgcgcgctc ctccacttct tcgaactgcg gacgtgcaac
     3541 ctcgcccagt gggagatccg gtggctcgcc catctgatgc gcgccgagct ccagcgggtc
     3601 gcgccggaga tcttcaagtt tgccgggccg acgtgcatga cgcagaagat ctgctgggag
     3661 gggccgcgcg gcgccaaatg cccgcggctc gcggccaccg gcgcggcgct ccgggaccgg
     3721 accccggcgc tgctggccgc cgaggcggag aggacatccc cggcctcgta gtgtataggg
     3781 gattaacgta attatataat gtaataggta tactgtttcc ggagccgacg agaggaggca
     3841 gtgaagcgtg acctacacca tcacggagcc gtgcatcaac gtcaaggacc gctcgtgcat
     3901 cgaggtctgc ccggtcgact gcatccaccc caagaccgac gaggacgcgg gcgaggtcat
     3961 gctgtacatc cacccggatg aatgcatcga ctgcggggcg tgcgagtcgg tctgcccggt
     4021 gacggccatc ttcgccgagg ccgatgtgcc ggaccagtgg aagggcttca ccgagatgaa
     4081 cgccgactat ttcaagatga gcaaggagga gttcaccgcc aagcacggcc gggctccgta
     4141 gccggcattc gacctgcccg accccctcgg cggctacccg gcgccggggg tttttgcttg
     4201 ggccttcccg agcacctgct gcgcgatctc cgccgcccgt tccacgttcg attgcgagac
     4261 atcgaggtgg gtcaccgccc ggatcagggt ccggcccgtg gctcccatcc ggacgccctg
     4321 cgcccgcagg cgctcgacgg cgtccttcgc ggtgatgccc aatcccgcga cgtcgaagaa
     4381 cacgatgttc gtctggacgt gttcgatctc gacccggacc cctgggatct ccgcgaggcc
     4441 ccgcgccagg atcttggcgt tcgtgtggtc ctcggcgagc cggtcgatgt tgtggtccag
     4501 cgcgtagagc cctgcggcgg cgatgatccc ggcctgccgc atcgcgccgc cgaagaggtg
     4561 cttgtaccgc agcgcctgtt tgatcagcgc ccgatccccg gccaagagcg cccccaccgg
     4621 acaccccagc cccttggtca ggcacagcgt caccgtgtcg aacccgcgaa cgtgctcg
//
LOCUS       DASQGH010000264         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49295, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000264 DASQGH010000000
VERSION     DASQGH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49295"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VEW91_05145"
     CDS             <1..513
                     /locus_tag="VEW91_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HYM91004.1"
                     /translation="EELEGAQAYFTREGRCVYCDILKQESGARLRVVWESDEFISFSP
                     FAAWCPFECWVVPKQHEASFGTLRDLQIPALADAMHQTLGRLYQCLNNPPYNYIIHTA
                     PYDAQVGHFYHWHIEIIPRLSQLAGFEWGSGFYINTVPPEDAARYLREVAAPTPAASP
                     EPTRSPRPGA"
     gene            639..1517
                     /locus_tag="VEW91_05150"
     CDS             639..1517
                     /locus_tag="VEW91_05150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM91005.1"
                     /translation="MEIVGFVGLGSMGSGMVRRLLAAGRRVVGYNRTRSKARPLLDAG
                     MEWRDTPRAVAEAADVVCTMVRDTEVLHAVTGGPDGILAGLGPGKIYIDMSTVSPTAS
                     KELAARVAERGARMLDAPVVGSTVTLREGKLLFIVGGDKATCDAVTPILLDIGPKVIH
                     VGANGQAALMKIATNLNIGVQMLAFSEGMLLAEKGGIPRQTAADVLLNSAIASPMLRY
                     RGPFVLKDPDEVLFDVNMMQKDMLLALEMGRTLDVPLPTTAVANEFLTATRAMGLEHK
                     DCAAMYEALARLAGLA"
     gene            complement(1645..>2622)
                     /locus_tag="VEW91_05155"
     CDS             complement(1645..>2622)
                     /locus_tag="VEW91_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C nitrite reductase"
                     /protein_id="HYM91006.1"
                     /translation="PPQSGVFTYDISWGDPGTHRYYLADRANNGIDVIDTTQNRFLYR
                     MAQGQFVGFTGKNDTSGPDGILVIADQQIAWVGDGRSRVKVINVRSGLVQATISTGTT
                     PNRADELAYDPVDHLIAVANNADDPPFVTFISTTDRRVVGKIVYTDATDGIEQSVYEP
                     TSGMFYLSVPETKAEPGGRIDRIDPKTMQIVARYPVSNCHPGGLTLGPQQQLLIGCSE
                     IYAGAHAQSIVIDSRDGHVVATITQVGASDQVWYNPGDNHYFLAARGMTANGLKGGPP
                     TPVLGIVDAAGNQWIANLPTGAGAHSVAADPSNNRIFVPVPNGIAVFSR"
BASE COUNT          438 a          866 c          891 g          427 t
ORIGIN      
        1 gaagagctcg agggcgccca ggcgtatttc acccgtgagg ggcggtgcgt ctactgcgac
       61 atcctgaagc aggaatcggg ggcgcggctc cgggtggtgt gggagagcga cgagttcatc
      121 tcgttctccc cgttcgccgc atggtgcccg ttcgagtgct gggtggtgcc caaacagcac
      181 gaggccagct tcggcacgct gcgcgacctt cagatcccgg ccctcgccga cgcgatgcac
      241 cagacgctcg ggcggctgta ccagtgcctc aacaaccccc cgtacaacta catcatccac
      301 accgccccgt acgacgccca ggtaggccac ttctaccact ggcacatcga gatcatcccg
      361 cgcctgtccc agctggccgg gttcgagtgg ggatcggggt tctacatcaa cacggtgccc
      421 ccggaggatg cggcccggta tctgcgcgag gtggcagccc cgaccccggc cgcctccccc
      481 gagccaacga ggtctccccg accaggggct tagccacggc tgaagaatcc ctctaggcga
      541 gccccgcaca acggggctcc gcggctcccg caccggcgag cgacgcgata cgcctcgccc
      601 accccgcccg agatcacgct gtgagaggag cgaacgtcgt ggagatcgtg ggattcgtcg
      661 ggctcggatc gatggggagc ggcatggtca gacggctgct cgcggcggga cgcagggtgg
      721 tcggctacaa ccgaacgcgc tcaaaggccc ggcccctcct cgacgcgggg atggaatggc
      781 gcgacacccc ccgggcggtc gccgaggccg ccgacgtggt ctgtaccatg gtgagggaca
      841 cggaagtgtt acacgcggtc accggcgggc ccgacgggat cctggccgga ttgggaccgg
      901 ggaagatcta tatcgacatg agcaccgtca gcccgaccgc gagcaaggag ctggcggccc
      961 gcgtggcgga gcgcggcgcg cggatgctcg acgcgccggt cgtggggagc acggtgacgc
     1021 tcagagaggg aaaactgttg ttcatcgttg gcggggacaa agcgacgtgc gatgcggtca
     1081 cgccgatcct gctggacatc ggccccaagg tcatccacgt gggcgcgaac ggccaggccg
     1141 ccctgatgaa gatcgcgacc aacctcaaca tcggcgtgca gatgctggcg ttcagcgagg
     1201 ggatgctgct ggccgagaag ggcggcatcc cgcggcagac cgcggcagac gtgctgctga
     1261 acagcgcgat cgcctcgccg atgctccgct accgcgggcc gtttgtgctg aaggacccgg
     1321 acgaggtcct cttcgacgtc aacatgatgc agaaggacat gctgctggcg ctggagatgg
     1381 gacggacgct cgacgtcccc ctgcccacga ccgcggtcgc caatgagttt ctgacggcaa
     1441 cccgggcgat ggggctcgag cacaaggact gcgccgcgat gtacgaggcc ctggcccggc
     1501 tcgcgggcct ggcctgaaac gccaccgggc cggcggctgc gtgccgacgg cccggcgtct
     1561 cgtctcgatc ggctcccgag acggctactt cggcatctcg gccagccacc agctcatgac
     1621 gcccgcctgc ccctccagct tgtcttaccg ggagaagacg gcgatgccgt tcggcaccgg
     1681 gacaaagatc cggttgttgc tggggtccgc cgccaccgaa tgcgcccccg cgcccgtggg
     1741 caggttcgcg atccactgat tccccgcggc gtcgacgatc ccgagcacgg gcgtcggcgg
     1801 tccgcccttc aggccgttcg cggtcatgcc gcgcgccgcg agaaagtagt ggttgtcgcc
     1861 cgggttgtac cagacctggt cggacgcgcc gacctgcgtg atcgtggcca cgacgtgccc
     1921 gtccctcgag tcgatgacaa tcgactgcgc gtgcgcgccg gcgtagatct cgctgcatcc
     1981 gatcagtagt tgctgctggg ggccgagggt caagccgccg ggatgacagt tgctaacggg
     2041 gtacctcgcc acaatctgca ttgtcttggg atcgatccgg tcaatacgtc cgcccggctc
     2101 cgccttggtc tccgggacgc tcaggtaaaa cattccgctc gttggttcat agacggactg
     2161 ttcgatgccg tccgtggcgt cggtgtacac gatcttgccc accacgcggc ggtcggtcgt
     2221 ggagatgaag gtgacgaacg gcgggtcatc cgcattgttg gcgaccgcga tgaggtgatc
     2281 gaccggatca tacgcgagct cgtcggcgcg gttcggcgtg gtgcccgtcg aaatcgtcgc
     2341 ctggacgaga ccggaacgca cgttaatcac cttgacgcgg ctgcgaccgt cgccgaccca
     2401 agcgatctgc tggtccgcga tcaccagaat gccgtcggga cccgaggtgt cgttcttgcc
     2461 ggtaaatccc acaaactgtc cctgcgccat ccggtagaga aagcggttct gcgtggtgtc
     2521 gataacgtcg atgccgttgt tcgcgcggtc ggccagatag taacggtgcg tcccgggatc
     2581 gccccagctg atgtcataag tgaagacgcc gctctgcggc gg
//
LOCUS       DASQGH010000265         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38111, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000265 DASQGH010000000
VERSION     DASQGH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38111"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VEW91_05160"
     CDS             <1..331
                     /locus_tag="VEW91_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HYM91007.1"
                     /translation="RFYILGGAIALDRFLALADPMSSIAEVAARPLLGAQLAGTESLL
                     EMERAIDRASQRMAAQMYLGDPMALGVVIGYLTRKAAEVSNLRVIAHAKLLGIPGDTV
                     RQEIVVV"
     gene            324..641
                     /locus_tag="VEW91_05165"
     CDS             324..641
                     /locus_tag="VEW91_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HYM91008.1"
                     /translation="MYKVAVITDNETATGFRLAGVEVREAATPEEALGLIREYAAAGY
                     GLLVVNEDLLKGTDEARARLQRGRDLPVVVPLPPARAHLESGEAYIARLVKEHIGFAV
                     KLK"
     gene            688..2493
                     /locus_tag="VEW91_05170"
     CDS             688..2493
                     /locus_tag="VEW91_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HYM91009.1"
                     /translation="MAATAGVITRISGPAVIARGLAGARMYDIVRVGKEKLIGEIIRL
                     DGETAFVQVYEDTSGLYVGEPVESTDAPLTLELGPGMLSSIYDGIQRPLDKIREAQGD
                     FISRGVIVNALDRTRRWAFVPKVRQGDRVGPGDILGEVQEYSYAHRVMVPPNTAEGEV
                     AEIRRGELTVTDVVARLRSGTELRMMQTWPVRIPRPVQRKLDPTELFVTGQRIFDVLF
                     PVAMGGTAAIPGPFGSGKTVVQQTLSKWSNADIIVYVGCGERGNEMTDVLTEFPELED
                     PRNGRPLMERTILVANTSNMPVAAREASIYTGVTMAEYFRDMGYRVALMADSTSRWAE
                     ALREISSRLEEMPAEEGYPPYLSSRLSAFYERAGRAMVLGKGERVGAVTVVGAVSPPG
                     GDLSEPVTQSTLRIVGTFWSLDAPLAYRRHFPAINWNRSYSLYEGLLNSWYGKNVAED
                     FTDQRAWVSAILSREAGLQEVVQLVGPDALQDQERLVIETGKMIREYFLQQSAFSEVD
                     ASSSLEKGYWMVRGIRAFYEAALAALDREMPIDEIINLPQNEQIARFKEVPNDRFRAH
                     IDEFLKSLPAAFGEKAKGEVPVPASPGGRGAGPRA"
     gene            2509..>4151
                     /locus_tag="VEW91_05175"
     CDS             2509..>4151
                     /locus_tag="VEW91_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HYM91010.1"
                     /translation="MSLSTKRYTSINYISGPLLFVEGARDLSYGAIVDIHVQDGGMRG
                     GQVIEVSDRNAVIQVFEETRGMDLAKTSLSLREDVARIGVSREMIGRRFNGLGDPIDG
                     LPPIIPEKRLPILGAPINPVAREKPAEFIQTGISTIDVMNTLVRGQKLPIFSGAGLPA
                     NEIAAQIARQAKVLGEAEQFSVVFAAMGITQREAAFFIHEFESTGALARSVVFMNLAD
                     DPTIERLMTPRAALTVAEYLAYEQDMQVLVILTDMTNYCFAADTEVLLADGTVAKIGG
                     LVDTVVARGRDALMSLPPVLSWDGMRSAPARITDVQKLRYHGTMLRVRTASGAEFSLT
                     PDHKVLVDTPDGPVMKPAGTLAPRESIYSTKRLNIPGSEPALLDLLGTLPCYIHLRDR
                     SLERRLAERYGTLRAAAKALGLNYPRWTDSSHKRCVTPAELRRIGDDLRIGPADLSVL
                     IESISVGKRGRLRLGPGFTWAKFLYLLGLVAADGTVYENHAQHAYYVMFSNQELVLLS
                     EFERLVVEIFPGTSVQRHLNQDGVTMLRINSLPLVILAKA"
BASE COUNT          696 a         1338 c         1458 g          659 t
ORIGIN      
        1 gcggttctac atcctggggg gggccatcgc gttggaccgg ttccttgccc tggccgatcc
       61 gatgtcgagc atcgccgagg tcgcggcccg cccgctcctc ggcgcccaac ttgcggggac
      121 ggagagcctg ctcgagatgg agcgcgccat cgatcgggcg tcccagcgga tggccgccca
      181 gatgtacctg ggggatccca tggcgctcgg cgtcgtcatc ggctacctca cccgcaaggc
      241 ggcggaggtg agcaacctgc gggtcatcgc gcacgcgaag ctgctgggga tcccggggga
      301 cacggtgcgg caggagatcg tcgttgtata aggtcgcggt gatcaccgac aacgagaccg
      361 ctaccgggtt tcgtctcgcc ggcgtggaag tccgcgaggc ggcgacgccc gaggaggcgc
      421 tggggttgat ccgcgaatac gccgcggccg gatacgggct gctggtggtc aacgaggatc
      481 tgctgaaggg cacggacgag gcccgggcca ggctgcagcg cggccgcgat ctcccggtcg
      541 tggtgccgct gccgccggcg cgcgcccatc tggagagcgg cgaagcctac atcgccaggc
      601 tggtgaagga gcacatcgga ttcgccgtga agctcaagta gctggagacg gcacgaccgg
      661 agccacaggc tcaagagagg acggggcatg gcagcgaccg caggcgtcat cacacgcatc
      721 tccggcccgg cggtgatcgc gcggggtctg gccggcgcgc ggatgtacga cattgtccgc
      781 gtcggcaagg agaaattgat cggcgagatc atccggctcg acggcgagac ggcgttcgtc
      841 caggtctacg aggatacgtc gggcctgtac gtcggggagc cggtggagtc gaccgacgcc
      901 ccgctgacgc tggagctcgg cccgggcatg ctcagcagca tctacgacgg gattcaacgc
      961 ccgctcgaca agattcggga agcccagggt gacttcatct cccgcggggt catcgtgaac
     1021 gcgctcgacc gcacgcgccg gtgggcgttc gtccccaagg tgcggcaggg ggaccgggtg
     1081 ggtccgggcg acatcctcgg cgaggtccag gagtactcgt acgcgcaccg ggtcatggtg
     1141 ccgccgaata ccgcagaggg cgaggtggcg gagatcagga gaggggagtt gaccgtcacc
     1201 gacgtcgtgg cccgcctccg cagcggcacg gaactgcgca tgatgcagac ctggccggtg
     1261 cggattcccc ggccggtcca gcggaagctc gacccgacgg agctgttcgt gaccggacaa
     1321 cggatcttcg acgtgctctt ccccgtcgcg atgggcggga cggcagcgat ccctgggcca
     1381 tttgggagcg gcaagacggt ggtgcagcag accctctcca agtggtccaa cgccgacatc
     1441 atcgtctacg tgggctgcgg cgagcgcggt aacgagatga cagacgtcct cacggaattc
     1501 cccgaactgg aagatccccg gaacggccgc ccgctgatgg agcgcacgat cctcgtggcc
     1561 aacacttcca acatgcccgt ggcggcgcgc gaggccagca tctacaccgg agttaccatg
     1621 gcggagtact tccgcgacat gggctatcgc gtcgcgttga tggccgactc cacgagccgg
     1681 tgggccgagg cgctccgcga gatctcgtcg cgcctggagg aaatgcccgc ggaggaaggc
     1741 tatcccccgt atctctcaag ccggctctcc gcgttctacg agcgggccgg ccgcgcgatg
     1801 gtgctgggca agggcgagcg ggtcggggct gtcaccgtcg ttggcgcggt gagcccgccc
     1861 ggcggcgatc tctcggagcc ggtcacccag agcacgctca ggatcgtggg cacgttctgg
     1921 tccctcgatg cgccactggc ttatcgccgc cacttcccgg cgatcaactg gaaccgatcc
     1981 tacagcctct acgaggggtt gctgaactcg tggtacggta agaacgtcgc cgaagacttc
     2041 acggaccagc gcgcctgggt gagcgcgatc ctctcgcgcg aggcagggct ccaggaagtc
     2101 gtccagctgg tcggcccgga cgcgctccag gaccaggagc gcctggtcat cgagaccggg
     2161 aagatgatcc gcgagtactt cctccagcag agcgcgttca gcgaggtcga cgcgtcgtcc
     2221 tcgctcgaga aggggtactg gatggtccgg gggatccggg cgttctacga ggccgccctg
     2281 gccgccctcg atcgcgagat gccgatcgac gagatcatca acctgccgca aaacgagcag
     2341 atcgcccggt tcaaggaagt gccgaacgac aggttccggg cccacatcga cgagttcctg
     2401 aagtccctgc cggccgcgtt cggagaaaag gccaagggcg aggtgcccgt gccggcgtcc
     2461 cccggcggac ggggcgccgg gccgcgggcg taggggagga gaccgagtat gtcgctgtcg
     2521 accaagcgct acaccagcat caactacatc tccggcccgc tcctgttcgt ggagggcgcc
     2581 cgggacctct cttacggcgc gatcgtggat atccacgtgc aggacggggg catgcggggc
     2641 gggcaggtca tcgaggtctc cgaccgcaac gccgtgatcc aggtgttcga ggagacccgc
     2701 ggcatggacc tcgccaagac gtcgctcagc ctccgcgagg atgtcgcccg catcggcgtc
     2761 agccgcgaga tgatcggccg gcggttcaac ggcctggggg atccgatcga cggcctgccg
     2821 ccgatcattc cagagaagcg cctcccgatc ctcggcgccc ccatcaaccc tgtggcgcgc
     2881 gagaagccgg cggagttcat ccagaccggc atctcgacga tcgacgtcat gaacacgctg
     2941 gtccgcggcc agaagctccc gatcttctcc ggggccgggc ttcccgccaa cgagatcgcc
     3001 gcgcagatcg cccgccaggc gaaggtgctc ggcgaggccg agcagttctc ggtggtgttc
     3061 gctgcgatgg ggatcaccca gcgcgaggcc gccttcttca tccacgagtt cgagagcacc
     3121 ggcgccctgg cccgcagcgt ggtcttcatg aacctggcgg acgacccgac gatcgagcgg
     3181 ctgatgacgc cgcgggcggc gctgacggtg gccgagtacc tggcgtacga gcaggatatg
     3241 caggtgctcg tcatcctgac tgatatgaca aattactgct tcgcggccga caccgaggtg
     3301 ctgctcgccg atgggaccgt cgccaagatc ggcggtctgg ttgacacggt cgtggcgcgg
     3361 gggagggacg cgctgatgtc tctgccgccg gtgctgtcgt gggacgggat gcgatccgcg
     3421 cccgcacgga tcacggatgt tcagaagctc cggtaccacg gtacgatgct gcgggtgcgc
     3481 accgcctccg gcgccgaatt ctccctgacg cccgaccaca aagtcctcgt tgacactccc
     3541 gacgggccgg tgatgaagcc ggcgggaacg ctggctccgc gggagagcat ctactcaacg
     3601 aagcggctca atatcccggg atccgagccg gcgctccttg atctcttggg aacactgccc
     3661 tgctacattc acctgcggga ccggagcctc gagcgccgtc tagcggaaag gtatgggacg
     3721 ctgcgcgcgg ccgcgaaggc gttgggcctc aattacccca ggtggaccga ttcctcgcac
     3781 aagcggtgcg tgacgccggc cgagttgcgg cggatcgggg acgatctcag gatcggcccg
     3841 gcggacctgt ccgttcttat tgagagcatc tcggtgggca agcggggccg gctgcggctc
     3901 ggcccaggct ttacgtgggc gaagttcctc tacctgctcg gcctcgtggc ggcggacggg
     3961 accgtctacg agaaccacgc gcagcacgcc tactatgtga tgttctccaa tcaggagctg
     4021 gtgctgttgt ctgagttcga gcggttggtt gtcgagatct tccccggcac ttcggtgcag
     4081 cggcacctta accaggacgg cgtgacgatg ctccggatca acagcctgcc gctggtgatc
     4141 ctggccaagg c
//
LOCUS       DASQGH010000266         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_29606, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000266 DASQGH010000000
VERSION     DASQGH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29606"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VEW91_05180"
     CDS             <1..957
                     /locus_tag="VEW91_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM91011.1"
                     /translation="GDATGDGDVAARAGARFVLGRPGDAVMQPVTRIFRVAGVDVEEM
                     FAYLRQHPDEMDVPERWDGAGYTVQDLTAASVVMDAFPMLVAQARAAGELAVPRDRIG
                     VETGPVSGVVTINATRVQDVDGTDPEALSRAEVETQRQMFEVFQFLRRRVPGFQRAWL
                     LDSAYQVGVRETRHVLGDYVLTLDDVLSGRDFSDAIARGAYPVDLHDMRAGVDVLGST
                     VGGGGVTLRKIERAYGIPFRCVVPADVDAVVIAGRAISASHEAAGSVRGQAVCMATGH
                     AAGTIAALSVKEDVVPRALDVTQVQSVLMTQRAILFYRNAHA"
     gene            968..2158
                     /locus_tag="VEW91_05185"
     CDS             968..2158
                     /locus_tag="VEW91_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYM91012.1"
                     /translation="MEKIQYEHRSGVAVTVEAERRVGIGVIGYDGVAKAHLQAILRVA
                     TVFWPLPVRPVLVALAGRSVERLQQAAQRYGAQAVYTDWRRLIEDRRVEILVNAGPND
                     LHADPCIAAASRGLHVLCEKPLARSSAEAAKMWNAATRAGVVHMAGYNYRFIPAVLLA
                     RQMIVEGRLGQVRHFRARFCDDSMVDPSVPYSWRHSRAQAGSGVIGDLAAHAIDLARF
                     LVGDVSAVSATTRVFVDRRPRPQGGEGIVDVEDAIEAVVEFAAGAVGTLDASTFCPGR
                     KNFLTFEVNGTLGSLVFNLERLNELEVFFRGDAVNGFRTVMVTEGHHPYGGRWWPPGH
                     IIGWEQAFVHQLHRFLAVVAGQGQIGPEGATFEDGYQCAVVCDLLARAARDGRRVDVA
                     GFAP"
     gene            2249..3361
                     /locus_tag="VEW91_05190"
     CDS             2249..3361
                     /locus_tag="VEW91_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYM91013.1"
                     /translation="MKITRVRTRQVDVPLPAPFYPAWAPGGMVTQIRLAYVRIDTDAG
                     IYGVAGHEFYGAEEQCVRRVADYLVGEDPLRIEQHAGTLRYLWPYFGTAVWFVELALW
                     DILGKVAGLPVYRLLGSAHDTIPLYASTGQNRTPAQRADDARGLRDEGFRAIKLRVHN
                     ETLAEDIAQVAAVRRAVGDDMAIMVDANQTDVHDAPLPGPHWTYHRALETARALADYR
                     VTWLEEPLPRHAYAELQRLHDASPIPIAGGEVNQGFAELQRLLLDGCYDILQPDVTLC
                     EGLLRIRALAVMAHAAGVLVTPHTWGDPLGMVANLHLAASISNTTFFEYPHDPPAFPA
                     LVYQQTLKEPLVVEDGMVRLPQEPGLGVELQDWVFD"
     gene            3376..>3666
                     /locus_tag="VEW91_05195"
     CDS             3376..>3666
                     /locus_tag="VEW91_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYM91014.1"
                     /translation="MERVRAATLIAPGRYEIREYPLPDPEPGAVLVKMALSGICGTDK
                     HTYQGYTTQYAGSASPKTIPFPIIQGHENVGTVAAIGGDERYLDFEGVPLRVG"
BASE COUNT          558 a         1161 c         1313 g          634 t
ORIGIN      
        1 ggggacgcga cgggggacgg ggacgtcgcc gcgcgcgcag gcgcccgctt cgtcctcggc
       61 cgcccgggcg atgccgtcat gcaaccggtg acgcgtatct ttcgcgtcgc cggcgtcgac
      121 gtcgaagaga tgtttgccta cctccggcag caccccgatg agatggatgt gcccgagcgg
      181 tgggatgggg ccgggtatac cgtccaggat ctcacggccg cgtccgtggt catggacgcg
      241 ttccccatgc tcgtggcaca ggcccgcgcc gccggagaac tggcggtgcc gcgcgatcgg
      301 atcggcgtgg agaccggccc cgtgtcgggt gttgtgacga tcaatgccac gcgcgtccaa
      361 gacgtcgacg ggacggaccc agaggcgtta agccgagcgg aggtcgagac gcagcgacag
      421 atgttcgagg tgttccagtt cctccggcgg cgcgtcccag ggttccagcg cgcatggctg
      481 ctcgactccg cgtatcaagt cggcgttcgg gaaacgcgcc acgtgctcgg cgactatgtg
      541 ctgacgcttg atgacgtgct ctccggcagg gacttttccg acgcgatcgc gcgtggggcc
      601 taccccgttg atcttcacga catgcgcgcc ggggtggatg tcctgggctc cacagtcggc
      661 ggcggcgggg tgacgctgag gaagatcgag cgcgcgtacg gcatcccgtt tcggtgcgtg
      721 gttccggcag acgtcgacgc ggtagtgatc gcaggtcgcg cgatctctgc gtcccacgaa
      781 gccgccggat ctgtgcgcgg acaggcggtg tgtatggcaa ctggtcacgc ggcgggcact
      841 atcgcggcgt taagcgtcaa ggaagacgtg gttccccggg cgctcgatgt cacacaggtc
      901 cagagcgtac tgatgacgca acgagcaatt ctgttctaca gaaatgccca cgcctgagga
      961 ggtggatatg gagaagatcc agtatgagca tcgctcgggc gttgctgtga cggttgaagc
     1021 ggagcgccgg gtcgggatcg gcgtcatcgg ctacgatggc gttgccaagg cccatcttca
     1081 ggcgatcttg cgcgtcgcga cggtcttctg gccgcttccg gtccgtccgg tgctcgtggc
     1141 cctggccggg cggtccgtcg aacgactgca gcaggcggct caacgctacg gggctcaggc
     1201 cgtctacacc gactggcgtc gcctgatcga ggatcgccgg gtcgagatcc tcgtcaacgc
     1261 cggtccgaac gacctgcacg ccgatccttg catcgcggcg gcgtctcggg ggctccacgt
     1321 gctctgcgag aagccgcttg cgcggtcgtc cgccgaggcc gcgaagatgt ggaatgccgc
     1381 tacgcgcgcc ggggtcgtcc acatggctgg ctacaactac cgctttattc cggccgtgct
     1441 gctggccagg cagatgatcg tcgagggacg gctcggccag gttcggcact tccgggcgcg
     1501 gttctgcgac gactccatgg tggacccgag cgtcccctat agttggcggc acagcagggc
     1561 gcaggccggc agcggcgtca tcggggatct cgccgcccac gcgatcgatc tggcccgttt
     1621 cctcgtgggc gacgtcagcg ctgtgagcgc caccacgcgg gtgttcgtgg accggcggcc
     1681 gcgcccgcag ggcggagaag ggatcgtcga cgtcgaagac gcgatcgagg cggtggtcga
     1741 gttcgccgcc ggggcggtgg ggacccttga cgccagcacc ttctgccccg gtcggaagaa
     1801 cttcctgacg ttcgaagtca acggcacgct cggctcgttg gtcttcaacc tggagcgcct
     1861 gaacgagctt gaggtgttct tccggggaga tgcggtgaat gggttccgca cggtcatggt
     1921 gactgagggt catcacccct acggcgggcg ctggtggccc ccgggccaca ttatcgggtg
     1981 ggagcaggcg tttgtgcatc agctgcaccg cttcctcgcc gtggtggcag gccagggaca
     2041 gatcggaccg gaaggcgcaa cgttcgagga tgggtatcag tgcgccgtcg tgtgcgacct
     2101 cctcgcccgg gccgcgcgtg acgggcgccg ggtcgatgtc gcgggattcg ccccctgaca
     2161 ggtctgagcc tcatccatgt gccggttgaa ctgcccccgt gaaatgcgag agccgggcgg
     2221 cccgcggcgt ccctgaccgg agcatttcgt gaagatcacc cgagtgcgca cccgccaggt
     2281 cgatgttccg ctccctgcgc cattctatcc cgcctgggcg ccggggggca tggtgacgca
     2341 gatccggctc gcgtacgtgc ggatcgacac ggatgcgggg atctacggcg tcgccgggca
     2401 cgagttctac ggcgcggagg agcagtgcgt gcggcgcgtt gccgactacc tggtcgggga
     2461 ggatccgctg cggatcgagc agcacgccgg gacgctgagg tacctgtggc cgtattttgg
     2521 aacggccgtg tggttcgtcg agctcgccct ttgggacatc ctcggcaagg ttgccgggct
     2581 gccggtgtac cggttgctgg ggagcgcgca tgacacgatc cccctgtatg cctcgaccgg
     2641 acagaatcgc acacccgcgc agcgggcaga cgacgcgcgc gggctgcgcg acgaagggtt
     2701 tcgggcgatc aagcttcgcg tccacaacga gacgctggcg gaggacatcg cccaggtggc
     2761 tgcggtgcgg cgtgccgtcg gcgacgacat ggcgatcatg gtggacgcca atcagacgga
     2821 cgtgcatgac gccccgttgc cgggtcccca ctggacctac catcgggcgc tcgagaccgc
     2881 ccgggcgctc gccgattacc gcgtcacgtg gctggaggaa ccgctcccgc gccacgccta
     2941 tgcggagttg cagcgcctcc acgacgcctc gcccatcccg atcgcgggcg gtgaggtcaa
     3001 tcagggattt gccgagctgc agcggctgct gctcgacgga tgctacgaca tcctgcagcc
     3061 ggacgtgacc ctgtgcgaag ggctgctgcg cattcgggcc ctcgccgtga tggcgcacgc
     3121 cgcgggcgtg ctggtgacgc cacacacgtg gggtgatccg ctcgggatgg tggcgaacct
     3181 gcatctcgcg gcgtccatct ccaatacgac gtttttcgag tacccccacg acccgccggc
     3241 gttcccggcc ctcgtctacc agcaaacgct caaagagccg ctggtggtgg aggatggcat
     3301 ggtgcgtctg ccgcaggagc ccggcctggg cgtggagtta caggattggg tgttcgactg
     3361 agggaggcct tggacgtgga acgggtgcgc gcagcgacgc tgatcgctcc aggccgctac
     3421 gagatccgcg agtacccctt gccggatccg gagcccggag ccgtcctggt caagatggcg
     3481 ttgtctggta tctgcgggac cgacaagcac acgtaccagg ggtacacgac acagtacgcc
     3541 ggctcggcct cgcccaagac gattccgttt ccgatcattc agggccacga gaacgtcggc
     3601 acggtggcgg cgatcggtgg ggacgaacgc tacctggatt ttgaaggcgt cccgctccgc
     3661 gtcggc
//
LOCUS       DASQGH010000267         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9624, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000267 DASQGH010000000
VERSION     DASQGH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9624"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..555
                     /locus_tag="VEW91_05200"
     CDS             136..555
                     /locus_tag="VEW91_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYM91015.1"
                     /translation="MRGMLIDTGPLVAVVDESDKDHRRCVNVLKRLVDPLVTTWPAVT
                     EATYLLGQTSNPLDSQDALLATIERHVVVVADLGPKDVPRLRALIRKYRDLPMDLADA
                     TLVCVAERDGIRQVFTLDTRDFEVYRIGRRETFTIIP"
     gene            complement(895..>1705)
                     /locus_tag="VEW91_05205"
     CDS             complement(895..>1705)
                     /locus_tag="VEW91_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYM91016.1"
                     /translation="EEARALGATVILHDAGGYQFLDRQIAQMEDLIANRVDAILLVAV
                     SGPGTVPVVEKAYAARIPVININVMTDNPHVAIRIRSDDKSLGRKEGDLMAQALGGKG
                     NVVMLKAAAGASPFELRSAGFKEYLKANTSIRILGEQNSVNTPDQGLKLMEDFMQTFP
                     QIDGVMCAADFLAIGAAQAIRAAGKAGKIKIVTVDLQPDAEKLLRDGLLAGAIVQTAQ
                     IMGRWGVRAAINVLAGRQVPPQLFTPIFIVTKANVDTVDFTLERGPVGWKP"
BASE COUNT          295 a          557 c          529 g          324 t
ORIGIN      
        1 gcggccaaaa cctggcagta cgctctacga taagatggcg gaccttgttg gcatcggtca
       61 cggaggaccg ccggacctgg cttcgcgatc tgaagaaatc cttcgccagc tgttcgctca
      121 gcggcgagga cgacgatgag aggcatgttg atcgataccg gccctctcgt cgccgtggtg
      181 gacgagagcg ataaagatca ccgtcgctgc gtcaatgtac tgaaacggct ggttgatcca
      241 ctggtcacga cctggccggc cgtgactgaa gccacgtacc tgctcgggca gacaagcaac
      301 cccctggact cgcaagatgc tctcttggcc acaatcgagc gccacgtcgt ggtggttgct
      361 gatctgggtc ccaaggatgt tccacggctc cgagccctga tccggaagta ccgggatctc
      421 ccgatggatc tcgctgatgc cacactggtg tgcgtggccg agcgggatgg gattcggcag
      481 gtgttcacgc tcgacacacg ggacttcgaa gtttaccgga tagggcgccg cgagaccttt
      541 acgatcatcc cctaaccgca ggaacttggt gaaccgaaag gaacttgatg tgcgatttcc
      601 cggttcattc tccgcgcagg ttccagaacg aacctttgag tcctacctct gccagaagcg
      661 cctctgtttc ctccaacgta cgcctccgcc attctggctt gaccttgtcc ttgtaccaac
      721 cttccgcgtc tacgtggggc caggacgcga acgcggagag atcagcgtcc ggcgagtgct
      781 ccacccctca cgtcggacga tcgagaaacg ctgaggagaa cctcggcacg ctagagcgcg
      841 aaaccacagc ctggaccgcc catcgctagg gctgcgtggg ggcacgccgg gtcgtcacgg
      901 cttccacccc accgggcccc gctccagggt gaagtcgacc gtgtcgacgt tggccttcgt
      961 cacgatgaag atcggtgtga acagctgcgg cgggacctgg cgcccggcga ggacgttgat
     1021 cgccgcccgc acgccccagc gtcccatgat ctgcgcggtt tggacgatcg cgccggcgag
     1081 gaggccgtcg cgcagcagct tttccgcgtc gggctgaagg tccacggtga cgatcttgat
     1141 cttcccggcc ttgccggcgg cccggatcgc ctgcgcggcg ccgatcgcca ggaaatcggc
     1201 ggcacacatc accccgtcga tctgcgggaa cgtctgcatg aagtcttcca tgagtttgag
     1261 cccctgatcg ggggtgttga cggagttctg ctcgcccagg atcctgatcg acgtgttggc
     1321 cttgaggtac tctttgaatc cggcggagcg cagctcgaac ggcgacgctc ccgcggcggc
     1381 cttcagcatg acgacgttgc cctttccccc cagggcctgg gccatcaaat caccctcctt
     1441 gcgccccagg gacttatcgt ccgagcggat ccggatggcg acgtgcgggt tgtccgtcat
     1501 cacgttgatg ttgatcacgg ggatgcgcgc cgcgtatgcc ttctcgacga ccggcacggt
     1561 ccccggcccc gacaccgcca ccagcaggat cgcgtccacc cggttggcga tgaggtcttc
     1621 catctgggcg atctgccggt cgaggaactg atagcccccc gcgtcgtgca ggatgaccgt
     1681 ggcgcccagt gcccgggcct cctcc
//
LOCUS       DASQGH010000268         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12597, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000268 DASQGH010000000
VERSION     DASQGH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12597"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VEW91_05210"
     rRNA            <1..326
                     /locus_tag="VEW91_05210"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            395..511
                     /gene="rrf"
                     /locus_tag="VEW91_05215"
     rRNA            395..511
                     /gene="rrf"
                     /locus_tag="VEW91_05215"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(633..1055)
                     /locus_tag="VEW91_05220"
     CDS             complement(633..1055)
                     /locus_tag="VEW91_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYM91017.1"
                     /translation="MGPRVSLITLGVADLARARRFYEALGWHSASPADQDVVFFPSGG
                     MVFALWDRAKLAADSGVELGHGYGGVTLAHNLRTPEEVDALISQARAAGATITREPGE
                     TFWGGYSGVFVDPDGYPWEVAHNPHWTLGADGSVTLPD"
     gene            1625..>2698
                     /locus_tag="VEW91_05225"
     CDS             1625..>2698
                     /locus_tag="VEW91_05225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYM91018.1"
                     /translation="MEKFVIEGGVPLSGTMVPAGNKNGALPILASAVLTADEVIVRNV
                     PRIRDVEAMLEILSAIGVHVSWRGPNEVALCAANVHEVEVERDLAELIRASFLLAGPL
                     LARFHRAVMPPPGGDVIGRRRLDPPLDAFRAMGAIVDCGPEIVLTAPRGLRPTDVFMD
                     APSVMATENALMAAALTPGTTVIGNAACEPHVQDLARMLVKMGADIQGIGSNLITVNG
                     AERLHGCEHEVAPDHIEIGSFMALAGVTGGELRIKDTVPGDLRMIRLVFERVGLRSEL
                     DGNDVLVPGGQRLVAQADVGEYKSKIQDGPWPAFPADLTSIAVALATQAEGSILVHEW
                     MFENRLIFTDKLILMGADIVMCDP"
BASE COUNT          446 a          939 c          903 g          410 t
ORIGIN      
        1 ctcagatcga gcgcgggagt aacaaaactg tcggtcccta tctgccgtgg gcgttggaga
       61 gttgagagga tttgtcccta gtacgagagg accgggatga acgcacctct ggtgtatctg
      121 ttgtcctgcc aagggcatag caggctagct acgtgtggat cggataaccg ctgaaagcat
      181 ctaagcggga agccggcctc aagatgaact ctcccatccc ctagagggag taagacccct
      241 ggtagaccac caggttgata ggccggatct ggaaggacgg caacgttcga aggagaccgg
      301 tactaatggg tcgagggctt gacggatttc tttgataccc gctgtggcgc tgtgcagttt
      361 tgaaggaccg gggaggcgtg gtctctcgcc tttttacggt ggctttagcg agggggaaac
      421 acctcttccc attccgaaca gagcagttaa gcccctcagc gccgatggta cttggctcgc
      481 aagggcctgg gagagtagga cgccgccggt ttttcgtgag acacgggccg ccccttgggc
      541 ggcccgccgt cgttcctcgc cgcaacgaac ggcactacct ggaccagcga aggtcggccg
      601 gctccttgcg tgggcgccaa cgcgcccagc cgtcagtcgg gcagcgtcac gctgccgtcg
      661 gcgccgagcg tccaatgcgg gttatgcgct acctcccacg gatatccgtc ggggtccacg
      721 aagacccccg agtagccgcc ccagaacgtc tctccaggct cgcgggtgat ggtggcgccc
      781 gccgcgcgag cctgcgagat cagcgcgtcc acctcctcag gggtgcggag gttgtgcgcc
      841 aacgtcacgc cgccgtaccc gtgaccgagc tccacgccgc tgtccgcggc aagtttcgcg
      901 cggtcccaaa gggcaaagac catcccgccc gaggggaaga acaccacgtc ctgatccgcc
      961 ggagaggcgc tgtgccagcc gagcgcctcg tagaagcgcc gcgcgcgggc gagatccgcc
     1021 acgccgaggg tgatgaggct tactcgcggg cccatccggg tgacgatacc gccgtggcgc
     1081 agcgggcgtg cgagcgctcg acgccatgtg cggcagggcg gacgggggag aggcacacct
     1141 gcgccaccgg ctccggttgc cccagccgcc gctgaggggg ccgccggcgt agcggcgccc
     1201 ggtgcggatg agccgccgga gaatgctcgt cccaatcgac ctcccagtcg acgtcgtggt
     1261 cgtcctcgag cagcaggaac gagcgggtca ggcggagggc accgagcgcg cgccgcgcaa
     1321 gcgttgatgt tcgcggtgac gagaacatgt gttcgcaagc tacgggcggg ctcggacgga
     1381 tcggaggcga acacgtgtac gtgaccggtc ccatgccgga aaacggcatc ccagagccga
     1441 ttctatgtcg ggttgcatgc aacgcgactt gcaagatcga tacggggtgg acctgccccg
     1501 cggccggtgc gtggcggagg cagggcgatc gccggccggc gcgaacctga gggacatggc
     1561 tgttacgctt gccaggcgtc acaccggtgg gcccatcctg ggccggcggc gaggcccaaa
     1621 gctcatggag aagttcgtca tcgaaggcgg cgtcccgctg tccggcacga tggttccggc
     1681 cgggaacaag aacggggccc tgccgatcct cgcctccgcc gtcctcaccg ccgacgaggt
     1741 gatcgtccgc aacgtccccc gcatccgcga cgtcgaggcc atgctcgaga tcctcagcgc
     1801 catcggcgtc cacgtcagct ggcggggacc caacgaggtg gccctctgcg ccgccaacgt
     1861 ccacgaggtc gaggtcgagc gggacctcgc ggagctcatc cgcgcctcct tcctgctcgc
     1921 cgggccgctg ctcgcccgct tccaccgcgc cgtcatgccc ccgcccggcg gcgacgtgat
     1981 cggtcgccgg cgcctggacc cccccctcga cgccttccgg gcgatgggcg cgatcgtcga
     2041 ctgcgggccc gagatcgtcc tcaccgcccc ccgcgggctg cgcccgaccg acgtcttcat
     2101 ggacgcgccc tcggtgatgg ccaccgagaa cgccctcatg gccgccgccc tcaccccggg
     2161 caccaccgtc atcggcaacg ccgcctgcga gccccacgtc caggacctcg cccgcatgct
     2221 cgtgaagatg ggcgcggaca tccagggcat cggctcgaac ctgatcaccg tcaacggcgc
     2281 cgagcgtctc cacggctgcg agcacgaggt cgcccccgac cacatcgaga tcggcagctt
     2341 catggccctc gccggcgtca ccgggggcga gctgcgcatc aaggacacgg tccccggcga
     2401 cctgcgcatg atccgcctcg tgttcgagcg cgtcggcctg cgctccgagc tcgacggcaa
     2461 cgacgtactc gtgcccggcg ggcagcggct cgtcgcccag gccgacgtgg gggagtacaa
     2521 gagcaagatc caggacggcc cctggccggc cttccccgcc gacctgacct ccatcgccgt
     2581 cgccctcgcc acccaggccg aggggtccat cctggtacac gagtggatgt tcgagaaccg
     2641 cctcatcttc accgacaagc tcatcctgat gggggcggac atcgtcatgt gcgacccc
//
LOCUS       DASQGH010000269         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_29618, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000269 DASQGH010000000
VERSION     DASQGH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29618"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1502
                     /locus_tag="VEW91_05230"
     CDS             261..1502
                     /locus_tag="VEW91_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HYM91019.1"
                     /translation="MAAAHNVCGGDAMIVGVVKEASPGERRVALVPGVMPILAKAGWE
                     VVIEAGAGAAAGYPDAAYAEHGARVVPSRSEVFAAADAVIQVMGYGANLQTGRADLAL
                     LRRGQVLIGFLDPLGAPAATREIAATGAVAFAVELMPRLSRAQSMDALSAMSTVVGYK
                     GVLLAAATLPRLFPMLMMAAGTVTPARVLVIGAGVMGLQAIGTARRLGAVVSAYDVRP
                     AVKEEVQSLGARFVELPLEAADAQDARGYARAQDDAFYQRQRELLGRAVGESDVVITT
                     AVVPGARAPVLITDEMVAAMSAGSVIVDLAAERGGNCALTRPGETVVAHGVTILGPVN
                     LASTVPYHASQLYARTAVTFLLHLAKEGQLGRNPEDEITRETLLTIGGEVVQPRVRQA
                     LGLEPLPAGPAGAATRQGGGA"
     gene            1503..1784
                     /locus_tag="VEW91_05235"
     CDS             1503..1784
                     /locus_tag="VEW91_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HYM91020.1"
                     /translation="MPSGLETGLYVFVLATFLGFEVIRRVSPLLHTPLMSLTNAISAI
                     AVVGSIAMAGEQRTALSTILGTLAVTASMTNVVGGFLITDRMLRMFKRK"
     gene            1795..3216
                     /locus_tag="VEW91_05240"
     CDS             1795..3216
                     /locus_tag="VEW91_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HYM91021.1"
                     /translation="MNATLLTLAYIAAAALFVLALKWLSAPATARHGVLAGEIGMLLA
                     VVGTLLGVGIVSYKWIAIACVVGAAIGAPMGIFMPMTAVPQRTALSHAFGALAAALVG
                     AGEYYLQAPRISVFTMTALALEVVLGSLTFTGSLMAFGKLQELLPTRPVTYRGQNIMN
                     LSILTLAVGIAVYLVGHPGQTQLFPMVIALALLFGVLLVIPIGGADMPTVISLLNSYA
                     GLSAAAMGFVLDNKLLIIAGALDGSSGFVLSMIMCRAMNRSFTNVLFGAFGQVRDSAA
                     AKGEQRPTRSATPEEAASILEAARAVIVVPGYGMAVAQAQHKVRELYDVLTRRGTEVK
                     FAIHPVAGRMPGHMNVLLAEADIPYDKLLGIDEINPEFAQADVALVIGANDITNPAAR
                     HDRGSPLYGMPILDVDKARTVMVIKRSMKPGFAGIENELYYLDRTLMLFGDAKSFVGE
                     IVKALAADQAMVGRRQERAAAPA"
     gene            3243..4418
                     /locus_tag="VEW91_05245"
     CDS             3243..4418
                     /locus_tag="VEW91_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYM91022.1"
                     /translation="MAGRGSPGPSLLPVEGLETLCAVLRRRGYRLVGPTVRDGAIVYD
                     EIQGPADLPVGWTDEQDGGTYRLKRRADQARFGYAVGPHSWKKFLFPSATRLWQARRT
                     RDGGFETLENGEPAPRYAFIGVRSCELHAIAVQDRVFLEGAHPDPTYKARRENAFIVA
                     VNCGQAGGTCFCVSMKTGPKATFGYDLALTETLEGGRHAFVVEVGTERGAEVLNEVAH
                     AEAGVEDLKAADRAVARAAAQMGRMMDTTGIKELLYRNYDHPRWDDVAGRCLTCANCT
                     MVCPTCFCSTVEDTSDLTGEHAERWRRWDSCFTMDFSYIHGGPVRSSARSRYRQWMTH
                     KLATWIDQFGTSGCVGCGRCITWCPVGIDITEEVRAIRESERRPAGPPAEEEKDADP"
BASE COUNT          663 a         1523 c         1601 g          719 t
ORIGIN      
        1 acgtcgctac cccctccatc gtattgggcc gagactacgt cccatcccta tacgccccca
       61 tattgcgccc cacacggcac cggatggcat cgggcgaagg ctgtatttgg ggttccgcga
      121 atgggaagag ggaatgagca caacacctcc tcgataccat atatactccg agggggatcc
      181 ctcagcatgc cttgctatcg cgctgccggc ggccgtagaa tgggcaaagg ccacatggca
      241 acagccgtag tatatcgtcc gtggctgcgg cccacaacgt ctgcggcgga gacgcgatga
      301 ttgtcggggt tgtcaaagaa gcgtcgcccg gggagcggcg tgtggcgttg gttcccggcg
      361 tgatgcccat cctcgccaag gccgggtggg aggtggtcat tgaagccgga gccggcgcag
      421 cggccgggta ccccgacgcc gcgtacgccg agcacggcgc gcgcgtcgtc ccgtcccggt
      481 cggaggtctt cgccgcagcc gatgccgtca ttcaggtgat gggctacggc gccaatctcc
      541 aaaccggccg ggcggacctc gcgctgctgc gccgcggcca ggtgctgatc ggcttcctgg
      601 acccgctggg cgcgccggcc gccacccgcg agatcgccgc caccggggcc gtggcgttcg
      661 ccgtcgagct catgccgcgc ctgagccgcg cccagagcat ggatgccctg tccgcgatgt
      721 cgaccgtggt cgggtacaag ggcgtgctgc tggccgccgc gacgctcccc cggctgttcc
      781 cgatgctcat gatggcggcg gggaccgtga cccccgcccg cgtgctcgtc atcggggccg
      841 gggtcatggg gctccaggcg atcggcaccg cgcggaggct tggcgccgtc gtgtccgcct
      901 acgacgtccg gcccgccgtg aaggaggagg tccagtcgtt gggggcccgg ttcgtcgaac
      961 tcccgctgga ggccgcggat gcccaggacg ctcggggcta cgcccgcgcc caggacgacg
     1021 cgttctacca gcggcagcgc gagctgctgg ggcgcgccgt cggcgagagc gatgtcgtca
     1081 tcacgacggc ggtggtgccg ggagcccgcg cgccggtgct catcaccgac gagatggtgg
     1141 ccgccatgtc cgccggttcc gtcatcgtgg atctggccgc ggaacggggc ggcaactgcg
     1201 ccctgacccg tccgggcgag acggtcgtcg cgcatggggt gacgatcctc gggcccgtga
     1261 atctggccag caccgtcccc taccatgcga gccagctcta cgcccgcacg gccgtcacgt
     1321 tcctcctcca tctggccaag gaagggcaac tcggacgcaa cccggaagat gagatcaccc
     1381 gcgagacgct cctgaccatc gggggcgagg tcgtccagcc gagggtccgc caggcgctcg
     1441 ggctggagcc ccttcccgcc ggccccgcgg gggccgcaac acgtcaggga ggaggagcct
     1501 gaatgccatc gggcctcgag actgggctgt acgtgttcgt gctggccacc tttctcggat
     1561 ttgaggtgat ccgccgcgtc tcgccgttgc tgcacacccc gctcatgtcg ctgaccaacg
     1621 cgatctcggc gatcgcggtg gtcggctcga tcgccatggc gggagagcag cggacggcgc
     1681 tcagcaccat cctggggacg ctggcggtga ccgcctcgat gaccaacgtg gtgggaggct
     1741 tcctcatcac cgaccgcatg ctccggatgt tcaagcggaa gtagcgggcc cccggtgaac
     1801 gccaccctcc tcacgctcgc ctacatcgcc gccgcggccc tcttcgtcct ggcgctgaag
     1861 tggctgagcg cgccggcgac ggcccggcac ggcgtgctgg cgggcgagat cggcatgctg
     1921 ctggccgtcg tcgggacgct cctcggcgtc gggatcgtga gctacaagtg gatcgcgatc
     1981 gcgtgcgtcg tcggagccgc catcggcgcg ccgatgggga tcttcatgcc gatgacggcg
     2041 gtgccccagc ggaccgcgct ctcgcacgcg ttcggggcgc tcgccgccgc gctggtgggc
     2101 gccggcgagt actatctcca ggcgccccgg atcagcgtgt tcaccatgac cgcgctggcg
     2161 ctcgaggtcg tgctgggctc cctgaccttc acgggcagcc tgatggcgtt tggcaagctg
     2221 caggagcttc ttcccacccg tccggtgacg tatcgtggcc agaacatcat gaacctctcc
     2281 atcctcaccc tcgccgtggg catcgcggtc tacctggtgg ggcaccccgg gcagacgcag
     2341 ctgttcccga tggtgattgc gctcgcgctc ctgttcggcg tgctgctggt catccccatc
     2401 gggggggcgg acatgcccac ggtcatctcg ttgctcaact cgtacgcggg gctctccgcc
     2461 gcggccatgg gatttgtgct cgacaacaag ctgctcatca tcgccggcgc cctggacggt
     2521 tcgtccggct tcgtcctctc catgatcatg tgccgcgcca tgaaccggtc cttcacgaac
     2581 gtgctcttcg gcgcgttcgg acaggtgcgg gactccgcgg cggcgaaggg cgagcagcgg
     2641 cccacgagga gcgcgacccc ggaggaggcc gcgtcgatcc tcgaggccgc ccgggcggtg
     2701 atcgtcgtgc ccgggtacgg gatggcggtg gcgcaggcgc agcacaaggt gcgggagctg
     2761 tacgacgtgt tgacgcgccg cggcacggag gtgaagttcg cgatccaccc ggtcgcgggg
     2821 cgcatgcccg gccacatgaa cgtgctgctg gccgaggcgg acatcccgta cgacaagctg
     2881 ctcggcatcg acgagatcaa ccccgagttt gcgcaggccg atgtggcgct ggtcatcggc
     2941 gccaacgaca tcaccaaccc ggccgcgcgg catgatcgag ggagccccct ctatggcatg
     3001 ccgatcctgg acgtggataa ggcgcgcacg gtgatggtca tcaagcgcag catgaagccg
     3061 ggcttcgccg ggatcgagaa cgagctgtac tatctcgata ggacgctgat gctgtttggc
     3121 gatgcgaagt cgttcgtcgg cgagatcgtc aaagccctgg ccgccgatca ggcgatggtc
     3181 ggccggcgcc aggagcgggc cgccgccccc gcgtaagggc ggcgtcgggg gtgaagcagc
     3241 cgatggctgg acgcgggtcg ccagggccct cgctactgcc ggtggagggt ctcgagacgc
     3301 tgtgtgcggt tctgcgccgc cgcggctacc ggctcgtcgg gccgaccgtg cgggacggcg
     3361 cgatcgtgta cgacgagatc caggggcccg cggacctgcc cgttggctgg acggacgagc
     3421 aggacggcgg cacctatcgc ctcaagcgcc gcgcggacca ggcccgcttc ggctacgccg
     3481 tcggtcccca ctcctggaag aagttcctct ttccatccgc cacgcgcctg tggcaggccc
     3541 ggcgcacccg ggacggcggg ttcgagacgc tcgagaacgg cgaaccggcg ccgcggtatg
     3601 cgttcatcgg cgtccggtcc tgcgagctcc acgccatcgc cgtccaggac cgggtcttcc
     3661 tcgagggcgc ccaccccgat ccgacctaca aagcccggcg ggagaacgcc ttcatcgtgg
     3721 cggtcaactg cggccaggcc ggggggacct gcttttgcgt ctccatgaag acagggccca
     3781 aagcgacgtt cgggtacgac ctcgcgttga cggagacgct cgaaggcggc cgccacgcct
     3841 tcgtcgtgga ggtcgggacg gagcggggcg ccgaggtcct caatgaggtc gcccacgcgg
     3901 aggcgggggt ggaggatttg aaggcggctg atcgggccgt cgcccgcgcc gcagcccaga
     3961 tggggcggat gatggatacg acgggcatca aggagctgct ctaccgcaac tacgaccacc
     4021 cgcgctggga cgatgtggcc gggcgctgcc tgacctgcgc caactgcacg atggtctgcc
     4081 ccacgtgttt ctgcagcacg gtggaggaca catccgattt gacgggcgag cacgcggagc
     4141 ggtggcgccg gtgggactcg tgcttcacga tggacttctc gtacatccat ggggggccgg
     4201 tgcggtcctc ggccaggtcg cggtatcgcc agtggatgac ccacaagctc gcgacctgga
     4261 tcgatcagtt cgggacgtcg ggctgcgtgg gctgcgggcg atgcatcacc tggtgcccgg
     4321 tggggatcga tatcaccgag gaagtccgcg cgatccggga aagcgagcga cgccctgcgg
     4381 ggccgcccgc ggaggaggag aaggatgcag acccttgagg ccgtcctcgc cggacatccg
     4441 ttcttcagcg gcctctcccg ggacgacctg cagctgatcg ccgggtgcgg cagcaacgtg
     4501 cgcttc
//
LOCUS       DASQGH010000270        11003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_15366, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000270 DASQGH010000000
VERSION     DASQGH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15366"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VEW91_05250"
     CDS             <1..774
                     /locus_tag="VEW91_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HYM91023.1"
                     /translation="RRDRGRWAGRRWGKFFLGCALLGALLPAPAAHATLHPRVVIVFE
                     HAGPSLADLQVIYAMHQPFGLGIFPHMRYSAQIVREAAAHGLVPILHLPMEPRNPADL
                     GPVTGIVWDRMEDAAIAQTVEADLASVPGVVGVSNHAGSLATADRRVMTAVLRVVRSH
                     GLWFEENRTTPAAVASEVAAGFGIPTVLITTYLDDPPVNIDGKVRALIATATRQGWAI
                     AAGHITTGTPHVVARLLPEFRRAGIVFVPITEFLSRRTP"
     gene            771..1787
                     /locus_tag="VEW91_05255"
     CDS             771..1787
                     /locus_tag="VEW91_05255"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYM91024.1"
                     /translation="MSPQPPRARSLDRRTFLRRGGLIASGLALGDLLGLAPEPLAAAP
                     TRVVALIANSWSDTLTLVDGATLEPVGTLPVGREPHKFRQSLDGRTVYSCNTTSNEMI
                     ELDLRTLRPIRHIPMLDPYNVGFPKDGRYLYKLAYRYTFVEVHDARTFRRIKRLETGR
                     QPSHFWFSPDGRYFVNSNQHAHTVTVIDTERMVVAHRLRVDPLPAGIEISADGHYMVV
                     ASGGAGTISVFSTDEWKLVRKLHSGADAHEMVVTRDGRTIFVTNRGENTVSAFEVGPQ
                     KVVAKFPVPGGPDMPMLSPDGSQLWVSGRYNDTATILDTRTLKPIGTVRTGHSPHGIF
                     LAAT"
     gene            1874..2143
                     /locus_tag="VEW91_05260"
     CDS             1874..2143
                     /locus_tag="VEW91_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91025.1"
                     /translation="MGAQRRHLTVGDPRAVDVLDGKIRAKVRDEVAWRRILHGAATAR
                     RRHRQVRSSADLGFYHGLLTGYAVALKALEGKVGQGRSRGTFRGA"
     gene            complement(2156..4873)
                     /locus_tag="VEW91_05265"
     CDS             complement(2156..4873)
                     /locus_tag="VEW91_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/acetate--CoA ligase family protein"
                     /protein_id="HYM91026.1"
                     /translation="MGQEQIAYPAHRATDVVLRDGSMVHIRPIRPEDEQHLLNFLRSL
                     SERSRILRFFAPTSDYTLITLAQLKARVDYRRRFGLIATTGPEDRIVGHAVFAAVDGD
                     DAEVAFAVADEVQGRGLGTILLGHLAEIASAGGIHQFVAFVLPENTQMIQVFRESGFP
                     VEVKHSAGELTVTFPTSLTEEALQRFDRREQVAAVNALRAFLVPRAVAVVGASRHRGT
                     IGGELFHNLLDYGFAGPVYPVNPSAPVVQSVPAYPTVEAVPGPIDLAVIAVPAAEVAR
                     VAEQCARKSVRALVVISAGFAEIGQEGQARQAELVRIYRTSGMRLIGPNCMGLTNTDP
                     AVRLNATFAPTAPPAGRVGFSTQSGALGLAIIDYAASLGIGLSTFVSIGNKPDISGND
                     LINYWETDPNTDVILLYVESFGNPRRFSRIARRVGRTKPIAVVKSGRSRAGSRATSSH
                     TGALLAASDITVDALFRQAGVTRTDTLEELFDVASLLAHQPVPKGRRVGIITNGGGPG
                     ILCADACEAEGLEVPVLTAGAQARLRALLPPQAAVGNPVDMIASAGADHYREAIRIVA
                     DDPGIDALIVIFVPPLVTRPEDVARAIIEATRAGEGAKPILTVFMSARGIPETLRTAD
                     VRIPSFAFPESAAIALAHAARYGEWRARPLGTPPRFEGMERDKAVGVIVAAIGRGDGW
                     LTPEESHALLSCYGLPTVAQSVARTPEEAGAVAAKLGGETALKAISPKIVHKTDLGAV
                     RLRLRGAEATRTAAREMAALLTSKGVPPEAFLVQAMAPEGVEMIVGVVHDPQFGPVIA
                     CGAGGTFVELLKDVSIRLTPLTHEEASEMIRELKTYPLLTGFRGRPRADVGALEDALL
                     RIGTMVEDLHQIAELDLNPIIVHERGAAIVDARIRVAASEPPSLLVRRR"
     gene            complement(4914..7412)
                     /locus_tag="VEW91_05270"
     CDS             complement(4914..7412)
                     /locus_tag="VEW91_05270"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYM91027.1"
                     /translation="MTPQTPRAGPAAAGAHPAPAAPAYVEVALPRVPAAAQRRRLSLA
                     VGRPSRGAGVVDLLLDLEAGRLRVGFDPAELSRDAAHRLATDTAARLLDVDTIARSEL
                     PVERLLPCSTCPLTVECALRRLPGVLGVNVRYASGQVVVDYDPRAASESQIRQRLAAL
                     GVPVRRKDGERITPWWTRHQLALLTGAAFFALALGLGLDHLLGTGRWAVAAYVAAFLA
                     GGWQATRRAIAAVRSGALDVNLLMLASAAGAGTIGYWEEGAILLCLFSLSTTLEAYAM
                     ERTRWAIRGLLALRPEEALVVLDGRECWIPVETLRIGDVITVKPGTRIPIDGTVVSGV
                     SAVDQSALTGESLPVARRGGDPVFAGTVNGNGALEVRVTTLSQETTLARIIALVEEAQ
                     GQKATTQRRIDQLQQGYAIGVLVAAAALATLPPLLLHRPFVAAFYRAMTLLVVASPCA
                     LVVGTPAAVLAAITNGARRGILFKGGVHLERMGRIQAVAFDKTGTLTVGRPRVTEIVP
                     AQGVTPEALLRLAAALEQRSEHPLGAAIVEAAREARTPLPDPESFEAVPGMGIRGTVE
                     GQPLVVGSAALIEEHGILLPEPLEEAAVRLRNLGGTTVFVAGARALGVIGVADPLRPE
                     APEACAALRRLGIRRLIVLTGDHTAVGTAVGAQVDADDVWGDTGPREKAEAIRGLRRE
                     HGEVAMVGDGINDAPALAAATVGIAMGVAGTDVALETADVVLMSGDLRQVAYAVALGR
                     RARQIIRQNVTFALAVIATLVTATLLGHLRLPLAVVGHEGSTVLVVLNGLRLLGTIAP
                     TSAETPPPGSKTHRPGARKAWVRKTPNRAFVRDV"
     gene            complement(7439..8128)
                     /locus_tag="VEW91_05275"
     CDS             complement(7439..8128)
                     /locus_tag="VEW91_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYM91028.1"
                     /translation="MRQAVAREIMSSPVVTVSPDTPFREIVAIMLQHKISGLPVVDET
                     GLLRGIVTEADLLLKEEVPQAQPALIPWHGASLRLERIVDRHRKAEGKTAGELMTENV
                     VTAEEDTSVHRLAHLMLARNVNRIPIVRNGLVVGIVTRADILKLFTRSTQTLLAAVRE
                     VLAHDLWIDPTKLAITCQDGLITIAGEVDRRSDRDLVVRWIKTIDGVVGVDADRLEFR
                     IDDQALGKVVR"
     gene            complement(8308..8976)
                     /locus_tag="VEW91_05280"
     CDS             complement(8308..8976)
                     /locus_tag="VEW91_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM91029.1"
                     /translation="MPSSKPGKIRILLADDHAIVREGVRRILMAEPDMEVVGEAEDGL
                     QAIEQAKLLKPDVAVLDISMPGVNGIEATKQIKAALPETHTLALTMHSDDSYVFQLLK
                     AGASGYVLKRAAATDLVQAIRAARRGEAFLYPSVAKAVVVDYLKRLETGEGRETYDGL
                     TGREKEILTLVAEGATNQEIAQKLYISVKTVQTHRAHIMEKLNLHDRTMLVRYAIRKG
                     LIEP"
     gene            complement(9040..9519)
                     /locus_tag="VEW91_05285"
     CDS             complement(9040..9519)
                     /locus_tag="VEW91_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYM91030.1"
                     /translation="MKTMPRWTAIKVILAPTDLSPGSAAGVIRASELARSLGANLIVM
                     TAIPWPGGTERTEFLDQALDAERMRLAAWFAAYVPSATREGVAVRFLVVAGTPAHAIL
                     EAAETDAVDLIVMATHRQSGLSRLIHGSVAEAVVRAGERPVLTIGTNGAAVRKPAAA"
     gene            complement(9516..10199)
                     /locus_tag="VEW91_05290"
     CDS             complement(9516..10199)
                     /locus_tag="VEW91_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYM91031.1"
                     /translation="MSTPVVTVRPEATLKEVAEKMAAHRVSGLPVVDHLGHVAGIITE
                     SDILTKLGQSAHEEQRLGLLHLFDRLTHATGADRKLHARTAAELMTTPVITARPEASF
                     RELIHLMTSHGINRIPIVEGGRVIGIVTRADLLRTLGRPDAAVTEDVRWRILHDLWVD
                     TTGLDIRTHEGVVTIAGEVGTRSEVDLIKRWIAVTEGVVDVDVRNLRFRLDDRRIEIP
                     SGRVVVPIP"
     gene            complement(10264..10788)
                     /locus_tag="VEW91_05295"
     CDS             complement(10264..10788)
                     /locus_tag="VEW91_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYM91032.1"
                     /translation="MVDSIPRRALLVPTDLSSTSLPAAIYAATLAPRIGAPVVLLHVV
                     SPRQIEEGIAEGRYVDKELEEVRSSVLWWFASLVPPEIRQGLSVTAVAAVGHPEHEIL
                     TTAHAIEARMIVMATHGRTGLPRAVLGSVAESVLRHATCPVLTIPPAARPEAAPSGVA
                     PAREGQEAMTAGSQ"
BASE COUNT         1613 a         3864 c         3827 g         1699 t
ORIGIN      
        1 cggcgggaca gggggcgctg ggccggacga cgctggggta agttcttcct cggctgtgcc
       61 ttgctcggcg cgctcctccc tgcgccggcc gcgcacgcca ccctgcatcc ccgggtcgtc
      121 atcgtcttcg agcacgccgg cccgagcctg gcggatctcc aggtgatcta cgcgatgcac
      181 caacccttcg gtctcgggat cttcccccac atgcgctact cggcacagat cgtgcgtgag
      241 gccgccgcgc acggcctcgt cccgatcctc cacctgccga tggagccgag gaacccggcg
      301 gatcttggtc cggtgacggg catcgtctgg gaccgcatgg aagatgccgc gatcgcccag
      361 acggtcgagg ccgatctggc gagcgtgccc ggcgtcgtgg gcgtgagcaa ccatgccggc
      421 tcactggcca ccgcggaccg ccgtgtgatg accgccgtcc tccgcgtggt gcggtcccac
      481 ggcctctggt tcgaggagaa ccggaccacg cccgccgcgg tcgcgagcga ggtcgcggcg
      541 gggtttggga ttccgacggt tctgatcacc acgtacctcg atgatccgcc ggtcaacatc
      601 gatggcaagg tccgggcgct catcgccacg gccacgcgcc agggatgggc gatcgcggcc
      661 gggcacatca ccacggggac cccccacgtg gtcgcccggc tgctccccga gttccgccgg
      721 gcaggcatcg tgttcgtccc catcacggag ttcctttcga ggaggacgcc gtgagcccgc
      781 agccccctcg ggcccgctcc ctggaccggc gcacgtttct gaggagggga ggcctcatag
      841 ccagcggcct cgcgcttggc gatctcctgg ggctggcccc cgagccgctg gcggctgccc
      901 ccacccgcgt cgtggcgctg attgcgaaca gctggagcga cacgctcacg ctcgtggacg
      961 gcgccaccct cgagccggtc gggacgctcc cggtgggtcg ggagccccac aagttccgcc
     1021 agagcctcga cggccgcacc gtctactcgt gcaacacgac cagcaacgag atgatcgagc
     1081 tcgatcttcg caccctgcgg cccatccggc acatccccat gctcgatccg tataacgtgg
     1141 ggttcccgaa ggacggtcgc tacctgtaca agctggccta tcgttacacg ttcgtcgaag
     1201 tccacgacgc gcggacgttt cgccggatca agcgcctgga gacaggccgt caacccagcc
     1261 atttctggtt ctcgccggac ggacgctact tcgtcaactc caaccagcac gcccacacgg
     1321 tgacggtgat cgacacggag cggatggtcg tggcgcaccg gctccgcgtc gatccgctgc
     1381 cggcggggat cgagatctca gcggacggcc actacatggt cgtggccagc ggcggcgccg
     1441 gaaccatcag cgtgttctcg accgatgagt ggaagctcgt cagaaaactg cacagcggcg
     1501 ccgacgcgca cgagatggtc gtgacccgcg acggccggac gatcttcgtc accaaccggg
     1561 gggagaacac cgtgagcgcc ttcgaggtcg ggccgcagaa ggtggtcgcg aagttccccg
     1621 tgcccggagg ccccgacatg ccgatgctga gccccgatgg gtcgcagctc tgggtctcgg
     1681 gccgctacaa cgacacggcg accatcctgg acacccgcac cctcaagccc atcggcacgg
     1741 tccgcaccgg gcactcgccc cacgggatct tcctggccgc gacctgagcc cggccctcct
     1801 ggaggtgggg tcgcctggcg tctcgtagga ttccacgtga atcagtgctc catgatggga
     1861 tggaggaaga gcgatgggag cgcaacgccg gcatctgacc gtgggggacc caagggcggt
     1921 cgacgttctc gacggcaaga ttcgcgccaa ggtccgggac gaggttgcct ggcggcgcat
     1981 cctgcacggg gccgccaccg cccggcggag gcaccggcag gttcggagca gcgccgacct
     2041 gggattctac cacgggctgc tgaccgggta tgccgtggcg ctcaaggcgc tcgagggcaa
     2101 ggtcgggcag gggcggagta gggggacctt ccgcggggcg tgatcgcgcg ggggctcagc
     2161 gccgccgcac gagcagcgaa gggggctccg atgccgcgac ccggatgcgg gcgtccacga
     2221 tggccgcgcc gcgctcgtgg acgatgatcg ggttcaagtc gagctcggcg atctggtgca
     2281 ggtcctccac catggtaccg atcctcagca gcgcatcttc cagcgcaccg acgtccgccc
     2341 ggggtcggcc tctgaagccc gtcagcagtg gatacgtctt cagctcccgg atcatctcgg
     2401 aggcttcctc gtgcgtgagg ggggtgagcc ggatcgagac gtctttcagg agttccacga
     2461 acgtcccccc cgcgccgcag gcgatgaccg ggccgaactg agggtcgtgg acgaccccca
     2521 cgatcatctc gacgccttcg ggcgccatgg cctgcaccag aaaggcctcc ggcggaacac
     2581 ccttggacgt gaggagcgcg gccatctccc tcgcggcggt ccgtgtcgcc tcggccccgc
     2641 gaaggcggag gcggaccgcg ccgagatcgg tcttgtgcac gatcttgggg ctgatggcct
     2701 tcagggcggt ctcccccccc agcttcgccg ccaccgcccc cgcctcttcc ggcgtgcgcg
     2761 ccaccgactg ggcgaccgtg gggaggccgt agcaggacag gagcgcgtgc gattcctccg
     2821 gcgtcagcca tccgtcgccg cggccaatcg ccgccacgat cacccccacc gccttgtcgc
     2881 gttccattcc ctcgaaccgc gggggggtcc ccagcgggcg cgcccgccac tctccgtacc
     2941 gggcggcgtg ggcgagggcg atcgctgcgg attcggggaa ggcgaacgag gggatccgga
     3001 cgtcggcggt cctgagtgtc tccggaatgc cccgcgccga catgaacacc gtcaggatcg
     3061 gcttggcgcc ctccccggcc cgggtcgcct cgatgatcgc ccgggcgacg tcctccggcc
     3121 gcgtcacgag cggcggcacg aagatgacga tgagggcgtc gatcccgggg tcgtccgcga
     3181 cgatgcggat ggcctctcgg taatggtccg cgcccgcgga ggcgatcatg tcgaccgggt
     3241 tgcccacggc ggcctggggg gggaggaggg cgcgcagccg cgcctgggcg cctgcggtca
     3301 ggaccgggac ctccaatccc tccgcctcgc aggcatcggc gcacaggatg ccgggaccgc
     3361 cgccgtttgt gatgatcccc acccggcggc ccttcgggac gggctggtgg gcgaggagcg
     3421 aagccacatc gaacaactcc tcgagggtgt cggttcgggt caccccggcc tggcggaaca
     3481 gcgcgtcgac cgtgatgtcg gacgcggcga ggagggctcc ggtgtgcgac gaggtggccc
     3541 gggacccggc gcgcgaccgg ccgctcttga cgacggcgat cggcttggtc cggccgaccc
     3601 ggcgggcgat ccgcgagaac cttcgcggat tgccgaacga ctccacgtac agcaggatga
     3661 cgtcggtgtt cgggtcggtc tcccagtaat tgatcaggtc attgcccgag atgtcgggtt
     3721 tgttgccgat cgacacgaag gtggagagcc ctatccccag ggaggcggcg tagtcgatga
     3781 tcgccaaccc gagggcgccg ctctgggtgg agaagcccac gcggcccgcg ggcggggctg
     3841 tgggggcgaa cgtggcattg aggcgcaccg ccggatccgt gttggtgagg cccatgcagt
     3901 tcgggccgat caggcgcatc ccggaggtcc ggtagatccg gacgagctcg gcctggcgcg
     3961 cctgcccttc ctggccgatc tctgcgaacc cggcggagat caccaccagc gcccgaacgc
     4021 tcttgcgcgc gcactgctct gccacccgcg cgacctcggc ggcgggcacg gcgatgacgg
     4081 cgaggtcgat cggcccgggg accgcctcga cggtcgggta cgccggcacg ctctggacga
     4141 ccggggcgct ggggttgacg ggatagacgg gtccggcgaa cccatagtcg agcagattgt
     4201 gaaagagctc gcccccgatc gtcccgcgat ggcgggaggc ccccaccacg gcgacggccc
     4261 gggggacgag gaatgcccgg agcgcgttca cggcggcgac ctgttccctc cggtcgaagc
     4321 gctgaagcgc ctcctccgtg agggatgtgg gaaacgtgac cgtcagctcc cccgcactgt
     4381 gtttgacttc gacggggaag cccgactcac ggaacacctg gatcatctgg gtgttctccg
     4441 gcaggacgaa cgcgacgaac tggtggatgc ccccggccga ggcgatctcg gccaggtgcc
     4501 ccagcaggat cgtgccgagc ccccgcccct ggacctcgtc ggcgacggcg aaggcgacct
     4561 cggcgtcatc gccgtcgaca gcggcaaaca cggcgtggcc gacgatccgg tcctcagggc
     4621 cggtcgtggc gataaggcca aagcggcgcc ggtagtccac ccgggccttc aactgtgcca
     4681 gggtgatcag ggtgtagtcg ctggtcgggg cgaagaaccg caagatccgc gagcgctccg
     4741 acagcgaccg caggaagttc aggagatgct gctcgtcctc cgggcggatc gggcggatgt
     4801 gcaccatgga cccatcccgt aggaccacgt cggtcgcccg atgcgcgggg tatgcgatct
     4861 gctcctgccc catgccgtgc cggtcctccg aaggggtgtt cgctggctgc gtcctacacg
     4921 tctcggacga aggctctgtt cggggtcttc cgcacccagg ccttccgggc gcccgggcga
     4981 tgggtcttcg atcccggggg gggcgtctcg gcggaggtgg gggcgatggt ccccagcagc
     5041 cggagcccgt tgaggacgac gaggaccgtg ctcccttcgt gtccgacgac cgcgagggga
     5101 agccgcaggt gccccaggag cgtcgcggtg acgagggtcg cgatcactgc cagcgcaaac
     5161 gtcacattct ggcggatgat ctgccgcgcc cgcctgccga gggcgaccgc gtaggccacc
     5221 tgccgcagat cgcccgacat caggaccacg tccgcggtct ccagcgccac atccgtcccg
     5281 gcgacgccca tcgcgatccc gaccgtcgcg gccgcgagcg cgggagcgtc gttgatgccg
     5341 tcgcccacca tggcgacctc tccgtgctcc cgccggagcc cccggatggc ctccgctttc
     5401 tcccgggggc ccgtgtctcc ccagacgtcg tccgcgtcga cctgggcccc gaccgccgtg
     5461 cccaccgcgg tgtggtcccc ggtgagcaca atcaggcggc ggatcccgag ccggcggagg
     5521 gccgcgcacg cctcgggggc ttccggccgg agcggatccg cgacgccgat cacgccgagc
     5581 gcgcgcgccc ccgcgacgaa caccgtcgtc cctccgagat tgcgaaggcg cactgccgcc
     5641 tcctcgagcg gttccggcag gaggatgccg tgttcttcga tgagcgctgc gctcccgacc
     5701 acgagcggtt ggccctcgac ggtgcctcga atgcccatcc ccgggacggc ctcgaacgac
     5761 tccgggtccg ggagcggggt tcgggcctcc cgggccgcct ccacgatggc ggcccccagc
     5821 gggtgctcag accgctgctc gagggcggcg gccagccgga ggagcgcttc tggggtgacg
     5881 ccctgggccg gaacgatctc cgtgacgcgg gggcggccca cggtgagcgt ccccgtcttg
     5941 tcgaacgcga ccgcctggat ccgccccatg cgctccaggt gcacgccgcc tttgaagagg
     6001 atgccccgcc gcgcgccgtt ggtgatcgcg gcgaggacgg ccgcgggcgt gccgacgaca
     6061 agcgcgcacg gcgaggccac gacgagcagg gtcatcgccc ggtagaacgc tgcgacgaac
     6121 ggccggtgga ggagcagggg agggagggtg gccagcgccg ccgcggccac gagcaccccg
     6181 atggcgtatc cctgctgcag ctggtcgatc cgccgctgcg tggtcgcctt ttgcccctgc
     6241 gcctcctcga cgagcgcgat gatgcgtgcg agcgtcgtct cttgcgacag cgtggtgacc
     6301 cggacctcga gggcgccgtt gccgttgacg gtcccggcga agaccgggtc gcccccccgc
     6361 cgtgctaccg ggagcgactc cccggtgagg gcggactggt ccaccgcgct caccccggag
     6421 acgaccgtcc cgtcgatcgg gatacgggta ccgggcttca ccgtgatcac atcgccgatc
     6481 cgaagggtct ccacgggaat ccagcattca cggccgtcca gcaccacgag ggcctcctcc
     6541 gggcgcagcg cgagcaggcc ccggatcgcc cagcgggtgc gctccatggc gtaggcctcc
     6601 agcgtggtgc tcagggaaaa caagcacagc aggatcgccc cttcttccca gtagccgatc
     6661 gtcccagccc ccgccgcgga ggccagcatc agcagattga cgtcgagcgc gccgctccga
     6721 accgcggcga tcgccctgcg ggttgcctgc cagcctccgg ccaggaaggc ggcgacgtag
     6781 gcggccaccg cccaccgccc ggtgcccagg agatggtcca ggccgagtcc tagagcgagg
     6841 gcaaaaaagg ccgccccggt cagcagcgcc aactggtgcc tggtccacca gggggtgatc
     6901 ctctccccgt ctttcctccg gacgggaacg ccgagggcgg cgagacgctg ccggatctgc
     6961 gattcgctgg ccgcccgcgg atcgtagtcc acgacgacct ggccggacgc atagcggaca
     7021 ttgacgccca gcacgccggg gaggcggcgg agggcgcatt cgaccgtgag cgggcaggtc
     7081 gaacagggca ggaggcgctc cacgggcagc tcggaccggg cgatcgtgtc aacatcgagc
     7141 agccgcgcgg ccgtatccgt cgccagccgg tgcgcggcat cccgcgagag ctcggccgga
     7201 tcgaaaccga cccgcagccg gcccgcctca aggtcgagga ggaggtcgac cacgcccgcg
     7261 ccccgggagg ggcggccgac ggcgaggctc aggcgccgcc gctgcgccgc cgccgggacg
     7321 cgcggcagcg cgacctcgac gtacgccgga gcagccgggg cggggtgggc cccggctgct
     7381 gccggccctg cgcggggggt ctggggcgtc atccggagcg cggcgcgcgt cgggagcctt
     7441 atctcacgac cttgccgagt gcctggtcgt caatccggaa ctcgagccgg tccgcatcca
     7501 cgccgaccac gccgtcgatc gtcttgatcc agcgaacgac cagatcgcga tcgcttcggc
     7561 ggtcgacctc tccggcgatg gtgatgaggc catcctggca ggtgatcgcc agcttcgtgg
     7621 ggtcgatcca caggtcgtgc gcgagcacct cccgaacggc ggcaaggagc gtttgggtgc
     7681 tgcgcgtgaa cagcttcagg atgtcggccc gagtgacgat gcccacgacg aggccgtttc
     7741 gaacgatcgg gatccggttg acgttccgcg cgagcatgag gtgcgcgaga cgatggacgc
     7801 tcgtgtcttc ctccgccgtg accacgtttt cggtcatcaa ctccccggcc gtctttccct
     7861 cggccttccg atgccggtcg acgatccgct cgagccgcag cgatgctccg tgccacggga
     7921 tcagcgccgg ctgggcctgc ggtacctctt ctttgaggag gaggtcggcc tcggtcacga
     7981 tgccgcgcag caggcccgtc tcgtccacca ccggcaggcc gctgatcttg tgctgcagca
     8041 tgatcgcgac gatctcccgg aagggtgtat cggggcttac cgtcaccacc gggctgctca
     8101 tgatctctcg tgccaccgcc tgtcgcatct caccctcctc ccccgtcttg cggcgctcct
     8161 cgagtccagt atggcaacca cccggccctc accatatcgg tcctgcaaac gaatgcctat
     8221 ccggcgaggg cctgattcgg cgtggtgcag gggactatta cctgctgggg cccgcccggg
     8281 gcggggacgc gtgcccgggt ggacgtgtca gggttcgatc aggcccttcc gaatggcgta
     8341 tctgacgagc atggtccggt cgtgcaggtt gagcttctcc atgatgtgcg cccggtgggt
     8401 ctggacggtc ttcacgctga tgtacagttt ctgggcgatt tcctggttcg tggcaccctc
     8461 ggccaccagg gtgaggatct ccttctcccg tccggtgagg ccatcgtagg tctcgcgccc
     8521 ctcgcccgtc tccagccgct tcaggtagtc gaccaccacg gccttggcca ccgacggata
     8581 gagaaacgcc tccccccgtc gtgcggcccg gatcgcctgg acaaggtcgg tggcggccgc
     8641 ccgcttcagg acatacccgg aggcgccggc cttgagaagc tggaacacgt acgagtcgtc
     8701 ggagtgcatc gtcagcgcaa gggtgtgggt ttctgggagc gcagccttga tctgtttcgt
     8761 ggcctcgatc ccgttgaccc ccggcatgct gatatccaac acggcgacgt ccggtttcag
     8821 tagtttcgcc tgctcgatcg cctggaggcc gtcctccgcc tcccccacga cttccatgtc
     8881 cggctcggcc atgaggatcc gccggacgcc ctcgcggacg atcgcgtggt cgtcggcgag
     8941 cagaatgcgg atcttgccgg gcttgctcga aggcataccg ttgaccgtac catttctttg
     9001 ggatctggcc acgaaagtcc tccggagaat cgccgccggt caggccgccg caggtttccg
     9061 caccgctgcc ccgttggtcc cgatcgtcag cacggggcgc tcgcctgcgc ggaccaccgc
     9121 ttcggccacg ctgccgtgga tgaggcggct caggccggac tgtcgatgcg tcgccatcac
     9181 gatgaggtcg accgcgtcgg tctcggccgc ctcgaggatg gcgtgggcgg gggtcccagc
     9241 gacgacgagg aacctcaccg ccaccccctc ccgcgtcgcc gaagggacgt aggccgcgaa
     9301 ccacgcggcg agccgcatgc gctcggcgtc cagcgcctgg tccaggaact ccgtgcgctc
     9361 ggtgcccccg ggccaaggga tcgccgtcat cacgatcaga ttcgccccca ggcttcgcgc
     9421 cagctccgac gcccggatga cgcccgcggc agagccgggg gagagatcgg tcggggccag
     9481 aatgaccttg atcgccgtcc agcgcggcat cgtcttcatg gaatcggaac caccacgcgc
     9541 cccgagggga tctcaatgcg gcggtcgtcc agccggaagc gcaggtttcg cacgtcgacg
     9601 tcgacgacgc cttccgtgac cgcgatccag cgtttgatca ggtccacctc ggaccgtgtc
     9661 ccgacctcgc cggcgatcgt gacgacgccc tcatgggtgc ggatgtccag cccggtggtg
     9721 tcgacccaca ggtcgtggag gatccgccac cgcacgtcct ccgttaccgc ggcgtccggg
     9781 cggccgagcg tccgaaggag gtcggcccgc gtcacgatgc cgatgacccg tccgccctcg
     9841 acaatcggga tgcggttgat gccgtgcgag gtcatcaggt ggatcagttc ccggaacgac
     9901 gcctccggcc tggcggtgat gacaggggtc gtcatcagtt ccgcggccgt ccgggcgtga
     9961 agtttgcggt ccgcccccgt cgcatgggtg agccggtcga agaggtgcaa caggccgagc
    10021 ctctgctctt cgtgcgcgct ctgccccagt ttggtgagga tgtcagactc cgtgatgatc
    10081 cccgcaacgt ggccgaggtg gtccaccacg ggcagcccgc tcacccggtg ggccgccatc
    10141 ttctccgcca cctctttcag ggtcgcctcg gggcggaccg tgacgactgg tgtgctcatc
    10201 aactcgcgtg cgtgcattga agacccccca tctgccggtt ccgcgccggc cgcgtgtagt
    10261 gtgtcactgg ctgcccgcgg tcatcgcctc ctgtccctca cgagcgggag ccacaccaga
    10321 tggtgcggcc tccggccgtg ccgcgggagg gatcgtgagg accgggcacg tcgcgtgccg
    10381 caggacgctc tccgctacgc tgccgaggac ggcccgcggc agaccggtgc gaccatgcgt
    10441 ggccatcacg atcattcggg cctcgatggc atgcgccgtg gtcaggattt catgttccgg
    10501 atggccgacg gcagccaccg ccgtcacgct gaggccctgg cggatctccg gagggaccag
    10561 cgacgcgaac caccagagca cgctgctccg gacctcctcc agttctttgt cgacgtacct
    10621 gccttcggcg atgccttcct cgatctgtcg cggagacacg acgtggagaa gcacgaccgg
    10681 cgcgccgatc cgcggcgcga gcgtcgccgc gtagatcgcc gccgggagcg atgtgctcga
    10741 cagatcggtg ggcaccagga gcgctctacg agggatcgag tccaccaccc cacctccttc
    10801 tgcttccatg cccacgatag ttccgcgagg aaggtgctgc catcggatgg gcgcttgatt
    10861 tgatctcggg ctcctacccg atgagatgat gtgcgctcac gcgggttggc gcttgggcgt
    10921 ggcgtcgggg cccgtggggg cctcctgggg gggcacgtac cgaagcagcg ccccgaggcc
    10981 atccgggagc gggggctgtg tgg
//
LOCUS       DASQGH010000271         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35201, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000271 DASQGH010000000
VERSION     DASQGH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35201"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..702)
                     /locus_tag="VEW91_05300"
     CDS             complement(361..702)
                     /locus_tag="VEW91_05300"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HYM91033.1"
                     /translation="MPEHTSPPPDGSADDASKQAASPEAVPLAALSASDLLTWVLSLL
                     AAKAWEGMGLVPSPLTSKAEKNMDEARLAIDAYAAVFEVVRARIEEHPRREMENLLTT
                     LRLNFVEKSAG"
     gene            complement(705..1940)
                     /locus_tag="VEW91_05305"
     CDS             complement(705..1940)
                     /locus_tag="VEW91_05305"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HYM91034.1"
                     /translation="MPSARVREHRLRARLTALGAVGRTAEGGVTRLSYRAEHTEAARL
                     TAGWMREAGAEVGVDRWGNLFGVVPGTDRSLPPVAAGSHLDTVPNGGIFDGALGVVAA
                     IEAAAALREAGVAFRHPLLLLAFAEEEGASFRLGCLGSRGVAGRVSDLGSLVDGGGRR
                     AADAIREFDPGVPRMEMPVPMAAYLELHIEQGPILERQRAKLAAVDAIVGIARMAFVF
                     RGEPNHAGTTPMDARRDALWGAADLVSKVREIAQGAEGRAVGTVGQCTISPGAANVIP
                     GRAEVTVEVRSADGRLLDGLCAEITSAAFACAARYDLEVEHRPWWTEPPVPLDPRVRE
                     EVVGAARDLGWPLVTMSSWAGHDAKILAGVAPTGMIFVPSIKGISHSPLEETAWEDAA
                     RGAQVLCRALERLEAWNGG"
     gene            complement(1957..>2470)
                     /locus_tag="VEW91_05310"
     CDS             complement(1957..>2470)
                     /locus_tag="VEW91_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91035.1"
                     /translation="LDDVLMRLTDAMLSFPQLILALAVAAVLGPGLAKAMIAIAVVLT
                     PVFMRLMRAQVLTEREREYVDAARAMGAADRRIIWRHLLPNSLTPIVVQGSLNVAGAI
                     ITEATLSFLGLGTQPPTPSWGSMLNAAQQYLTQAPWLSFWPGVAIAVTVFAVNVAGDG
                     LREIWDPRTR"
BASE COUNT          386 a          911 c          828 g          345 t
ORIGIN      
        1 ggttaccggg gcgggcacgg atggcggggg tcaaccatag aacctggggt tagaccggac
       61 ggctggtgaa cggatgttcg gtactattta tcgggggaaa ggaaaaatac gagaggcgct
      121 ccccaaccgg cgtctcaccg tcttcagctc tcgccgccgg tgcctaccgc ccgccagccg
      181 ttctccgggg ttacccccga agtacactta accagccctc ggcgtccacg tgcgacagcg
      241 tttgagtcgg ctaccttgcc agtcggtttg cgcctccgca agtatttgta gcacaccgac
      301 cccatgggca caatccggaa aatgagttcg agggatatgt ggattgcggg gataaccggg
      361 ctaccccgcg ctcttttcga cgaagttgag ccgcagcgtg gtcagaaggt tttccatctc
      421 ccgacgcggg tgttcctcga ttcgcgcgcg caccacttca aagaccgccg catacgcatc
      481 gatcgcgagc cgtgcctcgt ccatgttctt ctcggccttg ctcgtcagcg ggctgggcac
      541 caggcccatg ccctcccacg ccttggccgc cagcaagctc aggacccacg tgagcaggtc
      601 ggacgccgac agcgcggcga gtggcacggc ctccggcgac gcggcctgct ttgaagcgtc
      661 atcggcagat ccgtccggcg ggggactcgt gtgctccggc atcgctaccc tccgttccac
      721 gcctcgagcc gctcgagcgc gcggcacagc acctgtgcgc cccgggcggc atcctcccac
      781 gcggtctcct cgagcgggga atggctgatc cccttgatgc tgggcacgaa gatcatcccg
      841 gtcggcgcaa caccggccag gatcttcgcg tcgtgcccgg cccagctcga catcgtcacc
      901 agcggccacc cgagatcgcg cgccgccccg accacctcct cgcggacgcg gggatcgagc
      961 gggaccggcg gctcggtcca ccagggacgg tgctcgacct cgaggtcgta gcgcgcggcg
     1021 caggcgaacg cggcgctcgt gatctcggcg cacaggccat ccagcaaccg gccgtcggcg
     1081 ctccgcacct ccacggtgac ctcggcccgg cccgggatca cgttggccgc cccgggggaa
     1141 atagtgcact ggccgaccgt cccgaccgcc cggccttcgg ccccctgcgc gatctcgcgc
     1201 accttggaga cgaggtcggc ggcgccccac agcgcgtccc ggcgggcgtc catcggggtg
     1261 gtgccggcgt ggttgggctc gccgcgaaag acaaacgcca tccgcgcgat gcccacgatc
     1321 gcgtccacgg ccgcaagctt cgcccgctgc cgctcgagga tcgggccctg ctcgatatgg
     1381 agctccaggt acgccgccat cgggaccggc atctccatcc gcgggacgcc ggggtcgaac
     1441 tcgcggatcg catcggcggc gcgccggccg cctccatcga ccagcgatcc caggtcggac
     1501 acgcgccccg ccaccccccg ggaccccaga caccccaggc ggaacgacgc gccctcctcc
     1561 tcggcgaagg cgagcaggag caaggggtgg cggaaggcca ccccggcctc gcgcagcgcg
     1621 gccgcggcct cgatcgcagc aacgacgccg agcgcgccgt cgaagatccc gccgttcggg
     1681 acggtgtcca ggtgggaccc ggcggcgacc ggcggaagcg agcgatcggt ccccgggacg
     1741 accccaaaga gattgcccca gcggtccacc ccgacctcgg ctccggcctc gcgcatccac
     1801 cccgcggtga ggcgcgcggc ctcggtgtgc tccgcgcggt aggagagccg cgtcacgccc
     1861 ccctccgcgg tgcggccgac cgcccccaac gcggtcaggc gcgcgcggag cctgtgctct
     1921 cgaaccctgg ccgagggcat gagcggcccc ccccgatcat cgcgtgcgcg gatcccagat
     1981 ctccctgagc ccgtccccgg cgacgttgac ggcaaacacg gtgacggcga tcgccacgcc
     2041 gggccagaac gacagccagg gcgcctgggt caggtactgc tgggccgcat tgagcatcga
     2101 cccccacgac ggggtggggg gctgcgtgcc gagaccgagg aagctcaggg tcgcctcggt
     2161 gatgattgcc ccggcgacgt tgagcgagcc ctgcacgacg atcggggtga ggctgttggg
     2221 tagcaggtgg cgccagatga tccgccggtc cgccgccccc atcgccctcg cggcatcgac
     2281 gtactcccgc tcccgctcgg tcagcacctg cgcgcgcatg agccgcatga ataccggcgt
     2341 gagaacgacc gcgatcgcga tcatcgcttt ggcgagcccc ggtccgagca cggcggcgac
     2401 ggcgagcgcc aggatgagct ggggaaacga gagcatggca tcggtcagcc gcatgaggac
     2461 atcgtccagc
//
LOCUS       DASQGH010000272         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_26680, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000272 DASQGH010000000
VERSION     DASQGH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26680"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1271
                     /locus_tag="VEW91_05315"
     CDS             48..1271
                     /locus_tag="VEW91_05315"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYM91036.1"
                     /translation="MGYLHHLECTRCSRQYPPPELHNLCRHCGRVLFARYDLERVRAE
                     VPRDALAGRPPTMWRYGEVMPVLDPKHIVTLGEGMTPLLPLEAIGRKLGLRHVFAKEE
                     GLNPTGSFKARGLSAAVSKARELGVRTASMPSAGNAGSAAAAYCARAGMELYLVMPAD
                     VPHVNQAECTMYGAHVYLIHGLINDAGRSLREQRQAKGWFDMSTLKEPYRVEGKKTMG
                     YELAEQFTWSLPDVIIYPTGGGTGIIGMWKAFEEMEALGWIGPHRPRMVIVQAAGCAP
                     IVRAHRNGQEQAEPWQNAQTIAAGLRVPEAIGDYLILQIVRASGGASYAVTDEEILAD
                     MRELAQAEGLLACPEGAATYGALKAMVREGQVAPDERVVLFNTGAGLKYVDLTKPALT
                     AFDPAHPPAALGGRA"
     gene            1335..1490
                     /locus_tag="VEW91_05320"
     CDS             1335..1490
                     /locus_tag="VEW91_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91037.1"
                     /translation="MEGFKWHEIAPETPEGMPGVRIHWVRPDEPHAIKHTGPAPKKFI
                     CCVPAKP"
     gene            1577..>1938
                     /locus_tag="VEW91_05325"
     CDS             1577..>1938
                     /locus_tag="VEW91_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91038.1"
                     /translation="MLRGVLDDAAGRWWLELVEALASSTPSPTRVAETYARLFTLLSG
                     EAELADEPRVGDAWQHHLVGRLLEDENPFSLKAQRAGRSAIGPALLAQARADLTALQR
                     CYRPGAAAIARGVARLTG"
BASE COUNT          310 a          653 c          709 g          266 t
ORIGIN      
        1 tcaagaagtc cgcgctccgg cgcgggcttt ttgatttgga gggacccatg ggatacctcc
       61 accacctgga gtgcacccgg tgctcccggc agtacccccc cccagagctc cacaacctct
      121 gccggcactg cgggagggtg ctgttcgccc ggtacgacct ggaacgggta cgggccgagg
      181 tcccccggga tgcgctggcg ggccgccccc ccacgatgtg gcggtacggc gaggtcatgc
      241 ccgtcctgga ccccaagcac atcgtgaccc tgggagaggg gatgaccccg ctgttgcccc
      301 tggaggcgat tggacggaag ctgggcctcc ggcacgtgtt tgcaaaagag gaggggttga
      361 atccgaccgg gtcgttcaag gcgcgcggat tgtcggcggc cgtgagcaag gccagggaac
      421 tcggggtccg aacggcgtcg atgccctcgg ccggcaacgc cgggtccgcc gccgccgcct
      481 actgcgcccg agcggggatg gagctctacc tcgtcatgcc cgccgacgtg ccgcacgtga
      541 accaggccga atgcacgatg tacggcgcgc acgtctacct gatccacggc ctgatcaacg
      601 atgcgggtcg gagcctgcgc gagcagcgcc aggccaaggg atggttcgat atgtcgaccc
      661 tcaaggagcc ctaccgcgtc gagggcaaga aaacgatggg gtacgagctc gccgagcaat
      721 tcacctggag cctgcccgac gtgatcattt acccgaccgg cggcgggacc ggcatcatag
      781 gcatgtggaa ggccttcgag gagatggagg ccctgggctg gatcgggccc caccgtcccc
      841 gaatggtgat cgtgcaggcc gccggctgcg cgccgatcgt gcgcgcgcac cggaatggcc
      901 aggagcaggc agagccgtgg cagaacgcgc agacgatcgc cgcaggcctg cgcgtgcccg
      961 aggcgatcgg ggactacctg attttgcaga tcgtccgggc aagcgggggg gcctcctacg
     1021 ccgtgaccga tgaggagatt ctggccgata tgcgcgagct ggcccaggcg gaggggctgc
     1081 ttgcctgccc ggaaggcgcg gccacgtacg gggccctcaa ggcgatggtc cgggaaggtc
     1141 aggtggcgcc cgacgagcgg gtggtgctgt tcaacaccgg cgccggcctg aagtacgtgg
     1201 acctcactaa gcccgcgcta accgcgttcg atccggcgca cccgccggcg gcgctgggcg
     1261 gccgggcatg acaggaccgc ggagggcggc gccgaaccct tatgcgcttg ccgcgggctc
     1321 ggagggacgg tccgatggaa gggttcaagt ggcacgaaat tgccccggag accccggagg
     1381 gaatgcccgg ggtgcggatc cactgggtcc ggccggacga gccgcacgcg atcaagcaca
     1441 cggggccggc tccgaagaag ttcatctgct gcgtcccggc gaagccataa gcgacccacg
     1501 acgcgcgcgc caggaccgcc ccccccaccc gaccgcgctg tgggaggaga ttcggggagc
     1561 cgcggcttcg ctgtgcgtgt tgcggggcgt gctcgacgac gcggcgggac gctggtggct
     1621 cgagctggtg gaggcgctgg cctcctccac gccatcaccc acccgcgtcg cggaaaccta
     1681 cgcgcggctg tttaccctgc tctcgggcga ggcggaactc gccgatgagc cccgcgtggg
     1741 ggacgcctgg cagcatcacc tggtgggccg gctgctcgag gacgagaacc ccttcagcct
     1801 gaaagcgcag cgggcgggcc ggtcggcgat cggcccggcg ctcctcgcgc aggcccgggc
     1861 agacctcacg gcactccagc gctgctaccg tccgggcgcg gccgcgatcg cccgcggggt
     1921 cgcgcgcctc accgggat
//
LOCUS       DASQGH010000273        11207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52082, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000273 DASQGH010000000
VERSION     DASQGH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52082"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /gene="miaB"
                     /locus_tag="VEW91_05330"
     CDS             <1..1332
                     /gene="miaB"
                     /locus_tag="VEW91_05330"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYM91039.1"
                     /translation="GNSQRFHIITQGCQMNVRDSQAMAGLLAGMGYQPTEDPGEADII
                     LLNTCTVREGADDRAYGRLGELHALKRRRPGLILGIAGCLVQKDRERVRERAPYLDLV
                     FGVHNIHRLPELLRQVRDGCLPVYEVWDRSDRDRPLPVPPALRGDPIRGFVNIIHGCN
                     KFCTFCIVPYVRGRERSAPPEAVAAEVEGLAAQGYCEVTLLGQNVDSYGHDLTPRRDL
                     ADLLRLVHDVDGIERIRFTTSHPRDMTLKLIRTVAELPKVCEHIHLPVQAGDDRLLRR
                     MHRAYTTAQYRATIDAIRAAMPQASVTTDVIVGFPGETDEEFEGTVRLVNEVQFDAVN
                     TAIYSPREGTPAAGYPDQVPEATKRRRLHALNALVERIAAEVNRALVGTRQEVLVEGP
                     GTRGGMVGRTRTNKVVILDGDAGWIGRTVPVEIASAGSWVLRGRAGSQVLR"
     gene            complement(1317..3050)
                     /gene="mutL"
                     /locus_tag="VEW91_05335"
     CDS             complement(1317..3050)
                     /gene="mutL"
                     /locus_tag="VEW91_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HYM91040.1"
                     /translation="MSSDRGIRILPPSAAERIAAGEVVERPASVVKELIENSLDAGAR
                     RITVEVRGAGRRLMRVTDDGEGIPAEDLPLAFQRFATSKIRTAEDLQGIHTYGFRGEA
                     LPSIAAISRVEMVSRVRDREASRILIEGGAQKSLGPASGPMGTTVTVENLFFNTPARR
                     RFLKSPAREVAVIIEAVEALALAAPDVAFRLIDEGREVLWYPPEEFADRARRVLGPRV
                     AAHTLELSSRGAVDLEGILGTPQVAQPRRTHQRFLVNRRPVRSPLLARALTEAYHTLI
                     PDDRYPVAALALRLPPQEVDANVHPRKAEVRFVQDRRVFEAVVGEVRRALHRGPLAHV
                     IPAPGAGATTRAAGLDEGSGAGQPAVHDAPRGGVLLAAPAFDAPASAPARWPSIRVIG
                     QLALTYIVGEARGDLVLIDQHAAHERVLFEQLLARRTSAGRWAQGLVTPVVLDLAPRE
                     VEVLPELTPALRAIGFEVEPFGPAIVRLTAVPAIAVGRAPEELFRACLHDLEDDGGPH
                     AGRSLEERIAIATACHTAVRAGDPLDLTKMTDLVGALGQTTDPFSCFHGRPTMIQVRA
                     GDLERWFYRRT"
     gene            complement(3047..5710)
                     /gene="mutS"
                     /locus_tag="VEW91_05340"
     CDS             complement(3047..5710)
                     /gene="mutS"
                     /locus_tag="VEW91_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYM91041.1"
                     /translation="MSELTPMMRQYQALKDQHPHAILLFRLGDFFEAFFEDAHVVARE
                     LQLTLTSRPVAKGRRIPMCGIPHHALHSYLRRLIDRGHRVAICDQVEDPHLARALVRR
                     EVVRIVTPGTVVEDDLLSARENNFLAAVASSGEEWGAAFADLSTGEFFATQGAAPDHL
                     RATLALWHPRELLAPDPPGAGVVMEDVTTTAYDAERFEPPVAARALREHLGVATLDAF
                     GLTEAPLATAAAGALIQYLRETQKGPLPHLRAVRLIPRSSGMVIDDAAERALGLWSGG
                     ATLLSVLDLTETPMGARLLRRWLQQPLLDLAQITARLDAVDAFVSNARAREALRNRLR
                     SLGDVQRLAGRLSQAAGTPRDLAALRATLEELPTIAPILAEDSAAAIRSLAPALAVPP
                     DLVDLLRRGLVDAPPLTPRDGGMIRPGFDPEIDTLHEGTRAAREWIAGLEAAERARTG
                     IRSLKVGYNQVMGYYIEVSKANERLVPADYVRKGTLVGAERYITAELKDREALILTAQ
                     ERIAAREYEVFCQLRDAVRAQIASLMAAGRALAELDVFAALAEAAVRHRYVRPRPGEG
                     RLLRIVEGRHPTVEQAIGTERFVPNDLTVGTDDRDILIVTGPNFGGKSTYIRQAALIA
                     LMAQVGSFVPAREAEVGLVDRIFTRVGAADDLAAGRSTFLNEMIEVARILTQATPRSL
                     VILDEVGRGTSTYDGMSLAWAVVEDLHDRVGARTLFATHYHELTELASQLARCANLQV
                     LVRDEGQDIVFLHRVAAGAAASSYGIHVARLAGVPEMVIHRAREVLGGLEATAAGQRT
                     SRQRRGPSRSRQLALSLPSPVEEALRRADLARMTPLEALNFLSSLRALLERPGEGAHP
                     ADAPGAGSPAGGRPASTVIPFPKPPGGPPRP"
     gene            5934..6557
                     /locus_tag="VEW91_05345"
     CDS             5934..6557
                     /locus_tag="VEW91_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91042.1"
                     /translation="MWERTHLEQTMFALARDHAEAAGRCARATPPDREGERTHSLVSL
                     LMSFLALEAFINMVGGDRLGSRYRHYDRMSPEGKWLEVTRHVSKTGRTFGEDGKELRA
                     LSTLRSWRNMLTHYKGEYEEVQQAGRGGETTMDALLSAENAAQAVEIARTLYRAFYEM
                     DRRSPPRQFIWLDDRPHRARATSVAPSPGPAPGGMGAPRPHRRRRRR"
     gene            6571..7509
                     /gene="miaA"
                     /locus_tag="VEW91_05350"
     CDS             6571..7509
                     /gene="miaA"
                     /locus_tag="VEW91_05350"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYM91043.1"
                     /translation="MGSASLVVLAGPTAVGKTPVAIALAERVRGEIVCADSRTLYRGM
                     DIGTDKPTARDRERVPHYLLDVARPDEVVTLAAYQRLALGAIAEIRRRGRLPLLVGGT
                     GLYIRAVVDGLQIPPAAPDWSLRAALEAEERAGGPGSLHGRLREVDPAAAVRIHPRNL
                     RRIIRALEVHAVTGVPISVLQQSSAGPPPGAEEGSVVMIALTTDRVRLYERIHHRIDQ
                     HLAAGLVEEVRALVRAGYPKSLPALQGLGYKELIPYVEGSLSLGEAKALLQRNTRRYA
                     KRQLTWFRSDPRYRWLDVGADPPEVVAGRIRAMIWI"
     gene            7506..8690
                     /locus_tag="VEW91_05355"
     CDS             7506..8690
                     /locus_tag="VEW91_05355"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HYM91044.1"
                     /translation="MNLARRMRSIPPYLFADLDRKRAALRAKGVDVINLGIGDPDLPT
                     PPHIIDALAAAARDPATHTYPPYEGTQEFRGAVAAWYGRRFGVTLDPGREVLTLIGSK
                     EGLAHIPWVFVDPGDVALVSDPGYPVYAVATLMAEGEPHPVPLDPARGWLPDLTAIPD
                     ATARRARVFFLNYPNNPTAATADLAFFEEVVAFARPWGILVVHDNTYSEIAYDGYRPP
                     SFLQAPGAMEVGIEFHSLSKTYCMTGWRLGFVVGNRDAVQALATLKTNIDSGQFTAIQ
                     AAGTAALTGPQDVVRDRVARWEQRRNMAVQGLRAAGLDVPMPRATFYLWVRVPPGFDS
                     VGFAAHLLEHAGVLVTPGIGYGVRGSGHIRISLTAPDERIAEAIRRIGERLGVATPPA
                     TR"
     gene            8823..10052
                     /gene="hflX"
                     /locus_tag="VEW91_05360"
     CDS             8823..10052
                     /gene="hflX"
                     /locus_tag="VEW91_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYM91045.1"
                     /translation="MLVGLDSAGNGRGTLEELARLTQTAGATVAGVIVQRRTRPDPAT
                     FVGRGKVDAILARVRETGADLVIFDEELSPAQQRNLERSLGTKVLDRTALVLDIFAQR
                     ARTREGSLQVELAQMTYLLPRLSGRGVVLSRLGGGIGTRGPGETKLEADRRRIRARIT
                     TLQRAIGTLRQHRRQQRQARHAAALPVVALIGYTNAGKSSLLNSLTDAHVVTADKLFA
                     TLDPTVRKALLPNHRPVLFVDTVGFIQKLPHDLVAAFRGTLEEVVDADLLVHVIDGSH
                     PGWAEQVVAVEEVLHDLGADATPQIRAVNKVDRIPPEDLRDILAELPDAVPISALRQV
                     GLLNLLRRISQRLPDPAHRITLVIPYAEGRVLSQVYAHGRVLRRQDGDNAMTVEVEVP
                     LTAVERLRPYMRNPESA"
     gene            complement(10057..10686)
                     /gene="lexA"
                     /locus_tag="VEW91_05365"
     CDS             complement(10057..10686)
                     /gene="lexA"
                     /locus_tag="VEW91_05365"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYM91046.1"
                     /translation="MGGVTVGKGLTKRQREILTYVMTNMQQRGYPPSVREIGAALGLT
                     SSSTVHSHLTALEKKGFIHRDPSKPRAIEILKDGASQPPKRVVNVPVLGRIAAGQPLL
                     AEENVEDVFPLPRDLVREDAAFILRTRGDSMIEAGILDGDYLVIRQQATANNGEIVAA
                     LLGEEATVKRFYRERDHVRLQPENRTMTPILTREATILGRVVALIRRYP"
     gene            complement(10785..>11207)
                     /locus_tag="VEW91_05370"
     CDS             complement(10785..>11207)
                     /locus_tag="VEW91_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM91047.1"
                     /translation="LTEEAWDRILAVNVKGTFFCSRAAARVMTEGRIINIGSVAGVRG
                     TGSSIAYAASKGAIHTMTLSLARALAPRITVNAIAPGLIETRWHAGREVENARMVESI
                     PARRIGRPQDIAHVAVALASSDNFLTGQIIVVDGGALL"
BASE COUNT         1638 a         3969 c         3974 g         1626 t
ORIGIN      
        1 ggaaattccc agcgattcca catcatcacc cagggctgcc agatgaacgt ccgggattcc
       61 caggcgatgg ccggcctgct cgccggcatg ggataccagc cgacggagga tcccggcgag
      121 gccgacatca tcctgctcaa cacctgcacc gtccgcgagg gcgccgatga cagggcttat
      181 ggccggctgg gcgagttgca tgcgctcaag cggaggcgcc ccgggctgat cctcgggatc
      241 gccgggtgcc tcgtccagaa ggaccgggag cgagtgcggg agcgggctcc gtatctcgac
      301 ctggtgtttg gcgtccacaa catccaccgc cttcccgaac tgctccgcca ggttagggac
      361 ggctgtctgc ccgtgtacga ggtgtgggat cgctccgacc gggaccgccc cctgcccgtc
      421 ccgccggccc tccgggggga tcccatccgg ggattcgtca acatcatcca cgggtgcaac
      481 aaattctgca ccttctgcat tgtgccgtac gtgcgggggc gcgagcggag cgccccgccc
      541 gaagccgtcg cggcggaggt cgaagggctg gcggcgcagg ggtactgtga ggtgacgctc
      601 ctcgggcaga acgtggacag ttacggtcac gatctcaccc cacggcgcga tctcgcggat
      661 ctgctccgat tggtgcacga cgtcgacggc atcgagcgga tccgatttac gaccagccat
      721 ccgcgggata tgaccctgaa gctgatccgg acggtggcgg aactaccgaa ggtgtgcgag
      781 cacatccacc tgcccgtaca ggcgggagac gaccggctgc tccggcggat gcaccgggcg
      841 tatacgacgg cgcaataccg cgcaacgatc gacgccatcc gcgcggcgat gccgcaggca
      901 agcgtaacca ccgacgtcat cgtcggcttt cccggggaga ccgacgagga gttcgagggc
      961 acggtgaggc tggtcaacga ggtccaattt gacgcggtga acacggcgat atactcgccc
     1021 agggaaggca cccccgcggc cgggtatccc gaccaggtgc cggaggcgac caagcggcgg
     1081 cggctccacg cgctcaatgc gctggtggag cggatcgcag ccgaggtcaa ccgggcgctg
     1141 gtcggcacgc gtcaggaggt gctggtggag ggccccggga cccgcggggg catggtcggc
     1201 cgcacccgga cgaacaaggt ggtgatcctg gacggggacg ccgggtggat tggccggacg
     1261 gttccggtgg agattgcctc ggccggatcg tgggtgctcc gcggccgggc cggctctcag
     1321 gtcctccggt agaaccaccg ctcgaggtcg ccagcccgca cctgaatcat cgtggggcgc
     1381 ccgtgaaagc acgagaacgg gtccgtggtc tgccccagcg ccccgaccag atccgtcatc
     1441 ttcgtcagat ccagcgggtc tccggcccgc acggcggtgt ggcaggccgt ggcgatcgcg
     1501 atccgctcct cgagcgagcg cccggcgtgc ggcccgccgt cgtcctccag gtcgtgcagg
     1561 caggcacgga acagctcttc aggggctcgc cccaccgcga tcgccgggac ggccgtcagg
     1621 cgcacgatcg ccgggccgaa cggttccacc tcgaacccga tcgcgcggag cgcgggcgtt
     1681 aactcgggaa gcacctccac ctcgcggggc gcgagatcga ggacaacggg ggtgaccagc
     1741 ccctgcgccc accgccctgc gctcgtccgg cgggccagca actgctcgaa gagcacgcgc
     1801 tcgtgcgcag cgtgctggtc gatcaggacc aggtcgcccc gggcttcgcc gacgatgtag
     1861 gtgagcgcga gctgcccgat cacgcggatc gacggccagc gtgccggggc ggacgcgggc
     1921 gcatcgaacg ccggggcggc cagcaggacg ccccctcggg gcgcatcgtg caccgccggc
     1981 tgccccgccc cggacccctc gtcgagcccg gccgcccggg tggtggcccc cgcccccggg
     2041 gcggggatga cgtgcgccag cgggccccga tgaagagcgc gcctaacctc gccgaccacg
     2101 gcctcgaaca cccgtcggtc ctggacgaat cggacctcgg cctttcgggg atggacgttg
     2161 gcgtccacct cctggggcgg aaggcgaagc gcgagcgctg cgaccgggta ccggtcatcg
     2221 gggatcaggg tgtgataggc ttctgtgagc gcgcgggcga ggagggggct gcggacaggc
     2281 cgccggttga cgaggaatcg ctggtgggtc cggcgcggct gagcgacctg gggggtgccg
     2341 aggatccctt cgaggtccac ggccccccgc gaggagagtt cgagggtgtg ggcggccacg
     2401 cgcggaccca gcacacgcct ggcccggtcg gcgaactcct cgggggggta ccagaggact
     2461 tcacgcccct cgtcgatgag gcgaaacgcc acatcgggtg ccgcgagcgc gagcgcctcg
     2521 acggcctcga tgatgaccgc cacctcccgc gccggggact tcagaaaccg ccggcgggcc
     2581 ggcgtgttga agaagagatt ctccaccgtg accgtcgtcc ccatagggcc gctcgcgggc
     2641 ccgagggact tctgcgcgcc cccctcgatc aggatccttg acgcctcgcg atcccggacg
     2701 cgcgagacca tctcgacgcg cgagatagcc gcgatgctgg ggagggcctc cccgcgaaac
     2761 ccgtacgtgt ggattccctg caggtcttct gcggtccgga tcttgctggt ggcgaatcgc
     2821 tggaacgcca gggggaggtc ctcggcgggg attccctcgc cgtcgtcggt gacgcgcatg
     2881 agccggcggc cagcgccccg gacctcgacc gtgatccgcc gcgcgccggc atcgaggctg
     2941 ttctcgatca actccttgac caccgacgcc gggcgctcta ccacctcccc ggccgcgatt
     3001 cgctcggccg cgctgggggg aagaatccgg atgccccgat cgctgctcac ggccggggtg
     3061 ggccgccggg aggcttgggg aacggaatca ccgtgctggc gggccgcccg cccgccgggg
     3121 aaccggcccc aggggcgtcg gcggggtggg ccccttcgcc tggtcgctcg aggagcgccc
     3181 gcaggctcga caggaagttg agggcctcga gcggggtcat cctggcgaga tccgcccgcc
     3241 gcagcgcctc ctccacgggg gaggggaggg atagggccag ttgccggctc cgggaaggtc
     3301 cccggcgctg ccgggacgtc cgctggccgg ctgcggtcgc ttcgaggccg ccgagcactt
     3361 cgcgggcccg gtggatgacc atctcgggca ccccggccag gcgggcgacg tggatgccgt
     3421 aggaggacgc cgccgcgccc gccgcgaccc ggtggaggaa gacgatgtcc tgcccttcgt
     3481 ctcggaccag gacctggagg ttggcgcacc gggcgagctg cgaggcgagc tcggtgagtt
     3541 cgtggtagtg ggtggcgaag agggtgcgtg cgcccacgcg gtcgtgcaga tcctcgacca
     3601 cggcccacgc gaggctcatg ccgtcatagg tgctggtgcc ccggcccacc tcgtccagga
     3661 tgacgaggct tcggggggtg gcctgggtga ggatgcgcgc cacctcgatc atctcgttta
     3721 ggaacgtgct gcggcccgcg gcgaggtcgt ccgccgcccc gacccgggtg aagatccggt
     3781 caacgaggcc cacctcggcc tcccgggcgg gcacgaagct gccgacctgc gccatcaggg
     3841 cgatcagggc cgcctggcgg atgtaggtgc tcttgccgcc gaagttgggc ccggtcacga
     3901 tgaggatgtc ccgatcgtcg gtgccgaccg tcagatcgtt tgggacaaac cgttccgtcc
     3961 cgatcgcctg ctcgacggtg gggtgccgtc cctcgacgat ccgcagcaac cgcccttccc
     4021 ccggccgcgg ccgcacgtac cggtgccgga cggcggcctc ggcaagcgca gcaaacacgt
     4081 cgagctccgc caacgcccgg cccgccgcca tgagcgacgc gatctgcgcg cggacggcgt
     4141 cccggagctg gcaaaacacc tcgtattccc gagcagcgat tcgttcctgc gcggtcagga
     4201 tcagcgcctc gcggtccttc agttccgcgg tgatgtaccg ttccgcgccg accagcgtgc
     4261 ctttccggac atagtccgcg ggcaccagcc gctcgttggc cttgctgacc tcgatgtagt
     4321 agcccatcac ctggttgtag cccaccttga ggctccggat ccccgtccgc gcccgctcgg
     4381 ccgcctccaa cccggcgatc cactcgcggg ccgcccgggt tccctcgtgc agggtgtcga
     4441 tctccggatc gaacccgggg cggatcatcc cgccgtccct gggcgtgagg gggggcgcat
     4501 cgaccaggcc gcgccggagc aggtcgacca ggtctggcgg gaccgcgagc gccggcgcaa
     4561 gcgagcggat cgcggcagcg gaatcctccg ccaggatcgg cgcgatcgtc ggcagctcct
     4621 cgagggtagc gcggagggcg gcgaggtctc gcggcgtccc ggccgcttgg ctcaggcggc
     4681 cggcgagccg ctggacgtcc ccgaggctcc ggaggcggtt gcggagggct tcgcgcgccc
     4741 gggcattcga gacgaaggcg tcgaccgcgt cgagccgggc agtgatctgc gccaggtcga
     4801 gcagtggctg ctggagccag cgcctgagaa ggcgcgcccc cattggcgtc tccgtcaggt
     4861 cgagcacgct gaggagtgtc gcgccccccg accacagccc gagcgctcgc tccgcggcat
     4921 cgtcgatgac catcccggac gatcgcggga tcaggcggac ggccctcagg tggggcaggg
     4981 ggcctttctg cgtctcccgg agatactgga tgagcgcccc ggcggcggcg gtggccagcg
     5041 gcgcctcggt caggccgaac gcgtccaggg tggcgacccc gaggtgctcc cgcagcgctc
     5101 tggccgcgac cggtggctcg aaccgctcgg catcgtacgc cgtggtcgtc acgtcctcca
     5161 tgacgacgcc ggccccgggg ggatcgggcg cgagcagttc gcgcgggtgc cagagcgcca
     5221 gcgtggcgcg gaggtggtcc ggggcggccc cctgggtcgc gaagaactcg ccggtggaca
     5281 ggtcggcaaa cgccgccccc cattcctcac cgctcgaggc gacggccgcg agaaagttgt
     5341 tctcgcgcgc gctgagcagg tcgtcctcga ccacggtgcc cggggtcacg atgcggacga
     5401 cctcccggcg gacgagcgcc ctggcgagat gcgggtcttc gacctgatcg cagatcgcca
     5461 cgcgatgccc gcggtcgatg aggcggcgca ggtagctgtg cagcgcatgg tgcgggatgc
     5521 cgcacatcgg gatccggcgc cccttcgcca cgggccggct ggtcagggtc agctgcagct
     5581 ctcgcgcaac gacgtgggca tcctcgaaga acgcctcgaa gaagtccccg aggcggaaga
     5641 ggagaatggc gtggggatgc tggtccttga gcgcctggta ctggcgcatc atcggcgtga
     5701 gctcgctcat gggtcccatt cttcttcgtc gcgcgccggc agggtccccc tcggggccgc
     5761 cgaactgaag tagtatgcca cgccgccgcc gtcgtcgctc cgcgatcccg accccgactc
     5821 cgcctcccgc cgggggggcg cccgcagggg cgcccgcgcc ggccgcccca cccgctgccc
     5881 cgccggttgc cccgcccgcc gcgccccctc ccgcgcccga tgcgggggaa caggtgtggg
     5941 agcgcaccca tctcgagcag acgatgtttg ccctggcccg cgaccacgca gaggcggcgg
     6001 gtcggtgcgc ccgcgcaacg ccgccggacc gcgagggcga gcggacgcac agcctagtct
     6061 cgctgctgat gtccttcctc gcgctcgagg cgttcatcaa catggtcggc ggtgaccggc
     6121 tggggagccg gtatcgtcat tacgaccgca tgtcgcccga ggggaaatgg ctggaagtca
     6181 cgcgccacgt gagcaagacg gggaggacgt tcggcgaaga cggcaaggaa ctgcgggcgc
     6241 tgtcgacgct gaggagctgg cgaaacatgc tcacgcatta caagggggag tacgaagagg
     6301 tgcagcaggc gggccggggc ggcgagacca cgatggatgc gctgctctcc gcggagaacg
     6361 ccgcccaggc cgtggagatc gcgcgcaccc tataccgcgc cttctacgag atggaccgcc
     6421 ggtccccccc acgccagttc atctggctcg acgaccgccc gcaccgggcg agggccacga
     6481 gcgtcgcccc atcccccggc ccggccccgg gcggcatggg agccccccgg ccccatcggc
     6541 ggcggcggcg ccgctgaggc gtacggcgcc atgggaagcg cgagcctcgt cgtgctcgcg
     6601 ggcccgaccg ccgtagggaa gacgcccgtg gcgatcgccc tcgccgagcg cgttcggggg
     6661 gagatcgtct gcgccgactc gcggaccctg taccgcggga tggatatcgg gacggacaag
     6721 cccacggcgc gggaccggga gcgggtaccg cactatctgc tcgacgtcgc gcgccccgat
     6781 gaggtcgtca ccctcgccgc ctaccagcgc ctcgccctgg gagcgatcgc ggagatccgg
     6841 cgccgcggtc gcctcccgct gctcgtgggc gggacgggcc tgtacatccg ggcagtggtg
     6901 gacggcctcc agatcccgcc cgcggcgccc gactggagcc tgcgcgcggc cctggaggcc
     6961 gaggagcggg ccggcggccc gggctcgctg cacggacggc tgcgcgaggt cgacccggcg
     7021 gcggcggtgc gtattcatcc gcgcaatctc aggagaatca tccgcgccct cgaagtgcac
     7081 gccgtgaccg gcgtcccgat ctccgtcctc cagcagtcgt cggccggccc gccccccgga
     7141 gcggaggagg gatccgtggt gatgatcgcg ctcaccaccg accgggtccg gctctacgag
     7201 cgcatccatc accggatcga ccagcacctg gcggccgggc tggttgaaga agtgcgggcg
     7261 ctcgtgcgcg ccgggtaccc gaagtccctg ccggcgcttc aggggttggg gtacaaggag
     7321 ctcatcccct atgtggaggg gagcctgtcc ctcggggagg cgaaggccct gctgcagcgc
     7381 aacacccgcc gctacgccaa gcgccagctg acctggttcc gaagcgaccc ccgctaccgg
     7441 tggctagatg tgggggccga tcccccggaa gtggtggccg gccgaatccg tgctatgatt
     7501 tggatatgaa cctcgcgcgc aggatgcgca gcatcccacc gtatctgttc gccgacctgg
     7561 accggaagcg cgcggcgctc cgggccaagg gcgtggatgt catcaacctc ggcatcggcg
     7621 atcccgacct gcccactccc ccgcacatca tcgacgcgct ggccgcggcc gcccgcgatc
     7681 cggccaccca cacgtatccg ccgtacgaag gcacccagga gttccggggg gccgtcgcgg
     7741 cctggtatgg ccggcggttt ggggtcaccc tcgaccctgg ccgcgaagtc ctcaccctga
     7801 tcggctcaaa ggaagggctc gcccacatcc cctgggtgtt cgtcgaccct ggggatgtcg
     7861 cactcgtcag cgatcccgga tacccggtgt acgccgtggc caccctcatg gccgaggggg
     7921 agccgcaccc ggttccgctc gaccccgcgc gcgggtggct gccggacctt acggccatcc
     7981 ccgacgccac ggcgcgtcgc gccagggtgt tcttcctcaa ctaccccaat aacccgaccg
     8041 ccgccaccgc cgatctggcc ttcttcgagg aggttgtcgc gttcgcgcgc ccgtggggca
     8101 tcctggtggt ccacgataac acgtactcgg agatcgccta cgatggctac cggcccccga
     8161 gttttctcca ggcccccggg gcgatggagg tcggcatcga gttccattcc ctcagcaaga
     8221 cctactgcat gaccgggtgg cggctcgggt tcgtggtcgg gaatcgcgac gccgtgcagg
     8281 ccctggccac gctcaagacc aacatcgaca gcgggcagtt caccgcgatc caggcggccg
     8341 gcaccgccgc cctcacgggt ccccaggacg tcgtccgcga ccgtgtggcc aggtgggagc
     8401 agcggcggaa catggcggtc cagggtctca gggccgccgg gctcgacgtc ccgatgccgc
     8461 gcgccacgtt ctacctctgg gtgcgagtgc cccccgggtt cgactcggtg gggttcgccg
     8521 cgcacctcct cgagcacgcg ggcgtgctgg tcacgcccgg catcgggtac ggtgtgcggg
     8581 ggagcggcca tatccgcatt tcgctgaccg cccccgacga gcggatcgcc gaggcgatcc
     8641 ggcgaatcgg cgagcggctg ggggtagcca cgccgcccgc gacgcggtag gggaggggac
     8701 cgatccgaac gacaggggcg ccccagggcc gcaaactccg ccggggtctc cacccaccac
     8761 agcgccgtga gccgtaccag gctcctccgc gtggggtcgc gagtggagca ccagagtccg
     8821 cggtgctggt cgggctggac tcggccggga acggacgcgg cacgctcgag gagctcgccc
     8881 gactcaccca aaccgccggg gccaccgtgg ccggcgtcat cgtccagcgc cgcacccgcc
     8941 ccgatcccgc gaccttcgtc ggccgcggca aggtcgacgc gattttggcg cgcgttcgtg
     9001 agacgggcgc ggacctcgtc atcttcgacg aggaactctc ccccgcccaa cagcgcaacc
     9061 tggagcggtc cctgggcacc aaggtgctcg atcgcacggc cctcgtgctg gatatcttcg
     9121 cgcagcgcgc gcggacacgc gagggttcgc ttcaggtcga actcgcgcag atgacgtacc
     9181 tgctgccccg gttgagcggc aggggagtgg tgctgtcacg tctcgggggc ggcatcggca
     9241 cccgtggccc cggcgagacc aagctggaag ccgaccggcg gcggatccgc gcgcgcatca
     9301 ccaccctcca gcgcgcgatc ggcaccctgc gccagcaccg ccggcagcag cggcaggcgc
     9361 gccatgccgc cgccctcccc gtcgtagcgc tcatcggcta caccaacgcc ggcaagtctt
     9421 ccctcctcaa cagcctgacc gatgcgcacg tcgtgaccgc ggacaagctg ttcgccaccc
     9481 tcgaccccac cgtgcggaag gcgctgctgc ccaaccaccg gccggtgctg tttgtcgata
     9541 ccgtggggtt catccagaaa ctgccgcacg atctcgtggc cgcgtttcgg gggacgctgg
     9601 aagaggtggt ggacgcggac ctgctggtcc acgtgatcga tggcagccac cccggatggg
     9661 ccgagcaggt ggtggcggtg gaggaggtac tgcacgacct cggggcggac gccaccccac
     9721 agatccgcgc ggtcaacaag gtggaccgga tccccccgga ggatctccgg gatatcctgg
     9781 cggaactccc cgacgcggtg ccgatctctg cgctgcggca ggtcgggctg ctgaacctgc
     9841 tgcgtcggat ctcgcagcgg ttgccggacc cggcccaccg gatcacgctc gtcattccgt
     9901 acgcggaggg acgggtgctc tcgcaggttt acgcgcacgg gcgcgtcctg cggcggcaag
     9961 acggggacaa cgcgatgacg gtggaggtcg aggtgccgct gaccgcggtc gagcggctca
    10021 gaccctacat gcggaacccg gagagcgcct aggggtttac gggtagcgcc ggatcagtgc
    10081 cacgaccctc cccaggatcg tggcctcccg ggtcaggatc ggagtcatcg tccggttctc
    10141 gggttgaagg cggacgtgat cgcgctcccg gtagaagcgt ttgacggtgg cctcctcccc
    10201 caacagggcc gccacgatct ccccgttgtt cgccgtcgcc tgctgccgga tgacgagata
    10261 gtccccgtcg aggatcccgg cctcgatcat gctgtctccc cgcgtgcgga ggatgaacgc
    10321 ggcgtcctcc cggacgagat cccgggggag ggggaagacg tcctccacgt tctcttcggc
    10381 caggaggggt tggccggcgg cgatccgccc caacacgggc acattgacga cgcgcttggg
    10441 gggctggctc gccccgtctt tcaggatctc gatcgctcgg ggcttgctgg gatcccggtg
    10501 gatgaacccc tttttctcca gcgcggtgag gtggctgtgg accgtcgagc tgctcgtcag
    10561 cccgagcgcc gccccgatct cgcgaacaga cggcggatag ccgcgctgct gcatgttggt
    10621 catcacgtag gtcaggatct cacgctggcg tttcgtcagc cccttgccca cggttacgcc
    10681 ccccaacgct ccccgtctcg gtcacccggc ggcccgcatt ataacacaac catgccaagg
    10741 atgcaaacac ctgttcgcca ttcggccact gatccccgct ccctttagag caaggcgccg
    10801 ccgtcgacga cgatgatctg ccccgtgagg aagttatctg aggaggcgag cgctactgcg
    10861 acgtgtgcga tgtcctgagg gcgcccgatg cgcctggccg gaatcgattc caccatcctt
    10921 gcgttctcga cctcgcgccc ggcgtgccag cgggtctcga tgagcccggg ggcgatcgcg
    10981 ttgactgtga tcctcggcgc cagggcccgc gcgagggaca gggtcatcgt gtggatcgcc
    11041 cctttgctgg ccgcgtacgc gatggagctc cccgtcccgc gcacccccgc gaccgatccg
    11101 atgttgatga tccgcccctc ggtcatcacc cgcgcggcgg cgcggctgca gaagaacgtg
    11161 cctttgacgt tcaccgccag aatccggtcc cacgcctctt cggtcag
//
LOCUS       DASQGH010000274         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54976, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000274 DASQGH010000000
VERSION     DASQGH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54976"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VEW91_05375"
     CDS             complement(<1..509)
                     /locus_tag="VEW91_05375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYM91048.1"
                     /translation="MATFEQVSIESIKDFWDRRSCNIRHSSKPVGTREYFDEVEARKY
                     YVEPHIPRFAQFERWQGKRVLEIGCGIGTDTINFARRGARVTAVELSEKSLELARQRA
                     DVYGLEDRIQFHLGNAEELADFVPVEPYDLVYSFGVIHHTPHPQRVIGQIRRYVRSDS
                     VVKLMVYHR"
     gene            complement(509..1519)
                     /locus_tag="VEW91_05380"
     CDS             complement(509..1519)
                     /locus_tag="VEW91_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYM91049.1"
                     /translation="MSTRILVSGAGGFIGHHLVNRLKERGYWVRGVDLKPPEYEPTRA
                     DEFKTLDLRRWNECLEATRDIDECYHLAANMGGIGFITAHKAEIMRDSVLINTHMLEA
                     ARTHKVTKFLFTSSACVYPGYRQNVPDVTPLKEEDAYPADAEDGYGWEKLFTERMCRH
                     YHEDYGLRTYMVRFHNIYGPLGTYDGGREKSPAAICRKIALVKNGGEIEIWGDGKQTR
                     SYCYIDDCVEGLFRLMQTSEHAPVNLGSERLVSIDELVDLVARIAGKRVTKRHDLTKP
                     QGVRGRNSDNTKLRRVLGWEPRVSLEEGLARTYRWIREQLVRTGRIREEVAPRSNRRH
                     EG"
     gene            complement(1584..>1877)
                     /locus_tag="VEW91_05385"
     CDS             complement(1584..>1877)
                     /locus_tag="VEW91_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91050.1"
                     /translation="THILTQQGIVEEPPSRLKAKRAYYLKRLLVRVGVAHCLAGDIRT
                     AWGAWSEAVMRDPYVLKAPRLLFMATSILGSRMVRWSRRIRDSVMSAALGARP"
BASE COUNT          339 a          609 c          563 g          366 t
ORIGIN      
        1 tagcggtgat agaccatgag tttgaccacg ctgtcggacc ggacataccg gcgaatctgc
       61 cctatgaccc gctggggatg cggcgtgtgg tgaatgacgc caaaggaata gacgaggtcg
      121 taaggctcga cggggacgaa gtccgcaagt tcttcggcat tgcccaagtg aaactggatg
      181 cggtcctcca gcccatacac gtcggccctc tggcgggcga gctcgagcga cttctccgac
      241 agctcgactg cggtgacccg cgcgccacgg cgggcgaaat tgatcgtatc ggtcccgatg
      301 ccacatccga tttccagcac tcgctttccc tgccagcgct cgaattgcgc aaaccggggg
      361 atgtggggct ctacgtaata cttacgggcc tccacctcgt caaagtattc acgcgtccct
      421 acgggcttgg atgaatggcg aatgttacat gaccgccggt cccagaagtc tttgatcgat
      481 tcgattgaca cctgctcgaa tgtcgccatc atccctcgtg acgacggttc gagcgcgggg
      541 cgacctcttc ccggatccgt ccggtccgga ccaactgctc gcggatccac cggtaggttc
      601 gcgccaggcc ctcctccagg gacacccgag gctcccaccc cagcacgcgc cgcagcttcg
      661 tgttgtcgct attgcggccg cgcaccccct gcggcttggt gaggtcatgg cgcttggtca
      721 cccgcttgcc cgcaatccgc gcgaccagat cgaccaactc atcgatactc accagccgct
      781 cggagccgag gttgaccggc gcgtgctcgc tcgtctgcat cagcctgaag aggccttcga
      841 cacagtcgtc gatatagcaa taggagcggg tttgcttgcc gtcgccccaa atctcgatct
      901 cgccgccgtt cttgacgagg gcgatcttcc ggcagatcgc cgcgggagat ttttcccgtc
      961 ccccgtcata cgtgccgagg gggccgtaga tgttgtggaa ccggaccatg taggtgcgga
     1021 ggccgtaatc ctcgtggtag tggcggcaca tccgctcggt aaagagcttc tcccacccat
     1081 agccgtcctc ggcgtcggcc gggtaggcat cctcttcttt gaggggcgtc acatccggca
     1141 cgttctgccg atagccggga tacacgcacg ccgacgaagt gaagaggaac ttcgtgacct
     1201 tgtgggttct ggcggcctcc agcatgtggg tgttaatgag cacgctgtcc cgcatgatct
     1261 cggctttgtg ggcagtgatg aacccgatcc ctcccatgtt cgcggcgagg tggtagcatt
     1321 cgtcgatgtc ccgcgtcgcc tcgagacact cgttccatcg ccggaggtcc aacgttttga
     1381 actcatcggc ccgggtgggt tcgtactcgg ggggcttgag gtccaccccg cgaacccaat
     1441 agccacgctc cttcaaccgg ttcacgagat gatgtccgat gaaaccccct gccccgctga
     1501 ccagaatcct cgtgctcatc ggaaccctcc tcgagatcgt accggggccg cccacgcagg
     1561 actggaggtg gcgcgggaga cgctcatggg cgggcaccga gggcggcgga catgacgcta
     1621 tcccgtatcc ggcgagacca gcgcaccatc cgcgacccta agatagacgt ggccatgaag
     1681 agcaaccgtg gagccttcaa gacgtaagga tcccgcatca ccgcctccga ccacgcaccc
     1741 caggcggttc tgatgtcgcc cgcaagacag tgggccacgc caaccctgac cagtaacctc
     1801 ttgaggtagt aggcgcgctt tgccttcaac cggctgggcg gctcttcgac gatgccctgc
     1861 tgggtcagga tatgggt
//
LOCUS       DASQGH010000275         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52087, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000275 DASQGH010000000
VERSION     DASQGH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52087"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VEW91_05390"
     CDS             complement(<1..898)
                     /locus_tag="VEW91_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HYM91051.1"
                     /translation="MLERGFLPDFGAAVLTELGAIPGPTATQIPSVRELSGLLWASID
                     NDDSLDLDQLSVAEPLADGRVKVLVAIADVDAFVGRASAINAHAQQNATSVYTVAQVF
                     PMLPERLSTDLTSLREGQERLAIVIEMETDPDGAVVGSDLYRATVVNHGKLAYNAVAA
                     WLAGQGPMPPRVAVVPGLADQLRLQDRVAQALRARRLHHGALTLQTIESQAVFDGDAL
                     TDLQPVQQNRAEALIEDLMIAANGVTARYLEARGVPAFRRVLHVPERWDRIVELAAGF
                     GEHLPGAPDAAALEAFLARRQQA"
     gene            complement(1180..>2771)
                     /locus_tag="VEW91_05395"
     CDS             complement(1180..>2771)
                     /locus_tag="VEW91_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYM91052.1"
                     /translation="LCSDKTGTLTSGEMVLDQHLDPLGQSTDRVLLLASLNSAYETGI
                     KSPLDEAILKRGVRPPMGATTPDHDGTGGTGYRKLDEIPFDFERRRLSVVVEKDGERL
                     LITKGAPEGVLVQTTAYEADGQQHPFDAAARPRIEGVYRKLSEDGFRVLAVAYRAVPQ
                     QEAYRTADERELVLAGFLAFIDPPLEDAVDVLTAMRRDGVTVKILTGDSDLVARHVCT
                     KVGLEPGRIVLGEELARMTDPALGQVAEGTTIFARVSPAQKNRILLALKRRGHVVGFL
                     GDGINDAPSLHAADVGISVASGVDVAKDAADIILLERSLQVLHQGILEGRKAFGNVMK
                     YLLMGTSSNFGNMFSMAGASVFLPFLPMLPTQILLNNFLYDFAQVTIPTDNVDETFMQ
                     KPRKWNIATIRDFMIVIGPISSVYDFLTFYVLLNVFHASPTLFHTGWFVESLATQTLV
                     IFVIRTAGNLLLSRPSPALAATTLLVVLIGILLPLSQLAGPLGFVPLPGGFFAFLAAV
                     TITYLLLVEMAKRRLMRRLMV"
BASE COUNT          502 a          895 c          888 g          486 t
ORIGIN      
        1 cggcctgctg ccgcctggcg aggaatgctt cgagcgcagc cgcatcaggg gctcccggaa
       61 ggtgttctcc gaaccccgcc gccagctcga cgatccgatc ccagcgctcg ggaacatgca
      121 ggacgcggcg gaacgccgga acacctctgg cttcgagata gcgggctgtc accccattcg
      181 ccgcgatcat gaggtcctcg atcagcgctt cggcccgatt ctgttgcacc ggctgtagat
      241 cggtgagcgc gtcgccgtcg aatacggcct gcgactcaat tgtctggagc gtcagcgcgc
      301 cgtggtggag tcgccgcgcc ctcagcgcct gcgcgacccg gtcctgcagg cgaagctggt
      361 ccgccagccc gggaacgacc gcaacccgcg gaggcatcgg tccctgcccc gcgagccacg
      421 ccgccacggc gttgtacgcg agcttgccgt ggttgaccac cgtggcccga tacagatccg
      481 accccaccac ggcgccgtcc gggtcggtct ccatctcgat gacgatggcc agtcgctcct
      541 ggccctctct gagcgaggtc agatccgttg acagccgctc cgggagcatg gggaagacct
      601 gtgcgaccgt gtagacggac gtcgcgttct gttgcgcgtg cgcgttaatc gccgaggctc
      661 ttcccacgaa ggcatccacg tcggcaatcg cgacgaggac cttcaccctg ccgtcggcca
      721 ggggctcggc gaccgagagc tgatcgaggt cgagcgagtc gtcgttgtca atggacgccc
      781 agagcaatcc actcagctcg cggacggacg gaatctgcgt cgcggtaggt ccggggatgg
      841 cccccagctc tgtgagcacg gccgcgccga aatccggcag gaagccgcgc tcgagcatcg
      901 cgcgccgagc aattgtctgg aggtcgtgtc gactcgaggt tgtgggttta ctcatttgac
      961 gcttcgtcgg gtcgccagaa ggaggatggc tcatgggacg agcatcatca tgatcctgct
     1021 cgacgttggg ctgaacttcg tccaggcgta ccgcgcataa cgagcatctc tcctcctatg
     1081 ccatagtctg tctatcgaat gaggatctta ggggtgccat tcttggggag aagatcaaag
     1141 accccggtag atgcaccacg ttgttccaag acgcgctcct tacaccatga gccgtcgcat
     1201 gagacgcctc ttcgccatct cgaccaacag caagtaggtg atggtgaccg cggccagaaa
     1261 cgcgaaaaac ccccctggga gcggtacgaa ccccaacggg ccggccagct gggagagcgg
     1321 gagcaggatc ccgatgagga cgaccaggag ggtcgtcgcg gcgagggccg ggctcggccg
     1381 actcaggagc aggttgcccg ccgtccgaat gacgaagatc acgagggtct gagtggccag
     1441 cgactccacg aaccagcctg tgtgaaatag ggtcggggag gcgtggaaca cgttgaggag
     1501 cacgtagaac gtcaggaagt cgtaaaccga gctgatcggg ccgatgacga tcatgaaatc
     1561 ccggatcgtc gcgatgttcc atttccgcgg cttctgcata aaagtctcat cgacgttgtc
     1621 ggtggggatc gtgacctggg cgaagtcgta caggaagttg ttgagtagaa tctgggtggg
     1681 cagcatagga aggaacggca ggaacaccga cgctccggcc atgctgaaca tgttcccgaa
     1741 gttcgagctc gtccccatga ggagatactt catcacgttg ccgaaggcct tccgcccttc
     1801 gaggataccc tggtgcagca cttgcaggct gcgctcgagc aggatgatat cggcggcatc
     1861 cttcgccacg tcaacgccgc tggccaccga gatgccgacg tcggcggcgt gcagggaggg
     1921 ggcgtcgttg atgccgtcgc cgagaaagcc cacgacgtgg cctcgacgct tgagggcgag
     1981 caggatccga ttcttctggg ccggggacac ccgtgcgaag atggtggtcc cctcagcgac
     2041 ctggccgagc gccgggtcgg tcatgcgggc caactcctcc cccagcacga tacgacccgg
     2101 ctccaatcct acttttgtgc aaacatgccg agcaacaagg tcgctgtctc ctgtcaggat
     2161 cttcaccgtc accccgtccc gccgcatcgc ggtcagcacg tcgacggcgt cctcgagagg
     2221 cgggtcgatg aacgcgagga agccggcgag caccaactcc cgctcgtcgg ctgtccggta
     2281 agcctcttgc tgcgggacgg cgcggtaggc gaccgccagc acacggaacc cgtcctcact
     2341 cagcttccgg tacactccct cgatcctcgg ccgcgcggcg gcatcgaagg ggtgctgctg
     2401 cccatccgcc tcgtaggcgg tcgtctgaac cagcaccccc tcgggcgccc ccttggtgat
     2461 caggagacgc tcgccgtctt tttcgaccac caccgacagc cgccgccgct cgaaatcgaa
     2521 cgggatctcg tcgagtttcc gataccccgt accgcccgtc ccgtcatggt cgggggtcgt
     2581 ggcgcccatc gggggccgga cacctcgctt taagatggcc tcgtccaacg ggctcttgat
     2641 ccccgtttcg taggcgctgt tgagggacgc gagcagcaag acgcgatcgg tggattgtcc
     2701 cagcggatcc agatgctggt ccagcaccat ctccccactg gtcagcgtgc cggttttatc
     2761 gctgcagagg a
//
LOCUS       DASQGH010000276         1740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52089, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000276 DASQGH010000000
VERSION     DASQGH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52089"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VEW91_05400"
     CDS             complement(<1..1262)
                     /locus_tag="VEW91_05400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HYM91053.1"
                     /translation="MTRQADLARLRTPTFDVLVIGGGITGAGVALEAAARGLSVALVE
                     RGDFAQGSSSRSTKLIHGGLRYLPMLDIAQVREGLEEQNALLRNAPHLVRPTPFLVPL
                     YKGAVRPLGLDLPAPLRAALPIGIGMGLWGYDLLAGRRGARRHRRLGPAAAAALVPHL
                     RREGLRLAYLYYDGLTDDARLTLAVLQTAVRLGARAVNYLEATALVTEAGRITGARLT
                     DRLSGEHVAVSARTTINATGVWAEQVARLAGPPPAFRIRRAKGVHLVLPNDRLGMGRT
                     ALVLPETDDGRIAFVVPWQGALVVGTTDTEWDRAENEPEVMGDDVAYLLDHASRFLTV
                     PLSPRDVHSAFSGLRPLISTGTAASARLSRRHEVVPSAPGFYSIIGGKLTTFRRMAED
                     VVNVATGRRVGTPSRTRTVCLAGADGLA"
     gene            complement(1262..>1740)
                     /locus_tag="VEW91_05405"
     CDS             complement(1262..>1740)
                     /locus_tag="VEW91_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYM91054.1"
                     /translation="LCAELPGVRATVGRCEVSPNAINVIPGAVRLSTDLRARTADLLE
                     TAARRLEGLCTTVGRDDRVEVRTQEFWRSPPTPFHPTVTRAVAETARSLGYTTLPLWA
                     GAGHDAKYVADRYPAGMIFVPSAGGLSHNEAEWTPKADCVRGATVLLGTVLRLAAA"
BASE COUNT          251 a          625 c          639 g          225 t
ORIGIN      
        1 tcggccagac catccgcccc ggccagacag accgtgcgcg tcctggacgg cgtccccacg
       61 cgccggccgg tggccacgtt cacaacatcc tccgccatcc gccggaaggt ggtgagtttg
      121 cccccgatga tcgagtagaa cccgggtgcc gacggcacga cctcatggcg gcgggagagg
      181 cgcgcgctcg cggcggtgcc ggtgctgatc agcggccgca ggccggaaaa tgcgctgtgc
      241 acgtcgcggg gtgacagcgg gacggtgaga aaccgggagg cgtggtcgag caggtaggcc
      301 acatcgtccc ccatgacctc cggctcgttc tccgcccgat cccactcggt atcggtggtc
      361 ccgacgacca gcgccccctg ccacggtacg acgaacgcga tgcggccgtc atcggtctcg
      421 ggcaggacga gggcggtgcg acccatcccg agccggtcgt tgggcaggac gaggtggacc
      481 cccttggccc ggcggatgcg gaacgcggga ggagggccgg cgagacgggc cacctgttcg
      541 gcccagaccc cggtggcgtt gatcgtcgtg cgcgcggaga ccgcgacgtg ctcgccgctc
      601 aggcggtcgg tcagcctcgc tccggtgatc cgcccggctt cggtcacgag tgcggtcgcc
      661 tcgagatagt tgaccgcgcg ggcccccaga cgcacggccg tctgcagcac ggccagcgtc
      721 agccgggcgt catcggtcag gccgtcgtag tagaggtagg cgaggcggag cccctcgcgc
      781 cggagatggg gaacaagcgc cgcagccgcg gcggggccga gccggcggtg gcggcgcgcc
      841 ccgcgccggc cggccaggag gtcgtagccc cacaggccca tcccgatgcc gatcgggagg
      901 gccgcgcgaa gcggagccgg caggtcgagc ccgagcgggc gcaccgcgcc tttgtagagc
      961 ggcaccagga acggcgttgg ccggaccagg tgcggggcgt tgcggaggag cgcgttctgc
     1021 tcctcgagcc cttctcgcac ctgggcgatg tcgagcatgg gaaggtagcg cagcccgccg
     1081 tggatgagct tggtggaccg actgctgctg ccctgggcaa agtcccctcg ctcgaccagc
     1141 gccacggaca acccacgcgc cgccgcttcg agcgccaccc cggccccggt gatcccaccc
     1201 ccgatgacga gcacatcaaa cgttggcgtc cgcagccgag ccaggtccgc ctggcgggtc
     1261 atcacgccgc cgcgaggcgc aggacggtgc ccagcagcac ggtcgcgccg cggacgcagt
     1321 cagccttcgg ggtccactcg gcttcattgt ggctgagccc gccggcggac ggcacgaaga
     1381 tcatgccggc gggataccgg tccgccacgt acttcgcatc gtgcccggca ccggcccaca
     1441 gcggcagggt ggtgtacccg agggatcgtg cggtctcggc cactgcgcgc gtgaccgtcg
     1501 gatggaacgg ggtcggcggg ctccgccaga actcttgggt gcggacctcc acccggtcgt
     1561 ccctgcccac ggtcgtgcac aacccctcca gcctgcgcgc ggccgtctcg aggaggtccg
     1621 cggtccgcgc cctcaggtcg gtcgacagcc gcacggcgcc gggaatgacg ttgatcgcgt
     1681 tggggctcac ttcgcaccgg ccgacggtgg cgcgcacccc cggcaactcc gcgcagaggg
//
LOCUS       DASQGH010000277         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1208, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000277 DASQGH010000000
VERSION     DASQGH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..142
                     /locus_tag="VEW91_05410"
     CDS             17..142
                     /locus_tag="VEW91_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91055.1"
                     /translation="MYQRDYPVVQGAVMLFAATFLVTNLLVDLAYTYLDPRIRYE"
     gene            135..1034
                     /locus_tag="VEW91_05415"
     CDS             135..1034
                     /locus_tag="VEW91_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91056.1"
                     /translation="MSRTGVGVQDAGGGIGPPGTARRAEDPLRLARRNPVAAIGAGLI
                     AIVVLLALGANLIAPYGPVAQIAPPLLRPGHGFFAGTDELGRDELSRLIFGARVSLYV
                     GVLAVSIALAGGATSGIIAGFYGGWLDNALMRTMDILFSLPAIVLAIAITSILGPSLT
                     NAMIAIGIVYAPTFARIARGPTLAVMNLAYIEAARAIGAPNWTIILRHVLPNVSAPLL
                     VQTTVSLSTAILTEAALSFLGLGTQPPTASWGLMLNAARQYMLIDPWIAILPGVAIAL
                     TVLGFNLLGDGLRDLLDPRIRTM"
     gene            complement(1045..2343)
                     /locus_tag="VEW91_05420"
     CDS             complement(1045..2343)
                     /locus_tag="VEW91_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYM91057.1"
                     /translation="MRESPSTVADLMIPVPTPIPGAATVGEAIDLIRAHDHGGLPVVD
                     GARLVGFVTPIHLLRQPLYRTVAEVMATDLSPARPDLSIGQAYELLMRQRADVLPVVE
                     GGRLVGLITLAAVLEARSQERDPLTGLPWATALRTWATSALEGGREVAVLFIDLDNFR
                     IINKALGHVVGDDIIRSVAYLLASQIDPATDLLCRYAGDEFAVATTRGSEDARGLAQR
                     LRDAVNLPVEIQGREERVTVAIGFAGGRRVEPRTAAHIASTVEDLLTLASRGSTLAKE
                     SGRGIVHYSRREEDAAPRDGGADQRSDEVRLRLVRASVNRDVQGSTAVVELSLRGRAV
                     RGTASARILGHGAPFLVAEATLRAITQAVGESPGYLLDDLTLTPSEQDTVAVAVLISA
                     ADPSERLVGSAGAPDPHTAVSRAILSALNRRLGKALAGVL"
     gene            2623..3372
                     /locus_tag="VEW91_05425"
     CDS             2623..3372
                     /locus_tag="VEW91_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM91058.1"
                     /translation="MGKTHEAGLKMAERPKPPGARSRRTGGRRLRELRAAAGADERRR
                     LVQALHDGPLQMATAAKIRLQARRQGVGDRTAAKSLDEAIALIGQAIAAMRALLRDPA
                     PPSGSGSLQVRLRRAAGRWGEVTGVRVHLSVLGAARASAFSRETLEVAEHVVSESIVN
                     AWKHGRATRVSVRCERHQGGLLLTLQDDGRGFRPSIVPEPRNGTRMGLRLLRSRVDEL
                     GGWFDVRSSHGGGAVVQAWLPPRPASHRAPE"
     gene            3469..>5631
                     /locus_tag="VEW91_05430"
     CDS             3469..>5631
                     /locus_tag="VEW91_05430"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM91059.1"
                     /translation="MAVSQYVGARVKRIEDPKLIRGEGAYLDDLRLAGMLHAVFIRSP
                     HGHARIGGINLWRARAHPGVAGVLAGDDLREIQRPLPVMPVEGMRAADHLPLASGEVC
                     YAGEPVAVVVAEDPYIARDAADLVEIAYDPLPAAIDLERAAKGAPFAHAGWDTNVAYS
                     NTLEQGNLAAAFRDAPVIVRHRMLNQRLAPVSLEPRGTLAMYDGSLGQGLLTVWTSTQ
                     EAHAIRDGLSGVLGLEATRIRVVTPDVGGGFGSKLNTYPEDAVVAHLARRLRRPVKWV
                     ETRQENLLTTTHGRGQVIEVEVAAERDGRIRGMRSRVLADLGAHLVYLTAIVPTLTPL
                     MIQGPYDIPALRCELGALYTNTCPTGAYRGAGRPEATYYLERVMDLVADETGVDPAEV
                     RRRNFIPPTRFPYRAASGVQYDSGDYTKPLDQVLRLTGYAQLRAEQEARRRDGALMGI
                     GLSSYVEICGFGPWELGTVRVNKDGTATVVTGTSPHGQGDATGFAQLVADALTIPIEQ
                     ISVVSGDTLLVQYGGGTSGSRSMSLGGSAVYLAAQEVRGQILGIAADLLEAAPADLAL
                     ANARVAVRGAPGKEVSIAEVAEAAYNGSHLPEGQAPGLEATSRFKSEGTTFPFGSHLC
                     VVEIDPETGGVRIVRYVAVDDCGRVINPLLVDGQVHGGIVQGASQALLEAVVYDDQGQ
                     LLTRTMSEYGIPNAHLLPRMERGTSVTPTPRNPLGAKGI"
BASE COUNT          803 a         1994 c         2019 g          815 t
ORIGIN      
        1 cacggtgctc aatggcgtgt atcaacggga ctatccggtc gtccaggggg cggtgatgct
       61 gttcgcggcc acgttcttgg tgacgaacct gctggtcgac ctcgcgtaca cgtatctcga
      121 tccacggatt cgttatgagt aggaccggcg tgggcgttca ggatgcgggc ggggggatcg
      181 gcccgcccgg caccgcgcgg cgggccgaag acccgctccg cctggcgcgc cggaacccgg
      241 tggcggcgat tggggcgggg ctgattgcga tcgtggtcct cctggcgctc ggggcgaacc
      301 tgatcgcgcc ctacggcccg gtcgcccaga tcgccccgcc gctcctgcgg ccgggccacg
      361 ggttcttcgc cgggaccgat gaattggggc gcgacgaact cagccgcctc atctttgggg
      421 cgcgcgtctc gctgtacgtc ggggtgctgg cggtctccat cgcgctggcg ggcggcgcga
      481 cctcgggcat catcgccggg ttctacggcg gctggctgga caacgcgttg atgcgcacca
      541 tggacatcct gttctcgctg ccggcgatcg tcctcgcgat cgcgatcacg agcattcttg
      601 ggccgagtct cacgaacgcg atgatcgcga tcggcatcgt gtatgcgccg acgttcgccc
      661 gcatcgcgcg cgggccgacc ctcgcggtga tgaacctggc gtacatcgag gcggcgcgcg
      721 ccatcggggc gccgaactgg acgatcatcc tgcgccatgt cctgccgaat gtgtcggccc
      781 cgctcctggt gcagacgacc gtgtccctgt cgacggccat cctcaccgag gccgcgttga
      841 gcttcctggg cctcgggacc cagcccccca ccgcctcctg ggggttgatg ctcaacgccg
      901 cccggcagta catgctgatc gacccgtgga tcgcgatcct gcccggggtg gcgatcgccc
      961 tgaccgtgct cggcttcaac ctgctggggg acggcctgcg cgacctcctc gacccgcgca
     1021 tccggacgat gtgaccggag ccggttacag cacgccggcc agcgctttcc ccagccgccg
     1081 gttgagcgcg ctcaggatcg cacgcgagac cgcggtgtgg gggtcgggag cgccggcgct
     1141 gccgaccagg cgctcggagg gatccgcggc gctgatcagc accgccacgg ccaccgtgtc
     1201 ctgctcggac ggcgtcagcg tgaggtcgtc gaggagatac cccgggctct cgccgaccgc
     1261 ctgggtgatc gcccggagcg tcgcctccgc gacgaggaac ggcgcgccat gcccgaggat
     1321 ccgcgcagag gcggtgcccc gcaccgcccg gccgcggagg ctcaactcca ccaccgcggt
     1381 gctcccctgg acgtcccggt tgacgctcgc ccgcaccagc cgcagccgca cctcgtccga
     1441 ccgctggtcg gctccgccgt cacgcggggc ggcgtcctct tcccgccggc tgtagtggac
     1501 gatgccccgc cccgactcct tcgccagggt ggaaccccgg ctcgcgagcg tcagaaggtc
     1561 ctccacggtc gacgcgatgt gcgcggccgt gcgcggctcc acccggcggc ctccggcaaa
     1621 cccgatcgcc accgtgaccc gctcctcgcg cccctggatc tccaccggca ggttgaccgc
     1681 gtcccggagc cgctgggcga ggcctcgggc gtcctcgctc cccctcgtcg tcgccaccgc
     1741 gaactcgtcc cccgcgtacc ggcagaggag gtcggtggcg ggatcgatct ggctggccag
     1801 gagatacgcc accgaccgga tgatatcgtc gccgacgacg tgccccaacg ccttgttgat
     1861 gatcctgaag ttgtccaggt cgatgaacag caccgcgacc tcccggcctc cctcgagcgc
     1921 gctcgtcgcc caggtgcgca gcgcagtcgc ccaggggagc ccggtcaagg gatcccgctc
     1981 ctggctgcgg gcctcgagga cggcggcgag ggtgatcagg ccgacgagcc gtcctccctc
     2041 gaccacgggc aggacgtccg cgcgctgtcg catgaggagc tcgtaggcct gcccgatcga
     2101 gaggtcgggg cgggcggggg agaggtccgt ggccatgacc tcggccacgg tccggtacag
     2161 gggttggcgg agcaggtgga tcggggtgac aaaccccacc aaccgggctc cgtcgaccac
     2221 gggcagcccg ccgtgatcat gcgcgcgaat taggtcgatc gcctcgccga cggtggccgc
     2281 ccccgggatc ggggtgggga ccgggatcat caaatccgcc accgtgctcg gagattctct
     2341 catgccttcg gacgcccttg cacggggcgg cgtgcgctca tcctcaagcg tatcatcggg
     2401 tttcggaaga gcgggcgcca ccccccaaaa gaatggactt tcccatcgaa cacgcgggcc
     2461 ccgcgcgggt cttggggtag agggttctgg gcatgtacac gggtatagac tattcgacgc
     2521 gcgggccgtt cccaccggtc gaactactgc gaagggacga ctaccatagt ccgtccgacc
     2581 caaactgccg gcagcagcac aagccaaagg tcgctgatcg agatgggcaa gacccacgag
     2641 gcggggctca agatggccga gcggcccaag cccccgggcg cccgatcccg gcgcacggga
     2701 ggcaggcggc tccgagagct tcgtgcagcg gcgggggcgg acgagcggcg ccggctcgtc
     2761 caggccctcc acgatggtcc cctgcagatg gccaccgcgg ccaagatccg gctccaggcg
     2821 cgccggcagg gtgtcgggga tcgcacggcg gccaagagcc tggatgaggc gatcgcgctc
     2881 atcggacagg ccatcgccgc gatgcgggcc ctgctgcgcg acccagcgcc gccttcgggg
     2941 tccgggtctc tccaggtccg cctccggcga gcggccggac gctggggcga ggtgaccggc
     3001 gtgcgcgtgc acctgagcgt cctcggcgct gcccgtgctt cggcattctc cagggaaacg
     3061 ctggaggtcg ccgagcacgt cgtcagcgag agcatcgtca acgcatggaa gcatgggagg
     3121 gcgacccggg tgtcggtcag gtgcgagcgc caccagggcg ggctcctcct gaccctgcag
     3181 gatgacggac gcggattccg gccatcgatc gtgcccgagc cgcggaacgg cacgaggatg
     3241 gggctccggc tcctccgttc gcgggtggac gagctcgggg ggtggttcga cgtccgttca
     3301 tcgcacggcg gcggcgccgt cgtccaggcc tggcttcccc cgcgccccgc gtcccaccga
     3361 gcgcccgagt agcgcggtcc ctgccctgtg ccgccccgaa cgcaggggcg ccctcgtccc
     3421 cggtcgaaca cgtctgtatc cacgcatttg acggggcggt gtgatcccat ggcggtgtcg
     3481 cagtatgtgg gcgcccgggt caagcggatc gaggatccca agctgatccg gggcgagggg
     3541 gcgtacctcg acgacctccg cctcgcgggc atgctgcacg ccgtgttcat ccgcagcccg
     3601 cacgggcacg cccggatcgg cgggatcaac ctgtggcgcg ctcgcgccca tccgggggtc
     3661 gcgggcgtcc tggctggcga cgacctgcgc gagatccagc gcccgctccc ggtcatgccc
     3721 gtcgagggga tgcgagcggc ggatcacctg cccctggcct cgggcgaagt gtgctacgcc
     3781 ggcgagccgg tggcggtcgt cgtggcggag gacccgtaca tcgcccgcga tgcggccgac
     3841 ctcgtcgaga tcgcgtacga cccgctgccc gcagcgatcg atctggagcg ggccgccaag
     3901 ggggcgccgt tcgcgcacgc cgggtgggac acgaacgtcg cgtactccaa taccctcgag
     3961 cagggcaacc tggccgcggc cttccgggat gcgcccgtga ttgtccggca ccggatgctc
     4021 aaccagcgcc tcgcccccgt gtcgttggag ccccggggga cgctggcgat gtacgacggc
     4081 agcctcgggc aggggctgct gaccgtgtgg acctccaccc aggaggcgca tgcgatccga
     4141 gacggcctca gcggggtcct cgggctggag gccacccgca tccgggtggt gacgcccgat
     4201 gtcgggggcg ggttcggctc caagctgaac acctaccccg aagacgcggt cgtcgcccac
     4261 ctggcccgcc ggcttcgccg gcccgtgaag tgggtggaaa cgcgccagga aaacctcctg
     4321 acgacgaccc atggacgcgg ccaggtcatc gaggtggagg tcgcggccga acgggatggc
     4381 cggatccggg ggatgcgcag ccgggtcctc gccgacctcg gcgcccacct cgtctatctg
     4441 accgcgatcg tccccacgct gaccccgctg atgatccagg gcccgtacga catcccggcc
     4501 ctccgctgtg aactgggcgc gctctacacg aacacctgcc caaccggcgc ctaccgcggc
     4561 gcgggccgcc cggaagccac gtactacctc gagcgggtca tggacctcgt cgccgacgaa
     4621 accggggtgg acccggccga ggtgcgccgc cgcaacttca tcccgccgac ccggtttccg
     4681 tatagggcgg cgagcggcgt gcagtacgac tcgggcgatt acacgaagcc gctggaccag
     4741 gtcctccggc tgaccggcta cgcgcagctc cgcgccgagc aggaggcgcg gcggcgcgac
     4801 ggagcgctga tgggcatcgg gctcagcagt tacgtggaga tctgcgggtt cggcccctgg
     4861 gaactcggga cggtgcgcgt caacaaggac gggaccgcca ccgtcgtcac gggcacctcg
     4921 ccccacggcc agggggacgc caccgggttc gctcagctcg tcgccgacgc cctgacgatc
     4981 ccgatcgagc agatctcggt cgtgtccggg gacacgctcc tcgtgcagta cggcggcggc
     5041 acgagcggca gccgcagcat gtccctcgga gggtcggcgg tgtatcttgc ggcgcaggaa
     5101 gtgcgggggc agatcctggg gatcgccgcg gacctcctcg aggccgctcc ggcggatctg
     5161 gcgcttgcca acgcccgcgt ggccgtgcgg ggagcgccgg ggaaagaggt ctcgatcgcg
     5221 gaggtggccg aggcggcgta caacggctcc catctgccgg agggccaggc cccgggattg
     5281 gaggccacca gccggttcaa atcggagggc acgacgtttc cgttcgggag ccacctctgc
     5341 gtggtggaga tcgaccccga gaccgggggc gtgcgcatcg tccggtacgt ggccgtggat
     5401 gactgcgggc gcgtgatcaa cccgctgctg gtggacggac aggtccacgg agggatcgtc
     5461 cagggggcga gccaggcgct gctcgaggcg gtggtgtacg acgatcaggg gcagctgctc
     5521 acccggacga tgtcggagta cgggatcccc aacgcccacc tgctcccccg gatggagcgg
     5581 gggacctcgg tgacccccac cccgcggaac ccgctcgggg ccaaggggat c
//
LOCUS       DASQGH010000278         8740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38150, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000278 DASQGH010000000
VERSION     DASQGH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38150"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..636)
                     /locus_tag="VEW91_05435"
     CDS             complement(121..636)
                     /locus_tag="VEW91_05435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYM91060.1"
                     /translation="MKAQELRDIQAPLKARYRDAPNSAVITLKARGRLGSESITCKVE
                     TGRAIVEAGLHPATGGDGLSLCSGDMLLEALVACAGVTLKTVATALDLPLKGGTVSAE
                     GDLDVRGTLGVDREAPVGFREIRLRFDIDCPASPDQIATLLKLTERYCVVFQTLNKRP
                     HLAVSHHLTGS"
     gene            complement(700..1812)
                     /gene="ald"
                     /locus_tag="VEW91_05440"
     CDS             complement(700..1812)
                     /gene="ald"
                     /locus_tag="VEW91_05440"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYM91061.1"
                     /translation="MRVGVPKEIKDQEGRVGITPAGVHELVDAGHTVVIQTGAGEGSG
                     ISDRDYERRGARIARSAAEAWDAEMVMKVKEPLLEEYAYLRPRQILFAYVHLAAAPEL
                     TQTLLESGTVTIAYETVQLPDGELPLLTPMSEVAGRMAVQEGAFYLKETAGGKGTLIG
                     GVTGVPPANVVIIGAGVVGTHAAKVATGMGAQVTVLERNPRRMAYLEDILHGRVMMVM
                     SNPVTLEQAVAYADLLVGAVLIPGARAPRLVTEAMVRTMKPGSVIIDVAVDQGGCVET
                     TEPTTHSHPIVVKHDVLHYGVTNMPGALPRTSTYALTNATIRYALEIAGKGWRRAASD
                     DPALARGVNTAEGYLTHPAVAAAHGLPYAPLEKVLR"
     gene            complement(2073..3470)
                     /locus_tag="VEW91_05445"
     CDS             complement(2073..3470)
                     /locus_tag="VEW91_05445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYM91062.1"
                     /translation="MPQTAGITPLTFARTHIQEFERDLCEWLRIPSISMLPAYRSDVE
                     RAARWVVDHLRAIGMDSRLVSGKGHPLAYGEWLGAPGRPTVLAYGHYDVQPADPLDLW
                     TAPPFEPSRRGGSLYARGAADNKGPIFTFLKAVECYLAIGTPLPVNVKVLVEGEEECG
                     GGVVASFLRSDPERVHADAALVLDSGMFAPGVPAITLGLRGIVSAEVEVSGPGRDLHS
                     GLYGGVTINPFLVLAEILTGLIDPQGRILIPHFYDRVAPPEPEERAAWSRLPFDEADS
                     VQNEVGAPLVGEPGFTVLERLWARPTLDVHGMPGGFTGEGIKTVIPARAAAKISMRLV
                     PHQEPEEIYELFEAHVRRLAPPGARVGVRPLGSAPPVLIDPRAPAVEAARAALTETFD
                     REAVLIRSGATVPIVADLATQLRIPTVVTGWSLPDAGWHSPNEKLDLNHFRLGIEALI
                     RFLARFGALNVTHSS"
     gene            3525..3601
                     /locus_tag="VEW91_05450"
     tRNA            3525..3601
                     /locus_tag="VEW91_05450"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3559..3561,aa:Met,seq:cat)
     gene            3685..5154
                     /gene="tilS"
                     /locus_tag="VEW91_05455"
     CDS             3685..5154
                     /gene="tilS"
                     /locus_tag="VEW91_05455"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HYM91063.1"
                     /translation="MSATDTMAKAAGEAGRGKTGPPLVDRIRDTLRRHRMLAGGETVV
                     VGASGGPDSTALAHGLATLGPELALTIHLAHLNHQLRPDAAEDAAFVAALARALGLAH
                     HQDSADPRALADREGLSLEDASRRLRYEFLTRVAHDAGAAVIATGHTLDDQAETVLMR
                     LLRGSGLVGLTGIPPVRESGTIRIIRPLIDTTRGEVQAYLQSVGADWREDATNRDLTI
                     LRNRVRLVLLPMLEGYNPEVRQALARVAALLRDEAEAIDALAASPIAGVLSGGPPVVQ
                     VAVGPFARLPVALQRRALRGAVERARGNLRATRFVHIEEARRLVLEGQVGSWLSLPGG
                     VRITRLADSVEVSAVEPARPPGAYRLPVPGRVVALEFGVHLTAEAIPRDRLAAGPETP
                     RPDEVVLDAMQVGGELILRGPQPGDRFAPAGMRGRTKLVAEYLSDEKVPRHRRPFVPV
                     LATREGAILWIVGMRAAETARVTPATAKAVRVVARRLRA"
     gene            5259..7088
                     /gene="ftsH"
                     /locus_tag="VEW91_05460"
     CDS             5259..7088
                     /gene="ftsH"
                     /locus_tag="VEW91_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYM91064.1"
                     /translation="MFNKYVRNLLVWALILVVVLYLVLPLYNNRETPREVIAYSEFIS
                     NAQQGHIDQVTIGDEAVSGQLKSGKAFRTYIPKGDASYLEILRTHGATIRVEAPSRSA
                     LWPNLLTTMLPFLVLVGLWMLMLRQAQSGSNQAMSFGKSRARLHTEAKTRVTFDDVAG
                     VDEAKEELQEIIEFLRHPKKFQALGAKIPRGVLLVGPPGSGKTLLAKAIAGEAGVPFF
                     SISRSEFVEMFVGVGASRVRDLFDQAKKSAPCLVFIDEIDAVGRQRGAGGGGGHDERE
                     QTLNQLLVEMDGFDPNSGIIVIAATNRPDILDPALLRPGRFDRRIVVDNPDTKGRRAI
                     LDVHGRGKPIAEDANLDVLAKRTPGFSGADLANTVNEAALLAARRGKKRIGMAEFDEA
                     IERVIAGPQRKSRILSPKERELTAYHEGGHALLGKLVPHANPPHKVTILPRGMALGYT
                     IPLPPEDRYTVTRSEILANITAILGGRVAEEIVFGEITTGAANDFEKATELARKMVTE
                     FGMSDKLGPLTLGTKHGPVFLGRDLVESRNYSDEIAYEIDKEVRRIIDECYGRARTVL
                     TDHREILDRIAKALLERETLNGEQLDALVAGRPLPGFAPAVAA"
     gene            complement(7315..7569)
                     /gene="rpoZ"
                     /locus_tag="VEW91_05465"
     CDS             complement(7315..7569)
                     /gene="rpoZ"
                     /locus_tag="VEW91_05465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYM91065.1"
                     /translation="MRQPAVEELVARTRNRYLAVAIVMLRARQLIAGDMPVIDTAARS
                     PVTVALEELAQGRLRAEIREGHSRLGREDVFGAAGREEAA"
     gene            7849..8061
                     /locus_tag="VEW91_05470"
     CDS             7849..8061
                     /locus_tag="VEW91_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYM91066.1"
                     /translation="MTRVKGTVKWFNESRGFGFIAVEGGGKDVFVHHSAIKGPGRPSL
                     REGQQVEFEIVQEARGGRAVNVTPLD"
     gene            complement(8182..>8740)
                     /locus_tag="VEW91_05475"
     CDS             complement(8182..>8740)
                     /locus_tag="VEW91_05475"
                     /inference="COORDINATES: protein motif:HMM:NF010248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HYM91067.1"
                     /translation="KNLLLTGRPGVGKTTIVRKVVEQLGPRTCGFFTEEIREESRRTG
                     FKIITTDGREAILAHIHVRSPHRIGRYGVSAEGMRQLAIPTLLLALKNAGAIVIDEIG
                     RMELISPEFEGAVLECLDSPRPVVAVIQMVDLPFLNRIRARPDVEVIEVTLPNRDALP
                     AYVMTRLQELLKARGGPSKAKRPFS"
BASE COUNT         1419 a         2875 c         3020 g         1426 t
ORIGIN      
        1 atcgccgccg gcaagatccg gatcgacagg tcgaaagaac cggcaacgga gtagcaccag
       61 gggtcccgcg ggcgcatttg gccgcggggc ctttttgttg gtgggggggc cgcggctgaa
      121 tcagctgccg gtgaggtggt ggctgaccgc gaggtgcggg cgcttgttga gcgtctggaa
      181 aacgacgcaa tagcgctcgg tgagcttcag cagcgtcgcg atctggtcgg ggctggccgg
      241 gcagtcgatg tcgaagcgca gccggatctc acggaacccg accggtgctt cgcggtcgac
      301 gccgagcgtg ccgcggacgt cgagatcgcc ctcggcgctc accgtgccgc ccttgagcgg
      361 caagtcgagc gcggtcgcga ccgtcttgag cgtgaccccg gcgcaggcaa ccagcgcctc
      421 caagagcatg tcgcccgagc acagactcag gccgtcgcct cccgtcgccg ggtgaagccc
      481 ggcctcaacg atggcgcggc ccgtctcgac cttgcaggtg atgctctcgc tcccgagcct
      541 gcccctggcc ttgagcgtga tcaccgcgga gttcggcgcg tcgcggtagc gcgctttgag
      601 cggcgcctgg atatcccgca gctcttgagc cttcatcttc cctgctccgt gtatctcgcc
      661 acccctggcg tcattggcga atcgcgctag cgggcgtccc taccgcagga ccttctcgag
      721 aggagcatag gggagcccgt gcgcggcggc gacggccggg tgggtcaggt acccctcggc
      781 cgtgttgacg ccccgcgcta acgccgggtc gtcgctcgca gcgcgccgcc atcctttccc
      841 ggcaatctcg agcgcgtacc ggatggtggc gtttgtcagc gcgtacgtcg acgtcctggg
      901 cagcgcgccc ggcatgttgg tcaccccgta atgcaggacg tcgtgcttca ccacgatcgg
      961 gtggctgtgc gtggtgggct cggtcgtttc gacgcatccg ccctgatcga cggcaacgtc
     1021 gatgatcacc gaccccggct tcatggtgcg taccatcgct tcggtcacga gccgtggggc
     1081 gcgcgctccg gggatgagga cggcccccac caggagatcg gcgtaggcga cggcctgttc
     1141 cagcgtgacg gggttgctca tcaccatcat cacccggccg tggaggatgt cttccaggta
     1201 ggccatgcgg cggggattcc gctcgagcac ggtgacctgg gcgcccatcc cggtggcgac
     1261 cttcgccgcg tgcgtcccaa caacccccgc gccgatgatg acgacgttgg ccggcgggac
     1321 cccggtcacg cccccgatga gcgtgccttt gccccccgcc gtttctttca agtagaacgc
     1381 gccctcctgg acggccatcc ggccggcgac ctcgctcatc ggggtgagga gcggcagttc
     1441 gccgtcagga agctggaccg tctcgtacgc gatggtgacg gtgccggact ccaggagggt
     1501 ctgggtgagc tcgggtgcgg ccgccaggtg gacgtacgca aacaggatct ggcggggtcg
     1561 gagatacgca tactcctcga ggagcggttc tttgaccttc atcaccatct cagcgtccca
     1621 ggcctccgcg gcagagcgcg cgatgcgcgc cccccgtcgc tcgtagtccc ggtccgagat
     1681 cccgctgcct tcgcccgctc cggtttggat gacgaccgtg tggccggcgt ccacgagctc
     1741 gtggacaccg gccggcgtga tgccgacgcg gccctcctgg tccttgattt ctttcggaac
     1801 cccaacgcgc acggaataga tgctcccttc gcgtgcctgg ctcagtgttc cacgatgcga
     1861 tgtggtcctc ctctgatttc cacaagatat ccccacaccg atccacagct caaggtcaaa
     1921 cgatcgttcc ggattggcgc ccacatcaaa gtgtgctaga gtgtccgttg ccagcgagcg
     1981 ccacatcagg atgagagaga ggtagctctt cggagccaac ccggcttaac ctgtgtgtgg
     2041 tctcgctggt atattgttga gcgggtgcgg gatcagctgc tgtgcgtcac gttcagcgcg
     2101 ccgaaccgcg ccaggaaccg aatgagcgct tcgatgccca gacgaaagtg gttaaggtcc
     2161 agcttctcat tcggactgtg ccagcccgca tcgggaaggg accatcccgt caccaccgtt
     2221 gggatccgaa gttgggtggc caggtcggcg acgatcggta cggtggcgcc ggaacggatc
     2281 aggaccgctt cgcggtcgaa cgtttcggtg agcgcggctc ttgccgcctc gaccgccggt
     2341 gcgcgggggt cgatgagcac cgggggcgcg ctgcccaggg ggcgtacacc gacccgcgcg
     2401 ccgggagggg ctaagcggcg cacgtgcgcc tcaaacagct catagatctc ctccggttcc
     2461 tggtgaggca caagccgcat gctgatcttc gcagcggcgc gggccgggat gaccgtcttg
     2521 atgccctcac cggtgaaccc tcccggcatc ccatggacgt cgagcgtcgg ccgggcccat
     2581 agccgttcca ggacggtgaa ccccggttcc cccaccagcg gagcacccac ctcgttctgc
     2641 acggagtccg cttcatcgaa cggcaggcgg ctccaggccg cccgctcctc gggctcaggc
     2701 ggcgcgaccc gatcgtagaa atgcgggatg aggatccggc cctggggatc gatgaggccg
     2761 gtgaggatct cggccaggac gagaaacggg ttgatcgtta cccctccgta gagcccggag
     2821 tggagatccc ggccggggcc ggagacttcg acctcagcgc tcacgatgcc gcggagcccg
     2881 agggtgatgg cgggaacccc aggggcaaac atcccggagt ctagaacgag cgccgcatcc
     2941 gcgtgcacgc gctcggggtc ggaccggagg aacgacgcga ccaccccgcc accgcactcc
     3001 tcttcccctt cgaccaggac cttcacgttg accggcagcg gggtcccgat ggcgaggtaa
     3061 cactccaccg ccttgagaaa cgtgaagatc ggccctttgt tgtcggccgc cccgcgggcg
     3121 tagaggcttc caccgcgccg cgacggctca aacggcggtg cggtccacag gtcgaggggg
     3181 tccgcaggct gcacatcgta gtggccgtag gcaagcacgg tgggccgtcc aggggccccg
     3241 agccactcac catacgcaag cgggtgcccc ttgcccgaca ctaggcggct gtccatcccg
     3301 atggcgcgaa gatggtccac cacccagcgg gcggcccgtt cgacgtcgga tcggtaggcc
     3361 ggcagcatgc tgatgctcgg gatgcgaagc cactcgcaca ggtcgcgttc gaactcctgg
     3421 atgtgcgtgc gggcgaacgt gagcggggtg atccccgcgg tctggggcac gccggagggt
     3481 tctccctgac atcacccgct cctgcctgct ataatggacc atccggcggc gtagctcagg
     3541 tggccagagc acgcggttca tacccgcgga gtcgggggtt cgaagccctc cgccgccacc
     3601 actctcgagc tgtgtgaatc ctcccccagg accacgcgcc ggcaccgccg aatgtgcccg
     3661 cgggatccat gggcatgggt gtgtgtgagc gcgacagaca cgatggcgaa ggcggccggt
     3721 gaggcggggc ggggaaaaac gggcccgccg ctggtcgacc gcatccggga caccctccgt
     3781 cggcatcgca tgctcgcggg cggggagacc gtggtggtcg gggcctccgg ggggcccgac
     3841 tcgaccgcgc tcgcccacgg cctcgccacc ctcgggcccg agttggccct gacgattcat
     3901 ctggctcacc tcaaccacca actgcgcccg gacgccgcag aagacgccgc gttcgtcgcc
     3961 gcgctggctc gcgccttggg cctggcgcac caccaggact cggccgaccc gcgagcgctg
     4021 gctgatcgcg agggtctctc gctcgaggac gcgtcccgcc ggcttcgcta cgagtttctc
     4081 acccgcgtgg cgcacgacgc cggggccgcg gtgatcgcca ccgggcacac gctggacgac
     4141 caggccgaga ccgtgctgat gcgtttgctt cggggcagcg ggctggtcgg gctcacgggg
     4201 atcccgccgg tgcgggagag cggcaccatc cgcatcattc gtccgctcat cgacacgacc
     4261 cggggggaag tccaggcgta cctccagtcg gtcggggccg attggcgcga agacgccaca
     4321 aaccgggatc tgacaatcct ccgcaatcgc gttcggttgg tcctcctgcc aatgctcgag
     4381 gggtataatc cagaagtgag gcaggcgctg gcccgggtgg ccgccctgct gcgagacgag
     4441 gccgaggcga tcgacgcgct cgcggcctcc cccatcgcgg gggtgctgtc cggcggtcca
     4501 ccggtggtgc aggtggcggt cgggccgttc gcccgtctgc cggtggcgct ccagcggcgc
     4561 gcgctcagag gggctgtcga gcgcgcgcgc gggaacttgc gcgcgacccg gttcgttcac
     4621 atagaggagg cacgccggct ggtgctcgag gggcaggtgg gctcctggct gtcgctcccg
     4681 ggaggcgtgc gcatcacgcg gcttgcggac agcgtcgagg tgagtgcggt cgagcccgcc
     4741 cgacctccgg gggcgtaccg gttgccggtg ccgggccgag ttgtggccct cgagttcggc
     4801 gtgcatctga ccgccgaagc gattcctcgg gatcgactcg cggccggccc ggaaacgccc
     4861 cggccggacg aggtggtgtt ggacgcgatg caggtcggag gggaactcat cctgcggggc
     4921 ccgcaaccgg gggaccggtt cgcgccggcg ggaatgcgtg ggcggacgaa gctggtggcg
     4981 gagtacctga gtgacgagaa ggtgccgcgg caccgccgtc ccttcgtgcc ggtgctggcg
     5041 acgcgcgagg gagcgatcct gtggatcgtc ggcatgcggg ccgcggagac cgcgcgggtg
     5101 acgcccgcga ccgcgaaggc ggtgcgggtc gtggcgcggc ggctgcgggc gtaacatgtg
     5161 ctataattcg ttgatagggc cgatccttcg ggacgccgcg ggcttcgacg ggccatgcgc
     5221 gcggttcgat gatcggccgg ctctaggggg tcgcttgcgt gtttaacaag tacgttcgta
     5281 acctgctcgt ctgggcgctc atcctcgtcg tcgtgctcta tctggtgctc ccgctctaca
     5341 ataaccggga gacgccgcgc gaggtcatcg cctacagcga gtttatctcc aacgcccagc
     5401 agggccacat cgaccaggtc accatcggcg atgaggccgt gtccggccag ttgaagagcg
     5461 gcaaggcgtt tcgcacgtac atccccaagg gcgatgcctc gtatctggag atcctgcgca
     5521 cgcacggcgc cacgatccgc gtcgaggctc cgtcccgctc agcgctctgg cccaacctgc
     5581 tgaccacgat gctgcccttc ctggtcctcg tgggcctgtg gatgctgatg ctgcggcagg
     5641 cgcagtcggg gtcgaaccag gcgatgtcct tcgggaagag ccgcgcccgg ctgcacaccg
     5701 aagcgaagac ccgggtgacg ttcgacgacg tcgcgggagt ggatgaggcg aaggaggagc
     5761 tccaggagat catcgagttt ctccgccacc cgaagaagtt ccaggcgctg ggggccaaga
     5821 tcccccgagg ggtgctgctt gtggggcccc cggggagcgg caagacgctc ctggccaagg
     5881 cgatcgccgg cgaagccggg gtgccgttct tctcgatctc caggagcgag ttcgtggaga
     5941 tgttcgtcgg ggtcggcgcc agccgggtcc gcgacctgtt cgatcaggcg aagaagagcg
     6001 cgccgtgcct ggtgttcatc gacgagatcg acgcggtggg ccggcagcgg ggcgcggggg
     6061 gggggggcgg ccacgacgaa cgggaacaga cgctcaacca gctcctggtg gagatggatg
     6121 ggtttgaccc caacagcggg atcatcgtta tcgcggcgac caaccggccg gacatcctgg
     6181 acccggccct cttgcggccc gggcggttcg accgccggat cgtggtggac aacccggaca
     6241 ccaagggacg tagagccatc ctggacgtgc acgggcgggg gaagcccatc gccgaggacg
     6301 ccaatttgga cgtgctggcc aagcgcacgc cggggttcag cggggcggat ctggcgaaca
     6361 cggtgaacga ggcggcgctc ctggcggcgc ggcgggggaa gaagcggatc ggcatggccg
     6421 agttcgacga ggcgatcgag cgggtcatcg ccgggccgca gcgcaagagc cggatcctgt
     6481 cccccaagga gcgggaactg accgcctacc acgagggcgg gcacgcgctg ttgggcaagc
     6541 tcgtgcccca cgcgaatccg ccgcacaagg tcacaatcct cccgcggggc atggccctcg
     6601 gctacacgat cccgctcccg ccggaggaca ggtacaccgt gacgcgcagc gagatcctgg
     6661 cgaacatcac ggccatcctc gggggccgcg tggccgagga gatcgtgttc ggggagatca
     6721 ccaccggcgc ggcgaacgac ttcgagaagg ccaccgagct cgcccgcaag atggtgaccg
     6781 agttcgggat gtccgacaag ctcgggccgc tcacgctcgg gaccaagcac gggccggtgt
     6841 tcctgggccg ggacctggtg gagagccgga actactcgga cgagatcgcc tacgagatcg
     6901 acaaggaagt ccgccggatc atcgacgagt gctacggccg ggcccggacg gtcctcaccg
     6961 atcaccggga gatcctcgac cgcatcgcca aggcgctctt ggagcgggag acactgaatg
     7021 gtgagcaact cgacgcgctc gtcgctggcc gaccgctgcc cggcttcgcg cccgcggtcg
     7081 ccgcgtgaag gcgcatgtcg tcacggcgag ggggagagta tcatggccga ggtccggggg
     7141 gaacctatgg cacagtggga tttgtggccg gcacaggcga ctgcagttgt aggggaaggg
     7201 gttcgcccgc cagccgcgag cggccgggat gggcgcacag gcgttcggtg acagcatcac
     7261 cgggtgtgct cggcgaacgc gccctgcacg cgccgcctac tgctcgcgcc caggttaggc
     7321 agcctcctcc cggccggccg ccccaaacac gtcctcccgg cccagccggc tgtgcccctc
     7381 acggatctcg gcccgcagcc tgccttgggc cagttcctcc aacgcgaccg tgaccgggct
     7441 tcgggcggcg gtatcgatca caggcatatc accggcgatc agttgccgtg ctcgcagcat
     7501 cacgattgcc actgccaagt accggtttct cgtccgagcc acgagctcct cgaccgcagg
     7561 ctgccgcatc caaaccacct tctcctggga aaaccagtct tccacctttt aatgtaccga
     7621 gccctccgca ccggtcctat cgggcccacg gtgtatcttc gaacccgtgt gttctgggtg
     7681 gccccaagaa tacgacccgg tatcggcctc agcgaagagg gcggtccaac gaccatcgct
     7741 ccgtccatac ggtacacgcc cgatggcgtt cgagacacgt cacggatatc ctgaaggtgc
     7801 aggggggagt ggtgtactta gaaggaggca atcacgatgg cagggccggt gacaagagtc
     7861 aaaggaaccg tcaagtggtt caacgagagc agagggtttg gcttcattgc ggtggaaggc
     7921 ggcggcaagg atgtgtttgt gcaccattcc gcgatcaaag gcccgggccg ccccagcttg
     7981 cgtgaaggcc aacaggtaga gttcgagatc gtccaggagg cgaggggtgg acgggcggta
     8041 aacgtcacgc cccttgattg agcccagagc cacttgctga tgacctccgg cgggccccgc
     8101 atacagtcgt acccgagcca cgcccgtccg tatttttatc ggctttcgat caaagccgct
     8161 gatccgaggc cgtagcgaca gctacgagaa cggacgcttc gccttcgatg ggccgccgcg
     8221 ggctttcagc aactcctgca atctggtcat gacgtaagcc gggagggcat cgcggtttgg
     8281 aagggtgacc tcaatcacct cgacatcagg gcgggcccga atccggttca agaatgggag
     8341 gtcgaccatc tggattactg ctaccaccgg ccgggggcta tccaggcatt cgaggacggc
     8401 accttcgaac tctggagata tcaactccat gcgcccgatc tcgtcaatca caatcgcgcc
     8461 cgcgttcttc agcgccagta gcagagtggg gatcgcaagc tgacgcatgc cttctgcgct
     8521 gacaccgtac cggccaatgc gatgaggcga acgcacgtga atgtgcgcca ggatggcctc
     8581 ccggccgtcc gtggtgatga tcttgaagcc ggtgcgccgg ctctcctctc ggatttcttc
     8641 agtgaagaat ccacacgttc tcggacccaa ttgctcgacg accttccgca cgattgtcgt
     8701 cttgccaact cccggccgcc cggtgagcaa aaggttcttt
//
LOCUS       DASQGH010000279         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1218, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000279 DASQGH010000000
VERSION     DASQGH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /gene="metG"
                     /locus_tag="VEW91_05480"
     CDS             complement(<1..1522)
                     /gene="metG"
                     /locus_tag="VEW91_05480"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYM91068.1"
                     /translation="MEPFYVTTPIYYDNDVPHVGHAYATLAADTIARWKRLAGAEVFF
                     LTGTDEHGVNIERRAREAGVTPQVWCDRIAPQWQALWKRLGITNDDFIRTTEPRHIRL
                     AQHLFQQAYDRGAIYKGTYEGWYCPSCEAFYTEDELLEGTLCPIHKRPVEWTAEENYV
                     FRLSQYQPWILRKIEEEPGFIQPETQRNEMLSLVRGGLRDLAVSRLSVKWGIPVPFDP
                     AQSIYVWIEALMNYVTAIGYLDDPARYRRFWPAAHHLVGRDINRFHSVIWPCFLEAVG
                     LPSPKQVWAHGFWTVEGEKMSKTRGNFIDPIREITRLAEASGAEWEVAADAFRYMLLR
                     EVPFGQDGDYSHAALIHRFNADLANDFGNLLNRTLPLVERHFGGRIPSPGSPEGPDGA
                     LRAAAADLPAAADRRIGTFDFTRGLAEIWRFLGGANKYIDEAAPWSALKTGNAARAGA
                     ILYNTLEALRIAPVLLAPVLPAAAQRVWERLGIPTPLASQRLTDARWGGLVAGIPTR"
BASE COUNT          310 a          750 c          677 g          324 t
ORIGIN      
        1 gccgggtcgg gatccccgcc acgagcccac cccaccgggc gtctgtgagc cgctgcgagg
       61 cgagcggggt ggggatcccg agccgctccc acacccgctg cgccgccgcc ggcagcacgg
      121 gggcgagcag gaccggggcg atccggagcg cctcgagcgt attgtacagg atggcccccg
      181 cgcgcgcggc gtttcccgtt ttgagcgccg accagggcgc cgcctcgtcg atgtacttgt
      241 tcgccccccc gaggaagcgc cagatctcgg ccagcccccg ggtgaagtcg aacgtcccga
      301 tgcgccggtc tgcggccgcg gggaggtccg ccgccgccgc ccgcaaggca ccgtccggcc
      361 cttcggggga gcccggggag gggatccggc cgccgaagtg gcgctccacc agcggcagcg
      421 tccggttgag gaggttccca aagtcgttcg cgaggtcggc gttgaaccgg tggatgagcg
      481 ccgcgtgcga gtaatccccg tcctgcccaa acgggacctc gcgcagcagc atgtaccgga
      541 aggcgtcggc cgcaacctcc cactcggccc cgctcgcctc cgcgaggcgc gtgatctccc
      601 ggatcggatc gatgaaattc ccccgggtct tgctcatctt ctcgccctcg accgtccaga
      661 acccgtgcgc ccatacctgc ttgggggacg gcagccccac cgcctcgagg aagcacggcc
      721 agatcaccga gtggaagcgg ttgatgtccc ggccgacgag gtgatgcgcc gcgggccaga
      781 accggcggta ccgcgccggg tcgtcgagat acccgatggc ggtcacgtag ttcatcagcg
      841 cctcgatcca gacgtagatg ctctgcgccg gatcgaacgg cacgggaatg ccccacttga
      901 ccgacaggcg gctcacggcc aggtcgcgca atcctccgcg aacgaggctc agcatctcgt
      961 tccgctgggt ctccggctgg ataaacccgg gctcctcctc gatcttgcgg aggatccacg
     1021 gttggtactg gctcaggcgg aagacgtagt tctcctcggc ggtccattcc acggggcgct
     1081 tgtggatcgg gcacagggtc ccctcgagca gttcatcctc cgtgtagaag gcctcgcacg
     1141 acgggcagta ccatccctcg taggtcccct tgtagatcgc gccgcggtcg taggcctgct
     1201 ggaacaggtg ctgggcgagg cggatatggc ggggctccgt cgtcctgatg aagtcgtcgt
     1261 tggtgatccc gagccgcttc cacagcgcct gccactgggg tgcgatgcga tcgcaccaca
     1321 cctgcggggt gacgcccgcc tcgcgggctc tgcgctcgat gttcaccccg tgctcgtcgg
     1381 tgccggtgag gaagaacacc tccgccccgg ccagccgctt ccatcgggcg atcgtatccg
     1441 cggccagcgt ggcgtacgcg tgcccgacgt gcggcacatc gttgtcgtag tagatcgggg
     1501 tcgtgacgta gaacggctcc atcgcgtcgc ccccaaaaac aaacagcctc tcgtcctcgg
     1561 gacgagaggc agccgccttc gtggtaccac ccaggtgcgc ccgcccacgc gggcctcacc
     1621 ggccccggcc tccccatcga gacgccggca gccttagccg gtcacgcagg ccgcgcggcg
     1681 ccgcctaccg tgccggcgcc cgcatgggcc tccggcgatt cagcgtgcag ctccggagcc
     1741 atgttcaccc cgccgccccc cgccggctcg caccaagcac cggctcgctc atccgcgggt
     1801 gacgggctta ctcccgctcc ctcatcgctt tggccccatg ctacggggcg cccgggggaa
     1861 tgtcaagact catcccgacg cgcccgaccc tccaaatgtg agtaaccttg caggccgctg
     1921 gggcgtggcc ggcatctaac cagccgtggc gtcctcctgc caaagcgcga aaacgttgtg
     1981 ttcgggatct gctacgatcg agaaccagcc catcttgggg acaggggatt taggcctcac
     2041 cagcgacccg cccagcttct g
//
LOCUS       DASQGH010000280         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49364, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000280 DASQGH010000000
VERSION     DASQGH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49364"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="VEW91_05485"
     CDS             complement(<1..89)
                     /locus_tag="VEW91_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYM91069.1"
                     /translation="MGKAKFERTKPHVNVGTIGHVDHGKTTLTA"
     gene            complement(145..220)
                     /locus_tag="VEW91_05490"
     tRNA            complement(145..220)
                     /locus_tag="VEW91_05490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Thr,seq:ggt)
     gene            350..1375
                     /locus_tag="VEW91_05495"
     CDS             350..1375
                     /locus_tag="VEW91_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYM91070.1"
                     /translation="MRAELVHAEIDQAQRVAAGPEGAQVYDAYSQAVIGAVERIGPAV
                     VSLGMARPAPEQLKRRGLPELRGAGSGVIITPDGYILTNSHVVRSAHTIEVRLQDGRT
                     FPARLVGDDPYSDLAVIRAPEGGLPLAELGDSSHLRVGQLVVAVGNPLGFQATVTAGV
                     ISALGRTLRAETGRLIENVIQTDAALNPGNSGGPLADSRGTVIGINTAVIAGSQGICF
                     AIPANTAQWVASQLIRDGRVRRAYLGIQAQVIPLDRRVAVAYRVDAATAVRISEVRPD
                     TASAQAGLRVGDLIVRIGDAPVATLDDLMLALGQRAIGESVPVQILRGAERITVEVRP
                     TELPAEA"
     gene            1383..1937
                     /locus_tag="VEW91_05500"
     CDS             1383..1937
                     /locus_tag="VEW91_05500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HYM91071.1"
                     /translation="MAALRVLGIPGSLRRLSYNRGLLLAAQEVAPAGMAIEIADLAAI
                     PLYNADVEAEGDPPPVQAFKAAIAAADALLIATPEYNHLLSGVLKNAIDWASRPPSHT
                     VLRHKPVAVLGASDGAVGTARAQLALRQTLASTESYVLLRPQVLVANARDRFDASGRL
                     TDERTRGSIRALLDALGEWAERFR"
     gene            complement(2261..3133)
                     /locus_tag="VEW91_05505"
                     /pseudo
     CDS             complement(2261..3133)
                     /locus_tag="VEW91_05505"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            complement(3152..>3876)
                     /locus_tag="VEW91_05510"
     CDS             complement(3152..>3876)
                     /locus_tag="VEW91_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91072.1"
                     /translation="ELYITYDDGVRVRCDAGRGHVWVSVLQPRAGNHWLASHLFFTIP
                     FVEILKRRARYSLHAAGLCVDGQALLLPGPSGSGKTTLAVALMQAGFDFLGDDMLFLA
                     ERPEGLSVLAFPDELDIAPETGALLPAVRHRVGSAPAGGTAKHRVPAEDLQASASVWE
                     CRPRVLVFPRVAHSNESTLTRIDAQEALLELASNILLTEPHACQAHLDILGRLVRESV
                     CYRLETGRDFDRLPRLLRRLFV"
BASE COUNT          663 a         1366 c         1310 g          537 t
ORIGIN      
        1 gccgtcagcg tcgtcttccc gtggtctacg tgcccgatcg tccccacatt cacgtgtggc
       61 ttcgtccgct cgaacttcgc cttccccatc cccctgcctc cccgcaaagg tatagatcag
      121 aaacccgccg gcactgcctc gagatggagc ccacgaccgg attcgaaccg gtgaccttgt
      181 ccttaccaag gacacgctct gccgcctgag ctacgtgggc tcgtgcgcag attgattata
      241 acaaccacgg ggggggtgtc aaccgacggg gaatcgagcc gcccgatgcg ttcggaatac
      301 aacccatccg ggtcgcgttg agcggacaga gacctggagg tgagttgacg tgcgcgcgga
      361 gctggtccac gcggagatcg atcaagccca acgggtggcg gcgggcccgg agggcgctca
      421 ggtgtacgat gcctactcgc aggcggtgat cggggctgtg gagcggatcg gcccggccgt
      481 cgtcagcctg gggatggcgc ggcccgcccc cgagcaactg aagcgccgcg ggctgcccga
      541 actccggggg gccgggagcg gcgtgatcat caccccggac gggtacatcc tcaccaacag
      601 ccacgtggtg cgatcggcac acacgatcga ggtccgcctg caggacggcc ggacctttcc
      661 ggcccgcctc gtcggcgacg atccctacag cgatctcgcg gtgatccggg cccccgaggg
      721 gggactgccc ctggccgagc tcggcgattc ctcgcacctg cgggtcgggc agctggtggt
      781 cgccgtgggc aacccgctcg ggtttcaggc gacggtcacc gccggggtca tcagcgccct
      841 cgggcgcacc ctgcgggcgg aaaccggccg gctcatcgag aacgtcattc aaaccgacgc
      901 ggcgctcaac cccggcaatt ccggcggtcc gctcgccgac tcccgaggga cggtcatcgg
      961 gatcaacacc gcggtgatcg ccggctcgca ggggatctgc ttcgcgattc ctgcgaacac
     1021 ggcccagtgg gtcgcgtccc agctcatccg cgacgggcgc gtccggcggg cgtacctcgg
     1081 gatccaggcg caggtgatcc cgctggaccg gcgcgtggcc gtcgcctacc gcgtcgacgc
     1141 ggccaccgcg gtgcggatca gcgaggtgcg ccccgacacc gcatcggccc aggcggggct
     1201 ccgggtgggg gacctgatcg tccggatcgg ggacgcgccg gtggccaccc tggacgacct
     1261 gatgctcgcg ctggggcagc gcgcgatcgg cgaatcggtg ccggtacaga tcctccgggg
     1321 cgcggagcgc atcacggtgg aggttcgccc gaccgagctg ccggcagagg cgtagcacgc
     1381 ccgtggcggc cctgcgggtc ctcgggatcc ccggaagcct gaggcgcctg tcgtacaacc
     1441 gggggttgct gctcgccgcg caggaggttg ccccggccgg gatggccata gagatcgccg
     1501 acctcgcggc gatcccgctg tacaacgcgg atgtcgaggc cgagggagac ccaccgcctg
     1561 tccaagcgtt caaggcggcg atcgccgccg ccgatgcgct gctgatcgcc acccccgagt
     1621 acaaccacct gctttccggc gtgctcaaga acgcgatcga ctgggcctcc cggcccccga
     1681 gccacaccgt gctccggcac aaacccgtcg cggtgctggg agcgtcggac ggcgcggtag
     1741 gcaccgcccg cgcccagctc gccctccgtc agacgctcgc ctcgacggag tcctacgtcc
     1801 tgctccggcc ccaggtgctc gtcgccaacg cgcgcgaccg gttcgacgcc agcggccgcc
     1861 tcaccgacga acggacccgc gggtccatcc gcgcactgct cgacgcgctg ggcgaatggg
     1921 cggagcgctt tcggtagcgt agacgcctca ccagcgggat cagcgcgtat ttggcgagca
     1981 ggcggatgaa tcgcaaggga tagtacgcca cgcgcagcgg cccgggcagg ggcaggagcc
     2041 tcctgtcgtc cgggctcacg gtcaggacac gttgggcaaa gcgcgcacca acccacatcc
     2101 ggtcggaaaa ccgctccacg agctcgaagg ggtggtatgg gcgtgcggcg tcttcctcgc
     2161 tgaaaagctg ggcctgcacc cgagcggcca accgcgaaat gcgcgcatcc tgccggacgg
     2221 agtttaatac ggcgccgggg agaggtgcgc cgaggagatc cgacgcgagt cggaggccca
     2281 gccccaccat gcgcaggcac cccagtcccc gcgcctgggc ctcgacgtcc cgccagttga
     2341 tgtccggatt cgcgccgacc agctccgcga cgtcacaaat ccatttcagc acgccccagt
     2401 agtgcttgtc gccgtgcacg cacagcacca ggagcaggtc ctcggcggtg agggtcggca
     2461 ccgtggcccc atcgagcgtg atcacccggc ggcgccgccc taggcgctcg aaatcaagcg
     2521 ccgcatgcag ataccagggc gtcagccgga aatggatctc cgcgatcgag ccatcaccat
     2581 cgcggcggaa gaaatactcg tacgtccccc gctcggatgg gaacccgtcc cgatcgagcg
     2641 gggtgtgccc gagccggtac cccgcaccgc gcatgacctc ccccgcccgc ggcgcatcgc
     2701 ggcgacggac caagaaatcc agatcgcaga atgtgcgtaa cgacagatcc ccatacgcgg
     2761 caaccgccag ggccggcccc ttgaacggaa gcgcggggat cgagtgcgcg tcaagcaccc
     2821 tgaggagcct gagcaactcc tcggtgaggc ggccggtccg ccggacgtgc tcgtcgaaat
     2881 accgcccgag gtgctcgagg atccccgcgg gcacggcggg gcttccgacg gcccggaggt
     2941 gcacgtagac cagcggcaag agatcgtggc tgagggcgtc ccgcacgaag aagacccagt
     3001 ccaattcccg gtcaagcaac gtcgtgatgc gccgggccgt ctctggaccg atccgggtac
     3061 gcgcgcagag gagcagcagc tccgtttcgg ggcggagcgt gatgggcgcc gatcgttcag
     3121 tgagcacggg cacggcgcgc gcctcgcctc cctacacgaa gagccttcgc aggagccggg
     3181 ggagccgatc gaagtcccga ccggtctcca aacggtagca cacactctcc cgcaccagcc
     3241 gccccaggat atcaagatgg gcctggcagg cgtggggctc cgtgagcagg atgtttgagg
     3301 cgagttcgag gagcgcctcc tgggcgtcga tacgagtgag cgtgctctcg ttcgagtgcg
     3361 cgaccctggg aaacaccagc accctgggtc gacattccca cacggaggcc gaggcctgca
     3421 ggtcctcggc cgggacccga tgcttcgccg ttccacccgc aggagccgac cccacccgat
     3481 ggcgcaccgc cggaagcagc gccccggtct cgggcgcgat gtccagttca tcaggaaagg
     3541 ccagaacgct caggccctcc ggacgttcgg cgagaaagag catatcgtcc ccgagaaagt
     3601 caaaccccgc ctgcatgagg gcgacggcga gggtcgtctt gcccgaccca ctcgggcccg
     3661 gcagcagcaa ggcctgaccg tccacgcaca acccggccgc atgcaggctg tagcgggccc
     3721 gacgcttcag gatctcaacg aatggaatcg tgaagaaaag gtgggaggcc aaccaatggt
     3781 tgccggcccg cggctgcagc accgagaccc acacgtgtcc ccggccggca tcgcaccgca
     3841 ctctcacgcc gtcgtcatag gtgatgtaca gctcat
//
LOCUS       DASQGH010000281         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43676, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000281 DASQGH010000000
VERSION     DASQGH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43676"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VEW91_05515"
     CDS             complement(<1..238)
                     /locus_tag="VEW91_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HYM91073.1"
                     /translation="MAEHPVEPVLPPRTAGMWVQAVRPFSFTASLVPVLLGSAVAATQ
                     GMFHPGLFLLTLLGAVAIQGAANLFSDYFDYRSGY"
     gene            276..1094
                     /locus_tag="VEW91_05520"
     CDS             276..1094
                     /locus_tag="VEW91_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91074.1"
                     /translation="MRRRALLGWAAATAAAAVLGRPRHAAARGRWWSSLVFGVFENHG
                     FDEVAGLPSHRRLAREGTVLARYSAVAHPSGPNYRALISGATWGETETIDTFHPSLAT
                     QAAALHPPIPTYTYHLAGEIAHRHNPFLDLRAPVARVRRGLASLRGDLGTLPAAVVVY
                     VGWGDLDNMHDGTPARADQNLSALLDTLAASSWFATPDREGRYPALFFCYDEDDYTEA
                     NRVFAAWWGRGVRRGFVSQVPHTHYSFCRTVTDNWGLAPLGQAAAAGSISEPWA"
     gene            1113..1886
                     /locus_tag="VEW91_05525"
     CDS             1113..1886
                     /locus_tag="VEW91_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM91075.1"
                     /translation="MGRGFALFAGVPPEVVRASAREAEGLGYSSLWVNHPGSTDGLAA
                     LGLAARETRRIEIGIGVIPLHTRRPDSIVEGVRSNALPLGRLLLGVGSQNPDALKRVR
                     EGIAELRSQLPVRLVVAALGPQMCRVAGEIADAVLFNWLTPEHARRSAEWVRAGAAVV
                     GRQPPKLFAYVRLALGAAARDRLLTEGARYAAIPAYAAHFARMGVKPVETAIAAQSPE
                     ALPPALGKWQGAVDEIVLRAVPGKDTVEEHVALLRAAKP"
     gene            1949..>2267
                     /locus_tag="VEW91_05530"
     CDS             1949..>2267
                     /locus_tag="VEW91_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYM91076.1"
                     /translation="MSEESKAVVRKFYVLLDKGDLGGVSQLLSDNLVWHFAGIPAPFD
                     KQSFTGFLQAFRLAFPDMQHSLDPQIAEGDRVVTSLTFRATHRGELQGIPASGKRVEI
                     RALN"
BASE COUNT          353 a          767 c          796 g          351 t
ORIGIN      
        1 cgtagccgct gcggtagtca aagtagtcgc tgaagaggtt ggccgcgccc tggatcgcca
       61 ccgcgccgag gagcgtgagg aggaacaacc ccggatggaa catcccctgc gtggccgcca
      121 ccgctgatcc gagcaggacc ggcacgagcg acgcggtgaa cgagaacggc cggaccgcct
      181 gcacccacat cccggccgtg cggggcggca gcacaggctc gaccggatgc tcagccatcg
      241 cgactcctcc tggggaatct cgatcccatg atggcatgcg ccggcgggcg ctcctcgggt
      301 gggcggccgc gacggccgcc gccgcggtgc tcggccggcc ccgccacgcc gcggcccggg
      361 gccgatggtg gagcagcctc gtcttcggcg tcttcgagaa tcacgggttc gacgaagtcg
      421 cgggtctccc ttcccaccgt cggctggccc gcgaggggac ggtcctcgcg cgctactctg
      481 cggtcgcgca cccgtccggg ccgaattacc gggccctgat ctccggggcg acgtgggggg
      541 agaccgagac gatcgacacc ttccacccat ccctcgcaac gcaggcggcc gcgctccacc
      601 cgccaatccc aacgtacacg taccacctgg ccggcgagat cgcccaccgg cacaacccgt
      661 tcctcgacct ccgggcgccg gtggctcggg tgcggcgcgg gctcgcctcc ttgagaggcg
      721 accttggaac gctgccggcc gccgtcgtcg tgtacgtcgg gtggggcgat ctcgacaaca
      781 tgcatgacgg cacacccgcg cgcgccgatc agaacctgtc ggcgctgctc gacacgctgg
      841 ccgcatcctc ctggtttgcg accccggatc gcgagggccg gtatcccgcc ctgttcttct
      901 gctacgatga ggacgactac acggaagcga atcgcgtctt cgcggcctgg tggggccgcg
      961 gggtacgacg cgggtttgtg tcccaggtcc cccacacgca ctacagcttt tgccggacgg
     1021 tgaccgacaa ctgggggctc gccccgctgg ggcaggcggc cgccgcgggg tcgatcagcg
     1081 agccctgggc ctgagaacga gaggagacgg tgatgggacg cggctttgca ttgttcgccg
     1141 gtgtgccgcc cgaggtcgtc cgcgcgtcgg cgcgggaggc cgaagggctc gggtacagct
     1201 ccctgtgggt gaatcatccc ggctcgaccg acgggctggc ggcgcttggc ctcgcggcga
     1261 gggagacacg gcgcatcgag atcgggatcg gcgtgattcc tctccacacg cgcagaccag
     1321 acagcatcgt cgagggggtg cgatccaacg ccctgcccct cggtcgtctc cttctgggtg
     1381 tgggaagcca gaacccggat gccctcaaac gtgtgcgcga agggatcgcc gagttgcggt
     1441 cccagcttcc ggtgcgcctc gtcgtggcgg cgctcggtcc ccagatgtgc cgcgtggccg
     1501 gtgagatcgc cgacgcagtg cttttcaact ggctgacccc cgagcatgcg cgtcgctcgg
     1561 ccgagtgggt acgtgccggc gcggccgtgg tgggacgcca gccgccaaag ctcttcgcgt
     1621 atgtccgcct tgccttgggg gccgcggccc gcgacaggct cctgacggag ggggcccgtt
     1681 acgcggcgat cccggcgtac gcggcccact tcgcgcgcat gggggtgaaa ccggtggaga
     1741 cggcgatcgc cgcacagagt ccggaggcgc tcccgccggc gctcgggaaa tggcagggcg
     1801 cggtcgacga gatcgtgctg cgcgcggttc ccggaaagga cacggtcgaa gagcacgtag
     1861 cgcttctccg ggcggcgaag ccgtaggaat gtgggcggaa cggcatctcc agtcgcgccc
     1921 tgtagactga ggagggaggc gaacctgcat gtccgaggag agcaaagccg tggtcaggaa
     1981 gttctacgtg ctcttggata aaggagattt gggtggggtc agccagctgc tctcggataa
     2041 cttggtctgg catttcgccg gtatcccggc gccattcgac aagcaaagtt tcaccgggtt
     2101 cctccaggcg ttccgcctag cgttccccga catgcagcac agcctggatc cccagatcgc
     2161 tgaaggggac cgcgtcgtca cttccctgac attccgcgcc acccacagag gcgaacttca
     2221 gggcatccca gcaagtggaa agcgggtgga gatccgggcc ctaaaca
//
LOCUS       DASQGH010000282         6367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40827, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000282 DASQGH010000000
VERSION     DASQGH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40827"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VEW91_05535"
     CDS             complement(<1..495)
                     /locus_tag="VEW91_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYM91077.1"
                     /translation="MPFGSIAGNAGCQATQESVLATASVLGGDNTPPHPALASLSLTY
                     LTPGNGKAGINMSKMNPVVHFEMPAEDKKRMADFYTKAFGWQTKMLGPEMGEYVLVTT
                     TESEENEGMTGRPKKPGAINGGFYLKTDDPMSQHPSVVIAVDDIKASIKKVEAAGGKI
                     HGEPY"
     gene            complement(519..3719)
                     /gene="carB"
                     /locus_tag="VEW91_05540"
     CDS             complement(519..3719)
                     /gene="carB"
                     /locus_tag="VEW91_05540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYM91078.1"
                     /translation="MPPAEIRKVLVIGSGPIIIGQAAEFDYSGSQACRSLSEEGVEVV
                     LVNSNPATIMTDVETADRVYIEPLTVEFLTRILERERPQGILATLGGQTGLNLAVGLA
                     ESGVLDRLGVRLLGTPLDAIRRAEDRELFREAMGALGEPVPESAIAWTVDEARAQAEA
                     IGYPVVIRPAYTLGGTGGGIARDRQALDQIASRGLGASRIGQVLVERSLAGWKEIEYE
                     VMRDRVDTCITVCNMENLDPMGVHTGDSVVIAPSQTLSDREYQMLRSASLRIIRGLGI
                     EGGCNIQFALDPRSAEYYVIEVNPRVSRSSALASKATGYPIARVAAKIALGRTLEEIP
                     NAVTGKTRASFEPALDYVVIKIPRWPFDKFAGIDRRLGTQMQATGETMAIGRSVEEAL
                     LKALRSLDLATDGLGYPPGRAWSVEEVRQRIAEACDERFFAIAEGLRRGMLPQEIADL
                     SGIDEFFIHKIAQIVAMETSLRGRRDEEILRAAKAMGFADTAIASLWETTAETVRQAR
                     SQAGIRPVYKIVDTCAGEFPAKTPYFYSTYAQEDEGPTGGGARILVLGSGPIRIGQGI
                     EFDYSSVHAVKALRAEGREAILINNNPETVSTDFDVASRLYVEPLTLEDVLHVVEREQ
                     VQGVLVQVGGQTALNLAGPLAQAGVRLMGTAVEDLDTSEDRAKFYTLLHALGIPCPAG
                     GAARSVDEGLAVAARLGYPLLVRPSYVLGGRGMQIVTEEAELAGYLQRALAAHAEHPV
                     LIDTYLNGREVEIDAVSDGDGVFLPGVMEHIERAGVHSGDSLAVFPALHVRADERDQI
                     IAHTVAIARALHVRGFLNIQFVIKDGTVYVLEANLRSSRTVPFVSKAAGTPLVPIAIR
                     VMLGASLADLGYPGVTLLPPPARIAVKAPVFSMEKLSQVDVVLGPEMTSTGEVMGRDV
                     TLPGALYRALVSAGVTIPAHRAILASIADRDKPGAAPLIRRFAELGYAIYATDDTARF
                     LERQGVRAIRVSKNGTDQTALRLIREQAVGVVVNTPTHGKIPGRPGFALRRAAFERRR
                     PCFTSLDTAAAFLDVLTALNQGHIHAPQAATEVSPL"
     gene            complement(3706..4827)
                     /gene="carA"
                     /locus_tag="VEW91_05545"
     CDS             complement(3706..4827)
                     /gene="carA"
                     /locus_tag="VEW91_05545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYM91079.1"
                     /translation="MGARIALEGGLVLVGRSIGAPGWAGGEVVFTTAMTGYQEVLSDP
                     SYRGQLVTMAYPLIGNYGISGEAWESYRPHVAGFIVSEAAGRPHHWRSETGLSAFLVH
                     WNVVGIAGVDTRRLVRHLRTHGLQRGIISTEEVSDADLIARARAFPEIGTLDLVGEVS
                     TPEPRHHPGEGPRVAVLDCGVKWGIVENLHRRGCDTWVLPHRSTAEEILALDPAGLVL
                     SPGPGDPRILSHQVAQVQRLWGRLPIFGICLGHQIIGRAAGAETFKLPYGHRGTNQPV
                     KDLARGRVCVTTQNHGYAVDEASLAHSEVVVTHRNLNDGTVEGLRHRRLPILSVQYHP
                     EGRPGPLDSAYRFEEWLQMIGAPPHPDALPTPASVDAPR"
     gene            complement(5094..5972)
                     /locus_tag="VEW91_05550"
     CDS             complement(5094..5972)
                     /locus_tag="VEW91_05550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HYM91080.1"
                     /translation="MTTSPSAAEAVGAMLWELGVAGVVEDRRSPVAVRLRCYLPPPRV
                     RPALRALRAGIRRLAGYGLDPGRTSVSSRLVAPRRWVRAWRHSARPLRIGRLVVAPTW
                     MRVPARPGRVVIRIDPGMAFGSGAHPSTRLCLRALLRYMRTRAPGPPALRPTVVDVGA
                     GSGILAIVAARLGARRVWARDIDPVAVAVARENIRANGVARVVRVARGSGLGPSPGRC
                     DLIVANITADAIITLLPSAAARLAPGGMFVGSGVVEDRLGDVLKAGAASDLAPLAVLA
                     SGEWRAVILAERSASP"
     gene            complement(6047..>6367)
                     /locus_tag="VEW91_05555"
     CDS             complement(6047..>6367)
                     /locus_tag="VEW91_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYM91081.1"
                     /translation="DLHCEVPVSMAQAALGDQIEIPTLDGPISFTIQPGLQPGSTATL
                     RGKGMPGLRNGPGDLIVHFPVLIPKDLTKEQEQLLLQFAKLRGQEVAPPKKTLLGKFR
                     PRVS"
BASE COUNT          979 a         2249 c         2163 g          976 t
ORIGIN      
        1 atacggctcg ccatgtattt tgccgccggc cgcttccacc ttttttatgc tcgctttgat
       61 atcgtcaacc gcgataacaa cggagggatg ctggctcatg ggatcgtctg ttttcaggta
      121 aaaaccgcca ttgatcgcac caggtttttt ggggcgaccc gtcattccct cgttctcctc
      181 ggattcggtt gttgtaacga gcacatactc tcccatttcc ggtccgagca ttttcgtctg
      241 ccagccaaac gctttcgtat aaaaatcggc catgcgcttt ttgtcttccg ccggcatttc
      301 aaaatggact acaggattca ttttgctcat atttatgcct gcctttccat ttcctggtgt
      361 taagtatgtt agagaaagag aagctagagc cggatgcgga ggtgtattat ctccgccgag
      421 gacagatgca gtcgcgagga cgctttcctg ggtcgcctgg caaccagcat tgcctgcgat
      481 gctaccaaat ggcacgcgcc gagacaagcg gcggagggtt acagggggga gacctcggtg
      541 gccgcctggg gggcgtggat gtgcccctgg ttgagggccg tcagcacatc caggaacgcc
      601 gcggcggtat cgagcgaggt gaagcacggg cggcgacgct cgaacgcggc ccggcgcagc
      661 gcaaaccccg gccggccggg gatcttcccg tgggtcgggg tgttgaccac cacgcccacc
      721 gcctgctcgc ggatcaggcg cagcgcggtc tggtcggttc cgttcttgct cacccggatc
      781 gcgcgcacgc cctggcgctc gagaaaccgc gccgtgtcgt ccgtggcgta gatcgcgtag
      841 cccagttcgg caaagcgcct gatcagcggg gcggccccgg gcttgtcgcg gtcggcgatg
      901 ctggcgagaa tcgcccggtg cgccgggatc gtcaccccgg cggagacgag cgcgcggtac
      961 aacgcgccgg gcagggtcac gtcccggccc atcacctcgc cggtcgaggt catctccggg
     1021 ccgaggacca cgtccacctg gctcagcttc tccatgctga acaccggcgc cttgacggcg
     1081 atgcgggccg ggggcgggag cagcgtgacc cccgggtacc cgaggtcggc gagcgacgcc
     1141 cccagcatca cccggatcgc gatcggcacg agcggggtgc cggcggcctt gctcacgaac
     1201 ggcaccgtcc ggctgctccg gaggttggcc tccaggacat agaccgtccc gtccttgatc
     1261 acaaactgga tgttgaggaa gcctcggacg tgaagcgcgc gggcgatcgc caccgtgtgg
     1321 gcgatgatct ggtcgcgctc atccgccctc acgtgcagcg caggaaacac ggccaggctg
     1381 tcgcccgagt gcaccccggc ccgctcgata tgctccatca cccccggcag aaacacgccg
     1441 tcgccgtcgc tcaccgcgtc gatctcgacc tcccggccgt tgaggtaggt gtcgatcaac
     1501 acgggatgct ccgcgtgcgc cgcgagcgcg cgctggaggt agccggcgag ttccgcctcc
     1561 tcggtaacga tctgcatgcc ccgcccgccg aggacgtacg agggacggac gaggagcggg
     1621 taccccaggc gggccgcgac cgcgagtccc tcgtccaccg agcgggccgc cccgccggcg
     1681 gggcacggga tcccgagggc atggagcagc gtgtagaact tcgcgcggtc ctcggaggtg
     1741 tcgagatcct ccacggccgt ccccatgagc cgcaccccgg cctgggcgag cggcccggcg
     1801 aggttgaggg cggtctggcc cccgacctgc acgagcaccc cctgcacctg ctcgcgctcg
     1861 acgacgtgga ggacgtcctc cagggtcagg ggctcgacat acagccggct ggcgacgtcg
     1921 aagtcggtgc tcaccgtctc cgggttgttg ttgatcagga tcgcttcccg gccttccgcc
     1981 cgaagcgctt tgaccgcgtg gacgctggaa tagtcgaact cgatcccctg cccgatccgg
     2041 atcgggccgg agccgagcac gaggatccgc gcccctcccc ccgtggggcc ctcgtcctcc
     2101 tgcgcgtacg tcgagtagaa atacggggtc ttcgcgggaa actcgccggc gcaggtatcc
     2161 acgatcttgt agaccggacg gatgccggcc tgggatcggg cctgccgcac ggtctccgcg
     2221 gtcgtctccc agagcgatgc gatcgccgtg tcggcaaacc ccatcgcctt ggccgcccgc
     2281 aggatctcct cgtcgcggcg accgcgcagg ctcgtttcca tcgccacgat ctgcgcaatc
     2341 ttatggatga aaaactcgtc gatgccggag aggtcggcga tctcctgggg gagcatcccg
     2401 cgccggagcc cttccgcgat cgcgaaaaag cgttcatcgc acgcctccgc gatccgctgc
     2461 cggacctcct cgaccgacca ggcgcggccg ggcggatagc cgagcccgtc ggtcgcgagg
     2521 tcgagcgacc gcagggcctt gagcagcgcc tcttcgaccg accgcccgat cgccatggtc
     2581 tcgccggtcg cctgcatctg ggtccccagc cggcggtcga tgcccgcgaa cttgtcgaag
     2641 ggccaccggg ggatcttgat caccacgtag tccaatgccg gctcgaagct ggcccgcgtc
     2701 tttccggtca ccgcgttggg aatctcttcc agcgtccgcc ccagggcgat cttcgcggcc
     2761 acgcgggcga tcggatagcc ggtggccttg ctcgccaggg cgctcgaccg gctcacccgc
     2821 gggttgacct cgatcacgta gtactcggcg ctccggggat cgagggcgaa ctggatgttg
     2881 caccccccct cgatcccgag cccccgaatg atccgcaggc tggcgctgcg gagcatctga
     2941 tactctcgat cgctcagcgt ctggctgggc gcgatgacga cgctgtcgcc cgtgtggacg
     3001 cccatcggat ccaggttctc catgttgcag acggtgatgc aggtatccac gcggtcgcgc
     3061 atcacctcgt actcgatctc cttccacccg gcgagcgacc gctcgaccag gacctgcccg
     3121 atccggctcg ccccgagccc ccgcgacgcg atctggtcga gggcctgccg gtctcgggcg
     3181 atccccccgc cggtcccgcc caacgtatag gccggccgga tcaccaccgg gtagccgatg
     3241 gcttcggcct gggcgcgcgc ctcatccacc gtccaggcga tcgcgctctc cgggaccggc
     3301 tcccccagcg cccccatcgc ctccctgaac aactcgcggt cctccgcccg gcggatcgcg
     3361 tcgaggggcg tgcccaagag ccgcaccccg agccgatcga ggacgccgga ctccgcgagc
     3421 ccgaccgcga ggttgaggcc cgtctgcccg cccagggtgg cgaggatccc ctgggggcgc
     3481 tcccgctcga gaatgcgcgt gaggaactcc accgtcagcg gctcgatgta gactcggtcg
     3541 gcggtttcga cgtcggtcat gatcgtggcg gggttgctgt tgaccagcac cacctccacg
     3601 ccttcctcgg agagcgaccg gcacgcctgg ctgccggagt agtcgaactc ggcggcctgg
     3661 ccgatgatga tcggccccga gccgatcacg aggaccttgc ggatctcagc ggggggcatc
     3721 gacgctcgcc ggcgtgggaa gggcgtcggg gtgcggcggg gcaccgatca tctgcagcca
     3781 ctcctcgaac cggtaggcgg aatcgagcgg cccgggccgc ccttcggggt ggtactggac
     3841 cgataagatc ggcagacgcc ggtgccgcaa tccctccacg gtgccgtcgt tgaggttgcg
     3901 atgggtgacg acgacctccg aatgcgccag cgacgcttca tccacggcgt acccgtggtt
     3961 ttgggtggtc acgcacacgc ggccccgcgc cagatccttc accggctgat tggtcccgcg
     4021 gtgcccgtac gggagcttga acgtctcggc ccccgcggcc cggccgatga tctggtgccc
     4081 gagacagatg ccgaagatcg ggaggcgccc ccacaggcgc tggacttggg ccacctggtg
     4141 cgagagaatc cggggatctc ccggtccggg tgaaagcacc aggccagccg ggtcgagagc
     4201 caggatctct tccgcggtgc tgcggtgggg caacacccag gtgtcgcacc ccctgcggtg
     4261 caggttctcg acgatccccc acttcacccc gcagtcgagc accgccaccc ggggcccctc
     4321 ccccgggtgg tgccggggtt ccggggtgga gacctcgccc acgaggtcga gcgtcccgat
     4381 ctccgggaac gcgcgggcgc gggcgatgag gtccgcgtcg gagacctcct cggtggagat
     4441 gattccccgc tggaggccgt gggtgcggag gtgccgcacc aggcgccgcg tgtccacccc
     4501 tgcgatcccg accacgttcc agtggacgag gaacgcgctc aggccggtct cggaccgcca
     4561 gtggtgcggc cggcccgccg cctcgctgac gataaacccc gccacgtgcg gccgatacga
     4621 ctcccatgcc tcgccgctga tgccgtagtt gccgatcagc ggataggcca tcgtgacgag
     4681 ctgcccccgg taggacggat ccgagaggac ctcctggtag cccgtcatcg ccgtcgtgaa
     4741 cacgacctcc cccccggccc atcccggcgc gccgatggac cggccgacga gcaccagacc
     4801 gccttccagg gctatccgcg cccccacgcg accctccccc cgacacacct atgcataatt
     4861 atactcagtc cattcataaa aatgcaatgg gcgggttccc cgcggggtgg gcccccggag
     4921 ggcaggcggc cgatcgggcc acaaaaaaag aggagccccc ggctccccga cgctcaccca
     4981 caacgtatgc acttgggccg acccgcgcgc accatcgatc cctcccggag atcattaatg
     5041 gttcgcctac cccattgtag cggggcggag gggaattgca aggggtcgga gggctaggga
     5101 gacgcgctcc gctcggcaag gatcaccgcc cgccattcgc ccgacgcgag gactgcgagg
     5161 ggcgccaggt cggaggcggc gccggccttg agcacatcgc ccagacggtc ctcgacgacc
     5221 ccggacccga caaacattcc cccgggggcg agccgtgccg ccgcggaggg gagcagggtg
     5281 atgatcgcgt cggcggtgat gttggccacg atcaggtcgc accttccggg ggaaggcccg
     5341 agcccggatc cccgggcgac gcgcacgacg cgggcgacgc cgtttgcgcg gatattttcg
     5401 cgggccaccg cgacggccac cgggtcgatg tcgcgggccc acacccggcg cgcgcccaga
     5461 cgcgcagcga cgatggccag gatgccggat cccgcgccga cgtcgacgac ggtcggccgc
     5521 agggcgggcg ggccgggcgc tcgggtgcgc atgtaccgca agagggcgcg gaggcacagg
     5581 cgcgtgctcg ggtgcgcgcc gctcccgaag gccatcccgg gatcgatgcg gatgacgacc
     5641 cgcccggggc gcgccgggac gcgcatccag gtcggtgcga cgaccagccg accaatgcgc
     5701 aggggtcggg ccgagtgtcg ccacgcccgg acccaccgcc gcggggccac gagccgggag
     5761 gacacagacg tccggcctgg atcgagcccg tagcccgcca ggcgccggat gcccgcccgg
     5821 agcgcccgca gcgccgggcg cacgcgcggg ggtggcaggt agcaccgcaa gcggaccgcg
     5881 accggggaac gccggtcctc gaccactccc gcgacgccga gttcccacag catcgcgccc
     5941 accgcctcgg cggcggacgg agacgtggtc acgccgacct cggccacgga ccggccgacg
     6001 ggggggcgga cgggccggcg gggggctccc gcacccccac ggggggtcaa gaaacgcggg
     6061 ggcgaaactt cccgagcagc gtcttctttg gcggggcgac ctcctgcccg cgcagcttgg
     6121 cgaactgcag cagcagttgc tcttgctctt tcgtcaagtc cttggggatg aggacgggga
     6181 agtggacgat gaggtcgccg gggccgttgc gcaggcccgg catgcccttt ccccgtagcg
     6241 ttgccgtgct cccgggctgg agcccgggct ggatcgtgaa cgagatcggc ccgtccaggg
     6301 tggggatctc gatctgatct cccaaggcgg cctgggccat cgagacgggg acctcgcagt
     6361 gcagatc
//
LOCUS       DASQGH010000283         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_54994, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000283 DASQGH010000000
VERSION     DASQGH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54994"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VEW91_05560"
     CDS             complement(<1..1073)
                     /locus_tag="VEW91_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HYM91082.1"
                     /translation="MIWNAPAETMPRAELAALQLSRLQALAQRVYERVPFYRQAFDRA
                     KVHPEQIRTLDDLRRLPWTRKADLRDHYPFGLFAVPRDQVVRVHASSGTTGKPTVVGY
                     TRGDLGVWAEVCARCLAASGGRPGDVFHNAYGYGLFTGGLGMHYGGELLGMTVVPVSG
                     GNTERQLLLIQDFQPRVIACTPSYMLTLAEAARARGLDPRRFSLRYAVLGAEPWTEAM
                     RQQIQRLLPVRAVNIYGLSEVIGPGVSNECVEAQRGSHVFEDHFIAEVLDPASGDPVP
                     PGEVGELTFTTLTKEALPVIRYRSGDLASLDSSPCACGRTHARMSLVVGRTDDMLIIR
                     GVNVFPSQVESVVVDFAELSPHY"
     gene            complement(1084..1602)
                     /locus_tag="VEW91_05565"
     CDS             complement(1084..1602)
                     /locus_tag="VEW91_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HYM91083.1"
                     /translation="MLHYGGIPRIDLATWEFFVAGEVEAPRRWTGEEFTRLPRTTVRC
                     DIHCVTAWSRFNNTFEGVSAAEVLRQGRVRPEATFVMVESFGGYTTNLPVAALLDDDG
                     VFAFTHDGQPLTPEHGGPCRLVVPKLYFWKSAKWVRGLTLLREDRPGFWEQNGYHMRG
                     DPWKEERYSDPW"
     gene            complement(1679..>2311)
                     /locus_tag="VEW91_05570"
     CDS             complement(1679..>2311)
                     /locus_tag="VEW91_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM91084.1"
                     /translation="SAWPGGRGELPAIIGFLRAHAPGFENAELLRTADYLYIRETRHI
                     RGLYTLTVHDIVDSRVFWDAIGVASYPIDLHPYHPGEFNPYAAYRYVYTIPLRALVPH
                     GVDNLLVASRSISATYEAAGSARVAPTTMEEGQAAGVAAVLSMRARIAVPRFTEQPPL
                     VHELQETLYAQGAYLLPETIAAAGGSSPGRSAGPTQVLPRPQAPDPLAPQ"
BASE COUNT          365 a          798 c          811 g          337 t
ORIGIN      
        1 tggtagtggg gcgaaagttc ggcgaagtcg acgaccaccg actcgacctg ggagggaaag
       61 acgttgaccc cgcggatgat cagcatgtcg tccgtccggc cgaccaccag cgacatccgc
      121 gcatgggtcc gcccgcaggc gcacggcgac gaatccagcg aggcgaggtc ccccgaccgg
      181 taccggatca cgggcagcgc ttccttggtg agcgtcgtga atgtgagctc gccgacctcg
      241 cccgggggca ccgggtctcc ggaggcgggg tcgagcacct cggcgatgaa gtggtcctcg
      301 aagacatgcg agccgcgctg cgcttcgacg cactcgttgc tgacccccgg tccgatcacc
      361 tcgctcagcc catagatgtt gaccgcgcgg accgggagca gccgctggat ctgctggcgc
      421 atcgcttcgg tccacggctc cgcccccagc acggcgtacc ggagggaaaa ccgccggggg
      481 tcgaggccgc gcgcgcgcgc cgcctcggcc agcgtcagca tgtacgaggg cgtgcaggcg
      541 atgacgcggg gctgaaaatc ttggatcagc agcagctgcc gctcggtgtt gccgccggac
      601 accgggacga cggtcatgcc gagtagctcg ccaccgtagt gcatcccgag gccgccggtg
      661 aagagcccgt atccgtaggc gttgtggaat acgtcgccgg gccggccacc gctggcggca
      721 aggcaccgcg cgcacacctc cgcccagaca ccgagatccc cgcgggtata cccgaccacc
      781 gtgggctttc cggttgtccc cgaggacgcg tggacgcgga ccacctggtc ccgcggcacc
      841 gcgaacaggc cgaacggata gtgatcccgc aggtccgcct tgcgtgtcca ggggaggcgc
      901 cggaggtcgt cgagcgtgcg gatctgctcg gggtgcacct tcgcccggtc gaacgcctgg
      961 cggtagaacg gcacccgctc gtacacccgc tgggcgaggg cctgaaggcg gctcagctgc
     1021 agcgcggcga gttcggccct cggcatcgtc tcggcgggag cgttccagat catcggagga
     1081 tgtctaccag gggtcggagt accgttcctc cttccacgga tcgccccgca tgtggtaccc
     1141 gttttgctcc cagaaccccg ggcggtcctc ccgcaggagg gtgagccccc ggacccactt
     1201 cgcgctcttc cagaagtaga gcttcggcac cacgaggcgg cagggtccgc cgtgctcggg
     1261 ggtgagcggc tggccgtcat gcgtgaacgc aaacacgccg tcatcgtcca gcagggccgc
     1321 aaccggcagg ttggtcgtgt atccaccgaa gctctcgacc atcacgaacg tggcctccgg
     1381 ccggacccgg ccctggcgca ggacctcggc ggccgacacg ccctcaaagg tgttgttgaa
     1441 gcgggaccag gcggtcacgc agtggatgtc gcaccgcacg gtggtccggg gcaggcgggt
     1501 gaactcctcc cccgtccagc gccgcggcgc ctccacctct ccggccacga aaaactccca
     1561 cgtggccaga tcgatccggg ggatcccccc gtaatgcaac accggccact tctgggtctc
     1621 gtactgcccg gggggtaacc gcgaccctgc tgcgtgcagc ggcgtcacac cgaaggggtt
     1681 attggggagc gagggggtct ggcgcctggg gccgcggaag cacctgcgtg ggaccggcgc
     1741 tgcgcccggg cgaactgccg cccgccgccg cgatggtttc gggcaggaga taggcgcctt
     1801 gcgcgtacag cgtctcctgc agctcgtgga ccagcggcgg ctgctccgtg aatcggggca
     1861 ccgcgatccg ggcccgcatc gagagcaccg ccgccacacc ggcggcctgc ccctcctcca
     1921 tcgtcgtggg cgctacgcgg gccgatcccg cggcctcgta cgtggccgag atcgaccggc
     1981 tcgcaaccaa gaggttgtcc accccgtgcg ggaccagggc ccggagggga attgtgtaga
     2041 cataccggta ggccgcgtac gggttgaatt ccccggggtg ataggggtgc aggtcgatcg
     2101 gatagctcgc cacgccgatc gcatcccaga acacgcggga gtccacgatg tcgtgcacgg
     2161 tcagggtgta caggccgcgg atgtggcggg tctcccggat gtacaggtaa tcggcggtcc
     2221 gaagcagttc ggcgttctca aacccggggg cgtgggctcg gaggaacccg atgatcgcgg
     2281 gcagctcccc ccgccccccc ggccaggccg a
//
LOCUS       DASQGH010000284         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38159, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000284 DASQGH010000000
VERSION     DASQGH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38159"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VEW91_05575"
     CDS             complement(<1..333)
                     /locus_tag="VEW91_05575"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HYM91085.1"
                     /translation="MDKEAIQKAVLTILRAIGESPEREGLADTPRRIAEMYEELFSGI
                     HQDPAALLATGFEEERHKEMVVVKDIPFTSMCEHHLMPIRGRAHVGYIPQGRIVGVSK
                     IARVVEVLA"
     gene            complement(380..1543)
                     /gene="folP"
                     /locus_tag="VEW91_05580"
     CDS             complement(380..1543)
                     /gene="folP"
                     /locus_tag="VEW91_05580"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYM91086.1"
                     /translation="MPPQEGRLWARVVRWPAPPGNGARGAYAVKLHGLTPAALAAVAG
                     AAASLGVRTSSGPGELVVEGPVEGFGRLAPQAAGREAVEAARRAVERFRAQPVLRACG
                     RTFDLARPLVVGILNATPDSFYDQGRYFDRAAAIARAYEMVAEGADLIEVGGETARPG
                     PAVDPEEETRRVVPLIEALARHLPVPVSVDTYKPEVARRAVGAGAVLINDISGLADMR
                     MAGIAAASGAALVVMHIQGRPKVRQVSPRYRAVTDDVYAFLEARTQAAEAAGVPRARL
                     VVDPGFSFGKAPDHDLEILRRLGEFRSLGYPIYLATSRKNYIRDLLGLPVEELLEGTA
                     AAVAYGLAQGAHLVRTHDVRFTARLVRMMHAILHPGGEERRESGEEVEARGKA"
     gene            complement(1543..3456)
                     /gene="uvrC"
                     /locus_tag="VEW91_05585"
     CDS             complement(1543..3456)
                     /gene="uvrC"
                     /locus_tag="VEW91_05585"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HYM91087.1"
                     /translation="MIPHAAQDSEFPPAAAPEAAVTSALAEKLGTLPDQPGVYQMKDG
                     AGRVLYVGKAASLRARVRQYFQAGHTDSPRILHLISKIRDVETVVCANEVEALILEAT
                     LIKRHHPWYNVRLADDKAYPYLKLTRDPFPKIVMTRKVLRDGGKYFGPYPYHEPKLVG
                     RTIRLIRRLFKLRTCNLEITGDLPRPCLDYYIGQCSAPCVAWGASKDAYAEQVRNAAA
                     FLEGKQDDLLGDLHRAMESAAAAMNFERAAQLRDQIRGLEALGEKQRIVSSGGEDRDI
                     LALAQDGDLGCAQLFFVRGGRLIGQEHFLLRGTRGVGGAESLGAFLPQFYETAASIPP
                     QILLPEPVPDRAVIERWLTERRGGRVELVTPQRGERVRLVAMARENAALHLAEERARI
                     GDAAGPGVRDLQDLLRLEAPPVRIECYDISNFQGGESVAALVVAEGGRPKKRDYRRFR
                     MKSTEGPDDTAMMQEVLRRRFAQARKEQERLDRDEPIPVKWAALPDLIVVDGGRGQLN
                     AALEVLFEYNHAIPVIALAKQQELIYVKDLTEPVGLPRESPALQLLQRLRDEAHRFAN
                     AYHQKLRERRVVFSALDEIAGVGERRKRALIRHFGSVRTIRAASVEEIAAVDGIGPKV
                     ADRIHRYLQEHPA"
     gene            complement(3488..4261)
                     /locus_tag="VEW91_05590"
     CDS             complement(3488..4261)
                     /locus_tag="VEW91_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYM91088.1"
                     /translation="MTKNTAKARLREGGTVVGTMVSEMWTEEVAHVLAAAGFDFLVLD
                     TEHGSANPESLQRISRAARSAGIPPLVRVPDIGYSFIARTLDTGALGVMVPHVETVAD
                     AREIVRCVKYPPVGERGFGLRAAVTDYRGPSVAEAIAWANAETMIMAQVESRRCLDHL
                     DEIAAVPEIDVLLIGPTDLSISLGVPGQMLHPTMLEAYRHVVAVANRHGRAAGIHPSD
                     LKVVQQGRELGMRLLMYASDIRLLLGAARQAVEALRPPQ"
     gene            complement(4258..>4592)
                     /locus_tag="VEW91_05595"
     CDS             complement(4258..>4592)
                     /locus_tag="VEW91_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM91089.1"
                     /translation="TSRGKVVDEAALIAALRSGRLGGAGLDVLWTEPPAKDHPLFQLD
                     QVVLTPHYASSTVEAMDDLAHKINRQIVQYLRGEWPTYLANPAVRRQAGCRLPAAHST
                     REIQGGGR"
BASE COUNT          622 a         1690 c         1602 g          678 t
ORIGIN      
        1 cgcgagcacc tcgacgaccc tcgcgatctt gctgaccccg acgatgcgcc cctgggggat
       61 gtagccgacg tgggcccgcc cgcggatggg catgagatgg tgctcacaca tcgatgtgaa
      121 ggggatatcc ttgaccacca ccatctcctt gtgccgctcc tcttcgaacc cggtggcgag
      181 gagcgcggcg gggtcctggt ggatcccgga gaagagctct tcgtacatct cggcgatccg
      241 ccggggggtg tcggcgagcc cctcccgctc cggcgactcg ccgatggcgc ggaggatcgt
      301 cagcaccgcc ttctggatgg cctctttgtc cacgcccgcc ccgacgccca ggccgcgccc
      361 ggctccgtcg ggatgcgtgc tacgccttgc cacgagcctc cacctcctcc ccgctctccc
      421 gacgctcttc cccgccggga tgcaggatcg cgtgcatcat gcggaccagc cgcgcggtga
      481 accggacatc gtgggtccgg accaggtgcg cgccctgcgc cagaccgtag gcgaccgcgg
      541 cggccgtgcc ctcgagcagc tcttccacgg gcagcccgag caggtcgcga atatagtttt
      601 tccgcgaggt cgcaagatag atcggatagc cgaggctgcg aaactccccc agccggcgga
      661 ggatctccag atcgtggtcg ggcgccttcc caaagctgaa cccggggtcg acgaccagcc
      721 gtgcgcgggg cacgcccgcc gcctccgccg cctgggtgcg ggcttcgaga aacgcgtaca
      781 cgtcgtctgt gaccgcgcga tagcgcgggc tgacctggcg gacctttggg cggccctgaa
      841 tgtgcatgac cacgagcgcg gccccggacg ccgccgcgat cccggccatc cgcatgtcgg
      901 cgagcccgct gatgtcgttg atcaggacgg cccccgcccc caccgcccgc cgggccacct
      961 ccggcttgta cgtatccacg ctgaccggca ccggcaggtg cctggccagc gcctcgatca
     1021 gcggtacgac ccggcgggtt tcctcctccg ggtccaccgc gggcccgggc cgggccgtct
     1081 ccccgccgac ctcgatcagg tcggcgcctt ccgcgaccat ctcataggcg cgggcgatcg
     1141 cggcggcccg atcaaagtac cggccctggt cgtagaacga atcgggggtc gcgttcagga
     1201 tcccgacgac gagggggcgc gccaggtcaa aggtccggcc gcacgcgcgg agcacgggct
     1261 gcgcccgaaa ccgctccacc gcccggcggg cggcctccac ggcctcgcgg ccggcggcct
     1321 ggggggcgag ccgcccaaac ccctcgaccg ggccttcgac gacgagttcg cccgggccgc
     1381 tcgacgtccg cacgccgagg gaggcggccg cgccggccac cgccgccagc gcggccgggg
     1441 tgaggccgtg gagcttgacc gcgtacgcgc cccgggcgcc attcccgggc ggggcgggcc
     1501 agcgcaccac ccgggcccag aggcggccct cctgcggggg cattaggccg gatgctcctg
     1561 gaggtatcgg tggatccggt cggcgacctt cgggccgatc ccgtccaccg cggcgatctc
     1621 ctcgacgctc gcggcccgaa tggtccggac cgatccgaag tggcggatca gggcccgctt
     1681 cctccgctcc cccacgcctg cgatctcgtc gagcgccgag aagacgaccc gccgctcccg
     1741 gagcttctga tgataggcgt tggcaaaccg gtgggcttcg tcgcggagcc gctgcagcag
     1801 ctgcagcgcc ggcgactcgc ggggcagccc caccggctcg gtgagatcct tgacgtagat
     1861 gagctcctgc tgcttggcca gggcgatcac cgggatcgcg tggttgtact cgaacagcac
     1921 ctcgagcgcg gcgttgagct gtccccgccc cccgtcgacc acgatcagat cgggcagcgc
     1981 cgcccatttc accgggatcg gctcgtcgcg gtccaaccgc tcctgttcct tgcgggcctg
     2041 ggcgaaccgc cggcggagga cctcctgcat catcgcggtg tcgtccggcc cttccgtgga
     2101 cttcatgcgg aaccggcggt agtcgcgctt cttgggccgg cccccttcgg ccacgacgag
     2161 ggcggccacg gactccccgc cctggaagtt gctgatgtcg tagcactcga tccgcaccgg
     2221 gggcgcctcg aggcgcagca ggtcctgcag gtcccggacc cccggcccgg cggcatcgcc
     2281 gatgcgggcc cgctcctcgg ccaggtgcag cgcggcgttc tcgcgggcca tcgcgaccag
     2341 ccggacccgc tccccgcgtt ggggcgtgac cagctccacc cgcccgcccc gccgctccgt
     2401 cagccaccgc tcgatcaccg cgcggtccgg gaccggctcg ggcaggagga tctgcggggg
     2461 gatcgaggcc gcggtctcgt agaactgcgg caggaaggcg ccgagcgact cggccccccc
     2521 gaccccgcgg gtgccgcgca gcaggaagtg ctcctgcccg atcaggcgac cgccccgcac
     2581 gaagaagagc tgcgcgcagc ccagatcgcc gtcctgggcg agcgcgagga tatcccgatc
     2641 ctcgccgccc gaggacacga tgcgctgctt ctccccgagg gcttccagcc cccggatctg
     2701 gtcgcggagc tgcgccgcgc gctcgaagtt catcgccgcg gcggcggact ccatcgcgcg
     2761 gtggaggtcg cccaggagat cgtcctgctt gccctccagg aaggccgccg cgttccggac
     2821 ctgctccgcg tacgcgtctt tgctcgcgcc ccaggcgacg cacggggcac tgcactggcc
     2881 gatgtagtag tccaggcacg gcctcggcag atccccggtg atctcgagat tgcaggtgcg
     2941 gagcttgaac agccgccgga tcagccggat cgtccgtccg acgagcttcg gctcgtgata
     3001 cgggtacggg ccgaagtact tgcccccgtc gcggaggacc ttgcgggtca tgacgatctt
     3061 cgggaaggga tcgcgggtga gcttcaggta cgggtaggcc ttgtcgtcgg ccagacggac
     3121 gttgtaccag gggtggtggc gcttgatgag ggtggcttcg aggatcagcg cctccacctc
     3181 gttggcgcac accacggtct cgacatcccg gatcttcgag atcaggtgga ggatccgggg
     3241 gctgtcggtg tgccccgcct ggaagtactg ccggacgcgg gcgcgcaggg aggcggcctt
     3301 gccgacatag agcacccggc cggccccgtc cttcatctgg tagacgccgg gctggtccgg
     3361 gagcgtgccg agcttctccg cgagggcgga cgtcaccgcc gcctccggcg cggcggccgg
     3421 cgggaactcg ctgtcctgcg ctgcgtgcgg gatcatctct cctccattat agcggaggag
     3481 accgcggcta ctggggcggg cggagcgcct ccaccgcctg gcgggccgcc ccgagcagca
     3541 gccggatgtc cgaggcgtac atcaggagcc gcatccccag ctcccgcccc tgctggacga
     3601 ccttaaggtc cgacgggtgg atcccggcgg cgcggccgtg gcggttggcc acggcaacca
     3661 catgccggta ggcctccagc atcgtggggt gcagcatctg gccgggcacg cccagcgaga
     3721 tgctcaggtc cgtggggccg atcaggagga cgtcgatctc cgggacggcg gcgatctcgt
     3781 ccaggtggtc gaggcagcgg cggctctcca cctgcgccat gatcatcgtc tccgcgttgg
     3841 cccacgcgat cgcctcggcc accgaggggc cccggtagtc ggtgaccgcg gcccgcagcc
     3901 caaacccgcg ctcgcccact ggcgggtact tgacgcagcg cacgatctcc ctggcatcgg
     3961 ccaccgtctc gacgtgcggc accatgacgc cgagggcccc ggtgtcgagc gtgcgcgcga
     4021 tgaacgagta cccgatatcg gggacccgca cgagcggggg gatgcccgcg ctccgcgccg
     4081 cgcggctgat gcgctggagg ctctccggat tcgccgagcc gtgctcggtg tcgagcacga
     4141 ggaagtcgaa gccggccgcc gcgaggacgt gggcgacctc ctcggtccac atctccgaca
     4201 ccatggtgcc gacgacggtg ccgccctcgc gaagccgcgc cttggcggtg ttcttggtca
     4261 tcgtcctccc ccctgtatct cccgggtcga gtgggcggcg gggagccggc acccggcctg
     4321 ccgacgcacc gccggattgg ccagatacgt cggccactcg cccctgaggt actgcacgat
     4381 ctgccggttg atcttgtggg ccaggtcgtc catcgcttcg acggtggagg aggcatagtg
     4441 cggggtcagc accacctggt ccagctggaa cagcgggtgg tccttggccg gcggttcggt
     4501 ccacagcacg tccagccccg ccccccccag ccggcccgac cgcagggcgg cgatgagggc
     4561 ggcctcgtcg acgaccttgc cccgcgacgt gt
//
LOCUS       DASQGH010000285         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46608, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000285 DASQGH010000000
VERSION     DASQGH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46608"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VEW91_05600"
     CDS             <1..480
                     /locus_tag="VEW91_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91090.1"
                     /translation="ARSPMPTARNHMGDGGSVNGKIYVIGGRLSGAFIIAMPGNTDLV
                     QEYDPAADAWATKTPMPTARSGLNSATLNGIIYVAGGEVRTYQYLAAFRAFEAYDPAS
                     NTWWQLPPMPSPRHECAMAALGNRIHIVGGAVQSAIVPPVQGVSFGTPAHDAFEIAL"
     gene            525..1790
                     /gene="cofG"
                     /locus_tag="VEW91_05605"
     CDS             525..1790
                     /gene="cofG"
                     /locus_tag="VEW91_05605"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HYM91091.1"
                     /translation="MTTVSPQRLSAFLDPGRIARTSAVARVLDRTLDGVPLTRDDAVL
                     LITCPEEDVPAVCAAARVLRARGKGRVVSFSPKVFIPLTRLCRDACGYCTFRTDPRSD
                     PRLYMTPEDVLAVARAGERLGCTEALFTLGERPEQRFPEARRWLAARGHRSTLSYLRE
                     MADRVLRETALLPHLNPGTMSHAEMAALRDVSASMGLMLETVSPRLCSPGGPHEHAPS
                     KHPRVRLRTLEAAGRLRIPFTTGLLVGIGETPEERVETLEAIRESHARWGHVQEVIIQ
                     NFRAKPHTPMASAPEPGTGDMLRTAAVARLVLGAEANIQIPPNLSAHDFPLYLDAGIN
                     DWGGISPLTIDYVNPEAPWPAISVLRAHTEAAGLRLRPRLPVYPEYLLRRPEFLPALV
                     RDRLHAAADGDGYAKEGPARYGHATTRAW"
     gene            1768..>2310
                     /locus_tag="VEW91_05610"
     CDS             1768..>2310
                     /locus_tag="VEW91_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYM91092.1"
                     /translation="MPPREPGSSLGGLLTQIAAPVARILDRALGGADVTAEEAEVLLR
                     VSGRELVATMAVADELRCETVGHIVTYVVNRNINFTNVCIKGCGFCAFSRDFREEEGY
                     FLPEEEIVRRAREARDLGATELCVQAGLPPKMDGDFYIRLTRVLKEAVPEIHIHGFSP
                     EEVLYGAVRSGRPIREYLAEL"
BASE COUNT          346 a          819 c          793 g          352 t
ORIGIN      
        1 gcccgttccc cgatgcccac ggcccgcaac cacatgggcg acggggggtc cgtcaacggc
       61 aagatctacg tgatcggtgg gcggttgagt ggggcgttta tcattgccat gccggggaat
      121 acggacctgg tgcaggagta cgatccggcg gcggacgcct gggcgaccaa gacgcccatg
      181 ccgacggcgc gcagcgggct caactcggct acgctgaacg gcatcatcta cgtggccggc
      241 ggcgaggtgc ggacgtatca gtacctcgcg gcgtttcggg ccttcgaagc ctacgatccg
      301 gcgagcaaca cctggtggca gctgcctccc atgccctcgc cgaggcacga gtgcgcgatg
      361 gccgcgctgg ggaaccgaat ccacattgtg ggcggggccg tgcaatcggc gatcgtcccg
      421 ccagtacagg gcgtgagctt cggcacgccg gcgcacgatg cctttgagat cgccctctga
      481 ccggaggtcg cgcaggctcg gactccggct ctgactctga caagatgacg accgtgtctc
      541 cccagcgcct cagtgccttc ctcgacccgg gccgcatcgc ccgcacgtcc gccgtggcga
      601 gggtgctgga ccggaccctg gacggggtgc cgctcaccag ggacgatgcc gtgctcctga
      661 tcacctgtcc cgaagaggac gttcctgcgg tctgtgccgc cgcgcgcgtc ctgcgcgccc
      721 gcggaaaggg ccgggtggtc agcttctcgc cgaaggtctt catcccgttg acgcggctgt
      781 gccgggacgc ctgcggatac tgcacgttcc ggaccgaccc ccgatccgac ccccgcctgt
      841 acatgacccc ggaggacgtg ctggcggtgg cccgggcggg ggagcggctg gggtgcacgg
      901 aggcgctgtt caccctcggc gagcggccgg agcagcggtt ccccgaggcg cgccggtggc
      961 ttgccgcacg cgggcaccgg agcacgctct cctatctgcg cgagatggcg gaccgtgtgc
     1021 tgcgggagac cgcgctgctg ccgcacctga atcccggcac gatgagccac gcggagatgg
     1081 ccgccctccg cgacgtcagc gcgtcgatgg gcctcatgct ggaaactgtg agcccgaggc
     1141 tgtgcagccc cggggggccg cacgagcacg cccccagcaa acatcctcgc gtgcgcctgc
     1201 gcacgctcga agccgccggc cggttgcgca tccccttcac gaccggcctg ctcgtgggaa
     1261 tcggggagac gcccgaggag cgcgtggaga ccctcgaggc catccgcgag tcgcacgccc
     1321 gatggggcca cgtccaggag gtcatcatcc agaactttcg cgccaaaccc cacacgccga
     1381 tggcgtccgc tcccgagccg gggacggggg acatgctgcg gacggccgcc gtggcccggc
     1441 tcgtgctcgg ggccgaggca aacatccaga tcccacccaa cctctcggcc cacgactttc
     1501 ccctgtatct cgacgccggc atcaacgact ggggcggcat ctcgccgctc accatcgatt
     1561 acgtcaaccc cgaagccccc tggccggcga tctccgtcct ccgggcgcac acagaggcgg
     1621 ccgggctcag gctccggccg cgcctccccg tgtaccccga gtatctcctc cgccgtcccg
     1681 agtttctccc cgcgctggtc cgcgaccggc tccatgcggc tgcggatggc gacgggtacg
     1741 ccaaggaggg tcccgcgcgc tatggacatg ccaccacgcg agcctggtag ttcgctcggc
     1801 gggttgctca cgcagatcgc ggcgccggtt gcccgcatcc tcgaccgggc cctagggggt
     1861 gccgacgtca cggccgaaga ggccgaggtc ctgctccggg tgtcggggcg ggagcttgtc
     1921 gccacgatgg cggtcgcgga cgagctccgg tgcgagaccg tgggccacat cgtcacgtac
     1981 gtggtcaacc gcaacatcaa cttcacgaac gtctgcatca aggggtgtgg cttttgcgcg
     2041 ttcagccggg acttccggga ggaagaaggc tatttcttgc ccgaggagga gatcgtccgg
     2101 cgcgcgcggg aagcgcggga cctcggcgcc accgagctct gcgtccaggc aggactgccg
     2161 cccaagatgg acggcgactt ctacatccgg ctgacccgcg tcctcaagga ggcggtcccg
     2221 gagatccata tccacgggtt ttcacccgag gaggtcctct acggagcggt ccggtcgggc
     2281 cggcccatcc gcgagtatct tgccgagctc
//
LOCUS       DASQGH010000286         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4106, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000286 DASQGH010000000
VERSION     DASQGH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4106"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VEW91_05615"
     CDS             <1..1355
                     /locus_tag="VEW91_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYM91093.1"
                     /translation="DTVEKYLGGHVQPFLITEADFAWAVTKFFDVTRKVGESLLEVED
                     EHQEPAESAAVGVADTYDASPVVRVVNTIIHDAVRIGATDIHIEPEADKTRIRVRVDG
                     LLLDKTVLPRSVSDGVTSRLKVLAGIDIAERIRPQDGRIQVNIEGRDYDLRIATMGTA
                     FGERVAMRLLSNRQVLVGLERLGLFPEQQETLQALLGRQHGMILVTGPTGSGKTTTLY
                     ASISYINDRVRNIMTIEDPIEYRLPGITQIPVREKSGITFGVGLRALLRQDPDVVMVG
                     EIRDAETASIAVHAALTGHLVLTTLHTNDAAGALVRLQDMGIEPYLLTSSVLAMVGQR
                     LIRILCTACKRRYEAREADLRILGLPVDGSPMMLSAGAGCPECATLRYKGRTGAFELM
                     VMSDAIRELVLQRRSAGAIKEAAVAAGMTTLRGAIIRKVVDGVTSIDELQRLMVVEAG
                     "
     gene            1358..>1629
                     /locus_tag="VEW91_05620"
     CDS             1358..>1629
                     /locus_tag="VEW91_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYM91094.1"
                     /translation="MVSFVNAVVARLLPPAPLSPSGRAGPLTPAWPWGRRTGSPADTA
                     MLTYDLAVLLGAGLPLLQALEVLAQQTTDAGMRGVLRNLSREIQEG"
BASE COUNT          260 a          542 c          580 g          247 t
ORIGIN      
        1 tggatacggt agagaagtac ctgggcggcc acgtgcagcc gttcctgatc accgaggccg
       61 atttcgcgtg ggccgtcacc aagttcttcg acgtcacgcg caaggtcggg gagagcctcc
      121 tcgaggtcga ggacgagcac caggagccgg cggagtccgc cgctgtggga gtggccgaca
      181 cctacgacgc ctccccggtg gtccgcgtcg tcaacacgat cattcacgat gcggtgcgga
      241 tcggcgccac ggacatccac atcgagccgg aggccgacaa gacccgcatc cgcgtccgcg
      301 tcgacgggct gctgctcgac aagaccgttc tccccaggtc ggtgtccgac ggggtcacgt
      361 cccggctcaa ggtgctggcc ggcatcgata tcgcggagcg catccgcccg caagacgggc
      421 gaatccaggt aaacatcgag gggcgggact acgacctgcg gatcgccacg atgggcaccg
      481 ccttcggcga gcgggtggcg atgcggctcc tgagcaaccg gcaggtcctg gtcggcctgg
      541 agcggttggg cctctttccc gagcagcagg agacgctcca ggcgctcctc ggccgccagc
      601 acgggatgat cctcgtcacg ggcccgaccg gcagcggaaa gacgaccacg ctgtacgcct
      661 cgatcagcta catcaacgat cgggtccgca acatcatgac gatcgaggac ccgatcgagt
      721 accgcctccc cgggatcacc cagatcccgg tgcgcgagaa atcggggatc acgttcgggg
      781 tggggctccg cgccctcctt cggcaggatc cggatgtggt gatggtcggg gagatccgcg
      841 atgccgagac ggcgtcgatc gccgtccacg cggcgctcac cggtcacctg gtgctgacga
      901 ccctgcacac caacgacgcc gcgggcgcgc tggtgcggct gcaggacatg gggatcgagc
      961 cctacctctt gacctcctcg gtgctcgcga tggtgggcca gcggttgatc cgcatccttt
     1021 gcacggcgtg caaacgccgg tacgaggccc gggaggcgga tctccggatc ctcggccttc
     1081 cggtcgacgg atctccgatg atgctgtcgg cgggagccgg atgcccggaa tgcgccaccc
     1141 tgcgctacaa agggcggacc ggcgcgttcg agctcatggt catgtcggac gcgatccggg
     1201 agctggtgct ccagcgcagg tccgcgggcg ccatcaagga ggcggccgtg gccgcgggga
     1261 tgacgacgct ccgcggggcg atcatccgga aggtcgtgga cggcgtgacg agcatcgatg
     1321 aactgcaacg cctgatggtc gtggaggccg ggtagccatg gtgtccttcg tcaacgccgt
     1381 ggtcgcccgg ttgttgcctc cggctccgct ctccccgagc ggtcgggcgg ggccgctcac
     1441 cccggcctgg ccgtggggta ggcggaccgg aagcccggcg gatacggcaa tgctgacgta
     1501 cgacctggcg gtgctgctgg gggccggcct gccgctcctg caggcgctcg aggtcctcgc
     1561 ccagcagacc accgacgcgg ggatgcgggg cgtcctccgc aacctgtccc gggagatcca
     1621 ggaaggcag
//
LOCUS       DASQGH010000287         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_26721, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000287 DASQGH010000000
VERSION     DASQGH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26721"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VEW91_05625"
     CDS             <1..624
                     /locus_tag="VEW91_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HYM91095.1"
                     /translation="EFQTNDVAIFLDYLQKIPLDEYVEDWKARNDLISTALAEMSLEL
                     NIPIFAISPLDKEGCRLDERPAEADAEYSQFERPTMHHSMGSGDLEYDLDVAMVMAKD
                     WKATGELQQFLETRAKSANVDPDTIPQIDIVNLFVDKNRDAPASASYIVQYAFFVTLN
                     KFVELDYKLEKEYRGDYHGFAKLQQIYAYLLDTGLLTDAEQPAVARG"
     gene            638..>2030
                     /locus_tag="VEW91_05630"
     CDS             638..>2030
                     /locus_tag="VEW91_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYM91096.1"
                     /translation="MATTSRKKKLLGEVLVESGLITQDQLREAIALQKQSKERLGRVL
                     LGMGIATEKDIAMAIAKQLGLQFVDLDDVLPEEQALLALPEHLARRHHVLPLGAEDGK
                     LRVGMVDPLDVLALDDVRRHTGQEIDPVVISHDGFLRILNQYPALDDSVKQIIQDIKS
                     EVVEGEELENLRKLSDDAPVVRLVNVIVLQAIRQRASDIHIEPEETRVRVRYRIDGTL
                     YVSMTPPRHIHNAIISRVKIMAEMDIAERRLPQDGRIQLKVEGRDIDLRVSTIPTVFG
                     EKVVLRILDKSQTVVGIEKVGLLAENRLRFESMLTKPYGIILLTGPTGSGKTTTLYTM
                     LNKLNSTESNIITVEDPVEYQLAGINQVQVNNKAGLTFANGLRSFLRQDPDIIMVGEI
                     RDEETARIAIHAALTGHLVLSTLHTNDSAGAITRLVDMGIEPFLAASSLVGVIAQRLV
                     RVLCDKCKQPYTPP"
BASE COUNT          413 a          681 c          636 g          300 t
ORIGIN      
        1 gaatttcaga ccaacgacgt ggccatcttc ctcgactacc tgcagaagat ccccctcgat
       61 gaatacgtcg aggactggaa ggcgcggaac gacctcatct ccaccgccct ggccgagatg
      121 agcctcgagc tcaacatccc gatcttcgcg atctcccccc tcgacaagga ggggtgccgc
      181 ctcgacgagc gcccggcgga ggcagacgcg gagtactccc agttcgagcg ccccacgatg
      241 caccacagca tggggagcgg cgacctcgag tacgacctgg atgtggcgat ggtgatggcc
      301 aaggattgga aggccacggg ggagctccaa cagttcctgg agacccgggc caagagcgcg
      361 aacgtcgacc ccgacaccat cccgcagatc gacatcgtca acctcttcgt cgacaagaac
      421 cgggatgcgc ccgccagcgc gtcgtacatc gtgcagtacg cgttctttgt cacgctcaac
      481 aagttcgtgg agctggacta caagctggag aaggagtatc gcggggacta ccacggcttc
      541 gcgaagctcc agcagatcta cgcgtacctg ctcgacacgg gactgctgac ggacgccgag
      601 cagcccgcgg tggcgcgagg gtaggcggag ggcgactgtg gccacgacgt cgcggaagaa
      661 aaaactcctg ggcgaggtgc tcgtcgaatc cgggctgatc acccaggacc agctgcgcga
      721 ggccatcgcg ctgcagaagc agagcaagga gcgcttgggc cgcgtgctgc tcggcatggg
      781 gatcgccacc gaaaaggaca tcgcgatggc gatcgccaaa caactcggcc tccaattcgt
      841 cgatctcgac gatgtcctcc ccgaagaaca ggcgctcctc gccctccccg agcaccttgc
      901 ccggcggcac cacgtgctgc ccctcggggc cgaggacggc aagctcaggg tggggatggt
      961 ggatcccctg gacgtgctgg cgctcgacga cgtgcgccgc cacaccgggc aggaaatcga
     1021 tccggtggtg atctcgcacg atggcttcct ccggatcctc aaccagtacc cggcgctgga
     1081 cgactcggtc aagcagatca tccaggacat caagagcgag gttgtggagg gagaggagct
     1141 cgaaaacctc cgcaagctct ccgacgatgc cccggtcgtc cgcctcgtga acgtgatcgt
     1201 cctccaggcc atccgccagc gggccagcga catccacatc gagccggagg agacccgggt
     1261 gcgggtccgg taccgcatcg acgggaccct gtatgtgtcc atgacgccgc cccggcacat
     1321 ccacaacgcc atcatctccc gggtgaagat catggcggag atggacatcg ccgagcggcg
     1381 actcccgcag gacggccgga tccagctgaa ggtcgaaggc cgcgacatcg acctccgcgt
     1441 cagcaccatc cccacggtgt ttggcgagaa ggtcgtcctg cggatcctgg acaagagcca
     1501 gacggtcgtc ggcatcgaga aggtcgggct cctggccgag aaccgcctgc ggttcgagtc
     1561 gatgctcacg aagccctacg ggatcatcct gctgaccggg ccgaccggca gcgggaagac
     1621 caccacgctg tacacgatgc tgaacaagct caactccacg gagagcaaca tcatcacggt
     1681 cgaggatccc gtggaatacc agctcgccgg gatcaaccag gtccaggtca acaacaaggc
     1741 ggggctcacg ttcgcgaacg gcctccgatc gtttctgcgg caggacccag acatcatcat
     1801 ggtcggcgag atccgcgacg aggagaccgc ccgcatcgcg atccacgcgg cgctgaccgg
     1861 ccacctggtg ctgagcacgt tgcacaccaa cgattccgcg ggcgcgatca cccggctcgt
     1921 cgacatgggg atcgagccgt tcctcgccgc ctcatccctc gtcggcgtga tcgcccagcg
     1981 cctggtccgg gtcctgtgtg acaagtgcaa gcagccgtac accccgccgg
//
LOCUS       DASQGH010000288         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46618, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000288 DASQGH010000000
VERSION     DASQGH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46618"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VEW91_05635"
     CDS             complement(<1..571)
                     /locus_tag="VEW91_05635"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYM91097.1"
                     /translation="MPAGAKFCAQCGIPLAGDAQAHAEAGPILTAEARKVITAVFADL
                     VGSTPLTERLDPEEARQVVGAFYDEVRHAVERYDGTVANLLGDAVLAVFGLPASHEDD
                     PERAVRAGLALRDAMPALNDHLETRYGVGLALRVGINTGEVVAASGSTFDRDFLVSDA
                     VTTAARLQQTIAPGLVVVGERTHRLTRDAI"
     gene            702..1526
                     /locus_tag="VEW91_05640"
     CDS             702..1526
                     /locus_tag="VEW91_05640"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HYM91098.1"
                     /translation="MVSNWTAPGVIPGRRPSARWRDRTRLRLRRIRWARNRLGNGGAG
                     LSPWLYLLLAHLLADFVFQPYELVRLKERPIGLGIHAAIYGFLTAIIAVPLFPRWWIV
                     VPVLAAVHYGIDAVKGKVGHADGPASFVVFLGDQAAHLGALAAGVALAGVPVGAEISF
                     GPPAVTAALYYAVPYIATTFAGAIVVYQLALAYHTRSSPGDLLSLRLRAAGYAERGLA
                     LTAVLFLAPMFWSIAALWYGVRLIASHHRPGRWVEIGGSLAMVLVLGWFFRQGVGR"
BASE COUNT          245 a          577 c          631 g          319 t
ORIGIN      
        1 cgatggcgtc ccgtgtcagg cggtgggtgc gctcgccgac caccacgagg ccgggcgcga
       61 tcgtctgctg gagacgggcc gccgtggtga cggcatcgga gaccaggaag tcgcggtcga
      121 aggtcgatcc cgaggcggcc acgacctcgc cggtgttgat gcccacccgg agagccagcc
      181 ccacgccata ccgggtctcc aggtgatcgt tcagcgccgg catcgcgtcc cgcagcgcca
      241 gcccggcccg cacggcgcgc tcgggatcat cttcgtggga cgcggggagc ccgaagacgg
      301 ccagcacggc gtcgccgagg aggttcgcca ccgtgccatc gtaccgttcg acggcatgac
      361 ggacctcgtc gtagaatgcg ccgacgactt ggcgcgcctc ttccgggtcg agccgttcgg
      421 tcaggggggt cgagccgacg aggtcggcga acacggccgt gatgaccttg cgggcttcgg
      481 cggtgaggat cggtccggcc tcggcgtggg cttgcgcgtc cccggcgagc gggatgccgc
      541 actgcgcgca gaatttcgcg cctgccggca cctctgcgcc acactcgggg cacgtctgcc
      601 gttgaggttt gccgcaattg gcgcagaacc gtgcgcccgc gggattcccg tgaccgcagt
      661 tccagcaggt caccgtctca gtggacatgt gccgacaccg tatggtgagc aattggacgg
      721 ctcccggtgt gattcctggc cgccggccct cggcccgatg gcgcgacagg acacgcctcc
      781 gcctgcggcg cataagatgg gccaggaatc gtctcgggaa tggaggagcg ggtctgtctc
      841 catggctgta tctgttactc gcgcatctgc tggccgactt cgtgttccag ccttacgaac
      901 tcgtcaggct caaggagcgt cccatcggcc tgggcattca tgccgccatc tacgggttcc
      961 tcaccgcgat cattgccgtg ccgctttttc cccgatggtg gatcgtcgtg cccgtgctgg
     1021 cggccgttca ctatggcatt gatgccgtga agggcaaggt cggccacgcc gatgggccag
     1081 cctcgtttgt ggtgttcctg ggtgaccagg cagcgcacct cggggcattg gccgctgggg
     1141 tcgcgctggc aggggtgcca gtcggtgcgg agatctcgtt tgggccgccc gcggtgaccg
     1201 ccgccctcta ctacgccgtg ccgtacattg ctacgacgtt cgctggcgcg atcgtcgtgt
     1261 atcaactcgc tctagcctat catacccggt cgagtcccgg cgacctgctg agcctgcgcc
     1321 tccgggcggc ggggtacgcc gagcgtggcc tggcgctcac cgccgttctg tttcttgcgc
     1381 ccatgttctg gtcaatcgcg gcactgtggt acggcgtccg gttgatcgcc tcccaccacc
     1441 gcccggggag gtgggtcgag atcggaggca gtctggcgat ggtgctcgtg ttggggtggt
     1501 tcttccggca gggggtcggc cgatgagcct ccagatcgcc ggaccggcca gcgagcgtcg
     1561 cgtggtcccc gtccttttcg cggacatgca tcatggcagg aggcgcgtgt ccgctttgcg
     1621 cgattgggcg cgccctggca tctgcggccg gccgaagagg cgatcgcccg attcaagggg
     1681 gcgacccagc gacccccccg gagcagagga tcgcggcggg gagaaggctc gcatcacagt
     1741 ggtggaggga gcagatgatg agcgacagga ag
//
LOCUS       DASQGH010000289         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55010, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000289 DASQGH010000000
VERSION     DASQGH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55010"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VEW91_05645"
     CDS             <1..806
                     /locus_tag="VEW91_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYM91099.1"
                     /translation="IARHAKGWALDRIAPLDRNVMRVALYEIEHSDDVPTEVAIDEAV
                     ELIHTYSLIHDDLPAMDDSPTRRGRPTCHVLYGDAIAVLAGDALHALAFELLAKNAEV
                     PGVPSGHVVRAIAEVTQGIGTRGMVGGQVLDLLATRGSAATFDVREIHRLKTGSLIQA
                     CVRIGGILGGAAPGDLEALGRYGEHLGLAFQIVDDVLDVVGEDAKLGKGTGQDAAQAK
                     VTFPSVFGLAESRRLAQEATLQAVLALEPLGARGRWLGDLAAFLQGRER"
     gene            818..1642
                     /locus_tag="VEW91_05650"
     CDS             818..1642
                     /locus_tag="VEW91_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HYM91100.1"
                     /translation="MSLAGGRLDQWLVDRGHAATRSEAQAIIMAGLVTIDGRLADKPG
                     RRVTPKMVVEVGPQDARYVSRGGVKLAHALATFGIDVRGKVAVDLGASTGGFTECLLR
                     AGASRVVAVDVGRGQLAWRLRTDPRVVCLEGVNARYLTLAHTGGPCDLVTVDLAFISL
                     GLVWRPIAGVLAPEGSVVALIKPQFEAGREKVGRGGVVRDPAVHREVLGAVLEAARAS
                     GLVPLGITPSPLAGPAGNLEYLVYLRRGTGAGLAPEALDAAVAAAHARDGKPRGRP"
     gene            1639..2511
                     /locus_tag="VEW91_05655"
     CDS             1639..2511
                     /locus_tag="VEW91_05655"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYM91101.1"
                     /translation="MITAIGLAVNVEKLRGTPEVARLAREAVHLLAERGVQVMINRES
                     AEPLGHRALGVDESELVSRASVVVVFGGDGTILRAARAAAPHGIPILGVNLGGFGFLA
                     EVTGPEVEHALHRLLEGDYRLDERMMLRAWVDREGRQIQEFLALNDIVVTKSGYARLL
                     KLRTFVNAEHLATHLADGLIVATPTGSTAYSLSAGGPIVHPGVEGIVLTPICAHTLNA
                     RAVVVSAGDTIGIQVEPTGAPPILTVDGQEGFPLQAGDEVRVERSPHRTRLVRLGRGG
                     FYSLLRAKLTWGER"
     gene            2564..>2835
                     /locus_tag="VEW91_05660"
     CDS             2564..>2835
                     /locus_tag="VEW91_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYM91102.1"
                     /translation="MTQQRSKYIFVTGGVASALGKGITCASLGRLLRSRGWRVTLLKF
                     DPYVNVDAGTMNPFQHGEVFVTDDGAETDMDLGRYERFIDEILSRD"
BASE COUNT          392 a          935 c         1102 g          406 t
ORIGIN      
        1 cgatcgcccg ccacgccaag ggctgggcgc tcgaccgcat cgccccgctg gaccgcaacg
       61 tgatgcgggt ggcgctgtac gagatcgagc acagcgacga cgtgccgacc gaggtggcga
      121 tcgacgaggc ggtcgagctg attcatacct actcgctgat ccacgacgac ctcccggcga
      181 tggacgactc gccgacccgg cggggtcggc ccacctgcca cgtcctctac ggggatgcga
      241 tcgcggtgct ggccggggac gcgctgcacg cgctggcgtt cgagttgctg gccaagaacg
      301 cggaggtccc cggggttcca tcgggccatg tggtgcgggc gattgcggag gtcacgcagg
      361 ggatcggcac gcgggggatg gtcggcgggc aggtcctcga cctcctcgcc acgcgcggat
      421 ccgccgcgac cttcgatgtt cgcgagatcc accggcttaa gaccggatcg ctcatccagg
      481 cctgcgtccg gatcggaggg atcctcgggg gcgcggcccc gggagacctg gaggcgctgg
      541 gccggtacgg cgagcacctc gggctggcgt tccagatcgt cgatgacgtg ctcgatgtgg
      601 tcggtgagga tgccaaactc ggcaagggca cggggcagga cgccgcgcag gcgaaggtga
      661 cgtttccgtc cgtgttcggc ctggccgagt cccggcggtt ggcgcaggag gcgaccctcc
      721 aagcggtcct ggccctcgag ccgctggggg cgcgcgggcg gtggctgggg gatctggctg
      781 ccttcctgca gggccgcgag cgatgaggcc gggcacggtg tccctcgcag gcggccggct
      841 cgatcagtgg ctggtcgatc gcgggcacgc cgcgacccgg agcgaggccc aggcgatcat
      901 catggccggg ctggtcacca tcgacgggcg cttggccgac aagccgggac ggcgggtgac
      961 gcccaagatg gtggtggagg tcggcccgca ggacgcgcgg tacgtgagcc gggggggcgt
     1021 gaagctcgcc cacgcgctcg ccacgttcgg gatcgacgtg cggggcaaag tggcggtgga
     1081 cctgggggcg agcacggggg ggttcaccga gtgcctgctg cgcgcgggtg cctctcgggt
     1141 ggtcgcggtg gatgtcgggc gcggacaact ggcctggcgc ctccgcaccg atcctcgggt
     1201 cgtctgcctc gagggggtca acgcgcgcta cctgaccctg gcgcacacgg gcgggccgtg
     1261 cgacctcgta accgtagacc tcgcgtttat cagcctcggg ctggtctggc ggccgatcgc
     1321 gggggtgctc gccccggagg gctctgtggt cgcgcttatc aagccgcagt tcgaggcggg
     1381 ccgggagaag gtggggcgcg gcggggtggt gcgggatccg gccgtccacc gggaggtgct
     1441 gggtgcggtg ctcgaggccg cgcgcgcctc cggcctggtc cccctgggaa tcacgccctc
     1501 gcccctcgcg gggcccgccg gaaacctgga atacctcgtg tacctgcgcc ggggcaccgg
     1561 agcagggctc gctcccgaag cgctcgacgc ggcggtggcg gcggcccacg cccgcgacgg
     1621 caagccccga gggcggccgt gatcaccgcc atcggcctcg ccgtcaacgt cgagaagctc
     1681 cggggcaccc ccgaggtggc gcggctggcg cgggaggcgg tccacctcct ggccgagcgg
     1741 ggcgtgcagg tgatgatcaa ccgggagagc gccgagcccc tggggcaccg cgcgctgggg
     1801 gtcgacgagt ccgaactggt gtcgcgggcg agcgtggtcg tggtgtttgg cggggatggg
     1861 acgatcctca gggccgcccg ggctgcggcg ccccacggga tcccgatcct cggggtcaac
     1921 ctgggggggt tcgggttcct ggccgaggtg accggcccgg aggtcgagca cgcgctccac
     1981 cggctcctgg agggcgacta ccgtctggac gagcgcatga tgctgcgcgc ctgggtggac
     2041 cgcgagggcc ggcagatcca ggagttcctg gccctcaacg acatcgtggt caccaagagc
     2101 gggtacgccc ggctcctgaa actccgcacc ttcgtgaacg ccgagcatct ggccacccac
     2161 ctggcggacg gattgatcgt cgcgacgccc acgggatcca ccgcctactc gctctcggcc
     2221 ggcggcccga tcgtccaccc gggggtcgag gggatcgtgc tcacgccgat ctgcgcgcac
     2281 acgctcaacg cccgggcggt ggtggtgtcc gcgggcgata cgatcgggat ccaggtggag
     2341 ccgaccggcg cgccccccat cctgaccgtg gatgggcagg aggggttccc cctccaggcg
     2401 ggtgacgagg tgcgggtcga gcgctccccc caccggacgc gcctcgtgcg attgggccgc
     2461 gggggattct acagcctcct gcgcgccaaa ctgacctggg gagaacggta gccgatgcga
     2521 gacgagccga tcgaggcgca agccgcatag gagggcgggg tcgatgacgc agcagcggtc
     2581 caagtacatc tttgtcaccg ggggcgtcgc ctccgcgctg gggaagggga tcacctgcgc
     2641 ctcgctgggg cgcctgctgc ggagccgtgg gtggcgggtc acgctcttga agttcgatcc
     2701 ctacgtgaac gtcgacgccg gcacgatgaa cccgttccag cacggcgagg tgtttgtcac
     2761 cgacgacggc gcggagacgg acatggatct cggccgctac gagcggttca tcgacgaaat
     2821 cctcagccgg gacaa
//
LOCUS       DASQGH010000290         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38173, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000290 DASQGH010000000
VERSION     DASQGH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38173"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VEW91_05665"
     CDS             complement(<1..331)
                     /locus_tag="VEW91_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYM91103.1"
                     /translation="MIVLGLPRGGVVVAKEVAHLLHEPLDLVVVRKVGAPGIEELAIG
                     AVGETGTPILNYTMIKSNGIPAAYLTEAIEAALQEVWRRVKAYRVGPPPDVRHKTVVL
                     VDDGIATG"
     gene            1230..1631
                     /locus_tag="VEW91_05670"
     CDS             1230..1631
                     /locus_tag="VEW91_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYM91104.1"
                     /translation="MGTGEPWQAAAGEERAVVRIVARGLTGRVKLYNALRGYGFVVSP
                     DAPGDVFFHRSDCRIDPSTLEPGAEVAFDLVEMANGQVKAVRVGAPQGRRAQGEPAAP
                     GDDAGPGGSRPAFRQRVKETAAPLASQQPPL"
     gene            1709..>2302
                     /locus_tag="VEW91_05675"
     CDS             1709..>2302
                     /locus_tag="VEW91_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM91105.1"
                     /translation="MAPLTPELRDLMAHGRRRGYVTHDEILDRCPEADEDDDVFDRIQ
                     RTLSREGIEVVAEAPDRRKQPQGTEAREDELEGASIEDPVRMYLREIGKIPLLTPADE
                     VALAQRVEKGDAEAKRRLAEANLRLVVSIAKRYAGRGLPLLDLIQEGNRGLIRSVEKF
                     DWRLGYRFSTYATWWIRQHITRALADQSRTIRIPVNLG"
BASE COUNT          400 a          783 c          736 g          383 t
ORIGIN      
        1 acccggtcgc gatgccgtca tcgacgagca cgaccgtctt gtgacgaaca tcgggcggag
       61 gccctacgcg ataggctttg acgcggcgcc agacctcttg cagcgcggct tcaatggcct
      121 cggtgaggta cgcagcggga atcccgttac tcttgatcat ggtgtaattc agaatcggtg
      181 tcccggtctc ccccacggcc ccgatcgcga gctcctcaat gcccggcgcg cccacctttc
      241 ggaccacgac gaggtcgaga ggctcgtgga ggaggtgggc gacttccttg gcgacgacca
      301 ccccgcctcg cggcaggccc aggacgatca catcctcagc cttactgagc cgatttctaa
      361 cggcaacagc ctgttgcttc cccgcgtctt cgcggtcgac gaatctcacc actgacctgc
      421 ctttgcctcg gaaaaggctc gaacctgcgt ttggttacgc tcccccggtg ctcgccatca
      481 ggccgtaacc cgatcgtgcg cctcccgagc ggcagccttc cattcagtga gcaaggcttg
      541 ccttcgttct cggacccggc aggccgcttc ctcaagccta cgtaccgcca gcagaatgcc
      601 atcctgcaga tccttgacgt cgccgccctg atcgccgatc cgccgtgccg ctacccgcgc
      661 ggcgcctgcc gcattcagga gctgatgctc cgcactgatc agcccctcaa aggctcgcgc
      721 atggggagtc acgatggccg cccggtacgg ccctcgccct tctgtgttct gccgcattct
      781 ctaaccacct actccggagg ggaccacatc gctggcgaac cgaccctccg ctctcaatag
      841 tccgaacaca ctctgactgt actgcagcaa atcacgtacg cccatccgtc gtgggcccta
      901 ttgccggtcc cacctcaggg agcccgtata tccgacctcc acccgatgcg cagcccccaa
      961 atctgcgcta ttgatattta tgaggggttt tcttctacgc gatcctctac ggggggctta
     1021 ctgtggcgca agaacggcat gaatggagtg aacctgtaga agcgcgtccc ccacgcgggg
     1081 cggtgggacg gtccgagaga gatgatctca cggcccggct cgagcgcctt gaggccgttg
     1141 tgacggccat cgccgaacac ctgggcgtcc gacccggccg catctcggga ccccgccgcg
     1201 gcccagcgcg acagcccgcc ggacgccggg tggggaccgg cgagccgtgg caggccgcgg
     1261 ccggagagga gcgtgcggtg gtgcgcatcg tcgcccgcgg ccttacgggc cgtgtcaagc
     1321 tatacaacgc gctcagaggg tacggatttg tcgtgagccc agatgcgccg ggcgatgtgt
     1381 tttttcaccg cagcgactgc cggatcgatc cgtccacgct ggagccgggg gcggaggtgg
     1441 cgttcgatct tgtcgagatg gcgaacggcc aggtgaaagc cgtccgtgtg ggcgcccccc
     1501 aaggccggcg cgcccaagga gagccggcgg ctcccggaga cgatgccggc ccagggggta
     1561 gccggcccgc gtttcggcag cgtgtcaagg aaacagcggc acccctagcg tcgcaacagc
     1621 caccgctatg aaccgaggag aggccggagt gtctgggaag aagcgcacca cccccacgca
     1681 ggtgggtcag acgcctcggc ccactgaact ggcgcccctg acgccggagt tgcgggactt
     1741 gatggcgcat ggccgccggc gcggctacgt cacccacgac gaaatcctgg accggtgccc
     1801 cgaggcggac gaggacgacg atgtattcga ccggatccaa cgcaccctga gccgggaggg
     1861 gatcgaggtc gtggccgagg ctccggatcg tcggaaacag ccccagggca ccgaggcgag
     1921 ggaggacgag ctcgaggggg cctccatcga agaccctgtc cggatgtacc ttcgggagat
     1981 cggcaagatc cccttgctga cccccgcaga cgaggtcgcg cttgcgcagc gggtggagaa
     2041 aggcgacgcc gaggcgaaac gccgcctcgc cgaggccaac ctgcggctgg tggtctccat
     2101 cgccaagagg tacgcggggc gtggtcttcc gctgctggac ctgatccagg aaggaaaccg
     2161 cggcctgatc cggtctgtcg agaagtttga ctggcgcctg ggataccggt tcagcactta
     2221 tgccacgtgg tggatccggc aacatatcac tcgggccctc gccgatcagt cgcggacgat
     2281 tcgcatcccc gtcaatctcg gc
//
LOCUS       DASQGH010000291         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12644, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000291 DASQGH010000000
VERSION     DASQGH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12644"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VEW91_05680"
     CDS             complement(<1..353)
                     /locus_tag="VEW91_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HYM91106.1"
                     /translation="MSPAARIARRAARCAAPLLLAWAVLAGAAWAATLDDQVYAIARQ
                     LMCPVCAGQTVAESDAAVASEMKAIIREKLQAGETPDQILRYFVGQFGDSVLAEPHSG
                     GVALILYGAPPLALIV"
     gene            complement(337..672)
                     /locus_tag="VEW91_05685"
     CDS             complement(337..672)
                     /locus_tag="VEW91_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HYM91107.1"
                     /translation="MVNPKLRDPLADHLFRAVLRLRTVEECYRFFEDLCTIGEVKALA
                     QRFAVARMLDQGLTYEEIAARTGASSATISRVRRFLTYGADGYALVLKRLGNVGAGEG
                     RRRKREPRR"
     gene            complement(685..1767)
                     /gene="leuB"
                     /locus_tag="VEW91_05690"
     CDS             complement(685..1767)
                     /gene="leuB"
                     /locus_tag="VEW91_05690"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HYM91108.1"
                     /translation="MSAARARRIAVVPGDGIGREVIAEAVRVLSAVDRRYELGLEFEE
                     GLVGGAAIDAVGHPIPEETIALCRRSEAILLGACGGPKWDRGPRELRPEQALFTLRKT
                     FGLYANLRPATVPPALLTASPLRREIVEGADILIVRELTGGLYFGAQFRTGQGAEIAA
                     MDTLPYSAPEIRRVVRKACEIARDRPRHKVTSVDKANVLETSRLWREVATEVAREMPD
                     VTLEHMLVDNCALQLVCNPRQFDVIVTDNMFGDILSDEAAGVIGSLGLMPSASLGEQP
                     PGLFEPVHGTAPDIAGKGIANPLAAILTGALLLRHGLGHEPAATAIEAAVHRALQDGC
                     RTADLAGRGPALGTAAMTDAVLARLH"
     gene            complement(1767..>3266)
                     /gene="cimA"
                     /locus_tag="VEW91_05695"
     CDS             complement(1767..>3266)
                     /gene="cimA"
                     /locus_tag="VEW91_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HYM91109.1"
                     /translation="DKLRIAERLDEFGIDYIEGGWPGSNPKDLEFFEQARGRHWRHAR
                     ITAFGSTRRTGISAERDENLQVILAAGTPAAALVGKSWDLHVRQALRTSLDENLRMIE
                     ESVRYLKARGIEVVYDAEHYFDGWKANPEYALATLAAARRGGADVVVLCDTNGGCLPH
                     EIRAGVEAARVAAGDPLGIHTHNDGELAVANALVAVRSGAVHVQGTVNGLGERCGNAN
                     LISIIPNLQLKFGYDCIPASSLPRLGELSRFVSELANMAPNEYQPFVGKNAFAHKAGI
                     HVSAVMAHPPTYEHIPPEAVGNERRVVVSDLSGRGNVLYKAAEMGLDLSKDSPEVREI
                     LSQLKQHEHAGFQFEGAEASFELLVRRTLGQHRPAFTLRGLRVVVEKGPRGEAAAEAS
                     IRVAVDGQEEHTAAVGNGPVHALDQALRKALERFYPEIRQVRLVDYKVRVLNADAATA
                     AKVRVLIQSTDGERTWGTVGVSENVVEASWQALVDSLEYVLLRNHAGAR"
BASE COUNT          477 a         1202 c         1078 g          509 t
ORIGIN      
        1 acgatcaacg ccagcggagg cgccccgtac aggatcagcg ccaccccgcc tgagtgtggt
       61 tcggccagaa cgctgtctcc aaactggccg acgaagtatc ggaggatctg atccggggtc
      121 tcccctgcct gaagcttctc ccggatgatc gccttcatct cgctggcgac ggccgcatcg
      181 ctctcggcta ccgtctgccc cgcgcacacc gggcacatga gctgccgggc gatcgcatac
      241 acttgatcgt ccagggtcgc cgcccaggcc gccccggcga ggaccgccca cgccaggagg
      301 agcggggcgg cgcaccgggc cgcccgccgg gcgatcctag cggcggggct cacgcttccg
      361 ccttcgccct tcgccggctc ccacgttccc caggcgcttc agcaccaggg cgtacccatc
      421 ggcgccatag gtgagaaacc gccggacccg gctgatcgtt gccgaggacg ccccggtgcg
      481 agcggcgatc tcctcgtagg tcagcccctg atccagcatc ctggccacgg cgaagcgctg
      541 ggcaagggcc ttgacctcgc cgatggtgca cagatcctcg aaaaaccggt agcactcttc
      601 taccgtgcgg agccgcagga ccgcccggaa gaggtggtcc gccaacgggt cacggagttt
      661 ggggttgacc acgggaaccg ccgatcagtg gaggcgggcg aggacggcgt cggtcatcgc
      721 ggcggtgccg agcgcgggcc cccggccggc caggtcggcc gtccggcatc catcctgcag
      781 cgcgcggtgg acggccgcct cgatcgcggt ggcggccggc tcgtggccga gcccgtggcg
      841 gaggagcagt gccccggtca ggatcgccgc gaggggattc gcgatgccct tgccggcgat
      901 gtcgggggcg gtgccgtgca ccggctcgaa caaccccgga ggctgctcgc ccaggctggc
      961 cgacggcatg agcccgagcg acccgatcac ccctgcggct tcgtcgctga ggatgtcccc
     1021 aaacatgttg tcggtgacaa tgacgtcgaa ctgccgggga ttgcagacaa gctgcagggc
     1081 gcagttgtcc accagcatgt gctcgagggt cacatcgggc atctcccggg cgacctccgt
     1141 cgccacctcg cgccacagcc gggaggtctc caggacgttg gccttgtcca ccgaggtgac
     1201 cttatggcgg ggccggtcgc gggcaatttc gcacgccttg cgcacgacgc ggcggatctc
     1261 gggggccgag tacgggaggg tatccatcgc cgcgatctcc gctccctgcc cggtgcggaa
     1321 ctgcgcgccg aagtacaggc ccccggtcag ctctcgcacg atgaggatgt cggccccctc
     1381 cacgatctcc cgcctcagcg gagaagcggt caagagcgcc gggggcaccg ttgcggggcg
     1441 gaggttcgcg tacagtccga acgtcttccg gagggtaaac agcgcctgct cgggccggag
     1501 ctcccgcggg ccgcgatccc acttggggcc gccgcaggct ccgagcagga tggcctcgga
     1561 ccgccggcac agcgcaatcg tctcttcggg gatagggtga cccaccgcgt cgatcgccgc
     1621 gcccccgacc aggccctcct cgaactccag cccgagttcg tagcgccggt cgaccgcgct
     1681 caacacccgg acggcctcgg cgatcacttc ccggccgatg ccgtcgcccg ggacgacggc
     1741 gatccgccgg gcgcgtgcgg cgctcatcag cgcgctcccg cgtggttccg cagcagcacg
     1801 tactccaggc tgtcgaccag cgcctgccag gacgcctcga ccacgttttc ggacaccccg
     1861 accgtccccc acgtccgctc cccatccgtc gactggatca gcacccgcac cttggcggcc
     1921 gtcgcggcgt cggcgttcag gacgcgcacc ttgtagtcca ccaggcgcac ctgacggatc
     1981 tcggggtaga agcgttcgag cgccttgcgc aacgcctggt ccagcgcgtg caccggcccg
     2041 ttccccacgg cggcggtgtg ttcttcctgg ccgtccaccg ccacgcggat gctggcttcc
     2101 gccgccgcct ccccccgcgg gcctttctcg accacgaccc gcagcccccg gagcgtgaag
     2161 gcgggacgat gctgcccgag cgtccggcgg accagcaatt cgaacgacgc ctccgcgccc
     2221 tcgaactgga acccggcgtg ctcgtgctgc ttcaactgcg acaggatctc ccgaacctcc
     2281 gggctatcct tgctgaggtc cagacccatc tccgcggcct tgtagagcac gttgccccgg
     2341 ccggaaagat cgctgaccac cacccggcgc tcgttcccga ccgcctccgg ggggatgtgc
     2401 tcgtaggtgg gggggtgggc catcacggcg ctgacgtgaa tgcccgcctt gtgcgcgaac
     2461 gcgttcttcc ccacgaacgg ctggtattcg ttgggcgcca tgttcgccag ttcgctcaca
     2521 aaccgcgaca gctcacccaa ccgcgggagc gaggacgccg ggatgcagtc gtacccgaac
     2581 ttcagctgca ggttcggaat gatcgagatc aggttggcgt tcccgcagcg ctccccgagg
     2641 ccgttgacgg tcccctggac gtggacggct ccggagcgga cggcgaccag cgcgttggcg
     2701 acggcgagtt ccccgtcgtt atgggtgtgg atcccgagcg ggtccccggc cgcgacccgg
     2761 gccgcctcca cccccgcacg gatctcgtgg ggcaggcacc ccccgttcgt gtcgcagagg
     2821 acgacgacat ccgctccccc ccgccgcgcg gccgcgaggg tcgccagcgc gtactccggg
     2881 ttggccttcc atccatcaaa gtaatgctcg gcgtcgtaca ccacttcgat gccgcgggct
     2941 ttgaggtatc ggaccgactc ctcgatcatc cggaggttct cgtcgagcga ggtgcgcagc
     3001 gcttgccgca cgtgcaggtc ccacgatttc ccgaccagcg ccgcggcggg tgtgccggcc
     3061 gccagaatca cctgcaggtt ctcgtcccgt tccgcggaga ttccggtgcg acgggtgctc
     3121 ccaaacgccg tgatccgggc gtgccgccag tgcctccccc gggcctgctc aaagaactcc
     3181 aggtctttcg gattgctgcc gggccacccg ccttcgatat agtcgatgcc gaactcgtcc
     3241 agtcgctcgg cgatccggag cttgtc
//
LOCUS       DASQGH010000292         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4111, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000292 DASQGH010000000
VERSION     DASQGH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4111"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VEW91_05700"
     CDS             <1..248
                     /locus_tag="VEW91_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91110.1"
                     /translation="IHLERVSLVAGDSVEAAQEVASVENGKGAATAWLEPMRGEVPDA
                     ADESNGESWPAPRGLLGRLRSWWGGTRGRESARAGGR"
     gene            283..615
                     /gene="rplU"
                     /locus_tag="VEW91_05705"
     CDS             283..615
                     /gene="rplU"
                     /locus_tag="VEW91_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYM91111.1"
                     /translation="MYAVIETGGKQHRVKPGQVLEVDRLPQARGSEITFGRVLMLVDG
                     DQVTAGTPVVPGVRVAATVMAHPRGRKVLVGKFRSKKRYRRRVGHRQPLSRVRVDRIE
                     MEGASHGA"
     gene            605..865
                     /gene="rpmA"
                     /locus_tag="VEW91_05710"
     CDS             605..865
                     /gene="rpmA"
                     /locus_tag="VEW91_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYM91112.1"
                     /translation="MAHKKGGSSSRNGRDSNAHRLGIKRFAGERVIAGSVLVRQRGTL
                     YHPGANVGVGRDDTLFALASGVVRFDRRGRDRRLVSVQPAGA"
     gene            937..2019
                     /gene="obgE"
                     /locus_tag="VEW91_05715"
     CDS             937..2019
                     /gene="obgE"
                     /locus_tag="VEW91_05715"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYM91113.1"
                     /translation="MFIDLAHIQVKAGNGGNGCVAFRREKFVPKGGPSGGDGGRGGDV
                     ILVADPGLNTLLPFRYRRRFAAGRGAHGEGSGRAGRAGKDLMIPVPVGTTVHDEATGR
                     QLADLVSPGHRVTVAEGGRGGRGNAHFATPTRQAPRNAEPGGEGEERRVRLELRLLAD
                     VGLVGLPNAGKSLLLSRISAARPKVADYPFTTTEPVLGVVPLPNAPGIVVADIPGLIE
                     GAHRGAGLGHEFLRHIERTRVLVHVVDLAGDDPERQLATVERELALYAPPLDDRPTIV
                     AANKMDLPLARARWDAFAAYLASRGRTVLPISAATGEGVAALLRTVVDFLHATRPQGP
                     KESPSGGEKTSVGTAHDGTRRVRGQA"
     gene            2016..2660
                     /gene="nadD"
                     /locus_tag="VEW91_05720"
     CDS             2016..2660
                     /gene="nadD"
                     /locus_tag="VEW91_05720"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HYM91114.1"
                     /translation="MSRIGIMGGTFDPIHYGHLVTAGEARWQFGLSQVIFVPNRHPPH
                     KEPGEVSDPEDRYLMTILATVTNAHFTVSRIEIDRPGPSYAIDTIRELRRTQPEDDLY
                     YITGADALMQILHGEWRETEQLLGLCQFIAASRPGYQLDPAVWSSANHRLGERIRNVH
                     TMEIPAMAISSTDIRARVRDGRPTKYLLPEPVEEYIAKHRLYVAAPAGVRETGL"
     gene            2660..2998
                     /gene="rsfS"
                     /locus_tag="VEW91_05725"
     CDS             2660..2998
                     /gene="rsfS"
                     /locus_tag="VEW91_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYM91115.1"
                     /translation="MEARGKALLAAAAAEAKLAERVMVLDVQEHTPVTDFFVLASGTN
                     RIQIKAITDAVEDALGAVGERPGRAEGRDGDRWVLLDFGDVVIHIFAPSERAYYDLER
                     LWGDAAVVER"
     gene            3068..3142
                     /locus_tag="VEW91_05730"
     tRNA            3068..3142
                     /locus_tag="VEW91_05730"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3100..3102,aa:Ala,seq:ggc)
     gene            3177..>3293
                     /locus_tag="VEW91_05735"
     CDS             3177..>3293
                     /locus_tag="VEW91_05735"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HYM91116.1"
                     /translation="MVPVRRSTATFFARVYALVRRIPPGRVATYGAIARALGD"
BASE COUNT          503 a         1093 c         1207 g          490 t
ORIGIN      
        1 gcatccacct cgagcgggtg agcctggtcg ccggggattc ggtcgaggcg gcgcaggagg
       61 tcgcttcggt cgagaacggc aagggcgcgg ccaccgcctg gctggaaccg atgcgggggg
      121 aggtccccga cgcggccgac gagagcaacg gcgagtcctg gcccgcccca cgaggcctcc
      181 tcggacggtt gcgctcgtgg tgggggggaa cgcgcgggcg cgagagcgcg cgcgctggag
      241 gtcgttgaca ctccccgggt cccgtgctac catttcgtgg tcatgtacgc ggtgatcgaa
      301 accgggggaa agcagcatcg cgtgaaaccg gggcaggtgc tcgaggtgga ccgcctgccg
      361 caggcgaggg gatcggagat cacgtttggg cgcgtgctca tgctcgttga tggagaccag
      421 gtcactgcgg gaacaccggt ggtgcccggg gtccgcgtgg ccgcgaccgt gatggcgcac
      481 ccccgcggcc gcaaggtgct ggtggggaag ttccggtcga agaagcgcta ccggaggcgc
      541 gtgggacacc gccagcccct gagccgggtc cgcgtcgacc ggatcgagat ggagggggca
      601 tcccatggcg cataaaaagg gcgggagcag ttctcggaac ggacgggata gcaacgccca
      661 ccggctgggc atcaagcggt ttgcgggcga gcgcgtcatc gcggggagcg tgctcgtgcg
      721 gcagcgggga acgctttacc atcccggcgc gaacgtcggg gttggacgcg acgacaccct
      781 gttcgcgctg gcctcgggcg tcgtgcggtt cgatcgacgc ggccgcgacc gccggctggt
      841 ctcggtccag cctgccgggg cgtaggctcc ccggcctgcc ccccgcgggg gggtgtcgga
      901 cgcctcgggc ttggagcccg gggccttttt cttgagatgt ttatcgacct cgcgcacatc
      961 caggtcaaag ccggcaacgg cggaaacggc tgcgtagcgt ttcggcggga gaagtttgtc
     1021 ccgaagggcg ggccgtccgg cggggacggc gggcgtgggg gcgatgtgat cctcgttgcc
     1081 gatccgggtc tcaacaccct gctgcccttt cggtaccgcc gcaggttcgc cgccggccgc
     1141 ggcgcccacg gcgaggggag cggccgggct gggcgcgccg ggaaagatct gatgatcccc
     1201 gttcccgtcg ggaccaccgt ccatgacgag gccacgggcc ggcaactggc cgatctggtc
     1261 tccccgggcc accgggtgac cgttgccgag gggggccgcg ggggacgggg caacgcacac
     1321 ttcgccacgc ccacccgcca ggccccgcgc aacgcggaac ccggaggcga gggcgaggag
     1381 cggcgggtgc gcctcgaact ccgactcctc gccgatgtcg ggctcgtcgg tctgccaaac
     1441 gccggcaagt ccctgctcct gagccggatc tccgccgccc gcccgaaggt cgcggactac
     1501 ccctttacca ccacggagcc ggtgctggga gtggtccccc tccccaacgc accggggatc
     1561 gtggtcgccg acatccccgg gctcatcgag ggagcccacc gaggcgccgg gctcggtcac
     1621 gagttcctcc ggcacatcga gcgcacgcgg gtgctcgtgc acgtcgtcga tctcgccgga
     1681 gacgacccgg agcggcagct cgcgacggtg gaacgagaac tggcgttgta cgcgcctccc
     1741 ctcgacgacc gccccacgat tgtggccgcg aacaaaatgg acctcccgtt ggcgagagcg
     1801 agatgggacg cgtttgcggc ctacctcgcc tcccggggac ggacggtcct ccccatctcc
     1861 gccgccaccg gcgaaggggt cgcggcgctg ctccggaccg tggtggactt cctgcacgcc
     1921 acccgccccc agggaccgaa ggagagccct tccggggggg agaagacgag cgtggggaca
     1981 gcgcacgatg ggactcgacg ggtccggggg caggcgtgag taggatcggc atcatgggtg
     2041 ggacgttcga ccccatccac tacgggcatc tggtcaccgc cggcgaggcg cgctggcagt
     2101 tcgggctgtc ccaggtgatc tttgtgccca accgccatcc gccgcacaag gagcccggtg
     2161 aggtgtccga ccccgaggac cggtacctga tgacgatcct ggccacggtg acgaacgcgc
     2221 acttcacggt ctcccggatc gagatcgacc gcccgggccc gtcgtacgcc atcgatacga
     2281 tccgcgagct gcgccggaca cagcctgagg atgacctgta ctacatcacc ggcgccgacg
     2341 cgctcatgca gattctgcac ggcgagtggc gggagaccga acagctgctg gggctgtgcc
     2401 agttcatcgc cgcaagccgg cccgggtacc agctggaccc ggcggtctgg tcctcggcca
     2461 accaccggct gggcgagcgg atccggaacg tgcatacgat ggagattccc gcgatggcga
     2521 tcagcagcac ggatatccgg gcgcgtgtgc gcgacggccg cccgaccaag tacctcctgc
     2581 ccgaaccggt cgaagagtat atcgcgaagc accggctgta cgtggcagcg ccggccgggg
     2641 tgcgcgagac ggggttgtag tggaggcgag ggggaaggcg ctcctggcgg cagcggcagc
     2701 cgaggccaaa ctcgcggaac gcgtcatggt cctcgatgtc caggagcaca ccccggtgac
     2761 cgacttcttc gtcctggcca gcggcaccaa ccggattcag atcaaggcta tcaccgacgc
     2821 ggtcgaggac gcccttgggg cggtgggcga acggccgggc cgcgccgagg gccgcgacgg
     2881 ggatcgctgg gtgctcctgg actttggcga cgtggtcatc cacatcttcg ctccgtccga
     2941 gcgcgcgtac tacgatctgg agcggctgtg gggggatgcg gcggtcgtcg agcggtgaag
     3001 cctcgggggc ggcgatcgtt gttctcgccc gatgaatccg ctataatacg gtgtcgcggt
     3061 ttcacgtggg gctgtagctc agcgggagag cgcctctttg gcgtagagga ggccgtgggt
     3121 tcaattccca ccagctccac cactgctcgc ggactcttct tgcgttcacc ccgctgatcg
     3181 tgcctgtcag aagatctacg gcgaccttct tcgcccgcgt ctacgccctg gttcgccgaa
     3241 tcccaccggg ccgcgtcgcc acctacgggg cgatcgcgcg cgccctggga gat
//
LOCUS       DASQGH010000293         8454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32492, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000293 DASQGH010000000
VERSION     DASQGH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32492"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1071)
                     /locus_tag="VEW91_05740"
     CDS             complement(163..1071)
                     /locus_tag="VEW91_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYM91117.1"
                     /translation="MMTPPIPTPCTEAAQVNVSFAELGAWRIVVLGIVQGITELLPIS
                     STAHLRIIPSLLGWPDPGSAFSAAMQLASLLAVATFLGGDVRRITGRAVTAARRGDWK
                     DHSLRITVGILGGTLPIAAAGLVLRRTLEACGSPLREVAVIGWACVVMGVLLALAERY
                     GQRGREGAKRRWADLSVRDCIAVGLAQAFALVPGVSRSGSTLTTGLALGMERETAARF
                     SFLLGLPAIVLAGLVELLSLRKAGLGAGGWSLLALGLAAGSASAYLALWGLVRYLKEH
                     TSWVFVWYRIALGALLVWGAAAGRFR"
     gene            complement(1068..3341)
                     /locus_tag="VEW91_05745"
     CDS             complement(1068..3341)
                     /locus_tag="VEW91_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM91118.1"
                     /translation="MRYTGVPLRRREDRPLLVGAGRFIDDIRLPGMLHMAVVRSPQAH
                     AYLAGIAPDDALAAPGVVAVITAKDLPAPAPRIGVPPVFPGLDSVTHPLLADQAVRYA
                     GEPVAVVLARSRYEAEDAAERVRVRFEVLPPVMDLDAAIAPGAPVLHVGRTSNLVFAH
                     DAGGGDPGAAFRRAAVVVDLTMEQPRLAAVPLECRGAVAAHDRVADRTEVWLSTQTPH
                     VARAQIARILHVPPTRVRVVAPDVGGGFGSKGSLYPEEVLVAYLARRLGRPVKWVEDR
                     IENLKSTTHGRGQRARVRAAAAEDGTVLAVDADVLVDLGAYCLFATASIPARTPLVGL
                     GAYRIPNFRYRIRGVVTTKAPTGPYRGAGRPEAAYYIERVMDVLAARLGLDPAEIRRR
                     NFITTFPHTSPTGLTHDSGDYRALLDRALTLAGYARWRAEQARRRAAGGPPIGIGLST
                     WIEVAGGGELWESGAVRLDASGRVTVLTGSSPHGQGLETAFSQIAADALGIAPERVTV
                     LHGDTDAVPLGVGTFASRSLAIGGSAVFAAAAQLREQIVALAARRLEAAPQDIVLAGG
                     TATVRGAPGRAVALAELAKAAETADILEASVRFASERSMVPSGAHVAVVEVDREIGAV
                     KVLQYTAVDDCGRLINPLLVRGQAHGSVAQGLAQALFEQVVYGGEDGQLLTGTLLDYP
                     VPTAADLPAITWEYIESLSPLNPLGAKGAGESGTIGAPPAIANAVEDALRPSAAGRLA
                     LPLTPDRVWRASRGAQA"
     gene            3407..4657
                     /locus_tag="VEW91_05750"
     CDS             3407..4657
                     /locus_tag="VEW91_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91119.1"
                     /translation="MSRRMSGTKGPRVTRRALLTAALGGAAALAGGAAGGRGGTGQPF
                     RVPAVLAASTGPVKLGVLLPYSKVYQQLGEDITSAMTLYFESVGWTAGGRRITLIRED
                     EDIDPQVALRKARKLIEGDNVDLMTGLVSSASALAIRDLVHNTKTVLVISNAGANAVT
                     RARRSPYIFRISFSNWQTAYPIGSWFYENVARSCVVGGSDYAAGHEDINAFKENFLAH
                     EGRVLGEVYPPLNNTDYGPYIAQMQRMRPEAVFVFFAGSDAVRFVTQATQYGLLKEAR
                     LVGPGFLVEEDVLAAQGKNALGAYSCLHWALTLQKPENLAFTRAYRERWKREATVYAL
                     QGYDAARVIVQALNTTGGDTVNKTGLIEAIASVKFASPRGAVAFDPETHNVIQTVYLR
                     QVREVGQALHNVVFADLGVFRDPG"
     gene            complement(4668..5285)
                     /locus_tag="VEW91_05755"
     CDS             complement(4668..5285)
                     /locus_tag="VEW91_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYM91120.1"
                     /translation="MDGILYRSLHAAVRALARALFHLEVVGGEHVPKTGGAVIVANHE
                     SWLDPILLPLALPRKPAFLAMEELWRMPGLALVMRAYGPLAIPINRSGVDATALKRAL
                     RALEGGALLIVFPEGGITPVGQTRPFHRGAAMLAARTRVPMIPIGIQGTADALPLGRI
                     LPRRRPITVRIGAPIAPPGVEREDLARASDEAASRIRALLNASPA"
     gene            complement(5321..5782)
                     /locus_tag="VEW91_05760"
     CDS             complement(5321..5782)
                     /locus_tag="VEW91_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008011035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HYM91121.1"
                     /translation="MFKGTVLSIHITPAAAGNMISVKEVRAVPGKGLDGDRYFRQTGT
                     YSKTPGSGREITLIASEAIEALGRDYGITIEAGESRRNIVTRGVPLDHLVGREFTVGA
                     VRVRGVRLCEPCGHLEKLTREGVRAGLVHRGGLRAQILTEGTIRPGDPVEA"
     gene            5932..6690
                     /locus_tag="VEW91_05765"
     CDS             5932..6690
                     /locus_tag="VEW91_05765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM91122.1"
                     /translation="MGAANKVAIVTGAGTGIGRAVTMALAQDGYSVVLAGRRRERLDA
                     TARAVAASETRTLVVPTDVTDPASVRALFARTKEAFGRLDLLFNNAGIGAPPVPLEDL
                     PYEQWKAVVETNLSGAFLCTQEAFRLMKSQTPRGGRIINNGSISAHNPRPNSAPYTAT
                     KHAITGLTKSASLDGRKYDIACGQIDIGNAATEMTARMVEGVAQADGSIRPEPRMDPS
                     HVARAVVYMASLPLDTNVQFMTVMATKMPFVGRG"
     gene            complement(6747..7148)
                     /locus_tag="VEW91_05770"
     CDS             complement(6747..7148)
                     /locus_tag="VEW91_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HYM91123.1"
                     /translation="MAAFRAQGRSITAQRMAVFCALEHAEDHPTAEELHAHLRDTVPG
                     FAQKTVYAILHELADLRLVEPIPLPVGATRWEPNTTPHGHLFCDVCRRVMDLPVDPTV
                     LMPLVRRAGRGFDPRGASLVVHGRCARCAAS"
     gene            7402..8340
                     /locus_tag="VEW91_05775"
     CDS             7402..8340
                     /locus_tag="VEW91_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HYM91124.1"
                     /translation="MLLVIGVLVWQAVTAGGSPDPTAKHLSPAAAVVDTGILVFREGL
                     ESLLVLSAIAASLSRTHSRWGRPVATGAGIAFLTTIATWFVVVAILSDVNAPELDLQA
                     ATGLLAVLVLLVIMNWFFHRVYWTGWITHHTRTGRALVENPGTPSAVVVRGLVLLGFA
                     SVYREGFEVVLFLQSLRLQVGADVVFLGSLIGVGLTLLVAALNFLSHYRLPYRKMLVL
                     TGILLGGVLVVMVGETVQEMQQAHWLAATKLLHFDLPNWMNVWFSVYNSWESLTAQVL
                     TVVFVIGSYLVAEYVQIRRPRRQAVVLAQEALDTRS"
BASE COUNT         1301 a         2915 c         2993 g         1245 t
ORIGIN      
        1 tgacgctcgg gcacgggctc gggcggccgc agcggggggc gccggggcgg gcctcggagg
       61 cagcggggcg ggccatcgtc gagcggctgg gtcgcctgtc gccgacggtg gcaatcgaat
      121 ggcagcggga tggctttggg gccgtgcgcg gattctgatc ctctaccgga accgtcccgc
      181 cgcggcgccc cagacgagga gcgccccgag cgcgatccgg taccacacga agacccagct
      241 ggtgtgctct ttgaggtagc gcaccagccc ccacagcgcg agataggccg aggcgctgcc
      301 cgccgcgagc cccagcgcca gcagcgacca tcccccggcc cccaggccgg ctttgcggag
      361 gctgaggagt tccaccagcc cggccagcac gatcgccggg agcccgagca ggaaggagaa
      421 gcgggcagcc gtctcccgct ccatgcccag ggcgagacct gtggtcaggg tagatcccga
      481 ccgggacacc cccggcacca gcgcaaacgc ctgggccagg ccgacggcga tgcaatctcg
      541 gacgctcaga tccgcccacc gccgcttcgc gccctcgcgg ccccgctggc cgtagcgctc
      601 ggccagggcg agcagcacgc ccatgacgac gcacgcccat ccgatgaccg cgacctcccg
      661 cagcggcgac ccgcatgcct cgagggtccg ccggaggacg agccccgccg cggcgatggg
      721 cagggtgccg cccaggatcc ccacggtgat cctgagcgaa tgatccttcc agtctccccg
      781 ccgggccgcg gtcaccgcgc ggcccgtgat gcggcggacg tccccaccga ggaaggtggc
      841 gacggcaagc aggctcgcca gctgcatggc cgcggagaag gccgatccgg gatccggcca
      901 ccccagaagg ctgggaatga tcctgaggtg ggcggtgctg ctgatcggca ggagctcggt
      961 gatgccctgg acgatcccca gcaccacgat gcgccaggcc cccagctcgg cgaaactgac
     1021 gttgacctgc gccgcctcgg tgcagggggt ggggatcggc ggtgtcatca tgcctgggcc
     1081 cctcgggacg cccgccacac ccggtcgggc gtgagcggga gcgcgagccg tccggcggcg
     1141 gacgggcgca gcgcatcttc gaccgcgttc gcgatcgccg ggggtgcacc gatcgtgccc
     1201 gactcgccgg cgcccttggc ccccagcggg ttgagcgggg atagcgactc gatgtactcc
     1261 caggtgatcg cgggaaggtc cgccgccgtg gggaccgggt aatccaggag cgttccggtg
     1321 aggagctggc cgtcctcgcc gccgtacacc acctgctcga acagcgcctg agccagaccc
     1381 tgcgccacgc tgccgtgcgc ctgcccccga acgagcaggg ggttgatcag tcggccgcag
     1441 tcgtccaccg cggtgtactg cagcaccttc accgcgccga tctcccggtc cacctcgacg
     1501 accgcgacgt gcgcgcccga cgggaccatg gagcgctcgg atgcgaatcg aaccgaggcc
     1561 tccaggatgt ccgcggtctc cgctgctttg gcgagttcgg ccagcgccac cgcccgtccg
     1621 ggggcgcccc gcacggtggc ggtcccgccc gccaggacga tgtcttgcgg cgcagcttcc
     1681 aggcggcgcg cggcgagcgc gacgatctgc tcgcgcagct gggccgccgc cgcaaagacc
     1741 gcggagccgc cgatggcgag cgagcggctg gcgaacgttc ccacccccaa tggcacggcg
     1801 tcggtatccc catggagcac cgtcacgcgc tcgggggcga tcccaagggc gtccgctgcg
     1861 atctggctga atgccgtctc gagcccttgc ccgtggggag aggaaccggt gagcaccgtc
     1921 acccgccccg aggcgtcgag ccggaccgcc ccgctctccc agagttcccc cccgccggcc
     1981 acctcgatcc acgtgctgag tccgatcccg atgggggggc ctcccgcagc ccggcgtctg
     2041 gcctgttccg cccgccagcg tgcgtagccg gccagggtca gcgcacggtc gagcagggcc
     2101 cggtagtcgc cggaatcgtg ggtcaggcct gtcgggctcg tgtgcgggaa tgtggtgatg
     2161 aaattccggc gccggatctc ggccgggtcc agcccgagcc gcgccgcgag cacgtccatc
     2221 acccgctcga tgtagtaggc cgcttcgggt cggcccgccc cgcggtacgg gcccgtaggc
     2281 gccttggtcg tcacgacacc ccggatgcgg tagcggaagt tggggatgcg gtaggcgccg
     2341 agccccacca gcggcgtgcg ggccgggatc gatgcggtcg cgaagaggca gtacgcgccg
     2401 agatcgacga ggacatcggc atccaccgcg aggaccgttc catcctccgc cgcggcggcg
     2461 cgaacccgcg cccgctgccc gcggccgtgg gtcgtgctct tgaggttttc gatgcggtcc
     2521 tcgacccact tgacgggccg tccgagccgg cgcgccaggt aggccaccag cacctcctcc
     2581 gggtacaggc tgcctttcga cccgaacccg cccccgacat cgggggccac cacgcggacg
     2641 cgcgttgggg gaacgtgcag gatcctggcg atctgcgccc gcgccacgtg cggtgtttgg
     2701 gtggagagcc acacctctgt ccggtcggcg acacgatcgt gggcggcgac cgcgccccgg
     2761 cactccaacg ggaccgcggc cagcctgggc tgctccatcg tgagatcgac cacgaccgcc
     2821 gcccggcgga acgcggcgcc aggatcgccg ccgccggcgt cgtgtgcaaa gacgaggttg
     2881 gaggtccggc cgacgtgcag caccggggcc ccaggggcga tggccgcgtc gaggtccatg
     2941 acgggcggaa gcacctcgaa acgcacgcgg acgcgttcgg cggcgtcttc ggcctcatac
     3001 cggctgcggg ccaggacgac ggccacgggc tcgcccgcgt accggacggc ctggtcggcg
     3061 agcagcggat gggtcacgga gtccaggccg ggaaacacgg gcgggacgcc gatgcggggg
     3121 gcgggggccg gcaggtcttt ggcggtgatg accgcaacga cccccggcgc cgccagagcg
     3181 tcatcgggcg cgatcccggc gaggtaggcg tgcgcttgcg gcgaccggac caccgccatg
     3241 tgcagcattc ctggcaggcg gatatcgtcg ataaaccgcc cggcgccgac caagagcggg
     3301 cggtcctcgc gtctccgcag gggcaccccg gtataccgca tgcggaggag gttcgcccca
     3361 tcgtccgtcg aagcctctgc ggtaccctgg gaggaggagc gcggagatga gcaggcggat
     3421 gagcgggaca aaagggccac gggtcacgcg gcgggccctg ctgaccgcgg cactcggcgg
     3481 ggccgcggcg ctggcgggcg gcgccgcggg ggggcgcggg gggaccggtc agccgttcag
     3541 ggtgccggcg gtcctggccg cctcgaccgg accggtcaag ctcggggtgc tgctgccgta
     3601 ctcaaaggtc taccagcagc tcggcgagga catcacgagc gcgatgacgc tgtacttcga
     3661 gtccgtgggc tggacggccg ggggccggcg gatcaccctg atccgggagg atgaggacat
     3721 cgatccgcag gtcgccctgc gcaaggcccg caagctcatc gagggcgaca atgtcgatct
     3781 catgaccggg ctggtgagca gcgcgagcgc gctcgccatc cgcgacctcg tccacaacac
     3841 gaagaccgtg ctcgtcatct ccaacgccgg cgccaacgcg gtgacccggg cccggcgcag
     3901 cccctacatc ttccggatct cgttctcgaa ctggcagacc gcctacccga tcggcagctg
     3961 gttctatgaa aatgtggcca ggagttgcgt ggtgggggga tccgactacg cggccggcca
     4021 cgaagacatc aacgcgttca aggagaactt cctggcacac gagggccggg tgctcggaga
     4081 ggtgtatccg cccctcaaca atacggatta cgggccctac atcgcgcaga tgcagcggat
     4141 gcggccggag gcggtgtttg tcttcttcgc cggcagcgac gccgtccggt ttgtcaccca
     4201 ggccacgcag tatgggttat tgaaagaggc ccggctcgtg ggcccggggt tcctggtgga
     4261 agaggacgtc cttgcggcgc aggggaagaa cgcgctgggc gcctactcct gcctgcattg
     4321 ggcgctcacg ctgcagaaac cggagaacct cgcatttacc cgcgcctacc gggagcggtg
     4381 gaagcgcgag gcgacggtgt atgccctgca ggggtacgac gccgccaggg tgatcgtcca
     4441 ggcgctgaat acgaccggcg gggacacggt caacaagacg ggactgatcg aggcgatcgc
     4501 gtcggtgaag ttcgcgagcc cgcgcggcgc cgtggcgttt gaccccgaga cacacaacgt
     4561 gatccagacg gtctacctgc ggcaggttcg ggaggtcggg caggcgctgc acaacgtcgt
     4621 ctttgccgac ttgggcgtct tccgggatcc cggctaagcg ggcgccgtca cgccgggctc
     4681 gcattcagca gggcgcggat gcgtgaggcc gcttcgtcgc tcgcccgggc gagatcctcg
     4741 cgctccacgc ccggtggggc gatcggtgcg ccgatgcgga cggtgatggg acggcgccgc
     4801 ggcaggatcc ggccgagggg gagggcgtcc gccgttcctt ggatcccgat cgggatcatc
     4861 ggcacccgcg tgcgggcggc cagcatcgcc gccccccggt ggaacggccg ggtctggccc
     4921 acaggggtga tgccgccctc gggaaagacg atgaggagtg cgccgccctc gagcgcccga
     4981 agggcgcgtt tcaacgccgt cgcatcaacg ccgctccgat tgatggggat ggccagcggc
     5041 ccgtaggcgc gcatgacgag tgcgagcccc ggcatccgcc acaactcttc catggcaagg
     5101 aacgccggct ttcgcggcag ggcgagcggc aggaggatcg gatcgagcca actctcgtgg
     5161 ttggcgacga tcaccgcgcc cccggtcttg ggcacgtgct caccaccgac gacctcgagg
     5221 tggaacagcg cgcgggccag cgctcggacg gcggcgtgaa ggctgcggta gagaatcccg
     5281 tccagggaac gcgccctccg ggatcatcgg tccccgggcg tcaggcttcg accgggtctc
     5341 ccggtcggat ggttccctcg gtaagaatct gcgcccggag tcccccgcgg tggaccaggc
     5401 cggcccgcac cccttcccgc gtgagctttt cgaggtgccc gcacggctcg cacaggcgaa
     5461 ccccccgcac cctcacggcc ccgacggtaa actcccggcc gacgaggtgg tcgaggggca
     5521 ccccacgcgt gacgatgttc cggcggctct cgccggcctc gatggtgatg ccgtaatcac
     5581 gccccagggc ttcgatggct tcggaggcga tcaacgtgat ctcgcggccg gagccggggg
     5641 tcttggagta ggtgccggtc tggcggaaat agcggtctcc gtccagcccc ttgccgggga
     5701 cggcgcggac ctccttgacg gagatcatgt tccccgcggc ggcaggggtg atgtggatgg
     5761 agagcacggt gcccttgaac atgcctgagg cctccctctc gcgctgggtc aacgcagcac
     5821 ggatgcctgt tttgccggcc ggtccccaag atccttgtgc cccggagcag gcgggaggag
     5881 accatccgcg atgatccgaa cgagtccaag agagcaggag gagtccggga gatgggagca
     5941 gcgaacaagg tcgccatcgt caccggcgcc ggcacaggca tcggcagggc ggtgaccatg
     6001 gccctggcac aggatggata ttcggtcgtc ctcgccgggc ggcgcaggga gcggttggat
     6061 gccacggccc gggcggtcgc ggcatcggaa acgcgcacgc tggtcgtgcc caccgacgtc
     6121 accgatcccg cgtccgtcag agccctgttc gccaggacga aggaggcctt cggccggctc
     6181 gacctgctgt tcaacaacgc tgggatcggt gcgcctcccg tcccgctcga ggacctcccg
     6241 tacgagcaat ggaaggcggt cgtcgagacc aatctctcgg gagccttcct ctgcacccag
     6301 gaagcgttcc ggctcatgaa gagccagacg ccccgcggcg gccgcatcat caacaacggc
     6361 tccatttccg cgcacaaccc gcggccgaac tccgcaccgt ataccgccac caagcacgcg
     6421 atcaccggcc tcacgaagtc ggcctcgctc gacggccgga agtacgacat cgcctgcggc
     6481 cagatcgaca tcggcaacgc cgcgaccgag atgacggcgc gaatggtgga gggcgtggcg
     6541 caggctgacg ggtccatccg ccccgagccg aggatggatc cgtcgcacgt ggcgcgcgcg
     6601 gtggtctaca tggcgagcct tccgctggat acgaacgtcc agttcatgac cgtgatggcg
     6661 accaagatgc cgttcgtcgg ccgagggtga gcggatatcg gcgtgcggtg ggtcctgctc
     6721 gcgcagtgcc tcttaagccc gactcctcat gacgctgcgc accgggcaca ccgcccgtgc
     6781 acgaccagag aggctcctcg cgggtcgaag ccccgtcctg cgcgccggac cagtggcatc
     6841 agcaccgtgg gatcgacggg caggtccatc acgcgccggc agacatcgca gaaaaggtgc
     6901 ccgtggggag tggtattggg ttcccatcga gtcgccccga cgggcagggg aatcggctcg
     6961 accaaccgga ggtcggcgag ctcgtggagg atcgcataga cggtcttctg cgcaaacccc
     7021 ggcacggtat cccgaagatg cgcatggagt tcttcagcgg tgggatgatc ctcggcgtgt
     7081 tcgagcgcgc agaaaacggc catccgctgg gccgtgatgc ttcggccttg ggctcgaaac
     7141 gccgccactg cctgcctcgc tgagaaccgc cccactcgga attgccaccc cgttaccggt
     7201 gccagagatg cacggacacg cgctgagtct tgtgccgcaa cgcttgcacg cgccccttcg
     7261 cagtgcgccc ttgacggcta ttcttgactc tagcactcta gaactggttc ggcaaccctg
     7321 acaccccggg cctgctcgtc tcaggaggta tggatgtcac gtcggggcat cagcagcaga
     7381 tcggtactca tactcttggc cgtgctgttg gtgatcggcg tcttggtgtg gcaggcggtg
     7441 acggcaggcg ggtctccgga cccgaccgcc aagcacctca gcccggccgc cgcggtcgtc
     7501 gacaccggca tcctggtctt ccgggaaggg ttggaatcgc tcctggtcct ctccgcgatc
     7561 gccgccagtc tctcacgaac gcactcccgg tggggccgcc cggtcgccac cggggccggg
     7621 atcgcgttcc tcacgaccat cgccacgtgg ttcgtggtgg tcgccatcct ctcggacgtg
     7681 aacgccccgg agctcgacct ccaggccgcg acgggcctgc tcgcggtcct ggtgctgctc
     7741 gtgatcatga actggttctt ccaccgggtg tactggaccg gctggatcac gcaccacacc
     7801 cggaccgggc gcgcgctcgt ggagaatccc gggacaccgt ccgccgtggt ggtccggggg
     7861 ctcgtgttgc tcggcttcgc ctcggtttat cgtgaaggct tcgaggtggt gctcttcctg
     7921 cagagcctcc ggctgcaggt cggggcggac gtggtgttcc tcggcagcct gatcggcgta
     7981 ggcctcacgc tgctcgtcgc ggccctcaac ttcctctcgc actaccggct tccgtacagg
     8041 aagatgctgg tgttgacggg catcctgctg ggtggcgtgc tcgtggtgat ggtgggcgag
     8101 accgtgcagg agatgcagca ggcccactgg ctcgccgcca ccaagctcct ccacttcgac
     8161 ctgccgaact ggatgaacgt ctggttctcc gtgtacaaca gctgggagag cctcacggct
     8221 caagtcctca cggtcgtctt tgtgatcggc tcctacctcg tggccgagta cgtgcagatc
     8281 cggcgccccc gccgccaggc cgtggtcctg gcgcaggagg cgttagacac gcgctcataa
     8341 tgctgcgggc tccagtcgct ggaagtgaag ggcgaatgtc ccaagagctc gagaggtcgc
     8401 cgcacgccct ggtcggcgcg cgcgtcatgc gttcgtgggc cgacgttaga ctac
//
LOCUS       DASQGH010000294         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18316, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000294 DASQGH010000000
VERSION     DASQGH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18316"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VEW91_05780"
     CDS             complement(<1..357)
                     /locus_tag="VEW91_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91125.1"
                     /translation="MSGVEVLPGRAATAAIGARRQQMREIRRHILVYAGLAPLLLIAL
                     FPVYWMAITAFKTDADLVNPAVFPFWFHQPSTISHFTYLFQHTYYGDWISNTMLIAAL
                     ISLITLLTAVPGGYALA"
     gene            complement(354..1283)
                     /locus_tag="VEW91_05785"
     CDS             complement(354..1283)
                     /locus_tag="VEW91_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM91126.1"
                     /translation="MANVELVTGTQIHVGRGAFTRRRLVFGYSLLAPAVLYMVLLVGV
                     PFLFSLYLSLSDASVDAPVARFIGLQNFFAIFQDSVSRLALRNTVLITVIAAVFKGVL
                     GTLLAFLLMQEVRGKKLIRSLIVIPWTLPVAISALAWKWMFDPQFSAINWVAVHLHLM
                     RGWPIWLGEPGLALTSVIIVNVWRGFPFTAIVLLAGFSAVPLELIDAGKVDGASYWQR
                     YTAIVIPIIAPILFVGLLFDTVFTLSDLSVVYLLTAGGPMGATEILPTRALEIGIQAG
                     ALGRGAAISLVLFPILLPVVYVLLRILKRREGL"
     gene            complement(1368..>2136)
                     /locus_tag="VEW91_05790"
     CDS             complement(1368..>2136)
                     /locus_tag="VEW91_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91127.1"
                     /translation="LSARVLKAKGHPTGIQISHCNDANHDWRSVFYDFGVKETDPTGK
                     ELLWDSKELREGMKFGKALYDEGMTAEVFSWDDVADNRYLGSGVACWIPDAISAWRSI
                     QGVNPELYNKIYVGPEPTGPKARLNVVDANVYGIWTFAKNKEAALAFLAYWGNHVKES
                     VAASKGYNMPFLRDAYTKPMPVIATDINGAPFLQDWVKTAVVFGYPGPMTPAASEVNS
                     TYVVPDMMGRYVRGGDLEGSIKWGMEQITAIYARRKG"
BASE COUNT          411 a          689 c          697 g          339 t
ORIGIN      
        1 cgccagcgcg tatccccccg gaacggcggt gaggagcgtg atcagcgaga tcagcgcggc
       61 gatcagcatc gtattggaga tccagtcccc gtagtaggta tgctggaaga gatacgtgaa
      121 gtgcgagatg gtcgagggtt ggtggaacca gaacgggaac acggccgggt tcacgagatc
      181 ggcatcggtc ttgaacgcgg tgatcgccat ccagtacacg gggaagagcg cgatcagaag
      241 aagtggcgcg agaccggcat agacgaggat gtggcggcgg atctcgcgca tctgctgccg
      301 ccgcgccccg atggccgcgg tcgccgcccg acccgggagc acctccaccc cgctcacagg
      361 ccctcccgcc tcttcaggat ccggagcaga acgtagacga cggggaggag gatcgggaac
      421 agcaccaggg agatcgccgc gccccgcccc agcgcgcccg cctggatccc gatctcgagc
      481 gcccgggtgg gtaggatctc ggtcgcgccc atcggcccgc cggcggtcaa caggtacacg
      541 acgctgagat cggagagcgt gaagaccgtg tcgaagagca gccccacgaa gaggatgggc
      601 gcgatgatcg gtatcacaat ggcggtgtat cgctgccagt aactggcgcc gtcgaccttg
      661 cccgcgtcga tcagttccaa cgggaccgcg gaaaacccgg ccaagagcac gatcgcggtg
      721 aaggggaacc cccgccagac gttgacgatg atcacggagg tgagcgcgag cccgggctcc
      781 cccagccaga tcggccaccc ccgcatcagg tggaggtgca cggcgaccca gttgatcgcg
      841 ctgaactgcg ggtcgaacat ccacttccac gcaagcgcgc tgatggcgac cggcagggtc
      901 cacgggatga cgatcaagga ccggatgagc ttcttccccc ggacctcctg catcagcaag
      961 aaggccagca gcgttcccag gacgccctta aaaaccgcgg cgatgaccgt gatgaggacc
     1021 gtattccgca gggcgagacg ggagacgctg tcttggaaga tcgcgaaaaa gttttgcagc
     1081 ccgatgaaac gggcgaccgg ggcgtctacc gaggcatcgc tcaagctcag gtacagggag
     1141 aagagaaacg ggacccccac caggaggacc atgtacagca ccgcgggagc gagcagcgaa
     1201 tacccgaaga ccaagcgccg gcgggtgaac gccccccgcc cgacatggat ctgggtaccc
     1261 gttaccagtt cgacgttcgc cacgccggca cgtcccaacg cgggtttatg ctgccgtccc
     1321 ggacgcgttg ctcggggagg aggcgggtcg ccccctcccc ggcgtctcta tcctttgcgc
     1381 ctcgcgtaga tcgcggtgat ctgctccatc ccccacttga tggagccctc gaggtccccg
     1441 ccgcgcacgt accgccccat catatcgggg acgacgtacg tcgagttgac ctctgatgcg
     1501 gccggcgtca tcggcccggg atacccgaac accacggccg ttttcaccca atcctggagg
     1561 aacggggccc cattgatatc cgtcgcgatc acgggcatcg gcttcgtgta ggcatcccgg
     1621 aggaacggca tattgtagcc cttgctcgcc gcgaccgact ctttcacgtg gttcccccag
     1681 tacgcgagga acgccagcgc cgcctccttg ttcttcgcga acgtccagat gccgtagacg
     1741 ttggcgtcga cgacgttgag gcgtgccttg gggccggtag gttcgggtcc gacgtagatc
     1801 ttgttgtaca gctcggggtt gacaccctgg atcgaccgcc aggcggagat cgcgtcgggg
     1861 atccagcacg cgacgcccga gcccaggtat cggttgtccg caacgtcgtc ccaggagaag
     1921 acctccgcgg tcatgccctc atcgtagagg gctttgccga acttcatccc ctcccggagc
     1981 tccttggagt cccagagaag ctcctttccg gtggggtcgg tctccttgac gccgaaatcg
     2041 tagaagaccg accgccagtc gtggttcgcg tcgttgcagt gggagatctg aatgcccgtc
     2101 ggatggcctt tggccttcag cacccgcgcc gagagg
//
LOCUS       DASQGH010000295         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43716, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000295 DASQGH010000000
VERSION     DASQGH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43716"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="argH"
                     /locus_tag="VEW91_05795"
     CDS             complement(<1..789)
                     /gene="argH"
                     /locus_tag="VEW91_05795"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYM91128.1"
                     /translation="MPEARRGSRNDPATARMWGGRFRGELDPQVAAFTTSLPFDKRLY
                     RADILASIAHATMLGRCGIVPAEEADRLVQGLREILREADAGILEIQGAEDIHTFVEA
                     ALTTRLGDAARRLHTARSRNDQVATDLRVYLKAEIVKIIGRTVALQQVLLAMAEAHPA
                     VIIPGYTHLQRAQPVLLAHHLLAYVWMLHRDVGRFRDAFARTDALPLGAAALAGTSYP
                     IDPRLVADLLGFSRVAENSLDATSDRDFAVEFVSAAALLMTHLSR"
     gene            complement(794..>1684)
                     /locus_tag="VEW91_05800"
     CDS             complement(794..>1684)
                     /locus_tag="VEW91_05800"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYM91129.1"
                     /translation="FELAYQALAPQLGVIAPWREWTLGSREEEIEYARTHGVPVPVTR
                     EEPYSMDRNLWHLSFESGVLEDPWAAPPAGMHRLTVDPEKAPDAAAYVEIGFERGTPT
                     SVDGRALDPVALVAHLNRLAGAHGVGRVDLVENRLVGMKSRGVYETPGGTVLAEAHHH
                     LETITLDRETQHYKALVAPKYAELVYNGLWYSPLRRALDAFVATTQETVAGTVRLKLF
                     KGSAVVIGRRAPRSLYSHDLATFGRSTGYDQGDAAGFIRLFGLPTKVFAEVNPDLSRD
                     LRPPRAKVPAPGSGPKPPFG"
BASE COUNT          241 a          586 c          611 g          246 t
ORIGIN      
        1 cctcgacagg tgggtcatca gcagcgccgc cgccgagaca aactcgacgg cgaagtcccg
       61 gtcggacgtc gcatccaggc tgttctccgc gacccgggag aaccccagga gatcggcgac
      121 caggcgcggg tcgatcggat acgacgtgcc ggcgagcgcg gcggcgccga ggggaagcgc
      181 atcggtccgg gcgaatgcgt cgcggaaccg gcccacgtcc cggtggagca tccacacgta
      241 tgcgagcagg tgatgcgcga ggaggacagg ttgcgctcgc tggaggtgcg tgtagccggg
      301 gatgatcacg gcaggatgcg cctcggccat cgcgagcagc acctgctgga gcgccacggt
      361 gcgcccgatg atcttgacga tctcagcttt cagatacacc cgcaggtcgg tcgcgacctg
      421 atcgtttcgc gagcgcgcgg tgtggagccg ccgtgcggcg tcgccgagcc gcgtggtgag
      481 cgcggcctcc acaaacgtgt ggatgtcctc cgccccctgg atctcgagga tccctgcgtc
      541 cgcctcgcgc aggatctcgc ggagcccctg gaccagccgg tcggcttcct cggccgggac
      601 gatcccgcac cggccgagca tcgtggcgtg cgcgatgctg gcgaggatat cggcccggta
      661 caagcgcttg tcgaacggga gcgaggtcgt gaaggcggcg acctgcgggt cgagctcgcc
      721 tcggaagcga ccgccccaca tccgggcggt ggccgggtcg ttcctggatc cgcggcgggc
      781 ctctggcaca gcgttacccg aagggcggct tcggcccgga tccgggcgcg ggcaccttgg
      841 cccgcggggg ccgaaggtcg cgggagaggt ccgggttcac ctcggcgaag accttggtcg
      901 gtaggccaaa gaggcggatg aacccggccg cgtccccctg gtcgtagccg gtactgcgcc
      961 cgaacgtggc caggtcgtgg ctgtacagcg acctcggggc gcggcggccg atcacgaccg
     1021 cgctcccctt gaacagtttg agccgcaccg tgccggccac ggtctcctgc gtggtcgcga
     1081 cgaacgcgtc caacgcgcgg cgcagcggag agtaccacag cccattgtag accagctccg
     1141 catacttcgg cgccaccagc gccttgtagt gctgggtctc gcggtccagc gtgatggtct
     1201 cgaggtggtg gtgcgcctct gccagcacgg tgccgccggg ggtctcgtac acgccgcggc
     1261 tcttcatgcc gaccaggcgg ttctcgacaa ggtcgacgcg ccccaccccg tgcgcgcccg
     1321 caaggcggtt gaggtgggcc accagggcga cggggtccag ggcgcggccg tccacgctcg
     1381 tcggcgtccc ccgctcgaac ccgatctcga cgtaggcggc ggcgtccggc gccttctccg
     1441 gatccacggt cagccggtgc atcccggccg gcggcgcggc ccaggggtcc tcgaggaccc
     1501 cgctctcgaa gctgagatgc cagaggttcc ggtccatact gtagggctcc tcgcgggtca
     1561 ccggcaccgg caccccgtgg gtgcgcgcgt actcgatctc ctcctcgcgc gagccgaggg
     1621 tccactcccg ccacggtgcg atcaccccaa gctggggggc cagggcctgg taggccagct
     1681 cgaa
//
LOCUS       DASQGH010000296         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55026, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000296 DASQGH010000000
VERSION     DASQGH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55026"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..709
                     /locus_tag="VEW91_05805"
     CDS             41..709
                     /locus_tag="VEW91_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM91130.1"
                     /translation="MDDEPHIVELVRYNLAQEGFEVSVAYDGHEAIEKAKQESPDLVI
                     LDLMLPYVDGLEVCRHIRRESSVPILMLTAKDGEQERVVGLELGADDYVTKPFSPREL
                     VARVRAILRRTVREAQRPAAKPVAVGGLVLDPTTHEVRLNGRPIELTAKEFDLLQILL
                     SHPNRVFTRDFLLEHIWGYDYFGGTRTVDMHISRLREKIEDDPNTPTYVMTVRGVGYK
                     LKRD"
     gene            712..2520
                     /locus_tag="VEW91_05810"
     CDS             712..2520
                     /locus_tag="VEW91_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM91131.1"
                     /translation="MNRSLRWKLTGSYLLLVLFSLAVAAGVLIPAITQVYTNDYQRQV
                     LNQAGIITRLLETHQAEGASIARLDEIASRSSWREGVYVGVKDATGQPPPMSRWAGQW
                     GGPVGPEIQAALEGRPATVIRPDPANGEIRIFAATPLKAQGVLIGVVQVSVPRLWLDR
                     ALEKVWMALGEALLAGVVAALLLGAWRTRALAAPVLELARAARRISAGDLTARVAVQG
                     RDEIGQLAGTFNAMAEQLRQKLAAVSEERNKIEAIISSMSDAVVAVDREGTILLLNRA
                     AEDLLGLPQTARGRRLADVLGEHPFRRSIEAAAQDRRDVAEEFSPHPLDDRTLDVHAT
                     PLCGPEGDNAGAVAVVRDVTDLRRAERLRRELTANVSHELRTPLTSIKGFAETLLGGA
                     MADEAACRRFLTIIDGEATRLMKLVDDLMDLSRLESRAVSMEPAEFRVDELVAEALSR
                     MRPQADRHRIALRARTVPAVALADRDRVLQVLTNLLDNAIKFTPEGGTVDVSVTSPPT
                     GAVVSVTDTGRGIPADDLPRIFDRFYRVDRSRSREAGGTGLGLAIAKHIVEAHGGRIT
                     VTSRVNAGSAFTFTLPLASPLRGAPALERPTPLLGD"
     gene            2950..3393
                     /locus_tag="VEW91_05815"
     CDS             2950..3393
                     /locus_tag="VEW91_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYM91132.1"
                     /translation="MAAPWAYMDTSVLLKRYLDESGSAEARRLLRRYRVVSSAIAPVE
                     ATSALWQRRAVGDVAETDLQAILKRLAEDRSHWEFVDLTTDVLDRAEHVIRRTGVRTL
                     DAVHLASVLTIHAANVQRRSAPFITGDARQRDAATGLGLQVTWVG"
     gene            complement(4162..4281)
                     /locus_tag="VEW91_05820"
     CDS             complement(4162..4281)
                     /locus_tag="VEW91_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91133.1"
                     /translation="MELRTALQTALRTDRPALIEVPVGEMPSPWPVIRAGFRG"
BASE COUNT          727 a         1440 c         1450 g          723 t
ORIGIN      
        1 gacgaaggcg gagcagcagg ccgggggccg gattctggtc gtggacgatg agccgcacat
       61 cgtcgagttg gtgcggtaca accttgccca ggaggggttt gaggtttcgg tggcctacga
      121 cggccacgaa gccatcgaga aggcgaagca ggaatccccc gaccttgtca tcctggatct
      181 catgctcccc tacgtggacg gcctggaggt ctgccggcac atccggaggg agtcgtcggt
      241 cccgatcctc atgctgaccg ccaaggatgg ggagcaggag cgggtcgtcg ggctcgaact
      301 gggtgcggac gactatgtga ccaaaccgtt cagcccgcgc gaacttgtgg cccgggtccg
      361 cgcgatcctc cgccgcacgg tccgggaggc ccagcggccg gcagccaagc cggtggccgt
      421 gggcgggctt gtcctcgatc cgaccaccca cgaagtccgc ctcaacggcc ggccgatcga
      481 gctcacggcg aaggagtttg acctcctgca aatcctgttg agccacccga accgggtgtt
      541 cacccgggac ttcctcctcg aacacatctg gggctacgac tacttcggcg gcacccggac
      601 cgtggacatg cacatcagcc ggctccggga gaagatcgag gacgatccca acaccccgac
      661 gtacgtcatg accgtgcgcg gggtgggcta taaactgaag agggattagg cgtgaaccgc
      721 agcctgcgat ggaagctcac cgggagttac ctgctgctcg tcctcttctc cctggcggtc
      781 gccgccgggg tcctgatccc ggccatcacc caagtctaca caaacgacta ccagcggcag
      841 gttctgaacc aggccgggat catcacgcgc ctcctcgaga cccaccaagc agagggagcg
      901 agcatcgccc gcctcgatga aatcgccagc cggtcctcgt ggagagaggg cgtctacgtg
      961 ggcgtcaagg acgcgacggg gcagccgccg ccgatgagcc ggtgggcggg acagtggggt
     1021 ggcccggtcg ggcccgagat ccaggccgcc ctcgagggac ggcccgccac cgtgatccgc
     1081 cccgacccgg ccaacgggga gatccggatc ttcgccgcga ccccgttgaa ggcccaaggc
     1141 gtgctcatcg gggtggtcca ggtgagcgtg ccccggctgt ggctcgatcg ggcgctcgag
     1201 aaggtgtgga tggcgctcgg cgaggcgctg ctggccggcg tcgtcgccgc gctgctgctc
     1261 ggcgcctgga ggacgcgcgc gctcgctgcc ccggtcctgg aactggcccg cgccgccaga
     1321 cggatctccg cgggcgatct caccgcccgc gtcgcggtcc agggccgcga tgagatcggg
     1381 cagctcgccg ggacgttcaa cgcgatggcg gagcagctcc gtcagaagct ggcggccgtc
     1441 agcgaggaac ggaacaagat cgaggccatc atctcgagca tgagcgatgc cgtggtggcg
     1501 gtggatcgcg aggggacgat cctgctgctc aaccgggccg ccgaagacct cctcggcctg
     1561 ccgcagacgg cgcgggggcg gcgtctcgcg gacgtgctcg gcgagcatcc gttccggcgg
     1621 agcatcgagg cggccgccca ggaccggcgg gatgtcgcag aggagttcag cccccacccc
     1681 ctggacgacc ggaccctcga cgtgcacgcc acgccgctct gcgggccgga gggagacaac
     1741 gccggcgcgg tggccgtcgt ccgggatgtc accgatctcc ggcgcgcgga gcgcctccga
     1801 cgggagctca cagcgaatgt ctcccacgag ctccgcacgc cgctcacctc gatcaaagga
     1861 ttcgccgaga cgctgctggg gggggccatg gccgatgagg cggcgtgccg gcggttcctg
     1921 acgatcatcg atggcgaagc gacccgcctg atgaagctgg tcgacgacct gatggacctc
     1981 tcgcgcctgg aatcgcgggc ggtctcgatg gagcccgccg agttccgggt cgacgagttg
     2041 gtcgcggaag ccctgagccg catgcgcccg caggccgatc gccaccggat cgcgctgcgg
     2101 gccaggaccg ttcccgcggt cgcgctggcc gatcgcgatc gggtcctcca ggtcctcacc
     2161 aatctgctcg acaacgcgat caagttcacg cccgaggggg ggacggtgga cgtgtcggtg
     2221 accagccccc ccaccggcgc ggtcgtgtcg gtcaccgata ccggccgggg gatccccgcc
     2281 gacgacctcc ccaggatctt tgaccggttc taccgcgtgg atcgctcgcg atcgcgcgag
     2341 gccggcggca cgggcctggg cctcgccatc gccaagcaca tcgtcgaggc gcacggcggc
     2401 cggatcaccg tgacgagtcg cgtcaatgcg ggaagcgcgt tcacgtttac gctcccgctc
     2461 gccagcccgc tgcggggcgc cccagccctg gagcgcccga cgccgcttct cggcgactga
     2521 tcctaccaag ttcacggaga ggacctgtcg aggcaacaca ggttctctct gtttatttct
     2581 cccaatgcta gcgacattcc ggttgacaga gggaccgggt cggcgtagga tggcagtagt
     2641 gtagtgacgt tactactcac aaggtgcgcc gatgaagatg ggcttgcggg aagcgaatca
     2701 gcagttttcc cgagcgatca gggcagtgcg ggccggacgc gaagtagtcc tgacggagcg
     2761 tggcacgccc atcgcggtga tccggccgat cccgcgctcc gtcagtcccg atgaggtgct
     2821 gcaccgcctg gaggaggctg gaatcctacg gcgggctgcg aagcggacgc caatgccatc
     2881 atggaagccg cggccgattc gcggagtttc tatcgttcag acgctgcgcg aggagcggga
     2941 ggtctcctga tggctgcccc ctgggcgtat atggacacga gtgtcctcct gaagcgctat
     3001 ctcgatgagt ctggatcggc ggaagcgcgc cgactccttc ggcgataccg tgtggtatca
     3061 tcggcgattg cgccggtaga ggcgacttcg gcgctctggc agcgacgggc cgtaggggat
     3121 gtggcagaaa ctgatcttca ggcgatcctg aagcgcctgg cggaggaccg gtctcattgg
     3181 gagtttgtcg acctcacgac cgacgtactc gatcgggccg aacacgtcat tcggcgtacc
     3241 ggagtccgaa cgttggatgc cgtccacctc gcatccgtcc tgacgatcca tgcagcgaat
     3301 gtgcaacgcc gatcagcacc gttcattacg ggtgacgccc ggcaacgcga cgcggcgacc
     3361 gggctggggc ttcaggttac ctgggttggc tgacggctag ctcctgggac ccaaatccct
     3421 agccgacttc aatcttgcag agcacggggt gctctgttcg aaggttgcgc gtccccgcta
     3481 ccactggtat cgacatcggg ggatgtctcc tcggagttca gcaacgagac ccgctggagg
     3541 agccggccgg gacacgggta cgcctgggat gcagtcccgg tcccggccag ccctcggtgc
     3601 caccctcatt ggatccgccg cgtcaaccta cttgggtttc gcgtagtcgg tcgcgcctgt
     3661 gatatcgatc atcacgaccg gctcgttccc aacaacccag gcatcatgtc caggaggaac
     3721 cacccccaca tcgcctgggc caaactcttg ctctgtgcca tcgtccatcc tcacccgcag
     3781 ccgccccgaa acatgatagg acgtgtgcgg agcttggcag ctcttggtct tggctaacgg
     3841 tttcaccgat tcggaccact tccatcctgg ctcaaaagtg gcccggccaa acgtgatgtt
     3901 cccgagtttc gcaagctcga gcctcccttt ggggaaggac cgcttttcgt ctggcgaatt
     3961 caagttcttt ttcgatattg ccgccatgtt tcccacgctc ctttgagtcg cgcctgggtg
     4021 tactgatact ccttctcgcc gtccgtctct ggatcctccc caaaatgtcc ggcggcttgg
     4081 atcagcggca cacggggagc gagcggtcac cgcgatcggg ctcggacgac ctgtcaccta
     4141 tagcaaggga ggacgtaccg gctatccccg gaaccctgcc cgaatcaccg gccaaggact
     4201 gggcatctcc ccgacgggca cctcgatcag cgccggccgg tcggtgcgga gcgccgtctg
     4261 gagcgccgtg cgcagctcca ctgggttgga cgccctgagc ccctggatcc cgaacgcttc
     4321 ggccaatctg acgaaatccg
//
LOCUS       DASQGH010000297         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_23963, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000297 DASQGH010000000
VERSION     DASQGH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23963"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2937)
                     /locus_tag="VEW91_05825"
     CDS             complement(<1..2937)
                     /locus_tag="VEW91_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYM91134.1"
                     /translation="MLSQFFIHRPNFAMAISVLIVLVGALSYVSLPREQYPNISPPTV
                     TVSTTYIGASAQVVAQNVAVPIEETVNGVPNMLYMQSRSTSAGQYSLTATFALGTDPD
                     IDAVQVQNRVSEAAGNLPAAVNNFGITVRKASPNFLMGVALYSPQDSYDPTFLTNYAL
                     IHIVDSLLRLPGIGDNLVFPQRSYAIRAWLRPDALAALQLTAGDVVNAITAQNLVAPA
                     GTLGQPPANPNTQFQYTVNAQGQLTDPTQFNRVVVKTLPDGSVVRLQDVATTELGAQD
                     YGTYGLRNGHPAAVIVLLQAPGSNALQASRAVRATMAGLARSFPPGLTYDVVFDTTLF
                     INAALTDVTRTLGIAFLLVLLVVFVFLGKVRATLIPMLAIPVSLVGTFAVFNALGFTI
                     NILTMFALVLAIGLVVDDAIVVVEAVERHIEDGLDPVAAAEGAMRLVQGPVIAIALVL
                     DAVFIPTAFIGGISGRLYRQFALTLAGSVTLSALVALTLTPALSALLLRPRTASRGSA
                     GGLLGGLHAAFIRATDAYIRGVQRCIRGRWLMLGALAVIAGLTFGLLRVIPSTFVPLE
                     DQGFFVSAFRLPDGASLERAQAVAVKATRFLLHLPGVEGVTTIGGYDILTGSFNSNTL
                     AMFVVFRPWEERRSRDAQLFPILQRVNREFAGYPEAIGFAFPLPPLPGVGAVNGFQFM
                     VEDRNGTGQADALARVVQAVMGAAGTHREVTALSTGFSASVPQFNVAVNRDKAGTLGV
                     PVDSIFQNLSAFLGGLQINNVTLFGRVYKAMIQDAPQYRMTPASIGEIYVRSANGGMV
                     PLSTLVTVSPTTGPVLIPRYNGYYAAEVDGQAPFGASSARAINAMEQIAQTLPAGYGY
                     EWTGLAYQEKQAAGATSLIFALALVLVFLLLVALYESWSIPLSVLLGVPLAAFGSLLA
                     VFLRGMVLRDVLSDVYVQIGLVTLVGLAAKNAILIVEYAKNKHEQEGMPVVEAAIAGA
                     QLRLRPI"
     gene            complement(2952..4280)
                     /locus_tag="VEW91_05830"
     CDS             complement(2952..4280)
                     /locus_tag="VEW91_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYM91135.1"
                     /translation="MRWVTSLALGVLGFALVACASSRGGTQAPRGAPPPPPPGVVVTA
                     AIERTVPIYTDAVAQVVAAQTVDLRAQVAGTLEEVLFKQGTEVKRGQLLFVIDQRPYM
                     AALQSAQAQLAAAQAALKQALEQVQLRQAEAQLVALKATLVNAQQQVARDRYLVAQQA
                     LAQQQLDNDTAQEQAAEANVAAQEAVVKNTALSQQIAIEQARAGVQQAQAAVTQAQLN
                     LVYSTVRAPIDGVIGLLNVDQGNLVSINTQLATMSSVDPIVAQFPLSEVTFLGLVKRT
                     TAEAARAGLTPLNFPSFQLILADNSTYRHAGRFRTLNRAVDPQTGTIVVQALFPNPER
                     LLRPGMFARVRAKIEDRPHTVLIPQAAVQEVQGARTVFIVEPDATVVLRSITEGGPYG
                     AFFIVLGGVHAGERVIVEGLQKVRPGMQVTPTPRPAPPLPPEGPATGQNG"
BASE COUNT          768 a         1424 c         1573 g          697 t
ORIGIN      
        1 aatcgggcgc aggcggagct gcgccccggc aatggccgcc tccacgaccg gcatcccttc
       61 ctgctcatgc ttgttcttgg catactccac gatcaggatc gcgttcttcg ctgccaggcc
      121 aacaagggtc accaggccga tctgcacgta cacgtcgctg aggacatctc ggagcaccat
      181 gcctcggaga aagacggcca ggagcgagcc gaacgcagcg agcggcaccc cgaggaggac
      241 gctgagggga atgctccagc tctcatacag ggcgaccagg agcagaaaca ccagcacgag
      301 ggcgagcgca aagatcaggg acgtcgcccc cgcggcctgc ttctcctggt acgccaatcc
      361 cgtccattca tagccgtacc cggccgggag cgtctgggcg atctgttcca tcgcgttgat
      421 ggcccgagcc gaactggcgc cgaacggggc ctgcccgtcc acttcggccg cgtagtatcc
      481 attgtagcgc ggaatgagca ccgggcctgt ggtcggactg accgtgacga gcgtgctcaa
      541 gggcaccatc ccgccgttcg cactgcggac gtagatctcg ccgatgctgg cgggggtcat
      601 ccggtattgc ggcgcgtcct ggatcatcgc cttgtacacg cggccaaaaa gggtcacgtt
      661 gttgatctgc aggcctccca gaaacgccga cagattttgg aagatgctgt cgacgggcac
      721 ccccaacgtc ccggccttgt cccggttcac cgcaacattg aactgaggga ccgacgcgct
      781 gaacccggtc gagagggcgg tgacctcccg gtgcgtgccg gcggcgccca tgaccgcctg
      841 cacaacccga gcaagcgcgt ccgcttgtcc cgtgccgttc cggtcctcca ccatgaactg
      901 gaagccgttg acggctccca ctcccgggag gggcgggagg gggaatgcga acccgatcgc
      961 ttccgggtag ccggcgaatt cgcgattgac gcgctgcagg atcgggaaga gttgcgcgtc
     1021 ccgggaccgc cgctcttccc acggccgaaa cacgacgaac atggcgagcg tgttggaatt
     1081 gaacgatccg gtgaggatgt catatccgcc aatcgtcgtc acaccttcga cgccggggag
     1141 gtggagtaga aaccgcgtgg ctttaacggc gaccgcctgc gctcgctcca gcgacgcccc
     1201 gtcgggcagc ctgaaggctg agacgaagaa gccctggtcc tcgaggggca caaaggtcga
     1261 ggggatgacc ctcaggagtc caaaggtcag ccccgcgatc accgcgaggg cgccgagcat
     1321 gagccagcgc ccccggatgc agcgttgcac cccccggatg taggcgtcgg tggcccggat
     1381 gaaggcggcg tggagtcccc cgaggagccc ccccgcggat ccccgcgagg cggtgcgcgg
     1441 gcgaaggagc agcgcggaca acgccggcgt gagggtgagc gcgacgaggg cggagagcgt
     1501 aacagacccg gccagggtca gggcgaactg ccggtagagg cgtccgctga tgcccccgat
     1561 gaaggctgtg gggatgaaga cggcgtccag gacgagtgcg atcgcgatga cgggcccctg
     1621 caccaggcgc atcgcccctt ctgcggcggc cacggggtcg aggccatctt cgatgtggcg
     1681 ttcgaccgcc tcgacgacga cgatcgcatc gtccacgaca aggccgatcg ccaggaccaa
     1741 cgcgaacatg gtcaggatgt tgatggtgaa gccgagcgca ttgaagacgg cgaaggtacc
     1801 cacgagcgag acgggaatcg ccagcatcgg gatgagggtg gcccggacct tgcccaggaa
     1861 gacgaacacg accaggagga ccaacagaaa cgcgatcccc agggtcctcg tcacatcggt
     1921 gagcgcggcg ttaatgaaca gcgtggtgtc aaagacgaca tcgtaggtga gaccgggcgg
     1981 gaatgaccgg gccagacctg ccatggtcgc ccggaccgcc cgggacgcct ggagcgcatt
     2041 cgacccgggg gcctggagca agacgatcac ggcggcgggg tggccgttgc gaaggccata
     2101 cgtgccgtag tcctgcgcgc cgagctcggt cgttgcgacg tcctgcagcc ggacgacgct
     2161 cccatctgga agggttttca cgacgacccg attgaactgg gttgggtcgg tgagctggcc
     2221 ctgggcattg accgtgtact gaaactgcgt gttcgggttg gcggggggct gccccagggt
     2281 cccggccgga gccaccaggt tctgcgccgt gatggcattg acgacgtcgc ccgcggtcaa
     2341 ctgaagggcc gccagcgcat ccggccggag ccaggcccgg atcgcatagc tccgctgcgg
     2401 gaagaccagg ttgtcgccga tcccgggaag acgcagcagg gaatcgacga tgtggatgag
     2461 ggcgtagttg gtgaggaagg tggggtcgta gctgtcctgg ggggagtaga gcgcgacgcc
     2521 cattaagaag ttgggggacg cctttcgcac cgtgatcccg aagttattta cagcggccgg
     2581 caggttgccc gcggcctcgc tcacccggtt ttggacttga accgcgtcga tgtcggggtc
     2641 ggtgcccagc gcgaacgtgg ccgtgagcga gtattgcccc gcggacgtgc tccgggactg
     2701 catgtagagc atgttgggga cgccgttgac ggtctcctca atcggcacgg cgacgttctg
     2761 cgcgacgacc tgggcgctcg ccccgatgta ggtcgtcgag accgtgactg tcggcgggct
     2821 gatattcggg tactgctccc gcggcagcga gacgtaggag agcgcgccga ccaggacgat
     2881 caggaccgag atcgccatcg cgaagttcgg ccggtggatg aagaactggc tcagcaccgc
     2941 cggtgtcccg cctacccgtt ctggcccgtg gcgggcccct cgggggggag cgggggcgcg
     3001 ggccgtggcg tcggggtcac ctgcatgccg gggcgcacct tctgcaggcc ttccacaatc
     3061 acgcgctcgc cggcgtgcac cccgccgagg acgatgaaga aggcgccgta cggtccgccc
     3121 tcggtgatcg atcgcagcac gacggtggcg tccggttcga cgatgaacac ggtccgggcc
     3181 ccctgcacct cctgcaccgc ggcctggggg atgagcacgg tgtgtggccg atcttcaatc
     3241 ttcgcgcgca cccgcgcgaa catccccggc cgcaggagcc gttccggatt cgggaacagc
     3301 gcctgtacga cgatggtgcc tgtctggggg tcgacggccc ggttgagcgt ccggaatctg
     3361 cccgcatggc ggtaggtgct gttgtcggcc aggatcaact ggaacgacgg aaaattcaga
     3421 ggagtcaggc ccgccctcgc ggcctcggcg gtggtccgct tgacgaggcc gaggaacgtg
     3481 acttcgctga gcgggaactg cgcgacgatg gggtccaccg aggacatggt cgcgagctgc
     3541 gtgttgatcg agaccaggtt gccctggtcg acgttcagca gaccgatgac gccatcgatc
     3601 ggcgcgcgaa cggtcgagta gaccaggttc agttgcgcct gggtgacggc ggcttgggcc
     3661 tgctggacgc cggcccgtgc ctgctcaatc gcgatctgct gcgacagggc ggtattcttg
     3721 accacggcct cctgggccgc cacattggcc tccgccgcct gctcctgcgc cgtatcgtta
     3781 tcgagctgct gttgcgccag ggcctgctgg gccaccaggt agcggtcccg ggcgacctgc
     3841 tgctgggcgt tcaccagcgt cgccttgagg gccaccaact gcgcttcggc ctgacgcagc
     3901 tggacctgct cgagcgcttg cttgagcgcc gcctgcgcgg cggcgagctg ggcctgggcg
     3961 gactggagcg ccgccatgta cggccgctgg tcgatgacga agagcagctg gccgcgcttg
     4021 acttccgtgc cctgcttgaa gagcacctcc tcgagggtcc ctgcgacctg ggcgcggagg
     4081 tcgacggttt gagccgcgac gacttgcgcg acggcgtcag tataaatcgg gacggtgcgc
     4141 tcgatcgctg ccgtgaccac tacacccggg ggcggcggcg gcggcgcgcc cctgggggct
     4201 tgtgtccccc cgcgggatga ggcgcaggcc acgagggcga acccgagcac gcccaaagcg
     4261 agcgaggtga cccaccgcaa cacacatctc cgggtgacca tcaggctatc agatcatacg
     4321 gaccacgcgg gggccaaaat tccctgcacg cgtgggtgcc aggaggcgcg tgctttgtga
     4381 gcgcggcgcc gatcacgccc ctcgcacttc gacccggatc ccgatggggt cggtgagcac
     4441 tagcgccgag tctacgtgct cg
//
LOCUS       DASQGH010000298         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52155, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000298 DASQGH010000000
VERSION     DASQGH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52155"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..911
                     /locus_tag="VEW91_05835"
     CDS             120..911
                     /locus_tag="VEW91_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM91136.1"
                     /translation="MTGTEPWALILGASSGFGEATSLELARNGYDIIGVHLDRKSTLP
                     HVERVIDEIRATGRRALFFNVNAADPERREEVLFRASDEMGRSGTVAVLLHSLAFGNL
                     RPYIAGTPQDEITAAQMDMTLTVMAHSLVYWTQDLVRRGLIRQGGKVFAMTSAGGTRV
                     LPNYGAVSAAKAALESHIRQLAMELAPRGIAVNAIRAGVTDTPALRKIPGHEAIAETA
                     LKRNPSGRLTIPGDVARAIAALCRPGTEWITGNVIGVDGGEDVIG"
     gene            complement(922..2067)
                     /locus_tag="VEW91_05840"
     CDS             complement(922..2067)
                     /locus_tag="VEW91_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HYM91137.1"
                     /translation="MQQAPGSPAPISEGLEGIVVSDSRICFVDGIQGRMLYQGYDIHD
                     LAQHATFEEVAYLLWHGELPTRPQLAQLKSDLAGSRPLPEPVMRAVQAFPPDALPMDI
                     LRTAVSTLGIYDPDSRDNTEAANVRKSMRLTAQVATIVAAIGRLRAGKPPVAPDPGLG
                     HAANFLYMLGGKRADEESTRAIDVALVLHADHELNASTFSARVTAATLADMHSAITSA
                     IGTLKGPLHGGANENVMRMLLGIGDPAKAVPAVAGMLAARQKIPGFGHRVYKTEDPRA
                     THLRRMSETLGRQRGHPEWFEMARAIEQHMLQEKHIYANVDFYSGSVYYVLGIPIELF
                     TPVFAVSRISGWTAHVLEQYADNRLIRPRAEYVGPRDRTFVPIEQRG"
     gene            complement(2104..2916)
                     /gene="trpA"
                     /locus_tag="VEW91_05845"
     CDS             complement(2104..2916)
                     /gene="trpA"
                     /locus_tag="VEW91_05845"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYM91138.1"
                     /translation="MTPGPGNRLAGALAELRAHNRRALIPYIMAGDPDPQATERLVEI
                     LGAAGADAVELGVPFSDPIADGPVNQRAGLRALAHGTSLRRTLDLVARLRERTGIPLA
                     IMTYYNLIFRYGLDAFCRDAVGAGLDGLIAADLPPDEAADLIAAARPVDLATVFLLAP
                     TSTEARIRAVAAASTGFVYCVSRTGVTGVRDDLPEGVRDLVLRIREHTATPICVGFGL
                     SRPEQVREVTAVADGVIVGSAVVKMIEEAPLALDRVGAFVRGLRAAIDDRPK"
     gene            complement(2913..4118)
                     /gene="trpB"
                     /locus_tag="VEW91_05850"
     CDS             complement(2913..4118)
                     /gene="trpB"
                     /locus_tag="VEW91_05850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYM91139.1"
                     /translation="MSAQPTTVPRSRGRFGAYGGRYVPETLIPVLDELEAEYAAAVRD
                     PAFAAEVDRYLTTFCGRPTPLYFARRLTETLGGARIYLKREDLLHTGAHKMNNTIGQV
                     LLATRMGKRRIIAETGAGQHGVATATAAAVFGLPCDVYMGTEDMERQALNVFRMRLLG
                     ARVVPVETGTRTLKDAINEALRDWVTNVATTLYVIGSVVGPHPYPTLVRDFQSVIGRE
                     ARKQVLAVEGRLPDCAVACVGGGSNAMGLFHPFKDDAAVALVGVEAGGLGLATGQHAA
                     TLCAGSPGVLHGAMSYLLQSPEGQVLPTHSISAGLDYPGVGPEHAYFKDTGRARYVSV
                     TDAEALEGFHLLCRTEGIIPALEPAHAVAYLRTLAPHLGRDKVIVLGLSGRGDKDVHT
                     VAKAMGERL"
     gene            complement(4115..>4472)
                     /locus_tag="VEW91_05855"
     CDS             complement(4115..>4472)
                     /locus_tag="VEW91_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HYM91140.1"
                     /translation="RQFDRPVLKSIKVRGPESIDGLARYRVAAYLLDTLMPGEMGGTG
                     KAFDWSLAVRAKAAGPVILSGGLTPENVGEAIRAVAPYAVDVASGVETDGEKDPAKIR
                     AFIARVHEWNAAEVRR"
BASE COUNT          636 a         1579 c         1596 g          661 t
ORIGIN      
        1 ccggctacgg ggtcctgccg agcttcccat ggcctccccc ctcgggggtc tcttcggttt
       61 agggccaggg gctcctgccc gaggggaccg gcggccctga ggcgaactct gggagcggca
      121 tgacgggcac ggaaccgtgg gcgctgatcc tgggggcgag cagcgggttt ggagaagcga
      181 cgagcctcga gctcgcccgc aatggctacg acatcatcgg ggtccacctc gaccgcaagt
      241 ccacgcttcc ccacgtcgag cgggtgatcg acgaaatccg ggcgaccggc cggcgggcgc
      301 tgttcttcaa cgtcaatgcg gccgacccgg agcgacggga agaggtcctc ttccgcgcct
      361 ccgacgagat ggggcgatcg ggcacggtgg cggtgctcct ccactccctc gccttcggga
      421 accttcggcc gtatatcgcc ggcacccccc aagacgagat cacggcggcg cagatggaca
      481 tgaccctgac cgtgatggcc cacagcctgg tgtactggac ccaggatctc gttcgccgcg
      541 ggctgatccg ccaggggggg aaggtcttcg cgatgacgag cgccggaggc acccgggtgc
      601 tgccgaacta cggggcggtg tcggcggcga aggcggcgtt ggagtcccac atccggcaac
      661 tggcgatgga gctggcgccg cgcggcatcg cggtcaacgc catccgggcc ggggtgaccg
      721 atacccccgc cctccgaaag atccccgggc acgaggcgat cgccgagacc gccctcaagc
      781 gaaacccctc gggccggctg acgatcccgg gggatgtggc gcgcgcgatc gccgcgctct
      841 gccggcccgg gacggagtgg atcaccggca acgtgatcgg cgttgacggc ggcgaggacg
      901 tcatcgggta gcctcggccc gctagccccg ctgctcgatg ggcacgaacg ttcggtcgcg
      961 cgggcccacg tactccgccc gggggcggat cagccggttg tcggcgtact gctcaaggac
     1021 atgcgcggtc catccgctga tccggctcac cgcgaacacg ggcgtgaaca actcgatcgg
     1081 aatccccagc acgtagtaca ccgaccctga atagaagtcg acattcgcgt agatgtgctt
     1141 ttcctgcagc atgtgctgct cgatcgcgcg ggccatctcg aaccactccg ggtggccgcg
     1201 ctggcggccg agggtctcgg acatccgcct caggtgcgtc gcccgagggt cctcggtctt
     1261 gtacacgcgg tgcccgaagc cggggatctt ctgcctggcc gcgagcatcc cggccaccgc
     1321 ggggaccgcc ttcgccggat cgccgatccc gagcagcatt cgcatcacgt tttcgttggc
     1381 cccaccgtgc agcgggccct tgagcgtccc gatcgcggac gtgatcgcgg agtgcatgtc
     1441 cgcgagcgtc gcggcggtga cccgggcgga gaaggtgctg gcgttgagct cgtggtcggc
     1501 gtgcagcacc agcgcgacat cgatggcacg cgtgctctcc tcgtcggccc gcttgccccc
     1561 cagcatgtac aggaagttcg cggcgtggcc caggccgggg tcgggcgcca caggcggctt
     1621 tcccgcccgc agccggccga tcgcggcgac gatcgtggcg acctgcgcag tgaggcgcat
     1681 cgacttccgc acgtttgccg cctcggtgtt gtcgcgagag tccgggtcgt agatccccag
     1741 ggtcgagacc gcggtccgga ggatgtccat cggcagggcg tccggcggga aggcctgcac
     1801 ggcccgcatg accggctcgg gcagcggccg ggacccggcg aggtcgcttt tcaactgcgc
     1861 gagctggggg cgcgtcggga gctcgccgtg ccagaggagg taggcgacct cctcaaaggt
     1921 ggcgtgctgg gcgaggtcgt ggatgtcgta gccctggtac agcatccgtc cctggatccc
     1981 atccacgaag cagatgcggc tgtccgagac gacgatgccc tcgagacctt cgctgattgg
     2041 cgcggggctg ccgggtgcct gctgcatcga gtgaagacct cctcgcgcgc tcaccgcgcg
     2101 tgatcacttc gggcggtcgt cgatcgcggc tctgaggccg cgcacgaacg ccccgacacg
     2161 gtcgagggcc agcggggcct cctcgatcat tttcacgacg gcgctgccga cgatcacccc
     2221 gtcggcgacc gccgtcacct cgcgcacctg ctcgggccgg ctgagcccaa accccacgca
     2281 gatgggggtg gcggtgtgct cccggatccg caggaccagg tcgcgcaccc cctcggggag
     2341 gtcgtcgcgc accccggtga cgccggtgcg ggagacgcag tagacgaacc cggtggacgc
     2401 cgcggcaacg gcgcggatcc gcgcctccgt gctggtgggc gcgagcagga acaccgtggc
     2461 caggtccacc gggcgcgcgg cggcgatgag gtcggccgcc tcgtcgggcg gcaggtccgc
     2521 cgcgatgagc ccgtccagcc cggcgcccac ggcgtcgcgg caaaacgcgt cgagcccgta
     2581 cctgaagatc aggttgtagt aggtcatgat cgcgagcggg atcccggtcc gctcgcgcag
     2641 ccgggcgacc aggtccaggg tccggcgcag gctagtgccg tgggccagcg cccgcagccc
     2701 ggcgcgctgg ttgacgggac cgtccgcgat cggatcgctg aatggcacgc ccagctccac
     2761 cgcatcggcg cccgccgccc cgaggatctc caccagccgt tcggtggcct gcgggtccgg
     2821 atccccggcc atgatgtagg ggatcaatgc gcgccggttg tgcgcccgta actcggccag
     2881 cgccccggca agccggttgc cgggcccggg ggtcacagcc gctcgcccat cgctttggcg
     2941 accgtgtgga cgtccttgtc gccgcgcccg ctgaggccga ggacgatgac cttgtcgcgt
     3001 cccaggtgcg gggcgagggt ccgcaggtag gccaccgcat gggccggctc gagcgcgggg
     3061 atgatcccct cggtgcggca cagcaggtgg aacccctcga gcgcctcggc gtcggtcacc
     3121 gagacatacc gggcgcgccc ggtgtccttg aagtacgcgt gctcggggcc gaccccggga
     3181 tagtccagcc cggcggagat ggagtgggtg gggagcacct gcccctcggg gctctgcagg
     3241 aggtagctca tcgcgccgtg gagcaccccg gggcttcccg cgcacagggt ggcggcgtgc
     3301 tgcccggtcg cgagccccaa ccccccggct tcgaccccga caagcgccac cgccgcatca
     3361 tctttaaacg gatggaacag ccccatcgcg ttgctcccgc ccccgacgca ggccacagca
     3421 cagtccggca gccggccctc gaccgccagc acctgcttgc gcgcttcgcg gccgatcacc
     3481 gactggaagt cccgcacgag cgtggggtac ggatgggggc cgacgaccga gccgatgacg
     3541 tacagggtgg tggcgacgtt ggtcacccag tcccgcagcg cttcgttgat ggcgtccttg
     3601 agcgtgcggg tgcccgtctc gaccgggacg acgcgggcgc ccaggagccg catgcgaaac
     3661 acgttgaggg cctgccgctc catgtcctcg gtccccatgt agacgtcgca cgggaggccg
     3721 aacaccgcgg cggcggtggc cgtggcgacg ccgtgctggc ccgccccggt ctccgcgatg
     3781 atccgccgct tgcccatgcg ggtggccagg agcacctggc cgatcgtgtt gttcatcttg
     3841 tgggcgccgg tgtggaggag atcctcgcgc ttcaggtaga tgcgcgcgcc gcccagggtc
     3901 tcggtcaatc ggcgggcgaa gtagaggggg gtcggccgcc cgcagaaggt ggtgagatac
     3961 cggtcgacct cggcggcgaa ggcggggtcg cggaccgccg cggcgtactc cgcctcgagc
     4021 tcgtcgagga ccgggatcaa cgtctccggg acgtagcggc ccccgtacgc cccaaatcgt
     4081 ccccgggacc gcgggacggt cgtcggctgg gcgctcatcg tcgcacctcc gcggcgttcc
     4141 actcgtgcac ccgggcaatg aacgcccgga tcttcgccgg atccttctcc ccatcggtct
     4201 ccaccccgct ggcgacgtcg acggcgtagg gggccacggc ccggatcgcc tcccccacgt
     4261 tctcgggcgt cagtcccccg gagaggatca cgggcccggc cgctttggcc cggacggcca
     4321 aggaccagtc gaacgccttt ccggtacccc ccatctcccc cggcatgagg gtatccagca
     4381 ggtacgcggc cacacggtag cgggccagcc cgtcgatgct ctcggggcct cgcaccttga
     4441 tgctcttgag caccggccgg tcgaactgcc gg
//
LOCUS       DASQGH010000299         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35288, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000299 DASQGH010000000
VERSION     DASQGH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35288"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VEW91_05860"
     CDS             complement(<1..692)
                     /locus_tag="VEW91_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91141.1"
                     /translation="MTAEAALAVEQRRRAGSIWARRLQVFWSSWATRVSAVVVGLLIL
                     VSLVPTGWWPAAASTIDMAVPPHPPSLLAAPFLGTDHLGRDIFYRILRSARLTFIISG
                     LATTFATVTGVLAGLLAGYFRGWTDQAISRLVDFLLAFPVLLLILALVVALGQTATSV
                     IIVLGLSGWAGYTRVMRSAVLALAGLELVDSARAIGASPGRIVFRHLLPNTVSAIMVM
                     STFSLAQFILVES"
     gene            complement(689..1606)
                     /locus_tag="VEW91_05865"
     CDS             complement(689..1606)
                     /locus_tag="VEW91_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91142.1"
                     /translation="MGPYLVGRGFWAVMTIFGIVLVTFLIVRVTGDPSRFVLGVEATP
                     DVVAEFRAEHGLDRPVWEQFWRYLAASAHGNFGSSLRYKEPVLQLFLERLPATLELGA
                     SAYGVAVVVGVVGGVYAAVSAGSPADRTAQVLVLIGQAIPGFYLGLLLILLVSVGLGW
                     FPTGGRGGFSHLVLPTFTLSAYLTALIFRFARSSMLDVLHQDYIRTARAKGIRMVTVI
                     WKHAVRNAMIPLLTVLALQSSVLFSGAVVTETVFSWPGVGRLVVDALLTRDYPLVQAT
                     VAVVTTFVVILNLLVDLAYAYLDPRIRYA"
     gene            complement(1677..1763)
                     /locus_tag="VEW91_05870"
     tRNA            complement(1677..1763)
                     /locus_tag="VEW91_05870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1727..1729),aa:Leu,seq:caa)
     gene            1864..>3477
                     /locus_tag="VEW91_05875"
     CDS             1864..>3477
                     /locus_tag="VEW91_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYM91143.1"
                     /translation="MSSDRPLLILIDANGLVYRAFFALPYFTTSNGQPTNAVYGFATM
                     LLKVLEEESPDYVAVAFDRPGPTFRHEAYAEYKATRRQMPDDLRPQIGLAKEVVEALR
                     LPVFEVAGFEADDIIGALARKAEARGMDVLIVTGDLDTLQLVSPHTRVMMTARGITET
                     TVYDEAAVRQRFGIAPAQIPDLKSLKGDTTDNIPGVPGVGEKTASRLLAQYPSVEALL
                     DAVGEIREPKLRQQLKARREQVLQSKHLATIVTDLDVPLDPEFLRRRPPDQDQVKALF
                     TALEFKTLLERLGVAVPTPQGRGAYRTVRPEALPALLEGAQHLAVALAADPGHPLTAR
                     LHGIAVSARTGEGAYLAGAAIPAPLAGLLEREDLPKYSQDIKHDRLLLEQAGLHPRGF
                     AFDVSLASYLLDPGKRTHTLDAAAWQFLGWRLGAGQAEEGGLALGQAPDEATAEQADA
                     IGRLVEVLDARLRERDLLGLYREIELPLVDVLARMETVGVAIDVEALRALSGTLRARL
                     EALTTEIYRLAGMEFNIGSPKQLAFVLFEK"
BASE COUNT          644 a         1221 c         1130 g          482 t
ORIGIN      
        1 gactccacca aaatgaactg cgccaggcta aacgtcgaca tcaccatgat ggcggacacg
       61 gtgttgggca gcagatgccg gaacacgatc cgacccgggc tcgccccgat ggcgcgcgcg
      121 gaatcgacca gctccagccc ggcgagcgcg agcaccgcac tgcgcatcac ccgcgtgtat
      181 ccggcccacc ccgagaggcc cagcacgatg atgacgctcg tcgccgtctg gccgagcgcg
      241 accaccaacg ccaggatcag gagcaggaca gggaacgcca gcagaaagtc gaccagccgc
      301 gagatggcct gatccgtcca cccccgaaag tatcccgcca gcagcccggc gaggactccg
      361 gtgacggtgg caaacgtcgt cgccagtcca gagattatga atgtaaggcg cgcgctgcgc
      421 aagatgcggt agaagatgtc ccgtcccaga tggtccgtgc ccaggaacgg tgctgcgagc
      481 aacgacggcg gatggggcgg gaccgccata tcgatcgtcg atgcggccgc aggccaccag
      541 ccggtcggca ctaggctcac caagatgagc agcccgacaa ccacggcact gacgcgagtg
      601 gcccatgagc tccagaacac ctggagccgg cgcgcccaga tcgatccggc acgtctcctc
      661 tgctcaaccg ccagtgctgc ctcggctgtc acgcgtatcg gatcctcggg tcgagatagg
      721 catacgcaag gtccaccagc aggttcagaa tgaccacgaa cgtcgtgacg accgcgacgg
      781 tcgcctgcac caggggataa tcacgggtca aaagcgcatc gacgaccagc cgacccacgc
      841 ctggccagga aaagaccgtt tcggtcacca cggcacccga gaagaggacg ctggactgca
      901 gggcgaggac ggtcaacagc gggatcatcg cgttccgcac cgcgtgcttc cagatcactg
      961 ttaccatgcg aatccctttg gccctggcgg ttcgaatata gtcttgatgt aggacatcca
     1021 gcatcgatga gcgggcaaat cgaaagatca acgccgtgag atatgcgctc agcgtgaagg
     1081 tcggcaacac caggtgtgag aacccgccgc gcccccccgt gggaaaccac ccgagaccca
     1141 cggagacgag caagatgagc agcagcccga ggtagaagcc ggggatcgct tggccgatca
     1201 acaccaggac ctgcgctgtg cggtctgccg gcgaccccgc cgaaaccgcc gcatagacgc
     1261 caccgacgac gcccacgacc acggccacgc cgtacgcgga cgcgccgagt tccagcgtgg
     1321 cgggcagccg ctcgaggaag agctgcagca cgggctcctt gtacctgagc gacgacccaa
     1381 aatttccgtg cgccgaggcc gcgaggtacc gccagaactg ctcccacaca ggcctgtcga
     1441 gcccgtgttc tgctcggaat tcggcgacga cgtccggtgt ggcctcaacg cccagcacga
     1501 atcgcgaagg atcccctgtc acgcgaacga tgaggaaagt caccaggaca atcccaaaga
     1561 tcgtcatgac agcccaaaat ccccggccca ccaagtacgg ccccatccgg actcctctca
     1621 gcttctccca ccggtgctgg ggatcttgga gattggagat cctcggtcgg taaatgtggt
     1681 gccggaggag ggactcgaac ccccacgggc gtgagcccag gcgattttga gtcgccatcg
     1741 tctgccgatt ccgacactcc ggcccggcgt gcctccagta tcgtagcacc cgcggcgacc
     1801 ggcctcaagg gcacgggagg gcttccgggc cgggccggac gcccgtgcta taatcgtccc
     1861 gggatgtctt ccgaccggcc tcttctgatc ctcatcgacg ccaacggcct cgtctaccgc
     1921 gccttcttcg ccctacccta cttcacgacc agcaacggcc agcccaccaa cgccgtgtac
     1981 ggctttgcga cgatgctgct gaaggtcctc gaggaggaat cgcccgacta cgtcgcagtc
     2041 gcgttcgatc gccccggccc caccttccgc cacgaggcgt acgccgagta caaggccacc
     2101 cgccggcaga tgccggacga tctgcggccg cagatcggtc tcgccaagga ggtcgtggaa
     2161 gccctccggc tcccggtgtt cgaagtcgcc gggttcgagg ccgacgatat catcggcgcg
     2221 ctggcccgga aggccgaagc ccgggggatg gacgtcctga tcgtcaccgg ggatctggat
     2281 acgctccagc tcgtctcccc gcatacgcgg gtgatgatga ccgcccgggg gatcaccgag
     2341 accacggtgt acgacgaggc ggcggtgcgg cagcggttcg gcatcgcccc cgcgcagatc
     2401 ccggacctca agagcctgaa gggcgatacc acggacaaca tccccggcgt gcccggggtg
     2461 ggcgagaaga ccgcgtcccg gctgctcgcc cagtacccca gcgtggaagc gttgctcgac
     2521 gcggtggggg agatccgcga gcccaaactc cgccagcagc tcaaggcgcg ccgcgagcag
     2581 gtcctccaga gcaagcacct ggccacgatc gtgaccgatc tcgacgtccc gctcgacccc
     2641 gagttcctgc ggcgccggcc cccggatcag gaccaggtca aggcgctgtt caccgcgctc
     2701 gagttcaaga cgctgctgga gcgcctcggg gtggcggtcc cgaccccgca gggccggggc
     2761 gcctaccgca ccgtgcgccc ggaggcgctg cccgccctgc tggagggcgc ccagcatctc
     2821 gcggtggcgc tcgccgccga cccggggcac ccgctcaccg cgcggctcca cgggatcgcc
     2881 gtgtcggccc ggaccggcga gggggcctac ctggccgggg ccgcgatccc cgcgcccctc
     2941 gcgggcctcc tggagcgcga ggatctgccg aagtacagcc aggacatcaa gcacgaccgc
     3001 ctgctgctcg agcaggcggg gctgcacccc cgggggtttg cgtttgacgt gtccctggcc
     3061 tcgtacctgc tcgatcccgg caagcgcacg cacacgctcg acgccgccgc gtggcagttc
     3121 ctggggtggc ggcttggggc gggacaagcg gaggagggcg ggttggccct gggccaggcc
     3181 cccgacgagg ccaccgccga gcaggcggac gccatcgggc ggctggtgga agtgctggac
     3241 gcccgcctcc gggaacggga cctgctcggc ctctaccgcg agatcgagct gccgctcgtg
     3301 gacgtgctgg ccaggatgga aacggtcggg gtcgcgatcg atgtggaggc gcttcgggcc
     3361 ctctcgggca ccctccgcgc ccggctggag gcgctcacga ccgagatcta ccggctggcc
     3421 ggcatggagt tcaacatcgg ttcccccaag caactggcgt tcgtcctctt cgagaag
//
LOCUS       DASQGH010000300         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9741, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000300 DASQGH010000000
VERSION     DASQGH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9741"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VEW91_05880"
     CDS             <1..1047
                     /locus_tag="VEW91_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM91144.1"
                     /translation="PDRRKLIVVRVDIAAHILPARYFARLQQIPGFYLAKRVKGVPGL
                     FDLNVRFRIMDGFDAYQQVLSLASPPLDRLGSPEVTPDLARLANDELAGLVAAHPDRF
                     AGALGGLPLNNPDASLRELDRLARLPGFVGVQIYSNVAGRPLDEPRTLEVVEEAARRR
                     MAIFLHPARGADFPDYPGEEHSLYDLWQIFGWPYETTIAMARLIFTGLFDRHPDAVII
                     THHMGGLVPHFADRIRGGYDQFGTRGPEHQVETFPVALAHPPAWYFPRFYADTALFGA
                     PHAVRCGLAYFPVERVLFGTDTPFDAEGGTRYIRETIAALDQSGLPPDRRQMVDEGNL
                     RRVLARRQKVSKPP"
     gene            1088..1399
                     /locus_tag="VEW91_05885"
     CDS             1088..1399
                     /locus_tag="VEW91_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91145.1"
                     /translation="MLGPAATGRATAPVRMGYLGVSSDVGIFIALDKGYFKEQGLDLS
                     LERFGVGADQMAHRESPCRRRSGRNAAVRPEVERLPRSAGSGRRRVHLVRLRQVGRHD
                     V"
     gene            complement(1473..1754)
                     /locus_tag="VEW91_05890"
     CDS             complement(1473..1754)
                     /locus_tag="VEW91_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91146.1"
                     /translation="MNRKYAYWNHAALVVGANGEIIEALGPSPGVVQQDVSKYAPREY
                     TIVRIKASPEDRDEAAGFARACRGERYGVFTIVSIAPFPLDRDEVQLRV"
     gene            2132..3400
                     /locus_tag="VEW91_05895"
     CDS             2132..3400
                     /locus_tag="VEW91_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM91147.1"
                     /translation="MNHPSWPSGTREPGIRAGTALAVILAIASGLRIADLGRNSLWFD
                     EAFVVWVTRLGWQNIPPHGDFHPPLYYLLMKAWVGIAGTGEAAIRFPSACFSVVSVGL
                     AYALARRVCSERVGLLTALLVSVSPFQIMAGQDARMYSLLAMLALASTLALVMSIGRG
                     GALRWAGYVVLAALMVYTQYLGFFVLIAHGIWVASYERVHFTRWLAGMVAAGLLYAPW
                     MPSLWYQTVHMRDWTYWPDGAMYRNLLDLLGLYAFGGSLFGMANYFFGGTLGAVEQFI
                     ILLPFLAVLWRGVISLGSERRSLALLGLPPAVTIATMSALYLIKPIFYPRWFSFLSPF
                     YAMFLAQGIVDVAERFRGQRDRSLAFLTAGLLLYSAPVLAHYYFDPGFRPYQWRAAAA
                     LVRREVRPGDFFLYVGQAAEISFTYYFRDP"
     gene            3428..3652
                     /locus_tag="VEW91_05900"
     CDS             3428..3652
                     /locus_tag="VEW91_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91148.1"
                     /translation="MMPQGHRPPAFTEGQVQQLARQYPRVWLIPTAAFYTQTQQRLLP
                     ELNTAFRVAGYRDFFGTWVCLLEAKLPKSR"
     gene            complement(3688..3969)
                     /locus_tag="VEW91_05905"
     CDS             complement(3688..3969)
                     /locus_tag="VEW91_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91149.1"
                     /translation="MLSVLRDSRGTFLAFVPPDAHIPPGQLVRLNTPAGPKLKGRMFL
                     VPIQTAAVLVPVDTLTLVAAHIEDSGPRLTIILVGTPVPNLTVVFGVQL"
     gene            complement(4172..4474)
                     /locus_tag="VEW91_05910"
     CDS             complement(4172..4474)
                     /locus_tag="VEW91_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91150.1"
                     /translation="MIVRHVVGYLVCVAFLTLVLTFVGPFFDLIQASETHVGRIIDVF
                     RFDPDIGPWTSYTVHILGLSFAPVAVLGHVIAVVASPVGLLALMLYFLMAWVMPAR"
     gene            4696..5520
                     /locus_tag="VEW91_05915"
     CDS             4696..5520
                     /locus_tag="VEW91_05915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYM91151.1"
                     /translation="MTVQAEWVQFHSGGAVVPAYMARPARVSRPIPAILVIQEIWGPD
                     EHIQDVARRFGAAGYVAVAPDLYARGGRRPSALAADRIEAVKAFLDSMPPAAWSNPSE
                     RDAALARLPEPQRRQVGETLGLLFSPNRPLEQYTADLRAAVQYLRGHAASQGQRVGSV
                     GFCMGGALSALLACSEPALSAAVIFYGSAPPPERIAGIRCPILGFYGGDDPRITQGVP
                     AFAEALHAAGKAFESHIYPGAPHAFFNDTRGSYRVDPSREAWARTLAFFAQHLAAA"
     gene            complement(5568..5936)
                     /locus_tag="VEW91_05920"
     CDS             complement(5568..5936)
                     /locus_tag="VEW91_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91152.1"
                     /translation="MITSLACSLVGQKQPVTIIPGTRAAALWGAGEVTEAYYCNYGLN
                     PEYRRQLEAAGMVVSGVGGQGEVRIVELPTHPFFIATLFLPQARSTPERPHPLIAGYA
                     AAAAAHRTPRRSEHGARPSS"
BASE COUNT         1018 a         2153 c         2064 g         1156 t
ORIGIN      
        1 ccggacaggc gtaagttgat cgttgtgcgc gttgatattg cagcccacat cctcccggcc
       61 cgttacttcg cccggcttca gcagatcccg ggattctacc tggcgaagcg ggtcaagggg
      121 gtcccaggcc tcttcgacct caacgtgcgg ttccggatca tggacggctt cgacgcgtac
      181 caacaggtgc tgtcgctggc ctccccgccg ctcgaccgcc tcggatctcc agaggtcacg
      241 cccgacctgg cccgcctggc caacgacgag ctcgccggcc tcgtggcggc gcaccctgac
      301 cgcttcgcgg gcgcgctggg aggcctcccg ctcaacaatc ccgacgcaag cctccgcgag
      361 ctggaccggc tcgctcgcct ccctggcttt gtcggcgtcc agatctattc caatgtcgcg
      421 ggacggccgc tcgacgagcc acggacgctc gaggtggtcg aggaggctgc gcggcggcgg
      481 atggcgatct tcctccaccc ggcgcgggga gctgatttcc ccgactaccc gggtgaggag
      541 cattcgctgt acgacctctg gcagatcttc ggctggccgt acgagacaac gatcgccatg
      601 gctcgcttga tcttcaccgg gctcttcgac cgccatcccg acgcggtgat catcacccac
      661 cacatgggtg gcctggtccc gcacttcgcc gaccggatcc ggggcgggta cgaccagttc
      721 ggcacccggg gaccggagca tcaggtggaa acgttccccg ttgccctcgc ccacccgccg
      781 gcctggtact ttccccgctt ctacgctgac acggcgctgt tcggggcccc ccacgccgtc
      841 cggtgcggcc tggcctactt tccagtcgag cgggtgctct tcggaaccga caccccgttc
      901 gacgccgagg gagggacccg atacatacgc gagacgatcg cagcgctcga tcagagcggc
      961 ctccctccgg atcgccggca gatggtcgac gagggcaacc tccggcgggt gctcgcccgc
     1021 cgccagaaag tgagcaagcc tccgtgacac gcccttgcgc gacgctcgtg ctgggcttgg
     1081 cctcgggttg ctgggcccgg cagccaccgg gcgggccacc gcccccgtcc ggatgggata
     1141 cctcggcgtc tcgagcgacg tcggaatctt catcgccctc gacaaagggt acttcaaaga
     1201 gcaaggcctg gatctctcgc tcgagcggtt tggggtcggc gcggaccaga tggcgcatcg
     1261 ggagagcccg tgccgcagac ggtcgggccg taacgcggcg gtccgccccg aggtggagcg
     1321 cttgcccagg tccgccggtt cagggcgccg gagggttcac ctggtacgcc ttcgccaggt
     1381 cggccggcat gatgtgtgac ggctccttcg tgaagatcgc acgcgtcctc tccaaggccc
     1441 gggcgacgag gcccgagcag atcatctgcc cattaaaccc gaagctgaac ctcgtcccgg
     1501 tcaagaggga aaggggcaat gctgacaatg gtgaacacac cataccgctc cccgcggcag
     1561 gcccgggcga agccggccgc ttcatcccga tcctccggcg atgccttgat ccggacaatg
     1621 gtgtactcgc ggggagcata cttcgagacg tcctgctgga cgactcccgg tgaaggccca
     1681 agcgcctcga tgatttcgcc gttggcgccc acgacaagcg cggcgtgatt ccagtacgcg
     1741 tacttccgat tcatcccgcg gaacctcagg gcctgcccca ctctgatcag agaactggtc
     1801 caatgacgtc cgtgggtcaa gatgaagtcc cccggtcgca tcgcggccgc tgtggggacg
     1861 gcccctgatc ttcccgggcc cgcgcgcaca tgctggaatc cctcgccttc tgccagttcc
     1921 cttaccatga gaatccttca ccccccatgg gcttcgattc tcccctcctg cgggacgggg
     1981 cgccggaggg tgcattcccg aagttttcgg cccaactcta acgcacgatc gcgtccgatg
     2041 caaccggcta aacctcggca atgttgtgca tttggtcctc agaaaggggc gggcggaggc
     2101 tgttctgacc ccgtgccggc tggatccacc gatgaatcat ccttcgtggc cctccgggac
     2161 gcgcgagccg ggtatccgcg cgggaaccgc cctggccgtc atcctcgcca tcgcgtccgg
     2221 acttcgtatt gcggacctgg gccggaacag cctgtggttc gacgaggcgt tcgtcgtatg
     2281 ggtcacgcgt ctcggatggc agaacatccc gccccatggc gactttcatc ccccgctgta
     2341 ctacctcctg atgaaggcct gggtgggaat cgccggcacc ggggaggcgg cgatccggtt
     2401 tccctcggcg tgcttcagcg tggtctccgt cggcctggcc tatgccctgg cgcgccgggt
     2461 ctgctcggag cgcgtcggcc tgctgaccgc cttgctcgtc agcgtctcgc ccttccagat
     2521 catggcgggg caggacgcca gaatgtactc ccttctcgcg atgctcgccc tggcctcgac
     2581 gctggcgctc gtcatgagca tcggtcgggg tggcgcgctc cgctgggcgg ggtatgtggt
     2641 gctggcagcg ctgatggtgt atacgcagta tctgggcttc ttcgtgctca tcgcccacgg
     2701 catctgggtg gccagctacg aacgcgttca cttcacgcgg tggctggccg gcatggtggc
     2761 cgccggcctc ctgtatgctc cgtggatgcc ctcgctctgg taccagacgg tccacatgcg
     2821 agactggacc tattggccgg acggcgcgat gtaccgcaac ctgctcgatc tcctcggcct
     2881 ctacgcgttt gggggctcac tgtttgggat ggcgaactac ttcttcggcg gcacgctcgg
     2941 ggcggtcgag cagttcatca tcctcctgcc gtttctcgcc gtgctctggc ggggcgtcat
     3001 ctcgctcggg tcggagaggc gcagcctggc gcttctcggg cttccgcccg ccgtcaccat
     3061 cgcgaccatg tccgccctct accttatcaa gcccatcttc tatcctcgct ggttctcgtt
     3121 cttatccccg ttctatgcca tgttcctcgc tcaaggcatt gtcgatgtgg cggagcgttt
     3181 tcgcggacag cgggaccgga gcctcgcatt tctcacggcg ggcctgctcc tgtacagcgc
     3241 gccggttctc gctcattact acttcgaccc ggggttccgg ccctatcagt ggcgtgcagc
     3301 cgccgcgctg gtcaggcggg aggtcaggcc cggagatttc ttcctgtacg tgggccaggc
     3361 ggccgaaatt tccttcacgt actatttccg ggacccataa ccgtcgctga cgctgacccc
     3421 gaccgaaatg atgccgcagg gacatcgtcc tccggcgttt acggaaggcc aggtgcagca
     3481 actggcccgg caatacccgc gggtgtggct catccccacc gcggcgttct atacgcagac
     3541 gcagcagcgg ctcctcccgg agctgaacac ggcatttcgc gtcgccgggt accgcgattt
     3601 cttcgggacc tgggtatgtc tgctcgaagc gaaactgcca aagtcccgct gaccgatctg
     3661 gtcccccctc agcggccagt tacaccctca caactgcaca ccgaagacca cggtcaaatt
     3721 ggggacaggc gtgccgacca gaatgattgt caaccttgga ccggaatcct cgatgtgcgc
     3781 cgccacaagg gtgagggtgt ctaccggcac caacaccgca gcggtctgga tcgggacgag
     3841 gaacatgcgc cctttcagct tgggcccggc gggggtgttg aggcggacca gttgccccgg
     3901 gggaatatgc gcgtctgggg gaacgaacgc caggaacgtc ccccggctat cccgcaggac
     3961 tgagagcacg taacactgtc cggctgggat gacgattttc ccgtaaacaa gcggagcggc
     4021 agggcagaat ggctggcctt cgctgcgagc acaaaggcag agactcgtct tgtcactgcc
     4081 atccctcccg attaattcgt gacgccgttt ggcggcgcgg cgtcggatgc ggtcccccgg
     4141 atagtcgcca gggggtgatg tcacctgtcc ctcatcgggc tggcatcacc caggccatga
     4201 ggaagtagag catgagggcg aggaggccga ccggcgaggc cacgacggcg ataacgtgcc
     4261 ccagaacggc caccggagcg aacgacagcc caagaatgtg aaccgtgtag ctggtccaag
     4321 ggccaatgtc cgggtcgaat cgaaaaacgt cgataatcct cccaacgtgc gtctcgctgg
     4381 cctggatcag atcgaagaac gggcccacaa aggtcaagac aagcgtcagg aaggcgacgc
     4441 atacgaggta cccgaccacg tgtcgcacga tcaccaccaa cttctggggg ggcggcttga
     4501 cctccggccc acgctatacc ctatacccta tgccctgtat cctccggttg cagacagccc
     4561 gcccggcccg gggggaatgg gagggggtgg cgcagcaggt tacggccttc gtaccgctga
     4621 acaacgtccg gcacggcagg ttctccaccg caacttcacc gcgtcggtca ggctcgtgag
     4681 aggagaagcg cgtcgatgac ggtccaggct gaatgggtgc agttccactc cggtggggcg
     4741 gtcgtaccgg cttacatggc ccggccggcg cgggtatcca ggccgattcc agccatcctg
     4801 gtcattcagg agatctgggg accggacgaa cacatccagg acgtggcccg caggtttggt
     4861 gccgccgggt acgtagccgt ggcgcccgat ctttatgcga ggggaggccg gcgaccatcc
     4921 gccctggccg cggaccgtat tgaggccgtt aaggcgttcc tggattcgat gccgccggcg
     4981 gcctggtcaa acccctctga acgggacgcg gcgctggcgc gcctgcccga gccccagcgg
     5041 cgccaggtcg gggagacgct aggactcctg ttttctccga atcgaccgct tgagcagtac
     5101 accgctgatc tccgcgcggc cgtccagtac ctgcggggac acgcggcgtc ccagggacag
     5161 cgcgttgggt cggtagggtt ttgcatgggg ggcgcgcttt ccgcgctgct ggcgtgctcg
     5221 gagccggcac tctccgcagc cgtcatcttt tatggaagcg ccccgccgcc agagcggatc
     5281 gcggggatcc ggtgtccgat cctcggcttc tacggcggcg atgacccgcg catcacgcag
     5341 ggtgttcctg ccttcgccga ggcccttcac gccgccggca aggcgttcga gtcccacatc
     5401 taccctgggg cgccgcacgc gtttttcaat gatacgcggg gatcctatcg cgtcgatccc
     5461 tcccgggagg cttgggcgcg cacactcgcc ttcttcgcgc agcacctggc cgcggcctga
     5521 tgccggggag atcgagagcg cttgtcggcg aagctcctca tccaccgtca actggaaggg
     5581 cgtgctccgt gctccgaacg gcgcggtgtg cggtgcgcag ccgcagcggc cgcgtagccg
     5641 gcaatgagcg ggtgcgggcg ttccggggtt gagcgcgcct ggggcaagaa cagggtggct
     5701 atgaagaacg ggtgggtcgg cagttcgaca atccgtacct ccccctgccc tcccacgccg
     5761 ctgacgacca tcccggccgc ctcgagttgt cggcggtact cgggattgag cccgtagttg
     5821 cagtagtagg cttcggtcac ctccccggcg ccccacagcg cggccgcgcg cgtgccgggg
     5881 atgatggtga ccggctgctt ctgtccgacg agcgaacatg caagggacgt gatcaacagg
     5941 cacgtccccg ccgggttggt ctctgcatga tcggcgtcct cgagcccgag gacgttgcgc
     6001 gcgaactcga cgatcgcgtg ctgaaagcct cctcaggtcc cgaccagcgg tacgccgcgc
     6061 tcgcgcgcgt agcggatggc attcaacgcc ccgtccatgc tgcggtaggg actcgcagga
     6121 gcgatccaga gtccgtcaaa ggacgcgagc cgatcatccg gggtgccgcc gacgcgttct
     6181 gttggtaccc actcgcagtc gaggcccgca tgggccaggg cctcatttgt gaatcgatgc
     6241 gtgggattgc cctcagcgaa gtcgccgacg accccgatca gggggcgaac catcctggcc
     6301 acggaggcag tataccacgc tggctacggg cctaccgacg cttaaccggc tcccagtgag
     6361 ccagatgagg gatctggagg taagccgggg a
//
LOCUS       DASQGH010000301         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1299, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000301 DASQGH010000000
VERSION     DASQGH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VEW91_05925"
     CDS             complement(<1..663)
                     /locus_tag="VEW91_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91153.1"
                     /translation="MTVPPVLELEGICRRFGGLDAIKDVSLSIPAGTRRAIIGPNGAG
                     KTTLFNIITGELGPTSGRLRLFGKDVTRLPAYARAAMGLGRTFQITNLFGPLTVEENV
                     LLAAQALSPSKYAMTRPLSRDVAVRNRARALLEPAGLWAKRDVEVRALSYGEQRQLEV
                     LLALAGRPRLLLLDEPAAGLSPGERREMARFIKGLDPQLTMLLIEHDMDVAFEVVETI
                     TVL"
     gene            complement(697..2013)
                     /locus_tag="VEW91_05930"
     CDS             complement(697..2013)
                     /locus_tag="VEW91_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91154.1"
                     /translation="MAQRITRRMVLGGGAALAGVAAAGALSDVRSIAYAQGGPPPIRI
                     GFLPALTGTAAPSGRDMLDGLTLYLSQRNSTIAGRPVQLIVEDTGGNPTNALTKTRKL
                     VEGDRVHALIGPLLANEGYAIRDYVIERKVPTLLPVVSADDLTQRKRSPYLIRTGWSS
                     GQPSHPFGEYAYATLKYRKIAMLGNDYAFAYEVNGGFQQTFEAHGGQIVQKLWAPLGT
                     PDFSPYVTQLRRDVDAIYATTVGADSIRFVHAYRQYGLKDRIPLIGGGTLMDESLLRG
                     MVAEDAVGVVTALHWSAGLRTPEARRFVSAYVAKYQKDPSYYAETCYTCGRWIDVATA
                     HIGGKVEDRDAFLQALRTLQLPDAPRGPVRLDAYQNPIENVYVRKVEVAGSAAPSETF
                     AAQTGHLLNSVIYTFHNVSQFWTFSPEEYLKQPVYDRNYPPCKFCG"
     gene            complement(2040..>2640)
                     /locus_tag="VEW91_05935"
     CDS             complement(2040..>2640)
                     /locus_tag="VEW91_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM91155.1"
                     /translation="RGIPRPLLGFGLNLGRIQEFYYFALAATVVATGLMYLLVRSPFG
                     LSLRGIRESESRMRVLGYDTFLHKYLIFVIAGLFSGVAGALYVYYNGFVSPSDVNLVA
                     SAYALLMVILGGTGTLFGSLVGSGLLVFLQNLLSAKTQRWLTVLGVILVLSVMYAPLG
                     IVGTARTILTRATSRPRAQAGAASVPEPSESGEPVGPNS"
BASE COUNT          444 a          906 c          883 g          407 t
ORIGIN      
        1 cagcacggtg atcgtctcga caacttcgaa cgccacgtcc atatcgtgct cgatcagcag
       61 catcgtgagc tggggatcca gccctttaat gaaacgcgcc atctcccggc gctctcccgg
      121 cgacagcccg gcggccggct cgtcgaggag caacagacgc ggacgcccgg ccagcgcaag
      181 cagcacctcg agctgccgct gctccccgta ggagagcgcc cggacctcca cgtcccgctt
      241 ggcccacagc cccgcgggct ccagcagggc gcgcgcgcgg ttccggacgg cgacatcgcg
      301 gctcagcggg cgggtcatcg cgtacttgga tggggacagg gcctgcgccg cgaggagcac
      361 gttttcttcg acggtgaggg gcccgaagag gttggtgatc tggaacgtcc gccccagtcc
      421 catcgccgcg cgcgcgtagg ccggcaggcg cgttacgtcc ttgccgaaca gccggaggcg
      481 gcccgaggtc ggcccaagct ctccggtgat gatgttgaac agggtcgtct tgccggcgcc
      541 gttcggcccg atgatcgcgc gccgcgtccc ggccgggatc gacaatgaca catccttgat
      601 cgcgtccaat ccgccgaacc gccggcagat cccttccagc tcgaggaccg gcgggactgt
      661 caccgcgggg cgcctcctct ccagctcccg cccgccttat ccgcagaact tgcaaggcgg
      721 gtagttccgg tcgtacaccg gctgcttcag gtactcctcg gggctgaatg tccagaactg
      781 cgacacgttg tggaacgtgt aaatcacaga gttgaggaga tgcccggttt gagccgcgaa
      841 ggtctcggag ggtgccgcac tgcctgcgac ctccaccttg cgcacgtaga cgttctcgat
      901 cgggttctga tacgcatcga gccggaccgg cccgcggggg gcatccggga gctgcagggt
      961 ccggagcgcc tgcaggaacg cgtcgcggtc ctcgaccttc cccccgatat gggcggtcgc
     1021 gacatcgatc cagcgaccgc acgtataaca ggtctcggcg tagtacgacg gatccttctg
     1081 atatttcgcg acgtaggcgg agacgaacct tctcgcctcg ggcgtgcgca ggcccgcgct
     1141 ccagtgcagc gccgtgacga cccccaccgc atcctccgcc accatgcccc ggagcaggct
     1201 ctcgtccatc agggtgcccc cgccgatcag cgggatccga tccttgagcc cgtactggcg
     1261 gtaggcatgg acgaagcgga tcgaatcggc gcccacggtc gtcgcgtaga tggcgtccac
     1321 gtcgcggcgc aactgcgtga cgtaggggct gaaatccggc gtgccgagcg gcgcccagag
     1381 cttctgcacg atttgcccgc cgtgcgcctc gaacgtctgc tggaagcccc cgttgacctc
     1441 ataggcgaac gcgtagtcgt tcccgagcat ggcgatcttc cggtacttca gcgtcgcgta
     1501 cgcgtattct ccgaacggat gggagggctg gccgctcgac cagccggtcc ggatcaggta
     1561 cgggctgcgc ttccgctgcg tcaggtcgtc ggcggagacg acgggcaaga gggtggggac
     1621 cttgcgctcg atcacgtaat cccggatcgc gtacccttcg ttggcgagca gcgggccgat
     1681 caacgcgtgc acgcgatcgc cctcgaccag cttccgcgtc ttcgtgagcg cgttggtggg
     1741 gttgccgccc gtatcttcga cgatcagttg caccgggcgg ccggcgatcg tcgagttgcg
     1801 ctgggacagg tagagggtca ggccgtccag catgtcccgg ccgctcgggg ccgcggtccc
     1861 cgtgagcgcc gggaggaacc cgatgcggat gggcggtggc ccgccctgcg cgtaggcgat
     1921 cgatcggacg tcgctcaacg cgccggccgc cgccacaccg gcaagggccg caccgccccc
     1981 gagcaccatc cgacgcgtga tgcgctgtgc catctccccc accccctagg cgaatcgcct
     2041 cacgaattgg gcccgacagg ctcaccgctc tcggacggct ccgggaccga cgccgcgccg
     2101 gcctgggcgc gagggcggga cgtcgcgcgc gtcaggatcg tccgcgccgt gccgacgatt
     2161 ccgagcggag cgtacatcac cgacagcacg aggatcacgc cgaggaccgt gagccagcgc
     2221 tgcgttttgg cgctgaggag attttgcaga aacaccagca gcccggagcc cacgagcgat
     2281 ccgaacagcg tccccgtccc ccccaggatc accatcagca acgcataggc ggacgccacg
     2341 agattgacat cggagggtga gacgaagccg ttgtaataga cgtagagcgc tccggcgacg
     2401 cccgagaaca acccggcgat cacgaagatc aggtacttgt ggaggaacgt gtcgtagccg
     2461 aggacccgca tgcgggactc gctttcgcga atcccgcgga ggctcaggcc gaacggggac
     2521 ctgacgagca gatacatgag cccggtggcg accaccgtcg cggcgagcgc gaagtaatag
     2581 aattcctgga tccgcccgag gttgagcccg aaccccagga gcggccgcgg gatcccgcgg
//
LOCUS       DASQGH010000302         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43746, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000302 DASQGH010000000
VERSION     DASQGH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43746"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..227)
                     /locus_tag="VEW91_05940"
     CDS             complement(30..227)
                     /locus_tag="VEW91_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91156.1"
                     /translation="MELREYVHKLTDIIWDREHRTQKPADAAILSYVQDAVIAELEYR
                     RRMSDLDRRIAELEELTGVHS"
     gene            408..1508
                     /locus_tag="VEW91_05945"
     CDS             408..1508
                     /locus_tag="VEW91_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HYM91157.1"
                     /translation="MRAAILYEVGKPLAVEPVELQEPRPGEVAVRIAATGVCHSDLHY
                     IKGDLGYPLPVVLGHEAAGVVEAVGAGVDSVRPGDHVVLLFAPACGRCRYCDAGRPHL
                     CEMRYRVRGKMPDGTTRLRWRGQDLHHFTCVSSWAERAVVPESGVLRISEEVPFTVAA
                     LLGCAVTTGVGAVINTARVEVGASVAVFGAGGVGINVVQGARIAGAAVIIAVDLLEHR
                     LEAARRFGATHTVNAREEDPVRMIQSLTRGGADYAFEVIGRPAVVRQAVECLARGGVA
                     VAIGLPPAQAEYTVGGPAFVLNEKTLRACFYGSSHLRADIPRLLELYYGKRLLLDELV
                     TATYPLDRVNDAVAALDRGDGLRGLLVMEHAR"
     gene            1508..2761
                     /locus_tag="VEW91_05950"
     CDS             1508..2761
                     /locus_tag="VEW91_05950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYM91158.1"
                     /translation="MRSRARWIGVALCLVAVSAPPPGAPRVEAGVSAAMVRGLAPARE
                     AHPAGPPSSVLMEASTGQMLASIDPHRRLPPASLNKLMTFYLALEAIKSHRLALTTSI
                     TVSEDAWRIGRSPGGSRMFLNVGDTVTVEQLLEGLMVASGNDAAAALAEALAGSDEQF
                     VEEMNAAAAHLGMRDTHYVTPHGLPSPGEYTSAWDMGVLAREILVTFPEAVAYSSPRY
                     ATYGGIRQANWNNLVFRDPRVDGLKTGFTDEAGFDVVATARQDSMRLIAVVMGARTLQ
                     RRTAVAEELLNLGFARYVLVAVPWQQVVPAGLPVYGGTARLLPLETPKPIGVLLSRDD
                     HAPLTVSEEVRVAPVAPFQRGQQVGTLTVRNQTAVLVAAPLLASSTINRAWVLGRVWG
                     MVRYAIWRVFHHRQAVRTGTFTPPP"
     gene            2810..3409
                     /locus_tag="VEW91_05955"
     CDS             2810..3409
                     /locus_tag="VEW91_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HYM91159.1"
                     /translation="MEEGFEVESRLEVSAEASGHWLINAIAGTTAVLAVCAALTSLLA
                     GRAAHHSLAELNQAAIAQNLASDQWNFYQAEGIKRHVFEVQRDVLQLQSGPGVGAQTA
                     AYDKEAKRYAAMQAQIRKDAESREHERDGATAAAQRFDIRYQRLSLAVASFQIGIVVC
                     SVAAIVRGRPLWYLGLAGGAVGMIVLAQELFPMTGGRGG"
BASE COUNT          496 a         1109 c         1274 g          568 t
ORIGIN      
        1 gtacccgggt gtgccttacc gggagtcgat caggaatgaa ccccggtcag ttcttcgagt
       61 tcggcgatcc tgcggtccag gtcggacatt cgccgccggt actccagttc ggcgatgacc
      121 gcgtcctgaa cataactgag gatggccgcg tcggccggct tctgggtcct gtgctcgcgg
      181 tcccagatga tgtcggtcaa cttatgaaca tactcccgca gttccatgcg tctcctcccg
      241 tcccgttcgg ggtactggaa tccttgatgg gggtttaccg agacggaatg attcgcaggg
      301 tgaccgggga tctcctgcaa tcttgcggga aacttcgcga cggcaccgga ggaatcccgg
      361 aacgaaggcg gaagagtgca tcgaggggat cgggagagga gtgaatcatg cgcgccgcga
      421 ttttgtacga ggtgggcaag ccgctcgccg tcgagcccgt ggagttgcag gaaccgcggc
      481 ccggcgaggt cgcggtccgg atcgctgcga ccggggtgtg ccatagcgac ctccactaca
      541 tcaaagggga cctggggtat ccgctcccgg tggtcttggg gcacgaagcc gcgggcgtgg
      601 tggaggccgt cggcgcaggg gtggactccg tccgtcccgg cgatcacgtg gtgctgctgt
      661 tcgctccagc ctgcggccgg tgccggtact gcgatgccgg gcggccgcac ctctgcgaga
      721 tgcgataccg ggtccgggga aaaatgcccg acggcaccac gcggcttcgg tggcgggggc
      781 aggacctgca ccactttacc tgtgtctctt cgtgggcgga gagggcggtg gtccccgagt
      841 ccggcgtgct gcgcatcagc gaagaggtgc cgttcaccgt ggcggccctg ctggggtgcg
      901 cggtgacgac aggggtcgga gcggtgatca acaccgcccg ggtagaggtc ggcgccagcg
      961 tggccgtgtt tggggccggg ggcgtgggga tcaacgtggt gcagggggcg cgcatcgccg
     1021 gcgccgccgt gatcatcgcc gtcgacctgc tggagcaccg gctcgaggcc gcccgccggt
     1081 tcggtgccac ccacacggtc aatgcgcggg aggaggaccc ggtgcggatg attcagagcc
     1141 tgacccgggg cggggccgac tatgccttcg aggtgatcgg gcggcccgcc gtcgtccggc
     1201 aggcggtgga gtgtctggcc cgggggggcg tcgcggtcgc catcgggctg ccgccggccc
     1261 aggccgagta cacggtcggc ggcccggcgt tcgtgctcaa cgagaaaacc cttcgcgcct
     1321 gcttctacgg ctcttcgcac ctccgggccg atatcccccg tctgctcgag ctctactacg
     1381 ggaaacggct gctgctcgac gagctggtga cggcgacgta tcccctggat cgggtcaacg
     1441 acgcggtggc cgcgctcgac cgtggggatg ggttgcgcgg cctcctggtg atggagcacg
     1501 cccgctgatg cgaagccgcg ctcgctggat cggcgtggcc ctgtgcctcg tcgcggtgtc
     1561 ggcgcctccc cccggcgcgc cgcgcgtgga ggcgggggtg agcgcggcga tggtgcgcgg
     1621 cctggccccc gcccgcgagg cccaccccgc cggcccgccc tcctccgtgc tgatggaggc
     1681 ctcgacgggg cagatgctgg cctccatcga tcctcaccgg cgcctccccc cggcgagcct
     1741 caacaagctc atgaccttct acctggccct cgaggcgatc aagagccacc gcctcgcgct
     1801 cacgacctcg atcacggtca gcgaagacgc ctggcggatt gggcgctcgc cgggcgggag
     1861 ccggatgttc ctgaatgttg gcgatacggt gacggtcgag cagctgctcg agggcttgat
     1921 ggtggcctcg ggcaacgatg cggcggcggc cctcgccgag gccctggccg ggtcggacga
     1981 gcagtttgtg gaggagatga acgcggcggc cgcgcacctc gggatgcgcg acacacacta
     2041 tgtgaccccc cacgggcttc cgagcccggg agagtacacc agcgcctggg atatgggagt
     2101 gctggcccgc gagatcctgg tgaccttccc ggaggcggtc gcctacagca gcccccgcta
     2161 cgcgacctac ggcgggatcc gtcaggcgaa ctggaacaac ctggtgttcc gcgatccccg
     2221 cgtcgacggt ctgaagaccg gcttcaccga tgaggcgggg tttgacgtcg tggcgacggc
     2281 gcggcaagat tcgatgcggc tcatcgccgt ggtcatgggc gcgcgcacgc tgcagcggcg
     2341 gaccgcggtc gcggaggaac tgctcaacct ggggtttgcc cggtacgtgc tggtcgctgt
     2401 gccgtggcaa caggtggtcc ccgcgggtct cccggtgtat ggggggaccg cgcggctcct
     2461 cccgctcgag acgccgaagc ccatcggggt gctgctctcg cgcgacgacc atgccccgct
     2521 caccgtctcg gaggaggtcc gcgtcgctcc ggtggctccc ttccaacggg gtcagcaggt
     2581 gggcacgctc accgtccgca atcagaccgc agtgctcgtg gcagcgccgt tgctggcgtc
     2641 ctcgacgatc aaccgcgcgt gggtgcttgg ccgggtctgg ggaatggtcc ggtacgcgat
     2701 ctggagagtc ttccaccacc gtcaggcggt ccggaccggc acgttcactc cgccgccgta
     2761 gcaagaccag cgcaggagag cccacgcgcg atcccgaacc ggacaggtca tggaagaggg
     2821 gtttgaagtc gagtcccgct tggaagtgtc cgcggaggcc tcggggcact ggctgatcaa
     2881 tgccatcgcc gggaccaccg cggtgctcgc ggtctgcgcc gccctgacca gcctgctggc
     2941 cggccgcgcg gcgcatcatt ccctggcaga gttgaatcag gctgcgatcg cgcagaacct
     3001 ggcctcggac cagtggaact tctatcaggc cgaggggatc aagcggcacg tgtttgaggt
     3061 gcagcgggac gtgttgcagc tgcagtcggg gccgggggtc ggcgcgcaga cggcggccta
     3121 cgataaggaa gccaagcggt acgccgccat gcaggcgcag atcaggaagg atgccgagag
     3181 ccgcgagcac gaacgggatg gagccacggc ggcggcccag cgttttgaca tccgctatca
     3241 gcgcctgtcc ctggcggtcg cgtcctttca gatcggcatc gtcgtctgct cggtggccgc
     3301 gatcgtccgg ggacggccgc tctggtacct ggggctggcc ggcggtgcgg tggggatgat
     3361 cgttctcgct caggaactct ttccgatgac gggaggccgg ggcgggtgag ggttactcca
     3421 ggtcgatccg ggggaccgcc gggccgg
//
LOCUS       DASQGH010000303         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46679, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000303 DASQGH010000000
VERSION     DASQGH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46679"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VEW91_05960"
     CDS             <1..699
                     /locus_tag="VEW91_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYM91160.1"
                     /translation="CLMGILFCHEMGHKLVSIWRGIDASLPFFIPMPPLAGVGIGTMG
                     AVIVTRTPAPNRDALVELGASGPIAGFLVAIPVLVYGIAHSAVIAHSAVLANTCQIST
                     PRLMDLLTQWLLHPGPSADVILHPVAFAGWVGLLVTALNLLPGGMLDGGHVMRAVFGA
                     RAHLVLSIAAAGVAFLFNWWLMGLLILVLVRRGHPGPLDDCSPVTPGRLAMAGVLAVI
                     FALSASPLTLSCHP"
     gene            728..1450
                     /locus_tag="VEW91_05965"
     CDS             728..1450
                     /locus_tag="VEW91_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91161.1"
                     /translation="MVEAQDLQKRFRTFHAVKGVSFRCYAGEVFGLLGPNGAGKTTTI
                     RMLTTILRPTSGTARIAGHDVIEDPEAVRRVIGVLPENTGIYGRLTAREAVRYAGRLY
                     GVQPEVLEARVEAIFDELELTPDIDRVTDTFSKGMKQKVNIARALVHDPPVVFLDEPT
                     SGLDVISARSVREFVQRFKRDGRCVIMSTHIMEEAERLCDRVAIIAGGKVQAEGRLDD
                     LKARTGLGLEETFVQLVEDTVR"
     gene            1447..2709
                     /locus_tag="VEW91_05970"
     CDS             1447..2709
                     /locus_tag="VEW91_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM91162.1"
                     /translation="MILRALAWVRRRLGGESARGPAPQPPGPYVRAVYAKEMLEAVRD
                     RRTLLVMILLPAIVMPIATLGIPYLEQRQQRVLKTSTPSVAIVGQASGLVHLAYATKL
                     VRPVRTNDPAKALRERRVLAVLRVPPGMEASIAQDAQVHVTIQYDASDSESTAARDKI
                     VALISRYSQQVVARRLVARRLNPADLLPVVLDERNVATERQLSGLLLAGLLPFFIAMW
                     AVVGGMSVAVDLAAGEKERGTLESLLVTPPSREAIVVGKFLAVLTASVGAVAIVVTTM
                     MLSLHWGYPYLLHTSKKLDVTLPLGTAVVLFGVALLFSALVSAIQLAVSIYARSPREA
                     QQYVTPLYFAIVLPALAVQFISEWQGTLWVYLVPVLNTFFAFRELLLGTVNWAHLALV
                     AVSCVFYATAVLDLAVAFFSRESVIFRT"
     gene            2886..3410
                     /gene="nrdR"
                     /locus_tag="VEW91_05975"
     CDS             2886..3410
                     /gene="nrdR"
                     /locus_tag="VEW91_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HYM91163.1"
                     /translation="MRCPYCSGEDSRVLDSRGIDEGEAIRRRRECLECRRRFTTFERA
                     ERLVLHVIKRDGRREAFDRAKVLTGMLRACEGRPVPREVLEQTAGEIERELLEGGAAD
                     VSSREIGDRVIERLRRMDDVAYVRFASVYRRVGDVDRLVEEIQRLKARKQLEAELQAQ
                     IELIPLLPQNPGQN"
     gene            3516..>4472
                     /locus_tag="VEW91_05980"
     CDS             3516..>4472
                     /locus_tag="VEW91_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HYM91164.1"
                     /translation="MSVSKETLQFFSGDELRARVFSDKYALRDLDGQILERTPVDMWR
                     RVADGVASVEPTEEARERYARMFYWLLRDFRFIPGGRIMHAVGNPKRVTALNCFPAGT
                     RVLTREGFKVIEEIRAGDEVLTHRNRFRSVTHTMHREAAEPLRTVRLWYLGDRQIAAT
                     KEHRFLAYDGSRVDWVEAQHLTPSHYVKAGRIDETLPVRELDLSEYVSAAAVEDEVGK
                     LYTVTSFVGGQGARRRIESKRVSRTVPVDERFGLWLGFFMAEGGITDNSVYFTFNKNE
                     EAYAESIYNLTQQLFGVEAAIQRREGQEGQWLRVYVHSKMMVE"
BASE COUNT          674 a         1480 c         1553 g          765 t
ORIGIN      
        1 tgcctcatgg gcatcctgtt ctgccacgag atggggcaca agctggtctc gatctggcgg
       61 gggatcgacg cgagcctgcc gttcttcatc cctatgcccc cgctggccgg ggttgggatc
      121 gggacgatgg gggcggtgat cgtgacccgg acccccgcgc ccaaccggga cgcgctcgtg
      181 gaactcggcg cctcagggcc gattgccggg ttcctcgtgg ccatccccgt gctcgtctac
      241 ggcatcgcgc actcagcggt gatcgcgcac tcggccgtgc tcgccaacac ctgccagatc
      301 tcgaccccgc ggctcatgga cctgctcacc cagtggctcc tgcacccggg gccgtcggcg
      361 gatgtcatcc ttcacccggt ggcctttgcc ggctgggtgg gcctgctcgt gaccgcgctc
      421 aatctgctgc cgggtgggat gctggacggc gggcacgtca tgcgggcggt gttcggggcg
      481 cgggcccatc tcgtcctctc catcgccgcg gcgggtgtgg cgttcctgtt caactggtgg
      541 ctcatgggcc tgctgatcct ggtgctggtg cgccggggcc accccggccc gctggacgac
      601 tgctcccccg tgacgcccgg ccgcctggcg atggcggggg tcctggccgt gatcttcgcc
      661 ctcagcgcct cgcccctcac cttgtcgtgc cacccgtgat cggcggttcg tgaccacgcc
      721 cccggtgctg gtcgaggcgc aggacctcca gaaacgcttc cgcaccttcc acgcggtcaa
      781 gggcgtgtcg ttccggtgct acgccggcga ggtgtttggg ctgctcgggc caaacggcgc
      841 cggcaagacc accacgatcc gcatgctcac cacgatcctc cgtccgacct ccggcacggc
      901 ccggatcgcg ggccacgacg tcatcgagga tcccgaagcc gtccgccggg tgatcggcgt
      961 gctgccggag aacacgggga tctacggccg cctcaccgcc cgggaagcgg tgcgctacgc
     1021 gggccggctg tacggagtgc agccggaggt gctcgaggcc cgggtggagg ccatcttcga
     1081 cgaactcgag ctgaccccgg acatcgatcg cgtcaccgac accttcagca agggcatgaa
     1141 gcaaaaggtc aacatcgccc gcgcgctcgt ccacgatccg cccgtcgtct tcctcgacga
     1201 gccgacctcc ggcctggatg tcatttcggc gcggtccgtc cgggagttcg tgcagcggtt
     1261 caagcgggac ggccggtgcg tgatcatgtc gacccacatc atggaggagg cggagcggct
     1321 ctgcgaccgg gtggcgatca tcgccggggg gaaggtccag gccgagggcc ggctggacga
     1381 cctcaaggcc cggacggggc tcggcctgga ggagaccttc gtccagctcg tggaggacac
     1441 cgtccgatga tcctccgggc tctggcctgg gtgcggcggc gcctgggggg cgaatccgcg
     1501 cgggggccgg cgccccagcc cccgggaccg tacgtgcggg cggtctacgc caaggagatg
     1561 ctggaggccg ttcgggaccg gcgcacgctc ttggtgatga tcctgctccc ggccatcgtg
     1621 atgccgatcg cgacgctggg gattccctac ctggagcagc ggcagcagcg cgtgctcaag
     1681 acctcgaccc cctcggtggc gatcgtcggg caggcgagcg ggctggtgca cctcgcctac
     1741 gccaccaagc tcgtgcgccc ggtccgcacc aacgatccgg ccaaggcgct gcgcgagcgg
     1801 cgggtgctgg ccgtgctccg ggtgcccccc ggcatggaag cgagcatcgc gcaggacgcc
     1861 caggtccacg tcacgatcca gtacgacgcc agcgactcgg agagcaccgc cgcccgggac
     1921 aagatcgtgg ccctgatttc ccggtacagc cagcaggtcg tcgcgcgccg gctggtggcc
     1981 cgccgcctga accccgccga ccttctcccg gtcgtcctgg acgagcgcaa cgtagccacc
     2041 gaacgccagc tctccggcct cttgctcgcc gggctcctgc cgttcttcat cgcgatgtgg
     2101 gcggtcgtgg gcggcatgtc ggtcgcggtc gacctggccg ccggggagaa ggagcgcggc
     2161 acgctcgagt ccctgctcgt cacccctccc agccgggagg cgatcgtggt ggggaagttc
     2221 ctggcggtcc tcaccgcctc ggtgggcgcg gtcgccatcg tggtcacgac catgatgctg
     2281 agcctccact ggggctaccc gtacctgttg cacacgtcga aaaaactgga tgtcaccctg
     2341 ccgctcggga ccgccgtggt gctgttcggc gtggccctgt tgttctccgc gctggtcagc
     2401 gcgatccagc tcgccgtcag catctacgcg cggtcccccc gggaagcgca gcagtacgtg
     2461 accccgctgt acttcgccat cgtcctgccg gccctggcag tgcagttcat cagcgaatgg
     2521 caggggacgt tgtgggtgta cctggtgccg gtgctgaaca cgttctttgc cttccgcgag
     2581 ctgttgctgg ggacggtcaa ctgggcgcac ctggcgctcg tcgcggtgtc ctgcgtgttc
     2641 tacgctaccg cggtgctgga tctggccgtc gcgtttttca gccgggagag cgtgatcttc
     2701 cgcacctagg cgcaccacgg gggcttgcgg tcgtcagaat cggcgaggga accccagcga
     2761 ccgcaaaagc gggggcgcca gccccgccca tcagtcccgc ggggagcgtt tccaccttgt
     2821 ggacactaca tcttgtgtgc tactatccct gttgcgccaa gatataggag gagatacgaa
     2881 cacctgtgcg atgcccttat tgcagcggcg aagatagccg ggtcctcgat tccaggggga
     2941 tcgacgaggg tgaggcgatc cgccgccggc gggagtgcct cgagtgccgg cgccgcttta
     3001 cgacgttcga gcgggcggag cgcctggtgc tccacgtcat caagcgggat gggcgccgcg
     3061 aggcgttcga ccgggcgaag gtgttgaccg gcatgctccg ggcgtgcgag ggccggccgg
     3121 tcccccgcga ggtcctcgag cagacggcgg gagagatcga gcgcgagctc ctcgaggggg
     3181 gggcggcgga tgtgtcaagc cgcgagatcg gagaccgggt gatcgagcgg ctccgccgga
     3241 tggacgatgt ggcgtacgtc cggttcgcct ccgtgtaccg cagggttggc gacgtggatc
     3301 ggctggtcga ggagatccag cggctcaagg cgcgcaagca actcgaagcc gagttgcagg
     3361 cgcagatcga actgattccg ctcttgcccc agaatcccgg acagaactaa tcaggcccgt
     3421 gcccccgtct cgcacatctt atttccttct ggaggaggcc gatggcgata acatctgaac
     3481 cgaccgccgc cccgacggag gcgccgtcgg cccccatgtc ggtgtcgaag gagaccctcc
     3541 agttcttttc tggagacgag ctgcgcgccc gcgtcttcag cgacaagtac gccctgcggg
     3601 acctcgacgg gcagatcctg gagcggacgc ccgtcgatat gtggcggcgc gtcgcggacg
     3661 gggtcgcgag cgtcgagccg acggaagagg cgcgggaacg gtacgcgcgg atgttctact
     3721 ggctcctgcg ggacttccgg ttcatccccg gcggccggat catgcacgcg gtgggcaacc
     3781 ccaagcgcgt cactgccctt aactgcttcc cggcggggac ccgtgtgctg acgcgagaag
     3841 ggttcaaggt catcgaagag atccgagctg gcgatgaggt cctgacgcac cggaaccgct
     3901 tccgcagtgt cacccacacc atgcaccgag aggctgcaga gccgctgcga acagtgaggc
     3961 tctggtacct tggcgatcgg cagattgcgg ctaccaaaga gcatcgcttc ctggcttacg
     4021 acggctcacg cgtggactgg gtggaagcgc agcacctcac cccgtctcac tatgtcaagg
     4081 ccgggcggat cgacgagacg ctgccggtgc gggagcttga cctgtccgag tacgtcagcg
     4141 cggccgctgt cgaggacgaa gtcggaaaac tctatacggt cacatccttc gttggtggtc
     4201 agggcgccag gcgtcgcata gagagtaagc gagtctcccg cactgtcccg gtcgacgagc
     4261 ggttcggtct gtggcttggg ttcttcatgg ccgagggcgg catcaccgac aacagcgtgt
     4321 actttacgtt caataagaat gaggaggctt acgctgagag catctacaat ctgacgcagc
     4381 aattgttcgg cgtagaggcc gcgattcagc gtcgcgaggg ccaggagggg cagtggctgc
     4441 gcgtgtatgt gcacagcaag atgatggtcg ag
//
LOCUS       DASQGH010000304        11127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52189, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000304 DASQGH010000000
VERSION     DASQGH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52189"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VEW91_05985"
     CDS             <1..832
                     /locus_tag="VEW91_05985"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYM91165.1"
                     /translation="TQDIGTGTRTALTQIAAEVLSLPVDRVTLSLGDTDFPYSPLSAG
                     SQTIASCGPAVRMAAEEARRHLIEAAASALEAAATDVRLASGQHHVAGVPDRRISVAD
                     LTRRMGNFTIAGRGFRGANPDNVTIRTWGAQFAEVEVDTETGDVAVLKISACHDVGRI
                     INPLQYTSQVHGGVIMGLGLALSEDHVTDPETGNVLDLGFDAYAVPRQSHVPEIDALA
                     VGIPDPVANNLGVKGVGEPPIIPTPAAIANAVANAIGVRITDLPITPMKVLRALGRVE
                     "
     gene            complement(833..2779)
                     /locus_tag="VEW91_05990"
     CDS             complement(833..2779)
                     /locus_tag="VEW91_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYM91166.1"
                     /translation="MAGPITALRTRPALGQVIDWLVLLGVLGLIAGMAFGGVTFLPAA
                     GFAQNRPPLLAFDQAALLIGAGAFGRWLLLGGRALPRALLWGVSAVAALGLLSLVTTT
                     DLSATREAVLFLVAVVVTALAVAICATDGGTARAMLAALALLGVGEALIGLGQYASGA
                     PTPAYWLSRAFAGVIRTRVHGTLGNPNVLANFLLVGIGATVLLAVDLGGRRRVLPMVA
                     LAVEIAALALTYSRGGYLGLAAFVLAAALLLWPVRRRAWPVLLVVAVMAGLAALALPA
                     VGLRGESIGLDPGDTAQSRFFIWRTAGRMWQAHRVWGTGIGTFNAAYPSYRPLGVRTT
                     YAMLRIPGSAHNDYLQVLAETGVAGAGLVALALAWGVGRATRRYRKGGPADRVWIGTW
                     GATCAAIGVTSLVDSTLSVIPTTAMVAAFTAAVAAHESLDAPPLRSPKRLLMLPIVAL
                     LAGLPPLLAPLAQASALDAQAHRLVRAGRYVDAVDAFRRAAAADPLSGEALPYFGDLL
                     ADLYLRKIDSSVGEWRTARARAAELYARAGRLSPWDGYPRAALGRLRRAEGRYPEARA
                     ALLAAIALDPYSPRYRLWLGETLAAAGDPRGAVEPLREAARLYPIELLVIERHEGRSA
                     AYAQDQADLGEARRLLTRLGEAAP"
     gene            complement(2764..3528)
                     /locus_tag="VEW91_05995"
     CDS             complement(2764..3528)
                     /locus_tag="VEW91_05995"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016736740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYM91167.1"
                     /translation="MSETSPMFSLDGQVAVVTGASRGIGRGIATALAGAGADIVAVSR
                     HPAETLAAEVEALGRRCHVVTADLGDPGQVETVLPRAVATAGRVDILVNNAGIATRGP
                     ALDVPLEAWHRVLQVNLHAVFRLAQAAAREMLPRRRGKIINVASLMSFEGGILVSPYT
                     ASKGAVGQLTKALANEWAPHGINVNGIAPGYIATDLTRPLQEDTTRNPAILARIPAGR
                     WGRPEDLGGAAVFLAARASDYVHGHLLVVDGGWLAR"
     gene            3609..4892
                     /locus_tag="VEW91_06000"
     CDS             3609..4892
                     /locus_tag="VEW91_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYM91168.1"
                     /translation="MRRTGQAVLHTRRTTRRSLVAVVVVLLLTSGVAAAAPKARLEFW
                     TISLQPFFTGYIQDLIGRYERDHPGLAIRWIDIPAQVIDQKLLAAIAGGVSPDVVNLN
                     TEGTLRMAQARALAEMDAGVPPSARARYFPNIWDSLRYQGRAYGIPWYVVPNVLAYNR
                     ALFRRAGLDPAHPPASEEAFIQTATTIKARTGVYGFMPNVDGIRFLRVFQEDGLPILS
                     PDGRRAVFNSPEHARVLARYVQLFTRDLFPSDTLERGYLGATERYAAGQLAMLTTGPQ
                     FLLRVRTDSPEVYRETAVAPYPLGRGRVLDLPTMALTVPLASRHRAEAVEFALFATND
                     ANQLAFCRLVVIFPSTRAAAADPYFTRGGPSPEERARQIAATELSSARDLTVVVSHSD
                     DLYRVFREAVERAFFGRMTPQQALDWAVKEWSSKL"
     gene            4874..5782
                     /locus_tag="VEW91_06005"
     CDS             4874..5782
                     /locus_tag="VEW91_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM91169.1"
                     /translation="MEQQAVARSPSTPAVRRALTAYLFLLPALVLLGAFTFYPVAFGT
                     ALSLFEYNVIAPPRFTGAANFRALWSDRYFWIALENSLKYLAVVPVLQGCALLLAVWV
                     HRSARGLGWVRAAYYLPVVTSIVVVGILWRWLYDDAGLINYVLLQLRLLPAPVHWLAS
                     PGIALYAVMFVTLWKGLGYYMVIYLAGLATIPPGYAEAAAIDGAGSLQQVWRVTLPLL
                     RPSILLATTLSAISALRVFEEVYVMTSGGPMYATYTMFYYMFDQAFGALHLGYAAAIG
                     VVLAAVTIGLSIANFKWLREGGLSYY"
     gene            5826..6656
                     /locus_tag="VEW91_06010"
     CDS             5826..6656
                     /locus_tag="VEW91_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM91170.1"
                     /translation="MRRTVRRRLSAVVECAGLVLLAVFTAFPFVWLLATSLRGSGTVF
                     AFPPVIIPRPPTLANFVGVWNTMPLGRFFLNTAYITGMGIALTLVTSALAGYPLARMR
                     FPGRDAIFYAIVGSLMLPQHVGLILNFVTLMRLHLIDTYAAVYLPSAASVLGIFLLRQ
                     AYLTIPTEIEDAARIDGAGELRLWGQIMLPLVAPALATLAIIEFSAYWNSFLWPLIVL
                     KSPERYPLAVGLLYLSGLFAHNTRYVAAGAVVMTLPMIVVFLALQRYFLRGITIGAVK
                     "
     gene            6664..8142
                     /locus_tag="VEW91_06015"
     CDS             6664..8142
                     /locus_tag="VEW91_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HYM91171.1"
                     /translation="MRIAFLPLDDRPVTRGAFLRLGEIAGVEVATPPRALLGARRRPA
                     DVEALWVWIDADGADADLLIASAELLIYGGLVPSRIGDEPLDRCLALAGRFARLRHRS
                     PRRRVWLAASNLRLPSTPDASEEPEYWAEHGPQIFAYSYHADRFAQTGDPASQRLAAA
                     AEGAVPKPVLADVMARRARNLAVLLSLVDQAAGGEIDGLLIGQDDAAEFGLTRRDLRA
                     VETAIVDRGAGARAWITYGTDELGVRLLARALLAARGQAPGVGAVYAYPEHRDAIPRY
                     EGQRLDQTVTSHIATSGCRRADGGGDLTLFLHNFPGVQEEAPAQEPYDPELLAPFLRA
                     VSAAAARGQRFGIADIRYSNGADRTFVQRLLQAPRAYGTLAYGGWNTSSNTIGMVLAQ
                     MLLPHGPAAEAFTMTRFLDDWAYQAGVRQRLASEIVPRYPGAASQRLGPALGPCAEAA
                     RVWLQRDYVPPLERCFGRRITITKLAFPWERLFEVDLEIVVQ"
     gene            8139..9122
                     /locus_tag="VEW91_06020"
     CDS             8139..9122
                     /locus_tag="VEW91_06020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYM91172.1"
                     /translation="MTADQAIAAFHPELLPEVLGVWNAALGDRFPLSRDLFMQNAILD
                     PHFDPQGFWVARAPNEETVIGICLAKIAREPLAADGYLPDRGWISLLAVHPASQRRGV
                     GRALLRQAERYLRAHRRRRAVLGGDPDHFVPGIPRAPGALAFFAACGYELAGDAYDLR
                     RPIAGYETPASVAATMAAHPDLEIRPLGTDEASRLLAFLDEVFPGRWRYTVGRFLDLG
                     GSIADIMGIVQGGRVCGFAHLFHPASRWIGPSIAWTHGAARRAGGIGPMGLAPALRGR
                     GLGLALLDRAVRRLADLGIEDVTVDWTNLVAFYGRLGFAPWRQYRHGEKPL"
     gene            9137..10693
                     /gene="nagZ"
                     /locus_tag="VEW91_06025"
     CDS             9137..10693
                     /gene="nagZ"
                     /locus_tag="VEW91_06025"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HYM91173.1"
                     /translation="MGARGGDPETLDRQGLVGQMFMVDFSGPEPSAEVERLLTEGGVG
                     GVVVFDKNVEAPIQVARLSNALQRLAARAGQPPLLLAADQEGGPVVRLRGTHFPSAMA
                     FGAAASDRLVAQAARVTARELRAVGIHVNFAPVLDVNSNPANPVIGVRSYGEDPAPVA
                     RLGVQAVTAMQAAGVLATAKHFPGHGDTSLDSHLALPTVAHPRGRLEAVELLPFVAAI
                     RAGVAAVLTAHVVYPALDPDRPATLSPAIIGVLRRELGFQGLVVSDSMRMRAIVDHYR
                     PGDAAVEAVLAGVDLVLALGPAEAQWEAIEAVRAAAETGRIPSARLHEAAGRVLAAKR
                     RLGLFERALVREGDVAGSVGLPEHIALAHRIAAAAATNVRDRPGAVPLPPGSVSVATG
                     LAPGDTVARLAEALRAAGRPAASVTLGPETPPQGAIVVPIGGGAPSDPDFSARVYEVT
                     LEASGYGPTIAVSTDVPYPLARVAPRCACLAVYGADPAALHAAAGVLTGAVRPRGRLP
                     VTVSAAPDAA"
     gene            10847..>11127
                     /locus_tag="VEW91_06030"
     CDS             10847..>11127
                     /locus_tag="VEW91_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91174.1"
                     /translation="MARAPDEPLHRRLLALLDDAVKEDEVTASGLATQLAAPVSEVEA
                     ELRDLVRFGNLTRRPGLNGEARYSVGPRRKLLGEMLVEAGHMTKEQLEEA"
BASE COUNT         1477 a         4035 c         3969 g         1646 t
ORIGIN      
        1 gacgcaggat atcgggacgg gcaccaggac cgcgctcacg cagatcgctg ccgaggtgct
       61 gagccttccg gtggaccgcg tcaccctcag cctgggcgac accgacttcc cctacagccc
      121 gctctccgcg ggaagccaga cgatcgcgtc gtgcggcccc gccgttcgca tggcggccga
      181 ggaggcgcgc cggcacctca tcgaggccgc cgcatcggcg ctcgaggccg ccgccaccga
      241 tgtcaggctc gcctctgggc agcaccacgt cgccggcgtc ccggaccgga ggatctcggt
      301 ggccgacctc acccggcgga tgggcaactt cacgatcgcc ggccgcgggt tccggggcgc
      361 caacccggac aatgtgacga tccggacatg gggggcgcag tttgccgagg tcgaggtcga
      421 caccgagacc ggcgacgtgg ccgtcctgaa gatttccgcc tgccacgatg tcggccggat
      481 catcaacccg ctgcagtaca cgagccaggt ccacgggggg gtgatcatgg ggctggggct
      541 cgccctgagc gaggatcacg tcaccgaccc ggagaccggc aacgtgctcg atttgggatt
      601 tgacgcctac gcggtccccc gccagtcaca cgtgcccgag atcgatgcgc tcgccgtcgg
      661 catcccggat cctgtcgcca acaacctcgg cgtgaagggc gtgggcgagc cgccgatcat
      721 cccgaccccc gccgcgatcg ccaacgcggt cgcgaacgcg atcggggtcc gcatcaccga
      781 cctcccgatc acgcccatga aggtcctgcg ggcgctcggt cgggtggagt agctacggcg
      841 cggcttcccc gagccgcgtg aggaggcggc gggcttcccc gaggtcggcc tggtcctgcg
      901 cgtacgcggc gctgcgccct tcgtggcgct cgatgacgag caactcgatc gggtagagcc
      961 gggcggcctc gcgaagtggc tccaccgccc cgcgggggtc ccccgcggcc gccagcgtct
     1021 ccccgagcca gagccggtac cggggcgagt acgggtcgag ggcgatggcc gcgagcaacg
     1081 cggcccgggc ctcggggtag cgcccctcgg cccggcggag ccggcccagg gcggcgcgcg
     1141 ggtagccgtc ccacgggctc aggcgtccgg cccgggcgta gagctccgcc gcgcgcgctc
     1201 gggccgtccg ccactccccc acgctgctgt cgatcttccg taggtagagg tcggccagga
     1261 gatccccgaa gtacggcagc gcctcaccgc tgaggggatc ggcggccgcc gccctgcgga
     1321 atgcgtccac ggcgtcgaca taccgtccgg ctctgaccag gcggtgggcc tgcgcgtcga
     1381 gcgcggaggc ctgggccagg ggggcgagca gcggaggcag tcccgccagg agggcgacaa
     1441 tcggcagcat cagcagccgc ttcggggacc gcagtggcgg ggcatccaga gactcgtggg
     1501 cggcgacggc ggcggtgaac gccgcgacca tggccgtggt cggaatcacc gacagggtcg
     1561 aatccaccag gctcgtgacg ccgatggcgg cacacgtcgc cccccaggtc ccgatccaga
     1621 cccggtcggc gggcccgccc tttcggtacc ggcgggtggc ccgccccact ccccacgcga
     1681 gcgcaagcgc caccagcccc gcgccggcca cccccgtctc ggcgagcacc tggaggtagt
     1741 cgttgtgcgc cgaccccggg atcctcagca tcgcgtaggt cgtgcggacc cccaagggcc
     1801 tgtacgatgg atacgcggca ttgaacgtgc cgatcccggt cccccacacg cgatgcgcct
     1861 gccacatgcg cccagccgtc cgccagatga agaaccggct ttgggcggtg tcccccggat
     1921 caagcccgat gctctcgccc ctgaggccga ccgccggcag cgcgagcgcg gcgagcccgg
     1981 ccatcacggc gaccacgagc aggacgggcc aggcgcgccg gcggaccggc cagaggagga
     2041 gggcggcggc gagcacgaac gcggcgaggc caaggtaccc gccgcgggag tacgtgagcg
     2101 cgagggcggc gatctccacg gccagcgcca ccatgggcag gacccgcctg cgccctccga
     2161 ggtccaccgc gagcagcacc gtcgcgccaa tcccgaccag caggaaattc gccaggacgt
     2221 ttgggttgcc gagggtgccg tggacgcggg tccggatcac cccggcaaag gcgcggctga
     2281 gccagtacgc cggcgtgggc gcgccgctcg cgtattgccc caacccgatg agcgcctcac
     2341 cgacgcccaa gagcgccagg gcggcgagca tggcccgcgc cgttccgccg tccgtagcgc
     2401 agatcgccac cgccagcgcc gtcaccacga cggcgacgag gaagagcacc gcctccctgg
     2461 tcgcggacag gtcggtcgtc gtgaccagcg agaggaggcc gagggctgcg accgcggaca
     2521 cgccccagag cagcgcccgc gggagcgccc ggccaccgag cagcagccag cgcccgaacg
     2581 ccccggcccc gatcaggagc gccgcttggt cgaacgccaa gagcggcggc cggttctgcg
     2641 caaacccggc cgccggcagg aacgtcaccc ctccaaacgc catcccggcg atgagcccca
     2701 gcacgcccag gagcaccagc cagtcgatca cctggccgag cgcgggccgg gtccgaagtg
     2761 ccgttatcgg gccagccatc ccccgtcgac gaccagcagg tgcccgtgga cgtagtcgga
     2821 ggcgcgcgcg gcgagaaaca cggcggcgcc cccgaggtcc tccggccgcc cccagcggcc
     2881 ggcagggatg cgggcgagga tcgcggggtt ccgggtggtg tcctcctgca gcgggcgggt
     2941 caggtcggtc gcgatgtagc ccggcgcgat cccgttgacg ttgatgccgt ggggggccca
     3001 ttcgttggcg agggctttgg tgagctgccc caccgcgccc ttgctggcgg tgtacgggct
     3061 caccaggatc ccgccttcga agctcatcag cgacgccacg ttgatgatct tcccgcgccg
     3121 gcgcgggagc atctcccggg cggcggcctg ggccagccgg aacacggcgt ggaggttgac
     3181 ctgcagcacg cggtgccagg cctcgagggg cacatcgagc gcgggcccgc gcgtggcgat
     3241 gccggcgttg ttcacgagga tgtccacccg ccctgccgta gcgaccgcgc ggggcagcac
     3301 cgtctcgacc tgcccgggat ccccgaggtc ggcggtcacc acgtggcacc gcctcccgag
     3361 ggcctcgacc tcggcggcga gcgtctccgc cgggtggcgg ctcaccgcga cgatgtcggc
     3421 ccccgccccg gccagtgccg tggcgatccc gcggccgatc ccgcggctcg ctccggtgac
     3481 caccgcgacc tgtccgtcga ggctgaacat cgggctggtc tcgctcatcg atcccccacc
     3541 ctcctcgcgc accaacgccc actctgttcg cagcggggcg ccccggctcc cgcaggaagc
     3601 ggctgcgggt gcggcgaacc gggcaggcag tgttgcacac gcgccgcacc acgagacgca
     3661 gcctcgtcgc cgttgtggtc gtcctcctgc tcacgtccgg tgtcgcagcg gcggccccca
     3721 aggcgcggct ggagttctgg acgatctccc tgcagccgtt cttcaccggc tacatccagg
     3781 acctgatcgg ccgctacgag cgcgatcatc ccgggctggc cattcggtgg atcgacatcc
     3841 cggcgcaggt gatcgatcag aagctgctgg cggcgatcgc cgggggcgtg agccccgatg
     3901 tggtgaacct caacaccgag gggacgctgc ggatggcgca ggcgcgcgcc ctggccgaga
     3961 tggacgcggg ggtgccgccc tcggcgcgcg cccgctactt tccgaacatt tgggactcgc
     4021 tgcggtacca ggggcgcgcc tacgggatcc cgtggtatgt ggtgcccaac gtgctcgcct
     4081 acaatcgcgc gctgttccgc cgcgccggat tggatcccgc ccatccgccg gcatccgaag
     4141 aggcgttcat ccagacggcc acgaccatca aggcccggac gggcgtctac gggttcatgc
     4201 ccaacgtgga cgggatccgg ttcctccggg tgttccagga ggacggcctc ccgatcctca
     4261 gcccggacgg ccgccgggcc gtgttcaaca gcccggagca cgcgcgggtg ctggcgcgat
     4321 acgtccagct gttcacgcgg gacctgtttc cgtcggacac gctggagcgc gggtacctgg
     4381 gcgcgaccga gcgatacgcg gcgggccagc tggccatgct caccaccgga ccccagtttc
     4441 tcctccgcgt tcggacggac agccccgagg tctaccggga gaccgcggtg gctccctacc
     4501 ccctcggccg ggggcgggtc ctcgacctcc ccacgatggc gctgaccgtg cccctggcga
     4561 gccgccaccg cgcggaggcg gtggagttcg cgctgttcgc cacgaacgac gccaaccagc
     4621 tggcgttctg ccgcctggtg gtgatcttcc catccacgcg ggccgcggcg gcggacccgt
     4681 acttcacccg cggcggcccc agccccgagg agcgcgcccg gcagatcgct gcgaccgaac
     4741 tgtcgagcgc ccgcgacctc accgtggtcg tctcgcacag cgacgacctc taccgggtgt
     4801 ttcgggaggc ggtcgagcgc gcgttcttcg gccggatgac cccacagcag gccctggact
     4861 gggcggtcaa ggagtggagc agcaagctgt agcgcggtcc ccgtcgaccc cggccgtccg
     4921 ccgcgccctg accgcgtacc tgttcctgct gccggcgctg gtgctgctgg gcgcgttcac
     4981 cttctatccg gtggcgttcg gcaccgccct gagcctgttc gagtacaatg tgatcgcgcc
     5041 cccccggttt acgggcgccg caaacttccg ggcgctgtgg agcgaccgct atttctggat
     5101 cgcgctcgag aacagcctga agtatctggc cgtcgtcccc gtcctccagg ggtgcgcgct
     5161 cctgctcgcc gtctgggtcc accgctcggc gcggggactg ggatgggtga gggccgcgta
     5221 ctaccttccg gtcgtcacct cgatcgtggt cgtcgggatc ctgtggcggt ggctgtacga
     5281 tgacgccggc ctcatcaact atgtcctcct gcagctgcgg ctcctccccg ctcccgtcca
     5341 ctggctcgcg agcccgggga tcgccctcta cgccgtcatg tttgtcaccc tctggaaggg
     5401 cctcgggtat tacatggtga tctacctcgc ggggctggcg accatcccgc ccgggtacgc
     5461 ggaggccgcg gcgatcgacg gggcaggatc gctgcagcag gtgtggcggg tgacactgcc
     5521 attgctccgg ccgagcatct tgctggcgac cacgttgtcg gccatctccg cgctgcgcgt
     5581 gttcgaggag gtgtacgtga tgaccagcgg cgggccgatg tacgcgacgt acacgatgtt
     5641 ctactacatg ttcgaccagg ccttcggcgc cctccacctc gggtacgcgg ccgccatcgg
     5701 ggtggtcctg gccgccgtga ccatcggtct ctcgatcgcc aatttcaagt ggctccggga
     5761 aggggggctc tcgtattact gaggcgatcg gatcccggcc ccccgccccc accgaccgcg
     5821 gtcgggtgcg ccgcaccgtc cgccggcgcc tctcggcggt cgtcgagtgc gcggggctgg
     5881 tcctcctggc ggtcttcacg gcttttccct ttgtctggtt gctcgcgacc tcgctccggg
     5941 ggagcggcac cgtcttcgcg tttccaccgg tgatcattcc gcgcccgccg acgctggcca
     6001 acttcgtggg ggtctggaac acgatgccgc tcggccggtt cttcctcaac accgcgtaca
     6061 tcaccgggat ggggatcgcg ctgaccctgg tgaccagcgc gctggccggg tatccgctcg
     6121 cgcggatgcg ctttcccggg cgggacgcga tcttttacgc gatcgtgggg agcctgatgc
     6181 tgccccagca cgtggggctg atcctcaact ttgtgacgct gatgcggctt cacctgatcg
     6241 atacctatgc cgccgtctac ctgccgagcg cggcgagcgt gctcggcatc ttcctcctgc
     6301 ggcaggcgta cctgacgatc cccaccgaga tcgaggatgc ggcccggatc gacggcgcgg
     6361 gcgagttgcg gctgtggggc cagatcatgc tcccgctcgt cgccccggcc ctcgccaccc
     6421 tggcgatcat cgagttctcc gcgtactgga actcgtttct gtggcccctc atcgtgctca
     6481 agtcgcccga gcggtacccg ctcgccgtgg gcctcctcta cctctccggc ttgttcgcgc
     6541 acaatacccg gtacgtcgcg gcgggggcgg tcgtgatgac cctgccgatg atcgtggtgt
     6601 tcctggcgct ccagcggtac ttcctccggg ggatcaccat cggcgcggtc aaatgagcgc
     6661 cccatgagga tcgcgttcct gccgctcgac gaccggcccg tgacgcgggg ggcgtttctc
     6721 cgcctcgggg agatcgccgg ggtcgaggtg gcgacccccc cgcgcgcgct cctgggcgcc
     6781 cgccgccgtc cggccgatgt cgaggcattg tgggtctgga tcgacgccga cggcgcggac
     6841 gccgatctcc tgatcgcctc cgccgagctg ctgatctacg gcgggctggt cccctcacgg
     6901 atcggcgacg agccgctgga tcggtgcctg gcgctggccg ggaggttcgc gcgcctgcgg
     6961 caccggtcgc cccgccggcg ggtgtggctt gcggcgagca acctgcggct gccgtccacg
     7021 cccgatgcca gcgaggagcc ggagtactgg gccgagcacg gcccccagat ctttgcgtac
     7081 agctaccacg cggaccggtt cgcgcaaacc ggagacccgg cgtcgcagcg gctcgccgcc
     7141 gccgccgaag gggcggtgcc caagccggtc ctcgccgatg tgatggcccg gcgggcgcga
     7201 aacctggccg tgctgctctc cctggtcgac caggccgcag gcggtgagat cgatggtctc
     7261 ctgatcggcc aggacgacgc ggcggagttt ggactcaccc ggcgcgatct tcgggcggtc
     7321 gagacggcga ttgtggaccg gggtgccggc gcgagggcgt ggattaccta cggcaccgac
     7381 gagttgggcg tccggctgct ggcgcgggcg ctgctggcgg cgcgcgggca ggcgcccggc
     7441 gtcggggcgg tgtacgcgta tccggagcat cgggacgcga tcccccggta cgaggggcag
     7501 cgcctcgacc agacggtgac ctcgcacatc gcgacatccg ggtgtcgccg cgcagacggc
     7561 gggggagacc tgacgctctt cctccacaac ttccccggcg tccaggagga ggcgcccgcc
     7621 caggagccgt acgacccgga gctcctcgcc ccgtttctcc gcgccgtgtc cgccgcggcg
     7681 gcgcgcgggc agcggttcgg gatcgcggac atccgctaca gcaacggagc cgaccggaca
     7741 ttcgtgcagc gcctcctcca ggccccccgg gcatacggca cgctggcgta cggcgggtgg
     7801 aacacctcga gcaacacgat cgggatggtg ctggcgcaga tgctcctgcc ccacgggccg
     7861 gcggccgagg cgttcacgat gacccggttc ctcgacgact gggcgtacca ggccggggtg
     7921 cggcagcggc tcgcgtccga gatcgtgccg cggtatcccg gtgcggcctc ccagcgcctc
     7981 ggtccggccc tcggcccgtg cgccgaagcg gcacgggtgt ggctccagcg cgactacgtc
     8041 cccccgctcg agcggtgctt cgggaggcgg atcaccatca cgaaactcgc gtttccctgg
     8101 gagcgcctgt tcgaggtgga cctcgagatc gtcgtgcaat gaccgccgat caggccatcg
     8161 ccgccttcca cccggagctg ctgcccgagg tgctcggcgt gtggaacgcc gcgctgggcg
     8221 accggttccc gctctcccgg gacctgttca tgcagaacgc gatcctggat ccgcacttcg
     8281 accctcaggg gttctgggtg gcgcgggctc cgaacgagga aaccgtgatc gggatctgcc
     8341 tcgcaaagat cgcgcgggaa ccgctggccg cggacgggta cctcccggac cggggctgga
     8401 tcagcctgtt ggcggtccat ccggcctccc agcgccgcgg ggtcgggcgg gcgctgctcc
     8461 ggcaggcgga acgctacctc cgcgcgcatc ggcgccgccg tgccgtgctc ggcggggacc
     8521 ccgaccactt cgtcccgggg atcccccgcg cacccggcgc gctggcgttc tttgccgcgt
     8581 gtgggtacga gctggccggg gatgcgtacg acctccggcg gccgatcgcg gggtacgaga
     8641 cccctgcctc cgttgcggca acgatggcgg cccacccgga tctggagatc cgcccgctcg
     8701 ggacggacga ggcctcccgg ttgctcgcgt ttctcgacga agtgtttccc gggcggtggc
     8761 ggtacaccgt ggggcggttc ctcgacctgg gcggatcgat cgcggacatc atggggatcg
     8821 tgcaaggcgg ccgggtctgc gggttcgcgc acctcttcca ccccgcgtcc aggtggatcg
     8881 gcccgagcat cgcctggacc cacggggctg cccggcgcgc gggcgggatc ggcccgatgg
     8941 gcctggcccc cgcgctccgc gggcgcgggt tggggctggc gctcttggac cgggcggtcc
     9001 gccggctggc ggaccttggt atagaagacg tgacggtgga ctggacgaat ctcgtcgcgt
     9061 tctacggacg gctggggttt gccccctggc ggcagtaccg ccacggggag aagcccctct
     9121 gacgaacggg cggaccatgg gcgcgcgcgg gggagacccg gagacactcg accggcaagg
     9181 gctcgtcggc cagatgttca tggtcgattt ctccgggccc gagccatccg ccgaggtcga
     9241 gcgactcctc accgagggcg gcgtgggcgg cgtcgtcgtc ttcgacaaga acgttgaggc
     9301 cccgatccag gtggcccggc tctcgaacgc gctccagcgc ctcgccgccc gcgccgggca
     9361 gcccccgctg ctgctggcgg ccgaccagga gggcggcccg gtcgtgcgcc tgcggggcac
     9421 gcacttcccg agcgcgatgg cgttcggcgc cgcggccagc gatcggcttg tcgcccaggc
     9481 cgcccgggtg accgcgcgcg aactccgcgc cgtgggcatc cacgtcaact tcgccccggt
     9541 gctcgatgtg aacagcaacc cggcgaaccc cgtgatcggg gtgcgctcgt acggcgagga
     9601 tccggcgccg gtggcgcgcc tgggcgtcca ggcggtaacg gcgatgcagg cggccggcgt
     9661 gctggccacc gccaagcact ttccagggca cggggacacc tcgctcgatt cccacctcgc
     9721 gctccccacc gtggcgcacc cgcgcgggcg gctcgaggcc gtcgaactcc tcccgtttgt
     9781 ggcggcgatc cgcgccggcg tggccgccgt cctgaccgcc cacgtggtct acccggcgct
     9841 cgatcccgat cgccccgcca cgctctcgcc ggcgatcatc ggcgtattgc gccgggagct
     9901 cggatttcag gggctcgtgg tcagcgattc gatgcggatg cgggcgatcg tcgaccatta
     9961 ccgtcccggg gacgccgcgg tggaggccgt gctggcaggg gtcgatctcg tactggcgtt
    10021 gggcccggcg gaggcgcagt gggaagcgat cgaggcggtg cgggccgcgg cggaaaccgg
    10081 ccgcattcca tccgcccggc ttcacgaagc cgccgggcgc gtcctggccg ccaagcgccg
    10141 gcttgggctt tttgagcgcg cgctcgtgcg tgagggggac gtggcaggat cggtgggcct
    10201 gcccgaacac atcgccctcg cccaccgtat cgccgcggcg gctgccacga acgtccggga
    10261 ccggccgggg gccgtcccac tcccgcctgg gtcggtctcg gtcgcgaccg gtctggcgcc
    10321 tggggacacc gtggcgcggc tggccgaggc gctgcgcgcc gccggccggc cggccgcgtc
    10381 cgtgacgctc ggccccgaaa cgccccccca aggggcgatc gtggtcccca tcggcggggg
    10441 agcgcccagc gatcctgact tttccgcgcg agtctacgag gtgacgctag aggcatccgg
    10501 gtacgggccg accatagcgg tgtccacgga cgttccctac ccgctggccc gcgtggcccc
    10561 gcgctgcgcc tgcctggccg tgtacggggc cgacccggcg gccctccatg cggcggcggg
    10621 cgtgctcacc ggagccgttc gcccgcgggg ccgcctgccc gttacggtgt cggctgcacc
    10681 cgacgccgcg tagggcggct gagaaggtct gagacccccc ccaaaaagtg catatttcat
    10741 aaggataatc tacgtttccg cagaggctgg cccacttggg ctccggcgct gcggggatgg
    10801 gcttttggag gggccatgag ggtcatgtgg atggcggagc gtaaacatgg ctcgcgcgcc
    10861 tgacgagccg cttcatcgcc gcctgctcgc gctccttgac gacgccgtca aggaggacga
    10921 ggtgacggcc tccggcttgg ccacgcaact ggccgcgcct gtgtccgagg tcgaggccga
    10981 gctacgggat ctggtccgct ttggcaatct cacccgccgg cccggcctga acggcgaggc
    11041 ccggtacagt gtcggcccca ggcggaaatt gctcggcgag atgctggtcg aggccgggca
    11101 catgaccaag gagcagctcg aggaggc
//
LOCUS       DASQGH010000305         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35320, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000305 DASQGH010000000
VERSION     DASQGH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35320"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1309)
                     /locus_tag="VEW91_06035"
     CDS             complement(89..1309)
                     /locus_tag="VEW91_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM91175.1"
                     /translation="MRSSFAFDAPAYRWLWLYSLFSAMAFTVDLLSQGWVVLQLTNSA
                     FWVGLAAGLRGVSQGLFSVLAGPVVDRLDRRRLVVAGQLAAALGALTLAVLLLNHAAR
                     LWHILCYLALIGLVNAVTKPSASSLMYDVVGPERLLNASALQFMAGSLVRIVGALAAG
                     VVIDRLGVGKNYVLVCAAYCGGAAALLFLRNPAAAPRAAQPFVRAVMAGLGYALRVRE
                     IRTLLMLSLVVEAFGFGYQAMMPVMARDVLKVGGTGLGYLTAMAGAGQLVATLLVASR
                     GDLRNKGALLAAAAAGFGLCIVAFALSPWFAFSLVVVMGVGGLGSAYDTSMSTVLITT
                     AGDDVRGRVLSLYYATMGVSAFGWLGIGAVAAFLGAPVALALSGSIVALGALGFLSRL
                     SVFGRPGTAVAGPE"
     gene            1474..1839
                     /locus_tag="VEW91_06040"
     CDS             1474..1839
                     /locus_tag="VEW91_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM91176.1"
                     /translation="MVKDLGWLNSYQGLIIRVAADAFGVLLMRQFMLHLPDSLIEAAR
                     IDGASELGTYFRIVVPLIWPAVLTLTIFAWRETWDAFVWPFIIITQDSLRTVSIGLQR
                     FPEEYVTPTTRSWRSRPSR"
     gene            complement(2379..>2596)
                     /locus_tag="VEW91_06045"
     CDS             complement(2379..>2596)
                     /locus_tag="VEW91_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91177.1"
                     /translation="VYYATQDGLLHIGMLEDSVRGILGRPLRTFQWSHFKVLYYHGMS
                     FTVDMDNMHIVTGIAVIPNGVSAQAPR"
BASE COUNT          510 a          879 c          784 g          423 t
ORIGIN      
        1 acaggatgat ggaggagatg cgcgggaagt ttctacgggc ccaacttccc tacaatttgt
       61 ctggaatcac cccccgaagt ttcccgcctt actcggggcc ggcgacggcc gtgccaggtc
      121 ggccgaaaac cgaaagcctg gacaagaacc caagcgcacc gagcgcgacg atgctcccgc
      181 tgagtgccag ggccacgggc gcacccaaga acgcggcgac ggccccaatc cccagccatc
      241 cgaaggcgct gacacccatc gtggcgtagt agaggctcag cacgcgcccc cgaacgtcgt
      301 cgcccgctgt ggtgatcagc accgtcgaca tggacgtatc gtacgctgat cccaaccctc
      361 ccacacccat gacgaccacg agcgagaacg caaaccaggg tgagagggca aaggcgacga
      421 tgcacaggcc gaatccggcc gccgctgcgg ccaggagggc ccccttgttg cgcagatcgc
      481 cccgggacgc caccagcagc gtcgccacca gttgcccggc acccgccatg gcggtaagat
      541 agcccaaccc cgtgcctccc actttgagca cgtcccgcgc catgacgggc atcatggcct
      601 gatagccgaa accaaacgcc tcgaccacca ggctgagcat gaggagcgtc cggatctcgc
      661 ggacgcgcaa cgcgtatccc agcccggcca tcaccgcccg gacaaatggc tgcgccgccc
      721 tcggggcggc ggcgggattc ctgaggaaga gcagcgcggc cgcgccgccg cagtaggcgg
      781 cacagaccag cacgtagttc ttcccgacgc ccaaccggtc gatgacgacg cccgcggcca
      841 gcgcgccgac gattctgacc aggctgccgg ccatgaactg cagggcgctc gcgttcaaga
      901 ggcgttcggg gcccaccacg tcgtacatca ggctgctcgc cgacggcttg gtgaccgcat
      961 tcacgagccc gatcagcgcc agatagcaca ggatgtgcca caggcgcgcc gcatgattga
     1021 ggaggagcac cgccagcgtc agggccccca gcgccgccgc gagttgtccg gcgacgacca
     1081 gccgtcggcg gtcgagccgg tccacgacgg ggccggccag cacgctgaac aacccctggc
     1141 tgaccccccg caaccccgcc gcgaggccga cccagaacgc ggagttggtc aactgcagga
     1201 ccacccaccc ctggctgagc aggtccaccg tgaaggccat cgcgctgaag agcgagtaca
     1261 gccacagcca gcggtaggcg ggcgcgtcaa acgcgaacga agagcgcaca ggccagagtt
     1321 ccgcaccccg cgcggcagtc ctccccaagg gcagcgtgaa accgatacta gggggtcgtc
     1381 ccctcccctc cgaatactcg actcagggcc gcgggaaggc tgcggcttcg ggcgaaggag
     1441 cgggtgtcga ctcgctgact gctcaccttc ttaatcgtca aggacctggg ctggctcaat
     1501 agctaccagg gcctgatcat tcgcgtggcc gccgacgcgt tcggcgtgct tctgatgcgg
     1561 cagttcatgc tccacctgcc ggactcgttg atcgaggcgg cccggatcga cggcgccagc
     1621 gagctcggca cctacttccg gatcgtggtc ccgctcatct ggcccgcggt cctgaccctg
     1681 acgatcttcg cgtggcgcga gacgtgggac gccttcgtgt ggccgttcat catcatcact
     1741 caggacagcc tccgcacggt gtccatcggg ctgcagcgct tcccggagga atacgtcaca
     1801 cctacaacga ggtcatggcg atctcgacca tcgcgatgat tccgatggtc ctgctgttct
     1861 tcttcttcca gcgcgctttc atccagggca tcgccctgtc tggcctcaag gaatagtggt
     1921 tcgcgcggct ccctcaagac agaacctgat aagcgtggag cgctccccgc acggcggata
     1981 gttcagcacc tagggaagtg gtcagccgat gaagtggctg aattctgcac tatctgctgt
     2041 ggtgtgtggg tcgcggtttc accctttatc atccccagcc tgaagtggag caatgtgatc
     2101 gtcggcatcg gggtcgccgt aacggcctac atggccatga gcgctaagac ctaactcgtc
     2161 cctaaaccgt cttctccgcg cgcagaagaa tcacacgggg gggactatat gtccgcatgt
     2221 gcaaatgccg gattaaatgt cttcgtgctt aaccgaaatc ggtggccgcg agatcttcgg
     2281 cctccgtccc gtcaagcccg gcccttgctg cttgcggctg tccaaccccc cggtgaagcg
     2341 gcacgtcccg aggaactttt agtttgagac ctagcacctc aacggggtgc ctgcgcggaa
     2401 accccgttcg ggatgacggc gatgccagtc acgatgtgca tattgtccat atcaacagtg
     2461 aaggacatcc catggtagta caggaccttg aaatgggacc attgaaaggt tctcagaggc
     2521 ctgccgagaa tgcctcggac actgtcttcc aacatgccga tgtgcaacag accgtcctgc
     2581 gtagcgtagt atacgt
//
LOCUS       DASQGH010000306         1996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40907, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000306 DASQGH010000000
VERSION     DASQGH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40907"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..643)
                     /locus_tag="VEW91_06050"
     CDS             complement(32..643)
                     /locus_tag="VEW91_06050"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018883773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYM91178.1"
                     /translation="MPPQLEPVTLVGARVKLVPMERSHVDALFRAGRSPEIWPYMPIW
                     VKTPEDMRRLVEEAVSAKEDGGELPLVIIDLGSGHVVGSTRLFEFSPAHRHVEIGWTW
                     LAPEAWRTSINTECKYLLLRHCFETLHLIRVQLKTDTRNLQSQRAIERIGAVREGVLR
                     HHRIMPDGYLRDSVYYSILAEEWPAVKARLEGWLGKGAATRSA"
     gene            771..1943
                     /gene="dinB"
                     /locus_tag="VEW91_06055"
     CDS             771..1943
                     /gene="dinB"
                     /locus_tag="VEW91_06055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYM91179.1"
                     /translation="MSPQDRVIVHVDMDAFYASIEARDNPQYRSVPVVVGAAPGGRGV
                     VAAASYEARRYGVRSAMPVGEAAARCPHAVFLPVDMAKYRAVSAQLFEVLDTFTPQVE
                     PVSIDEAFLDLTGCPLPARPDPPLEIGRAIKARIGEALRLPASVGVAPNKFLAKLASE
                     LAKPDGLRAIRAGEAVEVLAPLPVTVLWGVGAHTRERLHSLGIRTVGDLQQTPTSMLR
                     ASLGFAAEHLAQLCRGIDDRPVVVSRGAKSVGRETTFDQDTADRGVLDRALGVLAEDV
                     AHTLRAEGLRGRTVTLKLRYSNFHTLTRNLTLPAPTDSGATLFRTAVGLLDRLGTLPR
                     PVRLVGVSVSGLGRADLAQVPLFGDEDQQGRVDQLVDAINERFGEGTVRQPRGGDP"
BASE COUNT          291 a          681 c          701 g          323 t
ORIGIN      
        1 cctgcgaggt ccagcgaaaa gggcggcgcc gctatgcgga tcgggtggcc gcacctttcc
       61 cgagccagcc ctccagccgg gctttgaccg cgggccattc ctcagcgagg atgctgtagt
      121 agacggagtc gcgaaggtag ccgtccggca tgatccggtg gtggcggagc acgccttccc
      181 ggacggcccc gatccgttcg atcgctcgct gggactggag gttgcgcgtg tcggtcttca
      241 actgcacccg gatcaggtgg agcgtctcga agcagtggcg caggagcagg tacttgcact
      301 ccgtatttat ggaagtgcgc caggcttccg gcgccaacca ggtccacccg atctccacgt
      361 gtcggtgggc gggggagaac tcgaagaggc gcgtgctgcc cacgacatgg ccgctcccca
      421 ggtcgatgat gacaagggga agctcgcccc cgtcctcctt ggcgctgacc gcctcctcca
      481 ccagccgccg catgtcctcc ggcgttttga cccaaatcgg catgtaaggc cagatctccg
      541 ggctgcggcc ggcccgaaag agcgcgtcga cgtgcgagcg ctccatcggg accagcttga
      601 cccgggcccc caccagcgtc accggttcaa gttgcggagg cacgcctccc tcttcgcacc
      661 gagatccggc cgggcctgcg ggtttgacac cgcccggacc cgatctctac aatgggaaaa
      721 gcggctacga acgtctgttc gtatacgaca ccgcacacag gcgacggacg gtgagccctc
      781 aggaccgcgt catcgtgcat gtggatatgg atgcgttcta cgcctcgatc gaggcgcggg
      841 acaaccctca gtaccgcagc gttcccgtcg tcgttggggc cgcgccgggc gggcgtgggg
      901 tcgtggctgc ggcgtcgtac gaggcgcgca ggtacggcgt ccggtccgcg atgccggtgg
      961 gcgaagccgc ggcgcggtgc ccgcacgcgg tctttctgcc cgtggacatg gcgaagtacc
     1021 gggcggtgtc ggcgcagctg tttgaggtgc tcgacacgtt cacgccgcag gtcgagccgg
     1081 tctctatcga cgaggcgttc ctcgacctga cggggtgtcc gctgcccgcg cggcccgacc
     1141 cgccgcttga gattggccgc gccatcaagg cccggattgg agaggcgctg cggttgcccg
     1201 cctccgtggg ggttgcgccc aacaagtttc tggccaagct cgcctccgaa ctcgcgaagc
     1261 cggacggcct gcgcgcgatc cgggccgggg aggcggtgga ggtgctcgcc ccgttgcccg
     1321 tcaccgtctt gtggggggtt ggcgcgcaca cccgggagcg gctccactcc ctcggcatcc
     1381 gcacggtcgg ggacctccag caaaccccga catcgatgct gcgggcgtcg ctggggtttg
     1441 ccgccgagca cctggcccaa ctgtgccgcg ggatagacga ccggcccgtc gtcgtgtccc
     1501 ggggggccaa atcggtcggg cgcgagacga cgttcgatca ggacaccgcc gatcgggggg
     1561 tgctggaccg cgccctgggg gtgctcgccg aagatgtggc ccacaccctt cgagcggagg
     1621 ggctccgagg ccggaccgtg acgctcaaac tgcgctactc gaacttccac accctgacgc
     1681 ggaacctgac gcttcccgca ccgacggact ccggggccac cctgttccgc acagccgtgg
     1741 ggctcctgga ccgtctcggg acccttccgc ggccggtgcg gcttgtgggc gtgagcgtgt
     1801 cggggctcgg ccgcgccgat ctggcgcagg tgcccctgtt cggcgacgaa gaccaacagg
     1861 gtcgggtcga tcaactggtc gacgcgatca acgaacggtt cggcgagggc accgttcgcc
     1921 agccccgcgg cggggatcca taatccggcc cccggggggg cggctttagt ccgctgcctg
     1981 ggtatcctgc agcacg
//
LOCUS       DASQGH010000307         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38239, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000307 DASQGH010000000
VERSION     DASQGH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38239"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VEW91_06060"
     CDS             complement(<1..372)
                     /locus_tag="VEW91_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYM91180.1"
                     /translation="MYARERSLASWNIAVALVALAVGTWFGPLQALEHAGVNLYDSAG
                     GGVAYYQGLSIHGVFNALVWTTFFIIGFFTLTVTYGLQRPLVAPGLNVAGFVLMVVGT
                     VLTAAALLTNNASVLYTFYPPM"
     gene            complement(384..866)
                     /locus_tag="VEW91_06065"
     CDS             complement(384..866)
                     /locus_tag="VEW91_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYM91181.1"
                     /translation="MIPIDRYEKRWLAATGITLVVFVLAIAVTGFVVGVQLPSTAQRV
                     NPIRVSEQGPFATPGVRQLAPGRYDAYVIAPGMWTFYPNVIRIPQGSTVTFHLTSQDV
                     QHGFYVEGTNVHMMVLPGQVSELTTTFRKAGEYHFWCDEYCGLNHHTMSGRIIVEPKR
                     "
     gene            complement(863..1015)
                     /locus_tag="VEW91_06070"
     CDS             complement(863..1015)
                     /locus_tag="VEW91_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HYM91182.1"
                     /translation="MKNSRPQQTPGDDRHHAPQGTLALLLVYLVLIIVSWAGVYFVML
                     GRGVTR"
     gene            complement(1057..1710)
                     /locus_tag="VEW91_06075"
     CDS             complement(1057..1710)
                     /locus_tag="VEW91_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYM91183.1"
                     /translation="MPVGVWICGAFVTLLVCATLAFAVFRPVQVLPLLRPAPPVSLRD
                     ADGVPVTLGRLSGAIVVFQFSALRCAAPCPDGHQALQQLQQRAAAAPLRLPVRLVTVV
                     LDGQERPQALRARRAALGADSPWWQFATGDATQLKDAVGGGFGVYYAQDRDGRIALDP
                     ATVLVDERGIVRAEYRTAAPEAALIWRDIGLIAREASTRGAARALYSAAHLFLCYPR"
     gene            2037..2168
                     /locus_tag="VEW91_06080"
     CDS             2037..2168
                     /locus_tag="VEW91_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91184.1"
                     /translation="MAQQVLRGGTRPVLLIRLRNPSGTGEIGPWTPRSILVPLDGSG"
     gene            2155..2355
                     /locus_tag="VEW91_06085"
     CDS             2155..2355
                     /locus_tag="VEW91_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYM91185.1"
                     /translation="MGAGEPAQDVLDGALRVNADLLIMATHGRTGLDAVFSGSVASRI
                     GGRFPGPLLLVRSERAGRPGVP"
     gene            2728..>3387
                     /locus_tag="VEW91_06090"
     CDS             2728..>3387
                     /locus_tag="VEW91_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91186.1"
                     /translation="MSTVTTSIETVDAYVTASQRDPRLGRTTLHYIADMIDYFGKAKV
                     FEGIYGMDRQLDDIVAFFKGYAMSMERRLLLLVGPQGSGKSMTVDKLKRRLEEYSHKP
                     DGVVYAVKGCPFHQHPFDLLPPDVREEKGIWWHEEAVPCPVCERALAARGDWRPMPIQ
                     QLFISARDKVGVAKHTPTDLRREDITNFVGNINFAMLKERGSTYDPDAYDFEGKVIWA
                     NR"
BASE COUNT          619 a         1139 c         1110 g          519 t
ORIGIN      
        1 catcggcggg tagaacgtgt acagcacgct ggcgttgttg gtcaagagcg ccgccgcggt
       61 tagtaccgtg cccacgacca tcaggacgaa gcccgcgacg ttgagccccg gggcgactag
      121 gggccgctgg aggccgtagg tcaccgtcag cgtgaagaac ccgatgataa agaacgtggt
      181 ccacaccagc gcgttgaaca cgccgtggat cgagaggccc tgataatagg cgaccccgcc
      241 gcctgccgag tcgtacaggt tcaccccggc gtgctcgaga gcttgcagag gaccgaacca
      301 cgttccaacg gcaagcgcaa ccagcgcgac cgcgatgttc caggacgcca gcgatctctc
      361 ccgcgcgtac atcgtccgct cccctaccgc ttcggctcga cgataatccg accggacatg
      421 gtgtggtgat tcaagccgca gtactcatcg caccagaagt ggtactcgcc agcctttcga
      481 aacgtcgtcg ttagctccga tacctggccc ggcagcacca tcatgtgcac gttcgtcccc
      541 tccacgtaga agccgtgctg cacgtcctgg ctcgtcaggt gaaacgtgac cgtggacccc
      601 tgggggatgc ggatcacgtt cgggtagaac gtccacatcc ccggcgcgat cacgtacgcg
      661 tcgtaccgcc ccggtgcgag ctgccgcacg ccgggcgtcg cgaacgggcc ctgctccgac
      721 actcggatgg ggttcacccg ctgcgctgtg ctcggcagct gcacgccgac gacgaacccc
      781 gtgacggcga tcgcgaggac aaagacgacc agcgtaatgc ccgtcgccgc cagccagcgc
      841 ttctcgtacc ggtcgatggg gatcatcgcg tgaccccccg gccgagcata acgaaataca
      901 cgccggccca cgagacgatg atcaggacga ggtagaccag cagcagcgcc agcgtcccct
      961 gtggcgcatg gtggcgatcg tcacccgggg tctgctgcgg acgcgaattc ttcatcgggt
     1021 cccccttcgt cgtccggcca aatcaagctt gacctctcag cgcggatagc ataggaagag
     1081 atgcgccgcc gagtacaacg cgcgcgccgc cccgcgggtc gacgcctctc gggcgatgag
     1141 tcctatatct cgccagatca gcgccgcctc cggcgcggcg gttcggtatt ccgcgcggac
     1201 gatgccgcgc tcgtccacca gaaccgtcgc cgggtcgagc gcgatgcggc cgtcgcgatc
     1261 ctgtgcgtag tacacgccga acccgccacc cacagcgtct ttgagttgcg tcgcgtcgcc
     1321 ggtcgcgaac tgccaccagg gagagtctgc cccaagcgca gcgcggcggg cgcggagggc
     1381 ttgcggccgc tcctggccat cgaggaccac ggtgacgaga cgtacgggaa ggcgcagcgg
     1441 cgctgccgcg gcgcgctgct gcaactgctg cagtgcctgg tggccgtccg ggcacggggc
     1501 ggcacaccgc agcgcggaga attggaagac gacgatcgcg ccactcaacc gccccaaggt
     1561 gaccggcacg ccgtccgcgt cgcgcagcga tacgggaggc gccggccgga gcaacggcag
     1621 cacctgcacg ggccggaaca cagcgaacgc cagggttgcg cagacgagca gggtgacgaa
     1681 cgccccgcag atccagacac cgaccggcat cggccgctcg tgcgcgtggg gcgcggcggc
     1741 cgcccttgca gggcgagcca atgattctcc gttggaaccg agcgtggtca ttgacaggcc
     1801 tcaaccgcct gatgccgatg gtcgcgcacg tactcaacgc cgctcatttc tcaaatgctc
     1861 atcgaatttc ctctcattcg catcagtatt cgcgatcagt tcgattaaga ctgagcaacc
     1921 aaaggccagg tctgtggcca agagcatcat cgagcatgcc gctgatctca gcgccgatct
     1981 gatcatccct tccacccaac ggcgggggag gcgcgcggcg ggtcctcttc ggcagcgtgg
     2041 cccagcaggt gctccggggc ggcacccggc cggtgctgct catccgcctc cgcaacccga
     2101 gcgggaccgg ggagatcggc ccctggaccc cgaggagcat cctcgtgccg cttgatggga
     2161 gcgggtgagc cggcccagga cgtcctggat ggggcgctcc gggtgaacgc cgatctgttg
     2221 atcatggcca cccacggccg gaccggcctc gacgccgtct tctccgggag cgtcgcctcg
     2281 aggatcggcg gcaggtttcc gggcccgctg ctcctggtgc gatccgagcg ggcgggcagg
     2341 cccggggtcc cctgacctgc gtgggaccct ccggtcgggc gaggaaccgg cacccgcctc
     2401 cccgacgccc tgatttgaca ggttcccggg gtgcggctat actcggctca aagttcgccc
     2461 ccttgggcac atagaatgta gcaggcatcg gcgcatacaa cctcgcatcg ggagacggcg
     2521 cagcaccgga ccaactgacg tggcaacagg ggcgcggcaa agaggaggaa cccagacggg
     2581 aggtttctgt tcccgcactg ggtttttgct tttgtgggag atcccggagg ctcggccggg
     2641 ggtgtctggg gcacagatac caataaggat gcaggcgaac cgggacggtc gctgccgcgt
     2701 acattggaca aaggatggcg agagggcatg agcactgtca caaccagcat cgagaccgtt
     2761 gacgcctacg tcaccgcgag ccagcgcgac ccgcgcctgg gccgcaccac cctccactat
     2821 atcgccgata tgatcgacta cttcggcaag gcaaaggtct tcgaaggcat ctacgggatg
     2881 gaccggcaac tcgacgacat cgtggcgttc ttcaaaggat acgccatgag catggagcgc
     2941 cgcctgctcc tgttggtggg cccgcagggc tccggcaagt cgatgacggt ggacaagctc
     3001 aagcggcggc tcgaggagta ctcgcacaag cccgacgggg tggtgtacgc ggtcaagggg
     3061 tgtccgttcc accagcatcc cttcgacctg ctccccccgg acgtgcggga agagaagggc
     3121 atctggtggc acgaggaagc ggtcccctgc cccgtgtgtg aacgggcgct cgccgcgcgc
     3181 ggagactggc gcccgatgcc catccagcag ctcttcatct ccgcccgcga caaggtcggg
     3241 gtggccaagc ataccccgac cgacctgcgc cgcgaagaca tcaccaactt cgtcggcaac
     3301 atcaatttcg ccatgctgaa ggagcggggc tcgacctacg atcccgacgc gtacgacttc
     3361 gaggggaagg tgatctgggc gaaccgg
//
LOCUS       DASQGH010000308         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32542, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000308 DASQGH010000000
VERSION     DASQGH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32542"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..832
                     /locus_tag="VEW91_06095"
     CDS             119..832
                     /locus_tag="VEW91_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HYM91187.1"
                     /translation="MHIVHSDPTGRTAGQYARATVRRLRMRTLVPLGALAVATALLGR
                     TFGLHDARFLASEIALLVSMFVISRYVLPLVERRDRGAAGEEHVGALLEELSDGGWRV
                     IHDASLGRGNVDHILIGPAGVFTVETKSHPGPVRVGRVHGATIAQAQAQRRMIEGVAG
                     TKVEPLIVFSRAWVDRPMARRKGVRIVPARMLLGYLERLPATLSSGEIEKVRGLVAEA
                     LLERAARGGAGQERLRLIR"
     gene            898..>1937
                     /locus_tag="VEW91_06100"
     CDS             898..>1937
                     /locus_tag="VEW91_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYM91188.1"
                     /translation="MATTTTPRAGRGQLQAAGAPGAGEPRRLPPGPRAPAFVQTLAWA
                     VAPTWVMDRCARRLGEAFTLTFAPSGMQLVMISDPAAVKTVFTAPPEVAPSAVGNSPV
                     APVMGPSSVLVLTGPEHMRQRKLLLPPFHGERMREYEQVIVEATRRDMASWPLGRPMR
                     LQERTRAITLEVILRAVFGVEAERMDPLRAAIAGLMAPMNPLRILLFALRRPSLERPT
                     GAIGEGLDGLDAVIYAELARRRAQPDLAERTDILSLLMQARDEEGREMTDGELRDELV
                     TLLLAGHETTATAVAWAIERLVRHPEKLERLGAEIDAEGEGGGDEYMRAVVNETLRVR
                     PVVPMVARVLTE"
BASE COUNT          274 a          564 c          847 g          252 t
ORIGIN      
        1 aggctttaga acacgggggc agtctccacg cttctccagg ctgagcaccc ggtgaaccac
       61 gctcaagccg gcggcggcga acggccgatg tagaaggaaa gacgacgata agggagagat
      121 gcacatagtc cactccgacc cgaccggccg cacggccgga cagtacgcac gcgcgacggt
      181 gaggcgcctg cgcatgcgca cgctggtgcc gctcggggcg ctggcggtgg cgacggcgct
      241 gctgggccgg acgttcggat tgcacgacgc ccgcttcctc gcctcggaga tagcgctgct
      301 ggtgagcatg ttcgtgatct cccggtacgt gctgccgctg gtggagcgcc gcgaccgggg
      361 ggcggcggga gaggagcacg tgggagccct gctggaggag ctctcggacg gcggctggcg
      421 ggtgatccac gacgcgagcc tggggcgggg caacgtcgat cacatcctga tcggcccggc
      481 gggggtgttc acggtcgaga cgaagagcca tcccgggccg gtgcgggtgg ggcgggtgca
      541 cggcgcgacg atcgcccagg cccaggccca gcgcaggatg atcgaagggg tggcggggac
      601 gaaggtggag ccgctgatcg tgttcagccg ggcgtgggtc gacaggccga tggcccggcg
      661 caagggcgtg cggatcgtcc cggcgcggat gctgctgggc tacctggaga ggctcccggc
      721 gacgctgtcc tcgggcgaga tcgagaaggt cagggggctg gtggcggagg cgctgctcga
      781 gcgggcggcg cggggtgggg ctggacagga acgcttgcgc ctgattcgct gacggcgggc
      841 ggccgcgcgg gccgtgttgt caaggccaga ggtggcccat gggggtaccg taggaggatg
      901 gcgacgacga ccacgccgag ggccggccgc gggcagttgc aggcagcggg ggcgcccggg
      961 gccggcgagc cgcggcggct gccgccgggt ccgagggcgc cggcgttcgt gcagacgctc
     1021 gcgtgggcgg tggcgccgac gtgggtgatg gaccgctgcg ccagaaggct gggggaggcg
     1081 ttcacgctga cgttcgcgcc gagcgggatg cagctggtga tgatcagcga cccggcggcg
     1141 gtcaagacgg tgttcacggc cccgcccgag gtggcgccgt cggcggtggg caactcgccg
     1201 gtggccccgg tgatggggcc gagctcggtg ctcgtgctga cgggtccgga gcacatgcgc
     1261 cagcgcaagc tgctcttgcc gccgttccac ggggagcgga tgcgggagta cgagcaggtg
     1321 atcgtggagg cgaccaggcg ggacatggcg agctggccgc tgggccggcc gatgcgcctg
     1381 caggagcgca cccgggcgat cacgctggag gtgatcctgc gggcggtgtt cggggtggag
     1441 gccgagcgga tggaccccct gagggcggcg atcgcgggcc tgatggcgcc gatgaacccg
     1501 ctgcggatcc tgctgttcgc gttgcgccgg ccgagcctgg agcgtccgac gggggcgatc
     1561 ggggaggggc tggacggcct ggatgcggtc atctacgcgg agctcgcccg ccgtcgcgcc
     1621 cagccggacc tggcggagcg cacggacatc ctgtcgctgc tgatgcaggc gcgggacgag
     1681 gaggggcggg agatgacgga cggtgagctg cgggacgagc tggtcaccct gctgctggcg
     1741 ggccacgaga cgacggcgac ggcggtggcg tgggcgatcg agcggctggt gcgccacccg
     1801 gagaagctgg agcggctggg ggcggagatc gacgccgagg gtgagggcgg cggcgatgag
     1861 tacatgcggg cggtggtgaa cgagacgctg cgggtgaggc cggtggtgcc gatggtggcg
     1921 agggtgctga cggagga
//
LOCUS       DASQGH010000309         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55081, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000309 DASQGH010000000
VERSION     DASQGH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55081"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VEW91_06105"
     CDS             <1..703
                     /locus_tag="VEW91_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91189.1"
                     /translation="TKRFGGLLAVQDITVEIAPGEITGLIGPNGAGKTTLFKVINGSL
                     PATAGRVRLGDLEITGWSPHAICRLGVCHTHQIVRPFLDLTVLENVLVPAAFGRGMRA
                     RGPARAAAEEALAFTGLSSRPHVPASALTLAERKRLEIARALATRPTVLLLDEVLAGL
                     NPAESERAVTLVRQIKARGVTIVMIEHVMRAVMALSDRVLVLNYGRLIADGPPAEVAN
                     LPQVIEAYLGQRIEG"
     gene            717..1421
                     /locus_tag="VEW91_06110"
     CDS             717..1421
                     /locus_tag="VEW91_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91190.1"
                     /translation="MLEIAAIVAAYGEVRALWDVSLVVNEGEIVTLLGANGAGKTTTM
                     RVLSGLLRPLSGTVTFAGERIHRLPPPRIVQAGLVQIPEGRRLWPRMTVLENLELGAY
                     APHPRARRHESLDWVFSLFPRLAERRTQLAGTLSGGEQQMLAIGRGLMSRPRLLMLDE
                     PSLGLAPILVREVFNIISQINARGVTVLLVEQNVRQALEIAHRGYVLETGRIVRSGPA
                     RDLLEDPEIKRVYLGL"
     gene            complement(1396..2802)
                     /locus_tag="VEW91_06115"
     CDS             complement(1396..2802)
                     /locus_tag="VEW91_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYM91191.1"
                     /translation="MKPPLRIDDALFQVPETAALRRAAVQILARALREVDAYTVTSRA
                     IRLERDALLVDGRRFPLPSGGRVIVVGAGKACAPMARAVEDILGDRVTAGLVTVKTGY
                     TAPLRLITLREASHPLPDAAGEAAARETLALLSGLGPGDLVICLISGGGSALLPLPRE
                     GLTLRDKVDTTDLLLRSGADITEINTVRKHLSRIKGGQLARAAAPARLVAVVVSDIVG
                     SPLDAIASGPTVPDGTTFADALAVLEKYRLTARVPPPVLATLRRGAAGGLAETPKAGD
                     AAFTHTFTTVVADNAAAARAAAAEAERLGFRVLLLSTYIEGEAREVGRALGGIAREIA
                     ATGHPVARPACIVCGGETTVTVTGAGRGGRNQEVALGAARPLAGLPGALLASFATDGT
                     DGPTDAAGGVADGTTLARARARGLDAARHLADNNAYPLLEAVGDLIRTGPTNTNVNDL
                     MLILCGEGAEPTGPDRPA"
BASE COUNT          406 a         1023 c         1043 g          420 t
ORIGIN      
        1 gacgaagcgg ttcggggggc tcttggcggt ccaggacatc acggtggaga tcgccccggg
       61 agagatcacc ggcttgatcg gtcccaacgg ggccggcaaa accaccctgt tcaaggtcat
      121 taacggcagc ctgcccgcca cggcgggccg ggtcaggctg ggagacctgg agatcaccgg
      181 ctggagcccc cacgcgatct gccgcctcgg ggtctgccac acccatcaga tcgtccgccc
      241 cttcctcgac ctcacggtcc tggaaaacgt gctggtgccc gccgcgtttg ggcgggggat
      301 gagggcccgg ggccccgccc gcgcggcggc cgaggaggcg ctggcgttca ccgggttgtc
      361 ctcccggccc cacgtcccgg cgtctgccct caccctcgcg gaacgcaagc ggctggagat
      421 cgcccgggcg ctggccaccc ggcccaccgt cctgctgctg gacgaggtgc tggccgggct
      481 caacccagcg gagtccgagc gggccgtgac cctcgtcagg cagatcaagg cccgcggcgt
      541 gaccatcgtc atgatcgagc acgtcatgcg ggcggtgatg gcgctgtcgg accgcgtcct
      601 cgtcctgaac tacggccggc tcatcgccga cgggcctccc gccgaggtgg ccaacctccc
      661 ccaggtcatc gaggcgtacc tggggcagcg gatcgagggg tagcggggtg ggcgcgctgc
      721 tcgagatcgc tgccatcgtc gccgcctatg gcgaggtgcg cgccctctgg gacgtctcgc
      781 tcgtggtcaa cgaaggggag atcgtcacgc tgctgggcgc caacggcgcc gggaagacga
      841 ccacgatgcg cgtcctgtcg gggctgctgc ggcccttgag cggtaccgtg acatttgcgg
      901 gcgagcgcat ccaccggctg ccgcccccgc gcatcgtgca ggccggcctg gtgcagatcc
      961 cggaagggcg gcggttgtgg ccgcgcatga ccgtgctcga gaacctcgag ctgggggcgt
     1021 acgcgccgca cccgcgcgcc cggcggcacg agtcgctgga ctgggtgttt tccctctttc
     1081 cccggctggc cgagcggcgc acgcagctgg ccggcacgct ctccgggggc gagcagcaga
     1141 tgctggcgat cggccggggg ctcatgagcc ggccccgcct cctgatgctg gacgaaccgt
     1201 cgctcgggct cgccccgatc ctcgtccgcg aggtcttcaa catcatcagc cagatcaacg
     1261 cccgcggggt gaccgtgctg ctcgtcgagc agaacgtgcg gcaggccctc gagatcgcac
     1321 accggggcta tgtgctggag accggtcgga tcgtccggtc tgggccggcg cgcgatctgc
     1381 tggaggatcc cgagatcaag cgggtctatc tgggcctgta ggctcggccc cctctccaca
     1441 gaggatcagc atcaggtcgt tgacgttcgt gttcgtcggg ccggtgcgga tcaggtctcc
     1501 gaccgcctcg agcagtgggt aggcgttgtt gtccgcgagg tggcgggccg cgtccaaccc
     1561 acgcgcgcgg gcccgggcca gggtggtacc gtcggcaacc cccccggccg catcggtcgg
     1621 gccatcggtc ccgtccgtgg caaagctcgc gagcaacgcc cccggaagcc cggcaagggg
     1681 gcgcgccgcc ccgagcgcca cttcctggtt gcgcccgccc cgccccgctc cggtcaccgt
     1741 caccgtcgtc tcgcccccgc agacgatgca cgcgggtcgg gcgacggggt gcccggtcgc
     1801 cgcgatctcg cgcgcaatcc cgccgagcgc ccgtcccacc tctctggctt ctccctctat
     1861 ataggtagaa agcaagagca cgcgaaaccc aaggcgctcg gcctccgcgg ccgcggcccg
     1921 cgccgcggcc gcgttgtcgg cgacgacggt ggtaaaggtg tgtgtaaacg cggcgtcccc
     1981 cgctttgggg gtctcggcca gccctccggc cgccccgcgc cgcaacgtgg ccaggacggg
     2041 gggtggaacg cgggcggtga gccgatactt ttcgaggacg gcaagcgcgt ccgcgaacgt
     2101 cgtcccatcc gggaccgtgg ggccggaggc gatcgcatcg agcgggctcc cgacgatatc
     2161 cgagacaacg accgcgacga gcctggcggg ggcggcggcg cgggccagct gtccgccttt
     2221 gatcctggag agatgcttgc ggacggtgtt gatctccgtg atgtccgccc cggagcggag
     2281 aagcaggtcc gtggtgtcca ccttgtcgcg gagcgtcagc ccctcccggg ggagggggag
     2341 gagggccgac ccgccgcccg agatcaggca gatcaccaga tcgccggggc cgaggccgga
     2401 gaggagcgcg agggtttccc gagccgccgc ttcccccgcc gcatcgggca ggggatggct
     2461 ggcctcccgc agggtgatca gccgaagcgg ggcggtgtac ccggttttga cggtgacgag
     2521 cccggccgtc acccggtctc ctaggatatc ttcgacggcc cgggccatcg gggcgcacgc
     2581 cttgccggcg ccaaccacga tcacgcgccc gccggaaggg agggggaacc gccggccgtc
     2641 gaccaggagc gcgtcgcgtt cgagcctgat cgcgcgcgac gtgacggtgt aggcatcgac
     2701 ctcgcggagc gcccgggcca gaatctggac ggcggcacgc cggagtgcag ccgtctccgg
     2761 gacctggaac agcgcgtcat cgattcggag cggaggcttc agggctaggt cacccgttca
     2821 actttcacga tgctcgatcc gcgcaccccc ttgaagaccg tgggatcgcc cagcactcgc
     2881 ccgaacacgg tc
//
LOCUS       DASQGH010000310         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46715, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000310 DASQGH010000000
VERSION     DASQGH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46715"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(648..1412)
                     /locus_tag="VEW91_06120"
     CDS             complement(648..1412)
                     /locus_tag="VEW91_06120"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYM91192.1"
                     /translation="MRLLRTLGDARAPKTLLPAVLVRLGLGDAYGVLETPIGPAFVAY
                     NAQGVSAVSRGGSTEEFEATFQARFGRPVRRVDRLPAALEGRLRRALRGTGRRSVRFD
                     LRGVSEFERAVLRKALDIPRGEVRPYGWIAREIGRPAAVRAVGTALGRNPIPLLIPCH
                     RVVRSDGRIGEYAFGSKLKRTVLAAEGVDVEGLDALARAGIHYHGSDTTRIYCLPTCR
                     NARRISPKHRVAFRSAGEARAAGYRPCEVCRPALAS"
     gene            complement(1472..>1843)
                     /locus_tag="VEW91_06125"
     CDS             complement(1472..>1843)
                     /locus_tag="VEW91_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYM91193.1"
                     /translation="NVFRNRRRGKRPPVASLDREGEGGGVEVAEADETLLPEPAFDRA
                     ELRRVLAALVSALPPRYRVPVVLRHVQGAAYREIASILGQPIGTVKGNVHRGLRLLEA
                     GLTRQQCVAPARPRVGAQQGG"
BASE COUNT          287 a          646 c          605 g          305 t
ORIGIN      
        1 gcgggagcat ttgacaacac tcttggtctc tccagccacc tccagcgaac agcgtcctgg
       61 acaccccaac cagacttgta atgagggggt taccggttcg aatccggtcg agggctccgg
      121 gtttcttcgc cccacttcca ggcaaaacgc agatccaaca ggtgttctcg cgcgatcggg
      181 ccgtcatggg ggagggctca cgaacctgac ttgggatatc tatccggaag aatgaggtca
      241 ttgatccgaa ggaacggggg gggatagcag cctaagggcg catagcccta ggtgtgactc
      301 ctacctcgcg gcgttccacg caacgtaaac gcgccgtctg gttactctgg gctattgcaa
      361 tcgtttgtgc gtcgatttgg acccaggggc tgtttctcgc gctcagggtg ggtcccagcg
      421 agcaagtcgg ggcaccaaat cctatctctt gggacgacct gtcgagagcg gtcctcagca
      481 acccgttcca agcgttgccg cttctggtgg gttcttctcg cgctcatcga ttggaatcgt
      541 gggcatcctc gtccttccct ctgggattga tcgcggaggg cgctgttgtc ctgagtctct
      601 atcttcgcaa gaagcgctgg taatcgggcg ccccctgctg ctcccggcta cgacgcgagc
      661 gcgggccggc acacctcgca cggccgatag cctgccgccc gcgcctcgcc ggcggatcgg
      721 aatgcgacgc ggtgcttggg ggagatccgg cgagcattgc ggcaggtggg caaacagtag
      781 atccgtgtgg tgtcgctgcc gtggtagtgg atcccggcgc gcgccagcgc gtcgagcccc
      841 tcgacatcaa cgccctcagc ggccagtacg gtgcgcttga gcttgctccc aaaggcgtac
      901 tcgccgatgc gcccgtcgct tcgtacgacg cggtggcacg gaatgaggag cgggatcgga
      961 ttgcgcccca gcgccgtgcc caccgcccgc accgccgcgg gacggccgat ctcccgggcg
     1021 atccagccgt agggccggac ctccccgcgc gggatgtcga gcgccttgcg caacaccgcg
     1081 cgctcgaact cggacacgcc ccgcaggtcg aagcggaccg accgccgccc cgtaccgcgc
     1141 aacgcgcgcc tgaggcgtcc ctcgagtgcc gccggcagcc tgtccacccg gcggaccggg
     1201 cggccgaagc gcgcctggaa ggtggcttcg aactcctcgg tcgacccccc cctcgagacg
     1261 gccgacaccc cctgggcatt gtatgcgaca aaggcgggac cgatcggggt ctccagcacg
     1321 ccatacgcgt cgcccaggcc gaggcgcacc agcacggccg ggaggagcgt cttgggggcc
     1381 cgcgcatcgc cgagagttcg aagcaaccgc acgacccctc cccctggcgc cgcggcaccg
     1441 cgtccgtgcg agcctgcctg tgtcatggtc atcatcctcc ctgctgtgct ccgacccggg
     1501 gtcgggccgg agcgacgcac tgctgcctcg tcaagccggc ctccagcagc cggaggcccc
     1561 tgtggacgtt tcccttcacc gtcccaatgg gttgccccag gatcgacgcg atctccctgt
     1621 aggcggcacc ctgcacgtgg cggaggacca cggggacgcg gtaccggggc gggagcgcgc
     1681 tgaccagggc cgcgagtacg cgccgaagct cggcgcgatc gaatgccggc tccggcagta
     1741 gggtctcgtc ggcttccgcc acctccacgc cacctccctc gccttcgcgg tcgagtgaag
     1801 ccacgggcgg ccgcttcccg cgccggcggt tccggaagac att
//
LOCUS       DASQGH010000311         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40928, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000311 DASQGH010000000
VERSION     DASQGH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40928"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VEW91_06130"
     CDS             <1..935
                     /locus_tag="VEW91_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HYM91194.1"
                     /translation="WWTRLQIKFAPIDKVLAWREAMENQETTDAYARPTGDAETDALL
                     AQLRKDERSHALAAEELRRATPAAVPGEDAQGRLGRLLGRERWHRTGSSWISGAVYGA
                     NDGLAAVFGIVAGVSGATGGSSFVLTAGLAGAIASALSMAVGAYLAERSTSEVAAANI
                     SGERKELEEHPEEEKEELSLFYQLKGFDKARADALADEFARNPEAMLQALVTEELGGV
                     SAGGNPVQAAIAAGVSTGVGALIPVLPFFWLGGTPGVIWAAIVSLVAHFVVGAAKSLF
                     TLRSWWSSGLEMTVAGVIVGGATYVLGFLFRVSG"
     gene            1053..>1589
                     /locus_tag="VEW91_06135"
     CDS             1053..>1589
                     /locus_tag="VEW91_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYM91195.1"
                     /translation="MQSDIQDLSLVTRGWQRIGWADAEMPVLRLIRDRFARERPLRGI
                     RIGACLHVTSETANLMRTLQSGGASLALCASNPLSTQDDVAAALVEHAHLPVFARRGE
                     DRDTYYRHIDQVLATRPHLTMDDGADLVSVLHARPELLGDVIAGTEETTTGVIRLRSM
                     ARAGALRYPIVAVNDANTK"
BASE COUNT          224 a          535 c          587 g          243 t
ORIGIN      
        1 cgtggtggac ccgtctgcag atcaagttcg ccccgatcga caaggtgctc gcctggcgtg
       61 aagcgatgga gaaccaggag accaccgacg cgtacgcccg tcccacgggc gacgcggaaa
      121 cggacgcgct gctcgcgcag ctgcggaaag acgagcgctc ccatgcgctg gcggccgagg
      181 aactgcggcg agcgacgccc gcggcggtcc cgggtgagga cgcgcagggc cggttggggc
      241 gcctccttgg gcgggagcgg tggcaccgaa ccggctccag ctggatctcg ggcgccgtct
      301 acggcgcgaa cgatgggctc gccgccgtct ttggcatcgt ggcgggggtc tcgggggcca
      361 ccggggggtc gagctttgtc ctcacggccg gtctcgcggg cgcgatcgcc tcggcgctgt
      421 ctatggcggt cggcgcctac ctcgcggagc gctccacgtc cgaggtggcg gccgccaaca
      481 tcagcggcga gcggaaagag ctcgaagagc atcccgaaga ggaaaaggag gaactgtcgc
      541 tgttttacca gctcaagggg ttcgacaagg cccgggcgga cgccctggcc gacgagttcg
      601 cccgcaatcc cgaggccatg ctgcaggcgc tcgtcaccga agagctcggc ggcgtcagcg
      661 cgggagggaa tcctgtccag gccgcgatcg ccgcgggcgt cagcaccggg gtcggcgcgc
      721 tcatccccgt gctgccgttt ttctggctcg ggggaacccc cggggtgatc tgggccgcga
      781 tcgtctccct cgtcgcgcac tttgtcgttg gcgcggccaa gtcgctgttc acgctgcgga
      841 gctggtggtc gtccgggctg gagatgacgg tcgccggggt catcgtgggc ggcgcgacct
      901 acgtcctcgg gttcctcttt agggtctccg gctagcccgc atttggatcg cggacgcgca
      961 cgttttgtta cactagtgtg tgcgcgcggg gcgtgcgcgc tgggtgcgcc gccggggtag
     1021 actccctgcc gcgcggtcgg agggagcgca tggtgcagtc ggatatccag gacctgagcc
     1081 ttgtcacacg gggatggcaa cgcatcggct gggccgacgc cgagatgccg gtgctccggt
     1141 tgatccggga ccggttcgcc cgcgagcggc cgctccgggg gatccggatc ggcgcctgcc
     1201 tgcacgtgac gtccgagacc gcgaacctca tgcgcaccct ccaatccggg ggtgcgtcgc
     1261 tggcgctgtg cgccagcaac ccgctctcca cgcaggacga cgtcgcggcc gccctggtcg
     1321 agcacgcgca cctccccgtg ttcgcgcgcc ggggagagga tcgggacacc tattaccggc
     1381 acatcgacca ggtgctggcc acccgtccgc acctgacgat ggacgacggg gcggacctgg
     1441 tgtcggtcct ccacgcccgt cccgagctgc tcggggacgt gatcgccggg accgaggaaa
     1501 ccaccaccgg cgtgatccgg ctccgcagca tggcgcgggc gggcgccctc cggtatccga
     1561 tcgtggcggt caacgacgcc aacacgaag
//
LOCUS       DASQGH010000312         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52224, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000312 DASQGH010000000
VERSION     DASQGH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52224"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="iolM"
                     /locus_tag="VEW91_06140"
     CDS             <1..1171
                     /gene="iolM"
                     /locus_tag="VEW91_06140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="scyllo-inosose 3-dehydrogenase"
                     /protein_id="HYM91196.1"
                     /translation="MKGLQITAEWAPRPEYAVSEFERRTGKAVTGASVWRHPRVQLAE
                     VQEPPLGPKEVRLRPRACGVCGSDVHFYETDDDGYIRYPGLTKFPVVIGHEFSGQVVE
                     TGKDVRDLRPGDLVTVEEMIWCGECIPCRNGWPNQCLNLEEIGFTINGAMAEQLVVGA
                     KYCWPITALAEVYGSEEAACEAGALCEPTSVAYNGMFVRAEGFKPGGVVAVYGTGPIG
                     FAAIALARAAGASRVIAFEVSGVRQALARQVGADEVHSPAELAKAGSSPHAAVLQASS
                     GAGADMAVEAAGAMTRTIPEIEASLAIGGKVVIIGRAAERAPMYLEHFQTHAGQLYGA
                     QGHSGYGTFQNVIRLMASRRIDLRPVITSRFPLREGVAAIERASLRQDGKVMVKG"
     gene            1177..>1642
                     /locus_tag="VEW91_06145"
     CDS             1177..>1642
                     /locus_tag="VEW91_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM91197.1"
                     /translation="MHRRVMPLGLILLLLVGTAGVGATAAKPAALNVGVVSGVEIRGL
                     KAIAPMWARATGVRLNLIEYPYPSLYEKLVTAFQANAATFDVVMLDDPWMPKFGSEGW
                     LAPLDAAPFNLARDPDIFPVVYDLGSWPPPRGPIPPGEATKPRHLEAITLVGN"
BASE COUNT          258 a          531 c          620 g          233 t
ORIGIN      
        1 gatgaagggg ctccagatca ccgccgagtg ggctccccgc ccggagtacg ccgtgtccga
       61 gttcgaacgc cggaccggga aggccgtgac cggggcaagc gtctggcgtc atccccgggt
      121 ccagctggcc gaggtccagg agccgcccct cgggcccaag gaggtgcgcc tgcgccctcg
      181 ggcgtgcggg gtctgcggct cggacgtgca cttctacgag acggacgatg acggatacat
      241 ccgctacccc gggttgacga agttccccgt ggtcatcggc cacgagttca gcgggcaggt
      301 cgtcgagacc ggcaaggacg tccgggacct ccggccgggg gatctggtca ccgtggaaga
      361 gatgatctgg tgcggggagt gcatcccctg ccggaacggc tggcccaacc agtgcctcaa
      421 tctcgaggag atcgggttca cgatcaacgg cgcgatggcg gagcaactag tggtgggagc
      481 caagtactgc tggccgatca ccgcgctcgc cgaggtctac ggaagcgagg aagcggcgtg
      541 cgaggccggt gccctgtgcg agccgacgag cgtcgcctac aacgggatgt tcgtccgcgc
      601 ggaggggttc aagcccggcg gggtggtggc cgtgtacgga acgggcccga tcgggttcgc
      661 ggcgatcgcg ctcgcccggg cggccggcgc cagccgggtg atcgcgttcg aagtcagcgg
      721 ggtgcggcag gcgctggcgc ggcaggtagg ggcggatgag gtgcacagcc ccgcggagtt
      781 ggccaaagcc gggagcagcc cccacgcggc ggtgctgcag gccagctccg gcgccggagc
      841 ggatatggcc gtggaggccg ccggggcgat gacccggacg atccccgaga tcgaggcgtc
      901 gctcgcgatc ggcgggaagg tggtgatcat cggccgcgcg gcagaacgcg ccccgatgta
      961 tctcgagcac ttccagaccc acgccggcca gctctacggc gcgcagggcc actcgggcta
     1021 cgggacgttc cagaacgtga tccggctgat ggcgtcccgc cggatcgacc tccggcccgt
     1081 catcacgagc cggtttccgc tcagggaggg cgtggccgca atcgagcggg ccagcctcag
     1141 gcaggacggc aaggtgatgg tgaaggggtg agggagatgc atcgacgcgt gatgccgctc
     1201 gggttgatcc tgctcctgct cgtgggaacc gccggggtcg gagcgaccgc ggccaagccc
     1261 gcagcgctga acgtcggcgt ggtgtcgggg gtggagatcc gcggcctcaa agcgatcgcg
     1321 ccgatgtggg ccagggccac cggggtgcgg ctcaacctga tcgagtaccc gtaccccagc
     1381 ctctacgaga aactcgtgac ggcgtttcag gcgaacgcag ccacgttcga cgtggtcatg
     1441 ctggacgacc cgtggatgcc gaagttcggc tccgagggat ggctcgctcc cctcgacgcg
     1501 gcgccgttca acctcgcgcg cgatccggac atctttccgg tggtctacga tcttggctcc
     1561 tggccgccgc cgcgcgggcc gatccccccg ggggaagcga ccaagccccg gcacctcgag
     1621 gcgatcacgc tggtcggcaa cg
//
LOCUS       DASQGH010000313         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1340, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000313 DASQGH010000000
VERSION     DASQGH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VEW91_06150"
     CDS             <1..320
                     /locus_tag="VEW91_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM91198.1"
                     /translation="PLGRRLLAGLETLRDLPGVGDVRGFGLMCGVELVTDKRAKTPAL
                     GLGARVLQEARARGLLARLRVGSADPPVGDTICLAPPLVTPPEMVDRIVEILRAAIPA
                     ASR"
     gene            447..1466
                     /locus_tag="VEW91_06155"
     CDS             447..1466
                     /locus_tag="VEW91_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYM91199.1"
                     /translation="MPGRRRLRSPVPATHPFLLEWWTDLEGDDRGLNTRLAYVRDLKD
                     FAEYVGGNLEGAVPEDLIRFTRRMTEAGLSVATRARRITSLRQFYEWLRKRQGRPFSI
                     AQDLHPPRRPHRVHRWWTEDQVAQFRTAFPEGTPRVARDRAMAELGLMGLRVSEVVRL
                     DIERVVHLSDPDQASLVVLRKGNKEQVLPITADARDALLAWLAVRPPAPTTALFFRLP
                     FQPRRVRLHYASVEKIFKRYAAAAGVPIPEGIAFHHLRHTAGQQMATVGLGIEEAQRL
                     LGHANPATTQVYYEVTDQRLRRAAQRLRYRGKVPPAGGFSPPAHKVAARRRPRERPAP
                     QPGPA"
     gene            complement(1509..2654)
                     /gene="amrS"
                     /locus_tag="VEW91_06160"
     CDS             complement(1509..2654)
                     /gene="amrS"
                     /locus_tag="VEW91_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HYM91200.1"
                     /translation="MVEQRTLKIRDAQALPIAQVLRKFAREGFLYRRLEAGKVQCVAC
                     GHRCVIYDGLPGVCRVRFNEGGKLYVPWGYVGTVQLDPIEKKPFFHAYPGTAALSFGM
                     LGCDFRCPFCQNWQLSQVLRDAVATRLADFMPITPQEFVTLALRDRAKVIASTYNEPL
                     ITSEWAVALFQEGRKHGLVAGYVSNGNATPEVLDYIRPWVDLYKIDLKAMRESSYRML
                     GGRLETVLNAIRWARERGFWVEVVTLVIPGFNDSEDELRDAARYVVSVSPDIPWHVTA
                     FSPEYAMTDRDSTPPATLIRAAEIGCAEGLKFVYAGNLPGRVGPFEHTYCPRCSTLLI
                     ARDGVRAVRYRIVGGRCPDCGEAIPGVWWRNGEVPPHLAAAQSPDGH"
     gene            complement(2661..2810)
                     /locus_tag="VEW91_06165"
     CDS             complement(2661..2810)
                     /locus_tag="VEW91_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91201.1"
                     /translation="MPVAVAEEEVAVVEEEVAVVAEITLVDTEEGEEIESEDTEDDEA
                     EVGLR"
     gene            complement(3043..3264)
                     /locus_tag="VEW91_06170"
     CDS             complement(3043..3264)
                     /locus_tag="VEW91_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYM91202.1"
                     /translation="MAGPVTRVQGTVKWFNEEKGFGFIAVEGSEDVFVHHTGIRGLGH
                     KSLREGQRVELEIIAGPKGPRAGDVTPLD"
     gene            complement(3468..>3954)
                     /locus_tag="VEW91_06175"
     CDS             complement(3468..>3954)
                     /locus_tag="VEW91_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM91203.1"
                     /translation="IPVNLGAAVTKLMRASRTLTQKLGREPTQRELARATGLSVARVR
                     QILEIPQQPASLEAPIGEDEDTSLGSLVADQEALGPEQALALSMLKEQVQGLLATLTP
                     RERIIVRLRFGLGGKRPETLEEIGQRLKLTRERVRQIERRALQKLLQPAQAGQLGNFV
                     A"
BASE COUNT          604 a         1400 c         1285 g          665 t
ORIGIN      
        1 gcccgctcgg ccgccggctg ctggccggtc tggagacttt gcgcgacctc ccgggtgtcg
       61 gcgatgtgcg cgggttcggc ctgatgtgcg gggtggagct ggtcaccgac aagcgggcca
      121 agacccccgc tctcgggctg ggggcgcgcg tgctccagga agcccgagcg cggggcctgc
      181 tcgcgcgcct gcgggtcggg tcggcggatc ccccggtcgg cgacacgatc tgcctggccc
      241 cgcccctggt gacgcctccg gagatggtcg acaggatcgt ggagatcctc cgggccgcga
      301 tccctgctgc atcacgctga caccgccgcc gggggaggga tcgacgggga caggaaaacc
      361 gggaaatcgg gcgaacatcc cattcgcgcg gcaggaacgc tgccggatca cactagagat
      421 cgcagctttc gtcggaggag ggcatcatgc caggacggcg gcgcctgcgt tcgccggtcc
      481 ccgccaccca cccgtttctc ctggagtggt ggaccgatct tgagggggac gaccgcgggc
      541 tcaatacgcg cctcgcctac gttcgcgacc tgaaggactt cgccgagtac gtcggcggca
      601 acctcgaggg cgccgtcccc gaggacctga tccggttcac gcgccggatg accgaagccg
      661 ggctctccgt cgccacgcgg gcccgccgca tcacctccct gcgccagttc tacgagtggc
      721 tccgcaagcg ccaggggcgg ccgttttcga tcgcgcagga cctgcatccg ccgcgccgcc
      781 cgcaccgggt ccatcggtgg tggaccgagg accaggtggc ccagttccgg accgccttcc
      841 cggagggcac tccgcgggtc gcccgggacc gggcgatggc cgagttgggg ctcatggggc
      901 tccgggtcag cgaggtggtc cggctggaca tcgagcgggt ggtgcatctg tccgatcccg
      961 accaggcctc cctcgtggtg ctgcggaagg ggaacaaaga gcaggtcctc ccgatcaccg
     1021 ccgatgcccg cgacgcgctg ctggcgtggc tggcggttcg cccccctgcc ccgaccaccg
     1081 cgctcttctt ccggttgccc ttccagcccc ggcgggttcg cctccactat gcgagcgtgg
     1141 agaagatctt caagcggtac gcggcggcgg ccggggtccc gattcccgag ggcatcgcgt
     1201 ttcaccacct ccgccacacc gcgggccagc agatggccac ggtcgggctc gggatcgaag
     1261 aagcccagcg cctgctgggg cacgccaacc cggcgaccac ccaggtctac tacgaggtga
     1321 ccgaccagcg cctgcgccga gcggcgcagc gcctccggta tcgaggcaag gtgccgccgg
     1381 cggggggatt ctccccgccg gcgcacaagg tggcggcccg gcgcaggccg cgcgaacgtc
     1441 cggcgccgca gcccgggccc gcatagatcg acgccctctc gcgccccaag caccgccccg
     1501 agcgcgggtc agtggccatc cggcgactgt gctgcggcga ggtgcggcgg cacctcaccg
     1561 ttccgccacc agacgccggg gatggcctcg ccgcagtcgg ggcagcggcc gccaacgatc
     1621 cggtaccgga cggcccgaac cccgtcgcgg gcgatcagca gggtgctgca cctcggacaa
     1681 taggtgtgct cgaacggtcc gacccggccc ggcaggttgc cggcgtacac gaacttgagg
     1741 ccctccgcgc atccgatctc ggccgcacgt atcagcgtgg ccgggggggt ggagtctcgg
     1801 tccgtcatgg cgtactcggg agagaacgcc gtcacatgcc aggggatatc cggcgagacc
     1861 gacaccacat accgggcggc atcccggagc tcgtcttcgc tgtcgttgaa accagggatg
     1921 accaaggtga cgacctcgac ccagaagcct cgctccctcg cccagcggat cgcgttgagc
     1981 accgtctcga gccgcccgcc gagcatgcgg tagctgctct cgcgcatggc cttgaggtcg
     2041 atcttgtaga ggtccaccca gggccgaatg tagtccagaa cctcgggcgt cgcgttccca
     2101 ttggagacgt agccggcgac aaggccgtgc ttgcggccct cctggaaaag cgcgaccgcc
     2161 cactcgctgg tgatcaaggg ctcgttgtag gtgctggcga tgaccttggc acggtcacgg
     2221 agggcgagcg tcacgaactc ctgcggggtg atcggcatga agtcggccag ccgcgtcgcc
     2281 acagcgtcgc ggaggacctg ggacagctgc cagttctggc agaacggaca ccggaagtcg
     2341 caacccaaca tcccgaagct gagggccgct gtgccggggt aggcatgaaa gaatggcttt
     2401 ttctcgatgg gatcgagctg gacggtgccg acgtaccccc acggcacgta cagcttccca
     2461 ccctcattga accgaacccg gcaaactccg gggagcccgt cgtagatgac gcagcgatgt
     2521 ccgcaggcca cacactggac ttttccggcc tcaagtcgtc ggtagagaaa cccctcccga
     2581 gcgaatttgc ggaggacctg cgcgatcggc agcgcctggg cgtccctgat cttcagggtt
     2641 cgctgctcga ccaccgccgt ttaccgaagc ccgacctcgg cctcgtcgtc ctcggtgtct
     2701 tcggattcga tctcctcacc ctcttccgtg tcgacaagcg tgatctccgc tactaccgca
     2761 acttcttcct ccacaaccgc gacttcttcc tccgcaaccg caaccggcac gggcggcttc
     2821 cgcgccttcg gcctggcggc ccgccgaccg gcaggcggct tgcggatcct cgggcgccgc
     2881 cgggctcttg tggtgcgctt cggggaagcc gccctcgccc cggtcatgcg ccgccccggc
     2941 cttcgcattg tacatccccc tctccccgag ttcgcgagac accgggcgcc cgcgtcacct
     3001 cagactccct ggcgcccgat gttggaaaaa tgcgctcagc agtcaatcaa ggggagtgac
     3061 gtctcccgcc cgagggccct tcggtcccgc gatgatctcg agttctaccc gttggccctc
     3121 acgcaggctc ttgtggccca agcccctgat cccggtgtgg tgaacaaata catcctcgct
     3181 cccctccacc gcaataaagc caaagccttt ttcctcgttg aaccacttaa cggttccctg
     3241 gacccttgtc accggccctg ccattccaat tcctccctcg gcctacatca cgcttcccat
     3301 tcatcttcag gatatccgtg ccttgtccca gagacaatcg ggcgtgcaca gtattagaac
     3361 cgcgggactg cgccctggct cggcggcgag agttcaatgc agtggctccg ctaccgcatt
     3421 cacggtgtgc gcgttgttgg ccccgacaga caggccgtgt gctggggtta cgcaacgaag
     3481 ttccccagtt gtccggcctg cgcaggctgg agcagcttct gcaacgcccg ccgctcgatc
     3541 tggcggacgc gctcccgggt cagcttgagc ctctgcccta tctcctccag cgtctctggc
     3601 cgtttcccgc cgagcccgaa gcgaagcctc acgattatcc tctcgcgtgg cgtgagggtt
     3661 gccaggaggc cctgcacttg ctctttgagc atcgacaggg cgagcgcttg ctctggcccg
     3721 agggcttctt gatccgcgac gaggctgccc aaagaggtat cctcgtcctc cccgatcggc
     3781 gcctcgagcg acgcgggctg ctggggaatc tccagaattt ggcgaactcg tgctaccgag
     3841 agcccagttg cccgggcgag ttctctttga gttggctccc gcccgagttt ctgggtgagg
     3901 gtgcgcgatg cccgcattag cttggtgacg gccgcgccga gattgacggg gatg
//
LOCUS       DASQGH010000314         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7051, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000314 DASQGH010000000
VERSION     DASQGH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7051"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VEW91_06180"
     CDS             <1..325
                     /locus_tag="VEW91_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91204.1"
                     /translation="TAAAPAIVRFVGGTPADALEVTRQMYRITLARHPGYTIAALGFA
                     GTPTAIDARRVVEQGILPVINTGIAHRKPGVGQIGAGIARAPLACFVDAVRALGRRLD
                     IEGAA"
     gene            325..1284
                     /gene="arcC"
                     /locus_tag="VEW91_06185"
     CDS             325..1284
                     /gene="arcC"
                     /locus_tag="VEW91_06185"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HYM91205.1"
                     /translation="MDRRAVIAVGGNALILDGQRGTIAEQFENARATVRHIAALVANG
                     WKVVVTHGNGPQVGFILLRSELVGDSAPVPQLSLDMCVADSQGGIGYILGSSLASELG
                     RLGLRDRVACILTHTVVAGGDPAFRTPSKPIGPWYSADQAAVRQQREGWAMVEDAGRG
                     YRRVVPSPQPLRIVEALQIRALLEAGFVVVAAGGGGIPVVEREPGSYQGVEAVIDKDL
                     ASARLALALEIPLLIICTGVDQVALHYRKPDQRFLDRLTVSEARRCLETGEFPPGSMG
                     PKMEAAIRFLAGGGKEVLITSPYRLEEAVAGGTGTRVVPDGRA"
     gene            1281..2036
                     /locus_tag="VEW91_06190"
     CDS             1281..2036
                     /locus_tag="VEW91_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HYM91206.1"
                     /translation="MNLRRATQADEGGSQVITVRAEPYDFEFDPRTAALLIIDMQRDF
                     VYPGGFGEALGNDTSLLLKAVPPTERVLKAARGAGMVVVHTREGHRPDLSDLARAKKA
                     RGRLKVGIGDPGPMGRILVRGEYGHDIVDELRPAPGEPVVDKPGKGSFYATDLDAILR
                     TRGIQQLIVCGVTTEVCVHTTVREANDRGYDCLVLEDCVGSYFPEFQEFGLKMIKAQG
                     GIFGWVSDSSRFLEAFAKAQAAQGARSTRADAR"
BASE COUNT          324 a          708 c          815 g          326 t
ORIGIN      
        1 gacggcggcc gcgccggcga tcgtgaggtt cgtggggggc actccggccg acgccctgga
       61 ggtgacgcgc cagatgtacc ggatcaccct ggcccgccat ccagggtaca ccatcgccgc
      121 cctcggattc gcggggaccc ccacggccat tgacgcgagg cgggtggtcg aacagggaat
      181 tctgccggtg atcaacaccg gcatcgcgca ccgcaagccc ggcgtgggtc agatcggggc
      241 cggcatcgcg cgcgcgccgc tcgcctgctt cgtggatgcc gtccgggcgc tcgggcggcg
      301 gctcgacatc gagggcgcgg cctgatggac cgccgcgcgg tcatcgcggt ggggggcaac
      361 gcgctgatcc tggacgggca gcgggggacc atcgcggaac agttcgaaaa cgcgcgcgcc
      421 acggtccgcc acatcgcggc gctggtggcc aacggctgga aggtcgtcgt cacgcacggg
      481 aatgggcccc aggtcgggtt catcctgctc cggtcggaac tggtcgggga ctcggcaccg
      541 gtcccgcagc tctcgctgga tatgtgcgtg gccgactccc agggggggat cggctacatc
      601 ctgggcagct cgctcgccag cgaactcgga cggctcggcc tccgagaccg ggtcgcctgc
      661 atcctcaccc acaccgtggt ggccgggggc gatccggcct tccggacgcc gagcaagccc
      721 atcggcccct ggtactccgc cgaccaggcg gcggtgcggc agcagcggga aggctgggcg
      781 atggtcgaag acgcgggccg cggctaccgg cgcgtcgtcc cgtcccccca gcccctccgg
      841 atcgtcgagg cgctccagat ccgcgccctg ctggaggcag ggttcgtggt cgttgccgcc
      901 ggcggcggcg gcatccccgt ggtggaacgc gagccgggca gctaccaggg ggtggaggcg
      961 gtgatcgaca aggatctcgc gtcggcgcgg ctggcgctgg cgcttgagat cccgctgctc
     1021 atcatctgca ccggggtgga ccaggtcgcc ctccattacc gcaagcccga ccagcggttc
     1081 ctcgaccggc tcacggtctc ggaggcgcgg cggtgcctgg agaccggcga gttccctccc
     1141 ggcagcatgg ggccgaagat ggaggccgcg atcaggtttc tggccggcgg cggaaaggag
     1201 gtactgatta cctcgccgta ccggctcgaa gaggctgtgg cgggtgggac cggcacgcgg
     1261 gtcgtgcccg acggccgggc gtgaatctgc ggcgcgcgac ccaggcagat gaagggggga
     1321 gccaggtgat cacggtccgg gcagaaccgt acgacttcga gttcgatccg aggacggccg
     1381 ccctgctcat catcgatatg cagcgggatt tcgtctaccc gggcggcttc ggtgaggcgc
     1441 tcgggaacga cacgtcattg ctcctcaagg ccgtgccgcc aaccgagcgc gtgctcaagg
     1501 cagcccgggg ggcggggatg gtcgtcgtgc acacccgcga aggacaccgc cccgacctct
     1561 ccgacctggc gagggccaag aaggcgcgcg gtcgcttgaa ggttgggatc ggggatccgg
     1621 ggccgatggg gcgcatcctg gtccgcggcg agtacggcca cgacatcgtg gacgaactcc
     1681 ggccggcccc cggagaaccg gtggtggata agcccgggaa aggctccttc tacgcgaccg
     1741 acctggacgc gatcctgcgc acccggggga tccagcagct catcgtctgt ggcgtcacca
     1801 ccgaggtgtg cgtgcacacc accgtgcgcg aagccaatga ccgcggttac gactgcctgg
     1861 tcttggagga ctgcgtcggc tcgtactttc ccgaattcca ggaattcggc ctcaagatga
     1921 tcaaggccca gggcgggatt ttcggctggg tgagcgactc gtcgcgcttc ctggaggcat
     1981 tcgcgaaggc gcaggctgcg caaggggcga ggtccacgcg ggctgacgca cgctgaaccg
     2041 gggggagggg tggggcatgg cggcatcggc gggcgagttt cgaccgcggc tgtgggtcac
     2101 cggggactgg aacgcctttt tcgggttgtt cacgaacgtg gcgctgaacg tgatcgtcct
     2161 gtcgagtctg gtc
//
LOCUS       DASQGH010000315         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40964, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000315 DASQGH010000000
VERSION     DASQGH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40964"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..>1569)
                     /locus_tag="VEW91_06195"
     CDS             complement(40..>1569)
                     /locus_tag="VEW91_06195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYM91207.1"
                     /translation="SVETGSGTEAAGVPWGSPLAGSGSMAGSRGFLGLPTRRRLVGTG
                     IAEVRGPAACMARDALFRRMLLLADMVAIVGAFVLTVELSRRPLQLTWAAAAGVPILV
                     VCAKLTGLYDRDETLLRKTTLDEAPKLFQLATLCALVTWLTGGLITHSVIDRHEALFL
                     WLALAGGLILARAVSRALALRIAPTERCLFIGDEVSAETIRSKLTDHGGVKAEVVAHL
                     DLDKVASWSTDTFSEARLAEIRDLAQTLDVHRAIIAPRSVDGGEMLNLVRTLKAVGVR
                     VSLLPRLLEVVGSSVEFDDLHGVTVMGVRRFELTRSSAAFKRAFDLFGASLGLLAVSP
                     LLAVIAVAIKLDSRGPVFFRQLRVGRHGHRFQMLKFRTMVPEAEAMKDSLRHRNEARE
                     GLFKIAEDPRVTRVGRLLRCTALDELPQLWNIVRGEMSLVGPRPLVVDEDQRVEGWHR
                     RRLELMPGMTGPWQILGPARVPLKEMVAIDYIYIANWSLWTDLKILLRTVPHVLGRRG
                     L"
BASE COUNT          265 a          608 c          497 g          199 t
ORIGIN      
        1 ggcacgaggc cctcccacag ccccccgcga cgcggagccc tacagcccgc gacgcccgag
       61 cacgtgcggg acggtgcgca ggaggatctt caggtcggtc cacagcgacc agttggcgat
      121 gtagatgtag tcgatcgcca ccatctcctt gagcggcacg cgggcggggc cgaggatctg
      181 ccaggggccg gtcatgccgg gcatcagctc cagccggcga cggtgccagc cctcgacccg
      241 ctggtcctcg tcgacgacca gcggccgtgg cccgacgagg ctcatctcgc cgcggacgat
      301 gttccacagc tgcggcagct cgtccagggc ggtgcatcgc agcagccgcc cgaccctggt
      361 cacccgggga tcctcggcga tcttgaagag cccctcccgg gcctcgttgc gatgccgcag
      421 ggagtccttc atcgcctcgg cctccgggac catcgtgcgg aacttcagca tctggaagcg
      481 gtggccgtgg cgaccgaccc gcagctggcg gaagaacacc gggccgcggc tgtcgagctt
      541 gatcgccacc gcgatcacgg ccagcagcgg cgagacggcg agcagcccga gcgaggcgcc
      601 gaacaggtcg aaggcccgct tgaaggccgc cgaggagcgc gtcagttcga agcgcctgac
      661 ccccatcacg gtcaccccgt ggagatcgtc gaactcgacc gacgacccca ccacctccaa
      721 cagccgcggc agcaggctca cccgcacccc gaccgccttc agcgtgcgca cgaggttcag
      781 catctccccc ccgtccacgc tgcgcggcgc gatgatcgcg cgatgcacgt cgagcgtctg
      841 cgccaggtcg cggatctcgg ccagcctggc ctcggagaag gtgtccgtcg accaggacgc
      901 cactttgtcg agatccaggt gagcgactac ctccgccttg accccgccgt ggtcggtcag
      961 cttcgagcgg atcgtctccg ccgagacctc atcgccgatg aataggcacc gctccgtcgg
     1021 cgcgatccgc agggcgagcg cccgtgacac cgcccgcgca agtatcaacc ccccggccag
     1081 cgccagccac aggaaaagcg cctcgtgacg atcgatcacg ctatgcgtga tcaacccgcc
     1141 ggtgagccat gtcaccagcg cgcacaatgt ggcgagctga aacagcttcg gcgcctcgtc
     1201 gagcgtggtc ttgcgcagca gcgtctcgtc gcggtcatac aggccggtga gcttcgcgca
     1261 caccaccagg atcggcaccc cggcggcagc tgcccacgtc agctgcagcg ggcgacggga
     1321 cagctccacc gtgagcacga acgcccccac gatcgccacc atgtcggcca gcagcagcat
     1381 gcggcggaac agcgcatccc gcgccatgca ggccgccggc ccgcgcacct cggcgatgcc
     1441 cgtccccacc aggcgtcgcc gcgtcggcag gcccaggaac ccccgcgagc ccgccatcga
     1501 ccccgaaccg gccaggggcg atccccaagg aacccccgcc gcctcggtcc cactccccgt
     1561 ctcgacact
//
LOCUS       DASQGH010000316         7576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21151, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000316 DASQGH010000000
VERSION     DASQGH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21151"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VEW91_06200"
     CDS             <1..768
                     /locus_tag="VEW91_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018783997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91208.1"
                     /translation="TAAPAAAGAAFLVLLLLPMLSLLVRVPPAGLLPRLATPQVLQAL
                     WLSLGTSLAATVAVVGLGLPVAYLLAVRGFPGKRVLEALIDLPMVLPPTVAGVGLLLA
                     FGRAGIAGRALSALGITVPFTTLAVVLAQAFVAGPFFINAARAGFEEVDPRYLRAAAT
                     LRASAGYAFLRVIVPLSLPSLVAGAAMTWARALGEFGATITFAGNLPGRTQTMPLAVY
                     VALQSDLDGAAAMSVLLLLVSFTVLLALRNFPRTVRR"
     gene            772..1845
                     /locus_tag="VEW91_06205"
     CDS             772..1845
                     /locus_tag="VEW91_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91209.1"
                     /translation="MLDAAFSKALGAFELTVEMTAEAGQTLVLVGESGAGKSTILNVL
                     AGLVHPDRGTIRLDATRYFSSEDGIAVPPESRDIGYVFQDYALFPHLSVFENAAFGLR
                     AQGRHPAEVSRRVDGILRQLGVQDLAGRRPSSLSGGQLQRIALARALVLTPRVLLLDE
                     PMSALDLGTRSVVRGELRRVLAALRCITILVTHHPLDAVVFGDRIAVVEGGKFAQVGT
                     REELLRRPRSRYVADLMGLNFFSGRIARREPPGLVEIETGDGTLRIVDPGGEDEIFVA
                     VDPREITLHSGPPAGTAQNVFRGRILELVPEPPLGERIRAVLATRPRLVAEITAHAVR
                     TLGLREGTEVYASFKATAAKGYR"
     gene            complement(1863..2348)
                     /locus_tag="VEW91_06210"
     CDS             complement(1863..2348)
                     /locus_tag="VEW91_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYM91210.1"
                     /translation="MFWAHMNGPWWRGVAGAMLVLLAMLWASVPAHGSQGHAGRPQFS
                     AAVVRTAVALGGRPYQWAGVGPRGFDCSGLVAHVFAALGRPLPHSSFAQFRFGAAIPR
                     WHLVAGDLVFFRTSRPGPSHVGIYIGDGRFVHASYSRGVVVSSIEEPYFNVRFIGGRR
                     I"
     gene            2491..2961
                     /locus_tag="VEW91_06215"
     CDS             2491..2961
                     /locus_tag="VEW91_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HYM91211.1"
                     /translation="MIGMKVRTVAMDQQMNPVVLLVDKSETLALPIWIGTAEAQSIAL
                     ELQGVRMPRPMTHDLIRTMLGQLTVTVNRIVVTDIQNGTYFAEIHLQNNGTDVVVDSR
                     PSDAIALALRMEAPIFVEEKVAAQAIPLKKAFDEHEVEEFKRFLDKVKPQDFRQ"
     gene            complement(2972..3715)
                     /locus_tag="VEW91_06220"
     CDS             complement(2972..3715)
                     /locus_tag="VEW91_06220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HYM91212.1"
                     /translation="MRLRQRVAVITGSSRGIGRAIAQAFAREGAAVVLNAARSIGEAR
                     AAAAAIVSAGGTARAIQADLGNPAEARALVIQAAEELGRIDVLVNNAGIADPADVWQI
                     DEARWARMMAVHVTGPFFAGRAAGEIMIRQGRGVIINIASMRGIEPGTGRLHYAVSKA
                     AVLMLTKCLARDLAPHVRVNAIAPGYTETAFHAERTIAEREQIASRIPLGRFSQPEEI
                     ARAAVFLASDDAAYITGQTLVVSGGVVTG"
     gene            complement(3715..4203)
                     /locus_tag="VEW91_06225"
     CDS             complement(3715..4203)
                     /locus_tag="VEW91_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HYM91213.1"
                     /translation="MEILEVKRTLEGSVEAFPCQAVEATPQRAVLLYTVPRARRIADL
                     DLPAGTMTIAYYWADRPYNVYHWVSPAGQTLGWYFNVSGPIRISEHQVEWQDLEVDVL
                     VTPDLRVQVLDEDRLPGKLGAAQRTAIGAATARVLREYADVAREVEAASCKVLAHLGR
                     ER"
     gene            complement(4312..5469)
                     /locus_tag="VEW91_06230"
     CDS             complement(4312..5469)
                     /locus_tag="VEW91_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HYM91214.1"
                     /translation="MIGEYQAVIRELEGLAHERGLAFDPVVFQITDSDELAQVASMGL
                     PNRFIHWYWGGTYKELVMQQTKEVFSILELVLNTNPSHAFLRSTNSYLQNVLVIAHVL
                     GHADFFANNHWYMKSNKNMLNLAEQHARLIRSYEIQHGRERVEKLLDALLTVATSVNA
                     FERSPQEQRKRLIYYLEERAPLEEFERILLEAILGEAEYFDLIQRTHIINEGWATFVE
                     AELLNRLLSAKEWASISVQLCNRPAPYTIGYTLFAHVRDQDGFDRALELRRFYEDVSL
                     IDVALTEELVRRLDIFVYDAKEKQKSYDLQKVKEMLIAQKLYKGEPRIEVDPASQGRD
                     LLLTHLDEDRKLEPKRVGLFLRAVYSLWRNPVKLRANGKVYTYDRRGLSST"
     gene            complement(5480..6634)
                     /locus_tag="VEW91_06235"
     CDS             complement(5480..6634)
                     /locus_tag="VEW91_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HYM91215.1"
                     /translation="MKIHRGRIAQHKHDHHLREYLKQNLNELIQQKELIIDGKVKTQI
                     ATLDLPTLKFAEEAQYLAQGAGSGGSGAGAGGGAGDERVQALGGLLGGDHHGKELRVE
                     LDFDEFVKLAQEVLLDELELPTFHEPARHGEVDSQDIPELDDLDRIGIRADLNLEETM
                     VQSLFRNARERGVLDYDVDIQQDAWYFVEDPTTFQNNRSVEVYVLDVSGSMRGEYLSL
                     VRKTIFILWHYLERRYPTNLRRYVVFQDVAEEKTRDEFFCVESSGGTHISTGFEKAIE
                     LLEGAREYDKFLFMFTDGETSSGDFDLAKKRFEEARAAFDLVIYCHVNPGGRGIGGFS
                     EYVHETAKAQEGAAFANLMDLETIRAAMKEFLAFFDAQAGRRYTAAPKRQ"
     gene            complement(6651..>7576)
                     /locus_tag="VEW91_06240"
     CDS             complement(6651..>7576)
                     /locus_tag="VEW91_06240"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91216.1"
                     /translation="NFPTVHVDEVVIGHTNYPEYNVFLAEDIMEPLRGRIHKVDFPYN
                     VDVQGEMRIYRALVERANQVRGEAKHIPQDVFELAAAYAIRTRQESQGVRGLSPRFFE
                     DAFSLAYTRAGQCLDLEVMTESIERTFEHQSIKDMNQKDLLKLFEETKVDFVNKKVDQ
                     IVEDIVPTHFYDYGQNLYLNYLDAAARNVLGEQLGDSEKELVDEVEGALVQKRQISRQ
                     GRVAFENVLVERRDELRRMSYRDHEHLRGAINEIVFNKIKNCLRLYEKTEEMDAKSRE
                     LLDVLQRSAVEEHGYCQHCARALFKVIGRSF"
BASE COUNT         1162 a         2641 c         2448 g         1325 t
ORIGIN      
        1 accgcggccc ccgccgcagc aggggcagcg ttcctcgtgc tcctcctcct gccgatgctg
       61 agcctgctcg tccgggtccc acccgctggg ctcctcccca ggctggcgac gccccaggtg
      121 ctccaagcgc tctggttgag cctcgggacg tctttggcag ccaccgtggc ggtcgtcggt
      181 ttgggcctgc ccgtggccta cctgctggcc gtccgcggct ttccggggaa gcgcgtgctg
      241 gaagccctga tcgatcttcc catggtgctc ccccccaccg tggccggggt tgggctgctg
      301 ctggcgttcg gacgggcggg gatcgccggc cgggcgctct cggcgctcgg catcacggta
      361 ccgtttacca cgctcgcggt ggtgctggcc caagcgttcg tggcggggcc cttcttcatc
      421 aacgccgccc gggcgggatt cgaggaagtg gatcccaggt acctgcgggc cgccgcgacc
      481 ctccgggcgt ccgcgggata tgcgttcctg cgcgtgatcg tccccctgag cctgccctcc
      541 ctcgtggctg gggcggcgat gacgtgggcg cgggcgctgg gagagttcgg cgccacgatc
      601 accttcgccg gcaacctccc cggccggacg cagacgatgc cgctggcggt ctacgtcgcg
      661 ctgcagagcg acctcgacgg cgccgcggcg atgtcggtgc tcctcctgct ggtctcgttt
      721 acggtgctcc tggcgctccg caatttcccc cggacggttc gcaggtagac cgtgctggac
      781 gccgcgttct ccaaggccct tggggcgttc gagttgaccg tcgagatgac cgcggaggcc
      841 ggccagaccc tggtcctggt cggcgaaagc ggcgcgggga agtccaccat cctcaacgtc
      901 ctggccgggc tcgtccaccc cgatcgcggg acgatccgcc tcgacgccac ccggtacttc
      961 tcgagcgagg acggcatcgc cgtgccgccg gagtcgcgcg atatcgggta cgtgttccag
     1021 gactacgcgc tctttccgca tctgtcggtc ttcgagaacg ccgcgttcgg gctgcgggcg
     1081 cagggacgcc accccgcgga ggtcagccgc cgcgtggacg ggatcctccg gcaactgggc
     1141 gtgcaggatc tggccgggcg ccgcccttcg agcctgtcgg gaggacagct ccagcgcatc
     1201 gcgctggccc gggccctcgt gctgaccccg cgtgtgctct tgctcgacga acccatgtct
     1261 gcgctcgacc tcgggacccg aagcgtggtg cggggcgaac tccggcgcgt ccttgccgcg
     1321 ctccgctgca ttaccattct cgtgacgcac caccccctcg acgcggtggt cttcggcgac
     1381 cgcatcgcgg tggtcgaagg cgggaagttc gcccaggtcg gcacacggga agaattgctg
     1441 cggcgtccgc gctcccgcta cgtggcggac ctgatgggcc tgaacttctt ctcggggcgg
     1501 atcgcgcgcc gggaaccccc cggcctcgtg gaaatcgaga ccggcgacgg gaccctccgc
     1561 atcgtcgacc cggggggaga ggacgagatc ttcgtggcgg tcgatccccg cgagatcacc
     1621 ctccactccg gcccgccggc cggcaccgcc cagaacgtct ttcgcggacg gatcctcgag
     1681 ttggtgccgg agcctccgct cggagagcgc atccgcgcgg tcctggctac gcggccccgg
     1741 ctggtggcgg agatcaccgc ccatgcggtg cgcacgctgg ggctccggga aggtacggag
     1801 gtctacgcga gcttcaaggc cacggccgcg aagggctacc ggtagctggt gccaccccgg
     1861 cgctagatgc ggcgcccgcc gataaaccgg acgttgaagt agggctcctc gatcgacgag
     1921 acgacgaccc ccctggaata ggacgcgtgc acgaaccggc cgtccccgat gtagatcccc
     1981 acgtgggacg gacccggccg ggaggtccgg aagaacacca gatctcccgc gacaaggtgc
     2041 cacctgggaa tcgccgcccc gaaccggaac tgcgcaaacg acgagtgggg aagcggccgg
     2101 ccgagcgcgg cgaagacgtg cgcgaccagg cccgaacaat caaacccgcg cggccccacc
     2161 ccggcccact ggtacggcct gccgccgagc gccacggccg tccggacgac cgccgccgag
     2221 aactggggcc gtccggcgtg cccctggctg ccgtgggcgg gaacggacgc ccagagcatc
     2281 gcgagcagca cgagcatcgc accggcaacg ccccgccacc acggaccgtt catgtgtgcc
     2341 caaaacactt acgtcctagc cccgcgatct cctgcgcgct ccacgcgcag gtggaacgcg
     2401 ccaccgcgcg ggaaggttgc gtttcgtcca ctccaggact ctgttataat ggacccgcca
     2461 ggaatcgcga gaaccccggg aggcctgagc atgatcggga tgaaggttcg gaccgtcgcg
     2521 atggaccagc agatgaaccc ggtggtgctg ctcgtcgata agtcagagac gctcgcgctc
     2581 ccgatctgga tcggtacggc ggaagcgcag tcgatcgccc tggagctgca gggggttcgg
     2641 atgccgcggc cgatgaccca cgacctcatc cgcaccatgc tcggccagct caccgtcacc
     2701 gtgaaccgca ttgtggtcac ggacattcaa aacgggacct actttgcgga gatccacctg
     2761 cagaacaacg gcacggacgt ggtcgtggac tcgcggccga gcgatgccat cgcgctcgcg
     2821 ctgcggatgg aggcgcccat cttcgtcgag gagaaggtcg ccgcccaggc catcccactc
     2881 aagaaagcgt tcgacgagca cgaggtcgag gagttcaagc ggttcctcga taaggtcaaa
     2941 ccgcaggact tcaggcagta accaaagggc cctaccccgt caccacccca cccgaaacca
     3001 ccagcgtctg gcccgtgata taggccgcgt cgtcggaggc cagaaacacc gccgcgcggg
     3061 cgatctcctc gggctggctg aaccggccca ggggaatccg cgaggcgatc tgctcccgct
     3121 cggcgattgt gcgctcggcg tggaacgccg tctccgtata cccgggggcg atggcgttga
     3181 cccgcacgtg cggcgccaga tcccgcgcca gacatttggt cagcatcagg accgccgcct
     3241 tgctcacggc gtagtgcagc cgcccggttc cgggttcgat cccccgcatc gatgcgatgt
     3301 tgatgatgac gccccggccc tgccggatca tgatctcccc ggcggcgcgg ccggcgaaga
     3361 acgggcccgt gacgtgcacc gccatcatcc gcgcccaccg cgcttcgtcg atctgccaga
     3421 cgtccgcggg gtccgcgatg ccggcgttgt tgaccaggac atcgatgcgc cccaactcct
     3481 cggccgcctg gatcacgagg gcgcgggctt ccgccgggtt ccccagatcg gcctggatcg
     3541 cacgcgccgt gcctcccgcg ctcacgatcg ccgcggcggc ggcgcgggcc tcgccgatgg
     3601 atcgggccgc attgaggacg accgcggcgc cctcgcgggc gaaagcctgg gcgatcgcgc
     3661 gcccgatccc ccgactcgac ccggtgatga cggcgacccg ctggcggagc cgcatcaccg
     3721 ttcccgcccc aagtgcgcca gaaccttgca cgacgctgcc tcgacctccc gcgccacgtc
     3781 cgcgtactcc cgcagcacgc gcgccgtggc ggccccgatc gcggtccgct gggccgcccc
     3841 cagcttcccc gggaggcggt cctcgtcgag gacctggacg cggaggtcgg gggtcaccag
     3901 cacatccacc tcgaggtcct gccactccac ctggtgctcg ctgatgcgga tcgggccgct
     3961 cacgttgaag taccatccca gcgtctggcc ggcgggggag acccagtggt agacgttgta
     4021 cggacggtcg gcccagtagt aggcgatcgt catggtaccc gccggcaggt cgaggtctgc
     4081 gatccggcgc gctctgggga ccgtatacag cagcaccgcg cgttggggcg tcgcctccac
     4141 ggcctggcac ggaaacgcct caaccgagcc ttccagggtc cgcttgacct cgaggatctc
     4201 cacgcgagga ggattcggat cagggcccgc atcccctccc atccatccgg gaaggacgcg
     4261 gcttgcgccc ccgacagggg gggacgagcg ggagattcta cagaacgccg gttaggtgga
     4321 cgacagcccc cggcggtcgt aggtgtacac ttttccgttt gcgcgcaact tcacagggtt
     4381 gcgccacagc gagtacacgg cccggagaaa caggccgacc cgcttcggct ccagcttgcg
     4441 atcttcgtcc aggtgggtga gcaggaggtc gcgtccctgg gaggcggggt cgacctcgat
     4501 ccgcggctcc cccttgtaca gcttctgcgc gatcaacatc tccttgacct tttgaagatc
     4561 atagctcttc tgcttttcct tcgcatcgta gacgaagatg tcgagccggc ggaccaactc
     4621 ttcggtgagc gccacgtcga tcaggctcac atcttcgtaa aaccggcgaa gttcgagtgc
     4681 ccggtcgaac ccgtcctggt cgcgcacgtg ggcgaacagc gtatagccga tggtgtacgg
     4741 cgccggccgg ttgcagagct gcaccgagat gcttgcccac tccttggcgc tgagcagccg
     4801 gttgaggagc tccgcttcga caaacgttgc ccacccctcg ttgatgatgt gggtccgctg
     4861 aatgaggtcg aagtactcgg cctctcccag gatcgcctcg agcaggatgc gctcgaactc
     4921 ttccagcggc gcccgttcct ccaggtagta gatcagccgc ttgcgctgct cctgcgggct
     4981 ccgctcgaac gcgttcaccg acgtcgcgac ggtcagcagg gcgtcgagca gcttctcgac
     5041 ccgctcgcgg ccgtgttgga tctcgtagga tcggatcagc cgggcgtgct gctccgcgag
     5101 gttgagcatg ttcttgttgg acttcatgta ccagtggttg tttgcgaaga agtctgcgtg
     5161 ccccagcacg tgcgcgatca cgagcacgtt ttgcagatac gagttcgtgc ttcgcagaaa
     5221 cgcgtgcgac gggttggtgt tgagcaccag ctcgaggatg ctgaacactt ccttggtctg
     5281 ctgcatcacc agctctttgt aggtgccgcc ccaataccag tggatgaagc ggttgggcag
     5341 ccccatgctg gccacctgcg cgagctcatc gctgtcggtg atctggaaca ccaccgggtc
     5401 gaacgccagc ccacgctcgt gggccaggcc ctcgagctcc cggatgaccg cttgatactc
     5461 gcctatcaag acgctgtcct cattgccgct tcggcgctgc cgtgtatcgc cgcccggcct
     5521 gggcgtcgaa gaacgccagg aactctttca tcgccgcccg gatcgtctcg aggtccatga
     5581 ggttggcgaa ggctgcgccc tcctgcgcct tggcggtctc atggacgtac tcgctgaacc
     5641 cgccgatccc ccgtccgccc ggattgacgt ggcagtagat gaccaggtcg aacgcggcgc
     5701 gcgcctcctc gaaccgcttc ttcgccaggt cgaagtcccc ggagctggtc tcgccgtcgg
     5761 taaacatgaa gagaaacttg tcgtactcgc gcgccccctc caggagttcg atcgccttct
     5821 cgaacccggt gctgatgtgc gtccctccgc tcgactccac gcagaagaac tcgtcgcggg
     5881 tcttctcctc ggcgacgtcc tggaacacca cgtaccgccg caggttggtg gggtaccggc
     5941 gctcgagata gtgccagagg atgaagatcg tcttccggac cagcgagagg tactcgccgc
     6001 gcatcgaccc ggagacgtcc aggacgtata cctccaccga gcggttgttc tgaaaagtgg
     6061 tcggatcctc gacgaagtac caggcatcct gctggatatc gacatcgtag tccaggaccc
     6121 cgcgctcccg ggcgttccgg aacaggctct gcaccatcgt ctcttccaaa ttgaggtccg
     6181 cccggatccc gatccggtcg aggtcgtcca gctcggggat gtcctggctg tccacctcgc
     6241 catggcgcgc gggctcgtgg aaggtcggca gctccagttc gtcgagcagc acctcctgcg
     6301 ccagcttgac gaactcgtcg aaatccagct cgacccgcag ctccttgccg tggtgatcgc
     6361 cgccgaggag cccgcccagc gcctggaccc gctcatcccc ggctcccccg ccggcgccgg
     6421 cgccgctccc gccgctcccc gcgccctggg caaggtactg ggcctcctcg gcaaacttca
     6481 gggtgggcag gtccagcgtg gcgatctggg tcttcacctt cccgtcgatg atgagttcct
     6541 tctgctggat cagctcgttg aggttctgct tgaggtactc ccgcaggtgg tggtcgtgct
     6601 tgtgctgggc gatgcgcccc cggtgaatct tcatcccagg cccccgcggg ctagaagctc
     6661 ctcccgatca ctttgaacag cgcccgggcg cagtgctgac agtagccgtg ctcctccacg
     6721 gcgctgcgct ggagcacgtc gaggagctcg cggctcttgg cgtccatctc ttcggtcttc
     6781 tcgtacaggc gcaggcagtt cttgatcttg ttgaagacga tttcgttgat cgcgccccgc
     6841 aggtgctcgt gatcgcggta actcatccgc cggagctcgt cgcggcgctc caccaagacg
     6901 ttctcgaacg cgacccggcc ctggcggctg atctggcgct tctggaccag cgccccctcc
     6961 acttcgtcga ccagttcctt ctcgctgtcc ccgagctgct cgccgaggac gttgcgcgcc
     7021 gcggcgtcca gatagttcag atataggttc tggccgtagt cgtagaagtg cgtggggacg
     7081 atgtcctcga cgatctggtc gaccttcttg ttgacgaagt cgaccttcgt ctcttcaaac
     7141 agcttcagca gatccttctg gttcatgtcc ttgatcgact ggtgctcaaa cgtccgctcg
     7201 atcgactcgg tcatgacctc caggtcgagg cactggccgg cgcgggtgta ggccagcgag
     7261 aacgcatcct caaagaagcg cggcgacagt ccccgcaccc cctgggactc ctggcgcgtc
     7321 cggatcgcgt aggccgccgc cagctcgaag acgtcctgcg ggatgtgctt ggcctcgccc
     7381 cggacctggt tcgcccgctc gacgagcgcc cggtagatcc gcatctcgcc ctggacgtcg
     7441 acgttgtagg gaaaatccac cttgtggatt cgcccccgga ggggctccat gatgtcctcg
     7501 gcgaggaaga cattgtactc ggggtagttc gtgtggccga tgacgacctc gtcgacatgg
     7561 accgtcggga agttcg
//
LOCUS       DASQGH010000317         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43834, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000317 DASQGH010000000
VERSION     DASQGH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43834"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VEW91_06245"
     CDS             <1..960
                     /locus_tag="VEW91_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM91217.1"
                     /translation="VRAADRLGYATAWVSEAYGWDAFTQLGWLSAVTNRIGLATGIVN
                     VFSRSPALIAQSAATLDRISGGRCILGLGTSGPGVVEGWHGVPFQHALQRLRETVDIV
                     RLVLERKRLAYAGEVFRIQGGIKLAGDPVSGRVPIYLATLGPRALRMTGEIADGWLAA
                     FFSPSQYAAVLGEDLQYGVGRRAADAGPLSICVYHSVVVSADRAVGRDAVRPNLAFYL
                     GAIRSPQHNFYNKLFQRYGFVEEAARVQQLYLDHQHDEAVRAVTDEMVDRVTIIGPPP
                     ECRERLAELASVGVDEVAIQLTVPGGSPADTLAAIQSLAPANA"
     gene            985..>2033
                     /locus_tag="VEW91_06250"
     CDS             985..>2033
                     /locus_tag="VEW91_06250"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HYM91218.1"
                     /translation="MTRPSFPPIYIAGAAEAPLGKVSGHTELSMMAVAAREALAEAGM
                     SLKDVDGLFARYRTNEPMLEHSVELGEYLGIQPRYADSTDIGGGSFEAYVHHAMLAVA
                     TGRCEVALLAYASRQRSRRGRTMQTHGDAYTISGQFEAPYGLVSPIGQYALIAARHMY
                     QYGTTAEQFAEVAVAARRWAQRNPKAWSRDPLTVDDVLASEMICDPLHKLDCCLVTDG
                     GAAVVVTSAARARDAKKPPVRVLGAGESHTHWHIAQMPDLTVTPGVASAREAFAMAGI
                     RHEDVDFIQPYDNFTSAVILHLEDLGFCKKGEGGAFVEHGRLAPGGALPAMTSGGGLS
                     YNHPGMLGLLLLVEAV"
BASE COUNT          336 a          657 c          694 g          346 t
ORIGIN      
        1 gttcgcgccg ccgaccgact cgggtatgcg acggcgtggg tcagcgaagc ctatggatgg
       61 gatgccttca cgcaactcgg gtggttgtca gccgtcacga acaggatcgg actcgcgacc
      121 ggcattgtca acgttttcag ccgcagcccc gcgctcattg cgcaatccgc cgccacactg
      181 gaccgaatca gtgggggacg gtgtatccta gggctcggta cgtccgggcc gggcgtggtc
      241 gaagggtggc atggggtgcc cttccagcac gccttgcaac gcctgcgaga gaccgtggat
      301 atcgtccgcc tcgtgctcga acgtaagcgg ttggcgtatg cgggcgaagt cttcaggatc
      361 cagggcggca tcaagctcgc cggtgatccg gtcagcggcc gcgtgcccat ttacctggcg
      421 acgctaggcc cgcgcgcctt gcggatgacg ggagagatcg cggatggatg gttggccgca
      481 tttttctccc ccagccagta tgcggccgtg ctcggcgaag acctccagta cggggtggga
      541 cggcgcgccg ccgacgcggg gccgctctcg atctgcgtct accactctgt cgtcgtgagt
      601 gcggaccggg ccgtcggccg ggacgccgtg cgccccaacc tggccttcta cctgggcgcg
      661 atacgatcgc cccagcacaa cttttataac aagctctttc aacggtatgg gttcgtcgaa
      721 gaggcagccc gtgtccagca actgtatctt gaccaccagc acgacgaagc ggtgagggcg
      781 gtaaccgacg agatggtcga ccgcgtcacg atcatcggcc cgccgccgga gtgccgcgag
      841 cgcttggcgg aactggcctc ggtgggcgtg gacgaggtcg cgatccagct caccgttcca
      901 ggcgggagcc ctgcggacac gctggccgcc attcagtcgc tcgcgccggc aaacgcatga
      961 ggccgcggcc gagacgatgg taaagtgacg cggccatcgt tcccgcccat ctatatcgcg
     1021 ggggccgcag aggcaccgct cggcaaggtg tccggccaca cagagctctc catgatggcg
     1081 gtggcggcgc gcgaggcgtt ggcggaagcc gggatgtctt tgaaggacgt cgatggcctc
     1141 ttcgcgcgat accgcacgaa cgagccgatg ctcgagcact ccgtcgaatt gggcgagtat
     1201 ctcggcatcc agccgcgcta cgcggattcg accgacatag gcggaggctc gttcgaggcg
     1261 tacgtccacc atgcgatgct cgccgtcgca acgggccgct gcgaggtggc cctgctcgcc
     1321 tatgcctcgc gacagcgcag caggcggggc cggacgatgc aaacccacgg ggacgcgtac
     1381 accatttccg gccaattcga ggcgccgtac gggctcgtat caccgatcgg gcagtatgcc
     1441 ttgatcgccg ctcggcacat gtatcaatac ggaaccacgg cggagcagtt tgccgaggtc
     1501 gcggttgccg cccggcgctg ggcgcagcgc aaccccaagg cgtggtcccg cgatccgctc
     1561 acggtggacg atgtcctggc ctctgagatg atctgtgatc ccctgcacaa gttggattgc
     1621 tgcctcgtga cagatggcgg cgccgccgtg gtggtcacca gtgccgcccg tgcccgcgat
     1681 gcgaagaagc cgccggtgcg ggtcctgggc gcgggcgaga gccatacgca ctggcacatc
     1741 gcccagatgc cggatctcac cgtcacgccg ggcgtcgcgt cagcgcggga agcatttgcg
     1801 atggcaggga tccgccacga ggacgtcgat ttcatccagc cgtacgataa cttcacgagc
     1861 gccgtcatcc tccacctcga ggacctgggc ttctgcaaga agggggaggg aggggcgttt
     1921 gttgagcatg gccgcctcgc tcccggaggg gcgctaccgg ccatgacgtc cggcggcggt
     1981 ctctcctaca accaccccgg gatgctgggg ttactcctgc tcgtggaagc cgt
//
LOCUS       DASQGH010000318         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24093, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000318 DASQGH010000000
VERSION     DASQGH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24093"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VEW91_06255"
     CDS             <1..413
                     /locus_tag="VEW91_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYM91219.1"
                     /translation="KRAPLPLFGDRDAGEPNLLALNIQPDEAISLRFVAKAPGTTMSL
                     RPVDMGFRFGTAFGTEEMSAYERLLLDCLLGDTTLFTRRDEVEEAWDLVEPILRWWEH
                     STPPEPPPTYAAGTWGPEAARTLIAGNNRRWRQL"
     gene            446..1255
                     /gene="pgl"
                     /locus_tag="VEW91_06260"
     CDS             446..1255
                     /gene="pgl"
                     /locus_tag="VEW91_06260"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HYM91220.1"
                     /translation="MQPGASPELVVLPDLASAAAVAAERFVSRCARAVADRGECAIAL
                     AGGDTPRALYARLAADPVRGRIAWGQLRLFWGDERGVSPQDARSNFRMAQEALLSRVP
                     VPDDRIHRMPAERPDLEEAAEAYAALLREHLPAAAEGWPRFDLVLLGLGEDAHTASLF
                     PGAPALRETRRAVVAYEVPHLGMSRMTLTLPVLNHAGEVLFLVSGAQKAQALWAVLEG
                     SRDPDRVPAQGIRLAGGRVVWVVDAAAASRLTVKPGPRGGGFSVSPAESPR"
     gene            1297..>1542
                     /locus_tag="VEW91_06265"
     CDS             1297..>1542
                     /locus_tag="VEW91_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91221.1"
                     /translation="MYRVWRWGALSLILLLAAGVPLGRPVPAGAAAEQLTIGHSTWVG
                     YGPLFLARDKRFFDEAGVAVELPTIEDPKLRFAALAAG"
BASE COUNT          194 a          542 c          573 g          233 t
ORIGIN      
        1 tcaagcgggc cccgctgccg ctcttcgggg accgcgatgc gggggagccc aacctgctgg
       61 cattgaacat ccagcccgac gaagccatct cgctcaggtt cgtggcgaag gcgcccggca
      121 caacgatgtc cctccggccg gtggacatgg ggttccggtt cgggaccgca ttcggcaccg
      181 aagagatgtc cgcctacgag cggctgctgc tcgactgctt gctcggcgac accacgctct
      241 tcacccggcg ggatgaggtc gaggaggcct gggacctggt ggagccgatt ctgcgctggt
      301 gggagcactc cacgccgccc gagccacccc cgacctatgc cgcaggaacg tggggccctg
      361 aagccgcccg tacgctgatc gccggcaaca accgccgctg gcggcagttg tgacccagcc
      421 cgggagggcg tcgcggcggg agccggtgca gccgggcgca tcccccgaac tggtcgtcct
      481 gccggacctc gcctcagccg ctgcggtggc ggcggaacgg ttcgtcagcc ggtgcgcgcg
      541 cgcggtcgcc gatcgcggcg agtgcgcgat cgcgctggcg ggaggcgaca cccccagggc
      601 cctgtacgca cgcctcgccg ccgatccggt tcggggccgg atcgcctggg gccaacttcg
      661 gctcttctgg ggagacgagc gtggggtctc cccccaggat gcccgcagca acttccggat
      721 ggcgcaggag gcgctgctgt cgcgcgttcc cgttcccgac gaccgcatcc accggatgcc
      781 ggccgaacgc cccgacctcg aggaggccgc cgaggcgtac gccgcgctgc tccgcgagca
      841 cctgccggcc gctgcggagg gctggccgcg gttcgacctc gtcctcctcg gactggggga
      901 ggacgcccac accgcatcgc tcttcccggg cgcgccggcg cttcgggaga cgcgccgggc
      961 cgtggtggcc tacgaggttc cccaccttgg gatgtcgcgc atgaccctca cgctgccggt
     1021 cctcaaccac gccggtgaag tgctgttcct cgtctccggg gcgcagaagg cccaggcgct
     1081 gtgggcggtg ctcgagggct cccgggaccc cgaccgcgtg ccggcgcagg ggatcaggct
     1141 ggccggcggg cgggtggtct gggttgttga cgcggccgcg gcctcgcgcc tgacggtcaa
     1201 gcccggccca agggggggag ggttttccgt aagcccggcg gaatcccccc ggtgagccta
     1261 ccagcgggcc taatcaggaa gggagggggt ttgcgtatgt atcgcgtctg gaggtggggg
     1321 gctttatccc tcatcctgct gctggccgcg ggagtgcccc tcgggcggcc tgtgccggcc
     1381 ggggcggcgg cggagcagtt gaccatcggg cactcgacct gggtcgggta tgggccgctg
     1441 ttcctggccc gcgacaagcg cttcttcgac gaagcggggg tcgcggtcga gctccccact
     1501 atcgaggatc cgaagttgcg gttcgcggcc ctcgccgcgg gc
//
LOCUS       DASQGH010000319         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32598, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000319 DASQGH010000000
VERSION     DASQGH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32598"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..2805
                     /gene="ligD"
                     /locus_tag="VEW91_06270"
     CDS             160..2805
                     /gene="ligD"
                     /locus_tag="VEW91_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYM91222.1"
                     /translation="MGLEEYQRKRRFASTPEPQGTFGPGAERPRFVVQEHHARRVHWD
                     LRLEMEGVLRSWAVPKGPSLDPADKRLAVLVEDHPIEYGEFEGIIPEGNYGAGTVMLW
                     DRGTYECVEGDPAEAFRRGKLTLIFHGEKLRGEFHFVRTKRNEGRDWLLFKGKDEFAA
                     PGYTATGTRSVKTGRTIEEVRAEQDARWTASAPAAAAPAPSRPPSPRRALRKAPADPF
                     PEPFRPMLAQTAEGPFSRAGWLFEIKWDGVRALGFVRRRGATQEITLYSRTLRRLNAQ
                     FPEVIEALAALAPLAGDGAVLDGEIFAPDERGRPNFARLQQRVHLSREPDVREAAGRI
                     PVVFAVFDCLYLNGRDLRAHPLSERRTMLEGLALPAGMLRSDAVEGDGLTLFEAATQH
                     GLEGIIAKRASSLYRPGARSADWQKIKVRRTLDAVVGGITRGKGSRKGTFGALVLGQY
                     DPSTGALVHIGQTGGGLSDSDLRVLRRRLEPLVTKTCPFVVPPTPLQPVTWVRPSVVV
                     EVEYSEFTPDRMLRAPVFLRVRDDIPAAEVKRRDGDGTTPAAAPPVAFTNLDKVFFPD
                     LGLTKGDVIGYYRRIAPYLLPHLRDRPLTLRRWPDGIAGESFFQKDLEDPPAFVRTER
                     IWSADGHRDVRMVICNDEATLLWLAQLGCIEMHAWFSRITPIPGRRDRPGAGFAGSEE
                     AIAKSTLNVPDYVVFDIDPFLFPEGTGPTRRHGEYDPDYTRKGFEAARQAALWLGEAL
                     EGLGLRAFAKTSGKTGLHVFIPIVRRYTYAQTHAFAKTMATWLVRQHPGALTTAWSVR
                     DRVGKVFLDYNQNVRGKTLASIYSLRPVPQATAGVPVTWDELRAGLDPLRWTITTVFD
                     RLERVGDLWADILNAAQEIAERARG"
     gene            2966..>3275
                     /locus_tag="VEW91_06275"
     CDS             2966..>3275
                     /locus_tag="VEW91_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HYM91223.1"
                     /translation="MPAHPAFVWFDGQVVPWDQAKVHVATATVLRGANVFEGVRAYWN
                     AGERELFIFRNDAHMARLWNSAKIMRMTIPWTKEELTRAQMEVLRKNAFEDTVWFRLT
                     L"
BASE COUNT          510 a         1067 c         1180 g          518 t
ORIGIN      
        1 ccaaatcgag cgcccccctc tccgtgacct ggtaggcgat attgacgtcg ccgctcttgg
       61 cgtacctggt ttccggacga ttgggccctg tcatcccggc acgcaccatt ggcatcgcgg
      121 ccgatcgcgt gggggtcggg cgtttgatac gatcatcccg tgggactgga ggaatatcag
      181 cggaagcgtc gctttgcgtc caccccggag ccgcagggga ccttcggccc cggagcggag
      241 cgaccgcgct tcgttgttca ggagcaccat gcgaggcgtg tgcactggga cctccgtctg
      301 gagatggaag gcgttctgcg ctcgtgggcc gttcccaagg gaccatcgct ggatccggcg
      361 gacaagcgtc tggcggtgct cgttgaggac caccctatcg aatacgggga gttcgagggc
      421 atcattcccg agggcaacta cggcgccggg acggtcatgc tgtgggaccg cggcacctac
      481 gaatgcgtgg agggcgaccc cgccgaggcg ttccggcgcg ggaagctgac gctgatcttt
      541 cacggcgaga agctccgggg cgagttccac tttgtccgca ccaagcggaa cgagggccgc
      601 gactggctct tgttcaaggg caaggatgaa ttcgcggcgc cggggtacac ggcgaccggg
      661 acacgctcgg tgaaaaccgg ccgcacgatc gaggaggtcc gcgccgagca ggatgcccgc
      721 tggaccgcgt cggcccctgc cgcagccgcc cccgcgccgt cgcggcctcc gtcgccgcgc
      781 cgtgccctgc ggaaggcgcc ggccgatccc tttcccgagc cgttccggcc gatgctcgcg
      841 cagaccgccg agggtccctt tagccgcgcc gggtggctgt tcgagatcaa gtgggacggc
      901 gtccgcgcgc tgggcttcgt gcgccgccgg ggagcgaccc aggagatcac cctgtacagc
      961 cgcacgctcc gccggctgaa cgcccagttc cccgaggtga tcgaggcgct cgccgccctc
     1021 gctccgctcg ccggggacgg cgccgtgctc gacggcgaga tcttcgcgcc ggacgagcgc
     1081 gggcggccga acttcgcgcg tctgcagcag cgggtgcacc tgagccggga gcccgatgtc
     1141 cgggaggcgg ccgggcggat cccggtggtg tttgcggtgt tcgactgcct gtacctcaac
     1201 gggcgggacc tgcgggccca tccgctctcg gagcggcgca cgatgctcga aggactcgcg
     1261 ttaccggccg ggatgctgcg gagcgacgcc gtggagggcg acggcctgac gctctttgag
     1321 gccgccaccc agcacgggtt ggagggaatc atcgcgaaga gggcatccag cctctaccgg
     1381 cccggcgcga gaagcgcgga ctggcagaag atcaaggttc ggcggactct ggacgcggtg
     1441 gtggggggca tcacgcgcgg gaagggttcc cggaaaggaa ccttcggggc gcttgtgctc
     1501 ggccagtacg atccgagcac cggtgcgctc gtgcacatcg gccagacggg cggggggctg
     1561 agcgatagtg accttcgggt gctccgcagg cggctcgagc ccttggtgac caagacgtgc
     1621 ccgtttgttg ttcctcccac cccgctgcag ccggtcacgt gggtgcggcc cagcgtcgtc
     1681 gtggaggtgg aatactcgga gttcacgccc gaccgcatgc tgcgcgctcc cgtgttcctg
     1741 cgcgtgaggg acgacatccc ggccgcggaa gtgaagcggc gcgacggcga tggcaccact
     1801 cccgcggcgg cgccgcctgt ggcgttcacc aacctcgaca aggtcttctt tccggacctg
     1861 gggctgacga agggggatgt gatcggctac tatcgccgga tcgcccccta cctcctcccg
     1921 cacctccggg atcgcccgct cacgctgcgg cggtggcccg acgggatcgc cggcgagtca
     1981 ttttttcaaa aggatctgga agacccgccc gcgttcgtga ggacggagcg catctggtcg
     2041 gcggacgggc atcgggacgt ccgcatggta atctgcaacg atgaggccac gctcctctgg
     2101 ctggcgcagc ttggatgcat cgagatgcac gcctggttct cccggatcac gccgatcccc
     2161 ggccgccgcg accggcccgg ggccgggttt gcgggctcgg aggaggcgat cgcgaaaagc
     2221 acgctcaatg tccccgacta cgtggtgttc gacatcgacc catttctgtt tcctgaaggc
     2281 acggggccga ccaggcgcca cggcgagtac gatcccgact acacccgcaa agggttcgag
     2341 gctgcgcggc aggcggccct gtggctcggc gaggcgctcg agggcctcgg gctccgcgcg
     2401 ttcgccaaga cctcgggaaa gacgggcctg cacgtcttca tcccgatcgt ccgccggtac
     2461 acctacgcgc agacgcacgc gttcgccaag acgatggcca cgtggctcgt gcgccagcat
     2521 cccggggcgc tgaccaccgc gtggtcggtg cgggaccgcg tgggcaaggt cttcctggac
     2581 tacaatcaga acgtgcgcgg caagacgctg gccagcatct acagcctccg gccggtcccg
     2641 caggcgacgg cgggcgtccc ggtcacgtgg gacgagttgc gggccgggct ggacccgctc
     2701 cggtggacga tcacgacggt gttcgaccgg ctggagcgcg tgggggacct gtgggcggac
     2761 atcctcaacg ctgcgcagga gatcgccgag agggctcggg gatgaggggc ttcgtcgccg
     2821 gcggcacacg gtccgggtgg gcgccggccg tcaagagcgt tgcggagggc tggccgctgg
     2881 tcgcggcagc gttggccgac cccttgaccg gatgaggagt gggcacatag aatcaaccgg
     2941 gaaccgcata ggaggcgcat gaggcatgcc ggcacatcca gcgttcgtgt ggttcgatgg
     3001 tcaggtcgtc ccctgggatc aggcgaaggt gcacgttgcg actgcaaccg tgctgagagg
     3061 cgcgaacgtc ttcgaagggg tccgtgccta ctggaacgca ggggagcgcg agctcttcat
     3121 cttccgaaac gacgcgcaca tggcgcgcct ctggaactcc gcgaagatca tgcggatgac
     3181 gatcccgtgg accaaggagg aactcacccg ggcccagatg gaggtgctgc ggaaaaacgc
     3241 ctttgaggac acggtgtggt tccgcctcac actgt
//
LOCUS       DASQGH010000320         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_40991, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000320 DASQGH010000000
VERSION     DASQGH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40991"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VEW91_06280"
     CDS             <1..369
                     /locus_tag="VEW91_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91224.1"
                     /translation="RLLAECGLGAVRRQPGWTLSGGERRRVEIARALSLEPTFLLLDE
                     PFTGIDPRSVQELQRTIRYLRERGLGLVITDHNVRDTLAITDRVEIIHEGRILLSGKP
                     EELLESAEARRLYLGEGFHL"
     gene            366..1451
                     /locus_tag="VEW91_06285"
     CDS             366..1451
                     /locus_tag="VEW91_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HYM91225.1"
                     /translation="MRLSLLDRLFCTEIGISFGFGLGFFTTLLVMNHVFYLARLAISQ
                     GMPFGIALQLFVYKVPYLVAFSAPMGVLLATVLGIGRMTDHNEIAALRVCGTSLYRIA
                     APVVLLGCVAAVGIVVFAEGVVSIANDRYRVVFSDFMTKAPELQPVENVFFQGPGAEG
                     NALYYAQRYNPSTQTLEGVTVIYLTGGQALRIIQAHRATYRQGGAWTFHDGEVYVAEQ
                     GRVVTTRFGAMDLNVPRTPQDLTLPPKPLVDMSLRELSGEISAARRRGADPRPFVLET
                     HNRLAAAASSIVFALVGVPLSLRPHRSGPSIGMGLSILVLFAYYAVLIPSQLASEGRV
                     LPPALAAWLPNGVVGALGGALLAKAAR"
     gene            1448..2347
                     /locus_tag="VEW91_06290"
     CDS             1448..2347
                     /locus_tag="VEW91_06290"
                     /inference="COORDINATES: protein motif:HMM:NF022727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HYM91226.1"
                     /translation="MNVAVILIPTLVLVSGLVAFVGNLVGRGIGRRRLALGGLRPRHT
                     AQLITVLTGMFIASVTLAVVLLVSEDARQALFHLQEVRQQIAQQEAQLRALQVRDILY
                     LRDQEVLRTVVDGREPTAVVRNRVQAFFDLAVRSAQERGVAPGPDGVVVRMSPSGLTV
                     DDVAQDIVERHQPMVVRMIATANTVRGEPLEATVIGFPNTLVFTAGEAIVTRRLDGRS
                     DRAGIEAGLLDLSAEVAATAKQRGVISPAFALASSPPDVRLDPAVLLATLERVQASRA
                     PVEVQGAVTSDTYTIGPVVVTFR"
     gene            2344..2754
                     /locus_tag="VEW91_06295"
     CDS             2344..2754
                     /locus_tag="VEW91_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-16S rRNA-processing nuclease YqgF"
                     /protein_id="HYM91227.1"
                     /translation="MRVLAVDPGTEKCGVAVSGPEGVVASRVVGISDLPALVRDWVET
                     YGVRVVVVGNQTGSKRVFDIMKGLPVKVEGVAERDSTMLARRRYFADHPPRGWKRLIP
                     RSFQIPPEPYDDYAAVVLAEAYLAGAGESGGKKP"
BASE COUNT          411 a          976 c         1031 g          472 t
ORIGIN      
        1 cgcctcctgg cggagtgcgg cctcggggcg gtgcgccggc agccggggtg gacgctgtcc
       61 ggaggggaac ggcgccgggt cgagatcgcc cgggccctct ccctcgagcc gacctttctg
      121 ctgctcgacg agccgttcac cgggatcgat ccgcggtcgg tgcaggagct ccagcggacg
      181 atccgctacc tgcgcgagcg ggggttgggg ttggtgatca ccgaccacaa cgtccgcgac
      241 acgctcgcca tcaccgatcg cgtcgagatc atccacgaag gccggatcct gttgtccggc
      301 aaacccgaag aactcctcga gagcgcggag gcccgccggt tgtacctcgg cgaggggttc
      361 cacctgtgag gctctcgctg ctcgaccggc tgttctgcac ggagatcggc atctcgtttg
      421 gctttgggct ggggttcttc accaccctgc tcgtgatgaa ccacgtcttc tatctggcgc
      481 ggctggccat cagccagggg atgccgttcg ggatcgcgct gcagctcttt gtgtacaagg
      541 tgccgtacct ggtcgcgttc agcgcgccga tgggcgtgtt gctggccacc gtgctcggaa
      601 tcggccggat gaccgaccac aacgagatcg ccgcgctccg ggtctgcggg acgagcctgt
      661 accggatcgc cgcccctgtc gtcctcttgg gatgcgtcgc cgccgtgggg atcgtggtgt
      721 tcgccgaagg ggtcgtcagc atcgcgaacg accgctaccg ggtggtgttc agcgacttca
      781 tgaccaaggc gcccgagctc cagcccgtcg agaacgtctt ctttcagggc ccgggcgcgg
      841 aggggaacgc gctgtactac gcgcagcgct acaacccgtc cacccaaacc ctcgaggggg
      901 tgacggtgat ctatctcacc ggggggcagg cgctccggat cattcaggcg caccgggcga
      961 cgtaccggca gggaggcgcg tggacgtttc atgacggtga ggtgtacgtc gccgagcagg
     1021 ggcgcgtggt gaccacccga ttcggcgcga tggacctgaa cgtgccgcgc accccccagg
     1081 acctcacgct gccccccaag ccgctggtgg acatgagcct gcgcgagttg tccggggaga
     1141 tttcggcggc ccggcgccgg ggcgcggacc cccgcccctt cgtcctggag acgcacaacc
     1201 ggctggccgc ggcggccagc agcatcgtgt tcgcgctcgt cggggttccg ctcagcctcc
     1261 gcccccaccg atcggggccc agcattggta tggggctgag catcctcgtc ctgttcgcct
     1321 actacgcggt cctcatccca tcccagctgg cctccgaggg gcgcgtgctg ccccccgccc
     1381 ttgccgcctg gctcccgaac ggggtcgtcg gcgcgctcgg cggggcgctc ctcgccaaag
     1441 cggcccggtg aacgtcgccg tcatcctgat cccgacgctg gtcctggtca gcgggctggt
     1501 cgccttcgtc gggaacctgg tcgggcgcgg catcggccgc cggcggctgg cgctgggcgg
     1561 cctccgtccc cggcacaccg cgcagctgat cacggtcctg accggcatgt tcatcgcctc
     1621 agtcaccctc gccgtcgtgc tcctggtgag cgaggatgcg cgtcaggcgc tgttccacct
     1681 ccaggaggtg cgccagcaga tcgcccagca ggaggcccag ctgcgcgcgc tccaggtgcg
     1741 ggatatcctg tacctgcgcg atcaggaggt cctccgcacc gtcgtcgacg gccgcgagcc
     1801 gaccgccgtc gtccgcaaca gagtccaggc cttcttcgat ctcgcggtgc ggtcggccca
     1861 ggagcgcggc gtggcccccg ggcccgacgg ggtggtggtg cggatgtccc cctcgggcct
     1921 cacggtcgat gacgtggccc aggacatcgt cgagcgccac cagccgatgg tggtacggat
     1981 gatcgccacc gccaacaccg tgcgggggga accgctggag gcgacggtca tcgggtttcc
     2041 caacaccctg gtcttcacgg cgggggaggc gatcgtcacc cggcggctcg acgggagaag
     2101 cgaccgcgcg gggatcgagg cggggctcct cgacctctcg gccgaggtgg ccgcgaccgc
     2161 gaagcagcgc ggggtcatct cccctgcatt cgcgctcgcg tcgagccccc ccgacgtccg
     2221 gctcgatccc gcggtgctcc tggcgacgct ggagcgggtg caggccagcc gcgctccggt
     2281 cgaggtccag ggagcggtga cgtccgacac gtatacgatc ggaccggtcg tcgtgacgtt
     2341 caggtgaggg tgctggccgt ggatcccgga acagagaagt gcggggtcgc ggtgtccggt
     2401 cccgagggtg tcgtggccag ccgggtggtc gggatctcgg atctccccgc ccttgtccgg
     2461 gactgggtgg agacctacgg ggttcgggtt gtggtggtag gaaatcaaac aggatccaaa
     2521 cgtgtgttcg acattatgaa gggcctgccc gtcaaggtgg agggcgtggc cgagcgggac
     2581 tcgaccatgc tggcgcgccg gagatacttt gcggatcacc ccccgagggg ctggaaaagg
     2641 ctgattccga ggtccttcca gatccccccg gagccgtacg acgactacgc ggcggtggtc
     2701 ctggccgagg cctacctggc cggggcgggg gaatccggcg ggaagaagcc ttgacactgc
     2761 ctgaaaaatg ccggtaaaat gacaccggat cgccctggtg agcatacggg cgcaaggcgt
     2821 cgcaccctgg cggggaggct cccaggaggc ggcacgcgca gttgtccggc ccaccaggcg
     2881 taaagaccgg
//
LOCUS       DASQGH010000321         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_43844, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000321 DASQGH010000000
VERSION     DASQGH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43844"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1236)
                     /locus_tag="VEW91_06300"
     CDS             complement(106..1236)
                     /locus_tag="VEW91_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91228.1"
                     /translation="MAHDLVAARQAEQRQREAARARTPLQLAWRQLTRYKLALASGVV
                     LVVAYVVMLFAEFFAPYALDFTDRTVFYAPPVGVHLRDAQGRWHPRPFVYAYRLVDRD
                     LRIYGPDPSRTYELRFFVRGFRYRLLGFVPTDVHLLGVDPPGRLFLLGTDQFGRDLLS
                     RMLFGSRISLLIGVLVVLITFPIGLVLGGVAGFYGKLVDNIIMRSVEVLASFPTFYLL
                     LTLASVLPATLSSGGRLFMISAVFSLVGWGGLARIIRGLVLSIRELEFVLAAQASGLS
                     DLRVIVRHILPSTSSYVIVAATLSIPGTILGESGLSFLGLGVQEPSTSWGLLLAQAQS
                     IDVLTHFPWLLLPGLAIVVVILAYNLLGDGVRDALDPRQRSS"
     gene            complement(1244..>2192)
                     /locus_tag="VEW91_06305"
     CDS             complement(1244..>2192)
                     /locus_tag="VEW91_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91229.1"
                     /translation="LLLGPLLIGITFVSWAAIQLAPGSGDYFQSLVLQYPQISPQTIA
                     ALRARFGMDQPPWIQYLRWLWSIVHLDFGLSFAYQVPVTWLIGSRALNTLLLSMTSMA
                     AAWAIAIPLGIYSAVHQYSVMDGVANVSAFIAISVPSFFSALLLLYAAFWTHLLPLQG
                     LTSVNYDSLSWGRKVLDVGWHLILPTVALGVFSVGGLMRYMRNNLLDILRADYLKTAR
                     AKGLAERRVIFRHAVRNAINPLVTLFGFELGGLLSGAAFVENVLGYPGLGRLILEAVL
                     KKDVFVVMGSLLLGSVLLILGNLTADILLAYVDPRIRYD"
BASE COUNT          422 a          718 c          798 g          254 t
ORIGIN      
        1 gccctgctgg acctgggcga tccggtacca gaggccgccc ccgccggcga ccaccacggc
       61 cagggcgagg atgcccagca ccacggtgcg gccgcggagg cgcatctagg aggaccgctg
      121 ccgggggtcg agggcgtccc ggaccccgtc gccgaggagg ttgtaggcga ggatcaccac
      181 gacgatcgcc agcccgggga ggagcagcca cggaaagtgg gtgagcacgt cgatgctctg
      241 ggcctgggcc aggaggagcc cccagctggt gctcggctcc tggacgccca acccgagaaa
      301 cgacagcccg ctctccccca ggatcgtccc cgggatcgac agcgtcgcgg cgacgatcac
      361 gtacgacgag gtgctcggca ggatgtggcg gacgatgacc cggaggtcgc tcagcccgga
      421 cgcctgggcg gccaggacga actccagctc ccggatgctg agcaccagcc cccggatgat
      481 ccgggccagc ccgccccacc ccaccaggct gaagaccgcg gagatcatga acaggcgccc
      541 cccgctgctg agggtggccg gcagcaccga ggcgagggtg agcagcaggt agaacgtggg
      601 gaacgacgcc aggacctcga ccgaccgcat gatgatgttg tcgacgagct tcccgtagaa
      661 cccggccacg ccgcccagga ccagcccgat ggggaacgtg atcaggacga ccagcacccc
      721 gatcaggagc gagatgcggc tgccgaacag catgcgggag aggaggtccc ggccaaactg
      781 gtcggtcccg aggagaaaca gccgccccgg agggtccacc cccagcaggt ggacgtcggt
      841 cgggacgaac ccgagcagcc ggtaccggaa cccgcggacg aagaagcgga gctcgtaggt
      901 gcgggatggg tccggcccat agatccggag gtcgcgatcc acgagccggt aggcgtagac
      961 gaagggccgg gggtgccagc ggccctgggc gtcccggagg tgcaccccca cggggggcgc
     1021 gtagaacacg gtccggtcgg taaagtcgag cgcgtacggg gcgaagaact cggcgaacag
     1081 catgacgacg tacgccacga ccaggaccac gccgctcgcg agcgcgagct tgtagcgggt
     1141 gagctggcgc caggcgagct gcaggggcgt gcgggcgcgc gcggcctccc gctgccgctg
     1201 ctcggcctga cgcgctgcga ccagatcgtg cgccactggg ccctcagtcg tagcggatcc
     1261 gcgggtcgac gtaggcgagc aggatgtccg cggtgaggtt gccgaggatc agcagcacgc
     1321 tgcccaacag caggctcccc atcacgacga acacgtcctt cttgagcacg gcctcgagaa
     1381 tcagccggcc gagcccgggg tagccgagga cgttttccac aaacgccgcc ccgctgagca
     1441 acccgcccag ctcgaacccg aacagggtca ccagcgggtt gatggcgttg cggaccgcgt
     1501 gccggaagat cacccgccgc tcggcgaggc ccttggcgcg cgcggtcttc aggtagtcgg
     1561 cgcgcaggat gtccagcagg ttgttgcgca tgtaccgcat cagcccgccg accgaaaaca
     1621 cgccgagcgc gaccgtcggc aggatgaggt gccagccgac gtcgagcacc ttcctccccc
     1681 aggagaggct gtcgtagttg acgctggtga gcccctgcag ggggagcagg tgggtccaga
     1741 acgcggcgta gagcagcagg agggcgctga agaagctcgg gaccgagatc gcgatgaagg
     1801 cggagacgtt ggccacgccg tccatcaccg aatactggtg gaccgcggaa tagatcccga
     1861 gcgggatggc gatcgcccac gcggccgcca tcgacgtcat cgacagcagc agggtgttga
     1921 gcgcccggct cccgatgagc caagtgacag gaacctggta cgcaaacgag aggccaaagt
     1981 ccaggtggac gatgctccac agccagcgga ggtactggat ccacggaggc tggtccatcc
     2041 cgaaccgggc gcgcagggcc gcaatggtct gggggctgat ctgcgggtac tgcaggacga
     2101 gcgattggaa gtaatcgccc gacccgggcg ccagctggat cgccgcccag ctgacgaagg
     2161 tgatcccgat gaggaggggc cccaggagga gc
//
LOCUS       DASQGH010000322         1989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24132, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000322 DASQGH010000000
VERSION     DASQGH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24132"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1262..>1989
                     /locus_tag="VEW91_06310"
     CDS             1262..>1989
                     /locus_tag="VEW91_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYM91230.1"
                     /translation="MLDLTRLLPGGYATMLLADLGAEVIKIEAPGAGDWTREPSPIFG
                     GTNALFAQINRNKKSLVLDLKAEEGREVLLRLAARSEALVEGFRPGVAARLGIDYGAV
                     RKRSPRIVYCSLSGYGQTGPNRDRPGHDLNYVGEAGLLGLDDGQEPRVPPLPFSDLAG
                     AIFTALTLAAGMKGAAETGAGAYIDLGMSDTIVSLLTVHFAQMFQAGRPPSLADAYLL
                     GGHPCYTVYRASDGKHLTVGALEP"
BASE COUNT          321 a          653 c          670 g          345 t
ORIGIN      
        1 atcccgctcc ccattcgaga cgtcggtaga ttcacgacgt tcgcgtcggc aagagtgcaa
       61 gccgcgtagc ggcgatatct gggttagccc cgggcggaag cccggggtaa gcggcaacaa
      121 gagaatccaa gccccttcca ggggcgacgc ctggcccttg tgtgccgccc cttgaagggg
      181 ggctctgtca tctcttgcaa tgcgtacccg gcgctgacgc gccgggctaa cccaaatgtc
      241 gccctccggc ccttcgactt cgctcagggc tcggcctcgc attgctcggc ctcggctttg
      301 gcgaagcggg gtcgcgacct gggcgggcga ggggcggtcg cctccacgca agccttggac
      361 gtagctcggc caggtacagg cccggagggc cttgcccaaa cgtagcccag cgcttctagc
      421 gctgggtaag gttgtccatg aacccagtcc cggagggacg ggcctcgtgt cctatgtgtt
      481 agcctcggcg catgtctcac acctacacgg ctaaccgtct gcacgtggtc ttcagcacca
      541 aacagcgccg ggacctgatc tcgcaggaac tccagccgcg cctttgggct tacatggctg
      601 agattggacg gaatcgggcc atccgcattc tcgccgttgg tggcgtcgcg aatcacgcgc
      661 acgtgctgct ctcgttgcct cccgtgatgc ccctggcgaa agcggcccag acgctgaaag
      721 gcatctcctc gaaatggatg aaccaaatag gacgcggcgg ctttgcatgg caggaagggt
      781 atgcggcctt cagtccccta gacccagcgt tgaaacgctg ggctacgttg ggcaaggccc
      841 tccgggcctg atgccgtggt ggaacgagcg gagcggcgac atttgggtta gccccgggtg
      901 gaagccccgg ggtcaggagc aacaatagaa agcccctctt agggcgacac ctcgccggtg
      961 ccgtccctaa gggactcgga agacatttgc catgctgacc cggcgctcaa gcaccccagc
     1021 gaaccaaaag cgggttcgct ggggaccccg ctgacgcgcc gggctaacac aaatgtcgcc
     1081 cttcggccct tcgacttcgc tcagggctcg gcctcgcatt gctcggcctc ggctttggcg
     1141 ggactgggtc gcgcccagac ggccgggggc agtcggcttc gctcgggtag gctcaccacg
     1201 tgattctggg cgaaacgggt ttatcatcgc gggcatgtgg aacctgctgc agggagcgcg
     1261 cgtgctcgac ctgacgcgtc tgttgccggg cggctatgcc accatgctgc tggcggacct
     1321 gggcgcggag gtcatcaaga tcgaagcgcc cggcgcgggc gactggacgc gcgagccttc
     1381 gcccatcttc ggcggcacca acgccctgtt cgcgcagatc aaccgcaaca agaagagcct
     1441 ggtgctcgac ctgaaggcgg aggaaggccg tgaggtcctg ctgcggctgg cggcgcggag
     1501 cgaggcgctc gtcgagggat tccgtcccgg agtcgcggcg cggctgggca tcgactatgg
     1561 agcggtgcgc aagcgcagtc cgcggatcgt ctattgctcg ctgagcggct acggccagac
     1621 cggtcccaac cgcgaccgcc ccggccacga ccttaattat gtcggcgagg cgggactgct
     1681 cgggctcgac gacgggcagg agccgcgggt gccgccgctg ccgttttccg acctggccgg
     1741 cgccatcttt accgcgctca ctctggccgc cgggatgaag ggcgcggccg agaccggggc
     1801 cggggcgtat atcgacctcg gcatgtcgga caccatcgtc tcgctgctca ccgtgcactt
     1861 cgcgcagatg ttccaggcgg gccggccgcc ctcgctggcc gatgcctatc tgctcggtgg
     1921 gcatccttgt tatacggttt accgcgcctc tgacggcaag cacctgaccg tgggcgcgct
     1981 ggagccgaa
//
LOCUS       DASQGH010000323         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49538, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000323 DASQGH010000000
VERSION     DASQGH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49538"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1160
                     /locus_tag="VEW91_06315"
     CDS             141..1160
                     /locus_tag="VEW91_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYM91231.1"
                     /translation="MRAALFHGPGKPLTVQEVETPKIGPGEALVKVAACGVCATDLHY
                     LHGTPTFKKPPLILGHEVSGRIEAVAPDIKGLAPGESVLVPAVLSCGECLNCRRGRDN
                     LCERMVMPGNHADGGFAEFLKVPARTLVPLPPGLPLLESAVISDAVSTPFHAVRNRGH
                     VQGGDWVAIFGCGGVGINAVQIAAALGATVIAVDVDPKKLAQARELGAAEALNAKEGD
                     VPKTIRKLTGGGVDVAFEIVGKPQVLDQALGSVRAGGTLVAVGYSEEPWTFAVHRVMF
                     REMSVLGSLGCPSADYPTILQMVLAGKIRLEPVVGDRLPLDQVNEALARLEAGTVAGR
                     QLIVP"
     gene            1169..>1834
                     /locus_tag="VEW91_06320"
     CDS             1169..>1834
                     /locus_tag="VEW91_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYM91232.1"
                     /translation="MPPGSDISVRGEGTVASVLLSRPPLNILNLAMMEELSSHLERLA
                     ADEALGVVVLESELKDVFSAGADVREHLPATAERLIRTLERVVLQILAFPRPTVAIVR
                     GKCLGGGMELAMACDFVLASDRATFGQPEISVGVFPPVATALYPRLVGLRATYDVVLT
                     GRTLTAAEAASMGFVTSVHSEAELDAARQNFLGTLTSKSRAALQQAKRAALEGYSLWL
                     EQAM"
BASE COUNT          274 a          609 c          650 g          301 t
ORIGIN      
        1 gacgagatcg gccgcggcct cgacccacgg tatccctacg gcccgctcag cgggcgctgt
       61 ccaacctgcg gggccgccca cctgccgcag tcgtccgcgt tctgtttgaa atgcggggca
      121 aagctcacgg gggccacgcc gtgagggcgg cgctcttcca cggtcccggg aagccgctga
      181 cggtccagga ggtcgagact ccgaagatcg gcccggggga ggccctcgtc aaggtggcgg
      241 cttgcggggt gtgcgcgacc gacctccact acctgcacgg gacgccgacg ttcaagaagc
      301 cacccctgat ccttggccac gaggtctccg gtcggatcga ggcagtcgcg ccggatatca
      361 agggcttggc gccgggggag tccgtgctcg tgcccgcggt cctctcgtgc ggggaatgcc
      421 tgaactgtcg acggggtcgg gacaacctct gcgagcgcat ggtgatgccg ggcaaccacg
      481 cggacggcgg gttcgccgaa ttcctgaagg tcccggcgcg gacgctggtc ccgcttccgc
      541 ccgggctgcc actgctggag agtgcggtga tctcggacgc ggtgtcgacg cccttccacg
      601 cggtgcgaaa ccgtggccac gtccaaggtg gcgactgggt cgccatcttc gggtgcggcg
      661 gggtcgggat caatgcggtc cagattgcgg cggccctcgg ggcgaccgtg atcgcggtcg
      721 acgtcgaccc gaagaaactc gcccaggcgc gtgagctcgg agcggcggag gcgctcaacg
      781 cgaaggaagg ggatgtaccc aagaccatcc ggaagctgac gggaggcggg gtcgacgtgg
      841 cctttgagat cgtcggcaag ccgcaggtgc tggaccaggc gctcggctcc gtccgcgcgg
      901 ggggaaccct ggtggcggtc gggtacagcg aggagccgtg gacctttgcg gtgcatcgtg
      961 tcatgttccg cgagatgtcc gtcctgggct ccctgggctg cccctcggcc gactacccca
     1021 cgattctcca gatggtcctc gcggggaaga tccggctcga gcccgtcgtg ggggaccgac
     1081 ttcctctgga tcaggtcaac gaggcgctcg cgaggctgga agcaggcacc gtcgcggggc
     1141 ggcagctcat cgttccgtag gtggattcat gcctcccggg agcgacatca gcgtccgagg
     1201 ggaaggcacg gtggcgtccg tcctcctctc gagaccgccg ctgaacatct tgaacctcgc
     1261 catgatggaa gagttgtctt cgcacctcga gcggctggcg gccgatgagg ccttgggggt
     1321 cgtcgtcctc gagtccgagc tgaaggatgt cttcagcgcg ggagccgacg tgcgggagca
     1381 cttgcctgcg acggcggagc gcctcatccg cactttggaa cgcgtcgtcc tccagatcct
     1441 cgcgttcccc cggcccaccg tcgccatcgt gcgggggaag tgcctcggag gcggcatgga
     1501 gctcgccatg gcgtgcgact tcgtcctcgc gagcgaccgt gcgacgttcg gccaacccga
     1561 gatttctgtg ggggtcttcc ctcccgttgc gacggccctc tacccgcgcc tcgtcggact
     1621 ccgggccacg tatgacgtcg tgctcaccgg gagaaccctc accgctgccg aggccgcctc
     1681 catgggcttc gtgacctccg tgcacagcga agcggaactc gacgccgccc ggcagaattt
     1741 cctagggaca ctgacctcca agagccgggc cgccctccag caggcgaagc gcgccgcact
     1801 ggaaggctac tccctgtggt tggaacaggc catg
//
LOCUS       DASQGH010000324         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_26847, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000324 DASQGH010000000
VERSION     DASQGH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26847"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="selA"
                     /locus_tag="VEW91_06325"
     CDS             complement(<1..1324)
                     /gene="selA"
                     /locus_tag="VEW91_06325"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYM91233.1"
                     /translation="MEGTPLRQLPSVEHLLQRLEADGALAAYPRGLVVAATRDAVARA
                     RTELREGRAPAASVSVDGLIAEVRALLARRAAPSLVPAVNATGIIIHTNLGRAPLCDA
                     AREAAAAAGGYAVLELDPRSGDRTSRQRHVQALLCELTGAEAACAVNNNAAAVLLSLA
                     ALARGRPVIVSRGELVEIGGSFRVPEIMAVGGCRLVEVGTTNKTYLRDYEAALTPDTA
                     LLVKVHRSNFAMRGFVHEVAPQELAALGRRAGVPVLFDMGSGALVDLTTRGLPTEPTA
                     QEAIAAGVDVVTFSGDKLLGGPQAGLIIGREAPLSRIRSHPLARAVRIDKLDLAALEA
                     TLRVYRDHDRAWESIPVLRMLGADPAALERAAQGLAARLRAALGEAATVEACPTIAEA
                     GGGALPGVELPSWGVAVHPSGASPEAWIRALRRSQPPVFCRIAGDRLIV"
     gene            complement(1344..1571)
                     /locus_tag="VEW91_06330"
     CDS             complement(1344..1571)
                     /locus_tag="VEW91_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYM91234.1"
                     /translation="MRCQKCGAENPAGKIICRACGARLRPVSAGAAPLLPGSIESDEA
                     LRRRLSYDLLRIVWVIAVVIVVGLGLGMLLR"
     gene            complement(1646..2503)
                     /locus_tag="VEW91_06335"
     CDS             complement(1646..2503)
                     /locus_tag="VEW91_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYM91235.1"
                     /translation="MRIDLHTHSTASDGLLSPARLVVAARKHGVTTLALTDHDTTDGL
                     AEAAAAGQTCGVDVIPGVEINTDVGEHEVHILGYFLDYARPTLQAFLRRMREGRLTRA
                     EEMVRRLASLGAPIEWARVGEIAAGASVGRPHVARALVEAGWVGSIREAFERFIGRHG
                     PAYVPRLKLAPEEAVEIIARHDGVPVLAHPGWTASGPILERLPELVGRGLAGIEVYYP
                     DHSPEMVVAYRRLADRYGLVATGGTDYHGGGPTTRAPLGSVPVPPEVVPALRARWMSI
                     RAGRVVRDA"
     gene            complement(2513..>2767)
                     /locus_tag="VEW91_06340"
     CDS             complement(2513..>2767)
                     /locus_tag="VEW91_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HYM91236.1"
                     /translation="VLKVAAKSNPTAVAGALAGVLREKGTAELQAIGAAAINQAVKAI
                     AIARGYVAPSGLDLVCVPAFADVQIDGEDRTAIKLIVSPR"
BASE COUNT          402 a         1018 c          981 g          366 t
ORIGIN      
        1 ccacgatcag ccggtccccg gcgatccggc agaacaccgg gggctggcta cggcgcagcg
       61 cccggatcca cgcttccggg gacgcaccgg atggatggac cgccaccccc cacgagggca
      121 gctcgactcc ggggagcgct cctccgcctg cctccgcgat cgtggggcac gcttcgaccg
      181 tcgccgcctc cccgagggcg gctcggaggc gtgcggcgag accttgggcg gcgcgctcga
      241 gcgcggcggg gtctgcaccc agcatccgca gcaccgggat cgattcccag gcgcgatcgt
      301 ggtcccggta gacgcgcagg gttgcctcaa gcgccgccag atccagcttg tcgatccgaa
      361 ccgcccgggc cagcgggtgg gatcggatgc ggctgagggg ggcttcccgc ccgatgatca
      421 gcccggcctg tgggccgccc agcaacttgt cgccgctgaa ggtgaccacg tcgaccccgg
      481 cggcgatcgc ctcctgcgcg gtcggttccg tggggagccc gcgggtggtg aggtcgacga
      541 gcgccccgct gcccatgtcg aacagcacgg gcacgccggc gcgccggccg agcgccgcca
      601 gctcttgggg ggcgacctcg tgcacgaatc cgcgcatcgc aaaattgctc cggtggacct
      661 tgacgagcaa cgccgtgtca ggggtaagcg cggcctcgta gtcgcgcagg taggtcttgt
      721 tggtggtccc gacctcgacg agacggcagc cgccgaccgc catgatctcg ggaacccgga
      781 agctgccgcc gatctcgacg agctctccgc gggagacgat caccggacgc ccccgggcga
      841 gcgcggcgag cgacaacagg accgcggccg cattgttgtt gacggcgcag gcggcctcag
      901 ctccggtcag ttcgcacagg agcgcctgga cgtgccgctg ccgggaggtg cggtccccgc
      961 tgcgcgggtc cagttccagc accgcatacc cgccggccgc ggcggctgcc tcgcgcgcgg
     1021 catcgcacag gggggcgcgt ccgaggttgg tgtggatgat aatgccggtg gcgttgaccg
     1081 cgggcacgag gctgggggcg gcccgccggg caaggagcgc ccgcacttcg gcgatcagcc
     1141 cgtccacgct gaccgaggca gcgggggccc ggccctcacg cagctcggtc cgggcccgcg
     1201 cgaccgcgtc gcgcgtggca gcgacgacga ggccccgcgg atacgctgcc agtgccccgt
     1261 cggcctcaag gcgctggagg aggtgctcga ccgaggggag ctgcctgagc ggcgtaccct
     1321 ccatcgcccc cacgctcggc ggcctaccgc aggagcatcc cgaggcccag cccgacgacg
     1381 atcacgaccg ctatgaccca gacgatccgc agcaggtcgt agctcagccg tcggcgcaag
     1441 gcctcatcgc tctcgatcga gccaggaaga agcggcgcgg cccccgcgga gaccgggcgg
     1501 agccgtgcgc cgcaggcgcg gcagatgatc ttccccgcag ggttctcagc cccacacttc
     1561 tgacaccgca tcgtcaccct ccgcctgccc tgcctgtccc ccgtatgggg gggccccgca
     1621 tggggggcta acggtcccgg agcgatcacg catcccgcac cacccggccg gcccggatgg
     1681 acatccaccg cgctcggagc gccggcacga cctcgggggg taccgggacg ctcccgaggg
     1741 gggcgcgggt ggtcggcccc ccgccatggt agtccgtccc gcccgtcgcg acaaggccgt
     1801 accggtcggc caggcgccgg taggccacaa ccatctccgg ggaatgatcg ggatagtaca
     1861 cctcgatccc cgcgagcccg cgcccgacca gctcggggag ccgctcgagg atgggaccgc
     1921 tggcggtcca tcccgggtgc gccagcaccg gcacgccgtc gtggcgggcg ataatctcca
     1981 cggcttcctc gggggccagc ttgagacgcg gcacataggc cggaccgtga cggccgatga
     2041 accgctcaaa cgcctcccgg atcgagccga cccatcccgc ctcgacgagg gcccgcgcga
     2101 cgtgggggcg gcccaccgaa gcgccggcgg caatctcgcc gacccgcgcc cactcgatcg
     2161 gggccccgag gctggcgagc cggcggacca tctcctccgc ccgggtgagg cggccttcgc
     2221 gcatccgccg aagaaacgcc tggagcgttg ggcgggcata gtccaggaag tatcccagga
     2281 tgtggacctc gtgctcgccc acatcggtgt tgatttccac gccggggatc acgtcgacgc
     2341 cgcaggtctg cccggccgcc gccgcctccg ccaggccatc tgtcgtatcg tgatcggtga
     2401 gcgccagcgt ggtgacgccg tgcttgcgcg cggcgaccac caggcgcgcg ggagagagaa
     2461 gaccgtccga tgccgtgctg tgggtgtgaa ggtcgatccg caggcgtttt gcctaccgcg
     2521 gagacacgat caacttgatc gcggtccggt cctcaccgtc gatctggacg tccgcaaagg
     2581 cgggcacgca gaccagatcg agcccgctcg gagcgacgta ccctcgagcg atcgcgatcg
     2641 ccttgaccgc ctggttgatc gcagccgcgc cgatcgcctg cagctccgcg gtgccctttt
     2701 cccggaggac gcccgcgagc gctcccgcca cggctgttgg attcgatttg gcggcgacct
     2761 tgagcac
//
LOCUS       DASQGH010000325         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18461, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000325 DASQGH010000000
VERSION     DASQGH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18461"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..751)
                     /locus_tag="VEW91_06345"
     CDS             complement(35..751)
                     /locus_tag="VEW91_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008758623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYM91237.1"
                     /translation="MKVRGGRNLYGHPIGILVLDTVFPRIPGDIGHAGTFPFPVLYHK
                     VRNAFPSRVVREGDPALLEGFVAGARALEASGVLAITTSCGFLAMFQRQLAEAVRVPV
                     FTSALQLVPLVSRMLGPARAIGILTVEGRALGPRHLAGAGITEDINVVISGLERGHAF
                     TPVLLENETELDVEAARRENVEAAKEMVERHPEVGAIVLECANMPPYAAAIRDATGLP
                     VFDITTLIRMVHAALVPPVY"
     gene            complement(762..1580)
                     /locus_tag="VEW91_06350"
     CDS             complement(762..1580)
                     /locus_tag="VEW91_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91238.1"
                     /translation="MARVLRRVRAVTAAFGRAGPPFSLERAAARCGITEIRRVDPRGA
                     ARLTLEGEAGSPILAVDRRLPPHTPQWNGLVALTLARMLLPPGVTGAGADELAEIGAA
                     ELLLPARAFRPLAARTDLTMDGLRELAHRFSAPIRLTVRQWLLTGTWAGFALLWREEA
                     SGVRLRWRAASPGVRYPASLGVGARAEAVWRETARLRATLLTGRPHHGVEEVRAQAGA
                     AWWFTRFGLVREDGGRAVLALVVLERPGAPEGIAPGAANKWPTAGRRSLARRPR"
     gene            1701..2183
                     /locus_tag="VEW91_06355"
     CDS             1701..2183
                     /locus_tag="VEW91_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91239.1"
                     /translation="MRSGILLFWTGVTVTYLVLTLSAFSTTFDVDVVPAAAGVAVAER
                     VDQGPVSRLLAPGLGTLGTLRFTKRNWGFVATYTQELTVTAARLAQQAGTPVGVSVTL
                     QIPGTVVGTNATGRDNRTLVWSEIPPEGPLWARTRTTSWPAALLAAAGLAVTLWARVR
                     "
     gene            2299..>3009
                     /locus_tag="VEW91_06360"
     CDS             2299..>3009
                     /locus_tag="VEW91_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM91240.1"
                     /translation="MSRPSPPPRAVVLASGSAGNALLLEAGATHLLVDTGLSADTIER
                     TLGDLGVGMHAISAILLTHEHDDHARGAGPLSRAFGVPVMATPATLAAAGTDLAGASV
                     APFETTRPFQVGPFEVMAFPVSHDAADPVGFAFRVGRHRIAVATDLGSAGEALDPYLA
                     GADLVILESNYDLRLLNVSGYPWFVKNRILGPRGHLSNDAAAHALARTAAGRARTVCL
                     VHLSEVNNLAALARDTALA"
BASE COUNT          447 a         1133 c         1046 g          383 t
ORIGIN      
        1 aacgccgaaa aattccgtgc tgcgtcaccg cggatcagta caccggagga accagcgccg
       61 cgtgcaccat gcggatcagg gtggtgatat cgaacaccgg cagaccggtg gcgtcccgga
      121 tcgcggccgc gtacgggggc atgttggcac actccagcac gatggctccc acctccggat
      181 gccgctcgac catctccttg gccgcctcga cgttctccct ccgggccgcc tcgacgtcca
      241 gctccgtctc gttctcgagg agcaccgggg taaacgcgtg tccccgctcg agtcccgaga
      301 tgaccacgtt gatatcctcg gtgatgccgg cccctgccag gtgccggggg ccgagcgcgc
      361 gcccctccac ggtcagaatc ccgattgcgc gggccggtcc gagcatccgg gagaccagcg
      421 gcaccagctg aagcgccgac gtgaagaccg ggacccgcac ggcttccgcg agttgccgct
      481 ggaacatggc gagaaacccg cacgaggtgg tgatcgcgag gacgccgctc gcctcgagcg
      541 cacgggcgcc ggcgacgaac ccctccagaa gcgcggggtc gccttcccgc accacgcggg
      601 acggaaacgc gttgcgcacc ttgtggtaga ggacggggaa cgggaacgtc cccgcgtggc
      661 cgatatctcc tggaatcctg gggaacaccg tatcgaggac gaggattccg atgggatggc
      721 cgtagaggtt cctgccgccg cgcaccttca tgatgccgcc ctcaccgcgg acgccgggcc
      781 agagaacggc gtccggccgt gggccactta ttcgcagcgc cgggagcgat tccctccggc
      841 gcgccaggcc gttcaaggac aacgagcgcc agcacggccc gccctccgtc ctcgcggacc
      901 agaccaaacc gggtgaacca ccacgccgca cccgcctgcg cccgcacctc ctcgacccca
      961 tggtgcggcc ggccggtaag gagcgtagcg cgcagccggg cggtctcccg ccagaccgcc
     1021 tccgcacgcg cccccacacc cagcgaagcc gggtaccgaa cccccggcga ggcggcccgc
     1081 cacctgagcc gaaccccgga ggcctcctcg cgccagagca gcgcaaagcc cgcccacgtc
     1141 ccggtcagga gccactgccg gacggtcaga cggatggggg cggagaaccg atgggcgagc
     1201 tcccggagcc cgtccatcgt caggtcggta cgcgcggcga gcggccggaa ggcacgggcg
     1261 gggagcagta actcggctgc cccgatctcc gccagttcgt ccgcgcccgc accggtcacg
     1321 cccggaggaa gcagcatgcg cgccagggtc agcgcaacga gcccattcca ctgcggggtg
     1381 tgcggcggga gcctgcggtc cacggccagg atgggggatc ccgcttcgcc ttccagggtc
     1441 aggcgcgcgg ctccccgggg gtccacccgg cggatctccg tgatcccgca ccgcgcggcg
     1501 gcgcgttcca gagagaacgg gggaccggcc cgcccgaacg ccgcggtcac cgcgcgcacg
     1561 cggcgcaaca cccgggccac cagccgtgga tccacgggtt gtcagttcgg gccgttcgcc
     1621 ggtcggccct ccggccgggc agcggaggac caccccgggc agcagcgaag cacaagcggg
     1681 cagcagggag ggcacagccg atgcgcagcg ggattctgtt gttctggacc ggcgtcaccg
     1741 tcacctatct cgtcctcacg ctcagcgcct tctcgacgac gttcgacgtc gatgtcgtcc
     1801 ccgccgccgc cggcgtcgcg gtggcagaac gcgtggacca gggaccggtg agccggctgc
     1861 tcgctcccgg cctcgggacc ctcgggacgc tgcggttcac aaaacgcaac tggggcttcg
     1921 tggcgaccta cacgcaggag ctcacggtca cggcggcccg cctggcccag caggccggca
     1981 cgccggtcgg cgtcagcgtg accctgcaga tccccgggac ggtcgttggc accaacgcca
     2041 cggggcggga caaccggacg ctggtgtgga gcgagatccc gcccgaaggc ccgctgtggg
     2101 cgcgcacgcg gaccacgagc tggccggcgg cgctcctggc cgccgccggc ctcgcggtga
     2161 cgctgtgggc acgggtgcgc tgacggtccg ggtgaggtcg agggaccccg agggcgcgcc
     2221 tcgaacccta tacggtgatg cgcccgttcc tccatcgcca tctccccgcg ccttgacgcg
     2281 cctcggggac tcgcgatcgt gagccgcccc tccccgcccc cgcgggccgt cgtgctggcg
     2341 agcgggagcg cgggcaatgc cctgctgctc gaggccggcg cgacccatct gctcgtcgac
     2401 accggcctct cggccgacac gatcgagcga accctcggcg acctgggggt gggcatgcac
     2461 gcgatctcgg cgatcctcct gacccacgag cacgacgatc acgcccgcgg ggcgggcccg
     2521 ctcagccgcg cgttcggggt cccggtgatg gccacccccg ccaccctggc ggccgccggg
     2581 accgacctcg cgggcgcgtc ggtcgccccc ttcgaaacga cccggccgtt tcaggtgggg
     2641 ccgttcgagg tcatggcgtt tccggtctcg cacgatgcgg cagaccccgt cgggttcgcc
     2701 ttccgggtcg gccggcaccg gatcgcggtc gccaccgacc tcggcagcgc cggcgaggcg
     2761 ctagacccgt acctggccgg ggccgacctc gtcatcctgg agtcgaatta cgacctcagg
     2821 ctattgaacg tcagcgggta tccgtggttt gtgaagaacc ggatcctggg gccccggggc
     2881 cacctcagca acgacgccgc cgcccatgcg ctcgcgcgga cggcggcggg gcgcgcccgc
     2941 accgtgtgcc tcgtccacct gagcgaggtg aacaacctgg cggcgctcgc ccgcgacacg
     3001 gcgctggcg
//
LOCUS       DASQGH010000326         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4312, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000326 DASQGH010000000
VERSION     DASQGH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4312"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..926
                     /locus_tag="VEW91_06365"
     CDS             66..926
                     /locus_tag="VEW91_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91241.1"
                     /translation="MASGRARRFLRGLLGAPLAALGVGVVLAYIGVAGGAPWLAPYDP
                     LVQDLSAALAPPSPAHPFGTDPLGRDLLSRVIAASRVDLTIAATGTGVASALAVPLGL
                     ASAYAGGAIDRAIAAALDSALTFPTLLLAVVLVNLVGNGLGTVIGAVAVTATPALFRV
                     VRGAVLQIAVAGFVDSARAAGAGSLRIIWRHLLPNIVGQAAVYATLLASQAVLTVTAL
                     GFLGLGVQPPTPEWGAMLARSRTYLSAAPFVMLFPGLAIGGLVLGLNLLGDALQDALD
                     PRGTLRRAAF"
     gene            complement(923..1558)
                     /locus_tag="VEW91_06370"
     CDS             complement(923..1558)
                     /locus_tag="VEW91_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM91242.1"
                     /translation="MEQLERRTSPVVTPPSPVELGSPAKPALGQPPNAAEEALFNEMA
                     AQHLDALYRTALRLTGRPQDAEDLVQETYLRAWRSLHTYRPGTNPKAWLFRILHNAHI
                     DRFRASSRTVPTVDELEGQDPAFVVHETPETLVMAGLMDAEVRQALAEIPEVFRACLV
                     LADLEGFSYQEIAEILSIPRGTVMSRLFRGRRAMRRLLTQYGRDHGYSKAG"
     gene            complement(1564..>1822)
                     /locus_tag="VEW91_06375"
     CDS             complement(1564..>1822)
                     /locus_tag="VEW91_06375"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HYM91243.1"
                     /translation="AELGGVHPEFRRHLSEFFERWERSVDRCLQDGVARGEFPTDLDT
                     RPLATALISQIEGAILLMKTHRHGGPIEAGVRTMLRLLEGR"
BASE COUNT          222 a          649 c          641 g          310 t
ORIGIN      
        1 tgggttcctt gtcttgaacc tgctcgtgga tctctcgtat gcgctgtggg atccgcgcgt
       61 gcgctatggc tagcgggcgc gcccggcgat tcctccgcgg cctgctcggc gcgccgctcg
      121 ccgcgctcgg ggtcggggtc gtcctagcct acatcggggt cgccggcgga gccccgtggc
      181 tcgcacccta cgacccgctc gtgcaggacc tgtccgcggc cctcgctccc cccagccccg
      241 cgcacccctt cgggaccgat ccgttggggc gcgacctgct cagccgggtg atcgccgcct
      301 cgcgcgtgga tctcacgatc gccgcaaccg ggaccggcgt cgcctccgcc ctggccgtcc
      361 cgctggggct cgcctccgcc tacgccgggg gcgcaattga tcgggcgatc gccgcggcgc
      421 tggactcggc cctgacgttt cccacgctgc tgctggcggt cgtgctggtc aaccttgtcg
      481 gcaacggtct ggggacggtg atcggtgccg ttgcggtcac ggcgaccccg gccctgttcc
      541 gggtggtgcg gggcgcggtc cttcagatcg ccgtcgcagg gttcgtggac tcggcgcgcg
      601 ccgcgggtgc cggatcgctg cgaatcatct ggcgccacct cctccccaac atcgtcgggc
      661 aggcggcggt ctacgcgacg ctgctcgcga gccaggcggt cctgacggtg acggccctgg
      721 ggttcctggg gctcggggtg cagcccccca ctcccgaatg gggcgcgatg ctggcgcgga
      781 gccgcacgta cctcagcgcc gccccgtttg tgatgctctt ccccggactg gcgatcggcg
      841 gtttggtcct cgggctgaac ctgctcggcg acgccttgca ggacgccctc gatccacgag
      901 ggacgctgcg gcgggcggcg ttctagccgg ccttcgagta cccgtggtcg cgcccgtact
      961 gggtgagcaa gcggcgcatc gcgcgccgcc cccggaatag gcggctcatg accgtgcccc
     1021 ggggaatcga caggatctcc gcgatctctt ggtacgagaa cccctcgaga tcagcgagga
     1081 cgaggcaggc ccggaacacc tcgggaatct ccgccagcgc ctggcggacc tcggcatcca
     1141 tgaggcctgc catcacaagc gtctcgggcg tctcgtggac gacgaacgcg ggatcctggc
     1201 cttcgagctc gtcaaccgtc gggaccgtgc gagacgacgc ccggaaccgg tcgatgtgcg
     1261 cgttgtggag gatgcggaac agccaggcct tggggttggt gcccggccgg tacgtgtgca
     1321 gggagcgcca cgcgcgcaga taggtttcct gaacgagatc ctccgcatcc tgcgggcggc
     1381 cggtcagccg gagcgcggtc cggtacagcg cgtccaggtg ctgggcggcc atctcgttga
     1441 acagggcctc ctccgctgcg ttcgggggct gcccaagcgc gggcttggcc gggctcccca
     1501 gctcgaccgg cgagggcggg gtgaccaccg gggaggttct gcgctcgagt tgctccatgg
     1561 agttcaccta ccttccagca gcctcagcat cgttcggacc cctgcttcga tcggtccccc
     1621 gtgccggtgg gtcttcatga gcaggattgc gccttcgatc tggctgatca gcgcggtggc
     1681 cagcggccgg gtgtcgagat cggtcgggaa ctccccccga gccacgccgt cctggaggca
     1741 ccgatccacc gaccgttccc accgctcgaa gaactcgctc aggtggcggc ggaactcggg
     1801 atggactccc ccaagctccg cg
//
LOCUS       DASQGH010000327         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52324, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000327 DASQGH010000000
VERSION     DASQGH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52324"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VEW91_06380"
     CDS             complement(<1..616)
                     /locus_tag="VEW91_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM91244.1"
                     /translation="MREAAAIRVGAPSAAPDVVRRRPWLRFVRRRTAVAGGIITPLFV
                     LVMLLGDWIRPFPPAAVDYDHTMARPSLAHLAGTDDFGRDIMSRLIAGSRYSLGMGLG
                     ATLVGTVMGSAWGIASAFYGRGFDNVSMRVADVLLAFPGILLAIAIVAIIGPGLTNVV
                     IAVGVFTVPIFARLARGPALGAMAQEYIQAAHAMGAGNVPIMARH"
     gene            complement(609..1529)
                     /locus_tag="VEW91_06385"
     CDS             complement(609..1529)
                     /locus_tag="VEW91_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017346955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM91245.1"
                     /translation="MLRYAGRRLWQMIPILLGVSLGVFAFVHAIPGDPARLLAGPDAT
                     PLDVAAVRVQLGLDRPLPVQYLYFVANALAGNFGRSFRSGEPVIRVVATHVTPTLILS
                     MASMLLAAAMGLSVGVLQAVRRNSLSDYVSTIVSVAGISTPAFFLGLLLIFLFSVDLR
                     WFPTGGMHGAVSLVLPAVTLAAGSAAQIARFARSSLLDVVGEDYVRTARAKGAAERRV
                     LLHHSLRNALIPVVTLIGLQFGFLLGGAIVVETVFSWPGLGWLLIQSISFRDYPVLTA
                     EILLFSLQFLAINLVVDLLYAALDPRVGYA"
     gene            complement(1564..>1913)
                     /locus_tag="VEW91_06390"
     CDS             complement(1564..>1913)
                     /locus_tag="VEW91_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881596.1"
                     /note="with GsiACD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HYM91246.1"
                     /translation="NQSQVNLAGWSPSTGDADWGLRPLFGGESWPPTLFNLAFYKNPK
                     VDALINDALATADQTRRNTDYAQAMRIIWDDAPWVFLHNPQNLSGVRTSVTDVYALPD
                     GTVDVRQAALTGK"
BASE COUNT          348 a          657 c          670 g          238 t
ORIGIN      
        1 gatgccgggc catgatgggg acgtttccgg ctcccatcgc gtgggcggcc tggatgtact
       61 cttgggccat cgccccgagc gcggggccgc gcgccagccg cgcgaagatg gggacggtga
      121 acacgcccac ggcgatcacg acgttggtga gccccggccc aatgatcgcc acgatcgcga
      181 tggccagcag aatccccggg aacgccagca ggacatccgc cacccgcatc gagacgttgt
      241 cgaaccctcg gccgtagaag gcactcgcga tcccccacgc cgatcccatg accgttccga
      301 cgagcgtcgc gccgaggccc atcccgagcg agtaccgcga tccggcgatc aaccggctca
      361 tgatgtcccg cccgaagtcg tcggttccgg cgaggtgggc gagcgacgga cgcgccatcg
      421 tgtggtcgta gtcgaccgca gctggcggga acggacggat ccaatcgccg agcagcatca
      481 ccaggacgaa aagtggcgtg atgatgcccc cggccacggc ggtgcgccgg cgcacgaacc
      541 ggagccacgg ccgccgccgg accacgtcgg gcgcggccga gggcgccccg acccggatgg
      601 cggccgcttc acgcatagcc tacccgcggg tcgagcgccg cgtacagcag gtccacgacg
      661 aggttgatgg cgagaaactg caaggagaac agcaggatct ccgccgtgag caccgggtag
      721 tcgcgaaacg agatcgactg gatcagcagc cacccaagcc cgggccagct gaacacggtc
      781 tccacgacga tcgcgccgcc gaggaggaac ccgaactgca gcccgatgag cgtgacgacg
      841 gggatcagcg cgttccgcag ggagtggtgg agcagcacgc gtcgctccgc ggctcccttg
      901 gccctggcgg tccgcacgta gtcctcgccg accacatcca ggaggctgct ccgcgcgaac
      961 ctcgcgatct gggcggcgga gccggcggcg agcgtcaccg ccgggagcac gaggctgacc
     1021 gcgccatgca tcccgccggt gggaaaccag cggaggtcga ccgagaacaa gaagatgagg
     1081 aggagcccca ggaagaacgc cggcgtgctg atgccggcga ccgacacgat ggtgctcacg
     1141 taatcggaga gcgagttccg gcggaccgcc tggagcacgc cgacgctcaa ccccatcgcc
     1201 gccgcgagga gcatgctggc catggaaagg atgagcgtcg gggtgacgtg ggtcgccacc
     1261 acacggatga ccggctcccc gctccggaac gagcgcccga agttgccggc cagggcgttt
     1321 gcgacgaagt agaggtactg cacgggcagc ggccggtcga gcccaagctg gacgcggacc
     1381 gccgccacat cgaggggagt cgcgtccgga cctgcgagca gccgcgccgg gtcgccgggg
     1441 atcgcgtgga cgaacgcaaa cacgccaagc gagaccccga ggagaatcgg gatcatctgc
     1501 cagagccgcc gaccagcgta ccggagcatc ggcggctagg gccgctcggc cgccccgcgg
     1561 gtcctacttg ccggtcaggg ccgcctgccg gacgtccacc gtcccatcag gcagcgcgta
     1621 gacgtccgtc acgctggtcc gcaccccgga caggttctgc gggttgtgca gaaacaccca
     1681 cggcgcgtcg tcccagatga tccgcatcgc ctgcgcatag tccgtgttcc gccgggtctg
     1741 gtctgccgta gcgagcgcat cgttgatcag ggcatcgacc ttggggttct tgtagaacgc
     1801 caggttgaac agcgtgggcg gccaggattc gccgccgaag agcggcctca ggccccagtc
     1861 ggcatcgccc gtggacggcg accacccggc gaggttgacc tgcgactggt tct
//
LOCUS       DASQGH010000328         1792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7159, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000328 DASQGH010000000
VERSION     DASQGH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7159"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..144)
                     /locus_tag="VEW91_06395"
     tRNA            complement(69..144)
                     /locus_tag="VEW91_06395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Ala,seq:cgc)
     gene            264..1607
                     /locus_tag="VEW91_06400"
     CDS             264..1607
                     /locus_tag="VEW91_06400"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HYM91247.1"
                     /translation="MTRETIALTQVEQRRAIVLTQLGEHRIDAAHAAALLDLSVRHCR
                     RLLAAFRRRGPAALAHGNRGRPASNRVPRALERRIVRLARTRYVGFNHQHLTEKLAED
                     HAIVLSRPTVHRILLAAGLPSPHPRRRRRFRRRRERMPLPGLLLQWDGSHHDWLQGRG
                     PRLVLHGAIDDATSDVPAAIIRTQEDAQGYFTVLRQLVCTHGIPVALYCDRHGIVTND
                     ARPRPLTLNEQLRGHTRPPTQVGRALHELGIQWIPAHSPQAKGRIERLWGTFQDRLVS
                     ELRLAQARTLEEANAVLRAFLPRYNARFTRPAAQPGSAYRALPPDLNLDDVCCFAYER
                     TVANDNTVTLDAQRVQLLPDAHRASYAKGRVIVRRHLDGRYSVSHGRRRIAFRILTAP
                     PHPAPSEKPTAGRPVRPRVPSSWKPSPDHPWKKTYAQEQRRKQLRMAGVTFSLNA"
BASE COUNT          329 a          642 c          530 g          291 t
ORIGIN      
        1 gtttgggcac agagtggaat cttcaggtgg cctgggtctc acgaactccg cgtcaggtgc
       61 gggtttcctg gtggagacga tgggattcga acccacgacc tccgcaatgc gagtgcggcg
      121 ctctcccaac tgagctacgt ccccaccgac aacggtatta taggcgggcg atatgttccg
      181 ggcaacaaca accccacaga catgtggatg aaaagcgata atgtcacgcc ataagcgaaa
      241 agcgaatgtg tcaccctgga ggcatgacca gggagacgat cgccttgaca caggtcgaac
      301 aacgacgcgc aattgtgctg acccagctag gggagcaccg gatagacgcg gcgcacgctg
      361 ccgccttgtt ggacctctcg gtgcgccact gtcggcgctt gctcgccgct ttccgccgca
      421 ggggacccgc cgctttggct catgggaatc gtggccgccc cgcctccaat cgcgtgccac
      481 gcgctcttga gcgccggatc gtgcgcttgg cccgaacgcg gtatgtcggg ttcaatcacc
      541 agcacctgac tgagaaactc gccgaggacc acgccatcgt cctctcgcgc cctaccgtcc
      601 atcggatcct gctggccgcc gggctcccca gccctcaccc ccgccggcgc cgtcgctttc
      661 gccgccgccg ggagcgcatg cccctgcccg gtctgctcct ccaatgggat ggcagccacc
      721 atgactggct ccagggacgc ggacctcgcc tggtcctgca cggcgccatt gacgatgcca
      781 ccagcgacgt ccccgccgcc atcatccgca cgcaggaaga tgcccagggc tacttcaccg
      841 tgctcagaca gctggtctgc acccacggaa ttcccgtcgc cctctattgc gatcgccacg
      901 gcatcgtcac caatgacgcc cgtccccggc ccctcacgct caacgagcag ctccgcggtc
      961 acacccggcc cccgactcag gtcggtcgcg ccctccacga gttagggatc caatggatcc
     1021 ccgctcactc cccgcaagcc aagggccgca tcgaacggct ctgggggacc ttccaagatc
     1081 ggctcgtcag cgaactccga ctcgcccagg cccgcaccct cgaggaagcg aacgcggtct
     1141 tgcgggcctt tctgccgcgg tacaatgcgc gctttacacg ccccgccgcc caacccgggt
     1201 cggcctaccg ggctctgccg ccggacctca acctcgacga cgtctgctgc ttcgcttacg
     1261 agcgcacggt ggccaacgat aataccgtca cgctcgacgc acagcgcgtc cagctcttgc
     1321 ccgatgcgca tcgcgccagc tatgcgaagg ggcgcgtcat cgtccgccgc cacctcgacg
     1381 ggcgctactc cgtgagccac gggagacgcc ggatcgcgtt tcggatcctc accgcaccac
     1441 ctcatcctgc gcccagcgag aaacccaccg ctggacgccc ggtgcgcccg cgtgtcccca
     1501 gctcgtggaa gccgtcgcct gatcatccct ggaagaagac gtatgcgcag gaacaacgcc
     1561 gaaagcaact caggatggcg ggggtgacat tttcgctgaa cgcttagggt gacagaatcg
     1621 ctgagcattg acagactgcg aaacggggtt ccatgagcac gggccccagg tggccactgg
     1681 cgagagcagg gttcctggcg ccgcgctgcg gaggagccag ttggtccgaa ttaacgctga
     1741 cacgatgcgc agaagtagct ggtccgaccg gagatacgcg cagtgcgaat gg
//
LOCUS       DASQGH010000329         5822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46781, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000329 DASQGH010000000
VERSION     DASQGH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46781"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1380)
                     /locus_tag="VEW91_06405"
     CDS             complement(73..1380)
                     /locus_tag="VEW91_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYM91248.1"
                     /translation="MPSAASPAVPAAQPIPRLAIPELDRCIQCGFCLQHCPTYRITGM
                     ETESPRGRIHLVEAAAQGRIPIDARFEEHMYVCLGCRACETACPSGVKFGTIIEAARA
                     EIGPTGSPVARALTQLALRHLLPFPGRLRLGATLLRVYQRSGLGKRARALHLVPLRLA
                     EMEALLPPIPNRFFAPPQEVFPAIGPRRARVGVLSGCVMSIIFADLNEATIRVLQRNG
                     CEVVVPRGQTCCAALNLHNGERDRAREMARRNIDAFLGAGVDAIIINAAGCGSTSKEY
                     DALLRDDPAYAEKAQRYSRLCRDVTEFLAERDLTGPLGEVRARVTYQDPCHLVHGQGI
                     RRQPRELLRRIPGLDLVEMEGSDRCCGSAGIYNLVQPAYSRQILEEKMAALGRTGADL
                     VVAPNPGCMLQIASGIRASGLRMHVHHLIDLLDRAYAAAERRG"
     gene            complement(1380..2852)
                     /locus_tag="VEW91_06410"
     CDS             complement(1380..2852)
                     /locus_tag="VEW91_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYM91249.1"
                     /translation="MQLADLRHRIDDRTALTRALQDVLGPDRVFTHPADLIAYEYDGS
                     VLAAIPDLVVHPVSTEEVVGVVKAARRHRIPLIPRGSGTGLSGGAITPVGGIIVSLAG
                     MNRILHVDLDNRCAVVQPGVINLDITKAVEAGGYFYAPDPSSQSACSIGGNVANNSGG
                     VHTLAFGVTTNHVLGLTLVLPDGSVERLGGRGPDEPGLDLPGLVVGSEGTLGIVTEVA
                     LRLMRRREGVRTMLGIFETIEEASQAVADIIASGIGPTSLEMIDRLTVEAVEPALHAG
                     LPLDAGAILLIEVEGVNEVLDRSVRTVDALCRKNRAREFRLARTEEERRLYWAARKGA
                     FGAMGRLAPNYFLHDAVVPRSQLPAILHQVVEIARRYGIRVANVFHAGDGNLHPLIPY
                     DASNPEETDRVLKASEEMLAACVAAGGTLSGEHGIGFEKNNYMPWIFSASDLDAQHRL
                     KSAFDPDDLMNPFKVFPTPVSCGELLVRRAPRLATSGLWI"
     gene            3050..3319
                     /locus_tag="VEW91_06415"
     CDS             3050..3319
                     /locus_tag="VEW91_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005483762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91250.1"
                     /translation="MSGANAAIKNNTLPRLFQSTVEELKPEAAYFLPDGGKRTAYFFF
                     DLKDPSQIPVIAEPFFQSLNATVEFIPAMNADDLKVGLERWARRQ"
     gene            3512..3673
                     /locus_tag="VEW91_06420"
     CDS             3512..3673
                     /locus_tag="VEW91_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91251.1"
                     /translation="MFGLQHPRRALRRAIAKRLVLGFLMALFAIVAGWMLVPPDMGVD
                     KATVEWYTR"
     gene            complement(3699..4406)
                     /locus_tag="VEW91_06425"
     CDS             complement(3699..4406)
                     /locus_tag="VEW91_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HYM91252.1"
                     /translation="MSAGVPEDPLASLRRLRVRARVRARAGLLREAVETMSGLGFTVA
                     RLADREEVLGKVAEIVPPGANAVYQPCAVGRALHLDEALRAQGRDIVVLPADGDPGEP
                     NGAWREHFLTAQFGITGATALVADTGSLVLAEDSGFGRAASNVPPVHVALVAADCVVE
                     NLLDAATMARGYAALHLGRAAPRYLTLISGPSKTADIAMTLVRGMHGPRTVHVVIWDR
                     PKAEGSGDEALRTWVLA"
     gene            complement(4403..>5822)
                     /locus_tag="VEW91_06430"
     CDS             complement(4403..>5822)
                     /locus_tag="VEW91_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYM91253.1"
                     /translation="IVRTLEDALTVIQGASVFGVGQRVGTYASFITGPAPAGGFGPNE
                     VHVILVDNGRRRAVAKGFGEAFACISCGSCLNHCPVYAVIGDKYGVTTHIGGIGILQA
                     SFTTGLGLTSAQGLSLCLNCRACVDPCPVQIDTPGMHARLRESVPASRRLGAMARAIL
                     AITSRIPLMRASGRAVYAAERLGVRRAVEHLLPRRLQDLAPLLPPMPAPREMAPPPEV
                     LEPVGPHRHTVAFFAGCVMSTWLAPINWATFRVLARSGCRIRVPRAQGCCGALHHHMG
                     EGRQARVLARRVIEAFEGLDDCEVVLTNSAGCGAAMKEYAELLHDDPAYAERARAFAI
                     RVRDTSEFLAARGVAPAVTRIRERAVYFDPCHLSIAQGISKEPRWLLRRVPGLEMVEA
                     KRREACCGSAGLYSLIHPEVSGRLADLLVEDLCAANPDLIVTSNPGCLLQVRWGLARA
                     GEGRIRVAHVMEVLDRATGTAG"
BASE COUNT          862 a         2052 c         2053 g          855 t
ORIGIN      
        1 ccagggccgc cggctcggta atgacgtgcg cggctccaac gatctcgcac gcccagtcgg
       61 cgaggaacac ggtcatcccc gtcgctccgc ggccgcgtac gcccgatcga gcaggtcgat
      121 gaggtggtgg acgtgcatgc gcagcccgga cgcgcggatc cccgaggcga tctgcagcat
      181 gcagccgggg ttcggggcga ccacgagatc ggcgccggtg cgcccgaggg ccgccatctt
      241 ttcctcgagg atctgccggg agtacgccgg ctggacgagg ttgtagatcc cggcgctgcc
      301 gcagcaccgg tcggacccct ccatctcgac gaggtccagc ccgggaatgc ggcggagcaa
      361 ctcccggggt tggcggcgga tcccctggcc gtggacgagg tggcaggggt cctggtaggt
      421 cacccgcgcc cggacctcgc cgagcggacc ggtgaggtcg cgctccgcca ggaactcggt
      481 cacgtcacgg cagaggcggc tgtagcgctg cgccttctcc gcgtaggccg gatcgtcccg
      541 gagcagcgcg tcgtactcct tgcttgtgct gccgcacccg gccgcgttga tgatgatcgc
      601 atccacgccg gcgccgagga acgcgtcgat gttccgccgg gccatctccc gcgcccggtc
      661 gcgctcgccg ttgtgcagat tcagcgcggc gcagcaggtc tgaccgcggg ggacgacgac
      721 ctcgcagccg ttccgctgca ggacccggat cgtcgcctcg ttcaggtcgg cgaagatgat
      781 gctcatcacg cacccgctga gcaccccgac ccgtgcccgg cgcgggccga tcgcggggaa
      841 cacctcctgg ggcggggcga agaagcggtt ggggatcggc gggaggaggg cctccatctc
      901 cgccaggcgc agcggcacca ggtggagcgc gcgcgcgcgc ttccccagcc cgcttcgctg
      961 gtagacccgc agcagcgtgg cgcccagcct gagcctgccc gggaagggca gcaggtggcg
     1021 gagcgcgagc tgcgtcagcg cccgggcgac cggtgagccg gtcgggccga tctcggcccg
     1081 ggccgcttcg atgatggtgc cgaacttgac gccggaggga caggcggttt cacacgcgcg
     1141 gcacccgagg cacacgtaca tgtgctcttc gaaccgggca tcgatcggga tccggccctg
     1201 cgccgctgcc tcgaccaggt ggatccgccc gcggggcgat tcggtttcca tccccgtgat
     1261 ccgatacgtc gggcagtgct gcaggcagaa cccgcactgg atgcaccggt ccagctccgg
     1321 gatcgcgagg cgtgggatgg gctgcgccgc cggcactgcg ggggaggcgg cggagggcac
     1381 tagatccaca accccgaggt ggccaggcgc ggcgctctcc ggaccaggag ctccccgcac
     1441 gagaccggcg tggggaacac cttgaagggg ttcatgagat cgtctgggtc gaacgcgctc
     1501 ttgagccggt gctgggcgtc gagatcggac gcggaaaaga tccacggcat gtagttgttc
     1561 ttctcgaacc cgatcccgtg ctccccgctg agcgtccccc ccgcggcgac gcaggcggcc
     1621 agcatttcct cgctggcctt gagcacgcga tcggtttctt ccgggttcga cgcgtcatac
     1681 ggaatgagcg ggtgcaggtt gccgtccccc gcatggaaga cgttggcgac tcggatgccg
     1741 taccggcggg cgatctcgac gacctggtgc aagatcgccg ggagctggct ccgcgggacg
     1801 accgcatcgt ggagaaagta gttgggcgcg agccgcccca tcgccccgaa cgcgcccttc
     1861 cgggccgccc agtacaggcg tcgttcctcc tcggtgcggg cgaggcggaa ctcccgcgcg
     1921 cggttcttgc ggcacagggc gtcgacggtg cgaaccgacc ggtcgaggac ttcgttgacc
     1981 ccttccactt cgatcaacag gatcgccccg gcgtcgaggg gcaacccggc gtgcaacgcg
     2041 ggctcgaccg cttcgaccgt gagccgatcg atcatctcga gcgaggtcgg cccgatgccc
     2101 gacgcgatga tgtccgccac cgcctggctc gcctcctcga tcgtctcgaa gatgccgagc
     2161 atcgtccgga cgccctcgcg gcggcgcatc agcctgaggg cgacctcggt gacgatcccg
     2221 agcgtcccct cggatccgac cacgagcccg gggaggtcga gccccggctc atcgggcccg
     2281 cggcccccga ggcgctccac cgacccgtcg ggcaggacca gggtgagccc gaggacgtgg
     2341 ttcgtcgtca cgccaaacgc cagggtgtgc acgcccccgc tgttgttggc gacgttgccg
     2401 ccgatgctgc acgccgactg gctgctgggg tccggcgcgt agaagtaccc gccggcctcc
     2461 acggccttcg tgatgtcgag gttgatgacg cccggctgga cgaccgcgca ccggttgtcg
     2521 aggtccacgt ggaggatgcg gttcatcccg gcgagggaga cgatgatccc gccgaccggg
     2581 gtgatcgcgc ccccggagag ccccgtcccg gacccccggg ggatcagcgg gatccggtgg
     2641 cggcgcgcgg ctttgacgac cccgacgacc tcctcggtcg acaccggatg cacgacgagg
     2701 tctgggatgg cggcgaggac ggagccatcg tactcgtagg cgatgaggtc ggcggggtgg
     2761 gtgaatacgc ggtcgggccc caggacatcc tgcagcgccc gcgtcagggc ggtccgatcg
     2821 tcaattcgat ggcggagatc ggcaagctgc acgacggttt cagtatagca taccgcttcc
     2881 ggcctcgatg ggagcgcgcg ccgcgggatg cccctccacc gagcgactcg cgcacggggg
     2941 gcggagaggt aggggatgac gagcaccggc gagaggttgc tggccgccga atgggctttc
     3001 tgagcgagtg aagaggattc gatgcggatg atgttgcgcg ttccgctcga tgtcgggggc
     3061 aaatgcggcg atcaagaaca acacgttgcc gaggctgttc cagtcgacag tcgaggagct
     3121 caagcccgag gccgcttatt tcctgccgga cggcgggaag cgcaccgcct atttcttttt
     3181 cgacctcaag gacccgtccc agatccccgt cattgccgag ccctttttcc agtcgctgaa
     3241 cgccaccgtc gagtttatac cggccatgaa cgccgatgac ctgaaggttg gattggagcg
     3301 atgggcgagg aggcaatgat gccgcgcccc ggcccgggcg aggcgtaggg cgcacaaagg
     3361 ttctcgtgag ggttcgaacg aaccgcgtca atcatccgat cgaaggaggg aatggctggt
     3421 ccctccgcga tacactctga agagttgatt tgcgcggcgc ccctgtccgg cccgcaggca
     3481 cgccgccgcg gcgagcagag aggagatcgc gatgttcggt ctgcaacacc cccgtcgtgc
     3541 gcttcgccgg gccatcgcca agcggttggt gctgggtttc ctgatggcgc tcttcgcgat
     3601 cgtcgccggg tggatgctcg tgccgcccga catgggcgtc gacaaggcca ccgtggagtg
     3661 gtatacgcgc tagcctagtc taagtctagt cccccgatct aagcgagcac ccaggtcctc
     3721 agcgcttcgt cgccgctccc ctccgctttg ggccggtccc agatgacgac gtggacggtc
     3781 cgcgggccgt gcatgccccg caccagcgtc atggcgatat ccgccgtctt gctgggcccg
     3841 gagatgaggg tcaggtagcg gggcgccgcc cggccgaggt gcagggcggc gtatccgcgc
     3901 gccatcgtgg cggcatccag caggttctcc accacgcagt cggccgccac cagcgcgacg
     3961 tgcaccgggg gcacgttgct ggcggcccgc ccaaagcccg agtcttcggc caggaccagg
     4021 ctccccgtgt cggcgacgag cgcggtcgcc cccgtgatgc caaattgcgc ggtgaggaag
     4081 tgctcgcgcc aggccccgtt cggttcaccc gggtcgccat cggcggggag cacgacgatg
     4141 tcccgcccct gggcccgcag ggcctcgtcc agatggaggg ccctcccgac cgcgcacggt
     4201 tggtagacgg cgttcgctcc gggcggaacg atctcggcca ccttgccgag gacctcttcg
     4261 cggtccgcaa gcctggccac tgtgaagccc aggccggaca tcgtctccac cgcttcccga
     4321 agaaggccgg cccgcgcccg cacccgcgcg cgcaccctga gccgcctgag cgacgccagc
     4381 ggatcttccg ggacccccgc gctcacccgg ccgtccccgt tgcgcggtcg aggacctcca
     4441 tcacgtgcgc cacccggatc cgcccctccc ccgcccgggc cagcccccaa cgcacctgca
     4501 gcaggcagcc cgggttgctc gtgacgatca ggtccgggtt ggccgcgcac aggtcctcga
     4561 ccaggaggtc ggcgagccgg ccgctcacct ccgggtggat gaggctgtag aggccggcgc
     4621 tgccgcagca cgcttcccgc cgtttggcct caaccatctc cagcccgggg acccggcgga
     4681 gcaaccaccg cggctccttg ctgatgccct gggcgatgct gaggtggcag ggatcgaagt
     4741 acaccgcgcg ttcccggatc cgggtcaccg ccggggcgac gccccgcgcg gcgaggaact
     4801 cgcttgtatc gcgcacccgg atcgcgaacg cgcgcgcccg ctccgcgtaa gccgggtcgt
     4861 cgtgcagcaa ctcggcgtac tccttcatcg cggccccgca tccggcggag ttcgtgagga
     4921 cgacctcgca gtcgtccaac ccttcgaacg cctcgatgac ccgtcgagcg agtacccggg
     4981 cctgccgccc ctcgcccata tggtggtgca gcgccccgca gcagccctgc gcccgcggca
     5041 cccggatccg gcagcccgac cgcgcgagca cccggaaggt cgcccagttg atcggcgcca
     5101 gccacgtgct catcacgcac ccggcgaaga aggcgaccgt gtgccggtga gggccgaccg
     5161 gctcgaggac ctcgggcggc ggcgccatct cccggggggc gggcatcggc gggaggaggg
     5221 gcgccaggtc ctgcaggcgg cggggaagga ggtgctcaac ggcccggcgt accccgagcc
     5281 gctcggccgc gtacacggcg cgaccgctgg cccgcatcag cgggatgcgg ctggtgatgg
     5341 cgaggatcgc ccgcgccatc gcccccaggc gccgggacgc cgggacgctc tcgcgcagcc
     5401 gggcgtgcat gccgggcgta tcgatctgca ccgggcacgg gtcgacgcac gcccggcagt
     5461 tgaggcacag cgagagcccc tgcgcggacg tgaggccgag cccggtcgtg aaactggcct
     5521 ggaggattcc gatgccgccg atgtgggtgg tgaccccgta cttgtcgccg atcaccgcgt
     5581 agacggggca gtggttgagg cagctgccgc agctgatgca cgcgaacgct tccccgaacc
     5641 ccttggccac ggcccgccgc cgtccgttgt ccaccaggat gacgtggacc tcgttcggcc
     5701 cgaacccccc cgcgggcgcg ggccccgtga tgaaggaggc gtatgtcccc acccgctggc
     5761 ccaccccgaa gacgctggcc ccctggatca ccgtcagggc gtcctccagc gtgcgcacga
     5821 tc
//
LOCUS       DASQGH010000330         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46789, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000330 DASQGH010000000
VERSION     DASQGH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46789"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VEW91_06435"
     CDS             complement(<1..688)
                     /locus_tag="VEW91_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYM91254.1"
                     /translation="MNHALALLKQMLGAAVDWGYLAASPVGKVRKLRLPRRPLPLWTP
                     AELRRFLLVAPETWRPVWLVATFAGLRPGEIQAMRWTEQNWPDFTANKIHVTASYEAR
                     SKVLGAPKTDRSVRDVDMVPTVRRVLEALASRAAGGLVFPGPGGGMFARTNMKRAWAH
                     AIAQAQVRPIRPYDLRHTFASLLIAAGKNPLYIARQMGHYSAGFTLDTYGHLMESVPR
                     VQVEWINEIVF"
     gene            complement(685..1134)
                     /locus_tag="VEW91_06440"
     CDS             complement(685..1134)
                     /locus_tag="VEW91_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYM91255.1"
                     /translation="MASVQPLTDLTVADLWREVKDERTLWGDVSQQTLRTVKLLLENR
                     MHDELTDYLNAGRYVRDYGRAGYRNGVYRRRLTTTWGTIPDLQIPRARDPGFRPSVVA
                     RYQARTEQVNRLVRAVFLGGLSTRRVGAALAPLLGETVSPATVSTIT"
BASE COUNT          316 a          596 c          625 g          306 t
ORIGIN      
        1 ggaacacgat ctcgttgatc cactcgacct gaaccctcgg cacggactcc atcagatgcc
       61 cgtacgtgtc gagcgtaaac ccggccgagt agtgccccat ctgccgggcg atgtagagcg
      121 ggttcttccc ggccgcgatc aggagcgagg cgaaggtgtg gcgcaggtcg tagggccgga
      181 tcggccggac ctgtgcctgg gcgatcgcgt gcgcccacgc ccgcttcatg ttggtgcgag
      241 cgaacatccc accgccaggg ccggggaaca cgagcccgcc ggccgcacgg ctcgccagcg
      301 cctcgaggac gcggcgcacg gtgggcacca tgtccacgtc ccgcacggac cgatccgtct
      361 tcggcgcccc gagcaccttc gaccgggcct cgtatgacgc cgtgacgtgg atcttgttgg
      421 cggtgaagtc cggccagttc tgctccgtcc accgcatcgc ctggatctcc ccggggcgga
      481 gaccggcgaa cgtggcgacg agccacaccg gtcgccacgt ctcgggagcg acgagcagga
      541 accgtcgaag ttcggcaggc gtccacaacg gcaggggacg ccgcgggagc cgtaacttcc
      601 ggaccttccc gacaggcgag gcggccagat acccccagtc caccgctgcg ccgagcatct
      661 gcttgagcag ggcgagtgcg tggttcacgt gatcgtggag acggtggcgg ggctcaccgt
      721 ctcgcccagg aggggggcga gggccgcgcc gacccggcgg gtgctgaggc cgccgaggaa
      781 gacggcgcgg acgaggcgat tcacctgctc ggtgcgggcc tgataccggg ccacgacgga
      841 ggggcggaag ccggggtccc gggcccgggg gatctggaga tcggggatgg tgccccaggt
      901 ggtggtgagg cggcggcgat agacgccatt gcggtagccc gcgcggccat aatcgcggac
      961 gtaccggccg gcgttgaggt agtcggtgag ctcgtcatgc atgcgattct ccaagagcag
     1021 cttcaccgtc cggagcgtct gctgggagac atccccccag agggtgcgct cgtccttgac
     1081 ctcccgccac agatcggcta cggtgagatc ggtcagtggt tgcacactgg ccatgggcgt
     1141 atccctccct tgacttggtt gggcctaggt ttggggttgg ggatacgccc tctctcatca
     1201 gagccctgcc ttttacacaa agcactgtac actaccggat gcggaaggcc ggtggacatg
     1261 caacaggcgt tgcgatacca tgaatgcgca tcggttgtaa gccccacgag acccggcgcg
     1321 gattcagacg acaccgggtc tcgtgctgtc tagggctcta gggcgctacg gctccgcggg
     1381 gggctcgcgc ggcttgccgc tggactcgtt cctccgcgaa ccgctcggcc gcgcgcctct
     1441 ccacgaactt cttgacgtct gcctctcgga agagacgcac acgttgtcct tcgcccgcgg
     1501 tcgggattgc ccggagatcg ccccttcgtt ccagcgcgtg gatcgccgcc ggcgtaagat
     1561 tcagttgcct cgcaacatcg gataccgtga gcagcttctc catgggccct tcacctcgct
     1621 gcgtatttgt actatgctgc atgtaacata aagcaatcga ttccgcgctg cagactacga
     1681 cagctaccgg agccagaaat gacagggccc acagggcgcc tcaagaaagg ccgtcggacg
     1741 cgtacactgc gacagccgga agaccgcgat agaagcggtg ctcaggagga ctttcgcccg
     1801 ccacttgaga gactcaagta ctacagggtg atggcagggc cta
//
LOCUS       DASQGH010000331         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12856, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000331 DASQGH010000000
VERSION     DASQGH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12856"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..638
                     /locus_tag="VEW91_06445"
     CDS             198..638
                     /locus_tag="VEW91_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HYM91256.1"
                     /translation="MDPDPPTPGRPRVEAMLREARVARLATADARGQPHVIPVCFVVD
                     GQAIYTAIDEKPKRAAPRRLRRVRNIEANPRIALVVDHYEEDWRRLRFTLVTGTARIV
                     DGGPDHARAIALLREKYPQYRAMRLEDRPVLKISPRTVVTWAAG"
     gene            complement(649..1233)
                     /locus_tag="VEW91_06450"
     CDS             complement(649..1233)
                     /locus_tag="VEW91_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HYM91257.1"
                     /translation="MTTGSASMSVLDVIRSRRSVPKLKPDPVPSDLITELLDAAVWSP
                     NHKLTEPWKFFVLQGETKRRFGEVRRDFRRTQLPNPDAAEVQAALEKVYRDTVATPVI
                     IVVTSHVAGDAEQREEDFWATYGAAYAFMLGAWSRGLGTYFRTGGLRDYQPLRQLLGL
                     PADRRIIGVIYAGNPVEVPQRRRTRAAEKTVWLE"
     gene            complement(1284..>1502)
                     /locus_tag="VEW91_06455"
     CDS             complement(1284..>1502)
                     /locus_tag="VEW91_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYM91258.1"
                     /translation="AQAMATLVRYYGPQFMSRYDERALVFGPPEECARRAQAYLDAGV
                     QHLLLVPTTLEGGQVDRIAREVVPRLRA"
BASE COUNT          231 a          523 c          518 g          230 t
ORIGIN      
        1 agcgcggtgc tgagcgcgtt gagtgcggcc gggcgcctga gggtcagtgt ccagacacct
       61 tccaccccgt ccagggtcac gagctgctct gccatcgcct cgcctccttc ggatcgcatc
      121 gcccctgccg gtgatccaga ctcccaccgt catccccaat aggaaggatg ggacgaggga
      181 aagaagggaa cctcccgatg gatcctgatc ctccgacgcc aggacgcccg cgggtcgagg
      241 cgatgctccg cgaggcgcgg gtcgcccggc tcgccacggc cgatgcgcgc ggccagcccc
      301 acgtcatccc cgtctgcttc gtcgtcgacg ggcaggcgat ctacacggcg atcgacgaaa
      361 agcccaagcg ggccgcccct cgccgcctcc gccgggtccg caacatcgaa gcgaatcccc
      421 ggatcgctct ggtcgtcgat cactacgagg aggactggcg ccgcctgcgc ttcacgctcg
      481 tgaccgggac cgcccggatc gtcgatggag gcccggatca cgcgcgggcg atcgcgctcc
      541 tgcgcgagaa gtacccccag taccgcgcca tgcggttgga ggaccgtccg gtgctgaaga
      601 tctcgccgcg caccgtcgtc acgtgggcgg ccgggtgagg cgcggaggtc attccagcca
      661 gacggtcttc tcggcggcgc gggtccggcg ccgctggggc acctcaaccg gattgcctgc
      721 gtagatcacc ccgatgatcc ggcggtcggc cgggaggccg aggagctggc ggagcggctg
      781 gtagtcccga aggcctcccg tgcggaagta ggtgccgagg ccgcggctcc aggctccgag
      841 catgaacgca tacgcggcgc cgtaggtggc ccagaagtct tcttccctct gttccgcatc
      901 gcccgccacg tgcgaggtca ccacgatgat gaccggtgtg gcgaccgtgt cgcggtacac
      961 cttctcgagc gccgcctgca cctcggccgc gtccggattc ggcagctggg tccgccggaa
     1021 gtcgcgccgc acctcgccga accgccgttt ggtctctcct tgaagcacga aaaacttcca
     1081 tggctcggtc aacttgtggt tgggcgacca gaccgcggcg tcgagcagtt cggtgatgag
     1141 gtcggaggga accggatcgg gcttgagttt gggcacgctg cgccggcttc ggatcacatc
     1201 cagcacgctc atcgaggcag acccggtggt catggacatt cctcctcggg aaggtgatcg
     1261 gaagttcgag cgaccccgcg ccgctacgcg cgcagccggg ggacgacctc gcgggcgatc
     1321 cggtcgacct gtcccccctc gagcgtcgtc ggcacgagga gcaggtgctg gacgcccgcg
     1381 tccaggtacg cctgcgcgcg gcgcgcgcac tcctcggggg gaccgaagac cagcgcccgc
     1441 tcatcgtagc gcgacatgaa ctggggaccg tagtaccgga cgagggtggc catggcctgg
     1501 gc
//
LOCUS       DASQGH010000332         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24172, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000332 DASQGH010000000
VERSION     DASQGH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24172"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VEW91_06460"
     CDS             <1..513
                     /locus_tag="VEW91_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91259.1"
                     /translation="RKRIGIGYVPQEPSTFPQMTVEENLLVGGWTIRHNRGKLRDRLE
                     HIYALFPGLADRRRARATLLSGGQLRMLSVAKELVAMPLLLLVDEPTAGLAPRVAADV
                     YDLLVRSRELGITVLLVDQNISEAVAIADHVYLIVMGRVEREGPREVFVRDLRAIIQE
                     TLVGAAPRAS"
     gene            588..1817
                     /locus_tag="VEW91_06465"
     CDS             588..1817
                     /locus_tag="VEW91_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91260.1"
                     /translation="MLIATAMVMAVVVGGGGAPGWSAPAPITLGVLTPLSPPGDASAG
                     QLIVHGAELGVQYINTVMGGVFGGKDGCQLPAAQVKLAVEDDSGVPEKAIAGFRRLVS
                     EDHAVAVWGAFHSSAMLAAAPLADEFMVPYISTQASVADITRRHFAAVFRTHAIDPDR
                     AATWIGFIQAQGWHKVAMLAENTDYGIALVEATKALIAAKKLDVTLDAVIFDRTSADL
                     TPQLLKFKAARPDLVLNVGVGTPAYLIVKQAHDIGLFPQVAMLGSYDFPVRPDYWKNL
                     GPAGTSLTFISYYHPRMALTALGKWAALEHAKRYHDVAIYSNLNSFGDAVIVAQAMNQ
                     ACSSDPKRIIAALERGRFATWISDEVRFPRAEGVFWHQWSPPMIILQYTQNDQTYETA
                     TIVYPPAMKTGTYTAPR"
     gene            1828..2718
                     /locus_tag="VEW91_06470"
     CDS             1828..2718
                     /locus_tag="VEW91_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM91261.1"
                     /translation="MDATLLAQYTLAGLVIGGIYCLMAVGITFIYSIVKMINWAMGEF
                     YMIGGYLQYLLITTWLGPSRWYVALPLAVLGVGGLGMVIQRVLLQPMFVGQVERLDEY
                     ATIVTISLTVLLRNLAIVVFGPYQFSPRDYAPPVQLGPLPLNGSRWVAFLGAVILLGG
                     FAFVARWTWFGRALRAAAQNRLGALSAGINLPRVDMLAFGVGVGLAGAAGALLAPVFL
                     VFPESGALSTVKGFEIIVIGGLGSIPGSIVGGLLLGLVESLGSVFLSASFRDVYGFGL
                     LLLLLVLRPAGLWGERAREA"
     gene            2748..3713
                     /locus_tag="VEW91_06475"
     CDS             2748..3713
                     /locus_tag="VEW91_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM91262.1"
                     /translation="MARSLDRRPPWAAPGVRAGVAAVGLAAAALLPFLLGDYPIHVLT
                     VACYYVIMASSWNLIAGYTGLFSLAHHVFSGIGGYTSALLVIRSGLPIPFGIVAGGIA
                     AGGAGYVIGALTLRMRALYLALATWAFAESTRVFVSMEYGITRGDLGLPVPKLFPTIS
                     PRPYYELFLVLAAAAVAGLGLLLRSRIGYRLRAIRDDEGAARAAGINTVRWKRFVFAV
                     SSAAAGVAGAVYGHYIGLLSPVGMKFDEMALIIIMVILGGQRTLAGPVVGALVVELVS
                     EALRAYGQIRMVLFALVVLGMMRLYPSGLIGMARAAAGRVAAGRG"
     gene            3801..4124
                     /locus_tag="VEW91_06480"
     CDS             3801..4124
                     /locus_tag="VEW91_06480"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HYM91263.1"
                     /translation="MATEIVQLEEELASVRARLAEARRRAPEEEIRNYVFQGPGGKDV
                     ALSALFGDKPDLLVIHNMGKGCRYCTLWADGFNGVRDHLEDRAAFVVVSPDDPQDQAA
                     FAAER"
     gene            4137..4361
                     /locus_tag="VEW91_06485"
     CDS             4137..4361
                     /locus_tag="VEW91_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91264.1"
                     /translation="MVSARGTTFFKDMGFQREKGGAMPGVSAFRRGEDGRIYRVAHTR
                     FGPGDDFCSVWHLFALLADGAGEWEPKFHY"
     gene            4473..>4948
                     /locus_tag="VEW91_06490"
     CDS             4473..>4948
                     /locus_tag="VEW91_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM91265.1"
                     /translation="MQISTTARSITPSMTIGMDDRARRLRRQGVDVISFAAGEPDFDT
                     PEAIKRAGARAIQEGFTKYTSPAGIIELRQAVAARLEADYGARYAPEEVVITAGGKPA
                     LYFALLALCEPGDEVLVPIPAWVSYMEQVKLAGARPVPVPTEPREEFQLLPSRVAPL"
BASE COUNT          687 a         1635 c         1796 g          830 t
ORIGIN      
        1 cggaagcgga tcgggatcgg ctacgtcccc caggagccca gcacgttccc ccagatgacg
       61 gtcgaggaga accttctggt cggcgggtgg acgatccggc acaaccgggg aaagctgcgg
      121 gatcgcctcg agcacattta cgccctgttt ccgggactgg ccgatcgtcg ccgcgcccgg
      181 gcgacactcc tcagcggagg gcagctccgg atgctctcgg tcgcgaaaga actggtcgcg
      241 atgccgttgc tgctgttggt cgacgagccg acggccgggc tggcgccgcg cgtcgccgct
      301 gacgtctatg acctcctcgt ccgcagccgc gagttgggga tcacggtcct cttggtggac
      361 cagaatatct ccgaggcggt cgcaatcgcg gaccacgtct acctgatcgt catgggtcgc
      421 gtggagcggg aaggcccgcg cgaggtgttc gtgcgggatc tccgcgccat catccaggag
      481 acgctcgtcg gggccgcgcc ccgggcatcc tgacccgcgg catcgttcgc gaacgcgcgc
      541 aggtatcaga tggagatggg ggtgtgaaca ggatgggtag gggaccggtg ctgatcgcaa
      601 ccgcaatggt gatggcggtc gtcgtggggg gcggcggggc gcccgggtgg tccgcgcccg
      661 cgcccatcac gcttggggtg ctcaccccgc tctcccctcc gggcgatgcc tcggcgggac
      721 agctgatcgt ccacggcgcg gagctcggcg tccagtacat caacaccgtg atgggcggcg
      781 tcttcggcgg caaggacggg tgccagcttc ccgccgccca ggtcaagctc gccgtcgaag
      841 acgatagcgg cgtccccgag aaggcgatcg ccgggttcag gcggctggtc tccgaggacc
      901 acgcggtggc cgtgtggggc gcgttccaca gttccgcgat gctcgccgcc gcgccgctgg
      961 cggatgagtt catggtgccg tacatcagca cccaggcctc ggtcgccgac atcaccaggc
     1021 ggcacttcgc ggcggtgttc cggacccacg cgatcgaccc cgaccgcgcc gccacgtgga
     1081 tcgggttcat ccaggcccag gggtggcaca aggtcgcgat gctcgccgag aacaccgact
     1141 acggcattgc gctggtggag gccacgaaag cgctgatcgc ggccaagaaa ctcgacgtga
     1201 ccctggatgc ggtaatcttc gaccggacgt ccgccgacct gacgccccag ctcctgaagt
     1261 tcaaggcggc ccggccggac ctggtgctca acgtcggggt gggcaccccc gcgtacctga
     1321 tcgtcaagca ggcgcacgac atcgggctgt tcccgcaggt ggcgatgctc ggctcctacg
     1381 actttcccgt gcggcccgac tactggaaga acctcgggcc ggccgggacc tccctgacct
     1441 tcatctccta ctatcatccg cggatggcgc tcaccgcgct cggcaagtgg gcggccctcg
     1501 agcacgcaaa gcgataccac gacgtggcga tctactccaa cctgaattcc ttcggcgatg
     1561 cggtgatcgt ggcccaggcg atgaaccagg cgtgcagcag cgatcccaag cggatcatcg
     1621 cggccctcga gcggggacgg tttgcgacct ggatcagcga cgaggtgagg ttcccccgcg
     1681 cggagggggt cttctggcac cagtggtctc cgccgatgat catcctgcaa tacacgcaga
     1741 acgaccagac ctacgagacg gcgacgatcg tctatccgcc cgcgatgaag acagggacgt
     1801 atacggcccc gcgctagcgg cctgcgcgtg gacgccaccc tcctcgccca gtacaccctc
     1861 gccgggttgg tgatcggggg gatctactgc ctgatggcgg tcgggatcac cttcatctac
     1921 agcatcgtga agatgatcaa ctgggcgatg ggggagttct acatgatcgg tgggtacctg
     1981 cagtacctgc tgatcaccac ctggctcggg ccgtcccggt ggtatgtcgc gttgccgctg
     2041 gccgtcctcg gcgtgggggg gctcggcatg gtgatccagc gggtcctgct ccaaccgatg
     2101 ttcgtgggcc aggtggagcg gctggacgag tatgcgacga tcgtgacgat cagcctcacg
     2161 gtgctgctcc gcaacctcgc gatcgtcgtg ttcggcccgt atcagttttc gccgcgggac
     2221 tacgccccgc cggtgcagct gggtcccttg ccgctcaacg gcagccggtg ggtggcgttc
     2281 cttggggcgg tcatcctcct gggcgggttt gcgttcgtgg cccgctggac gtggttcggg
     2341 cgggccctgc gggcggcggc ccaaaatcgc ctcggcgcgc tctccgcggg gatcaacctg
     2401 ccgcgggtgg acatgctcgc cttcggcgtc ggcgtcggat tggccggggc ggcgggggcg
     2461 ctcttggccc cggtgttcct cgtgtttccc gagagcgggg ccttgagcac cgtcaagggc
     2521 ttcgagatca tcgtgatcgg gggcctgggc tcgatcccgg gcagcattgt cggcgggctg
     2581 ctgctcgggc tggtggagag cctgggctcg gtctttctgt cggcgtcgtt ccgcgacgtg
     2641 tacgggttcg ggctgctgtt gctgctgctc gtgctgcggc ccgccgggct ctggggcgag
     2701 cgggcccggg aggcctagcg acggctcggg ccacccccgc gggggcggtg gcccgctccc
     2761 tcgaccgccg gccaccctgg gcagcgcccg gggtgcgggc cggcgtcgcg gcggtcgggc
     2821 ttgcggcggc ggcgctcctt cccttcctgc tcggcgatta cccgatccac gtcctgaccg
     2881 tggcctgcta ctacgtcatc atggcctcga gctggaacct catcgccggg tacacaggcc
     2941 tgttctcgct cgcgcaccac gtcttcagcg gcatcggggg gtacacctcg gcgctgctcg
     3001 tgatccgctc cggcctcccg atcccattcg ggatcgtcgc gggggggatc gccgccggcg
     3061 gcgcggggta cgtgatcggc gccctgacgc tccggatgcg cgccctgtac ctggcgctgg
     3121 cgacgtgggc gttcgccgaa tcgacccgcg tcttcgtctc gatggagtac gggatcacgc
     3181 ggggcgatct gggactcccc gtccccaagc tcttccccac gatctcccct cggccgtact
     3241 acgagctgtt cctggtcctc gccgcggccg cggtcgcggg tctcgggctg ctgctccgct
     3301 ccaggatcgg gtaccggctg cgcgcgatcc gggacgatga aggcgcggcg cgggctgccg
     3361 gaatcaacac ggtgcggtgg aagcgcttcg tgttcgccgt gagcagcgcg gcggccgggg
     3421 tggccggcgc ggtgtacggg cactacatcg ggctcctgag ccccgtcggc atgaagttcg
     3481 acgagatggc gctcatcatc atcatggtga tcctcggcgg ccagcggacg ctcgcgggcc
     3541 cggtggtcgg cgccctcgtc gtggagctcg tctcggaagc gctgcgcgcc tacggccaga
     3601 tccggatggt cctgttcgcc ctcgtggtgc tggggatgat gcgcctgtat ccttccgggc
     3661 tgatcgggat ggcgcgggcg gccgcggggc gggtcgcggc gggccggggg tgacttgaca
     3721 gccggaaacc ggcagggata ggctgactgc caccggccgg agaccgggtg ggtccacccg
     3781 agcgagaggg ggagggtgag atggcgacgg aaatcgtcca gttggaggaa gagcttgcca
     3841 gcgtccgcgc aagactcgcc gaggcccgcc ggcgcgcccc ggaggaggag atccgcaact
     3901 atgtcttcca ggggccgggc gggaaggacg tggcgctctc ggccctcttc ggcgacaagc
     3961 cggacctcct tgtcatccac aacatgggga aggggtgccg gtactgcacg ctgtgggccg
     4021 acggcttcaa cggcgtgcgg gaccatctgg aggaccgcgc cgcctttgtg gtcgtctccc
     4081 ccgatgatcc gcaggatcag gccgcctttg cggccgagcg gtgatggaag ttcagaatgg
     4141 tgtcggctcg ggggaccacg ttcttcaagg acatggggtt ccagcgggag aagggcggcg
     4201 ccatgccggg cgtgtccgcg ttccgcaggg gcgaagatgg aaggatctac cgcgtcgcgc
     4261 acacccgctt cggccccggc gacgatttct gctcggtctg gcacctgttt gccctcctgg
     4321 ccgacggcgc cggggagtgg gaaccgaagt tccactactg acgggatgcg ccgcctccgc
     4381 ggcgaatacc agtctgcggc ggcgatggcc gcggcctgat gcgcgaccac gccgggcgtg
     4441 gctccggccg gcccggccca ggaggccaac cgatgcagat ctcgacgact gcccgctcga
     4501 tcaccccctc gatgacgatc gggatggacg atcgggcgcg ccggctgcgg cggcaggggg
     4561 tcgatgtcat cagttttgcg gcgggcgagc cggacttcga cacccccgag gccatcaagc
     4621 gggcgggggc cagggcgatc caggaagggt tcaccaagta caccagcccg gccggaatca
     4681 tcgagttgcg gcaggccgtc gccgcacggt tggaggccga ctacggggcg cggtacgccc
     4741 ccgaggaggt cgtgatcacg gccggcggca agccggccct gtacttcgcg ctgcttgcgc
     4801 tgtgcgagcc gggggacgag gtgctggtgc cgatccctgc gtgggtctcg tacatggagc
     4861 aggtgaaact tgcgggcgcg aggccggtgc cggtgcccac cgaaccccgg gaagagttcc
     4921 agctgctccc gtcccgggtc gcgccgct
//
LOCUS       DASQGH010000333         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1422, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000333 DASQGH010000000
VERSION     DASQGH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VEW91_06495"
     CDS             <1..277
                     /locus_tag="VEW91_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYM91266.1"
                     /translation="VCVECKRRGTVCVMVAHGTPCLGPVTHAGCGAICPAYHRGCYGC
                     YGPMETPNTGSLSAWWRKLGVADADIARAYRGTNAFAEAFRKAGERP"
     gene            274..1569
                     /locus_tag="VEW91_06500"
     CDS             274..1569
                     /locus_tag="VEW91_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HYM91267.1"
                     /translation="MTSRSRTIKVDELARVEGEGALYVKVKDGRVVDVRLKIFEPPRF
                     FEAFLRGRAFAEAPDITARICGICPVAYQMSACHAMEDACGVRVPGPLRALRRLLYCG
                     EWVQSHALHVYMLHAPDFLGYASAIHMAKDHPRIVERGLALKKAGNALMELLGGRAVH
                     PVNVRVGGFYRVPSRRELGAIVGTLEWARDAALDTVRWVAGFEFPEVEGDYEFVALRH
                     PSEYPLNEGRIVSSQGLDIAIHEYADHFVEEQVAHSNALRSRLRERGAYFVGPLARYN
                     LNFDRLAPPAQEAARRAGLGPVCRNPFRSIIVRAVEMVHACDEALRLIGNYEMPETPA
                     VPVPPRAATGHGCTEAPRGLLYHRYALDGDGAIAEAAIVPPTAQNQVTIEADLRRIVQ
                     ESLALSDDRLTWRCEQAVRNYDPCISCATHFLRLQIERE"
     gene            1578..>2029
                     /locus_tag="VEW91_06505"
     CDS             1578..>2029
                     /locus_tag="VEW91_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HYM91268.1"
                     /translation="MLVIGVGNPYRRDDAAGLIAARRLGAAAPGLVLVREESGEGAAL
                     LEAWEGADAVIVLDAVCSGAPPGTIRRIDAHRETIPTEVFHSSTHAFNVAEAIELARV
                     LRRLPRRLIVFGVEGAHFDAGLGLSPPVERAVERLVKCGLEELHHTGG"
BASE COUNT          283 a          704 c          744 g          298 t
ORIGIN      
        1 cgtgtgcgtg gaatgcaagc ggcgggggac cgtctgcgtc atggtcgccc acggcacccc
       61 ctgcctgggg ccggtcaccc acgccgggtg cggggccatc tgtcccgcct accaccgagg
      121 ctgttacggg tgctacggtc cgatggagac ccccaacacg ggctcgcttt cggcctggtg
      181 gcgcaagctc ggggtggcag acgcggacat tgcgcgcgcg taccgcggaa ccaacgcctt
      241 cgcggaggcg tttcggaaag ccggcgagcg gccgtgacga gccggagcag gacgatcaag
      301 gtggacgagc tggcccgcgt cgagggagag ggcgcgctct acgtgaaggt caaggacggc
      361 cgggtcgtcg acgtccgtct caagatcttc gagccgcccc gattcttcga ggcgttcttg
      421 cggggccgcg cctttgccga agcccccgac atcacggccc ggatctgcgg catctgcccg
      481 gtcgcctacc agatgagcgc ctgccatgcc atggaggatg cctgcggggt tcgcgtcccg
      541 gggccgctgc gcgcgttgcg ccggctcctc tactgcggcg agtgggtcca aagccacgcc
      601 ctccacgtgt acatgctcca cgcgcccgac ttcctggggt atgcgagcgc gatccacatg
      661 gccaaggatc acccccggat cgtggaacgc ggcctggcgt tgaagaaggc gggcaacgcg
      721 ctcatggagc tgttgggtgg ccgcgcggtg caccccgtca acgtgcgggt gggcggcttc
      781 taccgggtcc cgtcccggcg cgagttggga gcgatcgtcg gcacgctcga gtgggcccgg
      841 gacgcggccc tggacaccgt gcggtgggtg gccggcttcg agttccccga ggtcgagggg
      901 gactacgagt tcgtggcgct gcggcacccg agcgagtacc cgctcaatga gggccggatc
      961 gtgtccagtc aggggctcga tatcgccatc catgaatacg ccgatcactt cgtcgaggag
     1021 caggtcgcgc actcgaacgc gctgcgctcg cgcctgcggg agcggggggc gtacttcgtc
     1081 gggcccctcg cccgctacaa cctgaacttc gaccggctgg ctccgccggc gcaggaggcg
     1141 gcccggcgcg cgggcctcgg gccggtgtgc cgcaacccct tccggagcat catcgtgcgg
     1201 gcggtcgaga tggtccacgc gtgcgacgag gccctgcgcc tcatcggcaa ctacgagatg
     1261 ccggagaccc ccgcggtccc cgtgccgccg cgggcggcca cgggacacgg ctgcacggag
     1321 gcgccgcgcg gcctgctgta ccaccggtac gcgctggacg gggacggcgc gatcgcggag
     1381 gcggccatcg tgccccccac cgcgcagaac caggtgacga tcgaagccga ccttcgccgg
     1441 atcgtgcagg agtccctggc cttgtccgac gaccggctca cctggcgatg cgagcaggcg
     1501 gtccgcaact acgacccgtg catctcatgc gccacccact tcctccggct ccagatcgag
     1561 cgcgagtagc gccccgcgtg ctcgtcatcg gcgtggggaa tccctaccgg cgcgacgacg
     1621 cggcggggct gattgccgcg cggcggctgg gagccgccgc ccccggcctc gtgttggtgc
     1681 gcgaagagag cggggagggc gccgcgctgc tggaggcctg ggaaggggcc gacgcggtga
     1741 tcgtgctcga cgcggtctgc tcgggcgccc cgccggggac gatccgccgg atcgacgcgc
     1801 atcgggagac gatccccacc gaggtctttc actcgtccac gcacgccttc aacgtcgccg
     1861 aggcgatcga actggcccgg gtcctccggc ggctgccccg acgcctgatc gtgttcgggg
     1921 tggagggagc gcacttcgac gcgggcctcg gcctctctcc cccggtcgaa cgggcggtgg
     1981 agcgcctcgt gaaatgcggc ctcgaggaac tgcatcacac aggggggtg
//
LOCUS       DASQGH010000334         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46809, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000334 DASQGH010000000
VERSION     DASQGH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46809"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VEW91_06510"
     CDS             complement(<1..282)
                     /locus_tag="VEW91_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HYM91269.1"
                     /translation="MPRSPKNSVAAVGEFALIDRLRRLVPTSGPGVVLGVGDDAAVLQ
                     FPGPVVATCDVQVLGVHFTWELCGPEDVGWRAIAVNISDIAAMGGTPRFA"
     gene            complement(266..901)
                     /gene="thiE"
                     /locus_tag="VEW91_06515"
     CDS             complement(266..901)
                     /gene="thiE"
                     /locus_tag="VEW91_06515"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006883090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYM91270.1"
                     /translation="MTRGDWRVYVITDSQRARGRSPREIAQAAIEGGATAVQLRMKDE
                     PARAVLDAAREIGPLCRAAGVAFIVNDRVDIAQIAGADGVHIGQDDLPVRDVRGLLGP
                     GALIGVSAATVEEAVAAERAGADYLGVGAIYATGTKPDAGAPVGLDRLREIRRAVRIP
                     LVGIGGITADNAAAVIRAGASGVAVITAVTRAEDMPAAVRRIRGEVDAALP"
     gene            complement(898..1701)
                     /gene="thiM"
                     /locus_tag="VEW91_06520"
     CDS             complement(898..1701)
                     /gene="thiM"
                     /locus_tag="VEW91_06520"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HYM91271.1"
                     /translation="MVTAGRVADLLALVRERRPLIHHITNFVTAGDVANITLALGAAP
                     VMAQAPEEVEEVAAQAGALVLNTGTLTAQTAAAMLRAGQAANARGVPVVLDPVGAGGT
                     AFRTAWVKRILSEVRCACVRGNAGEIAALGGRPGAVRGVDALGVVGGLPDLARHLARG
                     TGAVIAATGIEDLVTDGATTLRIANGHPLLGRITGSGCMATAAVAAASAIEPEPLTAA
                     VAGLVWFEVAAERAAERAGGPGTFRAALADAVAALDRATIEDRARILAA"
     gene            complement(1695..1898)
                     /locus_tag="VEW91_06525"
     CDS             complement(1695..1898)
                     /locus_tag="VEW91_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HYM91272.1"
                     /translation="MRGALEELPGVLEVRVHLGTKDASIRFRPEQVTVEAMQRAVERV
                     DLRHAIRHRLLGWVGRLAGRRPW"
     gene            complement(1973..2494)
                     /gene="thiW"
                     /locus_tag="VEW91_06530"
     CDS             complement(1973..2494)
                     /gene="thiW"
                     /locus_tag="VEW91_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HYM91273.1"
                     /translation="MREGTDHERGQHLRRLVLAAMFAALGTLLGPLSIPVGPTRVAPL
                     QHTINAIAAIWLGPWYAVGAALVTASLRNLLHWGSLFAFPGSPFGALVVGYAYRMLRN
                     DAAALCEPIGTGPIGATVAALFFQPLVGSHATVWFFQVAFLASSIPGAILGYAVVRIL
                     RRVPAIGAWAAQP"
     gene            complement(2491..3159)
                     /gene="tenA"
                     /locus_tag="VEW91_06535"
     CDS             complement(2491..3159)
                     /gene="tenA"
                     /locus_tag="VEW91_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HYM91274.1"
                     /translation="MSLFARLEEAAGPIRERILSHPFVRGLGDGSLPEDRFQYYLRQD
                     YLFLIQYCRVLALAVARAEDLATAARFADLLNLTLNVEMELHRQYAWKAGIAPEVLED
                     TRPAPTTAAYTNHLRVAAEAGDLAAIVAAILPCAHGYWEIATALRTQEGWDRHPLYAD
                     WVRMYTSDEYEALARWVADLLDRLGEALPPDRETALREVFVQSQRYEYLFWEMAYRME
                     EWPV"
     gene            complement(3156..>4339)
                     /locus_tag="VEW91_06540"
     CDS             complement(3156..>4339)
                     /locus_tag="VEW91_06540"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF006370.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HYM91275.1"
                     /translation="FETPCPPGRNTEAIQRFLGDTLAGWGAEVRTVPLYPGDVEVIAR
                     LPGRGAGRSLLINGHVDVAQTTPGEAWTHPPFHPVRREGRIYGRGACDMKGGIASALS
                     ALRAVLETRGRPRGDVLVHLVTGEEMGEGGTQLALDHTPPVEFALVPEPSAGGLSLGQ
                     GGVVTGWITIQSPQTYHDAVRRRMIHAGGGVEGASAIEKMMKVLQALQDLERHWAVMK
                     SHPGFPPGTTTLNPAAIEGGRHPAFVADRCALWITVHFYPGETAEGVTAEIERTVLAA
                     SAADPWLRTHPPTFRWGGRSMIEDRGEIFPAFETDPGHPAIAIIERAHRAVHGALPPK
                     RMSTSVSDAGWLAARGIPTVVYGPGGWEQAHAIDEFVATADLLACARVYARVIAEWCG
                     A"
BASE COUNT          575 a         1667 c         1541 g          556 t
ORIGIN      
        1 ggcaaaccgc ggggtcccgc ccatcgcggc gatgtcgctg atgttgaccg cgatcgcgcg
       61 ccacccgacg tcctcggggc cgcacagttc ccaggtgaag tgcaccccca ggacctgcac
      121 atcgcaggtg gccacgaccg ggcccgggaa ctggagcacg gccgcgtcgt cgcccacgcc
      181 gagcaccacg cccgggccgc tggtcggcac gaggcggcgg agccggtcga tcagcgcgaa
      241 ctcgccgacc gcggcgacgg agttcttagg ggagcgcggc atcgacctcc ccccggatcc
      301 ggcggaccgc agccggcatg tcctcggcgc gtgtgaccgc ggtgatcacc gcgaccccgc
      361 tggctccggc gcggatgacc gccgcggcgt tgtcggccgt gatcccgccg atccccacga
      421 gcgggatgcg gaccgcgcgc cggatctccc gcaggcggtc cagtcccaca ggcgcgcccg
      481 cgtcgggctt ggtgccggtg gcgtagatcg cccccacgcc gaggtaatcg gcccccgcgc
      541 gctcggcggc gacggcctct tcgacggtgg cggcggagac gccgatgagg gcgccgggcc
      601 ccaagagccc gcggacgtcg cggaccggca ggtcgtcctg gccgatgtgg acgccgtcgg
      661 ctccggcgat ctgcgcgatg tcgacccggt cattgacgat gaaggcgacg ccggccgcgc
      721 ggcacagcgg cccgatctcg cgcgcggcgt cgaggacggc ccgcgccggc tcgtctttca
      781 tccgcagctg cacggcggtt gccccgccct cgatcgccgc ctgggcgatc tcgcgggggg
      841 agcgcccccg cgcccgttgg gagtcggtga tgacgtacac cctccagtcc ccccgcgtca
      901 tgcggccagg atccgcgccc ggtcctcgat cgtcgcgcgg tcgagcgcgg ccacggcgtc
      961 ggcgagcgcc gcgcgaaacg tgccgggccc cccggcccgc tcggcggcgc gctcggccgc
     1021 gacttcgaac cagacgagcc cggcgacggc cgcggtgagg ggctccggct cgatggcgct
     1081 ggcggcggcc acggccgccg tggccatgca tccgctcccc gtgatccggc cgaggagcgg
     1141 atggccgttc gcgatgcgca gcgtggtggc gccgtcggtc accagatctt cgatccccgt
     1201 cgcggcgatc accgcgcctg tgccccgggc caggtgccgg gccaggtcgg ggagcccccc
     1261 gaccacgccc agggcatcga cgccccggac cgcgccgggc cgccccccga gggccgcgat
     1321 ctcccccgcg ttgccgcgga cgcacgcgca ccggacttcg ctgaggatcc gcttcaccca
     1381 cgcggtcctg aacgccgtgc cccccgcgcc gaccggatcg aggacgaccg gaacgccgcg
     1441 cgcgttcgcc gcctgaccgg cccggagcat cgccgcggcg gtctgggccg tcagcgtgcc
     1501 ggtgttgagg accagggcac cggcctgggc ggcgacctcc tccacctcct cgggggcctg
     1561 cgccatcacc ggcgcggcgc ccagcgcgag cgtgatgttc gccacgtccc ccgcggtcac
     1621 gaagttggtg atgtggtgga tgagcgggcg gcgttcgcgt accagcgcca ggaggtccgc
     1681 cacccgcccg gccgttacca cggccggcgc cccgcgagcc ggccgaccca cccgaggagc
     1741 cggtgccgta tcgcgtgtcg taggtccacg cgctccacgg cgcgctgcat cgcctccacc
     1801 gtcacctgct ccggccggaa cctgatggag gcgtccttgg tccccaggtg cactcggacc
     1861 tccaggaccc cggggagctc ctccagagcc cctcgcaccc tccgcactca gagcccgcag
     1921 aggatgcggt ccacccggag gaccgcggtg gcctcccggg gtgccccgcc ggtcatggct
     1981 gcgcggccca cgcgccgatc gccgggacgc gccgcaagat ccggaccacg gcgtacccga
     2041 ggatggcgcc cggaatgctg ctcgccagga acgccacctg gaagaaccac acggtggcgt
     2101 gggatcccac gagcggctgg aagaacaacg ccgcgaccgt ggcgccgatg ggaccggtgc
     2161 cgatcggctc gcacagtgcc gccgcgtcgt ttcgcagcat gcgatacgcg tagccgacca
     2221 cgagcgcccc gaacgggctc ccggggaagg cgaacagcga gccccagtgc aggaggttcc
     2281 gcaacgatgc ggtcaccagg gccgcgccca ccgcgtacca ggggccgagc cagatagccg
     2341 cgatcgcgtt gatcgtgtgc tggagcggcg cgaccctggt cgggcccacc gggatgctca
     2401 gcggcccgag cagcgtcccg agggccgcaa acatcgccgc cagcaccagc cgcctgagat
     2461 gctggccgcg ctcgtgatcc gtcccctccc tcataccggc cactcctcca tccgatacgc
     2521 catctcccag aacaggtact cgtagcgctg gctctgcacg aacacctcgc ggagcgccgt
     2581 ctcgcggtcg ggggggagcg cctctccgag gcgatccagg aggtcggcca cccaccgggc
     2641 cagcgcttcg tattcgtcgg acgtgtacat ccggacccag tcggcgtaga gcgggtggcg
     2701 gtcccagccc tcctgcgtgc ggagggcggt cgcgatctcc cagtagccgt gggcgcacgg
     2761 caggatcgcc gccacgatcg cggccagatc cccggcctcg gcggccaccc gcaggtggtt
     2821 cgtgtacgcg gcggtggtgg gggcgggccg ggtgtcctcc aggacctcgg gggcgatccc
     2881 cgccttccac gcgtactggc ggtgcaactc catctcgacg ttgagcgtga ggttgagcag
     2941 gtcggcgaac cgggcggcgg tggccaggtc ctcggcccgc gcgaccgcca gggcgaggac
     3001 gcggcagtac tggatcagga acaggtagtc ctgccgcagg tagtattgaa accggtcctc
     3061 ggggagcgat ccgtctccca gcccacgcac gaacgggtga ctgaggatcc gctcgcggat
     3121 cggcccggcg gcctcctcca gccgcgcgaa caggctcatg cgccgcacca ctccgcgatc
     3181 acccgggcgt acacgcgcgc gcaggccagc aggtccgccg tggcgacgaa ctcgtcgatc
     3241 gcgtgggcct gctcccaccc gcccggcccg tagacgaccg tcgggatgcc ccgcgcggcc
     3301 agccacccgg cgtcgctcac cgacgtggac atccgcttgg gcgggagcgc cccgtgcacc
     3361 gcgcggtggg cgcgctcgat gatggcgatg gccggatggc ccgggtcggt ctcgaacgcg
     3421 gggaagattt ctccccggtc ctcgatcatc gaccggcccc cccaccggaa cgtcggcggg
     3481 tgggtgcgca gccaggggtc tgcggccgag gcggccagga ccgtgcgctc gatctccgcc
     3541 gtgaccccct cggctgtctc ccccggatag aagtgcacgg tgatccacag cgcgcaccgg
     3601 tcggcgacaa aggccgggtg ccggcccccc tcgatcgccg ccgggttgag cgtggtcgtc
     3661 cccgggggga acccggggtg ggatttcatc accgcccaat ggcgctcgag gtcctggagg
     3721 gcctggagca ccttcatcat tttttcgatc gcgctcgccc cctcgacccc gccgcccgca
     3781 tggatcatcc gccggcgcac ggcgtcgtgg taggtctggg ggctctggat cgtgatccac
     3841 cccgtcacga cgcccccctg gccgagcgag aggcccccgg cagagggctc ggggaccagc
     3901 gcgaactcga ccggcggggt gtggtccagc gcgagttgcg tgcccccctc ccccatctcc
     3961 tcccccgtca ccaggtgcac gagcacgtcc ccgcgggggc gcccccgggt ctcgaggacg
     4021 gcccggagcg ccgagagcgc tgaggcgatc ccgcccttca tgtcgcaggc gccccgtccg
     4081 tagatgcggc cctcccggcg gaccgggtga aacggcggat gggtccacgc ttcccccggg
     4141 gtggtttggg ccacgtcgac gtggccgttg atcaggagcg accgcccggc tccccgcccc
     4201 gggaggcgcg cgatgacctc gacgtcgccc gggtacagcg ggaccgtgcg cacctccgcc
     4261 ccccaccccg cgagcgtgtc gcccaagaac cgctggatcg cctcggtgtt gcgcccaggc
     4321 gggcacggcg tttcaaacg
//
LOCUS       DASQGH010000335         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49579, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000335 DASQGH010000000
VERSION     DASQGH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49579"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VEW91_06545"
     CDS             <1..234
                     /locus_tag="VEW91_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91276.1"
                     /translation="DEPSMGLAPVLVELIFRTIQEINARGVTILLVEQNALMALQIAK
                     RGYVLETGRIVMTDRADALRANDLVRKSYLGEL"
     gene            421..1668
                     /locus_tag="VEW91_06550"
     CDS             421..1668
                     /locus_tag="VEW91_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYM91277.1"
                     /translation="MSLVLSGLLATGTYIVPSAPAATMGPVTDPIGVVQVGPGQPITI
                     AYWLVTSGPDGALGTDSKHGIEIAIDDVKGKLLGHDIKLIGEDSGCNAEGGQTAATKI
                     AANTAVVAAIGSSCSSEAVPGAPILWKAGIVTVSPSNTAPRLTDPARGPDFDGYLRVA
                     HNDKIQGAVAATFARKVLKVAKAASIHDGSPYAEGLANEFAANFNRLGGKMTSQEAVA
                     PTDTDMKPVLTRIASGKPDLIYYPIFIAAGGFVTRQAREIEGLKNTKLMGADGLLSPD
                     FAKAAGDSAKGMYLSSPDLTPEALGPKYSKQFLPKYEKMFGQTPTAAFHPHSYDAAMI
                     LFAAIQKVAKSQGGSLYIGRKALRDALFATKGFKGLTGTITCTKYGDCADPHIAVYQV
                     VSADPATWEPGKDPKKIYPIGKP"
     gene            1770..>2354
                     /locus_tag="VEW91_06555"
     CDS             1770..>2354
                     /locus_tag="VEW91_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM91278.1"
                     /translation="MPWSLIRRVSFVDLFLWAFRIGVVIVVVVGSVATLVVGRYTGRQ
                     WADFVEFGLAQGAIYALIALGYTMVYGVLNMINFAHGEVFMSGAYTAYFVAAALAASG
                     FLSRYVLPSLLLVLLVSMAVSTGVALLLERIAYRPLRRAPRLIPLITAIGASFFLQYT
                     FRGLYGAGIQSYPDIAELKGEWQLGPLHSLRTQAI"
BASE COUNT          420 a          741 c          795 g          398 t
ORIGIN      
        1 gacgagccct cgatggggct ggccccggtg ctcgtcgaac tgatcttccg aacgatccag
       61 gagatcaacg cgcggggggt gacgatcctc ctggtcgagc agaacgcgct catggccctc
      121 cagatcgcga aacgaggata cgtgctggaa acgggccgaa ttgtgatgac cgaccgagcc
      181 gatgcgctcc gggcgaacga tctggtccgg aagagctatc tgggcgagct gtaggctggc
      241 ggaacgcgac cttcggcaag ggaacttctc aggccgtcaa gaacgtaaga ctgtgcgagc
      301 acaggatgtg tgcactgggg gacaaagggg gtgagcactg tccccgagga tggcgtgaag
      361 ttgatagcga catgaacagg cttcgctcag gagggtaaaa tgacccgaag gaagatattg
      421 gtgtcactcg tgctctccgg gctccttgcg acgggcacgt atatcgtgcc ctctgcgccc
      481 gccgcgacca tgggcccggt caccgacccg atcggcgtcg tgcaggtcgg gcccggccaa
      541 ccgatcacga tcgcctactg gctggtgacc tcgggccccg acggcgcgtt gggcacggac
      601 agcaagcacg gcatcgagat cgccatcgac gacgtgaagg gcaagctgct cgggcacgac
      661 atcaagctca tcggcgagga cagcggctgc aacgccgagg gcgggcagac cgccgcgacc
      721 aagatcgcgg ccaacacggc ggtggtggcg gcgatcgggt ccagctgctc gagcgaggcc
      781 gtgccggggg cgccgatcct ttggaaggcc gggatcgtga cggtgtcccc gtcgaacacg
      841 gcccccaggc tgaccgatcc ggcgcgcggg ccggattttg acggctacct ccgggtcgcg
      901 cacaacgaca agatccaggg ggcggttgcg gccacgttcg cccggaaggt cctgaaggtt
      961 gccaaggcgg cgagcatcca cgacggcagc ccgtacgccg agggactcgc gaacgagttc
     1021 gccgcgaact tcaacaggct gggagggaaa atgaccagcc aggaggcggt tgcgccgacc
     1081 gacacggaca tgaagccggt gctcacccgg atcgcgtccg gcaagcccga tctgatctac
     1141 tacccgatct tcatcgccgc gggcgggttc gtgacgcggc aggcgcggga gatcgagggc
     1201 ctgaagaaca cgaagctgat gggcgcggac ggcctgctct cacccgactt cgccaaggcg
     1261 gccggcgact cggccaaggg catgtacctc agcagcccgg atctgacgcc ggaagcgctg
     1321 ggcccgaagt atagtaagca gttcctgccg aagtacgaga agatgttcgg ccagacgcct
     1381 acagccgcat tccaccccca ctcctacgat gctgcgatga ttctcttcgc cgcgatccag
     1441 aaggtcgcca agtcgcaggg ggggtcgctg tacatcgggc ggaaggccct gcgggacgcc
     1501 ctcttcgcca ccaaggggtt caagggcctt acggggacca tcacatgcac caagtacggc
     1561 gattgtgccg atccgcacat cgccgtatac caggttgtca gcgcggatcc cgcgacctgg
     1621 gagccgggga aggatccgaa gaaaatttac ccgattggga agccctaaac gatcgctccg
     1681 gttgacgagg tgatctgggg gatgaatcgg aggcgggctg cgtcttcggt tcatcccccg
     1741 atgctttctc cgtcttgcgg gggtgcgcga tgccctggag cctgatccgc cgcgtgtcct
     1801 ttgtggatct gttcctctgg gcgttccgca tcggcgtcgt catcgtggtc gtcgtcgggt
     1861 ccgtggcgac cctggtggtg gggcggtaca cgggcaggca gtgggcggat ttcgtcgagt
     1921 ttggcctcgc ccagggggcc atctacgccc tgatcgccct cggctatacg atggtctacg
     1981 gcgtcctcaa catgatcaac ttcgcccacg gcgaggtctt catgagcggg gcctataccg
     2041 cctacttcgt ggccgcggcg ctcgccgcct cggggttcct gagccggtac gtcctgccga
     2101 gccttctgct ggtgcttctg gtgtcgatgg cggtatcgac aggggtggcc ctcttgctcg
     2161 aacggatcgc ctaccgcccg ctcaggcggg cgccccggct catccccctg atcacggcga
     2221 tcggcgcgtc gtttttcctc caatatacct tccgcggcct ctacggggcg ggaatccaat
     2281 cctaccccga catcgccgag ttgaaggggg agtggcagct cgggccgctc cacagcctca
     2341 gaacccaggc catc
//
LOCUS       DASQGH010000336         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7186, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000336 DASQGH010000000
VERSION     DASQGH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7186"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..101)
                     /locus_tag="VEW91_06560"
     tRNA            complement(25..101)
                     /locus_tag="VEW91_06560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Pro,seq:tgg)
     gene            248..418
                     /locus_tag="VEW91_06565"
     CDS             248..418
                     /locus_tag="VEW91_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91279.1"
                     /translation="MNEKMRSCFTPHIMMHSLFGLGLGALLVTVIPGLRSVWLALVVM
                     AIAVVLDATRKA"
     gene            456..2114
                     /locus_tag="VEW91_06570"
     CDS             456..2114
                     /locus_tag="VEW91_06570"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYM91280.1"
                     /translation="MADHRPALSASLARLTEAYLAALFDTYPTAAAALGLHEYDGRLQ
                     DSREPARARRIGGLRGFRAELAALPRGSLDPDDAHDHTLLGLAIDEELFELEDLREFE
                     RNPLAFSGPLDLSGYIKRNYAPLAERVRALTEHLRQIPDYLEGAAETLREPVPWPFLE
                     TALEVYGGTIQFQEQDLARAVRAVGNGPLWDGFERANHAALAALHRFVQHLETEVRPN
                     AVEAFAIGERRFRTMLRAGEMVDLPIERLLEIGRDDLARNRAGLLDAAARVAPGTPVR
                     EVLTRQAQDHPAARDLIPVGARLLDELREFVEAHGLVSIPSAVRCRVEETPPFARWAF
                     AMMDTAGPFEKAVESFYYITPPDPGWTPEQTEQWLRKFDYATLKDVGIHEVYPGHYVH
                     FLHMKQVPRPLRRVLTSYSFVEGWAHYCEEMMLDAGYGGDDSQLRLAQLSEALLRNVR
                     FVTSIEMHTRGLSQDGAARMFIEEAHLDPLPAEREAVRGTFDPGYLNYTLGKVMIRKL
                     RDDLQRAEGGQFSLRRFHDRLLALGAPPVPLARQRFLPDAPLTL"
     gene            complement(2121..2783)
                     /locus_tag="VEW91_06575"
     CDS             complement(2121..2783)
                     /locus_tag="VEW91_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91281.1"
                     /translation="MFAILVIWGFSIAVAMADLKDDMLAKIRKQVGRARLRPVDPLAG
                     RSVAETLAPTAMDDRERWVFHLVSGIAIALRLHVRILVLQDSMEHYVVHFGLGKRLTT
                     YRVDKAWVADAMAGKADQRERIHKAVEQYLRQEFLGGQIAAKPAPAPPAAPAAGGTAA
                     TPKPTAAAAAPGSPGAAPPAQPGLSREEIIAAARAKAEAMRAQRAAGGSPPQPPKDPA
                     QS"
     gene            complement(2859..3479)
                     /gene="rdgB"
                     /locus_tag="VEW91_06580"
     CDS             complement(2859..3479)
                     /gene="rdgB"
                     /locus_tag="VEW91_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HYM91282.1"
                     /translation="MLATRNSGKIRELMAIYDHLNVAFHSLADYPGVGDVAEGGATYA
                     ENAAAKARTVAAATGLPALADDSGVEIDALGGAPGIHSARFLGADATDAERNARILAL
                     LEGVPEAQRTARYRASVAVALPDGSVRVFDGVCEGRIARAGRGAGGFGYDPIFVPAGE
                     TRTMAEVPADVKNRISHRARALRAAEPYVTEVLRRSAGGSGGRSAK"
     gene            complement(3506..4240)
                     /gene="rph"
                     /locus_tag="VEW91_06585"
     CDS             complement(3506..4240)
                     /gene="rph"
                     /locus_tag="VEW91_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYM91283.1"
                     /translation="MQRSDGRRPDELRPLVHRRRYTKFAEGSVLIELGDTRVVCTASV
                     EERVPPWLRGTGQGWLTAEYGMLPRATQERSPRDTSTGKVGGRVQEIQRLIGRSLRVV
                     VDLERLGERTIWVDCDVLQADGGTRTASITGAFVAVMDALTLLKRTGAVPKLPVREFL
                     AATSVGLIEGVPILDLTFAEDSRARVDMNIVMTESGKFVEIQGTGEGAAFTKAEASAL
                     IALAERGIVTLIAKQRETLADILTTP"
BASE COUNT          665 a         1608 c         1494 g          705 t
ORIGIN      
        1 aaccagaccc ggcaggccag aaactggtcg gggcgcccgg acttgcaccg gggaccttct
       61 gctcccaaag cagacgcgct accaagctgc gccacgcccc gattttcaag gctttcagaa
      121 tggtagtgta gaccttccac cacgggccgt caacgccggc caacggcaag aggggaggga
      181 aaatggggcg cgtggataag ggcatttagg tgtgacgttc agggctgcat tttgggaggg
      241 ggactcgatg aacgagaaaa tgaggtcctg cttcacgccc cacatcatga tgcatagcct
      301 gttcggcctg ggactcggcg cgttgcttgt gacggtcatc ccggggctca gaagcgtctg
      361 gctcgccttg gtggtgatgg cgatcgcggt cgtgctcgac gccacgcgca aggcctaggc
      421 gcgggctttc tcctccctct cccgggggcc ccggcgtggc cgaccatcgc cccgcgctct
      481 ccgcgtccct tgctcggctg accgaggcat atctggccgc cctgttcgac acgtacccga
      541 cggcggcggc ggcattgggg ctgcacgagt acgatggccg cctgcaggac tctcgcgagc
      601 ccgcccgggc ccggcggatc ggtgggctcc ggggctttcg cgcagagttg gcggcgcttc
      661 cccgagggtc actcgatccc gacgatgcgc atgaccatac cctgctaggg ctcgcgatcg
      721 acgaggaact gttcgaactg gaggacctgc gggagttcga acgcaatccg ctggcgttct
      781 ccggtccgct cgatctcagc ggctacatca agcggaatta cgcccccctg gccgagcgcg
      841 tgagggcgct caccgagcac ctccggcaga tccccgacta ccttgagggg gctgcggaga
      901 cgctgcgtga gcctgtgccc tggccctttc tcgaaaccgc gctcgaggtg tatggcggga
      961 ccatccagtt ccaggagcag gacctggcgc gcgcggtccg ggcggttggg aacggtcccc
     1021 tctgggatgg cttcgaacgc gccaaccacg cggccctcgc cgcgctccac cgattcgtgc
     1081 agcacctgga gaccgaggtc cgcccgaacg ccgtcgaggc gttcgccatc ggcgagcggc
     1141 gcttccggac catgctgcgg gcaggcgaga tggtcgacct tccgatcgag cgtctgctcg
     1201 aaatcggccg cgacgatctg gcccgcaatc gggcggggct cctggatgcg gccgcgcggg
     1261 tcgcccccgg cacccccgtc cgcgaggtgc tcacccgcca ggcgcaggat caccccgcgg
     1321 cccgggatct catccccgtc ggcgcgcggc tccttgatga actccgggag tttgtcgagg
     1381 cgcacggcct cgtctccatc ccgtcggccg tcaggtgccg ggtcgaggaa acgcccccct
     1441 tcgcccggtg ggccttcgcc atgatggaca ccgcagggcc gttcgagaaa gccgtcgagt
     1501 cgttctacta catcaccccg cccgaccccg gatggacgcc cgagcagacg gagcagtggc
     1561 tgcgcaaatt cgactacgcc acgctcaagg atgtcggcat ccacgaggtc tacccggggc
     1621 actacgtcca cttcctgcat atgaagcagg tcccgcggcc actcaggcgc gtgctgacga
     1681 gctattcgtt tgtcgaaggt tgggcccact actgcgaaga gatgatgctg gacgccggat
     1741 acggggggga tgattcccaa ctccggttgg cgcagctgtc cgaggcgctg ctccgaaacg
     1801 tccggttcgt gacctccatc gagatgcaca cccggggcct ctcgcaggac ggcgccgcgc
     1861 ggatgttcat cgaggaggcc cacctggacc ccctccccgc cgaacgcgag gcggtgcggg
     1921 gcaccttcga ccccgggtac ctgaactata ccttggggaa agtgatgatc cggaaactcc
     1981 gcgacgatct ccagcgggcg gaaggcgggc aattttccct ccggcggttc cacgaccggc
     2041 tgttggcgct tggcgccccc ccggtccccc tcgcccggca gcggttcctg ccggacgcgc
     2101 ccctaacgct gtagcggagg tcaggactgg gccggatcct tgggcggctg cgggggcgat
     2161 ccaccggcgg cccgctgcgc ccgcatcgcc tccgccttcg cccgcgcggc cgcgattatt
     2221 tcctcgcggc tcaatcccgg ctgagcggga ggagccgctc ccggggaccc cggcgcggca
     2281 gccgcagcgg tgggcttcgg cgtggccgcg gtcccgccgg ctgcaggagc ggcgggtggt
     2341 gccggagcag gcttcgcggc gatctgtccc ccgaggaact cctgacgcag gtattgctcg
     2401 acggctttgt ggatccgctc gcgctgatct gccttgcccg ccatggcgtc tgccacccag
     2461 gccttgtcca cccggtaggt cgtgagccgc ttgcctagac cgaagtggac cacgtagtgc
     2521 tccatggagt cttgcaggac caggatccgc acgtgcagcc ggagcgcgat cgcgatcccg
     2581 ctgaccaggt ggaacaccca ccgctcccga tcgtccatcg ccgtcggcgc gagcgtctcc
     2641 gccaccgacc gcccggcgag cgggtccacg gggcgaagcc gggcccggcc gacctgcttt
     2701 cggatcttcg ccagcatgtc gtccttgaga tccgccatcg ccacggcgat ggagaacccc
     2761 cagatgacaa ggatcgcaaa caacagcgac cagtaggtga gaaacttgct cagggtgacc
     2821 cagctccttt ccgcacgccc gaggaatccc gcccgcggtt atttggcgct ccgtcccccc
     2881 gatcctcccg cggagcgccg gaggacctcg gtcacatatg gctcagcggc ccggagcgcg
     2941 cgcgcccggt ggctgatgcg gttcttgaca tcggccggaa cctccgccat cgtccgggtc
     3001 tctccggccg gcacaaagat cgggtcgtac ccgaacccac cggcgccgcg accggcccgg
     3061 gcgatccgcc cctcgcacac gccgtcgaag acccggaccg atccatcggg aagggccacg
     3121 gcgacgctcg cccggtaccg cgccgttcgc tgcgcctccg ggacgccctc gagcaacgcc
     3181 aggatgcggg cgttgcgctc cgcatcggtc gcgtccgcgc ccaagaagcg cgcggagtga
     3241 atgcccgggg cccccccgag cgcgtcgatc tcgacccccg agtcgtcggc gagcgccggc
     3301 aggcccgtcg cggcggcgac ggtgcgcgcc ttggccgccg cgttctccgc ataggtcgcg
     3361 cccccctccg ccacatcgcc gaccccggga tagtcggcca gcgagtggaa ggcgacgttc
     3421 aggtgatcgt agatggccat cagttcgcgg atcttaccgg agtttcgggt cgccagcacg
     3481 aggcggggtc cggcaacgcc gcccctcacg gggtggtgag gatgtcggcc aaggtctccc
     3541 gctgcttggc gatcagggtg acgatgcccc gctcggccag ggcgatgagc gcgctggctt
     3601 ccgccttggt aaacgccgcg ccttcccccg tgccctggat ctcgacgaac ttcccggact
     3661 cggtcatgac gatgttcatg tccaccctgg cgcgcgagtc ctcggcgaag gtgagatcca
     3721 ggatcggaac cccctcgatc agcccgacgc tcgtcgcggc gaggaactcc cggaccggga
     3781 gcttgggcac cgccccggtg cgcttgagga gcgtcagggc atccatgacg gcgacgaacg
     3841 cgccggtgat cgaggcggtc cgcgtgccac cgtccgcctg aagcacgtcg caatcgaccc
     3901 agatggtccg ctcgcccaac cgctcgagat cgacgaccac gcgcagggac cgaccgatga
     3961 ggcgctggat ctcctggacc cggccaccga ccttgccggt tgaggtgtcg cgcgggctgc
     4021 gctcctgcgt cgctcggggc agcatcccgt actcggccgt cagccatccc tgtcctgtgc
     4081 cgcgcaacca cggaggaacg cgctcctcga cgctggcggt gcagacgacg cgggtatccc
     4141 cgagttcgat caagaccgag ccttccgcga acttggtgta ccggcgccga tggacgagag
     4201 gtcggagctc atccggccgg cggccgtcgc tgcgttgcac ccgtggggaa ccccttcctg
     4261 acgatcccgt cctgccttgg gggccgcccg aaggacggcc ccctgtgcgc caccccgact
     4321 acgagaacgc tgggcctacc ggccccggcc gggaccctgg gccttgcgcc cggtctgcgc
     4381 gcccgcggct ccggcgcgcg cccgctcccg gtggccgttg agcacgaagc gctggtccag
     4441 cgccttgagg cgctccatga tgccgccgta tt
//
LOCUS       DASQGH010000337         4155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1428, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000337 DASQGH010000000
VERSION     DASQGH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEW91_06590"
     CDS             <1..800
                     /locus_tag="VEW91_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010170942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91284.1"
                     /translation="NARIGFSLIVLFTLIAVFAPRIAPEGFDDENLPGRLHPPTAQHW
                     IGTDELGRDVFSRVIYGSRISMMVGFVAVAGALTTGSLLGITAGYVGGRVDGLIMRIM
                     DVMLAFPSIILAIGIVAVRGPGLNNTILAVSIVDIPVYARVARASTLSIKEHEYVTAA
                     RAMGARAWRILMRAILPNAASPLVVQATLGVAGAILNAAALGFLGLGAQPPTPEWGAM
                     LSDSYKYLLTAFWAALAPGVAIAAMVLSFNLAGDGLHDALEPRLSES"
     gene            complement(797..2275)
                     /gene="hemG"
                     /locus_tag="VEW91_06595"
     CDS             complement(797..2275)
                     /gene="hemG"
                     /locus_tag="VEW91_06595"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HYM91285.1"
                     /translation="MIAENVLAKPQPLPSRAELLRVVVVGGGIAGLAAAHRLVELGRA
                     RRPPVELVLVEARDRLGGVVATERADGYLIEEGPDSFLTEKPWGLSLCDRLGLTGGLV
                     PTREGSRRTFIVCRGRLVPLPDGFLVLAPSRLGPILRSPLLSWRGKLRLAMDLVLPKR
                     QAGGDESVGAFVTRRFGREVLDRVVQPLVSGIYGADPFDVSLAAAMPRFVEMERQHRS
                     IIRALRRAQDRRPAVEESGPRWSLFVTLAGGMGELVSAAARRLPPASVKLRRRVTSLA
                     RSRGPRPWRVNLADGTALLADGVILAIPAPRAARLVAGLDPDLGRALAAISYASSAIV
                     TLAYRREEIRHALDGFGFVVPHAEDRQILGCTFSSLKFPGRAPDGVVLLRVFLGGARA
                     RELLDRDDAALGAAAQQELEPLLGISGSPHLVRVHRHVAAMPQYQVGHAERVAAIEGG
                     VARHPGLAVAGNALGGVGIPDCIHSGERAAERVLAQAQGQAS"
     gene            complement(2272..3198)
                     /gene="hemH"
                     /locus_tag="VEW91_06600"
     CDS             complement(2272..3198)
                     /gene="hemH"
                     /locus_tag="VEW91_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYM91286.1"
                     /translation="MPTYDAVLLIAFGGPDRMEDVRPFLANVVRGRRVPPERIDEVVH
                     HYARFGGHSPLTELTHRQARALEADLAARGSKIPVYVGMRNWTPYLHETLACMRAEGV
                     RRAVGIVMAPHHSYSSWDQYRENVAEARAQVGEGAPEVSYLRAWFDHPGFIEAQASRV
                     AAALAAARFEQRPEIPIVFTAHSIPTAVASRSPYVEQLTTSARLVAARLGHPRWSVAY
                     QSRSGDPGTPWLGPDVNEAIRDLGRQGAAGVVIVPIGFVCDHIEVLYDLDTEARHTAE
                     EAGVAFCRSGTVADHPAFIRMLGDLVCAGAVT"
     gene            complement(3200..>4155)
                     /gene="hemE"
                     /locus_tag="VEW91_06605"
     CDS             complement(3200..>4155)
                     /gene="hemE"
                     /locus_tag="VEW91_06605"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HYM91287.1"
                     /translation="GGRYLPAYRAIRERLSLLELCKTPQAAAEVTLLPVDRLNVDAAI
                     VFADILLLAEPLGVGLEYAKGEGPVIQRPLRTPADVRQLPEVDPLDGVPFVFEAVRLA
                     RQALDGRVPLIGFAGAPFTLASYLIEGGPSQGFLRTKHLMHREPRTWQALMEKLSRLT
                     VRYLNGQIAAGAQAVQLFDTWVGCLSPDDYRRHVLPSTRQVISGIAGGVPVIHFGTGT
                     AGLLELMREAGGDVIGLDWRVDLGQAWRRLGDGVAVQGNLDPAVLLADLDEIRLGARA
                     VLASAGGRPGHIFNLGHGVLPETPWQHVAGLVEMVHEESRR"
BASE COUNT          637 a         1511 c         1442 g          565 t
ORIGIN      
        1 ggaacgcgcg gatcgggttt tccctgatcg ttctgttcac cctgatcgcg gtcttcgccc
       61 ccaggatcgc gcctgaaggg ttcgacgacg agaacctccc cgggcggctc catccgccga
      121 cggcccagca ctggattggg accgacgagt tggggcggga tgtgttcagc cgggtgatct
      181 acgggtctcg gatctcgatg atggtggggt ttgtcgcggt ggcgggagcg ctgacgacgg
      241 gatcgctgct gggcatcacc gccgggtacg tcggggggcg cgttgacgga ctgatcatgc
      301 ggatcatgga cgtcatgctg gcgttcccgt ccataatcct cgccatcggc atcgtcgcgg
      361 tgcgcgggcc cgggctcaac aacaccatcc tcgcagtgag catcgtggac atccccgtgt
      421 acgcgcgcgt cgcgcgggcg tcgaccctct cgatcaagga gcacgagtat gtgaccgcag
      481 cgcgcgcgat gggagcgagg gcgtggcgga tactgatgag ggcgatcctg ccgaacgccg
      541 cctccccgct cgtggtccag gcgaccctgg gggtggccgg cgcgatcctg aacgccgccg
      601 cgctgggctt cctggggctg ggggcccaac ctccgacgcc cgaatggggc gccatgctga
      661 gcgacagcta caaatacctg ctcacggcgt tttgggccgc cctggctcca ggcgttgcga
      721 tcgctgccat ggtgttgagc ttcaatctgg ccggcgacgg cctccacgac gccctcgaac
      781 cccgcctgtc ggagtcctag ctggcctggc cttgggcctg cgccagcacg cgctccgccg
      841 cccgttcccc cgagtggatg cagtccggga tcccgacccc gccgagtgcg ttgccagcga
      901 cggcgaggcc gggatggcgg gccacgcccc cctcgatcgc cgcgacccgc tcggcatgcc
      961 ccacctggta ctggggcatc gccgcgacat gccggtgcac ccgcacgagg tgcggagagc
     1021 cggagatccc gagcaggggc tcaagctcct gctgggccgc ggcaccgagc gccgcgtcgt
     1081 cgcgatccag cagttcccgg gcacgcgccc cgccgaggaa cacccggagc agcacgaccc
     1141 cgtctggagc ccggccgggg aacttgaggc tcgagaaggt gcatccgagg atctgccggt
     1201 cctccgcgtg cggcaccacg aatccgaacc cgtcgagcgc gtggcggatc tcctctcgtc
     1261 ggtatgccag cgtgacgatg gccgacgacg cgtaggaaat cgctgcgagc gcacggccga
     1321 ggtccggatc gagccccgcg accagccggg ccgcgcgcgg cgccgggatg gcgaggatca
     1381 cgccgtcggc cagcagcgcg gtgccgtccg ccaggttcac ccgccaggga cgggggccgc
     1441 gtgaccgcgc cagcgaggtg acccgccggc gcagcttcac cgacgcaggc gggagccgcc
     1501 gggccgcagc cgagaccaac tcccccatcc cgccagccag cgtcacgaag aggctccagc
     1561 gcggaccgct ttcctccacc gcggggcgcc ggtcttgcgc tcgacgaagc gcgcggatga
     1621 tgctcctatg ctgtcgttcc atctcgacga accgaggcat cgccgcagca aggctgacat
     1681 cgaagggatc cgcgccgtag atcccgctca cgaggggctg gaccacccgg tcgagcactt
     1741 cccgtccgaa ccgccgggtc acgaacgccc ccacgctttc gtccccgccc gcctgccgtt
     1801 tgggcaggac gaggtccatc gccagccgca gcttgccccg ccaggagagg aggggggacc
     1861 gcaagatggg tccgaggcgg ctcggcgcga ggaccaggaa tccatcgggg agcggcacaa
     1921 gccgaccgcg gcagacgatg aaggtccggc gggagccctc ccgcgtgggg accagtccgc
     1981 cggtcagccc caggcgatcg cagagggaca gtccccacgg cttctctgtc agaaacgagt
     2041 ccggtccctc ctcgatcagg tagccgtcgg cgcgctcggt cgcaacgacc ccgccaaggc
     2101 ggtcacgcgc ctccaccagg acgagctcca ccggcggtcg acgggcccga cccagctcga
     2161 cgagccggtg cgccgccgcg agccccgcga tcccgcctcc cacgacgacg acccgcagaa
     2221 gctcggcgcg gcttgggagc ggctgcggct tcgcgaggac gttctcggcg atcacgtcac
     2281 cgcgcccgcg cagacaaggt cgcccagcat ccggatgaat gccggatggt ccgccacggt
     2341 gcccgatcgg cagaaggcca ccccggcctc ctccgcggtg tggcgggcct ccgtgtcgag
     2401 atcgtacagc acctcgatgt ggtcgcacac aaacccgatg gggacgatca cgacgcccgc
     2461 cgccccctga cgcccgaggt cccggatcgc ctcattgacg tccgggccca gccagggggt
     2521 cccggggtcc ccgctccggc tctggtacgc caccgaccat cgggggtggc cgaggcgggc
     2581 ggcgacgagg cgggcggagg tcgtcagttg ctcaacgtag ggcgagcggg acgccaccgc
     2641 agtcgggatg ctgtgcgcag taaagacgat cgggatctcg ggccgctgct cgaaccgtgc
     2701 agcggccagc gcggccgcga cccggctggc ctgcgcctcg atgaaccccg ggtgatcgaa
     2761 ccacgcccga aggtagctca cctcgggcgc cccctcgccc acttgggcgc gcgcctctgc
     2821 gacgttctcg cggtactgat cccaactcga gtaactgtgg tggggcgcca tcacgatccc
     2881 cacggcacgc cgcacaccct ccgcccgcat gcacgccagg gtctcatgga ggtagggcgt
     2941 ccagttgcgc atccccacgt acacggggat cttcgaaccg cgcgcggcca ggtcggcttc
     3001 cagcgcccgg gcctggcggt gcgtgagctc ggtcaagggg gaatggccgc cgaaccgcgc
     3061 ataatgatgg accacctcgt cgatccgctc aggcgggacc cgccgtccgc ggaccacgtt
     3121 ggccaggaac ggccggacgt cttccatccg gtccggcccc ccaaacgcga tgagcagaac
     3181 ggcgtcgtag gtgggcatct cagcgccggc tctcctcgtg caccatctcg accagcccgg
     3241 cgacgtgctg ccacggggtc tcggggagca cgccgtgccc gaggttgaag atgtgcccgg
     3301 ggcgaccgcc cgcgctcgcg agcacggccc tggcgcccag ccggatctcg tcgaggtccg
     3361 ccagcaagac ggcggggtcc aggttgccct gaacggccac gccgtccccg aggcggcgcc
     3421 acgcctggcc gaggtccacg cgccagtcca ggccgatcac gtcgcctccg gcttcccgca
     3481 tgagctcgag caggccggcc gtcccggtcc cgaagtggat aaccggcacc ccgcccgcga
     3541 ttccggagat gacctgacgg gtggacggca gaacgtgccg gcggtagtcg tcgggcgata
     3601 ggcatcccac ccaggtgtcg aagagttgca ccgcctgtgc gccggcggcg atctgcccgt
     3661 tgagatagcg gaccgtgagg cgcgagagct tctccatcaa ggcctgccag gtgcgcggct
     3721 cgcgatgcat caggtgcttg gtccgcagga agccctgcga tggaccgcct tcgatcagat
     3781 aggacgcgag ggtgaacgga gccccggcaa acccgatgag cggcacccgc ccgtcgagcg
     3841 cctgccgggc cagccggacc gcctcgaaca cgaacggcac cccgtcgagg gggtccacct
     3901 cgggcagctg ccggacgtcc gcgggggtcc gcagggggcg ctggatgacg ggcccctccc
     3961 ccttcgcgta ttccagcccg accccgagtg gctcggcaag cagaaggatg tcggcaaaga
     4021 cgatcgcagc atccacgttc aggcggtcca ccggcagcag cgtcacctcc gccgcggcct
     4081 ggggcgtctt gcagagctcc aggaggctga gccgctcgcg gatcgcccgg tacgctggaa
     4141 ggtaccggcc cccct
//
LOCUS       DASQGH010000338         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32706, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000338 DASQGH010000000
VERSION     DASQGH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32706"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>1932
                     /gene="glyS"
                     /locus_tag="VEW91_06610"
     CDS             151..>1932
                     /gene="glyS"
                     /locus_tag="VEW91_06610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HYM91288.1"
                     /translation="MPQRLVFELGTEELPPGAAWEGLRQIREAAAPALRAARIPCGPV
                     SACSTPRRIALIVERVAPVQEDLVREVRGPAARIAFAPDGSPTKAAEGFARAQGVPPS
                     ALERRRTAQGEYVFARLRSPGGPTLKALADLLPALASGLAFPKAMRWGPDGIRFARPI
                     RWVLAVLGGHVVPFEFAGVRSGRVTYGHRTLSPAAITVADARGFEAKLRRHRVLLDPA
                     ARRRRITLAVARVAESVGGRAILDPDLLEETVQLVEWPEALAGTFASEFLTLPREVLI
                     TVMQHHQKYFAVEDAAGRLLPAFVAIRNGGTRGLRTVREGNEWVLRARLADARFFFEE
                     DRTRPLEARIGELGGLIVHEKLGTMAQKRERLIGLAERLGRLLALDNEQSAHLRRAAD
                     LCKADLVTQIVREFPELQGVAGGIYAQLDGEPAPVAQAIREQYLPRGSVLPRTEAGAY
                     LALLDKLDNLLGALAVGLAATGSEDPYGLRRAAQGIVAIVLDRKIRLDMRQIARDAPS
                     VNLGQLPPSALAQYSPFSQPVGAKDQYQLQHEAAVDAAMDLLRQRLRSVLIDSGISYD
                     TVDAVLAAGYDDLLDAAVRARALWAFRQ"
BASE COUNT          245 a          679 c          709 g          299 t
ORIGIN      
        1 tcgcggagcg ggcagcgctg atggggcggt gccggaccct cgcccgccgg tgtgccgacc
       61 tgtaccttgc ccgccgggag gagatgggct ggccgctgct ccgcgaccgc gtccatgccg
      121 cgtaggcgta cagcactgac ccggggggcg gtgccgcagc gcctcgtgtt cgaactcggc
      181 accgaggagt tgcccccggg ggcggcgtgg gagggcctgc ggcagattcg cgaggcggcc
      241 gccccggcgc tccgggcggc gcgaattccc tgcggtccgg tctccgcgtg ctcgacgccc
      301 cgccggatcg cgctcatcgt cgagcgggtg gcccccgtgc aagaagacct cgtccgggag
      361 gtccgcggcc ctgccgcccg gattgcgttc gcccccgacg ggagcccgac taaggccgcg
      421 gaggggtttg cacgcgcgca gggcgtgccc ccctccgccc tggagcgccg ccggaccgcc
      481 cagggggagt acgtcttcgc gcgcctccgg tcgcccggcg gccccacgct caaggccctc
      541 gccgatctgc ttcccgcgct cgcgtccggg cttgcgtttc ccaaagcgat gcgctggggc
      601 cccgacggga tccggttcgc ccgcccgatc cgctgggtgc tggcggtcct cggaggccac
      661 gtcgtgccct tcgagttcgc cggggtgcgg tcgggccgcg tgacctatgg ccaccggacc
      721 ttgagcccgg ccgcgatcac cgtcgcggac gcccggggct tcgaggcgaa gctcaggcgg
      781 caccgtgtgc tcctcgatcc ggcggcgcgg cgccgccgga tcaccctggc cgtcgccagg
      841 gtggccgaga gtgtgggggg ccgcgcgatt ctcgaccccg acctcctcga ggagacggtc
      901 cagctggtgg agtggccgga ggcgctcgcc ggcacgttcg cttccgagtt cttgacgctg
      961 ccgcgcgaag tgctcatcac ggtgatgcag caccaccaga agtactttgc cgtcgaagat
     1021 gcggccggac ggctgctgcc ggccttcgtg gccatccgca acggcggcac ccgcggcctg
     1081 cggaccgtgc gggaagggaa cgaatgggtg ctccgggccc ggctggccga cgcgcggttc
     1141 ttcttcgaag aggaccgcac gcgtccgctc gaggcgagga ttggcgagct cggcggcctc
     1201 atcgtccatg agaagctggg gacgatggcg cagaagaggg agaggctgat cgggcttgcc
     1261 gaacgtctcg ggaggttgct cgccctggat aatgagcagt ccgcccacct gcggcgggct
     1321 gcggacctct gcaaggccga cctcgtgacc cagatcgtgc gcgagttccc cgagctgcag
     1381 ggggtcgccg gcggcatcta cgcgcaactc gacggtgaac ccgccccggt cgcgcaggcc
     1441 atccgcgagc agtacctgcc gcgcggatcc gtgcttccca ggaccgaggc cggtgcgtat
     1501 ctggccctgc tggacaagct cgacaacctc ctgggcgcgc tggccgtggg tctggccgcg
     1561 accgggtcag aggatcccta cgggcttcgg cgggcggccc agggcatcgt ggcgatcgtg
     1621 ctggatcgca agatacggct ggacatgcgg cagatcgctc gcgacgctcc atctgtgaat
     1681 cttggtcagc tccctccgtc tgccttggca cagtactcgc cgttttcgca gcctgtgggt
     1741 gcgaaggacc agtaccagct ccagcacgag gccgccgtgg acgccgcgat ggacctcctc
     1801 cgccagcgcc ttcggtcggt gctcatcgat tcaggaatca gttacgacac ggtggacgcc
     1861 gtgctggccg caggctatga cgacctcctg gatgcggcgg tccgggcgcg ggcgctgtgg
     1921 gcgttccggc ag
//
LOCUS       DASQGH010000339         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46840, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000339 DASQGH010000000
VERSION     DASQGH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46840"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VEW91_06615"
     CDS             complement(<1..747)
                     /locus_tag="VEW91_06615"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91289.1"
                     /translation="MFISILSCNYGGGHRRVGETIAAEWESRTGGRAEVVDYFAEFVH
                     PVFDAVTKFSYIQSVRRAPGMYGMFYRATGEIKPDSLVQRAINRMGLERLDRYLEAKR
                     PDVVCCVHCTPAGTMSDLKILGRTTIPCLTVITDYVTHSQWIHPSVDRYCVPAAAVRD
                     GLQSRGVPGDRISVTGLPIAGKFLRPYDREPLMRRFGLVPHRPVVLVMAGAYAMLGGV
                     GDVVRVLARFPRPLQALVVCGHGQRLVQQVR"
     gene            complement(753..1706)
                     /locus_tag="VEW91_06620"
     CDS             complement(753..1706)
                     /locus_tag="VEW91_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HYM91290.1"
                     /translation="MSATLIVANLRSGAGRARDALPLVTNAFRRAGREYELIVTPTAE
                     ATAPLLAEFLGAERPGVDQVVVIGGDGTLNGAVNGMLASGSTLPLGIIPCGTGNEVVR
                     SLGIPKDPEKAVQIILRGQVCPVDVGKVNNRYFLHIFGLGYDVQVIKMVNMLRTRYRF
                     ARNRSVYYLASLLLLSSGFDPFPVRVQVGERVFSGRAIMIVAANGRMYGERLLALPAP
                     SLSDGLLDLYLLQELRTRSFRKTARLLRRLPVPELSVQRCSAVRIQLDQRREAQVDGS
                     LLGPAQTFDLSLLPRRISVMHPAPPALVGVPHGAEMGAERR"
     gene            1967..>3213
                     /locus_tag="VEW91_06625"
     CDS             1967..>3213
                     /locus_tag="VEW91_06625"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYM91291.1"
                     /translation="MAYAGGASEFGVRPVVLGRRGMVASANPLATQAGIQMLAAGGSA
                     VDAAIAAAVAVTVAEPYFSGLGGIGIAVVTLPTGETRTLNFMGHSPQSARPEIFTTQT
                     QDLGAMAPMVPGNVAGWARLHGEFGRLPLAQVLEPAIDLADRGIPVTVFDHERNVQAV
                     DRLTPHPDAARTYLHDGRPYNAGEVLRQPGLAETLRTIAEEGWMTFYDGRIGRTIGKY
                     LAEHGGLITADDLRGYPGTLKWEPPIVAAYRGYELRTTPPPSSAVQMLQTLRILEAFD
                     LHKMEHLGAEYLALVAEAIRLARMDSAANVADPLFFDVPIAWMLSDERVRELRAEVTR
                     RVDTSRARGRGRAGARGRTADRVRRARPAPERSTTHLAAADGSGLAVNITQTLGSSYG
                     AGGVIARTGIAMNNGHHWCTLVPG"
BASE COUNT          500 a         1121 c         1143 g          449 t
ORIGIN      
        1 gcgcacctgc tggaccagcc gctggccgtg cccgcagacg accagcgcct gtaacggccg
       61 cgggaaccgg gcgagcacgc ggacgacatc gccgacgccg ccgagcatgg cgtacgcccc
      121 ggccatcacc aggaccaccg ggcggtgggg gacgaggcca aaccgccgca tgagcggctc
      181 gcgatcatac gggcggagga atttgcccgc gatcggcagt ccggtgacgg agatccggtc
      241 cccagggacg ccgcggctct gcaggccgtc ccgcacggca gccgccggca cgcagtagcg
      301 gtccacgctc gggtggatcc actggctgtg cgtcacgtag tcagtgatca ccgtgagaca
      361 ggggatggtg gtgcgcccga ggatcttcag gtcggacatc gtcccggccg gcgtgcagtg
      421 gacgcagcag acgacgtccg ggcgcttcgc ttcgaggtat cggtccaggc gctccagccc
      481 catgcggttg atcgcgcgct ggacgagcga gtcgggtttg atctcgccgg tcgcccggta
      541 gaacatcccg tacatccccg gcgcccggcg gacgctctgg atatacgaga acttggtcac
      601 cgcgtcgaac accgggtgca cgaactccgc gaagtagtcg acgacctccg cccgtccccc
      661 ggtccgagac tcccattcgg cggcgatcgt ctcaccgacc cgacgatgtc cgccgccgta
      721 gttacacgag aggatcgaga tgaacattcc gcttaccggc gctccgcgcc catctccgcc
      781 ccgtggggta ccccaacgag cgccggcggc gcggggtgca tcacggagat cctgcgcggc
      841 agcagcgaga ggtcgaaggt ctgggccggg ccgagcaggc tgccgtccac ctgggcctcc
      901 cgacgttggt cgagctggat ccgcaccgcg gagcatcgct ggacgctcag ctcggggacc
      961 ggcaggcgcc gcagcaggcg ggcggtcttg cggaacgaac gggtgcggag ctcctgcagc
     1021 aggtagagat ccagcaggcc gtccgagagg ctgggggccg gcagggcgag caggcgctcc
     1081 ccatacatcc ggccgttggc cgcgacgatc atgatggccc gcccgctgaa cacccgttcc
     1141 ccgacctgca ccctgacggg gaagggatcg aaccccgacg agagcaggag gaggctcgcc
     1201 agatagtaga ccgaccggtt gcgggcgaac cgataccggg tgcggagcat gttgaccatc
     1261 ttgatcacct ggacgtcgta ccccagcccg aagatgtgga ggaagtaccg gttgttgacc
     1321 ttcccgacat cgacggggca cacctggccg cggaggatga tctggacggc cttctccgga
     1381 tccttgggaa ttcccagcga tcggacgacc tcgttgccgg tcccgcaggg gatgatgccg
     1441 agcggcaggg ttgagccgct tgccagcatg ccattcacgg ccccgttgag cgtgccgtcc
     1501 ccgccgatca ccacgacctg gtcgaccccc ggccgctcgg cgccgaggaa ctcggcgagc
     1561 aggggggccg tggcctccgc cgtcggcgtc acgatcaact cgtactcccg gccggcgcgt
     1621 ctgaaggcgt tggtgacgag gggaagggca tcgcgtgcgc gcccggcgcc ggagcgaagg
     1681 ttcgccacga tcagcgtcgc ggacatccca tcactcctcc gcagtccgcg ccctcgagaa
     1741 aagggcgcag tacgctgctg tggtgaggca tctcgaccgt cttattatcg cctgaatgcg
     1801 gtgacgtgtc aattttccca gcggccatct gctctcgctg ggacgctcgt ccaggcgctc
     1861 ccgcatgcgg tcagaggaat caggcgcggg gcgtagaagc ccgcagcgca ccgcatcgtg
     1921 cccagcgcgg cgaccgttcg cccgcatgtt caccaggagg tgccccgtgg cgtacgcagg
     1981 aggcgcgtct gagtttggcg tccggccggt cgtcttgggc cgccgcggca tggtggcatc
     2041 ggccaacccg ctcgccacgc aggcggggat ccagatgctc gccgcggggg ggagcgccgt
     2101 cgacgcggcc atcgcggcgg ccgtggccgt cacggtggcc gagccatact tctccggtct
     2161 gggcggcatc gggatcgccg tggtcaccct cccgaccggc gagacgcgga cgctcaactt
     2221 catggggcac agcccgcaat ccgcccggcc ggagatcttc acgacccaga cccaggacct
     2281 cggggcgatg gcgccgatgg tgcctgggaa cgtggcgggg tgggcccgcc tgcacggaga
     2341 gttcggtcgg ctgccgctcg cgcaggtgct cgagccggcg atcgacctcg cggatcgcgg
     2401 gatccccgtc acggtgttcg accacgagcg gaacgtccag gcggtggacc ggctgacccc
     2461 ccaccccgac gccgcgcgca cgtaccttca tgacggccgg ccctacaacg ccggggaggt
     2521 gctccggcag cccggccttg ccgagacgct ccggacgatc gccgaagagg ggtggatgac
     2581 attctatgac gggcggatcg gccggacgat cggcaagtac ctggctgagc acggcgggtt
     2641 gatcaccgcg gacgacctgc ggggctaccc cggcaccctc aagtgggagc cgccgatcgt
     2701 cgccgcgtac cgcgggtacg agcttcgcac cacgccgccc ccgagcagcg cggtgcagat
     2761 gctgcagacg ctgcggattc tcgaggcgtt cgacctgcac aagatggagc acctcggcgc
     2821 ggagtacctg gccctggtgg ccgaagcgat ccggctggcg cgaatggact cggccgccaa
     2881 cgtggcggat cccctgttct tcgacgtgcc gatcgcgtgg atgctgagcg acgagcgggt
     2941 gcgcgagctt cgggccgagg tcacccgtcg cgtggacacc tcccgggcgc gcgggcgcgg
     3001 tcgcgccggg gcccgcggcc gcaccgccga ccgggtccgc cgggcccgtc cggcccccga
     3061 gcgctcgacc acccacctgg ccgcggcgga cgggagcggg ctcgcggtca acatcacgca
     3121 gaccctcggc tcgagctatg gggcgggggg ggtcatcgcc cgcaccggca tcgcgatgaa
     3181 caacgggcac cactggtgca cgctggtccc cgg
//
LOCUS       DASQGH010000340         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38362, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000340 DASQGH010000000
VERSION     DASQGH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38362"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEW91_06630"
     CDS             complement(<1..250)
                     /locus_tag="VEW91_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HYM91292.1"
                     /translation="MTRGEAWAVLTEHTGNPNLLKHMLAVEAVMRGYARRFGEDEETW
                     GVVGLLHDLDYEKHPSREAGHPFVGVEVLRARGLDDRLC"
     gene            452..1990
                     /locus_tag="VEW91_06635"
     CDS             452..1990
                     /locus_tag="VEW91_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYM91293.1"
                     /translation="MDVHLDRAADTPLYRQLQTALTGRIRSGEIGAGSRLPSVRSLAR
                     SLAVSPITVVQAYNALAADGLIHASVGRGTFAGAANPEAVLRHDLPHPDAQAPGAPED
                     GEWHATLPVHLRAPRISAMQALLRPGRRPGMISLAAGTPDPNLFPIRALGRLWSRVMA
                     MEDPRFLQYGTPQGDLHMRTWIADRCATLGIAAKPEEVLITSGSQQAIDLVARTFVGP
                     GDYVLVESPTFITALDIFENRGAKLVGVPMDGEGARVDIAASLAERFRPRLIFTMPTA
                     HNPTGITMAEERRRHLAALARRYNLLILEDDTCSEFAYDGEAPPAIKAFDTSGHVIFI
                     QSFSKTVVPGLRVGCVVAQGPLLGSLVESKSVADRFTSPLIQRTLWRYLSSGQYPRDL
                     AAARDTYRRRRDAILRVLAETMPPGVTWTHPSAGFNLWIHLPDRTPPSDVFEEALKEG
                     VACAPGDLFLPHLPPPSGLRISFADSPEDVAGEGVKRLARAITRILASSRAEEQEADF
                     VTTV"
     gene            complement(1999..2850)
                     /locus_tag="VEW91_06640"
     CDS             complement(1999..2850)
                     /locus_tag="VEW91_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYM91294.1"
                     /translation="MGRRGLGRGLGALIPGADVERLAGLVQDLDIASISPNPLQPRQG
                     RPGPEFEELVASIRRHGVLQPVVARPADGGYQIVAGERRFRAAQAAGLTSVPAIVRDI
                     SDREALEVALIENLRREDLTPMDRARAYHRLATEFGLTQEEMAEAIGGSRPSIANTVR
                     LLDLPEEVQLALDQGRLTEGHGRALLTVQDRQRLLEIWRQVEKRGLSVRETEGLVKAT
                     ARNVSRETISPRTAGQDPILADLAARLQDRYATSVSIASKGKRGMIQINYYSADDLER
                     LIDILLR"
     gene            complement(2843..>3290)
                     /locus_tag="VEW91_06645"
     CDS             complement(2843..>3290)
                     /locus_tag="VEW91_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYM91295.1"
                     /translation="LAASDGCLIPVQCEYYALEGLSALLTSIELVRRHLNPPLRITGV
                     LLTMVDHRINLSAQVAAEVRRHFGPQVFETVIPRSVRLAEAPSHGQPIFSYASDSKGA
                     EAYLSLAHEMMGRIGLEPGWIAPGLVGVSGPVAATSSDNGGDDTHG"
BASE COUNT          565 a         1146 c         1017 g          562 t
ORIGIN      
        1 ggcagagtcg gtcatcgagc ccgcgcgccc gcagcacctc cacccccacg aagggatggc
       61 cagcttcccg cgaaggatgc ttctcgtagt ccagatcatg cagcagcccc acaacccccc
      121 aggtctcctc atcctcgcca aaccggcggg cgtagccgcg catcaccgcc tccacggcca
      181 gcatgtgttt gaggaggttc gggttgccgg tgtgctcggt cagaactgcc cacgcttctc
      241 cccgggtcat ctcgacgggt ccacctctct gatcctaggc gtaatcgctg ctattgagta
      301 gtgtatctaa taacactact tgactatttc aagatatcag cgtagtgtta ttgtacaatt
      361 cgctgcgaat ctgtcaatat acaccatata ctcatacgta ctgataggtt cacaccatca
      421 cacattaagg gcagaggact cggagcggca aatggatgtg caccttgata gggcggccga
      481 cacccctctc tacagacagc ttcagacagc gctgaccggc cggatccgat ccggggagat
      541 cggtgcgggc tcccggctcc cctcggtaag aagcctcgcc agaagcctcg cggtgagtcc
      601 cattaccgtc gtccaggcct acaacgccct ggccgcagat gggctgatcc acgcctcggt
      661 cggacgggga acgtttgcgg gcgccgccaa tcccgaggcg gtgcttcgcc acgatctccc
      721 acatcccgac gcccaggccc cgggagcccc ggaggatggg gaatggcacg ccacgctgcc
      781 ggtccacctt cgggccccca ggatcagcgc gatgcaggcg ctcctgaggc ccggacgccg
      841 gcccggcatg atctccctcg ccgcgggaac gccagaccca aacctctttc ccatccgggc
      901 actcggcagg ctgtggagcc gcgtgatggc gatggaagat ccccgattcc tccagtacgg
      961 cacgccccag ggagacctcc acatgcggac ctggatcgcc gaccgctgcg ccacgctcgg
     1021 gattgcggcc aagcccgagg aggtcctcat tacctccggc tcccagcagg ccatcgacct
     1081 ggtggcgcgc acctttgtcg ggccgggcga ctatgtgctg gtcgaaagcc cgacattcat
     1141 cacggctctg gacatcttcg agaatcgcgg ggccaagttg gtcggcgtgc ccatggacgg
     1201 tgaaggcgcg cgggtggata tcgccgcctc gctcgcggag cggttcaggc cgcgcctcat
     1261 cttcacgatg cctaccgccc ataacccgac cgggatcacc atggccgaag agcgccgccg
     1321 ccatctggcc gccttggccc gccggtacaa cctgctcatc ctggaagacg atacgtgcag
     1381 cgagtttgcc tacgatgggg aagccccccc tgcgatcaag gcgttcgaca caagcgggca
     1441 tgtgatcttc atccagagct tctccaagac ggtcgtgccc gggctccgcg tcgggtgcgt
     1501 cgtggcccaa ggccccctcc tggggagcct ggtcgaatcc aagtccgtgg cagaccggtt
     1561 tacgtctccg ctcatccagc gcacgctctg gaggtacctg tcctcgggcc aatatcctcg
     1621 cgatcttgcc gccgccaggg atacctaccg ccggaggagg gacgctatcc tgagggtgct
     1681 ggcggagacc atgccaccgg gggtcacctg gacccacccg tcggcgggat tcaacctatg
     1741 gatccacctg cccgaccgca cccccccatc cgacgtgttc gaggaggcgc tcaaggaggg
     1801 ggtcgcatgt gcgccgggag accttttcct gccccacctc cccccaccct ccgggctcag
     1861 gatctcattt gccgatagcc cggaagacgt ggccggcgag ggggtcaaac ggctggctcg
     1921 ggcgatcacc cgcatcctgg cctcctcgag agccgaagaa caggaagcgg actttgtgac
     1981 gactgtttag acgggggcct accgcaacag gatgtcgatc agccgttcaa ggtcatccgc
     2041 cgagtagtag ttgatctgaa tcatgccccg ctttcccttg ctcgcgatgc tgacgctggt
     2101 ggcgtagcgg tcctggagcc gggcagccag gtcggccagg atcgggtctt gcccggcagt
     2161 cctcggggaa attgtttcac gtgaaacatt cctggccgta gccttgacca ggccctcggt
     2221 ctcccggacc gagagccccc tcttctccac ctgccgccag atctccagga ggcgttggcg
     2281 atcctggacg gtgagcaggg cccggccgtg gccctccgtg agcctgccct gatcgagggc
     2341 gagctgcacc tcctcgggaa ggtccaagag ccgcacggtg ttggcgatgg aaggccggct
     2401 gccgcctatg gcctcggcca tctcctcctg ggtcagcccg aactcggtcg ccagcctgtg
     2461 gtacgcccgg gcccgatcca tcggggtgag gtcctcgcgc ctcaggttct cgatcagcgc
     2521 gacctcgagc gcctcccgat ccgagatgtc cctgacgata gccggaaccg aggtcagccc
     2581 ggcggcctga gccgcccgaa accgccgctc tccggccaca atctggtacc ctccgtcggc
     2641 cggtcgtgcg acaaccggct ggaggacacc gtgcctccgg atcgaggcca cgagctcctc
     2701 gaactcgggc cccggcctgc cctgcctggg ctgcaatggg ttgggggaga tcgacgcgat
     2761 gtccaggtcc tgcaccagcc cggcgaggcg ctcaacgtcc gcccccggga tcagcgcgcc
     2821 gagcccgcgt ccaagtcctc gcctacccat gcgtgtcatc ccccccgttg tctgacgatg
     2881 tggcagcgac cgggcccgat acccccacga ggcctggcgc gatccacccg ggctccaacc
     2941 cgatgcgtcc catcatctcg tgggccaggc tgagataggc ctcggcgccc ttcgagtccg
     3001 aagcgtagga aaatatcggc tgaccgtgac tgggggcctc cgccaggcgt acgctcctcg
     3061 ggatcaccgt ctcgaaaacc tgggggccaa aatggcgcct gacctccgcg gcgacctgcg
     3121 cggaaaggtt gatcctgtgg tcgaccatgg tgaggaggac cccggtgatc cgtagggggg
     3181 ggttcaggtg tcgccggacg agctcgatcg aggtgaggag agccgaaagc ccctcgagcg
     3241 catagtactc gcactgcacg gggatcaggc acccgtcgga cgcagcgagc
//
LOCUS       DASQGH010000341         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35494, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000341 DASQGH010000000
VERSION     DASQGH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35494"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VEW91_06650"
     CDS             <1..395
                     /locus_tag="VEW91_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYM91296.1"
                     /translation="LEWARTLARAFGAKIVLLHVVDLAVEWTPVGGLGSIPAPVPAEF
                     VERFTNDAQVALDAIAQGTPPVTQRLMRKGHPREVILDVAREVGADVIVMGTHGRRGF
                     SHLFIGSVAEHVVRHAPVPVWTVRAPEP"
     gene            502..732
                     /locus_tag="VEW91_06655"
     CDS             502..732
                     /locus_tag="VEW91_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91297.1"
                     /translation="MHRYEREFNTLVVLEQRLLNARSEVGVAEHLVGHGQLDEEIRAI
                     AEALDDSIALAQRAKHIMLNEWRAAEASQAAA"
     gene            1078..2262
                     /locus_tag="VEW91_06660"
     CDS             1078..2262
                     /locus_tag="VEW91_06660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYM91298.1"
                     /translation="MSDRRTGFPEIQGFTRQGFEAVREAFVENFSRRNELGAACCIYH
                     RGEKVVDLWGGIRNKATSDPWEEDTMVLVFSTTKGLAAMTMAVAHSRGWLDYEERVCT
                     YWPEFAQQGKDKITVRQLLSHQAGQFAFDEPVDKGVVADLDRLAVVLARQKPAWEPGT
                     RQAYHAISLGFYEGELLRRVDPKHRSLGQFFQDEIASPLGLDFYIRLPEAIPKARLAT
                     IEKGNPLKMIVRLPLPLMLASMNPRSNIYRALVVNPGGWLPLDEERVYARNLEVPSGG
                     GIGTARAIAHAYGVFATGGRELQLRPETLQALAAPAVPPLRGFYDECLKGEIQFSLGF
                     MKPSAPWPVGHPGAFGAPGAGGSLGFADPQAGIGYAYVTNRMGVNTGDPRDLALRNAI
                     PH"
     gene            complement(2327..2893)
                     /locus_tag="VEW91_06665"
     CDS             complement(2327..2893)
                     /locus_tag="VEW91_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYM91299.1"
                     /translation="MRDDRVRWDQKHERGVETLEGPPVAYLVAWLPFLPRGRALDLAA
                     GSGRHAIYLAQHGYRVDALDISLVGLARLRQQARAQALPVRAAAVDLDECLLSSATYD
                     LIVNTYYLNRFILPQLPWALVPGGALLVETPLYDPRTDRPGEIARRVGPGELARVFSN
                     LDIAHYEEVPARPPQRNHGVCRMVAFRS"
     gene            complement(2905..>4443)
                     /locus_tag="VEW91_06670"
     CDS             complement(2905..>4443)
                     /locus_tag="VEW91_06670"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HYM91300.1"
                     /translation="LPEQGSIRRGRYVLVSAITPTPLGEGKTLTAIGLSMALGRLGRR
                     TAVTIRQSSLGPSFGAKGGGAGGGAARIEPFEECLLGLGGDAYAVESANNLLAAFIDD
                     ALLRRAAPLDPFSVTWRRVVDVDDRALRHVVVGLGGRLHGVPRETGFDITAASEVMSI
                     LALSRDLRDLRRRLGAIVVGFDPEGRPVAAEGLRCAGAMAVLLREALQPNLMQTSEGT
                     PALVHAGPFGNISHGSSSVIADLLVLPRVDYLVTEAGFGAEMGAEKFFHIKCAASGLV
                     PDAAVLVATVAGLKSHSGRPVPAERRQESVADVEAGSVNLTAQIRNLRLFGVPVVVAI
                     NRFPTDTEAELQVVQTQARAAGAAAVAEHRAFERGGEGCLALADAVEDACRLGTAFHP
                     LYSPEDSPRKKLEMLATRLYGAAAVSFSAEAERDLARYVSAGYGSLPLCVAKTHLSLS
                     HDPALKGAPSGYTFPVNGMRLAAGAGYLYALAGDIVTMPGLPSHPRAVGIDLDEAGEI
                     VGLT"
BASE COUNT          753 a         1484 c         1504 g          702 t
ORIGIN      
        1 ccctcgaatg ggcgcggacg ttggcgcgcg catttggcgc aaagattgtc ttgctccacg
       61 tcgtcgacct tgcggttgag tggacaccgg tggggggact cggatcgatc cccgcgccgg
      121 tccccgcaga gttcgtcgaa cgattcacca atgacgctca ggttgctctc gatgcgatag
      181 cgcagggcac gccgccggtg acgcagcggc tgatgcgcaa gggccatccc cgcgaggtca
      241 tcctcgacgt cgcacgagag gtcggcgccg acgtcatcgt gatggggacc catggccgtc
      301 gtggcttctc ccacctgttc atcggcagcg tcgccgagca cgtcgtcagg cacgccccgg
      361 tgccggtgtg gacggtgcgc gccccggagc cgtgaaggaa gccctcgcgc gatccgtcgg
      421 gcagagtagc ccgaatcggg cccgagtccg atgggcaggc cgcgcgccct gggcggatga
      481 taggcaggga ggtggacttc gatgcaccgt tacgagaggg agttcaacac cctggttgtc
      541 ctcgagcagc gcctcctgaa cgccaggagt gaggtggggg tcgcggagca cctcgtcggc
      601 cacgggcaac tggatgagga gatccgggcg atcgccgagg cgctggacga cagcatcgcc
      661 ctcgcccagc gggcgaagca catcatgctc aacgagtggc gggccgccga ggcaagccag
      721 gcggcagcgt aggccgcacc cgatccggcg ccggcaagcg gttccgcctc tactcgatcg
      781 gggcaatcct ggtcctcgtc gtgtttggcg cgttgacggg tttggacggt cccgggttgc
      841 ggcaaacctg cctacgccgc ggctcggtgt gaccgagcgc atcaacgtct tcggttacct
      901 gctatgggcc atcaccctct tgcgcgtccg agataccgcg gccccaagat agcttcggtg
      961 ggagaggtga ttccatggac gccttcgtgg tcgcgcggct gatacagcgc gagttcacga
     1021 tgtctagaag tgttctcgga acctggctgc tcacagggag atgaagggtc ccagacgatg
     1081 agcgatcggc gtacgggatt tccggaaatt caggggttta cccgccaggg ttttgaggcc
     1141 gtgcgagagg cgtttgtcga gaacttctcg cgacgaaacg agctcggggc cgcctgctgc
     1201 atctaccacc ggggcgagaa agtggtcgac ctgtggggcg gaattcggaa caaggcaaca
     1261 agcgatccct gggaagagga caccatggtt ctggtgttct cgacgaccaa gggcttggcc
     1321 gccatgacca tggcggtcgc ccattcgcgg ggttggctcg actacgaaga gcgcgtgtgc
     1381 acgtactggc cggagttcgc gcaacagggc aaggacaaga tcaccgtccg gcagctcttg
     1441 tctcatcagg cgggacagtt cgcgttcgat gagccggtcg ataaaggagt ggtcgccgac
     1501 ctcgatcggc tcgccgtggt cctggcacga cagaaaccgg cctgggagcc aggaacgcgg
     1561 caggcctatc acgccatcag cctcgggttt tacgaaggcg agctcctgcg ccgggtcgat
     1621 ccgaagcacc gcagcctggg acagttcttc caggacgaaa tcgcttcgcc gctggggctg
     1681 gatttctaca tccggcttcc cgaggccatc cccaaggcca ggctggcgac gatcgagaag
     1741 ggcaatccgc tcaagatgat cgtgaggctc ccgttgccgc tgatgctggc gtcgatgaat
     1801 ccgcgctcaa acatttaccg cgcccttgtg gtgaatccgg gaggctggct gcctcttgac
     1861 gaagaacgcg tatatgcccg caacctcgaa gtgccgtctg gagggggcat cggcaccgcg
     1921 cgggcgattg cgcacgccta cggcgtcttc gctaccggag gccgtgaact gcagttgcgt
     1981 ccggaaacgt tgcaagcgct ggcggcgccg gcggttcccc cgctgcgcgg cttttacgac
     2041 gaatgcctga agggcgagat tcagttttcg ctgggcttca tgaagcccag cgcgccatgg
     2101 cccgtcggtc atccaggcgc gtttggggcg cccggagcgg gcggttctct cggtttcgcg
     2161 gatcctcaag ccgggatcgg atatgcgtac gtcacgaatc gaatgggcgt gaacacaggg
     2221 gatcccaggg atctggcgct gagaaacgcg attccgcact aagatcaatt tcagctgaga
     2281 cggggaaaag gggaccaaat gaagcggaag gacatttata tctcgttcag ctgcggaacg
     2341 ccaccatccg gcacacgccg tggttcctct ggggaggtcg ggccggcact tcctcgtagt
     2401 gcgcgatatc caggttggag aagacccggg cgagctctcc tggccccacg cgccgcgcga
     2461 tctcgccggg acggtcggtc cggggatcgt acaggggggt ttccacgagc agcgcgcccc
     2521 cggggaccag cgcccagggt aattgcggca ggatgaagcg gttcagatag taggtgttga
     2581 cgatcaggtc gtaggtcgcc gaggacagca ggcactcatc gagatctacc gcggccgccc
     2641 gcacaggcag ggcctgggcc cgcgcctgct gcctcagccg ggcgagaccg acaagcgaga
     2701 tgtcgagcgc atccacccga tatccgtgct gcgccagata gatcgcgtgc cggccgctcc
     2761 ccgccgccag atcgagggcg cgccccctcg ggagaaaggg cagccaagcc accaggtagg
     2821 ccaccggcgg cccctcgagg gtctcgacgc ccctctcgtg cttctgatcc cagcgtaccc
     2881 ggtcgtcgcg catccaaccc tcgttcaggt aaggcccacg atctcgccgg cctcgtccag
     2941 atcgatcccc accgcccgtg gatggctcgg cagccccggc atcgtcacga tgtccccggc
     3001 gagagcatag agatacccgg ctcccgcagc cagcctcatt ccgttgacag gaaacgtgta
     3061 cccgctcggg gcgcctttga gggccggatc gtgagagagc gagagatgcg tcttggccac
     3121 gcagaggggc agggacccgt agccggccga gacgtagcgc gccaggtctc gttccgcttc
     3181 ggcggagaac gacaccgctg cggcgccgta cagccgggtg gccagcatct ccagcttttt
     3241 cctgggcgag tcctccgggc tgtagagggg atggaacgcg gtccccagcc ggcaggcgtc
     3301 ctcgaccgca tccgccagcg cgaggcagcc ctccccgccg cgctcaaacg cccggtgctc
     3361 ggccacggcc gccgccccgg ccgcgcgggc ctgcgtctgc accacctgaa gctccgcctc
     3421 ggtgtccgtg gggaagcggt tgatggcgac caccaccggg actccgaaga ggcgtaggtt
     3481 ccggatctgc gcggtgaggt tgaccgaccc cgcctccaca tccgccacgc tctcctgccg
     3541 gcgctcggcg gggacgggtc ggccagagtg gctcttgagg cccgcgaccg tggccaccag
     3601 cacggcggcg tccgggacca ggccggacgc cgcgcacttg atatggaaga acttctcagc
     3661 gcccatctcg gcgccgaagc ccgcttcggt cacgaggtag tcgacccggg ggagcacgag
     3721 caggtccgcg atcaccgagg agctcccgtg ggagatgttc ccgaaggggc cggcatggac
     3781 caaggcgggc gtcccttcgg acgtctgcat caggttcggc tggagcgcct cccgcaagag
     3841 caccgccatc gccccggcgc agcgcagtcc ctccgcggcg accgggcgcc cctccgggtc
     3901 aaagcccaca acgatcgccc ccagccgccg ccggagatcc cgcagatccc ggctgagcgc
     3961 gaggatcgac atcacctcgc tggccgcggt gatgtcgaag cccgtctccc gcggcacccc
     4021 atggagccgg ccccccaagc cgacgaccac gtggcgcagg gccctgtcgt cgacgtccac
     4081 gacccggcgc caggtcaccg agaacggatc gagcggggcg gcccgccgca gcagcgcgtc
     4141 gtcgataaac gcggccagca ggttgttggc gctctccacg gcgtacgcgt cacccccgag
     4201 gccgagcagg cattcctcga acggctcgat ccgtgccgcg ccgcctccgg cgccgccgcc
     4261 cttcgcgccg aaactcggac ccagagaaga ctggcggatc gtcaccgccg tccttctgcc
     4321 cagccgcccg agcgccatcg agagcccgat ggcggtcagc gtcttgccct cgcccagcgg
     4381 tgtcggcgtg atggctgaga cgagcacata cctgccgcgc cgaatgctcc cctgctcagg
     4441 cag
//
LOCUS       DASQGH010000342         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_29872, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000342 DASQGH010000000
VERSION     DASQGH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29872"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VEW91_06675"
     CDS             <1..1439
                     /locus_tag="VEW91_06675"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYM91301.1"
                     /translation="KTDLIVALDIVVITLVVSISGNIDSPFVYLYYLTIMEAAARLDV
                     RQAIAASLAMTGMLVIFWTRAGQGAVLETVGFRLGAVIAGGFFLALFLGMLVQKYRAS
                     RERTWWGAQLDQARAQRTAELEAFHDLTRQLRTGRNTDEISPILVDHAIELLQADHGM
                     LVVLSPDHETLTRLYSAGLSPEGLAETFPVAGSPAGHVIQTGAPLVADDLAGLAVIPG
                     FDPAPYRAFGPTVAVPVASEQEIIGTLELERARGSNGRPFTDADVRLLEGIAEIGGNA
                     LRRARLFHNLEQAYIEMVLALARTMDARDAYTAGHSERIAGLAETVARALGCGAEEIQ
                     DIRWGALLHDIGKIGVPDEILRKPGPLIASELAVMRQHPIVGEGILAPTERMRGVAKI
                     VRHHQERWDGSGYPDGIRGEAIPLGARILAVVDAFSAITDDRPYKKAQSHELALTELR
                     RGAGTRYDPRIVEIFCGLRRARLRKRSA"
     gene            1436..2533
                     /locus_tag="VEW91_06680"
     CDS             1436..2533
                     /locus_tag="VEW91_06680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYM91302.1"
                     /translation="MTEGSVRKRRRAQRPEESRVPSAPASGTGVPTESERGLTFLARL
                     AAEFTTVLSLSDLLEHVITVLREETGFDSCAVSLLAKQNTEDVLVVRAASGLREAMKG
                     TVFPRGKGLVWAVMDSGTPLLITDLRAEPRWFLPDSRIRAGIYAPLVVHRRPIGVVSA
                     YRGGVGGFTESDLNLLTVVARYLAGAVEVARLHEQLKELAATDSLTGLANRRAFLDRL
                     QSEIARSQRRGYEFCVVLLDLDRFKAVNDIYGHAVGDQVLIRVAEALSQALRQSDLAG
                     RFGGDEFILLLPESNRAQGAEILDRLRTAPIFIPDRDGRGVRTTFSWGIATWPRDGQE
                     AERLLQMADRRLYAMKRGQRPHDPQSAPAAD"
     gene            2637..>3446
                     /gene="ugpC"
                     /locus_tag="VEW91_06685"
     CDS             2637..>3446
                     /gene="ugpC"
                     /locus_tag="VEW91_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYM91303.1"
                     /translation="MAKVLLEQITKQFGNVLAVNNVTLDVPDKQFTVLVGPSGCGKTT
                     CLRLVAGLEEATAGNIYIGERLVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGLRL
                     RKNARAEIDRRVKEAAEMLGIQELLSRKPKQLSGGQRQRVALGRAIVREPQVFLMDEP
                     LSNLDAKLRVQTRAEIKKLHARLQTTTIYVTHDQVEAMTMGDRIVVMKDGLVQQVDSP
                     LSLYEKPANLFVAGFIGSPAMNFLEAKLAKQDGRIVVDGDVFRAEVPQEFVP"
BASE COUNT          572 a         1123 c         1210 g          541 t
ORIGIN      
        1 agaagaccga cctgatcgtt gccctggata tcgtggtgat cacccttgtc gtcagcatct
       61 caggaaacat cgacagcccg ttcgtctacc tgtactacct gacgatcatg gaagctgccg
      121 cccggctcga cgtgcgccag gcgatcgctg cgtcgctggc gatgaccggg atgctcgtca
      181 ttttctggac gcgggcaggg cagggtgcgg tgctggagac ggttggcttt cggctgggcg
      241 cggtgatcgc gggcggattc ttcctcgcgc tttttctcgg catgctcgtc cagaagtacc
      301 gggccagccg cgaacgcacc tggtggggcg cgcagttgga tcaggcccgc gcgcagcgga
      361 cggccgaact tgaggccttt cacgacctga ccaggcagct gcgcacgggg cgcaacaccg
      421 acgagatctc cccgatcctg gtcgatcatg ccattgagtt gctccaggcc gaccacggga
      481 tgctcgtggt gctctcccct gatcacgaga cgctcacccg gctctacagc gccgggctgt
      541 ccccggaggg tctcgcggag acgtttccgg ttgccgggag cccggcaggg cacgtgatcc
      601 agacgggagc cccgctggtg gcggacgacc tcgccggcct ggcggtcatc cccgggttcg
      661 atcccgcacc ctatcgggcg ttcggcccca ccgtcgccgt gcccgtggcg tccgagcagg
      721 agatcatcgg gacgctggag ctggaacgcg cccgggggag caacggccgc ccgttcacgg
      781 acgccgacgt ccgcctgctc gaaggcatcg cggaaatcgg agggaacgcg ctccgccggg
      841 cgcgtctctt ccacaacctc gagcaggcgt acattgagat ggtcctggcg ctggcccgca
      901 cgatggacgc ccgggacgcc tacaccgccg gccacagcga gcgcatcgcc ggcttggccg
      961 agaccgtggc cagggccctg gggtgcggag cggaggagat tcaggacatc cgatggggcg
     1021 cgctgcttca cgacatcggg aagatcggcg tccccgatga aatcctgcga aagcccggtc
     1081 ccctgatcgc atccgagctg gccgtgatgc gccagcaccc gattgtgggg gaggggatcc
     1141 ttgcccccac cgagcggatg cggggagtgg ccaagattgt gcgccaccat caggagcgct
     1201 gggatggatc cgggtatccc gatggcatcc gcggcgaggc gattccgttg ggagcccgca
     1261 tcctggccgt ggtcgacgcg ttcagcgcga tcaccgacga ccggccgtac aagaaggccc
     1321 agtcgcatga gctggcgttg acggaactcc gccgcggcgc ggggacgcgg tacgaccccc
     1381 ggatcgtcga gattttctgt ggcctgcgcc gggcccggct aaggaagcgg agcgcatgac
     1441 cgaaggatcg gtgcgcaagc gcaggcgggc ccagcggccg gaggaatcgc gcgtgccgtc
     1501 cgcgccggcg agcggcaccg gggtgccgac cgagtcggag agagggctga ccttcctggc
     1561 ccggctcgcg gcggagttta ccacggtcct gagcctgtcc gacctcctgg aacacgtcat
     1621 cacggtgctg cgcgaggaaa cagggttcga ttcctgcgcg gtctcgctgc tcgcaaagca
     1681 aaataccgag gacgtgctcg tggtccgcgc cgcatcaggg ctccgcgagg cgatgaaggg
     1741 gaccgtcttc ccccgcggga aggggctcgt gtgggccgtc atggattccg gtaccccgct
     1801 gctgatcacc gacctgagag ccgagccgcg atggtttctg ccagattccc gaatccgggc
     1861 cggcatctat gcgccgttgg tcgtccaccg gcgtccgatc ggcgtggtca gcgcctaccg
     1921 gggaggggtc gggggcttca cggagtccga cctcaacctg ctcaccgtcg tggcgcggta
     1981 cctcgcgggc gcggttgagg tggcacgcct gcacgagcag ctgaaggagc tcgcggcgac
     2041 cgattcgctg accgggctgg cgaaccgccg cgcctttctc gaccggctcc agtccgagat
     2101 cgcacgcagc cagcgcaggg ggtacgagtt ctgcgtcgtc ctcctcgacc tcgaccgctt
     2161 caaggccgtc aacgacatct acggccacgc ggtcggggac caggtgctca tccgggtcgc
     2221 agaagcgttg agccaggccc tgcgccagtc cgacctggcg gggcggttcg ggggggacga
     2281 gtttatcctc ctacttcccg agtcgaaccg cgcgcagggt gcggagatcc tcgatcgact
     2341 tcgcaccgcg ccgatcttta ttccggaccg ggacgggcgg ggggtgcgca cgaccttctc
     2401 ctgggggatc gccacgtggc cgcgcgacgg gcaagaggcc gagcggctcc tacagatggc
     2461 ggatcgccgg ctctacgcca tgaagcgcgg ccaacggccc cacgatcccc agtcggcccc
     2521 cgccgcggac tagcgctggg agcaggggag gacctcccct gcgtcgtaga caggggcggc
     2581 ggcaactcgc tgggcgcggg cgaccgcgca gagactgggc cgccggaggg tgttcgatgg
     2641 ccaaggtcct cttggaacag atcaccaaac agttcggcaa cgtcctcgcg gtgaacaacg
     2701 tcacgctcga cgtcccggat aaacagttca cggtgctggt cggaccgtcg gggtgcggga
     2761 agaccacctg cctccggctg gtcgccggac ttgaggaggc cacggccggc aacatctata
     2821 tcggggaacg gctggtcaac gacgtcgctc cgaaggaccg cgacatcgcg atggtcttcc
     2881 agaactacgc gctctacccg cacatgacag tgtacgacaa catggcgttt gggttgcgcc
     2941 tcaggaagaa cgcgcgggcg gagatcgacc ggcgggtcaa ggaagcggcg gagatgctgg
     3001 ggatccagga gctcctctcc cgaaagccca agcagctctc gggcgggcaa cgccagcggg
     3061 tcgccctggg gcgggcgatc gtgcgggagc cccaggtgtt cttgatggac gagccgctca
     3121 gcaacttgga cgccaaactc cgggtgcaga cgcgcgccga gatcaagaag ctgcacgcca
     3181 ggcttcagac gacgacgatc tacgtcaccc acgatcaggt cgaggcgatg accatgggcg
     3241 atcggatcgt ggtgatgaag gacgggctcg ttcagcaggt cgacagcccg ctgagcctgt
     3301 acgagaagcc cgcgaacctg tttgtggccg ggttcatcgg gtcccccgcg atgaacttcc
     3361 tcgaagccaa gctcgccaag caggacggta ggatcgttgt cgacggggac gtgttccggg
     3421 ccgaggtgcc ccaggagttc gtcccg
//
LOCUS       DASQGH010000343         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21233, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000343 DASQGH010000000
VERSION     DASQGH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21233"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VEW91_06690"
     CDS             complement(<1..296)
                     /locus_tag="VEW91_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYM91304.1"
                     /translation="MRSVILEEVPSGRMFIGGEWVGNASGRSFPTFNPATGEVLGQAP
                     LADATDGDRAVRAAHQAFPGWSETPDPERARALRALAAALRSHREELAHLDALNS"
     gene            complement(300..1154)
                     /locus_tag="VEW91_06695"
     CDS             complement(300..1154)
                     /locus_tag="VEW91_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HYM91305.1"
                     /translation="MNRALGTDREPFHWRTAISYKTKDKIVVRGYDLNELTGNVTFAE
                     MAYLVWRGELPPASHGRMLDAILVSMAEHAFSPSSAACRLVASGGVPLHVGVAGGMLA
                     LGSLHGTADRPAALFRTAVERMEREQISLEEAARRVAAETRRAGQRLPGFHHPQHIRD
                     PRTARLLEISDRLGVSKTYVAMARAIEAATEEPWGRRIYLNGPGAVGAICLDMGFDTE
                     LIKGVFLLSRTVSLVAHTYEEVHREKGWRGSANAPIVQPLDLSLQRPEFYDGPRDRAL
                     PRARQGGV"
     gene            complement(1159..1980)
                     /locus_tag="VEW91_06700"
     CDS             complement(1159..1980)
                     /locus_tag="VEW91_06700"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM91306.1"
                     /translation="MARNRPGPSGAPSSGYSRQELMVIEAARHVRNGEVVFVGTGLPL
                     LATTLAQRTHAPGVVCVIESGVIAPQVLPTPISVSDPKVMFGAVKLGTLREVLGCLLQ
                     RGLVDLGFVGGAQIDRYANINSTVIGPPRARERRLPGSGGANDMASHCRRLLIITRHE
                     RRRFPERCDYVTSPGFLDGPDGRKRAGLRPDFSVTVVTDLAVMENDPVTCALRIVKLM
                     PGVTVDAVLAETGFRPGVAPHVGEVVPPSAEDLRVLRDEVDPARVYLKDTEPARR"
     gene            complement(1980..2939)
                     /locus_tag="VEW91_06705"
     CDS             complement(1980..2939)
                     /locus_tag="VEW91_06705"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM91307.1"
                     /translation="MGATLGTLMDTRAIALSTDKVMPAAEAVARFVRPGAVLGMGGQN
                     IGRCPMALAHEIIRQRIGGLTVVGCNLSIAMDELVGAGLVRRCVCGSGNLERFGTTFC
                     WRRGIEAGTLEIEDYSHLAMVTRFLAGEMGVPFMPTRSLLGSDLLPPSGGAAPAGPVV
                     IDNPWKPGEPVVLLPALQPDVSIVHVQRADRMGNLIIEGFATHEPEMIRASRCVIASC
                     EELVPTEEIRRHPERTTIPFIHVSAVVVQRFGAYPTSTYRYYDFDADQVSAYQQAART
                     GGEAVTQYLHTHVWGCATFEEHVARAAAPARLEALRKAMQEVV"
     gene            3008..>3237
                     /locus_tag="VEW91_06710"
     CDS             3008..>3237
                     /locus_tag="VEW91_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYM91308.1"
                     /translation="MNPPPRGRPAPARTRRGGHIPLAPLHTFHLVALHQSVSKAAEWL
                     GISQPAVTQQIRRLERHLGVALFDRTGRRIILT"
BASE COUNT          527 a         1158 c         1054 g          498 t
ORIGIN      
        1 gagttcagcg cgtccaggtg ggcgagctcc tcgcgatgcg accgcagggc cgccgccagg
       61 gcgcgcagcg ccctcgcccg ctcagggtcc ggagtctcgg accaccccgg gaaggcctgg
      121 tgcgccgccc ggaccgctcg atcgccatcg gtggcgtccg cgagcggggc ctggcccagg
      181 acctccccgg tcgccgggtt gaaggtgggg aacgaacgcc cggaggcatt ccccacccat
      241 tccccgccga tgaacatccg gccgctggga acctcctcga ggatcacgga acgcacagtc
      301 tacacgcctc cttggcgggc gcgggggagc gcgcgatcgc gaggaccatc atagaactcg
      361 gggcgctgga gggagaggtc cagcggctgg acgatcgggg cgttggccga tccccgccac
      421 cccttttccc ggtggacctc ctcgtacgta tgcgccacca gcgacaccgt ccgcgagagc
      481 aggaagaccc ccttgatgag ttctgtgtcg aaccccatgt cgaggcagat cgcccccacc
      541 gcccccggcc cgttcaggta gatccggcgc ccccaaggct cttcggtggc cgcttcgatg
      601 gctcgcgcca tggccacgta cgtcttcgag accccgagcc ggtcggagat ctccagcagc
      661 cgggccgtgc gaggatcgcg aatgtgctgc gggtggtgga agcccgggag ccgctgtccg
      721 gcccggcggg tctcggcggc cacccggcgc gccgcctcct ccaggctgat ctgctcccgc
      781 tccatccgtt cgacggcagt ccggaagagg gccgccggac ggtcggcggt gccgtggaga
      841 gacccgaggg ccagcatccc ccccgcgact ccgacgtgga gcggcacgcc cccggaagcg
      901 acaagacggc aggcggccga cgaaggcgaa aacgcgtgct cggccatgct cacgaggatg
      961 gcatccagca tgcggccgtg ggaggcgggc gggagctcgc cacgccagac caggtaggcc
     1021 atctccgcga atgtcacgtt gcctgtgagc tcgttcaggt cgtagccgcg gaccacgatc
     1081 ttgtccttgg tcttgtaact gatcgcggtc cgccagtgaa agggctcccg gtcggtcccg
     1141 agcgcgcggt tcatgccctt acctcctcgc cggctctgtg tccttcaggt agacgcgggc
     1201 agggtccacc tcatcgcgaa gcacccggag gtcctcggcc gatgggggca ccacttcccc
     1261 cacgtgcggc gccacccctg gccggaatcc ggtctccgcc agcacggcat cgacggtgac
     1321 gcccggcatc agcttcacga tgcgcagggc gcaggtcacc ggatcatttt ccatgacggc
     1381 cagatccgtg accaccgtca cgctgaagtc tggcctcagc ccggcccgct tgcgcccatc
     1441 gggaccgtca agaaagcccg ggctggtaac atagtcacac cgctccggaa agcgccgccg
     1501 ctcgtggcgg gtgatgatca acaggcgccg gcagtgcgat gccatatcgt tggccccgcc
     1561 gctgccgggc agccgccgct cccgcgcgcg cggcgggccg atcaccgtgg aattgatgtt
     1621 ggcgtaccgg tcgatctgcg cgccgccgac gaagcccagg tcgaccaacc cccgctggag
     1681 caggcacccc aggacctcgc gcagggtccc cagcttgaca gccccaaaca tcaccttcgg
     1741 atcggagacc gagatgggcg ttgggaggac ctggggcgcg atcacgccgc tctcgatgac
     1801 gcacaccacg ccgggcgcgt gggtgcgttg ggccagggtc gtggccagca gggggagccc
     1861 ggtccccacg aagacgacct ctccgttccg aacgtggcgg gctgcctcga tcaccatcag
     1921 ctcctgccgc gagtagccgg aggatggcgc gccggacggt cccggccggt tccgagccat
     1981 cagaccacct cctgcatcgc cttgcgcagg gcttccagcc gcgcgggcgc ggcggcacgc
     2041 gcgacgtgct cctcaaacgt cgcgcacccc cagacgtggg tgtgcaggta ctgcgttacc
     2101 gcctcgcccc cggttcgcgc cgcctgctgg tacgcgctga cctggtcggc atcgaaatcg
     2161 tagtaccggt aggtggacgt gggatacgct ccgaatcgct ggaccaccac cgcgctgaca
     2221 tggataaagg ggatggtcgt ccgctccggg tgcctccgga tctcctcggt cgggacaagt
     2281 tcctcgcagc tggcgatcac gcaccgggag gcccggatca tctcgggttc gtgggtggca
     2341 aatccctcaa tgatcaggtt ccccatccga tcggcccgct gaacgtgcac gatgctcaca
     2401 tccggttgga gtgccggcag cagcaccacg ggttcccccg gcttccacgg attgtctatc
     2461 accaccggtc ccgcaggggc cgctccgccc gaggggggca gcaggtccga ccccagcagg
     2521 cttcgggtgg gcatgaacgg gacgcccatc tcacctgcaa gaaatcgggt caccatcgcg
     2581 aggtggctgt agtcctcaat ctcgagcgtc cccgcttcga ttccgcgtct ccagcagaac
     2641 gtggttccga atcgctccag gttgcccgac ccgcagacac accgcctgac gagtccggcc
     2701 ccgacgagct cgtccatcgc aatcgacagg ttgcagccga caacggtcag gccgccgatg
     2761 cgctgacgga tgatctcgtg ggccagcgcc atcgggcagc gtccaatgtt ctggccgccc
     2821 atgcccaata cggccccagg gcggacaaac cgagcgaccg cttccgctgc gggcatgacc
     2881 ttgtcggtgc tcagagctat ggcgcgggta tccatcagcg tccccagggt tgctcccatg
     2941 gtagggtagg cgctatcata atgtcaaata agggcaggta catgaattca ttaggtttgc
     3001 ttataacatg aacccacccc cgcgcgggcg tcccgcaccc gcgcgaacgc ggcgaggggg
     3061 ccacatcccg cttgcccccc tccatacatt tcacctcgtc gcgctgcatc agagcgtcag
     3121 caaggccgca gaatggctcg ggatcagcca gcccgcggtc acccaacaga tccgccgcct
     3181 ggaacggcac ctgggggttg ccctgttcga ccgcacaggc cgccggatca tcttgac
//
LOCUS       DASQGH010000344         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24244, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000344 DASQGH010000000
VERSION     DASQGH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24244"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VEW91_06715"
     CDS             <1..223
                     /locus_tag="VEW91_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91309.1"
                     /translation="DELTARTGGPVLNVFVPATPNPMSGYLVMLPERDIQFLPMSVQD
                     GLKLVISGGIATPGPQRATQPRRGEGAAS"
     gene            325..927
                     /locus_tag="VEW91_06720"
     CDS             325..927
                     /locus_tag="VEW91_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYM91310.1"
                     /translation="MGVLVVTGWAAAAWGAAAAAPERYVIVPSESQVIYRVGEEFLEQ
                     GTQFNVAVGTTNAVHGEVLIDHADPRRSRVGPVTVDISRFQSDQPRRDNAIRRRWLES
                     ERYPTAVFTPTAIHGLPDAYTLGHEILAQITGTLRIRDVTRVTTFATRVRLDGDTLTG
                     VATAMIHMTDFGFDPPSILGFVKAENEATLELHFLARRAG"
     gene            967..1896
                     /locus_tag="VEW91_06725"
     CDS             967..1896
                     /locus_tag="VEW91_06725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYM91311.1"
                     /translation="MTDLERYILDEWTEDYRVGRLVRREFLRRIAVFSGGAAAGLAVL
                     ANRGVAASALEIAEAAAGPVPTAAQGAGVTVAPDDPAIETRSVSFPSDGTTTLAYLAL
                     PRNKPKAPGVSVVHENRGLLEHHKDVTRRLAKAGYAALAVDLASPGGGTDRFQDSAQV
                     SGLIGRTPPDQLVAMLNAGVGYLQGLPAVRPDRQGTVGFCFGGGVVWRLATRNPALRA
                     AVPFYGPNPPLEDVPKIRAAVLALYGSLDTFVTPGIAAIRQALDQAKVTYEMHIYEGA
                     NHAFFNDTAGSYNPEAAKDAWARTLGWFARWLS"
     gene            1893..2387
                     /locus_tag="VEW91_06730"
     CDS             1893..2387
                     /locus_tag="VEW91_06730"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HYM91312.1"
                     /translation="MSAAKRARRPRPSPRPRKRRPGVHAQRLPAQYTRLLRAAARARP
                     RAYAPYSQFPVGAAVLATDGSIYAGCNVENASYGLALCAERVAIHTAVASGRRRLTAL
                     AVVGPAGITLTPCGACRQVMEEFGLQTVIIASPRGAPAVVSLPALLPIPFTRGWTRGR
                     HAGL"
     gene            2374..3216
                     /gene="recO"
                     /locus_tag="VEW91_06735"
     CDS             2374..3216
                     /gene="recO"
                     /locus_tag="VEW91_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYM91313.1"
                     /translation="MPVYKAEAIVLRQQALGESDRIVTLFTREHGKLRAAAKGVRRPT
                     SRLAGRIEPFTHAQLMLARGRTLDVIAQAEIIRTFAGVRKDLIRAAYAAYVVELIDRG
                     LPERDRHEDVFGLAGDALEQLDGGRAEDAEVTTLWFGLRLAGLLGYQPETAACVECGR
                     RLPRARGAAEAWAFSPSSGGALCPGCRPHDPQAMNASCGALGACEYLATAPKGQWARL
                     RISTAQRAELARLVQAHLEHRLEVRFRAPQVIRRLAQASPPELARAAHEQPGVGAPPG
                     GRPA"
BASE COUNT          455 a         1167 c         1225 g          468 t
ORIGIN      
        1 ggatgagctc accgcgcgga cgggggggcc ggtcctgaat gtgttcgtcc cggccacgcc
       61 caatccgatg tcgggctacc tggtcatgct gcccgagcgg gacattcagt ttctccccat
      121 gagcgtccag gacgggctca agctggtcat ctccgggggc atcgccaccc cggggcccca
      181 gcgggcgacc cagccccggc gcggtgaagg agcagcgtcg tagcatgcgc gcgcgcggcg
      241 gaagagcggt gggctggtgt tggtcccatc gggatgcgcc cgcgccgcag gaagcgagga
      301 gcgcccctga cgcgaccgtg gtgggtgggg gtccttgtcg tgacggggtg ggcggccgcc
      361 gcgtggggcg ccgcagccgc cgcgcctgag cggtacgtga tcgtcccgtc ggagtcccag
      421 gtgatctacc gggtcggcga ggaattcctt gaacaaggga cccagttcaa cgtcgcggtc
      481 ggcaccacga atgcggtcca cggcgaggtg ctgatcgacc acgccgatcc ccgccgcagc
      541 cgcgtcgggc cggtcaccgt ggacatcagc cggttccagt ccgatcaacc gcgccgggac
      601 aacgcgatcc gcagacggtg gctcgagtcg gagcggtacc ccaccgcggt cttcaccccg
      661 accgcgatcc acgggctgcc ggacgcctac accctggggc acgaaatcct cgcgcagatc
      721 acggggaccc tccggatccg cgacgtcacg agggtgacca cattcgcgac acgggtccga
      781 ctcgatggcg acacgctcac gggcgtggcc accgcgatga tccacatgac agactttggg
      841 ttcgatccac cgtcgatcct gggcttcgta aaggcggaga acgaggcgac gctggagttg
      901 cacttcctgg cccgccgcgc ggggtgagca gccgctcgtg cgcgacgtca cgtcgagggg
      961 ggagcgatga ccgacctgga gcgctatatc cttgacgagt ggaccgagga ctaccgcgtg
     1021 gggcgccttg tgcggcggga gttcctgagg cgcatcgcgg tgttttcggg gggcgcggcc
     1081 gccggcctgg ccgtgctcgc gaaccgcggg gttgcggcct cggccttgga gatcgctgag
     1141 gcggcggccg gaccggtccc gacggcggcc cagggcgcag gggtgacggt cgcgccggac
     1201 gaccccgcga tcgagacccg ctccgtgtcc tttccctccg atgggacgac cacactggca
     1261 tacctcgctc tgccgcggaa caagccgaag gcgccggggg tgagcgtcgt ccacgagaac
     1321 cgcggcctcc tcgagcacca caaggacgtg acccggcggc tcgccaaggc gggctacgcc
     1381 gcgctggccg tggatctcgc ctcgccgggg ggagggacgg acaggtttca ggattccgcg
     1441 caggtgtcgg ggttgatcgg ccgcacgccg cccgaccagc tcgtcgccat gctcaacgcc
     1501 ggcgtggggt acctccaggg gctccccgcg gtgcgacccg accgccaggg gacggtgggg
     1561 ttctgcttcg ggggcggagt ggtctggagg ctcgcgaccc gcaaccccgc cctccgggcc
     1621 gcggtgccgt tctatggccc caaccccccg ctcgaagacg tgccgaagat ccgcgcggcc
     1681 gtgctcgcgc tctacggctc gctcgatacg ttcgtcaccc ccggcatcgc ggcgatccgg
     1741 caggcgctcg atcaggccaa ggttacctac gagatgcata tctacgaggg cgcgaaccac
     1801 gcgttcttca acgacaccgc tgggagctac aatccggagg cggcgaagga cgcgtgggcc
     1861 cggacgcttg gctggttcgc ccggtggttg tcatgagcgc ggcgaagcgc gcccgccgtc
     1921 cccggccgtc cccgcggccg cgcaagcgcc ggcccggggt gcatgcccag cgcctcccgg
     1981 cgcagtacac gcgcctgctt cgcgccgctg cgcgcgcccg cccccgcgcg tatgcgccgt
     2041 actcccagtt cccggtgggg gccgcggtgc tggcgaccga cggatcgatc tatgcggggt
     2101 gcaacgtcga gaacgcctcc tacggtctgg ccctgtgcgc ggagcgggtg gcgattcata
     2161 ccgcggtggc gagcggccgc cggcggctga ccgcgctcgc ggtggtgggg cccgccggga
     2221 tcaccctcac gccgtgcggc gcctgccgcc aggtgatgga ggagttcggc ttgcagaccg
     2281 tgatcatcgc ctccccacgg ggcgcccccg ccgtggtctc gctcccggcc ctcctcccga
     2341 tcccgttcac ccggggatgg acacggggcc gccatgccgg tttataaggc tgaagcgatc
     2401 gtactccgcc agcaggcgct cggggaatcc gacaggatcg tcacgctgtt cacccgggag
     2461 cacggcaagc tccgggccgc cgccaagggg gtgcgccggc cgaccagccg gcttgccggt
     2521 cggatcgagc cgtttacgca cgcgcagctg atgctggccc gcgggcggac gctggacgtc
     2581 atcgcgcagg ccgagatcat caggacgttc gccggggttc gcaaggacct gatccgcgcc
     2641 gcgtatgcgg cctacgtggt ggagctcatt gaccgcggcc tccccgagcg cgaccggcac
     2701 gaggacgtct tcggcctggc gggggacgcg ctggagcagc tggacggggg ccgggcggag
     2761 gacgcggagg taacgacgct ctggtttggc ctgcgcctgg ccgggcttct cggctaccag
     2821 cccgagaccg cggcgtgcgt cgagtgcggg cgccgcctcc cccgcgcgcg gggcgccgcg
     2881 gaggcctggg ccttcagtcc ctccagcggt ggagccctct gcccagggtg ccggccccac
     2941 gatccccagg cgatgaacgc gtcgtgcgga gcgctcgggg cgtgcgagta cctcgccacc
     3001 gcgcccaagg ggcagtgggc ccgcctccgg atctcgaccg cccagcgggc cgagctggcc
     3061 aggctggttc aggctcacct ggagcaccgg ctcgaggtca ggttccgcgc gccccaggtg
     3121 atcaggcggc tcgcccaggc gtccccgccg gagctggcgc gcgcagcgca cgagcagccg
     3181 ggtgtaggcg cgccccctgg cggccggccc gcgtgataag atagacccgc ggaggtggtc
     3241 cgatggagat cgatctggag cgggcgaagc gcgcgatcgc ccagttcggg tcggcgattg
     3301 atgagatccg gcgcg
//
LOCUS       DASQGH010000345         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32722, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000345 DASQGH010000000
VERSION     DASQGH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32722"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VEW91_06740"
     CDS             <1..191
                     /locus_tag="VEW91_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HYM91314.1"
                     /translation="LPDRRRAIERGIALARPGDIVVIAGKGHETYQIIGDRAEPFDDR
                     EIAREVLRASRARGGAPR"
     gene            188..1606
                     /gene="murF"
                     /locus_tag="VEW91_06745"
     CDS             188..1606
                     /gene="murF"
                     /locus_tag="VEW91_06745"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HYM91315.1"
                     /translation="MTSDSPAALTAAEVADIVGGRLEHGAGAAVLRGAAVDSRTVAPG
                     MLFVTLVGAQADGHRYAGDAFRRGASGALVSQPLAEVPPGAPVIRVPDTLRALHRLAR
                     AVRDRRRLRVVGITGSVGKTSTKEMAAGVAGRVFRTARSPENWTTEIGIPLVLTNLPE
                     DREVVVLEMAMRGPGQIRELVEIAHPEIGVVTNVGESHLDFFPSRDALASAKGELIEG
                     LPADGCAILNADDPLAWGLRGRARARVLSFGLERGEVRAEAVHPLALGGSTFLLKTPG
                     GQAPVTLHVPGRHSVRNALAAAAVGVALGIGAEVIASALGEAAPLPMRLELIRLGRTA
                     LLSDVYNSSPQSVLAALDTLDEIEGSPRVAVLGDMKELGAHSAEAHRRVGREAARRRI
                     DLLVAFGPLAAELAAGAREAGGPRVVHTERLDDVVGLLQQTLVPGSVALIKGSRAMAM
                     ERITAALMRPEPTGAPGRGGSA"
     gene            1603..2568
                     /gene="mraY"
                     /locus_tag="VEW91_06750"
     CDS             1603..2568
                     /gene="mraY"
                     /locus_tag="VEW91_06750"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYM91316.1"
                     /translation="MTPALAAGALAATLTLVGGGPLVGWLRRAGAVKSIREEAPKRHG
                     EKAGTPTMGGLVIIGAVLAATLIVGVAARRVPPALLVALGVVAAFSGIGAADDILGIL
                     RRRNLGLRVRERLGLQLPVALLWGVYVASQMHLGSWIGVPGTHLRVDLGWGYTLFCML
                     FVVGMANAVNLTDGLDGLAAGSVAIAATALAILAARSGAVPASVLCAALAGATLGFLW
                     FNAHPARVFMGDLGSQGCGALLAVAGILAKMELLVLIIGGLFVWEALSVFLQVAAFKT
                     TGRRIFRMSPFHHHCELLGWTETQTVVRFWVCGALLAALGVGLAG"
     gene            2565..4058
                     /gene="murD"
                     /locus_tag="VEW91_06755"
     CDS             2565..4058
                     /gene="murD"
                     /locus_tag="VEW91_06755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYM91317.1"
                     /translation="MIAEVVARLRGRRIHVVGLSGTEGAAVTEFLLEQGVTSITAHDL
                     QTEEGFAAAFRRYHRWMDPAAQEAAIRRLLHAPIQIRFRDRYLEGIAAAELIYAGQAW
                     FRYPENAPVARARDAGVPLSSMTELFFETVPCPILGVTGTNGKFTVVQLAAEMLAQSG
                     RRTFVSGNDRTHIPILYRLREVSREAILVLEISNRQLVGLRHSPQIAVITNLAPHHLD
                     DHGSFEAYVRVKAGILAHQGAGDRAILNADDPPTWDLAQSAPGAVLPFSRLREVGEGA
                     CLVADRITVRLGGREDRLCGVAELAVPGTHMVENALAAALAARCAGAAPEAVTSVLRA
                     FRGLPYRMRFVAEVDGIRFYEDSLATNPAAAAAAIRAFDRPLVLIAGGQRPGGTADDF
                     RPMAEALQERSVRAVLLIGAMAPHIAEALAAAGPGAPVIHCGTLEAAVHRAREVARPG
                     DVVTLSPGCESFDQFRDYRERGDRYIGLVTAPDQAAAGAAGGSGRWT"
BASE COUNT          482 a         1422 c         1609 g          582 t
ORIGIN      
        1 cgctccccga ccggcgtcgg gcgatcgagc gcgggatcgc cctggcccgt cccggggaca
       61 tcgtcgtgat cgccggcaag gggcacgaga cctaccagat catcggcgac cgcgccgaac
      121 cgttcgacga ccgcgagatc gcccgcgagg tgctgcgggc cagccgggcc cggggagggg
      181 cgccgcggtg accagcgact cgcccgccgc gttgaccgcg gccgaggtgg cggacatcgt
      241 cggcggccgg ctcgagcacg gggcgggtgc ggccgtgctg cggggggccg ccgtcgacag
      301 ccggacggtg gcgccgggca tgctcttcgt caccctggtc ggcgcccagg ccgatgggca
      361 ccggtacgcc ggcgacgcgt tccggcgcgg cgcctccggg gcgctcgtct cgcagcccct
      421 ggccgaggtc ccgcctgggg cgccggtcat ccgggtcccg gacacgctcc gcgccctcca
      481 ccggctggcc cgcgccgtgc gggaccggcg ccggctccgg gtcgtcggca tcacggggag
      541 cgtggggaag acgtcgacca aagagatggc ggcgggcgtg gctgggcggg tgtttcggac
      601 cgcccgatcg ccggagaact ggaccaccga gatcggcatc cccctcgtgc tcaccaacct
      661 gccggaggat cgggaggtgg tggtgctcga gatggcgatg cggggtcccg ggcagatccg
      721 cgaactcgtg gagatcgcgc accccgagat cggggtcgtg accaacgtgg gcgagtccca
      781 cctcgacttc tttcccagcc gcgacgcgct cgcctccgcc aagggcgagt tgatcgaggg
      841 cttgcccgcg gacggctgcg cgatcctcaa cgcggatgac ccgctggcgt gggggctccg
      901 cggtcgcgcc cgcgcgcggg tcctctcctt cgggctcgag cggggggagg tgcgggccga
      961 ggccgtgcat ccgctcgcgc tcgggggaag cacgtttctc ctcaagaccc cggggggcca
     1021 ggcaccggtg acgctccatg tgccgggccg ccactcggtc cgcaacgcgc tggcggccgc
     1081 cgcggtgggg gtcgcgctgg ggatcggcgc cgaggtcatc gcctccgcct tgggcgaggc
     1141 cgcgccgctg ccgatgcgat tggagctcat tcgcctcgga cggacggccc tcctgagcga
     1201 tgtctacaac agcagcccgc aatccgtgct ggcggcgctc gacacgctgg acgagatcga
     1261 gggctccccg agggtcgcgg tgctgggcga catgaaagag ctcggcgcgc actccgcgga
     1321 ggcccaccgg cgggtcggcc gcgaagcggc ccgccggcgc atcgacctcc tggtggcgtt
     1381 cggcccgctc gccgcggaac tggcggcggg ggcgcgtgaa gccggggggc cgcgggtcgt
     1441 gcacaccgag cgcctcgacg acgtggtggg cctgctccag cagacgctcg tccccgggtc
     1501 ggtggcgctg atcaagggct cgcgggcgat ggcgatggag cggatcaccg ccgccctcat
     1561 gcgccccgag ccgaccgggg cgcccggccg gggagggagc gcgtgacgcc ggcgttggcg
     1621 gccggggccc tcgcggccac gctgaccctg gtgggtggcg gcccgctggt gggctggctc
     1681 cggcgcgcgg gagcggtgaa gtcgatccgc gaggaggcgc ccaagcgcca cggcgaaaaa
     1741 gcggggacgc ccacgatggg cggcctggtg atcatcgggg cggtgctcgc ggccaccctg
     1801 atcgtcggag tggccgcgcg gcgcgtcccg ccggcgctcc tggtcgccct cggggtcgtg
     1861 gccgcgttca gcggcatcgg cgcggcggac gacatcctgg ggatcctgcg ccgccggaac
     1921 ctggggttgc gggtccggga gcggctgggg ctccagctcc cggtcgcgct gttgtggggc
     1981 gtctatgtcg cctcgcagat gcacctcggc tcctggattg gggtgccggg gacgcatctg
     2041 cgggtggacc tgggctgggg ctacacgctg ttctgcatgc tcttcgtcgt cggcatggcg
     2101 aacgcggtca acctgaccga cggcctcgac ggcctggccg ccgggagcgt cgcgatcgcc
     2161 gccacggcgc ttgcgatcct cgcggcgcgc tcgggcgccg tccccgccag cgtgctgtgc
     2221 gcggcgctcg cgggggcgac gctcggattt ctctggttca atgcgcaccc ggccagggtg
     2281 ttcatgggcg acctgggatc gcagggatgc ggcgcgctcc tcgcggtggc ggggatcctg
     2341 gccaagatgg agctcctggt gctcatcatc gggggactgt tcgtctggga ggccctctcc
     2401 gtgttcctcc aggtcgcggc cttcaagacg accggccggc ggatcttccg gatgagcccg
     2461 ttccatcatc attgcgagct gctgggatgg accgagacgc agaccgtcgt gcggttctgg
     2521 gtctgcggcg cgctgctggc ggcgctcggc gtggggctcg cgggatgatc gccgaggtgg
     2581 tcgcgcggct gcgcggcaga cgcatccacg tggtcgggct ctcgggcacc gaaggcgctg
     2641 cggtcacgga gtttctgctc gagcaggggg tcacgtcgat caccgcgcac gacctgcaga
     2701 cggaggaggg gttcgcggcc gcgttcaggc ggtatcaccg gtggatggat cccgccgcgc
     2761 aggaggccgc gatccgccgg ctcctccacg ccccgatcca gatccgcttc cgcgaccggt
     2821 acctggaggg catcgccgcg gcggagctga tctacgcggg gcaggcctgg ttccgctatc
     2881 ccgagaacgc cccggtcgcc cgggcgcgcg atgccggggt gcccctctcc agcatgaccg
     2941 agctcttctt tgagacggtc ccctgcccga tcctcggggt cacggggacc aacggcaagt
     3001 tcacggtcgt gcagctggcg gccgagatgc tcgcccaaag cggccggcgg accttcgtga
     3061 gcgggaacga ccggacgcac atccccatcc tctaccggct gcgtgaggtg tcgcgcgagg
     3121 cgatcctggt gctggagatc agcaaccggc agctcgtggg gctgcgccac agcccgcaga
     3181 tcgccgtgat cacgaacctc gcgccgcacc acctggacga tcacggctcg ttcgaggcct
     3241 acgtccgggt caaggccggg atcctcgcgc atcaaggggc gggcgatcgg gcgattctca
     3301 acgccgatga tcccccgacg tgggacctgg cgcagagcgc gccgggcgcc gtgctgccgt
     3361 tcagccgcct gcgggaggtg ggggagggcg cgtgcctcgt ggcggaccgg atcacggtgc
     3421 gcctcggcgg gcgggaggac cggctgtgcg gggtggccga gctcgcggtg cccgggacgc
     3481 acatggtgga gaacgcgctc gccgccgcgc tggccgcccg gtgcgccggg gcggcgccgg
     3541 aggcggtgac gtcggtcctg cgggcgtttc gcggcctgcc gtaccggatg cggttcgtgg
     3601 ccgaggtcga tgggatccgg ttctatgagg actcgctcgc cacgaacccc gccgccgcgg
     3661 cggccgcgat ccgcgcgttc gatcgcccgc tcgtcctcat cgccggcggc cagcggccgg
     3721 gagggaccgc ggacgatttc cgtccgatgg cggaggcgct ccaggagcgc tccgtccgcg
     3781 ccgtgctcct gatcggggcg atggcccccc acatcgcgga ggcgctcgcg gcggcggggc
     3841 cgggggcccc ggtgatccat tgcggcacgc tggaggccgc ggtccacagg gcgcgcgagg
     3901 tcgcacggcc cggagatgtc gtgacgctct cccccggctg tgagagcttc gatcagtttc
     3961 gcgactaccg ggagcgcggg gaccggtaca tcggcctcgt gaccgcaccg gaccaggcgg
     4021 cggcgggtgc cgccggcggg agcgggcgat ggacgtgatc caccgccggg ccgcggcctg
     4081 gggggtgttc gtcgt
//
LOCUS       DASQGH010000346         7418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12929, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000346 DASQGH010000000
VERSION     DASQGH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12929"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VEW91_06760"
     CDS             <1..351
                     /locus_tag="VEW91_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HYM91318.1"
                     /translation="ATDGVDYAFTRVKGAANILFSGTGFFIDTFTCLRPEGILWLHGY
                     GNVFEVTLGPNEQIDIEPGGWGYKDRTVQMQTIFQKFSTGVFASAGQIIWNRFTGPGR
                     IALQSMYVHLPTEG"
     gene            complement(376..1317)
                     /locus_tag="VEW91_06765"
     CDS             complement(376..1317)
                     /locus_tag="VEW91_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYM91319.1"
                     /translation="MKHLPMGQTGVKVSAIGLGTNSFGGRADRATSVAILHRAIDAGI
                     TLIDTANIYTATASETIIGEGLRGRRHQVLLATKAGMKTGEGPNDGGASRHHLMRELD
                     ASLRRLQTDYIDLYQIHTWDPATPLEETLRTLDDMVGSGRVRYIGTSNYTAWQVTKAL
                     WVSDRRGFVRFETVQPSYSLADRAVERELVPLCQDQGVGLIAYFPLAGGILTGKYRRG
                     QDPPKGSRAVTQPQFAKRISDQGLRLAEDVAALASEVHCTPSQLALAWLLRQPAVCSA
                     IAGATSVAQLEENLGALEVTLSGEVLARLDEISRPFV"
     gene            1434..2294
                     /locus_tag="VEW91_06770"
     CDS             1434..2294
                     /locus_tag="VEW91_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYM91320.1"
                     /translation="MPELPFVAVLVENLRAQVEGRTIDDVLVRSVYVLKTFDPPITDI
                     KTRRITRIERRGKLLVFHLSGGLAMVVHLRRNGRLQILPRGKGRTARDLAITFALDGG
                     ADLQLIELGPKKAASVWLFPAGHEPTAEPLAGLGLEPLAGEFTREALAGLLGEERTHL
                     KRFLTLQRHVVGIGNAFADEILWEARLSPQAMTNALDDAEVARLHGAIRKVLEEAVAA
                     HRKEFAGALPMREPVHLLRVHRHGKEPCPRCGTAIAVIYYEDRETYYCPTCQAGGKVY
                     ADRRRSRLLR"
     gene            2338..3300
                     /locus_tag="VEW91_06775"
     CDS             2338..3300
                     /locus_tag="VEW91_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HYM91321.1"
                     /translation="MGAAVVVLVVGAIGVAGGAQSPAPPLREYAVPRGSHPHDVAPAP
                     DGSVWYTAQTAGELGRLDPATGRIRGIKLGEGSAPHGVIVGPDGAAWVTDSGLNAIVR
                     VSPTTNEVRRFPLPGDHEAANLNSAAFDRQGTLWFTGQSGAYGRVAPATGRVDVWDAP
                     RGPGPYGITATPDGAIYFVSLAGSYLARIDTTSGAATVLQPPTSGQGARRVWSDRRGR
                     LWVSEWNASSVALYDPTSGRWREWRLPGERPMPYAVYVDDQDMVWLTDWGANALVRFD
                     PRRETFEAFPFPTAGASVRQILGRPGEVWGAESSVDRLVVWRRR"
     gene            3339..3806
                     /locus_tag="VEW91_06780"
     CDS             3339..3806
                     /locus_tag="VEW91_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM91322.1"
                     /translation="MLAIRAELVRFLDDTAGRWNWAHKGLREAIKDLDPDEAVWKPPG
                     GHSVGEQIRHVAYWKRYILHRLQGKRPRMRQAWPAPGRTAAALRRAKADLGALHRELR
                     TTVIGLDPDALFERRGQRGTIARLLMGGAAHESYHIGQILLTRKLYRRRHRVR"
     gene            complement(3791..5017)
                     /locus_tag="VEW91_06785"
     CDS             complement(3791..5017)
                     /locus_tag="VEW91_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HYM91323.1"
                     /translation="MGSVLLRGGQALHLGGVPNNTAVDIGIGADGRVALAGRWQGQSR
                     FDEVIDLRGARLCPAWIDLHVHCYYGGTWLSVRPEQVGPSTGVGLAVDCGSAGEANFA
                     GLREFIIAPAPFPILAFLNISTIGLVAANRVSELIGDPVLDPERTAQVVEAHRDLIKG
                     IKVRASNQVVREWGMTPVRVAKALARAVRLPLVVHIGEAPPTLDEILDILEPGDMITH
                     CFTGRITTSIMRHEPYFRRCETMAAAGLVLDVGHGQGSFHYPTARRAVERGLLPTTIS
                     TDLHIGNVHGPVWDLATTMSKMVMLGVSVEDAVERATVAPARFLGLEDWGRAAIGAPA
                     RFTAFEIEDGEEPLPDSYGNIEVVRRFFEPRYTVVGAAVVPAARNTARGPRSVSIEAA
                     SDGAPSTGSLRPQRTR"
     gene            complement(5069..5953)
                     /locus_tag="VEW91_06790"
     CDS             complement(5069..5953)
                     /locus_tag="VEW91_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91324.1"
                     /translation="MAQGTLTSPLVSGARPGAAALLWRMIRRRRITGVGAALILLVVL
                     GAAAAPLLSPYDPVALNVPDRLQGPRDAHPFGTDEFGRDILSRTLYGARLSLLVGALV
                     TSIATTAGVLAGLAAGSSARADRILMRIMDGLMAFPDVLLAIGLMAALGPSARNVVLS
                     LGFVYTPRIARVVRAAVLVVIRLEYVEAARAVGASSARILARHILVNCASPVIVQATF
                     ITAYAMLGEAALSFLGVGVPPQIPTWGGIISAGQVYLRQAPWISIFPGVAIILSVLAL
                     NLLGDGLRDFLDPRLRHS"
     gene            complement(5961..6902)
                     /locus_tag="VEW91_06795"
     CDS             complement(5961..6902)
                     /locus_tag="VEW91_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91325.1"
                     /translation="MLSYLIGRLLALVPVLLVVAAVVFLLIHLTPGDPARVLLGQDAT
                     PEQVQALRHDLGLDRPLVVQFGFWFGRAIRGDLGLSLFQHIPVTQDILQHASPTVMLS
                     LLAIAVALLIGIPAGVVSAVFRNSWLDQGSLALAMLGAAVPSFWLGLSLIVVFAVNLG
                     WLPSSGYRSPTEGVGLSLHYLLLPALALGLPNSSLIIRFTRSSLLDVIGNDYIRTARA
                     KGVNERVVIFHHAFRNALVPILTVVGLTFAALMGGAVVTETVFSLPGVGQLVVSSVLR
                     RDYPVIQGVILLVATIYVLINLAVDLLYFLVDPRVKY"
     gene            complement(6934..>7418)
                     /locus_tag="VEW91_06800"
     CDS             complement(6934..>7418)
                     /locus_tag="VEW91_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91326.1"
                     /translation="LMKEAGYNGEPVRWLTTQQYDYMFKSTVVAFAQLQHVGFKVDMQ
                     VLEWAGVLDHRGKPADYDLFTTSHGFVPDPSLITVFNPGYPGWWDSPEKNKLFDEFNR
                     TSDQGARAGIWSKLQQLVYQQAPLVRPGEYYTLQMWAKGVQGFHASYFLVLWNVQPAK
                     "
BASE COUNT         1191 a         2487 c         2690 g         1050 t
ORIGIN      
        1 gccacggacg gcgtggacta tgcgttcacc cgggtgaagg gagccgccaa catcctcttc
       61 agcgggaccg ggttcttcat cgacaccttc acctgcctcc gcccggaagg gatcctctgg
      121 ctgcacggct acggcaacgt cttcgaggtc acgctcggcc caaacgagca gatcgacatc
      181 gagccgggcg ggtggggcta caaggaccgc accgtgcaga tgcagacgat cttccagaag
      241 ttctcgaccg gggtgttcgc gagcgcgggc cagatcatct ggaaccgctt tacggggccc
      301 gggcggatcg cgctgcagtc gatgtacgtg cacctgccga ccgagggatg aaagcggcat
      361 cgaccacaat cttccctaca cgaacgggcg gctgatctcg tcgagtcggg cgaggacctc
      421 gccggacaga gtgacctcca gggcgcccag gttctcttcc agttgggcga ccgaggtggc
      481 gccggcgatg gcgctgcaca ccgccggctg ccggagcagc caggccagcg ccagctgcga
      541 cggcgtgcag tgcacctcgc tcgcgagggc ggcgacgtct tccgccagcc ggaggccctg
      601 gtcgctgatg cgcttggcga actggggttg ggttaccgcg cgggagcctt tgggcgggtc
      661 ctgcccccgc cggtacttcc cggtcaggat gccccccgcg agcgggaagt acgcgatcaa
      721 cccgacgccc tggtcctggc agagggggac gagttcgcgc tcgaccgcgc ggtcggccag
      781 cgagtaactc ggctggacgg tctcgaagcg gacaaagccg cgccggtcgc tgacccagag
      841 cgccttcgtg acctgccagg cggtgtagtt ggacgtcccg atgtaccgga ccctgccgga
      901 tcccaccatg tcgtccaggg tcctgagcgt ctcctccagc ggcgttgcgg gatcccaggt
      961 gtggatctga tagaggtcga tgtaatccgt ctggaggcgg cgcaggctgg cgtccagttc
     1021 ccgcatcagg tggtggcggg aggccccgcc gtcgttgggg ccctccccgg ttttcattcc
     1081 ggctttggtc gccagcagga cctgatggcg ccggccccgg agcccctcgc cgatgatcgt
     1141 ctccgacgcg gtcgcggtat agatgttggc cgtgtcgatc agggtgatcc ccgcatcgat
     1201 cgcccggtgc aggatggcca cggatgtcgc gcggtcggcg cggccgccga acgaattcgt
     1261 gccgagcccg atcgccgaca ccttgacgcc cgtctggccc atcggcagat gcttcatgca
     1321 caagctcctc atgcagagtg ggaacaccgg tcagttcgga gaccgtgcgc gggtctcctg
     1381 cggccgccgc ggcgatagga gaatccaaac cgcgcggcgc atactccggc gggatgccgg
     1441 agctgccgtt tgtcgcggtg ctggtcgaga acctgagggc tcaggtagaa ggccgcacga
     1501 tcgacgacgt gctcgtccgg agcgtctacg tcctcaagac cttcgatcct ccgatcacgg
     1561 acattaagac ccggcggatc acccgcatcg agcgaagggg caagctcctg gtgtttcacc
     1621 tgagcggggg gctcgccatg gtcgtgcatc tcaggcgcaa cggccggctg cagatccttc
     1681 cgcgcgggaa ggggcggacg gcgcgggatc tggcgatcac ttttgcgctc gacggcgggg
     1741 ccgatctcca gctgatcgaa ctgggcccca agaaggccgc ctcggtgtgg ctgttccccg
     1801 ccggacacga acccacggcc gaaccgctgg ccgggctcgg actcgagccg ctggcaggcg
     1861 agttcacgcg cgaggcgctg gccggcctgc tcggtgagga acggacgcac ctgaagcgct
     1921 tcctcaccct ccagcgccac gtggtgggga tcggcaacgc gttcgccgac gagatcctgt
     1981 gggaggcccg gctgtcgccc caggcgatga cgaacgcgct ggacgacgcc gaggtcgcgc
     2041 ggctgcacgg agccatccgg aaggtgctgg aggaagccgt cgcagcgcac cggaaggagt
     2101 tcgccggcgc gctgccgatg cgcgagccgg tccaccttct gcgcgtccac cggcacggca
     2161 aagagccctg cccgcggtgc ggcacggcca tcgcggtcat ctactacgag gaccgggaga
     2221 cctactactg tcccacatgc caggcgggag ggaaggtcta tgccgatcgg aggcggtcga
     2281 ggctgcttag gtgacccggg ccatggcagg ccctggcggc ggctaggaat agcgcggatg
     2341 ggggcggcgg tggtggtcct cgtggtcggg gcgatcgggg tcgccggagg agcgcagagc
     2401 cccgcgccgc cactgcgcga gtacgcggtc ccgcgcggct cgcacccgca cgacgtggcg
     2461 ccggcgcccg acggcagcgt ctggtacacg gcgcagaccg cgggtgagct gggacgcctg
     2521 gatccggcaa ccggcaggat ccgggggatc aagctgggcg aaggatcggc cccccacggc
     2581 gtcatcgtgg gtccggacgg cgcggcatgg gtcacagata gcggcctcaa cgccatcgtg
     2641 cgggtgtcgc cgacgaccaa tgaagtgcgc cggtttccac tgccggggga tcatgaggcg
     2701 gccaatctca actcggcggc gttcgaccgg caggggaccc tctggttcac cgggcagagc
     2761 ggggcctatg gccgggtggc gcccgccacg gggcgggtgg acgtttggga tgccccacgg
     2821 ggccccgggc cgtacgggat cacagccacg ccggacgggg cgatttattt tgtctccctc
     2881 gcgggcagct acctcgcccg catcgacacc acgagcggcg ccgccacggt gctgcagccg
     2941 cccacctccg gccagggcgc gcgccgcgtg tggtcggatc gccggggccg cctctgggtg
     3001 agcgagtgga acgccagcag tgtcgcgctc tacgatccca caagcggccg gtggagggag
     3061 tggcgccttc cgggcgagcg gccgatgccc tacgcggtgt acgtcgacga ccaggacatg
     3121 gtctggctca ccgactgggg ggcgaacgcc ctcgtgcggt ttgatccgag gagagagacg
     3181 ttcgaagcct tcccgttccc gaccgccggg gcaagcgtcc gtcagatcct gggccggccg
     3241 ggcgaggtgt ggggcgccga gtccagcgtc gacagactgg tggtgtggcg gcggcgatga
     3301 gggggacgcg gggacctggg cgccggctcc atcccgggat gcttgcgata cgcgccgagc
     3361 tggtgcgttt cctggacgac accgccggcc ggtggaactg ggcccacaag ggcctgcgcg
     3421 aggcgatcaa agacctggat ccggatgagg cggtgtggaa gccgccgggg ggccactcgg
     3481 tgggggagca gatccgccac gtcgcgtact ggaagcgcta catcctgcac cgcctccagg
     3541 ggaagcggcc ccgcatgcgc caggcgtggc cggcgccggg acggacggcc gccgcgctcc
     3601 gccgggcgaa ggccgatctg ggggcgctgc accgggagct gcgcacgacg gtgatcggcc
     3661 tcgatcccga tgcgctcttc gagcgaaggg gccagagggg taccatcgcc cggctgctca
     3721 tgggcggagc cgcccacgag tcctaccata tcggccagat cctcctgacc cgaaagctgt
     3781 accggagacg tcaccgggtg cgctgagggc gtaacgagcc tgtgctcggc gccccatcgc
     3841 tggccgcttc gatcgagacg ctcctcggtc cccgcgcggt gttgcgggcc gcgggcacga
     3901 ccgccgcacc cacgaccgtg tagcgcggct cgaagaaccg ccggaccact tcgatgttgc
     3961 cgtaggagtc cggcagcggt tcctcgccgt cctcgatctc gaacgcggtg aagcgggcgg
     4021 gggccccgat cgcggcccgc ccccagtcct ccaaccccag gaatcgtgcg ggcgccaccg
     4081 tggcccgttc cacggcgtcc tccaccgaca cgccgagcat caccatcttg ctcatcgtcg
     4141 tggcgagatc ccagaccggc ccgtgcacgt tgccgatgtg aaggtcggtg ctgatggtgg
     4201 tcgggaggag gccgcgctcg accgcccggc gggccgtggg gtagtggaag ctgccctggc
     4261 cgtgcccgac gtcgagcacg agccccgccg ccgccatcgt ttcgcaccgg cggaaatagg
     4321 gctcgtggcg catgatcgag gtggtgatcc tgccggtaaa gcagtgggtg atcatgtcgc
     4381 ccggctcgag gatgtcgagg atctcgtcga gcgtgggtgg ggcttcgccg atgtgcacca
     4441 cgagcgggag ccgcaccgcc cgcgcgagcg ccttggccac ccggaccggg gtcatgcccc
     4501 actcgcggac gacctgattg ctcgcccgca ccttgatccc cttgatcagg tcgcggtgcg
     4561 cctcgacgac ctgggcggtg cgctccgggt ccagcaccgg gtccccgatg agttcgctga
     4621 cgcggttggc ggcgacgagg ccgatggtgc tgatgttgag gaatgccagg atcggaaacg
     4681 gcgcgggcgc gatgatgaac tcgcggaggc ccgcgaagtt ggcttccccg gcgctgccgc
     4741 agtccaccgc aagaccgacc ccggtcgagg gaccaacctg ctcgggccgc acggagagcc
     4801 acgttccccc atagtagcag tggacgtgca ggtcgatcca ggcgggacag agccgggcgc
     4861 ctcggaggtc gatgacctca tcgaaccgcg actggccctg ccagcggccc gcgagagcga
     4921 cccggccgtc tgccccgatc ccgatatcca ccgcagtgtt gttgggcacc cctccgagat
     4981 gcagggcctg acctccgcgg agcagcaccg agcccatacc gaccctcccg tgtggtggtg
     5041 acgttccccg cccgcggctc gtgccgtgtc aggagtgccg caggcgcgga tcgagaaaat
     5101 cccgcagccc gtcgccgagc agattgaggg cgagcacgct caagatgatc gcgaccccgg
     5161 ggaagatgct gatccacggc gcctgccgga ggtagacctg cccggcgctg atgatcccgc
     5221 cccaggtggg gatctgcggc gggacgccga ccccgaggaa actcagggcg gcctcgccca
     5281 gcatcgcgta cgcggtgatg aacgtggcct gcacgatgac cggggaggcg cagttgacca
     5341 ggatgtgcct ggccaggatc cgcgcgctcg acgcccccac cgcccgggcc gcttcgacgt
     5401 actccagccg gatgaccacg agcaccgcgg cgcggacgac ccgcgcgatc cgcggggtgt
     5461 agacgaagcc gagggacagg acgacgttgc gggcgctcgg cccgagcgcg gccatcaggc
     5521 cgatcgcgag caggacgtcc gggaacgcca tgaggccgtc catgatgcgc atcaggatcc
     5581 ggtccgcccg cgcgctcgag cccgccgcga gccccgcaag cacccccgcg gtggtcgcga
     5641 tggaggtcac gagcgccccg acgaggagcg acagccgggc gccgtagagg gtccggctca
     5701 ggatgtcccg cccgaactcg tccgtgccga aggggtgcgc gtcccgcggc ccctgcaggc
     5761 gatccgggac gttgagcgcg accgggtcgt acggggacag gagcggggcg gccgccgcgc
     5821 cgagcacgac gagcaagatc agcgccgcgc ccaccccggt gatccggcgc cggcggatca
     5881 tgcgccacag gagcgcggcc gcgccggggc gcgcgccgga aacgaggggc gaggttaggg
     5941 tgccctgggc catcggccgg tcagtacttc acccgcgggt ccacgaggaa gtagaggagg
     6001 tcgaccgcga ggttgatcag gacgtagatc gtcgcgacca gcaggatcac cccctggatc
     6061 acggggtaat cccgccgcag gacggacgag acgaccagct gcccgacccc ggggaggctg
     6121 aagaccgtct cggtgaccac cgcgcccccc atcagggctg cgaaggtgag ccccacgacg
     6181 gtcaggatcg ggaccagcgc gttgcgaaac gcgtggtgaa agatgacgac gcgctcgttg
     6241 acccctttcg cccgcgcggt ccggatgtag tcgttgccga tgacgtcgag gaggctcgag
     6301 cgggtgaacc ggatgatcag cgaggagttg ggcaggccca gggccagggc gggcaggagc
     6361 aggtagtgca ggctgagccc gacaccttca gtgggcgagc gataccccga cgacggcagc
     6421 caccccaggt tcacggcgaa gacgacgatg agggagaggc cgagccagaa gctcggcacc
     6481 gccgccccca gcatcgcgag cgcgaggctg ccctggtcca gccaggagtt gcggaacacg
     6541 gcagagacga cgccggcggg gatgccgatc agcaaggcga cggcgatcgc cagcaaggac
     6601 agcatcacgg tggggctggc gtgctgcagg atgtcctggg tcaccgggat gtgctggaag
     6661 agcgagagcc cgaggtcccc gcggatcgcc cggccgaacc agaagccaaa ctgcacgacg
     6721 agcggccgat cgagccccag gtcgtggcga agggcctgga cctgctccgg ggtcgcgtcc
     6781 tgcccgagga ggacccgcgc cggatcgccc ggggtcaggt ggatcagcag gaacacgacg
     6841 gccgcgacga ccagcagcac cggcacgagc gccaggagcc ggccgatcag gtacgagagc
     6901 acctacggat gcgcagcgcc cgcgaattcc cccttacttt gcgggctgga cgttccagag
     6961 caccaggaag tatgaggcgt ggaacccctg gacccccttg gcccacatct gcagggtgta
     7021 gtactccccc gggcggacga gcggggcctg ctgatagacg agttgctgca gtttggacca
     7081 gatgccggcg cgcgccccct ggtcgctcgt ccggttgaac tcatcgaaga gcttgttttt
     7141 ctccggcgag tcccaccatc ctgggtaccc cgggttgaag acggtgatca acgacgggtc
     7201 cgggacgaac ccgtgggacg tcgtgaacag gtcgtagtcg gccggcttgc cccggtggtc
     7261 gaggacgccc gcccactcga ggacctgcat gtccaccttg aacccgacgt gctgcagttg
     7321 ggcgaaggcg accaccgtgc tcttgaacat gtagtcgtac tgctgtgttg tcagccagcg
     7381 gacgggctcg ccgttgtagc cggcctcctt catgagct
//
LOCUS       DASQGH010000347         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52444, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000347 DASQGH010000000
VERSION     DASQGH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52444"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VEW91_06805"
     CDS             <1..918
                     /locus_tag="VEW91_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91327.1"
                     /translation="GVCAYTSVSVVGPDLTTIKSDIGSNGTIDTSQMSQAGTYVVAGV
                     SGKFTGKAVASSTITCNGACSGQIQGGAFPSSSGTICSALTVPTFTPGATALNITSGY
                     TMNSGTGYSWGNVTMPDSGSSGSCGNSYNTLSIQAGASGTNTVVQFNTLTMGYCTRLA
                     VLGAGNIELRIGKATLNGITTGGYGSSPNDVHFGVTSADTGSVLAPLAAGQLTVWVNS
                     NCTPPGCACAQNPPQGQTSCAAQISHIAGSAIFNVPNGSLNYDDSCPAYGTATAKTIL
                     FSGCAQLDASGFMSSPYTNFYNLRSWKDE"
     gene            complement(943..>1593)
                     /locus_tag="VEW91_06810"
     CDS             complement(943..>1593)
                     /locus_tag="VEW91_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM91328.1"
                     /translation="APELEGAEAVVRWLVSERITVAVGHTDAGYDEIITAAAWGARMV
                     THVFNAMRGFHHREPGSAGAALLAPVLTVGVIADLVHLHPASLQVIARVAGMGRIALV
                     TDAISAAGMGRGNFSLGGQTVEVRDGVPRLADGTFAGSVLAMVRGVYNFARAASVSLR
                     EAVQAATLVPARLLGLTHKGRITPGADADLVVLDREGSVALTLVGGEVAFRRSAPP"
BASE COUNT          272 a          554 c          525 g          242 t
ORIGIN      
        1 ggggtctgcg cctacacctc tgtgtctgtc gtgggcccgg atctcacgac catcaagagt
       61 gacatcggct cgaacggcac gatcgacacg tctcaaatga gccaggcagg gacgtacgtt
      121 gttgccggcg tttcgggcaa gttcactggg aaggccgtgg cgtcgagcac catcacctgt
      181 aacggcgctt gcagcgggca gatccagggc ggcgccttcc cgagcagctc cggcacgatc
      241 tgcagtgccc tgacggtccc caccttcacc ccgggggcca ccgccttgaa catcacgtcg
      301 ggctacacga tgaacagcgg caccgggtat agctggggca acgtcaccat gccggatagc
      361 gggtcgtcgg gctcctgcgg caactcgtat aatacgctca gcattcaggc cggcgcgtcg
      421 ggcacaaata ccgtggtgca attcaacacg ctcaccatgg ggtattgcac gcgcctggcc
      481 gtcctcggcg cgggaaatat cgagctgcgc atcggcaagg cgaccctcaa cgggatcaca
      541 acgggggggt acggatcctc cccgaacgat gtccatttcg gtgtgacctc cgccgatacg
      601 gggagcgtgc tcgcgccgct cgcggcgggc cagctgaccg tgtgggtgaa ctcgaactgc
      661 acgccgccgg ggtgcgcgtg cgcccaaaac ccaccccagg gacaaacatc gtgcgcggcg
      721 cagatcagcc acattgccgg ctcagccatc ttcaatgtgc cgaacggctc gttgaactac
      781 gatgacagct gtcccgccta tgggaccgcc acggccaaga ccatattgtt cagcgggtgc
      841 gcccagcttg acgcgtcggg cttcatgtcg tctccctata ccaacttcta caacctgcgg
      901 tcttggaagg acgagtgaag ggggggcggg gcgcgcggcc ggctatggcg gcgcgctgcg
      961 gcggaacgcg acctcgcctc ctaccagggt cagagccacg ctcccttccc ggtcgagcac
     1021 gacgaggtcg gcgtcggccc cgggcgtgat ccgtcccttg tgcgtcagcc ccaatagccg
     1081 ggcgggaaca agggtggccg cctgcacggc ctcccgcagg ctcaccgacg ccgcgcgcgc
     1141 aaagttgtag accccccgga ccatcgcgag cacgctgccg gcgaacgtgc cgtctgccag
     1201 acgcgggacg ccgtcccgga cctccaccgt ctgccccccc agggaaaagt tcccgcgtcc
     1261 catgccggcg gcggagatcg cgtccgtgac gagcgcgata cggcccatcc ccgccacgcg
     1321 cgcgatgact tgcagcgagg ccgggtgcag gtggacgagg tccgcgatga cccccacggt
     1381 gaggaccgga gcgagcagcg ccgcgccggc ggagcccggc tcgcggtggt ggaatccccg
     1441 catcgcgttg aacacgtggg tgaccatgcg ggcgccccag gcggctgcgg tgatgatctc
     1501 gtcgtacccg gcatcggtgt gaccgaccgc caccgtgatc cgctctgaga ccagccaccg
     1561 gacgacggcc tcggccccct cgagttcggg cgc
//
LOCUS       DASQGH010000348         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_12944, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000348 DASQGH010000000
VERSION     DASQGH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12944"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VEW91_06815"
     CDS             <1..903
                     /locus_tag="VEW91_06815"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HYM91329.1"
                     /translation="ETGQAIRDAIGAGERWGFRVTYILQDAPAGLAHAVKAARSFLGS
                     SPFLMFLGDNLIQGGVQPLIQRFERSQAEALILLKEVPDPRQFGVAVLNGDGSVRTLV
                     EKPQDPPSRLALVGLYLFRPAVHAAIDRIRPSQRGELEITDAIQELLREGRRVETVRL
                     EGWWLDAGKKDDLLEANRTVLDEFTVRNVLGSVDPASSVDGRVEIGRATVVEGSVIRG
                     PVVIGERCRIQGAFIGPYTAIGSDTVVSRTSIQHSVVLDHCHLEGIDRLEDSVLGRAV
                     TVRRSADGPKALRLFVSDDSQITI"
     gene            907..>1646
                     /locus_tag="VEW91_06820"
     CDS             907..>1646
                     /locus_tag="VEW91_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HYM91330.1"
                     /translation="MASLSVIVPTYNEAANVGPLIARIARALAAVDYDLIFVDDSTDG
                     TADVLAELARGAPRLRVVHRQDRRGLASAVTEGIALARGDVVCVLDADLQHPPEILPA
                     LMEALERTGADLAIASRYVPGGSNTTLSLSRRLVSRGAIALARLLLARARRVADPVSG
                     FFAFRHRIVEGVPLRPIGYKILLEVLVRGNVTSVVEVPYRFDTREAGESKLTLVQNWF
                     YLKHVLALATVHPADAEPIRLSVIALTAL"
BASE COUNT          238 a          589 c          572 g          247 t
ORIGIN      
        1 gagaccgggc aggcgatccg ggatgcgatc ggagcgggcg agcggtgggg attcagggtc
       61 acgtacatcc tccaagacgc ccccgccggg ctggcccacg cggtcaaggc ggcgcgctcg
      121 ttcctgggca gtagcccgtt cttgatgttc ctcggggaca acctgatcca gggcggcgtc
      181 cagcccctca tccagcggtt cgagcgcagc caggcggagg cgctgatcct gctgaaggag
      241 gtcccagacc cgcgccagtt cggggtggcc gtcctcaacg gcgatggaag cgtgcgcacc
      301 ctggtcgaga aaccccagga tcctccgagc cgcctcgcgc tcgtcgggct gtacctgttc
      361 cgccccgcag tacacgccgc gatcgaccgc atccgcccct cccagcgcgg cgaactcgag
      421 atcaccgacg cgatccagga gctcctccgc gagggaaggc gcgtcgagac ggtgcggctc
      481 gaggggtggt ggctggacgc cgggaagaag gacgatctgc tcgaggccaa ccgcaccgtg
      541 ctggacgagt tcacggtgcg caacgtcctc gggagcgtcg atcccgcctc ctccgtcgac
      601 ggccgggtcg agatcggtcg ggcgacggtg gtggaaggga gcgtgatccg cggaccggtc
      661 gtgatcggcg agcgctgccg gattcagggc gcgttcatcg gcccctacac cgcgatcgga
      721 agcgacaccg tcgtctcccg gaccagcatc cagcactcgg tcgtcctcga ccactgccac
      781 ctcgagggga tcgaccgcct cgaggacagc gtcctggggc gcgcggtcac ggtgcggcgg
      841 tccgcagacg gcccgaaggc gctcaggctc ttcgtcagcg acgactcgca gatcacgatc
      901 tagctgatgg cgtccctgtc ggtcatcgtc ccgacctaca acgaggccgc gaatgtcggc
      961 ccgctgattg cgcggatcgc gcgggcgctc gccgcggtcg actacgacct gatcttcgtc
     1021 gatgacagca cggacggcac ggccgacgtc ctcgccgagc tggcccgggg ggcgccgcgc
     1081 ctccgggtgg tgcatcgcca ggaccgccgg gggctggcgt cggcggtcac cgaggggatc
     1141 gcgctcgccc gaggcgacgt cgtgtgcgtg ctcgacgccg acctccagca tcccccggag
     1201 atcctcccgg cgctgatgga ggccttggag cggaccggcg ccgacctggc gatcgccagc
     1261 cgctacgtcc ccggcggcag caacacgacc ctctccctgt cccgccgcct ggtctcccgc
     1321 ggggccatcg cgctggcccg gctcttgctg gcgcgggccc gccgggtggc ggatccggtg
     1381 tcaggattct tcgcgttccg ccacaggatc gtcgagggcg tccccctccg gccgattggg
     1441 tacaagatcc tgctggaggt cctcgtccgg gggaacgtca cgagcgtcgt cgaggtgccg
     1501 taccgcttcg atactcgcga ggccggggag agcaagctca cgctcgtgca gaactggttc
     1561 tacctcaagc acgtccttgc gctggccacc gtccaccccg cggacgccga accgatccgg
     1621 ctcagcgtga tcgccctcac cgcgct
//
LOCUS       DASQGH010000349         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41130, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000349 DASQGH010000000
VERSION     DASQGH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41130"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..1150)
                     /gene="pheA"
                     /locus_tag="VEW91_06825"
     CDS             complement(326..1150)
                     /gene="pheA"
                     /locus_tag="VEW91_06825"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HYM91331.1"
                     /translation="MRVAFQGERGAYSEEAVVRLFGEVEVVPCAYLGDAFEAVASKRA
                     DRGVVPVENSQAGSINETYDLLLAHDLVIAGELDHRVVHCLLALPGQSLEAIKRVYSH
                     PQALAQCGAYLRRIGAETVPSYDTAGSAKMIQEQRLLGCAAIAARRAARLYDLEVLAE
                     GIETNPNNFTKFLVIAPEPAPRSAAGKTSIVFAVENRPGTLYQALGALATRQINLVKI
                     ESRPGRTRPWDYIFYVDIDGHASDTRIREALEDLCGRTTFLRVLGSYPRSPAPPAP"
     gene            complement(1163..>2120)
                     /gene="alr"
                     /locus_tag="VEW91_06830"
     CDS             complement(1163..>2120)
                     /gene="alr"
                     /locus_tag="VEW91_06830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYM91332.1"
                     /translation="TDEGVQLRRAGIESPILLLGHTPPEDAETAVAHALSVTVFQVDA
                     ARALSRAASGVGRPARVHVKIDTGMGRIGVAPVDAASVVREVRALGGIVLEGCFTHLA
                     TADEPNAEPARAQLDAFRRALRPLEEEGIPLGLRHAANSAAMLALPESHLDLVRPGIA
                     VYGISPAPHLGGRITLRRVMRFRARVTHAKRVPPGTPIGYGRTYRADRATTIATIPVG
                     YADGYPRLLSGRGEVALGGRRLPIAGRVSMDQCTFDAGDAAVRVGDEVELWGDAVPVE
                     EVAERAQTISYEVLSGLSRRVPRVFVQDGQVIGVRTLLSSGG"
BASE COUNT          324 a          768 c          705 g          323 t
ORIGIN      
        1 ctcgattccg ctctcccgaa tgcggacctg cgattaccgc tcgctctacc gcgtcgatga
       61 gacactcgtc acgatcatgc cgatcatcaa tcgccgcgcc cttgaccgta cacttcgaca
      121 actccgtctc tgaactaaag cgttcttcca gcgcagcagg gatagcgtag aaacgtgcag
      181 acagcgtgcg cggcgctggc aacttttcga gacgcgccgg acggaggaat ggtccaaagt
      241 tatgcttctg gtgtccgtaa cgtcctacta agatgattcg acgatatcct cttcggtatg
      301 attcatagat gatttgacgg gaacgtcacg gggcaggagg cgccggcgag cgcgggtacg
      361 agccgagcac ccggaggaac gtggtacgcc cgcacaggtc ctcgagcgcc tctctgatcc
      421 gggtgtcgct ggcgtggccg tcgatatcca cgtagaagat gtaatcccac ggccgcgtcc
      481 gcccggggcg cgactcgatc ttcaccaggt tgatctgccg ggtcgccagc gcgccgagcg
      541 cctggtacag cgtcccgggc cggttctcga ccgcgaacac gatcgacgtc ttgcccgccg
      601 ccgaccgggg ggcgggctcg ggggcaatga ccaggaactt cgtgaagttg ttcgggttgg
      661 tctcgatccc ctccgccagg acctcgagat cgtagagccg cgccgcccgg cgcgcggcga
      721 tggcggcgca ccccaggagg cgctgctcct ggatcatctt cgcgctcccc gccgtgtcgt
      781 aggacgggac cgtctcggcg ccgatccgcc ggaggtacgc tccgcactgg gccagcgcct
      841 gcgggtgcga atagacccgc ttgatcgcct ccagggactg tccgggaagc gccagcaggc
      901 agtgcaccac gcggtggtcg agttcgccgg cgatcacgag gtcgtgggcc agcaacaggt
      961 cgtacgtctc gttgatgctg cccgcctggg agttttccac cggcacgacc ccgcggtccg
     1021 cccgcttcga tgcgaccgcc tcgaacgcat cgccgaggta ggcgcacggg accacctcca
     1081 cctccccgaa gagacgcacc accgcctcct cgctgtaggc gccccgctcc ccctgaaacg
     1141 ccacgcgcac gggcagctcc cctcacccgc cgctgctgag caaggtccgg accccgatca
     1201 cctggccgtc ctggacgaac accctcggca cgcgccggct cagtccgctc aagacctcgt
     1261 acgagatcgt ttgcgcccgc tcggcgacct cctccacggg caccgcatcg ccccacagtt
     1321 cgacctcatc ccccacccgg accgccgcat cccccgcgtc gaacgtgcac tggtccatcg
     1381 agacccgccc ggcgatcggg aggcgccggc cgccgagtgc gacctcgccg cgcccgctga
     1441 ggaggcgcgg gtagccgtct gcgtatccca ccgggatcgt ggcgatcgtg gtggcgcgat
     1501 cggcgcggta ggtccgcccg tacccgatcg gcgtgccggg cgggacccgc ttcgcgtggg
     1561 tcacccgcgc gcggaagcgc atcacccggc ggagcgtgat ccggcccccc aggtgcgggg
     1621 cgggagaaat cccatacacc gcgatcccgg gccggacgag atcgaggtgc gactcgggga
     1681 gcgccagcat ggccgcgctg ttggccgcat ggcggagccc gagggggatg ccctcctcct
     1741 ccagtgggcg gagggcgcgc cggaatgcgt cgagctgggc cctggccggc tccgcattgg
     1801 gctcgtcggc cgtggccagg tgcgtgaagc acccttccag gacaatcccg ccgagggcgc
     1861 gcacctcgcg caccacggac gccgcatcca ccggggcgac gccgatgcgt cccatccccg
     1921 tgtcgatctt gacgtgcacc ctcgccgggc ggcccacgcc ggaggcggcc cggctcagcg
     1981 cccgggcggc gtccacctgg aagacggtca ccgagagcgc gtgcgccaca gcggtttcgg
     2041 cgtcctctgg aggggtgtgg ccgaggagca ggatcggcga ttcgatgccg gcgcggcgca
     2101 actgcacgcc ttcgtcggtg
//
LOCUS       DASQGH010000350         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_9982, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000350 DASQGH010000000
VERSION     DASQGH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9982"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VEW91_06835"
     CDS             <1..664
                     /locus_tag="VEW91_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYM91333.1"
                     /translation="AQVLAEVRRHTAAQILVINDGSTDGSARILGRSLPGIGVITHAQ
                     NEGYGQSLIDGFTFAIREGFDVAVTIDCDLQHEPRLVPRFLEAAHAFDIVSGSRYHPD
                     APGAQDPAPPDRQRINAEVTRIINDLTGYRLTDAWCGFKAYRVQGLARMTLDERSYGL
                     PLQVWLQAARLGLTVTEIPVPRIYKNPDRRFWGGLDDPEARRTYYLSIIDREASRWRN
                     SS"
     gene            646..1329
                     /gene="bshB1"
                     /locus_tag="VEW91_06840"
     CDS             646..1329
                     /gene="bshB1"
                     /locus_tag="VEW91_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HYM91334.1"
                     /translation="MAELLVIGAHPDDVEIGMGGAIATFVQQGHAVAILDLTDGEPTP
                     MGSPERRRAESEAAARALGVQRRITLPLPNRYLMDTVENRIAVAEAIREVRPEIVFLP
                     YGVDAHPDHVAAERLGEAARFYAKLTKTEMRGDPHYPRRILHFFCTHYRLHVAPAFIL
                     DITDQIERKMEAIACYRSQFNEERGNTGILDAIRTVAAYWGTRIRRPYGEPFASKEVV
                     GLRSLGDLL"
     gene            1326..>2195
                     /locus_tag="VEW91_06845"
     CDS             1326..>2195
                     /locus_tag="VEW91_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91335.1"
                     /translation="MIQRSLAPMTYKVPERSGEVLITPPLDRIPALLAAARERSWGPT
                     HILGTPLEEVRRRIRQRALAQAGPQPPDASPELRAPLVLMGHQPLFFHPGVWIKFFLL
                     TRLHRALGVTALHLNVDSDAAGPISCPIPMRTDQVTRRTETLVDLPDDLPLESAAPPA
                     RDMWEAFCTRVRGHLATLGSAELPARFDAFVQGAAQSLGRARTLGGFLAGARRSYEAR
                     STPPGYLELPVSALSDTPEFRAFALHLIQDPAGFRGRYNASLEEYRSTHRVRSAANPF
                     PNLGEGEGFCETPL"
BASE COUNT          330 a          805 c          725 g          335 t
ORIGIN      
        1 cgcccaggtg ctggcggagg tccgccgtca cacggccgcc cagatcctcg tcataaacga
       61 cggatccacc gacgggtcgg cccgcatcct cgggcgcagc ctgcccggca tcggggtgat
      121 cacccacgcg cagaacgaag ggtacgggca gtcgctgatc gacgggttta cgttcgcgat
      181 ccgggaagga ttcgacgtcg cggtgaccat cgactgcgac ctccagcacg agccgcggct
      241 ggtcccccga ttcctggagg ccgcccatgc gtttgacatc gtctcgggga gccgctacca
      301 tcctgatgcg ccgggggccc aggatccggc gccgcccgac cggcagcgga tcaacgcgga
      361 ggtgacccgg atcatcaacg atctgaccgg ctaccgcctg acggatgcgt ggtgcgggtt
      421 caaggcgtac cgggtccaag gcctggcccg gatgaccctc gacgaacgca gctacgggct
      481 tccgcttcaa gtgtggctgc aggccgcgag gctcgggctc accgtcaccg agatccccgt
      541 cccccggatc tacaagaacc ccgaccgtcg gttttgggga gggctcgacg acccggaggc
      601 gcgccgcacc tactacctct cgatcatcga ccgcgaggcg agccggtggc ggaactcctc
      661 gtgatcggcg cccatcccga tgacgtggag atcgggatgg gcggggcgat cgccaccttc
      721 gtccaacagg ggcacgcggt tgcgatcctc gatttgaccg atggcgagcc gaccccgatg
      781 gggtcgccgg agcggcggcg cgcggagagc gaagccgcgg cccgcgccct cggcgtccag
      841 cgccggatca ccctgccgct tcccaaccgc tatctgatgg ataccgtcga gaaccggatc
      901 gccgtcgccg aagccatccg ggaggtccgt cccgagatcg tgttccttcc ctacggggtg
      961 gatgcgcatc ccgatcacgt ggccgcggag cggctcgggg aggcggcgcg gttctacgcc
     1021 aagctcacga agaccgagat gcggggcgat ccccactatc cgcgccggat cctgcacttc
     1081 ttctgcacgc actaccggct ccacgtcgcc cccgccttca tcctggacat caccgatcag
     1141 atcgagcgga aaatggaagc cattgcctgc taccgttcgc agttcaacga ggagcgcggc
     1201 aacaccggga tcctggacgc gatccggacc gtcgccgcct actgggggac gcgcatccgc
     1261 cggccgtatg gggaaccgtt cgccagcaaa gaggtggtcg gcctccgcag cctcggggac
     1321 ctgttgtgat ccagcggtct ctcgccccga tgacctacaa ggtgccggag cgttcgggcg
     1381 aggtgctgat caccccgccg ctcgatcgca tcccggcgct tctggccgcc gcccgggagc
     1441 ggtcgtgggg gcccacccac atccttggaa ccccgctcga ggaggtccgc cgcaggatcc
     1501 ggcagcgcgc cctggcccag gccggccccc agccgccgga cgcctccccg gagctccgag
     1561 ccccactcgt cctcatgggc caccagccgc tgttcttcca tccgggggtg tggatcaagt
     1621 tctttctcct gacccgcctg catcgggcgc tgggggtcac cgccctccac ctgaacgtcg
     1681 acagcgacgc ggcggggccg atctcctgcc ccatcccgat gcggacggac caggtgaccc
     1741 ggcgcaccga aacgttggtc gatctcccag atgatctccc gctcgaatcc gccgcccccc
     1801 cggcccggga catgtgggaa gccttctgca cgcgcgtgcg ggggcatctg gcgaccctcg
     1861 ggtcggcgga gctcccggcg cgcttcgacg ccttcgtcca gggggccgcc caatcactcg
     1921 ggcgcgcgcg gacgctcggc gggtttctcg ccggcgcccg ccggagctac gaggcccggt
     1981 cgaccccgcc gggctatctc gagttgcccg tctccgcgct ctcggacacc cccgagtttc
     2041 gggcgttcgc gctgcacctg atccaggatc ccgcgggatt ccgggggcgg tacaacgcga
     2101 gtctggagga gtaccggtcg acccaccggg tccgatcggc ggccaacccg tttccgaacc
     2161 tcggcgaagg cgagggcttc tgtgagacgc cgctc
//
LOCUS       DASQGH010000351         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41145, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000351 DASQGH010000000
VERSION     DASQGH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41145"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VEW91_06850"
     CDS             complement(<1..487)
                     /locus_tag="VEW91_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HYM91336.1"
                     /translation="MEFKDYYKVLGVDRKADQKAVSQAFRRLARQHHPDVNPGNKQAE
                     TRFKEINEAYQVLGDPERRAKYDQVLELRARGGGWEDLLRRGAERGADGTYTVYGSPE
                     DLAQFSDFFQQLFAGLGGGTFSEAFGGGARPRRRGGVRIEDLFEQRAGGRGGAEDLEG
                     AV"
     gene            complement(500..1096)
                     /locus_tag="VEW91_06855"
     CDS             complement(500..1096)
                     /locus_tag="VEW91_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYM91337.1"
                     /translation="MEQRDERPEVEGAPEGRPGEESVAPADDLAGLSETELRAKLQEA
                     REDVQRNWQHFLHSAADLENYKKQAARDRQDAVERTRRQMLTLVLSVVDNLERALAAV
                     SGQPSADPAVIEGLRITHRQILGQLEVFGVRTIEAVGKAFDPRLHEAVGVVPPQEAGP
                     GIGMVVGETLRGYLLNDEVLRPAKVIVVGEAAEEKAKR"
     gene            complement(1130..>1744)
                     /locus_tag="VEW91_06860"
     CDS             complement(1130..>1744)
                     /locus_tag="VEW91_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYM91338.1"
                     /translation="QGERPMARDNRTLGKFLLDGLPPAPRGIPQIDVTFDIDANGILN
                     VSAKDKATGREQSIKITGTGTLDKKEVDRLVKEAESHAQEDQRQRDAAEIKNRGDSLA
                     YQTERMLKEVGDKVPAEDRGKVESALGELKEALKAGDSDRIKRASEALQQASSKLGEE
                     MYKRATAGAAGAGAGAGTAGGQPGGSGKGNEDVIDAEFKPSDKQ"
BASE COUNT          208 a          662 c          537 g          337 t
ORIGIN      
        1 cgaccgcgcc ctccaggtcc tcggcacccc cgcgcccgcc ggcgcgctgc tcgaagaggt
       61 cctcgatgcg gacccctccg cgccgtcggg gccgtgcgcc tccgccaaac gcttcggaaa
      121 acgtcccgcc gccgagccca gcaaagagct gctggaagaa atcgctgaac tgcgcgaggt
      181 cttcgggcga gccgtacacc gtatatgtgc catcggctcc ccgctccgcc ccgcggcgca
      241 ggaggtcctc ccacccgccg ccgcgcgccc gcagctcgag cacctggtcg tacttggccc
      301 gccgctccgg atcgccgagc acctggtagg cttcattgat ctctttgaag cgggtctcgg
      361 cctgcttgtt gccggggttc acgtcgggat ggtgctggcg tgccagcctg cggaaagcct
      421 ggctgacggc cttctgatcg gccttgcggt caaccccgag gaccttgtag tagtccttga
      481 actccatcgc ggcgcgcccc taccgcttgg ccttctcctc ggcggcttcg ccgacgacga
      541 tgactttggc gggtcgaagg acctcgtcgt tgaggaggta cccccggagg gtctcgccga
      601 cgaccatccc gatccccggc cccgcctcct ggggcggcac cacccccacc gcctcgtgca
      661 gccggggatc gaacgccttc ccgaccgcct cgatggtccg cacgccgaat acctcgagct
      721 gccccaggat ctggcggtgg gttatccgca ggccttcgat gaccgccggg tccgcgctgg
      781 gctggccgga cacagcggcc agcgcccgct caagattgtc gaccacgctg agcacaaggg
      841 tcagcatctg ccggcgggtg cgctcgacgg catcctgccg gtcccgggcc gcctgcttct
      901 tgtagttctc cagatccgcg gcggaatgca ggaagtgctg ccaattccgc tggacgtcct
      961 cccgggcctc ctgcagcttc gccctcaact cggtctctga aagaccggcg aggtcgtcgg
     1021 ccggggcgac cgattcttcg cccggccgcc cttccggtgc gccctcaacc tcgggacgct
     1081 cgtcgcgctg ctccatcggt cgtcacactc cggctttgat gcgtcccgcc tattgcttgt
     1141 ccgacggctt gaactcggcg tcgatgacgt cttcgttgcc cttgccgctt cccccgggct
     1201 gtcccccggc cgtcccggcc cccgcgcccg ctccggctgc cccggctgtg gctcgcttgt
     1261 acatctcctc gcccagcttg gaactggcct gctgcagcgc ctcggaggcg cgcttgatcc
     1321 ggtcggagtc gccggctttc aacgcttctt tgagctctcc aagcgccgat tcgacctttc
     1381 cgcgatcctc ggccgggact ttgtccccga cctccttgag catccgctcg gtctgatacg
     1441 cgaggctgtc gccccggttc ttgatctccg cggcgtcccg ctggcgctgg tcctcctgcg
     1501 cgtgcgactc ggcctctttg accaggcggt cgacctcctt cttgtccagc gtgccggtcc
     1561 ccgtgatctt gatcgactgc tcgcgtccgg tggccttgtc cttcgccgag acgttgagga
     1621 tgccgttggc gtcgatatca aacgtcacat cgatctgcgg gatgccccgg ggcgccggcg
     1681 gaaggccgtc gagcaggaac ttgccgagcg tccggttgtc ccgggccatc ggccgctcgc
     1741 cctg
//
LOCUS       DASQGH010000352         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49649, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000352 DASQGH010000000
VERSION     DASQGH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49649"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VEW91_06865"
     CDS             complement(<1..367)
                     /locus_tag="VEW91_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91339.1"
                     /translation="MSAARRRGGLLHGIFLVLIVAYSLFPIYMMAIESLKSVDEDVFG
                     NPLLLSHPDTQWYEDLFEPAEWVRGTASVDVIPFLVWLKNTGIVFGATLVIVLSTGIA
                     AGYALGRLRPPGWRWWRRAL"
     gene            complement(364..1305)
                     /locus_tag="VEW91_06870"
     CDS             complement(364..1305)
                     /locus_tag="VEW91_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM91340.1"
                     /translation="MSQAVAAAPETAVGGSPGSGAAPVRRRREVLAAYLLLAPAVVLI
                     LGPLAYPIAWEGWVSVFNFSTQQTRPTFIGLHNYGELLQDSAFWWATANTAGYIVLTS
                     ILKLAIGVGVALALARPFPGRPFVFLAAFLPWAYPASVALIGWYWFIIPPVHAAYSPF
                     LAALQFTIDRWLGDGTWGFLSVVALNTWRGGSFTGIFLLAALNGIPQDVFDYAALEVR
                     SGWRRFWMVTVPLLRPFLALATFLSFATAVTDLGNVWFQSGQRIVYPIVWTQAMQDAL
                     IGGQWGKASALSLILLPVLIVFLLACFRLFEPLEEST"
BASE COUNT          323 a          521 c          570 g          235 t
ORIGIN      
        1 acagcgcacg ccgccaccag cgccatcccg ggggacgaag acgcccgagc gcatagccag
       61 ctgcgatccc cgtcgagagg acgatcacca gcgtcgctcc gaagacgatg ccggtgttct
      121 tgagccagac caggaaggga atcacatcca ccgaagcggt gccgcggacc cactcggcgg
      181 gctcaaacag gtcttcgtac cattgagtgt ctgggtggga caggaggagg gggttcccaa
      241 agacatcctc gtctacgctc ttcaaagact cgatggccat catatagatc gggaagaggc
      301 tgtaggcgac gatgagaacc aggaaaatgc cgtggagcaa ccccccacga cgccgcgcgg
      361 cgctcatgtg gactcctcca gcggctcgaa gagccggaag caggccagga ggaacacgat
      421 caggaccggc agcagaatga gggagagggc cgacgccttc ccccactggc ctccgatcag
      481 cgcgtcctgc atcgcctgcg tccacacgat cgggtagaca atgcgctggc ccgactggaa
      541 ccagacgttc ccgagatcgg tgacggcggt ggcaaaggag aggaacgtcg ccagggccag
      601 gaacggccgg aggagcggaa cggtgaccat ccaaaacctc cgccacccgc tccgcacctc
      661 cagcgcggca tagtcaaaga catcctgcgg gatcccgttg agggccgcga ggaggaagat
      721 cccggtgaac gatccccccc gccaggtgtt gagcgcgacc acgctgagga atccccaggt
      781 cccatcgccc agccagcgat cgatggtgaa ctggagcgcc gcgagaaacg ggctgtaggc
      841 ggcgtggacc gggggaatga tgaaccagta ccacccgatc aatgccacac tggcgggata
      901 ggcccacggg aggaatgcgg cgagaaacac gaacggccgg cccgggaatg gccgcgcgag
      961 ggccagggct accccaacgc cgatcgctag cttcaggatg cttgtgagga cgatgtatcc
     1021 ggcggtgttg gcggtggccc accagaatgc cgaatcttga aggagttcgc cgtagttgtg
     1081 gagcccgatg aaggtcgggc gcgtctgttg ggtagagaag ttgaagacgc tgacccatcc
     1141 ctcccaggcg atcgggtacg ccagcgggcc caggatgagc accaccgcgg gagcgaggag
     1201 caggtaggca gcgagaacct cgcggcgccg gcggaccgga gcggctccgc tcccggggga
     1261 cccccccacc gcggtctcgg gggccgccgc gacagcctgc gacatggggg agtcttccag
     1321 gggcgtccgg gggattcctt gggctgcacc cggccagtcc ggcaatccca ccgctcggcc
     1381 ggagggccac aacgccctga tcgtcgggtc cgtcggcggg cgcaccctcg ggaacgtgct
     1441 cgcgatgatc gcgcccgggg ggacgtgtgt gtccttaggc gcctcatcgg gtgctggggc
     1501 gatgttcaac agcggttcgt tctttacgac gggtggcgtg agtctctacg gcttgttcct
     1561 gctctacgag ttgcggcacg agtcagcggc tgtgggactc gtccggttac tgcggcttct
     1621 gatcgacggg cgcctgcgcc cccacatcg
//
LOCUS       DASQGH010000353         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1476, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000353 DASQGH010000000
VERSION     DASQGH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEW91_06875"
     CDS             complement(<1..191)
                     /locus_tag="VEW91_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91341.1"
                     /translation="MHTSLPASEDDLIQRLQGAHTAINIRGYCKFTARVLGAVGGTLR
                     HLAIWGTGTDNVDLPEAQKR"
     gene            409..1536
                     /locus_tag="VEW91_06880"
     CDS             409..1536
                     /locus_tag="VEW91_06880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYM91342.1"
                     /translation="METADVVIIGGGLIGCASAYYLAARGIRPTVLERRGLATEASGS
                     NAGMVGASGGIPGKTLAHTKKSLELLAKDAEELGRPVELVREGRVVLAAGEAEWAEAQ
                     EFAATRQREGVETHLLSSDDLRRLEPGLGPGFVGAAFVPGDGHVNPFLLTHAYAGAAQ
                     RHSAEVQLGVEAMRVEVSRGRVTGVLTQAGRIAAPRVVVAAGAWSRTLLAPLGIPLPV
                     RPGRGQMLVTEALPPLTPRVLRTPAIGIRQDVRGHMLIGSTVEDVGYNREVTLPTLGL
                     FSRLAIALLPALKDARIIRTWAGLRPMTPDSLAIIDTAPGVEGLYLATGHSRTGLTYG
                     PISAWLLAQLIAEGGTQLPLDPFRLGRFAQAEAGAPVPGRA"
     gene            1547..2695
                     /locus_tag="VEW91_06885"
     CDS             1547..2695
                     /locus_tag="VEW91_06885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM91343.1"
                     /translation="MRAARDRETMTRCVVIGAGVVGASVAHRLAESGASVTVLEAGRV
                     GGGTSGVSFAWTNSNNKPPRAYHDLNVAGMRAHAALAEEFGDTPWWHGGGCVEWARTE
                     ADRSGLRARVERLRSWGYAAEWLTLQQLRALEPDIDPAAVGEGPIAYFPEEGWIDPVV
                     YAHAMLEAAQRSGAALRCGARVTEIRVEGGRVTGVRTAAGESFGADVVVNCAGRWADE
                     VARLAGLRIPLAPTVGLLVFTPPVATCLRRVVRAPQCHMRPDGGGRLVLRTEDADRQV
                     SAETEPSPRLAPAEDLVRGAAQVLPGIGGTGPEAARIAVRPIPSDGYSAVGPMPGVSG
                     SYLVVTHSGVTLAPFLGRAVAEEVTRGRTVRELERFRPSRFAGPTGGG"
     gene            complement(2774..3088)
                     /locus_tag="VEW91_06890"
     CDS             complement(2774..3088)
                     /locus_tag="VEW91_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91344.1"
                     /translation="MIMVMGLLVAGLGGQIHPASAQMESSYDKATAALQAAQAAQAQA
                     QKQLATTMTHMDSMKKMQMSSMEKEMMGGMEQMAATMKMMLDTNRKLLDAIKDLIDAG
                     KK"
BASE COUNT          434 a         1169 c         1238 g          532 t
ORIGIN      
        1 cgcttctgcg cttcggggag gtcgacgtta tcggtgcccg tgccccagat cgccaggtgc
       61 cggagcgtcc cgccgaccgc ccccaggacc cgggcggtga acttgcagta gccgcggatg
      121 ttgatggcgg tgtgggcgcc ctgcaggcgc tggatgagat cgtcctcgga ggcaggcagg
      181 gaggtgtgca cggtcgtctc cccggccgcg cggatccgct caagggccgg ggtgccggag
      241 atgaccgacg ggctgtcgtc gggaatgacg atccggaccg tcgcgatccc ccccttctat
      301 acgatggggc catgcggcgc atgcccccgg gtcctaacag gttcgatagc ggcttcgatc
      361 tgcctccata ggaggattcg ccccgcggcc ggctgaagag cgaccctggt ggagacggct
      421 gatgtggtga tcattggcgg gggcctcatc ggctgcgcct ccgcgtacta tctggccgcc
      481 cgaggtatcc ggccgacggt cctcgagcgg cgcgggctcg cgacggaggc gtccggctcc
      541 aacgcgggga tggtgggggc ctcggggggg attcccggaa agacgctcgc gcacacgaag
      601 aagagcctcg agctccttgc gaaggacgcg gaagagctgg ggcgcccggt tgagctcgtc
      661 cgcgaggggc gggtcgtcct ggccgcgggc gaggcggagt gggcggaggc ccaggagttc
      721 gcggccacgc ggcaacggga gggcgtggag acccacctgc tctcctcgga cgaccttcgg
      781 cggctggagc ccgggctcgg ccccgggttt gtcggggccg cgttcgtgcc cggggacggg
      841 cacgtcaacc cctttctgct gacccacgcg tacgccggcg cggcgcagag gcacagcgcc
      901 gaggtccagc tcggggtgga ggcgatgcgc gtggaggtct cgcggggccg cgtcaccggc
      961 gtgctgaccc aggcgggccg gatcgccgcc ccgcgtgtcg tcgtcgccgc gggcgcctgg
     1021 tcgcgcacgc tgctcgcccc gctcgggatc cccctcccgg tgcgtcccgg gcgcggccag
     1081 atgttggtca ccgaggccct gccgccgctc acgccgcgcg tcctccgtac cccggcgatc
     1141 ggcatccggc aggatgtccg cggccatatg ctcatcggga gcacggtgga agacgtcggc
     1201 tacaaccggg aggtgacgct cccgacgctc ggcctgttct cccgcctcgc gatcgcgctg
     1261 ctgccggccc tcaaagatgc ccggatcatc cgcacctggg cggggctgcg gcccatgacc
     1321 cccgacagcc tcgcgatcat cgataccgcg ccgggggtgg aaggtctgta cctggccacc
     1381 gggcacagcc ggaccggcct gacctacgga ccgatctcgg cctggctgct cgcccagttg
     1441 atcgccgagg gcggcacgca gttgcccctc gacccgttcc ggctcgggcg gttcgcgcag
     1501 gcggaagccg gcgcccccgt ccccggccgc gcctgatcga tccatcatgc gggcggcccg
     1561 ggaccgggag accatgactc gctgtgttgt gatcggcgcc ggcgtcgtcg gggcgtcggt
     1621 ggcccaccgc ctcgccgaat ccggcgcctc ggtcaccgtc ctggaggccg gccgggtggg
     1681 cgggggaacg tcgggggtga gcttcgcctg gacgaactcc aacaacaagc cgccgcgggc
     1741 gtaccacgac ctgaacgtcg ccgggatgcg ggcgcacgcc gcgcttgcgg aggagttcgg
     1801 cgacacgccg tggtggcacg ggggcggctg cgtcgagtgg gcccgcacgg aggccgaccg
     1861 gtccgggcta cgtgcgcggg tcgagcggct gcggtcgtgg gggtacgcgg cggagtggct
     1921 gacccttcag caactgcggg cgctggagcc cgacatcgac ccggcggccg tgggggaggg
     1981 tcccatcgcc tacttcccgg aggaggggtg gatcgacccg gtcgtgtacg cgcacgcgat
     2041 gctcgaggct gcacagcgct ccggagcggc gctccgctgc ggggcgcggg tgaccgagat
     2101 ccgcgttgag ggcggccgcg tcaccggggt gcggaccgcc gccggcgagt cgttcggcgc
     2161 ggacgtcgtc gtcaactgcg ccggccggtg ggccgacgag gtcgcccgcc tggccggcct
     2221 gcggatcccg ctcgcgccca cggtcggcct gctggtcttc accccgcccg tggccacctg
     2281 cctccgccgg gttgtccggg ccccgcagtg ccacatgcgc ccggatggag ggggccggct
     2341 cgtgctgcgc acggaggatg ccgaccggca ggtgagcgcc gagaccgaac cgagcccgcg
     2401 cctcgccccc gcggaggacc tggtccgggg cgccgcccag gtcctgccgg ggatcggggg
     2461 gacggggccg gaggcggccc gaatcgcggt gaggccgatc ccttccgatg gctactctgc
     2521 ggtcggcccg atgccgggcg tgtcaggctc ttatctggtc gtgacgcaca gcggggtgac
     2581 gctcgcgccg ttccttggcc gggcggtggc ggaggaggtc acccggggcc gcacggtgcg
     2641 ggaactcgag aggttccgcc cgtccaggtt tgccgggccg accgggggcg ggtagatgtc
     2701 ccccggccgg cttccgcgat tcgaagggcc ggcccccgct cgcgcctgct acgtgggcca
     2761 gccagcggtt tcgctatttc tttcccgcat caatgagatc cttaatggcg tcgaggagct
     2821 tcctgtttgt gtccagcatc atcttcatcg tcgcggccat ttgctccatg ccgcccatca
     2881 tctctttctc catgctgctc atctgcatct tcttcatcga gtccatgtgg gtcatcgtgg
     2941 tggcgagctg tttctgggcc tgtgcctgag cggcctgggc cgcctggagt gcggcagtag
     3001 ccttgtcata ggagctttcc atctgagcgg aagcgggatg gatctgtccc cccaggcctg
     3061 ccacgagcag gcccatcacc atgatcatgg ccaccttctt cgaacgcaac atcatctttc
     3121 ccaccccttt cgaaccgatt cggtgttgcg acgatcttgc ggatcaacga gtcccgctgc
     3181 ccacgcgccg cgccgtccct cgggcctgca ccccctcgct tgcccccggc accacgtgcc
     3241 accgcaagcc taacccacct cgataaaggg gcgataaagg atctgtgaag gttgcgcaaa
     3301 gatctgaatc ctcgccaggg tccagcaaca acggccggga agatgtccgc atgacggctc
     3361 gggcaggggg gtg
//
LOCUS       DASQGH010000354         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46940, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000354 DASQGH010000000
VERSION     DASQGH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46940"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VEW91_06895"
     CDS             <1..589
                     /locus_tag="VEW91_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYM91345.1"
                     /translation="DPGADLGQPWTAGPVPAPQAIRGFHSVTLLEHAHQETDVLLTEV
                     LGCRLHQQIGHRRRYVWGPGGLATRVDVVSAPDAPRGQVAVGTIHHVAWRTPDDDAQR
                     AWRQRLVEEKISVSPVMDRQYFHSIYFHEPGGVLFEIATDGPGFVADEKPEALGTQLR
                     LPRWLEPERAALESSLPKLRVPRTGPPQDEVPSTP"
     gene            complement(611..1456)
                     /locus_tag="VEW91_06900"
     CDS             complement(611..1456)
                     /locus_tag="VEW91_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYM91346.1"
                     /translation="MERRRFGSAKREVAVIGQGTWEIERGDRATAIAALRRGLDLGAT
                     HIDTAELYGSGTAEEIVAEAIVGRRDEVFLVSKVLPENASRDGTIAACEKSLARLKTD
                     RLDCYLLHWRGRHPLEDTIAAFEHLQRKGKILSWGVSNFDVPDLEAARKIAGGNGPVC
                     NQVLYHLRDRAIEHAVIPWCEQHSVAVVGYSPFGSGRFPGPHTTGGRVLTHIAAAHHA
                     TPRQVALRFLVRRSSLFAIPKASSPEHSAENAGAGALRLTDAELSRIDKAFPLGPRPR
                     ELPML"
     gene            complement(1471..>1870)
                     /locus_tag="VEW91_06905"
     CDS             complement(1471..>1870)
                     /locus_tag="VEW91_06905"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HYM91347.1"
                     /translation="RRGTQMAQAEFVKALRDREEISITVKGRRSGREVRLPVWFTLEG
                     KTLWLLPMHGARTQWYRNVLADRMNTERAGRHALAVVAHPRRARTAVRRVVEQFRKKY
                     SAELVARYYDHSDAVVEVPLTPRTKRSVRR"
BASE COUNT          276 a          647 c          654 g          293 t
ORIGIN      
        1 agacccgggc gcggacctgg gccagccatg gacggccggg cccgtgcccg cccctcaggc
       61 gatccgaggg ttccacagcg tcacgctcct ggaacacgcc caccaggaga cggacgtgct
      121 gctgaccgag gtactcgggt gccgcctcca tcagcaaatc gggcaccggc gccggtatgt
      181 ttggggcccg ggcggcttgg cgacccgggt cgatgtggtg agcgctccag acgcgccgcg
      241 ggggcaggtg gcggtcggga ccatccacca tgtggcgtgg cggacaccgg acgacgacgc
      301 ccagcgggcg tggcggcagc ggctcgttga agagaagatc agcgtctcgc cggtgatgga
      361 ccgccagtac tttcattcga tctacttcca cgagcccggc ggcgtcctgt tcgagattgc
      421 gaccgacggg cccggcttcg tcgcggacga gaaacctgaa gcgctgggga cgcagctgcg
      481 actgccgcga tggctggaac ctgagcgcgc cgcgctcgaa tcgagcctcc ccaagctccg
      541 cgtgccgaga accggcccgc cccaggatga ggttccctcc acgccctgac ggacggggac
      601 gtggcagccg ctacagcatg ggaagctcgc ggggacgggg gccgagcggg aaggccttgt
      661 cgatccggct gagctcggcg tcggtcagcc gcagcgcccc ggcccccgcg ttttcggcgg
      721 agtgctcggg gctggaggcc ttggggatcg cgaagagcga ggaccgccgc accaggaatc
      781 ggagcgcgac ctggcggggg gtcgcgtggt gggcggcggc gatgtgcgtc aacacgcgtc
      841 ctcccgtggt gtgcgggccc ggaaagcgcc ccgaaccgaa cgggctgtag ccgaccacgg
      901 cgacgctgtg ctgctcacac caggggatca cggcgtgctc gatcgcgcga tccctcaggt
      961 ggtagaggac ctgattgcag acgggaccgt tcccgccggc gatcttccgg gccgcttcca
     1021 ggtcgggcac atcgaagttg ctcacgcccc aggagaggat cttccccttg cgctgaaggt
     1081 gctcgaaggc cgcgatcgtg tcctccagcg ggtgccggcc gcgccagtgc aagaggtagc
     1141 agtcgagccg gtcggtcttg aggcgagcga gcgacttctc gcaggccgcg atcgtcccgt
     1201 cccgagaggc gttctcggga aggaccttgg agacgaggaa gacctcgtcg cgtcgcccga
     1261 cgatcgcctc ggcgacgatc tcctcggcgg tgcccgatcc gtacaactcc gcggtgtcga
     1321 tatgggtcgc cccgagatcg aggccccggc gcaacgcggc gatcgcggtg gcgcggtcgc
     1381 cgcgctcgat ctcccaggtc ccttggccga tcaccgcgac ctcgcgcttg gcggacccga
     1441 accgacgccg ttccatgcct gtcctccgcc tcatctccgg acagaccgct ttgtccgcgg
     1501 cgtcagcggg acttcgacca cggcatccga gtggtcgtag tagcgggcga cgagttcggc
     1561 gctgtatttc tttcggaact gttccaccac tcgccggacc gccgtccgcg ctcgtcgggg
     1621 gtgcgccact accgccaagg cgtgccgtcc cgcccgctcg gtgttcatcc gatcggcgag
     1681 cacgttccgg taccattggg tgcgggcccc gtgcatcggg aggagccaca aggtcttccc
     1741 ctcgagcgtg aaccacaccg gaagcctcac ctcgcgcccg ctgcggcggc ctttcacggt
     1801 gatcgagatc tcctcgcgat cccggagcgc tttcacaaac tctgcctgtg ccatctgggt
     1861 gcccctcctg
//
LOCUS       DASQGH010000355         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21272, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000355 DASQGH010000000
VERSION     DASQGH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21272"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..>2206)
                     /locus_tag="VEW91_06910"
     CDS             complement(64..>2206)
                     /locus_tag="VEW91_06910"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HYM91348.1"
                     /translation="RDERGGHGNALAQPYTHMILPLATRRDKETQVAWGIADFRARFG
                     RDPEGMWLPETAVDTETLEVLSARGIRFTILAPRQAARVRAAGEEPWTDVTDGRIDPG
                     RPYLCRLPSGAAITLFFYDSPIARAVAFEGLLNSGEALANRLAAAFREDQNGPQLVHI
                     ATDGESYGHHHRFGEMALAHALRLIEERKTARLTNYGEFLALHPPDHEVQILEPTSWS
                     CAHGVERWRANCGCHVGHTGWNQEWRRPLRDALDWLRDELGRVYAEQAARVLRDPWAA
                     REAYVDVILRRSPEVLDAFFTAHGRADLRREERAGGLRLLEMQRHAQLMYTSCGWFFD
                     ELSGIETIQVMKHAARAIQLAEGFGDRLEAEFIGRLAAAKSNLPRWSDGARIYHRVVR
                     PSVVTLPRVVAHYAISALFEPYAETARIFSYTVRRLETLREERSGNALAIGLVVAASS
                     ITGEEQRAAFAVLHLGGHDVQCGIRPDPTAAWYAKVKAELTEAFLGTGPSDALRMLDE
                     HFKGSLHALPGLFMEARRAILERMTEEHLARVDEMYRALYEESRPLVALMRASDVPIP
                     PAILRAAEYTLGGELAEILGQAARVPLPPRAFEIARELDALEVKPDQAETELRLRRAA
                     EARAESLRIDPLGGDLDQVHRLLDLAAALGITMDLWQIQNTYHAAAQIHLDRLAEGHG
                     SDPGRVGEFWRLGERLHFNLNALRVPAGTPG"
BASE COUNT          293 a          765 c          794 g          354 t
ORIGIN      
        1 gttggtgtga tatggatctg aatgaattat ttttgtttgt ttagataaga cccgggcgcg
       61 ccgctagccc ggtgtcccgg ccggaacgcg cagcgcgttc aggttgaaat ggagcctctc
      121 gccgagccgc cagaactcgc cgacccggcc ggggtcgctt ccgtggcctt cggccaaccg
      181 gtcgaggtgg atctgggctg cggcgtggta ggtgttctgg atttgccaaa ggtccatcgt
      241 gatgccgagc gcggccgcta ggtccaggag gcggtgcacc tggtcgaggt cccctccgag
      301 cgggtcgatc cgcagggact ccgcgcgggc ttcggccgcc cggcgcaggc gcagttccgt
      361 ctcggcctgg tcgggtttca cctccagcgc gtcgagttcg cgcgcgatct caaacgcccg
      421 ggggggaagc gggacgcgcg ccgcctggcc caggatctct gcgagttccc cgccgagggt
      481 gtactccgcc gcccttagga tcgccggggg gatgggtacg tccgaggccc gcatcagtgc
      541 cacgagcggg cgggattcct cgtacagcgc ccggtacatc tcgtcgaccc gggcgaggtg
      601 ttcctccgtc attcgttcga ggatcgcacg gcgcgcctcc atgaagaggc cgggcagcgc
      661 gtggagcgac cccttgaagt gctcgtcgag catccggagg gcgtcgctcg gcccggtgcc
      721 gaggaaggcc tcggtgagct ccgccttcac cttcgcgtac caggcggcag tgggatccgg
      781 ccggatcccg cactggacgt cgtgtccgcc caggtgcagg accgcgaacg cggcccgctg
      841 ctcctccccg gtgatactcg acgccgccac gaccagcccg atggccagcg cgttgccgct
      901 tcgctcctcg cgcagggtct ccaggcggcg gaccgtgtag ctgaagatcc gcgccgtctc
      961 ggcgtacggc tcgaacaggg cgctgatcgc gtagtgggcg accacccggg ggagcgtcac
     1021 caccgatggg cggacgaccc ggtggtagat ccgggcgccg tcgctccagc gggggaggtt
     1081 gctcttcgcg gcggcgagcc ggccgatgaa ctcggcctcc agccggtccc caaagccttc
     1141 ggccagctgg atcgctcgcg cggcgtgctt catgacctgg atggtctcga tccccgagag
     1201 ttcgtcgaag aaccatccgc acgaggtgta catcagttgc gcgtgccgct gcatctcgag
     1261 gaggcggagc cccccggcgc gctcctcgcg ccggaggtcg gcccggccgt gggcggtgaa
     1321 gaatgcgtcc aggacctcgg ggctgcgccg aaggatgacg tcgacgtacg cctcccgggc
     1381 cgcccagggg tcccggagca ccctggccgc ctgctccgcg tagacccggc cgagttcgtc
     1441 ccgcagccag tccagcgcat cgcggagcgg ccgccgccac tcctggttcc atcccgtatg
     1501 cccgacgtgg cagccgcagt tggcccgcca ccgctcgacc ccgtgcgcgc acgaccagct
     1561 cgtcggctcc aggatctgga cctcgtggtc cggtggatgg agggccagaa actctccgta
     1621 gttggtgagc cgggcggtct tccgctcttc gatcagccgc agggcgtggg cgagcgccat
     1681 ctccccgaaa cggtggtggt gtccgtacga ctctccgtcc gtggcgatgt ggacgagctg
     1741 cgggccgttc tgatcctccc ggaacgccgc ggcgagccgg ttggcgagcg cctcgccgct
     1801 gttgagcaac ccctcgaacg cgaccgcgcg ggcgatcggg ctgtcgtaga agaacagcgt
     1861 gatcgccgcc ccgctcggca gccggcagag gtagggacgg cccgggtcga tgcggccgtc
     1921 ggtcacgtcg gtccacggct cctccccggc ggcacggacg cgggcggcct ggcgcggcgc
     1981 caggatcgtg aagcgaatgc cgcgggcgga caggacctcg agcgtctcgg tgtccacggc
     2041 ggtctcgggc agccacatcc cctcgggatc gcggccgaac cgcgcgcgga agtcggcgat
     2101 cccccaggcg acctgggtct ccttgtcccg gcgggtcgcg agcggcagga tcatgtgggt
     2161 gtagggctgc gcgagcgcgt tgccgtgtcc cccccgctcg tcccgg
//
LOCUS       DASQGH010000356         8801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21274, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000356 DASQGH010000000
VERSION     DASQGH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21274"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..865)
                     /gene="cofE"
                     /locus_tag="VEW91_06915"
     CDS             complement(107..865)
                     /gene="cofE"
                     /locus_tag="VEW91_06915"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HYM91349.1"
                     /translation="MPSATLIALEGIPDVRPGISLPALLVEALTASGLRPAAGDVLVV
                     AQKVVSKAEGSVVDLAEVSPGGEAHRLARATGKDPRLVEVILRESRQVVRVHAGVLIT
                     EHRLGLICANAGVDHSNVGLGPEVVCLLPRDPDASARAIRVALGAAFQTPVAVIISDS
                     HGRPHREGAVGVCIGADGLEPLVSLVGRPDRYGYTLRTSVEAVADELAAAATLLQGQC
                     DEGTPAVLIRGVPVLEGAGGAHRLLRDPTHDLFR"
     gene            complement(865..1512)
                     /gene="npdG"
                     /locus_tag="VEW91_06920"
     CDS             complement(865..1512)
                     /gene="npdG"
                     /locus_tag="VEW91_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HYM91350.1"
                     /translation="MTTVGVIGGTGHQGLGLAMRWAAARVPVVIGSRSPERAREAAAR
                     VRTATGGGPVDGMGNLQAAREAEIVVVTVPVGAHAETLAALAPALEGKVVVDTTVPLD
                     STFSHAVRLPEGSAAERAQRILGERARVVGAFHTVPASLLADLSRPIECDVLVCGDDA
                     AAKARVVEIAKPLGARVIDVGGLQQAHTLERMTALLIDLGRRVRRHDLGVRITGL"
     gene            complement(1517..2530)
                     /locus_tag="VEW91_06925"
     CDS             complement(1517..2530)
                     /locus_tag="VEW91_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM91351.1"
                     /translation="MTRVAFGLGGNLPVRQAVEYARYAEAQGFESCWVHEAYWNRDAS
                     SYLVAMAVGTRRLGLATGCINPHTRHPVLVASSLATLDELSGGRAVLGFGTGYPGRLD
                     EQGIAHDHPAAAMRESITLIRRLWQGEKLTYTGRRFSLRNAALAVRPAHPVPIYLAGW
                     GPLMLRIAGQLCDGYLARALESPASCRRLIGAVRAAATAAGRPLGAVDTAAYVLCAVS
                     GDRAAARAAMRRDPFVIYQFAVIDEAVLRESGVDPEVKRRIGEPFRRGDLEGASREIG
                     DALLDEFTLAGTADDVIARLEAYAQAGVQLPILQPIATTEPEVRRVIDVGREFARSRA
                     AAD"
     gene            complement(2527..3816)
                     /locus_tag="VEW91_06930"
     CDS             complement(2527..3816)
                     /locus_tag="VEW91_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYM91352.1"
                     /translation="MIESELRRRALPITPEAVVRQPLGSGAVALIREKRANPSVTILG
                     YIRAGAMHDPAGREGLALFTASMLTRGTASHTSQDLALVLDSLGASLNVRADLEGVTV
                     SARCLAEDVERILELAAEVILRPTFPPEEIEKQRGRIITGIRESRLDTRIAAEKAFRN
                     VAYPLRHPHHRTSEGEEESIAAVTRDDLVAFHRRFYRPDGFAVAVVGDVAPAGILERL
                     RGLWDGWRPVAEAETIIPPPVGPASSVQRTVVEIPGKAQADIVLGVPGFSRTSPDFYP
                     AMMADLILGRLGLMGRLGATVRDEEGLAYYVYSQAQAGFLAGPWTVRAGVSPRNVERA
                     VEGIVREIRGLQREPVRDGELVDAQNYLIGSLAVRLETDGGIAQALLEIELFDLGLDY
                     LLRYPTLIRSITPEAIGAAASRYLNLNGYTLATAVPA"
     gene            complement(3803..5143)
                     /locus_tag="VEW91_06935"
     CDS             complement(3803..5143)
                     /locus_tag="VEW91_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYM91353.1"
                     /translation="MEPTTRTVLPNGLVVLLREVHTAPVATFWIWYRVGSRNEVPGIT
                     GISHWVEHMLFKGTPTLAKGEFSRLINRHGGSWNGFTWKDFTAYFETLPAEHLSLGIR
                     IESDRIRNALFDPEEVQSERTVIISEREGAENSPEYALSEEVEAAAYRVHTYRHAVIG
                     YKSDLVAITRDDLVRHYQTYYTPNNAVVVAVGDFDTTALLGEIREAFEPIPPGPPAPP
                     VRSVEPPQEGERRVMVRRPGGAVPQLHMVFHAPAARDPEFFPALLLDGVLSGFKGPGV
                     FGGEAMGVRSSRLYRALVEQQLAVEAGSGFRPSLDPTLFEIGATLRPGVSPERAEGAV
                     LDELKRLGDEVVPAAELEKVLKQARAQWVYADDGVSRQAVLLGSSEIVADSTYLARFW
                     EGLTSVTPEGMRQVASRLFTDRNRTVGWYIPEAGQAQVRDGARPAAEAAAHDRV"
     gene            5307..6188
                     /locus_tag="VEW91_06940"
     CDS             5307..6188
                     /locus_tag="VEW91_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYM91354.1"
                     /translation="MVPAVQATQSLSGAVIGRLRKQVTHFYRIRTKTQALRFIRDLGF
                     CYAFTQGPGSLPCLFEALATRSDDRRWSWAWDWKEALPSEKRVFYGRVLGRKPTFISM
                     EFLPHFFALTGNVGEPDDYRRLYDGGRLSYLARVVYELTAARGPLTTRQIRAAVEPNR
                     RGSSATLLRALADLQMLFLLARIGETGDNPGNYAYVWDLFDRWLPGVVAQAAEISHRT
                     AAAAVLGRYVAIVGAPRPEDAAALFEWPPTVLATAIGDLLGRGAVVVAHGPGGEDRLL
                     TPAALKRLTTRRPTAIS"
     gene            complement(6172..6642)
                     /locus_tag="VEW91_06945"
     CDS             complement(6172..6642)
                     /locus_tag="VEW91_06945"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013336972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYM91355.1"
                     /translation="MPVEVGQQAPDATLVSTDRKQVRISELRGRTTVLAFFPAAFTGT
                     CTKEMCRFRDDHTRLDALAARVIGISADTPYVLAEWGKQLDLRQTMLSDFNHEAMRAY
                     GVYDGNFNGLLNGIARRSVFVLDKDGKVVYTWIADRPGVEPPYEEVEAAVKKSQ"
     gene            6750..7193
                     /locus_tag="VEW91_06950"
     CDS             6750..7193
                     /locus_tag="VEW91_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HYM91356.1"
                     /translation="MAATRRADVTWEGDLMSGGGVVTASASRAFSALPVSWASRTDTP
                     RGQTSPEELVAAAHASCFAMALSGGLAGAGKPPKKLEVSATVTFDKVEAGWRVISSAL
                     TVRGVVPGMDTAGFRKAADAAKDGCPISQALRGNVKLSVEATLVS"
     gene            complement(7186..>8801)
                     /locus_tag="VEW91_06955"
     CDS             complement(7186..>8801)
                     /locus_tag="VEW91_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYM91357.1"
                     /translation="RRLYGIPTARGVLGEALPTLDAVLEALHARYPGIRDRLCEPDGT
                     LREYIHVFVDACDLGRDPAAIEVPDGAEVTVVPSIAGGGVAVDEALRARIDAELPQTI
                     EDLRRLVRIPSVAAQRQGIPDTVRAVGDLLRGAGGRVTVLEHAGANPVVVGEFEGRSP
                     RTLLFYDHYDVQPAEPLDEWTVPPFDVTQRDEKLFGRGVADNKGDLMTRIAAIRSLRA
                     VRGGLPCRVKFLIEGEEEISSPHLGAITRAHTDLLKADACIWEYGERDSRERKHIVCG
                     MKGICYLQLEAKTAAVDLHSSLGAVFEGAAFRLVWALSTFKDQNGRVQIPGHYDRVRR
                     PTPAEEEVIRQIPPDVVEDVQKQVGVARTIGGVGGPEAVRQLLLTPTCTICGIWGGYT
                     LEGSKTVLPKVARAKVDFRLVPDQDPHEVAQNVRRHLDAGGFGDIEVTVLGAEHPWRT
                     NLADPFVGLVRDCVKEATGREVLVYPTSAGTGPAHDVGPVLGIPLVSAGSGYWNARAH
                     APDENVRASDFRETILLMAHILERFGRIS"
BASE COUNT         1305 a         3242 c         2952 g         1302 t
ORIGIN      
        1 cgccaccttc cacgtcatcc cggccggctg ggccgacccc gagaccgcgg ccacccctac
       61 caccaccatg ccgaccatca gggcaggcag cgccttcgtc atgcatctac ctgaacaagt
      121 cgtgggtggg gtccctgagg agcctgtgcg cccctccggc gccctccaac accggcacgc
      181 cccggatcag caccgccggc gtgccctcgt cgcactgccc ctggagcagc gtagccgccg
      241 cggcgagctc gtccgcgacc gcctcgaccg aggtccgcag cgtgtagccg taccggtcgg
      301 gccgccccac cagactgacg agcggttcca ggccgtcggc gccgatgcag acgcccaccg
      361 ccccttcccg gtgtggccgt ccgtggctgt cgctgatgat caccgccacc ggcgtctgaa
      421 acgcggcgcc gagcgccacc ctgatcgcgc gggccgatgc atccgggtcc cgcggcagca
      481 ggcagacgac ctcgggcccg aggccgacgt tgctgtggtc cacgccggcg ttcgcgcaga
      541 tgaggccgag tcggtgctcg gtgatcagca cccccgcgtg gacgcggacc acctggcggg
      601 attcccgcag gatgacctcg accagccgcg ggtctttgcc ggtcgcgcgg gcgagccggt
      661 gcgcctcccc gcccggtgag acctccgcga ggtcgacgac gctgccttcg gctttgctga
      721 cgactttctg agccacgacc aggacatcgc ccgcggcggg ccggagcccg gaggcggtga
      781 gcgcctcgac cagcagcgcg ggcagcgaga ttccgggacg aacgtcgggg atcccctcga
      841 gggcgatgag ggtcgcggag ggcatcagag ccccgtgatc cgcacgccaa ggtcgtgccg
      901 gcggacgcgc cggcccaggt cgatcagcag cgcggtcatc cgctcgagcg tgtgcgcctg
      961 ctgcagcccg ccgacgtcga tcacccgggc gcccagcggc ttcgcgatct ccaccacgcg
     1021 cgccttcgcc gcggcatcgt ctccgcagac caggacatcg cactcgatcg gccgggacag
     1081 gtcggcgagc aggctggccg gcaccgtgtg gaatgccccc accacgcgcg cccgctcccc
     1141 gaggatccgc tgcgcgcgct ccgcggccga cccctcggga aggcgcacgg cgtgcgagaa
     1201 ggtggaatcg aggggcaccg tggtgtcgac gacgaccttg ccctcgaggg cgggggcgag
     1261 cgccgccagg gtctcggcgt gcgctccgac cggaacggtc accacgacga tctcggcttc
     1321 ccgcgccgcc tgaaggttcc ccatcccgtc caccgggccg ccccccgtgg ccgtccgcac
     1381 ccgcgcggcc gcctcgcggg cccgctccgg cgatcgggag ccgatcacca ccggcacccg
     1441 ggcggcggcc cagcgcatcg cgagccccag cccctggtgc cccgttcccc cgatcacccc
     1501 gacggtcgtc acgccctcag tcggccgcgg cccggctgcg cgcgaactcg cggccgacat
     1561 cgatgacgcg ccgcacctcc ggctcggtgg tcgcgatcgg ttggagaatc ggcagttgga
     1621 ccccggcctg cgcgtacgcc tcgagccggg cgatcacgtc gtcggccgta ccggccaggg
     1681 taaactcgtc gaggagggcg tcgccgatct cccggctcgc gccctcgaga tccccgcgcc
     1741 ggaacggttc accgatccgc cgcttgacct cggggtccac gccgctctcg cgcagcaccg
     1801 cctcgtcgat cacggcgaac tggtagatga caaacggatc acggcgcatc gcggcgcgtg
     1861 ctgcggcgcg atcccccgac accgcgcaga gcacataggc ggccgtatcc accgccccca
     1921 gggggcgccc ggcggccgtg gcggccgccc gcacggcccc gatcaaccgg cggcatgacg
     1981 ccggcgactc cagcgcgcgc gccaggtagc cgtcgcacag ttggccggcg atccgcagca
     2041 tcagggggcc ccatccggcc aggtagatgg gaacgggatg cgccgggcgc accgcgagcg
     2101 cggcgttcct cagcgagaac cgccggccgg tgtaggtcag cttttccccc tgccagagcc
     2161 tccgaatgag ggtgatgctc tcgcgcatgg ccgcggccgg gtggtcgtgc gcgatgccct
     2221 gctcgtccag ccgtcccggg tagcccgtcc cgaaccccag cacggcccgc ccgccgctga
     2281 gctcatccag ggtcgcgagc gaggacgcca cgagcaccgg gtgccgcgta tgggggttga
     2341 tgcatcccgt cgcgaggccg aggcggcgcg tgccgaccgc catcgccacg agataggagg
     2401 acgcgtcccg gttccagtag gcctcatgga cccagcagga ctcgaacccc tgcgcctccg
     2461 cgtaccgggc gtactcgacc gcctgccgca ccggcaggtt gccgcccagc ccgaacgcga
     2521 cgcgggtcac gccgggaccg ccgtcgccag ggtgtatccg ttgaggttga ggtagcggga
     2581 ggccgccgcg ccgatggcct cgggggtgat gctgcggatc agcgtcgggt accggagcag
     2641 gtagtcgagg cccagatcga ataactcgat ttccagcagc gcctgagcga tccccccatc
     2701 ggtctccagc cgcacggcga gcgagccgat caggtagttt tgcgcatcca cgagttcgcc
     2761 gtcccggacc ggctcgcgct gcaacccgcg gatctcgcgg acgatcccct cgaccgcgcg
     2821 ctccacgttt cgcgggctga caccggcgcg caccgtccac ggacccgcca ggaatccggc
     2881 ctgggcctgg ctgtagacgt aatacgccag cccctcctca tcgcggacgg tggcccccag
     2941 ccgccccatc agcccgagcc ggccgaggat caggtcggcc atcatggcgg gatagaagtc
     3001 cgggctcgtc cggctgaatc ccgggacccc caggacgatg tccgcctggg cctttccggg
     3061 gatctcgacc acggtccgct ggaccgacga agccggcccg accggcgggg ggatgatcgt
     3121 ctccgcctcg gccacgggcc gccagccgtc ccagaggccc ctgagccgct ccaggatccc
     3181 ggcaggagcg acatccccta ccaccgcaac ggcgaacccg tcgggccggt agaagcggcg
     3241 gtggaacgcg accaaatcgt cgcgcgtcac cgcggcgatc gattcctcct cgccctccga
     3301 ggtccggtgg tggggatgcc ggagcgggta ggccacgttg cggaacgcct tctcggcggc
     3361 gatgcgggtg tcgagccggc tttcccggat cccggtgatg attctcccgc gctgtttttc
     3421 gatctcctcc gggggaaacg tcggtcggag aatcacctcc gccgccagct cgagaattct
     3481 ctccacgtcc tccgccagac accgcgcgga caccgtgacc ccttccagat cggcgcggac
     3541 gttgaggctg gcccccagcg agtccaacac gagggccaga tcctgcgagg tgtgggacgc
     3601 tgtcccgcgc gtgagcatcg aggccgtgaa gagcgcgagc ccttccctcc ccgcaggatc
     3661 gtgcatcgcg cccgcacgga tgtagccgag aatggtgacc gacggattgg cgcgcttctc
     3721 ccggatcagc gcgaccgctc cggagccgag cggctggcgg accaccgcct cgggggtgat
     3781 cgggagggcc cgcctgcgca gctcagactc gatcatgggc tgctgcctcc gcggccggcc
     3841 gggccccgtc ccggacctgg gcctgtcccg cctccgggat gtaccagccc accgtccggt
     3901 tgcgatcggt gaacagccgg gaagccacct gccgcatccc ctcgggcgtc accgacgtca
     3961 acccctccca gaaccgcgcg aggtaggtgc tgtccgccac gatctcgctg ctccccaata
     4021 gcaccgcctg ccggctgacc ccatcgtccg cgtagaccca ctgcgcccgg gcctgcttga
     4081 ggactttctc caactccgcc gccggcacca cctcgtcgcc cagccgcttg agctcgtcga
     4141 gcaccgcgcc ttcggcgcgc tcgggcgaga ccccgggccg cagcgttgcg ccgatctcga
     4201 agagggtggg gtcgagcgag ggacggaacc cactgccggc ctcgacggcc agctgctgct
     4261 ccaccagcgc ccggtacagc cggctgctgc gcacccccat cgcctcgccg ccgaacacgc
     4321 ccgggccctt gaaacccgag agcaccccgt cgagcagcag ggcggggaag aactcggggt
     4381 ctctggcggc gggcgcgtgg aacaccatat gcaactgggg gaccgcgcca cccggccggc
     4441 gcaccatcac ccggcgctcc ccctcctggg gaggctcgac gctgcgcaca ggcggagccg
     4501 gcgggccggg cgggatcggc tcaaacgcct cgcgaatctc gccgagcagc gccgtggtgt
     4561 cgaagtcccc aaccgctacc acgaccgcgt tgttgggcgt gtagtacgtc tggtaatgcc
     4621 ggaccaggtc gtcccgggtg atcgccacaa gatcgctctt gtacccgatg accgcgtgcc
     4681 ggtaggtatg gacccggtac gccgcggcct ccacttcctc ggacagcgca tactcggggc
     4741 tgttctctgc tccctcccgc tcggagatga tcaccgtgcg ctcgctttgg acttcctcgg
     4801 gatcgaagag cgcgttacgg atgcggtccg actcgatgcg gatgccaagc gagagatgtt
     4861 ccgcgggcag ggtttcaaag tacgccgtga aatccttcca ggtgaagcca ttccaactgc
     4921 ctccgtggcg gttgatgagg cgggagaact cccctttggc gagcgtgggc gtgcctttga
     4981 agagcatgtg ctcgacccag tgggagatcc cggtgatccc ggggacctcg ttgcggctcc
     5041 cgacccggta ccagatccag aacgtcgcca ccggggcggt gtgcacctcg cgcagcagca
     5101 cgaccaggcc gttgggcaga acggttcgag ttgtgggctc catgaccggg ctcctctcga
     5161 gcctccattg cttgcctgcg gccggggact gccctgccgc aggcacggca aagccgggta
     5221 ccgacttcgg ggagccttcc actcttctgt cccgccaccg gtcccccctg ccacagtaac
     5281 ggccggatta gggccccgct tccccggtgg tacccgccgt ccaagccacg cagagcctct
     5341 caggcgctgt aatcgggcgc ctgcgcaagc aggttacgca tttctaccgt attcgtacga
     5401 aaacgcaggc gctgcggttc attcgggacc tcggcttctg ttacgcgttc acccagggcc
     5461 cagggtccct cccctgcctt ttcgaggcgc tcgccacccg aagcgatgac cggcgctggt
     5521 cgtgggcgtg ggactggaag gaggccctcc cgtcggagaa acgggtgttt tacggccggg
     5581 tgttgggaag gaagcctacc ttcatttcaa tggagttcct gccgcacttt tttgcgctca
     5641 ccggaaacgt cggggaaccc gacgactacc ggcgcctgta cgacggcgga cgtttgtctt
     5701 acctggcacg ggttgtctac gagctcaccg ccgcgcgggg accgctgacc acccggcaga
     5761 tccgggccgc ggtcgaaccc aaccggcggg ggagcagcgc gacgctcctg cgcgcgcttg
     5821 ccgacctcca gatgctcttc ctgctggcgc ggatcggcga aaccggtgac aacccgggga
     5881 actatgccta cgtgtgggac ctgttcgacc ggtggctccc cggggtggtg gcccaggcgg
     5941 cggagatctc ccaccggacc gcggctgccg ccgtcctggg gcggtacgtg gcgatcgtcg
     6001 gggctccacg cccggaggac gcggccgccc tgttcgagtg gccgccaacc gtcctggcca
     6061 ccgcgatcgg tgacctgctc ggccggggcg cggtcgtggt cgcccacggg ccgggcgggg
     6121 aagaccggct cctgaccccc gccgctctga aacgcctcac cacgcggagg cctactgcga
     6181 tttcttaacg gccgcctcca cctcttcgta cggcggctcc acgcccggcc ggtcggcgat
     6241 ccacgtgtag acgaccttgc cgtccttgtc gagcacgaac acggaccgtc gggcgatccc
     6301 gttcaagagg ccgttgaagt tgccgtcgta cacgccgtag gcccgcatcg cctcgtgatt
     6361 gaagtcgctc agcatcgtct gcctgagatc gagctgcttg ccccactccg ccagcacata
     6421 cggcgtgtcg gcgctgatcc cgatgacccg ggcagcgagc gcgtcgagcc gcgtgtgatc
     6481 gtcgcgaaac cggcacatct ctttggtgca cgtcccggtg aaggccgccg ggaagaacgc
     6541 gagcacagtg gtcctgccgc gcaactcgct gatccggacc tgcttgcggt cggtgctcac
     6601 gagcgtggcg tctggcgcct gctgtcctac ctcgaccggc atcgtgaacc tcccttcgag
     6661 aagacccgca gagggttctg ccaccccgcc catgaacttg gtacgccaga gttgccgctt
     6721 ttcatcccaa cgtaccgagg aggcgagcga tggcagcgac caggcgcgcc gacgtcacct
     6781 gggaaggcga tctgatgtca ggaggggggg tcgtgaccgc cagcgccagc cgggcgttca
     6841 gcgcgttgcc ggtaagttgg gcctcgcgca ccgacacacc ccgggggcag accagcccgg
     6901 aggaactggt cgccgccgcg cacgccagct gctttgcgat ggcgctctcc ggcggcctgg
     6961 ccggcgccgg gaagccgccg aagaaactgg aggtgagcgc gaccgtcacg ttcgacaagg
     7021 tcgaggcggg atggcgggtc atctccagcg cgctcacggt gcgcggcgtg gtccccggca
     7081 tggacaccgc ggggttccgg aaggcggccg acgccgccaa ggacggatgc ccaatctcgc
     7141 aggcgctccg ggggaacgtc aaactcagcg tggaggcgac gctggtcagc tgatcctccc
     7201 gaaccgctcc aggatgtggg ccatcaggag gatcgtctct cgaaagtcgc tcgcgcggac
     7261 gttctcatcc ggagcgtgcg cgcgggcgtt ccagtagcct gacccggcgc tgacgagcgg
     7321 gatcccgagg acggggccga cgtcgtgggc cgggcccgtc ccggccgagg tcgggtacac
     7381 gagcacctcc cggcccgtgg cctctttgac gcagtcccgc accaagccga caaacgggtc
     7441 cgccagattg gtccgccagg ggtgctccgc gcccaggacg gttacctcga tgtccccaaa
     7501 gccgcccgcg tccaggtgcc ggcggacgtt ttgcgcaacc tcgtggggat cctggtccgg
     7561 gacgagccgg aagtcgacct tggcgcgcgc caccttgggg agcacggtct tggagccctc
     7621 gagcgtatac ccgccccaga tgccgcagat cgtgcacgtc ggcgtcagca gcaactggcg
     7681 gaccgcctcc gggccgccca cgccgccgat cgtcctggcc acccccacct gtttctggac
     7741 gtcctccacg acgtccgggg ggatctgccg gatgacctcc tcctccgcgg gggtgggccg
     7801 ccggacccgg tcgtagtggc cggggatctg cacacggccg ttctggtcct tgaaggtgct
     7861 gagcgcccag accaggcgga acgccgcgcc ctcgaagacg gccccgagcg aagaatgaag
     7921 atcgaccgct gcggtcttcg cctccaactg gaggtagcag atccccttca tcccgcacac
     7981 gatgtgcttg cgctccctcg agtcgcgctc gccgtactcc cagatgcagg cgtccgcctt
     8041 gaggaggtcg gtgtgggcgc gcgtgatcgc acccaggtgg gggctgctga tctcctcctc
     8101 cccttcgatg aggaacttca cccggcacgg gagcccgccg cgaacggccc ggagtgagcg
     8161 gatcgcggcg atcctcgtca tgaggtcccc tttgttgtcc gccaccccgc ggccgaacag
     8221 cttctcgtcg cgctgggtga catcgaacgg cgggacggtc cactcgtcga gcggttcggc
     8281 gggttggacg tcgtagtggt catagaagag gagggtacgc ggggatcgcc cctcgaactc
     8341 ccccacgacc accggattgg cccccgcgtg ctctagcacc gtaacccggc cgcccgcacc
     8401 gcggaggagg tcgcccaccg cccggacggt gtccgggatc ccctgccgct gcgcggcgac
     8461 ggatgggatc cgcaccaacc ggcggagatc ctcgatcgtc tgcggcaact ccgcgtcgat
     8521 ccgcgccctc aatgcttcgt ccaccgctac gcctcccccc gcgatcgacg ggacaacagt
     8581 gacctctgcg ccatccggca cctcgatcgc ggcagggtcg cgaccgaggt cacaggcatc
     8641 gacaaagacg tggatgtact ctcggagcgt tccatccggc tcgcagaggc ggtcgcggat
     8701 cccgggatac cgggcgtgga gcgcctccag caccgcatcg agcgtcggca gcgcctcccc
     8761 gaggacgccc cttgcggttg gaatcccgta gaggcgccga a
//
LOCUS       DASQGH010000357         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44029, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000357 DASQGH010000000
VERSION     DASQGH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44029"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1207..1279)
                     /locus_tag="VEW91_06960"
     tRNA            complement(1207..1279)
                     /locus_tag="VEW91_06960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1244..1246),aa:Lys,seq:ctt)
     gene            complement(1349..>1823)
                     /locus_tag="VEW91_06965"
     CDS             complement(1349..>1823)
                     /locus_tag="VEW91_06965"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HYM91358.1"
                     /translation="DLEVETLAAGVPFHHVPVESAHRERAEAAMLDLLAGNADLLILA
                     RYMQVLSGDFLERVGMPAINIHHSFLPAFPGADPYSRARERGVKLIGATAHYVTEELD
                     DGPIIDQDVVRVDHRQSARDLERVGRDVERLVLARAVSLHLDDRVIVDGRRTVVF"
BASE COUNT          310 a          539 c          653 g          321 t
ORIGIN      
        1 gggtgtatcc tgcggcgcgc gcctcgcgtg tcgagccggt actcgcattg gctcggtgag
       61 gccggcgggg ctcgtggcga catcggctta gatctcagag tgcgcccaca ctggcgagtt
      121 gcgtagtcag aggtgccaca catcggggtg gcgcttgtcg ggagagcgag ttagcttcgg
      181 tggtgaccat gccaggtgtg cgtacctggc attggagcgc cccacgaacg actattgaag
      241 gaggccacgt catcatggta gggcaagcaa gtctcggcag ggcgaggcgg ttggttgcca
      301 cccttgcact cattgtcacg gccgcagcca cggtcggggt cgtcgtcgga ccaagcgtgg
      361 cacaggctgc tcacggttgc gtagcttcat atggctgcgg gtgggtcgac ggatcatacg
      421 gtaccccgat aggtaaatgg agtgaaaaca cgagtaactt cgcgaacttt ggacaatcgc
      481 gatgccaaac aggaacgtgg aacgactgca tttcctcgct ctggaacgaa ggtacgtcgt
      541 gcaacgccat atggtggacg aactccggat acggtgggag tggataccac aacaaccttg
      601 gcactggtac aggggtgctg tctggtggtt ggaacgatga attctcgtca ctgaagtggt
      661 gccaatactg aggcccgtgt cacggacgtt agtgtagaca accgcgaggg accttagact
      721 ccgatttaga cgacgaatat ggtgcctctg gtgctgttgc gccgtagggt ggccaggtgt
      781 gctgcgcggc gcagcagcac gaggaccagt gaagttcagc ctcgccgtcg gatcggtgct
      841 gaagttgctt gatgcggcgt gcccgcgctc gcgcgcatct acggcctgga gccggagggg
      901 agaggacgcc gtagcggccc gcgagccccg gaaccaatct gcgcgtaggg ctgtcaatgt
      961 tgcccgccgg ggccgggcct tggccgaggt ccagcgcacc gcacgggctc gtccctggca
     1021 gccggtgccg tggtacttcc cgtggtactt cagtgagcga atctgatctg atctgagcgc
     1081 acctgagatc ggctagcaag cctcatatcg cctgagctga ggtctggcgg cgatttcgat
     1141 cacaacctcg cgagagcggc cgccggagtc ttcggcatgc cggcggttcg ataactgtca
     1201 acgtgatggg ccgtccgggg atcgaacccg gaaccttggg cttaagaggc ccctgctctg
     1261 ccggttgagc taacggcccg ggagggcgcc ggcgaggtcg gccggcttgg ggaccgattc
     1321 tacccgagcg ggcgccgcgg ccgcgggctc agaagacgac cgtgcgccgc ccgtcgacga
     1381 tcacccggtc gtcgaggtgc agcgagacgg ccctggccag caccagccgc tccacgtccc
     1441 gcccgacgcg ctccagatcg cgggcgctct ggcgatggtc cacgcggacg acgtcctggt
     1501 cgatgatggg gccgtcgtcg agctcctcgg tgacgtaatg ggcggtggcg ccgatcagct
     1561 tgacgccgcg ctcgcgcgcc cgactgtagg ggtcggcgcc ggggaaggcg ggcaggaagg
     1621 agtggtggat gttgatcgcg ggcatgccca cccgctcgag gaagtcgccg ctgaggacct
     1681 gcatgtagcg agccaggatc agcaggtcgg cgttgccggc gagcagatcc agcatcgccg
     1741 cctcggcccg ctcccggtgg gcgctctcga cggggacgtg atggaagggc acgcccgccg
     1801 ccagcgtctc gacctcgagg tcg
//
LOCUS       DASQGH010000358         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41174, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000358 DASQGH010000000
VERSION     DASQGH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41174"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VEW91_06970"
     CDS             <1..407
                     /locus_tag="VEW91_06970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM91359.1"
                     /translation="SFAIVDSEKIHKDVTYEPPLVIAFGVDKHSVGAKTVTMGRTRIP
                     PKGRNQAHYHSCEASFFIRKGTLRLFMGKERKEYTVTENQFVYIPPGVIHGLLNLSDT
                     DTAELIFTYGNCPSKDDAGTVFVEKSWVTEPR"
     gene            438..908
                     /locus_tag="VEW91_06975"
     CDS             438..908
                     /locus_tag="VEW91_06975"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91360.1"
                     /translation="MRSARGNRILVTAGVVACALFGVGPIASGQGPAQITLATVNNMN
                     HVPQFVAVEKGLYVKHGVDVKLRVFNDGASATRAVQAGEAQMCTIGNSTLSAGWNAGV
                     RMVAVSVVMGDATRVYYDDPLAIVARSGSGIRRLHIEDLAGKRVGMIIGGTGEE"
     gene            918..1475
                     /locus_tag="VEW91_06980"
     CDS             918..1475
                     /locus_tag="VEW91_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91361.1"
                     /translation="MLAKEKVPPNRINFVNVPSPDHVSVLRNGGVDAEVVWEPYGTMI
                     LQQIPGAYVVLRGGGYLGWDLYLMGPEEFVKRNPRVVDGVVRGFVEAAWYVRQHPGES
                     AQIATRWIEGLDPTAAKKAITYMDFDPRFSANIFQAQAVVDRVMLDRGRIKQAADLSK
                     AIDTSFLDRVMREVPGMFADLKPVH"
     gene            1541..>2333
                     /locus_tag="VEW91_06985"
     CDS             1541..>2333
                     /locus_tag="VEW91_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91362.1"
                     /translation="MQAMRRARISLAVGALLMVVGLAPAVGQVPRQFTLATVNNMNHL
                     PEFVGVEKGIFVKHGIDLKLKVLNSGAEVMRAFQGGDAQFITMSPTTMAAAYNAGVRV
                     VAVVVVMGDPTRVYYDEMFAITGRPGSGIRRLHVEDLVGKRVGMVLGGTGEAYLRAVL
                     ARQKIAPDRITFVNVPAPSHVSVLRDGGVDAEVTWEPYGTLILQQVPQSYVVLRGGGY
                     IGYDLFVGTAEDFSKKNPDLLQQLVEGFAESEWYVRHHQGEAAQIA"
BASE COUNT          411 a          696 c          843 g          383 t
ORIGIN      
        1 ccagctttgc catcgtcgat tccgagaaga tccacaagga tgtcacctac gagccgccgc
       61 tggtgatcgc cttcggcgtg gacaagcaca gcgtcggagc gaagacggtc acgatgggcc
      121 gcacccgaat cccgcccaag ggaaggaacc aggcgcacta ccactcctgt gaagcctcgt
      181 ttttcatccg caaggggacg ctccggctgt tcatggggaa ggagcgcaag gaatacacgg
      241 tcacggagaa ccagtttgtc tacatcccgc cgggagtcat ccacggcctc ctcaacctga
      301 gcgacaccga cacggccgag ctgatcttca cctacgggaa ttgcccgagc aaggacgatg
      361 ccggaacggt gtttgtggag aagtcctggg tgaccgagcc tcggtagcga tcggacggcc
      421 ggcatcgggg agggaccatg cgaagcgcgc gcgggaaccg gatcctggtc acggcggggg
      481 tggtggcgtg cgctctgttc ggcgtcggtc cgatcgcctc ggggcagggc ccggcacaga
      541 tcacgctcgc tacggtgaac aacatgaacc acgtcccgca gttcgtggcg gtcgagaagg
      601 gactgtacgt caaacacggc gtggacgtca agctcagggt cttcaacgac ggcgcctcgg
      661 cgacgcgcgc ggtgcaggcg ggcgaggcgc agatgtgcac gatcggcaac tccaccctgt
      721 cggcggggtg gaacgcgggg gtccggatgg tcgcggtgtc cgtcgtcatg ggggatgcca
      781 cgcgggtgta ttacgacgac ccgctggcga tcgtggcccg gtccgggagc ggcatccggc
      841 ggctccacat cgaggatctg gcagggaaac gggtgggcat gatcatcggc gggaccggcg
      901 aggagtaatt ccgcgcggtg ctcgccaaag agaaggtccc cccgaatcgg atcaacttcg
      961 tcaatgtgcc ctccccggat cacgtgtcgg tcctccggaa cggcggcgtc gatgccgagg
     1021 tggtctggga accctacggg acgatgatct tacaacaaat ccccggcgcc tacgtcgtgc
     1081 tccggggggg cgggtacctc gggtgggatt tgtacttgat ggggcccgag gagttcgtca
     1141 agcggaaccc gcgcgtggtg gacggtgtcg tgcgtggctt tgtggaggcc gcctggtatg
     1201 tccgccagca cccgggggag tccgcgcaaa tcgcgacgcg gtggatcgag gggctcgatc
     1261 ccaccgcggc caagaaggcg atcacctaca tggatttcga tccccggttc agcgcgaaca
     1321 tctttcaggc acaggcggtc gtcgaccggg tcatgcttga ccgggggcgg atcaagcagg
     1381 cggcggacct gtccaaggcg atcgacacga gctttctcga ccgggtcatg cgagaggtcc
     1441 ccgggatgtt cgccgacctc aagccggtcc actgagggac gacggctggg gcgatggcaa
     1501 ggcccccggc ggtgagatgg gccggcgcaa aggagctgtg atgcaggcga tgcgaagggc
     1561 cagaatcagc ctcgctgtgg gggcgctcct gatggtcgtc gggctggcgc ccgccgttgg
     1621 gcaggtgccc cgtcagttca ccctggcgac ggtcaacaac atgaatcacc tgccggagtt
     1681 cgtcggggtc gagaagggga tcttcgtcaa gcacgggatc gacctcaagc tcaaggtcct
     1741 gaactcgggc gccgaggtga tgcgggcctt ccagggcggg gacgcgcagt tcatcacgat
     1801 gagcccgacg acaatggcgg ccgcttacaa cgcgggggtc cgggtcgtgg cggtcgtcgt
     1861 ggtcatgggc gatccgacgc gggtgtacta cgacgagatg tttgcgatca ccggtcggcc
     1921 gggcagcggg atccggcgcc tccatgtgga ggatctggtg gggaagcggg tcggcatggt
     1981 cctgggcggc accggcgagg cctacctccg ggcggtcctg gcccggcaga agatcgctcc
     2041 tgaccggatc acgtttgtca acgtccccgc ccccagtcac gtctcggtcc tccgggacgg
     2101 cggggtcgat gccgaggtga cgtgggagcc gtacgggacg ctgattttgc aacaggtgcc
     2161 gcagagttac gtcgtcctcc ggggcggcgg ctacatcgga tacgacctgt tcgtggggac
     2221 cgccgaggac ttttcgaaga agaaccccga cctcctgcag caactcgtcg aggggttcgc
     2281 ggagtccgag tggtacgtcc gtcatcacca gggcgaggcg gcgcagatcg cga
//
LOCUS       DASQGH010000359         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32794, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000359 DASQGH010000000
VERSION     DASQGH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32794"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1651
                     /locus_tag="VEW91_06990"
     CDS             164..1651
                     /locus_tag="VEW91_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYM91363.1"
                     /translation="MRRVLIANRGEIAVRIARACRELGLETVAAYSEADRDALHVRVA
                     DAAVAVGPADPRESYLNIGRLVDAARAAQADAVHPGYGFLAESPAFAAAVQDAGLTFV
                     GPPAGVIARLGDKVAARRLAVGAGAPVIPGYDEPQATDDALAAAARRLGLPVMIKAAG
                     GGGGRGMRLVEREEDLRPSLAAARREALAAFGSGDLLLERHLPEVRHIEVQVLADQRG
                     TVATLGERECSVQRRHQKLIEEAPSPGASPGLRATLEEAAASVARAAGYVNAGSVEFL
                     VEPSGQFYFLEVNTRLQVEHPVTEWATGVDLVKSQLLIAAGTPLPGGRAELRGHAIEC
                     RVYAEDPAHGYAPSPGPILALEEPAGPGVRVDSGLRAGWRVPQHYDPLLAKVIVWDQT
                     REAAIGRMLEALRQYVILGCGTNLNFLQDVLRHDAFRRAETTTRFLERHFARWEPEIP
                     ALALAAAACAEALTIGVRGSLRPEEQGASDPWDRLGRWRMGTAHG"
     gene            1644..2162
                     /locus_tag="VEW91_06995"
     CDS             1644..2162
                     /locus_tag="VEW91_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYM91364.1"
                     /translation="MDELTLVVRGHPVRCVVERAGEEFVVRTGEATYRIRLVELEPGV
                     LRVVAEGRTQTARIARDDTRRFLYLDGWTLEYTVATSGEPGSGVPHAAHEDLTAPMPG
                     GVTQVLVRRGERVTVGQPLVIVEAMKMEHVIRAPRPGRITAVRVREGDQVDGGAVVVE
                     IDWGSPPEGEPR"
     gene            2159..>2491
                     /locus_tag="VEW91_07000"
     CDS             2159..>2491
                     /locus_tag="VEW91_07000"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016714289.1"
                     /note="catalyzes the formation of acetoacetate and
                     acetyl-CoA from 3-hydroxy-3-methylglutaryl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYM91365.1"
                     /translation="MSALGTVPRRVRVVEVGPRDGLQNERATLGVEAKVEFITHLAAT
                     GLPVIEAGAFVRPDRVPQMADTDEVFRRLRGVRGPVLSALVPNRQGMERARGCDVREV
                     AVFTGASEA"
BASE COUNT          338 a          837 c          981 g          335 t
ORIGIN      
        1 ccaaatatga ggaagagggc aacgcctact acagcacggc gcgcctctgg gacgacggga
       61 tcatcgatcc ggccgagacc cggacggtgc tcgggctcgc gctgtccgcc gcgttgaacg
      121 cgcccatcaa aggcatgcgc ccgggcgtgt tccggatgta gcggtgcgcc gggtcctcat
      181 cgcgaaccgc ggggagatcg cggtgcggat cgcgcgcgcg tgccgcgagc tcggactgga
      241 gacggtcgcc gcgtactcgg aggccgaccg cgatgcgctg cacgtgcggg tggccgatgc
      301 ggcagtagcg gttgggcccg cggatccccg ggaaagctac ctcaacatcg gacgcctcgt
      361 cgatgcggcg cgggctgcgc aggcggacgc ggtgcatccg gggtacggct tcctggccga
      421 gagcccggcg ttcgccgccg cggtccagga cgcggggctg acgttcgtcg gcccccccgc
      481 cggggtgatc gccaggttgg gcgacaaggt ggccgcccgg cgcctcgcag tcggcgcggg
      541 cgcaccggtg atcccgggct acgacgagcc gcaggccacc gatgacgcgc tcgcagcggc
      601 ggccaggcgg ctggggctcc cggtgatgat caaggccgcc gggggcgggg gggggcgcgg
      661 gatgcgcctc gtggagcgcg aggaggacct gcgtccctcg ctcgcggcgg ccaggcggga
      721 agcgctcgcc gcgttcggct cgggcgacct cctcctggaa cgccacctgc cggaggtccg
      781 ccacatcgag gtgcaggtcc tcgcagacca gcggggcacc gtggcgaccc tgggcgagcg
      841 ggagtgcagc gtccagcggc ggcatcagaa gttgatcgaa gaggccccgt ccccgggcgc
      901 gagccccggg ttgcgggcca ccctcgagga ggcggccgcg agcgtggccc gcgcggcggg
      961 atacgtcaac gccggaagcg tcgagtttct cgtcgagccc tccgggcaat tctacttcct
     1021 cgaggtcaac acccggctcc aggtcgaaca cccggtcacg gagtgggcga ccggggtgga
     1081 tctggtgaag tcgcagctcc taatcgccgc ggggacgccc ctcccaggtg gccgggcgga
     1141 gttgcgcggc cacgcgatcg agtgccgggt ctatgccgag gatcccgctc acggctacgc
     1201 gccctccccg ggcccgatcc ttgccctgga ggaaccggcc ggtccggggg tccgcgtcga
     1261 ctccgggttg cgcgccggat ggcgggtccc ccagcactac gatcccctct tggcgaaggt
     1321 catcgtttgg gaccagaccc gcgaggcggc gatcggccgg atgctggagg ccctcaggca
     1381 gtacgtcatc ctggggtgcg gcacgaacct caacttcctg caggacgtcc tgcggcacga
     1441 cgcgttccgc cgtgcggaga cgacgacccg gttcctcgag cgccacttcg cccggtggga
     1501 gccagagatc ccggcgctcg cgctggcggc cgcggcgtgc gccgaggccc tcaccatagg
     1561 ggtgcggggg agtttgcgac cggaggagca gggggcctcc gatccgtggg atcgcctggg
     1621 gcgctggcgg atggggacgg cgcatggatg agctgaccct ggtcgtgcgg ggacacccgg
     1681 tgcggtgcgt ggtggagcgc gccggggagg agttcgtggt gcggaccggc gaggcgacct
     1741 accggatccg gctggtcgaa cttgagccgg gtgtgctgcg ggtggtggcc gagggccgca
     1801 cgcagaccgc gcggatcgcc cgcgacgaca cccggaggtt cctgtacctc gacgggtgga
     1861 ccctcgagta cacggtggcc acatccggcg aacccgggtc cggcgtcccg catgcggcgc
     1921 acgaggacct gactgccccg atgcccgggg gcgtgaccca ggtcctcgtc cgccggggcg
     1981 agcgagtgac cgtgggccag ccgctcgtga tcgtcgaggc gatgaagatg gagcacgtca
     2041 tccgcgcgcc ccggccaggc cggatcaccg cggtacgcgt ccgcgagggc gatcaggtcg
     2101 atgggggggc ggtcgtggtg gagatcgact ggggatcgcc tcctgaagga gagccacggt
     2161 gagcgcgtta ggcacggtcc cccggcgcgt ccgggtggtc gaagtcggcc cccgggatgg
     2221 actgcagaac gagcgcgcca cgctcggggt cgaggccaag gtcgagttca tcacccacct
     2281 cgcagccacc ggactccccg tgatcgaggc gggggcgttt gtccgtcccg accgcgtgcc
     2341 gcagatggcg gacaccgacg aggtcttccg gcgcctgcgc ggcgtccgcg gcccggtgct
     2401 ctccgccctc gtcccgaacc ggcaggggat ggagcgggcg cgtgggtgcg acgtgcggga
     2461 ggtggcggtg ttcaccgggg ccagtgaggc g
//
LOCUS       DASQGH010000360         9966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_46954, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000360 DASQGH010000000
VERSION     DASQGH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46954"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..755
                     /locus_tag="VEW91_07005"
     CDS             147..755
                     /locus_tag="VEW91_07005"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYM91366.1"
                     /translation="MTYDARRETILRAAGALFASKGYERTSIRDVARASGMSVAGLYY
                     YVRSKEELLYHICDRSFSTILEALDRGLAHTTDPRERVRVLIRNHLSHFLRDMNAIKV
                     ITRELGALDGVHATRIAALRRRYYATCRAVLEGLGTHPVAARLAAMSLFGMINWIHMW
                     YRPEVDGDADHLADGMARIFLDGYGTPSGGTTTWSRPMATSR"
     gene            725..1501
                     /locus_tag="VEW91_07010"
     CDS             725..1501
                     /locus_tag="VEW91_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYM91367.1"
                     /translation="MVAADGHVTLTRDGRAARIVLDRPPLNVLTLEMIQELTAICDRL
                     AEAPEIVIVTLAAAGGRAFCAGVDVAAHAPDRAEPMLAAFEAMAERLLALDPILIAAV
                     SGAALGGGWELALLCDFVVASERAMFGVPEVKLAALPPVAAAILPLLVGHLRALEFVV
                     TGRQISAHEAQGLGLVSRVASAENVETATRELVATLLSQSGPAIRAAKAAVVAPRRDA
                     ICRSLRAATEVYRSRLLPTQDAAEGIAAFLEKRAPAWKHV"
     gene            1517..2683
                     /locus_tag="VEW91_07015"
     CDS             1517..2683
                     /locus_tag="VEW91_07015"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HYM91368.1"
                     /translation="MKAEQELGELIERCRTLAEDPGYPAVRRWLEANPCGKVLGHFQV
                     YFPEEIAHAAGMLPVKILGAGSAIQIRKADARIAAFVCSIIRSSLELALTDRLNFLSV
                     FVTPPICDAARNACAVWVRNLPNINCQVLYLPQNAASPHAASFLRGEYRRIAGVIEET
                     TGQPITDEAMRQSIALFNENRRLLRELYRVKRETPWLLSAVEAYTLVRAGGLMPREEH
                     NPLLRRVLDLVPQRQAKRQDRIRVVFEGGYCEQPPVDMLAVIQDACYIVDDDLLIGMR
                     WLTGDVPTEGEPLASLARAYLDGSSYSPVQHDPRKPKPEMLLQRIRESHADAAIVAAP
                     KMCEPGLEEQVNHIRALEQAGIPHLVMEFEEKMTVFEQMRMEVETFAESLLLFA"
     gene            2715..4001
                     /locus_tag="VEW91_07020"
     CDS             2715..4001
                     /locus_tag="VEW91_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HYM91369.1"
                     /translation="MTTETPTRIGSIRDEGRRLLERWWQEMGAADQAGRPTAYVFVMG
                     SLDEILRVFDLPINCPEITSLQTAVRGQSLQYLQAAEDYGYSPDICGYVKTDVGLQLK
                     DRQHPNGKVPKPALAIATNMCNTYIKWAEIWEQMYHCPIFVLDLPAWRGTGAAPGVDS
                     ETFQNDRRYVEGQLRDLIALCERLTGKAFDIDRLREVMGEVNRMAASYHEVLALNKHR
                     PAPFSAIREGITFQGISNLYRGSPEGSRFFGVAREEIQERIRRGMGTLEEKYRLLLSG
                     TTCYAALKRFAEMFEQWGGVFVHSTYMVFAGGGFPPGFAYDLARPIESLAEKQLLSAW
                     RGFSMPMFYAQEWLAETVREWSVDGICFHGVKSCRTTSTGLPDVREWLRTHFDIPGLF
                     IQSDLVDPRLWSDAQLKNRVDAFIESLASRRATAGR"
     gene            4007..4819
                     /locus_tag="VEW91_07025"
     CDS             4007..4819
                     /locus_tag="VEW91_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HYM91370.1"
                     /translation="MLVAGVDVGSTQTKAIVMNDTRIIGRALLDTGARLTEAAAEAFR
                     QALGTAGASEADVACVIGTGYGRFRVQFGHAQVTEISCHARGAVYLFPRTRSVLDIGG
                     QDTKAISVSESGQVLDFCMNDKCSAGTGRFLGAASQALEIPLGELGPLAQTSKNPVTM
                     TTTCTVFAESEILGWLARGRKTEDVLMGVHVAIASRSLSLLRRVGVEPEITFTGGVAR
                     NVAMVRLLGELTGVPVNVSEESHYCGAIGAALFALDRVLVGAQAGAGGGRDA"
     gene            4816..5658
                     /locus_tag="VEW91_07030"
     CDS             4816..5658
                     /locus_tag="VEW91_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HYM91371.1"
                     /translation="MSLTIGMDMGSTYTKAVIADPEGQVLGKVTFHSGFNFAKAAEQG
                     LQLLLETAGVPREHVDYIAATGYGRYMVPFRDLAITELTCHARGLFWMFPGVRTVLDI
                     GGQTVKAIRLDDRGRVKAFRLNDKCAAGSGAFLEKTMRYLGYQPADIVPLSGAASDPA
                     TISSVCAVFAESEVINHLTAGRSPEDVCAGAVIALAERAGQVFKRVKPEPEYALSGGL
                     TRVPLMRRVLESSLKCTFHVAPDDDGVYAGAIGAALLAHDRLRKLAEQKPLEGAGATR
                     GREN"
     gene            5660..6637
                     /locus_tag="VEW91_07035"
     CDS             5660..6637
                     /locus_tag="VEW91_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYM91372.1"
                     /translation="MGTIREALDASLRGRSPDSFHFGDIVYQKCDWVARVTINRPNVY
                     NAYRTQPLAEMRDAFRDAAVDDGVAVLVLTGAGEKAFCTGGDVTEYATKYTQRPHDYW
                     KYMSLFIECHDALRNVGKPTIARLNGIVAGGGNEFNMACDLAVAADDITFRQVGTRVG
                     SVAAGGATQWLPLLVGDRRAREMLLLSEPITARQAQEWGLVNRAVPRGELDATVNDFC
                     QKLIDKFPECLRYTKQQVNFWKDLAWTLTAGHARDWLSLHFACREPWEGMTAFVEKRP
                     PDYRGLREMARDGGSPEYLWGPPANTCPSCGVRGLPAGMAYCGKCGHRL"
     gene            6661..7416
                     /locus_tag="VEW91_07040"
     CDS             6661..7416
                     /locus_tag="VEW91_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM91373.1"
                     /translation="MISRDSVVLVTGGASGIGKAACLAFAAGGARVAVADVNSEGAEK
                     VAGAALGAGGDGLPVTMDVTSRASVAGGIQSVLAHWGRIDILVNVAGWDRITPFVDTT
                     EEFWDKVIAINFRGILATCHAVLPHMIERKRGVVVNVASEAGRSGSSGEAVYSGTKGA
                     VIAFSKAIAREVARYGIRVNVVAPGLTDTPLLQALIAEGNEKLISAIVKATPLQRLAR
                     PEEIADAVLFLASEKASFLTGQTVSVGGGLTMI"
     gene            7472..8029
                     /locus_tag="VEW91_07045"
     CDS             7472..8029
                     /locus_tag="VEW91_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HYM91374.1"
                     /translation="MRSMQQLAADHDHILEVLRTLDRVLHDADRTRTVPSEFLRTLVT
                     FSQSFVDLCHHGKEEGCLFPCLERCGMPREGGPIGIMLQEHEKGRALVREIADRLERY
                     EAGRARVDEVLAPCREYVDLLQHHIEKENTILFPMGEHCMGDDDDDATVTCFEEREAE
                     LGPGARERLVQLGQALSAATSEPAS"
     gene            8208..9149
                     /locus_tag="VEW91_07050"
     CDS             8208..9149
                     /locus_tag="VEW91_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM91375.1"
                     /translation="MALPVPFPLKTANIYLVAAPEGTVLVDTAFYTPQAWEHLTRAVT
                     EFTAIAGPLRAIVLTHHHPDHVGMAGRLQHHYGVPVLASAREAELIAEVWDPRTLSEG
                     TGFYWEHGMPASLLESIIEEHRLLFVSLAPLPDVEPVPMDGALALAGLRLTGVITPGH
                     SPAHLCLFHAASGALIVGDHLLPRITPNIGWYPYTSPDPLRDFLASLDVVERLSVRRA
                     FPGHGPVIVDIVSRIAELRTHHADRLTATRALLDGNALTGYQVALGLFGGGLSSQEAR
                     LAMVEALAHLEFLRQQGEVALERCGTQVVYRQHVQRR"
     gene            9204..>9966
                     /locus_tag="VEW91_07055"
     CDS             9204..>9966
                     /locus_tag="VEW91_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HYM91376.1"
                     /translation="MSVTLHQALRSGLLAASGNLALASWVRRHGALLGARRFVAGETL
                     DECVRVLRRLRDEGFSTTTTLLGEAVTSEQAARGAAAEYAAVLRRLADEDTRTTVALK
                     LTHLGLDVSEALAYETVSHVVRCAAALGNFVRIDMEQSSKVDPTLRLYRRLRSEGLTN
                     VGVVLQACLYRSDADLRALLPLRPNVRLVKGAYLEPREIAFPRKRDVDRSFVRLIALA
                     LSGEGYTAVATHDERVIERTLEVARADGIDFDRVEF"
BASE COUNT         1623 a         3275 c         3457 g         1611 t
ORIGIN      
        1 acccgtgacc taccgtgaca aggccgcgtc gccgattttc gcatcaaaat agagcatttg
       61 ccccaggcaa atgaggctga caccccatgc caatcggccg atgcagtcct accataaatt
      121 cgagcgagcg ttcgatcggg aattccatga catacgatgc gcggcgggag acgatcctca
      181 gggcggccgg ggcgttgttt gcctcaaagg gatacgagcg gacgtccatc cgggatgtcg
      241 cccgcgcctc gggcatgagc gtggccggcc tgtactacta cgtgcgcagc aaagaagagc
      301 tcctctacca catctgcgac cgcagctttt ccacgatcct cgaggccctc gaccgcgggc
      361 ttgcccatac cacggacccc cgggagcgcg tgcgggtgct gatccgtaac catctgtcgc
      421 actttcttcg tgacatgaac gcgatcaagg tcatcacgcg cgaactcggc gccctggacg
      481 gcgtccatgc caccaggatc gcggccctcc gccgacggta ctacgcgacc tgtcgcgccg
      541 tgctcgaggg gctggggacg catccggtgg ccgcgcgact cgcggcgatg agcctgtttg
      601 gcatgatcaa ctggattcac atgtggtacc gacccgaggt ggacggcgac gccgaccatc
      661 tcgccgatgg catggcgcgg atcttcctcg atgggtacgg cacaccgtca ggaggaacga
      721 ccacatggtc gcggccgatg gccacgtcac gctgacccga gacggccggg cggcccgcat
      781 cgtgttggat cgcccgcccc tcaacgtcct gacgcttgag atgatccagg agctcaccgc
      841 gatctgcgat cgtttggccg aggccccgga gatcgtgatc gtgacgctcg cggcggccgg
      901 cggccgcgcg ttttgtgccg gcgtcgacgt ggccgcccac gcgcccgacc gcgcggaacc
      961 catgctggcg gctttcgagg cgatggcgga gcgtctcctg gcgctcgacc cgattctcat
     1021 cgcggccgtg tccggcgcgg cgctcggcgg agggtgggag ctcgcgctgt tgtgcgactt
     1081 cgtcgtcgcc tcggaacgcg cgatgttcgg cgtccccgag gtcaagctcg ccgcgcttcc
     1141 tccggtggcg gcggccatcc tgcctctcct ggtcggccac ctccgcgcgc tcgagttcgt
     1201 ggtcacgggc cgtcagatct ctgctcatga ggcccagggg ttggggctcg tgagccgcgt
     1261 cgcctcggca gagaatgtgg aaacggcaac tcgggagctg gtggcgacgc tcctgtctca
     1321 gagcgggccc gcgatccgcg cggcgaaggc cgcggtcgtg gccccgcgtc gcgatgcgat
     1381 ctgccgcagc cttcgcgcgg ccaccgaggt gtaccgttct cgcttgcttc ccacccagga
     1441 tgcggcagaa ggcatcgcgg cgttcctgga aaagcgcgcg ccggcgtgga agcatgtctg
     1501 accgcgaggc gtcctggtga aggccgagca ggaactgggg gaactcattg agcggtgccg
     1561 cacgctggcc gaggatccgg ggtatccggc ggtccgccgg tggctggagg ccaatccttg
     1621 cgggaaggtg ctggggcact tccaggtcta cttccccgag gaaatcgcgc acgcggcggg
     1681 gatgctgccg gtcaagatcc tcggcgccgg cagcgcgatc cagatccgca aggccgacgc
     1741 gcggatcgcc gcgttcgtct gctccatcat ccgcagctcc ctggaactcg ccctcaccga
     1801 ccggctgaac tttctgtcgg tgttcgtcac cccgccgatc tgtgatgccg cgcggaatgc
     1861 ctgcgcggtg tgggtgcgca acctgccgaa catcaactgc caggtcctgt acctgccgca
     1921 gaacgccgcc tcaccgcacg cggcgtcctt tctccggggc gaataccggc gcatcgccgg
     1981 cgtcatcgag gagacaaccg ggcagccgat cacggacgag gccatgcgcc agagtatcgc
     2041 cctcttcaac gagaaccggc gattgctgcg cgagctgtac cgggtgaagc gggagacgcc
     2101 gtggctcctg tctgccgtgg aggcgtacac gctcgtgcgg gccggtggcc tcatgccacg
     2161 cgaggaacac aaccccctgc tgcgccgtgt gctggacctg gtgccgcagc ggcaggcgaa
     2221 gcgccaggac aggatccggg tggtcttcga aggcggctac tgcgagcagc ctcccgtcga
     2281 catgctggcg gtcattcagg acgcctgcta catcgtggac gatgatctgc tcatcgggat
     2341 gcgctggctc accggcgacg tccccaccga aggggaaccg ctggccagcc tggcccgagc
     2401 gtacctcgac gggtcgagct acagcccggt gcagcacgac ccccgcaagc ccaagccgga
     2461 gatgctgctc cagcgcatcc gggagtcaca cgccgacgcc gcgatcgtcg ccgcgcccaa
     2521 gatgtgcgag ccggggttgg aagagcaggt caaccacatc cgcgcgctgg aacaggcggg
     2581 gatcccgcac ctggtgatgg agttcgagga gaagatgacc gtcttcgagc agatgcggat
     2641 ggaagtcgag acgttcgccg agtcgctgtt gttattcgcg taagcccgtc tccggacggg
     2701 aggagggctc cacgatgacg accgagacac cgacgcggat cggatcgatc cgggacgagg
     2761 gccggcgtct gctggagcgg tggtggcagg agatgggggc cgccgaccag gcggggcgtc
     2821 ccacggcgta cgtctttgtg atggggtccc tcgacgagat cctcagggtc tttgacctgc
     2881 cgatcaactg ccccgagatc acttcgctcc agaccgccgt ccggggccag tccctgcagt
     2941 acctgcaggc cgcggaggac tatggctact cccccgacat ctgcggctat gtgaagacgg
     3001 acgtgggcct gcagctcaag gacaggcagc atccgaacgg caaggtcccg aagcccgccc
     3061 tggcgatcgc cacgaacatg tgcaacacgt acatcaagtg ggccgagatc tgggagcaga
     3121 tgtaccactg ccccatcttc gtcctcgacc tccccgcgtg gcgagggacg ggagccgcgc
     3181 ccggcgttga tagcgagacg ttccagaacg accgccgcta cgtggagggg cagctgcggg
     3241 acctgatcgc cctctgcgag cgcctgaccg ggaaggcctt cgacatcgac cggctgcgcg
     3301 aggtcatggg cgaggtgaac cgcatggccg cgtcctacca cgaggtgctg gcgctcaaca
     3361 agcaccggcc ggcgccgttc agcgccatcc gcgaggggat cacgttccag ggcatctcca
     3421 acctctaccg gggctcgccc gaagggtccc gcttcttcgg cgtcgcccgg gaggagatcc
     3481 aggagcggat ccgccggggg atgggaacgc tcgaggagaa gtaccggctc ctcttgagcg
     3541 gcaccacctg ctacgccgcc ctcaaacggt tcgcggagat gttcgagcag tggggcgggg
     3601 tcttcgtcca cagcacgtat atggtgtttg ccggaggagg ctttcccccc gggttcgcct
     3661 acgacctcgc tcgcccgatc gagagcctgg ccgagaaaca gctgctctct gcctggcggg
     3721 gattcagcat gccgatgttt tacgcccagg agtggctggc agagacggtt cgggagtggt
     3781 ccgtcgacgg gatctgcttc cacggcgtga agtcgtgccg tacgacctcc accggcctgc
     3841 ccgacgtgcg cgagtggctg aggacccact ttgacatccc gggcttattc atccagtccg
     3901 atctcgtgga cccgcggctg tggtcggatg cgcagctcaa gaaccgggtc gacgccttca
     3961 tcgagtcgct ggccagccgc cgggcaacgg cggggaggtg acggggatgc tggtcgccgg
     4021 ggtcgacgtg ggatcgacgc aaacgaaagc catcgtgatg aacgacaccc ggatcatcgg
     4081 ccgggcgctc ctcgacaccg gtgcccggct gaccgaggcg gccgccgagg cgttccgcca
     4141 ggccctgggg acggcggggg ccagcgaagc ggatgtggcg tgcgtcatcg ggaccggcta
     4201 cggccggttc cgcgtccagt tcggtcacgc ccaggtgacc gagatctcct gccacgcccg
     4261 cggcgccgtc tacctctttc cacgcactcg cagtgtgctg gacatcggcg gccaggatac
     4321 gaaggcgatc agcgtgagcg agagcgggca ggtgctcgac ttctgcatga acgacaagtg
     4381 ctcggcgggg accgggcgct tcctgggcgc cgcgtcacag gcgctggaaa ttccgcttgg
     4441 agaactcggc ccgctcgcgc agacgtcgaa aaaccccgtc acgatgacca cgacctgcac
     4501 cgtctttgcc gagtcggaga tcctgggctg gctggcccgg ggccggaaga ccgaggacgt
     4561 gctgatgggg gtgcacgtgg ccatcgcctc tcgaagcctc tccctgctgc ggcgcgtcgg
     4621 cgtggagccg gagatcacct tcacgggtgg ggtggcccga aacgtcgcga tggtccggct
     4681 gctcggggag ctcaccggcg tccccgtcaa cgtcagcgag gaatcccact actgcggcgc
     4741 gatcggtgcc gcgctcttcg cgctggaccg cgtgctggtg ggcgcacagg ccggcgccgg
     4801 aggggggcgc gacgcatgag cctgaccatc gggatggaca tggggtccac ctacacgaag
     4861 gccgtcattg ccgaccccga ggggcaggtg ctcggcaagg tgaccttcca cagcggcttc
     4921 aactttgcga aagcggccga gcagggactc cagctgctcc tcgagacggc cggcgtgccg
     4981 cgcgagcacg tggactacat cgcggcgaca ggctacgggc ggtatatggt ccccttccgc
     5041 gacctggcga tcaccgaatt gacctgccac gcccgggggc tcttctggat gttccccggc
     5101 gtccggacgg tgctggacat cggcggacag accgtgaagg ccatccgcct ggacgaccgc
     5161 ggccgggtca aggccttccg cctgaacgat aagtgcgccg cgggcagcgg ggcgtttctc
     5221 gaaaagacga tgcgctacct gggctaccag cccgcggaca tcgtccccct ctccggtgct
     5281 gcgagcgacc cggcgacgat ctcgagtgtc tgcgcggtct tcgccgaatc ggaggtgatc
     5341 aaccacctga cggccgggcg cagccccgag gacgtgtgcg cgggggccgt gatcgcgctg
     5401 gccgagcggg ccgggcaggt gttcaaacgc gtgaaacccg agccggagta tgcgctgagc
     5461 ggcggcctca cccgtgtgcc gctcatgcgc cgggtgctcg agtcctcgct caagtgtacg
     5521 ttccacgtgg ctcccgacga cgacggggtg tacgcggggg ccatcggggc ggcattgctg
     5581 gcccacgacc ggctccgcaa gctggcggag caaaagccgc tcgagggcgc gggggccacg
     5641 cgaggaaggg agaactgaga tgggtacgat tcgggaagca ctcgacgcgt cgctgcgcgg
     5701 tcgttccccg gacagcttcc acttcgggga catcgtgtac cagaaatgcg actgggtggc
     5761 gcgcgtcacg atcaaccgcc cgaacgtcta caacgcctat cggacccagc ccctcgcgga
     5821 gatgcgcgac gccttccgcg acgcggcggt cgacgacggc gtggcggtgc tcgtcctcac
     5881 cggcgccggc gagaaggcgt tctgcacggg cggcgacgtc accgaatacg ccaccaagta
     5941 cacgcaacgc ccgcacgact actggaagta catgagcctg ttcatcgaat gccacgacgc
     6001 actgcgcaac gtcggcaagc cgacgatcgc ccgcctgaac ggcatcgtgg ccggaggggg
     6061 aaacgagttc aacatggcct gcgaccttgc cgtcgccgcc gacgacatca ccttccgcca
     6121 ggtcgggacg cgggtcggca gcgtcgccgc cggaggcgcg acccagtggc tgccgctcct
     6181 cgtcggggac cggcgggcgc gtgagatgct gctcctctcc gagcccatta cggctcggca
     6241 ggctcaggag tggggactgg tgaaccgcgc ggtgccgcgg ggggaactcg atgccacggt
     6301 caacgacttc tgccagaagc tcatcgacaa attcccggaa tgcctgcggt acaccaagca
     6361 gcaggtgaat ttctggaagg atctggcctg gaccctcacc gccggccacg cccgcgactg
     6421 gctgagcctc catttcgcct gccgcgagcc ctgggaaggg atgaccgcgt tcgtcgaaaa
     6481 gcggcctccc gattaccgcg ggctgcgaga gatggcccgc gacggtggct cccccgagta
     6541 cctctggggt ccgccggcca acacctgtcc gtcctgcggg gtccgtgggc tgcccgccgg
     6601 tatggcctac tgcggcaagt gcgggcaccg gctctagcaa tggcagagga ggggaatcgc
     6661 gtgatttctc gcgactcggt cgtcctcgtc accggcggcg ccagcggaat cgggaaggcc
     6721 gcgtgcctgg cctttgccgc cggaggggct cgcgtggcgg tggccgatgt caacagtgaa
     6781 ggggctgaga aggtggccgg cgcggcgctc ggcgcaggcg gggacggcct gccggtcacc
     6841 atggacgtca ccagccgggc gagcgtggcc ggcggtatcc agagcgtgct cgcccactgg
     6901 ggccgcatcg acatcctggt caacgtggcg gggtgggacc gcatcacgcc gtttgtcgat
     6961 acgacggaag agttttggga caaagtgatc gcgatcaact ttcgcggcat cctggcgacg
     7021 tgccacgcgg tgctgccgca catgatcgag cgtaagaggg gggtcgtcgt caacgtggcc
     7081 tccgaggcgg gccggtccgg atcctcaggc gaagcggtgt actccggtac gaagggagcc
     7141 gtgatcgcct tcagcaaggc gatcgcccgc gaggtggcgc ggtacgggat ccgggtcaac
     7201 gtggtcgcgc ccgggctgac cgacaccccg ctcctgcagg ctttgatcgc cgagggcaac
     7261 gagaagctca tctccgcgat cgtcaaggcc actcccctcc agcggctcgc gaggcctgag
     7321 gagatcgccg atgccgtgct gttcctagcg tcggagaagg cgtcattcct taccggccag
     7381 accgtgagcg tcggaggggg gcttacgatg atctaggcgt gtcggcgacg cacgtcgaat
     7441 cgcggaagga acgggcagac ggagccgagt catgcggtcc atgcaacaac tggcggcaga
     7501 tcacgatcac atcctggagg tccttcgcac gctggaccgt gtactacacg atgccgaccg
     7561 aacccgtaca gtcccgtccg aattcctccg gaccctggtc acgttcagcc aatccttcgt
     7621 ggacctctgc caccacggca aagaggaggg gtgcctgttc ccctgcctgg agaggtgcgg
     7681 gatgcccaga gaggggggcc ccatcggcat catgctccag gagcacgaga aagggcgggc
     7741 gctggtaagg gagatcgccg atcggttgga gcggtatgag gcgggccggg cgcgggtcga
     7801 tgaggtgctc gccccctgcc gtgagtatgt ggatttgctg cagcaccata tcgaaaagga
     7861 gaacacgatc ctgttcccga tgggggagca ctgcatgggc gacgatgatg acgacgcgac
     7921 ggtgacgtgc ttcgaggagc gggaggcgga actggggccg ggcgcgcgcg agcggctcgt
     7981 gcagcttggg caggcactct ccgccgcgac gagcgagccc gcatcatgag gcggtccgtc
     8041 ctgcgaggag aggcccggat ggtgaacctc ggagggttta ggatcacgcg cgggacccac
     8101 gcaggggtgt gagcgtgccg ctgagccgca ggtcagatca cccctgcgct ggcgatcctc
     8161 ctcgctccgg cccggcgccc ggggaagcgt tcccccgggt tcatcaagtg gcgttgccgg
     8221 tcccgttccc cttgaagacg gccaacatct acttggtcgc cgccccggag gggaccgtat
     8281 tggtcgacac agccttctac accccccagg cgtgggagca ccttacccgc gcggtgacgg
     8341 agttcaccgc catcgcgggg ccgctccggg cgatcgtgct gacccatcac catccggacc
     8401 atgtcggaat ggcgggccgc cttcagcacc attatggcgt cccggtgctg gcctcggcga
     8461 gagaggccga gctcatcgcg gaggtgtggg atcccaggac cctgagcgag gggacgggat
     8521 tctactggga gcacgggatg ccggcgtccc tgctggagag catcatagag gagcatcgcc
     8581 tcctgtttgt gtcgctcgcc cccctgccgg acgtcgagcc agtccccatg gatggggccc
     8641 tcgcgctggc cggcctccgc ctcactgggg tgatcacccc gggccactcg cctgcgcacc
     8701 tctgcctctt tcacgcggcg tccggggcct tgatcgtcgg tgatcatctg ttaccgcgga
     8761 tcactccgaa catcggatgg tacccctaca cgtccccgga tccgcttcgc gatttcctgg
     8821 catcgctcga cgtggtcgag cgattgtcgg tgcggcgggc ctttcccggg catgggccgg
     8881 tgattgtgga catcgtgagc cgcatcgccg agttacgcac ccatcatgcg gatcgcctca
     8941 cggccacccg tgcccttctc gatggaaacg cgctgaccgg gtaccaggtc gccctcggcc
     9001 tcttcggcgg cggcctatcc tctcaggagg cgcggctcgc gatggtcgaa gcgctggccc
     9061 acctagaatt cctgcgccag cagggcgagg tggctctcga acggtgcggg actcaggttg
     9121 tctatcgcca gcacgtgcag aggcgatgag acgatgctgg tagaccgagc cgtccgggaa
     9181 cggtggctcg cgggaggata gcgatgtcag tcacgcttca ccaagcactg cgcagcggtc
     9241 tgctggccgc ctccggaaat ctcgcactgg ccagctgggt gcgccggcat ggggcgttgc
     9301 tcggcgccag gcggttcgtc gcaggggaga cccttgatga gtgcgtccgc gtcttgcgcc
     9361 gcctccgcga cgaaggtttc agcaccacga cgacgttgct gggcgaggct gtcacgagcg
     9421 agcaggccgc gcgaggcgcg gccgcggaat acgccgcggt gctcagacgc ctcgcagacg
     9481 aggataccag gacgacggtg gccctcaagc tgacgcatct cgggctggac gtgagcgaag
     9541 cgctcgccta tgagaccgtc tcccacgtcg tgcggtgcgc cgccgcgctc ggcaacttcg
     9601 ttcgcatcga tatggaacag tcgtcgaagg tcgatccgac gctgcggctc tataggcgtc
     9661 tcaggtcgga ggggctgacg aacgtcggcg tggtgctcca ggcatgcctg taccgcagcg
     9721 acgccgacct ccgggcgttg ctcccacttc gtccgaacgt gcgcctcgtg aaaggcgcat
     9781 acttggagcc tcgggagatc gccttcccgc ggaagcgcga cgtggatcgc agctttgtcc
     9841 ggctgatcgc gctcgcgctt tccggcgagg ggtacaccgc cgtggcgacg cacgacgagc
     9901 gcgtcatcga gcggacgctc gaggtcgctc gcgcggacgg gatcgacttc gatcgcgtcg
     9961 aattcc
//
LOCUS       DASQGH010000361         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7319, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000361 DASQGH010000000
VERSION     DASQGH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7319"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VEW91_07060"
     CDS             <1..334
                     /locus_tag="VEW91_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM91377.1"
                     /translation="QIIQERLVNMLTEITTAQLLAYHLGRLKDAGTMRHTQVSMAKRN
                     NVRMALRVAREARAILGGYGITLEYHAMRHAANLESVDTYEGTYDVHTLILGRDITGF
                     DAFGAPQA"
     gene            361..1722
                     /locus_tag="VEW91_07065"
     CDS             361..1722
                     /locus_tag="VEW91_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM91378.1"
                     /translation="MDRSADAPGAAPGIFGRITLHGRITLIAGLAFASNGLNLGILSF
                     ALLGLKASWGLTPAQVGVVTGAAGLGQLIGGVLVGHATDRIGRRRGYGLTIALNSLAT
                     GAAALAPSFGWLVPLILLAGIGFGGVAPVAISLVGEFAPREKRGALIGWTQVMWVLGW
                     IAAATGGVLLLHGVGWRGILGIGILPIVLAVIGPSLVPESPRFLLAHGRRRDAEDLVR
                     TLEARYGVSLELPAQERARRESVLAHLRELWSPRFLRRTALLWTAWFVMIGTYQGPVV
                     WFPTLLAAAGVPHAAEASLVMAWAMLPTTVAATLLLDRLGRKPVMVSALALASVAAAG
                     AALARTGPGFVVSAAGLAGGVLAAWVPVLTYAAELYPTRIRATATGWATGAGRSAGIL
                     APAMLGLLMTTWGGRQGTVFGIFAAALGIAALIVLLLGEETAGRSLEEIAGTRTASAA
                     ASP"
BASE COUNT          252 a          594 c          644 g          322 t
ORIGIN      
        1 ccagatcatc caggagcggc tcgtcaacat gctcaccgag atcaccacgg cccaactgct
       61 cgcctaccat ctggggcggc tcaaggacgc cgggacgatg cggcacaccc aggtcagcat
      121 ggccaagcgg aacaacgtca ggatggcgct gcgcgtggct cgggaggcac gcgccatcct
      181 tggcggatat gggatcaccc tcgagtacca cgccatgcgg cacgcggcca acctcgagtc
      241 ggtggacacc tacgagggca cgtacgacgt ccacacgctc atcctggggc gcgacatcac
      301 cgggtttgac gcgtttggcg caccccaggc gtgagctggg cgggttccgg accacgcggg
      361 atggaccgat ctgcggatgc acccggcgcc gcccccggga tctttggccg gataacactc
      421 catgggagga tcacccttat cgccgggctg gcatttgcca gtaacggcct caacctcggg
      481 attctcagtt ttgctctcct cggtctgaag gcctcgtggg ggctcacccc cgcccaggtc
      541 ggcgtggtga cgggggcggc cggactgggg caactcattg gtggtgtcct tgtgggccac
      601 gccacggacc ggatcggccg tcgacgagga tacgggctga cgattgcact caacagcctc
      661 gctaccggag ccgcggcgct cgctccgtca ttcgggtggc tcgtgccgct cattttgttg
      721 gcgggcatcg ggtttggggg agtcgcgccg gtggcgatca gcctggtggg cgaatttgcc
      781 ccgcgcgaga agcggggtgc gctcatcggc tggacccagg tgatgtgggt cctggggtgg
      841 atcgcggctg cgacgggagg agtcctgctg ctccatgggg tcgggtggcg gggcatactt
      901 ggcatcggga tcctcccgat tgtgctcgcc gtcatcggcc cctcgttggt gcccgagtcc
      961 ccacgttttc tactcgccca tggccggcgg cgcgacgccg aggacctggt ccgcaccctc
     1021 gaggcgcgct atggggtgag cctggaactg ccggcgcagg agcgagcccg ccgggagtcg
     1081 gtccttgcgc acctgcgtga actctggagc ccgcgcttcc tgcgccgaac cgctcttcta
     1141 tggacggcgt ggttcgtcat gatcggcacg taccagggtc cggtggtgtg gttcccaacg
     1201 ctgctggccg ccgctggggt cccgcacgca gcggaagctt cccttgtgat ggcctgggcg
     1261 atgctcccga ccacggttgc cgccacactg ctcctcgacc gcctgggcag gaagccggtg
     1321 atggtcagcg cgttggccct ggcctcggta gccgcggcgg gggctgcact ggcgcggacc
     1381 gggcccggtt ttgttgtgag cgcggccggc ctcgcggggg gcgtcctggc ggcatgggtc
     1441 cccgtgttga cctacgccgc cgagttgtac cccacccgca tccgggccac cgcgaccggc
     1501 tgggcgacag gtgccggccg ctcggctgga attctcgccc cggcgatgct cggtctgctc
     1561 atgaccacct ggggtgggag gcagggcacg gtgttcggca tctttgcagc ggcgctcggc
     1621 atcgccgcgc tcatcgtgct gttgttggga gaggagacgg ccggtcgatc actcgaagag
     1681 atcgcgggca cccgaacagc gtccgccgcc gcatcgccgt aggatccgac agtggtgatg
     1741 caccctcgaa gtggagccca cggtccggag gcgtgacgat gtctgtaagc gtccgcattc
     1801 tcacggctga tg
//
LOCUS       DASQGH010000362         6874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32800, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000362 DASQGH010000000
VERSION     DASQGH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32800"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VEW91_07070"
     CDS             <1..462
                     /locus_tag="VEW91_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91379.1"
                     /translation="LPFATEASFDFSFSGLKTAVVRALAAASTGTAGERDAGFAADLA
                     AAFQENLVEVLVAKTARAAGERAARAVILAGGVAANALLRSRMAAATARLGVPFYVPP
                     IALCTDNAGMIAAAGWFRLSRGLRTSLRLGAYADLDLGGEVPETTQGPPMG"
     gene            692..1003
                     /gene="groES"
                     /locus_tag="VEW91_07075"
     CDS             692..1003
                     /gene="groES"
                     /locus_tag="VEW91_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HYM91380.1"
                     /translation="MNLKPLGDRIVVRVIEELERTKGGIVLPDTAKEKPQEAEVLAVG
                     PGGRNEKGDRVPMEVKIGDRVIFAKYSGTEFKRDDEEYLILRESDVLAIVTREKVKAR
                     A"
     gene            1017..2627
                     /gene="groL"
                     /locus_tag="VEW91_07080"
     CDS             1017..2627
                     /gene="groL"
                     /locus_tag="VEW91_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275112.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYM91381.1"
                     /translation="MPKMLLYHEEARRALERGVNRLADVVKITLGPKGRNVVLEKKFG
                     SPTITHDGVTVAKEIELEDPFENAGAQLVREVATKTNDVAGDGTTTATVLAQAMIKEG
                     LKNVAAGANPMVVKRGIDRAVESVVEVLRSQAKPVETKAAIAQVASISAHEEAIGQLI
                     SDAMDKVGKDGVITVEESKGIETTVETVEGMQFDKGYISPYMVTDPEKMEAVLDDPYL
                     LITDKKVSAVKDLLPALERIVQVSKPLLLIAEDVEGEALATLVVNKLRGTLQAVAVKA
                     PAFGDRRKAMLQDIAILTGGQVITEELGLKLDNVELGMLGKARQVKIGKEETIIIGGA
                     GKQSEIQKRISELRKQIDETESDYDREKLQERLGKMVGGVGVIKVGAASETELKEKKH
                     RVEDALSTARAAVEEGIVPGGGVALIAAISALEKVDATGDEKVGVDIVRRALEEPTRQ
                     LARNAGAEGSIIVEKVRASKAGWGFNVLSGEFQDMFKAGIVDPCKVTRSALQNAASVA
                     SMILTAEVLVVDKPEEEKEAPMPPTPPM"
     gene            2683..4143
                     /locus_tag="VEW91_07085"
     CDS             2683..4143
                     /locus_tag="VEW91_07085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM91382.1"
                     /translation="MTAEARRGEVSQASPRRIWPIAALLLLALVRGAIASRTPIIDDE
                     GYYWLWSRHLSLSYLDHPPLVAWLDALATSAGRTEWLLRLPAMVATLGTTLVLYGLGG
                     ELFGPRAGLRAAALHAAVPVFALQAIHAIPDPLLYLWWAAAMWAFWRAVHGEPRMWWV
                     CGLALGLGALSKYTVLILLIAMLGMLRWPRYRRWWRTPGPYLAGALMCAVALPVLVWN
                     AQHGWASLRFWLNAGGMQGPPPGTGKGWGLPSVAAVQFAYGGPLLFPAMVWALWRSWR
                     MARDDERFAFLAMAGGPVLLLVAGSSLLGVDRPNWPAPAYLAGALALGALWPRLLAPA
                     AFASSLALSLAVSVLITLIPQLPPALQWSELYGWREVTQEMLRRADALRDPGHVLLLG
                     RRYNEASYFGYYTMDRVPVSTGHLSALSFWAPPHRFVGWQGIAAIDPASGVDELAGAC
                     ARLVEQPPYFVRFDGGGGRRFRLFHCIGYRGTPRPP"
     gene            4188..6209
                     /locus_tag="VEW91_07090"
     CDS             4188..6209
                     /locus_tag="VEW91_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYM91383.1"
                     /translation="MLDRPLVSFGREVCGDLSAGLRREWLVTNGLGGYASGTVAGVNT
                     RRYHGLLVAALTPPVGRTVLVGGLVEWARYDGRRYPLATHEYGDGTVDPRGYRHLQSF
                     ALEGMLPVWVFALGDALLEKRVWMAHGANTTYVMYRLVRATRAVELEITPLVTYRDFH
                     TLTSGRGWRPEAEAAPHGVVLRAFPGAVPFRLLLEGGRFVARGDWYWNFLHREESARG
                     LDDRADLYAPGAFTAALTPGESLTLVGTADATPPAGGPRSLAAVQARQQHLLRRAGVT
                     DSNPVVQQLVLAADQFIVRRTTGGRGATADAGQTVIAGYHWFNDWGRDTMIALPGLTL
                     ATGRPEVAAEILRTFTRYLADGLLPNNFPDAAGAGPGYNTADASLWYVLAVRAYHEAT
                     GDDQLVDEVLPALREVIDRHERGTRYGIGMDPADGLLRAGAPGVQLTWMDAKVGDWAV
                     TPRAGKPVEINALWYNALRTLAAFLASRRDPAAPRYRALADRVRAAFRARFASQGRGS
                     LADVVDGPGGDDLAVRPNQIFAVSLPFPLLERAAAAAVVDEVGRALLTTYGLRSLSPD
                     DPAYHGAYGGDPPHRDRAYHQGPAWAWLAGAYAEAYHRVHGDLEGARALLRPFEHHLR
                     DAGLGTISELFEGDPPHLPRGCIAQAWSVGEVLRVWRVLEKGRPRRGAW"
     gene            6242..>6874
                     /locus_tag="VEW91_07095"
     CDS             6242..>6874
                     /locus_tag="VEW91_07095"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HYM91384.1"
                     /translation="MIIYDLRRRPMSKLRDFTLLSPRAQLDRSFYDRLRAARPQYRLA
                     ERFVIPPFEGRGFIATKGQVFRVIEEEGVQIGDICFWNAHNAKEMYSLSRTWAIEGWA
                     VRPYTRIWSDVPWLRPMATCLDDTVVPASADAGFHHHWLGTHCATEWTEMRVRIPGLN
                     SCHLNFLQAITPFGLTEEDIRDNVNLFQKMRPDPETGRISAALSDSKKGDY"
BASE COUNT         1067 a         2254 c         2491 g         1062 t
ORIGIN      
        1 ctcccgtttg cgaccgaggc gagcttcgac ttttcgttca gcggactgaa gaccgcggtc
       61 gtccgcgccc tggccgccgc atccacgggg acggcgggcg agcgggatgc ggggtttgcg
      121 gcggatcttg cggcggcgtt ccaggagaac ctcgtcgagg tgctcgtcgc gaagacggcc
      181 cgcgcggccg gggagcgggc ggcccgggcg gtcatcctcg caggcggcgt ggcggccaac
      241 gcgctcctca ggtcgcggat ggccgccgcc accgcccgcc tgggggtccc gttctatgtc
      301 cccccgatcg cgctgtgcac cgacaacgcc ggcatgatcg ccgcggcggg gtggttccgg
      361 ctctcgcggg gcctaaggac ctcgctccgg ctcggcgcct acgcggacct ggacctcgga
      421 ggcgaggttc cggagacgac ccagggtccg ccgatgggct gaggaggccc cgcgcggccg
      481 ggagggcctc ctcggggtgg aggcgaacga gcgtttctat catgcaaact tgaaaatctc
      541 gaagatctgc cccgtccggg cgagattaca ggggggtacc tccgattttc cgcccaattt
      601 ggggttgcct gggcgatggg acttgcctat attgagtatg gattagcact ccatcatcgt
      661 gagtgctaag actcttgccg aggaggacgg tatgaatctg aagcctctgg gggatcggat
      721 cgtggtcagg gtgatcgaag agctcgagcg caccaagggc ggcatcgtgc tgcccgacac
      781 cgccaaggag aagccgcagg aagccgaggt cctcgccgtc ggcccgggcg gccggaacga
      841 aaagggagat cgggtgccga tggaggtcaa gatcggggac cgggtgatct tcgccaagta
      901 ctcgggcacc gagttcaagc gggatgatga ggagtacctg atcttgcgcg agagcgacgt
      961 gctcgcgatc gtgacacgcg aaaaggtgaa ggcgagagcc taggggggac accgggatgc
     1021 ccaagatgct cttgtatcat gaggaggcgc ggcgcgccct cgagcggggc gtcaaccgcc
     1081 tggccgatgt cgtgaagatc acgctcggcc cgaaggggcg caacgtcgtc cttgaaaaga
     1141 agttcgggtc gccgacgatc acccacgacg gggtgactgt ggccaaggag atcgagctgg
     1201 aggacccgtt cgagaacgcc ggggcccaac tggtccgcga ggtcgcgacc aagaccaacg
     1261 atgtcgcggg cgacgggacc acgacggcga cggtgttggc ccaggcaatg atcaaggagg
     1321 gtctcaagaa cgtcgcggcc ggggccaacc cgatggtggt gaagcgcggg atcgaccggg
     1381 cggtcgagtc ggtggtggaa gtgctgcgca gccaggcgaa gcccgtcgag accaaggcgg
     1441 cgatcgccca ggtggcgagc atctccgcgc acgaggaggc gatcggccag ctgatctccg
     1501 acgcgatgga caaggtcggc aaggacggcg tgatcaccgt ggaggagtcc aaggggatcg
     1561 agaccacggt ggagacggtc gaggggatgc agtttgacaa gggctacatc tccccgtaca
     1621 tggtgaccga tccggagaag atggaggcgg tgctggacga tccgtacctc ctgatcacgg
     1681 acaagaaggt gagcgcggtc aaggatctcc tgccggcgct cgagcggatc gtccaggtga
     1741 gcaagccgct cctgctgatc gccgaagacg tggaaggcga ggccctcgcg acgctggtcg
     1801 tcaacaagtt gcgcggcacc ctgcaggcgg tcgcggtgaa ggcccccgcg ttcggggacc
     1861 ggcgcaaggc gatgctgcag gacatcgcga tcctgaccgg cggacaggtg atcaccgagg
     1921 aactgggctt gaagctcgac aacgtcgagc tcggcatgct gggcaaggcc cgccaggtca
     1981 agatcgggaa agaggagacg atcatcatcg gcggcgcggg caagcagtcg gagatccaga
     2041 agcggatcag cgagctgcgc aagcagatcg atgagaccga gagcgactac gaccgagaga
     2101 agctccagga gcggctcggg aagatggtgg ggggcgtcgg cgtgatcaag gtcggcgccg
     2161 ccagcgagac cgagctcaag gagaagaagc accgtgtgga agatgcgctc tccaccgcgc
     2221 gggcggcggt ggaggagggc atcgtgccgg gcggcggggt cgcgttgatc gcggcgatct
     2281 ccgccctcga aaaggtggac gcgacgggag acgagaaggt cggcgtggat atcgtccgcc
     2341 gggccctcga ggaaccgacg cgccaactgg cgcgcaacgc cggcgccgag gggtcgatca
     2401 tcgtcgagaa ggtgcgggcc agcaaggccg ggtgggggtt caacgtgctc tccggcgagt
     2461 tccaggatat gttcaaggcc gggatcgtcg acccgtgcaa ggtcacccgc tcggcgctcc
     2521 agaacgccgc gagcgtggcg tcgatgatcc tcacggccga ggtgctggtg gtcgacaagc
     2581 cggaggagga gaaggaggcc ccaatgcctc cgacgccgcc gatgtaggca ggagtgcccg
     2641 gggcggggga cgcggcgagc gaatccgggt gagccgagtc gattgaccgc tgaagccaga
     2701 cgcggggagg tctcgcaggc ctccccgcgt cgcatctggc cgatcgccgc cctcctgctc
     2761 ctcgcgctcg tgcgcggcgc gattgcctcg cgcacgccga tcatcgacga cgaaggatac
     2821 tactggctct ggtcccgcca tctcagcctg tcgtacctgg accaccctcc gctcgtggcg
     2881 tggctggatg cgctcgcgac ctccgcgggg cggaccgagt ggctgctccg gctgccggcg
     2941 atggtggcga ccctcggcac gacgctcgtc ctctacgggc tcggcggcga actgttcggc
     3001 ccccgggccg gcctccgcgc cgccgcgctg cacgcggctg tgccggtgtt cgcgctccag
     3061 gcgatccacg cgatccccga tccgctcctc tatctctggt gggccgccgc gatgtgggcg
     3121 ttctggcggg cggtgcacgg ggagccccgc atgtggtggg tctgcgggct ggcgctcggg
     3181 ctgggcgccc tcagcaagta cacggtgctc atcctcctca tcgccatgct cgggatgctc
     3241 cgctggccgc gctaccgccg gtggtggcgc acgccgggcc cgtaccttgc cggcgcgctc
     3301 atgtgtgccg tggcgttgcc ggtgctcgtc tggaacgccc agcacgggtg ggcctcgctg
     3361 cgcttctggc tcaacgcggg agggatgcag gggcccccgc cggggacggg caaggggtgg
     3421 gggctcccga gcgtcgccgc ggtgcagttc gcctacggcg ggccgctcct gtttcccgcg
     3481 atggtctggg cgctctggcg gagttggcgg atggcgcgcg acgatgagcg cttcgcattt
     3541 ctcgcgatgg ccggcggtcc ggtcctcttg ctcgtggccg gatcaagcct gctcggcgtg
     3601 gaccgcccga actggcccgc gccggcctac ctcgcggggg cgctggcgct cggcgcgctc
     3661 tggccccgcc tgctcgcccc cgcggcgttc gcctccagcc tggcgctgtc cctggccgtt
     3721 tcggtcctga tcaccctgat cccccagctt ccgcccgcgc tccaatggag cgaactgtac
     3781 ggctggcgcg aggtcacgca ggagatgctc cgccgcgcgg acgcgctgcg cgaccccggg
     3841 cacgtgctgc tgctcggcag gcggtacaac gaggcgagct acttcgggta ctatacgatg
     3901 gaccgggtgc cggtcagtac cgggcacctg agcgcgttga gcttttgggc gcccccgcac
     3961 cggttcgtcg gctggcaggg gatcgcggcg atcgaccccg cctccggcgt ggacgagttg
     4021 gccggggcct gcgcgcggct cgtcgagcaa cccccgtatt tcgtccggtt cgacggcggc
     4081 ggcggcagga ggttccggct gtttcactgc atcggatacc gcgggacgcc tcggccgccg
     4141 taggggcccc gatggcgagg aggctcccgc gaccgcgaga gggcacagtg ctcgaccgtc
     4201 ccctggtgag cttcgggcgc gaggtgtgcg gcgatctctc cgccgggctc cggcgggagt
     4261 ggctcgtgac gaacggcctc ggcgggtacg cgtccgggac cgtggccggg gtcaataccc
     4321 gccggtatca tgggctgctc gtggcggccc tcaccccgcc cgtcggacgc accgtgctcg
     4381 tgggcggcct cgtggaatgg gcgcgctacg acgggcgccg gtacccgctc gcgacccacg
     4441 agtacggcga tggcacggtg gacccgcgcg gataccgcca cctccagtca tttgcactcg
     4501 aggggatgct ccctgtgtgg gtttttgcgc tcggcgatgc gctgctcgag aagcgggtgt
     4561 ggatggccca cggcgccaac acgacgtacg tgatgtaccg gttggttcgc gccacccgcg
     4621 cggtggagtt ggagatcacc ccgctggtca cctatcgcga ctttcatacg ctgacgtcgg
     4681 gacgaggatg gcgacccgag gccgaggccg caccacacgg cgtggtgctc cgcgccttcc
     4741 cgggggcggt gccgttccgg ctcctgctgg aggggggtcg gttcgttgcc cgcggggact
     4801 ggtactggaa ctttcttcac cgcgaggaat ccgcccgagg gctggacgac cgcgcggacc
     4861 tgtacgcccc cggtgcgttc acggccgcgc tcacccccgg ggagagcctc accctggtcg
     4921 gaacggcgga cgccacgccc ccggcgggag gccctcgctc cctcgccgcg gttcaggccc
     4981 ggcagcagca cctgctgcgg cgcgcagggg tcacggactc caatccggtg gtgcagcaac
     5041 tcgttctcgc ggccgatcag ttcatcgtcc ggcggacgac cggtggccgg ggcgcgaccg
     5101 cagacgccgg acagacggtg atcgccggct accactggtt caatgactgg ggacgggata
     5161 ccatgatcgc gcttcccggt ctgaccctcg ccacggggcg cccggaggtc gccgcagaga
     5221 tcctgcgcac ctttacgcgg tacctggcgg acggcctcct ccccaacaac ttcccggatg
     5281 ccgccggggc cggtcccggg tacaacaccg ccgacgcatc gctctggtac gtgctcgccg
     5341 tgcgcgccta ccacgaggcc acgggggacg accaactggt cgacgaggtg ctgccggcgc
     5401 tgcgcgaggt gatcgaccgg cacgagcgcg gcacgcggta tgggatcggc atggatccgg
     5461 ccgatggcct cctccgggcc ggagcgccgg gggtgcagct gacctggatg gacgccaagg
     5521 tcggcgactg ggcggtgacc ccccgggccg gcaagccggt ggagatcaac gcgctctggt
     5581 acaatgcgct gcgcacgctc gccgccttcc tcgcctccag gcgcgacccc gccgcgccgc
     5641 gctaccgcgc gctggccgac cgggtgcgcg ccgcgttccg cgcccgcttc gccagccaag
     5701 gccgaggctc gctggccgat gtggtggacg gccccggcgg ggatgacctg gcggtccgtc
     5761 cgaaccagat cttcgccgtg tccctcccct tcccgctcct cgaacgcgcc gccgccgcgg
     5821 cggtcgtgga cgaggtagga cgcgcgctgc tcaccaccta cgggctccgc tcgctcagcc
     5881 cggacgaccc cgcctaccac ggggcgtacg gcggggatcc gccgcaccga gatcgtgcgt
     5941 accaccaggg gcctgcgtgg gcctggctcg cgggggcgta cgccgaagcg taccatcgcg
     6001 tgcacggaga ccttgagggc gcgcgtgcac tgctgcgtcc cttcgagcac cacctccgcg
     6061 atgccggact ggggaccatc tccgagctct tcgaaggcga cccgccgcac ctgccccgcg
     6121 ggtgcatcgc ccaggcgtgg agcgtcggcg aagtgctgcg cgtgtggcgg gtgctggaga
     6181 agggacgccc taggcgtggc gcctggtaga tagattgttt acacgtaagc ccaaatttcc
     6241 gatgatcatc tacgacctga ggaggagacc catgtccaag cttcgagact tcaccctgct
     6301 cagccctcga gctcaactgg acaggagctt ttatgacagg ttgagagctg ccaggcccca
     6361 atataggttg gctgagaggt tcgtcatccc tcccttcgaa ggacgcgggt tcatcgccac
     6421 gaagggccag gtgttcaggg tcatcgagga agagggcgtt cagatcggtg acatctgctt
     6481 ctggaacgcc cacaacgcga aggagatgta cagcctgtcc cggacctggg cgatagaggg
     6541 ctgggcggtc aggccctaca cccgcatctg gtccgatgtc ccgtggctcc gccccatggc
     6601 cacctgcctc gatgacacag tggtcccagc ctctgccgac gccggctttc accaccactg
     6661 gctcgggacc cactgtgcaa cggagtggac agagatgcgg gtgcgtatcc cgggcctgaa
     6721 ctcctgccat ctgaactttc ttcaggccat cacgcccttc ggattgaccg aggaagatat
     6781 tcgggacaac gtcaacctct tccaaaagat gcgcccggac cctgagacgg gaaggatctc
     6841 cgcggcattg agcgattcga agaaggggga ctac
//
LOCUS       DASQGH010000363         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24348, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000363 DASQGH010000000
VERSION     DASQGH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24348"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..381
                     /locus_tag="VEW91_07100"
                     /pseudo
     CDS             1..381
                     /locus_tag="VEW91_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            423..1835
                     /gene="murC"
                     /locus_tag="VEW91_07105"
     CDS             423..1835
                     /gene="murC"
                     /locus_tag="VEW91_07105"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HYM91385.1"
                     /translation="MMIPAGARVHFVGIGGAGMSAIASVLIARGYVVSGSDLRESEVT
                     RRLRTAGAQIQIGHAPEHVEPGQVVVISRAVPEENVEVQMARRRGVQVLHRAEMLAGL
                     MEGMRSIAVVGTHGKTTTTSMIARILEQAGRDPTVLIGGEVDDFGGNARAGRGEDLVA
                     EVDESDGSLLRVAPEIAVVTNTDATDHLDYYGSIERVLETFRRFLQRLPARGLAVICA
                     DSTVGRTLAEGVRGSGGARAVTFGLEPGADYAARILEMAGWKTAFEVRRGPDLLGRAT
                     LSVPGEYNVQNALGALAVGLEVGVPFASAASSLGTVRGVQRRFTVRGEVGGVLVVDDY
                     AHNPTKVRSLLLAARRCWPQSRIIAVFQPHRYSRTRTVGPQFADAFDAAHEVIVTELY
                     AADEPSVPGVDAGVIVRAVRARRPVEYIAEAAQVVASLAERVGPGDLVLTIGAGDVWK
                     IGDALLSSLRARAAGVQGHG"
     gene            1828..2778
                     /gene="murB"
                     /locus_tag="VEW91_07110"
     CDS             1828..2778
                     /gene="murB"
                     /locus_tag="VEW91_07110"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYM91386.1"
                     /translation="MGSVLQDTVLAALERLCPGGVCPNEPLARHVTFRIGGPADVLLV
                     PPSLDHLVAAVTWLYREGQPFVVLGRGSNVLIADRGVRGVVIKVGRGQDATRYQGNRV
                     MAQCGVSLPHLSRATATRGLAGLEFAAGIPGSVGGGIVMNAGAHGCSLAGVVRQVRVL
                     TPGGERTWSRDELHFQYRQSRLQHEPGVVLEAELELHPADPRECLGRLEAWLRTRSDT
                     QPLGPPSSGCVFRNPTGDHAGRLIDITGGKGMRVGGAAVSDRHANYILNTGGATAADV
                     LALVAQVRARVRDRSGTDLEPEIKLIGEFEPGEGAPEGSR"
     gene            2832..3635
                     /locus_tag="VEW91_07115"
     CDS             2832..3635
                     /locus_tag="VEW91_07115"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HYM91387.1"
                     /translation="MGLRRVARFLAAMVGLCGVAAFPASAIFDLRTIAVEGNLAVPTD
                     AVLHRAGLGPGTGAFRVNAAAIRERLLGDPRIEDVAVAMAFPHGLRVVIQERRPVAAL
                     VVGERTVLLSGDAVVIAESPDTGGLPALVVDRLDPAEVMPGRVLRAPDARFGAQIAGT
                     LPAPLRGRVVLVWITRDGEAVLRLREGISVRVGGPRGVMTRLEMVPQALDAMASRGLH
                     VEVVDLRFSGNIVIQPARSSDAPSPPPRGRQENLPARGIDPARHRPSNP"
     gene            3657..>4661
                     /gene="ftsA"
                     /locus_tag="VEW91_07120"
     CDS             3657..>4661
                     /gene="ftsA"
                     /locus_tag="VEW91_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HYM91388.1"
                     /translation="MAKREPLVGLDIGTTKVCVVVAEQGADGEVHITGMGTSPSAGVR
                     KGVVVDLDITTRAIEEAVDKAERMAGVRIAGATVGVSGGHLASQNSRGVVAVSRADHE
                     IGEHDVLRVVEAARMATVPASDREIVHLLPRDFIVDGQDGVKNPVGMYGTRLEVETHI
                     VTGASTLLANLLKCVQRAGLESDALVLESLASGEAVLSPAERELGVALVDIGGGTTSL
                     GIFTGGGLCYTQVLPYGGNHVTNDIGVGLRTSLDDAERLKLRHGCAVPAMATEGEMIE
                     VVHVGSREPRVMPKRVLAEIIQPRIAEIAGLIKAQLQRSGLAYRIPAGIVATGGTAGL
                     A"
BASE COUNT          627 a         1541 c         1818 g          675 t
ORIGIN      
        1 caggggtggg agggggcgga cggggggcgt ccggcggtgg gggcggccca ggggctccgg
       61 tacgtccgcg ttccctacct ggaccacatc ggggcggcgt acgcgtgcgc cgacctggtc
      121 gtgagccgct gtggcgccaa cgcgcttgcg gagatcacgg cgtgcgggtt gcccgccatt
      181 ctcgtgccgt atccccacgc cgcagacgac catcaggccc acaacgccag caggctcgtg
      241 tcggcggggg ccgccgtgct cgtgcccgac gcccaactca ccggcgaggt cctcgcgcgc
      301 gagatcgccg cggtcttcga tcggcccggg gtgctcgcgg cgatggccgc cgccgcccgg
      361 agccaggggc ggccggatgc ggccgaccgg gtggtcgcgt tggtctcccg gctggtggtt
      421 caatgatgat ccccgccggg gcgcgcgtgc acttcgtcgg catcggcggc gccgggatga
      481 gcgcgatcgc gtcggtgttg atcgcccggg gctacgtggt ctccggctcc gaccttcggg
      541 agagcgaggt cacgcggaga ctgcggaccg ccggcgcgca aatccagatc ggccacgccc
      601 ccgagcacgt ggagccgggg caagtcgtgg tcatcagccg ggccgtgccc gaggagaacg
      661 tcgaagtgca gatggcccgc cggcgcgggg tgcaggtgct gcaccgggcc gagatgctgg
      721 ccggcctcat ggagggcatg cggagcatcg ccgtcgtcgg gacccacggg aaaaccacca
      781 cgacgtcgat gatcgcgcgg atcctcgagc aggccggacg cgacccgacg gtgctgatcg
      841 gcggcgaagt cgacgacttc ggcggcaacg cgcgggccgg tcgcggggaa gacctggtcg
      901 cggaggtgga tgagagcgac gggtcgctgc tccgggtcgc gccagagatc gcggtcgtca
      961 ccaacaccga cgccaccgac cacctggatt actacggatc gatcgagcgg gtcctggaga
     1021 cgtttcggcg gtttctccag cgccttcccg cgcgcgggtt ggcggtcatc tgcgcggact
     1081 cgaccgtggg tcgcacgctc gccgaggggg tgcggggctc gggcggggcc cgcgcggtga
     1141 cgttcgggct ggagccgggg gcagactacg cggcccgcat cctcgagatg gccgggtgga
     1201 agacggcgtt cgaggtcagg cggggcccgg acctcctggg ccgggcgacg ctctccgtcc
     1261 ccggcgagta caacgtgcag aacgcgctcg gggcgctcgc cgtgggcctg gaggtcggcg
     1321 tgcccttcgc ctcggccgcc tcctccctcg ggacggttcg cggcgtgcag cggcggttca
     1381 ccgtgcgggg cgaggtcggg ggggtgctcg tggtcgacga ctacgcgcac aatccgacca
     1441 aggtgcgctc gctgctcttg gcggcccgcc ggtgctggcc ccagtcccgc atcatcgcgg
     1501 tctttcagcc gcaccggtac agccggaccc ggacggtggg cccccagttc gccgacgcct
     1561 tcgatgcggc gcacgaggtg atcgtgaccg agctgtatgc ggccgatgag ccgtcggttc
     1621 cgggggtcga cgccggggtc atcgtccgcg ccgtccgcgc gcgccggccg gtcgagtaca
     1681 tcgcggaggc cgcgcaggtg gtcgcgtccc tggccgagcg cgtcggcccc ggcgacctcg
     1741 tgctcacgat cggggccggg gacgtctgga agatcgggga cgcgctgctg tcgagccttc
     1801 gggcccgggc ggccggagtg caggggcatg ggtagcgtgc tgcaggacac cgtcctcgcc
     1861 gcgctcgaga ggttgtgccc gggaggcgtg tgccccaacg aacccctcgc ccggcacgtg
     1921 acgtttcgga tcgggggccc ggccgacgtg ctgctggtgc cgccgtcgct cgaccacctg
     1981 gtggccgccg tcacgtggct gtaccgggag ggccagccgt ttgtcgtgct ggggcggggg
     2041 agcaacgtgc tgatcgcgga tcgcggcgtg cgcggggtcg tcatcaaggt cggccggggc
     2101 caggacgcga cccggtacca gggaaaccgg gtcatggccc agtgcggggt cagcctgccg
     2161 cacctgagcc gggccaccgc aacgaggggg ctggcggggt tggagttcgc cgcggggatc
     2221 ccggggtccg tcggcggggg gatcgttatg aacgcgggcg cccacgggtg ttccctagcc
     2281 ggggtggtcc gtcaggtcag ggtgctgacc cccggcgggg aacgcacatg gtcgcgcgac
     2341 gaactgcatt tccagtaccg gcagagccgg ctccagcacg aacccggcgt cgtgctcgaa
     2401 gccgaactgg agctccaccc ggccgacccg cgcgaatgcc tgggacggct ggaggcatgg
     2461 cttaggacgc gcagcgacac gcagccgctc ggccccccga gttccggctg cgtcttccga
     2521 aatcccacgg gagatcacgc cggccggctg atcgatatca cgggcggaaa ggggatgcgg
     2581 gtcggcggcg cggcggtgag cgaccggcac gccaactaca tcctgaatac cgggggggcc
     2641 accgcggccg atgtcctggc gctcgtcgcc caggtgcgcg cccgggtccg ggatcgatcc
     2701 gggaccgacc tcgagcccga gatcaagttg atcggcgagt tcgagccggg cgagggcgcg
     2761 ccggagggat cccggtaggg tccacggtgg gtcccccccc gaccagtccc cctcccgtcg
     2821 gacggcgtgg ggtggggctg cggcgggtgg cacggttcct cgccgccatg gtggggttgt
     2881 gcggggtggc ggcgttccct gcctcggcga tctttgatct ccgcacgatc gccgtcgagg
     2941 gcaacctcgc ggtgcccacc gacgccgtcc tccaccgggc cgggctgggg ccggggaccg
     3001 gggcctttcg cgtcaacgcc gcggcgatcc gcgagaggct gctgggggat ccgcggattg
     3061 aagacgtcgc ggtcgcgatg gcgtttcccc acggcctccg ggtggtgatc caggagcgga
     3121 ggccggtggc cgccctcgtg gtcggagagc gcaccgtgct cctctccggg gacgcggtgg
     3181 tgattgcgga gagccccgac accggggggc ttcccgcgct cgtcgtcgac cgcctcgatc
     3241 cggccgaggt catgcccggc agggtcctgc gggcgccgga cgcgcggttt ggggcgcaga
     3301 tcgccggcac cttgcccgcg cctctgcggg gtcgggtcgt cctggtctgg atcacccggg
     3361 acggcgaggc ggtcctcagg ctgcgcgagg ggatctcggt gcgcgtgggc ggccccaggg
     3421 gcgtgatgac tcgtctggaa atggtcccgc aggcgctcga cgcgatggca tcgcgcggcc
     3481 tgcacgtcga ggtggtggat ctgcgattct ccgggaacat cgtcatccag ccggcgcgct
     3541 cctccgatgc gccatccccg ccaccgcgcg ggcggcagga gaaccttccg gcacgtggaa
     3601 tagatcccgc gaggcaccgt ccatcgaacc cttaatcgga cccggaggtg cacccgttgg
     3661 ccaaacggga gcccttggtg gggctcgaca tcggcaccac caaggtttgt gtagtcgtcg
     3721 cggagcaagg tgcagacgga gaggtccaca tcacagggat ggggacctct ccgtctgctg
     3781 gtgtccgaaa aggcgtggtg gtcgatctcg atatcaccac gcgggcgatc gaggaggcgg
     3841 tcgacaaggc cgagcggatg gccggggtga ggatcgccgg ggccaccgtc ggcgtgtcgg
     3901 gggggcacct cgcatcgcaa aatagccgcg gcgtggtcgc cgtcagccgg gccgatcacg
     3961 agatcggcga gcacgacgtc ctgcgggtcg tcgaagccgc gcggatggcg acggtccccg
     4021 ccagcgatcg cgagatcgtg cacctgctcc cgcgcgactt catcgtcgat ggacaggacg
     4081 gcgtgaagaa cccggtgggg atgtatggga cccggctgga ggtcgagacc cacatcgtca
     4141 ccggcgcgag cacgttgctt gcgaacctgt tgaagtgcgt ccagcgggcc ggattggaaa
     4201 gcgacgcgct cgtgctggag tcgctcgcct ccggcgaagc cgtgctctcg ccggcggaac
     4261 gggagctggg cgtggcgctc gtggacatcg gcggaggaac gacgagcctc gggatcttca
     4321 ccgggggcgg gctctgctac acccaggtgc tcccgtacgg gggcaaccat gtgacgaacg
     4381 acatcggggt cgggctgcgc acctcgctgg acgatgcgga acggctcaag ctccgccacg
     4441 ggtgcgcggt gccggccatg gccaccgagg gagagatgat cgaggtggtg cacgtgggga
     4501 gccgggagcc gcgggtcatg cccaagcggg tgctggccga gatcatccag ccgcggatcg
     4561 cggagatcgc gggcctgatc aaggcgcaac ttcaacgctc cggccttgcc taccggattc
     4621 cggccggcat cgtggccacg ggcggcacgg ccgggttggc g
//
LOCUS       DASQGH010000364         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21299, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000364 DASQGH010000000
VERSION     DASQGH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21299"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1091
                     /locus_tag="VEW91_07125"
     CDS             78..1091
                     /locus_tag="VEW91_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HYM91389.1"
                     /translation="MIVRSKAPLRISFAGGGTDVPPYPQERGGVVLSITIDKYAYCSL
                     TPNQQGAIRVSSLDYDVIANYRSTADLVYNGQLDLVKAAINVMGAPAGLDLFLHTDAP
                     PGSGLGSSSATVVALVGAFRHLLRLPLSTYEIAQQAYHIERIELQIQGGMQDQYAAAF
                     GGMNFIEFHPDAVIVNPLRVDPDTLNELEYHLVLCYTGRTRRSAHIIERQVASYIGRR
                     EEVVRALDGLKALTIQMKNALLRGHLHEFGALLHEGWKLKTGLDQEITDSHIDELYDV
                     ARRHGAIGGKLLGAGGGGHFLFYCDFDKKHVLAEKLETLGAKIVEFGFDLRGLQTWQA
                     NNS"
     gene            1104..1721
                     /locus_tag="VEW91_07130"
     CDS             1104..1721
                     /locus_tag="VEW91_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HYM91390.1"
                     /translation="MSSDQSIIGQLRESANVTLMIGARQVAIIRHMADTIIAAYRTGK
                     KVVTCGNGGSAAEAQHIAAELVGRLQLPDRPPLAAVALTTDSSVLTAVGNDFGFEQVF
                     ARQVEACVHKGDVVIALSTSGNSPNVLAAVEKAKSLGATTIGLTGTPGGALADAVDLV
                     LAVPSESTQRIQEAHLTVGHIVCSIVESELFGVRRHERVGPARRR"
     gene            1868..2542
                     /locus_tag="VEW91_07135"
     CDS             1868..2542
                     /locus_tag="VEW91_07135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYM91391.1"
                     /translation="MTYARPPERRPIAARRWGSARALAGWLARRGVSPNAVSIWGMLC
                     GLGAGAALAATALVPSYVRVAWVSAAALILLRATANMLDGMVAVEFGRSSPVGELYNE
                     VPDRVSDAVMLIGLGYAAGGLSWLGYAAACAALLVAYVRAVGRAAGVPQEFGGPMAKQ
                     RRMFTVVAVGLYLGLEPRGWQPQWGAAPPVGLATVALAAISLGCLWTILWRLKRIALA
                     LQHPGA"
     gene            2546..3496
                     /locus_tag="VEW91_07140"
     CDS             2546..3496
                     /locus_tag="VEW91_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYM91392.1"
                     /translation="MPGISARWQSLSPIGQMGLIVFGLLVVATGIVGALCRAAMVRDT
                     SELVARTRSWWWMAAVVFAAVAIDTRLALLLFAFLSFWALKEYVTLLRTRPADHRALV
                     WAFLAIPVQYYWIATGAYIMFLIFIPVYMFLWIPLRLVLAREMVGFVASASAIQWGLM
                     AFVLGLSHLGYLATLPPFAGTRVDGRTLVLFLVFVTEISDVFQYVWGKVLGRHKILPT
                     VSPHKTWEGFLGGVTTAAVFGVLLRFLTPFGPGEAFVVSLLVTVAGFCGGAVMSAVKR
                     DVGVKDFGSLIPGHGGMIDRVDSLCYAAPVFFHYVQYFHT"
     gene            3474..>4047
                     /locus_tag="VEW91_07145"
     CDS             3474..>4047
                     /locus_tag="VEW91_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYM91393.1"
                     /translation="MSSTFTRRLRDLAQILFFAAVVHPFVTLFIGLRVRGRHNLPGRD
                     PFILIANHSSHLDTVSLLSLFPLGRLRKIHPCAAADYFGRTRLTACLSHTFFNVLLVA
                     RVKITPDTNPIPRMREALGRGESLILYPEGSRGSGETMAAFKTGIAHLLEEVPGVPVV
                     PAYLVNMGRSLPKGEYLPVPFICEVRIGVPR"
BASE COUNT          607 a         1338 c         1363 g          739 t
ORIGIN      
        1 gctgcggggc aggaactcat ggtgggtgat catgtgccgt gacggtaagg ccgggcgttg
       61 gcctggaggg accctaggtg atcgtgcgga gcaaagcgcc gctccgtatc agcttcgcgg
      121 gcggcggcac cgacgtccct ccctatccgc aagagcgcgg gggggtcgtc ctcagcatca
      181 cgatcgataa gtacgcgtac tgcagcctca ccccaaatca gcagggagcc atccgcgtct
      241 cgtcgctcga ctacgacgtg attgccaact atcggtcgac ggcggatctc gtgtacaatg
      301 gccaactgga cctcgtcaaa gccgcgatca atgtcatggg ggcgccggcc ggcctggacc
      361 tgtttctgca caccgacgcc ccgccgggat ccgggctcgg ctcgtcctcg gcgaccgtgg
      421 tcgccctcgt cggcgcgttc cggcacctgc tgcggctgcc cctcagtacc tacgagattg
      481 cgcagcaagc gtaccacatt gagcgcatcg agttgcagat ccaggggggg atgcaggacc
      541 agtacgcggc cgcgttcggg ggcatgaact tcatcgagtt ccatcccgac gcggtgatcg
      601 tgaatcctct ccgcgtcgat cccgatacgc tcaatgaact ggagtaccac ctcgtgctgt
      661 gctatacggg aaggacgcgc cgctccgccc acatcatcga acgccaggtc gcctcctaca
      721 tcggacggcg ggaggaggtt gtccgagcgc tcgacggctt gaaagccctg accattcaga
      781 tgaagaacgc gctgctccgg gggcatctcc acgagttcgg cgcgctgctc cacgagggct
      841 ggaagctcaa gaccgggctc gaccaggaga tcaccgattc gcacatcgac gaactctatg
      901 atgttgcccg gcggcacggc gccatcggcg gcaagctcct cggggccggg ggcgggggac
      961 attttctctt ttactgcgac ttcgacaaga agcacgtgct ggcggagaag ctcgagactc
     1021 tgggcgccaa gatcgtcgag ttcggcttcg acctccgcgg cctccagaca tggcaggcca
     1081 acaactcgtg agcgtgggtt agcgtgtcga gcgatcagag cattatcggg caactgcggg
     1141 agagcgccaa cgtcacgctg atgatcgggg cgcggcaggt ggcgatcatc cgccacatgg
     1201 cggacacgat catcgcggcg taccgaaccg gaaagaaagt ggtgacctgc gggaacgggg
     1261 gcagcgcggc cgaggcccag cacatcgccg ccgaactcgt gggacggctg caattgccag
     1321 accggccccc cctggcggct gtggcgctga ccacggactc gtcggtgttg accgcggtgg
     1381 ggaacgattt tggattcgag caggtgtttg cccggcaagt tgaggcctgc gtccacaaag
     1441 gcgacgtcgt gatcgcgctg agtacgagcg gcaactcgcc caacgtcctt gccgcggtgg
     1501 aaaaggccaa gagcctgggg gcgacgacga taggcctgac gggcaccccg ggtggtgcgc
     1561 ttgcggacgc cgttgatctt gtgctcgcgg tgccctcgga atcgacccaa cggattcagg
     1621 aggcccatct tacagtaggg cacatcgtct gctcgatcgt cgaatcagag ctgttcggcg
     1681 ttcggcgcca cgagcgggtt gggccggcca ggcgcaggtg agccagggct gctggccgaa
     1741 gccacgcccc ggttgaaaga agcgctgcag gatctgactt gcgcaccctc gaaaaattgc
     1801 gcacaattca ccccttttct cataattttc agatgtgcac acacccatac ccagcgcaac
     1861 cccggacatg acctacgccc gccccccgga acgccggccg atcgctgcgc gccgctgggg
     1921 gagtgcccgg gcgctggcgg ggtggctcgc acgacgcggc gtctcaccga acgctgtctc
     1981 gatctggggg atgctgtgcg ggctcggcgc gggggcggcg ctcgccgcta ctgcgctcgt
     2041 cccatcttac gtgcgggtgg cctgggtgag tgcggcggcg ctgatcctgc tccgcgccac
     2101 cgcgaacatg ctggacggga tggtggccgt cgagttcggc cgctcctctc cagtggggga
     2161 actctacaac gaggtgccgg atcgcgtctc ggatgcggtg atgctgatcg ggttggggta
     2221 cgcagcgggc ggactctcct ggctcggcta cgccgcggcg tgcgccgccc tcctcgtggc
     2281 ctacgtccgg gcggtgggcc gggcggccgg ggtccctcag gagttcggcg gccccatggc
     2341 caagcagcgc cggatgttca ccgtggtcgc agtcggcctc tacctggggc ttgagccgcg
     2401 gggctggcag ccccaatggg gagcggcccc cccggtcgga ttggcgaccg tcgccctcgc
     2461 cgcgatctcc ctcggctgcc tgtggacgat cctctggcgc ctcaagagga tcgcgttggc
     2521 cctgcagcac ccgggggcgt gagcgatgcc agggatcagc gcacggtggc agagcctctc
     2581 cccaatcggc cagatggggc tcatcgtctt cggcctgctg gtcgtcgcca ccggcatcgt
     2641 gggggcgctg tgccgcgccg ccatggtgcg ggatacgagc gaactggtgg cccgcacgcg
     2701 ctcctggtgg tggatggcgg cggtggtctt cgccgccgtg gcgatcgaca cccggctggc
     2761 cctgctcctg ttcgccttcc tgagcttctg ggcgctcaag gagtacgtca ccctgctcag
     2821 gacccgcccc gccgatcacc gggcgctcgt gtgggcgttc ctcgccatcc cggtccagta
     2881 ctactggatc gcgacgggcg cctacatcat gttcctcatc ttcatcccgg tctacatgtt
     2941 cctgtggatc ccgctgcggt tggtcctggc ccgagagatg gtcgggttcg tcgcgtcggc
     3001 gtcggcgatc cagtggggcc tcatggcgtt cgtcctcggg ttgagccatc tcggctacct
     3061 ggcgaccctg ccccccttcg ccggcacccg ggtggacggc cgcaccctgg tgctgttcct
     3121 ggtcttcgtc accgagatca gcgacgtctt tcagtacgtc tggggtaagg tgctcggccg
     3181 ccacaagatc ctgccgaccg tcagcccgca caagacctgg gaggggttcc tcgggggggt
     3241 gaccacggcg gcggtattcg gcgtcctgct gaggttcttg accccgttcg gccctgggga
     3301 ggcctttgtc gtctcgctcc tcgtgacggt cgccggcttc tgcggcggtg ccgtgatgtc
     3361 cgcggtgaag cgcgatgtcg gcgtcaagga ttttggttcg ctcattccgg gacacggagg
     3421 catgatcgac cgcgtcgact cgctctgcta cgcggcaccg gtgttcttcc actatgtcca
     3481 gtactttcac acgtaggctg cgcgacctgg cgcagatcct cttcttcgcg gccgtcgtgc
     3541 atccgttcgt gaccctgttc atcggcctgc gcgtgcgcgg ccgtcacaac ctgcccggac
     3601 gggatccatt catcctcatc gccaaccatt cgagccacct ggacacggtg tcgctgctca
     3661 gcctgtttcc gctcgggcgg ttgcggaaga tccacccgtg cgccgccgcc gactactttg
     3721 ggcgcacgcg gctgaccgcg tgcctctcgc ataccttctt caacgtcctg ctggtcgcga
     3781 gggtgaagat cacgccggac accaatccca tcccaaggat gcgggaggcg ctcgggcggg
     3841 gggaatcgct catcctctac ccggagggct cgcggggctc cggcgagacg atggcggcgt
     3901 tcaagaccgg gatcgcgcac ctgctcgagg aggtccccgg cgtcccggtc gtccccgcct
     3961 acctcgtgaa catgggccgc agcctcccga agggcgagta cctgcccgtg cctttcatct
     4021 gcgaggtgcg catcggagtg ccgcggg
//
LOCUS       DASQGH010000365         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18625, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000365 DASQGH010000000
VERSION     DASQGH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18625"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(520..2112)
                     /locus_tag="VEW91_07150"
     CDS             complement(520..2112)
                     /locus_tag="VEW91_07150"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYM91394.1"
                     /translation="MGEPLPVGQGGPAERYAGNWKTWILGSGGEIRLPPPAAPTSPQG
                     TIELAQLRRAQLERTEAQISAARYWDAGPATSRWTEICLDMIKMHRPNPPRAARGLAL
                     VHLALYDALVAAWHAKYLYNQPAPSSVDPMVAPVVRSRDNPSYPSEHAVAAGAASRVL
                     KHLFPQQGPEWFEAKAQEAAMSRVWAGADWPGAVEQGLALGRTVAERVLARAATDGSD
                     TPWDGKRLTGVCYWKPTPPGFVFPPLEPSWGKVKPWLLASAAHLRPGPPPLCGSAEER
                     EQFLEVYRTVNGLTEEQKRIASFWNDGPGTFTPSGHWFEIALGLAERYNINTPRTARV
                     FAYLGVVVMAVGVCVWDCKNAFWSLRPITYIQDYVDPHWTSFIPTPPFPGYVSGHSGF
                     SGGAAEVLGYAFPQERAALRSKAAEAAMSRLYGGIHTRADSEVGLAMGTRIGALASAR
                     ALSDDVEDLVGKGVLDRRQGDSLNARLGAAVMRVNQKNSKAAIGELQAFVSQVNAVLK
                     PADGDPLIGAAREIIAQFAAAN"
     gene            complement(2372..>2570)
                     /locus_tag="VEW91_07155"
     CDS             complement(2372..>2570)
                     /locus_tag="VEW91_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HYM91395.1"
                     /translation="PDWGEGQIISIWDGDERPLRTGMTFHLVPGVYDLGRHTIIISET
                     VLVRQTGCEVITNFPRDLFVA"
BASE COUNT          410 a          863 c          841 g          456 t
ORIGIN      
        1 cagacgattc gcatacgatc ccggtatggt tgtgctgctc tggcccaacg gactcttggc
       61 tcggatcgcc acgtttcgga agtacgaaat catcacctac cttgcggtcg ttccaccgcg
      121 gaccgccata ccgccgtacc acttccttgc cgcgctcccg ccgtctgaat gacatgcata
      181 cgccctcgac gactgggaag gtcgatcgga gacagaaggt ggctgagcct cgagaacgcg
      241 tttcgctcga ggccgaccca cttgacccgg tcccgccatt cagcggacct cggcccggcg
      301 acgaattccg cccctccagc ggcccgcaaa tcgcaaccga tctgcaatca cgcctctcgg
      361 gcgcagcctt ccttggcttc gggctcatcg gcacagcggt cgtcacacac ctgatcttct
      421 tccgccgcgc aaaacggcac ctcggcttct aatgccgggg cggcgtttcg ccatccagcc
      481 tactcctggt atccggagag cgcgtcacgc tagtggagac tagttcgcgg cggcgaattg
      541 cgcgatgatc tcccgggccg caccaatgag ggggtctccg tctgccggct tgagtacggc
      601 gttcacctgg ctgacaaagg cctgaagttc gccgatcgcc gctttcgagt tcttctggtt
      661 cactcgcatg accgctgccc cgagccttgc gttcaacgaa tccccctggc gccgatccag
      721 cacgcccttg ccgaccaggt cttcgacgtc gtcgctcagg gcgcgcgcgc tggcaagggc
      781 gccgatgcgc gtgcccatgg ccagccccac ctcgctgtcg gcccgcgtgt ggatgccgcc
      841 gtagagtcgg gacatcgctg cctcggccgc cttggaccgg agagccgccc tctcctgggg
      901 gaaggcatat cccagcacct ctgctgcgcc ccctgagaag cccgagtgtc cggatacgta
      961 cccggggaag ggcggggttg ggatgaagct ggtccagtga gggtctacgt aatcctggat
     1021 gtaggtgatc ggccgcaggc tccaaaaggc gttcttgcag tcccagacac agacgccgac
     1081 ggccatcacg acgacgccga ggtatgcgaa gacgcgtgcc gtgcgcggcg tgttgatgtt
     1141 gtatcgctcc gccaggccga gcgcgatctc gaaccagtgg ccggacggcg tgaacgtgcc
     1201 cgggccgtcg ttccagaagg aagcgatgcg cttctgctcc tcggtgaggc cgttgacggt
     1261 ccgatacacc tcgagaaact gttcgcgctc ttccgcggaa ccgcacaggg gcggaggccc
     1321 ggggcggaga tgggcggcgc tcgccagaag ccagggtttg accttccccc aagaaggttc
     1381 gaggggaggg aagacaaagc cgggcggcgt gggcttccag tagcagacgc ccgtcaagcg
     1441 cttcccgtcc catggggtat cggagccgtc tgtggcggcg cgcgccagga cccgctccgc
     1501 cacggtgcgg ccgagggcga ggccctgctc cacggcgccc ggccagtcgg cgccggccca
     1561 gacgcgcgac atcgccgcct cctgggcctt cgcctcgaac cactccggcc cttgctgcgg
     1621 gaacaggtgt ttcagcaccc gggaggcggc ccccgcggcc acggcatgct cggagggata
     1681 ggatgggttg tcccgggatc gcacgacggg cgccaccatg ggatccacgc tgctgggggc
     1741 gggctggttg tagagatact tggcgtgcca ggcggcaacc agggcgtcgt acagagcaag
     1801 atgcaccagc gcgagaccgc gggcagcgcg cggcggattg ggtctgtgca tcttgatcat
     1861 atccaagcag atctcggtcc accggctggt agccgggccc gcgtcccagt agcgagcggc
     1921 gctgatctgg gcctcggtcc gctcgagctg ggctcgtctg agctgcgcga gctcgatggt
     1981 tccctggggg ctcgtggggg ctgcgggagg ggggaggcgg atctcaccac ccgaacccag
     2041 gatccacgtc ttccagttgc ccgcatagcg ctccgcgggc cccccttgcc cgacgggcag
     2101 cggctccccc acgccggccg cggcatgcgc cgcctcgggc ctcttaccac tcaggaacca
     2161 gggaaggacg cgggcgcctg acccgattat cgcggcagcg atcgctgcat cacggaggaa
     2221 ctcgcgccga cccttccggg tttggctctg agggtgatct ttctcgccca cgcggatctt
     2281 ctgccctttt ggtcgcagag agcttttgat tcccccgttc gctatcagcc gtgaaagact
     2341 ccttcatcct tgcatgtcgc ggcggtggtt atcaggcgac gaacagatcc cgtggaaagt
     2401 tcgtgatcac ttcgcagccg gtctggcgga cgagcacggt ttcgctgatg atgatcgtgt
     2461 gtcgaccgag atcatagacc ccgggaacga ggtggaaggt catgccggtc cggaggggcc
     2521 gttcgtctcc atcccagatc gatatgatct gtccctcgcc ccagtcgggc
//
LOCUS       DASQGH010000366         1796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1554, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000366 DASQGH010000000
VERSION     DASQGH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VEW91_07160"
     CDS             <1..428
                     /locus_tag="VEW91_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91396.1"
                     /translation="WRSSGVYTETHAVSAAALFGQNGPQWNGKDAALIFTWGQGGFPE
                     NKINWHSSFIPKDATAPGENDERYSTPEMDRLLEEADRTVDEAKRHAIYNRIQELEFR
                     DVPIIFLYWLVNNNAVAKTVQGYDVTTFNTTPPEEWSTP"
     gene            463..1413
                     /locus_tag="VEW91_07165"
     CDS             463..1413
                     /locus_tag="VEW91_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91397.1"
                     /translation="MSSYVAQRVVQAGALLLLVSLVGYAIMNLAPGGPLAVYLHNPQV
                     TPEKIDLLRHQLGLDRPWYLRYAAWLAGLVRGHWGDSYYTGRPVLEMIAERLPATLTL
                     MLSAFVLALALSFPLGILAATHKYSWGDNLVSLGAFFVWAMPTFWFGLMLQMTFAVRW
                     HLLPVAGMHEIDNQSIGDLLRHLAMPAVVLGVGSIASWSRYLRSGMLEVLGQDYVRTA
                     YAKGLTAHRVVNRHVLRNSLLPIVTIMGLDLHVLFSGAVITESIFGWPGMGRLFLAAL
                     NNRDYPLQMAGLMVSAALLIVGNLLADLTYAALDPRIRYR"
     gene            1423..>1796
                     /locus_tag="VEW91_07170"
     CDS             1423..>1796
                     /locus_tag="VEW91_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HYM91398.1"
                     /translation="MNGLTATPAAPLIPPSGGGLSALAWRRFRRHRMALCAVGVLAVI
                     VTSAVLAPLITSYDPTALDPDHSLAPPSRQHLLGTDDLGRDELARVVYGGRISLLVGL
                     TAMLLGSAIGVLMGSAGGYMGG"
BASE COUNT          272 a          619 c          604 g          301 t
ORIGIN      
        1 cctggcggtc gagcggcgtg tacaccgaga cccacgcggt gagcgccgcc gcgctgtttg
       61 gccagaacgg gccccagtgg aacggcaagg acgcggcgct gatctttacc tgggggcagg
      121 ggggcttccc cgaaaacaag atcaactggc actcgagctt catccccaag gacgcgacgg
      181 ccccggggga aaacgacgag cggtacagca cccccgagat ggaccgccta ctggaggagg
      241 ccgaccggac ggtggacgag gccaagcgcc acgcgatcta caacaggatc caggaactgg
      301 agttccgcga cgtcccgatc atcttcctgt actggctcgt caacaataac gccgtcgcca
      361 agaccgtgca gggctacgac gtgacgacgt tcaacacgac gccgcctgag gagtggagca
      421 ccccttagcg gcccggccgg gcgatcctcg cggaggcatg agatgagctc ctacgtggcg
      481 cagcgcgtgg tacaggccgg cgccttgctg ctcctcgtgt cgctcgtcgg ctacgcaatc
      541 atgaacctcg ccccaggtgg cccgctggcg gtctacctcc acaatcccca ggtcacccct
      601 gagaagatcg atctcctgcg gcaccagctc gggttggacc ggccctggta cctgcggtac
      661 gccgcgtggc tggcggggct ggtgcgcggg cactgggggg actcgtacta caccggccgg
      721 ccggtgctcg agatgatcgc ggaacggctt cccgcgacgc tcacgttgat gctcagcgcc
      781 tttgtcctcg ccctggcgct ctcgttcccc ctcgggattc tcgcggccac ccacaagtat
      841 tcgtgggggg acaacctcgt gtcgctcggg gcgttctttg tctgggcgat gccaaccttc
      901 tggttcggcc tgatgttgca gatgacgttc gcggtgcggt ggcacctcct gccggtggcc
      961 ggcatgcacg agatcgacaa ccagtcgatc ggggacctgc tgcgccacct ggcgatgccg
     1021 gcggtggtgc tcggcgtcgg ctcgatcgct agctggagcc ggtacctgcg cagcggcatg
     1081 ctcgaggtgc tcgggcagga ttatgtccgc acggcgtacg cgaaggggct gaccgcgcac
     1141 cgcgtcgtga accgacacgt gctgcggaac tcgctcctgc cgatcgtgac catcatgggg
     1201 ctggaccttc acgtcctctt cagcggcgcg gtcatcaccg aatcgatctt cgggtggccc
     1261 gggatggggc ggctctttct cgccgcgctc aacaaccgcg actatccgct gcagatggcc
     1321 gggctcatgg tcagcgctgc cctgctcatc gtcggcaacc tgctggccga cctcacgtac
     1381 gcggccctcg acccgcggat caggtaccgg tagctacagc agatgaacgg cctgaccgct
     1441 accccggcag ccccgctcat cccgccgtcc gggggcggtc tctcggcact ggcctggcgc
     1501 cggttccgcc gccaccggat ggccctctgc gcggtcggcg tcctcgcggt gatcgtcacc
     1561 agcgcggtcc tggcgccgct aatcacgtcc tacgacccga ccgcgctgga ccccgatcac
     1621 agcctggccc cgccgagccg ccagcacctc ctcggcaccg acgacctggg ccgggacgag
     1681 ctcgcccggg tggtctacgg aggccggatc agcctgctcg tgggcttgac agcgatgctg
     1741 ctggggagcg cgattggggt cctgatgggt tccgcggggg gctacatggg ggggtg
//
LOCUS       DASQGH010000367         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4480, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000367 DASQGH010000000
VERSION     DASQGH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4480"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VEW91_07175"
     CDS             complement(<1..751)
                     /locus_tag="VEW91_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HYM91399.1"
                     /translation="MDYEDMEAEVLKGFVRDRQLITIALNHGFSPELLPSPVARRLCN
                     ALIDLYLARGGEAINDVTVRNHLETRGVLNAEMEHYLRAVLAMRPPDAGRMMSYVDSL
                     RNRDSRERLQTLHDQLGGFLGGTEDARGDLIQFTTEMIHKLIELQKRRVRQRFGPVTD
                     VLTALTEDVARSSGAGGILGYSISPFDRLNALLSGLRRGFYYALAGAPRRGKSNFALE
                     LATYVAGNHHVPCLYYSWEQTARVLAARLLAK"
     gene            complement(939..>1987)
                     /locus_tag="VEW91_07180"
     CDS             complement(939..>1987)
                     /locus_tag="VEW91_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYM91400.1"
                     /translation="QDVMSPPKSVQAPLISRIKILADMDIAERRLPQDGHIHLRAEGK
                     EYALRVSSLPTVLGEKVVLRVLDQSTTKVALDEIGFTTALLATWENLITKPYGFILVT
                     GPTGSGKTTTLYTSLTRINTPERNIVSVENPVEYQVPRVNQVQINPKVGLTFASGLRT
                     ILRQDPDIVLIGEIRDRETAEIAVQASMTGHLVLSTIHTNDAPGAVVRLRDMGIEPFL
                     ITSSLIGVLAQRLVRVICPKCKEAYVPPPDALKRLGVDVEKRADIRLFRGAGCEACRK
                     TGYKGRIGVFELMVMNERLCALAVTGAPTEQLRDAAIEEGMRTLKQDAIQKVLEGITT
                     FEEMLRVVFVSDAV"
BASE COUNT          302 a          661 c          682 g          342 t
ORIGIN      
        1 ccttggccag cagccgcgcc gcgagcacgc gggcggtctg ctcccacgag tagtacaggc
       61 agggcacgtg gtggttgccc gcgacgtacg tggccagctc gagggcaaag ttgctcttcc
      121 cgcgccgggg cgccccggcg agtgcgtagt agaatccgcg gcgcaaaccg gacagcaggg
      181 cgttgaggcg gtcaaagggg ctgatcgaat accccaggat gccgcccgct ccggacgacc
      241 gtgccacatc ctccgtcagg gcggtgagca cgtcggtgac gggcccgaac cgctggcgca
      301 ccctgcgctt ctgcagttcg atgagcttgt ggatcatctc ggtcgtgaac tggatgaggt
      361 cgccgcgggc gtcttcggtc ccgcccagaa agccgccgag ctggtcgtgg agcgtctgca
      421 gccgctcgcg gctgtcgcgg ttgcgcaacg agtcgacata ggacatcatc cggccggcgt
      481 cgggggggcg catcgccagc acggcgcgca ggtagtgttc catctcggcg ttgagcacgc
      541 cccgagtctc caggtgattg cgcaccgtca catcgttgat cgcctctccc ccgcgcgcca
      601 ggtacaggtc gatcagggcg ttgcacagac gccgggccac cggggagggc agcaactcag
      661 gggagaaccc atggttcagc gcgatcgtga tgagttgccg atcccgcacg aaccctttca
      721 ggacctcggc ttccatatct tcgtagtcca tacgccctcc cgatgcaaaa gcccgtgcgc
      781 cggcgcgaac gggacgccgc gcggctcagc ccgtcgtgcg gtcctgcgag ttgatggggc
      841 gcgagtcagc accctgtact gtgccctgaa ttgcccgccc agcggacacc cgcccgccgc
      901 ccccccaggg gggatcgccg cccgtggggc ggccggcgct acaccgcatc gctgacgaac
      961 accacgcgca gcatctcctc gaaggtggtg atcccttcca gcaccttctg gatggcgtcc
     1021 tgtttgaggg tccgcatccc ctcctcgatc gccgcatccc gcaactgttc ggtgggggcg
     1081 ccggtcacgg ccagggcgca caggcgctcg ttcatgacca tgagctcgaa gacgccgatc
     1141 cgtcctttgt atccggtctt ccggcacgcc tcgcacccgg ccccccggaa gagccggatg
     1201 tccgcccgct tctcgacgtc gacgccgagc cgcttcagcg cgtccggcgg aggcacgtag
     1261 gcttccttgc acttggggca gatcacccgg acgagccgct gggcgagcac cccgatcagc
     1321 gacgaggtga tcaggaacgg ctcgatcccc atgtcccgga gccggaccac ggccccgggg
     1381 gcgtcgttcg tatggatcgt gctcagcacg aggtggccgg tcatcgaggc ctggaccgcg
     1441 atctcggccg tctcgcggtc ccggatctcg ccgatcagca cgatgtcggg gtcctgccgg
     1501 agaatggtcc tgaggccgct ggcaaacgtc aggccgacct tggggttgat ctggacctgg
     1561 ttgacccgtg ggacctggta ctcgaccggg ttctccacgc tgacgatgtt gcgctcgggg
     1621 gtgttgatcc gcgtgagcga ggtgtagagg gtcgtcgtct tcccgctgcc ggtcggcccc
     1681 gtgacgagga tgaagccgta gggcttggtg atgaggttct cccaggtcgc aaggagggcc
     1741 gtcgtgaacc cgatctcgtc gagggcgacc ttggtcgtgc tctggtcgag cacgcggagc
     1801 acgacttttt cgccgaggac ggtaggcagc gagctgacac gcagggcgta ttccttgcct
     1861 tccgcccgga gatggatgtg cccatcctgc gggaggcggc gttccgcgat gtccatatcg
     1921 gcgaggatct tgatccgcga gatgagcggg gcctggacgg acttcggcgg ggacatcacg
     1981 tcctgga
//
LOCUS       DASQGH010000368         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49739, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000368 DASQGH010000000
VERSION     DASQGH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49739"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VEW91_07185"
     CDS             <1..750
                     /locus_tag="VEW91_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91401.1"
                     /translation="EPLIEVRDLTKVYGEGTTAVHALRGVTVLIRPGEFVAIMGPSGS
                     GKSTFMHLLGCLDRPTSGSYRLAGQEVSRLSGDQLADIRNKRIGFVFQTFNLLARTTA
                     LENVELPMLYAGLGREERLRRARALLGQVGLGDRLDHRPAELSGGQQQRVAIARALAN
                     GAPLVMADEPTGNLDSVSSGELMTLFRHLNEQGITIVVVTHDTNIAAWAKRVVTFKDG
                     RIVDDRPVDEVIPESARRAAVGAAAARGARA"
     gene            747..1973
                     /locus_tag="VEW91_07190"
     CDS             747..1973
                     /locus_tag="VEW91_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91402.1"
                     /translation="MNVTLLLRVAWQALRRNATRSFLTMLGIIIGVGAVITSMAIGSG
                     AQAAVLAQIESLGANLVVVIPGAVTTGGVTLGFGTKTSLRLADVAAIAQQVPEVAAAA
                     PSSSTSGQVVAGGLNWFTSVSGSTPAWQTVRNWPVAQGRFFTTAEVEQVAKVAVLGST
                     VAQNLFPAGGAVGSTVVIKNVPVHVIGVMASKGQSGFGRDQDDFAMVPITTLQERLTG
                     VTFNRSVSAILISATSPETVQGVIDETERLLRIRHHLTPGQPDDFSVRNISDIQQVRI
                     ATSQTQALLLAATAVVSLVVGGIGIMNIMLVSVTERTREIGLRMAVGAKGRDILLQFL
                     VEALTLASIGGLIGIAMGLATAQGASTLGHWPVLVSPFSMILGFASAALVGSVFGFYP
                     AQRAAALDPIVALRYE"
BASE COUNT          293 a          707 c          709 g          322 t
ORIGIN      
        1 gagcccctca tcgaggttcg ggacctgacg aaggtctacg gggagggcac caccgcggtc
       61 cacgccctcc gcggcgtcac ggttttgatc cggcccggcg agttcgtcgc gatcatgggc
      121 ccatcgggct ccgggaaatc gacgttcatg cacctgctgg ggtgcctgga ccggccgaca
      181 tcgggctcgt accggctcgc cggccaggag gtgtcgcgtt tgtcgggcga ccagctggcc
      241 gacatccgga acaagcggat cggcttcgtc ttccaaacct tcaacctgct cgcccgcacc
      301 accgccctgg agaacgtgga gctgccgatg ctgtacgccg gcctgggccg cgaggagcgc
      361 ctgcgccggg cgcgcgcgct tctcggccag gtggggctcg gagaccgcct ggatcaccgg
      421 ccggccgaac tctccggcgg ccagcagcag cgcgtggcca tcgcccgggc gctggccaac
      481 ggggcgccgc tcgtgatggc cgatgagccg accgggaacc tggacagcgt gagcagcggt
      541 gagctcatga cgctgttccg gcacctcaac gagcagggca tcaccatcgt ggtcgtcacg
      601 cacgacacca atatcgccgc gtgggcgaag cgcgtcgtga ccttcaagga tggccggatc
      661 gtcgacgacc gtccggtgga cgaggtgatc ccggagtccg ccaggcgggc ggccgttggc
      721 gcggcggccg cccggggggc gcgggcatga acgtcacgct gctgctgcgt gtcgcctggc
      781 aggcgctccg gcgcaacgct acgcgctcgt tcctgacgat gctggggatc atcatcggcg
      841 tcggggccgt gatcacctcg atggccatcg gctccggcgc gcaggcggcc gtgctcgcgc
      901 agatcgagag cctgggcgcc aacctcgtcg tcgtcattcc gggcgccgtc accaccggag
      961 gagtcaccct cgggttcggg accaagacct cgctccgcct cgccgacgtg gcggccatcg
     1021 cccagcaggt ccccgaggtg gccgctgcgg ccccgtcgtc ctcgacgagc gggcaggtcg
     1081 tggccggagg cctgaactgg tttacgtccg tcagcggaag caccccggcc tggcagacgg
     1141 ttcggaactg gccggtggcc caggggcggt ttttcaccac ggccgaggtc gagcaggtgg
     1201 cgaaggtcgc cgtcctcggc agcacggtcg cccagaacct cttccccgcc ggcggcgccg
     1261 tcggatcgac ggtggtgatc aagaacgtcc ccgtgcacgt gatcggggtc atggcctcga
     1321 aggggcagtc ggggttcggg cgggatcagg acgatttcgc gatggtgccc atcaccacgc
     1381 tgcaggagcg cctgaccggg gtcaccttca accggtcggt gtcggccatc ctgatctccg
     1441 cgacgtcgcc ggaaaccgtc cagggcgtca tcgacgagac ggagcggttg ctgcggatcc
     1501 ggcaccatct cacgcccggc cagcccgacg acttctccgt gcgaaacatc tccgacatcc
     1561 agcaggtgcg gatcgccacg tcgcagaccc aggcgctgct cctcgccgcc accgcggtcg
     1621 tctcgctcgt ggtggggggc atcggcatca tgaacatcat gctggtgtcc gtaaccgagc
     1681 gcacccgaga gatcggcctc cgcatggccg tgggcgccaa gggccgtgat atcctcctcc
     1741 agttcctcgt cgaggcattg acactcgcct ccatcggcgg cctcatcggg atcgcgatgg
     1801 gcctcgccac cgcccagggc gcgtcgacgt tggggcactg gccggtcctc gtctcgccgt
     1861 tctcgatgat tctggggttc gcctccgcgg ccctggtcgg gagcgtcttc ggattctatc
     1921 cggcgcagcg ggccgccgcg ctggatccga tcgtcgcgct ccggtacgag taggattcac
     1981 ttcacggcag gggcggcctc cggagcgaaa cgacaagcgc agccatgagc g
//
LOCUS       DASQGH010000369         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32838, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000369 DASQGH010000000
VERSION     DASQGH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32838"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1340)
                     /locus_tag="VEW91_07195"
                     /pseudo
     CDS             complement(148..1340)
                     /locus_tag="VEW91_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558251.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          192 a          628 c          480 g          207 t
ORIGIN      
        1 ggtccggggc ggagccccgg cgggtgcggg cggagcccgc ctgggtaatg agcggcggcc
       61 atggccgcct ccttctgctg gccggcgggg cctgctgccc ggcccgcggg cggggacccg
      121 gcccagcgtg ccgtcgcgcc aggcctgctc gtattcggcc ggcgggcggc cgccggtgcg
      181 ccgcatgacc cgccgggtgt tgtaccagcc ggcccattcg gcggtggcgg cctccacctg
      241 ggcgggggtt tcccacggcc ccaggtgatc gatgacctcg gtcttgtaca gcccgacgat
      301 gctctcggcc agcgcgttgt cgtaggagtc cccggccgtc ccgaccgacg gcgcgatccc
      361 cgccttcgcc agttcggcgc cgtagcggat cgacaggtac tcgctgccgg cgtcgctgtg
      421 gtgaatcagg cccgccactt tcaccccctg ccgggcgcgg taggtgaccg ccatcaccag
      481 cgcatccagc accagctccg tgcggtggtg cgccgcggtg cgccacccgg cgatcagccg
      541 ggagaacgcg tcgatcacca gcgcggtgta ggcggtgccc gcccaggtgg gcacgaaggt
      601 gaagtccacc acccacagcc ggtccggggc gccgggcgcg aactcccggt tcaccaggtc
      661 ctccggccgg tcctgcgacg ggtccgggac cgtggtccgc agcttcttcc acccgccccg
      721 gcgcaccccg cgcagcccca gcttgcgcat cagccgctcg acggtgcacc gggccgccgc
      781 gatgccctcg cggttcagct gggcccatac cttccacgcg ccggaccgct cgccgctttc
      841 cttatagacc cgcaggatct ccttctccag cgccgcgtcg cgcaccgccc gcgccgacag
      901 cggccgcccg cgcgccgcgt aataccccga cggcgagata ggcacgcccg cgccgcgcag
      961 aacccggcag accggcccgg ccccgaactc ctcccggtag gcctccacgt aggccaccgc
     1021 cagggttacc ggtggtcgag ctcggccgcg aaaaaagcac tcgccgtgcg caagatcgcg
     1081 ttcacccggc gcagctcagc gttctcccgc tccagcgccc ggatccgcgc cgcatccgcc
     1141 gacgcggtcc ccggccgcag cccccgctcg acctgcgcct gcttagccca gccgcgcagc
     1201 gtgtccgcgt tgatccccag ctcggcaccc accttccggc aggcagccgt cacgctggcc
     1261 tcctggtcct tgaccaggtc cagcaccagc cggatcgccc ggtcacgcag ctcgtccgga
     1321 tacttccgtg ctgctcccat cgtcccactc tcccgtggat tgggagcctc cacaaaaccc
     1381 ggggcgattc aggcccccgc ccccagcggc gccctcatcc tcgccgagct cagcgacatc
     1441 gccgtcacca ccatcaccgc cagcggccgg acaaccgtca tcccgcccga gctcaccgca
     1501 ctccagc
//
LOCUS       DASQGH010000370         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30005, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000370 DASQGH010000000
VERSION     DASQGH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30005"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEW91_07200"
     CDS             <1..252
                     /locus_tag="VEW91_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HYM91403.1"
                     /translation="GDREALLVGWLNELLYLLDTRRFLPHHSRITDLSDTNLAADLVG
                     DTVDPSRHAVRRMIKAATYHGLSVRKTNGLWEARVILDL"
     gene            315..591
                     /locus_tag="VEW91_07205"
                     /pseudo
     CDS             315..591
                     /locus_tag="VEW91_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904591.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(678..1121)
                     /locus_tag="VEW91_07210"
     CDS             complement(678..1121)
                     /locus_tag="VEW91_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYM91404.1"
                     /translation="MLRALCAHLGVQSAERSGNGGPESFDVDGGPRYTGVVSRFEHGW
                     GFIECEAMNRNFFVHYSDIEGTGMRLLEPGEVVSFELAPGKDGRPKAVRVRRQAASRP
                     APRPVRLPGLPRAEANNGAPEGKPEEVETGLEGSDLPGLESPSER"
BASE COUNT          261 a          514 c          489 g          256 t
ORIGIN      
        1 ggggaccggg aggcgctgct ggtcggctgg ctcaacgagc tcctctacct gctggacacc
       61 cggcggttcc tcccccacca cagccggatc accgacctga gcgacacgaa ccttgccgca
      121 gatctcgtag gcgacactgt cgacccaagc cgccatgccg tacggagaat gatcaaggcg
      181 gctacgtatc acgggctctc cgtccgcaag acgaacggct tgtgggaagc ccgggtcatc
      241 ctggacctct aggagcgagg tcggtgtccc caggggctca ggtcctccgc cagcgggacg
      301 cgtaccgctg agagatcccg gagacgtaca agccggggat tcgtgtgccc gggctcatct
      361 acgcggacga ggccctgatc aagcagatcg aggaagacca ggcgatcgag caggtcgcga
      421 atgcagccac cctgccgggt gtcggccgct gggcgatcgc gatgccggac attcactggg
      481 gctacggctt cccgatcggc ggcgtggcgg cgatgcgcct ggacgacggt gtcgtctccc
      541 cgggcggctg tcggctacga catcaactgc ggggtgcggc tgctggcgac gcgacccacc
      601 gagtagcgac ggcgcggggc tgacccgcac cgtcgcccga cttcagccgc tggccgtggt
      661 caagggataa gctgcggtta ccgctcggac gggctctcca gccctggaag atcgctccct
      721 tccagaccgg tctcgacttc ctcgggcttc ccctcgggag ctccgttgtt ggcttcagcc
      781 cgcggaaggc cgggcaacct aaccggccgc ggcgcgggac ggctggccgc ctggcggcgc
      841 acacggactg cctttggccg gccgtccttg ccgggggcca gttcgaacga gaccacctcg
      901 ccgggctcca gcaaccgcat tccggtgcct tcgatgtcgc tatagtgcac aaagaagttg
      961 cggttcatcg cctcgcactc gatgaagccc cagccgtgct cgaacctcga cacgacgccg
     1021 gtgtaccggg ggcctccgtc cacgtcgaag ctctccggcc ctccgttccc cgaccgctcg
     1081 gctgattgga cacccaggtg cgcgcaaagg gcgcggagca tcgattctat ccggtccaat
     1141 tgcgaagaca tatccaaagc cctccaaccg tccgtgcggg ccgcaaaacg accctgagca
     1201 ctcgtaacct acgggggcag gcacgggttg gttcaggatt cgcggaaact cgcccggact
     1261 agattttcca cctggcgtta gtgtacaaac ttttacctat ggcggtcaac gctcagggca
     1321 cacatgggga ggcgccacac tgtggggaag tacgcggagg tcttgaagct agttggacga
     1381 ctctttcccc tggctggggg ccggaaccct tccgttggcg agggtgagaa ccagacgcgt
     1441 atccgtcgat tccaccacgt ccaccgggaa gcccgttctc aggtcatcgc taatgcccgc
     1501 gagggtgccc tcctccgtca
//
LOCUS       DASQGH010000371         3962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4485, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000371 DASQGH010000000
VERSION     DASQGH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4485"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VEW91_07215"
     CDS             <1..994
                     /locus_tag="VEW91_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYM91405.1"
                     /translation="PAFVLFNNLAPQPGDRLWAPADWAWIGGLFNVLWAGWHYGIPVV
                     AFRQPKFDPERAFWILERHAIRNAFLFPTALKLMRQIHAHGFREGVRLRSVYSAGEPV
                     GEELIGWGREVLGVTINEFFGQTEMNLVAGNSAELLPVKPGSFGKPYPGHRVDVLGED
                     GHPVPPGTLGEVAVRRPDPVMFLEYWNHPDATQRKFVGDWAMMGDLAIKDEDGYLWFK
                     ARKDDMIKSGAYRIGPGEVEECLLRHPAVAMAAVVGVPDPERGQAVKAFIRLAAGGSP
                     PSGLAAELQQFVKDRVGLHAYPRSIEFVDELPLTTTGKLQRFVLRDRTRPSGGD"
     gene            complement(1005..1673)
                     /locus_tag="VEW91_07220"
     CDS             complement(1005..1673)
                     /locus_tag="VEW91_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HYM91406.1"
                     /translation="MTLSGRRILVTSGPTRAPLDAVRFLTNKSTGRLGALIAEAAVQA
                     GADVTFVYGRGSDTPAVRGGHQDHLRLLSVDTVDDLIAVFKKELPTGYDAVVHAMAVL
                     DFEPAEVREEKTSSDLSEWVVRLTPTPKASRLVKTLAPKTCFVGFKLEVGKERDELLE
                     ISSAWAAKNGADLVVANDLRDIEGGLHIGYLIRNGMVEAIAEGKEAIARVVVDFLGRS
                     LKDR"
     gene            complement(1670..2833)
                     /gene="queG"
                     /locus_tag="VEW91_07225"
     CDS             complement(1670..2833)
                     /gene="queG"
                     /locus_tag="VEW91_07225"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HYM91407.1"
                     /translation="MTVDLIRLAREVREEARAAGFDLCGITTSAPFEREGRALADWVD
                     RGYHGEMGYMARNAPRSGDPQKVVPAARALVVVGLYYGGAAERTAGDPAPDGAAGPRG
                     RISRYAQGRDYHQVMEPGLRRLAMYLLARGARVARYYVDTGPVIDRAAARRAGLGWYG
                     KNTLIITRSGFGSWVFFGEILTDLPLPADAPDEDDCGRCRLCLDACPTGAIVAPYMVD
                     ARRCISYLTIEHRGSIPRDLRPAIGDHIFGCDICQAVCPHNARTATQRPPAFAPQAGA
                     GARPALLPLLNITEEEFRTHFNGTPVKRARRTGVRRNVAVALGNLKDPSAVPGLLLAL
                     ADEEDPLVRGHAAWALGRIGATAAREGLRKRLSHEQDAWVREEISLALGAPPA"
     gene            2927..3547
                     /locus_tag="VEW91_07230"
     CDS             2927..3547
                     /locus_tag="VEW91_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91408.1"
                     /translation="MEPPSVPLAVTDIARGFGRRQVLRAVTFAARRGEMVGIVGENGA
                     GKSTLLRILAGLLPPDRGRVRVRGRIGYCPQDPQVHAGLTVDHNLEWFRAAYRLRDAH
                     RAEEMMSLLGFAQHRGTLVGELSGGTRQKLNLVLALMHDPDVLLLDEPYQGFDWETYQ
                     RFWGIAEDSRRAGRVIVVISHLFYERHRFDTLLRLRDGVLAPEGSA"
     gene            3544..>3962
                     /locus_tag="VEW91_07235"
     CDS             3544..>3962
                     /locus_tag="VEW91_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91409.1"
                     /translation="MIEQWWIALRLSLIEQAHNRFAMLLLALYLPAWYGVIYGLTDEA
                     SIGFQLRAFHVFVATNQHRLGLLTGMLNATTLIMGFVALSAVRRSALVDQRLVLCGYS
                     RGALILGRLVALAVACALVSLYAVFVLALFTPPQHPGV"
BASE COUNT          574 a         1348 c         1388 g          652 t
ORIGIN      
        1 gccggcgttc gtcctcttca acaacctcgc gcctcagccg ggagatcgtc tctgggctcc
       61 ggcggactgg gcctggatcg gcggtctctt caacgtgctg tgggccgggt ggcactatgg
      121 catccccgtg gtcgcctttc gccagcccaa gttcgatccc gagcgcgcct tctggatcct
      181 ggagcgtcat gcgatccgta acgccttcct gttcccgacg gccttgaagc tgatgcggca
      241 aatccacgcg cacggcttcc gcgagggggt gcgcctgcgg tccgtgtaca gcgcgggaga
      301 gccggttgga gaggagttga tcgggtgggg gcgagaggtc ctgggggtga cgatcaacga
      361 gttcttcggg caaacggaga tgaacctggt ggccggcaac tccgcggaac tcctgcccgt
      421 gaagccgggg agcttcggga aaccctatcc gggccaccgc gtggacgtgc tcggcgagga
      481 cgggcacccg gtgcccccgg ggaccctcgg cgaggtggcc gtccgccggc ccgacccggt
      541 gatgttcctg gagtactgga accatcctga cgccacgcag cggaagtttg tcggcgactg
      601 ggcgatgatg ggggatctgg cgatcaagga cgaggacggc tatctctggt tcaaggcgcg
      661 caaagacgac atgatcaaga gcggagccta ccgcatcggg ccgggcgagg tggaggagtg
      721 tttgctgcgc catccggccg tggcgatggc ggcggtggtc ggcgtccccg accccgagcg
      781 gggccaggcc gtcaaggcgt ttatccggct ggccgccggc ggatcacccc cgtcggggct
      841 ggcggccgag ctccagcagt ttgtgaagga ccgggtggga ctccacgctt acccgcgcag
      901 tatcgagttc gtcgacgagt tgccgttgac cacgacgggc aagctgcagc ggtttgtctt
      961 gcgcgaccgc acgagaccat cgggtggaga ttgatgcccg cggtctaccg atccttgagg
     1021 ctccggccga ggaagtcgac caccacccgg gcaatcgcct ccttgccctc ggcgatggcc
     1081 tccaccatcc cgttccggat gagatagccg atatgcaatc ccccctcgat gtcccggagg
     1141 tcattggcca cgaccaggtc cgcgccgttc ttggccgccc atgcgcttga gatctcgagc
     1201 aactcgtccc gctccttgcc gacctcgagc ttgaacccga cgaaacaggt cttgggcgcg
     1261 agggtcttga ccaggcggga ggccttgggc gtcggggtca ggcggacgac ccactccgac
     1321 agatcgctgc tggtcttctc ctcccggacc tcggccggtt cgaagtcgag gaccgccatg
     1381 gcgtgcacga ccgcatcgta gccggtgggg agctctttct tgaagaccgc gatcaggtca
     1441 tccaccgtgt cgacggatag cagccgcagg tggtcctggt ggccccccct gaccgccggg
     1501 gtgtcgctcc cgcggccgta gacgaacgtc acatcggcgc cggcctgcac cgccgcttca
     1561 gcgatcagcg ccccgagcct gcccgtggac ttgttcgtga gaaaccgcac agcatcgagg
     1621 ggggcacggg tgggcccaga ggtgacgagg atgcggcggc cggagagggt cacgcggggg
     1681 gcgctcccag cgcaagcgag atctcttccc gcacccaggc gtcctgctcg tgcgaaagcc
     1741 gcttccgaag cccctcccgt gccgcggtgg ctccgatccg gccgagggcc caggccgcgt
     1801 ggccgcgcac cagcggatct tcctcgtcgg ccagcgcgag gagcagcccc ggcaccgccg
     1861 agggatcttt gaggttgccc agtgcgaccg cgacgttgcg gcgcaccccc gtgcgtctcg
     1921 ctcgcttgac cggcgtgccg ttgaaatggg tgcggaactc ctcctcggtg atgttcagca
     1981 ggggcagcag cgccgggcgg gcgccggcgc cggcctgcgg ggcaaatgcc gggggtcgct
     2041 gggttgcggt tctagcgttg tgcgggcaca ccgcctggca gatgtcgcag ccgaaaatgt
     2101 ggtcgccgat cgcgggccgg aggtcgcgcg ggatcgaacc gcggtgctcg atcgtcagat
     2161 aagagatgca ccggcgggcg tcgaccatgt agggcgcgac gattgcgccg gtggggcagg
     2221 cgtcgaggca cagccggcag cggccgcaat cgtcttcatc gggagcatcc gccggcaggg
     2281 ggaggtcggt gaggatttcc ccaaagaaca cccaggagcc gaacccgctg cgggtgatga
     2341 tcagcgtgtt cttgccgtac cagccgagtc ccgcgcgcct cgctgccgcc cgatcgatca
     2401 ccggtccggt gtcgacgtag tagcgggcca cgcgcgcgcc ccgggccagc aggtacatcg
     2461 ccagccggcg gaggccgggc tccatgacct ggtgatagtc gcgaccttgc gcgtaccggc
     2521 tgatccggcc ccgcgggccc gccgcgccgt ccggggcggg gtcaccggcc gtacgctccg
     2581 ccgcgccgcc gtagtacagg ccgaccacga cgagcgcacg ggcggcggga accaccttct
     2641 gcggatcccc ggacctcgga gcgtttcggg ccatgtaccc catctcgccg tggtagccgc
     2701 ggtccaccca gtcggcgagg gcccggccct cgcgctcaaa cggcgcgctc gtggtgatgc
     2761 cgcacagatc gaatccggcg gcgcgcgcct cttccctaac ctcgcgcgcc agcctgatca
     2821 aatccacggt catgggcggc tccatcggcg ctcccgcttt cgagtatagc acccgcgaag
     2881 acgcctgccg gaggagttct ttgggcacgc tggaacctgt cgtggcatgg aacccccctc
     2941 cgtcccactc gccgtcacgg acatcgccag ggggttcggc cgccgtcagg tgcttcgcgc
     3001 cgtcacgttc gccgcccgcc ggggggagat ggtggggatc gtgggggaga acggggcggg
     3061 aaaatccacc ctgcttcgca tcctcgccgg gctgctgccc ccggaccgcg gccgggtgcg
     3121 ggtccgcggc cggatcggct actgccccca ggacccgcag gtgcacgccg gactgaccgt
     3181 cgatcacaac ctggaatggt ttcgcgccgc gtaccgcctc cgggatgcgc accgggccga
     3241 agagatgatg agcctgctgg ggttcgcgca gcatcgcggg acgctcgtcg gtgagctgag
     3301 cggggggacc agacagaagc tcaacctggt gctcgcgctg atgcacgatc ccgacgtcct
     3361 cctcctcgat gagccctacc aggggttcga ctgggagacg taccagcggt tctgggggat
     3421 cgccgaggac tcccggcgcg ccggccgggt catcgtcgtc atctcgcacc tgttctacga
     3481 gcggcaccgc ttcgacacgc tcctccggct gcgcgatggc gtgctcgccc cggaggggag
     3541 cgcatgatcg aacagtggtg gatcgccctc cggctgagcc tgatcgagca ggcgcacaac
     3601 cgcttcgcga tgctcctcct ggcgctctac ctcccggcgt ggtacggggt catctacggg
     3661 ctcacggacg aggcctcgat cgggttccag ctgcgcgcct tccatgtttt cgtggcgacc
     3721 aaccagcacc gcctcgggct actcaccggg atgctgaacg cgacgaccct gatcatgggg
     3781 ttcgtcgcgc tgagcgcggt caggcggtcg gccctcgtgg accagcggct cgtgctgtgc
     3841 ggctactcgc ggggcgcgct catcctggga cgcctggtgg cccttgcggt ggcgtgtgcg
     3901 ctcgtctccc tgtacgcggt gttcgtgctc gcactgttca cgcctcccca acatcccggt
     3961 gt
//
LOCUS       DASQGH010000372         5384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18635, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000372 DASQGH010000000
VERSION     DASQGH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18635"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..400)
                     /locus_tag="VEW91_07240"
     tRNA            complement(324..400)
                     /locus_tag="VEW91_07240"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Ile,seq:gat)
     gene            complement(473..1984)
                     /locus_tag="VEW91_07245"
     CDS             complement(473..1984)
                     /locus_tag="VEW91_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM91410.1"
                     /translation="MPRLPGDARSAAGPHRSAPWEGRHLPPGVYRTCLLLIVLVGAVL
                     RLAHLARPFNGIGATAWNEGHYALIALNFDRYGLWSQHNELGADYTFAPGVPWLVWLA
                     FKAFGPTEWGARLPIALFGIAALPLVAALARRLLDSEQIAIVAAGLVAVLPETVYFSQ
                     NVQLDTPSTCCALAGAVALLRYRDTGRRGGVIAAGVWVTLAIWFKFTTALLYPAYLAL
                     WWPARPRRPLVAAGAAAAFVALTVLPSAAWIAYARLTNQMLVSFYQRGWDLRLAAQVL
                     IELPLMVGTHFFPLGFVLLVLGIPVAWRWRARLRGLGIWCGAWVVPYLAFPHSALNNR
                     YYDLPATYLLTIPAAFGLWMPLARRRTGPALGRAVLVALGLFMAVVAAYDLWDPTTDR
                     LARTMIAHPPPLDPTPFYSAKVVATLPRKRTVVDAPQTMFYAGGDPAWVSIVGGGADV
                     REAIDGETFDYILLNDYWHAQGPYYPVDDALRARLRLHHYVQIAPAAWARAAR"
     gene            complement(1999..3216)
                     /gene="tyrS"
                     /locus_tag="VEW91_07250"
     CDS             complement(1999..3216)
                     /gene="tyrS"
                     /locus_tag="VEW91_07250"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HYM91411.1"
                     /translation="MTTLSPSAQLERLKRGAVEVISEDELLAKLALGRPLRVKLGLDP
                     TAPDLHIGNAIVLQKLRQFQDLGHEAIVVIGDFTGLIGDPSGKSETRRALSPQEIEQN
                     AQTYRDQYRLILDPARTRVVFNSQWLGAMTFYDVVKLASRTTVHRILERDDFAKRYAD
                     RLPIHLHELLYPLCQAYDSVAIAADVELGGTDQKFNNLMGRELQRELGQEPQVVLLTP
                     LLPGLDGVQKMSKSLGNAIGITEPPNEMYGKVMSVSDDLMIPYFEYCTLVPLEEVRGI
                     AAALAAGGVHPRDAKKRLAREITGRYHGEAAARRAEAEFERVFAARERPAEIPDVELS
                     RDRLRGGAIRLVRLLAELGLADSNSEARRLISQGGVSLDGARLTRDVDVTIQDGALIQ
                     VGRRRFARIRLTE"
     gene            complement(3233..4144)
                     /locus_tag="VEW91_07255"
     CDS             complement(3233..4144)
                     /locus_tag="VEW91_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYM91412.1"
                     /translation="MHTHPAPADPHRPFAVALGITLVLLAVEAVGGWLTGSLALLADA
                     GHLLADIGTLGIGFIGSWIASRPATAKMSYGYRRAEILAAATNACALWAVAGAILYEA
                     VQRLRLLHPVSAPGMLAVAVLGLAGNLASSAVLARDRGNLSLRVALTHTLADAAAAVG
                     TIAASFVILVTRWVQADAVVSIGIAALILAGSWPPLREAVQILMEGAPTRLSLTEVEE
                     AMRGVPGVTGVHDLHIWSLTAGVEAVSGHVLVADAEVSQRVLGDLCRLLRDRYGLGHA
                     TLQLETEASTDPSHPNCAPEPRAGPGR"
     gene            complement(4147..>5384)
                     /locus_tag="VEW91_07260"
     CDS             complement(4147..>5384)
                     /locus_tag="VEW91_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYM91413.1"
                     /translation="QSIEQNLSTFSSHLGAIVEILRALGEHPLGGGRALVLLDEVGAG
                     TDPTEGVALARALIETLHNLGACTAVTTHYNELKALPYTHPGMENASVEFDESTLRPT
                     FRLLIGTPGRSNAFAIAERLGLDPRIVAQAQSAVSRRDADLATVFQSVEAERDAFARE
                     REALARDRAACDRLRAQVEAEARRLEEDRREFFERARSEVSALVRRGRQELDTLLAAL
                     RERPSGEAAQRLRLHLQDLARASEAYAARGQPPPAGSPPEDLQSGERVFVVSLNQRGI
                     VQIPPDTRGEVEVQAGTLKLRVPVADLRRLPPGEAGGPAEPLQAEPGLTKALAISPTL
                     DLRGLRAEDAVEQLDKYLDDATLAGLARVTVIHGKGTGALRQAVRDHLSRHPEVAAFR
                     LGAESEGGSGATIVELQRH"
BASE COUNT          840 a         1890 c         1899 g          755 t
ORIGIN      
        1 ctccagcctg cttaagaatg gacgtggccg cgtaatgcct tagagcatgc ggccccacct
       61 tccgtggcag gccagcgcgg acggatagcc gttggagtat gtgaccgcaa ccgtcgtcca
      121 accagagggc tcaggctcaa cagcggcgag gaacgggcga agcacctcac gatagagttg
      181 aactgtccga gcggtacagc tagacacgct cttggatagc agaaacaccc ctgggacgtc
      241 ttctgcggtt cgaggggtga gcataccctt tcgggcgtct ttgacaagcc tggacaacgc
      301 tgtaggcctt gatttgcaag taatggtggg ccctcgtgga ctcgaaccac ggacctcacc
      361 cttatcaggg gtgcgctctc accacctgag ctaagggccc gctgcaccca catactatcc
      421 gaagcgaggt cccggcgtca atcaacgcgc acgatcgatc tgcggtgcgc gcctaccgcg
      481 cagcacgcgc ccacgccgcc ggcgcgatct gcacgtagtg gtgaaggcgc aatcgcgccc
      541 gcagggcgtc gtcgaccggg taatagggtc cctgggcgtg ccagtaatcg ttgagcagga
      601 tgtagtcgaa cgtttctccg tcgatcgcct ccctcacatc cgcccccccg ccgacgatgg
      661 agacccacgc gggatctccc ccggcgtaga acatcgtctg cggggcgtcc accaccgtgc
      721 gtttccgggg cagggtggcg acgaccttgg cggagtagaa cggcgtggga tcgagcgggg
      781 gaggatgggc gatcatcgtc cgggccaggc ggtcggtcgt gggatcccac agatcgtagg
      841 ccgccacgac cgccatgaac agcccgaggg ccaccagcac cgcgcgcccc agcgcgggcc
      901 ccgtgcgccg ccgggcgagc ggcatccaca gcccgaacgc ggccggaatc gtgaggaggt
      961 acgtggccgg gaggtcatag tagcggttgt tgagggcgga atggggaaac gccaggtacg
     1021 gcaccaccca cgcgccgcac cagatcccca ggccccgcag gcgggcgcgc cagcgccacg
     1081 ccaccgggat cccgagcacc agcagcacga acccgagcgg gaagaagtgc gtgccgacca
     1141 tgagcggcag ctcgatgagc acctgggcgg ccaggcggag gtcccacccc cgctggtaga
     1201 agctcaccag catctgattc gtgagtcggg cataggcgat ccacgcagcg ctggggagga
     1261 ccgtcagggc gacgaacgcc gccgccgcgc ccgcggcaac caggggccgg cggggccggg
     1321 ccggccacca caaggcaagg tacgcgggat agaggagcgc ggtcgtgaac ttgaaccaga
     1381 tcgcgagggt gacccagacc ccggcggcga tgaccccgcc ccggcgcccc gtgtcgcggt
     1441 accggaggag ggccacggcc ccggcgaggg cgcagcaggt gctcggggtg tcgagctgga
     1501 cgttctgcga gaagtacacg gtctccggca gcaccgcgac gaggcccgct gccacaatgg
     1561 cgatctgctc gctgtcgagg agccgccgcg caagcgcggc aaccaacggg agggcggcga
     1621 tgccaaacag ggcgatcggg aggcgtgcac cccattcggt cggcccaaac gccttgaacg
     1681 ccagccagac gagccagggc acccccggcg cgaacgtata gtccgcgccg agctcgttgt
     1741 gctgggacca caggccgtac cggtcgaagt tcaacgcaat cagggcgtag tgcccctcgt
     1801 tccacgcggt ggccccgatg ccgttgaacg gacgggcgag gtgcgcgagg cgcagcaccg
     1861 cccccaccag gacgatcagg agcaggcagg tccggtagac gccgggaggc aggtgccgtc
     1921 cttcccacgg cgccgaacga tgcggccccg cggcggatcg cgcatcgccg gggagtcggg
     1981 gcacggaccc gggcgatcct actcggtgag ccggatccgg gcaaaccggc ggcggcccac
     2041 ctggatcaac gccccgtcct ggatggtgac gtcgacatcc cgggtcaggc gcgcgccgtc
     2101 gaggctaacc ccgccctggc tgatcagccg ccgggcctcg ctgttcgagt cggccagccc
     2161 gagctcggcg aggaggcgga cgaggcggat cgcgcccccc cggagccggt cgcgggagag
     2221 ctccacgtcg ggaatctccg ccgggcgctc gcgcgcggcg aagacccgtt caaactcggc
     2281 ttccgcccgg cgcgccgcgg cctctccgtg gtaccgcccg gtgatctccc gggcgaggcg
     2341 cttcttggcg tcgcgggggt gcaccccgcc cgccgccagg gcggccgcga tcccgcggac
     2401 ctcctcgagc ggcaccaggg tgcagtattc gaaatagggg atcatgaggt cgtcggagac
     2461 cgacatgacc ttcccgtaca tctcgttcgg gggctcggtg atcccgatgg cgttcccgag
     2521 gctcttgctc atcttctgca ccccgtcgag cccggggagc agcggggtga gcagcacgac
     2581 ctggggctcc tgtccaagtt cccgctggag ctcccgcccc atgagattgt tgaatttctg
     2641 atcggtgccg ccgagctcga cgtcggcggc gatcgcgaca gagtcgtagg cctggcagag
     2701 cgggtagagc agttcgtgca gatggatcgg gagccgatcg gcgtagcgct tggcaaaatc
     2761 gtcgcgctcc aggatgcggt gcaccgtggt gcggctggcc agtttgacga cgtcgtagaa
     2821 cgtcatcgcc cccagccact ggctgttgaa caccacccgg gtccgggccg gatcgagaat
     2881 caagcggtac tggtcccggt acgtctgcgc gttctgctcg atctcctggg gcgacagcgc
     2941 gcggcgggtc tcggacttgc ccgatggatc gccgatgagg ccggtgaagt caccgatgac
     3001 gacgatcgcc tcgtggccca ggtcctggaa ctgccgcagc ttctggagca cgatggcgtt
     3061 gccgatgtgg aggtcgggcg cggtcgggtc cagtcccagc ttgacccgca gcgggcgccc
     3121 gagcgcaagc ttggccagca actcgtcttc ggagatgacc tcgacggccc cgcgcttgag
     3181 cctctcgagt tgcgcgctgg ggctcaacgt ggtcatgcgt gtgagtatag catcagcggc
     3241 ccggccctgc ccggggctcc ggcgcgcagt tcgggtggct ggggtcggtc gaggcttcgg
     3301 tctccagctg cagcgtggcg tgccccagcc cgtaccggtc gcgcagcagc cggcacagat
     3361 cccccagcac ccgctggctg acctcggcat cggccacgag cacgtggccg ctcaccgcct
     3421 cgacgccggc ggtcagcgac cagatgtgga ggtcgtgcac gccggtcacc cccggcaccc
     3481 cccgcatcgc ctcttccacc tccgtcaacg agaggcgcgt cggcgcgcct tccatgagga
     3541 tctgcaccgc ctcgcgcagc ggcggccacg atccggccag gatcagcgcg gcaatgccga
     3601 tgctgaccac cgcgtccgcc tgcacccagc gcgtgacgag gatcacgaag ctggcggcga
     3661 tcgtgcccac ggcggcggcc gcatccgcca gggtgtgggt cagcgcgacc cggaggctga
     3721 ggttgccgcg gtcgcgcgcc agcaccgcgc tgctcgccag gttcccggcc aatccgagga
     3781 ccgcgacggc gagcatcccg ggcgccgaca cggggtgcag cagccggagg cgctggaccg
     3841 cttcgtagag aatcgcgccg gcgacggccc acagcgcgca ggcgttggtc gccgccgcca
     3901 ggatctctgc ccggcggtag ccgtaggaca tcttcgccgt ggccggacgg gacgcgatcc
     3961 agctgccgat gaacccgatc cccagggtac cgatatccgc gagcaggtgc ccggcatcgg
     4021 ccaggagggc caggctgccc gtcagccatc cccccacggc ttcgacggcc agcagcacga
     4081 gggtgatgcc gagcgccacc gcgaacggcc ggtgcggatc cgccggggcg gggtgcgtgt
     4141 gcatcttcaa tggcgctgga gctccacgat cgtggccccg ctccctccct ccgattctgc
     4201 gccgagccgg aacgcagcga cctcggggtg ccgggagaga tgatcgcgca ccgcctggcg
     4261 cagcgctcca gtacccttgc cgtggatcac ggtgacccgc gcgagccccg cgagcgttgc
     4321 gtcatcgagg tatttgtcca gttgctcgac ggcatcctcc gcccggagac cccgcaggtc
     4381 cagggtcggc gagatcgcca gcgctttcgt gaggcccggt tcggcctgga gcggttctgc
     4441 ggggccgccg gcctcgcccg gagggagccg gcggagatcg gcgaccggca cccgcagctt
     4501 cagcgtgccg gcctgcactt cgacctcgcc gcgggtgtcc ggagggatct gcacgatccc
     4561 gcgctggttg agcgacacca cgaagacgcg ctccccactc tggaggtcct ccggcgggga
     4621 tcccgccggt gggggctgcc cccgcgccgc atacgcctcg gaggcgcgcg cgaggtcttg
     4681 gaggtgcagc cggaggcgct gggcggcctc cccggacggg cgctcgcgga gggcggcgag
     4741 cagggtgtcg agctcctgcc gcccccgccg caccagcgca ctcacctccg agcgcgcgcg
     4801 ctcaaagaac tcccgccggt cctcctcgag gcgccgggcc tccgcctcca cctgcgcccg
     4861 caaccggtca catgcggccc ggtcccgcgc gagcgcctcg cgctcgcggg cgaacgcgtc
     4921 gcgttcggct tccaccgatt ggaacacggt cgcgaggtcg gcgtcccggc gcgagacggc
     4981 gctttgcgcc tgggccacga tccgggggtc cagcccaagc cgctcggcga tcgcaaaggc
     5041 gttgctgcgc cccggggtcc cgatgagcag ccgaaacgtg ggacgcaggg tcgactcgtc
     5101 gaactcgacg gaggcgtttt ccatgcccgg gtgggtgtac ggcagggcct tcaactcgtt
     5161 gtagtgcgtg gtcaccgcgg tgcacgcgcc caggttgtgc agcgtctcga tcagcgctcg
     5221 ggccagcgcc acgccttcgg tgggatccgt ccccgccccc acctcgtcga gcaggacgag
     5281 ggcccggccc cccccgaggg gatgctcccc aagcgcgcgg aggatctcca cgatcgcccc
     5341 gaggtgcgag gagaacgtcg agaggttctg ctcgatgctc tgtt
//
LOCUS       DASQGH010000373         5093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_15760, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000373 DASQGH010000000
VERSION     DASQGH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15760"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VEW91_07265"
     CDS             <1..1181
                     /locus_tag="VEW91_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYM91414.1"
                     /translation="TSAEGAALVNGTSGHALDYDDVSISVRGHPSVVVLPAALAVAQS
                     IRASGRALLEAYVAGVEVTAKLGRAMGPAHYQAGWHATSTLGTLGSAAAAGRLLGLDA
                     ARLAHALAIAVSEASGSRQNFGTMIKPFHTGHAARCGVHAARLAQKGLTGDTTAIEGP
                     VGFFAMFAFGEARPADLVRQLGAPFDLVAPGFSVKKYPCCFATHRAADGVLDLAGAHG
                     IRADDVDAVRVTVPVGGGQPLIHDRPQTGLQGKFSMQYVVAAALLDRRLGFDSFSDAA
                     VLRPAAQALLRRVTVVEDPAIADAANPMEQGYVTVEVRTRTGRRFTGRVDHPRGSPEL
                     PLGRAELAAKFRDCAARALDPASVDRAVTLLDAIESVQDVNTLVDSLAPGRTSTATVR
                     "
     gene            1178..1306
                     /locus_tag="VEW91_07270"
     CDS             1178..1306
                     /locus_tag="VEW91_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91415.1"
                     /translation="MTTGLPRVLAELGFPPQEVEALLDSQTCARAQRASATRPVDG"
     gene            1369..2529
                     /locus_tag="VEW91_07275"
     CDS             1369..2529
                     /locus_tag="VEW91_07275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009831085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM91416.1"
                     /translation="MDFRLTERQEQIRTEVRALCRRFPPEYWQRLDAERRYPDEFVGA
                     MTAAGWLAVLIPREYGGGGLGTLEAGLVLEEINRSGGNAQPCHAQIYTMGALLRHAGD
                     DHKRRYLPAIAKGELRLQAFAVTEPDAGTDTTSISTFAERRGDKYVVHGRKIFISRVQ
                     HSELMMLLARTTPLDQVKKRSHGLSLFLVDLRTAGSRLRVTPIKTMVNNETNELVLEG
                     VEIPAENRIGEEGLGFSYILSGLNVERILIASECIGDGTYFIERATRRATDRVVFGRP
                     IGQNQGVQFPIAKAYTALEAARLMRDKAATRFDRGEPCGAEANMAKYLASEAAWEAAN
                     VAMTTFGGYGMAVEYDIERKFRESRLHLVAPVSNNLILSFVGQHVLGLPRSF"
     gene            2538..3485
                     /locus_tag="VEW91_07280"
     CDS             2538..3485
                     /locus_tag="VEW91_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYM91417.1"
                     /translation="MRWPDAVTVHEVGPRDGFQMEKPFIPTEVKIRLIDALSHTGLRE
                     IQATSFVHPQAIPQLRDAEAVMAGIERVPGVAYTALVLNERGAERAVAARVDRIDIAV
                     SASDSHSLSNANMTTAQAMECAGRVAAMGRDAGTDVAIGFATALGCPFEGVLPYGRVE
                     ALVARAVEEFGLARVGIADTVGMANPALVWTTMDALRRRFPAASFSLHLHNTRGMGLA
                     NILAGCDAGVAHFDASVAGLGGCPYAPGATGNIATEDAVHMLEEMGIATRVRLDALLD
                     VARQVEEAVGHADSAVLRAGTSRMLLGARRGGQTKLGTP"
     gene            complement(3493..4407)
                     /locus_tag="VEW91_07285"
     CDS             complement(3493..4407)
                     /locus_tag="VEW91_07285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM91418.1"
                     /translation="MGDSSVPRAELQRVGFIGLGKMGTPMAHRLLAAGYRVIAHDVRT
                     EAVAEACNAGAASGDSPAAVAARADVVITMLPDARAVERVVYGARGLGEAMRAGQVLL
                     EMTSSHPRTTRRIAADLRPKGVHVLDCPVSGGVRGAADGTLCVMAGGPAEVVEACRPI
                     LERFGEVVHVGDAAGDGDTAKTINNLLSATTIWSVVEALTLGVKAGLPPDRLFAAVNR
                     STGRSYTTETKVSRYFLPRRFDAGFTVGQYVKDLDICLDLADELGVPMLLGSLVRQAW
                     TVAAQEGMADRDHTELVRLMERRAGLGS"
     gene            complement(4447..4545)
                     /locus_tag="VEW91_07290"
                     /pseudo
     CDS             complement(4447..4545)
                     /locus_tag="VEW91_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            4795..>5093
                     /locus_tag="VEW91_07295"
     CDS             4795..>5093
                     /locus_tag="VEW91_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91419.1"
                     /translation="MACVIAIAFLLAYAVPPTGAAMMMGADVKKELQTAMFHAVELAQ
                     RGNVVATSQLHVQHVINCLEGPNGANFKAAAGYPCQGMGNGIIPDLKDAVAAKVPG"
BASE COUNT          788 a         1771 c         1800 g          734 t
ORIGIN      
        1 gcacgtcggc ggagggcgcg gcgctggtga acggcacgag cggccacgcc ctcgattacg
       61 atgacgtcag catctccgtg cggggccacc cgagtgtcgt cgtgctgccg gccgcgctgg
      121 ccgtggccca gagcatccgt gcgtccgggc gcgcgctgct ggaagcgtac gtggcaggcg
      181 tcgaagtcac ggcgaaactg ggccgcgcga tggggccggc ccactaccag gccgggtggc
      241 acgccacctc caccctcggg acgctgggca gtgccgcggc cgcgggcagg ctgctcggac
      301 tggatgcggc ccgcctcgcg cacgccctgg cgatcgccgt ctccgaggcc tccggcagcc
      361 gccagaactt cgggacgatg atcaaaccct tccacacggg ccacgccgcc cgctgcgggg
      421 tgcatgcggc ccggctcgcc cagaaggggc tgaccggaga caccaccgcg atcgagggcc
      481 cggtcggctt cttcgccatg ttcgcgttcg gggaggcgcg ccccgcagac ctcgtccgcc
      541 aactcggcgc gcccttcgac ctcgtcgcgc cgggcttcag cgtgaagaag tatccgtgct
      601 gcttcgccac ccatcgggcg gccgacggcg tgctcgacct ggccggcgcg catgggatcc
      661 gcgcggacga cgtcgacgcc gtccgggtga ccgtcccggt cggcggcggc cagccgctca
      721 ttcacgaccg gccgcagacg gggctccagg gcaagttcag catgcagtac gtcgtcgccg
      781 cggccctcct cgaccggcgc ctggggttcg actccttctc cgacgcggcc gtgctgcgcc
      841 cggcagcgca ggcgctcctc cggcgggtga ccgtcgtcga ggatccggcg atagcggatg
      901 cggccaaccc gatggagcag gggtacgtca ccgtcgaggt ccgcaccagg accggccggc
      961 gcttcaccgg cagagtcgac catccgcggg ggtctccgga gctcccgctg gggcgcgcgg
     1021 agctcgccgc caagttccgc gactgcgccg cacgggccct tgatcccgcc tccgttgacc
     1081 gggccgtgac cctcctcgac gccatcgagt cggtgcagga cgtgaacacg ctcgtcgact
     1141 ccctggcccc cggccggacg tcgaccgcca cggtgcgatg accacgggcc tgccgcgggt
     1201 cctggccgaa ctaggctttc ccccccagga ggtcgaagcg ctgctggact cccagacgtg
     1261 tgcgagggcg cagcgcgcgt cggccacccg gccggtggac gggtaacggc cgctcacggg
     1321 gcggtcaccc caccgatccg ggcgacccac cggatcgagg aggcggcgat ggatttcagg
     1381 ctcaccgagc ggcaggagca gatccgcacc gaggtgcggg cgctgtgccg gcggtttccc
     1441 ccggagtact ggcaacgact cgatgcggag cgccgctacc ccgacgagtt cgtcggcgcg
     1501 atgacagcgg cggggtggct cgcggtgctc atcccccggg agtatggcgg cggcgggctc
     1561 ggcacgctcg aggccgggtt ggtcctggaa gagatcaacc ggtcgggcgg caacgcgcag
     1621 ccctgccacg cccagatcta caccatgggg gcgctgctgc gccacgccgg cgacgaccac
     1681 aagcgacgct atctcccggc gattgccaaa ggggaactgc gccttcaggc gttcgccgtc
     1741 accgagccgg acgccggcac ggacaccacc agcatctcga cgttcgccga gcgccgggga
     1801 gacaagtacg tggtgcacgg ccggaagatc ttcatctcgc gggtgcagca ttcggagctg
     1861 atgatgctgc tggcccgcac cacccccctc gaccaggtga agaagcgaag ccacgggctt
     1921 agcctgttcc tggtggatct gcggaccgcc gggagccgcc tgcgggtgac ccccatcaag
     1981 accatggtga acaacgagac caacgaactg gtcctcgagg gcgtcgagat ccccgcggag
     2041 aaccggatcg gcgaggaggg cctcgggttc tcctacatcc tgagcgggct gaacgtcgag
     2101 cggatcttga tcgcctcgga gtgcatcggc gacggcacct acttcatcga gcgcgccaca
     2161 cgccgcgcga ccgatcgggt cgtgtttggg cgcccgatcg ggcagaacca gggcgtgcag
     2221 tttcccatcg ccaaggccta tacggcgttg gaggccgcgc gattgatgcg cgacaaagcg
     2281 gctacgcgct tcgaccgggg cgagccgtgc ggcgccgagg ccaacatggc caagtacctc
     2341 gcctcggaag ccgcctggga ggccgcgaac gtggccatga cgacgttcgg cgggtacggg
     2401 atggcggtcg agtacgacat cgaacgcaag ttccgggaga gccgcctcca cctcgtcgcc
     2461 cctgtgtcca acaacctcat cctcagtttt gtcggccagc acgtcctggg gctcccccgg
     2521 tccttctgag cgcgcccatg cgatggccgg acgcggtgac cgtgcacgag gtgggacccc
     2581 gtgacgggtt tcaaatggag aagccgttca tcccgaccga ggtgaagatc cgcctcatcg
     2641 acgccttgtc gcacaccggg ctgcgcgaga tccaggcgac ctcgttcgtc catccccagg
     2701 ccatccctca gttgcgggac gccgaggcgg tgatggccgg catcgagcgc gtgcccgggg
     2761 tggcctatac ggcgctcgtc ctgaacgagc ggggggcgga gcgcgccgtc gcggcgcgcg
     2821 tcgaccggat cgatatcgcc gtctcggcct cggactcgca ctcgttgagc aacgccaaca
     2881 tgacgaccgc ccaggcgatg gaatgcgccg gccgggtggc ggcgatgggc cgggacgcgg
     2941 gcaccgacgt ggccatcggg tttgcgaccg ccctcgggtg ccccttcgag ggggtgctcc
     3001 cgtacgggcg ggtggaggcg ctggtcgccc gcgccgtgga ggagttcggc ctagcacggg
     3061 tggggatcgc ggacaccgtg ggcatggcga acccggccct cgtgtggacc acgatggacg
     3121 cgctgcgccg gcgattcccg gctgcgagct tctcgctgca cctgcacaac acccgcggga
     3181 tgggcctggc gaacatcctg gccgggtgtg atgcgggggt cgcgcatttt gacgcctcgg
     3241 tcgccggtct ggggggatgt ccgtacgccc ccggggcgac gggcaacatc gcgacggagg
     3301 atgccgtcca catgctcgag gagatgggca tcgccacccg cgtgcggctc gacgccctgc
     3361 tggatgtcgc ccggcaggtg gaggaagcgg tggggcacgc ggactcggcg gtcctcaggg
     3421 ccgggaccag ccgcatgctg ctcggcgccc ggcgcggggg gcagacgaag ctcgggacac
     3481 cctgagcccg ggtcaggatc cgagccccgc ccggcgctcc attaggcgga cgagttcggt
     3541 gtgatcccga tcggccatcc cttcttgggc cgcgaccgtc catgcctgtc ggaccagcga
     3601 gcccaacagc atcggcacgc ccagctcatc ggccaagtcg aggcagatgt ccaggtcctt
     3661 gacgtactgg cccacggtga accccgcgtc gaaccggcgg ggcaggaagt accgggagac
     3721 cttggtttcc gtcgtgtagc tgcggcctgt ggagcggttg acggccgcga acagccggtc
     3781 cggcggcagg cccgccttca cccccagggt gagcgcttcc acgacgctcc agatcgtcgt
     3841 cgccgacagg aggttgttga tggtcttggc ggtatcgccg tcgccggcgg catcgccgac
     3901 gtggaccacc tccccgaagc gttccaggat cggccggcag gcctccacga cctcggccgg
     3961 cccgcccgcc atcacgcaca gcgtgccatc ggcggctccg cgcacgcccc ccgacacggg
     4021 gcagtcaagg acgtgcacgc ccttggggcg gaggtcggcg gcgatccggc gggtggttcg
     4081 tggatgggac gacgtcatct cgagcagcac ctgcccggct ctcatggctt ctcccaggcc
     4141 ccgcgcccca tagaccacgc gctcgacggc ccgcgcgtcg ggcagcatcg tgatcaccac
     4201 gtcggcacgc gccgccaccg cggcgggact atcgccggat gcggcgccgg cgttgcacgc
     4261 ctcggccacc gcctcggtcc ggacatcgtg cgcgatgacc cggtaccccg cggccagcag
     4321 cctgtgcgcc atcggcgtac ccatcttgcc gagaccgatg aacccgaccc gctgcagttc
     4381 cgcccggggc acagatgagt ctcccatccc acgctccgat ctccggcatc cgcacgggac
     4441 gccgggctac tgtttgacgg ctccaaaggt gaggccggac acgatgtggc gctggacgac
     4501 aagggtgagg aggatcaccg gcagggtgat catcgtcgcc gcggagaagg ttccagggtg
     4561 accccgctgg ggtgttatgc agaaggcgcc ggaactcctt gtgtccaatc aacgagggca
     4621 cgccgcgggc cgaggcgcgc ccggggggaa agagcgtggc atgagtgaga tccaaacgcg
     4681 cccaggggat ctggaactcc gttctataca tgttcgttac acaaaccgcc gatgatgtgg
     4741 aacgagttct gcgaacaatg cctcacacgg aggaaaaatc acatgagggg cctcatggcg
     4801 tgcgtcatcg cgatcgcctt cctgctcgca tacgccgtgc cgccgaccgg cgcggcgatg
     4861 atgatgggcg cggacgtcaa gaaggagctg caaacggcca tgttccatgc agtcgagctg
     4921 gcccagcggg gcaatgtcgt ggccacttcg caactgcacg tgcagcacgt catcaactgc
     4981 ttggaggggc cgaacggggc gaacttcaag gccgcggcgg gttacccctg tcaggggatg
     5041 gggaacggga tcatccccga cctcaaggac gcggtggccg ccaaggttcc ggg
//
LOCUS       DASQGH010000374         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21328, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000374 DASQGH010000000
VERSION     DASQGH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21328"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="plsX"
                     /locus_tag="VEW91_07300"
     CDS             complement(<1..816)
                     /gene="plsX"
                     /locus_tag="VEW91_07300"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HYM91420.1"
                     /translation="MPGARLRVAVDAMGGDDAPREVVGGAAWAARELGVEILLVGPTH
                     HIQRELETIGAGTLPIRVVEAPEVIDMAEAPAMALRRKRQASILVAVETVRRGDADAV
                     VSAGNTGAAMAASLLTLGRIEGIDRPAIAAVLPTLRGRAVMVDVGANVDCRPKHLLQF
                     AVMGSAYAASVLGIAAPRIGLMSNGAEDTKGNEVVIRAAELLRASSLRFIGNMEGRDF
                     FAGVADVAVCDGFVGNLLLKFGEGLALGIFTMLREELSRGPLVRLGVALATPRL"
     gene            complement(838..1038)
                     /gene="rpmF"
                     /locus_tag="VEW91_07305"
     CDS             complement(838..1038)
                     /gene="rpmF"
                     /locus_tag="VEW91_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016459444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYM91421.1"
                     /translation="MGLTKRRFSKARTASRRAGFKARPVTLVDCPQCHARMVPHRLCP
                     ACGYYAGRQVVEVKAREEATGT"
     gene            complement(1079..1558)
                     /locus_tag="VEW91_07310"
     CDS             complement(1079..1558)
                     /locus_tag="VEW91_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HYM91422.1"
                     /translation="MRVNVGELLADRRAVRVVAFSERIESPGEDTTLLDPVQGELVLT
                     GTGRGVSLTGRVRTALSLVCGACLTQYRQPLEFAVDEEFGRSPVRAGASRGETELGPE
                     DFVVPIGPDGMVDLTEVVRQHLVLAIPIAPRCREGCRGLCAQCGADLNLGPCTCPRA"
     gene            complement(1613..>2183)
                     /locus_tag="VEW91_07315"
     CDS             complement(1613..>2183)
                     /locus_tag="VEW91_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91423.1"
                     /translation="SFRGHSQRGEPMTTPGRSSASPSLATCLDRLEALLAAGPPVFPP
                     SRRAAWEEAREQVRTLRDILATDARRAQALPEAEAVLRRAHDEARRIVLEAEERARRA
                     LEDGTLARAAEQQLQQIREQAVREADETRRGADLYAMTVLERLERETARILATVQRGK
                     TVLSERTGGRTPAGLGSTARLDNGKLASV"
BASE COUNT          295 a          847 c          721 g          320 t
ORIGIN      
        1 cagccggggg gtcgccagcg ccacccccag gcggaccagc gggccacggc tgagctcttc
       61 gcggagcatg gtgaagatgc cgagcgccag cccttcgccg aacttcagca gcaggttgcc
      121 cacaaacccg tcgcagaccg ccacatcggc gacccccgcg aagaaatccc ggccttccat
      181 gttgccgatg aagcgcaggc tcgaggcgcg caggagctcc gcggcgcgga tcaccacctc
      241 gttccccttg gtgtcttctg ccccgttgct catcagccca atgcgcggcg cggcgatccc
      301 gagcacgctg gccgcgtacg cgctccccat cacggcaaac tgcagcaggt gcttgggccg
      361 gcagtcaacg ttggcgccta cgtccaccat gacggcgcga ccgcggaggg tcggcagcac
      421 cgccgcaatc gccgggcgat cgatgccctc gatccgtccc aacgtcaaca gcgacgcggc
      481 catcgccgct ccggtgttgc cggcgctgac gacggcatcg gcgtcgcctc gccgcaccgt
      541 ctcgaccgcc acgaggatcg aggcctggcg cttgcgccgc agggccatcg ccggcgcctc
      601 tgccatgtcg atcacctcgg gcgcctccac gacccggatg ggaagcgtcc ccgcgccgat
      661 cgtctcgagt tcccgttgga tgtggtgggt ggggccgacg aggaggatct ccacgccgag
      721 ttcgcgggcg gcccaggcgg cgcccccgac gacctcccgc ggggcgtcgt ccccccccat
      781 cgcgtccacc gccacccgca gtcgcgctcc cggcatgcgc gtccctcgct ccgctgctta
      841 ggtgccggtc gcttcctcgc gcgctttcac ctcgaccacc tgtcgccccg cgtaatagcc
      901 gcacgcgggg cacaggcggt ggggcaccat ccgcgcgtga cactgcgggc agtcgacgag
      961 ggtgaccggg cgcgccttga agcccgcccg gcggctggcg gtgcgggctt tgctgaaccg
     1021 tcgctttgtc agtcccatgc tgcacttccc ttccccggct ggtgggcccg ggcgaccctc
     1081 atgctctcgg gcaggtgcag ggccccaggt tgagatccgc cccgcactgg gcgcagaggc
     1141 cccggcaccc ttctcggcac cggggggcga tcggtatcgc gaggaccagg tgctggcgca
     1201 cgacctccgt cagatcgacc atcccgtcgg gtccgatggg gaccacgaaa tcctccggcc
     1261 cgagctcggt ctcgccgcgg gacgcgccgg cgcggacggg gctgcggcca aactcctcat
     1321 ccaccgcaaa ttccagcggc tgccggtact gcgtgaggca ggcgccgcac accaggctga
     1381 gggccgtgcg cactcgaccg gtgaggctga cgccgcgccc ggtgccggtc agcaccagct
     1441 cgccctgcac cggatcgagc agcgtggtat cctcacccgg cgattctatc cgttcggaaa
     1501 acgccaccac ccggacggcg cgccggtcag ccaggagctc gccgacattg acacgcacac
     1561 cgccacctca caaggtgtgc ccggcggaca caccgccggg cacaccttgc tatcataccg
     1621 acgccagttt cccattgtca aggcgcgccg tgctccccag gcccgcgggc gttctcccgc
     1681 cggtccgctc gctcagcacc gtcttgccgc gctgcaccgt ggcgaggatg cgcgcggttt
     1741 cccgttccag gcgctcgagc acggtcatcg cgtagagatc ggcgccgcgg cgcgtctcat
     1801 ccgcctcgcg caccgcctgc tcccggattt gctgcagctg ctgctcggcc gcgcgcgcca
     1861 gcgtcccgtc ctccagcgcc cggcgggcgc gctcctccgc ctccaggacg atccgccgcg
     1921 cctcatcgtg cgcccggcgg agcaccgctt cggcctccgg gagggcctgg gcgcgcctgg
     1981 cgtcggtggc caggatgtcc cggagcgtgc gcacctgctc ccgcgcttct tcccacgcgg
     2041 cccgccggct gggcggaaac accggggggc ccgccgccag cagggcttcg aggcgatcca
     2101 ggcaggtcgc cagggacggg gatgcggagg atcgaccggg cgttgtcatc ggttcacctc
     2161 tttgagagtg acctcggaaa ctt
//
LOCUS       DASQGH010000375         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7400, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000375 DASQGH010000000
VERSION     DASQGH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7400"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1736
                     /locus_tag="VEW91_07320"
     CDS             165..1736
                     /locus_tag="VEW91_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91424.1"
                     /translation="MRRGKRVPETLSRRDLLGRVGLGIAAAGLLSATEPGPATAQTTQ
                     KRERAVAQGGDISKFDPHFSTSSNDIRVSFNIFDNLTSRHPDGQLFPGLATEWGRTAP
                     TTWQFKLRQGVKWHNGDPFTSTDAKFSIERTYDPAAKTLVATVFTTIERIEAPDPATL
                     IIHTKRPDPLLAARLAFYGGQIVPNKYLEQVGPDAFNAKPVGTGPVRFVSWTKDDKLV
                     MEATPDYWGGKIDVDRVTFRPIPETAPRVAAVLKGEVDTIAQLPPDQWGRVNQNPTTR
                     GVGRLYAGLYVLAVNSKVKPLDNPRVKQAMSLAIDRVAIVKDLWRGRGIIPNGPIAKG
                     DNHYDPSLPPLPYNPQRARERLREAGYKNEPIYIETTVGYVANDKAMSEAIASMWKDV
                     GLNSVVEVIEYSVRAQKNRERSFKGMFWSDPTSTLGDPDGMMWRLLGPGGPQDNWSHP
                     EFDELGNAARFSLDEKFRDEAYKKMTRILLEHNPWIVVIQPDEDYGLQKYVDFTPNPN
                     QQFEVRRFNFRMRRA"
     gene            1761..2693
                     /locus_tag="VEW91_07325"
     CDS             1761..2693
                     /locus_tag="VEW91_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91425.1"
                     /translation="MRGLGAFLVYRLFRTLIALWLVSTVVFVVMRLSGDPVPLLLPPD
                     APVAEMERVRHDLGLDRPLAVQYAVFIGNVLRGDFGRSIHFRQPAMGVALSYLPATFE
                     LGLAAFVIAVVIAFPVGVLSAVRRNSPLDHGAMALALVGQSAPTFFLGILFILVFSLQ
                     LDLFPTSGRGDWRHLVLPALTLGAFAMASIARITRSAVLEVQRADFIRTARAKGLGEF
                     VVVAKHTLKNAALPILTITGLQFGTLLGGAVVTETVFAWPGMGRLAIQSIYNRDYPVV
                     QSTVFIAAVLFIFINLGLDAVYSVLDPRVRETRA"
     gene            2690..3595
                     /locus_tag="VEW91_07330"
     CDS             2690..3595
                     /locus_tag="VEW91_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91426.1"
                     /translation="MSRPVAAAATLGAATALPAPYPVRRRRVPWPIVAACGFVGALVV
                     VAAAAPYLAPSNPERGSLGARLEAPTLGGADGKAHPFGTDHLGRDVLSRVIYGTRVSL
                     TVGFAAVAIGGAIGGTLGLLVGFYGGWLDEVIMTVADAQLAFPFILLAIGIIAVLGPS
                     FPNLVIVVGLSGWVTYARVLRSQVLSLRRREFVDAIIGLGGSVPRIIVRHILPNVAST
                     FMVIATLELARAIVLEATLSFLGLGIQPPTPSWGGMIQEGRDYLDSAWWISIFPGVVL
                     MLTSLVVSRAGDWLRDVLDPTLRGG"
     gene            3619..4599
                     /locus_tag="VEW91_07335"
     CDS             3619..4599
                     /locus_tag="VEW91_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007997845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91427.1"
                     /translation="MTWLLGMLEGARKVVRTCARIQPGEQVAVVADTDTVDVAEALAA
                     AALEVTPEVVTTVMTPRRVDGAEPPGVVAAVLQAADVALIPVSYSISHSNAVRAALKH
                     GTRVLSLPAATPDQLVRGGAEADFEAQAPTVRRVAERLTNARAARLTAPAGTDCTFDL
                     SGRSGNAHDCILDRRGKFSAFPNIEANISPVDGTAQGVIVFDGSIPNLRLGGPLRHPV
                     VCTVERGSIVKVDGGPEAQMIARVWAALGDAAVYSIAQLAIGLNPEVLVLTGRWAQDH
                     GAFGTVHIGIGTSANLGGTIKAAAHFDGMMYRPTLVLDGEPLLEEGRLRA"
BASE COUNT          669 a         1627 c         1602 g          754 t
ORIGIN      
        1 ccggactggg ggccgcgcgc gtctttggga gtgctcccga ctccccatag gggagcggga
       61 ccccgtgatg tgtgcaatgg ccgcaatgat gcaggaagat cgttgacgca ggagaagcct
      121 ctaggttgta cgtcctttgt atccgcacct ggaggaggtc gatcatgcga cgaggcaagc
      181 gagttcctga gacattgtcg cgccgcgatc ttctcggccg ggtcgggctg ggcatcgcgg
      241 cagccgggct cctgagcgcg accgagcccg ggcccgccac cgctcagacc acacagaagc
      301 gggagcgggc cgtcgcccaa ggcggggaca tctccaagtt tgatccccac ttctctacct
      361 cgtcgaacga catccgggtc tccttcaaca tcttcgacaa cctgaccagc cgccacccgg
      421 atgggcagct cttccccggg ctcgccaccg agtgggggag gaccgcgccc accacctggc
      481 agttcaagct ccgccaggga gtgaagtggc acaacggcga ccccttcacg tcgaccgacg
      541 cgaagttctc gatcgagcgg acctacgacc cggcggcgaa gacgctggtc gcgaccgtgt
      601 tcacgaccat cgagcgcatc gaggcccccg acccggccac cctcatcatc cacaccaagc
      661 ggccggatcc gctcctggcg gcgcggctcg ccttctacgg cggccagatc gtccccaaca
      721 agtacctcga gcaggtgggc cccgacgcgt tcaacgcgaa gccggtgggg accggccccg
      781 tccgcttcgt ctcgtggacc aaggacgaca agcttgtgat ggaggcgacc cccgactact
      841 ggggcgggaa gatcgatgtg gaccgggtga ccttccggcc gatccccgag acggcgccgc
      901 gcgtggccgc cgtcctcaag ggtgaggtgg acacgatcgc ccagctcccg cccgaccagt
      961 ggggccgtgt gaaccagaac ccgacaaccc gcggcgtcgg caggctctat gcggggctct
     1021 atgtgctggc cgtcaactcg aaggtcaagc cgctcgacaa cccgcgcgtg aagcaggcga
     1081 tgtcgctcgc gatcgaccgc gtggcgatcg tcaaggacct gtggcgggga cgcgggatca
     1141 tccccaacgg cccgatcgcg aaaggcgaca atcactacga cccctcgctc cccccgctcc
     1201 cgtacaatcc gcagagggcc cgcgagcgct tgagggaggc cggctacaag aacgagccca
     1261 tctacatcga gaccaccgtc gggtacgtgg caaacgacaa ggcgatgtcc gaggccatcg
     1321 cctccatgtg gaaggacgtc gggctcaact cggtggtcga agtgatcgag tactccgtcc
     1381 gcgcccagaa gaaccgggag aggtccttca aggggatgtt ctggtccgat ccgacctcga
     1441 ccctcggcga tcccgacggg atgatgtggc ggctcctcgg tcccggcggg ccccaggaca
     1501 actggagcca tcccgagttc gatgagctgg ggaacgcggc ccgcttcagc ctggacgaga
     1561 agttccgcga cgaggcatac aagaagatga cccggatctt gctcgagcac aacccctgga
     1621 tcgtcgtgat ccagccggac gaggactacg gccttcagaa gtacgtcgat ttcacgccga
     1681 acccgaacca gcagttcgag gtccgccgct tcaatttccg catgcgccgc gcgtaacgcc
     1741 gccgccgccg ggcgttcgtg atgcgcggcc tcggcgcctt tctcgtctac cggctgtttc
     1801 gcacgctcat cgcgctctgg ttggtctcga cggtcgtctt tgtggtgatg cggctgtcgg
     1861 gtgacccggt gccgctcctg ctcccgcccg acgccccggt cgccgagatg gagcgcgtcc
     1921 gccacgacct cgggctcgac cgcccactcg cggtccagta cgccgtgttc atcgggaacg
     1981 tcctccgcgg agactttggg cgctcgatcc acttccgcca gccggcgatg ggggtggcgc
     2041 tttcgtacct gccggccacg ttcgagctgg gcctggccgc gttcgtgatc gcggtcgtga
     2101 tcgcgttccc ggtgggcgtc ctctccgcgg tccgccggaa ctcgccactc gaccacggag
     2161 ccatggccct ggcgctcgtc ggccagtcgg ccccgacgtt ctttctcggc atcctcttca
     2221 tcctggtgtt ctccctccag ctcgacctct tcccgacctc gggccggggg gactggcggc
     2281 acctggtcct gccggccctg accctcggcg ccttcgccat ggcctcgatc gcccgcatca
     2341 cgcgctcggc ggtgctggag gtccagcgcg ccgatttcat ccggacggcg cgggccaagg
     2401 ggttgggaga attcgtggtg gtcgcgaagc acacgctcaa gaacgcggcg ctgcccatcc
     2461 tcacgatcac gggcctccag ttcgggacgc tgctcggcgg cgccgtcgtg accgagacgg
     2521 tcttcgcgtg gccgggaatg gggcgcctgg ccatccagtc gatctacaac cgggactacc
     2581 cggtcgtgca gtccacggtc ttcatcgccg cggtgctgtt catcttcatc aacctcgggc
     2641 tcgacgccgt gtacagcgtg ctcgatccgc gggtccggga gacgcgcgcg tgagccggcc
     2701 ggtcgccgct gcggccacgc tcggggcggc gacggcgctg cccgcgccgt atccagtccg
     2761 ccgccggcgc gttccctggc ccattgtcgc cgcgtgcggc ttcgtcgggg cgctggtggt
     2821 ggtggcggcg gccgcgccgt acctggcgcc ctccaacccc gagcgcggct cgctgggggc
     2881 ccggctcgag gcgccgacgc tcgggggggc cgacggcaag gcgcacccgt ttgggacgga
     2941 tcacctgggg cgcgatgtcc tctcgcgggt gatctacggc acccgcgtgt cgctgaccgt
     3001 gggctttgcc gcggtggcca tcgggggagc gatcggcggc acgctcgggc tcctcgtggg
     3061 cttctacggc ggctggctcg acgaggtgat catgacggtg gccgatgccc agctcgcgtt
     3121 cccgttcatc ctgctcgcca tcgggatcat cgcggtcctc gggccgtcgt ttccgaacct
     3181 cgtcatcgta gtgggcctgt ccggatgggt gacctatgcc cgggtgctgc gatcccaggt
     3241 gctctcgctc cgccggcgcg agttcgtgga cgcgatcatc ggactcgggg ggtccgtccc
     3301 gcgcatcatc gtccggcaca tcctccccaa cgtggcctcg accttcatgg tgatcgcgac
     3361 cctcgagctg gcccgggcca tcgtgctcga ggccacgctc tccttcctgg ggctcgggat
     3421 ccagccgccg accccgtcgt ggggcggcat gatccaggag gggcgcgact acctggacag
     3481 cgcctggtgg atctcgatct tcccgggcgt ggtgctgatg ctcacctcgc tcgtggtgag
     3541 ccgggcgggc gactggctcc gggacgtgct ggacccgacg ctccgcgggg ggtagcgcgt
     3601 ggcgcgcgga ggcccacgat gacttggctg ctggggatgc tcgagggggc gcgcaaggtg
     3661 gtccgcacgt gcgcgcgcat ccagccgggt gaacaggtcg ccgtcgtggc ggacaccgac
     3721 accgtggatg tcgccgaggc gctcgcggcc gcggcgctgg aagtgacgcc cgaggtggtg
     3781 acgaccgtga tgaccccgcg gcgggtggac ggcgccgagc ccccgggcgt tgtggcggcg
     3841 gtgctgcagg ccgcggacgt cgcgctgatc ccggtcagct actcgatcag ccactccaat
     3901 gcggtgcgcg cggcgctcaa gcacgggacc cgcgtgctct cgctgccggc ggcgaccccg
     3961 gatcagttgg tccggggcgg ggccgaagcg gacttcgagg cgcaggcccc gaccgtgcgg
     4021 cgcgtggcgg agcgcctgac caacgcccgg gccgcccgcc tgaccgcgcc cgccggcacg
     4081 gactgcacct ttgatctctc cggccggtcc ggcaacgcgc acgactgcat cctggaccgg
     4141 cgggggaagt tttccgcctt ccccaacatc gaagccaaca tctcgcccgt cgacggcacg
     4201 gcgcagggcg tgatcgtctt cgacggaagc atccccaatc tgcggctggg cggcccgctt
     4261 cgccatcccg tggtctgcac ggtcgagcgc gggagcatcg tgaaggtcga cggcgggccc
     4321 gaggcgcaga tgatcgcgcg ggtctgggcc gcgctgggcg acgccgcggt ctacagcatc
     4381 gcgcagctcg caatcgggct gaaccccgag gtcttggtgt tgaccggccg atgggcgcag
     4441 gaccacggcg cgttcggcac ggtccacatc gggatcggca cgagcgcaaa cctgggcggt
     4501 accatcaagg ccgccgcgca cttcgacggg atgatgtaca ggcccacgct ggtgcttgac
     4561 ggggagcccc tcctggagga gggccgcctg agggcgtgac ctgatctggt tacgcggcga
     4621 tgcagtatct gacctcgcga aacggcgcgc gc
//
LOCUS       DASQGH010000376         8255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49759, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000376 DASQGH010000000
VERSION     DASQGH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49759"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VEW91_07340"
     CDS             <1..809
                     /locus_tag="VEW91_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYM91428.1"
                     /translation="AAHAYKAVLVVHNETSTGVTSDVRAIREAIRAARHPALLLVDVV
                     SALGSLDFRFDEWEVDVALTGTQKGLMLPPGMAILCASPRAIEASAGATAPRYFFDWR
                     PVLEETRRGYFPYTPATLMLYGLREAVRMLLEEGLPNVFARHRRLAEAVRRAVAASGL
                     SILCRDPALVSHSLTAVVVPAGADAEAVVRGAAQRLNLALGTGLGRLKGKVFRIGHLG
                     SLNELEVIATVAGVEMACTAAGIRVPLGAGVTACQQFLMEAAMAATRTAG"
     gene            complement(909..3047)
                     /locus_tag="VEW91_07345"
     CDS             complement(909..3047)
                     /locus_tag="VEW91_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYM91429.1"
                     /translation="MIRVAAPVLLALVLGLGWPSPAAQPATDNDLGKAVDAYGRGNLT
                     QARALLDNLQQDPGPLAGRAAYLLGIINLQQRKFTLAEAVFSLAADKLPILADHAHYY
                     RAVAAFHAGDYALATVGFEEVLSRYPSSTLRGLALFWEAESLWGVHSPDAPAAFHRYL
                     EEYGQGDHAAQAWFDMGEALMQLGRWEEAAQAYRRVRWGFEGSPFWQPAWAHLTALGA
                     AHRLPLDTTPPEVFYRRAMADFDAGESRLAREEFLRALAMPGGWRIAPDALYQIGVLS
                     FQRGRLDEAASYFQRVAGLHGAHGDESLYWLERIALKRNRDAEALTIARELIRAYPQS
                     ELAPRALYAIAEVREDKGALGPAQGLYREAGERFPETQWGQRALWAVGWVQYRARQWG
                     AARDEWLRISQGSNGEVAPAAHYWAARAAESLGRTDLAQEEYRRVASQYPDSYYGHRA
                     ATRLSVPVRATVVPLSEIPPGAVLAFDRFRELDMLAQIDDATSELAAAAEKAPGKYQA
                     AVGALLSQRYAQQSEIGHGIATAEQVRDLVGGAGHGLPLILWEALYPQAFWEAITRAA
                     ERVKVDPYLVAAMIREESRFDPQATSGASAYGLMQLVPGTARGAARLARIPAPGIQGL
                     RDPETNILLGSVVLHELLGRFTRVDLALAAYNAGPDVVGRWQAKRGGMDPETFVEEIP
                     YVETRNYVKTVMQSAAMYRWLYRDGHPSSP"
     gene            complement(3489..5633)
                     /locus_tag="VEW91_07350"
     CDS             complement(3489..5633)
                     /locus_tag="VEW91_07350"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HYM91430.1"
                     /translation="MLASTGFTVPVASGIFYSHFALTTESGPLDIHHLRIDLANPTVR
                     LGIGLARDRLISDDETVSSMVLRSGAIAGVNGDYFDIRDSGAPINILVRDGVLLRSPW
                     RFVALAIAKDGMARVVRFRWTGTLAIPETGETHPLDGYNSGIAQDGIIAISDIRGYGA
                     PPPDPGTRQTVVELSPANDSTLMLVKPDPATPPAPAGESNRYFVKQVWTQQAFYAPFP
                     KTELILLGRGPGADWLARRLTAGQQVQVNLTTDPDWRDLQGAIGGGPVLVQDGHIVDD
                     PDAPAPREQDRRHPVIAIGIARDGRRLTLVEVDGRQPNFSIGLTRRELASYMHWLGVY
                     QAMAFDSGGSATVVARLPGQPVPTVVNSPSDGRERPVANALLVYTTSVLGPPLTLLVN
                     ANQPLRLYAGAAFPLSIIGLDAQGNPVPPTEPLRVSASPPLVAYGGGSVRAGAGGGQG
                     VLHVQSGPAAGTTRVSVVTQLRRLVVSPEALSLVPGAGWLFTMAGQDAEGHAVALPDT
                     AGAWAVNPPWLGTFSAPGAFVAGERQGAGTIRAHLGGASAQVRVVIVNTARPLNQFDR
                     GDWAFRGYPDTVTGSVALVTAPSHEHRPSAQLMFNLDGPSNRAAYLVTRLAISGAPTG
                     VTLWVYGDGSGVWLRGTYEPSSGPPGSVTFARRVTWQGWRSVTAQLPPGLAYPITWTS
                     FHVVESDPNRSPHGVLYLSSLRAIYAQPTARR"
     gene            5826..7451
                     /locus_tag="VEW91_07355"
     CDS             5826..7451
                     /locus_tag="VEW91_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM91431.1"
                     /translation="MATNRPEVRVSERDRPLIWTLLLMAVCLLVAGLPLPLTDGVTAL
                     YGKIAKNILASGDWLTLRHSQMPVVDKPPLTFWLMSLTFAAFGTAEWVLRLWHLALAV
                     ATALATYALGRLALPRSEALLAGLILLTTTQFFYQSLVPEQHVPLALFLTLAVYWQLR
                     WERAGRWVAAVLAWLAAALAALTIGIAGVVMIALVIGAHLIVDRPRLPRGALRVAMLG
                     AAVFLAVAAPWFVIGVLRQGRPFAATFFLGGTLGVGRFFHPVQASPTVVPWWAGFGAY
                     LLLLPLGFLPWTGWLWAALRDGWAARRTDASPLWVCTVWVIVVVGFLSLSLGDKASRY
                     LLPVFPPLAVLVAHAVGKVNWARQAAAIALATALPLLAVVTTVALVKFPADSARYAPL
                     FWSFLPAFTAALVAYAAATFLGRPKAGIVLLVLMTLLAYGLTMASAARIWDEISPWRP
                     IARTVNGLGSVRARLLILGAYNELADYYITGPVEFVGPGDLVRAWAHERVVAVIPQAL
                     VPALPSPAPVSVSTAPAGLAVVSNFPAPGLPPR"
     gene            complement(7448..>8255)
                     /locus_tag="VEW91_07360"
     CDS             complement(7448..>8255)
                     /locus_tag="VEW91_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYM91432.1"
                     /translation="AGTLVVNRVASDRWKIEGPHSWPADDFKVTDLFFPLTTSEAKAF
                     HDGVVDLSRYDLDHPTVTVDLTLRDRQEPLRILLAQRGKVTYAAVAGSQTVLELDASV
                     QGKLTPDPNALISRRVLPYNAQNLTGVSWRRGGQTFELRRQGPGFTGGGLSESEVTGM
                     FSVVNLLDADRVEPLGPLPSGAPAFELQTDGAADAQFLVQFYREPAGGWLAIDGPLAL
                     QYHLTANALDGLPGKLKAFLGLAQPAAQPAKPGPGAAPTTPSQPPPQPKP"
BASE COUNT         1181 a         2941 c         2837 g         1296 t
ORIGIN      
        1 acgccgccca cgcctacaaa gcggtcctgg tggtgcacaa cgagacctcg accggggtca
       61 cgtcggatgt tcgggcgatc cgggaggcga tacgggcggc caggcatccg gcgctgctgc
      121 tcgttgacgt ggtgagcgcg ctcggcagcc tcgacttccg cttcgacgaa tgggaggtgg
      181 acgtggcgct gaccggcacg caaaaggggc tgatgctgcc cccgggcatg gcgatcctct
      241 gcgccagccc gcgtgcgatc gaggcgagcg ccggagccac cgctccgcgg tacttcttcg
      301 actggcggcc ggtcctcgag gaaacccggc gcggctactt cccgtacacc cccgccacgc
      361 tcatgctcta cggcctgcgc gaggccgtca ggatgctcct cgaggaaggg ctcccgaatg
      421 tcttcgcccg ccacaggcgg ctggccgagg cggtccggcg ggccgtggcg gcgagcggcc
      481 tctccatcct ctgcagggac ccggcgctgg tctcgcacag cctgaccgcg gtggtcgtgc
      541 cggccggggc ggacgccgag gccgtcgtcc gcggagcggc ccagcgtcta aacctcgcgc
      601 tcggcacagg gctcgggcgt ctcaagggca aggtcttcag gatcggccac ctcggatcgc
      661 tcaacgaact ggaggtgatc gccacggtcg ccggcgtgga gatggcgtgc accgccgccg
      721 ggatccgcgt cccgctgggg gccggcgtga ccgcctgcca gcagttcctg atggaggccg
      781 caatggccgc aacccgcacg gccggctgat cgcgaccgac cgcccctaca gatcgtcgat
      841 cggcacaggc tcgaccggat ccgcgagggg gctatccgtc cagcgcccgc gtgatggtca
      901 cggcccggct agggcgacga cgggtgccca tcgcggtaga gccacctgta catcgcggcg
      961 gactgcatca ccgtcttgac gtagttgcgg gtctccacgt acggaatctc ctccacgaac
     1021 gtctcaggat ccatgccgcc ccgcttggcc tgccaccggc cgaccacgtc cggtccggcg
     1081 ttgtaggcgg cgagggccag gtccacccgg gtgaaccgac cgaggagttc gtggagcacg
     1141 acgcttccga gcaggatgtt cgtctctgga tcccggagcc cctggatgcc gggggccggg
     1201 attctcgcca aacgcgcggc gcccctggcg gttccgggca ccagctgcat caatccgtac
     1261 gcgctggcac cggaggtcgc ctggggatcg aaccggcttt cctcgcggat catcgccgcc
     1321 acgaggtacg ggtcgacctt cacgcgttcg gccgcccggg tgatggcctc ccagaatgcc
     1381 tgggggtaga gcgcctccca gaggatgagc gggaggccgt gcccggcgcc cccgaccaga
     1441 tcccggactt gctccgccgt cgcgatcccg tgaccgatct cgctctgctg cgcgtatcgc
     1501 tggctgagaa gcgcgcccac cgcagcctgg tacttgccgg gggctttctc ggcggccgcc
     1561 gccagctcgc ttgtcgcatc gtcgatctgc gcgagcatgt cgagttcccg aaaccggtcg
     1621 aacgcgagga ccgccccggg ggggatctcg gagaggggga ccaccgtggc gcgcacgggc
     1681 acgctcaggc gggtcgcggc gcggtggccg tagtaggagt cggggtactg cgaagccacc
     1741 cggcggtatt cctcctgggc caggtcggtc cgcccgaggg attctgccgc gcgggccgcc
     1801 cagtagtggg ccgccggggc cacctctcca ttcgatccct gggagatccg aagccattca
     1861 tcccgggccg ctccccactg ccgggcccgg tactgcaccc accctaccgc ccacagcgcg
     1921 cgctgccccc actgcgtctc gggaaagcgc tcgccggcct ctcgatagag cccctgggcg
     1981 ggtcccaagg cgcccttatc ctcccgcacc tcggcgatcg cgtacagggc gcggggcgcc
     2041 agctcggact ggggatacgc gcggatcaac tcgcgggcga tggtgagggc ttccgcgtct
     2101 cggtttcgct tgagggcgat ccgctcgagc cagtacaggg actcgtcccc atgcgctccg
     2161 tgcagacccg cgacccgctg gaagtagctc gccgcctcgt cgaggcggcc ccgctggaaa
     2221 ctcagcacgc cgatctggta gagcgcgtcg ggggcgatcc gccacccgcc gggcatcgcc
     2281 agcgcgcgca gaaattcttc ccgggcgagc cgggactccc ccgcgtcgaa gtcggccatg
     2341 gcccgccggt agaatacctc cggcggggtc gtgtcgagcg gcaggcgatg cgccgccccc
     2401 aacgcggtga ggtgcgccca cgccggctgc caaaaggggc tgccctcgaa tccccaccgg
     2461 acccgccggt acgcctgcgc cgcctcttcc caccggccga gttgcatcag ggcttcaccc
     2521 atgtcgaacc atgcctgcgc ggcgtggtcc ccctgaccgt actcttccag gtagcggtgg
     2581 aacgccgccg gagcgtcggg ggagtgcacg ccccacaggc tttcggcctc ccaaaacagc
     2641 gccaggccgc gcagcgtgct gctcgggtat cgggacagaa cctcctcaaa ccccacggtg
     2701 gccagcgcat agtccccggc atggaacgcg gcgaccgccc gatagtagtg cgcgtgatcg
     2761 gcgaggatcg gcagcttatc cgccgccagg ctgaacaccg cctcggccag cgtgaacttt
     2821 cgctgctgga ggttgatgat gccgagcaga tacgccgcac ggccggccag cgggccggga
     2881 tcttgttgga ggttgtccag cagcgctctg gcctgggtga ggttcccgcg gccgtaggca
     2941 tccaccgcct tcccgagatc gttatcggtc gcgggttggg cggccgggga gggccacccg
     3001 aggcccagca cgagcgcgag cagcaccggg gcggcgaccc ggatcatccg actccccaga
     3061 ggacgggctc cgcgccaaag gcgcgacagg ccgcgcggac gatcacgcca gcaccgatca
     3121 ccggatccag aaacgtctac ttctcttcca cgcactcaga attgactggc acgctgtcgt
     3181 gatgagccca cgcccaataa ttgcattggg cttctgtctc gaacggtcca gcgatgtggc
     3241 ttccaccagg cgctgacacg agccaccaag caaacgacac cccaaccatc gctattgcca
     3301 gaatccgtct catctcccgc ctccctcctt cggctcatca agaacctggc tatgaagcgt
     3361 aaccctcaca ccttcaataa ttgccatgat cgcgatctcc atagacacga taggcaaggc
     3421 ggtgcgtgta gcggaagggg gggagagttc gacgatccgc gcaacgcccc ctgcgggccc
     3481 cggcaggcct accttcttgc tgtgggctgc gcgtagatgg cgcggaggct gctgagatag
     3541 aggacgccgt ggggtgagcg gttgggatcg ctctccacga cgtggaacga cgtccacgtg
     3601 atcggatagg ccagccccgg cggcaattgg gcggtcaccg agcgccagcc ttgccaggtc
     3661 acccggcggg cgaaggtcac gctgccggga ggtccgctcg acggctcata cgtgccccgg
     3721 agccacaccc cgctcccgtc cccgtacacc cacagggtca ccccggtcgg ggcacccgag
     3781 atcgccagtc gggtgaccag gtacgccgcc cggttggatg gcccgtcgag attgaacatc
     3841 agttgggccg acgggcgatg ctcatggctc ggggcggtga cgagggcgac cgaaccggtc
     3901 acggtgtccg ggtagccgcg gaacgcccag tcgccgcgat cgaattgatt gaggggtcgg
     3961 gccgtgttga cgatcacaac gcggacctgt gcggacgctc ccccgagatg ggcccggatc
     4021 gttccggccc cctggcgctc ccccgccacg aacgctcccg gcgcggaaaa cgtgccgagc
     4081 cacggcgggt tcaccgccca cgcccccgcg gtgtccggga gcgcgacagc gtgaccctcg
     4141 gcgtcctggc cggccatagt aaagagccac cccgcgcccg ggacgaggct cagcgcctcc
     4201 gggctcacca ccagtcgccg gagctgggtg acgaccgaga cccgggtcgt gcccgcagcc
     4261 ggtccgctct gcacgtggag cacgccctgc cctccccccg cgcccgcgcg gaccgacccg
     4321 ccgccgtacg ccaccagagg gggcgaggcg gacacccgga ggggctcggt gggcggcacg
     4381 ggattgccct gcgcgtcgag gccgatgatc gagagcggaa acgccgcgcc ggcgtacagc
     4441 cggagcggct ggttcgcgtt gaccaggagc gtgagcgggg gccccagcac cgaggtggtg
     4501 tacaccagca gcgcgttggc gaccggccgc tcccggccgt cggacggcga gttgaccacg
     4561 gtcggaaccg gttggccggg gagccgggcg acgacggtgg cggatccccc gctgtcgaac
     4621 gccatggcct ggtagacccc gagccagtgc atgtacgacg ccagttcccg ccgggtgagc
     4681 ccgatgctga agttcggctg cctgccgtca acctccacca gcgtgagccg gcggccatcg
     4741 cgcgcgatgc cgatcgcgat caccggatgc cgccggtcct gctcccgggg ggcgggggca
     4801 tcgggatcgt cgacgatgtg gccgtcttgc acgaggaccg gcccgccccc gatcgccccc
     4861 tggaggtcgc gccagtcggg atcggtcgtg aggttaacct gcacctgctg gccggcggtc
     4921 agccggcggg ccagccagtc cgcaccgggt ccgcggccca gcaggatgag ctcggtcttt
     4981 gggaacggcg cgtagaacgc ctgctgcgtc cagacctgtt tgacgaaata gcggttgctc
     5041 tcgcccgccg gcgcaggggg cgtcgccgga tccggcttga ccagcatcag ggtgctatca
     5101 ttcgcggggc tcagttcaac gacggtctgg cgcgtcccgg gatcgggggg cggggccccg
     5161 tacccccgga tgtccgagat ggcgatgatc ccgtcctggg cgatgccgct gttgtagccg
     5221 tcaagcgggt gcgtctcccc ggtctcgggg atcgcgagcg tcccggtcca gcgaaaccgg
     5281 acgacccgcg ccatcccatc cttggcgatg gccagcgcca cgaaccgcca cgggctgcgt
     5341 agcagcacgc catcccgcac gaggatgttg atgggcgccc cggaatcgcg gatatcgaag
     5401 tagtccccgt tgacgccggc gatggctccg ctgcgcagga ccatggagga caccgtctcg
     5461 tcatcgctga tcagacggtc gcgggccagg ccgatgccga gccgcaccgt ggggttggcc
     5521 aggtcgatcc gcaggtggtg gatgtcgagc ggcccgcttt cggtggtgag ggcgaaatgg
     5581 ctgtagaaga tccccgaggc gaccggtacg gtaaatcccg tcgaggccag cacggggggc
     5641 cacgcagggg ggacctgcgc cagaccttgc cctgctgcgg cgaggagggc agcggcgact
     5701 agccaaccga gtatcctgcg atgtctgcgt cctggcatcc tgcaaccgtc cggtgtcgcg
     5761 atctccgcgt cgatccctga gaattcgcgg gggtggcgcg cgatcctgca caggagaccc
     5821 ctcccgtggc gacgaaccgc cccgaagtgc gcgtctccga gcgcgaccgt cccctcatct
     5881 ggaccctcct gctcatggcg gtctgcctcc tcgtcgccgg gctgccgctg ccgctcaccg
     5941 atggggtcac cgccctttac ggaaagatcg ccaagaatat cctggcctcc ggagactggc
     6001 tcacgctccg tcactcgcag atgccggtcg tggacaagcc gcccctgacg ttttggctca
     6061 tgagcctcac gttcgccgcc ttcggcacag cggagtgggt gctccggctc tggcatctcg
     6121 cgctcgcggt ggcgacggcg ctcgccacct atgcgctggg ccgcctggcc ctcccccgga
     6181 gcgaagcgct gctcgccggc ctcatcctcc tgaccacgac gcagttcttc taccagagcc
     6241 tcgtcccgga gcagcacgtg ccgctcgcac tctttctgac gctggcggtc tactggcaac
     6301 tccgctggga gcgcgcggga cggtgggtcg ccgcggtcct cgcgtggctc gccgcggccc
     6361 tggcggccct cacgatcggg atcgcggggg tcgtcatgat cgccctcgtc atcggcgcgc
     6421 acctgatcgt cgaccgaccc cggctccccc gcggtgccct tcgcgtcgcg atgctcgggg
     6481 cagccgtctt cctggcggtg gccgcgccct ggttcgtgat cggcgttctc cgccagggac
     6541 ggccgttcgc cgccaccttc ttcctcgggg ggacccttgg cgtcggacga ttctttcatc
     6601 cggtgcaggc ctcccccacc gtggtgccct ggtgggccgg attcggggcg tacctcctgc
     6661 tgctcccgtt gggattccta ccctggaccg gctggctctg ggcggcgctg agggacgggt
     6721 gggccgccag gcgcacggat gcctccccgc tgtgggtgtg cacggtgtgg gtgatcgtgg
     6781 tggtgggttt cctgtcgctc tcgctcgggg acaaggcgag ccggtacctg ctccccgtct
     6841 tccctccgct cgcggtgctc gtcgcccacg cggtgggcaa ggtcaactgg gccagacagg
     6901 cggcggcgat agcactcgcc accgcgctgc ctctgctggc ggtggtgacc accgtggcgc
     6961 tcgtgaagtt tcccgcggac tccgcccgct acgcgccgct gttctggtca ttccttcccg
     7021 cctttaccgc tgcgctcgtg gcgtacgccg ccgcgacctt tctcgggcgc ccgaaggcgg
     7081 gaatcgtgct gctcgtgctc atgaccttgc tggcctacgg cctgacgatg gcctcggcgg
     7141 cccgcatctg ggatgagatt tccccgtggc gcccgatcgc cagaaccgtc aatgggctgg
     7201 gaagtgtgcg cgctcgcctg ctcatcctgg gagcctacaa cgaactggcc gactactaca
     7261 tcaccgggcc ggtggagttc gtgggccccg gggacctcgt ccgtgcatgg gcgcacgagc
     7321 gggtggtcgc ggtcatcccc caggccctgg tgcctgcgct gccatccccc gctccggtat
     7381 cggtcagcac cgcgccggcg ggcttggcgg tcgtgagcaa cttcccggca cccgggcttc
     7441 cgccccgcta aggtttgggc tgaggagggg gctgggaggg cgtcgtgggc gctgcccccg
     7501 gtcccggctt ggcgggctgt gcggcgggct gcgccagtcc gagaaacgcc ttgagctttc
     7561 ccgggaggcc gtcgagggcg ttggccgtca gatggtactg cagcgccagc ggtccgtcta
     7621 tcgccagcca tccccccgca ggttcccggt agaactgcac gaggaactgc gcatcggcgg
     7681 ccccgtcggt ttgcagttcg aacgcgggcg cccccgaggg caagggtccc agcggctcga
     7741 cccggtcggc atccaggagg ttcaccaccg agaacatgcc ggtgacctcc gactccgaca
     7801 gcccaccgcc ggtgaagccc ggcccctggc gcctgagctc gaacgtctgg ccaccccggc
     7861 gccagctcac gcccgtcagg ttttgcgcgt tgtatgggag cacacgccgg ctgatgagtg
     7921 cgttgggatc gggggtcagc ttcccctgga ccgaggcatc aagttccagg acggtctggg
     7981 accccgcgac cgccgcgtag gtgaccttcc cgcgctgggc gagcaggatg cgcagcggct
     8041 cctggcggtc ccgcagggtc aggtccacgg tcaccgtggg gtgatccagg tcataccggg
     8101 agagatccac cacgccgtcg tgaaacgcct tggcctccga tgtcgtcagc gggaaaaaca
     8161 ggtccgtcac cttgaaatcg tccgccggcc acgaatgcgg cccctcgatc ttccaccggt
     8221 cgctcgcgac tcgattcacg accagcgtcc cggcg
//
LOCUS       DASQGH010000377         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49762, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000377 DASQGH010000000
VERSION     DASQGH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49762"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..519)
                     /locus_tag="VEW91_07365"
     CDS             complement(31..519)
                     /locus_tag="VEW91_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HYM91433.1"
                     /translation="MSVDGGGLHAYVAAAKVKTRAGLGVVFVDSQGRVLRRIGRCVPA
                     PHEDLAGFQGILYALWTARRLGSRRVVVHSDNAAVVAQINGHQEVRQDLVGPYLEVRA
                     LLHAYRSARVEASQLGSTQGAPAMAEAALTFDMNDLDVTEVVVEDLPLWSWQGAAERA
                     PA"
     gene            1050..1505
                     /locus_tag="VEW91_07370"
     CDS             1050..1505
                     /locus_tag="VEW91_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91434.1"
                     /translation="MNAALAANDRLKYSFSILQMALAHADHPEQPASTLRRERLAAGI
                     DDPRLDDTVATAQRDGDRYRAPGSAKVPAGLKIPVSAVQLRPPAPALFHRICERSVEG
                     QRCGEFCADYRRSDRAFAEAPIRLRERLKVPRSAGVPIEPRGILALTPP"
     gene            complement(1529..>1977)
                     /locus_tag="VEW91_07375"
     CDS             complement(1529..>1977)
                     /locus_tag="VEW91_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91435.1"
                     /translation="LAGAGAGVVAALASVIAEIIMRAQLPPRVATLWSAFVAGILGGI
                     LYGWLGRAVRRPIVALWVITLAIATIDSLLIATLPFALGPNPSIGIPIVGLIVPVRQL
                     LALAGVGHLGTRHFPSQYLLADTMTHYITAVAVSLLVPWWAKPKNL"
BASE COUNT          363 a          648 c          648 g          318 t
ORIGIN      
        1 taggagactc gatgaactct tcccggcagg tcaggcggga gcccgctcgg ccgccccctg
       61 ccaggaccac agcggcaggt cttcgacgac cacctcggtg acatcgagat cgttcatgtc
      121 gaaggttagg gcagcttcgg ccatcgccgg ggcgccctgg gtcgatccca actggctggc
      181 ctcgacgcgg gcgctccggt aggcatggag gagcgctcga acctccaagt agggtcccac
      241 gaggtcctgt cgcacctcct ggtggccgtt gatctgagcg acgacggcgg cgttgtcgct
      301 gtggacgacg acccggcggg atccgagccg ccgggctgtc cagagcgcgt acaggatacc
      361 ctggaacccg gcgaggtcct cgtgtggggc gggcacgcag cgaccgatcc ggcggagcac
      421 ccggccctgc gaatcgacga acaccacccc caggccggcc cgcgtcttga ccttggcggc
      481 ggcgacgtag gcgtgcagcc cgccgccatc cacgctcatc gtgccaactc gagcacggcc
      541 gtcaacacgc cgatcccgag gagaagggcg accttcaggc cgagaagatc gcgatcgtcc
      601 ccgaagcgtc gcgagagcgt tcgaactgtc gctcgccgcg gggaaggggg gaggggtgcg
      661 tgcgacccag cccgctgtct ccatccttcg gaggggacgc cagctcgctt ggatctggct
      721 ccttgcggcg cgcgtgtggc tggggagcca gaccttatga cgtcctcccg gtcgagacgg
      781 agtgccgagt gcacccaccc ctcctttgca gggaccgccc ggtgaggctt gtttgactcc
      841 catgatagaa cggcggagtc aagcgcaggt gaagcccggc aaaaccctgg gtgaagagcg
      901 cgtgaaatcg cctgagacgg cttcatccag ccttcgactg tccttaaccg ggggttaacc
      961 ttcgcttcct agcccgtgat cctagcctgt gagtggaggg gtgggcccgc tcgcccccga
     1021 ttcctggatc gtgaaggcgc ccaggccagg tgaacgcggc actggcggcc aatgaccgtc
     1081 tcaaatactc cttttccatc ctgcagatgg cactggcgca cgccgaccac ccggagcagc
     1141 cggcgagcac gctgaggcgt gagcgcttgg ccgctggtat cgatgatccc cggctggacg
     1201 acactgtggc caccgcacag cgcgatggag atcgctatcg cgcgcccggc tccgccaagg
     1261 tgcctgcggg actgaaaatc cccgtgtcgg cggttcaact ccgtcccccg gcaccagcct
     1321 tgtttcatag gatttgcgag cgaagcgtgg agggacaacg ttgcggtgag ttttgcgctg
     1381 actatcggcg atccgaccgg gcgtttgccg aggcgccgat tcggctgcgg gagcgactca
     1441 aagtcccacg gtcagcgggc gtgccgatcg agccgcgagg gatcctcgcg ttgacaccgc
     1501 cttgagatta ctcgcaggac ccctgcgatt acaggttctt gggcttcgcc caccacggca
     1561 cgagtaggga caccgctacg gcggtgatgt agtgtgtcat cgtatccgca aggagatact
     1621 gcgacggaaa gtgccgcgtg cctaagtgtc cgacccccgc gagggccagg agttgccgta
     1681 ccggcacaat gagccccacg atgggaatcc cgattgacgg atttggccct aatgcaaacg
     1741 gcagggtcgc aatgagcaag ctgtcaatcg tcgcaatcgc cagggtgatc acccagagcg
     1801 ccacgatcgg acgacgcacg gctcgcccta accagccgta gagaatgccg ccaagaatgc
     1861 cagcaacaaa tgcggaccag agggtcgcaa cacgaggcgg caattgcgcg cgcatgatga
     1921 tctctgcgat tacactcgcc agtgccgcga ccacaccggc acccgcgccc gcaagaa
//
LOCUS       DASQGH010000378         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52605, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000378 DASQGH010000000
VERSION     DASQGH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52605"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..775
                     /gene="lexA"
                     /locus_tag="VEW91_07380"
     CDS             143..775
                     /gene="lexA"
                     /locus_tag="VEW91_07380"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYM91436.1"
                     /translation="MAQRKLTGRQRQVLEYLVNAVRDKGYAPSIREIGHALGLRSPST
                     VHQHLTALEQKGCVRRHGDRMRALEVVDKTLVQNGASVVLPLVGRVSAGHPILADERV
                     DGYVAVPPRLFGDVRGCFLLRVRGDSMNGAGILPGDVVVVRPQAHAAPGDIVVALLGE
                     DAAVKRLSLIEGRPVLKPENPAYPPIRGEFQVLGRVVGTLRAYDEVAGWA"
     gene            766..1041
                     /locus_tag="VEW91_07385"
     CDS             766..1041
                     /locus_tag="VEW91_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91437.1"
                     /translation="MGVAAARLSRTRTPSPARRAHGAGRRAPGHGSRRIAPGARPRTG
                     HPSERWAPTERTTLWDRLDDDRALMGLKIVLLVSLFLLTALLEWARA"
     gene            1038..1517
                     /locus_tag="VEW91_07390"
     CDS             1038..1517
                     /locus_tag="VEW91_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HYM91438.1"
                     /translation="MSTATVHAYVAAAEPARGRMGMGVVFVDAHGRPLRKVGRALPGG
                     TSRGLGAFRGIVYALWNSRRLGCRQVVVYCPDRDVVAQINGEEDVAEEFVGSYLEVRA
                     LLHAYRAARVECPQGDEDSLWLGLAYELADAAMEGDVDETVEDLPLWSAPTEERSTA"
     gene            1612..>1995
                     /locus_tag="VEW91_07395"
     CDS             1612..>1995
                     /locus_tag="VEW91_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM91439.1"
                     /translation="MSSQDPTTLIAEGEAKLAEGDLDGALGAFQRAAAASPSSAVAHS
                     KLGIVYVHKQQWNAAAGEFGRAIQLDPTYAPAYSNLGNVHRERGELEQAVAHYQKAVV
                     MDPDYWIAHQNLGIVYKQQGRLPEAV"
BASE COUNT          335 a          647 c          708 g          305 t
ORIGIN      
        1 acggcgtccg aacgagaaag ttctaagcga agcggccgtc ccgatagttg cgtacatatg
       61 ttcgccattg cctacctttg tatgcgtact aatgttcggt ataattgatt acgagagcaa
      121 atccgacgaa ggggtcaggt gcgtggccca gcgcaagctt acgggacgcc agcggcaggt
      181 gctcgagtac ctcgtcaacg cggtgaggga caaggggtac gcgccttcga tcagggagat
      241 cggccatgcc ctcggcctcc gctcgccatc caccgtccac cagcatctga cggcgctcga
      301 gcagaaaggc tgtgtcaggc gtcacgggga tcggatgagg gcgctggaag tcgtcgacaa
      361 aacattggtc cagaacggag cgagcgtcgt cctccctctc gtcggtcgag tctcggcggg
      421 ccaccccatc ctcgccgatg agcgggtgga cgggtacgtc gcggtccccc cccggctctt
      481 cggagacgtg cgggggtgct tcctgcttcg cgtccgcggg gacagcatga acggcgccgg
      541 gatcctaccg ggagatgtcg ttgtcgtgcg cccccaggcc cacgctgcac cgggggacat
      601 cgtcgtcgcg ctgctcggcg aggacgccgc ggtcaagcgc ctttccctca tcgagggaag
      661 gcccgtgctc aagcccgaga atcctgcgta cccgccgatc cgcggggagt tccaggtgct
      721 cggccgcgtg gtgggaacgc ttcgggccta cgacgaggtg gccggatggg cgtagccgcc
      781 gcccgactgt ccaggaccag gacgccgagt cccgctcggc gcgcgcacgg agcggggcgg
      841 cgcgccccgg gacacggctc gcgaaggatc gccccggggg cacgcccccg gaccggacac
      901 ccgagcgagc gatgggcgcc gaccgagcgg acgacgctct gggaccggct ggacgatgac
      961 cgcgcgctca tgggcctgaa gatcgtgctc ctggtctcgc tgtttctgct cacagccctg
     1021 ctggagtggg cgcgcgcatg agcaccgcca ccgtacacgc gtatgtcgcc gcggcagagc
     1081 cggcacgcgg ccggatgggc atgggggtcg tcttcgtgga cgcgcatggg cggccgcttc
     1141 ggaaggtcgg aagggcgctg cctggaggga cgagccgggg acttggtgcg ttccgcggga
     1201 tcgtctacgc gctctggaac tcccgtcggc tcggctgccg ccaggtggtc gtgtactgcc
     1261 ccgatcggga tgtcgtcgcg cagatcaacg gcgaagagga cgtcgccgag gagttcgtgg
     1321 ggtcctatct cgaggtgcgg gcacttctcc acgcctaccg ggctgcgcgg gtagagtgcc
     1381 cccaggggga cgaggactcg ctctggctgg ggctggcata cgagctcgcg gacgcggcga
     1441 tggaaggcga cgtggacgag acggtcgagg acctgccgct ctggtccgcg cccacggaag
     1501 aacgctcgac cgcttaaaga tcgctgccgt agggaaaagg aacctccgga tccccccaag
     1561 aatcctgtgc cgccgcaccg cgacaatggc ggccgatcgc tcgtgccgga gatgagttcc
     1621 caagacccca cgacgctgat tgccgaaggc gaggcgaagc tggccgaggg agacctggac
     1681 ggcgctctgg gggcgttcca gcgcgccgcg gccgcgtccc cttcctcggc ggtggcccac
     1741 agcaagctgg ggatcgtcta cgtgcacaag caacagtgga acgccgccgc cggcgagttc
     1801 ggccgcgcga tccagctgga ccccacctac gcgccggcat acagcaacct cggaaacgtg
     1861 caccgggagc gcggtgagct cgaacaggcg gtcgcgcact atcagaaggc cgtggtcatg
     1921 gatcccgatt actggatcgc gcatcagaac ctgggcatcg tatacaagca gcagggtcgt
     1981 ctccccgaag cggtc
//
LOCUS       DASQGH010000379         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_32888, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000379 DASQGH010000000
VERSION     DASQGH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32888"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VEW91_07400"
     CDS             <1..526
                     /locus_tag="VEW91_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91440.1"
                     /translation="RKIVREAGGEQVVTEKVATRLRSLLQEPHVLNPRYTRPKPDNYV
                     LYPVWVEPGGSFSIAAAVWNVGQVTPVHDHGTWGVIGIYQGIEREIRYKPAVVGGERR
                     FKETEERDIGERQVIVCCTSDQDIHRVSCGSNRPCVGIHVYGADIGAIQRHVYEPQTG
                     DVRAFVSGWTSVEE"
     gene            543..>1913
                     /locus_tag="VEW91_07405"
     CDS             543..>1913
                     /locus_tag="VEW91_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYM91441.1"
                     /translation="MLDRAALDTLKASLRGQLIEPGDAVYETARKVYNAMIDRRPRLI
                     ARCADVSDVITALRFGREHSLRVAVRGGGHNAGGLGVCDDGLVIDLAPMRYTRVDPAD
                     RTVRVGGGAVWGDVDHATHAFGMATPSGVISTTGVGGLTLGGGLGHLTRKCGLSIDNL
                     LGADVVLADGSFVTAGAKEHPDLFWAIRGGGGNFGIVTSFLFRLHPISTVHAGPMLWE
                     LDKSTEVMQWYREFITTAPDDISGFFAFLTVPPVPIFPEHLHMKKMCGVVWCYTGPAN
                     RFEEHFKPVRRFRPPALELVGPMPQPALQSMFDGLYPPGHQWYWRADFVKELSDKAIG
                     QHVKYGATLPTMQSTMHLYPVNGAAHRVGKNDTPWAYRDATWAEVMVGVDPDPANKDR
                     ITRWAKEYWDAVHPYSEAGAYVNFMMDEGEDRVKATYRDNYARLAAIKKTYDPTNVFR
                     VNQNIKP"
BASE COUNT          364 a          593 c          635 g          321 t
ORIGIN      
        1 caggaagatc gtgagggaag ccgggggtga gcaggtggtc accgagaaag tcgccacccg
       61 acttcgctcg ttgctgcagg agcctcacgt tctgaatccc agatacacgc gccccaaacc
      121 cgacaactac gtcctctacc cggtctgggt ggagccgggc ggcagttttt ccatcgccgc
      181 agccgtatgg aatgtgggtc aggtcactcc ggttcacgac cacgggacgt ggggagtgat
      241 cggcatctat cagggtatcg aacgtgagat tcggtataag ccggcagttg tgggcggcga
      301 gcggcgcttt aaggaaactg aagagagaga tattggagaa cggcaggtga tcgtctgttg
      361 cacctccgac caagacattc accgggtcag ctgcggatcg aacaggccct gcgtcgggat
      421 tcacgtctat ggcgccgata ttggtgccat tcagcgacac gtctatgagc cacaaaccgg
      481 cgacgtgcgc gctttcgttt cgggctggac ttctgtggaa gagtaaggca gaggaggata
      541 cgatgctgga tcgagcagcc ttggacaccc tgaaagccag cctgcgcggg cagctcatcg
      601 agcccggcga tgccgtctac gagacggccc gcaaggtcta caacgctatg atcgaccggc
      661 gtccccgcct gattgcgcgc tgcgcggacg tgagcgacgt tatcaccgcg ctcaggttcg
      721 gtcgcgagca cagcctgcgg gtggccgttc gaggcggcgg ccacaacgcc ggcggtctgg
      781 gcgtctgcga tgacggcctg gtgatcgacc tcgctcccat gcggtacacg cgcgtcgatc
      841 cagcggaccg caccgtgcgg gtcgggggtg gcgccgtctg gggcgacgtg gaccatgcca
      901 cgcatgcctt cgggatggcc acgccgagcg gcgtcatctc gaccacggga gtgggaggtc
      961 tcaccctcgg cggagggctc ggccatctga cgcgcaagtg cgggctctcc atagacaacc
     1021 tgctcggcgc cgacgtggtg ctggccgacg gcagcttcgt gaccgccggc gcgaaagagc
     1081 accccgatct gttctgggcc atccgcggcg gcggtggcaa cttcggcatc gtgacgtcgt
     1141 tcctgttcag gctacatccg atcagcacgg tccatgcggg accgatgctg tgggagctcg
     1201 acaagtcgac cgaagtgatg cagtggtacc gcgagttcat caccaccgcc ccggacgaca
     1261 tcagcggatt cttcgccttt ctgaccgtgc cgcccgtgcc gatcttccca gagcacctgc
     1321 acatgaagaa gatgtgcggg gtcgtgtggt gctatacggg acccgcgaac cgcttcgagg
     1381 agcacttcaa gccggtccgc cggttcaggc cccccgcgct cgagctggtg ggccccatgc
     1441 cgcagccggc cctccagagc atgttcgacg ggctgtaccc gcccggccac cagtggtatt
     1501 ggcgggcgga cttcgtcaag gagttgagcg acaaggccat cggacagcac gtgaagtacg
     1561 gggcgaccct cccgactatg cagtccacca tgcacctcta tccggtcaac ggggcggcac
     1621 accgggtggg caagaacgat acgccctggg cctatcgcga tgccacgtgg gccgaggtca
     1681 tggtcggggt ggatcccgac ccggcgaaca aagaccgcat caccagatgg gcaaaggagt
     1741 actgggatgc ggtccatccc tactcggagg ccggggcgta cgtgaacttc atgatggacg
     1801 agggcgagga tcgcgtgaag gcgacctacc gcgacaacta cgcacgcctg gccgccatca
     1861 agaagacgta cgacccgacc aacgtgttcc gcgtgaacca gaacatcaag ccg
//
LOCUS       DASQGH010000380         5798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47003, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000380 DASQGH010000000
VERSION     DASQGH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47003"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..846)
                     /locus_tag="VEW91_07410"
     CDS             complement(82..846)
                     /locus_tag="VEW91_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HYM91442.1"
                     /translation="MARPAGVMALEVAGATDPGRIRPKNEDYLRYEVPADPLLGEARG
                     SLFLVADGVGGAGGGEVASAEAAHTVMQEYYLDLRGGPGRTLRRAIERANLHVYHLRR
                     GHPGFSGMQTTMTALVLCGGRYWLGHVGDSKAFLLRGGIFRQLTRDHTFVQEMRRLGL
                     VDSRRADRHPHRHLLTRTIGGDPLVAIDLQRGPARAGDCFVLATDGLFEHLGPEDVRE
                     AFANSPPDAAAGACVEEAKRRGGFDNLTVLAVRIPG"
     gene            complement(868..1479)
                     /locus_tag="VEW91_07415"
     CDS             complement(868..1479)
                     /locus_tag="VEW91_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYM91443.1"
                     /translation="MADRGDGRVLLVDDEALIRMGMRVMLQDLGYEVVGEAADGQEAI
                     EQVDALDPDVVIMDIKMPGMDGLEATRRIMAAHPVPIIVLTAYNQRSLVEEAADAGVL
                     AYLMKPVRESDIAPAIEVARARFADLRWLRRTPGDTPGARAAIRPPQDEGANPRVETA
                     TRILRARYRLTEAQAAERLRRLARSSRRTVGEVAEAIVALEGE"
     gene            1861..3396
                     /locus_tag="VEW91_07420"
     CDS             1861..3396
                     /locus_tag="VEW91_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYM91444.1"
                     /translation="MSNGTGFRKSLTLTGVTVNAMALIAPGAFLWITYQLQAAQVDPA
                     GASTALDIWPGLALALILAFLTAASYSMLSELYPDAGTGSSYYFAEKAFLDKEEHDYH
                     RWARMAKYVVGWISHLYYWVYPGVMVAMMATMIIYVLGLFNVNLPPVAQVAIAVGFSF
                     VTAYVAYRGITGSTLANLLVNAIQIAALVVISVLALSYRFANPQHVTFLHPTLASAVL
                     PHNLSHVLFQSTIAILLLVGFESATALSAEALHPRFVGRGVILSLIIQGLIAYLFEYF
                     AAGAWINTAYTLKDAKGKVLTGFDAAGFSQAPIGDMVKNVGDVMLGGIGFPLLLIIAV
                     TVAIAVFGTTLACMNTGVRLTYAMGRDEEVPAILGVLHGRFATPHFGVWLMAAASAVI
                     GGFGVLSVLNLTAITFLSNIGTFLLYGLTNIVAFVAFRRAPRAHTAKHVVVPLLGAFA
                     NLAMLLAVIYLGILGGGDTRTAALISIIAAGAWFVAGAVYFVGNTRTRGRPVWAEKGV
                     GTA"
     gene            complement(3436..4125)
                     /locus_tag="VEW91_07425"
     CDS             complement(3436..4125)
                     /locus_tag="VEW91_07425"
                     /inference="COORDINATES: protein motif:HMM:NF022463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HYM91445.1"
                     /translation="MHLELEVAASQGVWRWTPRVRAVLNAGWAGRDRAAVAAHIEEMR
                     ALGVPAPEQVPIVFPVARAMLTQGDRIEIYSPQSSGEVEYVLIASRERVALTVGSDHT
                     DRRVEATSIELSKQICPNVLARTAWDYADLAPRVDDLILRAWVRTGGTWERYQEAPIG
                     ALLAPAYWLGRLTGRLTEGAAVVLFSGTVPTLGGAVRYGDGFRISLEDPRLGRSIVHE
                     YDVALVENPLA"
     gene            complement(4132..5385)
                     /locus_tag="VEW91_07430"
     CDS             complement(4132..5385)
                     /locus_tag="VEW91_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM91446.1"
                     /translation="MTGEGNAGRTRLPVLAPLRHRNLRLFFAGQGVSLIGTWMQTVGQ
                     VWLVLILTHSPFLLGVINALQWAPVLLFSLPAGVVVDRVPKRALIMSTQTLFLLLALG
                     LGLLTIIGHVRYWHVAVLAALLGTVNAFDVPARQAFIVEMVGGTEDLTGAIALNSAVF
                     NGARLVGPALAGLIIAAWGVGVAFLANAVSFLAVLAALGAMRVVSTIRVAPGIGWLAH
                     VAEGVAFIRSTTTVLKVLVTLGVLSLFAMNFNVFVPVLARSQLHLGASGFGFLMAAQG
                     LGALIGSLIVAGTSARGPRPPHLRWGAVLLCMALLALSRVGRADVAGVELFLAGMGMV
                     LFTATANSTVQLETPHELRGRVMSVYALVFSGLAPFGALMMGGIIGAWGLPAGLIVGG
                     GVGLAGTLAVWGFLRPRGSPAAVPG"
     gene            complement(5382..5798)
                     /locus_tag="VEW91_07435"
                     /pseudo
     CDS             complement(5382..5798)
                     /locus_tag="VEW91_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006699774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          902 a         2136 c         1927 g          833 t
ORIGIN      
        1 ccctcgcccc agcgggcgct ctacgagata gtgaccaacg cgatcaccgg gggctggcat
       61 gagtggagtg acaagatgcg ctcagccggg gatccggacc gcgagaacgg tcaggttgtc
      121 gaatccacca cgccgtttcg cttcctcaac gcaggcgccg gctgcagcat ccgggggaga
      181 gttcgcgaac gcctcgcgaa cgtcctccgg cccgaggtgc tcgaacaggc cgtctgtggc
      241 cagcacgaaa caatccccgg cacgggccgg accccgctga aggtcgatgg ccacgagcgg
      301 atccccaccg atggtccgcg tcaggaggtg gcggtgcgga tggcgatcgg cgcggcgtga
      361 atccaccagg ccgaggcggc gcatctcctg gacgaatgtg tgatcccggg tcaactgccg
      421 aaagatcccc ccgcgcagca ggaacgcctt ggagtctccc acgtgtccca gccagtaccg
      481 cccgccgcag agcacgagcg cggtcatcgt ggtctgcatc cccgagaatc ccggatgccc
      541 ccggcgcagg tggtagacgt ggaggttggc ccgctcgatc gcccgccgga gggtccgacc
      601 gggaccgccg cggaggtcaa ggtagtattc ctgcataacc gtgtgcgcgg cctcggcgct
      661 cgccacctcg cctccgccgg cacctcccac accgtcggcc accagaaaca ggcttccccg
      721 ggcctccccg agcaacgggt cggcgggcac ctcgtagcga aggtagtctt cgttcttcgg
      781 ccggatcctc cccggatccg tggcgccggc gacctcgagc gccatcaccc cggcggggcg
      841 agccaccctc ggcatccgca tcgggcccta ctcgccctcc agcgcgacga tcgcctcggc
      901 aacctccccc accgtcctcc gcgaactccg cgcgaggcgc cgcaaccgct cggccgcctg
      961 cgcctcggtc agccggtagc gggcgcgcaa gatccgcgtg gccgtctcga cccggggatt
     1021 cgccccctcg tcttgggggg gacggatcgc tgcccgcgcc ccgggggtgt cgccgggcgt
     1081 ccggcggagc cagcggagat cggcgaaccg ggcccgggcc acctcgatcg ccggtgcgat
     1141 gtccgattcc cggacgggct tcatcaggta cgcgagcacc ccggcgtcgg cagcctcctc
     1201 caccaggctc cgctggttgt acgcggtcag gacgatgatc ggcaccgggt gcgccgccat
     1261 gatcctgcgc gtcgcctcca acccgtccat cccaggcatc ttgatgtcca tgatcacgac
     1321 gtccggatcc agcgcatcga cttgctcgat ggcctcctgt ccgtcggcgg cttcgcccac
     1381 gacctcgtag ccgaggtcct gaagcatcac gcgcatcccc atccggatga gcgcttcgtc
     1441 gtccacaagc agcacgcgtc catctccgcg gtcggccatc ccggtcctcc ccccggcgaa
     1501 cacgcaccgg tcccccttgg tgccgccggg tgaaaataca gatacccccg cccggtcgag
     1561 cgggggcatc gcggcccagg gccatgctat cacgaccccc cgaggagatt caagagcggc
     1621 ggccgggttg acagacgggg cggcgtctgg tatatgtagc ccagcaccac gacgtgcgtg
     1681 aggccaggac gccgacctgc tttcgcggtc ggcgttgcta ttttccggcc cgtgggcgac
     1741 gacgcacccg cctgcccccc tttccccccg ttcgggggac aggcggggga caggccgaga
     1801 ctgccccgcc gcagtgaccc gccgggtcgc gaccccgatg gacaaaagga ggccgcgcgc
     1861 atgtcgaacg gcacaggatt tcgaaaaagc ctgacgctca cgggcgtcac ggtgaacgcc
     1921 atggcgctca tcgcgcccgg ggcattcctc tggatcacgt accagctcca ggccgcccag
     1981 gtggaccccg cgggcgcctc gaccgcgctc gacatctggc ccgggctcgc cctcgcgctc
     2041 atcctcgcct tcctcaccgc ggcgtcctac tcgatgctca gcgagctcta cccggacgcc
     2101 gggacgggat cgtcgtacta cttcgcggaa aaggcgttcc tcgacaagga agaacacgat
     2161 taccaccggt gggcccggat ggcgaagtac gtcgtcgggt ggatctcgca tctctactac
     2221 tgggtctatc ccggcgtcat ggtcgccatg atggcgacga tgatcatcta cgtcctgggg
     2281 ctcttcaacg tcaacctccc gccggtcgcg caggtggcga tcgcggtggg gttctccttc
     2341 gtcacggcct acgtggcgta ccgggggatc accggctcca ccctcgcgaa cctgctcgtc
     2401 aacgcgatcc agatcgccgc gctggtggtg atctccgtgc tagcgctgag ctaccgcttc
     2461 gccaacccgc agcatgtgac gtttctgcac ccgacgctgg ccagcgccgt gctcccgcac
     2521 aacctgagcc acgtgctgtt ccagtccacg atcgcgatcc tgctgctcgt ggggttcgag
     2581 tcggccacgg cgctttcggc cgaggcgctc cacccccggt tcgtcggccg cggcgtcatc
     2641 ctctcgttga tcatccaggg cctgatcgcc tatctcttcg agtactttgc ggccggcgcc
     2701 tggatcaaca ccgcgtacac gctgaaggac gccaagggga aggtgctgac ggggtttgac
     2761 gcagccggct tctcccaggc cccgatcggc gatatggtga agaacgtggg cgacgtcatg
     2821 ctgggcggca tcggatttcc gctcctgctc atcatcgcgg tgacggtcgc gatcgcggtg
     2881 ttcggcacga cgctcgcctg catgaacacc ggcgtccgcc tgacctacgc gatgggacgc
     2941 gatgaagaag tgccggcgat cctcggggtc ctgcacgggc gcttcgccac cccgcatttt
     3001 ggcgtgtggc tcatggcggc cgcctccgcg gtcatcggcg ggttcggggt gctctccgtc
     3061 ttgaatctga ccgccatcac gttcctgtcg aacattggga cattcctact gtacgggctg
     3121 accaacatcg tggccttcgt cgcgttccgc cgggcgcccc gggcgcatac cgcaaaacac
     3181 gtggtggtcc cgctcctcgg cgcgttcgcc aacctggcga tgctgctggc ggtcatctac
     3241 ctcgggatcc tgggcggcgg ggatacccgg accgcggcgc tgatctcgat tatcgccgcc
     3301 ggcgcctggt tcgtggccgg ggccgtgtac tttgtcggca acacgcggac gcgcggccgg
     3361 ccggtctggg ccgagaaggg agtcggcacc gcctagccgc actgcacagg ggggaccccc
     3421 cgccggggac ggccgtcagg ccagcggatt ctccacgagc gccacgtcgt actcgtggac
     3481 gatcgagcgc ccgagccggg ggtcctccag gctgatccgg aaaccgtcgc cgtaccggac
     3541 ggcgccgccg agggtcggca ccgtgccgga gaacagcacc acggccgccc cctcggtgag
     3601 ccgtcccgtg aggcggccga gccagtacgc cggggcgagc agcgcgccga tcggggcctc
     3661 ttgatagcgc tcccaggtgc ccccggtccg gacccatgcc cggaggatga ggtcatcgac
     3721 ccggggggcg aggtccgcgt agtcccaggc cgtccgcgcg aggacgttgg ggcagatctg
     3781 cttggacagc tcgatcgagg tggcctccac ccgccggtcc gtgtggtcgc tcccgacggt
     3841 caacgcgacc cgctcccggc tggcgatgag cacatactcc acttcgcccg agctctgggg
     3901 cgagtagatc tcgatccggt ccccctgggt gagcatcgcg cgggcgaccg gaaacacgat
     3961 cgggacctgc tcgggggcgg gcacccctaa cgcccgcatc tcctcgatgt gggcggccac
     4021 cgccgctcga tcccggccgg cccaccccgc gttcaggacg gcccggaccc ggggcgtcca
     4081 ccgccagaca ccctgtgatg cggcgacctc gagctccagg tgcatcgccg cctaccccgg
     4141 aaccgccgcg ggggacccgc ggggcctgag gaacccccaa accgccagcg tgcccgcgag
     4201 ccccaccccg ccccccacga tcaaccccgc cggcaacccc caggcgccga tgatgccgcc
     4261 catcatcaac gccccgaagg gcgccaggcc gctgaacacg agggcgtaga cgctcatcac
     4321 ccgcccccgc agttcgtggg gggtctcgag ttgtaccgtg ctgttcgcgg tggcggtgaa
     4381 caacaccatc cccatccccg ccaggaagag ctcgaccccg gccacatccg cgcggccgac
     4441 ccggctgagc gccagcaggg ccatgcagag cagcacggcg ccccagcgca ggtgcggggg
     4501 gcgcgggccg cgggcgcttg tgccggccac gatcagcgac ccgatgagcg ccccgaggcc
     4561 ctgggcggcc atgaggaacc cgaaaccgct cgccccgagg tggagctggc tgcgcgccag
     4621 caccggcacg aacacgttaa agttcatggc gaacaggctt agcaccccga gcgtgaccag
     4681 gaccttgaga accgtcgtgg tcgaccggat gaacgccacg ccctcggcga cgtgcgcgag
     4741 ccagccgatt cccggtgcga ctcggatcgt ggacacgacc cgcatggcgc cgagcgccgc
     4801 gagcacggcg aggaaactca ccgcattcgc cagaaacgcc acgcccacac cccaggcggc
     4861 gatgatcagg ccggccagcg ccggcccgac gaggcgcgcc ccgttgaaca ccgcggagtt
     4921 gagggcaatc gcgcccgtca ggtcctcggt ccccccgacc atctccacga tgaacgcctg
     4981 ccgcgccggc acgtcgaagg cattgaccgt ccccagcagc gcggccagga ccgccacgtg
     5041 ccagtaccgg acgtgcccga tgatggtgag caggcccagg ccgagggcga ggaggaggaa
     5101 cagcgtctgg gtgctcatga tcagggcccg cttcggcacc cgatccacca cgacgcccgc
     5161 cgggagcgag aacaggagca ccggggccca ttggagcgcg ttgatcacgc cgaggaggaa
     5221 cggcgaatgc gtgaggatca gcacgagcca cacctgcccc accgtctgca tccaggtgcc
     5281 gatgagcgag accccctggc ccgcgaagaa aagcctgagg ttgcggtgcc gcagcggggc
     5341 gagcacgggg aggcgggtgc gacccgcgtt cccctccccc gtcacccggg ccaaaggccg
     5401 agccgttgcg cgcccgcgac gagggtacgg gtatacggat gctggggcgc ggcaaacagc
     5461 tggcgcgttc cggccgattc gacgatccgc ccggcgcgca tcacgtggac caactcgcac
     5521 gtctgggcca cgaccccagg tcgtgggtga tcagcagcac cgacatcccg agctcgcgcc
     5581 ggaggcggcc gaggaggtcg aggatctgcg cctgcacggt gacgtccagg ttggcggtcg
     5641 gctcgtcggc gatcaggagc tccggcccgg gggcgatcgc cccggcgatg agcacgcgct
     5701 gccgctggcc tcccgacagc tcgtgcgggt agcgcttgag cagcgaggga tccagcccgc
     5761 cgcgctgcag ggcctcggcg gcccgctccc gcgcgccc
//
LOCUS       DASQGH010000381         8207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21353, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000381 DASQGH010000000
VERSION     DASQGH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21353"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VEW91_07440"
     CDS             <1..343
                     /locus_tag="VEW91_07440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYM91447.1"
                     /translation="SGYGWRTHPIFGTREFHTGIDIAAPYGTPVQAAHDGGVIFNGWM
                     RGYGLLVILDHGGGLTTTYSHLSASLVHVGDRVVRGAVIARIGSTGWSTGPHLFFEVR
                     EDGRPIDPAGE"
     gene            605..886
                     /locus_tag="VEW91_07445"
     CDS             605..886
                     /locus_tag="VEW91_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HYM91448.1"
                     /translation="MAQVYLPMPLRRLTRGQARVVAGGRTVEEALVDLEQRYPGLREQ
                     LREPTGEVRRFINVFVNGTEIRMLQGPATPVGEGDEVSIVPAMAGGMGP"
     gene            889..1779
                     /locus_tag="VEW91_07450"
     CDS             889..1779
                     /locus_tag="VEW91_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91449.1"
                     /translation="MDLFTRQRVPSTARVTPVLRVLETSQLLLHEDPDLERVKRLAEA
                     FRRDGALRNPPIVTPLDLPRPATQAGEGAAPMVVLDGANRVTALREIGVPHVAVQVVQ
                     YERPEIVVSTWRHYVVEAEGPPLRERIADRLGIGVAPLAGSAEAEDRLGRREGVAALV
                     DGRGVALVGQGADPNAHAGLLSGLVGLYQGTDALYRIDREDLKALRAEHGRGTLVIFP
                     PLGKADILQLATTGGRLPAGVTRHLVPGRVLRLNVPLEWLQRSEPTAAKQRRLDAAVE
                     ERWRAHGIRYYAEATYLFDE"
     gene            1733..2713
                     /locus_tag="VEW91_07455"
     CDS             1733..2713
                     /locus_tag="VEW91_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HYM91450.1"
                     /translation="MGSGTTQRRPISSTSKRLCVRPASGTSLLDQIGRTPLVRLDRVA
                     RGLPRAVHVYIKAEWFNPGGSVKDRPVLRMITEAERDGRLTPGKTILDSTSGNAGIAY
                     AMIGAIKGYPVELVMPASASAERKRIIAAYGARIVLSDPLEGSDGAIRVARAMLGAAP
                     ERYFKPDQYNNPANWQAHYDTTGVEILEQTEGRITHFVAGLGTTGTVVGAGRRLHEAD
                     PGIRVIAVEPDAALHGLEGLKHIASSIVPGIYDPSVHDEKLGVATEAGYAMARRLARE
                     EGLFVGASTGAAVHAALEVAARLRVGVVVAIAPDGGDRYLSTPLWRVGER"
     gene            2768..3190
                     /locus_tag="VEW91_07460"
     CDS             2768..3190
                     /locus_tag="VEW91_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HYM91451.1"
                     /translation="MTLRVRRDQLDRIIAQARAEAPNECCGMLAGRGGVVEEVFPGRN
                     QDQSPKTYLMDPQDQLRAFRAMDEQGWDLVGIYHSHPHTEAYPSRTDQERAVDREGSP
                     LFPEARYMIVSLRDAARPQVRAFRITDGQVTEEDVVVS"
     gene            3187..3615
                     /locus_tag="VEW91_07465"
     CDS             3187..3615
                     /locus_tag="VEW91_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYM91452.1"
                     /translation="MKVSTRAEYGIRALMDLAQYYGQGPVQSHDIARRQGLPEPYLNQ
                     LLVTLRRAGLVQSKRGPAGGHLLTRSPDRITVGEAFLALEGSIAPWLCVEEAETTCIY
                     APGCGLRPVWQAVKEATEQVLNRTTLADIVRQMLVPLGKP"
     gene            3747..4451
                     /locus_tag="VEW91_07470"
     CDS             3747..4451
                     /locus_tag="VEW91_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM91453.1"
                     /translation="MGEAETPESTEPGQAEAQEADAVGAGVPLGGGPEGESALATPAG
                     PEARARFEQLIAEHLDGLYHSARRLTRNPVAAEDLVQDVMLKAWRSFHTFQDGTSIRA
                     WLHRILMNAFFDRYRKQAREPEVVDQQGVDDFYLYDKAREGAALGEAGDPAVEVLDRI
                     MDAEVRESVESLPMPYRAAVVLADVQGFTYKEIAEMLGIPVGTVMSRLFRGRHLLQRQ
                     LWEYARQRHLLKGDGR"
     gene            4448..4672
                     /gene="rsrA"
                     /locus_tag="VEW91_07475"
     CDS             4448..4672
                     /gene="rsrA"
                     /locus_tag="VEW91_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HYM91454.1"
                     /translation="MNCEETLEKLWQFLDKELDGESSGELQRHLEECRHCFSKAGFEQ
                     RLRAIVRRSCSGEQAPPELRERLRKLIRLF"
     gene            4782..5690
                     /locus_tag="VEW91_07480"
     CDS             4782..5690
                     /locus_tag="VEW91_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91455.1"
                     /translation="MLRRLRITRLGERLTPGGRIQPAAAARTAQAVKNFIALAAAAGA
                     APPVLVGTHALRAARNPDELLARLDRPVRILTGDQEARLGYRGVLAGLGPPPAASRLF
                     VVDIGGGSIELTWGSRGRIARSLSIPAGAVVLTERFLAHDPPLAAEIGALEAHLARTL
                     GPGLPRVSGRPLRLVGVGGTITTMAAIEQRLSPYDPDRVHSFRLSRRAIERITADLLA
                     CPLAERRRLPGLQPERADIIAAGALVLRYLMVRYGARSITVSEADLLWGIVLEARAGI
                     SRRKSRFRPNRFTRASALTPRESMLQ"
     gene            5729..5805
                     /locus_tag="VEW91_07485"
     tRNA            5729..5805
                     /locus_tag="VEW91_07485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5763..5765,aa:Met,seq:cat)
     gene            6688..7206
                     /locus_tag="VEW91_07490"
                     /pseudo
     CDS             6688..7206
                     /locus_tag="VEW91_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7621..>8207)
                     /locus_tag="VEW91_07495"
     CDS             complement(7621..>8207)
                     /locus_tag="VEW91_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM91456.1"
                     /translation="PLVIHPEAWRERKIIFPTGTELRLPPPSRHDLEEEGLQLIEERG
                     QTLLLDDTVLISGQVERTTDFEKGFPIHYARTQAGWTPDPMIWDDQNVIVNVRGKGLV
                     IVSGCSHAGAVNVLRNAQQLTGEKRIAGFIGGFHLTGGIFEAIIDPTVKAFIDAEVGR
                     VLPAHCTGWKAVHALARAMPSAFVQPAVGTIVSF"
BASE COUNT         1321 a         2692 c         2802 g         1392 t
ORIGIN      
        1 ctcggggtac gggtggcgca cccacccgat ctttgggacc cgcgagttcc ataccggcat
       61 cgacatcgcc gccccgtacg gcacgcctgt ccaggccgcc cacgacgggg gcgtcatctt
      121 caacgggtgg atgcgcggct acgggttgct ggtgatcctg gaccacggag gcgggctcac
      181 gaccacctac tcccacctct cggcctcgct ggtgcatgtc ggcgatcgag tcgtccgggg
      241 cgccgtcatc gcgcgcatcg ggagcaccgg gtggagcacc ggcccgcatc tgttcttcga
      301 ggtccgggag gacgggcgtc cgatcgaccc cgcgggagag taaggccgac cgccgccttg
      361 actgcccggg gctcccttgt tagactccgc ctgaaagcgg tggattccgg gaatgtcctg
      421 gggggagaag gcgttccccg aacgagatga tcacctgcat ctgtacgacc ggacaaagcg
      481 gggcgacgga gggatgtcgc cgggcataag cgcgggcgtc cggccctgac aggtgcacac
      541 cgagcatcga agccgcggcg cccgtcaccg cggcgtttgt gttttctcga ggaggtggca
      601 ctgcatggca caggtctatc tgccgatgcc cctgcgccgg ttaacccggg gacaggcccg
      661 ggtggtggcg ggcgggcgca ctgtggagga agcgctggtc gaccttgagc agcggtatcc
      721 ggggttgcgc gagcaactgc gcgagccgac cggggaggtc cgccggttca tcaacgtgtt
      781 cgtcaacgga accgagatcc ggatgcttca ggggcccgcc acgccggtcg gcgagggcga
      841 cgaggtgtcg atcgtcccgg ccatggctgg ggggatgggt ccgtaggcat ggatctgttt
      901 acgcggcagc gcgttccttc gacggctcgg gtgacgcccg tcctccgcgt gctcgagacg
      961 tcgcagttgc tgctgcacga ggatcccgac ctcgagcgcg tcaagcgcct ggccgaggcg
     1021 ttccggcgcg atggcgcgct tcgcaatccc ccgatcgtga ccccactcga cctgccgcgg
     1081 ccggcgacac aggcgggcga gggtgccgcg ccgatggtgg tcctggacgg cgccaatcgc
     1141 gtgaccgcgc tccgcgagat cggggttccg cacgtcgccg tccaggtcgt ccaatacgaa
     1201 cggcccgaga tcgtggtgtc aacctggcgg cactacgtcg tggaggccga ggggccgccg
     1261 ctgcgggagc ggatcgccga ccgtctcggg atcggcgtcg ctcccttggc cgggtccgcc
     1321 gaagccgagg accggctggg gcggcgcgag ggcgttgcgg ccctcgtcga tggtcggggg
     1381 gtggcgctcg tcgggcaggg cgccgatccc aacgcgcacg cggggctgct gagcggcctc
     1441 gtcggtttgt atcagggaac ggacgcgctc tatcggatcg accgtgagga tctcaaagcg
     1501 ctgcgcgcgg agcatgggcg gggaaccctg gtgatctttc ctcctctcgg caaagcggac
     1561 atcctgcagc tggccaccac cggcgggcgg ctcccggccg gggtcacccg gcacctcgtc
     1621 ccgggccggg tcctgcgcct caacgtgccg ctcgagtggc tccaaagatc ggagcctacc
     1681 gcggccaagc agcgccgcct cgacgcggcg gtggaggagc ggtggcgcgc gcatgggatc
     1741 cggtactacg cagaggcgac ctatctcttc gacgagtaag cggctctgcg ttcggcccgc
     1801 atctgggacg tcgctcctgg atcagatcgg gcgaaccccg cttgtgcgcc tcgaccgcgt
     1861 cgcgcgcggc ctgccccggg ccgtgcacgt gtacatcaag gccgagtggt tcaaccccgg
     1921 cggctcggtc aaggaccgcc cggtgcttcg gatgatcacc gaggccgagc gggacggccg
     1981 gctcaccccc gggaagacca tcctcgactc gacctcggga aacgccggga tcgcctacgc
     2041 gatgatcggc gcgatcaagg gctacccggt cgagctggtc atgcccgcga gcgcaagcgc
     2101 ggagcgcaag cggatcatcg cggcgtacgg cgccagaatc gtgctctccg acccgctgga
     2161 gggcagcgac ggcgcgatcc gcgtggccag ggcgatgctg ggcgccgcgc ccgagcgcta
     2221 cttcaagcca gaccagtaca acaatcccgc caattggcag gcgcactatg acacgacggg
     2281 cgtggagatc cttgagcaaa ccgaggggcg gattactcac tttgtcgccg ggctcggcac
     2341 gacggggacg gtggtggggg cggggcgccg gctgcacgaa gccgatcccg gcattcgcgt
     2401 gatcgcggtc gagcccgatg cggcgctgca cgggttggaa gggctcaagc acatcgccag
     2461 ttcgatcgtg ccgggcatct acgacccctc cgttcacgac gagaagctcg gcgtggcgac
     2521 cgaagccggg tacgcgatgg cgcgccggct ggcccgggag gaagggttgt ttgtcggagc
     2581 atcgaccggg gccgctgtcc atgcggcgct cgaggtggcc gcgcggcttc gggtcggcgt
     2641 cgtcgtcgcc atcgcccccg atggtggcga tcggtacctg agcactccgc tgtggcgtgt
     2701 cggcgagcgg tagcgggttg ctcttttcag ccgatttccc taaaatggtg ctctaggagc
     2761 agttgtggtg acgctcaggg tcagacggga tcagttggat cgcatcatcg cccaggcccg
     2821 cgctgaggcc ccgaacgagt gctgcgggat gctggcgggc cgcggagggg tggtcgagga
     2881 ggtcttccct ggccggaacc aggatcagag tccgaagacc tatctgatgg atccgcagga
     2941 tcaactgcgc gcgttccggg cgatggatga gcagggatgg gatctcgtgg ggatctatca
     3001 ctcccatccg cacacggagg cgtatccgag ccggaccgat caagagcggg cggtggatcg
     3061 agaggggagt ccgctcttcc cggaggcccg ctacatgatc gtgtcgctgc gcgacgcagc
     3121 gcgcccgcag gtcagagcct tccggatcac ggacggccag gtcacagagg aggacgtggt
     3181 ggtctcgtga aggtcagcac ccgcgccgaa tacgggatcc gtgcgctgat ggatctggcc
     3241 cagtactacg gccaggggcc cgtgcagagc cacgatatcg ccaggcgcca gggcctcccg
     3301 gagccgtacc tcaatcaact gttggtgacg ctgcggcggg cgggactcgt gcagagcaag
     3361 cgcggccccg ccggcggcca cctcctcacc cggtcgcccg accggatcac cgtaggggag
     3421 gcgtttctcg cgctcgaagg cagcatcgcg ccgtggctgt gcgtggagga ggcggagacc
     3481 acgtgcatct acgccccggg atgcggcttg cgccccgtct ggcaggcggt gaaagaggcc
     3541 accgagcagg tcttgaaccg cacgacgctg gccgacatcg tccgtcagat gctggttccg
     3601 ctgggcaaac cctaacccgg acgaagccgc ggccgccacg agcgcggcca cccacgcaag
     3661 gcccttcttc ggtccttcac ctggaatctt cgcctggcga gcgacgttgc accgactgta
     3721 gggaccctgc gaaggagcgt gttggcatgg gggaagccga gacccccgag tccaccgaac
     3781 ccggacaggc ggaggcccag gaggcggatg ccgtcggtgc cggtgtgccg ttggggggcg
     3841 ggccggaagg cgagtcagcc ctggctacgc cggcaggccc cgaggcgcgc gcccggttcg
     3901 agcagctgat cgccgagcac ctcgacgggc tgtaccactc ggcccgccgg cttactcgca
     3961 acccggtggc ggccgaggat ctggttcagg acgtcatgct caaggcgtgg cggtcgtttc
     4021 acaccttcca ggacgggacc agcatccggg cgtggttgca ccggatcctc atgaacgcct
     4081 tcttcgatag gtaccggaag caggcccggg agccggaggt ggtggatcag cagggtgtcg
     4141 atgacttcta cctctacgac aaggctcgcg agggcgcggc gctcggcgag gcgggcgacc
     4201 ccgcagtcga ggtcctggat cgcatcatgg atgccgaagt gcgtgagagc gtcgagtccc
     4261 tccccatgcc gtatcgggcc gccgtcgtgc tcgccgacgt gcaggggttc acatacaagg
     4321 agatcgccga gatgctcggg atccccgtgg gcaccgtgat gtcccggctg ttccggggcc
     4381 ggcatttgct gcagcgccag ttgtgggagt acgctcggca gcggcacctc ctcaagggag
     4441 acggaagatg aactgtgaag agaccttgga gaaattgtgg cagttcctcg ataaggagct
     4501 cgacggagaa tcctccggcg agcttcagcg gcatctcgag gagtgccgcc attgcttctc
     4561 caaggcgggg tttgagcagc gcctcagggc gatcgtgcga cgatcctgct ccggcgagca
     4621 ggctccgccc gagcttcggg agcgcctgcg caagctgatt cgcctcttct agccctgcgg
     4681 cgcgtgccca ggcaggcggc catcgatatc ggcaccaact cggtgcgcct gctggttgcc
     4741 gaggttccgg acccccctcc cgttcgcctc gcgtttcgcc tgtgcttcgc cgcctgcgga
     4801 tcacccggct cggggagcgt ctcacacccg gaggccggat ccagcccgcc gccgctgcgc
     4861 gcaccgccca ggcggtcaag aacttcatcg ccctggccgc ggcggcgggg gctgcacccc
     4921 ccgttctcgt ggggacgcac gctctccgcg ccgcgcggaa ccccgacgag ttgctcgcgc
     4981 ggctggaccg tccggttcgc atcctcaccg gtgaccagga ggcccggttg ggctaccggg
     5041 gagtcctcgc cggtctcggc ccgcccccgg cggcctcgcg cctcttcgtc gtggacatcg
     5101 gtgggggcag catcgaactc acgtggggga gtcgtgggcg gattgcacga agcctcagca
     5161 tccccgccgg tgccgtggtc ctcaccgagc ggttcctggc gcacgatcca ccgctggccg
     5221 cggagatcgg cgcgctcgaa gcacacctgg cccgcaccct cgggcccggc cttccccggg
     5281 tgtccggccg gccgctccgc ctggtcggtg tggggggaac tatcaccacg atggccgcga
     5341 tcgagcagcg gctttcgccc tacgatccgg accgtgtgca cagcttcagg ctttcacggc
     5401 gggcgatcga gcgcatcacc gcggatctgc tggcgtgccc attggccgag cgtcgccgcc
     5461 tccccggcct ccagccggag cgcgcggaca tcatcgccgc gggtgcgctc gtcctccggt
     5521 acctgatggt gcggtatggt gcccgatcca tcaccgtgag tgaggcagat ctcctctggg
     5581 ggattgtgct cgaggcgcgc gctggcatct cgcgccgcaa atcccggttc cgtccgaatc
     5641 gctttacacg cgcgagcgcg ttgacacccc gggaatcgat gctacaatga aggatgccgg
     5701 tagtgtgatt cctgcaaacg gttgtcgacg cggggtggag cagtccggta gctcgtcggg
     5761 ctcataaccc gaaggtcgct ggttcaaatc cagcccccgc taccatactt aggaatacag
     5821 gggcttgctc atcaaagctg agcaggcccc tgacatttcc ctggagttga gccttcaggc
     5881 cctctggggt gggcttgagg ataatctctc ccagaaggct ccggaggatg gtccgggcct
     5941 cgtccacatc cttcctgagc acacgttcga aagcttgtag tcgtcgctga atgacctccg
     6001 gcagcacagc cagcgccttg atgtcggcct gccggggcat agcaagccgg tcctggagcc
     6061 cccggacctt cgattcggcc tgtgcaatca tctcttgggt cacgttcccg atcagaccgc
     6121 gcttgatcgc catccggata tggtccaact ctgccagggc ttccgtcagg tcagcctcaa
     6181 gccgcgctcg gtcatcctgg ggcatctgac gacggcatat ctgctgttat ccgccgccat
     6241 cactgctggg acggcatcca tcgaggaggt cggtgaaggg agagttccga ccctccggat
     6301 cgtcaaccgc catgtcgagt cgcgggcttg tcgggagggc agaacggatg cccctggggg
     6361 accgcggcag agtccggcct gcgcccaagc tctcctgcgc cgaacgcgcg cggttggagc
     6421 gcgtgctcct caagggccca ttggctgccg gctatgcgac agacctgtgg acgctcaaac
     6481 gcgtcgcggg ggtgatcacg cgcctgttcg gtgtacagta ccatccctgt catgtttgga
     6541 aactcctgca tagcatggac tggagttgcc agaaaccgga gcgccgcgcg ttgcaacggg
     6601 acgaagcagc gatcacgcat tggaagcggc accgctggcc gcatataaaa aaacgccgca
     6661 aagtgtggcg cccatctcgt cttcctcgat gaatcggggt tcttgctcat tcccaacgtc
     6721 acccgcacct ggggaccccg cgggcacacg cctgtcgtct atcatctcta caaacatgac
     6781 cgcttgtcgg ccatcagtgc cctcgcggtc tcgccgcggc ggaggcgcat cgcgctgtat
     6841 ctccggctgc accggcaggc ccttgacggg ttggatgtcc gggcctttct cgcgtctctg
     6901 ctccggcacg tccgcggccc catcgtcctc ctgtgggatc agggcactat ccatcgccgc
     6961 cgtgaggtca cactctttct ccggcagcat ccgcgagtgc gggtggaaga gtttccaagc
     7021 tacgcccccg aactcaaccc ggcggagttt gtgtggacgc aaggcgaccg cagcttggcc
     7081 aacagcgccc cagccactgt ggcggacctg cgccagatgc tccgcaggtc gtaccgacgg
     7141 attcgggcgt cgcaacagtt gctgtggtcc tgcatcttcg cctctgatct cccgtggata
     7201 cgctaaacaa agccccagtc tgtcctcgta tgctacggat gaagcggcac ggcttgttgg
     7261 agtctcacgc cagtacctct cgaaaatggc tcacgagcag aggattcccg cgcgccggtc
     7321 aggctgcgat tggttattct accggagcgg gctcgtcagg ttcttcggcg ctcggcgggt
     7381 agggaaggcg gagtctccct cacggagagg ggaaggccga acgactgtga gcactgccga
     7441 ctgggagaaa cgagtgctcg cccagtcagg tgcgaaggag cccgataccg tgccaaggct
     7501 cgcccaggac cactcgtcgg gcagcctgtt cgcgcgtcga caagaggcag cctgacaaca
     7561 ccgcgatcgc gcacggcccg gcgtccagcc gagtagaaaa cgagcaccag gcagtggttc
     7621 tcagaaactg acaatcgtcc cgactgccgg ttggacgaag gccgaaggca tggcgcgcgc
     7681 caacgcgtga accgccttcc acccggtgca gtgagccggt agcacacgcc ctacctctgc
     7741 atcgataaat gctttcactg ttggatcgat gatcgcttcg aagatccccc cggtgagatg
     7801 gaagcctccg atgaatccgg caatccgctt ttccccggtg agttgctgtg cattgcgcag
     7861 cacgttcaca gcaccagcat gagagcaacc agacacgatg acgaggccct tgcctcgcac
     7921 attcacgatt acattctggt cgtcccagat catggggtcg ggcgtccagc cggcctgagt
     7981 ccgtgcatag tggattggga agcctttctc gaagtcggtc gtccgctcaa cctgaccgga
     8041 aatgagcacc gtgtcatcga gaagaagggt ctgccccctc tcttcgatca gttggagacc
     8101 ttcctcttcg aggtcgtgcc tgctcggcgg cggcaagcgt agttcggttc ccgtagggaa
     8161 gatgattttc cgctcacgcc aagcctcggg atggatgacg agcggca
//
LOCUS       DASQGH010000382         4098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55390, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000382 DASQGH010000000
VERSION     DASQGH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55390"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VEW91_07500"
     CDS             complement(<1..1102)
                     /locus_tag="VEW91_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91457.1"
                     /translation="MAVPPLSPGVAQGGPSAGDTLVFARQEETETLDPHKTTAISSAE
                     VDYLLYDTLTTLDYDNTVKPGLAEKWAISPDGKTYTFTLRPGVRFHSGKPLTATDVKF
                     TLERWKTIKGSPTAFNIAPVESVDAPNPRTVVLHLKEPLSILLVNLANYSGSILNPEV
                     VTRLGDDYGTGAGKVDGTGPFVLREWVRNDRLVSVRNPAYAWGPPIYKNPGPAHLASV
                     IFRTIVEDSPRVAAVEVGEAQFTPSVPPPQVARLAKDGRVKVIRYGDLNTVFLGFKLG
                     KKPLDDLKVRRAINYGINKPELIAGVYYGLADEAYGPLAPGTPGWWKDVTKVAYRYNP
                     STAKRLLDEAGWVQTRQGVPREKGGQPLSLLFL"
     gene            1324..1614
                     /locus_tag="VEW91_07505"
     CDS             1324..1614
                     /locus_tag="VEW91_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HYM91458.1"
                     /translation="MPTYVTLLKYTQKGVEDIKHGPDRLAAAKQRAKSKGGEIKAFYL
                     VMGRYDAVVISEAPNDEAAAAASLGAASQGFVSTETLRAFTEEEYRKLIASL"
     gene            1737..2714
                     /locus_tag="VEW91_07510"
     CDS             1737..2714
                     /locus_tag="VEW91_07510"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HYM91459.1"
                     /translation="MNAPEPVIAQLAHVELLTPRLEDSVWFFRDVLGLEESAREGGSV
                     YLRAYEDFYHHTLKLTEATGPGLGHVAWRAASEEALRALVQRLTAAGCGRGWSDGDLG
                     HGPAFRFITPEGHPMEVLWEVEYYAAPPEKRSLLRNRPQKRPLRGIPVRRLDHVNLMA
                     RSVAGMREFFERTLAFRLREQKIGEGGTEVGAWLSVSPLVHEIAIMRDATGVPGRFHH
                     LTYWYGYPQHLIDAADVCAENGITIEAGPGKHGTTQGMFLYVFEPGGTRVELFGDSGY
                     LIFDPEWKTVVWDVANESDLEKSSIWFGGRLPETFYTYGTPSREEAGRR"
     gene            complement(2734..3075)
                     /locus_tag="VEW91_07515"
     CDS             complement(2734..3075)
                     /locus_tag="VEW91_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HYM91460.1"
                     /translation="MTGDPRERARLRYIRQSIARIEQYTHGGREAFLSEPIVQDAVLR
                     RLETLADAIGKLSDSLKARHPTIPWRQVYGFRNVAAHAYEEIDLTRVWEIVEDYVPVL
                     KSVVDRELGEH"
     gene            complement(3072..3638)
                     /locus_tag="VEW91_07520"
     CDS             complement(3072..3638)
                     /locus_tag="VEW91_07520"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYM91461.1"
                     /translation="MAARATPVSALRVRKGPGSAEPFRRALSAARRAAGLTQAALAAR
                     LGTTQSAIARLERGEVAPTITTLSRLADALGIQFEVLPRSGIVVRETSPGLTLADLRA
                     RRDDLLAIAARHGARHVRVFGSVARGDARPDSDVDFLVEFAPGRTVLDLSGLILDLEE
                     TLGRKVNVVEVRRPSPAAERIRHEAVPL"
     gene            3776..4006
                     /locus_tag="VEW91_07525"
     CDS             3776..4006
                     /locus_tag="VEW91_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91462.1"
                     /translation="MASTEARGHATGAPPLHPVLEGWAGELATLNRSLSTIRVYLNDL
                     QQLLEWLGRKDSLTIEAADMRRYAHTMIKAGL"
BASE COUNT          664 a         1311 c         1491 g          632 t
ORIGIN      
        1 acagaaagag gagcgacagc ggctggccgc ccttctcgcg cggcaccccc tgccgggtct
       61 gcacccaccc cgcctcgtcg aggagccgct tcgccgtgct ggggttgtag cggtaggcga
      121 ccttggtgac gtccttccac caccccggcg tgccgggggc gagcggcccg taggcctcgt
      181 cggccaggcc gtagtagacg ccggcgatca gttcgggctt gttgatgccg tagttgatcg
      241 cccggcgcac cttgaggtcg tcgagcggct tcttgcccag cttgaagccg aggaacaccg
      301 tgttgagatc gccgtaccgg atgaccttga cccgcccatc cttggccagg cgcgcgacct
      361 ggggcggggg gaccgacggc gtgaactgcg cctcccccac ctcgacggcg gccacgcgcg
      421 gggagtcctc gacgatcgtg cggaagatca cgctcgcgag atgcgcgggg cccgggttct
      481 tgtagatcgg cgggccccag gcgtaggccg gattccgcac gctgaccagg cggtcgttgc
      541 gcacccactc ccggagcacg aacggcccgg tcccgtcgac cttcccggcc ccggtgccgt
      601 agtcgtcccc cagccgggtg acgacctccg gattgaggat gctccccgag tagttggcga
      661 ggttgacgag gaggatcgag aggggctcct tgaggtgcag gaccaccgtc cgggggttgg
      721 gcgcatcgac cgactcgacc ggggcgatgt tgaacgcggt cggcgatccc ttgatcgtct
      781 tccagcgctc gagcgtgaac ttgacgtcgg tggcggtcag gggcttgccc gagtggaacc
      841 ggacccccgg ccggagcgtg aacgtgtagg tcttgccgtc gggcgagatc gcccacttct
      901 ccgccagccc gggcttgacc gtgttgtcgt agtcgagcgt ggtcagggtg tcgtagagga
      961 ggtagtcgac ctcggcggag gaaatcgcgg tggtcttgtg cgggtccagg gtctcggtct
     1021 cctcctggcg ggcaaagacg agcgtgtccc cggcgctggg cccgccctgg gcgacgcccg
     1081 gggagagcgg cgggacggcc agctacgcaa cagcgcagag ggcgacgagc caccggtggg
     1141 tgatccactt catgggcgcc tccagatagg cctcggggcc gggtggggag cagggagggg
     1201 tgttcgggcg ctcccgccgc ttctcctctg cgcgggcgag cgccaagggg tttcggcggg
     1261 caggcccgaa ggccagacgt gcgtgggggg atgcatgcca atcgaccggg gggagtgaag
     1321 agcatgccga cgtatgtgac gcttctcaag tatacgcaga agggggtcga ggacatcaag
     1381 catggtcccg accgcctcgc ggcggcaaaa cagcgggcca agtccaaagg cggcgagatc
     1441 aaggcgttct acctcgtcat gggccggtat gacgcggtgg tcatcagcga ggccccgaac
     1501 gacgaagcgg cggccgcggc gtcgctcggg gctgcctcgc aaggatttgt cagcaccgag
     1561 accctgcggg ccttcacgga agaggaatat cggaagctca ttgcgagcct gtaagcaggc
     1621 tcgcgtctgc ggcgcccccc cacagcgtcg accccctcgg ctgacgatcg atggggtgtc
     1681 gcgacggtca ggggccgcac ccgccgaggg gcatcgggcg agactggagg ggaggcatga
     1741 acgcgccgga accggtgatc gcgcagctcg cgcacgtgga gctcctcacc ccgcgtctgg
     1801 aggactcggt gtggttcttc cgggacgtgc tggggctcga ggagagcgcc cgggagggcg
     1861 ggagcgtcta cctgcgggcc tatgaggact tctatcacca cacgctcaag ctcaccgagg
     1921 cgaccgggcc cgggctcggg cacgtggcct ggcgcgcagc ctcggaggag gcgcttcggg
     1981 cgctcgtgca gcgcctgacc gccgcgggct gcgggcgggg ctggagcgac ggggacctcg
     2041 gccacgggcc cgcattccgc ttcatcaccc cagagggcca cccgatggag gtcctgtggg
     2101 aggtcgagta ctacgcggcc ccgccggaga agcggagttt actccgcaac cggccgcaga
     2161 agcggcccct gcgcggcatc ccggtgcggc ggctggacca cgtgaacctg atggcgcgga
     2221 gcgtggcggg gatgcgcgag ttcttcgagc gcacgctcgc cttccgcctg cgcgagcaga
     2281 agatcgggga gggtgggacg gaggtggggg cctggctgag cgtcagcccg ctggtccacg
     2341 agatcgcgat catgcgcgat gccacgggcg tgccgggccg gttccaccac ctgacctact
     2401 ggtacgggta cccgcagcac ctgatcgacg cggccgatgt ctgcgcggag aacgggatca
     2461 cgatcgaggc cggcccgggc aagcacggca cgacccaggg gatgttcctg tacgtgttcg
     2521 agcccggggg gacgcgggtg gagctgttcg gcgacagtgg ctacctgatc ttcgatcccg
     2581 agtggaagac cgtcgtctgg gatgtcgcca acgaatcgga cctggagaag agcagcatct
     2641 ggtttggggg ccggctcccg gagacgtttt atacctacgg cacgccgtcg cgggaggagg
     2701 cgggtcgacg atgagcctgc cgtccgcgcc gattcagtgt tcgccgagtt cccgatcgac
     2761 aaccgacttg agtactggta catagtcctc aacgatttcc cacacccgcg tgagatcgat
     2821 ctcctcgtac gcatgagcgg cgacgtttcg gaagccgtac acctgccgcc aggggatcgt
     2881 aggatgccgc gccttgaggc tgtcagagag cttaccgatg gcgtccgcca gcgtttcgag
     2941 gcggcgaagt acggcgtcct ggacgatcgg ctcgctgaga aacgcctcgc gacccccgtg
     3001 agtgtactgc tcgatgcgcg cgatgctttg gcgaatgtac cgcagccgcg cccgctcgcg
     3061 cgggtcgccg gtcacagggg gacggcttca tgccggattc gctcggcagc cggcgatggg
     3121 cgtcgcacct cgaccacatt gaccttccgg cccagcgttt cttcgagatc gaggatgaga
     3181 ccgctgaggt cgaggaccgt ccggcccggc gcgaactcga cgaggaagtc gacatcgcta
     3241 tcgggccgtg cgtccccgcg ggccacggac ccgaacacgc gaacatgacg tgctccgtgc
     3301 ctcgccgcaa tggccaggag gtcatcccgg cgggcgcgga gatcggcgag cgtcaaccca
     3361 ggcgacgtct cgcgaacgac gatcccggac cgggggagca cctcgaactg gatcccaagc
     3421 gcgtccgcga gccgggacag ggtcgtaatg gtcggggcga cctcgccgcg ctccaggcgg
     3481 gcgatcgccg actgggtcgt gccgagcctg gcggcgagcg cagcctgcgt caggccagcg
     3541 gcccggcggg ccgcggaaag cgcgcgacga aagggctctg cagagcctgg accctttcga
     3601 acccgcaggg ctgacaccgg ggtggcacga gcggccattc ggcctcgacg acgcttcatg
     3661 gctttctccg gtagagtata gcatacatgc tatactcatt ggcggtggga gtaatcccgg
     3721 cggcgcatcg aagtattccg cataaggccc gtttacagaa ttgggggatc cctcgatggc
     3781 ctcaaccgaa gcccgggggc acgcgaccgg cgctccaccc ctccatccgg tgctcgaggg
     3841 gtgggcgggc gaactggcca cgctcaaccg aagcctcagc acgatccggg tctacctgaa
     3901 cgaccttcag cagcttctcg agtggctcgg gcggaaagat tcgctaacga tcgaggccgc
     3961 ggacatgcgc cggtacgccc acacgatgat caaggcaggc ctgtagccct ccgtggacgc
     4021 cgtggcggga ggtgggtcga cgatgagcct acggtcgctc tcgacccgga agtgtcaagg
     4081 atcgcagaaa cgttgcgg
//
LOCUS       DASQGH010000383         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41262, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000383 DASQGH010000000
VERSION     DASQGH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41262"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VEW91_07530"
     CDS             complement(<1..474)
                     /locus_tag="VEW91_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91463.1"
                     /translation="MNAAPHAVVAAENITKTYPMPGGPVHALRGVTLAVERGTFLAIA
                     GPSGCGKSTLLYLLGGVDRPTSGSVVLLGARTDTLGDAALARIRLHHVGLVFQRFFLL
                     PTLSAEENVALPMMEARVAPRECRRRARALLDRVGLADRARHRPGQLSGGEMQRVA"
     gene            complement(471..1688)
                     /locus_tag="VEW91_07535"
     CDS             complement(471..1688)
                     /locus_tag="VEW91_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYM91464.1"
                     /translation="MQPIAFLTVRTLVRSPARTALVVLGLSVIGALLLDMTMLAGGLQ
                     TSLGRVLSRLGFAVRVVPRGTLPFSGDAQIADAEALAAAIAAQRGVAAALPVIGTDLY
                     VQKGDRRIPAFALGVPAAGSDVYTVLDGRDLPATDAGSRAPDGVPIVVNQNMARLDGI
                     GIGDRVVLSRAPGPTLQMFAAVETGRVVGIGDFYFDLPTQRSVALANPALRRLQGRRG
                     AGASLILVRMTEPARADALARWITTRDPRVDAFSIQQFLDRAGARLTYFNQFSLILGT
                     ISITVAFLLITAIMTLSVGERLGEIAMLRALGFTRSRIVGLVVAEGVALSLAALPGTL
                     VLGVAIGDYLDRILRSAPAVPEGLHFFTWTTAAAARTTALLIATGALAGAYPAAVAAR
                     TGIAATLHREIVS"
     gene            complement(1699..2574)
                     /locus_tag="VEW91_07540"
     CDS             complement(1699..2574)
                     /locus_tag="VEW91_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYM91465.1"
                     /translation="MSRNGRRALWVAAAVALLWGVAGWGAPPPGAPRGGGQAEIPSVS
                     LGELAARRLGVGPGGLIEIGLSPAGPWRPARVAQVYRPVRYPTEVGREEIGVRLHLPD
                     LQALVGRGDEVDSIVVRLRAPASAPRVVEQLNAAALGFRAYTSADLAAQSSSTFEVIT
                     RFHRAIGIVTVFASSVFLVAIVTLRGEAMQQQVGILRLIGVSPRTVAGTMLLVAAGVA
                     LLGSGIGIGLGFLLSLAINAYYQRLFDTALVFSRITPSLVGIAATLSVLLGTAAGAVA
                     AGRLLHRRPLDQVGR"
     gene            complement(2571..3071)
                     /locus_tag="VEW91_07545"
     CDS             complement(2571..3071)
                     /locus_tag="VEW91_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYM91466.1"
                     /translation="MKHLWAPWRIQYIKGPPPEGCIFCTLPREGRDREHGILHQGRRA
                     FVILNTFPYNSGHLMIVPHRHVADLAALEDDEILELMHLTTAAMEAIRATYNSEGFNI
                     GVNVGRAAGAGILDHVHIHVVPRWVGDTNFMPVLGEVKVLPEDLSVTLDRLSQALRTV
                     LAGRTA"
     gene            complement(3068..3889)
                     /gene="rnc"
                     /locus_tag="VEW91_07550"
     CDS             complement(3068..3889)
                     /gene="rnc"
                     /locus_tag="VEW91_07550"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HYM91467.1"
                     /translation="MDPSARAAPAGPPEAAAGGHGARDEREPERQPGGQALSAEREAL
                     LAELEGKLGVKFRDRSLLHLALVHGSAGAEARTRHGENYERLEFLGDSVLNLAISDHL
                     YRLFPTRLEGDLARLRAGLVSEGALARVARDIDLGRYVVLGRGEEKGGGRSRPALLAD
                     ALEAVLGAVYIDSGYGVAHYCTTRWFGEALSELEEPGEGDYKSQLQELVQQLERRLPR
                     YRITGQRGPEHERAFVAIVEVNGRILGEGHGRSKKEAEQAAARRALDHLRRTRAG"
     gene            complement(3892..>4396)
                     /locus_tag="VEW91_07555"
     CDS             complement(3892..>4396)
                     /locus_tag="VEW91_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HYM91468.1"
                     /translation="AERRGARIYAEMIGYGKSADAFHITQPDPEGDGAARAIRNALKD
                     AGLEPAEVGYINAHGTSTPYNDRLETLAIKRVWGAEARRVPVSSTKSMTSHLLGAAGG
                     VEAITCALAIGRGMLPPTINYEEPDPECDLDYIPNAPRPARIDVAMSNAFGFGGHNAI
                     LVLRRPT"
BASE COUNT          650 a         1613 c         1545 g          588 t
ORIGIN      
        1 cgcgacccgc tgcatctccc cgccgctcaa ttgccccggc cggtgccggg cccggtcggc
       61 cagcccgacg cgatcgagca aggcccgggc gcgccgccgg cactcgcggg gcgcgaccct
      121 cgcctccatc atcggcaggg ccacattctc ttctgcggac agcgtcggca ggagaaagaa
      181 ccgctggaag acgagcccca catggtgcag ccggatgcgg gcgagcgcgg cgtcgccgag
      241 cgtgtccgtg cgcgcgccga ggaggacgac gctcccggag gtcggccggt ccaccccgcc
      301 caggaggtag aggagcgtcg acttgccgca gccggacggg cccgcgatgg cgagaaacgt
      361 cccccgctcc accgcgagtg tgaccccgcg gagcgcgtgc acgggtccgc cgggcatcgg
      421 gtacgtcttc gtaatgttct ccgccgcgac gaccgcatgg ggggcggcgt tcacgacacg
      481 atctcccggt ggagggtcgc ggcgatcccc gttctcgcgg cgaccgccgc gggatacgcc
      541 cccgccagcg ccccggtcgc gatgaggagg gccgtcgtcc gcgccgcggc ggcggtcgtc
      601 cacgtgaaga agtgcaggcc ctccggcacg gcaggggcgg agcgtagaat tcgatccagg
      661 tagtccccga tggccacgcc gagcacgagg gtgcccggga gcgccgccag ggacagcgcc
      721 acgccttcgg cgacgacgag cccgacgatc ctgctccggg tgaaccccag cgctctcagc
      781 atcgcgatct cgccgagccg ctcccccacc gacagcgtca tgatcgccgt gatcaggaga
      841 aacgccaccg tgatgctgat cgtcccgagg atcagggaga actggttgaa gtaggtgagc
      901 cgggctcccg cgcggtccag gaactgctgg atgctgaacg cgtcgacccg gggatcgcgg
      961 gtggtgatcc accgcgccag cgcatccgcc cgggcgggct cggtcatccg cacgagaatg
     1021 agcgaggcgc ccgcccccct ccgtccctgg agccgccgga gggccgggtt cgccagggcc
     1081 accgaccgct gggtgggcag gtcgaagtag aagtcgccga tgcccacgac ccggccggtc
     1141 tcgaccgccg caaacatctg cagggtcggc cccggcgctc gggagaggac gacgcggtct
     1201 ccgattccga tcccgtccag gcgcgccatg ttctggttga cgacgatggg gaccccgtct
     1261 ggggcccggg acccggcgtc ggtcgccggg agatcgcgcc cgtcgagcac ggtgtacaca
     1321 tctgatcccg cggcaggcac gccgagggcg aacgcgggga tccgccgatc ccccttttgc
     1381 acgtacaggt cggtcccgat gacgggaagg gcggcggcga ccccgcgctg ggcggcgatc
     1441 gccgcggcca gggcctcggc gtcggcgatc tgcgcatccc cggagaacgg gagagtgccg
     1501 cgggggacga cgcggacggc gaacccgagc cggctcagta cgcggccgag gctggtctgc
     1561 agccctcccg ccagcatcgt catatccagg agcaaggcgc cgatgacgct caggcccagc
     1621 acgaccaggg ccgtgcgcgc cggggagcgc acgagggtgc ggacggtgag gaacgcgatc
     1681 ggctgcacgc cgtcaggatc agcggccgac ttgatccagc ggcctccggt ggagcagccg
     1741 ccctgccgcg accgcgcccg cggccgtccc gaggagcacg gacagggtcg ccgctatgcc
     1801 cacgagggac ggcgtgatcc gcgagaagac cagggcggta tcgaagagcc gctggtagta
     1861 ggcgttgatc gcgagcgaga gcagaaatcc gagcccgatg ccgatcccgc tcccgagcag
     1921 ggccaccccg gcggccacca gcagcatggt acccgccacc gtgcgcgggg agaccccgat
     1981 gaggcgcagg atccccacct gctgctgcat cgcctcgccc cggagggtca cgatcgcgac
     2041 gaggaacacg ctgctggcga acacggtgac gatcccgatc gcgcggtgga acctggtgat
     2101 gacctcaaac gtgctcgaac tctgggccgc gaggtcggcg gaggtgtagg cgcgaaagcc
     2161 gagggccgcc gcgttcaact gctcgacgac cctgggggcg gatgccgggg cgcggagccg
     2221 caccacgatg ctgtccactt catcgccccg gcccacgagc gcctgcaggt cgggcaggtg
     2281 caggcggacc ccgatctcct cgcgccctac ctcggtcggg taccggaccg gccggtagac
     2341 ctgggccacc cgcgcaggcc gccacgggcc ggccgggctc agcccgatct cgatgaggcc
     2401 gcccgggccc accccgaggc ggcgcgcagc gagttcaccg agggacaccg acgggatctc
     2461 cgcctgcccc cctccccggg gcgccccggg cgggggcgcc ccccacccgg ccacccccca
     2521 cagcagcgcc acggcggcgg cgacccacag cgcgcgccgc ccgtttctgc tcatgcggtg
     2581 cggccggcca gcaccgtgcg gagcgcctgc gagagccggt caagcgtgac cgacaggtcc
     2641 tcggggagca ccttgacctc cccgagcacc ggcatgaagt tcgtgtcgcc cacccaccgc
     2701 ggcaccacgt ggatgtgcac gtggtccagg atgccggccc cggcggcccg gccgacgttc
     2761 acgccgatgt tgaacccctc ggagttgtag gtcgcgcgga tcgcctccat cgccgccgtg
     2821 gtgaggtgca tgagctcgag gatctcgtcg tcctctagcg cggccagatc ggccacgtgg
     2881 cgatggggca cgatcatgag gtgcccggag ttgtagggga aggtgttgag gatgacaaac
     2941 gcccgccggc cctgatggag gatgccatgc tcgcggtcgc gcccttcccg ggggagggtg
     3001 cagaagatgc agccttcggg gggtggcccc ttgatgtact ggatcctcca cggggcccag
     3061 aggtgcttca tcccgcccgg gtccgccgga ggtggtcgag cgcgcgtcgc gccgccgcct
     3121 gttcggcttc tttcttgctt cggccatggc cctcgccaag gatccggccg tttacctcga
     3181 cgatcgccac aaacgcccgc tcgtgctcgg gcccgcgctg cccggtgatc cggtaccggg
     3241 ggagccggcg ctccagctgc tggacgagtt cctggagctg gctcttgtaa tccccctccc
     3301 ccggctcttc cagctcgctc agggcctccc cgaaccaccg ggtggtgcag tagtgcgcga
     3361 ccccataccc cgaatcgatg tacaccgcgc ccaacaccgc ttccaacgcg tcggcgagca
     3421 gcgcgggacg gctccggcct ccgcccttct cctccccgcg cccgagcacc acataccgtc
     3481 ccaggtcgat gtcccgggcc acccgggcga gcgctccctc gctgaccaat ccggcccgca
     3541 gccgggccag atccccctcg agccgcgtgg gaaacaggcg gtagaggtga tcgctgatgg
     3601 ccaggttgag caccgagtcg ccgagaaact ccagccgctc gtagttctcg ccgtggcggg
     3661 tccgggcttc cgccccggca gacccgtgca cgagcgcaag atgcagcagc gaccgatcgc
     3721 gaaacttgac gccgagtttc ccctccagct ctgcgaggag cgcctcccgc tcggcggaga
     3781 gcgcctggcc tccgggctgg cgctcgggct cgcgctcgtc tcgcgcgccg tgcccgccgg
     3841 cagccgcctc aggcggcccc gccggcgcag cccgggcgga cggatccaat gtcaggtcgg
     3901 tctccggagc acgaggatcg cgttgtggcc gccaaaccca aaggcgttgg acatcgccac
     3961 atcgatccgg gccggccgcg gggcgttggg gatatagtcg agatcgcact cgggatccgg
     4021 ctcttcgtag ttgatcgtcg gagggagcat cccccgcccg atggcgagcg cgcaggtgat
     4081 cgcctcgacc ccacccgcgg cgccaaggag atgcgaggtc atggacttgg tcgaactcac
     4141 gggcacgcga cgggcctccg ccccccagac ccgcttgatg gccagggtct ccaaccgatc
     4201 gttgtagggg gtgctcgtgc cgtgggcgtt gatgtacccg acctccgccg gctcgagccc
     4261 cgcgtccttg agcgcgttcc gtatcgcccg ggccgcccca tcgccttccg ggtcgggctg
     4321 ggtgatgtgg aacgcatcgg cgctcttccc gtagccgatc atctccgcgt agatccgggc
     4381 cccccggcgc tcggcg
//
LOCUS       DASQGH010000384         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44144, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000384 DASQGH010000000
VERSION     DASQGH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44144"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VEW91_07560"
     CDS             complement(<1..311)
                     /locus_tag="VEW91_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYM91469.1"
                     /translation="MASDPSGAVRRAWRGVIEEYRAYLPVTSRTPLVTLLEGATPLLR
                     APRLMRHLPGVDLYLKYEGQNPTGSFKDRGMTLAMSKALEEGSRAVVCASTGNTAAAA
                     AA"
     gene            complement(301..1602)
                     /locus_tag="VEW91_07565"
     CDS             complement(301..1602)
                     /locus_tag="VEW91_07565"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYM91470.1"
                     /translation="MPHMVTDELRIGLLGCGTVGSAVVRLLEANAEEIYRRTGTRLRV
                     ASIAAAHPSKPRDLRLAPGILTGDARAVVASEQVDVVVEVIGGIEPARTLLLEAIRCG
                     KSVVTANKQLMARHGPELFAEAARAGVDLRLEASVGGGVPVIQPLRESLAANRFQQIV
                     GILNGTTNYILTKMTEERWEFARALEDAQRLGFAEADPTADVEGDDAAAKLAILATIA
                     FGTAVHLDDVYREGIRRVSAQDIRFAEELGFVIKLLAITREHAGRVEAHVHPALLPRA
                     HPLASVGNELNAVFVRGDAVGEVMFVGRGAGGGPTASAVVADLIDVARNRAAGTHGRI
                     GMRTLRRDPLRPMEETSSSFYLLMQVADRPGVFARIASIFGEEGVSIASIVQKSRGET
                     ADIVMVTHTTLESHMRRVLARVGQLDVVRAVRNVIRVLDGE"
     gene            complement(1626..2534)
                     /locus_tag="VEW91_07570"
     CDS             complement(1626..2534)
                     /locus_tag="VEW91_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYM91471.1"
                     /translation="MTGRPLSGVIVPIPTLFDDRGRLDEEANARHIDWLITQGVHGVF
                     ALGTTGEFTSLSGDERRLVAALAVRAARGRVPVLIGCSSAWTDEAIAYARHAEEIGAN
                     GVVAVLPYYWLPPERSIHEHYRLLAEGTSLPVYIYNFPALTGRSISPALVARLAAEHR
                     NIAGLKDTLDSIQHIQETIALVKPAHPQFAVLAGMDYHLLNTLLLGGDGGVPGTANFA
                     PEPFVRLYADVVAGKLEAAAERSRSLAPFTRLFTLEAAPFVIVKEAMVACGRIARATT
                     RPPALPLSDEQRRQLRKDLEATGVAR"
     gene            complement(2531..2755)
                     /locus_tag="VEW91_07575"
     CDS             complement(2531..2755)
                     /locus_tag="VEW91_07575"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYM91472.1"
                     /translation="MTAVHTAATEVEALMLKGALEATGIPVMLRSHRIPGYEQAIPPG
                     WGDLLVPDDRAREARELVAAYLDAQVEDRP"
     gene            complement(2752..3642)
                     /gene="rsmI"
                     /locus_tag="VEW91_07580"
     CDS             complement(2752..3642)
                     /gene="rsmI"
                     /locus_tag="VEW91_07580"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYM91473.1"
                     /translation="MATARSGVLYLVATPIGNLEDITLRALRLLREVACVAAEDTRRT
                     RALLSHFKIPARVVSLHEHNERARAPELIARLLAGESVALVSDAGTPGLSDPGTLVVR
                     QAAEAGIPVVALPGPSAFVTALVASGLPTAPVTFFGFLPTAPSERQRVLEAHRMVPHT
                     IVFYEAPHRLLKTLAAIRDAWGDRRIAVARELTKLHEEVFRGTLSEAIQRFTARPPQG
                     EFTLVIAGAEARTAGGPRDGAGPGADLHAGEAAQSMLRAALKEGVAPFDAVRRTAEAT
                     GLRRNAVYRMWLALKQEAAR"
BASE COUNT          513 a         1330 c         1328 g          532 t
ORIGIN      
        1 gcggcggccg cggccgcggt gtttccggtg ctcgcacaga cgaccgcgcg gctcccttcc
       61 tccagcgcct tcgacatcgc gagggtcatc ccccgatctt tgaacgaccc ggtcgggttc
      121 tgcccctcgt acttcaggta gaggtcgacg ccgggcagat ggcgcatgag gcgcggggcg
      181 cgcaggaggg gggtcgcccc ctcgaggagc gtgaccaggg gcgttcggga agtcacggga
      241 aggtaggcgc ggtactcctc gatcaccccc cgccacgcgc gccggaccgc ccccgagggg
      301 tcactcgcca tcgagcaccc ggatcacgtt gcgcaccgcg cggacgacat cgagctgccc
      361 gacgcgggcc aggacgcgcc gcatgtggct ctccagcgtc gtgtgcgtga ccatcacgat
      421 atccgccgtc tcgccccggc ttttctgcac gatcgaggcg atgctgacgc cctcctcccc
      481 aaagatcgag gcgatgcggg cgaacacgcc ggggcgatcc gccacctgca tgaggaggta
      541 gaacgacgag gacgtctcct ccatggggcg gagcgggtcc cggcggaggg tgcgcatgcc
      601 gatccgcccg tgcgtgccgg cggcccggtt ccgcgccacg tcgatcaggt cggccacgac
      661 cgcgctcgcg gtcgggcctc ccccggcgcc gcggccgacg aacatgacct cgccgaccgc
      721 atcgccgcgg acgaagaccg cgttgagttc gttcccgacc gaagcgaggg ggtgggcgcg
      781 gggcaggagc gccgggtgga catgcgcctc gacccggccg gcgtgctccc tggtgatcgc
      841 cagcagcttg atcacaaacc ccagttcctc ggcgaaccgg atgtcctggg ccgagacccg
      901 ccggatgcct tcccggtaca catcgtccag gtggaccgcg gtcccgaacg cgatcgtcgc
      961 gaggatcgcg agcttcgccg ccgcgtcgtc gccctcgacg tcggccgtcg ggtcggcctc
     1021 ggcgaacccc agccgctgcg cgtcctcgag cgcccgggca aactcccacc gctcctcggt
     1081 catcttcgtg aggatgtagt tggtggtccc gttgaggatc ccgacgatct gctggaagcg
     1141 gttggccgcc agcgactcgc ggagcggctg aatgaccggc acgcccccgc ccacgctggc
     1201 ctccagccgg aggtccaccc ccgcccgggc ggcttcggca aacagctcgg ggccgtggcg
     1261 ggccatcagc tgcttgttgg cggtgacgac gctcttgccg catctgatgg cctcgaggag
     1321 gagggtgcgg gccggctcga tccccccgat cacctccacg accacgtcga cctgctcgct
     1381 ggcgacgacc gcgcgcgcgt ccccggtcag gatgcccggg gcgaggcgga gatcgcgggg
     1441 tttggagggg tgggccgccg cgatgctggc gacgcgcagc ctggtcccgg tcctccggta
     1501 gatctcctcg gcgttcgcct ccaggaggcg gacaaccgcg ctgccgacgg ttccgcaccc
     1561 gagcaagccg atccggagtt cgtcggtgac catgtgcggc atcgtagcag gccgccccac
     1621 cagcgtcaac gcgcgacccc ggtggcctcg aggtccttcc gcagttgccg gcgctgttcg
     1681 tcggagagcg ggagcgcggg cggccgggtc gttgcccgcg cgatgcgccc gcatgcgacc
     1741 atggcttcct tgacgatcac gaacggcgcg gcctccagcg taaacagccg ggtgaacggc
     1801 gccagcgacc gcgaccgctc cgccgcggcc tccagtttcc ccgccaccac gtcggcgtag
     1861 agacgcacga acggctccgg ggcgaagttt gccgttcccg gcacgccccc gtccccgccg
     1921 agcagcaggg tgttgagcag gtggtagtcc atcccggcga gcaccgcaaa ctgggggtgg
     1981 gccggcttga cgagcgcaat cgtttcctgg atgtgctgga tgctgtcgag ggtgtccttg
     2041 aggcccgcga tgttccggtg ctcggcggcc agccgcgcga cgagcgcggg ggagatgctg
     2101 cgtccggtca gggcggggaa gttatagatg tagacgggaa gcgacgtgcc ctcggccagg
     2161 agccggtagt gctcgtggat ggaccgctcg ggggggagcc agtagtacgg caggaccgcc
     2221 accaccccgt tggccccgat ctcctcggcg tgccgggcgt aggcgatcgc ctcgtcggtc
     2281 caggcgctgc tgcacccgat gaggacgggg acccgaccgc gggccgcgcg gacggcgagc
     2341 gcagcgacca ggcgccgttc gtcgcccgac agtgacgtga actccccggt cgtgcccagg
     2401 gcgaagaccc cgtgcacccc ctgcgtgatc agccagtcga tgtggcgcgc attggcctct
     2461 tcgtccagcc gcccccgatc gtcgaagagc gtggggatcg ggacgatgac gccggacagc
     2521 ggcctgccgg tcacggacga tcttccacct gcgcgtcgag atacgcggcg acgagctccc
     2581 gcgcctcccg cgcgcgatcg tcgggaacga gcaggtcccc ccatccgggc gggatcgcct
     2641 gctcgtagcc ggggatccgg tgggaccgga gcatgaccgg gatccccgtc gcttcgagcg
     2701 cccccttgag catcagcgcc tcgacttcgg tggcggcggt gtgcacggcc gtcatcgggc
     2761 tgcctcctgc ttcagcgcca gccacatccg gtacaccgcg ttgcgacgga gcccggtggc
     2821 ctcggccgtg cggcgcacgg cgtcgaaggg ggccacccct tctttgagcg cggcgcggag
     2881 catggattgc gccgcctccc ccgcgtgcag gtcggccccc ggaccggccc cgtcccgggg
     2941 gccgcccgcg gtgcgcgcct cggctccggc gatcacgagc gtgaactcgc cctgcggggg
     3001 gcgcgcggtg aagcgctgga tcgcctcgct gagcgtgccc cggaatacct cctcgtgcag
     3061 cttcgtcagt tcgcgcgcca cggcgatccg gcggtctccc cacgcatccc tgatcgccgc
     3121 gagggtcttg agcagccggt ggggtgcttc atagaagacg atcgtgtgcg ggaccatccg
     3181 gtgcgcttcg agcacgcgct ggcgctcgga cggggcggtc gggaggaagc cgaagaatgt
     3241 caccggggcc gtcggcaggc cggaggcgac cagcgccgtg acgaacgcgc tcggccccgg
     3301 cagcgccacg acggggatgc ctgcctccgc cgcctgccgc accaccaggg tccccgggtc
     3361 gctgagcccg ggagtccccg catcggagac aagggcgacc gactctcccg cgaggaggcg
     3421 ggcgatcagt tcgggcgccc gtgcccgctc gttgtgctcg tggaggctca ccacccgggc
     3481 cgggatcttg aagtgcgaga gtagcgcgcg cgtgcgccgg gtatcctcgg ccgccacaca
     3541 cgccacctcc cggaggagcc ggagcgcgcg tagggtgatg tcctccaggt tgccgatcgg
     3601 ggtggctacc agatacagaa cgccggagcg cgccgtcgcc acatcctcac tataccagag
     3661 ggcttaccgg tcgggaacat tgggcgccgg ccgccgttac cga
//
LOCUS       DASQGH010000385         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52627, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000385 DASQGH010000000
VERSION     DASQGH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52627"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..988
                     /gene="pstB"
                     /locus_tag="VEW91_07585"
     CDS             200..988
                     /gene="pstB"
                     /locus_tag="VEW91_07585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HYM91474.1"
                     /translation="MERAVPVQGATAERIQAVRVSASYGRTPAVRGMSLGIQANRITA
                     IIGPSGCGKSTFLRCLNRMHELVPGARVEGRVLLDGEDIYAKDVDPVMIRRRVGMVFQ
                     RPNPFPTMSIYDNVAAGLRLNGVHNRGVLDEAVARSLRQAVLWDEVKDVLGRSGVSLS
                     GGQQQRLCIARALAVGPEVLLMDEPASALDPASTLKIEELAKTLRESLTIVLVTHNMQ
                     QAARVSDSTAFMLSGELVEFASTADLFTRPRDQRTEDYITGRFG"
     gene            1146..1280
                     /locus_tag="VEW91_07590"
     CDS             1146..1280
                     /locus_tag="VEW91_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91475.1"
                     /translation="MHTDDARTPGARWLDDAKAVLTIACVLLAVAIAAAAGVKLVLGH
                     "
     gene            1361..2020
                     /gene="phoU"
                     /locus_tag="VEW91_07595"
     CDS             1361..2020
                     /gene="phoU"
                     /locus_tag="VEW91_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HYM91476.1"
                     /translation="MATRETFDRDLEQLKRDLLRMGDLVDTAIRQAIEALQARSAEQA
                     EAVVVSDNAIDALHLELEDRCLRLVATQQPMAKDLRMIAAIWTMTIDLERMGDHAEDI
                     ARITRRIAEEPLLKPLIDIPRMADLVREMLRDGLDAFVRRDAGLAEQMALKDDAVDHL
                     YAQVFRELLTYMIEDPRNIQRATHLLMVAQALERMGDHATNIAERVIYMVTGTLRELN
                     V"
     gene            2328..3158
                     /gene="proC"
                     /locus_tag="VEW91_07600"
     CDS             2328..3158
                     /gene="proC"
                     /locus_tag="VEW91_07600"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HYM91477.1"
                     /translation="MRDYAFGFIGAGNMAEALLRGIFEAGLLDPAQCFLTNKSNDERL
                     ARLSRAWDLRTTRDKARLLAESDIVILAVKPHDMPTVLEEIAPHVGPRHLIISVAAGV
                     STGTIEGRLGAVPVVRVMPNTSTAVRASATAVAQGRHVDPRHLAIVRQLFEAVGWVGV
                     VPEPLLDVVTAVSGSGPAYVYALAEALVESGERAGLPRDLALALVAQTVLGAGKMLTE
                     TGEGPTVLRDRVTSPGGTTKAGLEALEQGGLRAAVQAAVTRATHRARELRDAPARSRG
                     "
     gene            3155..3664
                     /locus_tag="VEW91_07605"
     CDS             3155..3664
                     /locus_tag="VEW91_07605"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYM91478.1"
                     /translation="MRPGEGQRTPGAMPVSDLEVRVRYAETDQMGVAHHASYLVWFEA
                     GRTEFIREQGRSYAQIEAGGWLLVVVEARCRYLRPARYDDVLIIRTHLASLGPASLEF
                     AYEAIRKADGAVLARGATVHAAVDRTGRPHRIPEDLRRLLGGRAAGGPPPKGRAALPG
                     ELEALDRAH"
BASE COUNT          568 a         1236 c         1351 g          546 t
ORIGIN      
        1 tcacggtgca gctctataac tatgcgatcg cgccgtacga cgactggcat cgccaggcgt
       61 gggccgcggc gctggtcctg atgtcgatca cgctgctcct gaatatcacc gcccggctcc
      121 tcggccggcc gcggtcggga acgagggcat gaggatggac atctccggca cggtgtggaa
      181 tcgcgccctg ttcggcgcca tggagcgcgc ggtgccggtc cagggagcca ccgccgagcg
      241 cattcaggcg gtgcgcgtga gcgcctccta tggtcggacg ccagcggtgc gcggcatgtc
      301 gcttggcatc caagccaacc gcatcaccgc gatcatcggt ccgtcggggt gcgggaagtc
      361 cacctttctc cggtgcctga accgcatgca cgagctggtc ccgggggccc gcgtggaggg
      421 ccgggtgctg ctggacggcg aggacatcta cgccaaagac gtggaccccg tgatgatccg
      481 ccggcgcgtg gggatggtct tccagcggcc caacccgttt cccaccatgt cgatctacga
      541 caacgtcgcg gccgggctcc gattgaacgg ggtccacaac cggggcgtcc ttgacgaggc
      601 ggtggcgcgg agcctgcggc aggcggtgct ctgggacgag gtcaaagacg tgctcggccg
      661 gtcgggcgtg agcctctccg gaggccagca gcagcggctc tgcatcgccc gggcgctggc
      721 ggtcgggccg gaggtgctcc tgatggatga gccggcctcg gccctcgatc ccgcgtccac
      781 gctcaagatc gaagagctgg cgaagacgct gcgggagagc ctgacgatcg ttctcgtgac
      841 ccacaatatg cagcaggccg cccgcgtctc ggacagcacg gcgttcatgc tcagcggcga
      901 actggtcgag tttgcgtcca ccgccgacct gtttacgcgg ccgcgggacc agcgcaccga
      961 ggactacatc acgggtcggt tcggatagga gccggcgggc ccccttgtga cgcttgcgcg
     1021 gacggtcggg tccccggctt cactcagcct tcaactatcc ttcaccccgg attaaccttc
     1081 gcccgctacg ctgagcagga tgggtcgggg cggcgagatg ggcggcctcc cccccgaggg
     1141 gcccgatgca caccgatgac gcgcgcacac ctggcgcgcg atggctcgac gacgccaagg
     1201 cggtcctcac gatcgcttgt gtcctgctgg ctgtcgcgat agcggccgcg gccggcgtca
     1261 agctggtgct gggccactga ggcgcggtcc gcgcgtcgtg agaggaggaa ggcgccccca
     1321 agaggaagcg cttctgcgag gcgtcccaaa gggggcgagg gtggccactc gggaaacctt
     1381 tgaccgcgac ctggagcagc tgaagcggga cctgctgcgg atgggcgacc tggtggatac
     1441 ggcgatccgc caggccatcg aggcgctcca ggcgcgcagc gccgagcagg cggaggcggt
     1501 ggtcgtctcc gacaacgcga tcgatgcgct gcatctcgag ctggaggatc gctgccttcg
     1561 cctggtggcg acccagcagc cgatggcgaa ggacctgcga atgatcgccg cgatctggac
     1621 gatgacgatc gacctcgagc gcatgggcga tcacgcggaa gacatcgcga gaatcacccg
     1681 gcggatcgcc gaggagcctc tgctgaagcc cttgatcgac atcccgcgga tggcggatct
     1741 cgtgcgggag atgctgcgcg acggccttga cgcctttgtc cgccgggacg ccgggctggc
     1801 cgagcagatg gcgctgaagg acgatgccgt cgaccacctc tacgcccagg tcttccgtga
     1861 gctcctcacc tatatgatcg aggatcccag gaacatccag cgggccaccc acctcctgat
     1921 ggtcgcccag gcgctggaac ggatggggga ccatgcgacg aacatcgccg agcgggtgat
     1981 ctacatggtg accgggacgc tccgggagtt gaacgtctag ggaggccatt gacgcggcca
     2041 atccggcggt gctatagtta gcggacgacg caagagccgt gacggggcac gtaggacgtc
     2101 tccctgcgcc gagagagcgg cggagtccgg tggaagggct gccgcaggga catccgaacc
     2161 caccccccga gctgctcccg gggcggcgat cgccgccgaa cgggagccgg gtcgcccgag
     2221 atagcgtgag tgctccggcc tctggccggg gaacgaaggt ggtaccgcgg aagcgcccct
     2281 ttcgcccttt ggcgaccggg gtgtttttta ttgcggaggg gtcggcgatg agggactatg
     2341 ccttcgggtt catcggcgcg ggaaacatgg ccgaagcgct gctccgcggc atcttcgagg
     2401 ccgggctcct ggaccccgcg cagtgcttcc tcaccaacaa gagcaacgac gagcggctgg
     2461 cccgcctgtc ccgggcgtgg gatctccgca cgacccggga caaggcccgg ctcctggccg
     2521 agagcgacat cgtcatcctg gccgtcaagc cgcacgacat gccgacggtg ctcgaggaga
     2581 tcgccccgca cgtcgggccc cgtcatctca tcatctcggt ggcggccggc gtctccaccg
     2641 gtacgatcga gggccggctg ggcgcggtgc cggtcgtccg ggtcatgccg aacacctcca
     2701 cggccgtgcg ggcgtccgcg accgcggtcg cgcagggcag gcacgtcgac ccacgtcatc
     2761 tcgcgatcgt ccggcaactc ttcgaagccg tcggctgggt gggcgtcgtc cccgaaccgc
     2821 tcctcgatgt cgtgacggcg gtctcgggca gcgggcccgc gtacgtgtac gccctggccg
     2881 aggcgctggt ggagagtggc gagcgggccg gtctgccgcg ggacctcgcg ctcgctctcg
     2941 tggcccagac cgtgttgggc gcgggcaaga tgctcaccga gaccggggag ggacccacgg
     3001 tgctgcggga ccgggtgacc tcccccggcg gcacgaccaa ggccgggctc gaggcgctcg
     3061 agcagggcgg attacgggcc gcggtccagg ccgcggtgac gcgggccacg caccgggccc
     3121 gggaactccg cgacgcgccg gcccggagcc gggggtgagg ccgggggagg ggcagcgcac
     3181 tcccggagca atgccggtct cggatcttga ggtccgggtc cgctacgcgg agaccgatca
     3241 gatgggcgtg gcccaccacg cctcgtacct ggtctggttc gaggccgggc ggaccgagtt
     3301 catccgggag cagggtcgct cctatgccca gatcgaggcc ggcggctggc tgctggtggt
     3361 cgtcgaggcc cggtgccgct atctccgccc cgcccggtac gacgacgtgc tgatcatccg
     3421 gacccacctc gcctcgctcg gccccgccag cctcgagttc gcctacgagg ccatccgcaa
     3481 ggcggatggg gcggtgctgg cccgcggggc cacggtgcac gcggcggtcg accggacagg
     3541 gcgtccccac cggatcccgg aagacctccg ccggctgctg gggggccggg cggccggcgg
     3601 cccgccgccc aaagggagag ccgcgttacc cggcgaacta gaggctctgg atcgggccca
     3661 ttgaaattga agaggaaggg tgagcggtcc atgcccgagg a
//
LOCUS       DASQGH010000386         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49782, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000386 DASQGH010000000
VERSION     DASQGH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49782"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VEW91_07610"
     CDS             complement(<1..741)
                     /locus_tag="VEW91_07610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM91479.1"
                     /translation="MNLKGSVAMVTGGTGGLGRRICRALVQRGVRVAVCYQRRQEEAA
                     AVAGELQALGGQAAPFPVDVGEAGSVDRLIAEVVRAFGRIDILINDAAYNKWIPFQRV
                     GDLTLDDWNRILTTNLTGPFLCIKAVAPVMKRQAQGRIVNISSIAGFGAAGSSIAYAV
                     SKSGLNHLTRCMAVALAPEILVNGVAPGYMEGTRMSENLAPEYRQRATQGALLQRATD
                     KDDVAELVVEFCRTDSITGQTLVVDAGRV"
     gene            complement(1150..>2128)
                     /locus_tag="VEW91_07615"
     CDS             complement(1150..>2128)
                     /locus_tag="VEW91_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91480.1"
                     /translation="LAFRWSGGTPRDPHAYDPSVDRATALMRRVLAGIKVVGTQKVVK
                     NLGGDLILRYDVPVIRLYVNDAGADPQQVADLALPWSTVPWHVLALLEEAAKRGIGAL
                     SPEAAASQRLRVLDLVRAAPQTERLGRLVDEFAARSYVPEALKGWASADEARRRWRAL
                     KDFYRASGHFLVTNGPYRLAKWSSAGVVLEAFRDLSYPLGVGSFDKDVFPRRASIPTL
                     SQGGARIEFRPEVERVIKYDRYYKIVREVLGSETTGAIDTVTPNCRYVIVGSDGRVVK
                     VGTAPDPKNGVYRIDLGDGLGPGAYTAFVAVLLSDNYLTPDVKVVSFRK"
BASE COUNT          365 a          763 c          663 g          337 t
ORIGIN      
        1 cacgcgcccc gcatcgacga cgagcgtctg cccggtgatg ctatcggtcc gacagaactc
       61 gacgaccagt tccgccacgt catccttgtc cgtcgcccgc tgcagcagcg ccccctgggt
      121 cgcccgctgg cggtactccg gggccaggtt ctcgctcatg cgcgtcccct ccatgtatcc
      181 cggcgcgacg ccgttcacca ggatctccgg ggccagggcg acggccatac atcgggtcag
      241 gtgattcaat cccgactttg agaccgcgta cgcaatcgac gatcccgcag caccgaatcc
      301 tgcgatcgag gagatgttga cgatccgccc ctgcgcctgg cgcttcatga ccggggccac
      361 ggccttgatg cacaggaacg ggccggtcag gttcgtcgtg aggatccgat tccaatcgtc
      421 gagggtcagg tcccccaccc gctggaacgg gatccacttg ttgtaggccg cgtcgttgat
      481 caggatatcg atgcggccaa acgcgcggac cacctcggcg atcaggcggt cgaccgagcc
      541 ggcctcaccg acatcgaccg gaaagggggc cgcctgtccc ccgagcgcct gcaactcccc
      601 ggcgacggcc gcggcctcct cctgccgtcg ctggtaacag accgcgacgc gaaccccgcg
      661 ctggaccagc gcccggcaga tgcggcgacc caaccccccg gtcccgccgg tcaccatcgc
      721 gaccgaccct ttgagattca tcgcccgcct ccggtccgta cggccggacc aaggaggcat
      781 acgaggggcc tgctggcgat ccctccgggg gttaccctcg agcgcgcggg aaatatcggg
      841 gctagcgccc cgcgatccac aacggcggga tgagcatccc ggtcatcagg gccagcagga
      901 agagcatcgc gtgcgtaaag agtctgagca cgtggcggtc caaactgtct cactccccag
      961 gcatcgtgtc ggttcgcttt catcctcgtc cagaaccctc tgcctgggca atcgatcgct
     1021 cgaaggattc ggggtcatcg agtgaattga tcggctgaag gagacgcggc ctcacaggaa
     1081 ctctgggact tggccatcgt ctcccgccat agggagtctt ctgaagccaa tctcgatagt
     1141 gtgtcaggcc tatttgcgga aggaaacgac cttgacgtcc ggggtcaggt agttgtcgct
     1201 gaggagcacg gccacgaaag ccgtatatgc gccggggccg aggccatccc cgagatcgat
     1261 gcggtacacg ccgttcttcg gatccggggc cgtccccact ttcaccacgc gcccgtcgct
     1321 ccccacgatg acgtaccggc agttgggcgt cacggtgtcg atggcccccg tcgtctcgct
     1381 tcccaggacc tcccggacga tcttgtagta ccggtcgtac ttgatgaccc gctccacctc
     1441 cggccggaac tcgatccgtg cgcccccctg cgacagcgtg gggatggacg cccgccgcgg
     1501 gaagacgtcc ttatcaaagg agccgacgcc cagcgggtag ctcagatcgc gaaaggcctc
     1561 cagcaccact ccggcggatg accacttcgc gagccggtag ggaccgttgg tcacgagaaa
     1621 atggccgctc gcccggtaaa agtccttgag cgcccgccac cgccgccggg cctcgtcggc
     1681 ggacgcccat cccttgaggg cctccggcac gtagctccgc gcagcgaact catcaacgag
     1741 ccgccccagc cgctcggtct ggggcgccgc acgcacgaga tccagcacgc ggagacgctg
     1801 cgacgccgcg gcctcgggag agagcgcccc gatgccgcgc ttcgccgctt cttccagcag
     1861 cgccaggaca tgccacggca ctgtgctcca gggcagcgcc agatcggcca cctgctgggg
     1921 atcggccccg gcatcattca cgtacaagcg gatgaccggg acgtcatacc ggagaatcag
     1981 gtccccgccg aggttcttca ccaccttctg cgtgccgacg accttgatcc cggccagcac
     2041 ccgccgcatg agcgccgtgg cccggtccac cgaaggatcg taggcgtgag ggtcccgggg
     2101 ggtcccgccg ctccaccgga aggcgagg
//
LOCUS       DASQGH010000387         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1627, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000387 DASQGH010000000
VERSION     DASQGH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VEW91_07620"
     CDS             complement(<1..340)
                     /locus_tag="VEW91_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HYM91481.1"
                     /translation="MRRVRRQADRLRAAVAAAHAGAPWMAERLRASGLSPEGVHGIES
                     LERLPVLRKEALPAAQGRALPFGGLLGAPVASLRRIYVSPGPIYDPEPHTRDYWGLAP
                     ALHAAGFRRGD"
     gene            complement(385..1233)
                     /locus_tag="VEW91_07625"
     CDS             complement(385..1233)
                     /locus_tag="VEW91_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91482.1"
                     /translation="MGLGASLAPAARPGPPPAGGEDLVVNNIEVVYHDVIQVLKGVSI
                     SVPAHRIVTLLGTNGAGKTTTLRAISGLLRTEDGKVRDGTITYAGTRTDGLPPEAIVR
                     RGIVQVLEGRRVFKHLTVEENLRVGAVAHPEAFHARRDLVYTYFSPLGRHAHRLAGYL
                     SGGEQQMIAIGRALIALARLLLLDEPSLGLSPFLVQEIFGIIRRLRDEQGITVLLVEQ
                     NAHRALAIADYGYVMEGGKIVMEGPAAELRDNPDVKELYLGLTASGARRDYRAFKYYR
                     RRKRWL"
     gene            complement(1233..>2112)
                     /locus_tag="VEW91_07630"
     CDS             complement(1233..>2112)
                     /locus_tag="VEW91_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91483.1"
                     /translation="VPGISGWGTGDSILMKPQIETYGVPYIPASFHAGLLEPPNDWIW
                     LIGPSYTDHMKALLDYAKRSYKGIGKPRVALAVHNSDFGRSPVGPAREYGPKIGVEIV
                     AVEEVPANAIDATSQLLNMKRYAPTHIIIQNVARPCAIVVRDARKLNINAQVLGIHYI
                     GDELLFDLAGEAADGVIASTFVSNWFENVPGMALVHNINQTYHPGIIVRPLHYTQGIV
                     HAMVWVEALKRAQTPDRAGVAKALSGLGMRTDGLTAPISYGANNRKGASAIKLLQADV
                     KTRQFKLFVDWYAPPA"
BASE COUNT          302 a          734 c          746 g          330 t
ORIGIN      
        1 tgtccccgcg gcggaatccc gcggcgtgga gggcgggcgc gaggccccag taatcccgcg
       61 tgtgcggctc ggggtcgtag atcgggccgg gggagacata gatccgccgg agggacgcga
      121 ccggagcgcc gagcagcccg ccaaacggca acgcgcgtcc ctgcgcggcc ggcagggcct
      181 ccttgcgcag gacgggcagg cgctcgagcg actcgatccc gtgcacgccc tcgggagaca
      241 gcccggacgc gcgcaggcgc tcggccatcc acggcgcgcc ggcatgggcg gcggccaccg
      301 cggcccggag gcggtcggcc tggcgccgga cccggcgcac ggccggctcg ccctcgcccg
      361 gctcgtacag cgaatcggcg cggtctacag ccagcgcttc cgcctccggt agtacttgaa
      421 cgcgcggtaa tcccgccgcg cgcctgatgc ggtgagcccc agatataact ccttgacgtc
      481 gggattgtcc cgcaactccg cggccgggcc ctccatcacg atcttgccgc cctccatcac
      541 gtagccgtag tccgcaatgg ccagcgcccg gtgggcgttc tgctccacca ggagcaccgt
      601 gatcccctgc tcgtctcgga gccggcggat gatcccgaag atctcctgca cgagaaacgg
      661 cgagaggccc agcgagggct cgtcgagcag caggaggcgg gcgagggcga tgagcgcccg
      721 gccgatcgcg atcatctgct gctccccgcc agagaggtat ccggcgaggc gatgggcgtg
      781 gcggccgagc ggggaaaagt aggtgtagac gaggtcccgg cgcgcgtgga acgcctcggg
      841 gtgcgccacc gcgcccaccc gaaggttctc ctcgacggtc aggtgcttga acacccgccg
      901 accctcgagc acctggacga tcccccgccg gacgatcgct tcgggcggaa gcccgtccgt
      961 gcgggtcccg gcgtacgtga tcgtcccatc ccggaccttc ccgtcctcgg tccggagcag
     1021 cccggagatc gcccggaggg tcgtcgtctt tccggcgccg ttcgtcccga ggagggtgac
     1081 gatccggtgc gcggggacgc tgatcgagac ccccttgagc acctggatca catcgtggta
     1141 gaccacctcg atgttgttga cgacgaggtc ctcccccccg gccgggggag ggccggggcg
     1201 ggcggccggt gccagggacg cgccgaggcc catcaggccg gcggcgcgta ccagtcgacg
     1261 aagagcttga actgccgcgt cttgacgtcg gcctgcaaca gcttgatcgc gctggccccc
     1321 ttccggttgt tggcgccgta gctgatcggc gcggtgagcc cgtcggtcct catgccgagc
     1381 ccgctgagcg ccttggcgac cccggcgcgg tcgggggtct gcgcccgctt gagcgcctcg
     1441 acccagacca tcgcgtggac gatcccctgg gtgtagtgga gcggccggac gatgatgccg
     1501 ggatggtagg tctggttgat gttgtgcacg agcgccatgc ccgggacgtt ctcaaaccag
     1561 ttgctcacga acgtgctggc gatcaccccg tccgcggcct cgccggccag gtcgaacaac
     1621 agctcgtcgc cgatgtagtg gatgccgagc acctgggcgt tgatgttgag cttccgcgcg
     1681 tcccggacga cgatcgcgca ggggcgcgcg acgttctgga tgatgatgtg ggtcggcgcg
     1741 taccgcttca tgttgaggag ctggctcgtc gcgtcgatgg cgttcgccgg gacctcctcc
     1801 acggccacga tctcgacccc gatcttcggc ccgtactccc gagcgggtcc caccggcgat
     1861 ctgccgaaat ccgagttgtg gacggccagg gccacgcgcg gttttccgat ccccttgtag
     1921 gaccgcttgg cgtaatccag cagcgccttc atgtggtcgg tgtagcttgg cccgatcagc
     1981 cagatccagt cgttcggcgg ctcgagcagc ccggcatgga agctcgcggg gatgtagggg
     2041 acgccgtagg tctcgatctg cggcttcatc aagatggagt cccccgtgcc ccagccgctg
     2101 atccccggca cc
//
LOCUS       DASQGH010000388         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44150, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000388 DASQGH010000000
VERSION     DASQGH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44150"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VEW91_07635"
     CDS             <1..300
                     /locus_tag="VEW91_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HYM91484.1"
                     /translation="VLVLMALYIAGFALVANAQEAGRPAGAGIGAGLLGVGAGIAIGF
                     GAVGTGIAQSRIGGAAAGVVAERPEMFGIMLVLLVIPETLVIFGFVIAFFLYGKI"
     gene            306..890
                     /locus_tag="VEW91_07640"
     CDS             306..890
                     /locus_tag="VEW91_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HYM91485.1"
                     /translation="MSSELLRLLEQEAQSERDKALGEARTRAEQIVASARQEAEAIRA
                     EHRRRAEGERTQIRTQAASAASLRAAALLLQAKDEAIQAVFARATDELRRVSEDPARR
                     RTLLRHLIEEAAEGLSANRAILEVAPGDAAPAKAVCRDLGLAVEVRETPEASGGIRLT
                     TEDRRITVENTVASRLSRTRSSLVSRVAEILWGQ"
     gene            921..>1563
                     /locus_tag="VEW91_07645"
     CDS             921..>1563
                     /locus_tag="VEW91_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HYM91486.1"
                     /translation="MGDFSYINARVKVMKSHLLPAGRVEELLGAQDLEALILALSDTP
                     YHMELQEALSRFRGARAVDEALARNFYHATRRILGFADGSPRRQIEVVLLRYDLQNIR
                     GIVRGRHTGKTEEEILATLYPGGVLSEAKLHELLQQPDLRAIADTLVTWMHPLGRALR
                     QGVDAAQRSESLLDIEMALDRAYAQYGLRIADGEGGGEAAFRRFLQAEITATNV"
BASE COUNT          234 a          524 c          576 g          229 t
ORIGIN      
        1 gtcctggttc tgatggccct gtacatcgca ggcttcgcgc tggtggcgaa cgcgcaggag
       61 gcggggaggc ctgcgggagc cggcatcggc gcggggttgc tgggggtcgg cgcgggcatc
      121 gcgatcggat tcggggcggt cggcacgggg atcgcccagt cccgcatcgg tggggcagcg
      181 gctggcgtcg tcgccgagcg ccccgagatg ttcggcatca tgctggtcct cctcgtcatc
      241 ccggaaacgc tcgtgatctt tgggttcgtg atcgcgttct tcctctacgg aaagatctga
      301 gcgcgatgag ttccgagtta ctccggctgc tcgagcagga agcccaatcc gagcgggaca
      361 aggcgctcgg cgaggcgcgc acccgcgccg agcagattgt cgcctccgcc cggcaggagg
      421 cggaggccat ccgtgccgag caccggcggc gggcggaggg cgagcgcacg cagatccgga
      481 cccaggccgc cagcgcggcg agccttcggg ccgccgcgct cctgctgcag gccaaggacg
      541 aggcgattca agcggtcttt gcgcgcgcca cggacgagct caggcgcgtg agcgaagatc
      601 cggcccggcg ccggacgctg ttgcgccacc tcatcgaaga agcggccgag gggctgtccg
      661 cgaatcgggc gatcctcgag gtggcgcctg gggacgcggc cccggcgaag gcggtgtgtc
      721 gggatctcgg cctggcggtg gaggtgaggg agacccccga ggcgagcggg ggcatccggc
      781 tgacgaccga ggaccggcgg atcaccgtcg aaaacacggt cgccagccgg ctgagccgga
      841 cccgctcgtc cctggtttcg cgcgtggcag agatcctgtg ggggcagtaa ccccggtggc
      901 ccctgggccg gcgccgcgcc atgggtgatt tttcgtacat caacgcccgc gtgaaggtga
      961 tgaagtcgca cctgctcccc gcgggccgcg tggaggagct cctcggagcg caagatctcg
     1021 aggcgctcat cctggccctc tccgataccc cgtaccatat ggagttgcag gaagccctca
     1081 gccgcttccg gggcgcgcgc gcggtcgacg aagccctggc ccggaacttc taccacgcca
     1141 cccggcggat tctcggcttc gcggatggct ctccccgccg ccagatcgag gtggtgctcc
     1201 tccgctatga tctccagaac atccgcggga tcgtgcgcgg ccggcacacc gggaagaccg
     1261 aggaggagat tctggcgacc ctctacccgg gcggggtgct gagcgaggcc aagctgcatg
     1321 aactgctcca gcaacccgat ctgcgggcga tcgccgacac cctcgtgacc tggatgcatc
     1381 ccctcgggcg ggccctccgc cagggcgtgg acgcggccca gcgaagcgag agcctgctcg
     1441 acatcgagat ggcgctcgac cgggcctatg cccagtacgg gctgcggatc gccgatggcg
     1501 aggggggcgg cgaggcggcc ttccggcgct tcctccaggc cgagatcacc gcgaccaacg
     1561 tca
//
LOCUS       DASQGH010000389         1806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13143, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000389 DASQGH010000000
VERSION     DASQGH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13143"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VEW91_07650"
     CDS             <1..450
                     /locus_tag="VEW91_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYM91487.1"
                     /translation="RAWLAELPDRLEDPAFLAVHGSPRDPIEEYIIDLPTSLAIFSEH
                     AFSLCLVGHSHIPGAFILAADGTVSARALPVGKGVRLTRASRYIVNVGSVGQPRDGDP
                     RASYLVLDTTARTVALQRLEYPVAVTQEKMVARGLPVPLAQRLALGR"
     gene            563..>1806
                     /locus_tag="VEW91_07655"
     CDS             563..>1806
                     /locus_tag="VEW91_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HYM91488.1"
                     /translation="MEDLRARDDGARRPITIEDLLALKIADDPQVSPDGSRVACTVTV
                     ADAAANAYQTHLWIVPVDHGAPWQLTTARSKDTTPRWSPDGTRIAFISDRTGEKQVWV
                     IGPDGGEARQLTSGTLSPSEVSWSPDGRWLAVVGKPQPPSGPADSDVRVFSRLRYKFD
                     GDGFWDGRWKQVLVLPADGGEARQVTHEECDHITPAWSPDGRWLAYAANPRPDADLTN
                     VTDIWIVPADGGTAPRCLTRGIGPAGQPAWSPDGGRIAYVGHDNACRGATNSAVWVVP
                     SQGGDPICLTRLLDRSVGHHVTTDMRAHPRAGGLGWAPDGSRVVFMYAEGGSTQIASV
                     TVASGAVRPETTGDHELVGCSLDRAGRRVACVESDPLSPGEVAVRDLGDGAGPLRRIS
                     DWNGPLLRTVGLSVPERFECPGA"
BASE COUNT          260 a          644 c          642 g          260 t
ORIGIN      
        1 cgggcgtggc tagccgagct ccccgaccga cttgaggatc ccgcgtttct cgccgtccac
       61 ggaagcccgc gcgatccgat cgaggagtac atcatcgatc tgcccacctc cctcgccatc
      121 ttctcggagc acgcattctc cctgtgcctc gtgggccact cccacattcc cggggcgttc
      181 atccttgccg ccgacgggac ggtgagcgcc cgggcgctgc cggtggggaa gggcgtccgg
      241 ctcacccggg cgtcccggta tatcgtcaac gtggggagcg tcggccagcc ccgggacgga
      301 gatccgcgcg cgtcctatct tgtgctcgat accaccgccc gcaccgtggc gctccagcgc
      361 ctcgagtatc cggttgcggt cacgcaggag aagatggtgg cccggggcct gcccgtgccg
      421 ctggcgcagc ggctggccct cggacgctag gattaagacc gcagggagca cgtcgcgcgc
      481 cggagaaggg gaagcgaaga cgctacaccc gcgccgttcc taccggcggc ccggcgccgc
      541 atgataccag agggagggaa gagtggaaga tcttcgtgcg cgggacgatg gagcccggcg
      601 gccgatcacg atcgaggatc tgcttgcgct caagatcgcg gacgaccccc aggtgtcccc
      661 cgatggatcg cgcgtggcat gcaccgtgac ggtcgcggac gcggcggcca acgcctacca
      721 gacccacctc tggatcgtcc cggtcgatca cggcgcgccg tggcagctca ccaccgcccg
      781 cagcaaggac accacccctc ggtggtcccc ggatggaacc cggatcgcgt ttatctcgga
      841 ccgaaccggg gagaaacagg tctgggtgat cggccccgac ggcggagaag cgcgccagct
      901 cacgtcgggc acgctgtcgc cgtccgaggt gtcctggtcc cccgacggtc ggtggctcgc
      961 cgtggtcggc aaaccccagc ccccctccgg gcccgccgac agcgacgttc gcgtcttctc
     1021 ccggctgcgc tacaagttcg atggcgacgg cttttgggac ggacgctgga agcaggtgct
     1081 ggtgcttccc gcggacggag gggaggcccg acaggtcacg cacgaggagt gtgaccacat
     1141 cacccccgcg tggtcgcccg atggccggtg gctcgcctat gccgcgaacc cccgccccga
     1201 cgccgacctg acgaacgtca cggatatctg gatcgtgccg gcggacggcg ggacggcacc
     1261 gcgctgcctg acccggggca tcggacccgc aggccaaccc gcgtggtccc ccgatggggg
     1321 ccggatcgcc tatgtcgggc acgataacgc ctgccgggga gcgaccaact ccgcggtgtg
     1381 ggtcgtgcct agccagggcg gggatcccat ctgcctgacc cgcctgctcg accggagcgt
     1441 gggccaccat gtcaccaccg atatgcgcgc ccacccccgc gcgggcgggc tcgggtgggc
     1501 gcccgacggg tcccgggtgg tcttcatgta cgccgagggg gggagcaccc agattgcctc
     1561 cgtgaccgtc gcgagcggcg cggtgcggcc ggagacaacc ggggaccacg agctggtcgg
     1621 atgttcgctc gatcgcgccg gccgccgggt ggcgtgcgtc gagagcgatc cgctgtcccc
     1681 cggagaggtc gccgtccggg atctgggtga cggggcgggt cccctccggc ggatctcaga
     1741 ctggaacggg ccgctcctcc ggacggtggg cctgtccgtc ccggagcggt tcgagtgccc
     1801 cggggc
//
LOCUS       DASQGH010000390         1927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7451, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000390 DASQGH010000000
VERSION     DASQGH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7451"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VEW91_07660"
     CDS             <1..577
                     /locus_tag="VEW91_07660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYM91489.1"
                     /translation="APRARGTPVVVDTDEHPRPDTTLEGLAALRPAFRAGGTVTAGNS
                     SGINDGAAALLVASAEWAEREGRRPIARIAAAAVAGVDPSYMGIGPVPASRKALARAG
                     RRIDEMELIELNEAFAVQVLACVQDLGIDRTRLNPNGGAIALGHPIGASGARILTTLV
                     HELRRRQARWGLATMCIGVGQGIATVVEGLA"
     gene            581..1474
                     /locus_tag="VEW91_07665"
     CDS             581..1474
                     /locus_tag="VEW91_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HYM91490.1"
                     /translation="MQARSLLFTLYGDYIRHYGGTIWVGSLIALLSELGFTPSAVRAA
                     VSRMTRQGWLVSIRSGRASYYALSGRGQERIDEAAQRIFKLHPERWDGRWRLLVVGGE
                     RDPKQREGLRRELAWMGFAALARGVYLSPNDLLDRTASLADRYGLADRFETFTAEHEG
                     PTPDARLAAGHWDLRAIDAAYEGFVAEWRPRLESHRAGLSRGEALPDPVCFVEKTRLV
                     HAFRKFLFIDPGLPQDLLPERWAGTEARGVFSAYYHLLTEGALRFFERHYRPAPGHEG
                     DLPRGRQAARRDPFRPPIPVV"
     gene            1526..>1927
                     /locus_tag="VEW91_07670"
     CDS             1526..>1927
                     /locus_tag="VEW91_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91491.1"
                     /translation="MMRLATVVFTLALTAGLLAPLSAESPTRGGTVQVVLDRDPPTMD
                     PHLSGSAVDRQVYQNLYDKLVDIDDQLAIVPMLATSWTISQDGRTVTFKLRGGVKFHD
                     GTPFTADAVKANFDRMRDPQFPSVRRSEIAPV"
BASE COUNT          272 a          671 c          707 g          277 t
ORIGIN      
        1 cgccccgcgg gcccgcggca ccccggtggt cgtggacacc gacgagcacc ccaggcccga
       61 caccacgctt gaggggctcg cggccctgcg cccggcgttc cgcgccgggg ggaccgtgac
      121 cgcggggaac tcctcgggga tcaacgacgg cgctgccgcg ctcctcgtgg cgtccgccga
      181 atgggcggag cgggaaggac ggcggccgat cgcccgcatc gccgcggccg cggtggccgg
      241 cgtggacccg tcgtacatgg gcatcgggcc ggttcccgcg agccggaagg ctctggcgcg
      301 cgccggacgg cgaatcgacg agatggagct gatcgagctg aacgaggcgt ttgccgtcca
      361 ggtgctggcg tgcgtgcagg acctgggcat cgaccggacc cggctcaacc ccaacggcgg
      421 ggcgatcgcg ctcgggcatc ccatcggcgc gagcggggcg cgcatcctca ccacgctggt
      481 ccacgaactg cgccgccgcc aggcgcgctg gggcctggcg acgatgtgca tcggggtcgg
      541 ccaggggatc gcgacggtcg tcgaggggct ggcgtagcgc atgcaggccc gatcgctgct
      601 gttcacgctc tacggggact acatccggca ctacggaggc acgatctggg tgggcagcct
      661 catcgcgctc ctgagcgagc tcgggttcac cccgtcggcc gtgcgtgcgg cggtgtcgcg
      721 aatgacccgg cagggctggc tcgtctcgat ccggtcgggt cgggcgagct actacgccct
      781 gtcgggccgc ggacaggaac ggatcgacga ggccgcccaa cggatcttca aactgcatcc
      841 ggagcggtgg gacggccggt ggcggctcct cgtcgtcggg ggggagcgcg accccaagca
      901 acgggagggg ctgcgacggg agctcgcgtg gatggggttc gccgccctcg cgcgcggcgt
      961 gtatctctcg cccaacgacc tcctcgaccg gaccgcctcg ctggcggacc ggtacgggct
     1021 ggcggaccgg tttgaaacgt tcaccgccga gcatgagggc ccgaccccgg atgcgcggct
     1081 ggcggccggg cactgggatc tgcgcgccat cgacgccgcc tacgaggggt tcgtggccga
     1141 atggcggccg cgcctcgaat cacaccgcgc cggcctctcc cgcggcgaag ccctccccga
     1201 cccggtgtgc ttcgtggaga agacccggct ggtgcacgcg ttcaggaaat tcctgttcat
     1261 cgatcccggg ctcccgcagg acctgctccc ggaacgctgg gccggaaccg aagcccgggg
     1321 cgtgttctcc gcctactacc acctgctgac cgaaggagcg ctgcgcttct tcgagcggca
     1381 ctaccggccc gcgccgggcc acgagggcga cctgccgcgg gggcgtcagg cggcccgccg
     1441 ggatccgttc cgcccgccca ttccggtcgt gtgatcgatg agcgacgccg gtgtcctcac
     1501 cgcgcgtgaa aggggaggag gaggcatgat gagactcgcg acggtggttt tcactctggc
     1561 gctgaccgcc gggctcctgg cgccgctatc ggccgagagc cccacccggg gcgggacggt
     1621 tcaggtcgtg ctcgatcgcg accctcccac gatggatccg cacctctcgg gctcggccgt
     1681 ggatcgccag gtctaccaga acctgtacga caaactcgtg gacatcgatg atcagctggc
     1741 catcgtgccg atgctggcca cgtcctggac gatcagccag gatgggagga ccgtgacgtt
     1801 caagctgcgc gggggggtga agtttcacga cgggaccccg ttcaccgcgg acgcggtgaa
     1861 ggccaacttc gatcgcatgc gggaccccca gtttccttcg gtccggcgca gcgagatcgc
     1921 cccggtc
//
LOCUS       DASQGH010000391         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44166, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000391 DASQGH010000000
VERSION     DASQGH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44166"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VEW91_07675"
     CDS             <1..256
                     /locus_tag="VEW91_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HYM91492.1"
                     /translation="YQLHVTRERTLDALEVRVESAPSYAADGDALGARGAETAALANR
                     VAERLRGLTGIGIRVSIVPPATLPRSEGGKLRRVIDERSR"
     gene            complement(287..1528)
                     /gene="fabF"
                     /locus_tag="VEW91_07680"
     CDS             complement(287..1528)
                     /gene="fabF"
                     /locus_tag="VEW91_07680"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYM91493.1"
                     /translation="MRRRVVVTGLGAVTPIGHGSRGLYEGLRRERSAIDRLTRFDSEP
                     FNCKIAGEVRDFDPEAYLEPKRLRRLDRYSQFAVACARMASEDARLALAEENRDAIGC
                     FVGSALGGAAFAEDQSRVFMTQGIHRVRPTLALSVFAGAASCNVAIELDLRGPSSANS
                     DSCSSGAIAIGEAFRTVRDGYADLMLAGGVEVPLTPLIFGAFAVIRAMSTRNDEPARA
                     CRPFDRGRDGFVMAEGAALLVLEELEHARRRGAPIYGEVLGYGTSNDAHHMTAPLPSG
                     LQAARAMRCALEEAGVPPEAVDYVNAHASSTPLNDAIETLAIKQVLGEHAYRVPISGT
                     KGMHGHALGATGAIEAAICMLALRHAYLPPTVNLEDPDPACDLDYIPGRGREVAVRHI
                     LSNSFGFGGINASLVFGGASD"
     gene            complement(1525..2016)
                     /locus_tag="VEW91_07685"
     CDS             complement(1525..2016)
                     /locus_tag="VEW91_07685"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYM91494.1"
                     /translation="MHTETVACINGSADAVFAYAARVEDWPRLLPHYRSVRVIASRGG
                     ERLVEMRARRDRIPVWWVARQIVVAAERRIRYTHVRGVTRGMEVEWRITPAEEGVVVT
                     IVHDLSLRWPLIGRAVADRIIGPKFVEPIARATLRRIKWLVEQGTAAPAPIPAQMSGG
                     GKS"
     gene            complement(2017..>3228)
                     /locus_tag="VEW91_07690"
     CDS             complement(2017..>3228)
                     /locus_tag="VEW91_07690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYM91495.1"
                     /translation="DAMLDVIIVGAGPAGSAAAAVLARRGCRVLTLERATFPRPKPCG
                     DYLNPGCGAVLARIGALEAVRHAAAPVSGMRIVAPDGTGATTAFSSGMGYALPRATLD
                     HLLAGHAAGLGASVVEEACVVGIAHEGGHIRVTAERGRGGRPLEHYHARIVIGADGLR
                     SRVARMTGTGGPPRAGRFTIGGYLGELAPAATGGGAPPPGEVHLDRDRYCGVAYLPGD
                     LANVTIALGRPELRTWRGALESRYWASLRAFPGLAGRLGRARLVGGLRAAGPLAYWRR
                     RAVADGVVLVGDAAAYMDPMTGQGVYLALRGAELAAEAVAGALDGGGPTARILAGYER
                     ERRRAFAEAFLLSHLLQYLAFRPSTAAHALRRMAQRPELGTRFIDAVGNVEHAASLFR
                     PGFVARLLGNA"
BASE COUNT          454 a         1221 c         1124 g          429 t
ORIGIN      
        1 ctaccagctg cacgtgacgc gcgagcgcac cctggacgcg ctggaggtgc gggtcgagtc
       61 cgccccgtcc tacgcggcgg acggggacgc gctgggcgcg cgcggggcgg agaccgccgc
      121 gctcgccaat cgggtcgccg agcgcctgcg gggcctgacg gggatcggca tccgggtcag
      181 catcgtgccg cccgccacgc tgccccggag cgaggggggc aaactgcgcc gggtgatcga
      241 cgaacggtcc cgctgagggc tggggagcgc cgcgagcccc ggcggttcaa tctgacgctc
      301 cgccgaatac gagcgacgcg ttgatccccc cgaacccgaa cgagttgctg aggatgtgcc
      361 gaaccgcgac ctcgcggccg cgcccgggga tgtagtcgag gtcgcacgcc ggatcaggat
      421 cctccaggtt cacggtcggc ggcaggtagg cgtgccggag tgccagcatg cagatcgccg
      481 cttcgatcgc ccccgtcgcg ccgagcgcat ggccgtgcat gcccttggtg ccgctgatcg
      541 ggacgcggta tgcgtgctct cccaggacct gcttgatcgc caatgtctcg atcgcatcgt
      601 tgagcggggt gctgctcgcg tgcgcgttca catagtcgac cgcctcgggc ggcaccccgg
      661 cctcctcgag ggcgcaccgc atcgcgcgcg cggcctgaag gcccgagggg agcggggccg
      721 tcatgtggtg cgcatcgttc gaggtgccgt accctaggac ttcgccgtag atcggcgccc
      781 cgcgccggcg cgcatgctcg agctcctcta gcacgaggag ggccgctcct tcggccatga
      841 cgaatccgtc gcgcccccgg tcgaacggcc ggcaggcgcg ggcgggttcg tcgtttctgg
      901 tggacatcgc ccggatgacc gcgaacgcgc cgaagatcag cggcgtcagc ggcacctcga
      961 cccccccggc gagcatgagg tcggcgtagc cgtcccgaac ggtccggaac gcttccccga
     1021 tcgcgatggc gccgctgctg cagctgtcgg agttcgcgct gctcggtccc cggaggtcga
     1081 gctcgatcgc cacgttgcag gaggccgcgc cggcgaagac cgaaagcgcg agggtcggcc
     1141 tcacgcggtg gatcccctgg gtcatgaaca ccctggactg gtcctccgca aatgccgcgc
     1201 cgcccagcgc cgagcccacg aagcacccga tcgcgtcccg gttctcctcc gcgagcgcga
     1261 ggcgggcgtc ctcgctggcc atccgcgcac acgcgacggc aaactgcgag taccggtcga
     1321 gccgccggag gcgcttcggc tccagatacg cttcggggtc gaagtcccgc acctcccccg
     1381 cgatcttgca gttgaacggc tccgaatcga accgcgtgag gcgatcgatc gcggaacgct
     1441 cacggcgcag gccctcgtac agtccccggc tgccgtgacc gatcggcgtc acggcaccca
     1501 gcccggtgac gaccacccgg cgtctcacga tttcccccct ccgctcatct gggcggggat
     1561 gggagcgggc gcggcggtgc cctgctccac gagccacttg atcctccgga gggtcgctcg
     1621 cgcgatcggt tccacgaact tcggcccgat gatccggtcg gccaccgccc ggccgatgag
     1681 cggccacctg agtgacagat cgtgcacgat cgtgaccaca acgccttcct ccgccggcgt
     1741 aatgcgccac tcgacctcca tgcccctggt cactccacgc acgtgcgtgt accggatgcg
     1801 ccgctcggcc gccacgacga tctggcgcgc cacccaccac acggggatcc ggtcgcgccg
     1861 ggcccgcatc tccacgagac gctcgccccc cctggaggcg atcacccgca cgctgcggta
     1921 gtgcgggagt agccggggcc agtcctcgac ccgggccgcg tatgcgaaca ccgcgtccgc
     1981 gctgccgttg atgcacgcca ccgtctcggt gtgcacttat gcgtttccca gcaggcgggc
     2041 cacgaatccc gggcggaaca gggaggcggc atgttcgacg ttgcccaccg catcgatgaa
     2101 ccgcgtcccc aactcgggcc gttgggccat ccggcgcaac gcgtgggctg cggtggaggg
     2161 ccggaacgcc aggtactgca gcaggtgcga gaggaggaag gcctccgcga acgcccgccg
     2221 ccgctcgcgt tcatagccgg cgaggatccg cgcggtgggg ccgccgccgt cgagcgcccc
     2281 cgcgaccgcc tccgccgcga gttccgcgcc acgcaacgcg agatacaccc cctgaccggt
     2341 catcggatcc atgtacgccg ccgcgtcgcc gacgagcacc accccgtcgg ccaccgcccg
     2401 gcggcgccag tatgcgagcg ggccggcggc ccggagcccg ccgacgagcc gggcgcgccc
     2461 caagcgaccc gccaagcccg ggaacgcgcg gagcgaggcc cagtaccggg attcgagcgc
     2521 tccccgccac gtgcgtagct cgggccgccc gagcgcgatc gtgacgttgg cgagatcccc
     2581 ggggagatac gctacgccgc agtaccggtc gcgatccagg tggacctcgc ccggcggagg
     2641 cgcacctcca cccgtggccg cgggcgcaag ctcgcccaga tagccgccga tcgtgaaccg
     2701 tcccgctcgg ggtgggcccc cggtgccggt catccgggcc accctcgacc ggaggccgtc
     2761 tgcgccgatc acgatgcggg cgtggtagtg ctccagcggc ctgcccccgc gcccgcgctc
     2821 ggcggtgacc cggatgtggc cgccttcgtg cgcgatcccc accacgcagg cttcttcgac
     2881 caccgaggcg cccaaccccg ccgcatggcc ggccagcagg tggtcgaggg tcgcccgggg
     2941 cagcgcatag cccatgccgc tggagaatgc ggtcgtggcc cctgtgccgt cgggcgccac
     3001 gatgcgcatc cccgagaccg gggcggctgc gtgcctgacc gcttccaggg cgccgatgcg
     3061 ggccaggacg gcgccgcacc ccgggttcag gtaatccccg cacggcttgg ggcgcgggaa
     3121 tgtggcccgc tccagcgtga gcacgcggca tccccgccgc gccaggaccg cggccgcggc
     3181 gctcccggcc ggaccggctc ccacgatgat cacatcgagc atggcgtc
//
LOCUS       DASQGH010000392         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47032, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000392 DASQGH010000000
VERSION     DASQGH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47032"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VEW91_07695"
     CDS             complement(<1..248)
                     /locus_tag="VEW91_07695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HYM91496.1"
                     /translation="MLTTLLLDAVRSAVILGGVLTAFAYVTLLERRLLARFQLRIGPN
                     RVGPLGLLQPLADGIKLIFKESFIPSRADRFVYVIAPI"
     gene            complement(248..>2494)
                     /locus_tag="VEW91_07700"
     CDS             complement(248..>2494)
                     /locus_tag="VEW91_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYM91497.1"
                     /translation="TQDAGEDMVVRTQSPRVREGQQGILEFLLVNHPLDCPICDKGGE
                     CPLQDNTLRFGPGQSRFIETKRTFRKHIPMGPVLVLDRERCILCWRCVRFGEILAGDD
                     ALKGFERGYHSQISTPALAPVESKFIGNTMEICPVGALTSATYRFRSRPWDNRRVPSV
                     CPHCGCGCATELNIRGSEVTRTRPREHPELNDIWLCDKGFFGYEFAASPERLTTPRVR
                     RDGALGEATWEEALDRVGAALRSAPADGIGVMGGARTTNEENYLLLHLFRGVLGTNHI
                     DFRTGTAHPQPTTTAPWGLDVAIADVERAGAILLVGCDLTEEYPIIWLRVKKAVDRGA
                     RLIIVHPWELEIGRWASHVFVHRCGTEAAVLGALAGQIPHEAAARAAGVSAQALGAAA
                     AALSGAPRRLVLVGQAALERPDGGAVLDAVDRLRAAHGGADLGVLRGRGNSGGAQLLG
                     LLPDMLPGYRPLTDAGARAALEAAWGRPVPTPPGRTVRGMLEAARAERLRVLYVVGAD
                     PATDYPDAGAWAEARQRVGTLIVHELFMTRTAASADVVLPVLSFAEKAGTVCNVEGRV
                     QRQDQAVLGPGMARSDALIFSQVASQLGAVLGYASWHDVFAEIGRVIPGWQEGARLVP
                     PRAPQTAGAGPLAPPAAPGAAGALALLTGSRLFDRGTMAVRCPGIRHQAGEPFVALHP
                     DDAGRLGLADGAPCEVRSPRGGLRLAARIWPGLRPGQVFIPRGYDAAPVNSLLDERGP
                     VAVAVRAG"
BASE COUNT          348 a          927 c          902 g          317 t
ORIGIN      
        1 atgatcgggg cgatgacgta cacgaaccgg tcggcgcggg atgggatgaa gctctccttg
       61 aagatcagtt tgatgccgtc ggccaggggc tgcagcaatc cgagcggccc cacgcggttg
      121 gggccgatcc ggagctggaa ccgggcgagg aggcggcgct cgagcagcgt cacgtaggcg
      181 aacgccgtca gcaccccacc gaggatgacg gcactccgca ccgcgtccag gagcagcgtc
      241 gtgagcatca gccggcccgg accgccacgg ccaccggtcc ccgctcgtcg agcaggctgt
      301 tcaccggggc cgcgtcatac ccgcgaggga tgaagacctg gcccgggcgc agccccggcc
      361 agatccgggc ggcgaggcgg aggcccccgc ggggggaccg cacctcgcag ggagccccgt
      421 cggccagccc gagccggccc gcgtcatccg ggtggagcgc cacaaacggt tccccggcct
      481 gatggcggat cccggggcac cggaccgcca tcgtcccgcg gtcgaacagc cggctcccgg
      541 tcaggagggc gagcgcccct gcggcgcctg gggccgccgg gggcgccagc ggacccgccc
      601 ccgccgtctg cggggcccgt ggtgggacga gccgggcgcc ttcctgccag cccgggatga
      661 cgcggccgat ctccgcgaag acgtcgtgcc aggaggcata ccccaggacc gcgcccaact
      721 gcgaggcgac ctgcgagaag atcagggcgt ccgagcgcgc catcccggga ccgagcaccg
      781 cctggtcctg ccgctggacg cgtccctcga cgttgcacac cgtgccggct ttctcggcga
      841 agctcaacac ggggaggacc acgtcggcgg atgccgccgt ccgggtcatg aacaactcgt
      901 ggacgatgag ggtgcccact cgctgccggg cctccgccca cgccccggca tcggggtaat
      961 cggtcgcggg atccgcgccg accacgtaga ggacccggag gcgttccgcg cgcgccgctt
     1021 ccagcatccc ccggaccgtt cgaccggggg gggtgggaac gggccgcccc caggcggcct
     1081 cgagcgccgc ccgcgcgccg gcgtcggtga gcgggcggta cccggggagc atgtcgggaa
     1141 ggagcccgag cagctgggcg cccccgctgt tcccgcggcc gcgcagcacc ccgaggtcgg
     1201 ctcccccgtg cgcggcccgc agccggtcca ccgcgtccag caccgcgccc ccgtcggggc
     1261 gctcgagcgc ggcctgcccc accagcacca accggcgcgg tgcgccgctc agggccgcgg
     1321 cggccgctcc gagcgcctgg gcggagactc ccgccgcccg ggcggccgcc tcatggggga
     1381 tctgtcctgc gagcgccccc aagacggccg cttcggtccc gcaccggtgc acgaacacat
     1441 ggctggccca ccgcccgatc tcgagttccc acgggtgcac gatgatgagg cgcgcgccgc
     1501 ggtcgacggc cttcttgacc cgcagccaga tgatcgggta ctcctcggtg agatcgcacc
     1561 ccaccagcag gatcgcgccg gcccgctcga cgtcggcgat cgccacatcg agcccccacg
     1621 gggcggtggt cgtcggctgc gggtgcgcgg tcccggtgcg gaagtcgatg tggttggtcc
     1681 cgaggacccc gcggaagaga tgcagcagca ggtagttctc ttcgttggtg gtccgggccc
     1741 cacccatcac cccgatgccg tcggccgggg ccgatcggag cgcggccccc acccggtcga
     1801 gcgcctcctc ccaggtggcc tcccctagcg cgccgtcgcg gcggacgcgg ggggtggtca
     1861 accgctcggg gctggcggcg aactcgtaac caaaaaaccc cttgtcgcac agccagatgt
     1921 cgttgagctc ggggtgttcc cgcggacgcg tgcgggtcac ctcgctcccg cggatgttga
     1981 gctcggtcgc gcacccgcag ccacagtgcg ggcagacgct ggggacgcgc cgattgtccc
     2041 acggccggga ccggaaccgg taggtggcgc tcgtgagcgc gccgaccggg cagatctcca
     2101 tcgtgttgcc gatgaacttg ctctcgaccg gggcgagcgc cggggtgctg atctggctgt
     2161 ggtacccccg ctcgaagccc ttcagggcgt cgtcgcccgc caggatctcg ccgaaccgga
     2221 cgcaccgcca gcagaggatg caccgctcgc gatcgaggac gagcaccgga cccatcggga
     2281 tgtgcttgcg aaaggtccgc ttcgtctcga tgaaccggct ctggcccggc ccgaaccgga
     2341 gcgtgttgtc ctggagcggg cactcgcccc ccttgtcgca gatgggacag tcgaggggat
     2401 ggttcaccag gaggaactcg aggatgccct gctgcccctc ccggacccgc gggctctggg
     2461 tgcgcaccac catgtcctcg ccggcgtcct gggt
//
LOCUS       DASQGH010000393         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55414, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000393 DASQGH010000000
VERSION     DASQGH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55414"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="tsaB"
                     /locus_tag="VEW91_07705"
     CDS             complement(<1..562)
                     /gene="tsaB"
                     /locus_tag="VEW91_07705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYM91498.1"
                     /translation="MPILAIETATPIASCAVLDASGMLAETIIRAPMRHLEWLLPAAD
                     EMLGHLGLSPDAIQGLAVSSGPGGFTGLRIGIATAAAWGKARAVPVLGVGTLEALAHV
                     PGADGLILPVLDAHRGEVAGALYRVGGDGSAECLVPPLVGSPEAVAAEAAQHPGPVLL
                     VGDGLVRYGAAMLGALGARAPPRAPHH"
     gene            complement(544..1056)
                     /gene="tsaE"
                     /locus_tag="VEW91_07710"
     CDS             complement(544..1056)
                     /gene="tsaE"
                     /locus_tag="VEW91_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYM91499.1"
                     /translation="MRDLSGPSLAICTHTEDQTRRLGRALGAALRESARVDGPQAVVA
                     LAGPLGTGKTCLVQGLAQGLGVSGAVRSPTFVLIHEHRGPVPLYHVDLFRLEAADLES
                     LGLEEIIDSPGVTAIEWGERATGMLPREHLMIEFRFGPAPSDRCLRLIPQGERYERLV
                     AALRGCPSSR"
     gene            complement(1059..1832)
                     /locus_tag="VEW91_07715"
     CDS             complement(1059..1832)
                     /locus_tag="VEW91_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HYM91500.1"
                     /translation="MMTLIGPVIIIVGIVVFSLLSSIVKIAREYERGVIFRLGRLQRA
                     KGPGVILLIPIVDRMLKIDLRVVTLDVPRQEMMTKDNVPVTVDAVVYFRVVNPEDAVV
                     KVENYPKATYLVAQTTLRSTIGQHELDELLSQRERINQQLQQVIDVRTEPWGIKVTLV
                     EVRDVILPDTMKRAMAKQAEMERERRAKVINAEGEFQAAERLVQAAAKIVTQPVALQL
                     RYLQALTEVATEHNSTIIFPIPIDIITPFLKKAGEDRGG"
     gene            complement(1829..>3178)
                     /locus_tag="VEW91_07720"
     CDS             complement(1829..>3178)
                     /locus_tag="VEW91_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HYM91501.1"
                     /translation="EQHRMPALRWLKVLVPAVLFFWVAPAAPQTAPPKAPAEPALHVD
                     VIRLEGVIAPATARYVIRAIREAEQDGAAALLIEMDTPGGLLKSMEDITKAMLNTNMP
                     TVVYVYPPGSRAASAGVFITYAAGVAAMAPTTHLGAAHPVGVGGGGGQEDKTLMAKVT
                     NDAVAAIRGYAARHGRNADWAEKAVRESVSITETQALSLHVIDLVAATPRELLDAIDG
                     RAVVTASGTRRVHTRNARLVDIPMDVTERFLALLSDPNIGFILMTMAIYGIIFELSNP
                     GSVFPGVIGGLALILALASFAVLEVNVAGLLLIGFALILFIADIKVPSHGVLTAGGIA
                     SFVLGSLLLTERQAPFLRISLTLIFTVAALTAAFFAFAVGAGLRAQRQKIQTGREGLV
                     GAVGVARSQLSPDGTVFVQGEIWTAESVDGVIPAGGRVRVVRVDGLRLRVKKEEAPV"
BASE COUNT          477 a         1126 c         1103 g          472 t
ORIGIN      
        1 ggtggtgcgg cgcccggggg ggggcgcggg cgcccagcgc cccgagcatc gctgcgccgt
       61 accgcacgag cccgtccccc acgagcagca ccgggccggg gtgctgagcg gcctcggcgg
      121 ccacggcctc gggggacccc acgaggggcg gcaccagaca ctccgcgctc ccgtctcccc
      181 cgacgcgata gagcgccccg gcgacctcgc ctcgatgcgc atcgaggacg ggcaggatca
      241 acccgtccgc cccggggacg tgggccagcg cctcgagggt gccgaccccc aggacgggca
      301 ccgcgcgcgc cttcccccag gctgccgcgg tggcgatccc gatgcgcagt ccggtgaatc
      361 cccccggccc gctgctcacc gccaacccct ggatcgcgtc gggggacagg cccaagtgcc
      421 cgagcatctc atccgcagcc ggcaggagcc actcgaggtg gcgcatcggc gccctgatga
      481 tggtctcggc gagcatgccc gacgcatcga ggacggcgca gctggcgatc ggggtggcgg
      541 tttctatcgc gaggatgggc atccgcgaag cgcggccacc agccgctcgt accgctcccc
      601 ctgggggatc aggcgcaggc agcggtcgct gggggcgggt ccgaaccgga actcgatcat
      661 gagatgctca cgcggcagca tcccggtcgc ccgctcgccc cattcgatcg cggtgacccc
      721 agggctgtcg atgatctcct cgaggcccag gctctcgagg tccgccgcct ccagccggaa
      781 gagatcgacg tgatagaggg ggaccggtcc gcggtgctcg tggatgagca cgaacgtggg
      841 actccgcacg gccccggaga cccccagccc ctgggcgagg ccctggacca ggcaggtctt
      901 gcccgtcccg aggggaccgg cgagcgcgac gaccgcctgg gggccatcga cccgggcgct
      961 ctcccgcagc gccgcgccca gcgcccggcc gagccgacgg gtctggtctt cggtgtgcgt
     1021 acagatcgcg agggagggtc ccgacagatc ccggatggtt accccccgcg gtcctccccg
     1081 gcctttttga gaaacggggt gatgatgtcg atggggatgg ggaagatgat cgtcgagttg
     1141 tgctcggtgg ccacctcggt gagcgcctgg agatagcgca gctgcagcgc gaccggctgg
     1201 gtcacgatct ttgccgccgc ctgcaccagc cgctccgcgg cctgaaactc gccctcggcg
     1261 ttgatgacct tggcccgccg ctcccgctcc atctccgcct gcttggccat cgcccgcttc
     1321 atcgtgtcgg ggaggatcac atcccgcacc tcgacgaggg tgaccttgat cccccacggc
     1381 tcggtccgga catcgatgac ctgctggagc tgctggttga tccgctcacg ctggctgagg
     1441 agctcgtcga gctcgtgctg tccgatggtg ctccggagcg tggtctgcgc gacgaggtag
     1501 gtggccttgg ggtagttctc gaccttgacg accgcgtctt cggggttcac gacccggaag
     1561 tagaccaccg cgtcgacggt cacggggacg ttgtccttgg tcatcatctc ctgccggggc
     1621 acgtccaggg tgacgacgcg gagatcgatc ttgagcatgc gatcgacgat ggggatgagg
     1681 aggatcacgc ccgggccctt cgcccgctgc agccggccga gccggaatat caccccgcgc
     1741 tcgtactccc gggcgatctt cacgatcgag gacagcaacg agaagaccac gatacctacg
     1801 atgatgatca cgggaccgat cagggtcatc ataccggcgc ttcctccttc ttcacccgca
     1861 ggcgcagccc gtcgacccgc accacccgca cccggccgcc ggccgggatc acgccgtcga
     1921 cgctttccgc ggtccagatc tcgccctgga caaacaccgt cccgtcgggg ctcagctggc
     1981 tgcgcgccac cccgaccgct cccacgagcc cctcccggcc tgtctggatc ttctggcgct
     2041 gcgcccgcag ccccgcgccg accgcgaacg cgaagaaggc ggcggtgagg gcggccaccg
     2101 tgaagatcag cgtcaggctg atccgcagaa acggcgcctg gcgctcggtg aggagcagcg
     2161 atccgaggac gaacgacgcg atgccgccgg cggtgagcac gccgtggctg ggcaccttga
     2221 tgtcggcgat gaacaggatg agcgcgaatc cgatgaggag cagccccgcc acattgacct
     2281 ccaggaccgc gaaggaggcg agcgccagga tcagggccag ccccccgatc acccccggga
     2341 agaccgagcc cgggttgctc aactcgaaga tgatcccgta gatcgccatc gtcatcagga
     2401 tgaatccgat gttgggatcg ctgaggagcg ccaggaatcg ctcggtcacg tccatcggga
     2461 tgtcgaccaa ccgcgcattc cgcgtgtgca cgcggcgcgt gcccgacgcg gtgacgaccg
     2521 cccgcccgtc gatcgcgtcc aggagctccc ggggggtcgc cgccacgagg tcgatgacat
     2581 ggaggctcag cgcctgggtc tcggtgatcg agacgctctc gcggacggcc ttctccgccc
     2641 aatccgcgtt gcggccgtgc cgggccgcgt acccccggat cgcggccacc gcgtcgttgg
     2701 tgaccttggc catgagggtc ttgtcctctt gtcccccacc tccgcccacg cccacgggat
     2761 gcgccgctcc cagatgggtc gtgggggcca tcgcggcgac cccggccgcg taggtgatga
     2821 agacccccgc ggaggccgcg cgggatcccg gggggtacac gtacaccacc gttggcatgt
     2881 tcgtgttgag catcgccttg gtgatgtctt ccatcgattt gagcagcccg ccgggggtgt
     2941 ccatctcgat caggagggcg gccgcgccgt cctgctcggc ctcccggatc gcgcggatga
     3001 cgtaccgggc cgtggccggg gcgatgaccc cttcgagccg gatgacgtcg acgtgtaacg
     3061 cgggttcggc gggcgccttc ggaggagcgg tttgcggtgc ggcgggggcc acccagaaga
     3121 acaggacggc cggcacgagc accttgagcc accggagggc gggcatgcgg tgctgttc
//
LOCUS       DASQGH010000394         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41311, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000394 DASQGH010000000
VERSION     DASQGH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41311"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..>1534)
                     /locus_tag="VEW91_07725"
     CDS             complement(182..>1534)
                     /locus_tag="VEW91_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HYM91502.1"
                     /translation="TVSLLTLVAADLAVFAFALSVAVAVRVSILPGLSRAFSRPTYPL
                     AHYIGLWWIPLAYLSALAYAGLYTRRDPYWEEARRCLIGATAATLLIFAILSVAKIGD
                     NLSRPVMLVAWIALLGTLPLARGAVKEALFAVGLWRRPAVLVGDGALARSLRDAFRRH
                     RTLGYDVVDVIADPGAAPARAAMCGAREVILATPQLERAEFLRLVERLRGVVENILIV
                     PDLAEAPVLGVEVLGLMEDRALLLRVPNNLLKPWNLAIKRVFDLVVGAMLALAALPLI
                     GVLAVAIRLDSPGPVFHVEPRIGRRRLPFACYKLRTMLQDADRLLEQFLASHPEAASE
                     WERFRKLRSYDPRVTRLGRFLRRYSLDEIPQLINVVRGEMSLVGPRPYLLPEMAVLEG
                     DSMCDVLPGMTGLWQVSGKNALELRERARLDRWYVNNWSLWLDIIVLAKTLPVILRAE
                     "
BASE COUNT          278 a          540 c          513 g          203 t
ORIGIN      
        1 gcggcggaac gccgaagggg gttatgcagg cgcgacgtcg tgcgcctgcc agccccgccc
       61 caggccggac ctaccgggaa tgtccgtcct cctccacata tgccgtaaac ccggccttct
      121 tagacacccc tgcccgccgc acgatccggg cccggtcccg gcgcgtcttg cgaccttccg
      181 cctactcggc ccgcaggatc accgggagcg tctttgccag gacgataatg tcgagccaca
      241 acgaccagtt gttgacgtac catcggtcga gcctggcccg ctcgcgcagt tcgagcgcgt
      301 tcttgccgct gacctgccac aggcccgtca tccccggcag cacatcgcac atgctgtcgc
      361 cttccagcac cgccatctcc ggcagaagat acggtctcgg gccgaccagg ctcatctctc
      421 cgcggaccac gttgatgagc tgggggatct cgtccagcga gtaccgccgg aggaacctcc
      481 ccaggcgggt cacccgagga tcgtacgacc gcagcttccg aaagcgctcc cactcggatg
      541 cggcttcggg gtggctcgcc aagaactgct cgaggaggcg gtcggcatcc tggagcatgg
      601 tgcgcagctt atagcaggca aaggggaggc gcctccggcc gatccgcggc tcgacatgga
      661 acaccggccc gggagagtcg agcctgatcg ccacggccag gacgccgatg agcggcaacg
      721 cggccagggc gagcatggcc cccaccacga ggtcgaatac ccgcttgatc gcgaggttcc
      781 agggcttgag cagattgttg gggacacgga ggagcagcgc ccggtcttcc atcaggccga
      841 gcacctcgac cccgagcacc ggggcttcgg cgagatccgg cacgatcagg atgttctcca
      901 ccaccccccg caggcgctcg acaagtcgca ggaactcggc gcgctcgagt tggggggtcg
      961 ccaggatcac ctcgcgggcc ccacacatcg ccgcccgcgc cggcgccgcg cccgggtccg
     1021 cgatgacgtc gacgacatcg tatccgaggg tgcggtgccg gcggaaagcg tcgcgcaggc
     1081 tccgagcgag cgcgccgtcg ccgaccagga ccgcgggtct gcgccacaac cccaccgcaa
     1141 acagcgcttc tttgacggcg ccgcgggcga gcggcagggt cccgagcaac gcgatccacg
     1201 ccacgagcat caccggtcgc gagagattgt cgccgatctt ggcgaccgac aggatggcga
     1261 agatgagcag ggtcgccgcg gtggccccga tcaggcagcg gcgcgcctct tcccagtagg
     1321 gatcccggcg agtgtacagg cccgcgtagg ccaacgcact gaggtaggcc aggggaatcc
     1381 accacaatcc aatgtagtgg gcgaggggat aggtgggccg ggagaaggca cgcgacagcc
     1441 ccggcaggat cgagacgcgg accgcgacgg ccacactcag cgcgaatgcg aacacggcca
     1501 ggtcggcggc cacgagcgtc aacagcgaga ccgt
//
LOCUS       DASQGH010000395         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41325, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000395 DASQGH010000000
VERSION     DASQGH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41325"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VEW91_07730"
     CDS             <1..291
                     /locus_tag="VEW91_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HYM91503.1"
                     /translation="STNDQLRQTWLRTAVPLCESLGFRVPAHHDSTTDTYVLDYPLPV
                     DFDEEARRWLMDRPITWNDVLTRWKRRGPGNAMFVEMLQRGRRQMRQMLGLH"
     gene            306..641
                     /locus_tag="VEW91_07735"
     CDS             306..641
                     /locus_tag="VEW91_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HYM91504.1"
                     /translation="MPVPPAPTAPTPDGVLAALSGVIDPEWPVSVVDMGLVYGVRVEE
                     GIVTLTLTFTATACPCMEMIQEDIRNRLLRMPGVRDVRIVVSWDPPWTKDRLTARARE
                     AFRHWGVTV"
     gene            651..878
                     /locus_tag="VEW91_07740"
     CDS             651..878
                     /locus_tag="VEW91_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91505.1"
                     /translation="MQRIGDEPVYEVFARRVDGEPLRHVGSVAAPDDALARVYARSIY
                     DEESWIEMYVVPRRAIIPVESVRTEAVDAHA"
     gene            875..1648
                     /locus_tag="VEW91_07745"
     CDS             875..1648
                     /locus_tag="VEW91_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HYM91506.1"
                     /translation="MSTPLLDRPSALPQETVPPLVDLVVALADNKHALGLRYGEWCSS
                     GPTIEAGVAATAMAQDELGHARVLYGLLDELPGAPHRSEHEWAAEDARAVALLDRPFP
                     SWAHLIVANILLDQALTFVLETALESRYLPLRQRTRKLIEEEQFHAIHGRGWLLQLAA
                     EGSSIRAALEATIREAWADAVCWFGPDRGGALAPLVAAGVLREAGDAVRRRFLRRIGP
                     LLHEAGLASPLRADGDGWVAAQPLPWAGWDESCRRMRRG"
     gene            1641..1841
                     /locus_tag="VEW91_07750"
     CDS             1641..1841
                     /locus_tag="VEW91_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91507.1"
                     /translation="MGDHSPARPGDAASSKPAVVCPFCGSDDTELINLFGSTSLTSQY
                     YCRKCRSAFERVKWGTSPAAGP"
     gene            1838..2752
                     /locus_tag="VEW91_07755"
     CDS             1838..2752
                     /locus_tag="VEW91_07755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM91508.1"
                     /translation="MTKIMPLEEAVARYVRDGMTIAMGTGLESCIPFAAGHAIIRQGR
                     RDLMLVGPVSDILFDMLIGAGCVARVIAAWVGNVGAGTAYNFRRAVEDGIPRAVAVED
                     HSNLTVALALQAAAMGISYLPTRTALGTDIVRDHPRMREVACPFTGERLLAVGAVRPD
                     IAILHAQRCDRHGNAHLWGNLGVSLEAARAAEAVLLTCEEVVEDGVITSDPNRTLVPG
                     FLVRAVAVVPFGSHPSPTQGYANRDDRFYDEYHAETRTREGFVSWLERMVLGTGGHAG
                     YLDRLGEDRLAHLRPRTSYLAAPVNYGL"
     gene            2753..>2980
                     /locus_tag="VEW91_07760"
     CDS             2753..>2980
                     /locus_tag="VEW91_07760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM91509.1"
                     /translation="MRGDRVTRRQVMVTAAAREIRDGDVVFVGMRLPLLGFALARALH
                     APNAVGLFENGVIRDTPPVAPIITMGDPPNIV"
BASE COUNT          411 a         1014 c         1105 g          450 t
ORIGIN      
        1 agcacgaacg accagctccg ccagacctgg ctgcgcaccg ccgtgccgct gtgcgagagc
       61 ctggggttcc gggtgccggc ccaccacgac tccaccaccg acacgtacgt cttggactat
      121 ccgctgccgg tcgacttcga cgaagaggcg cgccggtggc tgatggaccg accgatcacg
      181 tggaacgacg tgctcacgcg gtggaagcgc cggggcccgg gcaacgcgat gttcgtcgag
      241 atgctccagc ggggccggcg gcagatgcgc cagatgctgg ggctgcacta gcgatgcgcg
      301 gagcgatgcc cgttcctccc gcccccaccg cgccgacccc cgacggggtg ctggcggccc
      361 tctccggcgt catcgacccg gagtggccgg tcagcgttgt ggatatgggg ctcgtctacg
      421 gggtcagggt ggaggaaggg atcgtgaccc tgacgctgac gttcacggcg acggcgtgcc
      481 cctgcatgga gatgatccag gaggacatcc ggaaccgcct gctgcgcatg ccgggcgttc
      541 gggacgtcag gatcgtcgtc tcctgggatc cgccgtggac caaggaccgc ctgaccgcgc
      601 gggcgcgcga ggcgttccgg cactggggcg tcacggtgtg agggagcgtc gtgcagcgga
      661 tcggtgacga accggtctac gaggtgttcg cccgtcgtgt cgacggggag ccgcttcgcc
      721 atgtgggcag cgtcgccgcg cccgatgatg cgctcgcccg ggtgtacgcg cgcagcatct
      781 acgacgagga gtcctggatc gagatgtacg tcgtgccccg gcgcgcgatc atcccggttg
      841 agtccgtccg caccgaggcc gtggacgcac acgcatgagc accccactgc tcgaccgtcc
      901 ctcggccctc ccgcaggaga ccgtgccacc gctggtggac cttgtggtcg cgctcgccga
      961 caacaagcac gccctcgggc tgcggtacgg ggagtggtgc agcagcgggc cgacgatcga
     1021 agccggcgta gcggcgaccg cgatggccca ggatgagctg ggacacgccc gcgtcctgta
     1081 cgggctgctg gacgagctgc cgggagcccc ccaccgctcg gagcatgagt gggccgcgga
     1141 ggacgcgcgc gcggtcgcgc tcctcgaccg gccgtttccg agttgggcgc acctgatcgt
     1201 cgccaacatc ctgctcgacc aggcgctgac cttcgtgctt gagaccgccc tggagtcgcg
     1261 gtatctgccg ctccgccagc gcacccgcaa gctgatcgag gaagagcagt ttcatgcgat
     1321 ccacggccgg gggtggctgc tccagctggc ggcggaaggg tcgagcatcc gcgccgcgct
     1381 cgaggcgacc atccgggagg cgtgggcgga cgcggtgtgc tggtttggcc ccgaccgggg
     1441 cggcgcgctc gcgccgctgg tggcggccgg ggtgctgcgg gaggccggcg acgcggtgcg
     1501 ccggcggttc ctgcggcgga tcggcccgct gctccacgaa gcggggctcg cttccccgct
     1561 gcgggcggac ggtgatggct gggtggcggc ccagccgttg ccctgggccg ggtgggacga
     1621 gtcctgccgg cggatgcggc gtgggtgacc attcgcccgc caggccgggg gatgcggcgt
     1681 cgtccaagcc cgccgtggtc tgcccgttct gtgggtcgga cgacaccgag ctcatcaacc
     1741 tgttcggctc cacgtccctg acgtcgcagt actattgccg caagtgccgg tcggcgtttg
     1801 agcgggtgaa gtgggggacg tccccggccg caggcccgtg accaagatca tgcccctcga
     1861 ggaagccgta gcccggtacg tgcgggacgg gatgaccatc gcgatgggga ccgggctgga
     1921 atcctgcatc ccgttcgccg cgggccacgc gatcatccgg cagggccggc gcgacctcat
     1981 gctcgtcggg ccggtctccg atatcctgtt tgacatgctc atcggcgccg gatgcgtcgc
     2041 cagagtgatc gcggcgtggg tgggcaacgt cggcgccggg accgcgtaca actttcgccg
     2101 cgccgtggag gacgggatcc caagggcggt ggccgtcgaa gaccactcca atctcaccgt
     2161 ggcgctcgcg ctccaggcgg cggcgatggg catctcgtac ctgccgacgc ggacggcgct
     2221 ggggacggat atcgtccggg accacccgcg gatgcgcgag gtcgcctgcc cgtttaccgg
     2281 ggaacggctg ctcgccgtcg gggctgtgag acccgacatc gcgatcctgc acgcgcagcg
     2341 ctgcgaccgc cacggcaacg cgcacctgtg gggcaacctg ggcgtgtcgc tcgaagcggc
     2401 gcgggcggcc gaggcggtgc tcctgacgtg cgaagaagtg gtcgaggacg gcgtcatcac
     2461 gagcgatccc aaccgcacgc tggtgccggg cttcctcgtg cgcgcggtcg cggtcgtgcc
     2521 gttcggatcc cacccgtcgc cgacccaggg ctacgccaac cgcgacgacc ggttctacga
     2581 cgagtaccac gccgagacac ggacgcggga ggggttcgtg tcctggctcg agcgaatggt
     2641 cctggggacc ggcgggcacg ccgggtatct cgaccggctg ggcgaggacc ggcttgcgca
     2701 cctgaggccg cgaacctctt atctcgctgc gcccgtgaac tacggcctct agatgcgcgg
     2761 cgatcgtgtg acccgccgcc aggtgatggt cacggccgcc gcccgggaga tccgggacgg
     2821 ggacgtggtg tttgtcggca tgcgcctccc gctgctcggg ttcgcgctgg cccgggcgct
     2881 gcacgcgccg aacgccgtgg gactgttcga gaacggcgtc atccgggaca cgccgcccgt
     2941 ggctcccatc atcaccatgg gcgatccgcc gaacatcgtc
//
LOCUS       DASQGH010000396         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55437, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000396 DASQGH010000000
VERSION     DASQGH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55437"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VEW91_07765"
     CDS             complement(<1..714)
                     /locus_tag="VEW91_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91510.1"
                     /translation="MSPVAVAVLAAAVRAGTPVLFATLGEIFAERAGVLNLGVEGMML
                     VGALAGFAATARTGNPWIGALVAAAVGGGLSLLHAFLSVTLRANQVASGRALTIFGTG
                     LSAFLGRGYVGVPAAGFHPVPLPWLAHLPVLGPVLFQQDPLIYLSYLTVPAAWYVLYR
                     TRWGLDIRAVGEHPEAADAMGVDVARVRYACVVVGGLLAGLGGAFLSTAYTSMWIENM
                     TAGRGWIAVALVIFATWDPL"
     gene            complement(711..1811)
                     /locus_tag="VEW91_07770"
     CDS             complement(711..1811)
                     /locus_tag="VEW91_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91511.1"
                     /translation="MISLERRESPSPWAPLVIPACSIAMALIAGAGVLWLAGVNPLAA
                     YAEVLREVFGSGFGMAETTVKAIPLILTGLAVLLPARMKLWNIGAEGQLQLGAIGATW
                     VALWTPLGHSPVGIPALLLGGMAAGAAWGLIPGALRAWLNVNETITTLLMNYVALLFV
                     DLLIYGPWRDPASRGFPLSATFPPSAHLPILIPGTRVHLGLALALLAVIAVAIGLART
                     RWGFEIRVIGDNPEAARYAGISLARHTLLVMGIAGALAGLAGVGEASAIAGRLQRGLS
                     PGYGYTAIIVAWLAKLNPLSVVVVAFLLGGLFLGGDALQISFGLPIAMVNMLQGLIFF
                     FVLAGEVLAAYRVVPGRGRARVGAPRGSGVVR"
     gene            complement(1808..3334)
                     /locus_tag="VEW91_07775"
     CDS             complement(1808..3334)
                     /locus_tag="VEW91_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91512.1"
                     /translation="MPEPPAVEMRGITKRFPGILANDAVDFDLAAGEIHALLGENGAG
                     KSTLMKVLYGLYQPDTGEIRLRGTPVRFRSPRDALGHGLGMVFQQFMLVPSLSVTENV
                     LLALPRAGLRLNRREASARVGALASRYGMAVDPAARVWQLSVGEQQRVEILRLLARNA
                     EILILDEPTSVLTPQEVRDLFTALHRLAGEGRAIVIITHKLAEVVAVADRVTVLRGGR
                     RVGSVRAGETTPQALARMMVGREAFGQARRDPVPPGPVVLAVEGLRAVGDRGTPALRG
                     VSLSVRAGEIVGVAGVAGNGQRELGEIVAGLRRATGGRIRINGVDHTHASAREIRASG
                     VGHVPEDRLGTGVMPNLSVADNLLLTAYRSPRFGRGLLLDQRALLDYARALMAEFRIR
                     AAGPMVPARTLSGGNLQRLILARELSGHPRLILAFHPTRGLDVGGTEAVHELLLSQRR
                     AGAAILLISEDLGEILQLCDRIAVLYRGELAGMGEAGRVTVEALGLMMAGVRPAGTAP
                     "
BASE COUNT          602 a         1328 c         1238 g          411 t
ORIGIN      
        1 gagcggatcc caggtggcga agatgacgag cgccaccgcg atccacccgc gtccggcggt
       61 catgttctcg atccacatgc tcgtgtacgc ggtcgagaga aaggcccccc cgagcccggc
      121 cagcaggccc cccaccacca cacacgcgta ccgcacgcgc gccacgtcga cccccatggc
      181 gtcggccgcc tcgggatgct cgccgacggc ccggatgtcg aggccccacc gggtccggta
      241 caggacgtac caggcggccg gcacggtgag gtaactcagg tagatcaagg gatcctgctg
      301 aaacagaacg ggccccagca cggggaggtg cgcgagccat ggcaggggaa ccgggtgaaa
      361 gcccgctgcc ggcacgccga cgtaccccct cccgagaaac gccgagagcc cggtcccgaa
      421 gatcgtgagc gcccgcccgc tcgcgacctg gttcgctcgc agcgtcacgc tgagaaaggc
      481 atgaagcagg ctgaggccgc cccccaccgc cgcggcgacc agggcgccga tccacggatt
      541 gcccgtgcgg gccgtggcgg caaaccccgc cagcgccccg accagcatca tcccctccac
      601 ccccaggttg agcaccccgg cccgctccgc gaagatctcg cccagcgtgg caaagagcac
      661 cggcgtcccg gcccgaacgg ccgcggcgag caccgccacc gccaccggac tcatcgcacc
      721 accccagacc cgcgcggcgc cccaacccgg gcccgccccc tcccggggac cacccggtac
      781 gccgcgagca cctcgccggc cagcacgaag aagaagatca atccctgcag catgttgacc
      841 atggcgatgg ggaggccgaa cgagatctgc agcgcgtccc cgccgagaaa gaggccgccc
      901 agcaggaacg ccacgacgac cacggacagc gggttgagct tggccagcca ggcgacgatg
      961 atcgccgtgt acccgtaccc gggcgacagc ccccgctgga ggcgccccgc gatcgccgac
     1021 gcctccccca cgcccgccag ccctgccaaa gccccggcga tccccatgac gagcagggtg
     1081 tggcgcgcaa ggctgatccc cgcgtaccgg gccgcctcgg ggttgtcgcc gatgacccgg
     1141 atctcaaacc cccaccgggt gcgggccagc ccgattgcga cggcgatcac ggccagcagc
     1201 gcgagcgcga gccccaggtg cacgcgcgtg cccgggatga ggatcgggag gtgcgccgag
     1261 ggggggaacg tcgccgagag ggggaacccg cggctggcgg ggtccctcca cgggccatag
     1321 atcaggaggt ccacaaacag gagggcgacg tagttcatca agagggtggt gatggtctcg
     1381 ttcacgttga gccaggcgcg cagcgctccg gggatgaggc cccacgcggc cccggcggcc
     1441 atcccaccca gcaggagggc ggggatcccc accggcgagt ggccgagcgg cgtccacaac
     1501 gcgacccagg tggcgccgat cgcgccgagc tgcagttgcc cctcggcccc gatgttccag
     1561 agcttcatcc tggccgggag cagcacggcc agcccggtga ggatgagggg gatggctttg
     1621 acggtcgtct ccgccatgcc gaacccgctg ccgaacacct cgcgcaggac ctccgcgtac
     1681 gctgccaggg gattgacccc cgcgagccac agcacacccg cccccgcgat gagggccatc
     1741 gcgatcgagc acgcggggat cacgagcggc gcccagggag acgggctctc ccggcgctcg
     1801 agcgagatca tggggcggtc ccggcggggc gcacgccggc catcatgagc ccaagggctt
     1861 ccacggtcac gcggccggcc tcacccatcc ccgcgagctc gccgcggtac agcacggcga
     1921 tccgatcgca gagctgcagg atctcgccga ggtcctcgga gatcagcagg attgcggcgc
     1981 cggcgcggcg ctggctcaac agcagctcgt gcaccgcctc ggtgccgccc acgtcgagcc
     2041 cccgggtcgg gtgaaatgcc aggatcagcc ggggatggcc cgacagttcc cgggccagga
     2101 tcaggcgctg caggttgccg cccgacagcg tccgcgcagg gaccatcggc ccggccgcgc
     2161 ggatcctgaa ctcggccatg agggctcgcg cataatccag cagcgcccgc tggtcgagga
     2221 ggaggccccg gccgaaccgc ggggagcgat aggcggtaag caggaggttg tcggccacgc
     2281 tgaggttggg catgacgccc gttcccaggc ggtcttcggg cacatggccg acgccgcttg
     2341 cgcggatctc acgggcggag gcgtgcgtgt ggtccacgcc gttgatgcgg atccgaccgc
     2401 cggtggcgcg gcgcaacccc gcgacgatct cccccaattc gcgctggccg ttcccggcga
     2461 ccccggccac cccgacgatc tcaccggccc ggaccgagag ggagacgccg cgcagggcgg
     2521 gcgtgccccg gtcgccgacc gcacggaggc cctcgacggc gagcaccacc gggcccggag
     2581 gaacgggatc gcgcctggcc tggccaaagg cttcccggcc caccatcatc cgcgccagcg
     2641 cctgcggggt cgtctccccg gcccggacgc tcccgacacg ccggccgccc cgcaggacgg
     2701 tgacgcggtc agccaccgcg acgacctccg ccagcttgtg cgtgatgatg acgatcgccc
     2761 ggccctctcc ggccagccgg tggagggcgg tgaagaggtc ccgcacctcc tgcggcgtca
     2821 ggaccgaggt cggctcatcg aggattagga tctctgcgtt gcgggcgagg agcctgagga
     2881 tctccacccg ctgctgctca cccacgctca gctgccacac gcgggcggcc ggatcgaccg
     2941 ccatcccgta gcgggaggcc agcgcgccca cacgggcgga cgcctcccgg cgattgaggc
     3001 gcaaccccgc gcgcggcagg gcgagcagca cgttttcggt gacggacagc gacgggacga
     3061 gcatgaactg ctgaaagacc atccccaatc catgcccgag ggcgtcgcgc ggggagcgga
     3121 accggaccgg ggtcccgcgg agccggatct ctcccgtgtc gggctggtag aggccgtaga
     3181 ggaccttcat cagcgtggat ttccccgccc cgttctcgcc gaggagcgcg tggatctccc
     3241 ccgcggcgag atcgaagtcc accgcatcgt tggcgaggat cccggggaac cgcttcgtga
     3301 tcccccgcat ctctacggca ggcggctcgg gcatgtgggc gtggcgtctt cttcaatcaa
     3361 gacggcatcc cctcgtatcc tcgcatgcgg acgggatcac ggcaacaagg tctccgactc
     3421 ccgcgtgagg gaggaacgct ccgaaacgcc atggaaggct tcacggcacc caggatggag
     3481 cgcgttccca cagcagcccc catgcggggg caagcccccc atacggggga caggcggggg
     3541 cgcctcgggg aggggtcacc gatgggcgaa acatacggt
//
LOCUS       DASQGH010000397         9457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7491, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000397 DASQGH010000000
VERSION     DASQGH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7491"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /gene="mazG"
                     /locus_tag="VEW91_07780"
     CDS             complement(<1..644)
                     /gene="mazG"
                     /locus_tag="VEW91_07780"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HYM91513.1"
                     /translation="MTTRRPTFDDLVATMARLRGPGGCPWDREQTHRSLRPYLLEEAY
                     EALEAIDHGSPDALRQELGDLLLQVVFHAQMAAEAGAFTIEDVVAALVEKLIRRHPHV
                     FGDAQVGGARDVLVHWEAIKAQERTEGLHGGETETHGQSALSDLPRTLPALARAQRMQ
                     EQAARAGFAWPNLAAALDKVREELAELEAAAGGRSEGGAPGEMGDLLFAVANLPR"
     gene            complement(688..4032)
                     /gene="mfd"
                     /locus_tag="VEW91_07785"
     CDS             complement(688..4032)
                     /gene="mfd"
                     /locus_tag="VEW91_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HYM91514.1"
                     /translation="MALSGILPLLDRRREFLYVCEHLERGDSPWVMGPSGAGKALVVA
                     GLIAHFGGRAPAWLILTLGRDAAERLADDLAAFLPASAGRVHLLAPWESFSPEDPPSV
                     EAEGARQRILEALRRGLPQVIVASAGGVVHAASDPRWIDTVRVELAPGAPSDLGLVVQ
                     RLTAAGYERTDLVRRPGEVAVRGGLIDVYPSTEDHPVRIEWAGDEIESLRAFDPETQR
                     TVGALPRVAILPARPSGGAAATLLDLVPDAVCVLDEPDELERQARALHEQAIAAYRRA
                     AAAGRVPATAPIPAVPWDRIASALDARRTVALSTLHGSPRGSAVELSFGALESFAGQT
                     AALAGRLRDWAAAGRRVVVASRQAHRMIELLADHGVAAGLVEHLDDLPAAGSVCAVPQ
                     ALTGGFTLDDLVVVTDAEILGWRRRRRHLRWVRDGARISSWTELAPGDLVVHIHHGIG
                     VYRGLERLTVGGGERDYLHLEYAHADALYVPTDQINLVQRYVGVEGQAPQIHRLGGTE
                     WEREKRRVRERTREIARELLELYAARETRAGHVFAPDTPWQREMEEAFTYEETPDQWQ
                     AIQDVKRDMESSRPMDRLVSGDVGYGKTEVAVRAAFKAVMDGKQVAVLVPTTILAQQH
                     FTVFQERFAAFPIRVEMLSRFRTPREQKAIVDALRTGAVDVIIGTHRLLSRSVTFKDL
                     GLVIIDEEQRFGVTHKERLKQLRTQVDVLTLTATPIPRTLHMSLAGLRDLSVMETPPE
                     ARQPIRTAIREESPELIREAIQAELNREGQVYVIHNRVETIERAAARIRRLVPDARVA
                     VAHGQMPEERLERVMLDFLGGRCDVLVSTTIVEIGLDIPRVNTIIIEDAHTLGLAQLY
                     QLRGRVGRADRQAYAYLLYPRHVRLTPEAEQRLVAMREFVELGSGLRLAMRDLEIRGA
                     GNLLGPEQHGHLAAVGFDLYLRLLEEAVREARGEVVEEPPEVSIDLDVGAYLPDTYIR
                     SPAQRMAAYRQLAAAQTVEEGRAAIAELRDRYGPLPQPVQHLAEIIRLRALARRAGVA
                     SITRDRAGVLVRLSDQAARSGRMRALSDASRGRLELTPEGILLRTDGSGLAETLRRIG
                     EMLDVLAGPEKSTGGVDPAPRAPASR"
     gene            complement(4139..5917)
                     /locus_tag="VEW91_07790"
     CDS             complement(4139..5917)
                     /locus_tag="VEW91_07790"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYM91515.1"
                     /translation="MTPRFRVAMAQINTTVGDFEGNTRKIIERLEEAQALGADVVSFP
                     ELAITGYPPEDLLIKSDFVGENLVCLREIARHAGHTAAIVGFVDSGDHLYNAAAVLYE
                     GRVAGVYRKMRLPNYGVFDEKRYFQPGTECPVYIIHGVSVGVNICEDVWAPGGPMLAQ
                     SLAGALVSITLNSSPYSAQKWRLRQEMLQTRARDYAVAVAYNNLVGGQDELVFDGLSV
                     IVDAAGHVVARGPAFEEALVIADLDVEAIRGIRRYNPIRTSDALHGEGLNAPVFTLSR
                     EAPPDAESRRPLAGARVMEPPPDVEEVYRALVLGTRDYVRKNGFRTVVLGLSGGIDSS
                     LVACIAVDALGRESVKGVALPSQFTSEASRRYTRELAGALGIELLDLPVDPVVAAYTQ
                     VLAAPFAGRAQDVTEENLQARTRGNVLMALSNKLGWLVLTTGNKSEMSVGYATLYGDM
                     AGGFAIIKDVPKTLVYRLAGWRNARAGRAVIPQGVLERAPTAELRANQTDQDTLPPYE
                     VLDPILKLYVEDDRSAEEIVAQGFDPATVARVVAMVDHSEYKRRQAPPGIKITPKAFG
                     RDRRLPITNWYRGRRTAEAPTRPTAS"
     gene            complement(6060..7502)
                     /gene="glnA"
                     /locus_tag="VEW91_07795"
     CDS             complement(6060..7502)
                     /gene="glnA"
                     /locus_tag="VEW91_07795"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HYM91516.1"
                     /translation="MVGKEMTARDVVAYIKEHGIKMVDLKFVDVPGTWQHATIPASQV
                     DEKPFTKGIGFDGSSIRGFQAIEESDMLLMPDPATARPDPFTAIPTLSVVCNVNDPIT
                     QKPYSRDPRHVAQKAEEYLRASGIADVSYWGPEAEFYTFSNVQYEVLPHRMGYSIDSP
                     EGTWNTGAAGLGSRMRVKEGYFPVAPSDTLVDARNEMTIELEKWGIEVEMQHHEVGHA
                     GQGEIDMKYAPLTRMADSLLSYKYIVKNVARRHGLTATFMPKPLFGDNGTGMHVHQSL
                     WKGGQNVFYREGGYAELSQEALYYIGGVLTHVDALMGLCACTTNSYRRLVPHYEAPVN
                     IAFSQRNRSAAVRIPMYFSGPGAAASKRIEFRCPDATCNPYLAFAAMLMAGLDGIKRR
                     TDPVRAGFGPLDKNTYELEPEDAAKIKNVPGSLSAALDALEADHEFLLEGDVFTRDLL
                     DTWLGYKRTREVQEVNIRPVPYEFFLYYDV"
     gene            complement(7699..8091)
                     /locus_tag="VEW91_07800"
     CDS             complement(7699..8091)
                     /locus_tag="VEW91_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM91517.1"
                     /translation="MRCQVRRRRPSSPSSGRWGHLAFANTNFLQRASVTSGTTLPERY
                     PELFKTGTGGTANYPSLREVVKTFDDTHEALMRAVAEAKLDASGEGPHGLWKNYGEMF
                     SFANAHRWYHIGKITSLRALLGKPRLFG"
     gene            complement(8195..9346)
                     /locus_tag="VEW91_07805"
     CDS             complement(8195..9346)
                     /locus_tag="VEW91_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM91518.1"
                     /translation="MRTRVVVLGAGFGGLELTTILSDAVGDALDLVLIDKGDAFVFGF
                     SKLDVMFGRLIPAEARHPYRDIVKPGVRFLQSTVRSFDPVGRRVETDGGTFDADVLVV
                     ALGADMDPAATPGLVEGGNEFYSVAGAEALRDVLPRFEGGTAIVGVTSTPFKCPPAPS
                     EAALLLHDYLTTRGRRGATEISVVMPFGTPIPPSPASSQAILVAFAERGIRFVPDRLV
                     RALDPGRKVAVLNDGTEMPYDLFLGVPVHRVPAVVEASGLAVGGWVPVNPKTLETRFP
                     GVYAVGDVTSVGTPKAGVFAEGAARVVATALIAQLRGGPSPGPYTGTGSCYVEFGHAL
                     VGRVDVDFLSGPKPTGTFQEPSAALAAEKALFGSSRLQRWFGRPPADRG"
BASE COUNT         1398 a         3379 c         3208 g         1472 t
ORIGIN      
        1 cggggcagat tggcgacggc gaataacagg tcgcccatct ccccgggagc cccaccctcg
       61 ctccgtcccc cggctgccgc ctccagctcc gccagttcct cccgcacctt gtcgagcgcc
      121 gccgccagat tcggccaggc aaaccctgcc cgggcggcct gctcctgcat ccgctgcgcg
      181 cgcgcgaggg ccggcagcgt tcgcggaagg tcggagagcg cgctttgccc gtgggtttcg
      241 gtctccccgc catggagccc ctcggtccgc tcctgcgctt tgatggcctc ccagtgtacc
      301 aggacgtcgc gcgcgccccc gacctgggca tccccaaaca cgtgggggtg ccgccggatg
      361 agcttttcca cgagcgcggc gaccacatcc tcgatggtga acgccccggc ttctgcggcc
      421 atctgtgcgt ggaacacgac ctggagcagc aagtccccca gttcctggcg gagcgcgtcg
      481 ggcgacccgt ggtcgatcgc ctcgagtgct tcgtacgctt cttcgaggag gtacggccgg
      541 agcgaccggt gcgtctgctc gcggtcccac ggacatcccc ccgggccgcg gaggcgcgcc
      601 atggtggcga ccaggtcatc gaaggtcggc ctgcgagtcg tcaaggcggt tcctcccaga
      661 acgtgattga gatcgccgcg gcccggccta ccggctcgcc ggcgcgcgcg gtgccgggtc
      721 gacgccgccg gtcgatttct ccggcccggc caacacatcg agcatctcgc cgatccggcg
      781 gagtgtctcc gccagcccgc tgccgtcggt gcggaggagg atcccctccg gggtgagctc
      841 gagccgtccg cgggatgcat ccgacagggc gcgcatccgg ccgctcctgg ccgcttggtc
      901 ggacagtcgg accagcaccc cggcccggtc tcgcgtgatc gacgcgaccc cggcccggcg
      961 ggccagagcc cggaggcgga tgatctccgc gaggtgctgg acgggctgcg ggagcggccc
     1021 gtaccggtcg cgcagctctg cgatcgccgc gcggccttcc tcgaccgtct gggcggccgc
     1081 cagttgccgg taggccgcca tgcgctgcgc gggcgaccgg atgtatgtgt cgggcaggta
     1141 ggcgcccacg tccagatcga tagacacctc gggcggttcc tcgacgacct ccccgcgggc
     1201 ctcccggacc gcttcttcga ggaggcgcag gtagaggtcg aaccccacgg ccgcgaggtg
     1261 cccgtgctgc tccgggccca ggaggttccc cgccccgcga atctccagat cccgcatcgc
     1321 cagccgcagc ccggatccga gctcgacgaa ctcgcgcatc gcgacgaggc gctgctcggc
     1381 ctccggggtg agccgcacat gccgggggta gaggaggtac gcgtacgctt gacgatccgc
     1441 ccgccctacg cgcccacgga gctggtagag ctgggccagg ccgagggtgt gggcgtcctc
     1501 gatgatgatg gtgttcaccc gcgggatatc gaggccgatt tccacgatgg tcgtgctcac
     1561 cagcacgtcg caccgcccgc cgaggaagtc gagcatcacc cgctccaggc gctcctcggg
     1621 catctgcccg tgggccaccg ccacccgcgc gtccggcacc agccggcgga tccgggcggc
     1681 ggcgcgctcg atggtctcga cccggttgtg gatgacgtag acctggccct cacggttgag
     1741 ctcggcttgg atggcttcgc gtatgagctc cgggctctcc tcccggatcg cggttcggat
     1801 cggctggcgg gcctcgggcg gcgtttccat caccgacagg tcgcgcaacc cggcgaggga
     1861 catgtggagg gtgcggggga tcggcgtcgc ggtcagcgtg aggacgtcga cctgggtccg
     1921 gagctgcttc agccgctcct tgtgcgtgac gccgaaccgc tgctcctcgt cgatgatgac
     1981 gaggccgagg tccttgaagg tgaccgaccg gctgaggagc cggtgcgtcc cgatgatcac
     2041 atcgaccgcg ccggtccgga gggcgtccac gatcgccttt tgctcccgcg gcgtgcggaa
     2101 ccggctcagc atttcgaccc ggatggggaa ggcggcgaac cgttcctgaa aaaccgtgaa
     2161 atgctgttgc gccaggatcg tggtggggac gagcacggcg acctgttttc cgtccatcac
     2221 cgccttgaac gccgcgcgga cggcgacctc ggtcttcccg taccccacgt cgccgctcac
     2281 caggcggtcc atcggccgcg aggactccat gtcccgcttc acgtcctgga tcgcctgcca
     2341 ctggtcggga gtttcctcgt aggtgaacgc ctcctccatt tcgcgctgcc acggggtatc
     2401 cggggcaaac acgtggccgg cccgggtctc gcgcgccgcg tacagttcga gcagttcccg
     2461 ggctatctcc cgggtccgct cgcggacccg ccgcttctcc cgttcccact cggttccgcc
     2521 gagccggtgg atctgcgggg cttgtccctc gacgccgacg tagcgctgga cgaggttgat
     2581 ctggtcggtc gggacataga gggcgtcggc gtgcgcgtat tcgagatgca ggtagtcgcg
     2641 ctccccgccg ccgaccgtca ggcgctcgag gccccggtac acaccgatgc cgtggtggat
     2701 gtggacgacg agatcgcctg gagcgagctc cgtccagctg gagatccggg cgccgtcgcg
     2761 gacccaccgc agatggcgcc ggcggcgccg ccagccgagg atctccgcgt cggtcaccac
     2821 gaccaggtcg tccagggtaa agcccccggt cagcgcctgg gggacggcgc acacgctccc
     2881 cgccgcgggg aggtcgtcaa gatgctccac gagcccggcg gccaccccgt ggtcggcgag
     2941 caactcgatc atccggtgcg cctgccggct ggcgacgacg acccgccggc ccgcggccgc
     3001 ccagtcgcgt agccgcccgg cgagcgcggc cgtctggccg gcgaacgact ccagggcgcc
     3061 gaacgaaagc tccacagccg atccccgcgg cgacccgtgg agcgtggaga gggccaccgt
     3121 ccggcgggcg tcgagcgcgg atgcgattcg gtcccacggc acggcgggga tcggcgcagt
     3181 ggccggcacc cgacctgctg cggccgcccg ccggtacgcg gcgatggcct gctcgtgcag
     3241 ggctcgcgcc tggcgctcaa gttcgtccgg ttcgtcgagg acgcatacgg catccggcac
     3301 gaggtccagc agcgtcgcgg cggccccccc gctcggtcgg gccggaagga tcgccacccg
     3361 cggcagtgcg ccgactgtcc gctgggtctc cggatcgaag gcgcggagcg attcgatctc
     3421 gtcgcccgcc cactcgatcc tgaccggatg gtcctccgtg gagggataga cgtcgatcaa
     3481 ccctccccgc accgcgacct ctcccggccg gcgcaccagg tcggtgcgct cgtacccggc
     3541 cgctgtgagg cgctggacca ccaggccgag atcgctgggg gcgcccgggg ccagctctac
     3601 ccgcacggtg tcgatccagc gcgggtcgga ggccgcatgc accacgccgc cggccgaggc
     3661 gacgatcacc tggggcaggc cccggcgcag cgcctccagg atgcgctggc gcgctccttc
     3721 ggcctccacc gaaggagggt cctcggggct gaatgattcc cacggcgcca gcaggtgcac
     3781 ccggccggcg ctcgccggca ggaacgccgc gaggtcatcg gccaggcgct ccgcggcatc
     3841 gcgtccgagg gtcaggatga gccaggccgg ggcccggcct ccgaagtgcg cgatgagccc
     3901 ggccaccacc agcgcctttc cggcgccgct cgggcccatg acccaggggg agtctccccg
     3961 ctcaagatgt tcacagacgt agagaaactc ccgtctgcga tccagcagcg ggagtatccc
     4021 ggacagcgcc atgagatgcc tccgcccgac cattgtacga agggaaggaa aatgcgtcaa
     4081 ccgacctgcg gggcacgggc acgccgggga gaccgcgccg cgacgcgggt cgagcgggct
     4141 acgatgcggt ggggcgggtc ggggcttccg cggtccggcg gccgcggtac cagttggtga
     4201 tcggcagccg ccgatccctg ccgaacgcct tgggggtgat cttgatcccg ggcggggcct
     4261 ggcggcgctt gtactcgctg tggtccacca tggcgaccac ccgggcgacc gtggcggggt
     4321 cgaacccctg cgcgacgatc tcctcggccg accggtcgtc ctcaacgtag agcttgagga
     4381 tcggatcgag cacctcgtac ggcggcaggg tgtcctggtc cgtctggttc gcccgcagtt
     4441 ccgcggtcgg cgcgcgctcg aggaccccct gcggaatcac cgcccggccc gcccgggcgt
     4501 tgcgccaccc ggccaggcgg tagacgagcg tcttgggcac gtccttgatg atcgcgaacc
     4561 ccccggccat gtctccgtag agggtggcat agcccacgct catctcgctc ttgttgccgg
     4621 tcgtcagcac gagccagccc agcttgttgg ataaggccat gagcacgttt ccgcgggtgc
     4681 gggcctgcag gttttcctcg gtgacgtcct gcgcccggcc cgcaaacggc gccgccagca
     4741 cctgggtgta tgcggccacc acggggtcga ccggcaggtc caggagttcg atcccgaggg
     4801 cgcccgccag ttcccgcgtg tagcgccggc tcgcctccga cgtgaactgg gaggggagcg
     4861 cgacgccctt gaccgactcc cgcccgagcg catcgacggc gatgcacgcc acgagcgagg
     4921 agtcgatccc gccgctcagc ccgagcacca cggtccggaa gccgttcttg cgcacgtagt
     4981 cgcgcgtgcc gagaacgagc gcccggtaca cctcctcaac gtctggaggc ggctccatca
     5041 cccgggcccc cgcaagcggc cggcggctct ccgcatccgg gggggcctcg cgggagaggg
     5101 tgaagaccgg ggcgttcagg ccctcgccgt gcagggcatc ggacgtgcgg atcgggttgt
     5161 accggcggat cccgcggatc gcctcgacgt ccaaatcggc gatgacgagc gcttcctcga
     5221 acgcgggccc gcgggcgacc acgtgcccgg ccgcatccac gatcacgctg agcccgtcga
     5281 agaccagctc gtcctgcccg ccgacgaggt tgttgtacgc caccgccacg gcgtagtcgc
     5341 gcgcgcgggt ttgcagcatc tcctgccgca ggcgccactt ctgggcggag tagggggacg
     5401 agttgagggt gatgctcacg agcgccccgg ccagggactg cgccagcatc ggcccgcccg
     5461 gggcccagac atcctcgcag atgttcacgc ccacggagac gccgtggatg atgtacaccg
     5521 ggcactcggt ccccggctgg aagtaccgct tctcgtcgaa gacgccgtag ttcggcagcc
     5581 gcatcttccg atacacgccg gcgacccgcc cttcgtacag caccgccgcg gcgttgtaga
     5641 ggtgatcccc ggagtcgacg aacccgacga tcgccgccgt gtggccggcg tgacgcgcga
     5701 tctcacggag gcagaccagg ttctcgccga cgaaatcgga cttgatcagg aggtcctcgg
     5761 ggggatagcc cgtgatcgcc agctccggga acgagaccac atcggccccg agcgcctggg
     5821 cttcctcgag gcgctcgatg atcttccggg tgttcccctc gaagtccccg acggtcgtat
     5881 tgatctgggc catcgcgacc cgaaaccgcg gtgtcatcgc agatcccacc gcatagtatt
     5941 atacaccatg cgcctgcgcc ccagccgatc gccgcgcgcc accccgaaaa accccgggag
     6001 ccacgcctgc gcaggacgtg gctcccgagg accccacccg gcgcgcgccg ggagcggcgt
     6061 cagacgtcgt agtacagaaa gaactcgtag gggaccggac ggatattgac ctcctggact
     6121 tcccgcgtcc gcttgtaccc gagccaggta tccagcagat cccgggtgaa cacatcgcct
     6181 tccagcagga actcgtggtc cgcctcgagc gcatcgagcg ccgcggacag cgaccccggg
     6241 acgttcttga tcttcgcggc gtcctcgggc tccagttcgt aggtgttctt atcgagcggg
     6301 ccgaatccgg ctctgaccgg atccgtcctc cgcttgatgc cgtccaaccc ggccatcagc
     6361 atcgcggcga acgccaggta cgggttgcag gtggcatcgg gacaccggaa ctcgatccgc
     6421 ttgctggccg ccgcccccgg gccggagaag tacatcggga tgcggaccgc cgcgctccgg
     6481 ttgcgctggc tgaacgcgat gttgaccggt gcttcgtagt gcggcaccag gcgccggtag
     6541 gagtttgtcg tgcacgcgca cagtcccatc agcgcgtcca cgtgggtcag caccccgccg
     6601 atgtagtaga gggcctcctg gctcagctcg gcgtagccgc cctcgcggta gaagacgttc
     6661 tggcccccct tccagaggct ctgatgcacg tgcatgcccg tcccgttgtc cccgaagagc
     6721 ggtttgggca tgaaggtcgc ggtcagcccg tggcgccggg ccacattctt gacgatgtac
     6781 ttgtaggaca gcaggctgtc cgccatccgg gtgagcggag cgtacttcat gtcgatctcg
     6841 ccctggcccg cgtggccgac ctcgtgatgc tgcatttcca cttcgatgcc ccacttttcc
     6901 agttcgatcg tcatctcgtt gcgggcgtcc acgagggtgt cggacggcgc gacagggaag
     6961 tacccttcct tgacccgcat ccggctgccc aggcccgccg cgccggtgtt ccaggtgccc
     7021 tccggcgagt cgatgctgta ccccatccgg tgcgggagca cctcgtactg cacgttgctg
     7081 aaggtgtaga attccgcctc gggcccccag tagctcacat cggcgatgcc cgacgcccgg
     7141 agatactcct cggccttttg agcgacgtgg cgcggatccc gcgagtacgg cttctgggtg
     7201 atcgggtcgt tcacgttaca gacgaccgac agggtcggga tcgcggtgaa cggatccggc
     7261 cgcgccgtcg ctggatcggg catcagcaac atgtcgcttt cctcgatcgc ctggaagccg
     7321 cggatgctgc tgccgtcgaa cccgatgccc ttggtgaacg gtttctcgtc cacctggctg
     7381 gccgggatcg ttgcgtgctg ccaggtcccc ggtacgtcga cgaacttgag gtccaccatc
     7441 ttgatgccgt gctccttgat gtacgcgacg acgtcgcgcg ccgtcatctc cttcccgacc
     7501 attctccacc tccgcgtccc ggccagggac cgatttgccc gctcgaaggc acaaaaaatg
     7561 cctcccggcg ccctcatccg cgcccgggag gcttcacggc ctccggcacc gctcagtacc
     7621 cgagggaata tatatcacgc gagggtgggg aagtcaacac tctcggacgg cccccgatac
     7681 ggccggcggg cgcccgcgct agccgaacag cctgggcttg ccgagcagcg cgcggagcga
     7741 tgtgatcttg ccgatgtggt accagcggtg agcgttggcg aacgagaaca tctcaccgta
     7801 gttcttccac agcccgtgcg gcccttcgcc cgaggcgtcc agtttggcct ccgccaccgc
     7861 gcgcatcagc gcctcgtggg tgtcgtcaaa cgtctttacg acctcgcgca acgacgggta
     7921 gttggctgtt ccgccggtcc ctgtcttgaa cagctcagga taccgctccg gcagggtggt
     7981 gccggaggtg acgctcgcgc gctgcaagaa attcgtgttg gcgaaggcca gatgccccca
     8041 cctgccagat gacggggaag agggtcggcg ccggcggacc tgacagcgca cgactacgcc
     8101 ttactccgaa tccgtccgtg ccacccggta ctgattcagc tgacacgtgc cactgggagg
     8161 atggagttcg tgcgctgagg gcggtaccct ccgctcagcc gcggtcggcg ggcgggcgcc
     8221 cgaaccagcg ctggagccgg ctcgagccga acagggcctt ttcggcggcc agcgccgcgg
     8281 atggttcctg gaaggtcccc gtgggtttgg ggccgctcag aaagtccacg tcaacgcgcc
     8341 cgaccagggc gtgtccgaac tcgacatagc aggagcctgt tcccgtgtag ggcccgggtg
     8401 acggcccgcc gcggagctgc gcgatgagcg cggtcgccac cacgcgcgcc gccccttcgg
     8461 cgaagacacc ggccttgggg gtgcccacgc tcgtcacgtc gcccaccgcg tacaccccgg
     8521 gaaaacgcgt ctccagcgtt ttcggattca ccggcaccca gccgccgacg gcaagcccgg
     8581 acgcctcgac caccgccggc acgcggtgca ccggcacgcc gaggaacagg tcgtacggca
     8641 tctcggtccc gtcgttcagc accgccacct tccggcccgg gtcgagggcg cgcaccagcc
     8701 ggtcggggac gaaccgaatg ccgcgttccg cgaaggcgac gagaatcgcc tgcgaggatg
     8761 cgggcgacgg gggaatgggc gtgccgaagg gcatcaccac cgagatttcg gtggccccgc
     8821 gccgcccgcg ggtggtgagg tagtcgtgca gcagcagcgc cgcctcgctg ggggccgggg
     8881 ggcacttgaa gggtgtcgat gtcacgccga cgatggccgt gcccccctcg aaccggggca
     8941 gcacgtcgcg cagcgcttcg gcgcccgcga ccgagtagaa ttcgttgccg ccctccacga
     9001 gcccgggggt ggccgccggg tccatgtcgg cgccgagcgc caccaccaag acgtccgcgt
     9061 cgaacgtccc tccgtcggtc tccacacgcc ggccgaccgg gtcgaaggac cgcaccgtag
     9121 actggaggaa ccgcaccccc ggcttgacga tgtcgcggta aggatgacgc gcctccgccg
     9181 gtatcagccg cccgaacatc acgtcgagct tcgagaagcc gaagacgaac gcgtcaccct
     9241 tgtcgatgag cacgaggtcg agcgcgtcgc cgaccgcgtc tgaaagaatc gtcgtcagct
     9301 cgaggccgcc gaagccggcc cccagtacta ccacgcgtgt acgcatttct cgatcagccg
     9361 ccacaaaacc gtatttgact catgtccggc ttgccccgat cgagctcttg cggaacccag
     9421 tctcactacc gagggtccgg acctccggag gtgcgga
//
LOCUS       DASQGH010000398         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41336, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000398 DASQGH010000000
VERSION     DASQGH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41336"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VEW91_07810"
     CDS             <1..922
                     /locus_tag="VEW91_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYM91519.1"
                     /translation="PKQAGGKLFSDPLARLAFWLFLVLSTPVALHHQFLDPGIPPAWK
                     FVHAILTYAVFFPSMMTAFNVIASLELGARARGGRGLFGWMRALPWGDPSFATQNLAM
                     ILFAFGGIGGLVNASYNVNLVVHNTLFVPAHFHLTVATAVTLTFMGVLYWLLPHLTGR
                     KLWAPKLALAQAWTWFVGMLIMSGAMHLVGLDYGVPRRAMLGSASYAEAAWKPLLADA
                     AVGGVILWISVTLFFIVVVGTLVAGARSEQRVEMPVTEPYQAAPVPAWLDNWRLWISF
                     AVVLIVLAYGPVLINLIATAQLNAQGLRVW"
     gene            978..2429
                     /locus_tag="VEW91_07815"
     CDS             978..2429
                     /locus_tag="VEW91_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HYM91520.1"
                     /translation="MGAAPGSVDETARPAQRKRSAALSSLAAAGLLTALKIVAGVLTG
                     SLGILSEAAHSSLDLLAAAVTYASVRISEQPADPSHPFGHGKVEHLSAFVQTALLIVT
                     SVWIVFEAIRRLFFFDVHVDPSVWAFGVLSLSAAVDLARSRSLARAARAYQSQALEAD
                     ALHFSTDVYGTCAVMLGLLLVSIAGRPHLAGLRYADPLAALLVAGFSVYVGTRLGNQS
                     VGALLDAAPEGTLERIAEIVSRIPGIRAFERIRARQSGAKLFVDLRLTLQSNIPLEHA
                     EMIENTVTAKIREQYPGADVMIDTTPGSPSPDNLVERIRSVAHQENFHVHDITVIDVK
                     GQTLINLDLEVDPTLPLTTAHERATALETLVRRSVPGVHDINVHIEPRRESVATAEDA
                     PGMQADMEGALREAVRSVPEVIDCHSIEVHRADEEMVVTVHCTLRPGLSVERAHDITE
                     QLDLEIRERFHQNMTVNIHAEPATGGARPPSGS"
BASE COUNT          395 a          878 c          828 g          447 t
ORIGIN      
        1 gccgaagcaa gccggaggga agctgttcag cgatccgctc gcgcgcctcg cgttctggct
       61 cttcctggtg ctctccacgc cggtcgccct gcatcaccag tttctcgacc cggggattcc
      121 gcccgcctgg aaattcgtgc acgcgatcct cacgtacgcg gtgtttttcc ccagcatgat
      181 gacggcgttc aacgtgatcg cctcgctgga actcggggcc cgggcgcgcg gcggccgcgg
      241 cctgtttgga tggatgcgcg cgctcccgtg gggtgatccc tcgttcgcca ctcaaaacct
      301 ggcgatgatt ctgttcgcgt tcggcggcat cggcgggttg gtgaacgcct cgtacaacgt
      361 caacctggtc gtccacaaca cgctgttcgt gcccgcccac tttcacctca cggtcgccac
      421 cgcggtgacc ctgacgttca tgggcgtcct gtactggctg ctgcctcacc tgaccgggcg
      481 gaagctgtgg gcgccgaagc tggcgctcgc tcaggcctgg acgtggttcg tcgggatgct
      541 catcatgtcc ggcgcgatgc atctcgtcgg gctggactac ggcgtccccc gccgcgccat
      601 gctcgggtcg gcctcgtacg cggaggcggc gtggaagccg ttgctcgcgg acgcggcggt
      661 cggcggcgtg atcctctgga tcagcgtgac gctgttcttc atcgtggtcg tgggcacgct
      721 cgtggcgggc gcccggtcag aacaacgcgt cgagatgccg gtgaccgagc cgtatcaggc
      781 cgccccggtg ccggcgtggc tcgacaactg gcggctgtgg ataagcttcg ccgtggtgct
      841 gatcgtgctc gcgtacggac ccgtcctgat caacctgatc gcgacggcac agttgaacgc
      901 ccaggggttg cgcgtctggt agccagcgcc gcgaccgtag tgctgaacgc cccttgatca
      961 acggggtaga tggaggtatg ggagcggctc ccggcagcgt cgacgagacg gccaggccgg
     1021 cgcagcggaa acgatccgcg gccttgagct ctcttgccgc cgccgggctt ctgacggcgc
     1081 tcaagatcgt ggcaggcgtg ctgacgggaa gcctcggcat cctctctgag gccgcccact
     1141 ccagcttgga cctgctcgcc gcggcggtca cgtacgcatc cgtgcggatt tccgaacagc
     1201 ccgccgaccc gtcgcatccc tttggacacg ggaaagtgga gcatctctcg gcgttcgtcc
     1261 agaccgcctt gctcatcgtg accagcgtct ggatcgtctt cgaggcgatc cgtcggctgt
     1321 tcttcttcga cgtgcatgtg gatccgtccg tgtgggcgtt tggcgtgctc agcctctccg
     1381 cggccgtcga cctcgcccgg tcccggtccc tggcccgtgc cgcccgcgcc taccagagcc
     1441 aagcccttga agccgatgcg ctgcacttct ccaccgacgt ctacggcacg tgcgcggtca
     1501 tgctggggct gctcctcgtc tccatcgccg ggaggccgca cctcgccggg ctgcggtacg
     1561 ctgacccgct cgcggcgctg ctcgtcgccg gattcagcgt ctatgtcggc acgcggctcg
     1621 gcaatcaaag tgtgggggcg ctgctcgatg ccgcaccgga aggcaccctg gagcgcatcg
     1681 ccgagatcgt gtcccgcatc cccgggatcc gcgcattcga gcgcatccgg gcgcgccaga
     1741 gcggggcaaa actcttcgtc gacctcaggc tgaccctgca gagcaatatc cctctcgagc
     1801 acgcggagat gatcgagaac acggtcacgg cgaagatccg cgagcagtac cccggcgccg
     1861 acgtgatgat cgacaccacg cccggcagcc cgtctcccga caaccttgtc gagcgcatac
     1921 gctcggtcgc gcaccaggaa aatttccacg tccacgacat tacggtgatc gacgtgaagg
     1981 ggcaaacgct catcaatctc gacctcgagg tcgacccaac cctgccgctc acgacggcgc
     2041 acgagcgcgc cacggcgctg gaaacgctcg tgcggcggtc ggtgcctggt gtccacgata
     2101 tcaacgtcca catcgaaccc aggcgcgaga gcgtggcgac cgccgaggac gccccgggga
     2161 tgcaggccga tatggaaggc gcgctgcgcg aagccgtccg gagcgtcccc gaggtgatcg
     2221 actgtcactc gatcgaggtg caccgggccg acgaagagat ggtcgtgacg gtgcactgca
     2281 cgctccgccc cggcctgtcc gtggaacggg cccacgacat caccgagcag ctggatctcg
     2341 agatacgaga acgcttccac cagaacatga cggtcaacat tcacgccgaa ccggcaaccg
     2401 gcggcgcgcg gccgccgtca ggttcctgat ctcgttcacc gctacctcag cccagattcc
     2461 gcacctgctc gcgaagtcgg cgtcaccgcg ccggcggacg atcattgggg agggacaacg
     2521 cggggctcta acgggttgcg tcaaggta
//
LOCUS       DASQGH010000399         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18759, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000399 DASQGH010000000
VERSION     DASQGH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18759"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VEW91_07820"
     CDS             <1..874
                     /locus_tag="VEW91_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91521.1"
                     /translation="LGLRGGELDGIYGVKDEAWVDKMQTVPKVLVDIFGVGESTVIYM
                     NQTVRPLSLKPVRQAIAYALNRDEFLALIGKKIGQKIYAPLAPFTIGALTEQEVVSKG
                     LDYKTNIERAKGLLAEAGVSGGFAVDLVTSQLGDYKIHYESMQAQLSRVGITVRLRVV
                     DHPSYHALIRKDANPIVIYIAFRPTADIYLRSFYHSASTVVTGAHPDTNLAHYAGVDD
                     VIDRARVEISAARQIELWKEAQIKILQDMVAYPVSYVNQVYARSTALDYGHGLKSVLA
                     LYPGIDETTRLRRG"
     gene            899..1837
                     /locus_tag="VEW91_07825"
     CDS             899..1837
                     /locus_tag="VEW91_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91522.1"
                     /translation="MVRYLVSRLLLALPIFLGVVTIVFVVVRILPGDPAQAALGDYAS
                     RDAVAALRQRLGLDRPIPLQYGSYLVGLARGDLGKSLITGASVRDEIAHNLPYTLQLT
                     VLAMIIGALTGVPVGVLNALTRNRLPDSIGRVLSLFGLSVPAYYLGILLILLFAIEWR
                     MLPAVGAGDLSDITDSVRHLVLPALTLGLVMAAAVARFTRSTVLNTLGQDFVRTARAK
                     GLPGRVVTVRHTLRAALVPIVSLAGLWSIGLIGDSVTTEIVFARPGLGSMMVGTMLQR
                     DYTSLQSVMVVYTAFVVLINLITDVAYGFIDPRIRY"
     gene            1851..2729
                     /locus_tag="VEW91_07830"
     CDS             1851..2729
                     /locus_tag="VEW91_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91523.1"
                     /translation="MGAASAGTEAMLGRTQGQRAWATLLRNRVSLAGGVLAIGIAAAS
                     TAAPLVTSWSPLAQDPFHRLRPPGGQHLLGQDTFGRDVFARILYGGRVSLLVGVGAVA
                     LGGVVGTALGLAAGYYGGRTENFLMRFVDILMAFPSLLLGLIVLAVLGPGLGKMIFAI
                     GIVLSPPFARIAHGTTLSLVRQEFVDAARAVGAGTWRILTGHILRNVMGEIVVLSGLL
                     AAGAIRIEASLSFIGLGVSPPTPTWGNMIREGTPFLIDSPWLSVAPGIAILLAVLAFN
                     LVGDALRDVIDPRLRV"
     gene            2741..3310
                     /gene="xpt"
                     /locus_tag="VEW91_07835"
     CDS             2741..3310
                     /gene="xpt"
                     /locus_tag="VEW91_07835"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HYM91524.1"
                     /translation="MDTLRARILSEGRNLGGGILKVDGFINHQIDPLLMNECGRELAR
                     RFRPAGATKVLTAEISGIAPALSTAQHLGVPVLYARKTRPITMPDQVLLTAVPSHTKE
                     RTVELIVSPEYLAHEDRVLIVDDFLATGHTILGLARLAGAAGARLVGIGVVIEKAFEG
                     GRAALEPLGVPIESLAIVTDMSGGRIVLA"
     gene            complement(3384..3680)
                     /locus_tag="VEW91_07840"
     CDS             complement(3384..3680)
                     /locus_tag="VEW91_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91525.1"
                     /translation="MKRKEFLVTAAGVAATLAASTAALAQTTAPTQPQNPTRGETGSA
                     RNIKKVRYMVERLIDELQHDQRDYGGYRVKAIAQLQQARADLDQALQWDATHPQ"
     gene            complement(3918..4241)
                     /locus_tag="VEW91_07845"
     CDS             complement(3918..4241)
                     /locus_tag="VEW91_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91526.1"
                     /translation="MRTISSIVGLALAIALVAGAASAQTTQLPVTPQPLPPAPPAPNY
                     YYGPVVQHPTGGQFPYVASLKPFSAETNNMSLDGYLRQLVFQQTGKWISPADAEHVVQ
                     MQKAQ"
     gene            complement(4302..>4765)
                     /locus_tag="VEW91_07850"
     CDS             complement(4302..>4765)
                     /locus_tag="VEW91_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HYM91527.1"
                     /translation="EDFYGGGALMPLGGEVAGHKGYGLGLASALVGGLSMIDDPNPTR
                     VGSPSAEPHPGPRGWMGGVFLLVVDPAAFGNADHYRAMVGGTLGAAKRMPPAPGRDAV
                     LLPGEPEVLSRKRRAADGIELPEAIWKDLAALAVRFNVPLPSARPAEQGMG"
BASE COUNT          709 a         1654 c         1577 g          825 t
ORIGIN      
        1 gctgggcctc aggggcggcg agctcgatgg gatctacggg gtcaaggacg aggcgtgggt
       61 ggacaagatg cagaccgtcc ccaaggtcct cgtcgacatc ttcggcgtgg gggagtcgac
      121 ggtcatctac atgaaccaga ccgtccgccc gctgagcctc aagccggtcc gccaggcgat
      181 cgcctacgcc ctcaaccggg acgagttcct ggcgctgatc ggcaagaaga tcgggcagaa
      241 gatctacgcg cccctcgccc cgtttacgat cggcgccctg accgagcagg aagtggtctc
      301 gaaggggttg gactacaaga cgaacatcga gcgggccaag ggcctgctcg cggaggccgg
      361 ggtgtccggc gggttcgccg tcgacctggt cacctcccaa ctcggcgact acaagatcca
      421 ctatgaatcg atgcaggccc agttgagccg ggtcggcatc acggtccgcc tccgcgtggt
      481 cgatcacccc agctatcacg cgctgatccg caaggacgcc aacccgatcg tgatctacat
      541 cgcgttccgc ccgaccgcag acatctacct ccgcagcttc taccactcgg cctcaaccgt
      601 cgtcaccggc gcgcatcccg acaccaactt agcccactac gccggggtcg acgacgtgat
      661 cgaccgcgcc cgggtcgaga tcagcgcggc ccggcagatc gaactctgga aggaagcgca
      721 aatcaagatc ctgcaggaca tggtcgccta tcccgtctca tacgtcaacc aagtgtatgc
      781 ccggtccacc gcgctggact atgggcacgg gctcaagtcc gtcctcgccc tgtacccggg
      841 gatcgacgaa acgacccgcc tccggagggg gtgaggcgcc cgacccaccc cggcgccgat
      901 ggtccggtat ctcgtcagtc gcctcctgct ggcgcttccg atcttcctgg gggtcgtcac
      961 gattgtgttc gtcgtcgtgc ggatcctgcc cggggatcct gcgcaggcgg cgctcggcga
     1021 ctacgcttcg agggacgccg tggccgccct ccggcaacgc ctgggcctgg accggccgat
     1081 tccgctccag tacggcagct acctggtcgg actcgcccgt ggcgacctgg gcaagtcgct
     1141 gatcacgggc gcttcggtcc gcgacgagat cgcccacaac ctcccctaca ccctccagct
     1201 gaccgtgctg gccatgatca tcggggccct gaccggcgtc ccggtggggg tcctcaacgc
     1261 gctcacccgc aaccgccttc cggactcgat cggccgggtc ctctcgctct ttggcctgtc
     1321 ggtcccggcg tactacctgg ggatcctgct catcctgctc tttgcgatcg agtggcggat
     1381 gctgccggcg gtcggtgcag gggatctctc tgacatcacc gacagcgtgc gccatctcgt
     1441 cctgcccgca ttgacgctcg ggctggtcat ggccgccgcg gtggcccgct ttacacggtc
     1501 gacggtgctc aacacgttgg gccaggactt cgtgcggacg gcgcgggcga aagggctccc
     1561 cgggcgggtc gtcacggtgc ggcacacgct gcgcgcggcg ttggtcccga tcgtctcgct
     1621 cgcggggctg tggagcatcg ggctgatcgg ggactcggtg acgaccgaga tcgtcttcgc
     1681 ccgccccggg ctgggcagca tgatggtcgg cacgatgctg cagcgtgatt acacgtccct
     1741 ccagtccgtc atggtcgtgt acaccgcgtt cgtcgtgctg atcaacctca tcaccgacgt
     1801 ggcgtacggc ttcatcgacc cccgcatccg gtactgaggg aggctcgagg atgggcgcgg
     1861 cgagcgcggg gactgaagca atgctggggc gcacgcaggg gcaacgagcc tgggcgacgt
     1921 tgctgcgcaa ccgggtctcg ctcgccgggg gcgtgctggc gatcgggatc gcggccgcat
     1981 cgaccgccgc gcctctcgtc acctcgtgga gccccttggc ccaggacccg ttccaccggc
     2041 tccggccgcc cggaggccag cacctcctgg ggcaggacac ctttggacgg gacgtcttcg
     2101 cccggatcct gtacgggggc cgcgtttcgc tgcttgtggg cgtcggcgcg gtcgcgctcg
     2161 ggggggtcgt gggaaccgcg ttgggcctgg ccgccggata ctacgggggc cggacggaga
     2221 atttcctcat gcggttcgtg gacatcctga tggcgtttcc cagcctcctg ctcggactga
     2281 tcgtgctggc ggtgttgggc ccgggcctcg gcaagatgat cttcgcgatc gggatcgtcc
     2341 tctcgcctcc gttcgcgcgg atcgcccatg ggaccacgct ctccctggtg cgccaggagt
     2401 tcgtcgacgc ggcgcgcgcg gtcggcgcgg gcacctggcg gatcctgacc ggccacatcc
     2461 tgcgcaacgt catgggcgag atcgtggtgt tgagcgggct cctcgccgcg ggagcgatcc
     2521 ggatcgaagc cagcctcagc ttcatcggcc tcggcgtctc accccccaca ccgacctggg
     2581 gcaacatgat ccgggagggc accccctttc tcatcgactc tccctggctg tcggtcgcgc
     2641 ctgggatcgc gatcctcctc gccgtcctcg cgttcaacct ggtgggggat gcgctccgtg
     2701 acgtcatcga ccctcggctt cgggtctgag aggaagtccc gtggacacgc tccgggcgcg
     2761 catcctgagc gaaggccgca accttggggg cggcatcctc aaggtcgacg ggttcatcaa
     2821 ccaccagatc gacccgctcc tcatgaacga gtgcggccgg gagctggccc gccggttcag
     2881 gcctgccggg gccaccaagg tgctcaccgc ggagatctcg gggatcgcgc cggccctcag
     2941 cacggcccag cacctcggcg tgccggtcct gtacgcgcgc aagaccaggc ccatcacgat
     3001 gccggatcag gttctgttga ccgccgtgcc ctcgcacacg aaggagcgca cggtcgagct
     3061 gatcgtctcc cccgagtacc tcgcccatga ggaccgggtc ctgatcgtag acgacttcct
     3121 cgcgaccggg cacacgatcc tcggcctcgc ccgcctcgcc ggggccgcgg gggcacggct
     3181 cgtcggcatc ggcgtggtga tcgagaaggc cttcgagggt ggacgcgcag cactggagcc
     3241 gctgggggtg ccgatcgagt cgctcgcgat cgtcacggac atgagcgggg gaaggatcgt
     3301 gctcgcgtag gacaggtcac agatcgggaa aacacggagc cgcggaggat cctccgcggc
     3361 tccgttcttc gctcaacctc gggttactgg ggatgcgtgg cgtcccactg gagggcctgg
     3421 tcgaggtcgg cccgcgcctg ctgcaactgg gcgatggcct tcacccggta cccgccgtag
     3481 tcgcgctggt cgtgctggag ctcatcgatg agccgctcga ccatgtagcg caccttcttg
     3541 atgttgcgcg cgctcccggt ctctccgcgg gtgggattct gcggctgcgt cggtgccgtg
     3601 gtctgcgcca gcgccgccgt cgacgcggcg agcgtcgcgg caacgcctgc tgccgtcacg
     3661 aggaattcct tgcgcttcat gtgcacatcg acctccttta gccgagtatg gctgttagag
     3721 cgaccgagcc ccgagcatag ttcccggggg atgtgtatgc ccacgaggca ccgcgcatag
     3781 cgtacatttc ccccgaagtt ccgtggactt tcgcgggaaa tttctggggc tgcggcctca
     3841 gacgtcaaga ggcgctcgcg aaaacgcaca ggggagggag cacgccccct cccctcaggc
     3901 cgggcgaacc tcggacgcta ctgggctttt tgcatctgca cgacgtgctc ggcgtcggcg
     3961 ggcgagatcc acttgcccgt ctgctgaaac accagttgcc gcaggtagcc atccagagac
     4021 atgttgttcg tctcagcgct gaacggcttc aggctcgcca cgtacgggaa ctgcccgccc
     4081 gtgggatgct gcaccaccgg gccataatag tagtttggtg ccggcggcgc cggcggaagc
     4141 ggctgcggcg tcacaggaag ttgggtggtc tgagctgacg ctgctcccgc aaccagtgcg
     4201 atggcgagcg cgagccccac gatcgaggat atggtccgca tgttcaccct cccgttgtgt
     4261 cgtgcgatct cagcgcgatg aacctgtccc ctccgtattc attaccccat tccctgttcc
     4321 gccggacgag cgctggggag cggcacgttg aagcgtactg caagggccgc cagatccttc
     4381 cagatcgcct cggggagctc gatgccgtcg gccgcccggc gctttcggct gagcacctcc
     4441 ggttcccccg gcagcagcac cgcgtccctt cccggcgccg gcggcatccg cttggccgcg
     4501 cccagcgtcc caccgaccat cgcccgatag tgatcggcgt tcccgaacgc ggcgggatcg
     4561 acgacgagca ggaacacccc gcccatccag ccccgaggac cgggatgcgg ctccgcgctg
     4621 ggggagccga cccgggtcgg atttggatcg tcgatcatgc tcaggccgcc aaccagcgcg
     4681 gatgccaggc ccaggccata gcctttgtgc cccgcgacct cgccgccgag cggcatcaac
     4741 gcgcctccgc catagaagtc ctcgg
//
LOCUS       DASQGH010000400         5577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24503, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000400 DASQGH010000000
VERSION     DASQGH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24503"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VEW91_07855"
     CDS             <1..1188
                     /locus_tag="VEW91_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM91528.1"
                     /translation="YKKFNLQGWGKAFGQAHKLNVRFQTTPDNDISPELLSWPKGKTE
                     WDVIVVGLPLFVAPLVGQNLLVDMTDFIKGYGEDKFVPTFLRDTKFARGNGWYWPMVP
                     FLGELIGIQINTKMYRAAGLVDAKGTPKPIPAWDLDAMIDYFRKLSVVSPLKVGLGLD
                     WDAEFGLHNYLAPLLGTRGTIYSKSDPRIIDLESPEAVKILTLYSRLQKENLLFGDIA
                     PTAAGDALTNFKAGLLPAHATTVSRAIESATNLGAENVSWLLWPGADRHGSITYTHNA
                     FIPKVSPNQKLAADFIREQVLSLPAQVWTFNNFGKLPSMREAYKHVKWFQKEAEATLA
                     VASISTFEPKYKGIAQLGELFTKETQQSILKGSDPAQALANIRKGIAEKHIDLTLLGY
                     TGA"
     gene            1259..2137
                     /locus_tag="VEW91_07860"
     CDS             1259..2137
                     /locus_tag="VEW91_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM91529.1"
                     /translation="MRARSWTAYVYLGPAAAFMAAFIAYPMLRSFYLSFTKYNYVYDP
                     RPTWAGLGTYAAVLQDSVFQTALQNQFKFGIPFFVIAFVLSLLVAATLMRVARGRTTL
                     QLAVILPMIIPPSLGAIVFLWLLEPGFGVVNAAWRALLHTRPPAWFLSPELALYVMVP
                     IFVWEELAFPVIIFLAGLQTISASLYDAARVDGASFWGEIRFVTLPSLRPTMVVASVY
                     LITQSLKMFDLPYVLTQGGPANATFTLYYYAWTRAFKFYEMGNGAAAAYITAVLILLF
                     SAGSSILLGERPGEAL"
     gene            2134..2961
                     /locus_tag="VEW91_07865"
     CDS             2134..2961
                     /locus_tag="VEW91_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM91530.1"
                     /translation="MRRLGPYAFLTAVAAAVVFPLVWMFVTSVKPEKEIFTFPITILP
                     HRLTVDNYVYVFTRLAGFRRFFVNSIAVTSISLLLILVLGALGGYPLARKEFPGKAEF
                     FGFILLVIAVPWVIYLIPTFVMEDALGLRNSWPGLILPYVALNLPWALLIMRSAFLAI
                     PQEIVDAASVDGAGEFQTWLRVLVPLTVPGLTTTFLIVFVFIWKEFLYAVTLMTESEW
                     QTLPVGIIQIRAELQSLVFNILSPTIIVALVPIFVVFILLRNFLVGGVLATGGFGKE"
     gene            3070..3975
                     /locus_tag="VEW91_07870"
     CDS             3070..3975
                     /locus_tag="VEW91_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYM91531.1"
                     /translation="MRLGVTVAHARVHYGDTPTLDQYGAFAEWAARMRFTGIELAAFT
                     AEQFVRDFGEAGALRQLGERCHALGLTINAFEAGFLRHMTVSEDAAVRRRGAADLDRA
                     VHVARALGSDLVYFHSAPHPSWTIEFKRLYDDFSPPVKVDVPAAFAWDQAWNQYVATI
                     SELARIAERAGVRLAMEIRPYEMVSNADGMHRLIDAVGSPALGVVFDTAHFLVQKELL
                     PIAIEKLGNRIFLVHLADNDGATDYHWAPGKGAVPWDGVLHALRKIRYAGFANIDVAG
                     TYDDIDAEIRAGRDFIANRLGLADR"
     gene            complement(3997..4815)
                     /locus_tag="VEW91_07875"
     CDS             complement(3997..4815)
                     /locus_tag="VEW91_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYM91532.1"
                     /translation="MKTSLYLAATGSRFWAASSGLALDDAVAKAAALGFDAVEIMPRD
                     VDDPDPARLRATAERAKLGVAAIASGFIAIERGLTFTHPNPEDRRRAVQAMRECMDAA
                     RRAGAPLVSIGIIRGKLQPGTTRAEAMRHLIDCIRECGRRAGDLGLVLAVEPGNRYET
                     DFIHTVTEGLELVGAVGLPSVRLMIDTFHMNIEEASIPGAIRQAAPYLAHVHFADSNR
                     RAPGWGHLDFPAVAATLREIGYRRTIGLEMTLAPDFEAAARQGVQFVRALFRTD"
     gene            complement(4812..>5577)
                     /locus_tag="VEW91_07880"
     CDS             complement(4812..>5577)
                     /locus_tag="VEW91_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91533.1"
                     /translation="DINTRLENAPRVDGRCIVGVGFHAAALAPVTAARHRAFGRGLLG
                     KALLRQKTYAEAVGLDLRDKRHWFPWFVAAALFAKPIRAEVARNTASLLFREGITTPE
                     AIQKQGWDKLVTILDRGGYVRYDFSTATKLLAIASALPGARLQQIVAEMSSPHIQTRL
                     MEIKGVGPKTVEIFLRELRGVAKIRLPLSREAREAARRLKLRLEGIDLRGMDFARLES
                     VLVRIWVEHCKRRLWSTCPAGPACGCAPRRDERFLR"
BASE COUNT          909 a         1891 c         1774 g         1003 t
ORIGIN      
        1 tacaagaagt tcaacctgca gggatggggc aaggcgtttg gtcaagcgca caagctgaac
       61 gtccggttcc agacgacacc ggacaacgac atctcccccg agctcctctc ttggccgaag
      121 gggaagacgg aatgggatgt catcgtcgtg gggctcccgc tgttcgtcgc gccgctcgtc
      181 ggccagaacc tgctcgtcga catgacggac ttcatcaagg gctacggcga ggacaagttc
      241 gtcccgacgt tcctgcggga caccaaattc gcccgcggca acgggtggta ctggccgatg
      301 gtcccgtttc tcggtgagct gatcggcatc cagatcaaca cgaagatgta ccgggccgcc
      361 gggctggtgg acgcgaaagg caccccgaag ccgattccgg cgtgggacct cgatgcgatg
      421 atcgactact tccggaagct gtccgtcgtt tcaccgctca aggtggggct ggggctcgac
      481 tgggacgccg aattcggcct gcacaattac ctggcgccgc tgttggggac ccgggggacc
      541 atttacagca agagcgaccc ccggatcatc gacctggaaa gccccgaggc ggtcaagatc
      601 ctgacgctct actcgcgcct gcagaaggag aacctgctct tcggcgacat cgctcctacc
      661 gcagccggcg acgccctcac gaacttcaag gcggggctgc tgccggcgca cgccaccacg
      721 gtctctcgcg cgatcgaatc ggccaccaac ctcggggcgg agaacgtgtc gtggctcctc
      781 tggccgggtg ccgaccggca cggatccatc acctatacgc acaacgcgtt catccccaag
      841 gtctcgccga accagaagct ggcggcggac ttcatccgcg agcaggtgct ctcgctcccg
      901 gcgcaggtgt ggacattcaa caacttcggc aagctgccgt cgatgcggga ggcgtacaag
      961 cacgtcaagt ggttccagaa ggaagccgag gccaccctgg cggtcgcgag catctccacg
     1021 ttcgagccca agtacaaggg catcgcccag ctcggggagc tcttcaccaa ggagacccag
     1081 cagtcgatcc tcaaggggag cgaccccgcc caggcgctcg cgaacatacg gaagggcatc
     1141 gcggagaaac acatcgactt gaccctgctc gggtataccg gggcctagcc ggcccccgaa
     1201 ggcctgtccg aggcgggagg ggactgcccc cttccgcctc cgcgggccgc gcgactcgat
     1261 gcgcgcccgg tcgtggaccg cctatgtgta cttggggccg gcggcggcgt tcatggcggc
     1321 ctttatcgcc taccccatgc tccggtcgtt ctacctcagc ttcacgaagt acaactacgt
     1381 ctacgatccc cgccccacgt gggcggggct ggggacgtac gcggccgtgc tgcaggactc
     1441 ggtcttccag accgcgctcc agaaccagtt caagttcggg atcccgttct tcgtcatcgc
     1501 gttcgtgctc agcctgctcg tcgccgccac gctcatgcgg gtcgcgcgcg gccggacgac
     1561 cctccagctc gcggtgatcc tcccaatgat catcccgccc tccctggggg ccatcgtgtt
     1621 cctctggctg ttggagcccg ggttcggggt cgtgaacgcg gcgtggcgcg cgctgctcca
     1681 cactcggccg ccggcctggt tcctctcgcc ggagctcgcc ctgtacgtga tggtgccgat
     1741 cttcgtctgg gaagagctcg cgtttcccgt gatcatcttc ctcgccggcc tgcaaacgat
     1801 ctcggcgagc ctctacgacg ccgcccgggt ggacggcgcc tcgttctggg gcgagatccg
     1861 gtttgtgacc ctccccagcc tcaggccgac catggttgtg gcgagcgtct acctgatcac
     1921 ccagtcgctg aagatgttcg atctgcccta cgtcctcacc cagggcggtc cggcgaacgc
     1981 caccttcacg ctctactact acgcctggac gcgggcgttc aaattctatg agatggggaa
     2041 cggagcggcc gccgcctata tcaccgccgt cctgatcctg ctgttcagcg cgggctcctc
     2101 gatcctcctc ggcgagcggc cgggggaggc gctgtgagac gcctgggccc ctacgcgttc
     2161 ctcaccgcag tggccgcggc ggtcgtcttt ccgctcgtgt ggatgttcgt gacctcggtg
     2221 aagcccgaga aggagatctt cacgttcccg atcacgatcc tcccccaccg gttgacggtc
     2281 gacaactacg tgtacgtctt cacccgcctc gccgggttcc ggcggttctt cgtcaacagc
     2341 atcgccgtga ccagcatctc gctgctgctc atcctggtcc ttggcgcgct cggagggtat
     2401 ccgctggcgc gcaaggagtt cccgggcaag gcggagttct ttggattcat tctgctcgtg
     2461 atcgccgtgc cgtgggtgat ctacctcatc ccgaccttcg tgatggagga tgccttggga
     2521 ctccgcaatt cctggcccgg gctcatcctt ccgtacgtcg ccctcaacct gccgtgggcg
     2581 ctcctgatca tgcgcagcgc gtttctcgcg atcccgcagg agatcgtcga cgcagcatcg
     2641 gtcgacgggg ccggcgagtt tcaaacgtgg ctccgggtgc tcgttcccct gaccgtgccg
     2701 ggactcacga cgacgttcct catcgtgttc gtctttatct ggaaggagtt cctctacgcc
     2761 gtcaccctga tgaccgagag cgagtggcag acgctccccg tcgggatcat ccagatccgg
     2821 gcggagctgc agagcctggt cttcaacatc ctcagcccca cgatcatcgt ggcgctcgtc
     2881 cccatcttcg tcgtcttcat cctgctgcgc aacttcttgg tggggggcgt cctggcgacc
     2941 ggtgggttcg gcaaggagta gccgtaagat cccggacccc cctcctgtgc gcaccgcccg
     3001 gccgagcgtg tgcttcggag gagattgggc ttcccatcag gcaaagtacg aggtgaggag
     3061 gatcagccaa tgcggttggg agtcacggtt gcgcatgccc gggtccacta cggcgatacc
     3121 ccgacgctcg accagtacgg ggcgtttgct gaatgggccg cgcgcatgcg gttcactggc
     3181 atcgaactgg ccgccttcac cgcggaacag ttcgtccgcg acttcggaga ggccggggct
     3241 ctgcggcagt tgggcgaacg ctgccatgcc ttgggcctca ccatcaacgc cttcgaggcg
     3301 gggttcctgc gccacatgac cgtcagcgaa gacgcggcgg tccgccgccg gggcgccgcg
     3361 gatctggacc gagccgtcca cgtcgcccga gcgctcggga gcgatctggt ctacttccac
     3421 tcggctccgc atccttcatg gaccatcgag ttcaagcggc tgtacgacga cttcagcccg
     3481 ccggtgaagg tcgacgttcc cgcagcgttc gcctgggacc aggcctggaa tcagtatgtg
     3541 gcgaccatca gcgaactggc gcgtatcgcc gagcgggccg gggtgcgcct ggcgatggag
     3601 atccggccat acgagatggt cagcaacgcc gacgggatgc accgcctgat cgatgccgtc
     3661 ggatctccgg ccctcggcgt ggtgttcgac accgcgcact ttctggttca gaaggaactt
     3721 ctgcccatcg ccattgagaa gttggggaac cgaatcttcc ttgtgcacct ggccgataac
     3781 gatggggcca ccgattatca ctgggcgccc ggcaaggggg cggtcccctg ggacggcgtc
     3841 cttcatgcgc tccggaagat acggtatgcg gggttcgcca acatcgacgt cgcggggacc
     3901 tacgacgaca tcgacgccga gatccgcgcg gggcgcgatt tcatcgcgaa tcgcttgggc
     3961 ctcgccgatc gttagcgcgc ggggcgcgag gcggcttcag tcggtcctga acaaggctcg
     4021 aacgaactgc acgccttggc gggccgcggc ctcgaagtct ggggcgagcg tcatctccaa
     4081 gccgatcgtc cggcggtagc cgatctcccg caacgttgcg gccaccgccg gaaagtccag
     4141 gtgcccccat cccggcgccc ggcggttgct gtcggcgaaa tgcacgtgcg ccaggtacgg
     4201 ggcggcctgc cggatcgcgc ccgggatcga tgcctcctcg atgttcatgt ggaacgtatc
     4261 gatcatcagg cgcacagacg ggagccccac cgcgccgacc aactcgagcc cctcggtcac
     4321 ggtgtggatg aagtcggtct cgtagcgatt gccgggctca accgccagga ccagacccag
     4381 atccccggcg cggcgcccgc actcgcggat gcagtcgatc aagtgccgca tcgcctccgc
     4441 ccgcgtcgtc ccgggctgca atttgccgcg aatgatcccg atggacacga ggggcgctcc
     4501 ggcgcgccgc gcggcgtcca tacactcccg catcgcctgc acggcgcggc gccgatcctc
     4561 gggattggga tgcgtgaacg tcaagccacg ttcgatggcg atgaatcccg acgcgatggc
     4621 ggcgaccccc agcttggccc gctccgccgt tgcgcgcagc ctggcgggat caggatcgtc
     4681 gacgtctcga ggcatgatct ccacggcgtc gaagccgagg gcggcggcct tggccacggc
     4741 gtcgtcgagg gcgagtcccg aagaagccgc ccagaagcgc gagccggtgg ccgcgagata
     4801 gagggaagtc ttcaccgcag aaaccgctca tcccgacgtg gcgcgcaacc gcatgccgga
     4861 ccggccgggc aggtgctcca cagacgccgt ttacaatgtt cgacccagat gcggaccaag
     4921 acgctctcca gcctggcgaa gtccatgccc cgaaggtcga tcccctccag cctgagcttg
     4981 agcctcctcg ccgcctctct ggcctcccgc gacaagggaa gtcgtatctt tgcgaccccg
     5041 cgcagctcgc ggaggaaaat ctcgaccgtc ttcggtccca cgcccttgat ctccatcaac
     5101 ctcgtctgga tgtgggggga ggacatctcc gcgacgatct gctgcaagcg ggcgcccggc
     5161 agggcgctgg caatggcaag cagtttggtg gccgtgctga aatcgtatcg aacgtaaccg
     5221 ccgcggtcga ggatcgtcac cagcttgtcc cacccttgtt tctggatcgc ctccggtgtc
     5281 gtgatcccct ccctaaacag gaggctggcg gtgtttcgcg ccacttccgc ccggatgggc
     5341 tttgcaaaca gcgccgcggc gacaaaccac gggaaccagt gccgcttgtc tcggagatcc
     5401 aggccgacgg cctcggcgta tgtcttctgg cgcaataagg ctttgccgag gagcccgcgg
     5461 ccgaaggccc tgtgccgggc agccgtcacg ggcgcgagtg ccgcggcgtg gaatccgacc
     5521 ccaacgatgc accggccgtc aacacgagga gcgttctcga ggcgtgtgtt gatatcc
//
LOCUS       DASQGH010000401         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4630, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000401 DASQGH010000000
VERSION     DASQGH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4630"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..457)
                     /locus_tag="VEW91_07885"
     CDS             complement(17..457)
                     /locus_tag="VEW91_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYM91534.1"
                     /translation="MPVPAPGDAVPPLAKEAITKVQLLKYAGASGDHNLIHTDLETAR
                     AVGLPGIIAHGMLSMGFLGQFLVGLAGPEGVRRLRVRFNAIVRPGDVLTCRGTVTSVQ
                     EPSGGKRVVALEVWAENQQGERVTTGEAEVYWPDPEAGRGPWDV"
     gene            complement(475..873)
                     /locus_tag="VEW91_07890"
     CDS             complement(475..873)
                     /locus_tag="VEW91_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HYM91535.1"
                     /translation="MAIDRALIGTRSEPHTFEVERGAIRKFAEAIGDAHPAFRAGEVA
                     PPTFPTTFSMAVPLLKTDPARVLHGEEEFVYERPLRAGDRVTCIRRIVDVYERRGRRL
                     GPLACVVIETEGRDPQGTLLYRGRSTLLVR"
     gene            complement(936..1520)
                     /locus_tag="VEW91_07895"
     CDS             complement(936..1520)
                     /locus_tag="VEW91_07895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HYM91536.1"
                     /translation="MTTDPLRAFLDGLAEFGREHDAHETEHARRLLNAGPEAGQLLWI
                     LVRATRARRILEIGTSNGYSTIWLAWAAGGTGGHVITIDRAAAKVAMARENLQRAGLV
                     DRVTLLEGAALAILAGLDGPFEFVFLDADRPNYLAYVDPLLSLVPPGGLLATDNVLSH
                     ARELQGYLHRLTRDPGLVSVTLPVGNGVLLTYKR"
BASE COUNT          269 a          595 c          525 g          235 t
ORIGIN      
        1 cccaggaatc gcacggtcac acgtcccacg gtcccctccc cgcctccggg tccggccaat
       61 acacctcggc ctcgcccgtc gtcacgcgtt cgccctgctg gttctcagcc cacacctcga
      121 gcgcgaccac gcgtttcccg cccgagggct cctgcacaga cgtgacggtg ccgcggcagg
      181 tcagcacgtc gcccggccgg acgatggcgt tgaaccggac cctcaggcgc cgcacccctt
      241 ccggccccgc cagcccaacc aggaactggc cgaggaagcc catcgagagc atgccgtgcg
      301 caatgatccc gggcaggccc acggcgcggg cggtctccag gtcggtgtga atcaggttgt
      361 ggtcccccga ggcccccgcg tacttcaaga gctggacctt cgtgatggcc tctttggcga
      421 gcggcggcac ggcatccccg ggggcgggta caggcatcac cacacgggac gctctcagcg
      481 cacgaggagc gtgctgcggc cgcggtacag cagggtgccc tggggatccc ggccctcggt
      541 ttcgatgacc acgcaggcca gcggaccgag gcggcgcccc cggcgctcat acacgtccac
      601 gatgcgccgg atgcacgtga cccggtcccc ggcgcggagg ggccgttcat acacgaactc
      661 ctcctcgccg tgcaagacgc gggcggggtc ggtcttcagc agcggcaccg ccatcgaaaa
      721 cgtggtgggg aacgtgggag gtgccacctc cccggcccga aacgcggggt gggcatcgcc
      781 aatcgcctcg gcaaactttc tgatcgcccc gcgctccacc tcgaaggtgt gaggctcgga
      841 cctcgttccg attagcgcac gatcgatcgc cacggtcgtt cccacctgcc cgcccactct
      901 acagcttgga ggtggggaag cgtttctcct tgagatcatc gcttgtaggt cagcaggacg
      961 ccattgccaa cgggcagcgt gacgctgacc aacccgggat cccgcgtcag ccggtgcaga
     1021 tacccctgca gctcgcgcgc atgcgagagc acgttgtcgg tggcaagcag cccccccggc
     1081 gggacgagcg atagcaaggg gtcgacgtag gcaaggtagt tcggccggtc cgcgtcgaga
     1141 aagacgaact cgaacgggcc gtccaggccg gccaggatcg cgagcgccgc cccctccagg
     1201 agcgtcaccc ggtccaccaa tccggcgcgc tggaggtttt cgcgggccat cgcgaccttg
     1261 gccgcagccc ggtcgatggt gatcacatgg cctccggttc caccggctgc ccaagcgagc
     1321 cagatcgtcg aatacccatt ggaggtgccg atctcaagga tccggcgcgc ccgcgtcgcg
     1381 cgcaccagga tccacaggag ctggccggcc tccgggccgg cgttcaggag ccgccgtgca
     1441 tgctcggttt catgcgcatc atgctcccgc ccgaactcgg ccaagccatc caggaacgcc
     1501 ctgagcggat ctgtggtcat gcgcgatcgc tccttcgtga ccggggctcg gccaccagca
     1561 cggcgaatcc ggaaagccca agcgcggcgg caaacgccca gaacacccgc tcgtatccgc
     1621 cgag
//
LOCUS       DASQGH010000402         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30174, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000402 DASQGH010000000
VERSION     DASQGH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30174"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1124
                     /locus_tag="VEW91_07900"
     CDS             240..1124
                     /locus_tag="VEW91_07900"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HYM91537.1"
                     /translation="MAAVLALLPGTLVGAAPGASSPAPLPKGWGWLLASGLVAGVDPS
                     GRTMTLAIAGQGRLETFEGQSKWRSQPAAGTQVVHLVPATVISDTGGEPATISAIRTG
                     APAMVWGAVRPDASVFGLKVVVAPPSTRPSPALSAAAPYALSGAVLRRAGGMLELLTR
                     QGTRRSVLVTGATTVRGVTGGVVPASAIGPYDVLKVEGTVNSDGSIAATYIDLDVDAA
                     TAPQVSGVVEQVFGEVEGLVVGGVMVPVPPGCYFIKGMGPGAFRQLAPGQPVSVYGTS
                     ISAGGMPIGLHARVVVAR"
     gene            1131..1430
                     /locus_tag="VEW91_07905"
     CDS             1131..1430
                     /locus_tag="VEW91_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HYM91538.1"
                     /translation="MDLGALQARIAATFSARDRARGLDGTFRRLVEEIGELAKALRGA
                     DPQAVALELSDVVAWTVSVAVLCGVDLDWALSRYGAGCPRCGASPCRCPMDTSAR"
     gene            complement(1437..2261)
                     /gene="speB"
                     /locus_tag="VEW91_07910"
     CDS             complement(1437..2261)
                     /gene="speB"
                     /locus_tag="VEW91_07910"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYM91539.1"
                     /translation="MTFLASRPPGAGPGPGPTLIGVPYDATSSFRRGSRWGPAAIRAA
                     SESIETYNPLIDRDLEQVPFVDGGDLQVEGLDPERMVCAVRRHLGPGVPFLLGGEHTI
                     TLGAVQALVARYHDLVVIQWDAHTDLRAEYQGNPVCHATTMRRLLDGGCPLVQLGIRA
                     GTREEFAIGRARSLYLSRGALLPDPLLDTLRSRPVYLSVDIDVLDPSVAPGTGNPEPD
                     GATYAELLAGLRSLTRHRVVGMDLVEVAPPWDPGGRTAVIAASLVRDMLLLFTPAP"
     gene            complement(2258..>2956)
                     /gene="speE"
                     /locus_tag="VEW91_07915"
     CDS             complement(2258..>2956)
                     /gene="speE"
                     /locus_tag="VEW91_07915"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HYM91540.1"
                     /translation="AVQTTEGDEFAYHEMLAHPPLCTHPAPRRILIIGGGDGGLLEEV
                     LKHPVERVTMVEIDEAVVRAGRQYLGTICGRAFEDPRATLVIGDGIAYVRETMDRFDV
                     VLVDSTDPQGAATGLFAQEFYAQVAHHLTPDGLMAVQSGSAVYQQDLIRTVRRHLRPA
                     FPLVRTYLASVAAYPGGLWSFTMGSLGCDPLLVGPDAIAPRIGEFRLRYYTPSGHHAA
                     FDLPPHIRGEVEAG"
BASE COUNT          412 a         1014 c         1096 g          434 t
ORIGIN      
        1 ggggaccgac ctccccacca ccgtccggtt cgtccggggg agcctaaatg caaacgacct
       61 ggccgggcaa ctgggaatgc gaccggaagc cctgcagcgg accgtgcagc gggcgatcgg
      121 cgatgcctcc ggagtgcccg cgctctcctc cacccgctag cccctggaac atttccggcc
      181 actgtcacgt tcctgtatca agaatgggat gcgcagcctc tcggccaatc gtcccgatcg
      241 tggcggcggt cctcgcgctg ctcccgggga ccctggtcgg tgccgccccg ggagcgagtt
      301 ctccggctcc gctgcccaaa gggtgggggt ggctcctggc ttcgggcctc gtggcggggg
      361 tggatccgag tgggcggacc atgaccttgg cgatcgccgg acagggccgc cttgagacgt
      421 tcgagggtca atcgaagtgg cgcagccagc cggcggccgg cacacaggtc gtgcacctgg
      481 ttcctgcgac cgtcatctcg gacaccggcg gtgaacccgc gacgatctcc gcgatccgga
      541 ccggcgctcc ggcgatggtg tggggtgcgg tgcggccgga tgcctcggtg ttcgggttga
      601 aagtggtggt ggcccccccc tccacccgac cctcgccggc cttgtcggcg gccgcgccct
      661 acgcgctttc gggggcggtg ctccgacggg ccgggggcat gcttgagctg ttgacccgcc
      721 aggggacccg ccgcagcgtg ctcgtcaccg gggcgacgac ggtccggggc gtgaccgggg
      781 gcgtggtccc ggcctcggcg atcgggccgt acgatgtgct caaggtagaa gggaccgtga
      841 actcggacgg gagcatcgcg gcgacctaca ttgacctgga tgtggacgca gccaccgccc
      901 cgcaggtatc cggggtggtg gagcaggtgt ttggagaggt ggaaggactc gtggtgggcg
      961 gcgtgatggt ccccgtcccg ccgggatgct atttcatcaa gggcatgggg ccgggcgcgt
     1021 tccggcaact cgcccccggg cagcccgtgt ccgtctacgg cacgtcgatc tctgccggcg
     1081 gcatgccgat cggcttgcac gcgcgcgtcg tcgtcgcgcg ctaggatccc atggacctcg
     1141 gtgcgctgca ggcccggatc gccgccacct tcagcgcgcg ggatcgggcg cggggcctgg
     1201 acggcacgtt ccggcggctg gtggaggaaa tcggcgagct ggcgaaggcg ttgcgtggcg
     1261 cggaccccca agccgtcgcg ctcgagctgt cggacgtggt cgcctggacc gtgagtgtgg
     1321 cggtgctgtg cggcgtggat ctcgactggg cgctgagccg gtacggcgct ggatgtcccc
     1381 ggtgcggcgc gtccccgtgc cggtgcccga tggacacgag cgcccggtaa acggcgctac
     1441 ggcgccggag tgaagaggag cagcatatcc cgcaccaggg acgccgcgat caccgcggtc
     1501 cgtcctccgg gatcccacgg cggggctacc tccacgaggt ccatgccgac gacgcggtgg
     1561 cgggtgaggc tccggagtcc ggccagcagc tctgcgtagg tggccccgtc cggctcgggg
     1621 tttcccgtcc ccggcgccac cgatgggtcg agcacgtcga tatcgacgct gagatacacc
     1681 ggccgggatc tcagggtgtc gaggagcggg tccgggagca gggcgccccg cgagaggtac
     1741 aggctccgcg cgcggccgat cgcgaactcc tcccgggtcc ccgcgcggat ccccaactgg
     1801 acgagcggac atccgccatc caggaggcgg cgcatcgtgg tggcgtggca caccgggttg
     1861 ccctggtact cggcccgcag gtccgtgtgg gcgtcccact ggatgaccac gaggtcgtgg
     1921 taccgagcga cgagcgcctg caccgcgccc agcgtgatcg tgtgctcgcc cccgagcagg
     1981 aagggcaccc cgggcccgag atggcgccgg accgcgcaga ccatgcgctc gggatcgagc
     2041 ccttcgacct gcagatcgcc cccgtccaca aagggcacct gctccaggtc ccggtcgatg
     2101 agcgggttgt aggtctcgat gctctccgac gccgcccgga tcgccgccgg gccccaccgg
     2161 ctgccccggc gaaacgacga ggtcgcgtcg tacgggacgc cgatgagggt ggggccaggg
     2221 ccgggtcccg cccccggggg ccgcgacgcg aggaaggtca ccccgcctcc acctcacccc
     2281 ggatgtgcgg ggggagatcg aacgccgcgt ggtgtccgga cggggtgtag tacctgagcc
     2341 ggaactcccc gatccggggg gcgatcgcat cgggccccac cagcagcggg tcgcacccca
     2401 ggctccccat cgtgaacgac cagaggccgc ccggataggc tgccacgctc gccaggtacg
     2461 tgcggacgag cggaaacgcc gggcgaaggt gccgccgcac cgtccggatc aggtcctgct
     2521 ggtagacggc ggacccggac tggacggcca tcagtccgtc cggggtgagg tggtgggcga
     2581 cctgcgcata gaactcctgg gcgaagagcc cggtcgccgc cccctgggga tcggtcgagt
     2641 cgaccagcac gacgtcgaac cgatccatgg tttctcggac gtaggcgatg ccatcgccga
     2701 tgaccagcgt ggcccgggga tcttcgaacg cgcggccgca gatcgtgccg aggtactggc
     2761 ggcccgcccg gaccaccgcc tcgtcgatct cgaccatggt gacccgctcc accggatgct
     2821 tgaggacttc ctcgaggagc cctccgtccc cgcccccgat gatgaggatc cggcggggcg
     2881 ccgggtgcgt gcagagggga gggtgggcca gcatctcgtg gtacgcgaac tcatcgccct
     2941 cggtggtttg caccgc
//
LOCUS       DASQGH010000403         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10156, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000403 DASQGH010000000
VERSION     DASQGH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10156"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VEW91_07920"
     CDS             <1..1192
                     /locus_tag="VEW91_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYM91541.1"
                     /translation="TKAELRREQAEYPPFGRYLCIDRSEVARIHGTSGTTGKPTAFAV
                     GKDDWPRIANAHARIMWGMGLRPGDTIFLASFFSLYLGSWGALAGGERLRATCFPFGA
                     GVPGQTRLAVRWLLDTRPTAFYGTPSYALHLAQVAASEGVDARDFGLRVMVFSGEPGA
                     GIPATKRQIEETFGARCFDSGSMAEMTPWMTNAECEFRHGMHLWQDIVYCEVCDPRTF
                     VPVAYGREGTPVYTHLERTSQPMIRLVSGDLTRWVDDPCECGRTYPRLPGGILGRLDD
                     MCIVRGANIYPSAVEDVLRTIEGFGGEFQMVISRERAMDELVIRVEYTQDVARHAAMA
                     PGTLEALRERIGAQLRSVIGIRPVVRLEPPGTLPQTEFKARRVVDNRGLYEESVRSTG
                     GGSR"
     gene            1189..2136
                     /gene="meaB"
                     /locus_tag="VEW91_07925"
     CDS             1189..2136
                     /gene="meaB"
                     /locus_tag="VEW91_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HYM91542.1"
                     /translation="MTELAAFEVGNRRRLGRLLTAVENDPAAAAKIVREVWARTGGAH
                     RIGITGPPGVGKSTLVDHLIGMARADGQTVAVVAVDPSSPFSGGAILGDRVRMLRHSS
                     DPGVYIRSMAARDHLGGLAAATRDAAHVLDAFGFGVLLLETVGVGQSELDIMRAVDTV
                     VVVTAPGLGDSIQTLKAGILEIADLLVVNKADRPGAKETALQLREMRHLAPRPSGWDV
                     PILQTVASEGTGIAELWGAIRQHREYLRASAEGATRRGREAEREVMDLVERALAARLH
                     ATLGEPGHLGEILQQAKDRTLDPHSAAKAILDRFFTRSS"
     gene            2180..3106
                     /locus_tag="VEW91_07930"
     CDS             2180..3106
                     /locus_tag="VEW91_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM91543.1"
                     /translation="MSGAPGVTVVDLWERGRRYCTSGYLIAAPRPALVDAGAARSVPV
                     WLDTLAYLGVPPADVAYIIVTHIHLDHAGGVGILLRHLSNAQVVVHQHGARHLVDPSR
                     LVASARGVFGEHLETYFGLPEPVPEERLLVPGPAGELDLGGGHRLRFFDAFGHARHQH
                     VILDAGAGCLFGGDEFGKQFVTIASDYLLPDTPPTQFDPVAWTRSVDLLLTLRPQAVF
                     LAHFGRCALSCDALGQRLREQVEAFAALGRAGPQALPWEEVRARLTAHVRRDLAARGV
                     AWTATIEHETAEELEIWAKGIADYQARRSRPG"
     gene            complement(3120..>3554)
                     /locus_tag="VEW91_07935"
     CDS             complement(3120..>3554)
                     /locus_tag="VEW91_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYM91544.1"
                     /translation="SVLAAYYLPEPQTRAAQDFLRRTPEAAVSDLVEVEFVSALARRV
                     RIGDLPRQDAQRISALFISHIEGQVYARLSVEQEAYRTARTWLTGFEVPLRTLDALHL
                     ATAAAHGVPIATADRALARAADALGIRVRRIGRPAAGATHAM"
BASE COUNT          506 a         1243 c         1296 g          509 t
ORIGIN      
        1 gaccaaggcc gagctacgcc gcgagcaggc cgagtacccg ccgtttggcc gctacctgtg
       61 catcgatcgc tcggaggtcg cccggatcca cggaacatcg ggcaccaccg gcaagcccac
      121 ggccttcgcc gtcgggaagg acgactggcc gcgcatcgcc aacgcccacg cgcggatcat
      181 gtgggggatg ggcctccgcc ccggcgacac catcttcctc gcatcgttct tcagcctcta
      241 cctcggcagc tggggggcgc tcgcgggcgg ggagcggctg cgcgccacct gcttcccgtt
      301 cggggcgggg gtcccgggcc agacgcgcct ggcggtgcgc tggctcctcg acacgcgccc
      361 caccgcgttc tacggcacac cgtcgtacgc cctgcacctc gcgcaggtgg cggccagcga
      421 gggtgtcgac gcgcgcgatt tcgggctccg ggtgatggtc ttttccggcg agccgggcgc
      481 gggcatcccg gccaccaagc gacagatcga ggagacgttc ggggcgaggt gcttcgacag
      541 cggcagcatg gccgagatga cgccctggat gaccaacgcg gagtgcgagt tccggcacgg
      601 catgcacctg tggcaggata tcgtgtactg cgaggtctgc gacccgcgca cctttgtgcc
      661 ggtggcctac ggccgggagg ggacgccggt ctacacgcat ctcgagcgca cgtcccagcc
      721 gatgatccgg ctggtgtcgg gcgacctcac ccgctgggtg gacgacccgt gcgagtgcgg
      781 gcggacgtat ccccgcctgc ccgggggcat cctcgggcgc ctggacgaca tgtgcatcgt
      841 ccggggcgcc aacatctacc ccagtgcggt ggaggacgtc ctgcgcacga tcgagggatt
      901 cggcggcgag ttccagatgg tcatcagccg ggagcgggcc atggacgagc tggtgatccg
      961 ggtggagtac acccaggacg tggcccgcca cgccgcgatg gcccccggca ccctcgaggc
     1021 cctgcgggag cggatcgggg cgcagctgcg gtcggtcatc gggatccgac cggtcgtccg
     1081 gcttgagcca cccggcacgc ttccccagac ggagttcaag gcccgccggg tcgtggacaa
     1141 ccgcggcttg tacgaggaga gcgtgcggtc gacgggcggc gggtcgcggt gaccgaactg
     1201 gcggccttcg aggtcgggaa ccggcgccgc ctcggccgat tgctgacggc ggtcgagaac
     1261 gatcctgccg ccgccgcgaa gatcgtgcgc gaggtgtggg cgcgcaccgg cggggcccac
     1321 cgcatcggca tcaccgggcc gccgggcgtg ggcaagagca ccctggtcga ccacctgatc
     1381 gggatggctc gggccgacgg ccagaccgtg gccgtggtgg cggtggaccc ctccagtccc
     1441 ttcagcggcg gggcgatcct gggcgaccgc gtccgcatgc tccggcacag cagcgacccc
     1501 ggcgtgtaca tccggagcat ggcggcgcgc gaccacctcg ggggccttgc ggcggccacc
     1561 cgggatgccg cgcacgtgct cgacgccttc gggttcggcg tcctcctgct ggaaacggtc
     1621 ggggtcgggc aaagcgagct ggacatcatg cgcgcggtcg acaccgtcgt ggtcgtcacg
     1681 gcgccggggc tgggagactc catccaaacc ctcaaggccg ggatcctgga aatcgccgat
     1741 ctgctggtgg tcaacaaggc cgaccgtccg ggcgccaaag aaaccgccct ccagctgcgg
     1801 gagatgcggc acctcgcacc caggccgtcg ggctgggacg tccccatcct ccagaccgtc
     1861 gcgagcgagg ggacggggat cgcggagctc tggggggcga tccggcagca ccgggagtat
     1921 ctgcgtgcgt ccgccgaggg tgcgacgcgc cgcggccggg aggccgaacg cgaggtcatg
     1981 gacctcgttg agcgagccct ggccgcccgc ctgcacgcca cgctggggga gcccggtcat
     2041 ctcggcgaga ttctgcagca ggcgaaggac cggacgctcg atccccactc ggccgcgaaa
     2101 gcgatcctcg accggttctt cacgcgttca tcgtagcatg agcacgaggg gcgacggatc
     2161 gctcgaaggc ggggccgtca tgagcggagc ccccggcgtc accgtcgtcg acctctggga
     2221 gcggggccgg cgctactgca cctcggggta cctgatcgct gccccgcgtc ccgcgctcgt
     2281 ggacgccggc gccgcccgca gcgtgcccgt ctggctggac acgctggcct acctgggcgt
     2341 cccgccggcc gacgtcgcgt acatcatcgt cacgcacatt cacctggacc acgccggggg
     2401 tgtggggatc ctgctgcggc acctcagcaa cgcccaggtg gtggtgcatc agcacggggc
     2461 ccgccacctg gtcgatccga gccggctcgt cgccagcgcg cgaggggtct tcggcgagca
     2521 cctggagacg tacttcggcc tcccggaacc cgtcccggag gagcgcctcc tggtgccggg
     2581 gccggcgggc gagctggacc tgggaggggg ccaccggctg cggttcttcg atgcctttgg
     2641 ccacgcccgg caccagcatg tgatcctgga cgcgggcgcg ggctgcctgt ttggcgggga
     2701 cgagttcggg aaacagttcg tgacgatcgc gagcgactat ctcctaccgg acacgccgcc
     2761 cacccagttc gatcctgtag cctggacccg ctcggtcgat ctgctgctga ccctacggcc
     2821 gcaggccgtg ttcctggccc acttcggccg ctgcgcgctc agctgcgacg ccctcggcca
     2881 acgcctccgc gagcaggtcg aggcgttcgc cgccctgggg cgcgccggtc cccaggcgct
     2941 gccgtgggag gaggtgcgtg cgcgcctgac cgcgcacgtg cggcgcgacc tggcggcccg
     3001 gggcgtggcg tggacggcga ccatcgagca cgagacggcc gaagaactgg agatctgggc
     3061 gaagggcatc gccgattatc aggcgcgccg ttcgcgcccg gggtgacggg gcaggcgtcc
     3121 tacatcgcgt gggtggcgcc ggcggccgga cgaccgattc ggcggacgcg aatcccgagt
     3181 gcatctgctg ctcgggcgag cgcacggtct gccgtcgcga tcggcacgcc gtgggcggcc
     3241 gccgtggcca ggtgcaacgc gtcgagcgtc ctcaacggaa cctcgaaccc cgtcagccac
     3301 gtcctggctg tgcgatacgc ctcctgctcc acagaaagcc gcgcgtacac ctgaccctct
     3361 atgtgggaga tgaacagcgc agagatgcgc tgcgcgtcct gccgcgggag gtcgcctatc
     3421 cgcacccggc gcgccagcgc cgagacgaac tccacttcaa caaggtcgct cacggctgcc
     3481 tccggggtcc tgcgcaagaa atcttgggct gcccgcgttt gtggctcggg aagatagtat
     3541 gcggcgagca cgct
//
LOCUS       DASQGH010000404         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52712, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000404 DASQGH010000000
VERSION     DASQGH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52712"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEW91_07940"
     CDS             complement(<1..194)
                     /locus_tag="VEW91_07940"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HYM91545.1"
                     /translation="MTETIVAMAVATFLTRAAGVMGAPVRDPWAVRAITLVPVAAFAA
                     LVSSSLEVRADAWLRWLVVG"
     gene            complement(187..879)
                     /locus_tag="VEW91_07945"
     CDS             complement(187..879)
                     /locus_tag="VEW91_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HYM91546.1"
                     /translation="MPREADPPRSRFVTGVVEALPLWLGVAPFGFVYAVAGRRVGLTG
                     WEAQLMSLLVYAGGAQFTAVRLMAQGAPKHLLVVATFLINLRHLLYGVSVAPYLRAVR
                     PRLRGAAAFFLVDESYGLAMRAYLEGRGSLQHLVGAGASLYLAWNLGTALGTVFTGAF
                     PDPRGLGLDLVFPLTFVALVAPLLREGANRRAALVSFLAGIAVIPVLPGGVGFIAATL
                     LGLLGGLVGSHD"
     gene            1125..1658
                     /locus_tag="VEW91_07950"
     CDS             1125..1658
                     /locus_tag="VEW91_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYM91547.1"
                     /translation="MKGRWLSYLPMVLAVCVVAAAAGYLVTHPPGSAPPAPPPAGTGP
                     TVAPGPTPAPPPTTEPAPATTPEPPAPPPAAGPFTQPPASPTPVPPASAPAAPPAPRG
                     PAGAQRFHVQAGAFTRLENAEDLVRKLRAKHYSASIVKGQYYRVWIGQPLDRPGAEQL
                     AERLRADGFETALIPGP"
BASE COUNT          286 a          630 c          575 g          191 t
ORIGIN      
        1 atccccacca cgagccaccg cagccaggca tcggcccgga cctccaggct cgaagacacc
       61 agggcggcaa acgccgcgac aggaaccagc gtgatcgcgc gcaccgccca cgggtctcgg
      121 accggcgcgc ccatcactcc ggcggcgcgc gtgagaaacg tcgcgacggc catcgccaca
      181 atcgtctcag tcatgcgagc ccacgagtcc ccccagcaat cccagcagcg tggcggcgat
      241 gaacccgacg ccgcccggca gcaccggaat gaccgcgatc ccggccagaa aggataccag
      301 cgcggcccgg cggttcgcac cctcgcgcag cagcggcgcc accagcgcga cgaacgtcag
      361 cggaaacacc agatccagcc cgagaccccg cggatcgggg aacgccccgg tgaataccgt
      421 gccgagggcc gtcccgaggt tccaggccag gtagagggag gccccggcgc ccaccaggtg
      481 ctggaggctg ccgcgcccct cgaggtacgc ccgcatggcg aggccgtaac tctcgtccac
      541 gagaaaaaac gccgccgccc ctcggagccg gggacggacc gcgcggaggt acggggcgac
      601 cgagacgccg taaagcaggt gccggaggtt gatcaggaag gttgcgacga ccagcaggtg
      661 cttcggcgcc ccctgggcca tcagccggac ggccgtgaac tgggccccgc cggcgtacac
      721 caagagggac atgagttgtg cttcccagcc ggtgagcccc accctccgcc ccgccacggc
      781 gtagacgaac ccaaacgggg cgacgccgag ccacagcggt aaggcctcca ccacgccggt
      841 cacaaaccgg ctccgcggcg ggtcggcttc tcgcggcact ctcctgccgc ctcagcccgg
      901 gggcagcccg tacgggtggc gcaagtcggt cgggggcagt tggatgatga tgacgacggc
      961 gagcattgcc gaggctcctt cagggcgggt tccgcgcccc ccaccccgtg tcctcctcgg
     1021 ggatgcccgt tggcgggtgg gagcaggtaa ttgatccggg agaaggaatt gacaccggga
     1081 gacgtgcagg gatcgtcgca ggtcgggaag gagcgacgga agcggtgaag gggagatggc
     1141 tttcctacct gccgatggtg ctggcggtgt gtgtggtcgc cgctgcggcc ggctacctgg
     1201 tcacgcaccc gccgggatcg gcgccgcccg cgccgcctcc ggccgggacg ggaccgactg
     1261 tggccccggg acccaccccg gcgccgcccc cgaccactga gccggcgccc gccacaacgc
     1321 cggagccacc tgcgccgccc cctgcggcgg gcccgttcac acagccgccc gcttcaccaa
     1381 ccccggtccc acccgcgagc gcgccggctg caccacccgc gccgcgtggt cccgcggggg
     1441 ctcaacggtt ccacgtccag gccggcgcat tcacccgcct cgagaacgcc gaggatctgg
     1501 tcaggaagct ccgcgccaag cactactctg caagcatcgt caaggggcag tactataggg
     1561 tctggatcgg ccagcccctc gaccggcccg gagccgagca gttggccgag cgccttcgag
     1621 cggatgggtt cgagaccgcg ctgatccccg gcccgtgacc tcttcggaat ccggggccgg
     1681 cg
//
LOCUS       DASQGH010000405         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21474, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000405 DASQGH010000000
VERSION     DASQGH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21474"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VEW91_07955"
     CDS             complement(<1..484)
                     /locus_tag="VEW91_07955"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HYM91548.1"
                     /translation="MTFQDILIALERYWAAYGCVIAQPYDVEVGAGTMHPATFLRALG
                     PEPWKAAYLQPSRRPTDGRYGQNPNRLYQHLQYQVILKPAPSDVQDLYVRSLRALGIE
                     LRDHDLRFLEDDWEAPTLGAWGLGWQVFLDGQEISQFRYFQQVGGMDVPVVSAELPYG
                     L"
     gene            complement(549..791)
                     /locus_tag="VEW91_07960"
     CDS             complement(549..791)
                     /locus_tag="VEW91_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91549.1"
                     /translation="MEIDLERAKRAIAQFGSAIDEIRRAVRPGRSGMVRIHTEDRVLE
                     LPLTWAAAGFAALAVATVLTSVPLRREREEEPLGIG"
     gene            1111..>1622
                     /locus_tag="VEW91_07965"
     CDS             1111..>1622
                     /locus_tag="VEW91_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYM91550.1"
                     /translation="MPRPKPGLFDPAVEDLQGTTRQIVELTLRIARAMGMREDEINNV
                     RLGAFLHDIGMAGIPDDVLFKAGPLSKQEQEIVRQHPAYAHELLSRAPAFAAALDIPY
                     CHHERWDGTGYPHGLKGREIPLAARIFAVVDAWFALRSERPYRPAWTAEDAHVYIVQR
                     AGRDFDPAVV"
BASE COUNT          286 a          546 c          526 g          264 t
ORIGIN      
        1 cgagcccgta cgggagctcg gccgagacga ccgggacatc catcccgccc acctgctgga
       61 agtacctgaa ctggctgatc tcctggccgt cgaggaacac ctgccagccg agcccccagg
      121 ccccgagggt cggggcttcc cagtcgtcct ccaggaaccg gagatcatgg tcgcgcagtt
      181 cgatccccag cgcgcgaagg ctgcgcacgt acaggtcctg gacgtcgctg ggggcgggtt
      241 tcaagatcac ctggtactgc agatgctggt agaggcggtt gggattctgg ccgtaccggc
      301 catcggtcgg ccgccggctc ggctgcaagt acgccgcctt ccacggctcg ggacccagcg
      361 cgcggaggaa cgtggccggg tgcatcgtcc cggccccgac ctcaacatcg tacggctgcg
      421 cgatcacgca gccgtacgcg gcccaatacc gttcaagcgc gatgaggata tcctgaaatg
      481 tcatccgtcc ctcctcccac actctcctgc agttcgggcg aacggatgac cgatcctgtc
      541 cgggccggct atccgatgcc gagcggttct tcctcgcgct cccgccgcag gggaacagac
      601 gtcaggaccg tcgccacagc cagggcggca aaccccgccg cggcccaggt caggggcagc
      661 tcgagcacgc ggtcctcggt gtggatccgc accatgccgc tgcgccccgg ccgtaccgcg
      721 cgccggatct catcaatcgc cgacccgaac tgggcgatcg cgcgcttcgc ccgctccaga
      781 tcgatctcca tcggaccacc tccgcgggtc tatcttatca cgcggagcag ccgcgtgggg
      841 gtgaacacga cctgcgaatg gaacctccgc agggcgatcg ctgacggcgc gccatgccgc
      901 cggtaggcgc agtggcccac caagacgcag acgaaaagcg gcccctgacg cgggatccca
      961 atgctcgggg caagtggtct tggacgtccg cccggagggc atcagtagca gggattgagg
     1021 cgttgggagg acaccatgtc tctacgattt cgaggccgac atcgcaacac cggatccatc
     1081 ctggacaagg tccaggactt tctgaacccc gtgccgcggc ccaagccggg gctcttcgat
     1141 cctgccgtag aggaccttca gggtacgact cggcagatcg ttgaactgac gttgcggatc
     1201 gcccgcgcca tgggcatgcg ggaggacgag atcaacaacg tccgactcgg ggcattcctg
     1261 cacgatatcg gcatggccgg tatcccagac gacgtgctct tcaaggcggg gccattgtcg
     1321 aagcaggagc aggagatcgt ccggcagcat ccagcgtacg cgcacgagct gctgtcccga
     1381 gcgccagcat tcgccgcggc cctggatatc ccatattgcc atcatgagcg gtgggacgga
     1441 acgggatacc cccacggcct caaaggtcgg gagatcccgt tggctgcgcg gatcttcgcg
     1501 gtggtggacg catggtttgc gctgcggtca gagcgaccct accggcccgc gtggacagcg
     1561 gaggacgctc atgtctacat tgtgcaacgt gctggccgcg attttgatcc tgcggtggtc
     1621 ga
//
LOCUS       DASQGH010000406         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49863, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000406 DASQGH010000000
VERSION     DASQGH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49863"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            646..>1635
                     /locus_tag="VEW91_07970"
     CDS             646..>1635
                     /locus_tag="VEW91_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HYM91551.1"
                     /translation="MLLLVACALSIAIPLYRSGQPSPAVLPYTEFLDRVEHGEITVVT
                     LRDGTATGALPNHIPFLTYLPDETSHYLRLLEEKGVTIRVVPQPRHALSLWLILMLPV
                     VFFLLMWIVYARQPSGPPVLRFGKSRARIYVAAHTGVTFNDVAGVDEAKEELEEIIEF
                     LRHPQKFQALGAKIPRGVLLLGPPGSGKTLLARAIAGEARVPFFSISGSEFVEMFVGV
                     GAARVRDLFDQAKKSTPCLVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFD
                     PNSGIIVIAATNRPDILDPALLRPGRFDRRIVVDNPDTKGRRAILDVHVRGKPL"
BASE COUNT          305 a          511 c          525 g          294 t
ORIGIN      
        1 ggccgtcgct tcgcttaccg aaatcgtaat gaatgccagc gtgcgagacc atccggtccg
       61 gggcgtattc tcttgatccg taagcgacaa cgtcggtcgc ggtcaagcgc gccgcagaac
      121 tcaagaagcc gtcactcccc gcattcgcaa tacactcttc acccgataga cgggctagag
      181 acgatcgaat tatagtgagc ggtgggagtg caggcccaag cacaaagcag ccgaagaagc
      241 ccgtctacgt ggaagctgag cgtcggtaag tgtacttcgg aggtaactcc ggagaacggc
      301 tggtcgacgg taggctttcg gcggggcggc cgaagacggt gaaaagccga acaaaagcct
      361 gcgctcccgt ttctatcttg gctcgccaga cccatccgaa cgctcacacg gcgacgatcg
      421 ccttccctac ccggcagccg gatttccctg cttccatcat cacccggcgc gagtcgcggg
      481 ggtagcacat gttggcgcag gtcagcccgg gcgccgccta aagtactcag ccggactgat
      541 agtcggagcg aaactactat attactctca aagggggcga aggcgagaga ggaggttaca
      601 cgccacgtgg cgccggactc cgccccaagg gtagatcttt ggtttatttt actgcttgtc
      661 gcttgtgcgc tgagcatagc tatcccattg taccggtctg gccagccttc accagccgtc
      721 ctcccctaca ccgaatttct ggatcgtgtt gaacacgggg agatcaccgt cgtcaccttg
      781 cgcgacggca ccgccaccgg cgccttgccc aatcacatcc ccttcctcac ctacctcccc
      841 gacgaaacct cccactacct gagactgctt gaggagaagg gcgtcacgat cagggtggtc
      901 ccacagccac ggcatgctct atcgttgtgg ctcatcctca tgctgcccgt cgtgttcttc
      961 ctgctgatgt ggatcgttta cgctcggcag ccgagtggtc cgccggtact cagattcggc
     1021 aagagccggg cacggatcta tgtcgccgcg catacgggcg tgaccttcaa cgacgtggcg
     1081 ggggtggatg aagccaagga ggaactcgag gagatcatcg agttcctccg gcacccgcag
     1141 aagttccagg cgctcggggc gaagatcccg cgcggcgtgc tcctgttggg gccgccgggc
     1201 agcggcaaga cgctgctggc cagggcgatc gccggcgagg cgagggtacc gttcttctcc
     1261 atctccggca gcgagttcgt ggagatgttc gtcggcgtgg gggccgcccg ggtgcgggat
     1321 ctgttcgacc aggcgaagaa gtcgaccccg tgcctcgtgt tcatcgacga gatcgacgcg
     1381 gtgggccggc agcggggcgc ggggttgggc ggcgggcacg acgagcgcga gcagacgctc
     1441 aaccagttgc tggtggagat ggacgggttc gatcccaaca gcgggatcat cgtcatcgcc
     1501 gcgaccaacc ggccggacat cctcgacccg gcgctgttgc ggcccgggcg gttcgaccgc
     1561 cggatcgtcg tggacaaccc ggacacgaag gggcggcggg cgatcctgga cgtgcacgtc
     1621 cggggcaagc cgctc
//
LOCUS       DASQGH010000407         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24544, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000407 DASQGH010000000
VERSION     DASQGH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24544"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VEW91_07975"
     CDS             <1..283
                     /locus_tag="VEW91_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HYM91552.1"
                     /translation="SYTAEVARTRRAAAGGGPHQGGRSEEVRSPMPGLLIAVQAGEGA
                     QVAMGQALVIMEAMKMQMEIRAPHAGTVRRIHVGPGQEIAAGQLLVTME"
     gene            287..1996
                     /locus_tag="VEW91_07980"
     CDS             287..1996
                     /locus_tag="VEW91_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYM91553.1"
                     /translation="MPRRGRRKARRAAAGGKRPLRNARARWEAEVLRPALERTPERRE
                     AFHTASGIPVARVYTAEDAQGIEAERAIGAPGAYPFTRGIYPTMYRGRLWTMRQYGGY
                     AGAEESNRRYRYLLAQGQTGLSVAFDLPTQMGYDSDHPIAQAEVGKAGVAIDSLADME
                     VLLDGIPLDRVSTSMTINATAAILLCMYIAVGERQGVRPDRLDGTTQNDILKEYIARG
                     TYIYPPGPSLRLVTDICAFCAEHLPRWNPISISGYHIREAGATAVQEVAFTLGNAVAY
                     IEGLRRRGLGVDAFAPQLAFFFDAQMDLFEEVAKYRAARRLYARIMRERFGARDPRAQ
                     MLRFHTQTAGVALTAQQPNNNVVRVTLQALAAVLGGTQSLHTNARDEALALPTDEAAL
                     VALRTQQIIAHESGVVATVDPLAGSYYVESLTAEIERRAQAYLDRIDEMGGMLRAIEE
                     GYVQREIGEAAYREQQEVESGRRVIVGQNRFQQPAEHAPAILRVAPEVLARQKARLAR
                     TRAERSGARVRAALAALGEAAKGTENLLPRILECVRAYATIGEICDTLRSRFGTYQPP
                     AVV"
     gene            2002..2430
                     /gene="mce"
                     /locus_tag="VEW91_07985"
     CDS             2002..2430
                     /gene="mce"
                     /locus_tag="VEW91_07985"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HYM91554.1"
                     /translation="MSVRLGHLGIIVHRHDTAAAFYRRLGLVESGRETFSQENVRIAF
                     IRVGDARIELLEPLAPTGPLSRFLDRWGEGIHHLALEVPDLDQALARARAAGVRLIDA
                     SPRQGARGTRVAFLHPDTAHGVLIELIEAPPERSSANPPE"
     gene            2695..3741
                     /locus_tag="VEW91_07990"
     CDS             2695..3741
                     /locus_tag="VEW91_07990"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HYM91555.1"
                     /translation="MAPVFRSVASDIQVLRDPRRPHSIHLVFAMLGFLASGLLAPFVS
                     IPRLSQPVYLSADGRTVSYSSHKRTVEEVLKEVQVAVGTGDRVTPALGTAIWPGIQIT
                     VTRALPLSFTIGGVERQVRLPATTVGEALSLLAVETRPEDRILPDPSTVLTPGMRITV
                     ERREMRRWIERAPIPFATQAVFDRDLFKGQQIVRSPGQPGVRDRTVQAEYADGRVVAL
                     HTIAEAVVQPPAPQIVAVGTRPLIAVLGPFAGKEIMTLEATAYYPGPNNYGGPVGTRT
                     AIGMVARRGVVAVDPSVIKLGTRLHVDGYGDAVAGDTGGAIRGYRIDLCFDTYEEAIR
                     FGRHPVKVYILGTP"
     gene            3814..4665
                     /gene="rsmA"
                     /locus_tag="VEW91_07995"
     CDS             3814..4665
                     /gene="rsmA"
                     /locus_tag="VEW91_07995"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYM91556.1"
                     /translation="MRPSRRWGQHFLVSPRTLDRTLDAASLSRDDSVLEVGAGIGTLT
                     LGLADRAGWVTAVEVDRRLVSALEAIVGPRPNVRIVQGDILALPAEALFDGPAGAPRK
                     VVANLPYNIASAVLTRLLEEPLGLAVLVVTVQREVAERIAARPGGRAYGRLSIAVQYR
                     AVPRIIARISPGAFLPQPAVESALVELRPLPRPAVEVPDEGTFFRVVAAGFAQRRKTL
                     HNSLASVLDQPREVVARALGEAGIDPRARAETLDLAAFGRLAWALAVPLRTAPPPLRP
                     RGRGRRS"
     gene            complement(4680..>5262)
                     /locus_tag="VEW91_08000"
     CDS             complement(4680..>5262)
                     /locus_tag="VEW91_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91557.1"
                     /translation="RRIQRQQDVMQALLDELRRPRIVPRIPRVLRVFREDIQTNLTQD
                     QLITLALFGARLHSGGLIRETLPGRLGEDWVPDIPRDREMVARIFYDVGADTLNRTTV
                     EVVADTARRTAAAEVQARLQALGIKIVRMRTAATLGPTRVILHRGNARVAGLVAGLVG
                     GIAVTGGPRVGGPDLTIFLASAASTAPQLGLPR"
BASE COUNT          763 a         1825 c         1918 g          756 t
ORIGIN      
        1 cagttatacc gccgaggtcg cccggacgcg ccgggccgcg gcgggcgggg ggccgcatca
       61 gggcggacgg agcgaggaag tgcggtcccc gatgcccggg ctgctgatcg ccgtgcaggc
      121 gggtgaaggg gcccaagtcg cgatggggca agccctcgtt ataatggagg cgatgaagat
      181 gcagatggag atccgggcgc cccacgccgg gaccgtcagg cggatccacg tcggtccggg
      241 gcaggagatc gccgcggggc aactgctggt cacgatggag tgacgggtgc cgcggagggg
      301 taggcggaaa gcgcgaaggg cggccgccgg cgggaagcgg ccgctccgca atgcccgggc
      361 ccggtgggaa gccgaggtcc tccggccggc gctcgagcgc accccggagc gccgcgaggc
      421 gtttcacacg gcctccggca tcccggtcgc gcgggtgtac acggccgagg atgcccaggg
      481 gatcgaggcg gagcgggcga tcggcgcccc gggcgcgtac cccttcacgc gcgggatcta
      541 ccccacgatg taccgcggcc ggctgtggac gatgcggcag tacggtgggt atgccggcgc
      601 cgaggagtcc aaccgccggt accggtatct gctcgcgcag ggccagaccg gcctctcggt
      661 ggccttcgac ctccccaccc agatgggcta cgacagcgac cacccgatcg cccaggccga
      721 agtcgggaag gccggggtgg cgatcgactc gctggccgac atggaggtgc tgctggacgg
      781 gatccccctc gaccgcgtca gcacgtccat gacgatcaac gcgacggcgg cgatcctcct
      841 gtgcatgtac attgcggtgg gggagcggca gggcgtgcgg cccgaccggc tggacgggac
      901 cacccagaac gacatcctca aagagtacat tgcccggggg acgtacatct atccgcccgg
      961 cccctcgctg cggctcgtca ccgacatctg cgccttctgc gccgagcacc ttccccggtg
     1021 gaacccaatc agcatcagcg ggtaccacat ccgggaggcc ggcgcgaccg cggtgcagga
     1081 ggtcgcgttc acgctcggca acgcggtggc gtacatcgag gggctcaggc ggcgggggct
     1141 cggggtggat gcgttcgccc cgcaactcgc gttctttttt gacgcgcaga tggacctctt
     1201 tgaggaggtg gccaagtacc gggccgcgcg ccggctctac gcccggatca tgcgggagcg
     1261 gttcggcgcc cgggatccgc gtgcgcagat gctgcggttc cacacccaaa ccgcgggcgt
     1321 ggcgttgacc gcgcagcagc ccaacaacaa cgtggtgcgc gtcacgctgc aggccctggc
     1381 cgccgtgctg ggcgggaccc agtcgctcca cacgaacgcg cgggacgagg cgctcgcgct
     1441 gccgaccgac gaggcggccc tggtggcgct gcgcacccag cagatcatcg cgcacgagag
     1501 cggcgtggtc gccacggtgg atccgcttgc cggatcctac tacgtggagt cgctcaccgc
     1561 cgagatcgag cggcgggcgc aggcctacct ggatcggatc gacgagatgg gcggcatgct
     1621 ccgcgcgatc gaggaggggt acgtgcagcg cgagatcggg gaggccgcct accgcgagca
     1681 acaggaggtc gaatccggcc ggcgggtcat cgtcggccag aaccgcttcc agcaacccgc
     1741 ggagcacgcc cccgcgattc tgcgggtggc ccccgaggtc ctggcccggc agaaggcccg
     1801 gctcgcgcgc acccgcgccg agcgatccgg cgcccgggtg cgggcggcgc tcgccgcgct
     1861 gggcgaggcg gcgaagggga ccgagaacct cctgccccgg atcctcgagt gcgtccgggc
     1921 gtacgcgacg atcggggaga tctgcgatac gctgcggagc cggttcggga cgtatcagcc
     1981 gccggcggtg gtgtaggccg ggtgagcgtg cggctggggc atctcgggat catcgtccac
     2041 cggcatgaca ccgccgcggc attctaccgg cggctcgggc tggtcgagag cgggcgggag
     2101 acgttttcgc aggagaacgt ccggatcgcg ttcattcggg tgggggacgc gcggatcgag
     2161 ttgcttgagc cgctggctcc caccggcccg ctctcccggt tcctggaccg gtggggggaa
     2221 gggattcacc acctggcgct cgaggtgccg gatctcgacc aggcgctggc ccgggcgcgg
     2281 gccgccgggg tccgcctgat cgacgcgtcg ccgaggcagg gcgcccgcgg cacccgggtc
     2341 gcgtttctcc accccgacac cgcccacggc gtgctcatcg agttgatcga agccccgccg
     2401 gagcgatctt ccgcgaatcc tccagaataa ttccccctcc ggacgaagga tagaaaacat
     2461 ccgttcgtgt cgagggaggg gtcccattcc gccgaatcct ttgcattgaa tgcagtctat
     2521 ctgatatagt ggcgggtgta gaatacagtc tatatcacat aatccaggtc tggccgctgc
     2581 cgccgtcatc ggccgagagg atttgcccgg cctcatcttt ctctactgcc agccgggggg
     2641 cgggtcccgc cggggcgaga tcacgtgatg gcgtgcagcc aaaggaggag cggcatggca
     2701 cctgtcttcc gctctgtggc cagtgacatc caggtcctca gagaccctcg ccggccccac
     2761 tcgatccatc tcgtcttcgc gatgctcggg ttcctggcct cgggcctcct tgcccccttc
     2821 gtgtcgatcc cccggctgag ccagccggtc tacctgtcgg ccgatggccg caccgtgtcg
     2881 tacagctcgc acaagcggac cgtcgaggaa gtgctgaagg aggtccaggt cgcggtcgga
     2941 acgggcgacc gggtgacccc cgccctgggg accgcgatct ggcccgggat ccagatcacc
     3001 gtcacgaggg cgcttccgct gagcttcacc atcggcgggg tcgagcgcca agtccggctg
     3061 cccgcgacca cggtcgggga agccctcagc ctgttggccg tcgagacgcg ccccgaggac
     3121 cggatccttc cggacccctc cacggtgctc acgcccggga tgcggatcac ggtcgagcgg
     3181 cgtgagatgc ggcggtggat cgagcgggcc ccgattccgt tcgccaccca ggccgtgttc
     3241 gaccgcgacc tgttcaaggg tcagcagatc gtccggtcgc cgggccagcc cggcgtccgg
     3301 gaccgcaccg tccaggccga gtatgcggac ggccgcgtcg tggcgttgca cacgatcgcg
     3361 gaggccgtcg tccagccgcc ggctccgcag atcgttgccg tcggcacgag accgttgatc
     3421 gccgtcctgg gtcccttcgc cggaaaggag atcatgaccc tcgaggcgac cgcctactat
     3481 ccgggcccca acaactacgg ggggccggtc ggcaccagaa ccgcgatcgg catggtcgcc
     3541 cggcgcgggg tggtggccgt ggacccgtcc gtcatcaagc tggggacgcg cctgcacgtt
     3601 gacgggtacg gggacgcggt ggccggggat accggcgggg cgatccgcgg ctaccggatc
     3661 gacctctgct tcgacaccta cgaagaggcg atccggttcg gccggcatcc cgtgaaggtg
     3721 tacatcctcg gcacaccctg agccacgacg accgagcgat tcgccgatct cgccaccccc
     3781 gccgggaccc gggcggtcct ccggcggttc ggcattcgcc cgtcacggcg gtggggtcag
     3841 cacttcctcg tcagcccccg gacgttggac cggacgctcg atgcggcctc cctgtcccgt
     3901 gacgacagcg tgcttgaggt gggggccggc atcggcacgc tcaccctcgg gctggcagac
     3961 cgggcagggt gggtcaccgc cgtggaagtc gatcggaggc tcgtgtcggc cctcgaggcc
     4021 atcgtcggcc cccggccaaa cgtgaggatc gttcaagggg acatcctcgc gctacccgcg
     4081 gaggcgctct tcgacggccc ggccggcgcg ccgcgcaaag tggtggccaa cctgccgtac
     4141 aacatcgcct cggccgtcct gacacggctc ctggaggagc cgctcgggct cgcggtgctc
     4201 gtcgtcacgg ttcagcggga agtggcggag cgcatcgccg cccgcccggg aggacgcgcc
     4261 tacgggaggc tctccatcgc ggtccagtac cgggccgtgc cccgaatcat cgcgcgcatc
     4321 tccccggggg cctttctccc ccagccggcc gtcgagtccg cgctcgtgga actccggccg
     4381 ctcccccggc ccgcggtcga ggttcccgat gaggggacat tcttcagggt cgtcgcggcc
     4441 ggcttcgccc agcgccgaaa gacgctccac aactccctcg ccagcgtgct cgaccaaccc
     4501 cgggaagtcg tggcgcgcgc cttgggggag gcgggcatcg acccgcgcgc ccgggctgag
     4561 acgctggatc tcgcggcatt cggaaggctg gcgtgggcgc tggcggtgcc gctgcggacc
     4621 gctccgccgc cgctccgccc gaggggaagg gggcggcggt cgtaggatca gcggcccggt
     4681 taccgaggga gcccgagctg aggggcggtc gatgcggcgc tggcgaggaa aatggtcagg
     4741 tcggggccgc ccacgcgcgg gccgccggtc accgcgatcc cgccgacgag cccggccacg
     4801 agcccggcca cgcgcgcgtt cccccggtgc aggatcaccc gcgtgggccc cagggtggcc
     4861 gcggtccgca tccggacgat cttgatcccg agtgcctgga gccgggcctg gacctcggcc
     4921 gcggcggtgc ggcgggccgt atccgcgacc acctcgaccg tggtgcgatt cagcgtgtcc
     4981 gccccgacgt cgtagaagat gcgcgccacc atctcgcggt cgcgggggat gtccggcacc
     5041 caatcctccc ccagtcggcc gggcagggtc tcccggatca gcccgccgga atgcagccgg
     5101 gccccgaaga gggcgagggt gatcagctgg tcctgcgtca gattcgtctg gatgtcctcg
     5161 cggaagaccc ggagcacgcg ggggatccgg ggaacgatcc ggggccggcg tagctcgtcg
     5221 aggagcgcct gcatgacgtc ctgctggcgc tggatccgtc gg
//
LOCUS       DASQGH010000408         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1725, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000408 DASQGH010000000
VERSION     DASQGH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1140)
                     /locus_tag="VEW91_08005"
     CDS             complement(133..1140)
                     /locus_tag="VEW91_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91558.1"
                     /translation="MGQYLIRRLALIVVTLWGLSVLTFAISRIAPGDPARLAAGPQAT
                     EAMVAHLRHEYGLDRPLWVQYLRYLDQVGRGNLGVSLMTARPVRADLGLYFGATLELV
                     LFSLLFATVTGVILGVLSAVYHNRLVDHASRLVAITGLGMPEFWLALMLQLALASHYR
                     LLPLGGQLSLGIVPPPPVTRLYLADSLLAGQGATFLSALQHLILPAVALSFPALASII
                     RISRADMLDTLPREFVRTARAKGLPPQTVILRHALRNALLSSVTMIGLRFGYMLGGTV
                     LVEKVFDWPGIGLYMVNAAAASDFEPVMGVTLVVGLSFALANLLVDLGYGVLDPTIRY
                     A"
     gene            complement(1172..>2759)
                     /locus_tag="VEW91_08010"
     CDS             complement(1172..>2759)
                     /locus_tag="VEW91_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91559.1"
                     /translation="RQLLVRAGVGASAGLALGGLGGEMRPADAYLTRAGKKILVVGAA
                     QEVPNLDPSLHYDWSTRMLQQSTYDALLKYVGDPAQITPWLATGYEASPDAKTWTFHL
                     DRRARFHNGDPVDSAAVKFSYTRTIELKQGPAWTLQGVLEPADIEMPNPSTLVFHLKK
                     PFAPFAAIVPWWYIMNPREVRAHEQGNDYGQAWLREHDVGSGPFRIASWQPNTAYELE
                     AVPDYWKGWPSQGHLDGFIHQVMREPSTQKLAIQRGDIDVTTNIVPEDLDLLAHAPGT
                     YVTENRGITTFGVKMNNQRGPTANKALRKAIAYAFNYKDLINIYKGHAILENSPVPIG
                     MRGHVDLPPYHQDLERARGFLREAGVSVPVRLTYVYVDGFTEEKEIGLALKASLDPVG
                     INVELIGRAWPTMVGMGTKPETMPDMMAVFVTPLYNDPDAVVSQYTPTAWGQYYGVNF
                     YKHEEVTSLVERARSIADWSTRVTLYDRIQRVLWEDSPEVFGMQYMRRWAFRSVVKGF
                     QFCPLRQTNEIDMYSVYIQG"
BASE COUNT          458 a          917 c          954 g          430 t
ORIGIN      
        1 acgattccca gcccgagcgt ggtgagccgg tttcggaaga accggcgccc cgcccgctgc
       61 caggcgacat cgcgcgcacg cgggtgggtg agggttccgg tccccggccg cagatccgcc
      121 gcggtggcgc cctcacgcat accggatcgt gggatccagc accccgtacc cgaggtccac
      181 gagcaggttg gcgagggcga acgacaggcc aacgacgagg gtgaccccca tcaccggctc
      241 gaagtcgctc gccgcggccg cgttcaccat gtacaggccg atcccgggcc aatcgaacac
      301 cttctcaacg agcacggtgc cccccagcat gtacccgaac cggagcccga tcatcgtgac
      361 agaggacaat agcgcgtttc gcaacgcgtg gcgaaggatg accgtctgcg gggggagtcc
      421 cttggcccgc gccgtgcgga cgaactcccg cggcagcgta tccagcatgt ccgcccggct
      481 gatccggatg atcgaggcga gggccggaaa cgagagcgcg accgccggaa ggatgaggtg
      541 ctggagggcg ctgagaaacg tcgccccctg ccccgccagc aggctgtcgg ccaggtacag
      601 gcgggtcaca ggcggcgggg ggacgatccc gaggctgagc tgtcccccga gcgggaggag
      661 gcggtagtgg ctggcgagcg cgagctgaag catgagggcc agccagaact cgggcatgcc
      721 gagtccggtg atcgcgacga ggcgcgatgc gtggtccacc agccgattgt gatagacggc
      781 cgacaggacc cccaggatga caccggtcac cgtcgcaaag aggagcgaga acagcaccag
      841 ctccagggtg gcgccgaagt agagcccgag gtcggcgcgg accgggcgcg cggtcattag
      901 ggaaacgccc aggttgcccc ggccgacctg gtccaggtag cggaggtact ggacccatag
      961 ggggcggtcg agcccgtact catgccggag gtgggcgacc atcgcttcgg tcgcctgcgg
     1021 cccggcggcc agccgcgctg gatccccggg ggcgatccgg gagatggcga aggtgagcac
     1081 ggaaagcccc cagagggtca ccacgatcag cgcaaggcgc ctgatcaggt actgccccat
     1141 gcccggacgc cggatgcgag ctccggcgcg atcagccctg gatgtagacc gagtacatat
     1201 cgatctcgtt cgtctgccgc agcgggcaga actggaagcc tttcaccacc gagcgaaacg
     1261 cccaccggcg catgtactgc atcccgaaga cctccgggct gtcctcccag aggacccgct
     1321 ggatcctgtc gtagagggtc acccgggtgc tccagtcagc gatcgaccgc gcccgctcga
     1381 caagcgacgt cacctcttcg tgcttgtaga agttgacgcc gtagtactgc ccccacgcgg
     1441 tgggcgtata ctggctgacc acggcgtcgg ggtcgttgta cagcggggtg acgaacacgg
     1501 ccatcatgtc cggcatcgtc tcgggcttgg tgcccatccc caccatcgtc ggccaggccc
     1561 ggccgatgag ctcgacgttg atgccgaccg gatcgaggct ggccttgagg gccagcccga
     1621 tctccttctc ctccgtaaag ccgtcgacgt agacgtaggt cagacggacg gggaccgaca
     1681 ccccggcctc ccggaggaac ccccgggcgc gctcgaggtc ctggtggtat gggggaagat
     1741 cgacgtgccc ccgcatcccg atcggcaccg ggctgttctc gaggatcgcg tggcccttgt
     1801 agatgttgat caggtccttg tagttgaacg cgtacgcgat cgccttccgg agcgccttgt
     1861 tggccgtcgg cccgcgctgg ttgttcatct tgacgccaaa ggtggtgatc ccccggttct
     1921 ccgtcacgta cgttccgggg gcgtgcgcca acaggtccag atcctcgggg acgatgttgg
     1981 tcgtcacgtc gatgtccccc cgctggatcg ccagcttctg cgtgctgggc tcccgcatga
     2041 cctggtggat gaatccgtcc aggtggccct gggacggcca ccccttccag tagtcaggga
     2101 cggcctcgag ttcgtacgcg gtgtttggct gccaggaagc gatgcggaac ggcccgctgc
     2161 cgacatcgtg ctcccgcagc caggcctggc cgtaatcgtt gccctgctcg tgggcccgaa
     2221 cctccctggg gttcatgatg taccaccagg ggacgatcgc cgcaaacggc gcgaagggct
     2281 tcttcagatg gaagacgagc gtcgatgggt tgggcatctc gatgtcggcg ggctccagga
     2341 cgccctggag cgtccacgcc ggcccctgct tcagctcgat tgtccgggtg tacgagaact
     2401 tgaccgcggc actgtccacg gggtcgccgt tgtggaaccg ggcgcggcgg tccaggtgaa
     2461 aggtccacgt cttcgcatcc gggctggcct cgtaccccgt ggccagccac ggggtgatct
     2521 gggcgggatc gcccacgtac ttgaggagcg catcgtacgt ggattgctgg agcatgcggg
     2581 tcgaccagtc gtaatggaga ctcgggtcca ggttggggac ctcctgcgcc gcccccacca
     2641 cgaggatctt cttgccggcc cgggtgaggt aggcgtcggc cgggcgcatc tcgcccccaa
     2701 gcccgccgag cgccagcccc gcggaggcgc ccacgcccgc ccgtaccagg agctgccgc
//
LOCUS       DASQGH010000409         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49877, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000409 DASQGH010000000
VERSION     DASQGH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49877"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(95..181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            375..2630
                     /locus_tag="VEW91_08015"
     CDS             375..2630
                     /locus_tag="VEW91_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYM91560.1"
                     /translation="MTLEQILAALRTRPPFASQLTDWRTFPEVPARYEGFPPDLDPRL
                     AASLERRGIRHLYTHQAEAYRAVARGADIVVVTPTASGKTLCYNLPVVDAILKRGDAR
                     ALYLFPTKALSGDQVDELHGLVEGLGGDLRTSTYDGDTPHEARRTIRAAGHIVVSNPD
                     MLHAGILPHHTKWLRLFENLRYVVLDELHTYRGVFGSHVANVLRRLLRVCRFYGSAPQ
                     FICCSATIANPREHAERLIGRPVTLIDESGAPRGPRTIGLYNPPVVNHSLGIRRDAVR
                     EAVALAGELLAGRVQTIFFARTRLGAELLTTYLQGDARRRGQDPGAICGYRAGYLPSE
                     RRRIEAGLRSGVIRAVAATNALELGIDIGQLAASVLVGYPGTIASTWQQMGRAGRRGE
                     PSLAILVGTSAPLDQFIVTHPEYLFSHPVEEARTNPDNLLILTSHLKCAAFELPLRDE
                     EAFGPSTLPEILAYLEEERVLHHDGDAWYYVAEAYPAEEVSLRSASTENVVIIDTSDP
                     RPRVIGEVDLASAPAFVHEDAIYLHLGQQHHVERLDWEERKAYVRRVNVDYYTDAQIA
                     LGVRVLEEFARTAEAAHGEVVVTFRPTIFKKLKLSTHENVGFGPIRLPETTLHTTAAW
                     WVFPRSRASVLSPDELQGALLGVAHALGNVSPLYLMSDPRDLGSVAEIKSAHTAEPTV
                     TLFERVPGGVGQAERLYTLGSELLAAARALVEACGCAEGCPSCVGPALEVGERGKQHT
                     LELLRGACVPA"
     gene            2617..>3516
                     /locus_tag="VEW91_08020"
     CDS             2617..>3516
                     /locus_tag="VEW91_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HYM91561.1"
                     /translation="MSPPDPALRDRLRRLGAAGMGGTRLRPPSPAPSPGTTRGVGEGI
                     EVIRARFGLGHRHGHRALGECVDALTDHEAGADPSHTLWLDTETTGLAGGTGTHVFLV
                     GLGSVQGAAFVIEQFLLRRLSAEGQFLSLLQARLAGAHVVTFNGQRFDWPLLEARWIL
                     TRLRPAAIELHTDLIHPARRLWHRVLGTHRLSAIEAAVLGAPRPDDIPGWMIPQVYVH
                     YLRTADRTALESILAHNRADLLALLGLHAQVARVLRDPRIAGPIDWEGAGVLLARRGD
                     HGQAALCFERALQEPLEAGDQWRV"
BASE COUNT          516 a         1269 c         1202 g          529 t
ORIGIN      
        1 taggagaagc cgaaggcggg taacgccagc gccaccacca ctcccacgag ccgtgccatc
       61 cagatccgat ccatgcacag cctcccgctc ggcgcccggc agcccggctg acttgccaga
      121 tccgctggcc tgtcccgtgg ctttgcgtcc ccaggtctcc ctgggtttgc cacctacgag
      181 cgcgcgattg tttgtggcac ccctataggt gccccgaaaa ctgctaggga agaccccacg
      241 acgctctcag gagtctcccg gaaggcccgg aggagttcgc cgagccgatg gcgaacacgt
      301 gttcgcggcg tattttttga tggatttccg ccccgctgcg agggtcatca cgccgcttca
      361 ggagtggtga gacaatgacg ctcgaacaga tcctcgccgc gttgcgcacc cggccgccat
      421 ttgcctcaca gttaaccgac tggcgcacat tccccgaagt cccggccagg tacgaggggt
      481 tcccccctga tctggatccc cggctggccg cttccctcga gcggcggggg atccgacacc
      541 tgtacacgca ccaggcggag gcgtaccgcg cagtcgcgcg cggggccgac atcgttgtcg
      601 tgaccccgac cgcctcgggc aagacactct gctacaacct cccggtggtg gatgccatcc
      661 tcaaacgcgg cgatgcccgc gcgctgtacc tgttcccgac caaggcgctc tcgggcgacc
      721 aggtcgacga gctccacggc ctcgtcgagg ggctcggagg ggacctccgg acgtcgacct
      781 acgatggcga cacgccccac gaggcgcggc gcacgatccg ggcggccggg cacatcgtgg
      841 tctcgaaccc ggacatgctg cacgcgggga tcctgccgca ccacacgaaa tggctccgcc
      901 tgttcgaaaa cctgcggtac gtggtcctcg acgagctcca cacctaccgg ggggtgttcg
      961 ggtcccacgt cgccaacgtg ctccgccggc tcctgcgggt ctgccgtttc tatgggagcg
     1021 cgccgcagtt catctgctgc tccgccacga tcgccaaccc gcgcgagcat gcggagcggc
     1081 tgatcgggcg cccggtcacc ctgatcgacg agagcggggc cccccgcggc cccaggacga
     1141 tcgggctgta caaccctccg gtcgtcaacc actctctcgg catccgccgc gacgccgtgc
     1201 gggaggcggt ggcgctcgcc ggagaattgc tggcggggcg ggtgcagacg atcttctttg
     1261 cgcgcacccg cctcggcgcc gagttgttga cgacctacct ccagggcgac gcccgccggc
     1321 gggggcagga tcccggggcg atctgcggct accgggccgg gtaccttccc agcgagcgcc
     1381 ggcggatcga ggccgggctc cgcagcggcg tgatccgggc cgtggcggcg acgaacgcgc
     1441 tggaactcgg catcgacatc gggcagctgg ccgcctcggt gctcgtgggg tatcccggga
     1501 cgatcgccag cacgtggcag cagatgggac gggcgggccg gcgcggcgag ccctcgctgg
     1561 cgatcctggt cggcacgagc gccccgctcg accagttcat cgtcacccac cccgagtatc
     1621 tgttttccca tccggtggag gaagcccgga ccaaccccga caacctgctg atccttacca
     1681 gccacctgaa gtgcgccgcg ttcgagctgc cgcttcgcga cgaggaagcg ttcggcccga
     1741 gcacgctccc ggagatcctc gcctatctcg aggaggagcg cgtgctgcat cacgacgggg
     1801 acgcctggta ctacgtcgcc gaggcctacc ccgcggagga ggtcagcctc cggagcgcca
     1861 gcacggagaa cgtcgtcatc atcgacacga gcgatccccg cccccgcgtg atcggcgagg
     1921 tggacctcgc ctccgccccg gcgttcgtcc acgaggacgc gatctacctc cacctgggcc
     1981 agcagcacca cgtcgagcgc ctcgactggg aggaacgcaa agcctacgtc cgccgggtga
     2041 acgtcgacta ctacacagac gcccagatcg cgctgggcgt ccgggtgctg gaggagtttg
     2101 cccggacggc ggaggccgcc cacggcgagg tggtggtcac gttccggccg acgattttca
     2161 agaagctcaa actctccacc cacgagaacg tcggcttcgg cccgatccgc ctcccggaga
     2221 cgaccctgca caccacagcg gcgtggtggg tcttcccccg atcccgggcc tccgtgttga
     2281 gcccggacga actccagggg gcgctcctgg gggtcgccca cgcgctcgga aacgtctcgc
     2341 cgctctacct gatgtccgat ccgcgggatc tgggatcggt ggccgagatc aagtccgccc
     2401 acaccgccga gccgacggtg acgctgttcg agcgggttcc cgggggcgtc ggacaggcgg
     2461 agcggctgta cacgctgggc agcgagttgc ttgccgccgc gcgcgccctg gtggaagcgt
     2521 gcgggtgcgc cgagggatgc ccctcctgcg tggggccggc cctggaggtc ggggaacggg
     2581 gcaagcagca tactctcgaa ctcctgagag gcgcctgtgt ccccgcctga ccccgccctc
     2641 cgtgacaggc tccggcgcct cggcgcggcc gggatgggcg gcacccggct gcgcccgccg
     2701 tccccggcgc cgagccccgg gacgacccgg ggggtggggg agggtatcga ggtcatccgc
     2761 gcgcggttcg ggcttgggca ccggcacggg caccgggcgc tgggggagtg cgtcgacgcg
     2821 ctgaccgacc acgaagcggg cgccgacccg tcgcacacgc tgtggctgga caccgaaacc
     2881 acgggcctgg ccggcggcac gggcacgcac gtttttctcg tcgggctggg ctccgtccag
     2941 ggcgccgctt ttgtgatcga gcagttcctc ctcaggcggc taagtgcgga gggccagttc
     3001 ctctccctcc tacaagctcg attggccggg gcgcatgtcg tgaccttcaa cggccagcgg
     3061 ttcgactggc cgctgctcga ggcgcggtgg atcctcaccc ggctccggcc ggcggcgatc
     3121 gagctccaca ccgacctcat ccatcccgcc cgacggttgt ggcaccgggt gttgggcacc
     3181 caccgcctga gcgcgatcga ggcggcggtg ctcggcgccc cccgcccgga tgacatcccg
     3241 gggtggatga tcccgcaggt gtacgtccac tacctgcgca ccgccgaccg caccgcgctc
     3301 gagtcgattc tggcgcacaa ccgggcggat ctcttggcgc tcctcggtct ccacgcccag
     3361 gtggcgcgcg tcctgcgcga tccccgcatc gccgggccga tcgactggga gggagctggg
     3421 gtgctcctcg cccgacgggg ggaccacggg caggccgcgc tgtgcttcga gcgggcgctc
     3481 caagaaccgc tcgaggccgg ggatcagtgg agggtg
//
LOCUS       DASQGH010000410         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7561, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000410 DASQGH010000000
VERSION     DASQGH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7561"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VEW91_08025"
     CDS             <1..552
                     /locus_tag="VEW91_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HYM91562.1"
                     /translation="IYLGDRASSVRAQLIGVQKKLDALGPIDAQVKQLEQTVAVLQAR
                     QAALKQLLTNQLPASESLEAIKTVIPRDVWLVNVSTQGGHNVLFDGSTFSYKSVARFM
                     VALRDSERFRNIDLTSTQKDRVGQREVVKFSVTGELVTGPTKGRELVTPTAGAWDTPT
                     QPVASHQGTPSPGRISSTTGDPQ"
     gene            549..1196
                     /gene="pilO"
                     /locus_tag="VEW91_08030"
     CDS             549..1196
                     /gene="pilO"
                     /locus_tag="VEW91_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HYM91563.1"
                     /translation="MKPLQAREKTILWVLAMLVVVVGFYNFVYSPKTKEYATLAVQLK
                     AKQEELTRVQAQAARKDELERRMQELQRDLQETEARLPSSKEIPVLLVQLERLAGQVG
                     ANLTLIRPGPPQQAPQPPAGQPALRPGGAAPSGPSGPPSVGLVPFGLELNAEGTYDTV
                     QSFLRGIETFPRFIAMSDLRISPLPPKPGESALQPRLSVGVSATTYFVAESGAGR"
     gene            1193..1975
                     /locus_tag="VEW91_08035"
     CDS             1193..1975
                     /locus_tag="VEW91_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91564.1"
                     /translation="MKPRGSNKTLVRLASVGFLLVLAGVLVYLSMAARAPRALTRPGA
                     PRVATPVRPAPVVPPTMSPATPPAGPSSAGGPAPAGIAPRAAGTPPVAGTAAPSGAPA
                     MGGPPKATPPAQAPPGKPPSGTAGAPINVASTEVNPNSEVALVGKGPEGRSDPFSPLA
                     VQESGGPSPLLPPPPGVGLPMPPGFVAPGGGPGAGALGMRVAGIMGGRSRVAIIEASD
                     GKSYIVRAGERVGDAVVISVLPDKVVIKQNNQTFELSFGGAS"
     gene            1972..>3694
                     /locus_tag="VEW91_08040"
     CDS             1972..>3694
                     /locus_tag="VEW91_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HYM91565.1"
                     /translation="MIVPLRDSLRSVAFGVALILAFAAWPVSQAEAAPVKVTGVALKE
                     AANELQVSIVATGPVRYQTRDVQPGWIVVDVQDARLGMPSGELPLARGLVRKVRVGQF
                     TPDVVRVVVELRQPARFRLTASPDNAAILLGIPKPVGEQPVPPAAEMAQLPLPPAAPP
                     AAGSISDQNLINLDLRNADIADVISALAKLANVNVVTDSDVKGKITVRLNSVTFEDAL
                     RLVLEPNGLGYATVGPNLVVMKKEKLARPVLRQYQIANMSAGQFVGGYLPVIGIKKDQ
                     AVVDDATNSILVVGTVEEQARISDLISRVDLPVEKVVTRVIKLNYIDAAGFVDLMGAR
                     LPDAVAKSAKVDKASNSIVLTASQAQMDLVDALLQQVDTPLPQVLVEAMVLEVPTEVT
                     QNLGVAWQSSTPFTITFTGGQYPCCAQVSITSPALLTTLNTLIQENKSRLLANPRLAV
                     RDGETARMNIGDKIPFQVVNAQGVPSVVIIEAGVKLEVTPRINGDGFITVRMHPEVSS
                     IKTAPAAGGVPPTIATREADSSLTVKDGTPIVLAGLIQKNEISTTVKVPLLGDIPILG
                     WLFKSVST"
BASE COUNT          597 a         1263 c         1255 g          579 t
ORIGIN      
        1 atctacctgg gcgatcgggc gtcgtccgtc cgcgcgcagt tgatcggcgt acagaagaag
       61 ctcgacgccc tcgggccgat cgacgcgcag gtcaaacagc tcgagcagac cgtcgccgtc
      121 ctccaggccc gtcaggcggc gctcaagcag ttgctgacga accagctgcc ggcctcggag
      181 tcgctcgagg cgatcaagac cgtcatcccg cgtgatgtct ggctggtcaa cgtctccacg
      241 cagggcggac acaacgtgct gtttgatggg tccacgttct cctacaaatc cgtggcgcgc
      301 ttcatggtgg cgttgcggga ctcggagcgg ttccggaaca tcgatctcac gtcgacccag
      361 aaggatcggg tggggcagcg cgaagtggtg aagttttcgg tcaccgggga gctggtgacc
      421 ggtccgacca agggcaggga gctggtgacc cccacggccg gtgcctggga tactcccacg
      481 cagccggtgg cgtcccacca ggggaccccg tcccccggcc ggatctcgtc caccacgggg
      541 gacccgcaat gaagccgctg caggcgcgcg agaaaacgat cctgtgggtg ctcgccatgc
      601 tggtggtggt ggtgggcttc tacaacttcg tctactcgcc gaagacgaaa gagtacgcca
      661 cgctcgcggt gcagttgaag gccaagcagg aggaactgac gcgcgtgcaa gcgcaggcgg
      721 cgcgcaagga cgaacttgag cggcggatgc aggagctgca gcgcgacctt caggagaccg
      781 aggccaggct tccttcgagc aaggagatcc cggtgctcct ggtgcagctc gagcggctcg
      841 ccggacaggt cggggcgaat ttgacgctga tcaggcccgg ccccccgcag caggccccgc
      901 agccgccggc cggccagccg gcgcttcggc ctggcggagc ggcgccctcc ggaccgtctg
      961 ggccaccatc ggtggggctg gtgccgtttg gcctggaact taacgccgag ggcacctacg
     1021 atacggtgca aagcttcctg cgggggatcg agacgttccc gcggttcatt gccatgtccg
     1081 acctgaggat cagtccgctg ccgccgaagc ccggggagag tgccctgcag ccccggctgt
     1141 ccgtgggagt gtccgccacc acctactttg tggctgaaag cggtgcaggc cgatgaagcc
     1201 gcgagggtcg aacaagacgt tggtgcgcct tgcgtcggtg ggcttcctcc tcgtgcttgc
     1261 cggcgtgctg gtgtatctca gcatggccgc acgagcgccg cgcgcgctca cccggcccgg
     1321 cgccccccgc gtggcgacac cggtccggcc ggctccggtc gtgcccccca cgatgtcacc
     1381 cgccacaccc ccggctggtc cgagcagcgc gggcggcccg gctccggccg gaatagcccc
     1441 cagggccgcc gggaccccgc ccgttgcggg cacggcggct ccctccggag ccccggccat
     1501 gggggggccc ccgaaggcca ccccgccggc ccaggcgcct ccgggaaagc caccttccgg
     1561 gacagccggc gcgccgatca acgtggcttc aaccgaggtg aatcccaaca gcgaggtcgc
     1621 cctggttggc aagggccccg aggggcgctc cgatccgttt tcgcccctcg cggtgcagga
     1681 gtcgggcgga ccgtcgccgc tgcttccgcc gccgcccggc gtggggctcc caatgccgcc
     1741 ggggtttgtc gccccggggg gcggcccggg cgcgggtgcg cttgggatgc gggtcgcggg
     1801 gatcatgggg ggccggtccc gggtggccat catcgaagcc tccgatggca agagttacat
     1861 cgtccgcgcc ggggagcgcg tcggtgatgc ggtcgtcatc tcggtcctgc cggacaaggt
     1921 cgtgatcaag caaaacaatc agaccttcga attgagcttt ggaggtgcat catgatcgtc
     1981 ccgttgcggg attccctgcg atccgtcgcg ttcggagttg cgctgatcct cgcgttcgcc
     2041 gcgtggccgg tgagccaggc cgaggctgct cccgtcaagg tgaccggggt ggccctcaag
     2101 gaggcggcga acgagctcca ggtgtccatc gtcgcgacgg gacctgtgcg ctaccagacc
     2161 cgcgatgtcc agcccgggtg gatcgtcgtg gacgtccagg acgcccgact cgggatgccc
     2221 tcgggtgagc tgcccctcgc ccgcggcctg gtcagaaagg tgcgggtggg gcagttcacc
     2281 cccgatgtgg tccgcgtcgt cgtggagctc aggcagccgg ccaggttccg actcacggca
     2341 tcgcctgaca acgcggcgat tctcctcggg attcccaagc cggtcgggga acaacccgtc
     2401 cccccggcgg ccgagatggc ccagctcccg ctcccccctg ccgcgccacc cgctgcggga
     2461 tcgatctctg atcagaacct gatcaacctc gacctccgta acgccgatat cgccgatgtc
     2521 atctccgccc tggcgaaact ggccaacgtc aacgtcgtga ccgactccga cgtgaagggc
     2581 aagatcaccg tccgcctcaa cagcgtgacc ttcgaggacg cgctccgcct ggtgctggag
     2641 ccgaacggcc tggggtatgc gaccgtggga ccaaacctcg tggtcatgaa gaaggagaag
     2701 ctcgcgcgcc cggtcctccg ccagtaccag atcgcgaaca tgtcggcggg gcagtttgtc
     2761 ggcggctacc tccccgtgat cggcatcaag aaggatcagg cggtggtcga cgacgcgacc
     2821 aactcgatcc tggtcgtcgg cacggttgag gagcaggcga ggatctcgga tctcatctcc
     2881 cgggtggacc tgcccgtcga gaaagtggtc acccgggtga tcaagctcaa ctatatcgac
     2941 gccgccgggt tcgtcgacct gatgggtgcc aggctcccgg acgccgttgc gaagtcggca
     3001 aaggtcgaca aagccagcaa ctcgatcgtc ctgaccgcct cgcaggcgca gatggacctg
     3061 gtcgacgcgt tgctccagca ggtcgacacg ccgctgccgc aggtgctggt cgaggccatg
     3121 gtcctcgagg tgcccaccga ggtgacccag aacctcggcg tggcgtggca gtcgtcgacc
     3181 cccttcacga ttacctttac cggcggccag tacccgtgct gcgctcaggt gtcgatcacc
     3241 tcgccggccc tcctcacgac ccttaacacg ctgatccagg agaataagtc gaggctgctc
     3301 gccaaccccc ggctcgccgt ccgcgacggg gagacggcga ggatgaacat cggcgacaag
     3361 atcccgttcc aggtcgtcaa cgcccagggc gtgccctcgg tggtcatcat cgaggccggg
     3421 gtcaagctgg aggtcacccc gcggatcaac ggcgacggct tcatcaccgt gcggatgcac
     3481 ccggaggtca gcagcatcaa gacggccccg gccgccggcg gcgtcccgcc gacgatcgcc
     3541 acccgtgaag ccgactcgtc gctgaccgtc aaggacggca cgccgatcgt gctcgccggg
     3601 ctcatccaga agaacgagat cagcacgacg gtgaaggtac cgctgctggg ggatatcccg
     3661 atcctgggct ggctcttcaa gagcgtgtcc accg
//
LOCUS       DASQGH010000411         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_15901, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000411 DASQGH010000000
VERSION     DASQGH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15901"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VEW91_08045"
     CDS             complement(<1..533)
                     /locus_tag="VEW91_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91566.1"
                     /translation="MNPVITHHPAIADGQSALSSTGVGVRQLVLVANSPGEVSYLAIP
                     MARAARDWAGSRGLPLAVSLVLPPCQWASGQEAVVARRAGVFERIYSPWDCLAFAARL
                     RRFPAPGPGAVLHFGGDLWYSATLARRFGFSVFAYVESTRPILPRARWFARVFLPSTA
                     LADRLVAEGAPRERLSVV"
     gene            complement(530..1456)
                     /locus_tag="VEW91_08050"
     CDS             complement(530..1456)
                     /locus_tag="VEW91_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91567.1"
                     /translation="MMPIRIADPYAVPGRYRKAQLHCHTTNSDGAFAPQGLLERYRAA
                     GYTFVVFTDHERVTVCEDLNDGTFLALPGVETAVGRPFWPLGPHLGRLGAPGPLHVRG
                     AQAGIDATVAAGGVISLHHPGWTGNLGTGKWSVAEMIALRGYHLIEISNHHSPSGADV
                     EGWAMTLARRGPTAPVAGVAVDDLHRGRDFNTGWVMVKTDAVTPDAFLGALRGLALYA
                     STGPHAEFGARDGAIVCATDAKRIRFLDAHSRVRREASGPEAAYVPEGSEGFVRVECL
                     DAAGRAAWSQAFWIAHEASGLRPPSAKSIIGP"
     gene            complement(1485..2345)
                     /locus_tag="VEW91_08055"
     CDS             complement(1485..2345)
                     /locus_tag="VEW91_08055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYM91568.1"
                     /translation="MRTRSRWVALVATAIAVVLIPLAPPAPPAQAQLFGISEQEENRI
                     GRQVEAEVARKPGFVNDPALTQYVAGIGLRLARVSERPNLPWTYHIIRDPSVNAFAAP
                     GGFIFVYQGLLQFRQSEEELAFVLGHETTHVAHRHAVDLAQRDMELQFGAILITQILF
                     GGSLTAYQLSQLARALIDASYSRDKEFEADHYGVIYAQKAGFDPTGSIGFFERLQSQE
                     KAQPGLVRAFEGHPDTPDRIKALRAQLRQMGYQVVGPADAAPPAEEAPPPKPAVRPSP
                     TAPLRPPGDR"
     gene            2625..>2759
                     /locus_tag="VEW91_08060"
     CDS             2625..>2759
                     /locus_tag="VEW91_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYM91569.1"
                     /translation="MKPKRTTVYLEPDLHKALKVKAAETDQSVSDLISGAVRRSLLED
                     A"
BASE COUNT          443 a          970 c          944 g          402 t
ORIGIN      
        1 accaccgaga gccgctcccg cggggccccc tcggcgacca accgatccgc gagggccgtc
       61 gacgggagaa acacgcgcgc aaaccagcgc gcgcggggga ggatcggccg ggtggattcg
      121 acgtaggcga acacggaaaa gccaaaccgc cgggccagcg ttgccgagta ccacaggtcg
      181 cccccgaagt gcagcacggc ccccggcccc ggcgccggga accgccggag gcgggcggca
      241 aacgccaggc agtcccacgg gctatagatg cgctcgaaga ccccggcccg ccgggcaaca
      301 acagcctcct ggccgctggc ccactggcac ggtggcagga cgagcgaaac ggccaggggg
      361 agcccccggg atccggccca gtccctggcg gcccgggcca tcgggatggc caggtaggac
      421 acctcgccgg ggctgttggc gacgagaacg agttgccgga cgcccacccc cgtgctcgac
      481 aacgcgctct gaccgtccgc gatggcgggg tggtgggtaa tgaccggatt cacggcccga
      541 ttatactctt ggccgagggg gggcgtaaac cgctcgcttc gtgcgcgatc cagaacgcct
      601 gcgaccaggc ggcccggccc gcggcgtcca ggcactccac gcgcacgaac ccctcgctcc
      661 cctcgggcac gtacgcggcc tcagggcccg atgcctcgcg ccgcacgcgg ctatgggcgt
      721 cgaggaaccg gatccgcttc gcgtccgtgg cgcagacgat tgccccatcg cgcgcgccga
      781 actccgcgtg cggccccgtc gaggcgtaca gcgcgagccc gcgcagcgcg ccgagaaacg
      841 cgtccggggt gacggcgtcg gtcttgacca tcacccaccc ggtgttgaag tcccgacccc
      901 ggtgcaggtc gtccaccgcg acgccggcaa ccggggcggt ggggccgcgg cgggcgagcg
      961 tcatcgccca cccctccacg tcggccccgc ttgggctgtg gtggttgctg atctcgatca
     1021 gatgataccc gcgcaatgcg atcatctcag ccacggacca cttccccgtc cccaggttgc
     1081 ccgtccaccc tgggtggtgc agggagatga cgccgccggc cgcaacggtc gcgtcgatgc
     1141 cggcctgcgc gccgcggaca tgaagcggcc ccggggcccc gagccgtccc aggtgcgggc
     1201 ccagcggcca aaacggtcgt ccgaccgcag tctccacccc cgggagggcc agaaacgtcc
     1261 cgtcgttgag gtcctcgcac acggtcaccc gctcgtggtc ggtgaacacc acgaaggtgt
     1321 acccggcggc ccggtaccgc tcgaggagtc cctggggggc gaaggcgccg tcggagttgg
     1381 tcgtgtggca gtgcagttgc gccttgcggt accggccggg gaccgcgtac ggatcggcga
     1441 tcctgatcgg catcacccgc gagagattcg cgggccggcc cgggttacct gtcgccggga
     1501 ggacgcaggg gggcggtggg cgaggggcgc acggccggct tcgggggtgg cgcctcctct
     1561 gccgggggcg cggcgtccgc ggggccgacg acctggtagc ccatctgccg gagttgcgcg
     1621 cggagggctt tgatccggtc gggtgtgtct ggatgaccct caaacgcgcg caccaggccg
     1681 ggctgcgcct tctcctggct ctgcaggcgc tcgaagaagc ctatcgagcc ggtgggatcg
     1741 aacccggcct tctgcgcata gatcaccccg tagtggtcgg cctcgaattc cttgtctcgc
     1801 gagtaactcg cgtcgatcag cgcgcgcgcg agctgggaca gctggtaggc ggtcaggctt
     1861 ccgccaaaga gtatctgcgt gatcaggatc gcgccaaact ggagctccat gtcgcgctgg
     1921 gccagatcga ccgcgtggcg gtgggccacg tgggtcgtct catgtccgag cacaaacgcc
     1981 agctcttcct cggactggcg aaactgcagc agcccctggt agacgaaaat gaaccccccg
     2041 ggggcggcga atgcgttgac gctcggatcg cggatgatgt gataggtcca cgggaggttc
     2101 ggccgctccg aaacacgggc caatcgcagc ccgatccccg ccacgtactg ggtgagggcg
     2161 gggtcgttga cgaaccccgg ctttcgcgcc acctcggcct cgacctggcg cccgatccgg
     2221 ttctcctcct gctcgctgat gccgaagagc tgagcctggg cgggcggcgc aggcggagcc
     2281 agcgggatca acaccaccgc gatcgcggtg gccacgagcg cgacccatcg ggaccgggtt
     2341 cgcatcctcc ccatcctacc ctgctaacgc tccgtccccg cgagaggttc cccgcgccgc
     2401 gctcaacccg aatgcatcac gaagctctac gttcactcca gcgcctccct tatcgtgtcc
     2461 gcggcgaggt gctccgtcat ggaaattagc acgagatccg ccactggctc tacgcgttcc
     2521 gtgggcgtgc ccgttcgtcc aaacagcgac tggtcctccg tgcactccaa gaagtggcgt
     2581 ggtgacatct tgacgctatg atgttttggc gctatcgtac gcgcatgaag cctaagcgaa
     2641 caactgtcta cctcgagccg gacctgcaca aggcgctcaa ggtcaaagcc gccgagaccg
     2701 atcaatctgt atcggacctc ataagcggtg ccgtgcgtcg tagcctactg gaggacgcc
//
LOCUS       DASQGH010000412         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13256, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000412 DASQGH010000000
VERSION     DASQGH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13256"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1143)
                     /gene="dapA"
                     /locus_tag="VEW91_08065"
     CDS             complement(259..1143)
                     /gene="dapA"
                     /locus_tag="VEW91_08065"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYM91570.1"
                     /translation="MMRLAGIVVPVVTPLREHGVDEDATARLVDSLIRAGIHGLFVAG
                     TTGEGPLLNVEERRTLLRAVRRETKGRVPVLAGIGAASTEETRRLSQWAADDGADVVV
                     AMTPYFFAFRQSELQAHLEAVIRSSKLPVALYEIPQRTGNRLAVETVRGLMHHERLIS
                     VKDSSGDMGHFLRLLQTVRTDVTVLIGDDALILPAMAAGAAGAVSGLANVCPSVLVGL
                     YNAMSSGNLSLARQLQRQVITGQDALSVESSIPAALKAALAAQGIAVGDPKPPLAPLS
                     AEATERFCRALTKAGLLP"
     gene            complement(1140..1928)
                     /locus_tag="VEW91_08070"
     CDS             complement(1140..1928)
                     /locus_tag="VEW91_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYM91571.1"
                     /translation="MSVRLGLSSVALASIHASAKTPETIARHCRGLELGAVELRPDYL
                     REQALPPAGIARIMGAHGLRLIYATDTLLADHPQGPAEPESPLRRDFAIARSLDAEIL
                     RIFPGDLSRLGPEASTVAAAWGDFAAAEGRRLVLENIGEGPGSDPLELAQFARNARGR
                     LYVNFDVGNAARAGFDPAATLDRIRDVTVMLHLKGLRPEEAGRGDAAAEGDAIDFAAI
                     ARVLRGWDGPACLEEDAGPEPWTTLAAWVRWLRSAGFPPPGRDG"
     gene            complement(1925..>2139)
                     /locus_tag="VEW91_08075"
     CDS             complement(1925..>2139)
                     /locus_tag="VEW91_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYM91572.1"
                     /translation="GYRREFLLKFTRLAPTPLERTESLEQLRALEHGYRIRVAPTRFP
                     YAGVSVDTPSDLQAVTAILARQGRQG"
BASE COUNT          340 a          710 c          736 g          353 t
ORIGIN      
        1 cagaggatcg ggcacgtgca tctcaccgac gtcaagccgc tgccgggcgg gatgcgcgca
       61 acccaccccg gtgatgggat actctggtgg tacggggtgt tctgggggct ctggtagtta
      121 gggtacgggg agtacgcgac ggtggagttc cccggggaag gggacgggga ggcgcgcctt
      181 cgctcggcga ttgactacct cgaccgtctg cgggcagctc gcttcgccgg ctcggtgcca
      241 tgaggcgcgg ccttcgtctt acggaaggag gcccgccttc gtcagcgctc tgcagaacct
      301 ctccgttgct tccgcagaaa ggggagcgag cgggggcttt ggatctccca ccgcgatccc
      361 ttgtgcggcc agggcggctt tcagggcggc cggaatcgag ctctcgacgg aaagcgcatc
      421 ctggcccgtg atcacctgac gctgcagctg ccgggccagt gacaagttcc cgctgctcat
      481 ggcgttgtac aggccgacca gcacgctggg gcagacatta gccagtccgg agaccgcgcc
      541 ggccgcgccc gcagccatcg ccggcaggat aagcgcatcg tccccgatca ggacggtgac
      601 gtcggtcctc acggtctgga ggagcctgag gaaatgcccc atgtctccgc tgctgtcttt
      661 gacggagatc agccgttcgt ggtgcatcag tccacgcacg gtctccacgg ccagtcggtt
      721 cccggtgcgt tgaggaatct cgtagagcgc tacggggagt ttcgaagatc gtatgacggc
      781 ctccagatgg gcctggagct ccgactgccg gaaggcgaag aagtaggggg tcattgccac
      841 gacaacgtcc gcaccatcat ccgcggccca ctgggacaac ctgcgggtct cctcggtcga
      901 ggcagcgccg atccccgcca ggacggggac gcgtcctttc gtctcacgtc ggactgctcg
      961 gagcagcgtc cgacgttcct ccacgttgag cagaggaccc tccccggtgg tgccggccac
     1021 gaacaggccg tggattccgg cacggatcaa cgagtccacc agtcgagcgg tggcatcttc
     1081 gtccaccccg tgctccctca gcggggtaac cacaggtacc acgatgcctg cgagtcgcat
     1141 catccgtccc tccccggcgg cggaaatccg gcagacctga gccatcggac ccacgccgcg
     1201 agtgtggtcc agggttcggg cccggcgtcc tcttcgaggc acgcgggtcc atcccagccg
     1261 cggaggacgc gggcgatggc cgcgaagtcg atcgcgtcgc cctcagcggc ggcgtcaccg
     1321 cgtccggctt cctccggacg gaggcccttc aggtgcagca tgacggtgac gtcccgaatc
     1381 cggtccagcg tggctgcggg atcgaatccc gccctcgcgg cgttgcccac atcgaagttc
     1441 acatacagcc tgccgcgggc gttgcgcgcg aattgcgcga gttcgagtgg atcgcttccc
     1501 ggcccttcgc cgatgttttc aaggacgagc cgcctgccct cagcggcggc gaagtctccc
     1561 caggccgcgg cgacggtcga agcctccggc ccgaggcgac tcagatcccc ggggaagatc
     1621 cggaggatct ccgcatcgag ggagcgcgcg atggcgaaat ctcgccggag cggactctcc
     1681 ggctccgcgg gtccctgtgg gtggtcagcg agcagggtat cggtcgcgta gatcagacgg
     1741 aggccatgag cgcccatgat ccgggctatc ccggcgggcg ggagggcctg ttcccgcagg
     1801 taatcgggcc ggagttcaac cgcaccgagt tccagcccgc ggcaatgtct ggcgatcgtc
     1861 tcgggggtct ttgcgctcgc atggatcgat gcgagcgcga ccgaactgag gccgagccgc
     1921 acgctcaccc ctgccggcct tgccgggcca ggatggccgt gaccgcctgg agatcgctcg
     1981 gcgtgtcgac gctgacgccg gcgtacggga accgcgtcgg ggccacccgg atgcgatacc
     2041 cgtgttccaa cgcccgcaat tgctcaaggg actcggtacg ctccagcggt gttggggcga
     2101 gacgggtgaa cttcagcaag aactcacggc ggtacccgt
//
LOCUS       DASQGH010000413         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30217, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000413 DASQGH010000000
VERSION     DASQGH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30217"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VEW91_08080"
     CDS             complement(<1..740)
                     /locus_tag="VEW91_08080"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYM91573.1"
                     /translation="MPAATRPGAGVRALADRAARRARPARGGLYFVREGILGFGHNGL
                     ASAAAVTTTMVTLIALGAALVVASTVDQLARAVELKAQIVVYLRDGLRASEVAAVRAR
                     LTGLPGVTRVTYKSKDDALVAFQQRLGGRVDLTEFLSHNPLPASFELMVDPSSRLAAI
                     EAAAGALPQVERATYGAETVDRLLAVTRAVRLGGTIVSGGLALVAMIIIVNTIRLTVL
                     ARRAEIEVMRLVGATAWFIRRPFVVEGAV"
     gene            complement(724..1410)
                     /gene="ftsE"
                     /locus_tag="VEW91_08085"
     CDS             complement(724..1410)
                     /gene="ftsE"
                     /locus_tag="VEW91_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HYM91574.1"
                     /translation="MVEFQQVSKVYPNGVCALREVTLRIEPEEFVFIVGPTGTGKSTL
                     TRLIYREELPSMGHVLVDGRDVTRMPPRQVPFLRRRVGVVFQDFKLLPRRTAWENVAF
                     ALEVTGAPRAEIGNRVGEVLEVVGLAARAEAVPGELSAGEQQRVSIARALVHRPPLLV
                     ADEPTGNLDPDTSWEIVQLLSRINLAGTTVIVTSHDKPIVDVLRKRVIALDGGAVVRD
                     EARGLYAGGN"
     gene            complement(1467..2234)
                     /gene="surE"
                     /locus_tag="VEW91_08090"
     CDS             complement(1467..2234)
                     /gene="surE"
                     /locus_tag="VEW91_08090"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HYM91575.1"
                     /translation="MRVLLTNDDGLGAPGLLALARTMRKAADTFAVAPEHERSAASHA
                     ITLHKPLRAVRTKLGDTGIPAWATNGTPADCVVLGVLDLLRTPPDVVVAGINSGPNLG
                     MDLFYSGTVSGAVEATLFGISAIAVSVASFTDIHWQPAATFATHLAKEVNRNGLPRDT
                     FLNVNVPNLPAAQIRGVEITRQSTRRYVSRLEKRADPRGRDYYWLTGSPTGGEDPGTD
                     VRAVAEGRISVTPLRLDMTHDELTPMLRSWNLKLPKR"
     gene            complement(2254..2817)
                     /locus_tag="VEW91_08095"
     CDS             complement(2254..2817)
                     /locus_tag="VEW91_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HYM91576.1"
                     /translation="MSREPSPAECSIGSARIRIVKGDITALACDAIVNAANDRLWMGG
                     GVAGAIKRRGGDAIEREAVRHGPVQIGQAVATGAGRLPARYVIHAVTMGQDLATSEQA
                     IRAATQSALRLAAQLGLRSIALPALGTGVGGFPLDRAARAMLEETARHLSEVGRPPEV
                     VFALYDEPAYAVFARALRGLTEPCGTG"
     gene            complement(2849..4027)
                     /locus_tag="VEW91_08100"
     CDS             complement(2849..4027)
                     /locus_tag="VEW91_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYM91577.1"
                     /translation="MTTAPNTGPQADAGASNQDVERLLQQLRSADVTLRSDAAHGLGK
                     LADERAVPSLGEALHDSDEYVRKSAVMALRRIGGPKSMEAMRVALADRSEQVCLQAVK
                     GLGELRDAGAVEALIKVLARRERSLVQAATEALSRIGPEAVPPLMEAFKDRYLRRRIG
                     AQIWRILTEMGSRAIDPLVGVLADDNYYVRLSALTILGRIGDKRVVESILRVFLSDPR
                     LQETVVGTIGRLEERGVLVLPTGDREVVLPVEVVHSFAQQPRDAVLEVLNAAMENPSV
                     KVRRFSLKVLYQLLGEATFEHLLRYLGDDDPDVKRLVIRLLSKLRDKRVIEPLLGLLL
                     KDGGQVEEAVWDALKVLTNLHEYEELRKRVAREKAGTRPVVKTYKKKDVSPDWWRDQD
                     "
     gene            4181..>4410
                     /locus_tag="VEW91_08105"
     CDS             4181..>4410
                     /locus_tag="VEW91_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91578.1"
                     /translation="MTDTVRFAHRSEAEFAKILDFYGIRWLYEPRTFPLEWDDRGYVI
                     ESFNPDFYLPEYDLFIELTTLKQSLVTRKNRKV"
BASE COUNT          676 a         1594 c         1454 g          686 t
ORIGIN      
        1 accgccccct cgacgacaaa cggcctccgg atgaaccacg cggtcgcgcc gaccagccgc
       61 atgacctcga tctccgcgcg gcgggccagg acggtcagcc ggatcgtgtt cacgatgatg
      121 atcatcgcca ccagcgccaa cccgccgctc acgatcgtgc cccccaggcg caccgcccgg
      181 gtgacggcca ggagccgatc caccgtctcc gctccgtagg tggcccgttc gacctgcggg
      241 agcgcgccgg ccgccgcctc aatcgccgcc agacgcgagg aggggtcgac catgagctca
      301 aacgacgcgg gaagcgggtt gtgcgatagg aactccgtga ggtccaccct cccccccagc
      361 cgctgctgga aggccaccag ggcgtcgtcc ttcgacttgt aggtgacccg cgtcaccccc
      421 ggcaggccgg tgagccgcgc ccgcaccgcc gcgacctcgc tcgcccgaag gccatcccgg
      481 aggtacacga cgatctgggc tttcaattcc acggcgcggg ccagttggtc gacggtgctg
      541 gcgacgacca gggcggcccc cagcgctatc agggtgacca tggtggtggt cacggctgcg
      601 gcgctcgcca gcccgttgtg cccgaatccc aggatccctt ccctgacgaa gtacagcccc
      661 ccgcgcgccg gcctcgcccg gcgcgcggcg cgatcggcca gcgcgcgcac cccggccccg
      721 ggcctagttg ccgccggcat acagtccccg cgcctcatcc ctgaccacgg cgcccccgtc
      781 aagcgcgatg acgcgtttcc gcaggacatc gacgatgggc ttgtcgtgcg aggtcacgat
      841 gacggtggtc cccgccaggt tgatccggga gaggagctgc acgatctccc acgaggtgtc
      901 cgggtcgagg ttgcccgtgg gctcatcggc gacgaggagc ggggggcggt gcaccagtgc
      961 ccgcgcgatg ctgacccgct gctgctcccc cgccgacaac tcccccggca ccgcctcggc
     1021 tcgggcggcg aggcccacga cctccagcac ctcgccgacg cggttgccga tctccgcccg
     1081 gggggcgccg gtgacctcca gggcgaacgc gacgttctcc caggcggtcc tgcgtgggag
     1141 gagcttgaag tcctgaaaga cgacgccgac ccgtcgcctg aggaacggca cctggcgggg
     1201 cggcatccgg gtcacatcgc gcccgtccac gagcacgtgc cccatggatg ggagctcttc
     1261 ccgatagatc agtctggtga gggtggactt gccggtcccg gtcggcccga cgatgaacac
     1321 gaactcttcg ggctcgatcc gcagggtcac ctcgcggagc gcgcacacgc cgttcgggta
     1381 caccttgctc acctgctgga actcgaccat cgcgaccccc ggggcggcgc cggagggatc
     1441 ggagcgctcc gaaggcgcgt cgggggttac cgcttcggga gcttcaggtt ccatgaccgg
     1501 agcatcggtg tgagctcgtc gtgggtcatg tccagccgaa gcggcgtcac ggaaatccgg
     1561 ccctcggcca cggcccgcac gtccgtcccg gggtcctcgc cgccggtggg ggacccggtc
     1621 aaccagtagt agtcgcggcc ccggggatcg gcccgcttct caagccggct gacgtaccgc
     1681 cgggtgctct gccgggtgat ctccaccccg cggatctggg ccgccggcaa atttggcaca
     1741 ttgacgttca ggaaggtgtc ccgaggcagc ccgttccggt tgacctcctt ggccaggtgc
     1801 gtcgcgaagg ttgccgcggg ctgccagtgg atgtcggtga acgaggcgac cgagaccgcg
     1861 atcgccgaga tcccgaacag cgtcgcttcg acggctcccg agacggtccc ggagtagaag
     1921 aggtccatgc cgaggttcgg ccccgagttg atccccgcga cgaccacatc gggcggagtc
     1981 cggagcaggt cgaggacccc gagaacgacg caatccgcgg gggtcccgtt cgtcgcccac
     2041 gcgggtatcc cggtgtcacc caatttcgtc cggaccgccc gcagcggttt gtggagggtg
     2101 atcgcgtggc tcgccgcgct ccgctcatgc tcgggagcca cggcaaacgt gtcggccgcc
     2161 ttccgcatcg tgcgggcgag cgccagtaac ccgggcgcac cgagcccatc gtcgttcgtg
     2221 aggagcaccc gcatcagggg acccccaccg cgcctatccc gtcccgcacg gttctgtgag
     2281 cccgcgcagc gcgcgggcga acacggcgta cgccggctcg tcgtacagcg caaacacgac
     2341 ctccgggggg cggcccacct cggacaggtg ccgggccgtc tcctcgagca tcgcccgggc
     2401 cgcccggtcg agcggaaagc ctccgacgcc ggtcccgagc gcgggcagcg cgatcgaccg
     2461 cagccccagt tgcgcggcca accgcagggc gctctgggtg gcggcccgga tcgcctgctc
     2521 gctggtcgcg aggtcctgac ccatcgtgac cgcatggatc acgtagcggg cgggaagtcg
     2581 gccggctccg gtggcgaccg cctgtccgat ctggacgggg ccgtggcgca cggcctcgcg
     2641 ctcaatcgca tcgccgccgc ggcgcttgat cgccccggcg accccgccgc ccatccacag
     2701 acggtcgttg gccgcgttca cgatcgcatc gcacgcgagg gccgtgatat cgcccttcac
     2761 gatgcgaata cgagcgctcc cgatcgaaca ctcagcgggg gatggctcgc ggctcatccg
     2821 cgtcgcggga attcgagcgg cgtgcccctc agtcttggtc gcgccaccag tccggggaca
     2881 cgtccttctt cttataggtc ttgaccaccg gccgggtccc ggccttctcg cgggcgacgc
     2941 gcttgcgcaa ctcctcgtac tcgtgcaggt tggtcagcac tttgagcgcg tcccagacgg
     3001 cttcctcgac ctgaccgccg tccttcaaga ggaggcccaa caatggctcg atgacccgct
     3061 tgtcccgcag tttgctgagg agccggatca ccagccgctt gacgtcgggg tcgtcgtcgc
     3121 caaggtagcg gagcaggtgc tcgaacgtgg cctccccgag gagctggtag agcaccttca
     3181 gggagaaccg gcgcaccttc acgctggggt tctccattgc cgcgttgagc acctcgagga
     3241 cggcgtcgcg cggctgctgc gcaaacgagt ggacgacctc gaccggcaac accacttccc
     3301 ggtcgccggt cggcaggacc agcacccccc gctcctccag ccgtccgatc gttcccacga
     3361 ccgtctcctg gagccgggga tcgctgagaa acacccgcag gatcgactcg acgacgcgct
     3421 tgtcgccgat ccgcccaaga atggtcagcg cgctcaggcg gacgtagtag ttgtcgtcgg
     3481 caagcacccc gacgaggggg tcgatggcac gggatcccat ctcggtgagg atgcgccaga
     3541 tctgcgcgcc gatccgcctc cggaggtacc gatctttgaa cgcctccatg agggggggca
     3601 ccgcctcggg gccgatgcgc gacaaggcct ccgtcgccgc ctggacgaga gagcgctccc
     3661 ggcgcgcgag gaccttgatc agcgcttcca ccgcgccggc gtcgcgcagt tcgccgagtc
     3721 ccttgaccgc ctgcaggcac acctgctcgg accggtcggc gagcgcgacc cgcatcgcct
     3781 ccatggactt ggggccgccg atccggcgca atgccatcac ggcgcttttc cggacatact
     3841 cgtccgaatc gtgcagcgcc tcgcccaggg aggggaccgc gcgttcgtcc gccagtttcc
     3901 ccaaaccgtg ggccgcgtcg ctgcgcagcg tcacatcggc gcttcggagc tgctgcagga
     3961 gccgctcaac atcctggttg gaggccccgg catccgcctg aggccccgtg tttggcgccg
     4021 tggtcatcac tcgtgctcct ttgtatatag tatatccgtt cggcgccccg gggaggcttt
     4081 ccttgattat ttctacattg gccgctatac taactcccag taggccgtta gcctgcccgg
     4141 cggagggatg aacgggaccg ttgaacgccc caggggtagc gtgacggata ccgtccggtt
     4201 cgcccacagg agcgaagcgg agtttgcgaa gatcctcgac ttctacggga tccgatggct
     4261 atacgagccc cgcaccttcc ccctcgagtg ggacgaccgc ggctacgtga tcgagagctt
     4321 caacccggac ttctacctcc ccgagtacga cctgttcatt gaactcacca cgctgaagca
     4381 gagccttgtg acccgaaaga accgcaaggt
//
LOCUS       DASQGH010000414         7709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10210, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000414 DASQGH010000000
VERSION     DASQGH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10210"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(559..945)
                     /locus_tag="VEW91_08110"
     CDS             complement(559..945)
                     /locus_tag="VEW91_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91579.1"
                     /translation="MVVGPRVEEAPLPSVHVEIPEEGITIIPVPRVIEMFSVSERELE
                     QIAAASGSFAFHSVFFGITFGASMAFIVSLLSAELPNRMYALVWALFVLTLATIYFGV
                     QASRTYREAGKGVRTIKTESRRVPPE"
     gene            complement(1056..3368)
                     /locus_tag="VEW91_08115"
     CDS             complement(1056..3368)
                     /locus_tag="VEW91_08115"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYM91580.1"
                     /translation="MMHPTIAWIGRPVKRREDLRFLQGQARYVDDLTLPGMTHLVVVR
                     SAAAHARLRAVNLEPARRSPGVVAVVTGRDLAGQVHPLPVNVPEPASVAQIAHPVLAG
                     DVVRYVGEPIAGVLAESRAAAEDAAHQVDVQYDPLPAVTNVRDALAGKVRLHDSVEDN
                     VLIRLSRGSGDVEAAFRSAERVVRERFHLPRIAAAPMEPRGAVAAHDPGPDLLTVWLS
                     AQGPYRPRAQISQILARPEGKIRVVVPDVGGAFGSKGTVAPEAVLAAWLAIKTGRPVK
                     WGEDRRENLMASYQGRGIDAEVEVAVDHRGRIAALRATVIADVGAYLYQPTTVPPSRV
                     GDLLTGAYDIPVAKVDVRGVATNKVPTGPYRGAGRPEAAYIVERMVDRVAGELGLDPV
                     EVRRRNLIPPDRFPYRTPLGSVYDSGDYGRALDRACVLAEYARWREEQRRARSHGRLV
                     GIGVATYIEPAGSQVWESAAVSVTPQGRVVIRTGSTAHGQGHDTTFAQIAAEALQIPI
                     EAISIEQGDSARLTKGMGTFGSRSTTIGGSALWNVLQTVKTKMVKIAAHLLEAAEGDI
                     RWGEGRLSVQGAPARALTFQEVAAAAVEPARLPPGIEPGLEASGTFSLAGMVYPSGAY
                     IAVVEVEPPTGLVRILRLVAVDDAGRIINPLLAEGQVIGATVQGLGQVLAEEVVYDDG
                     GQLLTGTFADYALLRAAHVPHIESEFLETPSPLNPLGAKGIGEAGTVGAPPALANAVI
                     DALAPLGVINIDLPLTQEKLWRLTRTARTP"
     gene            complement(3427..4491)
                     /locus_tag="VEW91_08120"
     CDS             complement(3427..4491)
                     /locus_tag="VEW91_08120"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYM91581.1"
                     /translation="MIYRTVIQPILFGIEPETSHDAVLTILGAVGPVWSRLSGPPHDP
                     RLATVVCGITFPAPLGLAGGFDKGARALWAWPALGFGFAEVGTVTARPQPGNPRPRLF
                     RLPEDQALINRLGFNSPGADAVAARLTALRRRAYPIPLGVNIGRSAAVSNEAAPDDYA
                     YSLDRLHAGADFVVANLSSPNTPGLRALQRRQAIVPLLDRLAERNRALGEKPLFVKVA
                     PDLTDEELEDIVSALEGRAHGLVATNTTTRRNGLRSPARDEAGGVSGRPLRDLTTRVI
                     RTLWRLTRGRVPIIGVGGVFTPEDAYEKIKAGASLIELYTGFVYRGPGTPRRILDGLR
                     ALLDRDGLGTIAQAVGVDTR"
     gene            complement(4571..5272)
                     /locus_tag="VEW91_08125"
     CDS             complement(4571..5272)
                     /locus_tag="VEW91_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYM91582.1"
                     /translation="MPEPPPILLIPGLLCSPRLYGEQLPALWRFGPLVIADHTRDDSM
                     AAIARRILAAAPPRFALIGLSMGGYIAFEIMRKAPDRVARLALLDTSARADTPEQSER
                     RRAQIALAQSGRLAEVLDQLFPLWVHRARRNDESLRRTVRLMAEETGPQAFTRQQAAI
                     MTRPDSRPDLAAIRCPTLVLVGDGDESTPPDRAAEIANGIASARLVTVPECGHLSTLE
                     RPQHVTQALIEWMEA"
     gene            5454..6815
                     /gene="allB"
                     /locus_tag="VEW91_08130"
     CDS             5454..6815
                     /gene="allB"
                     /locus_tag="VEW91_08130"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HYM91583.1"
                     /translation="MPVDLIVKNGTIVTPLSSRRGHVLIRDEKIVDIMVGDALPQSRE
                     VIDATGLHVLPGLIDPHVHFRVPGLDYKEDFETGSQAAACGGITTVLDMPNVVPPTSN
                     IEGFEAKVACAQGRSYVDYGLYAVIVEGNMDQILPLADAGVVGYKIFLGETVGTLPAP
                     PDGEIIDAWRLMARTGLRCGVHAEDNGIILYLRKKLQAQGRKDPLAHLESRPSVAEAE
                     AISRAILFAREAGSRLMILHMSAAEGVELVRRGKDSGVDVMGETAPHYLLLEGEDMVQ
                     MGLGTLMKMNPPVRSREHGEALWRGLLDGTIEVIGTDHSPHTPEEKMADTPMGDIWRA
                     MAGLAGVETGVPLMLTQVNAGRLSLNRYVKVQAEGPARAWNLWPRKGNLGRGGDGDLT
                     VVDLQQEGVVDRSRLHSKSKVTPFHGFRVKGMPVYTIVRGHVVMKHGELYGNPIGQLL
                     RPG"
     gene            6845..7303
                     /locus_tag="VEW91_08135"
     CDS             6845..7303
                     /locus_tag="VEW91_08135"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYM91584.1"
                     /translation="MKFEKEITVSVRPERVWAFLWDVDRVTRCLPGCKEARTIVPHER
                     YEAVIGERVGPFKVQFPLAIQVLEARAPRRLRAEAIGRDAAMGSALRVTLDLLLEGQG
                     AGSRLLIQSDVGILGKLGTLGHGIIQQKANGIMTQFAESMRKELEAEGQA"
     gene            complement(7357..>7709)
                     /locus_tag="VEW91_08140"
     CDS             complement(7357..>7709)
                     /locus_tag="VEW91_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HYM91585.1"
                     /translation="QKVLEVKPDYVVFNGVADQYQEHPLVVKAGQRIRLYVLNAGPTL
                     FSAFHVIGWIFDKVYVDGNPGNVLQGVQTYTIPPGGGATFELTITQPGLYPFVTHAFA
                     FTGKGAVGVIKVVK"
BASE COUNT         1239 a         2574 c         2716 g         1180 t
ORIGIN      
        1 aacccgtagg ccaccttggc aaccgggatg atggtgcacc cattctcgtg aatgggctcc
       61 ccaaacaccg tcttcacggt taccctctcg tcgaatcgac cgaggtgggt caggacttcc
      121 tcgagcatcg cacatcaccc cctcggagcg cgaccccgcg ccccatcaat gacactatac
      181 gtcaggtctc ctatctaatc cacaatcctc gaaattaggt cggatgttct ggatggaata
      241 cggcgacgag attagatcgc cggaatttta cggagcgatc cgatgtttcg ccatccgttt
      301 tccaagagcg aacaggcctg gaatcgaagc cagaagacag attgttacca tagtagtagg
      361 gggcggcggg agcggagggg agccatggtc cgatcgaacg aaagttaggt cgagagttgg
      421 tgttgatgga cgagcagcaa aaggggcccg tgtatgcgac cgctcggacg acaagctgga
      481 gagtgggaag ccgctccacc gacaccaggg tttccactgc agaggcggcc ggctggcgga
      541 agcggcgggc gagcggggct actctggggg gaccctccgg ctctcggtct tgatcgtgcg
      601 aacccccttc cccgcttcac ggtacgtgcg gctggcctgg actccgaagt agatggtcgc
      661 cagggtcagc acgaagaggg cccagacgag cgcgtacatc ctgttgggca gttccgcgct
      721 caacagggag acgataaacg ccatcgaggc gccgaacgtg atcccgaaga acaccgaatg
      781 aaaggcgaac gacccggagg ccgcggcgat ctgctcgagc tcccgctcgc tcacactgaa
      841 catctcgatg acgcgtggca cgggaatgat cgtgatgcct tcttcgggga tttcgacgtg
      901 cacgctcggg agcggtgctt cttcgactcg gggacccacc accatcgatt gttcctccct
      961 tgcgggaatt accgggctat gctacaacgc ggcgttggaa gtctacacac tggtatgccc
     1021 ttccccctga tgctctagac ccgcgtctgg cgtccctacg gggtccgcgc ggtgcgggtg
     1081 agccgccaga gcttctcctg ggtgagcggg aggtcgatat tgatcacgcc gagcggcgcg
     1141 agcgcgtcga tgacggcgtt ggccagagcc ggcggcgccc cgacggtgcc ggcttccccg
     1201 atccccttcg cgcccagcgg gttcaggggg gacggggttt cgagaaactc gctttctatg
     1261 tgggggacat gggccgcccg gaggagggcg tagtcggcga atgtgccggt caggagctgg
     1321 cctccatcgt cgtacacgac ctcctcggca agcacctgcc cgaggccctg caccgtcgcg
     1381 ccgatcacct gcccctcggc gagcagcggg ttgatgatcc ggccggcatc atccaccgcc
     1441 acaagcctta ggatgcgcac gaggccggtc gggggctcca cctccaccac cgcgatgtag
     1501 gcgccggacg ggtacaccat gcctgccagg gagaaggtgc ccgacgcctc gagccccggc
     1561 tcgatccctg ggggaaggcg ggcgggctcg acggcggcgg cggcgacctc ctgaaaggtg
     1621 agcgcgcggg cgggtgcccc ctgcacggag aggcggccct cgccccagcg gatatcacct
     1681 tctgcggcct cgaggaggtg cgcggcgatc ttcaccatct tggtcttgac cgtctgaagg
     1741 acgttccaca gcgccgatcc gccgatcgtg gtggaccggc tgccaaaggt gcccatgccc
     1801 ttggttagcc gcgccgagtc gccttgctcg atcgagatcg cttcgatcgg gatctggagc
     1861 gcctcagcgg cgatctgggc gaaggtggtg tcgtgcccct gcccgtgggc ggtcgatccg
     1921 gtgcggatca cgacccgtcc ctggggcgtg acggagaccg cggcgctttc ccacacctgg
     1981 ctgcccgcgg gctcgatgta cgtcgccacc ccgattccga cgagcctccc atgggaccgt
     2041 gcccggcgct gctcctcgcg ccaccgcgcg tactcggcca gcacgcacgc tcgatccagc
     2101 gcccgcccgt agtcgccgga gtcgtagacg gagcccaggg gggtgcggta gggaaagcgg
     2161 tctggaggga tcagattgcg gcgccggacc tccacggggt cgagccccaa ctcgccggcg
     2221 acgcggtcca ccatgcgctc cacgatgtac gcggcctccg gccggccggc gccccggtag
     2281 ggtcccgtgg gcaccttgtt ggtcgcaacc cccctcacgt cgaccttcgc caccgggatg
     2341 tcgtaggcac ccgtcaacag atccccgacc ctcgaaggtg ggaccgtggt gggttggtac
     2401 aggtacgctc ctacgtcggc gatcaccgtg gcccggagcg ccgcgatcct cccgcggtgg
     2461 tcgaccgcca cctccacctc ggcatcgata ccccgcccct gataggaggc catgaggttc
     2521 tcgcgccggt cctcgcccca cttgaccggc cggccggtct tgatggcgag ccaggccgcg
     2581 agcacggctt caggggccac ggtgcccttg ctgccgaagg cgccccccac gtccgggacc
     2641 acgacccgga tcttgccttc gggtctggcg aggatctggc tgatctgggc gcggggtcga
     2701 taggggccct gggccgagag ccagaccgtc aacagatcgg ggccgggatc gtgtgcggcc
     2761 accgcccccc tcggttccat gggcgcggcc gcgatacggg gaagatggaa ccgctcccgc
     2821 accacgcgct cggcgctccg gaacgcagct tcgacatcgc cgctccccct cgacaagcgg
     2881 atcaggacgt tgtcctccac cgagtcgtgg aggcgcacct ttccggcgag ggcgtcgcgg
     2941 acgttcgtga ccgccggcag aggatcgtac tgcacatcga cctggtgagc agcgtcctcg
     3001 gccgccgcac ggctctcggc cagcaccccg gcgatcggct cgccgacgta gcgcacgaca
     3061 tcccccgcaa ggacagggtg agcgatctgc gcgacgctcg cgggctcggg cacgttgacg
     3121 ggaagcggat gcacctggcc ggcgagatcc cgcccggtca cgaccgccac cacgccgggg
     3181 gagcggcggg ccggctcgag gttgactgcg cggaggcggg cgtgggcggc cgcgctgcgc
     3241 acgaccacca ggtgggtcat ccccggcagg gtgaggtcat cgacatacct ggcctggccc
     3301 tgcaggaacc gcaggtcctc gcgccgtttg acgggacggc cgatccacgc gatggtgggg
     3361 tgcatcatgg cggatgaatc acgatccata cggtcccccc cgatgccagc acgcggtgcc
     3421 cgacccttac cgggtgtcca cgcccacggc ctgcgcgatg gtcccgagcc cgtcccggtc
     3481 cagcagggcg cgcagcccat cgagaatccg ccgcggggtg ccgggtcccc tgtacacgaa
     3541 tcccgtgtag agctcgatca gcgaggcccc ggccttgatc ttctcgtacg cgtcctccgg
     3601 ggtgaacacc ccaccgacgc cgatgatcgg cacgcggccg cgggtcagcc gccacagcgt
     3661 gcggatcacc cgggtggtga ggtcccggag cggtcgcccg ctgaccccgc cggcctcgtc
     3721 gcgcgcgggg ctgcggaggc cgttccggcg ggtggtggtg ttggtcgcca ccagcccgtg
     3781 cgccctcccc tcgagcgcgg agacgatgtc ttccagttct tcgtcggtga ggtcgggggc
     3841 aaccttcacg aagaggggct tctcccccag tgcgcggttc cgctcggcca ggcggtcgag
     3901 cagcgggacg atcgcctgac gccgctggag cgcccggagc cctggggtgt tgggcgagga
     3961 gaggttggcg accacgaagt ccgcacccgc gtgcagccgg tcgagcgagt aggcgtagtc
     4021 atcgggggcc gcctcgttgc tgaccgcggc gctgcgcccg atgttgacgc cgagcgggat
     4081 cggataggcg cgccggcgga gagcggtgag ccgggcggcg accgcgtcgg ccccgggaga
     4141 gttgaacccg aggcggttga tcagcgcctg gtcctccggg aggcggaaca gacgcggccg
     4201 ggggttcccc ggctgcggcc gtgccgtcac ggtgccgacc tccgcgaacc cgaacccgag
     4261 ggccggccac gcccacagcg cccttgcccc cttgtcgaag ccgccggcga gccccagggg
     4321 cgccggaaac gtgatcccgc acaccacggt cgcgaggcgc gggtcgtgcg gcgggccact
     4381 gaggcgcgac cagacaggcc cgacggcccc gagaatcgtg aggaccgcgt cgtggctcgt
     4441 ctccggttcg atcccgaaca ggatcggctg gatcaccgtg cggtagatca cccgagcgct
     4501 cctctcatgc agacatggcg ctgcgcccca ttgtgctgac gtgcctcgga ctcggcggcc
     4561 tccgccggga tcaggcctcc atccactcga tcagcgcctg cgtgacgtgc tgcggccgct
     4621 cgagcgtgga caggtgacca cactcgggca cggtgacgag gcgcgcgctt gcgatgccgt
     4681 tcgcgatctc agccgccctg tcgggcggcg tagactcgtc gccgtcgccg accaggacca
     4741 gggtcgggca gcggatcgcg gcgaggtccg gccgcgagtc gggacgcgtc atgatcgccg
     4801 cctgctggcg agtgaacgct tgggggccgg tctcctccgc catgagccgc acggtccgcc
     4861 gtagggactc gtcgttccgg cgagcgcgat ggacccagag gggaaacagc tgatcgagca
     4921 cctccgcgag acgcccgctt tgggcgagcg cgatctgggc gcggcgacgc tcgctctgtt
     4981 ccggtgtgtc cgcgcgcgcc gaggtgtcga ggagcgccag ccgggccacc cgatcgggcg
     5041 ccttgcgcat gatctcgaat gcgatgtagc cgcccatcga cagcccgata agggcgaatc
     5101 ggggcggcgc cgcggcgagg atgcggcgcg cgatggccgc catgctgtcg tcgcgggtat
     5161 ggtcggcgat gacgagcgga ccgaatcgcc agagtgccgg aagctgctcc ccgtagaggc
     5221 gcggcgagca taggaggccg gggatgagga ggatcggtgg cggttcgggc atctgggttg
     5281 ctcctgaatc aggacatcac ccggtgcggt taattatacc gtacggtgcg gatccggacg
     5341 cgcgaggtcc cgggggcccg gcgtcggccg tctccaatgg gggcaatggt ggccgccgcg
     5401 cggtcgttgt gctatgtata gcagtgggga agccgtcggc ggaggcggcc ggcatgccgg
     5461 tggatctgat cgtcaagaac ggcaccatcg tgaccccgtt gtccagccgc cgggggcacg
     5521 tcctcatccg cgacgaaaag atcgtcgaca tcatggtcgg tgatgccctc ccccagtccc
     5581 gcgaggtcat cgacgcaacc ggcctccacg tcctgccggg cctcatcgat ccccatgttc
     5641 acttccgtgt gccggggctc gactacaagg aggacttcga gacggggtcg caggccgcgg
     5701 cgtgcggcgg aatcacgacg gtcctcgaca tgcccaacgt cgtcccgccg acctcgaaca
     5761 tcgaggggtt cgaggcgaag gtcgcctgcg cgcagggcag atcgtacgtg gattacgggc
     5821 tctacgcggt gatcgtcgag gggaacatgg accagatcct gccgctcgcg gacgcggggg
     5881 tggtcgggta caagatcttc ctcggcgaga cggtgggcac cctccctgca cctccggacg
     5941 gcgagatcat cgacgcctgg cggctgatgg cccggaccgg cctccggtgc ggcgtgcacg
     6001 ccgaggacaa cggcatcatc ctctacctcc gcaagaagct gcaggcccag gggcggaagg
     6061 atccgctcgc gcacctcgaa tcccggccct cggtggccga ggcggaggcg atctcccgcg
     6121 cgatcctgtt tgcccgggag gccgggagcc ggttgatgat cctgcacatg agcgccgccg
     6181 aaggggtgga gctggtccgg cggggcaagg acagcggggt cgacgtgatg ggggagaccg
     6241 cgccgcacta cctgctcctg gagggcgagg acatggtcca gatgggcctc ggcacgctga
     6301 tgaagatgaa cccgccggtt cgctccaggg agcacgggga agcgctctgg cgcggcctgc
     6361 tcgacggcac gatcgaggtg atcggcacgg accactcgcc gcacaccccc gaggagaaga
     6421 tggcggacac cccgatgggg gacatctggc gggcgatggc cgggttggcc ggggtggaga
     6481 ccggcgtccc gctcatgctc acccaggtga acgccggccg cctcagcctc aaccggtacg
     6541 tgaaggtgca ggccgaaggc ccggcgcggg cgtggaatct gtggccgcgc aagggcaatc
     6601 tcggccgcgg gggcgatggc gacctgaccg tcgtcgacct gcagcaagag ggtgtcgtcg
     6661 accggagcag gttgcacagc aagagcaagg tgaccccatt ccacggcttc cgggtcaagg
     6721 gcatgcccgt ctacaccatc gtccgcggac acgtggtgat gaagcacggg gagctctatg
     6781 gaaatccgat cggacagttg ctccgccccg gctagggcga ccacatcagg cacaggaggc
     6841 gcggatgaag ttcgaaaagg agatcacggt ctcggtccgg cccgagcggg tctgggcgtt
     6901 cctctgggac gtcgaccgcg tgacccgctg cctgcctggc tgcaaggagg ctcggacgat
     6961 cgtcccgcac gagcggtacg aggccgtgat cggcgagcgg gtggggccgt tcaaggtgca
     7021 gtttcccctg gcgatccagg tgctcgaggc ccgggcaccc cggcgcctca gagcggaggc
     7081 gatcggccgg gacgccgcga tgggcagcgc gctccgggtg accctggacc tcctcctcga
     7141 agggcagggc gcgggctccc ggctcctcat ccagtcggac gtcgggatcc tgggaaagct
     7201 cggcacgctg gggcacggca tcatccagca aaaggccaac ggcatcatga cccagtttgc
     7261 cgagtcgatg cgcaaagaac tcgaagcgga gggacaggcc tagccaggtc cggggtcagg
     7321 cagcggcgga gcccacgcga tcgtcaggac gtgctgctac ttcacgactt tgataacgcc
     7381 cactgcgccc ttgccggtga acgcgaaggc atgtgtgacg aacgggtaca atccgggctg
     7441 cgtaatcgtc aactcaaatg tcgcgccccc gcccggggga atcgtgtagg tctggacccc
     7501 ctggagcacg tttcctgggt tgccgtcgac gtagaccttg tcgaagatcc agccgatcac
     7561 atggaaggcc gagaacaggg tcgggcccgc gttcaggacg tacagccgga tccgctgccc
     7621 cgccttcacc accagggggt gctcctggta ttgatccgcg acgccgttaa agaccacgta
     7681 gtccggcttg acctcgagga ccttctgga
//
LOCUS       DASQGH010000415         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21509, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000415 DASQGH010000000
VERSION     DASQGH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21509"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..666
                     /locus_tag="VEW91_08145"
     CDS             340..666
                     /locus_tag="VEW91_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91586.1"
                     /translation="MKPLAGHWVYWVVIGLKTLAIVTILRPIFVRHISKAVTVFMQAA
                     PGVLSQSRHRPSYTYFSGFHVLNFSDVQWSLLEGIAVYAVALTIQVAAARLLTSGRAA
                     QNRAAF"
     gene            complement(677..1147)
                     /locus_tag="VEW91_08150"
     CDS             complement(677..1147)
                     /locus_tag="VEW91_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYM91587.1"
                     /translation="MEDSISRRGFIKQAATGIAAGAVLPVSAQAQGPAGTSLYARLGG
                     YDALAAVTDDFVGRLVSDRQLARFFAGHSTNSLNRIRQLVVDQLCQATGGPCVYIGRD
                     MKTAHKGLGISESDWQVTARHLTATFDKFKVPERERTEVLALLTSLKGDIVEKA"
     gene            1378..1563
                     /locus_tag="VEW91_08155"
     CDS             1378..1563
                     /locus_tag="VEW91_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91588.1"
                     /translation="MGLIVLIVFLAVAAAYYFLGTPLLDYIHKFTKEFTHTFPLSTFF
                     LPYPAVRAGRSRPKGRR"
     gene            1653..2417
                     /locus_tag="VEW91_08160"
     CDS             1653..2417
                     /locus_tag="VEW91_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HYM91589.1"
                     /translation="MQECAQCGHELEPFFDSTCESPVAWLCQACGRVALEQDARSLSS
                     AARRELAALQAFIAPDARGDRAPRTSMEERTGLGRAHARAHAILEGARSVPIDIERIA
                     EERGYPVRERSLPPEERGTIARDGDHTVIVINRDRDWSLAERRWVIAEELGHAVLEHS
                     TLVASAAPGRPLAIPEPRRRAEEREAKRFAAEVLMPEEKVRGRFAELAPRIYRSLGLR
                     QRQAETDEVVAALARMFAVSPLAMRVRLEELNLIQQ"
     gene            complement(2433..2672)
                     /locus_tag="VEW91_08165"
     CDS             complement(2433..2672)
                     /locus_tag="VEW91_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91590.1"
                     /translation="MAFSQEWPEGYYEIGSSGSQPAPSRPVRFVCGACPALHKVLNLS
                     PDDSSDREKLMVDAATFGVIRMAGGGQVTLLEWNP"
     gene            complement(2969..4321)
                     /locus_tag="VEW91_08170"
     CDS             complement(2969..4321)
                     /locus_tag="VEW91_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYM91591.1"
                     /translation="MSLLRAASVRWLVVALAVVILAVPLGPARPAAAADHSLVFAALH
                     VLDEEYVDPVEPVPLLNAAVGALRKTANQSAAVLPDIPGGTSRADAEVAFAATFARAV
                     QMSSIPETQLAYAATAGMLASLHDSHTNFLDPKQYEESRLQLQGRPGFSGIGVIITSR
                     KDPGGDGWIFIEEVFPGSPAEAAGLKRFDRIAQVDGRPLKNATSQDASQMIRGPAGST
                     VTLTLLRGEQTLTIAVVRASIQQRPVEVRFIQPGVAYVKLFEFSRGAGSSLRSSLLGL
                     GLHEPVRSVVLDLRSNPGGLIHEAVAVGSLFLHSRTVLSRITDRGHAPNVLQTSGVPL
                     FPQTPLVILVDGGSASASEILAGAFKDLRRATIVGEKTAGALGGSVTIRLPEGAGMQV
                     TVERITTPMGTLVEGVGIPPDVPVTLSINDMLRGEDTQLEAALRVVGALGIQRPARAA
                     "
BASE COUNT          730 a         1561 c         1464 g          693 t
ORIGIN      
        1 caaaccgcag ttcctcttcc cggcgctccc ctccgcgaat cgacaacccg agatagacca
       61 tcaggggccc cccgccgcgt gccgtccatt gctagcatac gacaagaatc agggaagggc
      121 atccggggct acgtggagga agatcgggct tttcttgacg cctgcctcgg gctgatccct
      181 ccccccggtc acggtcgagg acgcgctcca cagcatcgcc gtgctggacg ccgcgtatgc
      241 cagcatcgaa agcggcgggc aggtcatccc cgtccagccc cgtgacccgg caggtctagc
      301 ccctggaggt ttggggcaca caccgctacg gtgatgccga tgaaacccct ggcaggacac
      361 tgggtctact gggtcgtgat cgggttgaag accctcgcga tcgtgacgat cctcaggccg
      421 atcttcgtcc ggcacatatc caaagccgtg acggtgttca tgcaggccgc gccaggcgtc
      481 ctgagccagt cgaggcaccg gccgtcctac acgtatttct ccggattcca cgtgctcaat
      541 ttctcggacg tgcagtggag cctgctcgag ggcatcgcgg tgtacgcggt ggccctgacg
      601 atccaggtcg ccgccgcccg actgctgacc tccgggcggg ccgcccagaa ccgcgcagcg
      661 ttctaggggc cgcgggtcag gccttctcca cgatatcgcc cttcaggctt gtaagaagcg
      721 ccaggacttc cgttcgctct cgctccggaa ccttgaactt gtcgaaggtg gccgtcaggt
      781 gccgggcggt gacctgccaa tcggactcgc tgatgcccag gcccttgtgg gcggtcttca
      841 tgtcgcggcc gatgtatacg cacggcccgc ccgtggcctg gcacaattgg tcgaccacca
      901 gctggcggat ccggttcagc gagttcgtgc tgtgcccggc gaagaacctg gcgagctggc
      961 ggtcgctcac gaggcgtccg acgaagtcat cggtcacggc ggcgagggca tcgtacccgc
     1021 cgagccgtgc gtacagcgag gtccccgcgg gcccctgcgc ctgcgctgac accggcagca
     1081 ccgcgccggc cgcaatgccg gtcgccgcct gcttgatgaa tccgcgccgg gaaatgctgt
     1141 cctccatgtc ctctcctcct tcggccggat gggccggccc caattgccag ccatttctcc
     1201 cacaacggga tggttcctcc cccggcaggc gctcgggaca cgcgaccgca ccacccaggc
     1261 aggcggccgg gagggctcgc cggcgatgtc cccccttgag cctgctgtgg gcactcctgt
     1321 tagtgagacg ccgcacgacg ggcgcctggc gccaatgcaa gatcggaggt gatcggtgtg
     1381 ggcctcatcg tgctcatcgt cttcctggca gttgccgcgg cgtattactt cctcggcacg
     1441 cctttgctcg attacatcca caagtttacg aaggagttca cgcacacgtt tccgctgtcg
     1501 acgttcttcc tcccgtaccc ggccgttcgc gcgggccggt ctcgccccaa aggccggcgc
     1561 tgatcgcatt ctaagcaggg cggcctcgtg cacgggggat gcatcgaggc cgggtgcgcc
     1621 cggcttcccc tgtgtggtag aataagggtc ctatgcagga gtgcgcccag tgcgggcacg
     1681 agcttgagcc gttcttcgac tcgacctgcg agtcgcccgt ggcgtggctg tgccaggcct
     1741 gcggccgcgt cgccctcgag caggatgccc gatcgctttc ctctgccgcg cgacgcgagc
     1801 tggcggccct ccaggcgttc atcgccccgg atgcgcgggg cgaccgcgca ccacgtacgt
     1861 caatggaaga gcgcaccggg cttgggcggg cccacgcgcg ggcgcacgcg atcctggaag
     1921 gggcccgaag cgttcccatc gatatcgagc ggattgccga ggaacggggg taccctgtgc
     1981 gcgagcggtc gctgccaccg gaggaacgcg gcacgatcgc ccgggacggc gatcacacgg
     2041 tcatcgtgat caaccgcgac cgggactggt ccctggccga gcggcgctgg gtgatcgcag
     2101 aggagctcgg ccacgccgtt ctcgagcaca gcacgctggt ggccagcgcg gcccccggac
     2161 gccccctcgc gatccctgaa ccacggcgcc gggccgaaga gcgggaagcc aagcgattcg
     2221 ccgccgaagt gctgatgccc gaggaaaagg tccgagggcg cttcgccgag ttggcgccca
     2281 gaatctaccg gtccctgggc cttcggcagc gccaagccga gaccgacgag gtcgtcgccg
     2341 cgctggctcg gatgttcgcc gtgagcccgc tggcgatgcg cgtgcggctc gaagagctga
     2401 acctcatcca gcagtaggtc cccgagagac gcctagggat tccactccag cagggtgacc
     2461 tggccgcccc cggccatcct gatgaccccg aaggttgcgg cgtcgaccat cagcttttcc
     2521 cggtcgctcg aatcgtcggg ggagaggttg agcaccttgt ggagcgccgg gcacgccccg
     2581 cacacgaagc ggaccggccg cgagggcgcg ggctgcgacc cggacgagcc gatctcgtag
     2641 tagccctcgg gccattcctg tgagaacgcc atgtgcaggc ccgagaaccg ccggggtgag
     2701 gattgcgggg aaggcgatcc cgccacgaaa ctgtcaacgt cgggcagcca aacggggcgg
     2761 agcctcacca agatcgcagg ggcggcggtc gtctgccgtc gctctgcttg gacagcgaca
     2821 aaccggacgg agacgcgcca gccaagcgcc ccggcgccga tcgcagcagc ccccaaaagc
     2881 acgagggcga tgcgtagagc cctgggccac tgcccgcgtc tagccacctg gcccccgcct
     2941 ccaccccggc ctgaggatgc ggcctccgct acgccgcccg agccggcctc tggatgccca
     3001 gcgctccgac gacgcgaagc gcggcctcca gttgcgtgtc ctcgccccgc agcatgtcgt
     3061 tgatgctcag cgtcaccggc acgtctggcg ggatgccgac cccctcaaca agcgtgccca
     3121 tcggggtcgt aatccgctcg accgtgacct gcatcccggc cccctcgggc aggcggatcg
     3181 tcacggaacc gccgagggca cccgccgtct tctccccgac gatggtggcg cggcgaaggt
     3241 ccttgaacgc ccccgccagg atctccgagg ccgatgcgct gcccccatcg acgaggatca
     3301 ccagcggcgt ctggggaaac agcgggaccc cgctcgtctg caggacgttg ggcgcgtgac
     3361 cgcgatcggt gatcctcgac aacacggtcc tggaatgcag gaacaagctg ccgaccgcga
     3421 cggcctcgtg gatcagcccg cccgggttcg accgaagatc gagcacgacc gagcggacgg
     3481 gctcatgaag cccgagcccc agaagcgagc tccggaggct cgatccggcc ccccgcgaga
     3541 actcgaagag cttcacgtag gcgaccccag gctggatgaa cctcacctcc accgggcgct
     3601 gctggatcga agcgcgcacc acagcgatcg tgagcgtctg ctctccccgc agaagcgtca
     3661 acgtcaccgt ggacccggcc ggcccgcgga tcatctggct cgcgtcctgg ctggtcgcgt
     3721 tcttgagagg ccgcccgtcg acctgagcga tccggtcaaa ccgcttgagg cccgccgctt
     3781 cggccgggga ccccgggaag acttcctcga tgaaaatcca cccatctccg ccgggatcct
     3841 tgcgcgaggt gatgatcacg ccgatcccgc tgaacccggg tcggccctgg agttgcaggc
     3901 ggctctcctc gtactgcttg ggatcgagga aattggtgtg gctgtcgtgg agcgaggcca
     3961 gcatccccgc ggtggcggcg taggcaagct gggtctcggg gatcgagctc atctgcaccg
     4021 cgcgcgcgaa cgtagcggca aacgccacct cggcgtcggc tcgggacgtc ccgcccggga
     4081 tgtccggcag caccgccgcg ctctgattcg cggtcttccg cagagcgccg accgcggcgt
     4141 tgagcagcgg gaccggctcg accgggtcca cgtactcctc atcaagaacg tgcagggctg
     4201 cgaagaccag cgagtggtct gcggcagcgg caggccgggc cgggccgagc gggaccgcca
     4261 ggatgaccac ggcgagcgcc accacaagcc acctgacgga ggctgcgcgc aagagggaca
     4321 tatctcacgc tcctgtgtga tcgcaatgcg gggggtagaa ccggtacccc tccggaacct
     4381 ctccccgatt atacacgtat atacccgaaa tcaagttcca tagcgtctct gaacgccgaa
     4441 aaattccg
//
LOCUS       DASQGH010000416         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10223, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000416 DASQGH010000000
VERSION     DASQGH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10223"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..645)
                     /locus_tag="VEW91_08175"
     CDS             complement(355..645)
                     /locus_tag="VEW91_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HYM91592.1"
                     /translation="MVTIKAIWQGQVIAESDRTLEVGGYRYFPRETVRMDWLRPAPKT
                     QGDLACPHGVQFYDIVVDDARSLRAAWSYEAPRASMKQVDHWIGFWEDVEIE"
     gene            complement(718..1536)
                     /locus_tag="VEW91_08180"
     CDS             complement(718..1536)
                     /locus_tag="VEW91_08180"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYM91593.1"
                     /translation="MPGRAATVETAPPATHAVTACARSPADGPQDYAVRDRTADVARW
                     RTMPLLFKAIANLLFWMSFFAAVLFVPAGTGNWWRGWVFLGVLGAGMVYAVASLLPVH
                     RDLVEERLKLPIQREQPLTDKIVVTLFYAAFYGLVAFIPLDVFRFDLLGAPGPLVSSL
                     GLALFVAGWVIAYLALRENAFAATAVKHLARQTVVDTGVYGVVRHPMYAGGIPMLIGM
                     PLWLGSYASALLALVPIATVVVRALLEERFLRRELPGYVAYAERVRYRLVPFLW"
BASE COUNT          348 a          610 c          512 g          299 t
ORIGIN      
        1 cggcacgatc aaagcctgaa gcgaaaaatc gggagacgct caccggctga cttctcaccg
       61 tcttcgaccc ccgtcgccgg tgcctaccgc ccgccagccg ttctccggag ttacctccga
      121 agtacacttg actagctttc ggcgtccacg tacgagagcg tttgagtcgg ctacctttgc
      181 cagtcggttt gcgtctccac gattattcgt agcacacgcg ccgcgcgcgg acaatccgga
      241 aactgagttc gaggaatatg tggattgcgt ggataagtcc cgccaatcct cgatgttgcg
      301 caaccaacgt tgaagattac gaggggcggc aacaggatta ttctgccctc gtgatcactc
      361 gatctcgacg tcctcccaga acccgatcca atgatcgacc tgcttcatcg acgcgcgtgg
      421 cgcttcgtac gaccacgcgg cccgtaggct gcgtgcgtcg tccacgacga tgtcgtagaa
      481 ctggaccccg tgcgggcagg ccagatcgcc ctgcgtcttc ggcgccggcc ggagccaatc
      541 catgcggacg gtttcccgag gaaagtaccg gtaaccgcct acctcgaggg tgcgatcgct
      601 ctccgcgatc acctgaccct gccatatcgc cttaatggta accatcctcc tcgtggttct
      661 ctgtctattt ttattttagc aaccagttcc ttatgacgat ttagctggcc cgaccgccta
      721 ccagagaaac gggacgagcc ggtaccggac tcgctcggcg tacgcgacgt accccgggag
      781 ctcacgtctc agaaaccgct cctccaagag cgcccgcaca acgaccgtcg cgatcgggac
      841 cagcgccagc agcgcgctgg cgtacgaccc gagccacagg ggcatcccga tgagcatggg
      901 gattcccccc gcgtacatcg ggtgccgcac gacaccgtag accccggtat cgaccacggt
      961 ctgccgcgcc agatgcttca ccgccgttgc cgcgaaggcg ttctccctga gggccaggta
     1021 ggcgatcacc cagccggcga caaacagggc cagccccagg gacgacacga gcggcccggg
     1081 tgcccccaac aggtcgaaac gaaacacgtc caggggaatg aaggccacca acccgtaaaa
     1141 cgcggcgtaa aatagggtca cgacgatctt gtccgtgagc ggctgttccc tctggatagg
     1201 cagtttgagg cgttcctcga ccaggtcccg atgaaccggg agcaggctcg cgaccgcgta
     1261 aaccataccc gcgccgagga cgccgaggaa cacccagccg cgccaccagt tccccgtgcc
     1321 cgccgggacg aacagcacgg ccgcgaagaa cgacatccag aacaggaggt tggcgatcgc
     1381 tttgaaaagg agcggcatcg tcctccaccg ggccacgtcc gcggtccgat cgcgcacagc
     1441 atagtcctgc gggccgtccg ccggagatcg agcgcaggcc gtcaccgcgt gcgttgcggg
     1501 cggtgcggtc tcgacggtgg ccgcccggcc cggcacgaga gatccacggg gcccaggagc
     1561 gagggacccc gagatcccac gtgaccaatc gtgtcgctga accgtcctac ttgcgtctcg
     1621 cgaccctcac ctgttcgccc cgcccgagcg ctaacctgcc ttgtgtacga aacctacccg
     1681 ctctcccatc catgcgtgaa ttctccgagt ttcttgcccc cggcagagcc tcaggcaggg
     1741 tctccaaagg cgtggggcta taaagccca
//
LOCUS       DASQGH010000417         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41429, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000417 DASQGH010000000
VERSION     DASQGH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41429"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VEW91_08185"
     CDS             complement(<1..291)
                     /locus_tag="VEW91_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HYM91594.1"
                     /translation="MRAQVFPAVMLTIALTILTGLLYPLAITGVAQALFPHQANGSLI
                     VENGRVVGSHLIGQPFDDPKYFWGRLSATSPFPYNAASSSGSNLGPTNQALID"
     gene            complement(303..2351)
                     /gene="kdpB"
                     /locus_tag="VEW91_08190"
     CDS             complement(303..2351)
                     /gene="kdpB"
                     /locus_tag="VEW91_08190"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HYM91595.1"
                     /translation="MMWRIAIRDSFLRLDPRQQIRNPVMFVVEVTSVLTTILFFQALF
                     GRGEAPAPFIGSVSAWLWFTVVFANFAEATAEGRGKAQAEALRRARRETMAKRLHRPE
                     RGAPCETVSSTKLRKGDVVLVEVDEMMPADGEVIKGIASVDESAVTGESAPVIRESGG
                     DRSAVTGSTRVLSDWLIVRVTANPGEGFIDRMIGLIEGARRQKTPNEIALSILLAGFT
                     ILFLIVCATLLPYSVFSVGVAGRGTPVTITVLVALLVCLIPTTIGGLLSAIGIAGMDR
                     MIKRNVIALSGRAVEAAGDVDVLLLDKTGTITLGNRQAVEFVPVVGTDSRQLAEIAQL
                     ASLADETPEGRSIVVLAKDTYGLRGRTVGTGPEAPQGAKFMPFTAQTRMSGVDLDGLV
                     IRKGAPDAIAEHVRALGGSIPAGLNTVVDRIARSGGTPLVVARNNTILGVIHLKDIVK
                     GGIKERFLQLRRMGIRTIMITGDNPLTAAAIAAEAGVDDFLAQAKPEEKLRLIREHQA
                     GGRLVAMAGDGTNDAPALAQADVAVAMNTGTQPAREAANMIDLDSNPTKLIEVVELGK
                     QLLMTRGALTTFSIANDVAKYFAIIPAAFSSTYPVLSALNFMRLATPQSAISSAVIFN
                     ALIIVALIPLALRGVQYRPAGAARILRDNLIVYGVGGVVAPFIGIKLIDMILAALGLR
                     "
     gene            complement(2416..>4146)
                     /gene="kdpA"
                     /locus_tag="VEW91_08195"
     CDS             complement(2416..>4146)
                     /gene="kdpA"
                     /locus_tag="VEW91_08195"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HYM91596.1"
                     /translation="RTFLDPVVRPLERLLYRLCRIDPAEDMPWQTYTIAMLVFTVAGA
                     FLLYALQRLQGVLPIHDFHLNPQGFGAVSPDLALNTAVSFTTNTNWQNYGGETTMSYL
                     TQMAGLTMKNFVSAAVGLAIMVALIRGLARRSAQAIGNFWVDTVRSCLYILLPLSVVL
                     ALALASQGVVATLSPYRTATLLQPTSYEEPITDANGKRVLDAKGQPKTKTVKVTEQVI
                     AVGPAASQIAIKQLGTNGGGFFNANSSHPLENPTPFSNFLEMLAILVLPAAVCYTFGR
                     MIGDTRQGWALLAVMTLIFLPLLAVTEGFEQAGNPLFRAAGVDQTASDFQPGGNMEGK
                     EVRFGIADSALWATATTATSNGSVNSMHDSYTPIAGMVPLVMMHLGEVIYGGIGSGLF
                     DMLVFAILAAFVAGLMVGRTPEYLGKKIEAFEMKMASVVVLVMPTIVLIGTALGVATV
                     AGKAGASNPGPHGFSEILYAFTSQGNNNGSAFAGISSNTPFYNVVGAVVMWLGRFWEI
                     VPVLAIAGSMAAKKKVPASPGTLPTHTGQFVVWLAAVVIIVGALSFLPALALGPIVEH
                     LLLQAGTVFK"
BASE COUNT          709 a         1405 c         1356 g          676 t
ORIGIN      
        1 atcgatcagc gcctgattgg tcggccccag gtttgagccg ctcgacgagg ccgcattgta
       61 cggaaacggg gacgtagcgg acaggcgacc ccagaagtac ttggggtcgt cgaagggttg
      121 accaatcaga tgtgagccca cgacgcgacc gttttccacg atcaggcttc cgttcgcttg
      181 gtgcgggaac agggcctggg ctaccccggt gatggccaag gggtagagca gcccggtgag
      241 aatcgtcagc gcgatcgtga gcatcacggc cggaaagacc tgcgcacgca tgatcgccct
      301 ccctacctga ggcccagcgc cgccaggatc atatcgatca gcttgatccc gatgaagggc
      361 gcgaccaccc cgccgacgcc gtacacgatc aggttgtccc ggaggattcg cgcggctccc
      421 gcgggccggt actgcacgcc gcgcaacgcc agcgggatca gggcgacgat gatcaacgca
      481 ttgaagatca ccgccgacga aatagcgctc tgcggcgtcg ccaggcgcat gaagtttaga
      541 gccgagagca ccgggtaggt cgaggagaac gcggcaggga tgatggcaaa gtacttggcg
      601 acgtcgttgg cgatgctgaa ggtggtcagg gctccgcgcg tcatcagcag ctgcttgccg
      661 agctcgacga cctcgatgag cttcgtaggg ttgctgtcga ggtcgatcat gttcgccgcc
      721 tcgcgggcgg gctgggtccc ggtgttcatg gccaccgcca cgtcggcctg cgcgagcgcc
      781 ggggcatcgt tggtcccatc cccggccatc gccacgagcc ggcctccggc ctggtgctcg
      841 cggatcagtc ggagcttctc ctcgggcttg gcctgggcca ggaagtcgtc caccccggcc
      901 tccgcggcga tcgccgccgc ggtcagcggg ttgtcccccg tgatcatgat cgtccggatg
      961 cccatccgtc ggagctgcag gaaccgctcc ttgatgccgc ccttgacgat gtccttgagg
     1021 tggatgacac cgaggatggt gttgttgcgg gcgacgacga gcggggtccc tccggaccgc
     1081 gcgatccggt cgaccaccgt gttgagcccg gccgggatgc tcccccccag cgcccgcacg
     1141 tgctcggcga tggcgtccgg cgcccccttc cggatgacga gaccatcgag atcgacgccg
     1201 ctcatccggg tctgtgccgt aaacggcatg aacttggccc cctgcggcgc ttcgggaccc
     1261 gtcccgaccg tccgaccccg gaggccgtac gtgtccttcg ccagcacgac gatgctgcgc
     1321 ccttcggggg tctcatcggc caacgaggcc agctgcgcga tctcggccag ttgccgcgag
     1381 tccgtgccca ccaccgggac gaactccacg gcttgacgat tgccgagcgt aatcgtgccg
     1441 gtcttgtcca acagcagcac gtctacatca cccgcggcct cgaccgcccg tcccgagagc
     1501 gcgatcacgt tgcgcttgat catgcggtcc atccccgcga tcccgatcgc gctgagaagc
     1561 ccaccgatgg tggtggggat caggcacacc agaagggcga cgaggaccgt gatcgtcacc
     1621 ggggtccccc gaccggccac gccgacgctg aacaccgagt acggcagcag cgtcgcgcag
     1681 acgatcaaga agagaatcgt aaaccctgcc agcaggatgc tgagggcgat ctcattcggg
     1741 gtcttttggc gccgggcccc ttcgatcagg ccgatcatgc gatcgatgaa gccctcgccg
     1801 gggttggcgg tcacccgcac gatcagccag tccgacagca cgcgggtgct gccggtcacc
     1861 gcgctccggt cccccccgga ctcgcggatc accggggcgc tttcccccgt caccgcgctc
     1921 tcgtcgacgg aggcgatccc cttgatcacc tccccgtccg ccggcatcat ctcgtcgacc
     1981 tccacgagca ccacatcgcc cttccggagc ttcgtggacg acacggtctc gcacggggcc
     2041 ccccgctcgg ggcggtgcag gcgtttcgcc atcgtctccc gacgggcccg ccggagcgcc
     2101 tcggcctggg cctttccccg tccttcggcc gtagcctcgg cgaagttggc gaagacgacg
     2161 gtgaaccaca gccaggcgga aacgctgccg atgaagggcg cgggagcctc cccccggcca
     2221 aacagcgcct ggaagaagag gatcgtggtg agcacgctcg tgacctcgac gacgaacatc
     2281 accgggtttc ggatctgctg ccgcgggtcg agccgcagga acgaatcacg gatcgcgatg
     2341 cgccacatca tcgcggtctg cgcggccttg gccttgggct ccacctgcac actctgtgcg
     2401 ctcatggccg tctcctcact tgaagaccgt gcccgcctgg agcaacaggt gctcgacgat
     2461 cgggccgagc gccagcgccg gcaggaagct gagggccccg acgatgatga ccaccgcggc
     2521 gagccagacg acgaactgcc cggtgtgggt gggtagcgtg ccgggactgg ccggcacctt
     2581 tttcttcgcg gccatggagc cggcgatcgc gagcactggc acgatctccc agaaccgccc
     2641 cagccacatg accacggctc ccacaacgtt gtagaacggg gtgttgctgc tgatcccggc
     2701 gaacgcgctc ccgttgttgt tgccctgcga ggtgaatgcg tacaggatct cactgaatcc
     2761 gtgcggcccc gggttggagg caccggcctt cccggccacg gtcgccaccc ccagggccgt
     2821 cccgatgagc acgatcgtcg gcatgaccag gaccacgacc gaggccatct tcatctcgaa
     2881 ggcctcgatc ttcttgccga gatactccgg tgtgcgcccc accatcaatc ccgcgacgaa
     2941 ggcggcgagg atcgcgaaga cgagcatgtc gaaaaggcca ctcccgatcc cgccgtagat
     3001 gacctcgccg agatgcatca tcacaagcgg caccatcccg gcgatgggcg tgtacgagtc
     3061 atgcatcgag ttgaccgagc cattcgaggt ggccgtcgtc gccgtcgccc acagcgcgga
     3121 gtcggcgatg ccaaaccgga cctccttgcc ctccatgttc ccgcccggct ggaagtcgct
     3181 cgcggtctgg tccacgcccg cggctctgaa gagcgggttc cccgcctgct cgaatccctc
     3241 ggtcaccgcc agcagcggaa ggaagatcag cgtcatcacc gcgaggagcg cccacccctg
     3301 ccgggtgtcg ccgatcatcc tcccgaacgt gtagcagacc gccgcgggca acaccaggat
     3361 cgcgagcatc tcgagaaagt tcgagaacgg ggttggattt tcgagcgggt gggacgagtt
     3421 cgcgttgaag aacccgccgc cgttcgtgcc gagttgcttg atggcgatct gtgaggcggc
     3481 cgggccgacg gcgatcacct gctcggtcac cttgacggtc ttcgtctttg gctggccctt
     3541 ggcgtcgaga acacgctttc cgttcgcatc cgtgattggc tcttcatagg aagtcggctg
     3601 caacagcgtc gcggtcctgt acggcgacag ggtcgccacc acgccttgag aagccagggc
     3661 cagcgccagg acgacggaaa ggggaagcag gatgtacaga cagctccgca ccgtatcgac
     3721 ccagaagttg ccgatggcct gcgccgagcg gcgtgccagg ccgcggatca gggccaccat
     3781 gatggccaac ccgaccgccg ccgagacgaa gttcttcatg gtgagccccg ccatctgcgt
     3841 caggtagctc atcgtggtct cgccgccgta gttctgccag ttggtgttgg tcgtgaagga
     3901 cacggccgtg ttcagggcca ggtcgggcga gacggcgccg aacccctgcg ggttgaggtg
     3961 aaaatcgtgg atcggcagga ccccctgcag ccgctgcagc gcatacagca ggaacgcgcc
     4021 cgcgacggtg aacacgagca tggcgatcgt gtaggtctgc cacggcatgt cttctgcggg
     4081 gtcgatccgg cagagccggt agagcagccg ctcgaggggg cgcaccacgg gatcgaggaa
     4141 ggtgcg
//
LOCUS       DASQGH010000418         5885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21520, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000418 DASQGH010000000
VERSION     DASQGH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21520"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VEW91_08200"
     CDS             complement(<1..506)
                     /locus_tag="VEW91_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYM91597.1"
                     /translation="MQAQFGRQASWYTVSSAHRDAAGLTEVVRLASPFPQALALDLAT
                     GTGFAALAIAPRCRRVIGLDLTLGMVLQARRLAGERGVANVRFCLGDVEAVPFSDSTF
                     DLVICRLAAHHFPDLPGAISEMARVVKRGGRVVLEDTCAPEAPELAALMNDWEVRRDP
                     SHVADHPP"
     gene            590..1003
                     /locus_tag="VEW91_08205"
     CDS             590..1003
                     /locus_tag="VEW91_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91598.1"
                     /translation="MSLSQWHAQSLLLILTLTGDVVCIALAVFGDLTRRHTMPHLFWW
                     VLWLAQVPLGVQAILGIGLLAGGVRPRTPYHLMYGGLILLTLLALYGLRPGGWIRRAF
                     VRDERAYRESRWLMLLCAFLAALVGRAYMTGVLGR"
     gene            complement(1047..2345)
                     /gene="dcuC"
                     /locus_tag="VEW91_08210"
     CDS             complement(1047..2345)
                     /gene="dcuC"
                     /locus_tag="VEW91_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HYM91599.1"
                     /translation="MFLAIAAGVITVAVFVAIVRRVEVRLALLTGGLAMAVLSLNPQA
                     WSNAFLSGMTISGLITVILPVMGFAAVMELTGCDRHLVHLVTRPLSRVGPFLVPGAML
                     ATFVINTALPSAAGCAAAVGVVLIPTMMAAGVHPALAAAAVLAGTWGSIFSPGSAHPA
                     VIADISKVSVMDVILAHRAATIPAMLLIAALLFAESLVLHEGAGASRRPVRTIAGGSV
                     DTTVSAPRIDVVRALIPMVPLALLLLTVPQFHLTTRWLPGGFSVLEAMTIGVFLGMLV
                     TMMSPTKVTQSFFDGMGRAYGNVMGIIIAATVFVGGLDAAGGIKALIAAMTSVKWAVP
                     VAGTWGPMLLAVLSGSGDAATLAFNKAVTIHAAQFGISPVALGNLAWIGGALGRSMSP
                     VAAAAIIAAGFAKVSPFELAKRNAVPMVAAAVLTMMVLGR"
     gene            complement(2547..3737)
                     /locus_tag="VEW91_08215"
     CDS             complement(2547..3737)
                     /locus_tag="VEW91_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYM91600.1"
                     /translation="MAGVTVEELMREPEVAKALAWIDSRRDAIIEEAIRICEIPAPPF
                     EEAARAAYVRQRFEALGLADVRIDETGTVEGRRPGAGGRLGVALSAHLDTVFPRGTDV
                     TVKRRGTQLHAPGIGDNSVSVAALLAVLEAMNAAGLRTAGDLYLAGNTGEEGLGNLRG
                     MKAFIAAVRDRVGAAIVIDGMRINRIVHVAVGSRRYEVTFTTPGGHSWADFPAPSAVH
                     LMGRAIAEISRLDVPVSPKTTYNVGVVTGGTTVNSIAGEAKMLVDMRSVDPGPLADVE
                     RRLLEIVKRTARDGHGEAGWTLVGDRPAGAIPETHPVVQTCAAVHRALGLDVYTEPAS
                     TDHNAPLALGIPSVCLAVTAGEHEHRLDEYIETAPLAVGIRKILVATVAVSKEIARPA
                     ETPA"
     gene            4218..>5885
                     /gene="ilvB"
                     /locus_tag="VEW91_08220"
     CDS             4218..>5885
                     /gene="ilvB"
                     /locus_tag="VEW91_08220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HYM91601.1"
                     /translation="MTRPTMPGTRPAATRITKRMSGARALIECLERVGVDVMFGHPGG
                     AALPLYDALYDARQIRHLLVRHEQVAAHAAVGYHRVTGKVGVCMATSGPGATNLLTGI
                     TDAMADSAGIVAITGQVSTLVIGTDAFQEADVMGMTMPITKHSSLVQTAVDLPRLVLQ
                     SFLIAGTGRPGPVLVDIPRDVAQTEAEFEFPEQVTLRSGKVPPAVPSPNQIAAAAQLL
                     SQASRPIIYAGGGIISSNAAAELAALARQARIPVTTTLMGKGGFPETDPLSLGMLGMH
                     GTAYANYAINAADVILAVGVRFDDRVTGRLKDFAPHARFIHIDIDPAEIGKNKPAHVP
                     IVGDAREALRVLAPQLAPLDCEPWHRQIAEWKERYPLRYRQEGETVKPQFAIDEIYRL
                     TKGQAIVATDVGQHQMWAAQFYLTTQPRTWVTSGGLGAMGFGFPASIGAQMGRPDALV
                     CAFVGDGGFQMTMQDLASAVEWGLPIKIYIINNKALGMVRQWQQLFYRERYSHVWLKN
                     PDFAKLAEAFGAVGITVRRPDEVAPAIERSLQVTDRPCLVDFACDPEE"
BASE COUNT          916 a         2078 c         2024 g          867 t
ORIGIN      
        1 ctcgggggat ggtcggcgac atgggaggga tcgcgccgca cctcccagtc gttcatcagg
       61 gcggccagct ccggggcttc gggggcgcag gtatcctcga ggacgacgcg ccccccacgc
      121 ttcacgacgc gcgccatctc cgaaatcgcc ccgggcaggt cggggaaatg gtgggcggca
      181 aggcggcaga tcacgagatc aaacgtgctg tcggaaaacg gcacggcttc cacatcaccg
      241 aggcagaacc ggacgttggc gaccccacgc tccccggcga gtcggcgcgc ctggaggacc
      301 atgccaaggg tgagatccag tccgatgacc cggcggcacc ggggcgcaat ggcgagggcg
      361 gcgaacccgg tgcccgtggc gaggtcgagg gccagcgcct gcgggaaggg ggaggcgagg
      421 cgcaccacct ccgtcagccc cgcggcgtcg cggtgggccg agctgaccgt gtaccacgac
      481 gcctgccgcc caaactgggc ctgcacggcc cgctttgaat cctcgctcac cgaagcggca
      541 gggcaggagc gggggacgat tgggcgaacc tcatcagggt gatgctgtcg tgagcctttc
      601 gcagtggcat gcccaatcgc tcctcttgat cctcacgctc acgggggatg tggtctgcat
      661 cgccctggcg gtgtttgggg acctgacgcg acgccacacc atgccccatc tcttctggtg
      721 ggttctgtgg ctggcgcagg ttcccctggg cgtgcaggcg atcctcggca taggcctgct
      781 cgcgggtggt gtccggccgc gaacacccta ccacctgatg tacggggggc tgatcctgct
      841 cacgctgctc gcgctgtacg gcctcaggcc cggagggtgg atccgccggg cgttcgttcg
      901 ggacgagcgc gcgtaccgcg agtcgcggtg gctcatgctc ctgtgcgcgt tcctggccgc
      961 cctggtcggc cgcgcataca tgacgggggt cctgggccgg taaccttccc ccgggaggtc
     1021 tcggtggttg ctgtcgcgtg acccgcctag cgccccaata ccatcatcgt gaggaccgcc
     1081 gccgccacca tcggcaccgc attccgcttg gccagttcga acggactgac cttggcgaac
     1141 cccgcggcga tgatcgccgc cgccgcgacc ggagacatgc tccggcccag cgcgccgccg
     1201 atccacgcga ggttgcccag agcgaccggc gagatcccga actgcgcggc atggatggtc
     1261 accgccttgt tgaaggcaag cgtggccgcg tccccggagc cgctcagcac cgccagcagc
     1321 atcggccccc aggtcccggc gaccggcacg gcccacttga ccgaggtcat cgccgcgatc
     1381 agcgccttga tcccccccgc ggcgtcgagc ccgcccacga agaccgtggc agcgatgatg
     1441 atgcccatca cgttcccgta cgcccggccc atcccgtcga agaagctctg cgtgaccttg
     1501 gtgggcgaca tcatggtgac gagcatcccg aggaacacgc cgatcgtcat cgcctccagg
     1561 accgagaatc cccccggcag ccagcgtgtc gtcaggtgga actgcggcac cgtcagcagc
     1621 agcaacgcga gcggcaccat cgggatgagc gcccggacga cgtcgatgcg cggagcggac
     1681 acggtcgtat ccaccgaccc gccggcgatc gtgcgcaccg gccggcggga cgcgccggcg
     1741 ccctcgtgca gcacgaggct ctccgcgaac aggagcgcag cgatcagcaa catggcgggg
     1801 atcgtcgcgg cccggtgcgc gaggatgacg tccatgacgg acactttcga gatgtccgcg
     1861 atgacggccg ggtgtgcgga gcccgggctg aagattgagc cccaggtgcc cgcgagcacg
     1921 gccgcggcgg cgagcgccgg atgaacgccg gccgccatca tcgtcggaat cagcacgacc
     1981 ccgacggctg cggcgcaccc cgcggcgctg gggagcgctg tgttgatcac gaacgtcgcg
     2041 agcatcgccc cgggcacgag gaagggcccg acccgcgaca gcggccgggt gaccagatga
     2101 acgaggtgcc gatcgcaccc cgtcagctcc attacggccg cgaaccccat caccggcagg
     2161 atcacggtga tgaggccgga gatggtcatc ccgctgagaa acgcgttgga ccacgcctgc
     2221 gggttgagcg agaggactgc catcgcgagc ccgccggtca gcagtgcgag gcgcacctcc
     2281 accctgcgca cgatggccac aaacacggcg acggtgatca ctccggctgc gatcgctaga
     2341 aacatcttcc tccccccttt tgcggggccg tcacggccgt gaagtgggcc tggcgaggag
     2401 gagcggacga tccggggccg catcctcgtc ggccctcagg cgtgatggtc tgcgcgactt
     2461 cactgcggag cgtatcacac tcgccgtcct agggcccatc gggcgttcgc ccgattctgc
     2521 cccaggccgg tgccgcccgg cgcgcgctag gcgggcgttt cggcgggccg ggcgatctcc
     2581 ttcgacaccg cgaccgtcgc caccaggatc ttccggatcc ccacggccag cggtgccgtc
     2641 tcgatgtact catccagtcg gtgctcgtgc tcacccgcgg tcaccgccag gcagacgctt
     2701 gggatgccga gtgcgagagg cgcgttgtgg tcggtgctgg ccggctccgt gtacacgtcg
     2761 agtccgaggg cgcggtggac cgccgcgcac gtctgcacga ccgggtgggt ctccgggatc
     2821 gccccggccg ggcggtctcc gacgagggtc cagcccgcct cgccgtgccc gtcccgtgcc
     2881 gtgcgcttga cgatctccag cagccggcgc tccacgtccg cgagggggcc ggggtcgacg
     2941 gaccgcatgt ccaccagcat cttcgcttca cccgcgattg agttgacggt ggtgccacct
     3001 gtgacgaccc cgacattgta cgtggtcttc ggtgacaccg gcacgtccag ccgcgagatc
     3061 tccgcgatcg cccggcccat caggtgcacc gcgctcggtg cgggaaaatc ggcccagctg
     3121 tgcccacccg gggtcgtgaa cgtcacctcg tatcggcggc tcccaactgc gacgtgcacg
     3181 atccggttga tccgcatgcc gtcgatcacg atcgccgcgc ccacccggtc ccggacggcc
     3241 gcgatgaacg ccttcatccc ccggaggtta cccagccctt cctctcccgt gtttcccgcg
     3301 aggtagaggt cgcccgccgt gcgcagccca gctgcattca tcgcctcgag cacggcgagc
     3361 agcgcggcga cggacacgct gttgtcgccg atgcctgggg cgtgcagctg ggtcccccgc
     3421 cgcttgaccg tgacgtccgt cccgcggggg aacacggtat cgaggtgcgc ggacagcgcg
     3481 accccgaggc ggcccccggc ccccggccgg cggccctcca ccgtgccggt ctcgtcgatt
     3541 cggacgtccg ccagccccag cgcctcgaag cgctgccgca cgtacgctgc ccgcgcggct
     3601 tcctcaaacg gcggcgctgg gatctcgcag atccggatcg cctcttcgat gatcgcgtcg
     3661 cgccggctgt cgatccaggc gagcgccttc gcgacttcgg gctcgcgcat caactcctcg
     3721 accgttaccc cagccatcgt cgcctccgcc ggctcgtcat cgtcccgtgc gagcgtgcac
     3781 ggtccagcgc cgctccgcgc ggtgttcttc gactcgtcgg caagcacacc ctggtccgca
     3841 gcccgacgat agccccaagt ttcttgacaa acccgggggg gtttggtaca aatggcgtta
     3901 actccatagc aggctgtgac gagagcagga cgcggcacac cggatccgag agagctgggt
     3961 caggtgcgag ccagtatccg gggccgcgac cactcatctc tgagccgcgg gaaccaaggg
     4021 ccgccgtaag gcgcgccgag tagcacccgc cggaaacccg ccgagagagg ggaagagggc
     4081 cgcgccgggc cacgattgcc ggggcggaac tagggtggta ccgcggggag acccgtcctt
     4141 agagggctcc gcgcggtttg tatttttttg ggacaccgtg cacgccgggg ctatggcgga
     4201 tgaaggggga gaggatcatg actcggccaa cgatgcccgg gacgaggccc gcggcgaccc
     4261 gcatcaccaa gcggatgtcc ggggcccgcg cgctcatcga gtgcctcgag cgggtgggag
     4321 tggacgtgat gttcgggcat cccggcgggg ccgcgctgcc gctttatgat gcgctgtacg
     4381 acgcccggca gatccggcac ctcctggttc gccacgagca ggtggccgcg cacgctgcgg
     4441 tcgggtatca ccgggtcacc ggaaaggtcg gcgtctgcat ggcgacctcg ggcccgggcg
     4501 cgacgaacct cctgaccggg atcaccgacg ccatggcgga ctccgccggc atcgtcgcga
     4561 tcaccggaca ggtgagcacc ctcgtgatcg gcaccgatgc gttccaggaa gccgacgtga
     4621 tgggcatgac gatgcccatc accaagcaca gctcgctcgt ccagacggcg gtggaccttc
     4681 cccgcctggt gctgcaatcg ttcctgatcg ccggcaccgg gcgccccggc ccggtcctgg
     4741 tggacatccc ccgcgacgtc gcccagaccg aggcggagtt cgagttcccc gaacaggtca
     4801 cgctgcggag cgggaaggtg ccgcctgcgg tgccgagccc caaccagatc gccgccgcgg
     4861 cgcagctcct gagccaggcc tcgcggccga tcatctacgc ggggggcggg atcatcagca
     4921 gcaacgccgc cgcggaactg gctgcgctcg cccgccaggc ccgcatcccg gtcaccacga
     4981 ccctgatggg gaaggggggg ttcccggaga ccgacccgct gtcgctgggc atgctcggca
     5041 tgcacggcac cgcctacgcc aactacgcga tcaacgcagc ggacgtcatc ctcgcggtcg
     5101 gcgtgcggtt cgacgaccgg gttaccggcc ggctcaagga tttcgcgccc cacgcccgat
     5161 tcatccacat cgacatcgat ccggcggaga tcggcaagaa caagccggcc cacgtgccga
     5221 tcgtcgggga tgcccgggag gcactgcggg tgctcgcccc ccagctcgcg ccgctggact
     5281 gcgagccatg gcaccggcag atcgccgagt ggaaggagcg ctatcccctg cggtaccggc
     5341 aggagggcga gacggtcaag ccccagttcg cgatcgacga gatctaccgg ctgaccaagg
     5401 gccaggcgat cgtcgccacc gacgtcgggc agcaccagat gtgggccgcc cagttctacc
     5461 tgaccacgca gccgcgcacg tgggtcacct ccggcgggtt gggcgcgatg ggattcgggt
     5521 tccccgcctc gatcggggcg cagatgggac gcccggacgc cctggtctgc gcgtttgtgg
     5581 gggacggcgg gttccagatg acgatgcagg atctcgccag cgcggtggag tggggcctgc
     5641 cgatcaagat ctacatcatc aacaacaagg cgctcggcat ggtccggcag tggcagcaat
     5701 tgttctaccg ggagcggtac tcgcacgtgt ggctgaagaa tcccgacttc gcgaagctgg
     5761 cggaggcgtt tggcgcggtg ggcatcaccg tccgccggcc tgacgaggtg gccccggcca
     5821 tcgaacggtc gctccaggtc accgaccggc cgtgcctcgt ggatttcgcc tgcgacccgg
     5881 aggag
//
LOCUS       DASQGH010000419         1535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35792, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000419 DASQGH010000000
VERSION     DASQGH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35792"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VEW91_08225"
     CDS             <1..253
                     /locus_tag="VEW91_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYM91602.1"
                     /translation="RCPVPTRNPRINVILERPLYRSVERLARRDGVSLSTKMRDLVRE
                     ALELVEDAGLAALAERREGSFTRKSVLSHDQVWGRQRTR"
     gene            428..535
                     /locus_tag="VEW91_08230"
     CDS             428..535
                     /locus_tag="VEW91_08230"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91603.1"
                     /translation="MGGYRVVYAIRGNEVLILEIRHRSTVYIDVRSRLP"
     gene            complement(565..>1535)
                     /locus_tag="VEW91_08235"
     CDS             complement(565..>1535)
                     /locus_tag="VEW91_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYM91604.1"
                     /translation="GLYAAYYAGFRTLRTKIVESLNALGGQVTALYPEKWIYDVAGFP
                     KILGRNLVDNLAEQAMQYHPTVCLGETAGSLAIQPDGTLQIASDGTAHLTRTLLITAG
                     IGAFHPKTFKNPLIDGFEGRGLYYFVRSFQEFKDRRVLIVGGGDSAVDWALGLVGIAR
                     SITLIHRRDQFRAHEDSVQKLQTSPIQLKTPYELRRLEGGSQVARAVIYQNKTNVDET
                     IEVDAVIASLGFLSTLGPIQGWGLELEHDSIRVNTRMETNLRGVYAAGDIVTYPGKVK
                     LIATGFGEAATAINNAAAYINPKASVFPGHSSSQDVHAKPKTPVGT"
BASE COUNT          236 a          498 c          513 g          288 t
ORIGIN      
        1 gaggtgcccc gtgccgacgc ggaatccacg gatcaatgtc atcctggagc ggccactgta
       61 tcgttccgtc gagcggctgg cacggcggga tggggtctcc ctctcgacaa aaatgcgcga
      121 tcttgtgcgc gaggcgctcg agctcgttga ggatgccggg ctcgccgccc tcgccgagcg
      181 gcgcgagggg tcgttcacgc ggaagtcggt cttaagccac gatcaggtct gggggcgcca
      241 gcgcacccgg taatccgggt gttctgcctt ctttaccacc cggccgtcgc ctcagacgat
      301 ctcccggtca tacctcgagg cgtccaggct ggcatcgccc gagcgatcga gcggcgcctt
      361 accacagatc cccatcgagt gggcgccccg ctccgggggc acccttaaag gctactggaa
      421 gcttcggatc ggcggctacc gagttgtgta cgcgatccgg ggtaacgaag tcttgattct
      481 cgagatccgc caccgcagca cggtgtacat cgacgtgcgg tcgcgtctcc cttaggcacg
      541 tcgctcgtca agagggctgg aggctcacgt cccgactggc gtcttcggtt tggcgtgcac
      601 atcctgcgaa ctgctgtggc cggggaagac gctcgccttg gggttgatgt aggcggcggc
      661 gttgttgatc gccgtggcgg cctcgccgaa cccggtggcg atgagcttga ccttgcccgg
      721 gtaggtgacg atatccccgg ccgcgtacac gccccgcagg ttggtctcca tgcgcgtgtt
      781 gacccggatg ctgtcgtgct ccagttcgag cccccatccc tggatcggcc cgagggtgct
      841 caggaacccg agggacgcga tgaccgcatc gacctcgatc gtttcgtcga cgttggtctt
      901 gttctggtag atgacggccc gcgcgacctg gctccccccc tccaggcgcc gcagctcgta
      961 gggggtcttc agctggatgg gagaggtctg gagcttctgc accgagtcct cgtgggcccg
     1021 gaactggtcc cggcggtgga tcagggtgat cgaccgcgcg atccccacga gcccgagcgc
     1081 ccagtcgacg gccgagtctc ccccgccgac gatcaggacc cgccggtcct tgaactcctg
     1141 gaagcttcgc acaaaatagt acaggccccg gccctcgaac ccatcgatca gagggttctt
     1201 gaacgtcttg gggtggaagg cgccgatccc cgcggtgatc agcaacgtgc gggtgagatg
     1261 cgccgtcccg tctgaggcga tctggagggt gccgtcgggc tggatggcga gcgagcccgc
     1321 cgtctctccc aggcagaccg tgggatggta ctgcatcgcc tgctcggcga ggttgtccac
     1381 cagattccgg ccgaggatct tgggaaaccc cgcgacgtcg tagatccact tttccggata
     1441 cagggcggtg acctgtcccc cgagcgcgtt gaggctttcc acgatcttcg tccggagcgt
     1501 gcgaaacccg gcgtagtagg cggcgtacag accca
//
LOCUS       DASQGH010000420         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13309, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000420 DASQGH010000000
VERSION     DASQGH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13309"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEW91_08240"
     CDS             complement(<1..302)
                     /locus_tag="VEW91_08240"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYM91605.1"
                     /translation="MRALVQRVRRAEVRGAAPQGELIGQIGAGMVILLGVGTDDSTED
                     ARLLAGRLAHLRIFDDDAGRLNRSVLDVGGAVLSVPQFTLYADTSGGRRPSFVRAAR"
     gene            complement(313..1500)
                     /locus_tag="VEW91_08245"
     CDS             complement(313..1500)
                     /locus_tag="VEW91_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYM91606.1"
                     /translation="MSRSVSPDAALPGVSALARAWETYRDEFPIFQETTYLNTCSLCA
                     LGTRVRRAVDRCLDLWNTTGGSAWYGPWWQELDRLRESFARLIGSDREEVALFPSITA
                     GLSAVASCFDYRARPRVVVADIDFPTVAYQWLAKRGRGVEVAFVRSPDRLTVPLALFE
                     RAIDDRTVCVATSHVYFQSGAIQDIAALARLAHARGAYLLVDAYQSAGQLPIDVRAAG
                     ADFLVTGGLKWLLGGPGIAYVYARRALHPQLQPEVFGWFGHRDQFAFDVTRLEYADDA
                     RRLEGGTPAIAAVYAGRAGLEYVHELSPARLRARQVELTTALVEALRSAGVAPRLAGG
                     VAAHAGIITVPVPDPPAAVAGLASQRIIVDQRPGVVRLSPYFYNTVEDIERAVAGLRR
                     YVR"
     gene            complement(1497..1922)
                     /locus_tag="VEW91_08250"
     CDS             complement(1497..1922)
                     /locus_tag="VEW91_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM91607.1"
                     /translation="MHDFLTVGAAAPLQEAGVTALRFPRQYYADLAAMYQRKRDTLLG
                     ILEEAGFRCLVPSGAYYIMCEITPFGYEDDYEFTMHLIRDIGVAPVPGSSFFHDPASG
                     RRFVRFAFPKREETFDEVRTRLGRLRARAGAAPKEGAAR"
     gene            complement(1907..2692)
                     /locus_tag="VEW91_08255"
     CDS             complement(1907..2692)
                     /locus_tag="VEW91_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM91608.1"
                     /translation="MITTASRVGVFTESVIREMTRLAAEHGGINLAQGFPDFPAPAEL
                     VEAAARALREGYNQYAITWGAPAFRQAIAEKVRRFNGITVDPDRHITVTCGATEAMMA
                     TLLAVVNPGEEVIIFEPFYENYGPDTKLSGAVPRFVRLRLEPDFPFDPDEIRAQISPR
                     TKAIIVNSPHNPTGKVFSQTELDQVADLCHRHNLLAITDEVYEHILYDGHTHRTLASL
                     PGMAERTVIVNSLSKSYSVTGWRVGWTIAPPTITTRSARCMIS"
BASE COUNT          450 a         1022 c          979 g          416 t
ORIGIN      
        1 cgcgccgcgc gaacgaagct cggccgccgc cccccggagg tatccgcgta cagggtgaac
       61 tggggcacgc tgaggactgc gccaccgacg tccaaaaccg agcggttgag gcgcccggca
      121 tcgtcgtcga agatgcgaag atgcgccagc cgtccggcga ggagccgggc atcctcggtc
      181 gagtcgtctg tcccgacgcc cagcaggatc accatgccgg ccccgatctg cccgatgagc
      241 tccccttggg gcgcggcgcc gcgcacctcg gcccgccgga cccgctggac cagcgcccgc
      301 atccgcgcgg gcctagcgga cgtaccgccg cagcccggcg acggcgcgct cgatatcctc
      361 gacggtattg tagaagtacg gcgagagccg gacgaccccg ggccgctgat cgacgatgat
      421 gcgctgcgac gccaagcccg cgaccgcagc cggcgggtcc ggaaccggca cggtgatgat
      481 gccagcatgc gccgccaccc ctcccgccag ccggggggca acccccgcgc tgcggagcgc
      541 ctcgaccagt gcggtcgtca actcgacctg ccgggcgcgc agccgcgccg ggctcagctc
      601 gtgaacatac tccagcccgg cccggcccgc gtacaccgcg gcgatcgccg gcgtcccacc
      661 ctcgaggcgg cgcgcgtcat ccgcgtattc caagcgagtc acgtcgaagg caaactggtc
      721 acggtgtccg aaccacccga agacctccgg ctgcaattgc gggtgcaggg cccggcgtgc
      781 gtacacgtag gcgatgcctg gccctccgag caaccacttg aggccgccgg tgacgaggaa
      841 gtcggcgccc gcagcccgga cgtcgatcgg caactgcccc gcggactggt acgcgtccac
      901 gagcaggtac gcgccgcggg cgtgcgcgag acgcgcgagc gcggcgatgt cttgaatcgc
      961 cccgctctga aagtagacgt gcgaggtcgc gacgcacacg gtgcggtcgt cgatggcgcg
     1021 ctcgaagagg gcgaggggga cggtcagccg gtcggggctg cgcacaaacg cgacctcgac
     1081 ccctcgcccg cgcttcgcca accattggta tgcgacggtg gggaaatcga tgtcagcaac
     1141 cacgacccgc gggcgcgcgc ggtagtcgaa gcaactcgcc accgccgaga ggccggcggt
     1201 gatcgagggg aagagcgcta cctcctcccg gtcgcttccg atgagcctgg cgaacgattc
     1261 ccggagccga tccagttcct gccaccacgg cccgtaccac gcggagcccc ccgtggtgtt
     1321 ccagaggtcc aggcaccggt ccaccgcgcg ccgcacccgc gtgccaagcg cgcagagcga
     1381 gcacgtgttg agataggtcg tctcctgaaa gatagggaac tcatcgcgat atgtctccca
     1441 cgcccgcgcc agcgccgaca cacccgggag ggcggcatcg ggagacaccg accggctcac
     1501 cgggccgcgc cctctttcgg cgcggcgccg gctcgggccc gcagccgtcc caggcgcgtc
     1561 cggacctcgt cgaacgtctc ttcccgcttg gggaacgcga accgcacgaa ccgccggccg
     1621 gaggcgggat cgtggaagaa ggacgacccc gggaccgggg cgaccccaat gtcccggatc
     1681 aggtgcatcg tgaactcata gtcgtcctcg tacccaaacg gggtgatctc gcacatgatg
     1741 tagtaggcgc cggacggcac caggcacctg aatcccgctt cctcgaggat cccgaggagc
     1801 gtgtcccgct tccgctggta catcgcggcc agatcggcgt agtactggcg cgggaatcgg
     1861 agggccgtga ccccggcttc ctgcagcggc gcggcggccc ccaccgtcag gaaatcatgc
     1921 accttgcgga tcgcgtggtg atcgtggggg gcgcgatcgt ccatccgacc cgccacccgg
     1981 tcacgctgta gctcttcgac aggctgttga cgatcacggt gcgctcggcc atcccgggca
     2041 gggaggcgag ggtccggtgg gtgtggccgt cgtagaggat gtgctcgtac acctcgtcgg
     2101 tgatcgccag caggttgtgc cgatgacaga ggtcggcgac ctggtccagc tcggtctggc
     2161 tgaagacctt tccggtgggg ttgtgcgggc tgttgacgat gatcgccttg gtccgcggcg
     2221 agatctgcgc gcggatctcg tcgggatcga acgggaagtc cggctccagc cggagccgga
     2281 cgaatcgcgg cacggcgccc gagagtttgg tgtccgggcc gtagttctcg tagaacggct
     2341 cgaagatgat cacttcctcc cccgggttca ccaccgcgag cagcgtggcc atcatcgcct
     2401 cggtcgcccc gcacgtgacg gtgatgtggc ggtcggggtc caccgtgatc ccgttgaacc
     2461 gccggacctt ctcggcgatg gcctgccgga acgccggggc gccccaggtg atcgcatact
     2521 ggttgtagcc ttcgcgcagg gcccgggcgg ccgcctcgac cagttcggcg ggcgcgggga
     2581 agtccgggaa cccctgggcg aggttgatcc cgccgtgctc cgccgccagg cgtgtcatct
     2641 cccggatgac cgactccgtg aagaccccca cccgtgatgc cgtcgtaatc atcgctcccc
     2701 tccgtcgcct tgcgtgcccc ggcccgcgct agtgcgggac cacccgcccg acgctgtaga
     2761 cctccgggag ctgaccgatc cgctgcatca tcgcggtcag ctgggtcacg ttccgaatgt
     2821 ccaccacgat gttgacgatg cccaccttgt ccttgcggac ccgcgcg
//
LOCUS       DASQGH010000421         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47183, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000421 DASQGH010000000
VERSION     DASQGH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47183"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VEW91_08260"
     CDS             complement(<1..971)
                     /locus_tag="VEW91_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HYM91609.1"
                     /translation="MIGLGKLGTCTAACLAARGFEVIGVDINDDSVRSINRGISPVYE
                     PRLQGFIAAAGARLRATSEYADAIKASDITFLIVPTPLEPDGQFSDKYLRDALTHLSL
                     AFGRTRKPYHLFVITSTVSPGTTDDRLIPLIERVSGKKLGRDFGVCYNPEFIALGSVI
                     RDFLNPDMVLIGESDPRAGGEVESIYRHACQNQPLIARMTVVSAEITKISLNAYVTMK
                     ISFANTLASICEAIPGADLERITEALGADRRVSPHTLKGGLPFGGPCFPRDNRAFAAF
                     AERFGVDAKLAKACDQMNGYRAERVTDFILRQLSTTSRRNVSILGLA"
     gene            complement(1154..>2875)
                     /locus_tag="VEW91_08265"
     CDS             complement(1154..>2875)
                     /locus_tag="VEW91_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91610.1"
                     /translation="RSGRLLGLVLLTCLTAQVVAVLLLVRPGAFWSPDSAVRFVQVES
                     LLRSHYRDLAVPYPAASIDPEGRYFPLGGWFHFQRTGRFYLSYLPYFSIASAPLYRLF
                     GPPGLLLIPAASVLGTVWITYGVLRRRAPAVAGAGALALGLGTPLFIYGVVFWDHSLA
                     VLLSAGALAILGVEAEAPRPGHSAAVAAAGALLAMGFWLRSEMYLLALAAGAGWVVAV
                     PRGRSKGLIALACGGALPAACLWIANARLFGSPLGWKGQDLIATRMSGAAHAAAGGWA
                     GWVSEKLGNAYYQLISPDFYAFNPPAVATGVAVGLALLLAGVLIRMGVGRRSIPAIVS
                     GTVLGAGTAVWIATGRTAVSGLLPASPLVVLALLRGSGSPWERFLWVTTGLFGAAVIA
                     TGTHGGLQWGPRYLLPILPVLVWLAASALAQARAAAFQTWPALRAAAVALLAASMLVQ
                     ASGVDQVWQAIRQNARINQWLRGIPAEIVVTPLEWLTLGAGPVYFEKQLMLVHSPVEF
                     KALVERLSAGRVGRWAYIPYSGPAFLRRSVEQWTADRPWRFRVDDDRTWNGLRFVTFA
                     GSPHEVR"
BASE COUNT          569 a          974 c          932 g          400 t
ORIGIN      
        1 taggccagcc cgaggatcga gacgttccgg cgcgaagtgg ttgagagttg gcgcaaaatg
       61 aaatcggtca cacgctcggc ccggtaccca ttcatctggt cgcaggcttt ggccaacttt
      121 gcgtccacgc caaagcgctc ggcaaaggcg gcaaacgctc gattgtccct ggggaagcac
      181 gggccgccga acgggagacc gcccttgagg gtgtggggag aaacccttcg gtccgcgccc
      241 aaggcctccg tgatccgttc gaggtccgca ccggggatcg cctcgcagat gctcgcgagg
      301 gtgttcgcaa agctgatctt catggtcacg tacgcattga ggctgatctt ggtgatttcg
      361 gcgctcacaa ccgtcatgcg tgcgatcaag ggctgattct ggcaagcgtg ccggtagatc
      421 gactccacct caccgcccgc acgggggtcg ctctccccga tcaacaccat atcgggattc
      481 aagaagtctc tgatcacact tcccaatgcg atgaactcag gattgtagca gacaccgaag
      541 tccctgccga gtttcttgcc tgaaactcgc tcgatcaggg gaatgagacg gtcgtcggtc
      601 gtgccgggcg acaccgtcga cgtaatcaca aagaggtggt acggctttcg cgtccggccg
      661 aatgccaaag agagatgcgt caacgcgtcc cgaaggtact tgtctgaaaa ctggccgtcg
      721 ggctccagcg gagtgggcac gatcagaaac gtgatgtccg aggccttgat cgcgtccgcg
      781 tactcgctcg tcgcgcgaag cctcgcaccc gccgcggcga tgaatccctg tagccgcggc
      841 tcgtagacgg gtgatatccc acggttgatt gacctcacgg agtcgtcgtt gatgtcgacc
      901 ccgatgacct caaacccacg agcggcaagg cacgcggcag tacacgtccc gagcttgccc
      961 agcccgatca ccgaaagggc gggcttcgct cgaacgtctg cagcgacgtt catgggttgt
     1021 ggtaggcgat ccgagagtgc catcgagaag cagcccccct tcgcagttct ctgccccaaa
     1081 cctccaccaa ttagactcat caagccagca cccgggggga gtcagacgct tcttgccaga
     1141 accctagtca tcttcatcga acctcgtggg ggcttccggc gaaggtgacg aaccgaagcc
     1201 cgttccacgt tcgatcgtca tcaacacgaa accgccacgg ccggtcagcc gtccactgct
     1261 cgactgaacg ccgcaagaac gcgggtcccg agtacgggat atacgcccac cgcccgaccc
     1321 gcccggccga gaggcgctcg acaagcgcct tgaactccac cgggctatgc accagcatca
     1381 gttgcttttc gaagtacacg gggccggcac ccagcgtcag ccactcgagc ggcgtcacca
     1441 cgatctcggc ggggatgccc cggagccact ggttgatgcg ggcgttttgc cggatggctt
     1501 gccacacctg gtccactccc gaggcctgga cgagcatgct ggccgccagc aacgccaccg
     1561 cagcggcccg gagggcgggc caagtctgaa aagcagcggc gcgagcctgg gctaacgccg
     1621 aggcggccag ccagacgagc acgggcagga tagggagcag gtaccggggg ccccactgga
     1681 ggcctccgtg cgtcccggtg gcgatgacgg cggcgccgaa caggccggtc gtcacccaaa
     1741 ggaaccgctc ccagggcgac cccgacccgc gaaggagcgc caggaccacc agcggtgagg
     1801 ccgggaggag cccggagacc gccgttcggc ctgtggcgat ccacacggcg gtccccgcac
     1861 cgagaacggt tcccgatacg atcgcaggga tcgagcggcg gccgacgccc attcggatga
     1921 ggaccccggc aagcagcagc gcgagcccca cggccacacc agtcgcgacc gccgggggat
     1981 tgaaggcata gaaatcgggc gatatgagct ggtaataggc gttgcccagt ttctcgctca
     2041 cccaccctgc ccagcccccc gcagccgcgt gcgcggcgcc gctcatcctg gtggcgataa
     2101 gatcctggcc tttccacccg agcggacttc caaacaagcg ggcgttggcg atccacaggc
     2161 atgcggccgg caaggcaccc ccacaggcga gagcgatcag tccctttgag cgaccgcggg
     2221 gcaccgccac cacccacccc gcccccgccg ccagcgccag gaggtacatc tcgctgcgga
     2281 gccagaaccc catcgccagg agtgcccccg cggccgccac cgccgccgag tgccccggtc
     2341 tcggggcctc ggcctccacg ccgaggatgg cgagggcgcc ggcgctcagc agcacggcca
     2401 ggctgtgatc ccagaacacc accccgtaga tgaagagcgg ggtcccgagc cccagcgcca
     2461 acgcgcccgc gcccgccacc gcgggggccc gccgacgcag cacgccgtag gtgatccaga
     2521 ccgtgccgag gaccgatgcg gcggggatga gcagcaagcc cggggggccg aagagccggt
     2581 agagcggcgc ggatgcgatc gagaaatacg gcaaatagga caggtagaat cggccggtcc
     2641 tctggaaatg gaaccacccg ccgagcggaa agtaccgccc ctcgggatcg atggacgccg
     2701 ccgggtacgg cacggcgaga tcgcggtagt gggaccggag gaggctctcg acctggacaa
     2761 agcgcaccgc cgagtcgggg gaccagaacg cgccgggccg caccagaagg agcaccgcga
     2821 cgacctgggc ggtgaggcag gtgagcaata cgagccccag gaggcgccca gatct
//
LOCUS       DASQGH010000422         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47185, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000422 DASQGH010000000
VERSION     DASQGH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47185"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VEW91_08270"
     CDS             <1..357
                     /locus_tag="VEW91_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYM91611.1"
                     /translation="GWLFEKKGLITLRKAVASEEEVLNTAIEAGAEDMKVTADEYEIT
                     TAPEEFARVKHALETAGYAPSSAEITLLPKSTVPVAGKDAQRVLRLMEALEDHDDVQH
                     VYANFDIPDEVLEQVG"
     gene            387..830
                     /locus_tag="VEW91_08275"
     CDS             387..830
                     /locus_tag="VEW91_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM91612.1"
                     /translation="MQPAFHLIWRTVQKNVEAMPEEGFELRPQGLETRSFREIAIHMA
                     NVCVTFGENVGRSTWERIIAFPPEKHTHKGPVLAALGQGGEAFLAGLSRLSDEEAARV
                     VRTPWGVEMAQGQIVAGHVPHMFYHNGQLTIYLRMQGVKPLFLAR"
     gene            915..1424
                     /locus_tag="VEW91_08280"
     CDS             915..1424
                     /locus_tag="VEW91_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYM91613.1"
                     /translation="MRILGVDPGLTWTGYGLVEVSALGPGLLDAGVIHTDGRAPLPSR
                     LHELYVRLGQVLSGGRPTVIAIEDLFAHPRFPRTAIVMGHVCGVIHLAAAQAGIPIEA
                     IPPASVKRALASSGQAGKRQMQRMVRILLALAEDPGPHVADALALALVVVSRRGLPLD
                     RSAALRGSA"
     gene            1421..2053
                     /gene="ruvA"
                     /locus_tag="VEW91_08285"
     CDS             1421..2053
                     /gene="ruvA"
                     /locus_tag="VEW91_08285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYM91614.1"
                     /translation="MIAFLRGRVVRRLEQTLVVECGGVGYEIHLPEFVRDALRGTAQV
                     PGETVEFHISYHVSANQPRPLLVGFLREIEQEFFELFITVDGLGPTKAMKAIIHPVEI
                     IADAIERKDIAFLRRLPGIGERTAGKIVATLHGKMAKYALLRTEAPQVPVEDHADFRE
                     EVIGVLTGQLGHRTAEARRMVERALERNPAIASAEQLFQEVYRAERGAPV"
BASE COUNT          350 a          689 c          796 g          320 t
ORIGIN      
        1 gggtggttgt tcgagaaaaa gggtctgatc accctccgga aggcagtggc gtccgaggaa
       61 gaggtcctca atacggcgat cgaagccggc gccgaggaca tgaaggtgac cgcggacgaa
      121 tacgagatca cgaccgcgcc ggaggaattc gccagggtca agcacgcgct ggagaccgcc
      181 gggtacgcgc cgagctccgc ggaaatcacg ctcctcccca agtcgaccgt gccggtggcg
      241 ggcaaggacg cccagcgcgt gctgcggctc atggaggcgc ttgaggatca cgacgatgtc
      301 cagcacgtgt acgccaactt cgacatcccc gacgaagtgc tcgaacaggt cgggtgaagg
      361 gagccggcag gggagtccgt gagagcgtgc agcccgcatt ccacctgatc tggcgaaccg
      421 tgcagaagaa cgtcgaggcg atgcccgagg aggggttcga gctccggccg caggggctcg
      481 agacccggtc gtttcgcgag atcgcgattc acatggcgaa tgtgtgcgtg acgttcgggg
      541 aaaacgtcgg ccggtccacc tgggagcgca tcatcgcctt tcctcccgag aagcacaccc
      601 ataaagggcc cgtgcttgcc gcccttgggc agggtggaga ggcgttcctc gcggggctga
      661 gccgcctctc agacgaagag gcggcccggg tcgtccggac gccgtggggg gtggagatgg
      721 cccaggggca gatcgtcgcg gggcacgtgc cgcacatgtt ctaccacaac ggccagctca
      781 ccatctacct ccggatgcag ggggtcaagc cgctgttcct cgccaggtag gccgacagga
      841 accccagcag aaatcggcga acagtggttc gggtcggtag ccgacctttc accgccgaat
      901 cacacggagt tgcgatgcgc atcctcgggg tcgacccagg cctcacctgg accggatacg
      961 ggctcgttga ggtgtcggcg ctgggaccgg gcctcctcga cgccggggtc atccacaccg
     1021 acggccgggc accgctcccg agccggcttc acgagctgta cgtgcggctc ggccaggtcc
     1081 tctccggtgg gcgcccgacg gtgatcgcga tcgaggacct gttcgcccac ccccggtttc
     1141 cccgcacggc gatcgtcatg gggcacgtgt gcggggtcat ccatctggcg gccgcacagg
     1201 cggggatccc gatcgaggcg attcccccgg cgtcggtgaa gcgggcgctg gcctcctccg
     1261 gccaggccgg taaacgccag atgcagcgga tggtccggat cctgctggcg ctcgcggagg
     1321 acccgggtcc ccacgtggcc gatgcgctcg cgctggcgct ggtcgtggta tcgcgccggg
     1381 ggcttccgct cgaccgctcc gcggcgcttc ggggctcggc gtgattgcgt tcctccgggg
     1441 gcgggtggtg cggcgcctcg agcagacgct cgtggtcgag tgcggcgggg tggggtacga
     1501 aatccacctg ccggagtttg ttcgggacgc cctccggggc acggcgcagg tgccggggga
     1561 gacggtcgag ttccacatct cgtaccacgt ctccgcgaac caaccgcggc cgctgctcgt
     1621 ggggtttctg cgggagatcg agcaggagtt tttcgagctg ttcatcacgg tggacggact
     1681 ggggcccacg aaagcgatga aggcgatcat ccacccggtc gagatcatcg ccgacgccat
     1741 cgagcgaaag gacatcgcgt ttctccgccg gctgcccggg atcggcgagc ggacggccgg
     1801 gaagatcgtg gcgaccctcc acgggaagat ggccaagtac gccttgctcc ggaccgaggc
     1861 tccgcaggtg ccggtggagg accacgcaga cttccgcgag gaggtcatcg gcgtgctgac
     1921 cgggcagctg ggccaccgga cggccgaagc ccgccggatg gtcgagcgcg cgctcgagcg
     1981 gaatcccgcg atcgcgtcgg ccgagcagct gttccaagag gtctaccgcg ccgagcgggg
     2041 ggcgccggtg tgacggggga gcgcgtgccc aaaccggtgg gagccccggg cgggagtccc
     2101 gcgggggccg cagacgcggc gcggggggag gaggcccagt tctttcacag cctgc
//
LOCUS       DASQGH010000423         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18871, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000423 DASQGH010000000
VERSION     DASQGH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18871"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..637
                     /locus_tag="VEW91_08290"
     CDS             74..637
                     /locus_tag="VEW91_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYM91615.1"
                     /translation="MTASPHRAVFFDRDGVLNEAIVKEGKPYSPAGPVELTICSGVPD
                     ALRVLKEAGFLLVVVTNQPDVARGTQRRDVVEAINARLLAALPLDAIYVCYHADRDGC
                     PCRKPRPGLLLRAATEYAIDLPRSFMIGDRWRDVEAGRQAGCVTIRLDRQYAQMEPES
                     SPDYQAYSLPDAASWILARDRVAGGIQ"
     gene            634..1374
                     /locus_tag="VEW91_08295"
     CDS             634..1374
                     /locus_tag="VEW91_08295"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HYM91616.1"
                     /translation="MRPVSDLRVKIFADGADLQGMREMARQPWIKGFTTNPTLMRKAG
                     VPDYRAFARDVLATIPDRPISFEVFSDEFHEMVHQAREIATWGKNVYVKIPVTNTRRE
                     PSTRVIRDLSSSGVKVNVTALLALDQVRDVAAALAGGAPSYISVFAGRIADTGRDPVP
                     LMAAACEIVAVFPNIELIWASPREVLNIFQADAIGCHIITVTNDILKKLDTVGKDLHD
                     FSLETVKTFRGDAIQAGYMLPIRTGSEA"
     gene            1472..>2223
                     /locus_tag="VEW91_08300"
     CDS             1472..>2223
                     /locus_tag="VEW91_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYM91617.1"
                     /translation="MDLLLRKGYGVRILDSLQPRVHPQGKPSFLPPEAEFIHGDVANR
                     EDLGRALQGVDYVFHLAAYQDYMPDFSQFIRTNTESAALMFELAIANRKRYPVRKIVF
                     ASSQSVSGEGAYLCRTCADGVRVPSIQEQLIAGVSPIDQLPPKAVHVPGPRPIEQLKR
                     GDWEIRCPVCHGNMQPLLIQETTISPSTAYGISKYAIELLADRLGRRYGIPAVCLRYT
                     YAQGPRNSFYNAYSGVTRRFALRILHSLPPVV"
BASE COUNT          396 a          694 c          729 g          404 t
ORIGIN      
        1 agtcggtcct ctggcatctc atggtgtcgc acccgaagct gaagcggtcc cagacgaagt
       61 gggagtcgca gcgttgacag catcgccgca ccgcgccgtg ttcttcgatc gggacggcgt
      121 gctgaacgag gcgatcgtga aggaggggaa gccgtattcg cccgccgggc cggtagagtt
      181 gaccatctgc agcggtgtgc cggacgcgct ccgggtgttg aaggaggccg ggtttcttct
      241 ggtggtcgtg acaaaccagc cggacgtggc tcgggggacg cagcggcgcg atgtcgtcga
      301 agcgatcaac gccaggctcc ttgccgcgtt gccgctggac gcgatctacg tatgctatca
      361 cgccgaccgg gatggctgtc cgtgccgcaa gccgcgcccg ggcctcctgc tccgggcggc
      421 gaccgagtac gcgattgatt tgccgaggag tttcatgatc ggagatcggt ggagagatgt
      481 ggaggccggc cgccaggcgg ggtgtgtgac catccggctg gaccggcagt atgcgcagat
      541 ggagccagag agctcaccgg attatcaggc gtattcgctc ccagacgcgg cctcgtggat
      601 tctcgcgcga gaccgcgttg caggagggat ccagtgaggc cggtgtctga cctgcgggtg
      661 aagatcttcg cggacggggc ggacctgcag gggatgcgcg agatggcccg ccagccgtgg
      721 atcaaggggt tcacgaccaa ccccaccctc atgcgaaagg ccggggtgcc cgactaccgg
      781 gccttcgccc gcgacgtgct cgccacgatt cccgatcggc cgatctcgtt tgaggtgttc
      841 tccgatgagt ttcatgagat ggtgcaccag gcgcgagaga tcgcgacgtg gggtaagaac
      901 gtctacgtca agatcccggt gaccaacacc cgtcgggagc catcgaccag ggtgattcgc
      961 gacctgtcgt cctcgggcgt caaggtcaat gtgaccgcgc tcttggcgct cgaccaggtg
     1021 cgcgacgtgg ccgcagcgct cgccgggggc gcaccatcct atatctccgt gtttgccggt
     1081 cgaatcgcgg acacgggacg ggacccggtt ccactcatgg ccgcagcctg cgagatcgtg
     1141 gcggtgttcc caaacatcga gctcatctgg gcgagccccc gcgaggtctt gaacattttt
     1201 caggccgatg caatcggctg ccacatcatc accgtcacga acgacatcct caagaagttg
     1261 gacacggtgg ggaaagacct gcacgatttt tcgctcgaga ccgtgaagac gttccgaggt
     1321 gacgcaatcc aagccggcta catgctgccg attcggaccg gatcggaagc gtaacagatg
     1381 agcagagccg ataacgctcg gacaggggtg cggtaggctc atgcctttat gcctcgtgac
     1441 tggtggcgcc gggttcatag gctctcacac ggtggaccta ctgctgcgca aaggatacgg
     1501 ggttcgtatc cttgacagct tgcaaccccg cgtgcatcct caggggaagc ccagcttcct
     1561 cccgccggag gcggaattca tccacggcga tgtcgccaac cgggaagacc tcggtcgggc
     1621 gctgcagggg gtcgactacg tcttccacct cgcagcctac caggactaca tgccggattt
     1681 ctcccagttc atccggacga ataccgagag tgcggcgctc atgtttgaac tggcgatcgc
     1741 caaccggaag cgctatccgg ttcgcaagat cgtcttcgcc tcctcccagt cggtctcggg
     1801 cgaaggggca tatctctgcc ggacctgcgc ggacggggtg cgcgtgccga gcatccaaga
     1861 acagctcatc gccggcgtgt cgccgatcga ccagctgccg ccgaaggcgg tccacgtccc
     1921 tgggccccgg cccatcgagc agctgaagcg cggggactgg gagatccgct gccccgtctg
     1981 ccacgggaac atgcagcccc tgctgattca ggaaaccacg attagcccga gcacggccta
     2041 cgggatttcc aagtacgcga tcgaactcct ggccgaccgg ttgggacggc ggtacgggat
     2101 tcccgcggtc tgcctgcggt atacgtacgc ccaagggccg cgcaactcgt tctacaatgc
     2161 ctactcgggg gtcacgcgtc gctttgctct tcgcatcttg cacagcctcc ccccagtcgt
     2221 cta
//
LOCUS       DASQGH010000424         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_15959, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000424 DASQGH010000000
VERSION     DASQGH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15959"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VEW91_08305"
     CDS             complement(<1..243)
                     /locus_tag="VEW91_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM91618.1"
                     /translation="MQRRAGWPAGEGASPSTTRKIAEGIPARVANRQIFGMAYEVIVV
                     GAGPAGAEAARAAARGGLRALLVEEHPTVGVPSHCTG"
     gene            204..1751
                     /locus_tag="VEW91_08310"
     CDS             204..1751
                     /locus_tag="VEW91_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91619.1"
                     /translation="MRLHRLATLLVAACMLAAPAEGGAVPAQTGAQDVLRDLIDVPGA
                     AEAHTPAPLNRGVAFRYSVPSTQAPSSVLIFVPGLNSGPNTADILARTLVASQGPALQ
                     VWVVAVRSTLIQDRRGVDAALAYRHPDFALGYYYGTLEIDGHTFHRLKGPEVPYMAYW
                     GLDVHLRDIRAIVREVHSRYPAARVVLGGHSLGGILAAAYAGYDFAQIPGASRAVPPP
                     PADVGGGDLDGLLFLDGLPVRIPLRVTPDRYLHGFGVPILGHIPGVEALTNPDPKKRE
                     APFTQTSKLARTEDSILLDVVSVYALLRPEAASYFPFFPRRGLKITNDALAAAIFSDQ
                     MQPDALVRAGVELPLGVFDRVSDPAYVNRRGLLDLQSGQPAPGEPLIRLPRDPDPRPR
                     VPIRTLLEAILQPGGDFTVWYFPWRLVLDVALVAAIDPHDQFARRYASLTHIGDTRLP
                     MLIVGAGHGLVRSSGATEYYRQLVATPPGQVTVKIFPDYSHLDIEDADPNPVVPMILA
                     WLRTVVH"
     gene            1783..2313
                     /locus_tag="VEW91_08315"
     CDS             1783..2313
                     /locus_tag="VEW91_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYM91620.1"
                     /translation="MRAAVWVTIAGVGAALASAGPAPAGAPCSVPLFPYRTGILVLSE
                     GGRMTRVRVQLADTPDRLEVGLMCRPALDPDAGMLFVFPTSTRTAFWMKNTLIPLAIA
                     FIDSDWHIVRIMEMAVAPDPAEGPFAFYEPDKPFRYALEVNAGFFAKHEISERAEVRF
                     IPPEGGGPPTFAPAAP"
     gene            2481..>2704
                     /locus_tag="VEW91_08320"
     CDS             2481..>2704
                     /locus_tag="VEW91_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91621.1"
                     /translation="MSESGGTLPAFGGVESTLLWLVFAAGACALGYGWWLIGRVLRRS
                     PGPASMQAVAAAIQEGAQAYLGRQAGTMSL"
BASE COUNT          377 a          980 c          905 g          442 t
ORIGIN      
        1 cccggtgcag tggctcggaa ccccgacggt ggggtgctcc tcgaccagca gcgcccggag
       61 ccccccgcgg gcggcggcgc gggccgcctc cgctcccgcg gggcccgcgc cgaccacgat
      121 gacctcgtac gccatcccga agatctgacg gttcgcgact cgggcgggga ttccctctgc
      181 gattttcctc gttgtagaag gagatgcgcc ttcaccggct ggccaccctg ctcgtcgctg
      241 catgcatgct tgccgccccc gcggaaggcg gggcagtccc ggcgcagaca ggcgcccagg
      301 atgtcttgcg ggacctcatc gatgtgcccg gagccgccga ggcgcatacc ccggccccgc
      361 tgaaccgggg ggtggccttc cgctactccg ttccgagcac gcaggccccg tcatcggtcc
      421 tcatctttgt cccggggttg aactcgggac cgaacaccgc cgatattctc gcgcggactc
      481 tggtcgcatc ccagggaccg gcgctccagg tctgggtcgt ggccgtgcgc tcgaccctga
      541 tccaggaccg caggggagtc gacgcggcgc tcgcgtaccg gcacccggat ttcgcgctcg
      601 ggtactacta cggcacgctg gagatcgacg ggcacacctt ccaccggctc aaaggccctg
      661 aggtgcccta catggcctac tgggggttgg acgtccacct gcgcgacatc cgggcgatcg
      721 tccgcgaggt ccattcacgc tacccggccg cccgggtggt cctcgggggc cattccctcg
      781 gcgggatcct cgcggcggcg tacgccggct acgatttcgc ccagatcccg ggggcctccc
      841 gggcggtccc gccgcccccg gcggacgttg gcggcgggga cctcgatggg ctgctgtttc
      901 tcgacggcct gcccgtgagg atccccctgc gggtaacgcc cgaccgatac ctccacggct
      961 ttggcgttcc gatcctcggc catatccccg gcgtcgaggc cctcaccaac ccggatccca
     1021 agaagcgcga agcgccgttc acccagacca gcaagctcgc gcgcacggag gacagcatcc
     1081 tgctggatgt cgtgagcgtg tacgcgctcc tgcgaccgga ggcggcgagc tactttccct
     1141 tcttccccag acggggcctc aagatcacca acgacgcgct cgcggccgcg atcttctccg
     1201 accagatgca gccggacgcc cttgtccgcg ccggggtcga actgcccctg ggggtcttcg
     1261 accgggtgag tgaccctgcg tatgtcaatc gccgcgggct gctggatctc cagagcgggc
     1321 agccggcgcc cggagaacca ctgatccgcc tcccgcgcga tccggacccg cgcccccggg
     1381 ttccgatccg cacgctgctc gaggcgatcc tccaacccgg cggggatttc acggtgtggt
     1441 acttcccctg gcggcttgtg ctcgacgtgg ccctcgtcgc ggccatcgat ccccacgacc
     1501 agtttgccag gcgctacgcg agcctgacgc acatcggcga cacccggctg ccgatgctga
     1561 tcgtcggcgc cgggcacggc ctcgtccgct cctcgggcgc gaccgagtac taccggcagc
     1621 ttgtcgcaac cccccccggc caggtgacgg tgaagatctt cccggactac tcgcacctgg
     1681 acatcgagga cgcagacccc aacccggtgg tgccgatgat tctcgcctgg ctcaggacag
     1741 tggtacacta gcgggagacc tgggaggggg aaggcgggaa ggatgcgggc ggccgtctgg
     1801 gtcacgatcg caggagtcgg cgccgcgctg gcgagcgccg gaccggcccc tgccggcgcc
     1861 ccctgctccg tcccgctgtt tccgtaccgc accgggatcc tggtcctgag cgagggcgga
     1921 cgcatgacgc gcgtccgtgt gcagctcgcc gacaccccgg accgcctgga ggtcggcctc
     1981 atgtgccggc cggcgctgga tcccgacgcg gggatgctgt ttgtgtttcc cacctccacg
     2041 cgcacggcgt tctggatgaa gaacaccctg atccccctcg cgatcgcgtt catcgactcc
     2101 gactggcaca tcgtgcggat catggagatg gcggtggcgc ccgacccggc cgaggggccg
     2161 ttcgccttct acgaacccga caagcccttc cgctacgccc tggaagtgaa cgcggggttc
     2221 ttcgccaagc acgagatctc cgaacgggcg gaggttcggt tcatcccgcc ggagggcggg
     2281 ggccccccca ccttcgcgcc cgcggcgcca tagccgttca cgccccaggg accgggaaac
     2341 cggcgctcca ctcgacgaat accgcgatct agctcggaaa cacgctggtt ttcggctgaa
     2401 tccttttgct ttctgttgag tcgcagggca gcgcctgcta tgatggaaac gccccgaggg
     2461 aatcgcaagg aggaatgcgc gtgagcgaat cgggcggcac ccttccggcg tttggggggg
     2521 tcgagtcgac gctcctatgg ctggtgttcg ccgcgggcgc ctgtgcgctt ggctacgggt
     2581 ggtggctcat cggccgcgtg ctccggcgga gtcccgggcc ggcatcgatg caggcggtcg
     2641 ccgccgcgat ccaggagggc gcccaggcct atctgggccg gcaggccggg acgatgtccc
     2701 tgtt
//
LOCUS       DASQGH010000425         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1788, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000425 DASQGH010000000
VERSION     DASQGH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..850
                     /locus_tag="VEW91_08325"
     CDS             443..850
                     /locus_tag="VEW91_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYM91622.1"
                     /translation="MTLTDTGPLVALLDRDEPDHTKCAEQLRYLRAPMLATWPVFTEA
                     MYLIGDSLGWRGQEALWRILRRGDLHVAETRASMVDRMERLMLKYRDVPMDLADASLV
                     AVAEERGLTKVFTLDRDFHVYRVLGRRPFVIVP"
     gene            complement(972..1883)
                     /locus_tag="VEW91_08330"
     CDS             complement(972..1883)
                     /locus_tag="VEW91_08330"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HYM91623.1"
                     /translation="MIPLLNPFTRPGRWYRGVFHCHSTNSDGNRSVESVTAWYADAGY
                     DVVAITDHNQLTPPHLPRAHRLLLVPGTEVDVGRTRLGESYHIVGIGLGEMIEIPRDV
                     ASRQRLAVQDVVGALRRAGAVVFIAHPYWSGLVADDLLPLDGVAGIEVFNANCEVDIG
                     KGYSGVHWDDCLSRGKLLWGAATDDAHWRLPDYGRAWTMVRAEERTREAVVGALAGGA
                     FYASTGAVLEDVTFDGAVATVRVGAPGASEVRFICNWRWGHRVVADGAPLETATYALR
                     GREKYVRVEVTTPSGHRAWTNPLFLER"
     gene            complement(1880..3499)
                     /gene="glpK"
                     /locus_tag="VEW91_08335"
     CDS             complement(1880..3499)
                     /gene="glpK"
                     /locus_tag="VEW91_08335"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HYM91624.1"
                     /translation="MSTVPAGVEGAGSGPYVAAIDQGTTGTRCIVFDTSGKPRANAYR
                     EHAQLTPEPGWVEHDAEEIWGATAAVVRDALAQAGLVGPRARLLAGVGVTNQRETTVL
                     WDRRTGRPVYRAIVWQDTRTRTTCQALTGRGVEPLVRARTGLPIATYFSALKAAWVLD
                     HVPGVRAHAERGDVCFGTIDSWVIWWLTGGPAGGVHVTDPTNASRTLLMDLRTQTWDD
                     ELLELFGLPRAMLPEIRCSSERYATTVPDGPFGAAVPVCGDLGDQQAALVGQTCYRPG
                     DAKNTYGTGCFLLEHTGERPIPSRSGLLTTVAYALPNRVAFALEGSIAIAGAAVQWLR
                     DNLGLIRTAEETEAIAGSVEDSGGAYFVPAFSGLFAPHWDMRARGVIAGLTRFVTRAH
                     LVRATLEAICYQTREALEAMEHDVGIHLSALRVDGGASRNNLLMQLQADILGVPVIRP
                     TVSETTALGAAYLAGLAAGVWDSLEGLRAHWKVDRRFDPTWPVDRREAGYAGWRRAVE
                     RARDWIPPDPAALAREGAPSPASAAPKGEDA"
     gene            complement(3496..4290)
                     /locus_tag="VEW91_08340"
     CDS             complement(3496..4290)
                     /locus_tag="VEW91_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HYM91625.1"
                     /translation="MSRTAGTGASRPAEGRGHRFLGTGRTLGVAHRGASRYAPENTLA
                     AFRLALEHGAPAVECDVRRTQDGHLVVMHDPTVDRTTDGHGLVGSLALEALRRLDAGR
                     WFGPEFAGARIPLLEEVLDAVRGRALIKVEIKNDPTAQEGIEQQVVDTIRRRGMEDEA
                     FVMSFDHQVVRRVREAAPRIATGVLYSARLVDPVSAARAAAADALCVDWGYRDRGLVA
                     EGHRAGLGVFVWTVDDEPALRRCQELGVDGITSGDTRMIGRLLGGA"
     gene            complement(4287..4814)
                     /locus_tag="VEW91_08345"
     CDS             complement(4287..4814)
                     /locus_tag="VEW91_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HYM91626.1"
                     /translation="MFVIGEVSFREELLEAGFVLTDDPRKVDVVVAAFDRGFDYHKLQ
                     IAFDAIRSGARFVATNRDPYCPVPGGGLPDCAAVIAAIEASTGGRVEEVVGKPSAVMA
                     HVVLDRLGLPPDRAIMVGDRLETDIAMGVLAGMATALVLTGAARRSDLVRASVRPQFV
                     LEDLRGLLSQDARER"
     gene            complement(4754..5098)
                     /locus_tag="VEW91_08350"
     CDS             complement(4754..5098)
                     /locus_tag="VEW91_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91627.1"
                     /translation="MTGGDARPAAYEGYALDLDGTVYLGEQLLPGARETIARLRGAGR
                     RVVFLSNNPLMSSVGYAARLTRLGVPTGPDDVVNSSQVMVDYCGPAFPDGGCSSSGRC
                     PFGRNCSRRGSC"
     gene            5236..>5589
                     /locus_tag="VEW91_08355"
     CDS             5236..>5589
                     /locus_tag="VEW91_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HYM91628.1"
                     /translation="MPGRHFLQIPGPTNVPDRILRAMDRPVPDHRGPELPALVEESLA
                     GLRQVFQTTRGEIVLYPGSGTAAWEASLVNTVSPGDRVLAFNIGHFSHLYAECARQHG
                     IVVDEIDLPWGDGVPP"
BASE COUNT          872 a         2042 c         1863 g          812 t
ORIGIN      
        1 cacgatgccg gaggcgatca ccgaggcgtt cgcgcgggca ttgaccgagg cggcgggata
       61 ggcgcacagc gcgcagaagg tttcacgccc ccgccgagac gatcccagga cgcacagaat
      121 ccacttgccg tttgcgcccg ccccgttcct gtgatacggt tgaggtaaat tgtaatacac
      181 gtggagggcg atcgatggcg aagagcttcc gcctggatcc cgcgttggag gcacggttgg
      241 agcgggctgc acaggccgac ggaatatcca cgtccgaaac catccggcga gcggtcgtac
      301 ggtactgtga agaggtgcta ggcaataccc tgcaccccag attggccgac gtgatcggaa
      361 taggccatgg cggtgggggc cgagcgcgca gaagtggtga cgcgctccga cgggtcctcc
      421 gtcggaagca ccgcaaatcc acgtgacgct caccgacacg ggccccctgg tcgctctcct
      481 ggatcgggat gagcccgacc acaccaagtg tgccgagcag cttcgttacc tgcgtgcccc
      541 gatgcttgcg acgtggcctg tcttcacgga ggcgatgtat ctcattggtg acagtctcgg
      601 gtggcgcggg caggaggcgc tgtggcggat cctccggcga ggcgatcttc atgtggcgga
      661 gacgcgggcg tctatggtcg atcggatgga gcgactcatg ctaaaatatc gcgacgtgcc
      721 catggatctc gcagacgcct ccctcgttgc cgtcgcagaa gagcgtgggc tcacgaaggt
      781 ctttaccctt gaccgcgatt tccacgtcta tcgcgtgctc ggccgccggc cgtttgtcat
      841 cgtcccatag aacctacctg gtcatcgcgc gaacacggtg gtctgcgcta tgccgagacc
      901 gatttttccg cctgacggag gcgcctggaa gatggcctgc tcccgcgccg tcgcgcgccc
      961 ctccaccgcg gttacctctc caagaacagc gggttcgtcc aggcgcggtg cccggaaggc
     1021 gttgtgactt ccacccggac gtacttctcc cgaccacgaa gcgcgtaggt cgcggtctcg
     1081 agaggcgccc cgtccgcgac cacgcggtgc ccccaccgcc agttgcagat gaaccggacc
     1141 tccgatgccc cgggcgcccc cacccgcacc gtggcaacgg cgccatcgaa ggtcacatct
     1201 tcgagcacgg cccccgttga ggcgtagaac gccccgccgg cgagcgcccc gacgaccgcc
     1261 tcgcgggtcc gctcctccgc ccgcaccatc gtccacgccc ggccatagtc cggcaaccgc
     1321 cagtgggcat catcggtcgc cgcgccccag agcagcttcc cgcggctgag gcagtcatcc
     1381 cagtggacgc cactgtaccc tttcccgata tcgacctcgc agttggcgtt gaacacctcg
     1441 atgccggcga ccccgtccag cggcagcagg tcgtcggcga cgaggccgct ccagtacgga
     1501 tgggcgatga acaccaccgc gccggcccgg cggagggcgc cgacgacgtc ctggaccgcc
     1561 aggcgctgcc gggaggccac atcgcgcggg atctcgatca tctccccgag tccaatcccc
     1621 acgatgtgat acgattcccc caaccgcgtg cggccgacgt cgacctccgt gcccgggacg
     1681 agcagcagcc ggtgcgcgcg gggcagatgg ggcggggtca gctggttgtg gtccgtgatc
     1741 gccacgacgt cgtatcccgc gtccgcatac caggcggtca ccgactctac cgagcggttt
     1801 ccgtctgagt tcgtgctgtg gcagtgaaac acgccccggt accaccgtcc ggggcgggtg
     1861 aagggattca gcaacggaat cacgcgtctt cccccttcgg ggcggcggag gcgggtgacg
     1921 gggccccctc ccgcgcaagc gcggccgggt ccggcgggat ccagtcccgc gcgcgctcca
     1981 ccgcgcgcct ccagccggcg tatcccgctt cccgcctgtc gaccggccac gtggggtcga
     2041 accgccggtc caccttccag tgtgcgcgca ggccctcgag cgagtcccag acgcctgcgg
     2101 cgagcccggc gaggtaggcg gcgcccagcg ccgtggtctc gctcaccgtg gggcggatca
     2161 ccggcacccc gaggatgtct gcctggagct gcatgaggag gttgttgcgc gacgcgccgc
     2221 cgtcgaccct cagggccgag aggtggatac cgacgtcgtg ctccatggcc tcgagggcct
     2281 cccgggtctg atagcagatc gcttcgagcg tcgcccggac caggtgcgcc cgggtgacga
     2341 aacgggtcag gcccgcgatc acgccgcggg cacgcatatc ccaatgcggc gcgaagagac
     2401 cggagaacgc cgggacgaag taggcgccgc cggagtcttc gaccgaaccc gcgatggcct
     2461 cggtctcctc ggcggtccgg atgagcccaa ggttgtccct cagccactgc accgccgcgc
     2521 cggcgatcgc aatcgatccc tccagcgcaa acgccacgcg gttcggaagc gcgtaggcga
     2581 ccgtggtcaa caacccgctc cggctcggga tcggccgctc gccggtgtgt tccagcagga
     2641 agcacccggt gccgtaggtg ttcttggcgt cgcctggacg gtagcaggtc tggcccacga
     2701 gcgccgcctg ctggtcgccc aggtcgccgc acaccggcac cgccgcaccg aacggcccgt
     2761 ccgggaccgt ggtcgcgtac cgctcgctgc tacagcggat ctcaggcagc atcgcacgcg
     2821 gcaggccgaa gagctccagc agctcgtcgt cccaggtctg cgtcctgagg tccatgagta
     2881 gcgtccgtga ggcgttcgtg gggtcggtca cgtgcacccc gcccgccggc ccgccggtga
     2941 gccaccagat cacccaggag tcgatcgtgc cgaagcagac gtcgccgcgc tcggcgtggg
     3001 cccggacccc cggcacgtgg tccagcaccc acgccgcctt gagcgccgaa aaataggtgg
     3061 cgatcggcag ccccgtccgc gcccggacca ggggttccac gccccggccg gtaagcgcct
     3121 ggcaggtagt gcgggttcgt gtgtcctgcc agacgatcgc ccggtagacg ggcctcccgg
     3181 tccggcggtc ccagagcacc gtggtctccc gctggttggt cacccccacc cccgcgagca
     3241 ggcgggcgcg ggggccgacg agaccggcct gggccagggc gtcgcggacg acggccgcgg
     3301 tggcccccca gatctcttcg gcatcgtgct ccacccatcc gggctccggc gtcagctggg
     3361 cgtgctccct gtacgcgttc gcgcgcggct tgccggaggt gtcgaagacg atgcagcggg
     3421 tgccggtcgt cccctggtcg atcgctgcga catagggccc cgagcccgcg ccctccaccc
     3481 cggccgggac ggtgctcacg cgccacccaa caggcgcccg atcatccggg tgtcgccgga
     3541 ggtgattcca tcgaccccca actcctggca gcgcctcaag gccggttcgt cgtcgacggt
     3601 ccacacgaac acaccaagcc ccgccctgtg accctcggcg acaaggcccc gatcgcggta
     3661 gccccagtcc acgcacaacg cgtctgcggc ggcggcccgc gcggctgaga ccggatcgac
     3721 gagccgggcg gagtagagga cgccggtcgc gatccgcggc gccgcctcgc ggacgcgcct
     3781 cacgacctgg tggtcgaagg acatcacgaa ggcctcgtcc tccatgcccc ggcgccggat
     3841 ggtgtcgacc acctgctgct cgatcccttc ctgcgccgtg ggatcgtttt tgatctcgac
     3901 cttgatcaac gcccgcccgc ggacggcgtc gagcacctcc tcgagcaacg gaatccgtgc
     3961 gccggcaaac tcgggcccga accagcgtcc tgcgtcgagg cgccgcagcg cctccagggc
     4021 cagcgatccg accaggccgt ggccgtccgt cgtccggtcc acggtggggt cgtgcatgac
     4081 gaccaggtgg ccgtcttgcg tgcggcgcac atcgcactcg acggccggcg ccccgtgctc
     4141 gagcgccagc cgaaacgccg ccagcgtgtt ctcgggcgcg taccgggagg ccccgcggtg
     4201 ggccaccccg agggtccggc ccgtccccag gaaccggtga ccgcgtccct cggccggccg
     4261 gctggcgccg gtcccagcgg tcctgctcat cgctctcgcg cgtcttgcga gagcagcccc
     4321 cggaggtcct ccaggacgaa ctgcggccga accgatgccc ggacgagatc cgaccggcgc
     4381 gccgccccgg tgagcaccag cgccgtggcc atcccggcga gcacgcccat cgcgatgtcg
     4441 gtctccagcc gatcgcccac catgatggcg cggtccggtg gcagcccgag gcggtcgagc
     4501 accacgtggg ccatcaccgc cgagggcttg cccacgacct cctcgaccct cccccccgtc
     4561 gatgcttcaa tggccgcgat gacggcggcg cagtccggaa gcccgccccc cgggaccgga
     4621 cagtacggat cccggttggt ggcaacgaac cgggcgccgg agcggatcgc gtcaaaagcg
     4681 atctggagct tgtgatagtc gaacccgcga tcgaatgcgg ccacgaccac atcgaccttt
     4741 ctcgggtcgt cggtcaacac gaaccccgcc tcgagcagtt cctcccgaaa ggacacctcc
     4801 ccgatgacga acaaccgccg tccgggaaag cgggcccgca gtaatcgacc atgacctgtg
     4861 aggagttcac gacatcatcg ggtcccgtcg gcacgcccag ccgggtcagt cgggcggcat
     4921 accccacgct cgacatgagc gggttgttcg aaagaaacac gacccggcgg cccgccccgc
     4981 gcagacgggc aatcgtctcg cgtgcgcccg gaagcaactg ctcgcccaag tataccgttc
     5041 cgtcaagatc cagcgcgtac ccctcgtacg ccgcggggcg cgcatcgccg ccggtcacgc
     5101 gggtggtttc gctgcctggg accccgaacc cctgcgggtg gggaagcccg ttcgggtccc
     5161 cggccgaggc gacggcggag ggaaaggccg gacaccctcc gaagaccccg tggacgctac
     5221 aatcgaaggt gccgcatgcc cggccgacac tttctgcaga ttcccggccc gaccaacgtc
     5281 ccagaccgaa tcctacgcgc gatggaccgt cccgtgcccg accaccgggg acccgaactg
     5341 ccggccctgg tggaagagag cctggcgggg ctccggcagg tcttccagac gacgcgcggg
     5401 gagatcgtcc tgtaccccgg gtcgggcacc gcggcgtggg aggcatcgct ggtgaacacg
     5461 gtgagcccgg gcgaccgcgt gctggcgttc aacatcggtc acttcagcca cctgtacgcg
     5521 gagtgcgcgc ggcagcatgg gatcgtcgtc gatgagatcg atctgccatg gggagacggc
     5581 gtcccccct
//
LOCUS       DASQGH010000426         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7635, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000426 DASQGH010000000
VERSION     DASQGH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7635"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VEW91_08360"
     CDS             <1..1060
                     /locus_tag="VEW91_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91629.1"
                     /translation="GRPPRGRPARCMTAFIVRRLLLLPLVALGVSMLIFSLLQLLTPG
                     MRASLYITDPKQLSALPEIIRKYHLDQPVYVQYWDWLRQVLHGDLGWSETAREPVADA
                     IRGFFPATLELGIYAFACILGFGIWLGTLAAVHKDRIIDHLSRVASISGNSLPTFVWG
                     LFLLMVFYGQLGWFPPGRLSLDADQFVRTARFHAYTGLMTVDAALNRQWWVFWDAAKH
                     LVLPVATLTYYLTAVLVRITRSSMLDTLRTDYVRTARAKGLADKVVVDKHARRNALIP
                     VITLGSLLFVGLLSGVVITETVFNYPGIGRWGVGASQQLDIPAVTGFALIFAGLLVIG
                     NLCADVLYAFVDPRIRLQ"
     gene            1057..2010
                     /locus_tag="VEW91_08365"
     CDS             1057..2010
                     /locus_tag="VEW91_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91630.1"
                     /translation="MSAVRSSAPVIPLPRVPAHLPKVETGWHRTVRRFRANPMSMLGL
                     AIVAFFVALALLAPFLAHPTERNPYLIPHEGYASEPRPPSPAHPFGTTEEQFDLYYGV
                     IWGARTAFKVALIVVASAVAVALFLGSLSGFYGGWVDEAVMRVTDIFLAFPGLILAVV
                     IVAVLGQSVQNAVIAIAVVEWPTYTRLLRGEFLRVRDMEYVQAARALGSGDLRIVARH
                     LIPNTVYPILILASLSMGGIVITFAALSFLGLGAPPGYADWGQLISLSHNWIAGTPGH
                     AFTFWYTLVVPGAAIFLFVLGWNLLGDAFRDIFDPRLQGSR"
BASE COUNT          311 a          803 c          752 g          418 t
ORIGIN      
        1 gggccggccc ccgcggggcc ggcccgctcg ctgcatgacc gcgttcatcg tccgccggct
       61 gctcctcctc ccgctggtcg cgctcggggt gtcgatgctg atcttctccc tgctccagct
      121 cctcacgccc ggcatgcggg cgagcttgta catcaccgac cccaagcagc tcagcgcgct
      181 cccggagatc atccggaagt accacctcga tcagccggtg tacgtgcagt actgggactg
      241 gctgaggcag gtgctccacg gggacctcgg gtggtctgag accgcccggg agccggtcgc
      301 cgacgcgatc cgggggttct tccccgccac gctggaactg gggatctacg cgttcgcatg
      361 catcctcgga ttcgggatct ggctcgggac gcttgccgcg gtgcacaaag accggatcat
      421 cgaccatctc agccgcgttg cctcgatcag cggcaactcg ctgccgacgt ttgtctgggg
      481 gttgtttctc ctgatggtct tctatggaca gttgggatgg tttcccccgg ggaggctgtc
      541 gctggacgcc gaccagttcg tgcgtacggc ccggttccac gcctacaccg gcctcatgac
      601 cgtggacgcg gcgctgaacc ggcagtggtg ggtcttctgg gacgcggcca agcacctggt
      661 cctgccagtg gcgacgctga cctactacct caccgccgtc ctggtgcgga tcacgcgctc
      721 ctcgatgcta gacaccctgc ggaccgacta cgtgcggacc gcccgggcca aggggctcgc
      781 ggacaaggtc gtcgtcgaca agcacgcgcg ccgcaacgcg ctcatcccgg tcatcacgct
      841 cggcagcctg ctcttcgtcg ggctgctcag cggggtggtg atcacggaga ccgtgttcaa
      901 ctaccccggc atcggccggt ggggcgtcgg ggcgagccag cagctcgaca tcccggcggt
      961 cacggggttt gccctcatct tcgccggcct gctggtcatc gggaacctct gcgcggacgt
     1021 cctgtacgcc ttcgtggacc cgcggatccg gctgcagtga gcgcggtccg gtcctcagcg
     1081 ccggtcatcc cgctcccgcg ggtgccggcc catctaccga aggtcgagac cgggtggcac
     1141 cggacggtcc ggcggttccg ggccaacccg atgtcgatgc tcggcctcgc gatcgtcgcc
     1201 ttctttgtcg cgctggccct cctcgcgccg tttctggcgc acccgaccga gcgcaacccc
     1261 tacctgatcc cccacgaggg gtacgccagc gagccccgtc cgcccagccc ggcccacccg
     1321 tttgggacga ccgaagagca gttcgacctc tactacggcg tgatctgggg ggcgcgcacc
     1381 gccttcaagg tcgcgctgat cgtcgtcgcc tccgcggtcg ccgtcgccct cttcctcggc
     1441 agcctgagcg gcttctacgg cgggtgggtc gacgaggccg tgatgcgggt caccgacatc
     1501 ttcctggcgt ttccggggct gatcctggcc gtggtcatcg tcgccgtgct cgggcagagc
     1561 gtgcagaacg cggtcatcgc gatcgccgtg gtcgaatggc ccacgtacac ccggctcctc
     1621 cgcggggagt tcctccgggt gcgggacatg gagtacgtcc aggcggccag ggcgcttggg
     1681 agcggcgacc tgcggatcgt cgcccgccac ctcatcccca acaccgtgta tcccatcctg
     1741 atcctcgcct cgctcagcat gggcgggatc gtgatcacct tcgccgcgct gtcgttcctc
     1801 gggctcggcg cgccgcccgg gtatgccgac tgggggcagc tcatcagcct gtcccacaac
     1861 tggatcgcgg gcacgcccgg gcatgcgttc acgttctggt atactctggt cgtgcccggc
     1921 gccgccatct tcctgtttgt gctcgggtgg aacctgctcg gggacgcgtt ccgggacatc
     1981 ttcgatccgc gcctgcaggg gagccggtag cagtcgatgg cagcctcgtg cccaagaggc
     2041 gcaactcggg tggataatcc cccagcagcc gcttgattct ccccacagat ggggcagcac
     2101 ttctacatga tcaacaaggg ctgagccgcc gcccgcggtc gagcgggcgg gagcggtccc
     2161 ggcgggacgc tgtgcaggtt cagatagatc cgactacacc tcggtgagtg ccgcgacgga
     2221 aagccgggtt tcggctgcat gtgaagggcc gatatactgg ccccatgagc atcagtggcc
     2281 aagt
//
LOCUS       DASQGH010000427         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13337, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000427 DASQGH010000000
VERSION     DASQGH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13337"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..364
                     /locus_tag="VEW91_08370"
     CDS             194..364
                     /locus_tag="VEW91_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91631.1"
                     /translation="MAATAAETRTTGVTIKGEIVGTFLFVTSPALGFLRAPDGSFTNI
                     DFEALFAVFEGV"
     regulatory      386..522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            617..2950
                     /locus_tag="VEW91_08375"
     CDS             617..2950
                     /locus_tag="VEW91_08375"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HYM91632.1"
                     /translation="MEGQRDFFERMRLILPGRQLIVVSNREPYSHRRGKGGALVVDRA
                     VGGLVAALDPIMQELSGTWIAWGDGEADFEVTDPMGRVRVPPEAPRYALKRIPLTRPE
                     IEGFYYGYANQALWPLCHMAMEYARFRARPWATYQAVNHRFAEAVEAEADPEAVVWLH
                     DYHLALCPRYLRRSRPELFLMQFWHIPWPAWDIFRICPHGADLLDGLLANDLIAFQHP
                     RHVEHFLECAARVLGAQADDNAVEYGGRLIDVKAFPISVDVATLEETARSLRCERWMA
                     RLRRRFNLEGRLVVVSVDRLDYTKGIPERLQAIDLFFRRRPEYRSRVVFIQKTAPSRT
                     RIKAYRDLQERVERDIERLNGVFGTDGWRPVISLPTPLPPVGMAALYRMADLCVVSSL
                     QDGMNLVAKEFVACQIDRRGALALSELTGARDELSWAIPINPYDPERCADALARGLEM
                     PDDERRRRMDHLRAYLGEHDIFRWTLQHVETAAYLIANRPTPGALLEAVEEIRPRVAG
                     ARPVALLADFDGTLVGFADEPDAVVLPDQIRTRLARMARSGRARIGIVSGRALDDIRL
                     RVGIDELVYAGNHGLEIAGPGWTWTHPEAARSCEAVAACCRRVRQRLRGVPGAWVEDK
                     GLTASVHYRGTPHRLVERVHVAVLEDVAQLPSGTLVVHPGKHALEIRPDVAWGKGDAV
                     RWILTSFFGVAWPWEASIVYAGDDRTDEDAFEALADHAVTVKVGPASYPTAARYSLRD
                     IDEVYRFIQLIDAWITVPDATARMEERSRNRRARVTA"
     gene            complement(2860..3594)
                     /locus_tag="VEW91_08380"
     CDS             complement(2860..3594)
                     /locus_tag="VEW91_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91633.1"
                     /translation="MPERFITEREEAAAQPFEIGRIGMHPPDCIGEGLLPLRLEPRVE
                     RGPMAPPGLEEVIVDDRHLRAGKPGAKLRQGPDLEPGGDRDDGRGHEIERLPIDDPRN
                     DRGVRQVVVDDRDQHGPQRLQILEIERIHDREALAQNPVPDLDRRPGGKIVRIGAGQI
                     VMPEEIGVGVVVNALDRHILDMLEELGLIAGDQTEHPLKPVTGDEAKRCRGSGSAHAV
                     TRARRFRERSSMRAVASGTVIQASIS"
     gene            3593..>4024
                     /locus_tag="VEW91_08385"
     CDS             3593..>4024
                     /locus_tag="VEW91_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HYM91634.1"
                     /translation="MATAPRSLDHYRPIAPPGTVDMLYRAAERVRDKRFTHVNSTRLG
                     GGVAEMLHRLLPLLNDLGSVTRWEVIQGDEEFFRVTKAFHNALQGQGQPVTPEMLEHY
                     LETNRRNATRLDLGGDLIFIHDPQPAALIVHRNSGVWIWRCH"
BASE COUNT          631 a         1320 c         1383 g          690 t
ORIGIN      
        1 gactgctgcg acggtacagg ccttaatgcg ggaacatcag gttatcaggg cgccactccg
       61 ggccagatcc aaacggcagg agccgcgatc gcgatcggag gtacagcaat cggcggtccg
      121 gctggcagag cgatgggggt gttaggcggt gcgctcggcc tcctcgggaa ggcactagag
      181 ggcggcggca agggtggcgg caacggcggc ggagacaagg acaacgggag tcaccattaa
      241 aggcgaaatc gtgggaacct tcctgttcgt cacatcaccg gcactgggct ttctgcgtgc
      301 ccccgatggg agcttcacca acattgactt tgaggcgcta tttgccgttt tcgaaggtgt
      361 gtaggattaa atagctcagg catgccctgt gttggtgccg aatccctgcg atccgcaggg
      421 agcgggggat ccaggacgtg gggcgtatct cgcaggattg cgagtaggga ctctcgtccc
      481 gagcccgaca gctaaccccg caggcgacga gagagagggt aggccgggtg gcgccgcgcc
      541 cggagacctt ctccgggcga tttcgtttcc acctgtctcg tcggctcaca gggaagccgc
      601 gggaggtgtg atcccgatgg aaggccagcg cgacttcttt gagcgcatgc ggttgattct
      661 cccaggacgc cagctgatcg tcgtcagcaa tcgagagccg tacagccacc gcagggggaa
      721 aggtggggcg ctcgtggtcg atcgggcggt gggcggcctt gtcgcggcac tcgatccgat
      781 catgcaggag ctctcgggga cgtggatcgc ctggggtgac ggagaagcgg actttgaggt
      841 caccgacccc atgggacggg tcagggtgcc gccggaggct ccccgctacg cgctcaagcg
      901 catcccgttg acccgtccgg agatcgaggg cttctactac gggtacgcca accaggccct
      961 ctggccgctc tgccacatgg ccatggagta cgcgcggttt cgggcccggc cctgggcaac
     1021 gtaccaggcc gtcaaccacc ggtttgccga ggcggtggag gctgaggcgg accctgaggc
     1081 cgtggtctgg ctgcacgatt atcatctggc gctttgcccc cgctaccttc gccggtcgcg
     1141 cccggagctg tttctcatgc agttctggca cattccgtgg ccggcgtggg atatcttccg
     1201 gatctgcccc cacggcgcag acctgcttga cggcctgctg gcaaacgacc tgatcgcctt
     1261 tcagcatccc cgccacgtcg agcacttctt ggagtgcgcc gcgcgcgtgc tgggcgccca
     1321 ggccgacgac aatgccgtcg agtacggtgg ccggttgatc gacgtgaagg cgtttccgat
     1381 cagcgtcgac gtcgcgacgc tggaggagac cgcgcgctcg ctccggtgcg agcgctggat
     1441 ggcgcggctg cggcggcggt ttaaccttga ggggcggctc gtggtggtca gtgtcgaccg
     1501 tctcgattac acgaagggga ttcccgagcg gctacaggcg atcgatctct tcttccgccg
     1561 gcggcccgag taccggtcgc gggtggtgtt catccagaag accgcgccga gccggacccg
     1621 gatcaaggcc taccgcgacc tgcaggagcg ggtcgagcgc gacattgaac gcctcaacgg
     1681 ggtgttcggc acggacggct ggcgcccggt gatctccctc ccgacgccgc tgcctcccgt
     1741 ggggatggcc gcgctgtacc gcatggcgga cctctgcgtg gtcagctcgc tccaggacgg
     1801 catgaacctc gtcgccaagg agttcgtcgc ctgccagatc gaccgccggg gggcgctcgc
     1861 gctgagcgag ctgaccggcg cgcgggatga actctcgtgg gcgattccca tcaaccccta
     1921 cgatcccgag cgctgcgccg acgccttggc gcgcggcctg gagatgcccg atgacgaacg
     1981 gcgccggcgc atggaccacc tcagggcgta cctcggggag cacgatatct ttcggtggac
     2041 gctgcagcac gtcgagacgg cggcgtatct gatcgccaac cgtcccaccc ccggtgcact
     2101 cctcgaggcc gttgaggaga tccgaccgag ggtggcaggc gcgcgcccgg tcgccctgtt
     2161 ggcagacttc gatgggaccc tggtggggtt cgccgacgag ccggatgcgg tggtcctccc
     2221 agaccagatc agaaccaggc tggcacggat ggctcgaagc ggccgggctc ggatcggcat
     2281 cgtgagcggt cgcgcgctcg atgacatccg cctccgcgtc gggattgacg agctcgtgta
     2341 tgcgggtaac cacgggctcg agatcgccgg accggggtgg acgtggacgc acccggaagc
     2401 tgcgcgatcg tgcgaggccg tcgccgcatg ctgccggcgg gtccgccagc gactgcgcgg
     2461 cgttcccgga gcctgggtcg aagacaaggg gctcaccgcc agcgtccact accgcgggac
     2521 tcctcaccgg ctcgttgagc gggtgcacgt cgccgtgctg gaggacgttg ctcagctgcc
     2581 gtccgggacg ctcgtcgtcc accccgggaa gcacgccctg gagatccgcc cggatgtggc
     2641 gtggggcaaa ggcgatgcgg tccggtggat cctcacctcc ttcttcgggg tcgcgtggcc
     2701 ctgggaggcg tccatcgtct acgccggaga cgatcggacc gacgaggacg cctttgaggc
     2761 gctggccgat cacgcggtga cggtcaaggt cgggccggcc tcctacccga cggcggcccg
     2821 gtacagcctc cgcgatatcg acgaggtcta tcggttcatt cagcttatcg acgcctggat
     2881 caccgtgccg gatgcgacgg cgcgcatgga ggagcgctcc cggaaccggc gcgcgcgggt
     2941 gaccgcatga gcagatccgc tgccccggca ccgtttcgct tcgtctcctg tcacgggctt
     3001 cagcggatgc tcggtctgat cgcccgcgat gaggccgagc tcctccagca tatcgaggat
     3061 gtgccgatcg agagcattca ccaccacacc cacgcctatt tcctccggca tcactatctg
     3121 gccggcgcct atccgaacga ttttgccacc tgggcggcga tccaggtccg ggaccgggtt
     3181 ctgggcgagc gcctcgcgat cgtggatccg ttcgatttcg aggatctgga ggcgctgagg
     3241 gccgtgttga tctcgatcat cgacgaccac ctgacgcaca ccccggtcgt tccgcgggtc
     3301 gtctatgggg agccgttcta tttcatgacc acgaccatcg tcgcgatccc caccgggctc
     3361 gaggtccgga ccctgtcgga gtttcgcacc cggcttgccg gcgcggaggt ggcggtcgtc
     3421 tactatcacc tcttcgaggc ccggcggtgc catcggccca cgttcgacgc gtggctcgag
     3481 gcgcaggggc aggaggccct cgccgatgca atccggcggg tgcatcccta tacgaccgat
     3541 ctcgaagggt tgcgcagccg cctcctcgcg ctctgtgatg aagcgctcag gcatggcgac
     3601 tgccccgcga tccctcgatc actaccgccc gatcgcgccg cccggcaccg tggacatgct
     3661 ctaccgcgcc gccgagcggg tccgggacaa gcggtttacc cacgtcaact ccacccgcct
     3721 ggggggaggg gtcgccgaga tgctccatcg tctcctcccc ctgctcaacg atctcgggag
     3781 cgtgacgcgg tgggaggtca tccagggcga tgaggagttc ttccgggtga cgaaggcctt
     3841 tcacaacgcg ctccagggcc aggggcagcc ggtcaccccg gagatgctcg agcactacct
     3901 ggagaccaac cggcggaacg ccacccggct cgatctggga ggcgatctca tcttcatcca
     3961 cgacccccaa cccgcggcgc tcatcgtcca ccggaacagc ggggtgtgga tctggcgctg
     4021 ccac
//
LOCUS       DASQGH010000428         1720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49947, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000428 DASQGH010000000
VERSION     DASQGH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49947"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..>1720)
                     /locus_tag="VEW91_08390"
     CDS             complement(34..>1720)
                     /locus_tag="VEW91_08390"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HYM91635.1"
                     /translation="RDTHPAVLSKSEGRPPAVLSYAELYSRTAAVAAALRQMGIRRGD
                     RVVAYMPNIPETLIAFLAAASLGAIWSSCPPEFGIRSVVDRFRQIEPRVLFAVDGYRY
                     GGGPQDRMDLVAELQRQLPSLEATVLVPYLHEAPPARRLRSARMWPELLATPGDRGLA
                     FDPVPFEHPLWILYSSGTTGLPKAIVQGHGGILLEHLKALSLHLDLRADDRFFWFTTT
                     GWMMWNFLISGLLLGMTVVLYDGSPGYPDLGILWRLAEETGMTYFGASAPYITACQRA
                     GTDPAREASLTKLRGLGSTGAPLPPEGFRWVYEHVKRDLLLGSVSGGTDVCTAFLLSC
                     PLLPVHAGEIQCRGLGAKVEAFDPQGRPVIDQVGELVLTEPLPSMPLHFWNDPDQKSY
                     RASYFETYPGVWRHGDWIKITPRGSCVISGRSDATLNRAGVRMGTSEFYRAVEEIPEV
                     LDSLVVDTGQLGREGRLLLFVVLRSGVELDDALRARIVEKLRRDLSPRHVPNEIYAVG
                     EIPYTLNGKRLEVPVKRILSGVPAEKAVSADAMRNPDSLRFFVDLVGKLNKPT"
BASE COUNT          274 a          598 c          600 g          248 t
ORIGIN      
        1 ctccatccgc gccgtgggga tccgcggccg gcttcacgtg ggcttgttga gcttccccac
       61 caggtcgacg aaaaagcgaa gcgagtccgg attcctcatg gcgtcggcgc tgaccgcctt
      121 ctcggccggc acgcccgaca ggatgcgctt gaccggcacc tcgagccgct tgccgttgag
      181 ggtgtacggg atctccccga cggcgtagat ctcgttgggg acgtggcggg gcgagaggtc
      241 ccggcggagc ttctccacga tgcgcgccct gagggcgtcg tcgagctcca ccccgctgcg
      301 gagcaccaca aagagcagca gccggccctc gcgacccagc tggccggtgt ccaccaccag
      361 gctgtccagc acctcaggga tctcttccac cgcccggtag aactcgctgg tgcccatccg
      421 caccccggcc cggttgaggg tcgcgtccga tcgcccggag atgacgcagc tgccgcgcgg
      481 ggtgatcttg atccagtcgc cgtggcgcca cactccgggg taggtctcga agtagctggc
      541 ccggtagctc ttctggtccg gatcgttcca gaaatgcagg ggcatcgagg gtagcggctc
      601 ggtgaggacc agctcaccga cctggtcgat gaccggccgg ccctgcgggt cgaaggcctc
      661 caccttggcg cccagcccgc ggcactgaat ctcgccggcg tgcacgggca ggagcgggca
      721 cgacaggagg aacgcggtgc agacgtcggt gcctccgctt acggagccca gcagcaggtc
      781 gcgcttgaca tgctcgtaca cccagcgaaa gccctcgggg gggagcggcg cgccggtcga
      841 gccgagcccc cgcaacttcg tcagactggc ctctctcgcc gggtcggtcc cggccctctg
      901 gcaggccgtg atgtagggcg cgctggcgcc gaagtaggtc atgccggtct cctcggccag
      961 ccgccacagg atgcccaggt cgggatagcc cgggctccca tcgtacagca ccaccgtcat
     1021 gccgagcagc agcccgctga tcaggaagtt ccacatcatc cagccggtgg tggtgaacca
     1081 gaagaaccgg tcatccgccc gcagatccag atgcagcgag agcgccttca agtgctccag
     1141 caggatcccg ccgtgcccct gcacgatggc cttcggcagg ccggtggtcc cggacgaata
     1201 gagaatccac aacgggtgct cgaaagggac cgggtcgaag gctaggcccc ggtccccggg
     1261 ggtggccaag agctccggcc acatccgggc gctccgcagg cggcgcgccg ggggtgcctc
     1321 gtgcaggtag ggcaccagca ccgtggcctc gagcgacggg agctggcgtt gcagctcggc
     1381 cacgagatcc atgcggtcct gcggcccacc gccgtaccgg tacccgtcga cggcgaagag
     1441 gacccgcggc tcgatctgcc ggaagcggtc cacgacgctc cggatgccaa actccggcgg
     1501 gcagctcgac cagatcgcgc ccaggctggc cgcggcgagg aacgcgatca gggtctcggg
     1561 aatgttgggc atgtaggcca cgacccggtc gccccggcgg atccccatct gcctgagcgc
     1621 ggcggccacg gcagccgtcc ggctgtagag ctcggcgtag ctgaggacgg cgggcgggcg
     1681 tccttcggac ttggacagca cggcaggatg cgtatcccgg
//
LOCUS       DASQGH010000429         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_49950, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000429 DASQGH010000000
VERSION     DASQGH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49950"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1336)
                     /locus_tag="VEW91_08395"
     CDS             complement(71..1336)
                     /locus_tag="VEW91_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91636.1"
                     /translation="MGSRARAALVTLLCAAAFAMPSGAASAETLADALRTRGLAASPQ
                     VLPNLDKPITSYAVFEDGHEFLIAYYVDDGTGLLHAPLFVNRYDKVARAWTSAEIDGR
                     AAQFSDTWCLGSAVDVRASTHAFYLGTHLNPSAGCTIVLSRDLAVQAVLPGWVLAVFG
                     DGTIVYQRSEVHFAPTHYAELSLYDQTHRRDVQIYPMKPYQRIRTEHINRVRQVYSNE
                     AWCRAHNHHCDPELFDNFLVGEVAISDATRALAFQVAFDNTVYWSDVERWRLEGFRSL
                     RRYMLETGLQGPLSDALFMYLYEDLRSAERFPGRAHMLQTLEADREVLALVAEASLKE
                     RRPDQNWRAFFDALDPRWERPEIWARLATAIAVPPEFTEVAYVYRNVANGLPIEYREM
                     LLTDLRTRFGRFPLQRYLDPDMLRRIFGD"
     gene            1496..1975
                     /locus_tag="VEW91_08400"
     CDS             1496..1975
                     /locus_tag="VEW91_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYM91637.1"
                     /translation="MATRITPPESSARTALWCPTDFDAVAAYTGLRISLGVAMFMHGL
                     ARFIGGIEHFAQPTIAGFARVALPHDAVVFVVYAIPYLEVVIGFPIVLGLFTRWALLA
                     NVALLLVFIFGSSMQQNWGGVGNQLIYTLITSLLLLAVALNRVSLDGLIRRESQPET"
     gene            complement(1990..>2495)
                     /locus_tag="VEW91_08405"
     CDS             complement(1990..>2495)
                     /locus_tag="VEW91_08405"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HYM91638.1"
                     /translation="LEAIALSERPCVFSHSNPRVRANNPRNITDEQIKACAEAGGVIG
                     VCGWGPTLWTRGAQPPSLDDFVGHIEYMVNLVGLDHVGVASDSTTSMRIDHILQHAAE
                     VNVAYPAVTEDYVSKFGNGLDRRYPVSVAALPSVTARLLERGWKRDDVAKVMGGNFLR
                     IWRTVWG"
BASE COUNT          439 a          803 c          789 g          464 t
ORIGIN      
        1 gcctgcttca gcgtctaaga tctcatagcg gatccaccag gttgggacgt catcagcctg
       61 gaagggcggt tcagtctccg aagatgcgtc tcagcatgtc cgggtcgagg taccgctgaa
      121 gcgggaagcg gccgaatcgc gtgcgcaggt cggtgagcag catctcccgg tactcgatcg
      181 gcagaccgtt ggcgacgttc cggtagacgt aggcgacctc cgtgaactcc ggggggacgg
      241 cgatcgccgt tgccagccgc gcccagatct ccgggcgctc ccaccgcggg tccagcgcat
      301 caaagaacgc tcgccagttc tggtccggcc gccgctcttt gagggacgcc tccgccacga
      361 gggcgagcac ctcgcgatcc gcttccaacg tctgcagcat atgggccctg cccgggaatc
      421 gctcggcgct ccggagatcc tcgtagagat acatgaagag cgcatcggag agcgggccct
      481 ggagcccggt ctcgagcatg tatcgccgga ggctgcggaa gccctccagc ctccagcgct
      541 cgacgtcgct ccagtagacc gtattgtcga acgcgacctg gaacgcgagc gcgcgtgtgg
      601 cgtcgctgat cgcgacctcc ccgacgagga agttgtcgaa caactcgggg tcgcaatggt
      661 ggttatgcgc ccgacaccat gcctcgttgc tgtagacctg ccgcactcgg ttgatgtgct
      721 cggtccggat acgttgatag ggcttcatgg gatagatctg cacatctcgg cgatgcgtct
      781 gatcgtacag ggaaagctct gcgtagtggg taggggcaaa gtggacctca ctgcgctggt
      841 acacgatcgt cccgtcgcca aacacggcca agacccagcc cggaaggacc gcctggacgg
      901 ccagatctcg ggagagcacg atcgtgcagc ccgccgatgg gttgaggtgt gtcccgaggt
      961 agaaagcgtg ggtggaggcc cggacgtcca ccgccgaccc caggcaccag gtatcagaga
     1021 actgagcggc ccggccgtcg atctcagcgc tcgtccacgc ccgcgcgacc ttgtcatagc
     1081 ggttgacgaa gagcggtgcg tgcagcaagc cggtgccgtc gtccacataa taggcgataa
     1141 ggaattcatg cccgtcttcg aagacggcgt aactggtgat gggcttgtca aggttaggga
     1201 ggacctgggg gctggccgcc agaccgcgag ttcggagcgc gtccgcgagg gtttcggcgg
     1261 aggctgcgcc cgacggcatc gcaaatgccg ccgcacatag caacgtgacc agcgccgcac
     1321 gggcccgtga ccccacgggt gccctccctc tgaagtgggc cttgaggtgg gcttgctcac
     1381 ctgagaagat gcccaaaatc cccttcgtcc accccgtcgg aggacgatcc ggtcgctccg
     1441 gcgaacgtcg gcttttaaag ggagcgtgga cgttccggct ggaaaggaga tgcgaatggc
     1501 cacccggatc acgccgcccg aatcgtcagc tcggacggca ctgtggtgcc ccacggactt
     1561 cgacgccgtg gcggcgtaca caggattgcg catttctctt ggggttgcca tgttcatgca
     1621 tggcctcgcg cggttcattg gaggcatcga gcatttcgcg caacccacga tcgcgggctt
     1681 tgcgagagtg gcgctcccgc acgacgccgt cgtcttcgtc gtctacgcga tcccgtatct
     1741 cgaggtggtc attggctttc cgatcgttct cgggctgttc acgcgctggg ctctgctcgc
     1801 gaacgtcgcg ctgctcctcg tctttatttt cgggtcgagt atgcagcaga actggggtgg
     1861 cgtcggcaat caactgatat atacgctcat cacctccctg ttgctgctcg ccgtagccct
     1921 caaccgtgtg tcgctggacg gcctcatacg acgggaaagc caacctgaga cctagcgcgt
     1981 ctcgccaccc tatccccaaa cggtacgcca gatgcgcagg aagtttccgc ccattacctt
     2041 cgcgacatca tcgcgcttcc atccgcgctc aagcagcctc gccgtcacac tggggagcgc
     2101 cgcgaccgaa acggggtagc gccggtcgag accgttcccg aacttcgaca catagtcttc
     2161 cgtcaccgcg ggataggcga cgttgacctc ggcggcatgc tgcaggatgt ggtcaatgcg
     2221 catgctcgtg gtggaatcgc tggccacccc gacatgatcc aaccccacca ggttcaccat
     2281 gtactcgatg tgccccacga agtcgtcgag gctcgggggt tgcgcgccac gtgtccacag
     2341 ggtcggaccc cacccacaca ctccgatcac tccgccagct tccgcgcacg ccttgatctg
     2401 ctcatcggtg atgttccggg ggttgttcgc tctcacccgg ggattgctgt gggagaacac
     2461 gcacggtctc tcgctcaggg cgatcgcctc gaggc
//
LOCUS       DASQGH010000430         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33100, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000430 DASQGH010000000
VERSION     DASQGH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33100"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VEW91_08410"
     CDS             complement(<1..885)
                     /locus_tag="VEW91_08410"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYM91639.1"
                     /translation="MSADLWAITPELVLVALGVALLVVELVAGPGQKRVVGWVGVAGL
                     VVALIPSVDLLFGAPRFAFSETYAIDPFAAFFKVIVITSGALVLLAAMEFFRSHRTHH
                     EGEIYGLVVFMVLGLVLMSAAVDLILLFLALEFVSLISYVLAGSLKSDRKSNEAGVKY
                     FFYGATASAVMLYGFTFLYGISGTTSLYRLMQHVAFFTPGFLGLTVMLILAGLGFKIS
                     LVPFHQWTPDVYEGAPTPITAFLSVGSKAAAFAALLRVLYVALPSQGWVTILAGLAAA
                     SMTLGNLVALSQQNIKRML"
     gene            complement(882..>2280)
                     /locus_tag="VEW91_08415"
     CDS             complement(882..>2280)
                     /locus_tag="VEW91_08415"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYM91640.1"
                     /translation="GFGIAMLAKFHTRHDAANPLQFLVDKKWIDVINSRYIVGLDGIS
                     MPLFLLTLLIVPLCIVYSWNHFPEPHNPKAFLILILILETGMIGAFAATDLFAFYVFW
                     EVMLVPMYFIIGIWGGPRRTYAAIKFFLYTLIGSLAMLLAILIVYFHAAPRTFDILAL
                     IRQQPLSHNLPLAVLAFWGFFLSFAIKVPMFPFHTWLPDAHVEAPTAGSVLLAAVLLK
                     LGTYGFVRIGLPLLPQAFAHLAGIVAVLAVIGAVYGALVAMAQTDLKKLVAYSSVNHM
                     GYVMLGVAAAAAAAGRPDLAGAATTALNGATIQMLAHGVITGALFFLVGVIYDYRAHT
                     RGVDDFGGLGARLPVYTGVTMLAMLASLGLPGLMGFVAEFLIFLGAFQIYPVFTVLAL
                     VGVVVTVAYFLWAIHRIFFGPLNARWAALPDLDARERWALVPLAAIMVLLGVYPRPLV
                     DAVNLAMGAILGMSR"
BASE COUNT          483 a          724 c          783 g          290 t
ORIGIN      
        1 gagcatgcgc ttgatgttct gctgggagag ggccacgagg ttgcccaggg tcatcgaggc
       61 ggccgccagc cccgcgagga tcgtgaccca tccctgcgaa ggcagggcca cgtacagcac
      121 gcggagcagg gcggcgaacg ctgccgcctt gctcccgacc gacaggaacg cggtgatcgg
      181 cgtgggtgcg ccctcgtaga catcgggagt ccactggtgg aatggcacga gcgagatctt
      241 gaaccccaat ccggccagga tcaacatcac ggtcagcccg aggaacccgg gcgtgaagaa
      301 ggcgacgtgc tgcatcaggc gatagaggct ggtggtcccg gagatcccgt acagaaatgt
      361 gaacccgtac agcatcaccg ccgaggccgt cgcgccgtag aagaaatact tgaccccggc
      421 ctcgttgctc ttccgatcgc tcttgagcga accggcgagc acgtaggaga tgagggagac
      481 gaactcgagc gccaggaaga gcaggatgag gtccacggcg gcggacatga ggaccaaccc
      541 gagcaccatg aaaaccacca ggccgtagat ctcgccctcg tggtgcgttc ggtggctccg
      601 aaagaactcc atcgcggcga gcagcacgag ggcgccgctc gtgatcacga tcaccttaaa
      661 gaacgccgcg aacggatcga tcgcgtacgt ctcggaaaac gcgaaccggg gcgcgccgaa
      721 cagcaggtcc accgagggga tgagcgcgac gacaagaccg gccacgccga cccagccgac
      781 cacccgcttc tgcccgggcc ccgccaccag ctcgaccacg agcagggcga cgccgagggc
      841 aacgagcacc agttcggggg tgatggccca caggtcggcg ctcaccggct catccccagg
      901 atcgccccca tcgccagatt gaccgcgtcg acgagcggcc ggggatacac ccccaggagc
      961 accatgatcg cggcgagcgg gacgagggcc caccgctcgc gggcgtccag atcgggcagc
     1021 gccgcccagc gcgcgttgag cgggccgaag aagatccggt ggatcgccca gaggaaatac
     1081 gccacggtca cgaccacgcc gaccagggcc agcacggtga agaccgggta gatctggaac
     1141 gcccccagga agatcaagaa ctccgcgacg aaccccatga gccccggcag gccgagcgag
     1201 gcgagcatgg ccagcatcgt cacgcccgta tagacgggca gccgagcccc cagcccgccg
     1261 aagtcatcga ccccgcgcgt atgggcgcgg taatcgtaga tgacgccgac gaggaagaac
     1321 agcgcccccg tgatcacccc gtgggccagc atctgaatgg tcgccccgtt gagcgccgtc
     1381 gtggctgcgc cggccaggtc ggggcggccc gcggccgcgg cggccgcggc gacaccgagc
     1441 atgacgtagc ccatgtggtt gacgctggag tacgccacga gcttcttgag atcggtctgg
     1501 gccatggcca ccagggcgcc atagactgcg ccgatgaccg cgaggacggc gacgatgccg
     1561 gcgaggtggg caaacgcctg cgggagcagc ggcagcccga tccggacgaa tccgtaggtc
     1621 ccgagcttca gcagcaccgc ggcgagcagg acgcttccgg ccgtgggcgc ctcgacgtgg
     1681 gcatcgggca gccaggtgtg aaacggaaac atcggcacct tgatcgcgaa gctgaggaag
     1741 aacccccaga acgccaggac cgccagcggc aggttgtggc tcagcggctg ctgccggatc
     1801 agcgcgagga tgtcgaaggt ccggggcgcg gcatggaagt acacgatcag gatcgccagc
     1861 agcatcgcca gcgatccgat cagcgtgtag aggaagaact tgatcgccgc gtaggttctc
     1921 cggggcccgc cccagatgcc gatgatgaag tacatcggca ccagcatcac ctcccagaag
     1981 acgtagaagg cgaagaggtc cgtcgccgcg aaggcgccga tcatcccggt ctcgaggatg
     2041 aggatcagga tgaggaaggc tttcggattg tgcggctcgg ggaagtggtt ccagctgtac
     2101 acgatgcaca gcggaacgat aagcagcgtc aacaggaaga gcggcatcga tatgccgtcg
     2161 aggccgacga tgtagcggct gttgatgacg tcgatccact tcttgtcgac caggaactgc
     2221 aacgggttcg cggcatcgtg gcgagtgtgg aacttggcga gcatcgcgat gccgaagccg
//
LOCUS       DASQGH010000431         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55568, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000431 DASQGH010000000
VERSION     DASQGH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55568"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..745
                     /locus_tag="VEW91_08420"
     CDS             125..745
                     /locus_tag="VEW91_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91641.1"
                     /translation="MSTRLDYHDPFHEFAGRLTEALEDHHLVVSGYAFLVDVDHMSRE
                     YRYVVHLDNEPQADAPRTFAWPWAELRFLVDALSFGRHIAEYDEGVEFSRTVHEEVQV
                     FGELVTEIMMDRLLVPPDVVALRRAIVPVSAEPGIPSISLQQHFSSEDAPPVYSARVQ
                     SSVMLKEDELVDGDRPRKIASAVSGILNALQALHLPAEGAPLPPAE"
     gene            759..1250
                     /gene="tadA"
                     /locus_tag="VEW91_08425"
     CDS             759..1250
                     /gene="tadA"
                     /locus_tag="VEW91_08425"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HYM91642.1"
                     /translation="MGEAADAAFMGEALAEAEAAARLGEVPIGAVVVRDGRVIARGHN
                     RRETDRDPTAHAEAEVLRQAAGVLGGWRLLGCTLYVTLEPCVMCAGAMVQARLPRLVY
                     GTADPKAGAAGSVVDVLRDARFNHMVEVASGVREAECGALLQEFFARLRRGASGGDIP
                     AWP"
     gene            complement(1665..>2466)
                     /locus_tag="VEW91_08430"
     CDS             complement(1665..>2466)
                     /locus_tag="VEW91_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HYM91643.1"
                     /translation="TWWQTENGGFLCSTKPALDPMKPGSTGPGVPGIFPVIYDEEGNE
                     IPAGSHRAGNICIRNPWPGIMQTIWGDPDRYVETYYAKYCTDPKSKDWRHWPYLSGDG
                     ATLAADGYFRILGRVDDVINVAGHRLGTKELESACLTVPEVAEAAVIPVVDELRGRVP
                     EVYVSLKPGIPPSKEIEEKVVRTIETTIGKIARPKDVRIVPDMPKTRSGKIMRRVLTA
                     ISNAMDTGDVSTLANPDIVEQIRQMVQGAGPVATKAGPEDLKRFGEEP"
BASE COUNT          353 a          846 c          836 g          431 t
ORIGIN      
        1 caccgtgttc ctggacagcc tcccccggct gctgctgcga tcctgccagg gccaggtgca
       61 gaagatgcgc gaggcggcgg cgcctgcggc cagcgggacg acgccgccgg gggtccggta
      121 acgcgtgtcc acgcgcctcg actatcacga tcccttccac gagtttgcag ggcggctgac
      181 cgaggcgctt gaggatcatc acctggtcgt gtcgggatac gcgttcctgg tggatgtgga
      241 ccacatgagc cgggagtacc gctacgttgt ccacctcgac aacgagccgc aggcggatgc
      301 gccgcggacg tttgcgtggc cctgggcgga gctccgcttc ctcgtcgacg cgctttcctt
      361 cggtcggcac atcgccgagt acgatgaggg ggtggagttc tcccggaccg tgcacgagga
      421 ggtccaggtc ttcggcgagc tggtcacgga gatcatgatg gaccggctcc tcgtgcctcc
      481 cgacgttgtg gcgctccgcc gcgccatcgt tccggtgtcc gcggaacccg ggattcccag
      541 catcagcctc cagcagcatt tctcaagcga ggatgcgccc ccggtctaca gcgcccgcgt
      601 ccagtcgagc gtcatgctca aggaggacga actggtcgac ggcgaccggc cccgcaagat
      661 cgcctcggcc gtctcgggga tcctcaacgc cctgcaggcc ctccacctcc ccgccgaagg
      721 ggcgcccctt ccccctgcgg agtaggcacc cccgggccgt gggcgaagcc gccgacgccg
      781 cgttcatggg cgaagcgctc gccgaggcag aagccgccgc ccgcctcggg gaggtgccga
      841 tcggcgcggt cgtggtacgg gacggccgtg tgatcgcccg cggtcacaac cgccgggaga
      901 ccgaccgcga tcccaccgcg cacgcggagg cggaggtcct gcggcaggcg gcgggcgtgc
      961 tgggcgggtg gcggctcctt gggtgcacgc tgtatgtcac cctcgagccg tgcgtgatgt
     1021 gcgcgggcgc gatggtgcag gcccgcctgc cccggctggt ctacggcacg gccgatccca
     1081 aggcgggcgc cgccggcagc gtggtcgatg tgctccggga cgcccggttc aaccacatgg
     1141 tcgaggtcgc ctcgggcgtg cgcgaggccg agtgcggcgc gcttttgcaa gagttcttcg
     1201 ccaggctgcg gcgtggagcg agcggggggg atatccctgc gtggccgtga cgctcggcgg
     1261 gctagactcg cagggctgct ggggctagag cgccgccttc ctcacggccg caccgcgtgc
     1321 gtgcagtagt cctcggggtg gcgcatagac tcacagttac ccctgcggga gaacggcccc
     1381 atggccgttt gcgtttgcgg gttcaccaca taccaacctg tcgcgggcca ctctccggca
     1441 ttgaccggca cgcacatgct gtactgaggc agatgggcca cagtctcctc gcacgtcgcc
     1501 gacgaactaa tggggccatt gtagagcccc aggctcggcg tgtagaccca ccacaccaca
     1561 accaaggctg ataccagaga tgaaacgcgc ctcattctcc tggacccaag cggttcgaca
     1621 tgactcctca cggctatatg cccaatgcgc ccatggttca gcgatcatgg ttcctcgccg
     1681 aaccgtttga ggtcctcggg gccggctttg gtcgcgaccg ggcccgcgcc ctgcaccatc
     1741 tgccggatct gctcgacgat atccgggttg gccagcgtgg aaacgtcccc cgtgtccatc
     1801 gcgttggaga tcgcggtcag gacccggcgc atgatcttcc cggagcgggt cttgggcatg
     1861 tccgggacga tccgcacatc cttcggccgc gcgatcttgc cgatggtcgt ctcgatcgtt
     1921 cggacgacct tctcctcgat ttccttgctg gggggaatcc ccggtttcag cgagacgtac
     1981 acctcgggga cgcggcccct gagttcgtcc accacgggaa tcaccgcggc ctcggcgact
     2041 tccggcaccg tcaggcacgc cgattccagc tccttggtgc ccaggcggtg tccggccacg
     2101 ttgatgacgt catcgacccg gcccaggatg cggaagtatc catcggccgc cagcgtcgcg
     2161 ccgtcgcccg agaggtacgg ccagtggcgc cagtccttgc tcttcgggtc cgtgcagtat
     2221 ttcgcgtagt acgtctcgac gtagcgatcg gggtcccccc agatggtctg catgatcccg
     2281 ggccaggggt tgcggatgca gatgttcccg gccctgtggg acccggcggg gatctcgttg
     2341 ccttcctcgt cgtagatcac ggggaagatc ccgggcaccc cggggccggt gctccccggc
     2401 ttcatggggt ccagcgccgg cttggtgctg cagaggaacc ccccgttttc ggtctgccac
     2461 caggta
//
LOCUS       DASQGH010000432         7479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18906, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000432 DASQGH010000000
VERSION     DASQGH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18906"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VEW91_08435"
     CDS             complement(<1..299)
                     /locus_tag="VEW91_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91644.1"
                     /translation="MSRVLLVLLLVTTLSTPGLAASSALLPYNLVLSPRPASYASNGV
                     IAGQFGGVPVAGAYRGSGSTGTLTLTVHEATFMDATYTCSAAGCTVTGTVAGNRVT"
     gene            complement(392..1222)
                     /locus_tag="VEW91_08440"
     CDS             complement(392..1222)
                     /locus_tag="VEW91_08440"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM91645.1"
                     /translation="MFSLSGKTCVVTGASRGIGRAIALAFAEQGADVVLAARTRDDLE
                     KVRHLIEAQGRRAAIQPTDVTDLDQLRKAAEAAVTHFGKIDVWVNNAGGFTAAAGSTS
                     EWLDVTPAGWEQMLRLNLTAQVFGAQAAARVMRNQGTGGVILFLSSIDSLYAAPGGEG
                     IYGACKAALNNITQTMAVELGQHRIRVNAIAPAVVETPLTAPWLATEEDRQSRAAFYP
                     LRRIGQPADVAAAAVYLASDEAAWVSGAVLLVSGGAVMTSDPYRYLMRVNQAGRREAL
                     "
     gene            complement(1238..1843)
                     /locus_tag="VEW91_08445"
     CDS             complement(1238..1843)
                     /locus_tag="VEW91_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003555093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HYM91646.1"
                     /translation="MIESRTLVTIPARRGKAARVRNGQVITIINTHGQQVVDTWAFNA
                     RDITEFMSMEHSRAAIAKIIPEVGDILVTNHRRPVLQVGEDTTPGVHDTLIAACDRYR
                     YELLGCKEYHDNCADNLAAALKALGEVAPETPSPLNLFMNIPVSDGRYVAFRPPVCGP
                     GQHIALRAQMDLIIAFSACPQDIIPINGAACTPTEAHFTVA"
     gene            complement(1840..2937)
                     /locus_tag="VEW91_08450"
     CDS             complement(1840..2937)
                     /locus_tag="VEW91_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HYM91647.1"
                     /translation="MSLPRDRPTPRQKPVVAVLARPDGPPPPGLGPVAKVATIRHAAS
                     AQALAEALEDAEVLLVLDFRSTTLRDAWPRARHLRWIHVAAAGVDTVLFPELVSSSVT
                     LTNSRGVFDQAIAEYVLGLILAFAKDFPATLDLQRRRVWQHRETEHVRGQTVLVVGAG
                     GLGRAIGRLAGSAQMRVVGVARTARASDPDLGRVVAARDLSAALGEADYVVVAAPLTS
                     ETRGLIGPAAFGRMKPTARLINVGRGPIVDEAALLDALQTKRIAGAALDVFMQEPLPK
                     DHPFWDLPGLIVSPHMSGDFVGWMSALSELFVENYARWRRGAPLLNVVDKTLGYVPST
                     HRPQAGVKDVPRRAAKSEMLGATPKHRRRLP"
     gene            complement(2942..3883)
                     /gene="speB"
                     /locus_tag="VEW91_08455"
     CDS             complement(2942..3883)
                     /gene="speB"
                     /locus_tag="VEW91_08455"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYM91648.1"
                     /translation="MTHPRPVDSMVYPRYSGIATFMRLPHIPDARGLDVAFVGVPFDT
                     SAGYRGGARLAPRAIRHHSSQVRLHHPIHDVTIHEEIRIADYGDVRTSPFEIEKTFDW
                     ITEEFDKIHAQGCKTIAVGGDHGITYPILRAVARHHGPVGVVQIDAHTDTHDLQFGYK
                     LTHATPFRRAHEAGLITPEKVMQLGIRGSLFYPDDLTWSKEHYRMVPVHEIHRRGMDD
                     VVVEMRALAGTVPVYLSFDIDGLDPSCAPGTGVPEIGGLSTWQAIQVIHGFAGLNLVG
                     ADVVEVSPPCDTNDITSIVAAQLLFEIAAVFAVNKAR"
     gene            complement(3950..4915)
                     /locus_tag="VEW91_08460"
     CDS             complement(3950..4915)
                     /locus_tag="VEW91_08460"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYM91649.1"
                     /translation="MTYLEAIADGLRQAMRADPSVILLGEDVGAYGGAFKLTKGFLEE
                     FGPRRVIDTPISEAAIVGAAVGAALVGLRPVAEMQFADFISNAFNQIVNVAATSAYRA
                     GGCVPLVIRAPCGAGTHGGPFHSQSVEAYFFHTPGIKIVLPATPADAKGLLLSAIADP
                     NPVLYLEHKALYRSQRGPVDEGLTFVPLGKAAVRRPGRDVSVIAYGMMVHRCLEAAEA
                     AAQEGVDAEVIDLRTLLPLDLETLTASVTRTSRVLIVHEDRLRGGVGAEIAAYIGEHL
                     FASLDGPIGRVGAPDIPVPYAPPLEAAYLPSVERIRRAIVTRARW"
     gene            complement(4915..5940)
                     /locus_tag="VEW91_08465"
     CDS             complement(4915..5940)
                     /locus_tag="VEW91_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYM91650.1"
                     /translation="MEAGRGDITAAPLAAVPPGLAADDLRTLFRHMLLQRLAEDRIVK
                     LYYQGAVEGACFTGYGHEAIAVGAASALGPQDVVSTLHRDLGARLVLGMPVRYYFANF
                     LGRTGSPTRGREGNLHIGGLGPRILLHIDHIGASIPVAAGAALGFTVRQEARVAMAFF
                     GEGTLATGEFHEGANLAAVLRLPLVLVCWNYQFAYSTPVSREVAGPLVDRMAGYGMPA
                     CSVDGNDILAVYDAARRAVDRAREGQGPSFLECRTMRIRGHSEADDASYVPKALIEEW
                     RRRDPVERFEKHLLETQVLTARQVEALRAAVLAEVDEAQAWAEASPPPDPSELERGVY
                     CEGRPWP"
     gene            complement(5927..6688)
                     /locus_tag="VEW91_08470"
     CDS             complement(5927..6688)
                     /locus_tag="VEW91_08470"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYM91651.1"
                     /translation="MRLRDRVAVITGSSMGIGEAIAWTYAREGARIVINSRARERGER
                     VAGALRGEGHDAIAVAGDVREKASADRLVAAARQRWNRLDILVNNAGTSMIGPSEALP
                     EEGWRLTIDTNLTGAFFCAQAAAQVMIPQRKGVILNISSILGEVGLPKRAAYCASKHG
                     LIGLTKVLAAEWARHGIRVVSIDPAYIKTAMDDRDQVSGDYTNADIERRTPAGRFGTT
                     EEVARVAAFLASDDAGYITGSCVTVDGGWVAYGGW"
     gene            complement(6720..>7479)
                     /locus_tag="VEW91_08475"
     CDS             complement(6720..>7479)
                     /locus_tag="VEW91_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HYM91652.1"
                     /translation="MTGEDVGTTLREMEYMRMETPYVVTLPASWGGAGPIGGATAFGV
                     VQGMKACAKAVYDSDSLEGRTVTVQGLGVVGSEVVPLLVKEKARVVVTDIDREKVEAI
                     TSRFSLPAVPPDEIIGRVADIFCPCALGGVINAEAIPRFKVKIICGSANNQLREERDG
                     QEIDARGILYAPDYIVNAGGTIFDTDRLNPGGFNRERAMTNVSRIYETMASLIRISRQ
                     EKIPTYRAADLLAERRIEAVRKAKLLAAHGEVRM"
BASE COUNT         1180 a         2586 c         2528 g         1185 t
ORIGIN      
        1 gtcacacggt tccccgccac cgtccccgtc actgtgcagc ccgcggcgga gcaggtgtag
       61 gtcgcgtcca tgaatgtcgc ctcgtgcacg gtcagggtca atgtccccgt tgatccgctg
      121 ccgcggtagg ccccggcgac cggcacccca ccaaactggc cggcgatcac gccattggag
      181 gcatacgacg ctggcctcgg agataacacg aggttgtacg gcaaaagcgc gctagaggcg
      241 gccagtccgg gagtactcag cgtcgtcacc agaaggagta ccagcagcac tcggctcact
      301 cgggccctcc ttggttcggc ggaaatccgc gctcccggga catcgtacgg ccggtacttc
      361 acagtcctgt gtgttcccgg tcacacccgc gtcacagcgc ttctctgcga ccggcctggt
      421 tcacccgcat gaggtatcgg tagggatcgc tggtcatgac cgctcccccc gagacgagga
      481 ggacggctcc cgacacccag gccgcttcat cggaggccag gtagaccgcg gcggcggcga
      541 cgtccgcggg ctgcccgatg cgccgcagag gatagaacgc cgcgcgggac tgccgatcct
      601 cctccgtggc aagccacggc gcggtgagtg gcgtctcgac gacggcgggc gcaatcgcgt
      661 ttacgcggat ccggtgctgc ccgagctcga ccgccatcgt ctgcgtgatg ttgttgaggg
      721 cggccttgca ggcgccgtag atgccctcgc cccccggcgc ggcgtacagg ctgtcgatgg
      781 aggagaggaa gagaatgacc ccacccgtgc cctggttccg catcacacgc gcggcggcct
      841 gcgccccaaa cacctgggcg gtcaggttca accggagcat ctgctcccag ccggccgggg
      901 tcacgtcgag ccactcgctt gtgctgcccg ccgccgcggt gaacccgccg gcattgttga
      961 cccagacatc gatcttcccg aagtgcgtga cggcggcctc ggccgctttc cgcagctgat
     1021 cgaggtcggt gacatccgtc ggctggatgg ccgcacgccg cccctgggct tcgatcagat
     1081 gccgcacctt ctccagatcg tcgcgggtcc gggcggccag caccacgtcg gccccctgct
     1141 ccgcgaaggc aagcgcgatc gcgcggccga tcccgcggct cgcccctgtc accacgcagg
     1201 tttttccgga gagtgagaac atacgccgtg accgaggtta ggcgacggta aagtgcgcct
     1261 cggtcggcgt gcaggcagcg ccgttgatcg ggatgatatc ctgcgggcac gcggagaacg
     1321 cgatgatgag atccatctgc gcccggagcg caatgtgctg gcccggtcca cacaccggag
     1381 gccggaatgc cacgtaccgg ccgtcggaca ccgggatatt catgaagaga ttcagcggcg
     1441 acggggtctc cggagccacc tccccgagtg ccttcaaggc ggccgcgaga ttgtccgcgc
     1501 agttgtcgtg atattccttg cagccgagca gttcgtagcg ataccgatcg caggcggcga
     1561 tgagcgtatc gtgcacgccg ggggtcgtgt cttcacccac ttggagaacg ggtcttcggt
     1621 ggtttgtgac gaggatatcg ccgacttcag ggatgatctt ggcgatggcg gcccggctgt
     1681 gctccatcga catgaattcc gtgatatctc gggcattgaa ggcccaggtg tccacgacct
     1741 gctggccgtg ggtattgatg atcgtgatca cctggccgtt ccgaacgcgt gcggccttgc
     1801 ctcgccgggc gggaatcgtg acgagcgttc gggattcgat cacgggagac gcctcctgtg
     1861 cttcggggtc gcgccgagca tctcgctttt cgcggcgcga cgcggaacat ccttgacgcc
     1921 cgcctgcggc cggtgggtcg acggcacgta gcccagcgtc ttgtcgacca cattgagcaa
     1981 cggcgctccc cgccgccagc gggcgtagtt ctcgacgaac agctccgaca gagccgacat
     2041 ccatccgacg aagtcgccgg acatgtgggg cgagacgatc agccccggca gatcccagaa
     2101 gggatggtcc ttcgggagcg gctcctgcat gaacacgtcc agcgcggccc cggcgatccg
     2161 cttcgtctgc agcgcgtcga ggagcgcggc ttcgtccacg atcggtcccc ggcccacatt
     2221 gatcaggcga gccgtcggct tcatccggcc aaacgcggcg gggccgatga ggcccctggt
     2281 ctcggacgtc aggggggcgg cgacgacgac ataatcggct tcccccaggg cggccgagag
     2341 gtcgcgggcg gccaccacgc gtcccagatc aggatcggag gcgcgggcgg tccgagccac
     2401 ccccacgacc cgcatctggg cggagccggc cagcctcccg atcgcccgcc cgagcccgcc
     2461 cgcgcccacg acaagcaccg tctgtccgcg cacatgctcg gtctcccggt gctgccacac
     2521 acggcgccgc tggagatcca gtgtcgccgg aaaatctttg gcaaacgcca ggatgagccc
     2581 gagcacgtac tcggcgatcg cctgatcaaa cacccctcgg gaattcgtca gggtcacgct
     2641 gctcgacacc agctcgggga acagcaccgt gtcgacgccg gcggcggcca cgtggatcca
     2701 gcggaggtgc ctggcccggg gccaggcgtc gcgcagcgtg gtggagcgaa agtccaggac
     2761 caggaggacc tccgcgtcct cgagggcttc cgcgagcgcc tgggccgacg ccgcgtggcg
     2821 gatcgtcgcc accttcgcca cgggcccgag ccccggaggc ggcggcccat ctgggcgggc
     2881 gagcacagcc accacaggct tctgccgcgg ggtgggacga tcgcgcggga gggacatgcg
     2941 gtcagcgcgc cttgttgacg gcgaacaccg cggcgatctc gaacaacagc tgcgccgcga
     3001 caatcgaggt gatgtcgttg gtgtcgcagg gaggggagac ttccaccacg tccgcgccca
     3061 caaggttgag ccccgcgaag ccgtgaatga cctggatcgc ctgccacgtg ctcagcccgc
     3121 cgatctcggg gacgccggtc cccggagcgc acgacgggtc caggccgtcg atgtcgaacg
     3181 acaggtagac cggaacagtc cccgcgagcg cgcgcatctc caccacgaca tcgtccatcc
     3241 ctcgcctgtg gatctcgtgc acaggcacca tgcgatagtg ctccttggac cacgtgagat
     3301 cgtccggata gaacaggctg ccgcggatgc cgagctgcat caccttctcc ggagtgatca
     3361 gcccggcttc gtgcgcccgg cggaacgggg tcgcgtgggt cagtttgtac ccgaactgga
     3421 ggtcgtgggt gtcggtgtgg gcgtcgatct ggacgacccc gaccggcccg tggtgtctgg
     3481 cgaccgcgcg caggatcggg tacgtgatgc cgtggtcacc gcccacggcg atcgtcttgc
     3541 acccctgagc atggatctta tcaaattcct ccgtgatcca gtcaaacgtt ttttcgatct
     3601 cgaagggcga cgtgcgcaca tcaccgtagt cggcgatccg gatctcttcg tggatggtga
     3661 cgtcgtggat cggatgatgc aggcgcacct gactggagtg gtgtcggatc gcgcggggcg
     3721 ccagccgggc gcccccgcgg tagccggcgc tcgtatcgaa tgggaccccg acgaaggcga
     3781 cgtccaggcc gcgcgcatcg gggatgtgcg ggaggcgcat gaacgtcgcg atgcccgaat
     3841 agcgggggta taccatgcta tcgacgggcc gaggatgcgt catggcgcgt gctcctttct
     3901 cccccttcgt cgaccggcgg cgtgggagag gtcgcttgcc caccgccggc taccagcggg
     3961 cgcgggtgac gatggcgcgg cggatgcgtt cgacgctggg gaggtacgct gcttcgagcg
     4021 gaggcgcata ggggaccggg atgtcgggcg cgccgacgcg gccgatcggg ccgtcaagcg
     4081 acgcaaagag gtgctccccg atgtacgcgg cgatctcggc tccgacgccc ccccggagcc
     4141 gatcctcgtg gacgatgagc acccggcttg tgcgcgtcac cgatgccgtg agggtctcga
     4201 ggtcgagcgg aagcagcgtg cggagatcga tcacctcggc gtccacgccc tcctgcgcag
     4261 ccgcctcggc cgcctcgagg cagcgatgga ccatcatccc atacgcgatc acgctcacgt
     4321 cgcgccccgg gcgccgcacc gccgccttgc ccagagggac gaacgtcagg ccctcgtcga
     4381 ccggaccgcg ctgggaccga tacagggcct tgtgctccag atagaggacc gggttgggat
     4441 ccgcgatcgc cgagagcaac aaccccttcg cgtccgcggg ggttgcgggg agcacgatct
     4501 tgatcccggg ggtgtggaaa aaatacgcct ccaccgattg cgagtgaaac ggcccgccgt
     4561 gggtgcctgc gccgcagggc gcgcgaatga caaggggcac acagccgccg gcgcggtagg
     4621 cggacgttgc ggcgacgttg acgatctggt tgaacgcgtt ggagatgaag tcggcgaact
     4681 gcatctcggc gacagggcgc agaccgacca gggccgctcc gaccgccgcg cccacgattg
     4741 cggcctcgct gatgggcgtg tcgatgacgc ggcgggggcc aaactcctcc aggaaccctt
     4801 tcgtgagctt gaacgcccct ccgtaggccc ccacgtcctc cccaaggagg atgacggacg
     4861 gatccgcgcg catcgcctgc cgcaggccgt cggcgatggc ctccaggtag gtcatcacgg
     4921 ccacggccgg ccctcacaat agaccccgcg ctccaactcg gagggatccg ggggcgggct
     4981 ggcctccgcc cacgcctggg cctcgtccac ctcggccagg acggccgccc gaagggcctc
     5041 cacctgtcta gcggtcagga cctgggtctc gagcaggtgc ttttcaaagc gttccacggg
     5101 gtcccgccga cgccactcct cgatcaacgc cttcggcacg taggaggcgt cgtcggcctc
     5161 ggagtggccg cgaatgcgca tcgtccggca ctcgagaaag gatgggccct gcccctcccg
     5221 cgcccggtcg acggcgcgcc gggccgcgtc gtagacggcg aggatgtcat tcccgtcgac
     5281 cgaacacgcg ggcatgccgt atcccgccat ccgatcgacg aggggaccgg ccacctcgcg
     5341 gctgacgggc gtcgagtagg cgaattgata gttccagcag acgagcacga gcgggagccg
     5401 gagcacggcg gccaggttgg cgccctcgtg aaactccccc gtcgccaggg tgccctcgcc
     5461 gaagaacgcc atcgccaccc gggcttcctg gcgcacggtg aacccgagcg cggcccccgc
     5521 ggccacgggg atgctcgcgc cgatgtggtc gatgtggagg aggatgcggg gcccgaggcc
     5581 tccgatgtgc aggttccctt cgcgtccccg cgtcggcgac ccggtccgcc ccaggaagtt
     5641 cgcgaagtag taccggacgg gcatgccgag gaccagtcgg gcgccgagat cgcgatggag
     5701 ggtggacacc acgtcctggg ggccgagggc agaggcggcc ccgaccgcga tggcctcgtg
     5761 gccgtatcct gtgaagcagg cgccctcgac cgcgccctgg tagtacaact tgacgatccg
     5821 gtcttcggcc agccgttgga ggagcatatg ccggaagagc gtgcgcagat cgtcggccgc
     5881 gaggcccggg ggaaccgcgg cgaggggggc ggccgtgatg tcgcccctac cagcctccat
     5941 aggcgaccca cccgccatcc accgtcacgc acgacccggt gatgtagccg gcgtcatccg
     6001 aggcgaggaa ggccgcgacc ctggcgactt cctcggtggt gccgaagcgt cccgccgggg
     6061 tccgccgctc gatgtcggcg ttcgtatagt ccccggagac ctgatcgcgg tcatccatgg
     6121 ccgtcttgat gtacgccggg tcgatgctca cgacgcgaat gccgtgccgc gcccactccg
     6181 cggcgaggac tttcgtgagg ccgatcaacc cgtgcttgct ggcgcagtag gccgcgcgct
     6241 tcggcaatcc cacctctccc aggatcgacg agatgttgag gatgacgccc ttccgctggg
     6301 gaatcatcac ctgggcggcg gcctgcgcgc agaagaatgc gccggtcaag tttgtgtcga
     6361 tggtgagccg ccacccctcc tcgggcagcg cctcggacgg gccgatcatc gatgtcccgg
     6421 cgttattcac gaggatgtca aggcggttcc accgctgccg ggccgcggcc accagccggt
     6481 ccgcagacgc cttctcgcgc acatccccgg cgaccgcgat cgcgtcgtgg ccttccccgc
     6541 gcagcgcgcc ggccacgcgt tctccccgct cgcgggcgcg cgagttgatg acgatccgcg
     6601 cgccttcccg cgcgtacgtc caggcgatcg cctcgccgat ccccatgctg gatcccgtga
     6661 tcaccgccac gcggtcgcgg agccgcattc ctctcctcct gtgtgcgctg gtcggcgggc
     6721 tacatgcgca cttcgccgtg cgccgccagc aacttggcct tccgtaccgc ttcaatgcgc
     6781 cgttccgcca gcaggtcggc cgcccggtac gtgggaatct tttcctgccg ggagatgcgg
     6841 atgagcgagg ccatcgtctc gtagatccgc gagacgttcg tcatcgcgcg ctcccggttg
     6901 aacccgccgg ggttgagacg gtcggtgtcg aaaatcgtgc cccccgcgtt gacgatatag
     6961 tccggagcat acaggattcc cctggcgtcg atctcctgcc catcccgttc ttctcggagc
     7021 tggttattgg ccgagccgca gatgatcttg accttgaagc gcgggatcgc ctcggcattg
     7081 atgacccccc ccagggcaca ggggcagaag atgtctgcca cccggccgat gatttcgtcc
     7141 gggggcacgg cggggaggga aaaccggctg gtgatcgcct cgaccttctc gcggtcgatg
     7201 tcggtcacga cgacacgcgc cttctccttg accagcagcg gcaccacctc cgacccgacc
     7261 acccccagcc cttgcacggt gaccgtgcgt ccctcgaggg aatcactgtc gtacacggcc
     7321 ttggcacagg ccttcatgcc ctgcaccacc ccgaacgcgg tggccccgcc gatgggcccc
     7381 gcgccgcccc aggaggccgg cagggtcacg acgtacggcg tttccatccg catgtactcc
     7441 atttcccgca gcgtggtccc gacgtcctcg ccggtcatg
//
LOCUS       DASQGH010000433         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41506, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000433 DASQGH010000000
VERSION     DASQGH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41506"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VEW91_08480"
     CDS             complement(<1..354)
                     /locus_tag="VEW91_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYM91653.1"
                     /translation="MPEARVNGVRLHYEIHGGGAPLILAHEFAGDSRSWDPQVKFFAR
                     RYQVITYNARGYPPSEVPEQREAYSQEIAVEDLHGLIQHLQVPPVHLCGLSMGGYAVL
                     HLGLRYPALARSLVVA"
     gene            540..1433
                     /locus_tag="VEW91_08485"
     CDS             540..1433
                     /locus_tag="VEW91_08485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYM91654.1"
                     /translation="MSSTQMLRAGLAAIAVAGLGLPSALSAPPDDNHEIVRITPASPV
                     QGDTLAVVVAARGPAELSVRFDGVGVTAYALADGARRALIGTDPTIAPGSHTVSVIIT
                     EAGTPPVRWARAVQIKPGQFGARTLTLPPATFGLISPENLATERRALGPVLSRRTPEA
                     MWTGAFLAPSSGVMDSPYGMQSMYNGHRTWWHQGVDFAAEAGSPVIAANAGIVALAQA
                     LPLGGNTVVIDHGQGVLTEYLHLSAMTVGPGTRVDRGALIGRIGQTGLVTGPSLHWGL
                     YVNGNWVNPLFWTEPRPDLTP"
     gene            complement(1486..>2531)
                     /locus_tag="VEW91_08490"
     CDS             complement(1486..>2531)
                     /locus_tag="VEW91_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM91655.1"
                     /translation="PVLIVGAGAATPEARAALIQIAEDTGARVYGERLPVRSVFPTDH
                     PQYLGMIGLSLSQLRRELGEADVVLVAGARKFASLLYTPAIQLPKQTRVFHIDTDPWE
                     LGKNIPVTLGVVGDPSAVLPELAMLLRGRIGASGRQEAGARAALVRRERTRREEEWRA
                     AAAPPPPGGRMTQEFVYRTLGEAMDDSVTIVDEAITAARIVDRYLPLRTEQAYLGIAA
                     GSLGLALPASLGAQMAWPDRRVICTVGDGSLMYTVQALWTAARYRIPVTVLVMDNRAY
                     QVLKDGMAQYKGGPVPPERLVGFDLQSPVVDIPTVAQGFGVPARSISRPDELRDALAE
                     RTDGPRLLDVIIA"
BASE COUNT          380 a          877 c          910 g          364 t
ORIGIN      
        1 ggcgaccacg agcgagcggg caagcgcggg gtagcgcagg ccgaggtgga gcaccgcgta
       61 cccgcccatc gacaggccgc agaggtggac gggcggcacc tggaggtgtt ggataagacc
      121 gtggagatcc tccacggcga tctcctggga gtacgcctcc cgctgctccg gcacttcaga
      181 aggcgggtac ccgcgggcgt tgtacgtgat gacctggtag cgtcgggcaa agaacttcac
      241 ctgcggatcc caactccggc tgtcgccggc gaactcgtgg gcgaggatca gcggggcgcc
      301 gcccccgtga atctcgtagt ggagccgcac cccgttgacg cgtgcctccg gcatccctcg
      361 acctcctgca ttccgccaag gggggaattg gccttggtcg catcgcctcc ctcccggctc
      421 gtgcacgagc ggcccaggga ggtgcggtgc aggaatgccc tgcgcccgta tcatacgtcg
      481 ctgcggggaa gatcgcgtct gaacccccag gtcggtgggc atggatcgaa tgacgagcca
      541 tgagtagcac acagatgttg cgcgcgggcc tggcggccat cgcggtggcg gggctggggc
      601 ttccctcagc cctttccgcg ccgccggatg acaaccacga gatcgtcagg atcacgcccg
      661 catcgccggt gcagggggat accctcgcgg tcgtcgtggc ggcccggggc cctgccgaac
      721 tgagcgtgcg gttcgatggc gtgggcgtga cggcgtacgc gcttgcggat ggggcgcgca
      781 gggcgctgat cgggaccgac ccgaccatcg cgccgggctc ccacacggtt tccgtcatca
      841 tcaccgaggc gggaaccccg cctgtccggt gggcgcgagc cgtgcagatc aagcccgggc
      901 agttcggggc ccgcacgctg acgctgccgc cggcaacgtt cgggttgatc tcaccggaga
      961 acctcgcaac cgagcggcgc gcgctcggcc cggtgttgag ccgccgcacg ccggaagcga
     1021 tgtggacggg agcgtttctg gccccctcca gcggggtgat ggactccccg tacggcatgc
     1081 agagcatgta caacggccac cgaacgtggt ggcatcaggg cgtcgacttt gccgccgagg
     1141 cgggctcgcc ggtcatcgcc gccaacgcgg ggatcgtggc gctcgcccag gccctgccgc
     1201 tcggcggaaa cacggtggtg atcgatcacg gccagggcgt gctcaccgag tacctgcatc
     1261 tgtcggcgat gacggtcggc cccggcacgc gcgtcgaccg gggggccctg atcggccgga
     1321 tcggccagac gggcctggtg accggtccca gcctccactg gggcctgtac gtgaacggga
     1381 actgggtgaa cccgctgttc tggacggaac cacgccccga tctgacgccc tgaccaccag
     1441 cgccgccttc gaggtctgag ccgccggggc tctcgccggc ggcgatcacg cgatgatgac
     1501 gtccagcagc cgcggaccgt cggtccgctc cgcgagggcg tcccggagtt cgtccggccg
     1561 ggagatggac cgcgccggga ccccgaatcc ctgggccacg gtggggatgt ccaccacggg
     1621 tgattggagg tcaaatccga ccaaccgctc cggaggaacc gggccgcctt tgtactgggc
     1681 catgccgtcc ttcagcacct ggtaggcgcg gttgtccatc accaaaactg tcacgggaat
     1741 ccggtaccgg gccgcggtcc acagcgcctg gaccgtgtac atgagcgagc catcgccgac
     1801 cgtgcagatc acccgccggt ccggccacgc catctgcgcc cccaggctgg cgggaagggc
     1861 aagcccgagc gatcccgcgg cgatcccgag gtacgcctgc tcggtgcgga gcggcagata
     1921 ccggtcgacg atgcgggcgg cggtgatggc ctcatccacg atggtgacgg agtcgtccat
     1981 cgcctccccc agggtgcggt agacgaactc ctgggtcatc cgccccccgg ggggcggggg
     2041 cgcggcggcg gcccgccatt cctcctcgcg ccgggtccgc tcccggcgga ccagagccgc
     2101 gcgggcgccg gcttcctgcc tgcccgacgc gccgatgcga ccgcggagca gcatcgcgag
     2161 ctccggcagg acggcggatg ggtcacccac gacgccgagg gtcacgggaa tgttcttgcc
     2221 gagctcccac gggtcggtgt cgatgtggaa caccctggtc tgcttcggca gctggatcgc
     2281 gggcgtgtac aggagcgagg cgaacttgcg cgcccccgcg acgaggacga cgtcggcctc
     2341 tcccagttcc cggcggagtt gtgagagcga cagcccgatc atccccaggt actggggatg
     2401 atcggtggga aacaccgagc gcacaggcag ccgctccccg tagacccggg ccccggtatc
     2461 ctcggcgatc tggatcaggg ccgcgcgcgc ctcgggggtc gccgcgcctg cgcccacgat
     2521 gagcaccggg c
//
LOCUS       DASQGH010000434         6882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10319, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000434 DASQGH010000000
VERSION     DASQGH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10319"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1632
                     /locus_tag="VEW91_08495"
     CDS             178..1632
                     /locus_tag="VEW91_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HYM91656.1"
                     /translation="MTDATVPSRHPRGAPAAGSASGLARVGLALADWCERWFPDAFVF
                     AMAAVVIVAIGALLLGASLQAVMVQFGKGFWDLITFTMQMALIIIGGYVVAVSPPIAR
                     LIESLARVPKTGRGAIAYIAGVSMLASMISWGFSLIFSGLLVRALARRQPRMDYRAAG
                     AAAYLGLGSIWALGLSSSAALLQANPASLPPALAKITGVIPFTETIFLWQGLATALIL
                     LIVSVAIAFWSAPSEAAAHPASALGVTFQPMRQELSPRQHPSEWLEYSPMLTILIVLL
                     GVGWLVGTFATAGPLVAISNLNTYNFIFLMAGLLLHWQPRRFLQAVTSSVPATAGVLI
                     QFPFYAGIAAILTGAANPAGVTLSNWLAHLFVSVSTQRTFPLLASAYSAVLGLFVPSG
                     GGKWIIEAPYIMQAANSLHVHLGWVVQIYNAAEALPNLVNPFWMLPLLGVLGVKAREL
                     VGYSLLQLIVHVPLVFFLMWLFAMTLPYHPPMGP"
     gene            complement(1726..2757)
                     /locus_tag="VEW91_08500"
     CDS             complement(1726..2757)
                     /locus_tag="VEW91_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYM91657.1"
                     /translation="MTQRELVGRTVTVLAVVILAWLLVWVVRQMTEILILLLVSAILA
                     SGLAPLVGLVERRKVSRGVAIFVLYLAIFAAVLLIFSIILLPAVNESSRFIQQLPQLT
                     EHIRVWLAGLHSRWPWLPDLAGILDRVPSLATALSRLGPQAAGVAFRFLGGLAAVITV
                     LVFTFYMLLEGAEIKKGFIALFPAAERRRVNLVLDRIGAKFGGWLRAQLLLSFSVAVP
                     VALGLSLLRMPYPFLLGIVAGIGELIPMVGPTLGAAVAVLVALSQQPWQLVGVLIFYT
                     IVLNVEPHILVPRIMARVVGMSPILTLVALLTGIKLLGILGGLLAVPVAAALQVIVAE
                     VAEEVLPRS"
     gene            complement(2831..4099)
                     /locus_tag="VEW91_08505"
     CDS             complement(2831..4099)
                     /locus_tag="VEW91_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYM91658.1"
                     /translation="MPLNLSNVNTEFLLFMERVAISLGIGMMIGMEREWAHKDIGVRT
                     FSIVAVAFTLAWGISPTTSYILLLALIPLITLVNWRSFVRDRSLELTTSVALLATAIL
                     GLLVGQGQLLVAAACAVVMTGLLAWKAEVVKFAGEVTIEEIRGALLLGMIALVVYPLL
                     PKGSIDPWHLIDLQAAWTVVLVISAIAFANYVLLRLYGSRGIRWTGLLGGLVNSTATV
                     AELASRARRDPAHLSIFALLGMLTANSAMLIRNGLILGIFARQALAYGWISVALMLLA
                     SVIVTSSIHVEEGATAPLRIQSPISVRHALTFGGLFVLITLAAALANRELGQTGFLLV
                     ATLGGLVSSASTSAAAGILAANGALPPDLAGYGVVLTSMASLAFHVPMAQMAGKNNTV
                     TMRLARFSILILAAGIAGLLAQTLLLPHRL"
     gene            complement(4112..5299)
                     /locus_tag="VEW91_08510"
     CDS             complement(4112..5299)
                     /locus_tag="VEW91_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM91659.1"
                     /translation="MRSRVGHRGLIVLFMAIFTVMMGFGIVLPVLQFYAREVGATPVQ
                     IGLLATSYAFMQFLFAPLWGALSDRIGRKPVFALGLLGYAVSFIIFGLSHQVWELFMA
                     RILGGVLSAATLPTAMAYIADTTTDEGRGSGMGLMGAAMGLGFTIGPGIGGILGRLNL
                     ALPFFVGAGLALFTLAMSWGALPEPARRGLRAPRPSRIDAIRLALGGPLALFYIVTLV
                     GAFALAGLEATYALFAQDRLRLSSAAGAGAIGIVFVVVGLVQASLLGGVVGRLINRWG
                     EDRLVHGGLLLAAVGYLLIVLTNSIATLAVYAAIAGAGHALMRPSIASLISKRTAAGQ
                     GLSIGIMDSFDSLGRVIGPAWGGWVYHAGISLPYASAGLLLLLTAGVSVAAGARGVPV
                     QAN"
     gene            5401..5475
                     /locus_tag="VEW91_08515"
     tRNA            5401..5475
                     /locus_tag="VEW91_08515"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5433..5435,aa:Asn,seq:gtt)
     gene            complement(5654..5944)
                     /locus_tag="VEW91_08520"
     CDS             complement(5654..5944)
                     /locus_tag="VEW91_08520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HYM91660.1"
                     /translation="MELFIFARFHARAGEEQGVEEAIRDVAGPTREEPGCLSYHVFRS
                     TRDPRLFYIHSRWRDEAAFDTHADLPHTVRFLERVEPLIDHPLDVTRAKLVV"
     gene            complement(6100..>6882)
                     /locus_tag="VEW91_08525"
     CDS             complement(6100..>6882)
                     /locus_tag="VEW91_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYM91661.1"
                     /translation="VSGMHPLLGATFMAATVSISVPVAVITLNWFATLWQARGRIRLT
                     TPMLFAIGFLSLFVTGGITGLFLADAPSDIYLHDTYFVVAHFHFVMAASAVFAVFAGT
                     YYWFPKMFGRMMNETLGKIHFVATFVTIYTTFFPMHFLGIHANSRRLYSPTFYPFLSS
                     VQPLDVFISLSAFVLGAAQLVFIANFFWSLTRGALAEANPWRANTLEWTVVSPPPHGN
                     WGDNTPEVYRGPYDYSFPEATDDYLPQTVPPVPPGVAPHGDD"
BASE COUNT         1246 a         2278 c         2330 g         1028 t
ORIGIN      
        1 ccgaagtcta gtgcgtatga tgcgggcgca ggatgcgcac ggcggtgtgg cgaatggcaa
       61 tcccgatcat ctgtcgttgg gactgtcttg attcactttg gtgatcgtct ttgagacccc
      121 tcgcctcgcg acctggacag cgcgatccaa aatagcatat acgagaggag gtttgggatg
      181 acggatgcca ccgttccatc ccgacaccca cggggagccc cggcggcggg aagcgcgagc
      241 ggcctggcga gggtcggttt ggcgctcgcc gactggtgtg aacgctggtt ccccgacgcc
      301 ttcgtcttcg ccatggcggc ggtggtgatc gtcgccatcg gcgccctgct gctcggagcc
      361 tcgctccagg cggtgatggt gcagttcggc aagggcttct gggacctcat caccttcacg
      421 atgcagatgg ccctcatcat tatcggaggc tacgtggttg cggtctcgcc gcccatcgcg
      481 cggctcatcg agtcgctcgc cagggtgccc aagaccggcc gcggcgccat cgcctacatc
      541 gccggggtga gcatgctcgc ctcgatgatc tcgtggggat tcagcctcat cttctccggc
      601 ctgctggtgc gggcgctcgc ccggcgccag ccgcgcatgg attaccgggc ggccggcgcc
      661 gcagcgtacc tgggcctggg gagcatctgg gcgctcggct tatcgtcgtc ggccgcgctg
      721 ctgcaggcca acccggccag cctcccgccc gcgctcgcga agatcaccgg cgttatcccc
      781 ttcaccgaaa cgatctttct ctggcagggg ctcgccacgg ctctcattct gctgatcgtc
      841 tcggtcgcca tcgccttctg gtcggcaccc tccgaggccg ccgcccatcc ggcctcagcc
      901 ttgggcgtga ccttccagcc gatgcgccag gagctgagcc cgcgccagca ccccagtgag
      961 tggctggagt acagtccgat gctgaccatc ttgatcgtcc tgcttggggt gggatggctg
     1021 gtgggcacct tcgcgacggc cgggccgctc gtcgccatct cgaacctcaa cacctacaac
     1081 ttcatcttcc tgatggccgg cctgctgctc cactggcagc cgcggcggtt ccttcaggcg
     1141 gtaacgtcgt cggtaccggc cacggccggc gtgctcatcc agttcccgtt ctacgccggg
     1201 atcgcggcga tcctcaccgg cgccgccaac ccggcgggcg tgaccctctc caactggctg
     1261 gcgcacctct tcgtcagcgt ctccacccag cgcacgttcc cgctgctggc ctcggcctac
     1321 tcggccgtcc tcgggctctt cgtgccctcc ggcggcggaa agtggatcat cgaggcgccc
     1381 tacatcatgc aggcggccaa ctcgctccac gtccatttgg ggtgggtggt tcagatctac
     1441 aacgcggccg aggcgctgcc gaacctggtc aatccgttct ggatgctgcc gctgctgggg
     1501 gttttgggcg tgaaggcgcg cgaactcgtg ggctactcgc tgctgcagct catcgtccac
     1561 gtgcctctgg tgttcttcct catgtggctg ttcgccatga cgcttcccta tcacccgccc
     1621 atgggtccgt aatcggccgg cgcttgggga ttaccaccgg gggagtcgcc gctcgggggg
     1681 atcgccgcgc cgtcacggcg cggcgggcga gccggccggc tgcggtcagg accggggcag
     1741 cacctcctcc gcgacctcgg ccacgatgac ctgaagcgcc gcggcgaccg ggaccgcgag
     1801 cagcccgccc aggatcccga gcagcttgat gcccgtcaaa agcgcgacga gggtcaggat
     1861 cggggacatg ccgacgacgc gcgccatgat acgcggcacg aggatgtggg gctcgacgtt
     1921 caggacgatg gtgtagaaga tcaggacgcc caccagttgc cagggctgct gcgagagggc
     1981 gaccaggacg gccaccgcgg cgccgagcgt gggccccacc atcgggatca gctccccgat
     2041 cccggcgacg atgcccagga ggaaggggta gggcatccgg aggagggaga ggcccagcgc
     2101 cacggggacc gcgacggaga aggagaggag caactgcgct cgcagccacc cgccgaactt
     2161 cgccccgatc cggtcgagca ccaggttgac gcgccggcgc tccgcggccg ggaacagagc
     2221 aatgaaccct ttcttgatct ccgcgccctc gagcaacata taaaaggtaa acacgagcac
     2281 cgtgatgacc gcggcgaggc cgccgaggaa gcggaacgcg acacccgccg cctgaggccc
     2341 cagccgggag agagcggtcg ccagcgacgg cacgcggtcc aggatgcccg ccaagtccgg
     2401 cagccacggc caccggctgt gaaggccggc aagccagacc cggatgtgct cggtcagttg
     2461 cgggagttgc tggatgaacc tgctggattc gttcaccgcc gggaggagga tgatcgagaa
     2521 gatcaggagg acggccgcaa agatcgccag gtacaggacg aagatcgcca cgccgcggga
     2581 gaccttccgg cgctccacca gcccgacaag gggggccagc ccggacgcga ggatggccga
     2641 gacgaggagc aggatgagga tctcggtcat ctggcggacg acccacacca ggagccacgc
     2701 cagaatgaca acggccagga ccgtgacggt ccggccaacc agttcccgct gggtcacggt
     2761 gccagccctg gggtcattgt cgccgcaacg ttcgcggcgg aaaccgggag tcctgtcgcg
     2821 gccgcgcggg ttagaggcgg tgcggcagga gcagggtctg cgccaggagt ccggcgatcc
     2881 cggcggcgag gatcaggatg gagaagcgcg ccagacgcat ggtgaccgtg ttgttctttc
     2941 cggccatctg agccatcgga acgtggaacg ccaggctcgc catggaggtg aggacgaccc
     3001 cgtacccggc gagatccggg ggaagcgccc cgttcgcggc caggattccc gccgcggcgg
     3061 aagtcgacgc gctgctgacc agccccccga gcgtggccac gaggaggaaa ccggtctggc
     3121 cgagctcgcg gttcgccagg gccgccgcca gcgtgatcag gacgaacagc cccccgaacg
     3181 tgagcgcgtg gcgaaccgag atgggcgatt gaatgcggag gggcgccgtc gccccctcct
     3241 cgacgtggat gctgctcgtc acgatgacgc tcgccagcag catgagggcg acggagatcc
     3301 acccgtacgc cagcgcctgc cgggcgaaga tcccgaggat caggccgttg cggatcagca
     3361 tggcgctgtt ggcggtgagc atgccgagga gggcgaagat cgacaggtgc gccggatccc
     3421 tgcgcgcgcg gctggccagc tccgccaccg tggcggtgct gttgacgagg ccgccgagca
     3481 ggccggtcca acggatgccc cggctgccgt agagccggag caggacgtag ttcgcgaagg
     3541 cgatcgcgga gatcacgagc acaacggtcc acgccgcctg aaggtcgatc aggtgccagg
     3601 gatcaatcga tcccttgggc agcagcgggt agaccaccag cgcgatcatc cccagtaaga
     3661 gcgcgccccg gatctcctcg atagtcacct cgccggcgaa cttgacgacc tcggccttcc
     3721 aggccaggag ccccgtcatg accaccgcgc acgccgccgc cacgagcaac tgcccctgcc
     3781 cgacgaggag cccgaggatc gccgtggcga gcagcgccac cgacgttgtc agttccaggg
     3841 agcgatcccg gacgaagctc cgccagttga ccagggtgat gaggggaatg agcgcgagca
     3901 gcaggatata gctggtggtc ggcgagatcc cccaggccag cgtgaacgcc accgcaacaa
     3961 tcgagaacgt gcgcaccccg atgtccttgt gggcccattc gcgctccatc ccgatcatca
     4021 tgccgatccc cagcgagatc gccacccgct ccatgaagag gaggaactcg gtgttgacgt
     4081 tgctgaggtt gagcggcatg gtctgtgcgg ctcagtttgc ctggacgggc actccgcggg
     4141 ccccggccgc gaccgagacg cccgcggtga gcaagagcaa gagccccgcg ctcgcatagg
     4201 gaagactgat gccggcgtgg tacacccacc cgccccaggc cggcccaatg acacggccca
     4261 ggctgtcgaa ggagtccatg atgccgatgg aaaggccctg gcccgctgcg gtccgcttcg
     4321 agatgaggga cgcgatcgac ggccgcatca gggcgtgccc ggcgcctgcg atcgccgcat
     4381 agacggccag ggtggcgatg ctgttggtga gcacgataag aagataccca actgccgcca
     4441 gcagcaggcc tccgtggacc agccggtcct ctccccacct gttgatgagc cggccgacca
     4501 ccccgccgag aagactggcc tggacgaggc ccaccaccac aaagacgatg ccgatcgctc
     4561 cggcccccgc ggcggagctc aggcgcagcc ggtcctgggc gaacaacgca tacgtggcct
     4621 cgagccccgc gagagcgaac gcgcccacga gcgtgacgat gtagaacagc gccagcggac
     4681 ctccgagcgc gagccggatc gcatcgatcc gggaggggcg gggggcccga aggccgcggc
     4741 gcgcaggctc gggaagcgct ccccagctca tcgccagggt aaacagggcc agcccggcgc
     4801 ccacaaagaa cgggagggcg aggttcaggc ggccgaggat gccgccgatc ccggggccga
     4861 tggtgaaccc aagccccatc gccgcgccca tcaggcccat gccgctcccc cggccttcgt
     4921 ccgtggtagt gtccgcgatg taggccatcg cggtggggag cgtggcggcg ctgaggaccc
     4981 cccccaggat ccgcgccatg aacagctccc agacctggtg cgagaggccg aagatgatga
     5041 acgagacggc gtagccgagg agccccaggg cgaagaccgg tttgcggccg atgcggtcgc
     5101 tcagcgctcc ccagagcggc gcaaacagaa actgcatgaa ggcgtagctc gtggccagca
     5161 ggccgatctg gaccggggtc gcgccgacct cgcgcgcgta gaactgcagg accgggagga
     5221 cgatcccgaa ccccatcatg acggtgaaga tcgccatgaa cagcactatg agcccacggt
     5281 ggcccacgcg cgagcgcatt ggaggacacc attatccccg cctgggtgtc ggcctgtcaa
     5341 ggcgccgccc atcgccacct tgacccgcgg cgggcgaatc cgtagaatca atgcggacga
     5401 tcctcggtag ctcaacggta gagcatccgg ctgttaaccg gagggttgta ggttcgaatc
     5461 ctacccgagg agccaatgat taaggtccag caagcgattc ctagcgcgcg gcccccttgg
     5521 aagtggcaga aactagaggt aaacgcttcg gtgcgcaaag ggcgcgggga tgaccgttaa
     5581 tctgacattt ccatcttgga gcgatcatcc ccggtgtcgc cctccggcgg agggaccgtg
     5641 ctcctcgacg acctcaaacg acgagcttgg cgcgcgtcac gtcgagcgga tgatcgatca
     5701 gtggctccac ccgctcgagg aagcgcaccg tatgcggcag gtccgcgtgc gtgtcgaacg
     5761 cggcctcatc cctccagcgc gaatggatgt agaagagcct cggatcccgg gtcgagcgaa
     5821 acacgtgata gcttaggcag cccggttcct cgcgggttgg acccgcgacg tcgcggatcg
     5881 cctcctcgac cccctgctcc tcgcctgcgc gcgcgtgaaa tcgcgcaaag atgaacagtt
     5941 ccatcccgtc tccttggtgg aaaagcatct gaggggtcgc gctccgcaca cagtggggcc
     6001 agaaaatgac cggcctcttg tgagttcacc ccgggattgc cacgataggg tcccgcctga
     6061 tcctcagtat acacgcagag gacccgggta gcggttaagt taatcatcgc catggggcgc
     6121 caccccgggc ggaaccggcg gcaccgtctg cggaaggtag tcgtcggtcg cttccgggaa
     6181 actgtaatcg tagggcccgc ggtagacctc cggtgtattg tctccccagt tgccgtgcgg
     6241 cgggggagag accaccgtcc actccagggt gttcgcccgc cacgggttgg cctccgcgag
     6301 ggcaccgcgc gtgaggctcc agaagaagtt cgcgatgaag accagctgcg ccgcgcccag
     6361 cacgaacgcg ctcaacgaga tgaagacgtc caggggctgg acgctggaga ggaacgggta
     6421 gaaagttggc gagtacaatc gtcggctgtt ggcgtggatg ccgagaaagt gcatcgggaa
     6481 aaacgtggtg taaatggtga caaacgtcgc cacgaagtgg atcttcccca gcgtctcgtt
     6541 catcatccgg ccgaacatct tggggaacca gtagtacgtc cccgcgaaaa cggcgaacac
     6601 cgccgaggcg gccatcacga agtggaagtg ggccacgacg aagtaggtgt cgtggaggta
     6661 gatgtccgac ggcgcgtcgg ccaggaagag gccggtgatc ccgccggtca cgaacagcga
     6721 caggaacccg atggcgaata acattggggt cgtcaggcga atccgccccc gcgcctgcca
     6781 gagcgtggca aaccagttca acgtgatcac ggccaccgga acgctgatgc tgaccgtggc
     6841 ggccatgaac gtcgctccca ggagcgggtg catcccgctg ac
//
LOCUS       DASQGH010000435         6421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52778, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000435 DASQGH010000000
VERSION     DASQGH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52778"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VEW91_08530"
     CDS             complement(<1..1072)
                     /locus_tag="VEW91_08530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HYM91662.1"
                     /translation="MTLRILHTADVHLGATFRGLGERGREQRRQVEATFARVVRLALD
                     ERVDVVVIAGDLFDSVAAARAHLAGAAEELRRLEAAAIPVCVIAGNHDPLGDGSTSVW
                     GDLAAQASVTVFGPQLESRVFPALDLTVVGCSRQRHLSAHSPLGGLPVARQTRFLVAL
                     AHGSVERPDLPAQFGQITREEIARSGVDYLALGDWHSTRDVSGGGVAAWYSGAPEMID
                     LDEPESGNVCLVTLEAPGKVEVARPRVGRRRGRRLVLDVATLGGAEAVERAIRAEADP
                     DLALTVVLTGLASLTDRMSAEPLLEDLAGEFFRLRIQDESHVRPDVIDPGRFPENTVL
                     GRFVRMMQAEVAGREGEARAPAG"
     gene            complement(1069..4104)
                     /locus_tag="VEW91_08535"
     CDS             complement(1069..4104)
                     /locus_tag="VEW91_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYM91663.1"
                     /translation="MADRRDDRVRHDQLALALAGPDGAIVRASPPTVPAARAVPSAGE
                     AILDGLTEEQLAAVTHAEGPLLIVAGAGTGKTAVITRRIAHLITTRRARPEQILALTF
                     TDRAAAEMEERVDRLVPYGYTQAWISTFHAFGDRVLRENAVHLGLSPDFRVLTQAEQI
                     IFVRERLFELPLEYFRPLGDPTRHIAALAALFSRAKDEDVTPEEYTAYAERVAAEPAG
                     DPAASEQVRQQRELASAYQVYQRLLTREGRLDFGDLITLTLRLLREHPTVLQRYREQF
                     QYILVDEFQDTNYAQFELVKLLAGDRRCLAVVADDDQAIYRWRGASYSNITYFTQAYP
                     DARTVVLTHNYRSTQLILDSAYRLIRHNDPDRLEVRHQIDKRLQATSGVGILPRHLHF
                     DTLHSEADGVADRIAQRVAAGQWRYSDVALLVRANRDADPFLRALNLKGIPFVFSGTR
                     GLYDREEIRLIVAALRCIASPHDSLSLYLLSTAPFYDVPAQDLADCMGYAHRKHRSLS
                     HVYRHLGAIEELEVSPEGRATIAKLVEDLEAMTQLASRYPTGRVLYEYLVTRTGYVGR
                     LTASGSPEDEHRVANIARFFDLVSRYGEIAPVDRVPGFVGYLDVLIEAGDDPPTAEFE
                     TDADAVRVLTIHKAKGLEFPVVFMVGLVVDRFPSRARTEPLPLPDALMKDLLPTGDFH
                     LEEERRLFYVGMTRAKRDLYLTSGRDYGGARPRKVSRFVLEALDLPRADPETFRASVV
                     QAVERHAPPVDLHPQLMLVPSGDQVVPVSFRQVDDYSTCPLKYKYIHILRVPVLRDHR
                     VVYGAALHEAVQEYNRRKARHQPVTAEDLIAHFERAWVNEGFLSREHEDQRLEAGRNA
                     LRGFFEYQETSRAVPTFVEREFRFPIGPTILRGRWDRVDIRGGEAVIIDFKSTDVRTQ
                     QDADRRARDSEQLAIYTLAYKEVFGRLPARVELHFLGREVVVGRARKTEVDLHRTREM
                     ILHAADGIRAQRFIATPDPYRACPYCVFNQICPYTATAE"
     gene            complement(4097..4801)
                     /locus_tag="VEW91_08540"
     CDS             complement(4097..4801)
                     /locus_tag="VEW91_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HYM91664.1"
                     /translation="MPLITDIRPPGASRGQTGPGPLKAGGEWRLVVLDDGRRLRVDLE
                     QVARHGLEPGEAVGTRLIAGLEARDAYLRARERALRLLAIRPRSAEELRQRLLQERAP
                     RGAIRAVIATLAGQGYLDDLAFARSWIAHRIAAGACAAARLRWELRQKGVSTIVIDRA
                     LQERFGAGADRARVEEQSAIALVSRRLPAYRHLTRERQVRRLAGLLERRGFAPATIAR
                     ALRVIGRADVPEASDG"
     gene            complement(4812..5927)
                     /gene="recA"
                     /locus_tag="VEW91_08545"
     CDS             complement(4812..5927)
                     /gene="recA"
                     /locus_tag="VEW91_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009069008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HYM91665.1"
                     /translation="MNERQRALDLALTQIERQFGKGSIMKLGENQAKLSVDVIPTGVI
                     ALDVALGVGGVPRGRVVEIYGPEASGKTTLGYHIIAEAQREGGVAAFIDAEHALNTEY
                     ARNVGVDVDNLLISQPDSGEQALEIGEMLVRSGAIDVIVVDSVAALVPRAELEGEMGD
                     AHVGLQARLMSQALRKLVGTISKSRTTVVFINQLREKIGIMFGNPETTTGGRALKFYA
                     SVRMEIRRTESIKSGDQVIGQRVRIRVVKNKLAAPFRDAEADIIFPRGISKVGSLLDL
                     AVAQNFVTRTGTWFAYKDMRLGQGRDNAREFLENNPELAREIEARTREKLGIARPAPQ
                     APSEARATPAGGETGPLPRDARSGAPVTKGMPARSSR"
     gene            complement(6016..>6421)
                     /gene="thpR"
                     /locus_tag="VEW91_08550"
     CDS             complement(6016..>6421)
                     /gene="thpR"
                     /locus_tag="VEW91_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYM91666.1"
                     /translation="GRVAAAARGVAERTKRFEIMLAGLGAFPSPRRPQVVWVGVGEGA
                     DRLIALARDLDTALHRLKFPLEDRPFQPHLTVARVRRAGPVPDLSGPLHDLDGMILGT
                     QTVDALLVMESTLHPSGATYRPVDTVWLREVP"
BASE COUNT          959 a         2298 c         2218 g          946 t
ORIGIN      
        1 ctccggcggg cgcgcgcgcc tccccctccc gcccggcgac ctccgcctgc atcatccgga
       61 caaaccggcc gagcaccgtg ttctccggga accggccggg gtcgatcacg tccggacgca
      121 cgtgcgactc gtcctgaatc cggagccgga agaactctcc ggccaagtcc tccaggagcg
      181 gctcggcgct catgcgatcg gtgagcgacg ccagcccagt gagcacaacg gtcagcgcga
      241 gatcggggtc cgcttcggcg cggatcgccc gctcgaccgc ctccgctccc ccgagggtgg
      301 ccacatccag caccaggcgc cgtccccggc ggcggccgac ccgagggcgg gcgacctcga
      361 ccttcccggg ggcctccagg gtgaccaggc agacgttgcc ggactcgggt tcgtcgaggt
      421 cgatcatctc gggcgcgccg ctgtaccagg ccgccacccc gccgccggac acgtcgcggg
      481 tggaatgcca gtccccgagg gcgaggtagt ccaccccgct ccgcgcgatc tcctcacggg
      541 tgatctggcc gaactgggcg ggcagatcgg ggcgctcgac gctcccgtgg gcgagcgcga
      601 ccagaaagcg tgtctgccgc gccaccggca gcccgccgag ggggctgtgc gccgagaggt
      661 ggcgctgccg cgagcagccg acgaccgtga ggtcgagggc gggaaagacc cgggattcga
      721 gttgggggcc gaacaccgtc acggacgcct gggcggcgag atcgccccag acggaggtac
      781 tcccgtcccc caggggatca tggttgccgg cgatcacgca cacgggaatc gccgcggcct
      841 ccagccggcg gagctcctcc gcggccccgg cgagatgcgc gcgggccgcg gccaccgagt
      901 cgaagagatc gccggcgatc accacgacgt ccacgcgctc atcgagcgcg agccgcacga
      961 cccgggcaaa cgtggcttcc acctggcggc gttgctcgcg gccgcgttcg ccgagtccgc
     1021 gaaacgtggc gcccaggtgc acgtcggcgg tatgcaggat gcgcagggtc attcggccgt
     1081 cgcggtgtac gggcagatct gattgaagac gcagtacggg cacgcccggt acgggtccgg
     1141 cgtcgcgatg aaccgttggg cgcggatccc atccgcggcg tggaggatca tctcccgggt
     1201 ccggtggagg tcgacctccg tcttgcgggc ccgcccgacc accacctcgc ggccgagaaa
     1261 gtgcagttcc acccgggcgg ggagccgccc gaacacctcc ttgtacgcca gcgtgtagat
     1321 cgccagttgc tcgctgtccc gggcccgccg gtcggcgtcc tgctgggtcc ggacgtcggt
     1381 gctcttgaag tcgatgatca ccgcctcgcc ccctcggata tccacccggt cccagcggcc
     1441 ccggaggatg gtcggcccga tggggaaccg gaactcgcgc tccacgaacg tcgggacggc
     1501 acggctggtc tcctggtact cgaagaaccc ccggagggcg ttccggccgg cctcgaggcg
     1561 ctggtcttcg tgctcccggc tcaagaaccc ctcgttgacc cacgcccgct cgaagtgggc
     1621 gatcagatcc tccgccgtca cgggctggtg ccgcgccttc cggcggttgt attcctggac
     1681 ggcctcgtgg agcgccgccc cgtagaccac ccggtggtcg cgcagcacgg ggacccgcag
     1741 aatatggatg tacttgtact tgagcgggca cgtcgagtag tcgtccacct gccggaagga
     1801 caccggcacg acctggtcgc ccgagggcac gagcatgagc tgggggtgga gatccaccgg
     1861 cggggcgtgc cgctccaccg cctggaccac cgaggcccgg aacgtctccg gatccgcccg
     1921 cggcaggtcg agggcttcca gcacgaaccg gctgaccttc cggggccgcg cgccgccgta
     1981 gtctcggccg ctggtcaggt acaggtcccg cttggcgcgc gtcatgccca cgtaaaacag
     2041 ccggcgctcc tcctcaagat gaaaatcgcc ggtcggcagc aggtctttca tgagcgcatc
     2101 gggcaggggg agcggctccg tgcgcgcacg ggacgggaac cgatccacca cgaggccgac
     2161 catgaacacc acggggaact ccagcccctt cgccttgtgg atcgtcagga cgcgcacggc
     2221 gtccgcatcg gtctcgaact ctgccgtcgg cggatcgtcc ccggcctcga tcaggacatc
     2281 cagatatccg acgaacccgg ggacgcggtc cacgggggcg atctccccgt accgcgacac
     2341 gaggtcgaag aaccgcgcga tgttggcgac ccggtgctcg tcttccgggg agccggaggc
     2401 ggtcaggcgg cccacgtagc cggtgcgcgt caccaggtat tcgtagagga cgcgaccggt
     2461 gggataccgc gaggccagct gggtcatcgc ctcgagatcc tcgacgagct tggcgatcgt
     2521 ggcccggccc tcggggctca cctcgagctc ctcgatcgcc ccgagatggc ggtagacgtg
     2581 gctgagcgac cggtgcttcc ggtgcgcata ccccatgcag tccgcgagat cctgcgcggg
     2641 gacgtcgtag aagggcgccg tgctgagcag gtacagcgac aggctgtcgt gggggctcgc
     2701 gatgcaccgc agcgccgcga cgatgagccg gatctcctcc cggtcgtaga ggccccgggt
     2761 gcccgaaaac acgaatggaa tccccttcag gttgagggcg cgcaggaacg gatcggcatc
     2821 gcggtttgcc cggaccagca gggccacgtc gctgtaccgc cactggcccg cggcgacccg
     2881 ctgggcgatc cggtccgcca cgccgtcggc ctcgctgtgg agcgtatcga agtggaggtg
     2941 ccgggggagg atccccacgc ctgatgtggc ctgcaggcgt ttgtcgatct ggtggcggac
     3001 ctccagacgg tcgggatcgt tgtgccggat caggcggtac gcgctgtcca agatgagctg
     3061 cgtcgagcgg tagttgtgcg tgaggacgac ggtccgggcg tcggggtagg cctgggtgaa
     3121 gtaggtgatg ttgctgtagg acgccccgcg ccaccggtag atggcctgat cgtcatcggc
     3181 gacgacggcg aggcaccgcc ggtcgccggc cagcagcttg acgagctcga actgggcgta
     3241 gttggtgtcc tgaaactcat ccacaaggat gtactggaac tgctcccggt atcgctggag
     3301 caccgtcggg tgctcccgga gcaaccgcag cgtcagcgtg atgaggtcgc cgaaatccag
     3361 ccgaccctcg cgggtcagga gccgctggta gacctgatac gccgaggcga gttctcgctg
     3421 ctggcgcacc tgctcactgg cggcgggatc ccccgcgggc tcggcggcca cccgctcggc
     3481 gtaggcggtg tactcttccg gggtgacatc ctcgtccttc gcccggctga acagcgccgc
     3541 gagagcggcg atatggcggg tgggatcgcc caacgggcga aaatactcga gggggagctc
     3601 gaacagccgc tcacgcacga agatgatctg ctcggcctgg gtcagcaccc ggaagtccgg
     3661 ggagagcccc agatggacgg cgttctcgcg gagcacgcga tccccaaagg cgtgaaacgt
     3721 gctgatccac gcctgtgtgt acccgtacgg caccaggcga tccacgcgct cttccatctc
     3781 cgccgccgcc cggtccgtga aggtgagcgc gaggatctgc tccgggcggg cgcgccgcgt
     3841 cgtgatcagg tgggcgatcc gccgggtaat caccgcggtc ttccccgtcc ccgctcccgc
     3901 cacgatcagc aaggggccct cggcatgggt cacggcggcc agctgctcct ccgtcagccc
     3961 gtcgaggatc gcctccccgg cggacggcac cgcccgggcg gcggggaccg tcggtgggct
     4021 cgcgcgcacg atcgcgccgt ccggccccgc gagggccagg gcgagctggt cgtgcctgac
     4081 ccgatcgtct ctccggtcag ccatcggacg cctcggggac gtccgctctc ccgatcacgc
     4141 gcagggcgcg tgcgatggtc gcgggcgcaa acccgcgccg ctccaggagg ccggcaagcc
     4201 ggcgcacctg ccgctcccgg gtcaggtgcc ggtacgcggg gagccggcgg ctgaccaggg
     4261 cgatggcgga ctgctcctcg accctcgcgc gatcggcgcc cgcgccgaac cgctcctgga
     4321 gcgcccggtc gatcacgatc gtcgagaccc ctttctgccg gagctcccag cggaggcgcg
     4381 ccgcggcgca cgcgcccgcg gcgatacggt gcgcgatcca tgagcgggca aaggccaggt
     4441 cgtccaggta tccctgcccc gcgagggtgg cgatcaccgc ccggatggcg ccccggggcg
     4501 cccgctcctg cagcaatcgc tgccggagct cctcggcgct cctcggccgg atggccaaaa
     4561 gacgaagggc ccgctcccgg gcccggaggt atgcatcgcg cgcttcgagt cccgcgatga
     4621 ggcgcgtccc gaccgcctcg ccgggctcca acccgtgccg cgccacctgc tcaagatcga
     4681 cccgcagccg gcgaccgtcg tcgagcacca cgagacgcca ctcgccgccg gctttcagag
     4741 ggcccggccc ggtctgcccg cgcgacgctc ccggggggcg gatgtccgtg atcaggggca
     4801 tccgcggccc gtcatcggga ggaccgagca ggcatcccct tggtcaccgg cgcgcccgag
     4861 cgcgcgtccc gcgggagggg accggtctcc ccaccggccg gggtcgcccg cgcctcggac
     4921 ggggcctgcg gggccggccg tgcgatcccc agtttctccc gggtgcgggc ttcgatctcc
     4981 cgagccagct cggggttgtt ctccaggaac tcccgggcgt tgtcgcggcc ctggccgagc
     5041 cgcatgtcct tataggcaaa ccacgtgccc gtccgggtca cgaagttctg ggccacggcc
     5101 agatcgagca ggctccccac cttgctgatg ccgcgcggga agatgatgtc ggcctcggcg
     5161 tcgcggaagg gggcagccag cttgttcttg acgaccctga tccggacccg ctggccgatc
     5221 acctggtcgc ccgatttgat cgactcggtg cggcggatct ccatccgaac cgacgcgtag
     5281 aacttcaacg cgcggccgcc ggtcgtcgtt tcggggttcc caaacattat ccctatcttc
     5341 tcgcggagtt gattgatgaa gacgaccgtg gtgcgggact tgctgatcgt ccccaccagc
     5401 ttgcgcaggg cctgggacat cagccgggcc tgcaggccga cgtgggcgtc gcccatctcc
     5461 ccctcgagct ccgcgcgggg caccagcgcg gcgaccgagt cgacgacgat cacatcgatc
     5521 gcgccggagc ggaccagcat ctcgccgatc tccagggcct gctccccaga gtccggctgg
     5581 gagatcagca ggttgtccac gtccacgccg acgttgcgtg cgtactccgt gttgagcgcg
     5641 tgctcggcat cgatgaacgc ggccactccg ccctcgcgtt gggcctccgc gatgatgtgg
     5701 tacccgaggg tggtcttccc cgaggcctcc gggccgtaga tctcgacgac gcggccgcga
     5761 ggaacgcccc ccacccccag ggccacgtcc agcgcaatca ccccggtggg gatgacatca
     5821 acgctcagct tggcctggtt ctcgccaagc ttcatgatcg accccttccc gaactgccgt
     5881 tcgatctggg tcagggccag atccagggcg cgctgccgct cgttcatctc aggctcctcc
     5941 tacgcatcgc caggccgtgt gtgccgcgca caactatacc aaacatatgt tcgttttgtc
     6001 aatcgcccgc gccactcacg ggacttcacg gagccagacc gtatcgacgg gacgataggt
     6061 ggcccccgat gggtggagcg tactctccat gacgagcaac gcatcgacgg tctgggtccc
     6121 caggatcatc ccgtcgaggt cgtgcaaggg gccggacagg tcggggacgg gcccggcccg
     6181 ccgcacccgg gccaccgtca ggtgcggctg aaacggacgg tcctccaggg gaaacttcaa
     6241 ccggtgaagc gccgtgtcaa gatcccgggc cagcgctatg agccggtcgg caccctcgcc
     6301 gacaccgacc cacaccacct ggggcctccg gggcgagggg aacgccccca ggccggccag
     6361 catgatctcg aaccgcttcg tcctttccgc caccccccgc gcggcagcgg cgaccctgcc
     6421 c
//
LOCUS       DASQGH010000436         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55580, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000436 DASQGH010000000
VERSION     DASQGH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55580"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /gene="cysS"
                     /locus_tag="VEW91_08555"
     CDS             complement(<1..1094)
                     /gene="cysS"
                     /locus_tag="VEW91_08555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYM91667.1"
                     /translation="MRVYSTLSRRIEEFAPLEAGRVRMYVCGPNLYGPSHVGHALSYL
                     VFDVIRRYLEYRGYQVQHIQNFTDIEDRIIETARSQGTTVEALAEQYAARFLREMRAL
                     NVRPADRYPRATDAIPKMLEIVQTLIARGFAYAVDGDVFFRVTAFPAYGRLSGRSLDE
                     MQAGSRVEIDPRKEHPMDFVLWKAAKPGEPSWDSPWGPGRPGWHIECSAMSLSHLGNH
                     LDIHGGGQDVIFPHHENEIAQSEAYTGEPPFVRYWVHNGLLRPGEGQEKMTRHLGNIV
                     TIEEALERYHPDAIRVFVLSSHYRSPATWTEAALEAAMRGAERLRTASDHADAVLRQV
                     PPQRTAGQAAAPGPAGGASGLPQAAAAARAA"
     gene            complement(1115..1465)
                     /locus_tag="VEW91_08560"
     CDS             complement(1115..1465)
                     /locus_tag="VEW91_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91668.1"
                     /translation="MTPQPRSRARGRGRVQGPHLILIERRALREPVVVESEPSGRRPR
                     PTGFWRGTVFCRIVRVLERRWERGESYLRVLADRGCFDLRRVSEMDPWTWKTAGRWEL
                     VAELAAVPVWRPLF"
     gene            complement(1462..2670)
                     /gene="ispD"
                     /locus_tag="VEW91_08565"
     CDS             complement(1462..2670)
                     /gene="ispD"
                     /locus_tag="VEW91_08565"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HYM91669.1"
                     /translation="MIPGVRAAAVVPAAGRGERLGGPTPKSLVLLGGRPLVQYALTTL
                     QGVAEIEAVAVAVPPDATTAIRDLARSAGLTKVVAVVPGGADRQASVAAGLRALPSGP
                     DLVLVHDGARPFLSRRLASEVIAAAARDGSATASLPVGETIKRGEGGWVRDTLDRTAL
                     YRIQTPQAFRRPLLERAHETAEREGIRGTDDAALVERLGTPVRLVPGDPGNFKVTVPE
                     DLALAETVLWGRHDAPRAPRVGVGFDCHRFAPGRALVLGGVAISHPRGLLGYSDADVI
                     AHAVMDALLGAAGCGDIGQHFPPTDPVYRGANSMTLLEQVRDLIANRGWRPAHVDVVV
                     MAEAPLLAPHVPAMRAAMAEVLRLPVESVNVKATTTEGMGAVGREEGIAAHAVASLTP
                     LAGLGEGGAG"
     gene            complement(2667..3761)
                     /locus_tag="VEW91_08570"
     CDS             complement(2667..3761)
                     /locus_tag="VEW91_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYM91670.1"
                     /translation="MTRLIRAIGLIFGSVIGYQVADVVAREILRGAAPQGARLGAPAL
                     GIGLGALIGWTAAPWLQTRFVSAMAWILHRLGSVPLRDVLAAVGGLILGLVTAFLVSI
                     PLLRVPIIGPYVIPLTAVLVFGYLGLHLGIQRREDFLAAFPRLAERLGGRDRRLRRSS
                     VPKLLDTSVIIDGRVADITQSGFLEGPLLVPRSVLDELQRIADASDQIRRNRGRRGLD
                     ILNKMQKDLHAVQIYDEGDELASGQVDAQLVRLARQLGAWIVTNDYNLNKIAELQGVR
                     VLNVNELANAIKPVMLPGEELSVHVIKDGKEAGQGVGYLDDGTMIVVEGGRKHIGETL
                     NTVVTSVLQTVAGRMIFARPKGLERNGAPR"
BASE COUNT          630 a         1452 c         1416 g          591 t
ORIGIN      
        1 gcggcccggg cggccgcggc ggcctggggg aggccggagg ctcccccggc cggcccggga
       61 gccgcggcct gccccgccgt ccgttggggc ggcacctgcc ggaggacggc gtctgcgtgg
      121 tccgatgcgg tgcgcagccg ctccgcgccc cgcatcgccg cctcgagcgc cgcctcggtc
      181 caggtggcgg ggctccggta gtgcgaggag agcacgaaca cccggatcgc gtcggggtga
      241 tagcgctcga gggcctcctc gatggtcacg atgtttccga ggtggcgggt catcttctcc
      301 tgcccctcgc cggggcgcag gagcccgttg tggacccagt accggacgaa cggcggctcg
      361 ccggtgtagg cttcggactg ggcgatctcg ttctcgtgat gcgggaaaat gacatcctgc
      421 ccgccgccgt ggatgtcgag gtggttgccc aggtgggaga gcgacatcgc cgagcactcg
      481 atgtgccacc ccgggcggcc ggggccccag gggctgtccc acgagggctc cccgggcttg
      541 gccgccttcc acaggacgaa gtccatcggg tgttctttcc ggggatcgat ctcgacccgg
      601 gatccggcct gcatctcgtc gagcgatcga cccgagagcc ggccgtaggc gggaaaagcg
      661 gtgacgcgga agaacacgtc cccgtccacc gcataggcga acccgcgggc gatcagggtc
      721 tggacgatct ccagcatctt ggggatcgcg tctgtcgcac gggggtagcg gtcggccggc
      781 cggacgttca gggcacgcat ctcccgcaga aaccgggcgg catactgctc ggccagcgct
      841 tcgacggtgg tgccctggga tcgggcggtc tcgatgatcc ggtcttcgat atcggtgaag
      901 ttctggatgt gctggacctg ataccctcga tactcgaggt accgtcgaat gacgtcgaag
      961 accagatacg acagggcgtg ccccacgtgc gacgggccgt agaggttggg gccgcagacg
     1021 tacatgcgga cccggccggc ctccagcggc gcgaactctt ctatgcgacg gctgagcgtg
     1081 ctgtagacgc gcagggccat cggtcgacgc agggctagaa cagcggccgc caaaccggca
     1141 cggcggcgag ttcggcgacg agctcccacc ggcccgctgt cttccacgtc cacggatcca
     1201 tctcggacac ccggcggagg tcgaaacacc cccggtcggc gagcacgcgg aggtacgact
     1261 cgccgcgttc ccaccgccgt tcgaggacgc gcacgatgcg gcaaaagacc gtcccccgcc
     1321 agaaccccgt cggtctgggc cgcctccccg agggctcgct ctcgaccacg accggctcgc
     1381 gcagggcccg gcgctcgatc aagataaggt gcgggccctg gacgcggccc cttccgcggg
     1441 cgcgggaccg cggctggggc gtcatccggc cccgccttcg cccaagcccg caagcggcgt
     1501 caggctggcc accgcgtggg cggcaatccc ctcctcgcgc ccgacggctc ccatcccctc
     1561 cgtggtcgtg gctttcacgt tgacgctctc gaccgggagg cggagcacct ccgccatcgc
     1621 cgcccgcatc gcgggcacgt ggggcgcgag gaggggggct tccgccatca cgaccacatc
     1681 gacgtgggcg gggcgccacc cgcggttcgc gatgaggtcg cggacctgct ccagcagcgt
     1741 catgctgttc gcgccccgat agaccgggtc ggtcggggga aagtgctggc cgatgtctcc
     1801 gcagccggcc gccccgagca gggcatccat caccgcatgc gcgatcacat ccgcgtccga
     1861 gtacccgagc aatccgcgcg ggtgcgagat ggccacgccc ccgagcacca gcgcgcggcc
     1921 gggggcaaac cggtggcagt caaaccccac gcccacccgg ggagcgcgcg gggcgtcgtg
     1981 ccgcccccag agcaccgtct cagccagcgc caggtcctcc ggaacggtca ccttgaagtt
     2041 gcccggatcg cccggaacca gccgcacagg ggtgcccagc cgttccacga gggccgcgtc
     2101 gtcggtgccg cggatccctt cccgctccgc cgtctcgtgg gcccgctcga ggagtggtcg
     2161 ccggaacgcc tggggcgtct ggatccggta gagtgcggtc cggtcgaggg tgtcgcggac
     2221 ccacccgccc tcgccccgct tgatcgtctc accgaccggc agggaggccg tggccgagcc
     2281 gtcgcgggcg gcggcagcga tgacctcgga ggccagccgt cgggagagga acgggcgggc
     2341 gccatcgtgc acgagcacga gatccggacc cgaagggagc gccctgaggc cggccgcgac
     2401 cgaggcctgc cggtccgcgc cccccgggac cacggccacc accttggtga gcccggcgct
     2461 gcgcgccaga tccctgatgg ccgtggtcgc atccggaggg accgccaccg ccacggcctc
     2521 gatctccgct acgccttgca gggtggtgag ggcgtactgc acgagcggcc ggccccccag
     2581 cagcaccagc gacttggggg tgggcccccc gagccgctcg ccgcgccccg ccgccggcac
     2641 gacggccgct gcccgaaccc ctgggatcac cggggagcgc cgttcctttc gagccccttg
     2701 gggcgggcaa agatcatccg ccctgccacg gtctgcagga cgctggtcac cacggtgttg
     2761 agggtctcgc cgatgtgctt tcggccgccc tcgacgacga tcatcgtgcc gtcgtccagg
     2821 tagccgacgc cctggcccgc ttccttccca tctttgatga cgtgcaccga gagttcttcg
     2881 ccggggagca tcaccggctt gatcgcgttg gccaactcgt tgacgttgag cacgcgcact
     2941 ccctgcagct cggcgatctt gttcaggttg tagtcgttcg tgacgatcca ggctccgagc
     3001 tggcgcgcga gccggacgag ttgggcgtcg acctgcccgg aggcgagctc gtccccttca
     3061 tcgtagatct ggacggcgtg caggtctttc tgcatcttgt tgaggatgtc gagcccccgc
     3121 cgaccccggt tccgacgaat ctgatcgctc gcgtcggcga tccgctgaag ctcatccagc
     3181 accgacctgg gcacgaggag cggcccctcc aggaatccgc tctgcgtgat gtcggcgacc
     3241 cggccgtcga tgatgacgct ggtgtccagg agtttgggca cgctgctgcg ccggagccgg
     3301 cggtcgcgtc ccccgagccg ctcggcgagg cgcgggaagg ccgccaggaa atcctcccgg
     3361 cgctggatgc cgaggtggag ccccaggtat ccgaacacca ggaccgccgt cagcgggatg
     3421 acgtacgggc cgatgatggg gacccggagg agcgggatgc tgaccagaaa ggcggtgacc
     3481 aatcccagga tgagcccgcc gacggcggcg aggacatcgc gcagggggac gctgccgagg
     3541 cggtgcaata tccacgccat cgcgctgaca aaccgggttt gcagccaggg ggccgccgtc
     3601 catccgatca gcgcgccgag cccaatgccg agcgcgggcg caccgaggcg cgcgccctgg
     3661 ggcgcggcgc cgcggaggat ctcccgggcc acgacgtcag cgacctgata cccgatgacg
     3721 gacccgaaga tgagaccgat cgcccggatg agacgagtca tccccactca ccgatgttcg
     3781 acagcgcaaa caacctgcgg cgaagttgcc ggcgattcac tgatggctca ttataccatg
     3841 ccgattgcct tgacacccgc gcacctccgt ctctataatg ggtcgggctt aacggaacag
     3901 ctgctcgagg cgttctcgaa acagcgatca ggcccgggag ggtaccgtcc catgaaagga
     3961 accaagccca cgtttcaaga gcaagtcgac gcctacctaa tccgccaccg cagcatcctg
     4021 gatgtcctga gcaagctgga tgagtctacc gcccgagtca accgcgccat cgcaaaggcc
     4081 gtcaccacg
//
LOCUS       DASQGH010000437         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41521, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000437 DASQGH010000000
VERSION     DASQGH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41521"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..2113)
                     /gene="fusA"
                     /locus_tag="VEW91_08575"
     CDS             complement(32..2113)
                     /gene="fusA"
                     /locus_tag="VEW91_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYM91671.1"
                     /translation="MSERAPLSAIRNIGIVAHIDAGKTTTTERILFYTGRTHRIGEVD
                     EGSATMDWMVQERERGITITSAATTCQWRQHTINIIDTPGHVDFTAEVERSLRVLDGA
                     VVILSAVEGVQPQSETVWRQADRYGVPRVVFINKMDRTGADFFRTLAMMRDRLEARAV
                     PVALPIGVEDGFEGIVDLVRMKSISYTDDLGTRSDVTEIPDALRPQAGEYREQLLEAA
                     AELDDAVAEKYLEGTPVGEDEIRAALRKGTIAAKIVPVLCGSSFRNKGVQPLLDAVVD
                     YLPSPLDIPPVEGQNPKRDTTERRAPDPAGPFCALAFKIVTDSYVGKLTYFRVYSGTL
                     RAGSYVYNATRGAKERVSRILQMHANHREDIPEVLAGNVVAAVGLRETTTGDTLCDEA
                     HPIILETIRFPEPVISVAVEPKTRADEEKLGAALAKLAEEDPTFKVRFDSETAQTIIS
                     GMGELHLEIITDRLLREFKVEANVGRPQVAYRETIRGPAEAEGRYIRQTGGRGQYGHV
                     FLEVEPRPRGAGVEFTNKIKGAAIPKEFIPAVAAGVREAADGGVLAGYPIVDVQVTLT
                     DGSFHEVDSSEMAFKIAGSMAFKAAAEKGGPVLLEPVMKVEAVTPEPYMGDVIGDLHA
                     RRGRISAMEQRGALRIITAEVPLAEMFGYATALRSNTQGRATYTMEFAHYEEVPKNIA
                     ETLVTRAGVSG"
     gene            complement(2180..2650)
                     /gene="rpsG"
                     /locus_tag="VEW91_08580"
     CDS             complement(2180..2650)
                     /gene="rpsG"
                     /locus_tag="VEW91_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HYM91672.1"
                     /translation="MPRKGAVARRATIPDPVFANQTVSRLINKVMTRGKKSTAEHIVY
                     YALDQVKEKSGKEPVKVLEQALHNVMPVLEVRPRRVGGATYQVPVEVRPDRRVSLGMR
                     WLVQYARQRAGKTMRDKLAAEILEASNNAGAAVKRREDTHKMAEANKAFAHYRW"
     gene            complement(2655..3035)
                     /gene="rpsL"
                     /locus_tag="VEW91_08585"
     CDS             complement(2655..3035)
                     /gene="rpsL"
                     /locus_tag="VEW91_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HYM91673.1"
                     /translation="MPTVSQLVRLGRTAETSKSKSPALQMSPQRRGVCIQVKTTTPKK
                     PNSALRKIARVRLTNGMEVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGIRYHIVRGTL
                     DAAGVADRKRGRSKYGAKRPKPAG"
     gene            complement(3276..3548)
                     /locus_tag="VEW91_08590"
     CDS             complement(3276..3548)
                     /locus_tag="VEW91_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HYM91674.1"
                     /translation="MDIERLRAAPQRVIGTNQTTKAIDRGRARVVFVARDADQRVTEP
                     IVKAARDRGVEVVEVESMTVLGRACGIAVGAAAAAILDSTEGVRGQ"
     gene            complement(3712..4134)
                     /locus_tag="VEW91_08595"
     CDS             complement(3712..4134)
                     /locus_tag="VEW91_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYM91675.1"
                     /translation="MSTAVASAVYWDSSAVLSALFRDRHSDEASKRARESGVHLLSTL
                     AWAEVYAVVSRIERERALAKVLVAAARESLEAGPWRRLNAVPDWKLVRDLSSKWPLRG
                     ADLWHLAVAKSLQAELPELAFLSFDVRLTAAAKAEGLA"
     gene            complement(4131..4556)
                     /locus_tag="VEW91_08600"
     CDS             complement(4131..4556)
                     /locus_tag="VEW91_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYM91676.1"
                     /translation="MTPVKCLPSTAGTRELHPIGPIGDLISCDYIDYNMNVVLETTYE
                     RTDVKTRRTGVREAKARLSKLLRDVQRGQEWVITERGTPIARLAPISGRRLSLAERLR
                     RLEDAGLVEPLHREVRNLPQPLPLKEGLAQKWLQESRNS"
BASE COUNT          728 a         1724 c         1509 g          890 t
ORIGIN      
        1 cctgcctccc cgcgatgcct cgagtctacg cctacccgct cacgcccgcc cgtgtgacca
       61 gggtctccgc gatattcttc ggcacctctt cgtaatgcgc gaactccatg gtgtacgtcg
      121 cccgcccctg cgtgttcgac cggagggcgg tcgcatagcc gaacatctcg gccagcggga
      181 cttccgcggt gatgatccgg agggcgcccc gctgctccat cgccgagatg cggccccggc
      241 gcgcgtggag gtcgccgatg acatcgccca tgtacggctc gggggtcacg gcctccacct
      301 tcatcaccgg ctcgagcagg accggaccgc ccttctccgc ggcggctttg aacgccatcg
      361 agccggcgat cttgaacgcc atctccgagg aatcgacctc gtggaaggac ccgtcggtca
      421 gcgtcacctg cacgtccacg atggggtagc cggccagcac ccccccgtcg gcggcctcgc
      481 ggacgcccgc ggccacggcg gggatgaatt ctttgggaat cgccgcgcct ttgatcttgt
      541 tcgtgaactc gacccccgcc ccgcgcgggc gcggctccac ctccaggaag acgtgcccgt
      601 actggccccg tcccccggtc tgccgaatgt accggccttc cgcctcggcc ggcccccgga
      661 tcgtttcccg gtaggccacc tgggggcggc ccacgttggc ctccaccttg aactcccgca
      721 ggaggcggtc ggtgatgatc tcgaggtgca gctcgcccat cccggagatg atcgtctgcg
      781 cggtctcgct gtcgaaccgg accttgaacg tggggtcctc ttccgccagc ttggccagcg
      841 cggcgccgag cttttcctcg tccgcccgcg tcttgggctc gaccgcgacc gagatcacgg
      901 gctcggggaa ccggatcgtc tcgaggatga tggggtgggc ttcgtcgcag agcgtgtcgc
      961 cggtcgtggt ctcccggagc cccaccgccg cgaccacgtt ccccgccaac acctccggga
     1021 tgtcctcgcg gtggttggcg tgcatctgga gaatccggct gacccgctcc ttggcgcccc
     1081 gggtggcatt gtagacgtac gaccccgcgc gcagcgtgcc cgaatagacg cggaagtagg
     1141 tcagcttccc cacgtaggag tcggtgacga tcttgaacgc gagcgcgcag aaggggccgg
     1201 ccgggtcggg ggcgcgccgc tcggtggtgt cgcgcttcgg gttctggccc tccaccggcg
     1261 ggatgtcgag cggggagggg aggtagtcga ccaccgcgtc gaggaggggc tggacgccct
     1321 tgttgcggaa ggaactgccg cacaggaccg ggacgatctt cgccgcgatc gttcccttgc
     1381 ggagcgcggc ccggatctcg tcctccccca cgggcgtccc ctcgaggtat ttctccgcca
     1441 ccgcatcgtc gagttcggct gccgcctcga ggagttgctc gcgatactcc ccggcctggg
     1501 ggcgcagggc gtccgggatc tcggtgacat cgctccgggt gcccaggtca tccgtgtagc
     1561 tgatcgactt catgcggacg aggtcgacga tcccttcaaa gccgtcttcg accccgatcg
     1621 ggagcgccac cgggacggcc cgcgcctcca gccggtcccg catcatcgcg agggtccgga
     1681 agaagtcggc gccggtccgg tccatcttat taatgaagac gacgcggggc accccgtagc
     1741 ggtcggcctg ccgccagacg gtctccgact ggggctggac cccttcgacg gcgctcagga
     1801 tgaccaccgc gccgtcgagc acccggagcg accgctccac ctcggcggtg aagtccacat
     1861 gcccgggggt gtcgatgatg ttgatcgtat gctggcgcca ctggcaggtc gtcgcggccg
     1921 aggtgatcgt gatcccccgc tcccgctcct ggaccatcca gtccatcgtc gccgacccct
     1981 cgtccacttc cccgatgcgg tgcgtgcgcc cggtgtaaaa caggatgcgc tcggtcgtcg
     2041 tcgtcttgcc cgcatcgatg tgggcgacga tcccgatgtt tcgaatcgcc gagagggggg
     2101 cgcgctcgct catcgccgac tgcttcgtct ccagctgcgt tgccacggat cctccaccga
     2161 atgcatgatg gtccctgcgc taccagcggt agtgggcgaa ggccttgttg gcctccgcca
     2221 tcttgtgcgt gtcctcgcgg cgcttcaccg cggcgccggc gttgttgctc gcctccagga
     2281 tctccgcggc cagcttgtcc cgcatcgtct ttccggcgcg ctggcgcgcg tactgcacga
     2341 gccaccgcat gccgagcgag acccggcggt ccggccgcac ctcgaccggc acctgatagg
     2401 tggcgccgcc cacgcggcgg ggccggacct cgaggaccgg catgacgttg tggagcgcct
     2461 gctccagcac cttgaccggc tccttgccgc tcttttcctt gacctggtcg agcgcgtagt
     2521 acacgatgtg ctccgccgtg ctcttcttgc cgcgggtcat caccttgttg atcagccggc
     2581 tgaccgtctg gttcgcgaac accgggtccg ggatcgttgc ccggcgcgcc accgcgccct
     2641 tcctcggcat cgggctaccc tgccggcttg gggcgcttgg ccccgtactt cgaccggccg
     2701 cgcttgcggt cggccacccc ggccgcatcc agggtccccc ggacgatgtg gtaccggatc
     2761 ccggggagat ccttcacccg gcccccgcgg atcaggacca ccgagtgttc ctgcaggttg
     2821 tgcccgatcc cgggaatgta ggcggtgacc tccatgccgt tcgtcagccg cacccgggcg
     2881 atcttccgca gcgccgagtt cggctttttc ggggtcgtcg tcttcacctg aatgcagacc
     2941 ccccgccgct gcgggctcat ctgcagcgcc ggtgatttgc tcttggacgt ctcagccgtc
     3001 cgccccaggc ggaccagctg gctgaccgtc ggcacggtgt ccctccctcc tggatcattc
     3061 ccgcgcgcac acaacacccc cgggcgcggc gtcttccccg cgctgggggg ggatgacgat
     3121 gccggcgacc ggagcggcac actgcgcgcc gcctgacccg cacctgccgg gccatccggt
     3181 cgggttatcg cgtgctaggc tgtatgtggt ttggcatgcg ccatacgcgg tgccgaagcc
     3241 ggcaccctgc ggcgcattat aagggatgcc cccgtctact gcccccgcac accttcagtc
     3301 gaatcgagaa tcgccgccgc cgcggctccc accgcgatcc cgcacgcgcg ccccaggacc
     3361 gtcatcgact ccacctcgac gacctcgacc ccgcggtccc gcgcggcttt cacgatcggc
     3421 tcggtgaccc gctggtcggc gtcccgggcg acaaagacga cccgcgcgcg gcctcgatcg
     3481 atcgccttgg tcgtctggtt cgtcccgatg acccgctgtg gcgcggcacg aaggcgttcg
     3541 atatccacgg tgggcgcctg cgaactctga cccaaccaag taattatact ggccgcccta
     3601 gttcatgtca acaagcgccg cggcccgtgt aaatggtctg agaatccgtc acccctgccg
     3661 agaaagggac tccgcaattt ccccatggcc gcgagcagca cgcgtcgtcg ctcatgccag
     3721 cccttcggct ttggccgccg ccgtcagccg gacgtcaaag gataggaagg ccagttctgg
     3781 cagctcggcc tgaaggcttt tggccaccgc caagtgccac agatcggcac cgcgtagcgg
     3841 ccacttggat gataaatccc gcacgagttt ccagtcaggc accgcgttca agcgccgcca
     3901 ggggcctgct tcaagagact cacgcgccgc agcaacgaga accttggcga gggcgcgctc
     3961 acgctctatt cgcgagacca ccgcatacac ctcagcccaa gcgagagtgg aaagaagatg
     4021 cacccccgat tcgcgagcgc gcttactcgc ctcatcgctg tgccggtcgc gaaagagcgc
     4081 ggacaggacc gcagagctgt cccaataaac agcgctcgca accgcggtgc tcacgaatta
     4141 cggctctcct gcaaccattt ctgtgccagg ccttctttga gggggagagg ttgtggaaga
     4201 ttgcgcacct cgcgatgcaa tggctccacc agccctgcat cttccagccg gcggagacgt
     4261 tccgccaacg atagtctacg cccggaaatc ggcgcgagcc tggcaatagg agttccccgt
     4321 tctgtgataa cccactcttg cccgcgctgg acatcgcgaa gcaacttgct cagccgcgcc
     4381 ttcgcctccc gcaccccggt ccttctcgtc ttcacgtcgg tcctctcata agtagtctct
     4441 aaaactacat tcatattata gtctatgtaa tcacatgaaa tcaagtcgcc gatgggccct
     4501 atcgggtgca gttcccgggt acccgcggtg gacggcaagc atttgaccgg tgtcatctat
     4561 ctccgcagca tcctcgatgc atgcttgacg gcttcagcga atctataact cgctttccgg
     4621 ctttagtggg ggtatagggc gagccctccg gtgttcgtcc ctcccttccg gccatgaatg
     4681 cggggcacgt cgtttggtgc gcgatggacc gctcaatgtg gatgacgcag gccggaatcc
     4741 caactaagcc ttgacattca tcaagggcag cacgtacctc attggcgcgg atacttcgtg
     4801 gccccgaaca gaccgggaca ggtggattgc agaactcggc gtctacggaa g
//
LOCUS       DASQGH010000438         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7702, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000438 DASQGH010000000
VERSION     DASQGH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7702"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1125
                     /locus_tag="VEW91_08605"
     CDS             100..1125
                     /locus_tag="VEW91_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYM91677.1"
                     /translation="MLRLPSFTYCAPQTLEEAIRQIADAGSEGMLVAGGTDLYPNMKR
                     RQFEPKVLVGLRGIRDLNRISGDRHRGMSVGAGVTLAALAAHREVAEGYRALALAAGV
                     VSTPPLRNMGTVGGNLCLDTRCNYYNQTYHWRKSIGFCMKKDGEICLVAPGSPRCWAI
                     SSTDTAPVAIALGAQVRLVGPKGERRIPAAHLYRDDGMQYLAKAPEEILTDIALPPAD
                     GWRSTYWKLRRRGSFDFPILGVAAALRQAPDGTVEDARIVLGAVAAHPVVSAEAVALL
                     RGQRPTPDLIARAAQLAYQPAKPLDNADLTIAYRKKMARVYVERALRELAGLPFEGAP
                     AGSRPDH"
     gene            1178..1612
                     /locus_tag="VEW91_08610"
     CDS             1178..1612
                     /locus_tag="VEW91_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYM91678.1"
                     /translation="MKEIPAGYRTTLEVLVTPDMTVQFGELGPGYPVYATYQLARHFE
                     EAGQKLLLPFLEPGEAGIGFGVSVEHTASALPGMAVKITAVFDRMEGRRLYATMEAFN
                     ALGDPIGTGGTIQVVLPKARIEQDLESLRQRWQRAQANHHGT"
     gene            1656..>2314
                     /locus_tag="VEW91_08615"
     CDS             1656..>2314
                     /locus_tag="VEW91_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM91679.1"
                     /translation="MSATQAARAAGEALTPNDLLQMLYYMRLQRAVEDRGVKLYYQGR
                     IPGAYFTGWGHEAIAVGATYALEPQDLLAPMHRDLAAHVMRGIPLRRIFAQFLDREGG
                     LTRGRDGNVHMGDPRIGVFPHISHMAASVPVAAGMALACRQRSEARVVLTFFGDGATS
                     TGAWHEGVNFAAVLRLAVVLVLENNQYAYSTPVERQTAARAFVDKASGYGIAGVAVDG
                     N"
BASE COUNT          360 a          804 c          816 g          334 t
ORIGIN      
        1 gggaggcgcc ggaggcgcct cgtccccgct gcacaccggg accgatctcg cggacgcgcg
       61 gccgcgcggg gagcgcgcgc aggcgccgga ggatccctga tgctgcggct gccctcgttc
      121 acgtactgcg ccccgcagac cctggaggag gcgatccggc agatcgccga cgcgggcagc
      181 gaggggatgc ttgtcgccgg gggcacggac ctctacccga acatgaagcg gcgccagttc
      241 gagccgaagg tgctcgtcgg gctgcggggg atccgcgacc tcaaccggat ctccggggac
      301 cggcaccggg gcatgagcgt gggggccggg gttacgctcg ccgcgctcgc cgcccaccgg
      361 gaggtcgcgg agggctaccg tgcgctcgcg ctcgcggccg gggtggtcag cacccccccg
      421 ctcagaaaca tggggacggt tggaggcaac ctgtgcctcg acacccggtg caactactac
      481 aaccagacct accactggcg gaagtcgatc gggttctgca tgaagaagga cggggagatc
      541 tgcctcgtcg ctcccggaag cccccggtgc tgggcgatct cgtctacgga caccgctccg
      601 gtagcgatcg ccctcggcgc tcaggtccgc ctcgtgggtc cgaaagggga gcgccgcatc
      661 cccgccgccc acctgtaccg cgacgacggc atgcagtacc tggcgaaggc ccccgaggag
      721 atcctgacgg atatcgccct cccgccggcc gatgggtggc ggtcgaccta ctggaagctc
      781 cgccgccgcg gttcgtttga ttttcctatt ctcggcgtgg ccgccgcgct gcggcaggcg
      841 ccggacggca ccgtggagga cgcgcggatc gtcctcgggg ccgtcgcggc ccatcccgtc
      901 gtctccgctg aggcggtcgc gctcctcaga ggacaacggc cgacccccga cttgatcgcc
      961 cgtgccgcgc aactggcgta ccagccggcc aagcccctcg acaacgccga tctcacgatc
     1021 gcgtaccgga agaagatggc gcgggtctac gtcgagcggg ccctgcggga gctcgccggc
     1081 ctgccgttcg agggtgcgcc ggccggctcc cggccggatc actaaggaca gcgacggtcc
     1141 gtcggctcgc acccgaccgc gtggctgagg tgccaagatg aaggagatcc ccgcggggta
     1201 tcgcaccacg ctggaggtcc tggtgacacc cgacatgacc gtgcagttcg gtgagttggg
     1261 gccggggtat ccggtgtatg ccacctacca actggcccgg cattttgaag aagccgggca
     1321 aaagttgttg ctgccattcc tcgagccggg agaggccggc atcggattcg gggtctccgt
     1381 ggagcacacc gcctccgccc tgccgggcat ggcggtgaag atcaccgccg tctttgaccg
     1441 tatggaaggg cggcggctct acgccaccat ggaggcgttc aatgcgcttg gagaccccat
     1501 cgggacgggc ggcacgatcc aggtcgtgct gcccaaagcg cgcatcgagc aagacttgga
     1561 gagcttgcgg cagcgctggc agcgggccca ggccaaccat cacgggacgt gaaggaatgc
     1621 tgtcgggaga gatccccggg gcagggaggg acgtggtgag cgcgacccag gccgcgcggg
     1681 ccgccggtga ggccctcacg ccgaacgacc tgctccagat gctctactac atgcggctgc
     1741 agcgggccgt cgaggaccgc ggcgtcaagc tgtactacca ggggcgcatc cccggagcct
     1801 acttcaccgg atgggggcac gaggccatcg ccgtcggcgc cacctacgcg ctcgagccgc
     1861 aggatctcct cgcccccatg caccgcgatc tcgccgcgca cgtcatgcgc ggcatcccgc
     1921 tgcgccgcat tttcgcgcag ttcctcgacc gggaaggggg cttgacccga ggccgggatg
     1981 ggaacgtcca catgggcgac ccgcgaatcg gggtctttcc acacatcagc cacatggcgg
     2041 catccgtgcc cgtcgccgcg ggcatggcac tggcgtgccg gcagcggagc gaggcccggg
     2101 tcgtcctgac gttcttcggc gacggcgcca ccagcaccgg cgcgtggcac gagggcgtca
     2161 actttgccgc cgtgctccgc ctcgccgtgg tcctcgtcct cgagaacaac cagtacgctt
     2221 actccactcc tgtggagcgg cagaccgccg cgcgcgcgtt tgtggacaaa gcgtccggat
     2281 acggcatcgc gggcgtcgcg gtggacggca acga
//
LOCUS       DASQGH010000439         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35870, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000439 DASQGH010000000
VERSION     DASQGH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35870"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VEW91_08620"
     CDS             <1..605
                     /locus_tag="VEW91_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91680.1"
                     /translation="AIPDGILTWATTGGSILSLTPDGRSILYHAPITTTPASAQLTAT
                     IGNVTQTITLTIVAGPPVGITIDAPATTVAVGGTLAFGSVVTDQFGNAVTGATIAWTS
                     TAGTINQQGVFTAPSSPGLVVITASTAGRASFVVIDVTSGGFEQFTREATSATSLALL
                     VVAVIAIAACVFLFARYRGMRRELDELRKGGQGGGGGGVA"
     gene            complement(1086..1772)
                     /locus_tag="VEW91_08625"
     CDS             complement(1086..1772)
                     /locus_tag="VEW91_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91681.1"
                     /translation="MNVPEILTKDLIKVYRTGKSEVIALRGLDLHIDDGELIAVRGPS
                     GCGKTTFLNILGGIDRPTAGRIEVDGSNLVDFSDGELVQYRLRKTGFVFQFFNLVPTL
                     TAEENVELPMRLASRDGTDRKTRTTELLDLVGLAHRARHRPDELSGGEQQRIAIAVAL
                     ANDPPLLLADEPTGELDTKTGQEVLNLFQQLNRDLKKTIVVVTDDARVAGIASRVLDI
                     QDGKIVVPAA"
     gene            complement(1769..>2196)
                     /locus_tag="VEW91_08630"
     CDS             complement(1769..>2196)
                     /locus_tag="VEW91_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYM91682.1"
                     /translation="LFRAFFGFMELEIAFVVVILTAGLALILYAASLEREVELAAIRA
                     RGASGWQTAGLLVGEASSIMLVALVVGAAVGVLSAYLSTTFVASGAGESLVPRFLVVP
                     AESLLLLAAAPIAMLITSFLVSLRVARMDIGRVLKLRGG"
BASE COUNT          407 a          714 c          684 g          391 t
ORIGIN      
        1 acgccatccc tgatggcatc ctgacgtggg ctacaaccgg tgggtccatt ctgtcgctca
       61 cgccggacgg ccgttcgatt ctgtaccacg cacccatcac gacgacgcca gcatcggcgc
      121 agctcaccgc cacgatcggc aatgtgacgc agacgatcac cctgacgatt gtcgcgggcc
      181 cgccggtggg aatcaccatc gacgcgccgg cgacgaccgt ggccgtggga gggaccttgg
      241 ccttcggctc agtcgtgacg gatcaattcg gcaacgcggt gaccggggcg acgatcgcat
      301 ggacgtctac ggcaggcacg atcaaccagc agggcgtctt caccgcgcca tcgtcacccg
      361 gattggtcgt catcacggcg agcacagccg gtcgcgcgag cttcgtcgtg atcgacgtca
      421 cgtcgggcgg gttcgaacag ttcacccggg aagcgacctc cgcgacctcg ttggccctcc
      481 tcgtcgtcgc cgtgatcgcg atcgcggcgt gcgtgttcct gttcgcccgc taccgcggga
      541 tgcgccgcga gctcgatgag ctgcgcaagg gcggccaggg tggcggcgga ggcggcgtgg
      601 cctgagcgag aatggagggc ctctcggccc tcccccttta tctttctccc cgcgacatcg
      661 ctacgaccat gacctgcgac aagctgcccg gccgccaggc cgcactcacg gttctcctcc
      721 gacaggcggc ggagacggcc tagcgccaga tcatcctctc tccttgatga cgatttgacc
      781 cttgaggccc tcctgcggcc tttcgcgatc aggaaccgga tctcttcgac tcacgcaacg
      841 ctttggcctc gagtctgagt gaaagagccc tgaacggggg cgatacgtcc gtgaagccat
      901 caggggcaat tctaggccgc tgacggatca cttgctcgga tctcgagaaa gaaccctgaa
      961 gttctccgcc gtccagtggg tgggtggagc cgaccgtgtt ggtcgtgcga tcggcccaga
     1021 ccggacgacg acctgggacc tgcgaaacaa ctgagccccc tacgagcaat gctccgcgtg
     1081 agctcctagg ccgcgggtac gacgattttg ccatcttgga tgtcgaggac gcggctcgcg
     1141 atgccggcaa cccgagcgtc gtcggtgaca acaacgatgg tcttcttgag atcccggttc
     1201 aattgctgga acagattgag gacttcctgg ccagtcttcg tgtcgagctc tcctgtgggt
     1261 tcgtccgcca ggagcagggg cggatcgttt gccaacgcga ccgcgatcgc gatccgttgc
     1321 tgttcgcctc cgctcagctc atcgggccgg tggcgggcgc ggtgggccag gccgacgaga
     1381 tcgaggagtt ccgtggtccg agtcttccgg tccgtgccgt ctctggaggc gaggcgcatc
     1441 ggcagctcga cgttctcctc ggcggtgagc gtcgggacga ggttaaagaa ctggaagacg
     1501 aagcccgtct ttcgcaagcg atattggacg agttctccgt cgctaaagtc gacgaggttg
     1561 gatccgtcca cctcgattcg acccgcggtc gggcggtcaa tccccccaag gatattcaag
     1621 aaggtcgtct tgccgcagcc tgaaggcccc cgaaccgcaa tgagttcgcc gtcatcgatg
     1681 tgcagatcga ggccgcgcaa tgcgatgact tccgatttcc ccgttcgata gactttgatc
     1741 aagtccttcg tcaggatttc tggcacgttc atccacctcg tagtttgagg acgcgtccga
     1801 tgtccattcg cgcgactcga agcgagacga ggaaagacgt gatgagcatc gcgatcggcg
     1861 cggcggcaag gagcagaagc gattccgccg ggacgaccaa gaaccgaggc accaaactct
     1921 ccccggcgcc cgaagcgaca aaagtcgtgg aaaggtacgc cgagaggacg cccactgcag
     1981 cgccaaccac cagcgcgacg agcatgatcg acgacgcctc accgaccaac aggccagcgg
     2041 tctgccatcc gctagcgccg cgcgcgcgga ttgccgccaa ctcgacctct ctctcgaggc
     2101 tcgccgcgta taggatgagc gccagtcccg ctgtgaggat cacgaccacg aacgcaatct
     2161 ccagctccat gaatccaaag aaggcccgga acagcg
//
LOCUS       DASQGH010000440         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10339, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000440 DASQGH010000000
VERSION     DASQGH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10339"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VEW91_08635"
     CDS             <1..881
                     /locus_tag="VEW91_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYM91683.1"
                     /translation="QKNWPANYSDRGRGYDFEGGQPAAYLKDGFLWDAAARAGVSYRV
                     YGEFTEFARFPSRETMPSLKGHVAPGYHGYDLAVKDQTRIDEWLAEFREFDRRGTLPQ
                     LTIMRLPNDHTAATRPGYPTPQAMVADNDLALGRLVDAVSHSRYATDTVIFAVEDDAQ
                     NGPDHVDAHRTVALVAGAYVRRGTVDHAAYSTVSLLRTIELILGIPPLTQYDAAAQPM
                     LGAFSDRAERFEYRAASPRQPLSAVNPPTGPGADESLHLPIDEADEIPPAVLNRILWR
                     AIKGESPMPAPMTTRR"
     gene            complement(916..2160)
                     /locus_tag="VEW91_08640"
     CDS             complement(916..2160)
                     /locus_tag="VEW91_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM91684.1"
                     /translation="MTEQALSDGLMHERSPVPGISRNVWWLGVTSFFTDISSEMLYPL
                     VPVFVTAVLGAPAAAVGVIEGIAEGTASFLKLFSGWISDRLGRRRAIVIAGYALSAVA
                     KPLLAAAGPWTVVLLARFTDRVGKGVRTSARDALLADSAPRAGRGRAFGFHRSADTLG
                     AVLGPLLAIPLLAASHDQLRVLFVLSVIPAALGLAALFAVRDRPVPPGPPSAPPAPAP
                     RLAVAALDTPFRRYLAALGIFALGNSSDAFIILRGRQLGMPLVPIILAYAAYNLIYAL
                     SAYPAGVLSDRIGRRPMVVAGFVFFAAVYAGFAVIGAAWQFWTLMAIYGVYMGLTDGL
                     TRALAADLSAPEGRGTALGWVHMVAGAGALLASLIAGALWTEVGPSAPFFYGAAMAAI
                     AAAYLARLRLGSASRPARAAAA"
     gene            complement(2179..>2461)
                     /locus_tag="VEW91_08645"
     CDS             complement(2179..>2461)
                     /locus_tag="VEW91_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYM91685.1"
                     /translation="VRHLLPSVLPSILVYFTLRIGSAILIASALSFLGLGVQPPTPEW
                     GAMLASGRVFLNVAPHLVIAPGVTISLAVLGFNLLGDGVRDALDPRLRN"
BASE COUNT          425 a          869 c          887 g          280 t
ORIGIN      
        1 tccagaagaa ctggccggcc aactactccg accggggccg cggctacgac ttcgaggggg
       61 gccagccggc cgcatatctg aaagacgggt ttctgtggga cgctgcggcg cgggccgggg
      121 tgtcgtaccg cgtgtacgga gagttcaccg agttcgccag gttcccgagc cgggaaacga
      181 tgccttccct caaggggcac gtggcgcccg ggtaccatgg gtacgatctc gcggtgaagg
      241 accagacgcg gatcgacgaa tggctggcgg agtttcggga gttcgaccgc cggggaaccc
      301 tgccgcagct gacgatcatg cggctgccga acgaccacac ggcggcgacc cggccgggct
      361 atccgacgcc gcaggcgatg gtggccgaca acgacctcgc gctcggccgc ctggtggacg
      421 ccgtctcgca ctcgcggtat gccacggaca cggtgatctt cgcggtcgag gacgatgcgc
      481 agaacgggcc ggatcacgtg gacgcccacc ggacggtcgc gctggtggcg ggtgcgtacg
      541 tccggcgggg gaccgtcgac cacgccgctt acagcacggt ctcactgctt cggacgatcg
      601 agttgatcct ggggatccca ccgctgaccc agtatgacgc ggccgcccag ccgatgctgg
      661 gggcgttcag cgaccgggcc gagcggttcg agtaccgggc cgccagcccg cgccagccgc
      721 tctccgccgt gaaccccccg accgggcccg gggccgacga atcgctccac cttccgatcg
      781 acgaagccga cgagatcccg cccgccgtgc tgaaccgcat tctgtggcgg gccatcaaag
      841 gcgagagtcc gatgcccgcg cccatgacga cgcggcggtg aacgccggcg cgggcctgga
      901 ccgcgcgccc ctccgttacg cggccgccgc ccgtgccggc cgcgacgccg acccgagccg
      961 cagccgtgcc aggtacgcgg ccgcgatcgc cgccatggcc gccccgtaga aaaacggggc
     1021 ggagggtccc acctcggtcc acagcgcgcc cgcgatgagg ctggccaata gcgcccccgc
     1081 acccgccacc atgtgcaccc atccgagcgc tgtgccgcgg ccctccggcg ccgacagatc
     1141 cgccgccagc gcccgcgtca gtccgtccgt caggcccatg tagaccccgt agatcgccat
     1201 cagagtccag aactgccagg ccgccccgat cacggcgaac cccgcgtaca cggcggcgaa
     1261 gaagacgaac ccggcgacca ccatggggcg gcgaccgatc cgatccgaga ggaccccggc
     1321 cggatacgcg gagagggcgt agatcaggtt gtacgccgcg tacgcgagga tgatcggcac
     1381 gagcggcatg cccaactgac ggccccggag gatgatgaac gcgtcgctgc tgttcccgag
     1441 cgcgaagatc ccgagggccg ccagataccg ccggaacggc gtgtcgagcg cggccacggc
     1501 gaggcgcggg gcgggtgcgg ggggagcgct cgggggcccc ggagggaccg gccggtcgcg
     1561 aacggcaaag agggcggcca ggcccagagc ggcgggaatg acggacagga cgaagaggac
     1621 gcgcagttga tcatggctgg ctgccaggag gggaatcgcc agcagcggcc ccaggaccgc
     1681 ccccagcgta tccgcgctgc ggtggaatcc gaacgcgcgc ccgcgcccgg cccgcggcgc
     1741 cgagtccgcc aggagcgcgt cgcgggcaga cgtgcggacc cccttcccaa cccggtcggt
     1801 gaaccgggcg aggagtacga ccgtccacgg gccggccgcc gcgaggaggg gcttcgcgac
     1861 ggcggagagc gcgtaccccg cgatcacgat ggcccgccgc cgcccgaggc gatcggagat
     1921 ccatccggaa aagagtttga ggaagctcgc ggtgccttcg gcgatcccct cgatgacccc
     1981 gacggccgcc gctggggccc ccagcaccgc ggtgacaaag acgggcacca gggggtagag
     2041 catctcgctg ctgatatcgg tgaagaaact cgtcaccccc agccaccaga cgttccgcga
     2101 gatgcccggc acgggcgagc gctcgtgcat cagcccatcg gacagcgcct gctcagtcat
     2161 gcgccgggcc cacccccatc agttccggag tcggggatcg agcgcgtctc gcaccccatc
     2221 gcccaagagg ttgaagccga ggaccgcgag cgagatcgtg acgcccggcg cgatcaccag
     2281 gtggggggcg acgttcaaga acacccgccc gctcgccagc atcgcgcccc actcgggggt
     2341 cggcggctgg acgccgagac cgaggaagct gagggcggag gcgatcagga tggccgatcc
     2401 gatgcgcagg gtgaagtaca ccaggatcga ggggagcacg ctggggagga gatgccggac
     2461 c
//
LOCUS       DASQGH010000441         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52811, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000441 DASQGH010000000
VERSION     DASQGH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52811"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VEW91_08650"
     CDS             complement(<1..367)
                     /locus_tag="VEW91_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYM91686.1"
                     /translation="MRRSRPAAGLAELLRGVGEAPPAPFVAAPFQLAALQALEGGDVV
                     VSAPTGSGKTWIAEQEIAKILRLPPAPAADAMQAGATAAGRVWYTAPLKALSNQKFRR
                     FQREYGEDAVGLLTGERRVN"
     gene            460..897
                     /locus_tag="VEW91_08655"
     CDS             460..897
                     /locus_tag="VEW91_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91687.1"
                     /translation="MKETRGLTVEEKRFLAGLIRQVWRGCQGFVTLVMERGPGEAVYA
                     LEELVEWSTSQSKRLRSRSIRFQTVGLATRRIASELLDDVVTFANGIGDLLGNAQQSD
                     LDPDEVEDEALAMIDGFLAWTTMMAQQLGISRNLRPQTLWNER"
     gene            complement(938..>1520)
                     /locus_tag="VEW91_08660"
     CDS             complement(938..>1520)
                     /locus_tag="VEW91_08660"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HYM91688.1"
                     /translation="EFVDAVESFRDAVMLETYSTLGLRTDTDFLIWKVCPELPPIQEM
                     AAALNRTGLGRYLDLSHSLLALTRKSIYTREEPLEAGFRVDPGAGTYLFVYPFVKTRA
                     WYALPLADRQRMMAEHIRVGHEFPDVQLHTAYSFGLDDQEFVVSFTTDHPKRFQTLVM
                     RLREIEASLYTLRDVPIFTCVRRPLGEILRGLG"
BASE COUNT          259 a          515 c          526 g          220 t
ORIGIN      
        1 ggttgacacg gcgctctccg gtgagcaacc cgaccgcgtc ctcgccgtac tcccgctgaa
       61 aacgccgaaa cttctgattg gacagggcct tgagcggggc ggtgtaccag acgcggcctg
      121 ccgcggtcgc accggcctgc atcgcgtcag cggctggggc aggcggaagc cgaaggatct
      181 tggcgatctc ctgctcggca atccaggtct tgccgctccc ggtgggggcg gagaccacga
      241 catcgccccc ttcgagcgcc tggagggcgg ccagctggaa cggcgccgcc acaaacgggg
      301 cggggggggc ttcaccgacc ccgcggagca actccgccag ccccgccgcc ggtcgtgatc
      361 gcctcacggg ccggaggagg tgcccgcccc cccgtcgaac caggggaggc cggcgggatc
      421 gcgccgaatc gagcgcacga ggcacatggg acccatcaga tgaaagagac gagagggctg
      481 accgtcgagg agaagcgctt cctcgcgggg ctcatccggc aggtctggcg cggctgccag
      541 gggttcgtga ccctggtgat ggagcgcggc cctggggaag ccgtgtacgc gctcgaagag
      601 ctcgtggaat ggtcgacatc ccagagcaag cgcctcaggt cccgctcgat ccggttccag
      661 acggtcgggc tggcgacccg gcgcatcgcg tcggagctcc tcgacgacgt cgtgaccttc
      721 gccaacggca tcggggacct gctgggcaac gcccagcagt cggacctcga ccccgacgag
      781 gtggaagacg aggcgctggc gatgatcgac gggttcctgg cgtggactac gatgatggcc
      841 cagcagctag ggatctcccg caacctccgc ccgcagacgc tctggaacga gcgctgacct
      901 ccgcgcgccg ggcacgcccc cctgcgggcg gtcggggtta gccgagcccc ctcaggatct
      961 ccccgagcgg gcgcctgacg cacgtaaaga tcggaacgtc gcgcagcgtg tacaggctgg
     1021 cctcgatctc ccgcagccgc atcaccagcg tctgaaaccg cttcggatgg tccgtggtga
     1081 acgagaccac aaactcctgg tcgtcgagcc cgaaggagta ggcggtgtgg agctggacgt
     1141 ccggaaactc gtgaccgacc cggatgtgct ccgccatcat ccgctgccgg tcggcgagcg
     1201 ggagcgcgta ccacgcccgc gtcttcacga agggatagac gaacaggtag gtgcccgcgc
     1261 ccgggtccac cctgaagccc gcttcgagcg gctcttcccg cgtatagatc gacttcctcg
     1321 tcaaggccag tagcgagtgg ctcaggtcga ggtaccgtcc gaggccggtc cggttgaggg
     1381 cggcggccat ctcctgaatc ggcggaagct ccgggcagac cttccagatc aggaagtcgg
     1441 tgtcggtgcg gagccccagc gtggagtagg tctccagcat gaccgcgtcc ctgaaacttt
     1501 ccaccgcatc cacgaactcg
//
LOCUS       DASQGH010000442         5641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13421, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000442 DASQGH010000000
VERSION     DASQGH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13421"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VEW91_08665"
     CDS             complement(<1..1093)
                     /locus_tag="VEW91_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91689.1"
                     /translation="MLPRVTRARPPMRAAIVLLVVLALAGAMVFSTSAAPKRGGTLRV
                     AEIGEPLTLDTVATTATQTSSMTHPIFEELFAFDANFRIQPMLAESYTVSKDGRTYTF
                     KLRKNVPFHNAKEMTADDVVASLNRWGKVSPRGASVYKNVESVTASDPSTVVMKLKEP
                     FAPLLSFLAYPNGAAAIMPKEIVDATGAGALKQYIGTGPYKFAEWVPDRHVKLARFDN
                     YAARSEPPSGAAGRREALADEIYFYPVSQVATRTAGVQSGDYDIADATPNQDAYSQIK
                     KDPRVVVEPIKPGDFLTFFFNKKQGLMANEKLRQAVLVALDMQPIMKATFGDPALYSL
                     GPSIYPQGTPWYTTAGQELYNVHDPARARQ"
     gene            complement(1111..1404)
                     /locus_tag="VEW91_08670"
     CDS             complement(1111..1404)
                     /locus_tag="VEW91_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HYM91690.1"
                     /translation="MPLYVGLLNWTEQGVKTAKDTVKRAQGFRTSAEKAGCKIREVLW
                     TMGPYDAIALYEAADDITASRLAIALGMQGYVRSLTMRAFTADEMKKLVGGLP"
     gene            1637..3724
                     /locus_tag="VEW91_08675"
     CDS             1637..3724
                     /locus_tag="VEW91_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYM91691.1"
                     /translation="MHYRIGLDIGGTFTDFVLLDDATGAIHLHKVLTTFPDPAEGALD
                     GLAELCRRVDVGLGEMGTLVHGTTLVTNVIIERDGAPTALLTTRGFRDILEMGKEQRY
                     DIYDLFLRFPPPLVPRRWRVELDERISRDGEVLRPLDPAQVRKAVSHLVAQGVASVAV
                     SFLHAYKNPAHEEAALAAVREAFPDLSISLSSDVVPEIREWERTCTTVCNAYVKPLVD
                     RYLRRLETALAKQGFAGQFYLMQSSGGTATCETARRFPIRLLESGPAGGALVTAFFGA
                     RLGRRDLVAFDMGGTTAKICLVRNGRPEIAPMIEAARVHRFKRGSGLPVKAPVVDMME
                     IGAGGGSIARIDALGLMKVGPHSAGASPGPACYGRGGRDATVTDACLVLGYFDPGYFL
                     GGAMPLDRGAAVEALGRLGRAAGLDTVSAAWGVHRIVSENMAAAARVYMIERGQDPRR
                     YAMLAFGGAGPAHASRVARILGLIEVIVPPASGAASALGFLVTPLSFEFVHSLPGELE
                     SLDWGRVDRLYAEMEAQGRAMLAEGGVDPAEVTYERRAEMRLHGQFHDIELPVPGGRL
                     TPQIVPELSARFEAEYRRRYSTYLPGRQVQALNWRVLVTGPEPRVRLEVAPVDPGAPA
                     TDALKGRRSAYFPETEGFVEVPVYDRYRLRPGAALAGPAIVEEREATTVVGPGDVLEV
                     DGHRNLVVSVGGS"
     gene            3721..5535
                     /locus_tag="VEW91_08680"
     CDS             3721..5535
                     /locus_tag="VEW91_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYM91692.1"
                     /translation="MNATFDPVSLEIMWSRLINIAEECWITIWRTAFSLIIGEAQDFG
                     CELFDPDAESIAHSPRSMPVFNLTLPLAVRHLLAAFPRGTLGPGDVLVTNDPWVCAGH
                     LYDLAVVTPVFRRGALVGLVGSIGHCSDIGGTRDSMSAREIYEEGLQIPPLRLYRRGE
                     LNRDLVEMIRANVRLGEMVLGDIQAQVSSNQVGADRLVAFMDEYGLDSLTPHAHVVQE
                     RAERAMREAIRALPGGTYRSEVTFDGLGEPLTLRCAVAVSGDGMRVDWAGAPPQLPRG
                     GINCTYHYTAAHTTYALKSFLTPEIPSNAGCFRPLRVEVPEGTVLNCRRPASVNQRTQ
                     TGWYCGPAVFKALAPILPDRVQAFTGLPMGMGAYGVDAQGRTFNDHLFQGGGQGASGH
                     GDGQSALLYPTSAGNVSVEMFESRTPLVVECKELVPDSGGPGRHRGGLGQRVRIRKLV
                     DDGLPAFVDLHPHGMLVAPPGLFGGRAGARARAYVTDGKLVLESGATISLAELRHPGQ
                     HATIEIAGGSGYGDPRDRPPERVQADLDEGYITPEGATEYGVTVGPGGEAVRRGGAPP
                     RSRRRSAPTRAARRRQAPKGSRVRRAGDRMASDGRGEG"
     gene            5538..>5641
                     /locus_tag="VEW91_08685"
     CDS             5538..>5641
                     /locus_tag="VEW91_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYM91693.1"
                     /translation="MTDLEAIKGRQQQTWASGDFAMIGTAQIIVGELL"
BASE COUNT          804 a         1880 c         2072 g          885 t
ORIGIN      
        1 gctgcctggc gcgggcgggg tcgtggacgt tgtacagctc ctgccccgcg gtcgtgtacc
       61 agggggtgcc ctgcgggtag atgctgggcc cgagcgagta cagcgcggga tccccaaacg
      121 tggccttcat gatcggctgc atgtcgagcg cgacgagcac agcctgccgg agtttctcgt
      181 tggccatcag gccctgcttc ttgttgaaga agaacgtcag aaagtccccc ggcttgatgg
      241 gctcgacgac cacccgggga tctttcttga tctggctgta ggcgtcctga ttgggggtgg
      301 cgtcggcgat gtcgtagtcg ccgctctgga cccccgccgt gcgggtcgcc acctgcgaga
      361 ccggatagaa gtagatctca tcggcgagcg cctcccgcct gccggcggct ccgctcggcg
      421 gttcgcttct cgccgcatag ttgtcgaagc gggcgagctt gacgtgccgg tcgggcaccc
      481 actcggcaaa cttgtagggg ccggtgccga tgtactgctt gagcgctccg gcgccggtcg
      541 cgtccacgat ctccttcggc atgatcgccg ccgccccgtt cgggtacgcc aggaacgaca
      601 ggagcggggc gaacggttcc ttgagcttca tgacgactgt ggacgggtcc gaggccgtga
      661 ccgactcgac gttcttgtag acggacgcgc cgcgagggct caccttgccc caccggttga
      721 gcgaggcgac cacatcgtcc gcggtcatct ccttggcgtt gtggaacggc acgttcttcc
      781 gcagtttgaa cgtgtaggtg cggccgtcct tgctcaccgt gtacgactcg gccagcatgg
      841 gctggatccg aaagttggcg tcaaaagcga acagttcttc gaagatgggg tgcgtcatgc
      901 tggaggtctg cgtcgccgtc gtcgcaaccg tgtccagggt gaggggctcg ccgatctccg
      961 ccacccggag ggtccccccc cgcttcgggg ccgcggaggt cgagaacacc atcgcgcccg
     1021 caagcgccag gacgaccagc aggacgatcg ccgcgcgcat cgggggtctt gcccgcgtca
     1081 ctctcggcaa catgagctct gggcctcctc tcatgggagt ccgccgacga gcttcttcat
     1141 ctcgtcggcc gtgaaggcgc gcatcgtgag cgagcggacg tacccttgca tgcccagcgc
     1201 gatcgcgagc cggctcgcgg tgatgtcgtc ggccgcctcg tagagggcga tcgcgtcgta
     1261 tggccccatc gtccagagca cctcgcggat cttgcagccc gccttctcgg cggaggtccg
     1321 gaagccctgc gcccgcttga ccgtgtcctt cgcggtcttg acgccctgct cggtccagtt
     1381 caagagcccc acgtacagcg gcatggcgct cccctcctct cagggtgccg gactgcggtg
     1441 ccgcgcgcgc tggcccgtac aggggtgcgt gtggataccg ggttggcgat cgacaacggt
     1501 actggggcga tcgatggatg cgtcgcgcgc aagcggatgg ttcggggtcg caaaaggcgt
     1561 tcctgcatcc ggaggggcag ggatgcccga ctccgcggcg gaagccccac cacatcagac
     1621 gtgaccggag gcgcgcatgc actaccggat cgggctggac atcggcggca ccttcacgga
     1681 cttcgtgctg ctcgacgacg ccacgggagc gatccatctg cacaaggtcc tcaccacgtt
     1741 ccccgatccc gccgaggggg cgcttgacgg gctcgccgag ctctgccggc gcgtcgacgt
     1801 gggcctcggg gagatgggga ccctggtgca cggcaccacc ctcgtgacca acgtgatcat
     1861 cgagcgggac ggcgcgccca cagcgctgct caccacccgg ggattccgcg acatcttgga
     1921 gatgggcaag gagcagcgat acgacatcta cgacctcttc ctccggttcc cgccgccgct
     1981 ggtgccgcgg cgctggaggg tggagctcga tgagcggatc agccgtgacg gagaggtcct
     2041 gcggcccctc gaccccgcgc aggtgcgcaa ggcggtgagc cacctggtgg ctcagggggt
     2101 cgcatcggtg gcggtctcct tcctccacgc ctacaagaac ccggcgcacg aagaagcggc
     2161 gctcgcagcg gtccgggagg cgttccccga tctcagcatc tcgctcagca gcgatgtggt
     2221 gccggagatc cgcgagtggg agcggacgtg cacgacggtg tgcaacgcct acgtcaagcc
     2281 cctcgtcgac cgctacctcc ggcggctgga gaccgcgctc gcgaagcagg ggttcgccgg
     2341 gcaattctac ctgatgcagt cttcaggggg gaccgccacg tgcgagaccg cccgccggtt
     2401 cccgatccgc ctcctggaat cgggccccgc cggcggcgcg ctcgtcaccg cctttttcgg
     2461 tgcgcggttg ggccggcggg atctggtggc cttcgatatg gggggaacca ccgcgaagat
     2521 ctgcctcgtg cgcaacggcc ggcccgagat cgcgccgatg atcgaggccg cgcgggtgca
     2581 ccgattcaag cgcgggtcag gtctgccggt gaaggcgcct gtggtggata tgatggagat
     2641 cggcgcgggg ggcggctcga ttgcgcgcat cgacgcgctt gggctgatga aggtcgggcc
     2701 gcacagtgcg ggggcgtccc cgggccctgc ctgctacggc cgcgggggtc gagacgccac
     2761 ggtcaccgac gcgtgcctcg tgctcgggta ttttgatccc gggtacttcc tcgggggcgc
     2821 gatgccgctc gaccgcgggg cggcggtcga ggcgctcggc cggctcggga gggcggccgg
     2881 cctggacacc gtgtcggcgg cgtggggcgt ccaccggatc gtcagcgaaa acatggccgc
     2941 cgcggcccgc gtctacatga tcgagcgggg gcaggacccc cgccgctacg cgatgctggc
     3001 cttcggcggc gcggggccgg cccacgcatc ccgggtcgcc cgcatcctcg ggctgataga
     3061 ggtgatcgtg ccgccggcgt ccggggcggc gtcggccctc gggtttctcg tgacccccct
     3121 cagcttcgag ttcgtgcact cgcttcccgg ggagctcgag tcgctggact ggggacgggt
     3181 cgaccggctg tacgcggaga tggaggcgca ggggcgcgcg atgctggcgg aggggggcgt
     3241 cgatcccgcc gaggtgacct acgagcggcg ggccgagatg cgcctccacg ggcagttcca
     3301 cgatatcgaa ctgccggtgc cgggcgggcg cctcacgccg cagatcgtgc ccgagttgag
     3361 cgcccggttc gaggccgagt accgccggcg ctacagcacc tacctccccg gccggcaggt
     3421 gcaggcgctg aactggcgcg tgctcgtcac cggcccggag ccgcgcgtcc gcctggaggt
     3481 cgcccccgtc gacccgggcg cgcccgcgac cgacgcgctc aaaggccgtc gcagcgcata
     3541 cttccccgag accgaagggt tcgtcgaggt gccggtgtac gaccggtacc ggctgcgccc
     3601 gggggcggcg cttgccggtc cggcgatcgt ggaggagcgg gaggcgacga ccgtcgtcgg
     3661 gcccggcgat gttctcgaag tcgatggcca ccgcaacctg gtcgtctcgg tggggggatc
     3721 atgaacgcca cgttcgatcc ggtgtcgctc gagatcatgt ggagccgctt gatcaacatc
     3781 gcggaggagt gctggatcac gatctggcgc accgcgttca gcctgatcat cggagaggcg
     3841 caggatttcg gctgcgagct gttcgacccc gacgccgaat cgatcgcgca ctccccgcgg
     3901 tcgatgccgg tgttcaacct gaccctcccc ctcgcggtgc gccacctgct ggccgcgttc
     3961 ccccgcggca ccctcgggcc gggcgacgtc ctcgtgacca acgacccgtg ggtctgcgcc
     4021 gggcacctct acgacctggc cgtcgtcacc ccggtgttcc gccggggcgc gctggtcggg
     4081 cttgtgggat ccatcggaca ctgctccgac atcggcggga cgcgcgactc gatgagcgcc
     4141 cgggagatct acgaggaggg gctccagatc ccgccgctca ggctctaccg acgcggcgag
     4201 ctcaaccggg atctggtcga gatgatccgg gccaatgtgc ggttgggcga gatggtgctc
     4261 ggcgatatcc aggcgcaggt gtcgagcaac caggtgggcg ccgaccggct cgtcgcgttc
     4321 atggacgagt acgggctgga ctcgctgacc ccgcacgccc acgtcgtgca ggagcgggcg
     4381 gagcgggcga tgcgggaggc gatccgcgcg ctcccggggg ggacctaccg cagcgaggtg
     4441 acgttcgacg ggctgggcga gccgctcacc ctgcgctgcg cggtcgcggt ctctggcgat
     4501 gggatgaggg tcgactgggc cggcgcgccc ccgcaactgc cccggggagg gatcaactgc
     4561 acctaccact acaccgccgc ccacacgacg tacgcgctca agtcgttcct gacgcccgag
     4621 atcccctcga acgccggctg cttccgcccg ctgcgcgtcg aggtgcccga gggaacggtc
     4681 ctcaactgcc ggcgtcccgc ctccgtgaac cagcggacgc agaccgggtg gtactgcggc
     4741 cccgcggtgt tcaaggcgct ggccccgatc ctgccggacc gcgtccaggc gttcacgggc
     4801 ctcccgatgg ggatgggggc gtatggggtt gacgcccagg ggcgcacgtt caacgaccac
     4861 ctctttcagg gaggcggcca gggggccagc gggcacggcg acggtcagtc ggcgctgctg
     4921 taccccacct cggcgggcaa cgtttcggtg gagatgttcg agagccggac gcccctggtg
     4981 gtggagtgta aggagctcgt ccccgattcg ggggggcccg gccggcaccg gggcgggctg
     5041 ggccagcgcg tgcgcatccg gaaactcgtg gatgacggcc tgcccgcgtt tgtcgacctc
     5101 cacccgcacg ggatgctggt ggcgcccccg gggctgttcg gtggacgggc gggcgcgagg
     5161 gcgcgggcct acgtgaccga tgggaagctc gtgctggaaa gcggggcgac catcagcctg
     5221 gccgagttgc ggcaccccgg ccagcacgcg acgatcgaga tcgcaggtgg gagcgggtac
     5281 ggcgatcccc gtgaccgccc gccggaacgt gtccaggccg acctcgacga aggctacatc
     5341 accccggaag gggcgacgga gtacggggtg acggtagggc ccggggggga agcggtgcgg
     5401 cgcggaggcg ctccaccccg gtcgcggcgg aggtctgcgc cgacgcgcgc cgcacggcgc
     5461 aggcaggcgc ccaagggttc gcgagttcga agagcaggag atcggatggc gagcgatggc
     5521 agaggggagg ggtaggggtg accgatcttg aggcgatcaa gggccggcag cagcagacgt
     5581 gggcgtcggg cgacttcgcg atgatcggca cagcccaaat catcgtcggg gagctcctct
     5641 g
//
LOCUS       DASQGH010000443         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16069, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000443 DASQGH010000000
VERSION     DASQGH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16069"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..288)
                     /locus_tag="VEW91_08690"
     CDS             complement(97..288)
                     /locus_tag="VEW91_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91694.1"
                     /translation="MEPVHIARVRVYQEKRPNRRAFLGDFPEPVRYGVNTGIKAFYKI
                     EPEEELPSTLDHLVGAAAG"
     gene            368..1510
                     /locus_tag="VEW91_08695"
     CDS             368..1510
                     /locus_tag="VEW91_08695"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HYM91695.1"
                     /translation="MGVATRAWKTHGVLGVILLVLASTPAMGAGVDAAQWKTEFSKHA
                     VPLDEIQPGGPPKDGIPAIDHPRFIAPRDAGWLADREPVVLVEHRGVARAYPLEILVW
                     HEIVNDIVAGDPVVITFCPLCHTALAFRRTLDGLVYDFGTTGFLRKSDLVMYDRQTES
                     WWQQAEGVAIVGRLTGRRLEPVPAQLVAWRTFREAYPKGAVLSRETGFRRAYGQNPYV
                     DYDALDNHPFLFRGAVDRRLRAMERVVAVSLGGETAAYAYPDLARVFVAQDTVGGRAI
                     VVFWAPGVSSALGAGTIARGADVGTSGVFVPSAAGRRLGFHAVAPEDGSPGRWFVDDQ
                     TRSRWDILGRAVDGPLSGSRLAPIVHGDYFWFAWAAFQPGTRVWRP"
     gene            complement(1530..2618)
                     /locus_tag="VEW91_08700"
     CDS             complement(1530..2618)
                     /locus_tag="VEW91_08700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYM91696.1"
                     /translation="MAHRAKTTGEPGTKKPRRSAPPGSPARPAPAPAPDKSLVFLSRL
                     ATEFTAVLSLPDLLEHVMRVLREETGFDSCSLAIMDDRNPEFLIVRAASGIRENFLGL
                     ALPRGKGLHGVVMQTGASLIIPDMHADPRVFRREDRIRSGIYAPLSVGRRPIGVLSAH
                     REQVGAFTQLDLDLLTVVARYLTGAIEVARLHEQLKELAATDALTGLANRRSFLTRLL
                     SEIARARRAERCVSIAIIDLDGFKVINDACGHAKGDETLIQVAEAIARNVRTSDLAAR
                     FGGDEFVLLLPETTAGQAGEILGRLSGLKVPLSEPDGPCHTLNFSWGIAMFPDDGSDP
                     ERLLQVADSRLYSMKKRLYEAKGARTGS"
     gene            complement(2734..3516)
                     /locus_tag="VEW91_08705"
     CDS             complement(2734..3516)
                     /locus_tag="VEW91_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYM91697.1"
                     /translation="MEPSEILFETHGGIAYLTVNRPEQRNAMTWAMYQRLVEACEQVD
                     RDEALKVLVIRGSGGRAFISGTDISQFPAFRGNPQAGIEYEERIDRVVGRLEAVAKPT
                     LASIHGYCIGGGLGIAMACDLRLASADARFGIPNIRLANCLSMHNYARLVALIGPARA
                     KEMIYTARQVDASEAHAWGLTNEVVPAEGLAARTQELAEAITAAPPLTLRASKEAVRR
                     VVDRLLPENPGHDLIALCYNSADFQEGVAAFLDKRPPRWTGR"
BASE COUNT          527 a         1228 c         1288 g          569 t
ORIGIN      
        1 atcgtccgct tcaattcggc cttccacgtc ggcttccagg ttgtttgctg cggggattcc
       61 gcgcgcttcc agcgcgccgg cgagggtccc ggtgagtcac cccgccgccg cgccgacgag
      121 gtgatccagc gtgctgggca gttcctcctc gggctcgatc ttgtagaacg ccttaatccc
      181 ggtgttgacc ccgtaccgga ccggctcggg aaagtcgccg aggaaggccc ggcggttggg
      241 ccgcttctcc tggtagaccc gcacccgggc gatatggacc ggctccatcg cgcaccccgc
      301 gctcccattc cggtatcggc ccgatgacgt taccggccga cagcacaagc gtatagtgaa
      361 gtatagcatg ggagtggcaa cgcgcgcgtg gaagacgcat ggagtgctcg gggtgatcct
      421 cctggtcctg gcctcgaccc ccgcgatggg tgccggggtg gatgccgccc agtggaagac
      481 ggagttctcg aagcacgccg tccccctcga cgagatccag cccggcggcc cgcccaagga
      541 cggcatcccg gcgatcgacc atccccggtt catcgccccg cgggatgcgg gctggctcgc
      601 cgaccgcgag cccgtcgtcc tcgtggagca ccgcggggtg gcccgcgcgt atccgctaga
      661 gatccttgtg tggcacgaga tcgtcaacga catcgtcgcc ggggacccgg tggtcatcac
      721 gttctgcccg ctgtgccaca ccgcgctggc gttccggcgg acgctcgacg gcctcgtgta
      781 cgattttggc accacgggct tcctccgcaa gagcgatctt gtgatgtacg atcgccagac
      841 ggagtcgtgg tggcagcagg cggagggcgt ggcgatcgtc ggcagactca cggggcggcg
      901 gctcgagccg gtccccgcgc aactggtcgc gtggcggacg ttccgggagg cgtaccccaa
      961 gggagcggtg ctctcgcgcg agacggggtt ccggcgggcg tacggccaga atccgtacgt
     1021 ggactacgac gccctcgata accacccgtt cctctttcga ggagccgtgg accgccggct
     1081 gcgggcgatg gagcgggtcg tcgcggtgtc gctgggcggc gagaccgcgg cgtacgccta
     1141 ccccgatctc gcccgggtgt ttgtggcgca ggacacggtg ggaggccgcg cgatcgtggt
     1201 cttctgggcg cccggcgtga gctcggccct gggcgcgggg acgatcgccc ggggggccga
     1261 cgtcgggacg agcggcgtct tcgtgccatc ggccgccggc cggcggctcg gcttccacgc
     1321 ggtggcgccg gaggacggct caccggggcg atggtttgtc gatgaccaga cccgcagccg
     1381 gtgggacatc ctggggcggg cggtggacgg accgctcagc ggaagccggc tggctcccat
     1441 cgtccacggg gactacttct ggtttgcctg ggcggcgttc cagcccggga cgcgggtctg
     1501 gcgcccgtga ggccgcagct cggccgcggt caggaacccg tgcgggctcc cttggcttcg
     1561 tacaggcgct tcttcatcga atagagccgg ctgtcggcga cctgcagcag ccgctccgga
     1621 tcgcttccgt cgtcggggaa catcgcgatc ccccagctga aattcagggt gtggcagggg
     1681 ccgtccggct ccgacagcgg gaccttgagc ccgctgagcc ggcccaggat ctccccggcc
     1741 tgccccgccg tcgtctcggg gagcagcagg acgaactcat cccctccgaa acgggccgcg
     1801 aggtccgagg tgcggacgtt ccgcgcgatg gcctccgcca cctggatcag cgtctcatcg
     1861 cccttggcgt ggccgcaggc gtcgttgata accttgaacc catcgaggtc gatgatggcg
     1921 atgctcacgc agcgctccgc gcggcgggcg cgggcgatct ccgagaggag ccgggtgaga
     1981 aaggaccggc ggttggccag gcccgtcagc gcgtcggtgg cggccagctc tttgagttgc
     2041 tcgtggaggc gggcgacctc gatcgccccc gtcaggtacc gagccacgac cgtcagcagg
     2101 tccagatcga gctgggtgaa cgcgccgacc tgttcccggt gggcgctcag cacgccgatc
     2161 ggccggcgac cgacgctcag gggcgcgtag atgcccgacc ggatgcggtc ctcgcgccgg
     2221 aacacgcggg gatcggcgtg catgtccgga atgatgagcg acgcgcccgt ttgcatcacc
     2281 accccgtgga ggcccttgcc ccgcggcagc gccaggccca ggaagttctc gcgaatcccc
     2341 gacgccgcgc ggacgatcag gaactccggg ttgcggtcgt ccatgatggc cagggagcac
     2401 gaatcaaacc ccgtttcctc gcggaggacc cgcatgacgt gttcgaggag gtcgggaagg
     2461 ctgagcacgg cggtgaactc ggtggccagt cgggagagga agaccaggga cttgtcgggg
     2521 gctggcgcgg gggccggacg tgccggcgat cccggcgggg cactccgcct gggtttcttt
     2581 gtgccgggtt ctccagtcgt ctttgcgcgg tgcgccacgg tggtccccca gaagacagca
     2641 taaaaagggg gggcccgatc cccacccccc ccacaccacc ataagaagat gccccaaaat
     2701 ggggcgggac ggccatgaat ccgggggatg gccctagcgc ccggtccacc gcggcgggcg
     2761 cttgtcgaga aacgccgcca ccccctcctg gaaatcggcg ctgttgtagc atagggcaat
     2821 cagatcgtgg ccgggattct ccgggagcag ccggtcgacg acgcgccgca cggcctcttt
     2881 gctcgcgcgc agcgtgagcg ggggggcggc ggtgatggct tcggccaact cctgggtgcg
     2941 ggcggccagc ccctccgccg ggacgacctc gttggtcagc ccccaggcgt gggcctcgct
     3001 cgcgtccacc tggcgggcgg tatagatcat ctccttggcc cgggccggac cgatgagcgc
     3061 gacgagccgg gcatagttgt gcatcgagag gcagttcgcc agccggatat tgggaatgcc
     3121 gaaccgcgcg tccgccgacg cgagccggag atcgcaggcc atggcgatgc ccagcccgcc
     3181 cccgatacaa tacccgtgaa tcgaggcgag ggtgggcttc gcgacggctt cgagccggcc
     3241 caccacgcgg tcgatgcgct cctcgtactc gatccccgcc tgcgggttgc cgcggaacgc
     3301 cgggaactgg ctgatgtcgg tccccgagat gaacgcccgc cccccgctgc cgcggatcac
     3361 caggaccttg agcgcctcgt cgcggtcgac ctgctcacag gcctccacca gccgctggta
     3421 catggcccac gtcatcgcgt tgcgctgctc aggacgattg acggtcagat acgcgattcc
     3481 cccgtgggtc tcgaacagga tctccgatgg ctccatgcgg tcacctccag gtccgctccc
     3541 gtggacttca tccgcgcggg cccatgggcg gccaggaatt cagcgtgcca agagcggcag
     3601 agcagtccgg gg
//
LOCUS       DASQGH010000444         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_24763, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000444 DASQGH010000000
VERSION     DASQGH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24763"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1310
                     /locus_tag="VEW91_08710"
     CDS             195..1310
                     /locus_tag="VEW91_08710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYM91698.1"
                     /translation="MSVKLGLFTMPFHHPSRPYSTILEEDREAIVLADQLDCSEVFVG
                     EHFASWSERIPSPLMFLSTVIDRTQKIRFGTGVINLPQTHPLIVAAETAMFDHLCRGR
                     FIMGIGPGGLASDMEMFNLPQAEVRAQMLLESIDMVLKLWSQDPPYALPGRFWNISLK
                     DSIQPGFKVGWVPRPYQVPHPPIALSIVMPNSLSAKTAGERGWIPISGNFFNSRYLRG
                     HWERYAEGREQVGQRPDPSVWRVARCVLVTETNAEAEEYLADSTNALSYYYSFFRYNF
                     SAVRQALFMIKPDPDMPDEAATVDVIKRAQVLAGSPSRVLDRLVALREEIGPFGTLLM
                     TGHDWDQPRLWRRSMELLAKDVMPRFQRHAEATLPAS"
     gene            1419..2057
                     /locus_tag="VEW91_08715"
     CDS             1419..2057
                     /locus_tag="VEW91_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYM91699.1"
                     /translation="MGTDRATPQQVPPVSPALKALVGSTGRAKLLAHFFLHPGEAFHV
                     RELARLLDESAGNLLRDLRRLEAIRLLQTERVGNQVRYSLDQAHPLYRDLQHMILKTT
                     GVDAVLREALQPVRGIELAMLYGSFAKGTATSRSDLDLMIIGEVTDRALAPVIAKAEK
                     FLGREVSYTRYPREEAREKIKQRSSFVRNVLAGPGVLVLAHRDDELFALARR"
     gene            2026..2457
                     /locus_tag="VEW91_08720"
     CDS             2026..2457
                     /locus_tag="VEW91_08720"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HYM91700.1"
                     /translation="MTNYSRLLEDRRIEREPAARGQVAQQLSAAQRDLETARGLLAGE
                     NEWAYTIAYNAMLLAGRAVMFSEGYRPTTTGGHAAVVEFLRIHLGPSFAEMTQTMDRM
                     RRQRHRITYDAPGTVSPGQIREAVQTAQQFLSQIEKLIGHK"
     gene            complement(2491..>3338)
                     /locus_tag="VEW91_08725"
     CDS             complement(2491..>3338)
                     /locus_tag="VEW91_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HYM91701.1"
                     /translation="TVGVVGCGLMGSGIAEVCARAGRQVIVREVSEDLLRRGIERIEG
                     SMERGVARGKLTGKDRDAARARIRGTLSLGELRVCEIVLEAIVEKMEDKKRLFTELEE
                     ICPIGTVFASNTSSLSITEMASVTKRPERFVGLHFFNPVPVMKPVEMIRGLRTAEETL
                     ARCRAFCEALGKTVIACKDYPGFIVNLLLVPYLLDAVRALELGVASKEDIDAAVHLGL
                     NHPMGPLTLLDFVGIDTTYYIAEAMYAEFKDARYAAPPLMRKMVLAGYHGRKTGRGFY
                     DYSSA"
BASE COUNT          611 a         1089 c         1023 g          615 t
ORIGIN      
        1 ctcggcgtca cccgcgcctc cttttccctg gaccgcgacg gcacgcggct ccctagcgtc
       61 caagctcctg ccaatgggcg tctactgcgg tcccaccggg aaaccctgct cggcgggcag
      121 gaaccgaaac gctcgcccgg aactgcttgg tgatccgccg cttcacttcg cggggaggaa
      181 tcgaggaggt cgtcgtgagc gtcaagcttg gtctgttcac gatgccgttt catcacccca
      241 gccggcccta cagcaccatc ctcgaggaag atcgggaggc cattgtccta gccgaccagc
      301 tggattgctc ggaggtcttt gtgggcgagc atttcgcctc gtggagcgag cgaatcccct
      361 cgcctctcat gttcctctcg actgtgatcg accggacgca gaagattcgc ttcggcacgg
      421 gggtcatcaa tcttccacag acccacccgc tcattgtcgc ggcggagacc gccatgtttg
      481 accacctgtg ccgcgggcgc ttcatcatgg ggatcggccc ggggggcctc gcgagcgata
      541 tggaaatgtt caacctgccc caggccgagg tgcgcgccca gatgctgctc gaatcgatcg
      601 acatggtgct gaagctctgg tcccaggacc caccctatgc acttcccgga cggttctgga
      661 atatttccct caaggacagc atccagccgg gattcaaggt cggctgggtg ccgcggccgt
      721 accaagtgcc gcacccaccg atcgcgctgt cgatcgtgat gccgaattcc ctgagcgcca
      781 agaccgccgg agaacggggg tggatcccga tctccggcaa cttcttcaat tcacggtacc
      841 tgcggggcca ttgggagcgc tatgctgaag gacgcgagca ggtgggacaa cgtcccgatc
      901 cgagcgtatg gcgcgtcgcg cgctgcgtcc tcgtcaccga gacgaacgcg gaggctgagg
      961 agtatcttgc agattcgacc aatgcgcttt cgtattatta cagcttcttc cgatacaatt
     1021 tcagcgcggt gcgtcaggcg ctgttcatga tcaaacctga tcccgacatg cccgatgaag
     1081 ccgcgacggt cgatgtgatc aaacgcgctc aagtcctggc ggggagcccg agccgcgtgc
     1141 tcgatcgatt ggtcgcgctc cgcgaggaga tcggcccctt cgggacgctg ttgatgaccg
     1201 ggcacgattg ggatcaaccg aggctctggc gtcgctcgat ggagctgctg gcaaaggacg
     1261 tcatgccgcg gttccagagg cacgcggagg ccaccctccc agcttcctaa tatcgcctca
     1321 ccgcggccaa ggcggagccc ttcggcgaca aacgcgttca tggctccctc ggagcgccgc
     1381 ttgacttcgt tctcaatttg agtacaattg tactcgaaat gggaacggat cgcgcgacac
     1441 cgcaacaggt cccaccggtc tcaccggcgc tcaaggcgct ggtgggctcg accggccggg
     1501 ccaaactcct cgcgcatttc ttccttcacc ccggcgaggc cttccatgtc cgtgaactag
     1561 ctcgactgct tgatgagtcg gctggaaacc tgctgcgcga tctgcgccgc cttgaggcga
     1621 tccggcttct tcaaacggaa cgggtgggca atcaagtccg ctattctctg gatcaagcgc
     1681 acccgctcta cagggacctg cagcacatga tccttaagac caccggcgta gacgccgtgc
     1741 tccgggaggc gctacagccg gtgcggggca tcgaattggc catgctttac ggctctttcg
     1801 ccaagggaac ggccaccagc cggagcgacc ttgacctcat gatcattggt gaggtaacag
     1861 accgcgccct agcccccgtg attgcgaagg cggagaagtt cttggggcgc gaggtatcct
     1921 acacccggta cccgcgagag gaagcacgcg agaagatcaa acagcgcagc tcctttgtgc
     1981 gcaacgtgct cgcggggcca ggcgtcctgg tgctcgctca ccgcgatgac gaactattcg
     2041 cgcttgctcg aagatagaag aatcgagcgg gaaccagcgg cccgcgggca ggtggcccag
     2101 caactatccg ctgcccagcg ggatctcgag acggcccgcg ggttgctcgc cggtgagaat
     2161 gagtgggcct ataccattgc atacaacgcc atgctgttgg caggacgagc ggtcatgttc
     2221 tccgagggat accggccgac gaccacaggc ggccacgccg ccgtggtaga gttcctgcgt
     2281 atccacctgg gtccatcatt cgccgagatg acccagacta tggaccgaat gcgccgacag
     2341 cgacatcgga tcacctacga tgcacccggc accgtgtcgc ccggccaaat tcgagaggct
     2401 gttcagaccg cgcagcaatt cctttcacag atcgagaaat tgatcggcca caagtgaaac
     2461 ggggaagatt gtagagcgtt cctagcaagc tcaggcgctg gagtagtcgt agaatcccct
     2521 cccggtcttc cggccgtggt atccggccag gaccatcttg cgcatgaggg gcggggcggc
     2581 gtaccgggcg tccttgaact ccgcgtacat cgcctccgcg atgtagtagg tcgtatcgat
     2641 gccgacgaag tccaggagcg tcagggggcc catcggatgg ttgagcccca aatgcaccgc
     2701 ggcgtcgatg tcctccttgc tcgccacccc cagttcgagc gcccgcactg catcgaggag
     2761 gtagggaacg aggaggaggt tgacgatgaa tcccgggtag tccttacagg cgatgacggt
     2821 cttgccgagc gcttcgcaga aggcgcggca tcgggccagg gtctcctcgg cggtgcgcag
     2881 tccccggatc atctcgaccg gcttcatgac cgggaccggg ttgaagaagt ggagccccac
     2941 gaaccgctcc gggcgcttgg tgacggacgc catctcggtg atcgacagcg agctggtgtt
     3001 gctggcgaag acggtcccga tggggcaaat ctcctcgagc tcggtgaaga gccgcttctt
     3061 gtcctccatc ttctcgacga tcgcctcgag cacgatctcg cacacccgga gttccccgag
     3121 cgagagcgtt cctcgaatgc gggcccgggc tgcgtcgcgg tccttgccgg tcagcttgcc
     3181 gcgcgccact ccccgctcca tcgacccctc gatccgctcg atcccccggc gcaggagatc
     3241 ctcgctgacc tcgcgcacga tcacttgtct gcctgcgcgc gcgcacacct cggcgatgcc
     3301 cgaccccatg agcccgcacc cgacgacccc gaccgttc
//
LOCUS       DASQGH010000445         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55635, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000445 DASQGH010000000
VERSION     DASQGH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55635"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VEW91_08730"
     CDS             complement(<1..501)
                     /locus_tag="VEW91_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM91702.1"
                     /translation="MRTRGRLGALGLALLVAVALVPVRAGAQGAIPLQYYYPVGVSGP
                     LNKLMTEMVSDFNGGHPGIRVEPVFAGNYVQAMAKAMTAVMGGTPPDLAVFDTPELFS
                     LLDRNAIIPLDDFIAKSGGKPWIDDYYQTLLLNARANGHVYSIPWRRSTPIFYYNKDL
                     FRKAGLD"
     gene            560..1360
                     /gene="ubiE"
                     /locus_tag="VEW91_08735"
     CDS             560..1360
                     /gene="ubiE"
                     /locus_tag="VEW91_08735"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HYM91703.1"
                     /translation="MTRNVCLPTGPPGERVGRGQVSEDEKARYIRRMFGAIAPRYDLA
                     NALISGGLHRKWKAATARLAQVPPGGRAVDVCCGTGDLALLLARSVGPRGRVVGIDIS
                     EEMLGVARRKAAAARLGRVVEFALGDAEALGLPDAAFDGATVGFGIRNTIHPETALRE
                     LHRVLRPGAHLAVLEFSTPQSGVVQRLYGWYSYTVIPWMGRLASRHTDAYLYLSTSIR
                     AWPDQAAFAGMMARAGFAPVRYQNLLGGIAAIHVGVRPAGDRVAEGLA"
     gene            1357..1635
                     /locus_tag="VEW91_08740"
     CDS             1357..1635
                     /locus_tag="VEW91_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HYM91704.1"
                     /translation="MSEREKTGVFQVGARQLRDVEEALRYVYDALRERGYNPVDQIVG
                     YLVSGDPTYITSHKDARTLIRQIDRDRLLEELVRCYVRVRLGGAADSR"
     gene            1595..2914
                     /gene="murA"
                     /locus_tag="VEW91_08745"
     CDS             1595..2914
                     /gene="murA"
                     /locus_tag="VEW91_08745"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYM91705.1"
                     /translation="MSGCAWGEPPILADAIATDRGTEKLLITGGVRLRGRLRVAGAKN
                     SSLAVIAAAALAPDRSVLENVPMCRDVLTILEILRALGVRAEFTSPGRLELDARGLND
                     HVAPYELCRQMRASIYVAGMLLARLGRAQVPLPGGCVIGSRPVDFHIRGFEALGAQVV
                     TQHGFLNAYGPRLTPASYYVPRSSVGTTINLMLAASRTPGATVLQNVAREPEVVDTAV
                     FLSLMGARVRGAGTSTITVEGTPHLRGATYTIIPDRIEAGTYLIAAAATRGDVLIEDL
                     ISEHLTALLAKLVEAGVAVESEPTGIRVRADADLRPVDVDTAPYPGFPTDLHPQLAAM
                     LAVVAGRSVVRETIFESRFGYVDELRRMGADIRVEGDTVIITGVPYLSGAPVEAMDIR
                     AGAALVIAGLEGRGETQITHLESIDRGYDGLKEKLHALGARIDRIRA"
     gene            2940..>3143
                     /locus_tag="VEW91_08750"
     CDS             2940..>3143
                     /locus_tag="VEW91_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM91706.1"
                     /translation="MVRCTFYGAARTVTGSMHLVEGGGVRLLLDCGLFQGLKELRLRN
                     WRDFPFDPKQLDAVVISHAHIDHT"
BASE COUNT          448 a         1083 c         1131 g          481 t
ORIGIN      
        1 atccagcccc gccttccgga agaggtcctt gttgtagtag aagatcggcg tgctgcgccg
       61 ccaggggatg ctgtagacgt gcccgttggc ccgcgcgttg aggaggaggg tctggtagta
      121 gtcgtcgatc cacggcttcc ccccgctctt cgcgatgaag tcgtccaggg ggatgatcgc
      181 gttccggtcg agtagggaga agagctccgg cgtgtcgaag accgccaggt ccgggggcgt
      241 ccctcccatc accgcggtca tcgccttcgc catcgcctgc acgtagttgc ccgcgaacac
      301 cggttcgacc cggatccccg gatggccgcc gttgaagtcg ctgaccatct cggtcatcag
      361 cttgttgagc ggccccgaga cgccgacggg atagtagtac tgcagcggaa tcgctccctg
      421 cgcgccggcc cggaccggca ccagggcgac tgccacgagc agcgccagtc ccagcgcccc
      481 caggcgccca cgagtccgca tcttgcaccc ccttcgcgaa tcacaggaaa ggttctttgt
      541 ccgcgcgaac cctacctccg tgacccggaa cgtctgcctc ccgaccgggc cgcccggtga
      601 gcgcgtgggg cggggccagg tctcggagga cgagaaggcc agatacatcc gccggatgtt
      661 cggggcgatc gctccccggt acgacctcgc caacgcgctg atcagcgggg gcctccaccg
      721 gaagtggaaa gcggcgacgg cgcgactggc gcaggtaccc cccggcggcc gggcggtgga
      781 cgtctgctgc gggaccgggg atctggcgct cctcctggcg cgatcggtcg ggccccgcgg
      841 gcgggtggtg gggatcgaca tcagcgagga gatgctcggg gtggcgcgcc ggaaggcggc
      901 cgccgcccgg cttgggcgcg tcgtggagtt tgcgctgggc gacgccgaag cgctggggct
      961 cccggacgcc gcgttcgacg gggccacggt agggttcggg atccggaata ccatccatcc
     1021 cgagacggcg ctccgcgagc tccaccgggt gctgcgtccc ggcgcccatc tggccgtcct
     1081 ggagttcagc acgccgcaaa gcggtgtcgt ccaacggctc tatgggtggt attcatatac
     1141 agtgattccc tggatgggac ggctggcctc gcgccacacc gacgcgtacc tctacctgtc
     1201 gacgtccatc cgggcgtggc ccgaccaggc agcgttcgcc gggatgatgg cccgggcggg
     1261 gtttgcgccg gtgcgctacc agaacctgct cggggggatc gcggccatcc atgtcggcgt
     1321 ccggcctgcg ggcgaccggg tggcggaggg gctggcatga gcgagcgcga gaagacgggt
     1381 gtgttccagg tgggcgcccg gcagctgcgg gatgtggagg aggcgcttcg gtacgtgtat
     1441 gacgcgctcc gggagcgggg gtacaatccc gtggaccaga tcgtcgggta tctcgtctcg
     1501 ggcgatccga cctacatcac cagccacaag gatgcccgga ccttgatccg gcagatcgac
     1561 cgcgaccggt tgctcgaaga gctcgtgcgg tgctatgtcc gggtgcgcct ggggggagcc
     1621 gccgattctc gctgacgcga tcgccactga ccgggggacg gagaagctgc tcatcacggg
     1681 cggcgtgcgg ctgcgcggcc ggcttcgggt cgccggggcc aagaacagct cgctggccgt
     1741 gatcgccgcc gcggccctcg ccccggaccg ctcggtgctc gaaaacgtcc cgatgtgccg
     1801 cgatgtgctg accattctgg agatcctgcg ggcgctcggc gtccgcgccg agttcaccag
     1861 cccgggacgg ctcgagttgg acgcccgggg cctcaacgac cacgtggccc cgtacgagct
     1921 ctgccggcag atgcgggcgt cgatctacgt cgccgggatg ctcctcgccc ggctgggccg
     1981 cgcccaggtc ccgctcccgg gggggtgtgt gatcgggagc cggccggtcg acttccatat
     2041 ccggggattc gaggcgctgg gcgcgcaggt ggtgacccag cacgggtttc tcaacgcgta
     2101 cgggccgcgc ctgacccccg ccagctacta cgtgccccgg agcagcgtgg ggaccaccat
     2161 caacctgatg ctggcggcct cgcggacccc gggtgccacg gtcctgcaaa acgtcgcgcg
     2221 cgagccggag gtcgtcgaca ccgcggtctt cctctcgctg atgggcgcgc gcgtgcgggg
     2281 cgcgggcacg agcaccatca cggtcgaggg aaccccccac ctgcggggtg cgacctatac
     2341 gatcatcccc gatcggatcg aggcggggac gtacctgatc gccgcggcgg cgacccgcgg
     2401 ggatgtgctg atcgaggatc tgatctcgga gcacctcacc gccctcctgg cgaagctcgt
     2461 ggaggcgggg gtcgcggtgg aatccgagcc gaccggcatc cgggtgcggg ccgatgccga
     2521 cctccggccg gtcgatgtgg acaccgcccc ctacccgggg ttccccaccg acctgcaccc
     2581 gcaactcgcg gcgatgctcg ccgtggtggc cgggcggagc gtggtccggg agaccatctt
     2641 cgagagccgg ttcggctatg tggacgagct gcgccgcatg ggcgcagaca tccgggtcga
     2701 gggggatacg gtgatcatca ccggcgtccc ctacctctcc ggggcgccgg tcgaagccat
     2761 ggacatccgc gccggcgcgg cgctggtcat cgcgggcctc gagggccggg gcgagaccca
     2821 gatcacccac ctcgagagca tcgaccgggg gtacgacggc ctcaaggaga aactccacgc
     2881 cctcggcgcc cggatcgacc ggatccgggc ctgaggcccc gcccgcttcc tcccgcccca
     2941 tggttcgctg cacgttctac ggcgcggccc ggacggtaac gggctcgatg cacctcgtcg
     3001 agggcggcgg cgtgcggctg ctgctcgact gcggcctgtt ccaggggctg aaggagctgc
     3061 gcctgcggaa ctggcgcgac ttccccttcg acccgaagca gctcgacgcc gtcgtcattt
     3121 cccacgccca catcgaccac acc
//
LOCUS       DASQGH010000446         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44431, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000446 DASQGH010000000
VERSION     DASQGH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44431"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /gene="polX"
                     /locus_tag="VEW91_08755"
     CDS             <1..1631
                     /gene="polX"
                     /locus_tag="VEW91_08755"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HYM91707.1"
                     /translation="FLAEDVNELYRSGGRKALDAIPGVGSSIAEKIEELVTTGGLEYY
                     ERIKRKIPTVTLELTKIPNVGPKTALKLYETLKVKDIAELEQVIDTPKAAKYFKKKTR
                     DRIKMGIALLRRLSGRIILPYAEPIARGFVEALRHTPGVAQVDPVGSLRRMRETVGDI
                     DIVCAARDPQQAIDRFISHPGVKQILARGDTKATIIHADGVQVDLEILPQAQYGSLLQ
                     HFTGSKEHNIALRTWAVEHGFSVSEHGIKKGDALHTFEHEKDVYAFLGMDWIPPELRE
                     NRGEIEAALQHRLPDLVEEEDIKGDLQSHSTWSDGHDRIEMLARAAIDRGYEYLAITD
                     HTKGLGVTHGLDAARVAERREEIVAARRKYGPRIHLLEGLEVDIRADGHLDLPDEVLR
                     AMDIVVASIHSAFAQSKQQITRRLVGAIHNPHVDIIGHPSGRLRGEREAYPVDWDEVF
                     TAAARYGVALEINAFPNRLDLNDTLAREAHRRGVRLVISTDAHKAEHLAMMRYGIAVA
                     RRAWLTRADVLNTRPWTEFSAGLSRREGSESTER"
     gene            complement(1655..2095)
                     /locus_tag="VEW91_08760"
     CDS             complement(1655..2095)
                     /locus_tag="VEW91_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYM91708.1"
                     /translation="MWRLLIVIAILGLSVAGAPVMPTSLDASEMLQTAWRWSTPPPEI
                     FRPVPDRQGLRLRMIDFAFRPSVLQAAQGVPIVLELINSGATPHILLIPVLGVRETLL
                     PGERQIVYLGRAEAGIYEFFCAIPEHRDAGMIGRLIVGPGIVAR"
     gene            complement(2304..>2623)
                     /locus_tag="VEW91_08765"
     CDS             complement(2304..>2623)
                     /locus_tag="VEW91_08765"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792907.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HYM91709.1"
                     /translation="GFPFIFRGALDVRARAITEQMKIAASRALAALAREDVPDVVLRA
                     YGLGALRFGRGYLIPTPLDPRVPLWVAPAVAEAAMLTGAATETVDLTAYRAGLVRRLS
                     HPA"
BASE COUNT          505 a          881 c          855 g          382 t
ORIGIN      
        1 agttcctggc cgaggacgtc aacgagctct accggtccgg gggccgcaag gccctcgacg
       61 ccatcccggg ggtgggctcc tcgatcgccg agaagatcga ggaactcgtc acgaccgggg
      121 ggctcgagta ctacgagcgg atcaagagga agatcccgac ggtgacgctg gaactcacca
      181 agatcccgaa cgtggggcca aagaccgcac tcaagctcta cgagaccctg aaggtcaagg
      241 atatcgcgga gctcgaacag gtgatcgata cgccgaaggc cgcaaagtac ttcaagaaaa
      301 agacccgcga ccgcatcaag atggggatcg ccctcctccg gaggctctca ggccgcatca
      361 tcctcccgta cgccgagccg atcgctcggg ggttcgtgga ggcgctacgc cacaccccgg
      421 gggtcgccca ggtcgatccg gtaggatcgc tgcgccgaat gcgtgagacg gtcggcgaca
      481 tcgacatcgt ctgcgccgcc cgagatcccc agcaggcgat cgaccgtttc atttcgcatc
      541 cgggcgtcaa gcaaatcctg gcccggggcg ataccaaagc cacgatcatc cacgccgatg
      601 gtgtgcaggt ggatctggag atccttcccc aggcccagta tggctccctc ctccagcatt
      661 tcaccggttc caaggaacac aatatcgcat tgcggacgtg ggccgtggag cacggcttct
      721 ccgtctccga gcacggaatc aagaagggtg acgcgcttca cacgttcgag cacgagaagg
      781 acgtctatgc gtttctgggg atggactgga tcccccccga gttgagggag aaccgggggg
      841 agatcgaggc ggcgctccaa caccgcctgc ccgacctcgt ggaggaggag gacatcaaag
      901 gcgatctcca gagtcactcc acctggtcgg acggacacga ccggatcgag atgctggccc
      961 gcgcggccat cgaccgcggc tacgagtatc tggcgatcac ggaccatacc aaggggctgg
     1021 gcgtcaccca cggactcgac gcggcgcggg ttgctgagcg gcgcgaagag atcgttgcgg
     1081 cacgacggaa atatggcccg cgcattcacc tcctcgaagg gctggaggtc gacatccggg
     1141 ctgacggcca cctggacctg ccggatgaag tcctccgggc gatggacatc gtcgtggcct
     1201 cgatccattc cgcgtttgcc caatcgaagc agcagatcac gaggcggctc gtcggcgcga
     1261 tccacaaccc ccacgtggat atcatcggcc atccttcggg ccggctgcgc ggggagcgcg
     1321 aagcctatcc ggtggattgg gacgaggtgt tcacggcggc cgcacggtac ggggtggcgc
     1381 tcgagatcaa cgccttcccc aaccgcctgg acctcaacga cacgctggcc agagaggccc
     1441 accggcgtgg cgtgcgcctg gtcatcagca cggacgccca caaggccgag cacctcgcca
     1501 tgatgcgtta cggcatcgcc gtggcccgcc gcgcctggct cacgcgggct gacgtgctga
     1561 acacccgccc ctggacggaa ttctccgcgg ggctgtcccg ccgggaggga agcgagtcaa
     1621 ccgagcgctg acggatcgat cgggccgggc acgcctaccg ggccacgatc cccgggccga
     1681 caatcaaccg gccgatcatc cccgcgtcgc gatgctcggg aatggcgcag aagaactcgt
     1741 agatcccggc ctccgctctc ccaaggtaca cgatttgtcg ttcacccggc aacagggtct
     1801 ctcgaactcc caggaccgga atgagcagga tgtggggggt tgccccgctg ttgatcagct
     1861 cgaggacgat cggaacgccc tgcgccgcct gaagcacgct cggccggaac gcgaaatcga
     1921 tcatgcgcaa ccggaggccc tgccgatccg gcaccggacg aaaaatctcc ggcgggggag
     1981 tgctccagcg ccacgccgtc tggagcatct cgctcgcatc caacgaggtc ggcatgaccg
     2041 gggcgccggc cacgcttaat ccgagtatgg cgatcacgat cagcaagcgc cacatacggt
     2101 gcctcccggg cagcccccga ggccgtatgg ggcagctcga gagccactcc cactactatt
     2161 ctccgttgcg ccgggggctc gggcaatcgg gtcgtcgatg gatggtttcg tcgggtcccg
     2221 gccttcctac gatctcccgc atgggagggg cgcagaaaaa gggggggcga gcgtctacgg
     2281 ggcgacggcc gcccacaccg ccgtcacgcg ggatggctga gccggcgaac cagaccggcc
     2341 cgatatgccg taagatcgac ggtctcggtg gccgcgcccg tcaacatcgc cgcctcggcg
     2401 accgcagggg caacccacag cggaacccgg ggatccagcg gcgtggggat caggtagccg
     2461 cgaccgaatc ggagcgcccc caacccgtag gcccttagca caacgtccgg cacatcctcc
     2521 ctggccagcg ccgcgagggc cctcgaggcg gcgatcttca tctgctcggt gatcgcccgc
     2581 gcccggacat ccagcgctcc gcggaagatg aatgggaacc cga
//
LOCUS       DASQGH010000447         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27354, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000447 DASQGH010000000
VERSION     DASQGH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27354"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /gene="rapZ"
                     /locus_tag="VEW91_08770"
     CDS             complement(<1..849)
                     /gene="rapZ"
                     /locus_tag="VEW91_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HYM91710.1"
                     /translation="MARRQPDAGLPTRTISDEIEFLIVTGLSGAGKTLATRTLEDLGF
                     FCVDNLPPALIPKFAEIIQESQGRILRVALVIDIRGGEFFDALGAALADLDALGIRFQ
                     IVFLDASDEMLVRRFEETRRKHPLGGSILDGIRTERRRLQPLKEQAHKIIDTSTLTVR
                     ELKEELADTFLRTDGRRVLSVIITTFGYKYGIPLDADLVFDVRFLPNPYYVEALRAHP
                     GNSPEVREFVLRSPQAVEFQRRLHDMLAYLLPQFTAEGKSHLTVAVGCTGGKHRSVVI
                     GEELAQF"
     gene            complement(849..1184)
                     /locus_tag="VEW91_08775"
     CDS             complement(849..1184)
                     /locus_tag="VEW91_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYM91711.1"
                     /translation="MKFVFRWLINAVALYLVTLVVPGVKVPGFGAALVAALVLGIVNA
                     VIRPVVLILTLPLNILTLGLFTFVVNAGMLYIVSRVTLLRLEGFWAALLGAIVLSIVS
                     AVLTHLVRD"
     gene            complement(1259..2158)
                     /gene="sucD"
                     /locus_tag="VEW91_08780"
     CDS             complement(1259..2158)
                     /gene="sucD"
                     /locus_tag="VEW91_08780"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYM91712.1"
                     /translation="MAILVGRDTRLLVQGITGYQGMFHTELMLGAGTRVVAGVTPGKG
                     GSRIHGVPVFEAVDEAVRETGANASVIFVPARFARDAALESVAHRLDPVVIITEGIPV
                     RDAIEVVAYAARQGVRVLGPNGPGVTTPGQCRVGIMPTHLFTPGHIGVVSRSGTLTYE
                     IVARLTRAGLGQSTAVGLGGDPVVGMSFVDVLQLFNADPETHGIVLIGEIGGRAEEDA
                     AEYIAAHLRKPVAAYIAGRTAPEGKRMGHAGAVISGTEGTAAHKIQALEAAGVAVATL
                     VSGVPALLLERLRAAQAPKGIPG"
     gene            complement(2160..3269)
                     /gene="sucC"
                     /locus_tag="VEW91_08785"
     CDS             complement(2160..3269)
                     /gene="sucC"
                     /locus_tag="VEW91_08785"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HYM91713.1"
                     /translation="MKLHEYQAKKVFARYGLPVEQGVVIERPEQVPRLDLRYPVVVKA
                     QVLVGGRGKAGGIKLAQTPAEAEAHARAILGMEIKGERVARVLVVPAADISAEYYLAF
                     VTDRTLRCAVAVASAAGGIEIEAVARTTPEKIATAAIDPFLGCLPFHARALGRRLGLS
                     GPLLEQFAAIAANLYRLYWTEDAELAEINPLAIAGSVFLCVDAKLVLDDNASYRHPDR
                     PPSEELTSLEREARASGLAYVELDGDIAVIGNGAGLVMSTLDLLAHVGGRAANFLDIG
                     GGATAEGMRKALNIVQQKPGVRAIFINIFGGITRCDDVARGIVQDPPRAPASIRLTGT
                     NEAEGQRILRAAGISAGLDPDEAAREAVALARKAR"
     gene            complement(3288..4226)
                     /gene="mdh"
                     /locus_tag="VEW91_08790"
     CDS             complement(3288..4226)
                     /gene="mdh"
                     /locus_tag="VEW91_08790"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HYM91714.1"
                     /translation="MARTRAKVTIVGAGNVGHSAAQWLLSHRLADLVLVDVIEGMPQG
                     KALDLMEAGPIEGFDARITGTNGYDETADSDVVVIVAGIARKPGMSREDLLATNAGIV
                     RGVVEQVTRRSPHAHLIVVTNPLDVMVYLAYKVSGFPPERVVGQSGALDSTRFRTFIA
                     QALGVSVQDVSAIVIGAHSDTHMVPLASLATVGGIPLHRLLPADRIAALVERTRKGGA
                     EIVNLLRTGSAFFAPGAAIAQMVEAILLDRKRVLPLSAYLTGQYGVRDLYVGVPAVLG
                     AGGLERILEAPMDAGERAAFDAAVASIRENVALLKL"
BASE COUNT          668 a         1554 c         1463 g          642 t
ORIGIN      
        1 gaattgcgcc agctcctccc cgatcaccac cgaacgatgc ttgccccccg tgcacccgac
       61 cgctaccgtc agatggctct tcccctccgc ggtgaactgc gggaggaggt aggcgagcat
      121 gtcgtgcagt cgccgctgga actccaccgc ctgcggggac cgaaggacga actcgcggac
      181 ctcggggctg ttgcccgggt gcgcgcggag ggcctccaca tagtaagggt tgggcaggaa
      241 gcgcacgtcg aagaccaggt cggcgtcgag cggaatcccg tacttgtacc cgaaggtcgt
      301 gatgataacc gagagcaccc ggcgcccatc cgtccggagg aacgtgtcgg caagctcctc
      361 cttgagctcc ctcaccgtca gcgtgctggt gtcgatgatc ttgtgcgcct gttccttgag
      421 cggctgcagg cgccggcgtt cggtgcggat cccgtcgagg atgctgccgc cgagcggatg
      481 cttgcgccgc gtttcctcga agcgccggac cagcatctcg tcggatgcgt ccaggaacac
      541 gatctggaac cggatcccca acgcatccag gtccgccagc gcggccccca gggcgtcgaa
      601 gaactcgccg ccccgaatgt cgatgacgag cgcgacccgg aggatgcgcc cctgcgactc
      661 ttgaatgatc tccgcaaact tcgggatcaa ggcgggggga aggttgtcga cgcagaagaa
      721 ccccaggtcc tccagcgtcc gggtcgcgag ggtcttgccg gccccgctca gccccgtgac
      781 gatgagaaac tctatctcat cggagatcgt gcgtgtgggc aggcccgcgt cgggctgccg
      841 gcgcgccatc agtccctgac caggtgcgtc aggaccgcgc tgacgatgct gagcacgatg
      901 gcccccagga gcgccgccca aaatccctcc agccgcaaca gggtcaccct ggacacgatg
      961 tagagcatgc cggcgttgac gacgaacgtg aacagcccca gcgtgaggat gttcagcggg
     1021 agggtcagga tcagcaccac cggccggatc accgcgttga cgatgcccag caccagcgcg
     1081 gccaccagcg ccgcgccaaa cccgggcacc ttgacgcccg gcaccacgag cgtcacaaga
     1141 tagagggcga ccgcattgat cagccaccgg aagacgaatt tcataagtat tttattatac
     1201 catccggccg gcgcccccag gcggctgcca ccgggcccgg cgcccgggct cgatgccgtc
     1261 acccgggaat gcccttcggg gcctgcgcgg cgcgcagtcg ctccaggagg agcgcgggca
     1321 ccccgctgac cagggtggcc acggccacgc cggcggcttc gagggcctgg atcttgtggg
     1381 cagcggtccc ctcggtccct gagatgaccg ccccggcgtg gcccattcgc ttcccctccg
     1441 gcgcggtgcg gccggcgatg taggcggcaa ccggtttcct caggtgggcg gcgatgtact
     1501 cggccgcatc ctcttcggcc cgccccccga tctcgccgat cagcacgatg ccgtgcgtct
     1561 ccggatcggc gttgaacagc tgcaacacgt cgacaaagct catcccgacc accggatccc
     1621 cgcccagccc cacggccgta gactgaccga ggcccgcacg ggtcagccgg gccacgatct
     1681 cgtacgtgag cgtgccgctg cgcgagacca cgccgatgtg gccgggggtg aagaggtggg
     1741 tgggcatgat ccccacgcgg cactgccccg gcgtggtcac cccggggccg ttggggccga
     1801 gcacacgcac cccctgccgg gccgcgtagg ccaccacctc gatcgcatca cgcaccggta
     1861 tgccctccgt gatgatcacc accggatcga gccggtgggc caccgactcg agcgcggcat
     1921 ccctcgcgaa cctcgccggg acaaagatca cactggcgtt ggccccggtc tcccggacgg
     1981 cctcatcgac cgcctcgaac accggcacgc cgtggatcct agacccgccc ttgccgggcg
     2041 tcaccccggc gacgacgcgt gtcccggccc cgagcatcag ctcggtgtgg aacatcccct
     2101 ggtagccggt gatcccctgc accagcaggc gcgtgtcgcg gccgacgagg atcgccatgc
     2161 tagcgcgcct tccgtgccag ggcgaccgct tcgcgggcgg cctcgtcggg gtcgagccct
     2221 gcggagatcc cggccgcccg gaggatgcgc tgcccctccg cctcattggt gcccgtgagc
     2281 cggatgctcg ccggggcgcg gggcgggtcc tggacgatcc cgcgggcgac atcgtcgcac
     2341 cgggtgatgc cgccgaagat attgatgaag atcgcccgga ccccgggctt ctgctggacg
     2401 atgttcaggg ccttgcgcat cccctcggcc gtcgcccccc cgccgatgtc caggaagttg
     2461 gccgcgcggc cgccgacgtg cgccaggagg tcgagggtgc tcatgacgag ccccgcgccg
     2521 ttgccgatga ccgcgatgtc cccgtcgagt tcgacatagg ccaggccgct cgcccgggct
     2581 tcccgctcga gggaggtgag ctcctccgag ggggggcggt cggggtgccg gtaggaggcg
     2641 ttgtcgtcaa gcacgagctt ggcgtcgacg cagaggaaca cggaccctgc gatcgccagc
     2701 gggttgatct cggcgagctc ggcgtcttcg gtccagtaca gccggtagag gttcgccgcg
     2761 atcgcggcga actgctcgag cagcggcccg gatagcccga ggcggcgccc gagggcgcgg
     2821 gcgtgaaaag gcaaacaccc caggaacgga tcgatcgcgg cggtcgcgat cttctcgggg
     2881 gtggtccggg cgacggcctc gatctcgatc ccgcccgccg cagaggccac cgcgaccgcg
     2941 caccggagcg tgcggtccgt cacgaacgca agatagtatt cggcgctgat gtccgcggcc
     3001 gggacgacca gcacgcgcgc gacccgctcc cctttgatct ccatgccgag gatggcccgg
     3061 gcgtgcgcct ccgcctcggc gggggtctgg gccagcttga tccccccggc cttcccgcgg
     3121 ccgcccacga gcacctgcgc tttgacgacg accgggtagc gtaggtcgag gcggggcacc
     3181 tgctccgggc gctcgatgac gaccccctgc tcgaccggca gcccatagcg ggcaaagacc
     3241 ttcttggcct gatactcgtg cagcttcatc gatccgcgct tcggcggtta gagcttgagc
     3301 agggcgacgt tttcgcggat gctggccacc gcggcgtcga acgctgcccg ctcgccggcg
     3361 tccatcggcg cctccaggat gcgctccaac cccccggcgc cgagcaccgc cggcacgccg
     3421 acatagaggt cgcgcacgcc gtactggccg gtgaggtagg cagacagcgg cagcacccgc
     3481 ttccggtcca gcaggatcgc ctcgaccatc tgcgcgatcg ccgccccggg ggcgaagaag
     3541 gcgctgccgg tccgcaggag gttcacgatc tcggccccac ccttgcgggt gcgctccacg
     3601 agggcggcga tccggtccgc gggcaagagg cgatgcagcg ggatcccccc gaccgtggcg
     3661 aggctggcca gcgggaccat gtgcgtatcg ctgtgggcgc cgatcacgat ggccgagacg
     3721 tcctggaccg agacgccgag ggcctgcgcg atgaacgtgc ggaaccgcgt gctgtcgagc
     3781 gcaccgctct gccccaccac gcgttcggga ggaaacccgc tgaccttgta ggccaggtag
     3841 accatcacgt ccagggggtt ggtcaccaca atcaggtgcg catgcgggga ccgacgggtc
     3901 acctgctcga cgacgccccg gacgatcccg gcgttggtcg ccagcaggtc ctcccggctc
     3961 atgccgggct tgcgggcgat cccggccacg atcacgacga catcggagtc ggcggtctcg
     4021 tcatagccgt tggtgccggt gatacgggca tcgaatccct cgatcggtcc cgcttccatg
     4081 aggtcgagcg ccttgccctg gggcatcccc tcgatgacgt ccaccagcac gaggtccgcg
     4141 agccggtgcg agagcagcca ctgcgcggcc gaatgtccga cgtttccggc tccgacgatc
     4201 gtcaccttgg cgcgtgtcct ggccacccgg acctcccttc gggccgtgct caccgctgca
     4261 tcgcgtcgat cacggcgtcg gcgaactcgc tggtgcgcac ctcgcgggcg tcctcggtca
     4321 gccgcgc
//
LOCUS       DASQGH010000448         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16077, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000448 DASQGH010000000
VERSION     DASQGH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16077"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VEW91_08795"
     CDS             <1..183
                     /locus_tag="VEW91_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HYM91715.1"
                     /translation="KGILIDATYGRRTRAVVITDSGHVVLSAVQPETVAHRFTSKEMV
                     GDEEEEAAAEAAAESE"
     gene            180..722
                     /locus_tag="VEW91_08800"
     CDS             180..722
                     /locus_tag="VEW91_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HYM91716.1"
                     /translation="MIVTVSGEIGAGKSTVAKALARALGLRYISAGEIFREEARRRGL
                     SLGALGQLAEEDPAIDQELDRMQVEAARGGGVLIDSRLSGWLIDGNLRVWLRAPLAVR
                     AARVAARDGLSVEEALRELQVREECERRRYNATYQILLTDLSRYHIIVDTATWSAQEI
                     VEALLPLARRFSPERLGRPS"
     gene            727..1296
                     /locus_tag="VEW91_08805"
     CDS             727..1296
                     /locus_tag="VEW91_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HYM91717.1"
                     /translation="MAQTLAGRVVVVGVCGSIAAYKVAMVVRRLRTAGAEVHVLMTPA
                     AARFVGPPTFRALARQPVITDMWDLHSPWDEPHVGLGERADLYLIAPATADMVGKLAA
                     GLADDLVCATALATRAPLLVAPAMSEPMATSPVVQDNLARLRARGVRVIGPDRGPLAS
                     GMVGLGRMAEPEAIVAEVAALLGRRKRRR"
     gene            1293..>2970
                     /gene="priA"
                     /locus_tag="VEW91_08810"
     CDS             1293..>2970
                     /gene="priA"
                     /locus_tag="VEW91_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HYM91718.1"
                     /translation="MSQDLGACAEVLLDLPARRADRRLTYRIPDPLHSAVRIGSRVLV
                     PLGPRTAPGFVIALAPSAPVLHREIREILAVEDSTPLFAQRMLDLAAWVAEQTVSTLL
                     EAVHCLAPPGIFRRRAAPSTAPGGHAGDRLRAWPLPSPRASAGAAPPESAQAERATLV
                     WGEGAGRRAWIVAAALATAEHGGQVLIVVPEIALVPLLLDPLRAALGERAMAFHSGVP
                     ERERRAAWVRIASGEVPVVVGTRSALFAPLDRLRLIVVDEEHDPAHQADAAPRYHARD
                     VALRRGALEGARVVLGSPTPSMETYAMVTSGRIDCVRLPQAVPPPRVTLVDLRAERTR
                     GRHGLLTPPLMVAIRSHLRSGGRVALFVNRTGYARILRCPECGFSVRCPRCEVTMPYD
                     RESRTISCRICGAAAPAPGACPRCGGVALRGIGPGTARVEEVVRRLFPALAIARIDRE
                     TAPRFAQVASAFASGRIRLVVGTQLLLRAREIRPSLVGVLDADLPLHLPDFRAAERAL
                     QQLRAVVSLAAGVPGAEAIVQTQLPGHPVLSALATGDDEAVYRRELAVRSE"
BASE COUNT          337 a         1067 c         1133 g          433 t
ORIGIN      
        1 aagggtatcc tgatcgacgc gacctacggc cgccggaccc gggccgtggt catcacggac
       61 agcggccacg tcgtcctgtc cgccgtccag ccggagacgg tcgcccaccg gttcaccagc
      121 aaggagatgg tgggggacga ggaggaggaa gccgccgccg aggcggcggc tgaatcagaa
      181 tgatcgtcac cgtgagcggg gagatcggtg ccggcaagag cacggtggcg aaggcgcttg
      241 cgcgcgcgct gggcctccgg tacatctcgg cgggcgagat cttccgcgag gaggcgcgcc
      301 ggcgcggcct gagcttgggg gcgctggggc aactggcgga agaggacccg gcgatcgacc
      361 aggaattgga ccggatgcag gtggaggcgg cccgcggcgg cggggtgctc atcgacagcc
      421 ggctcagcgg gtggttgatc gatgggaatc tgcgcgtgtg gctgcgggcg ccgctcgcgg
      481 tgcgggccgc gcgggtcgcc gcccgggatg ggttgtcggt ggaggaggcc ctgcgcgagt
      541 tgcaggtgcg cgaggagtgc gagcgccgcc ggtacaatgc gacctaccag atccttttga
      601 ccgacctcag ccggtatcac atcatcgtgg acaccgcgac gtggagcgcc caggagatcg
      661 tcgaggcgct gctgccgctg gcccgcaggt tctcgcccga gcgattgggc cgcccctcct
      721 gagccggtgg cccagaccct cgcaggccgg gtggtggtcg ttggcgtctg cggcagcatc
      781 gccgcgtaca aggtcgccat ggtcgtccgt cggctgcgca cggccggcgc ggaggtccat
      841 gtgctcatga ctcccgccgc cgcccggttc gtgggccccc cgaccttccg agcgctggcg
      901 cggcagccgg tcatcacgga catgtgggat ctccacagtc cgtgggacga accgcacgtg
      961 gggctcgggg agcgcgcgga tctctacctc atcgcgccgg ccaccgcgga catggtgggg
     1021 aagctggccg ccgggctcgc cgatgacctc gtgtgcgcga ccgcgctggc cacgcgggcc
     1081 cccctcctgg tcgcccccgc gatgagcgaa cccatggcga cctccccggt cgtccaggac
     1141 aatctggcgc gcctgcgcgc gcggggggtt cgcgtgatcg ggccggaccg tggtcccttg
     1201 gcctccggca tggtcgggct cggccggatg gccgagcccg aggcgatcgt cgccgaggtg
     1261 gcggccctcc tgggccggcg caaacgccgc cggtgagtca ggatctcggg gcgtgcgctg
     1321 aggtcctgct cgatctcccg gcgcgccgcg ccgaccgacg gctgacctac cgaattcccg
     1381 atccgttgca cagcgccgtc cggatcggat cccgcgtcct cgtccccctg gggccgcgca
     1441 cggcacccgg gttcgtgatc gcgctggcgc cctccgcccc cgtcctccac cgggagatcc
     1501 gggagattct cgcggtcgaa gattccactc cgctcttcgc ccagcggatg ctcgatctcg
     1561 ctgcctgggt ggccgagcag acggtgtcga ccctcctcga ggccgtccac tgcctggctc
     1621 cgccggggat ctttcgccgc cgcgcggcac cgtcgactgc gcctgggggg cacgccgggg
     1681 accggttgcg tgcgtggccc ctcccgtccc cgcgcgcgtc tgcgggggcc gcccccccgg
     1741 agtccgctca ggcggagcgg gccaccctgg tgtgggggga gggcgccggg cggcgggcgt
     1801 ggatcgtcgc cgcggccctg gccaccgccg agcacggggg ccaggtcttg atcgtggtcc
     1861 cagagatcgc cctggtcccc ctcctcctcg accccctgcg cgcggcgctt ggggaacggg
     1921 cgatggcgtt tcactcgggg gtgcccgagc gggagcggcg ggccgcgtgg gtccggatcg
     1981 cctccgggga ggtccccgtg gtcgtcggga cgcgctccgc gctgttcgcc cccctggatc
     2041 ggctgcgcct gatcgtggtc gatgaggagc acgatccggc gcaccaggcc gacgccgccc
     2101 cacggtacca cgcgcgggac gtcgcgctgc ggcgcggtgc gctcgaagga gcccgggtgg
     2161 tcctgggatc gcccaccccg tcgatggaga cctacgccat ggtcacctcg ggccgcatcg
     2221 actgcgtgcg cctcccccag gcggtcccgc ccccccgggt cacgctcgtg gacctgcgcg
     2281 cggagcggac gcgggggcgc catgggctcc tgaccccgcc gctcatggtg gccatccgca
     2341 gccatctgag atccgggggg cgggtcgcgc tgttcgtcaa ccggaccggg tatgcgcgca
     2401 tcctccgctg ccccgagtgc ggcttctcgg tgcggtgccc gcgctgcgaa gtgacgatgc
     2461 cctacgaccg ggagagccgc acgatctcct gccggatctg cggcgcggcg gcgccggccc
     2521 cgggggcgtg cccgcggtgc ggtggggtgg cgctgcgcgg gatcggcccg gggaccgcgc
     2581 gggtggagga ggtcgtgcgc cgcctgttcc ccgcgctcgc catcgcgcgc atcgaccgcg
     2641 agaccgcccc gcggttcgcg caggtggcga gcgcatttgc ctcgggccgg atccgcctgg
     2701 tcgtcgggac gcagctgctg ctgcgggccc gcgagatccg ccccagcctc gtcggtgtgc
     2761 tcgacgcgga tctccccctt cacctgccgg atttccgcgc ggcggagcgg gcgctgcagc
     2821 agttgcgcgc ggtggtgtcg ctcgccgcgg gcgtcccggg cgcagaagcg atcgtgcaga
     2881 cccagctgcc cggccatccg gtgctgtccg ccctcgccac cggggatgac gaggcggtgt
     2941 accgtcgcga gctcgcggtg cggagcgaat
//
LOCUS       DASQGH010000449         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_18998, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000449 DASQGH010000000
VERSION     DASQGH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18998"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..517
                     /locus_tag="VEW91_08815"
     CDS             224..517
                     /locus_tag="VEW91_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HYM91719.1"
                     /translation="MGGDEDVVTLLDEDGVEHEFSVVDVLEVDQRRYAILQPMVGGEE
                     AEAAVIFRMEDDTLVTIEDDAEFERVRQAFEAADTEQADDQPVSAEGQSEPSH"
     gene            536..1033
                     /locus_tag="VEW91_08820"
     CDS             536..1033
                     /locus_tag="VEW91_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HYM91720.1"
                     /translation="MLRWLRPAAQVSTVFDIDLIALRTRGIRGIILDLDNTIVPWGAR
                     EASPELVGWISGAHAAGFRLCIVSNNIGGRVTRIAAMLGLPVVTGALKPRRKALRRAL
                     SVMGTTPEETALVGDQLLTDILGGNRLGVHTILVRPQARREFLGTRVARMVERVLLRG
                     GPVNR"
     gene            1042..2124
                     /locus_tag="VEW91_08825"
     CDS             1042..2124
                     /locus_tag="VEW91_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM91721.1"
                     /translation="MSPAATAHLDRARAYLAASHVDALLILKTENRRYVTGFTGSAGL
                     ALVTGMEALLAVDFRYEEQADAEAPGATVIRGGRDPLKALAAAVEGRGLSRIGFEAEF
                     VPYAQVARLREKLAPAELVALSDVDRLRWVKDPAEVAAISRAAEIADAAYLRVLEMLR
                     PGVSERGAAVELETFMRRAGADRVAFDSVLASGPRSALPHGLATERVMGPGEFVTLDF
                     GAMWGGYCSDCTRTVVLGSADEQQRRVYGVVLDAQRRALEMIKPGVGCRDVDAGARSA
                     IADAGYGAAFGHSLGHGVGLEVHEGPRLSPQEEAVLESGMVVTVEPGVYLPGWGGVRI
                     EDTVVVTQDGCRILTRAPKELHVLPV"
     gene            2177..2737
                     /gene="efp"
                     /locus_tag="VEW91_08830"
     CDS             2177..2737
                     /gene="efp"
                     /locus_tag="VEW91_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYM91722.1"
                     /translation="MISSNDFRPGVHVLLDGEVYVIVASEHSKVAMRHGLVKAKIRNT
                     KTGVTTERTFRGGERVPLVHLELKTMQYLYTTGDEYVVMDKTTFEQLPLPRALLGDAV
                     RFLAENTDVTVVFHEDAPIGVELPTAVELRVVETAPGFRGDTVSGGTKPATLQTGAVV
                     QVPLFVTAGEVVRIDTRTGTYLERVR"
     gene            2815..>2934
                     /locus_tag="VEW91_08835"
     CDS             2815..>2934
                     /locus_tag="VEW91_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYM91723.1"
                     /translation="MELEGATTQPVTVSEFQRHPKDSGSPDVQIARLTERINHL"
BASE COUNT          446 a          921 c         1090 g          477 t
ORIGIN      
        1 gcgaggacgg agggcggcaa cgtactcccg gatgctgcgt gggctcatgg tgcctcctcg
       61 gccttggtaa ccgatttatg aggcaaccat agcctttttg gtaaccggaa ttacgagtca
      121 gatcctcgcg ttgacacccc ctgcggccga tgttagactt ttcttgcgtt tgacgcccgc
      181 gtgtggggcg cctggcgagg ttcgcgcaag ggggtgtccc gcggtgggcg gcgatgagga
      241 cgtcgtgacg ttgctcgatg aggacggtgt cgagcacgaa ttcagcgtgg tggacgtcct
      301 cgaggtagat cagcggcggt acgccattct gcagccgatg gtggggggcg aagaggcaga
      361 ggccgcggtg atcttccgga tggaagatga taccctcgtc accatcgagg atgacgcgga
      421 gttcgagcgg gttcggcagg cgttcgaggc ggcggacacg gagcaggccg acgatcaacc
      481 ggtgtcggca gagggccaga gcgagccttc ccactgatcc ccaggcaggt gatgcgtgct
      541 ccggtggctg cggccggccg ctcaggtctc aacggttttt gacatcgatc tgatcgccct
      601 tcgtacccgc gggatccgcg ggatcattct ggatctggac aacaccatcg ttccctgggg
      661 cgctcgcgag gcgagccccg aactcgtcgg gtggatctcc ggcgcccacg ccgcgggctt
      721 tcggctgtgc atcgtgagca acaatatagg cggccgcgtc acgaggatcg cggcgatgct
      781 ggggctgccc gtggtgaccg gggcgctcaa gccccgacgc aaagccctcc gccgcgccct
      841 gtccgtgatg ggcacgacgc cggaggagac cgccctggtc ggggatcaac tgctgaccga
      901 catcctgggg gggaaccggc tcggtgtgca cacgatcctt gtgcgcccgc aggcccgccg
      961 tgagttcctg ggtacccgcg tggcgcggat ggtggagcgc gtcctgctgc gcggcggccc
     1021 ggtgaaccga tgatgctcga ggtgagcccc gccgccaccg cccacctgga ccgggccaga
     1081 gcgtatctcg cggcctccca cgtcgacgcg ctgctgatcc tcaagaccga gaaccggcgg
     1141 tacgtgacgg ggttcacggg ctcggccggc ctggcgctcg tcaccgggat ggaggcgctc
     1201 ctcgcggtcg attttcgcta cgaagagcag gccgacgccg aggcgcccgg ggccaccgtg
     1261 atccgggggg ggcgggaccc gctcaaggcg ctcgccgccg ccgtggaagg ccgggggctc
     1321 tcgcggatag gctttgaggc cgagttcgtc ccctacgccc aggtcgcccg attgcgggag
     1381 aaactcgccc ccgcggagct ggtcgccctg agcgacgtcg accggctgcg ctgggtgaag
     1441 gacccggcgg aggtcgcggc gatttcccgc gccgccgaga tcgcggacgc ggcgtacctt
     1501 cgggtgctgg agatgctgcg cccgggcgtc tccgagcggg gcgcggcggt ggagctggaa
     1561 acgttcatgc ggcgggccgg cgcggaccgg gtggcgtttg acagcgtgct cgcgagcgga
     1621 ccacgctccg cgctcccgca cggcctggcc accgagcggg tcatgggccc cggggagttc
     1681 gtgaccctcg actttggggc gatgtggggg gggtactgct ccgactgcac ccggaccgtc
     1741 gtcctgggga gcgccgacga gcaacagcgc cgggtctacg gcgtggtcct ggacgcccag
     1801 cgccgggccc tcgagatgat caagccgggg gtcgggtgcc gggacgtgga tgcgggcgcc
     1861 cgatccgcga tcgccgatgc gggatacgga gcggcgtttg gacactcgct ggggcatggc
     1921 gtcgggctgg aggtgcacga gggcccgcgg ctgtcccccc aggaggaggc cgtgctcgag
     1981 tccggcatgg tcgtcaccgt cgagccgggg gtctatctgc cggggtgggg cggggtgcgg
     2041 atcgaagata cggtcgtggt cacgcaggac ggatgccgca tcctcacgcg ggccccgaag
     2101 gagcttcacg tcctcccggt gtgagccgtt gacaggcctg tggagcgccc ggtacactcc
     2161 cgaggggtga tttggggtga tctccagcaa tgatttccgt cccggcgtcc acgtgctgct
     2221 cgacggcgag gtgtacgtca tcgttgcctc ggagcactcg aaggtcgcga tgagacacgg
     2281 cttggtcaag gccaagattc ggaacacgaa gacgggggtg acgacggagc ggacgttccg
     2341 cggaggcgaa cgggtgccgc tggtgcacct cgagctcaag acgatgcagt acctctacac
     2401 caccggcgac gagtacgtcg tgatggacaa gaccacgttc gagcagctcc cgctcccgcg
     2461 cgcgctcctc ggggatgcgg tccggtttct cgctgaaaac accgacgtga cggtcgtctt
     2521 ccacgaggac gcgccgatcg gggtggagct gccgaccgcg gtggagctgc gcgtcgtcga
     2581 gaccgccccc gggtttcggg gggatacggt gagcggaggg accaagccgg ccacgctcca
     2641 aaccggggcc gtcgtgcagg tgccattgtt cgtcacggcc ggcgaggtcg tccggatcga
     2701 cacccgcacc ggcacctacc tcgagcgcgt ccggtaggct gcccgcaact tcccgtttgc
     2761 ccggtcggag cacgtgtgat attatggaac gagcgtttac ccagaggtgg gggcatggaa
     2821 ttagagggcg caacgacgca gccggtgacc gtgagcgagt ttcagcggca ccccaaggat
     2881 tccgggtctc cggatgtgca gatcgcccgc ctgaccgagc gcatcaatca cctg
//
LOCUS       DASQGH010000450         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35948, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000450 DASQGH010000000
VERSION     DASQGH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35948"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEW91_08840"
     CDS             <1..227
                     /locus_tag="VEW91_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HYM91724.1"
                     /translation="DIQRRLFAPDLPDPDLLIRTGGEHRLSNFLLWQAAYAELYFTDV
                     HWPDFQKLDLLEAIRSFQARQRRFGGVELG"
     gene            220..1068
                     /locus_tag="VEW91_08845"
     CDS             220..1068
                     /locus_tag="VEW91_08845"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYM91725.1"
                     /translation="MDSGSPPGSVGAGGGLAARLGTAAIGVPLILLLVVLGTPWLFVG
                     VAGAIVVAMAEFYRMVERAGYRPVWEAGMAAGIVFALEAAWPQPWQGVVLPALLIYAV
                     ADHLRRSRPERVLADTALTVLGAVYVGYLMSFTLRLRALPDGPAHAAGPAPALLVILT
                     VWAADSAAYFVGLAAGRRKLLPQVSPNKSLEGALGGVAAGIVAGLVCGWAFRFALPMA
                     AAIGGLCAVSSVVGDLWESAIKREVGVKDAGRILPGHGGFLDRFDGLLFAMPVGYFAL
                     RWWPAP"
     gene            1158..2207
                     /locus_tag="VEW91_08850"
     CDS             1158..2207
                     /locus_tag="VEW91_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HYM91726.1"
                     /translation="MSTFVLAILALQLMILVHELGHLVVAKRVGITVYTFAIGFGPRL
                     AAFTWRETTYALNMLPIGGYVNMAGEDLDGRQPDVPPERLFRTKSVAERLAVVCAGPV
                     MNFLLAILLLAVVASAFGIPVGVSPRVGTLLPGYPAAEAGLRPGDEILAIDDQPMKDG
                     DTIVRTIHSSGGRTLALLIRRGNAQFVIHVPTRYDARQRVWLTGFSPAVIRRHLDPAR
                     ALGWGALTTGRDIVAYLGALGGLFRSGRLLNELSGPVTAVNVLGQAAHAGAETFLYIT
                     AFFSIIIGLFNLFPLPALDGGRAAFLVVEGLRRRPVDPRREGYIHLVGLGLLLCLIIA
                     LTVRDILHPVRLPLP"
     gene            2235..3323
                     /gene="ispG"
                     /locus_tag="VEW91_08855"
                     /gene_synonym="gcpE"
     CDS             2235..3323
                     /gene="ispG"
                     /locus_tag="VEW91_08855"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYM91727.1"
                     /translation="MAMDRTHTRRVQVGQVVTGGGAPVSVQSMTTTDTRDVDATVAQI
                     QALEAAGCEIVRVAVPDKTAAAALGRIKARIRLPLVADIHFDYRLALAALEAGVDKLR
                     LNPGNIGVADRVRAVAREARARGVPIRVGANVGSLSKEMLRKYGEPCADALVESASEE
                     IKILEDLDFRDIVISVKASDVGMAVEAYRKIAELAPYPLHVGITEAGTTWAGTVRSSV
                     GLGAILSSGIGDTIRISLAADPVEEVKVGFEILKSLGLRMKGPILVACPSCGRADVDI
                     VALAEEVERRLQQYPVPVKVAVMGCAVNGPGEARMADLGVAAGRGMGLIFKKGKIIAS
                     LPEARLLEGLMKEVEAVVREKQEAERSQ"
     gene            3361..>3888
                     /locus_tag="VEW91_08860"
     CDS             3361..>3888
                     /locus_tag="VEW91_08860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYM91728.1"
                     /translation="MIFPLRDDIPSRTFPVMMYLVMVACGAAFFYTMALPSLDAMARL
                     FTTYGVVPTRFTGHGGSGPVALPVPLVTSLFLHGGWAHLLGNMLFLWIFADNVEDAMG
                     HGPFVVFYLICGVVANLAHILANPLSGEPTIGASGAIAGVLGAYLVLYPRAHVQLMVW
                     LLIFYVRVIWVPAVLF"
BASE COUNT          503 a         1285 c         1427 g          673 t
ORIGIN      
        1 ccgacatcca acgccgcctg ttcgcccccg acctccccga tcccgatctg ctgattcgca
       61 ccggggggga gcaccggctc agcaactttc tgttgtggca ggccgcgtac gcggaactct
      121 acttcaccga tgtccactgg cccgactttc agaagctgga tctcctcgag gcgattcgga
      181 gcttccaggc ccggcagcgg cggtttgggg gtgtggaact tggatagcgg ctcccccccc
      241 ggctctgtcg gcgccggcgg cggtctggcc gcccgattgg ggacggcggc gattggggtt
      301 cccctgattc tgctgctcgt ggtgctcggc accccctggc tcttcgtcgg cgtcgccggg
      361 gcgattgtcg tcgcgatggc cgagttctac cgcatggtcg agcgcgccgg gtaccggccg
      421 gtctgggaag ccgggatggc ggcggggatt gtgtttgcgc tcgaggccgc ctggccgcag
      481 ccgtggcagg gggtggtcct cccggcgctc ctcatctacg cggtcgccga tcacctccgg
      541 cgcagccgcc ccgagcgcgt gctggcggac acggcgctca ccgtgctcgg cgcggtgtac
      601 gtgggttacc tgatgagctt cactctccgg ctgcgcgccc tcccggacgg gccggcccat
      661 gcggcgggcc cggcgccggc cctgctggtg atcctcacgg tgtgggcggc ggacagcgcg
      721 gcctatttcg ttgggctggc ggccggcagg cggaaactcc tgccccaggt cagcccgaac
      781 aagtcgctgg agggggccct ggggggtgtg gccgccggga tcgtcgccgg cctggtctgc
      841 ggctgggcgt tccgcttcgc gcttccgatg gccgctgcga tcggggggct gtgcgccgta
      901 tccagcgtgg tgggcgacct gtgggagtcg gcgatcaagc gggaggtcgg ggtcaaggac
      961 gcgggacgga tcctccccgg gcacggcggc ttcctggacc ggttcgatgg cctcctgttt
     1021 gccatgcccg tgggctactt cgccctgcgg tggtggcccg cgccctagcg gcgggaaacc
     1081 gggccaaacc gcgggcgaga agggaaccta gagaagggta gatgcgtagc ggagcgcaca
     1141 aggtaggggg agtctgagtg tcgacgttcg tgctcgcgat cctggcgttg caactcatga
     1201 tcctggtcca cgagctgggc cacctggtcg tggccaagcg cgtggggatc accgtgtaca
     1261 cgttcgcgat cgggttcggc ccccggcttg cggcgttcac ctggagggaa acgacctacg
     1321 cgctcaacat gctgcccatc ggcgggtacg tcaacatggc cggcgaggat ctcgatggcc
     1381 ggcagccgga cgtgccgccg gagcgcttgt tccggacgaa atccgtggcc gagcgcttgg
     1441 cggtcgtctg cgcaggcccg gtgatgaatt tcctgctcgc catcctgctg ctggccgttg
     1501 tcgcctccgc gttcgggatc cccgtggggg tgagcccccg cgtgggcacg ctgctccccg
     1561 gctatccggc cgcggaggcg ggccttcgcc cgggggatga gatcctcgcg atcgatgacc
     1621 agccgatgaa agacggcgac acgatcgtcc ggaccatcca ctcgagcggc gggcggacgc
     1681 tggcgctgct gattcggcgg gggaacgccc agttcgtgat ccacgtgccc acccggtacg
     1741 acgcgaggca gcgggtctgg ctcacggggt tctcgcccgc ggtgatccgc cggcatctcg
     1801 atccggccag ggccctgggg tggggggcgc tcaccacggg ccgcgatatc gtcgcgtacc
     1861 tcggcgccct cggtggcctg ttccgctccg ggcggctctt gaacgagctc agcgggcccg
     1921 tcaccgcggt gaacgtgctc ggccaggcgg cccacgcggg ggcggagacc ttcctctata
     1981 tcacggcgtt cttcagcatc atcatcgggc tgttcaacct cttcccgctc cccgccctcg
     2041 acggcggccg ggcggcgttc cttgtcgtgg aggggctccg ccggcggccg gtggatccgc
     2101 gccgggaggg gtacattcac ctggtgggcc tcgggctgtt gctgtgcctg atcatcgcgc
     2161 tgacggtccg ggacatcctc cacccggtgc gcctgccgct cccgtaatgc tacaatagga
     2221 agccgacatc ggggatggcg atggatcgca cacacacgcg acgggtgcaa gtcgggcagg
     2281 tggtgaccgg gggcggcgcg ccggtctcgg tccagtcgat gacgacgacc gatacccgcg
     2341 acgtcgacgc caccgtggcc cagattcaag ccctcgaggc cgcggggtgc gagatcgtgc
     2401 gcgtcgcggt gccggacaag accgcggccg ccgccctcgg ccggatcaag gcccggatcc
     2461 ggctgccgct ggtggccgac atccacttcg actaccgcct cgcgctggcc gcgctcgaag
     2521 ccggggtcga caagttgcgg ctcaacccgg gcaacatcgg ggtcgccgat cgggtccgcg
     2581 ccgtggcccg ggaggcgcgc gcccgagggg tcccgatccg ggtgggagcc aacgtggggt
     2641 ccctctcgaa ggagatgctc cggaagtacg gcgagccgtg cgccgatgcc ctggtggaga
     2701 gcgcatcgga ggagatcaag atcctcgagg acctcgactt tcgcgacatc gtgatctccg
     2761 tcaaagcgag cgatgtgggg atggccgtcg aggcctaccg gaagatcgcc gagctcgccc
     2821 cctatccgct ccacgtgggg atcaccgagg ccgggacgac ctgggcgggg accgtgcggt
     2881 cctcggtcgg gctgggggcg atcctcagca gcgggatcgg ggacacgatc cggatctccc
     2941 tggccgccga tccggtggaa gaggtgaagg tcgggttcga gatcctgaag tcgctcggcc
     3001 tgcgtatgaa ggggccgatc ctggtggcct gcccgtcgtg cggtcgggcc gacgtggata
     3061 tcgtggcgct cgccgaggag gtggagcgcc gcctccagca gtacccggtg ccggtgaagg
     3121 tcgccgtcat ggggtgcgcg gtcaacggtc ccggggaggc gcggatggcc gacctcggcg
     3181 tcgcggccgg ccgggggatg gggttgatct tcaagaaggg caagatcatc gccagcctgc
     3241 ccgaggcgcg gctcctggag gggctgatga aggaagtgga agccgtggtg cgcgagaagc
     3301 aggaggccga gcgctcgcag tgaccgggcg gttcccgcat ccccgccctg acccaggccg
     3361 gtgatcttcc ccctccgcga cgatattccg tcccgcacgt tccccgtcat gatgtacctt
     3421 gtgatggtcg cgtgcggggc ggcgtttttc tacaccatgg cgctcccatc gctcgacgcg
     3481 atggcgcgcc tgttcaccac ctacggcgtg gtcccgacgc ggtttaccgg ccacggggga
     3541 agcgggccgg tcgcgctccc cgtcccgctc gtgaccagcc tgttcctgca cggcggctgg
     3601 gcgcacctgc tgggcaacat gctgtttctc tggatcttcg cggacaacgt cgaggatgcc
     3661 atgggccacg ggccgtttgt ggttttctac ctgatctgcg gcgtcgtggc aaacctcgcc
     3721 cacatcctcg ccaaccccct gtccggtgag ccgacgatcg gcgcgagcgg ggcgatcgcc
     3781 ggggtgctgg gggcctacct ggtcctgtac cctcgggcgc acgtgcagct gatggtgtgg
     3841 ttgctgatct tctatgtgcg ggtgatctgg gtgcccgcgg tgttgttc
//
LOCUS       DASQGH010000451         6183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55644, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000451 DASQGH010000000
VERSION     DASQGH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55644"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEW91_08865"
     CDS             <1..370
                     /locus_tag="VEW91_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding oxidoreductase"
                     /protein_id="HYM91729.1"
                     /translation="GYWEQQGWDHEAIVKTNSAFRVETKDGAALALGGWAFAGNRGIS
                     KVEFSPDGGTTWLPAAVKEPLGENCWQFWSATWTPPRPGEYTIKVRATDGKGALQPEE
                     PKPTLPDGGQGYHTVKVHFT"
     gene            554..1087
                     /gene="def"
                     /locus_tag="VEW91_08870"
     CDS             554..1087
                     /gene="def"
                     /locus_tag="VEW91_08870"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYM91730.1"
                     /translation="MTPRPRAGRVRGLGAMRGRARRIVTLGDRDAAVLRRRCRPVPVV
                     DRTIRQLVADMLVSMRHAGGIGLAAPQIGVPLRVVVADTDAGPLAVVNPRLRWRSGTQ
                     VGVEGCLSIPGVFGDVRRARRIEVEGRNVRGRRVVLRSRDLLARVFQHEVDHLNGVLF
                     IDPGRLLRRRRRPRRIR"
     gene            1084..1506
                     /locus_tag="VEW91_08875"
     CDS             1084..1506
                     /locus_tag="VEW91_08875"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HYM91731.1"
                     /translation="MTKAERYFTLGEATALLPRLTDLVGALRRLRDDAVVKKAQVDLL
                     WRRLEGGEPVLSVIGEEQRQLDAISARLVSAAEEVESTGCVLRDLDMGLVDFPFRIRA
                     GVVFLCWRVGEPAIQFWHGTDEGYGGRKPIGRLPLDQA"
     gene            1509..2912
                     /gene="lpdA"
                     /locus_tag="VEW91_08880"
     CDS             1509..2912
                     /gene="lpdA"
                     /locus_tag="VEW91_08880"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYM91732.1"
                     /translation="MVRSSEPYDLIVIGGGPAGYVGAIRGAQLGLRTALVEREKLGGT
                     CLHVGCIPTKVLLHTAQLLEEMRTGGELGIQVSDLRLDYEQVRRRMERVITTNFRGVE
                     FLMRKHGVAVFPGDGRLLGPARVGVAATDGSDLTLEGRHILLATGSRPQTLPGVVIDN
                     HRVLDNAGVLQLPAVPRSLAILGAGAVGTEFASLFAAFGAKVTLIEMMPTILPLEDED
                     VSRVVAHELSRRGVAIRSRTAVTGVTASADGVRIAVRAGAAEETIEAEYALVAVGRAP
                     VVEGLGLDAVGLEPRRGGIPVDGGMRTAVPSIFAAGDVIGGLLLAHVASAEATVAVEA
                     IAGQEPRGLDPMLMPRATYSIPQVASCGLTEREARERGREVAVGRFPFTGNSRAAILG
                     RREGFVKIVADRALGEILGIHMVGPEVTELLPEGVLGKALEATVREIGQAVHAHPTLS
                     EAVKEAALAALGRAVHG"
     gene            3136..4092
                     /locus_tag="VEW91_08885"
     CDS             3136..4092
                     /locus_tag="VEW91_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYM91733.1"
                     /translation="MYYYLCLARAVDERMWALQRSGKAAFVISGQGHEGAQVGVVYAL
                     DRERDWLVPFYRSVAAVLMKGMPPAELFLIQLGRDTDPSSGGRQMPGHYGHSRHKILS
                     TSSPVATQLLHAAGIAYAAKVRGTGEVCLTECGEGATSEGDFHEALNFAGVHRLPVVF
                     VVENNGYAISVPVSQQMAVANVSSRAAGYGMPGVTVDGSHVLQVYGAAWEAVARARSG
                     GGPTLLEVTVPRLTPHSSDDRQEKYRPAEELAHLWERDPVRVFTAELRERGVLTHGRE
                     QEIRARIEHEVDAGTEIAEAAPLPDPASAVRYVYAEPPDAGR"
     gene            4095..5078
                     /locus_tag="VEW91_08890"
     CDS             4095..5078
                     /locus_tag="VEW91_08890"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYM91734.1"
                     /translation="MADKRFLDAIHDALLEEMTRDPAIVVLGEDVGKKGGVFGVTDGL
                     YEAFGEARVIDTPLAESCIVGVAIGMSVNGLRPVAEIQFADFIHPAFDQILSEAARLR
                     YRSKNDFGCPIVIRTPFGGGVHGALYHSQSIEAFYAHIPGLKVVVPYTPADAKGLLKA
                     AIRDPDPVLFLEHKKMYRSVRGEVPDAEFTVPIGPAAVRRPGRDLSIFAYGLMAHESL
                     QAADLLSREGIEAEVVDVRTLRPLDTKTILESVERTNRALIVHEDNRFGGFGAEIAAT
                     IAEAGFRHLDAPPTRLCGPDVPAVPFSPPFEEWFMISAQKIADAARAVVAY"
     gene            5111..>6183
                     /locus_tag="VEW91_08895"
     CDS             5111..>6183
                     /locus_tag="VEW91_08895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYM91735.1"
                     /translation="MPTEIKMPQLGESVHEGTIGKWLKRPGDPVAKYEPLVEVITDKV
                     NVEMPSPFAGVLREVLVAEGVTVVVGTPIAVIEEQPRAAAPIGPAPVLVAPPPAAPAT
                     PAAGGRLHAPVAARPAAAAAKLTPLVRRLVEEHQIGADEMATIAGSGEGGRITKEDVL
                     RYLDTRTPASRGAVAAAPAPAPPAAAPLGAGDQLHPLSPLRRTIAERLTRSKREIPHA
                     YGIIDVDLTTLVRHREAHKAAWQVREGANISITAFIVRAATRALRAFPTVNATFTPEG
                     ILYKHAIHFGVGVAIPDGLIVPVIKHADQKTVVQLAKELTELAARAREGKLTLDDVSG
                     GTFTLTNAGVFGSVYSMPIINYP"
BASE COUNT          925 a         2099 c         2253 g          906 t
ORIGIN      
        1 tgggtactgg gagcagcagg ggtgggacca cgaggccatc gtcaagacca acagcgcgtt
       61 ccgcgtggaa accaaggacg gcgccgccct cgccctcggc gggtgggcgt tcgccggcaa
      121 tcgcggaatc tccaaggtgg agttcagccc cgatggcggc acgacgtggc tcccggccgc
      181 ggtcaaggag ccgctcgggg agaactgctg gcagttctgg tccgctacct ggacgccccc
      241 gcgtcccggc gagtacacga tcaaggtgcg tgcaacggac gggaagggag cgctgcagcc
      301 cgaggagccg aagccgacgc ttcccgacgg ggggcaaggg taccacacgg tgaaggtgca
      361 ctttacgtag ccgcatccgc tcgaggtagt gtgcccccgc gggaaccgcg ggcgcgggca
      421 aggatttttc gggtcggtca ccaacatact tccagggatc acctttggag agatgccgcg
      481 ggcctttccc gtcgacggtc gtttcgccag ccctccgcct gccttgcctc cgcggcgccc
      541 caggatctga cccgtgaccc ccaggccgcg cgcgggacgt gtcagggggc tcggcgcgat
      601 gcgcggccgc gcccggcgca tcgtcaccct cggcgatcgc gatgcggccg tgctgaggag
      661 gcgatgccgg cctgtgcccg tcgtcgaccg tacgattcgc caactcgttg ccgacatgct
      721 ggtcagcatg cggcacgccg gcgggatcgg cctcgccgcg ccccagatcg gcgtccccct
      781 gcgcgttgtg gtcgccgaca ccgacgcagg accgctggcg gtggtgaacc cccgccttcg
      841 gtggcggagc ggcacgcagg tgggggtcga agggtgcctc agcatcccgg gggtcttcgg
      901 ggacgtccgt cgggcccgcc ggatcgaggt cgagggccgg aacgtgcggg gccgccgggt
      961 cgtgctcagg agccgcgatc tcctcgcccg cgtgttccaa cacgaggtcg accacctcaa
     1021 cggcgtgctg ttcatcgacc ctgggcggct gctccgccgc aggcggcgcc cccggcgcat
     1081 ccggtgacca aagcggagcg ctacttcacc ctcggcgaag cgacggccct gctcccccgg
     1141 ctcaccgatc tggtcggcgc gctgcggcgg ctccgggatg acgcggtggt caagaaggcg
     1201 caggtcgacc tgctgtggcg gcgcctggaa ggcggggagc cggtcttgag cgtgatcgga
     1261 gaggagcagc gccagctgga tgccatttcg gcccgcctcg tctccgcggc cgaggaggtc
     1321 gaatcaaccg ggtgcgtgct gcgcgatctc gatatggggt tggtcgattt cccattccgg
     1381 atccgggccg gggtggtgtt cctgtgctgg agggtgggcg agcccgcgat ccagttctgg
     1441 cacggcaccg acgaagggta cggcgggcgc aaacccatcg ggcggcttcc gctcgaccag
     1501 gcgtgagcat ggtccgctcg agcgagccgt acgatctgat cgtcatcggg ggcggccctg
     1561 ccgggtacgt gggcgcgatc cgcggcgccc agctggggct gaggacggcc ctggtcgagc
     1621 gggagaagct cggcggcacc tgcctgcacg ttggctgcat ccccacgaag gtgctcctgc
     1681 acaccgccca actcctggag gagatgcgga cgggcgggga gctggggatc caggtctccg
     1741 acctccggct cgattacgaa caggtgcgcc gccggatgga gcgggtgatc acgacaaact
     1801 tccgcggcgt cgagtttttg atgcgcaagc acggcgttgc ggtcttccca ggggatgggc
     1861 gactccttgg gcccgcccgt gtgggggtcg ccgccaccga tgggtctgat ctcaccctcg
     1921 aaggacggca catcctcctc gcgaccggat cgcgccccca aacgctcccc ggcgtcgtca
     1981 tcgacaacca ccgcgtgctc gacaacgccg gcgtgctgca gctcccggca gtcccccggt
     2041 cgctcgcgat cctcggcgcc ggggcggtgg ggaccgagtt tgccagcctc tttgccgcct
     2101 tcggcgccaa ggtaacgctc atcgagatga tgccgacgat cctcccgctc gaggatgagg
     2161 acgtctcgag ggtggtcgcc cacgaactga gccgccgggg cgtcgcgatt cgcagccgca
     2221 ccgcggtgac cggcgtcacc gcctctgccg acggcgtgcg gatcgcggtg cgcgccggcg
     2281 ccgccgagga aacgatcgag gccgagtacg ccctggtggc ggttggccgc gccccggtgg
     2341 tcgagggcct cgggctggat gcggtcgggc ttgagcctcg gcggggaggc attccggtgg
     2401 acggagggat gcggaccgcg gtgccatcga tcttcgctgc cggagacgtc atcggggggc
     2461 tgctgctcgc acacgtggcg tcggccgagg cgacggtggc cgtggaggcc attgctggcc
     2521 aggagcctcg cgggctcgac ccgatgctca tgccgcgggc gacctactcc attccccagg
     2581 tcgccagttg cggcctgacg gaacgggagg cgcgcgaacg cgggcgcgag gtcgcggtcg
     2641 gccgctttcc cttcaccgga aacagccggg cggccatcct cgggcgccgc gaaggcttcg
     2701 tgaagattgt cgcggaccgc gcgctcggcg agattttggg tattcacatg gtggggccgg
     2761 aggtgaccga actgttgccc gagggcgtgc tcgggaaagc cctcgaagcg acggtgcggg
     2821 agatcggtca ggcggtccat gcgcacccca cgctctcgga ggcggtgaag gaagccgcgc
     2881 tggccgcact cggccgtgcc gtgcacgggt gacccctgtg gagcgttccc cgaagggggc
     2941 ccgcatctcg cacgcgaggg gaggagcacc ctcgccgtac ggggaagact ctcttcgagg
     3001 gcgccgcggg gcacgcagca cacggatggg ggaggttgca tgagcgcaaa ggagaccacg
     3061 atccgtccgt ccccgggtcg gggcgcggcg gcgccccgag gggccaagga ccccagcccc
     3121 gccgagctgc tcgagatgta ctactacctc tgcctcgccc gcgccgtaga cgagcggatg
     3181 tgggcgctcc aacggtcggg gaaagccgcg ttcgtgatct ccggccaagg gcacgagggc
     3241 gcgcaggtcg gggtcgtgta cgcgctggat cgggagcggg actggctggt gcccttctac
     3301 cggtcggtcg ccgccgtcct gatgaagggc atgccgccgg ctgagctctt cctgatccag
     3361 ctcggcaggg acaccgatcc cagcagcggt ggccgccaga tgccgggaca ctacggccac
     3421 tcccgccaca agatcctgtc caccagcagc ccggtcgcca cccagctgct ccacgcggcc
     3481 gggatcgcct acgcggcgaa ggtgcggggg accggcgaag tgtgcctcac cgaatgcggc
     3541 gagggtgcga ccagcgaagg cgattttcac gaggcgctca acttcgcggg cgtgcaccgg
     3601 ctccccgtcg tgttcgtggt ggagaacaac gggtacgcga tctcggtgcc ggtgagccag
     3661 cagatggccg tcgccaacgt ctcatcccgg gcggccggat acggcatgcc cggcgtgacg
     3721 gtggacggca gccacgtgtt gcaggtgtac ggggcggcct gggaggccgt cgcgcgcgcc
     3781 cgcagcggag gggggccgac gttgctcgag gtcaccgtgc cccgcctcac cccgcacagc
     3841 agcgatgacc ggcaggagaa gtatcggccg gccgaggagc tcgcccacct ctgggagcgg
     3901 gatcccgttc gggtcttcac cgccgaactg cgcgagcgcg gggtgctcac ccacggcagg
     3961 gagcaggaga tccgggcgcg gatcgagcac gaggtcgatg cgggcaccga gatcgccgag
     4021 gccgccccgc tgcccgaccc ggcgtcggcc gtccggtacg tgtacgccga accgcccgac
     4081 gcgggccggt agccgtggcc gacaagcgct tcctggacgc gatccacgat gcgctcctcg
     4141 aggaaatgac ccgggatccc gccatcgtgg tgctgggaga ggacgtcgga aagaaggggg
     4201 gcgtgttcgg ggtcaccgac gggctgtacg aggcgttcgg cgaggcgcgg gtgatcgaca
     4261 ctccgctcgc ggagtcgtgc atcgtggggg tggcgatcgg gatgtcggtc aacgggctgc
     4321 gtcccgtggc cgagatccag ttcgccgatt tcatccaccc cgcgtttgat cagatcctca
     4381 gcgaagcggc ccgattgcgg taccgctcca agaacgactt cggatgtccg atcgtcatcc
     4441 gcaccccgtt cggcggcggc gtgcacggcg cgctctacca ctcacagagc atcgaggcgt
     4501 tctacgcgca cattcccggg ctcaaagtgg tcgtgcccta cactcccgcg gatgcgaagg
     4561 gcctcctcaa ggccgcgatc cgggatcccg acccggtgct cttcctcgag cacaagaaga
     4621 tgtatcgctc ggtgcggggg gaggtccccg acgccgagtt cacggtcccg atcgggccgg
     4681 ccgcggttcg gcggcccggg cgggacctca gcatctttgc ctacgggttg atggcccacg
     4741 aatcgctgca ggccgccgac ctgctcagcc gggaagggat cgaggccgag gtcgtggacg
     4801 tacggacgct ccgccccctc gacacgaaga cgatcctcga aagcgtcgag cggacgaatc
     4861 gggcgctcat cgtccacgag gacaaccggt ttggcggctt cggcgcggag atcgccgcga
     4921 cgattgccga ggccgggttc cgccacctcg acgcgccgcc gacccggctc tgcggccccg
     4981 acgtgcccgc ggtgccgttt tctccgccgt ttgaggagtg gttcatgatc tccgcccaga
     5041 agatcgccga cgcggcccgg gcggtggtcg cctactagga ttcgagctgg ctgggatcgg
     5101 ggggctgcgg atgccgaccg agatcaagat gccccaactc ggcgagagcg tgcacgaggg
     5161 gacgatcggg aagtggctca agcggcccgg agatccggtc gcgaagtacg agcccctggt
     5221 cgaggtcatc acggacaagg taaacgtcga gatgccgtcg ccgttcgccg gagtgctgcg
     5281 ggaggtgctg gtggcggaag gcgtcaccgt ggtcgtcggg acccccatcg ccgtcatcga
     5341 ggagcagccc cgggccgccg ccccgatcgg tcccgccccc gtgcttgtcg caccgccccc
     5401 ggctgcgccg gcgacaccgg ccgccggcgg gcggctccac gcgccggtgg ccgcgcgtcc
     5461 ggcagccgcg gcggcgaagc tgacgccgct tgttcgccgc ctggtcgagg agcaccagat
     5521 cggtgccgac gagatggcaa cgatcgcggg gagcggggag gggggccgca tcaccaagga
     5581 ggatgtgctc cgctacctcg acacccgcac gccggcgtcc cgaggcgcgg tggccgccgc
     5641 accagcccca gcgcccccgg ccgccgcccc gctaggcgca ggcgaccagc tccacccgct
     5701 ctcgcccctt cgccggacga tcgcggagcg attgacgcgg agcaagcggg agattcccca
     5761 cgcctacggg atcatcgacg tcgatctcac caccctggtg cggcaccgtg aggcccacaa
     5821 ggcggcgtgg caggtccggg agggtgccaa catctcgatc accgcgttca tcgtgcgcgc
     5881 cgcgacccgg gccctcaggg cgtttccgac ggtgaacgcg acgttcaccc cagaggggat
     5941 cctctacaag cacgcgatcc acttcggcgt gggggtcgcg attcccgatg ggctcatcgt
     6001 cccggtcatc aagcacgcgg accagaagac cgtggtccaa ctggcaaagg agctcaccga
     6061 gcttgcagcc cgcgcgcgcg aagggaagct gacgctggac gatgtgtccg gggggacctt
     6121 caccctgacc aacgcggggg tgtttgggtc ggtctactcg atgccgatca tcaactaccc
     6181 gca
//
LOCUS       DASQGH010000452         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27381, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000452 DASQGH010000000
VERSION     DASQGH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27381"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VEW91_08900"
     CDS             <1..489
                     /locus_tag="VEW91_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HYM91736.1"
                     /translation="DLAGRGWDQVAANGGIAALAAAVHGLSGSPLAFGAAAGAIAAAT
                     ADTWATEAGRWSPIPPRLITTGAVVPHGRSGGVTPVGTLASAGGALLIAGVAAALAGR
                     PLLSPVLIAVLAGGFTGALLDSVLGATIEGRLRWVDNSLINFLATAWGAGVVLAGAGW
                     WR"
     gene            complement(696..2258)
                     /gene="purH"
                     /locus_tag="VEW91_08905"
     CDS             complement(696..2258)
                     /gene="purH"
                     /locus_tag="VEW91_08905"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYM91737.1"
                     /translation="MTITRGLLSVWDKTGIVEFARGLADLGVELLSTGGTAAALRGAD
                     VPVVELSDVTGFPEILGGRVKTLHPAVHGGLLAVRGNPAHTAELARHGIRPIDLVAVT
                     LYPFEAAIASGVDLAGALEQIDVGGVALLRAAAKNFGDVVVLSRPSQYGEVLDELRRT
                     GTVGRDTRARLAGEAFARTSEYDAAIARYLDRQGAGGIFPQHLLLAFEKAQDLRYGEN
                     PHQRGAFYRDPGVAGSCVATARQLAGKPLSYNNIYDLDAALQLVQEFGEPSAVIVKHG
                     IPCGVGTGKALREAYLHAREGDPVSAFGGVVALNRPVDRATAEALAETFLEAVIAPGF
                     GEDALAALRQKKNVRLMEVSTPPAPSPAPAGSGGDLLDLRRVRGGLLVQERDAVDLVE
                     ANLKVATSRAPTAQEWTDLRFAWTVCRYVRSNAIVLARDRQVVGIGAGQMNRVEAVRL
                     ATRAAGERARGAAMASEAFFPFPDALEMAIHAGVTAVIHPGGSVRDAEVTAAAESSGI
                     AMVLTGIRHFRH"
     gene            complement(2255..2917)
                     /gene="purN"
                     /locus_tag="VEW91_08910"
     CDS             complement(2255..2917)
                     /gene="purN"
                     /locus_tag="VEW91_08910"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYM91738.1"
                     /translation="MPEPLRVGVLVSGAGSNLQALIDACRAGAIPAEVVVVVSNVPTA
                     LALERARAGAVPAVVVDHRAYATRAAFEAALRGALEAHRVQLVCLAGFLRILSRDFVA
                     AFSGRIMNIHPALLPAFGGKGMYGERVHRAVLASGARQSGCTVHFVTEVPDGGPIIAS
                     VTVPVEAGDTPATLAARVARAEHRLYPLAVRWFAEGRLRLEGDQVAILPPAGSGVASE
                     AR"
     gene            complement(2910..>3286)
                     /locus_tag="VEW91_08915"
     CDS             complement(2910..>3286)
                     /locus_tag="VEW91_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HYM91739.1"
                     /translation="ALLKPHRPYAHPVLALRTRVEICGIAHVTGGGIPGNLVRILPEG
                     CRARIARGRWPIPPLFSVIQKRGKIADDEMFRTFNMGLGLLLVVRESGSASAVAHLEK
                     AGERAWIVGRIIASSRGVEIGA"
BASE COUNT          502 a         1203 c         1129 g          452 t
ORIGIN      
        1 gacctcgcgg ggcgggggtg ggaccaggtg gccgccaacg gggggatcgc cgccctggcc
       61 gctgctgtac acgggctgtc cggatcgccg ctggcattcg gggcggcagc cggagcgatc
      121 gctgcggcca ccgcagacac ctgggcaacg gaggcggggc gctggagccc gatcccgccc
      181 cggctcatta ccaccggggc ggtggtgccg cacggccggt cagggggcgt cacgccggtt
      241 ggaaccctag cgtccgcggg aggagcgctc ctcatcgccg gggtggcggc agccctcgcg
      301 ggaaggccgc tcctgtctcc ggtgctgatc gccgttctgg cggggggatt caccggggcg
      361 ctactggaca gcgtcctcgg agcgacgatc gagggccgcc tccgctgggt cgacaactcg
      421 ctgatcaact tcctcgccac cgcgtggggc gccggagtcg tcctcgcggg agcgggttgg
      481 tggcgctaga aaatataaaa gccgacctct cacgagaatc ggcagtagca ttgccccatt
      541 ggactttgcc tggtcagcgt tgctgaaacg tattataccc cccacccggt gaaatgtcaa
      601 acattgttta aaacgcgtca acccatgcag aaccggaggt tgctccgcgg acacagcgtc
      661 cgcaccggcc tgggtgagcg cggggcttcc cgtgatcagt gcctgaagtg ccggatcccg
      721 gtcagcacca tcgcgatccc ggaggactcc gccgcagcgg tcacctcggc gtcccggacc
      781 gagccgccgg gatggatcac cgcggtgacg ccggcgtgga tcgccatctc gagcgcgtcg
      841 gggaacggaa agaacgcctc ggaggccatg gccgcgcccc gggcgcgctc gccagccgcc
      901 cgcgtggcca ggcgaaccgc ttcgacgcga ttcatctggc cggcaccgat ccccacgacc
      961 tgacggtcgc gcgcaaggac aatcgcgttg gagcggacgt accggcacac cgtccaggcg
     1021 aagcgcagat cggtccactc ctgcgcggtg ggcgcgcggg aggttgccac cttgaggttg
     1081 gcctccacca ggtcgaccgc atccctctcc tggacgagga ggccgccccg cacgcggcgc
     1141 aggtcgagta gatcaccgcc tgaccctgcc ggagccggag aaggcgccgg gggtgtcgac
     1201 acctccatca gccgcacgtt cttcttctgc ctgagcgccg ccagggcgtc ctcgccgaac
     1261 cccggggcga tcaccgcctc cagaaatgtc tcggccagcg cttcggcggt ggcccgatcc
     1321 accggccggt tgagtgcgac gaccccgccg aacgcggaga ccgggtctcc ctcccgcgcg
     1381 tgcaggtacg cctcgcgcag cgctttcccc gtcccgaccc cgcaggggat cccgtgcttg
     1441 acgatgacgg cggagggctc cccaaactcc tgtaccagtt ggagggcggc gtcaaggtcg
     1501 tagatgttat tgtacgaaag gggtttcccc gccagctggc gcgcggtcgc cacgcacgat
     1561 ccggcgacgc cggggtcgcg gtagaacgcg ccgcgctgat gcgggttctc cccgtagcgg
     1621 aggtcctggg ctttttcgaa ggcgagaaga agatgctggg gaaagatccc ccccgccccc
     1681 tgccggtcca ggtaccgggc gatcgccgcg tcgtattcgc tcgtccgggc gaacgcctcg
     1741 cccgcgaggc gcgcccgggt gtcccggccc accgtcccgg tccgccggag ctcgtcgagc
     1801 acctccccat actgggaggg ccggctcagg acgacgacat ccccgaagtt cttcgccgcc
     1861 gcgcggagga gcgcgacccc gccgacatcg atctgctcga gggcgcctgc gagatccacc
     1921 ccgcttgcga tcgcggcctc gaatggatac agcgtcaccg ccacaaggtc gatggggcgg
     1981 atgccgtgcc gcgccagttc ggccgtgtgc gccggattgc cccggacggc caggaggccc
     2041 ccgtggaccg ccgggtgcaa cgtcttcacc cttccaccca ggatctcggg gaaccccgtg
     2101 acatcgctca gctcgaccac cggcacgtcc gcgccgcgca gcgccgccgc ggtcccgccg
     2161 gtcgacagca gttccacgcc caggtcggcg agcccgcgcg cgaactcaac gatccccgtc
     2221 ttgtcccaga cgctcagcag cccgcgtgtg attgtcatcg cgcctccgag gcgaccccag
     2281 agcccgccgg cggcaggatc gccacctgat cgccctcgag ccgcagccgc ccttccgcga
     2341 accaccggac cgcgagcggg tacagccggt gttccgcccg ggcgacccgg gcggccagcg
     2401 tcgccggggt gtcccccgcc tccacgggaa ccgtcacgct ggcgatgata ggcccgccgt
     2461 cgggcacctc cgtgacgaag tgcacggtgc acccgctctg ccgggcgccg gacgcgagga
     2521 ccgcccggtg gacccgctcc ccgtacatcc cttttccccc gaacgccgga agcagcgccg
     2581 ggtggatgtt catgatgcgg ccagaaaacg ccgcgacgaa gtcccgggaa aggatcctga
     2641 ggaagccggc gagacagacg agttggaccc ggtgggcttc gagcgcccct ctgagcgccg
     2701 cttcgaacgc cgcccgggtc gcgtacgccc ggtgatcaac gaccacggcc gggaccgcac
     2761 ccgcgcgggc gcgctcgagc gccaacgccg tcgggacgtt gctgaccacg acgacgacct
     2821 ccgcggggat cgcgccggcg cggcacgcat cgatgagcgc ctgcaggttc gacccggcgc
     2881 cggagacgag gacgccgacc cgcagcggct caggcaccga tctcgacccc ccgcgaactg
     2941 gcgatgatgc ggccgacgat ccaggcgcgc tcgcctgcct tctcgaggtg cgcaaccgcc
     3001 gacgcgcttc cggactcccg gacgaccagg agcaggccga ggcccatgtt gaacgtgcgg
     3061 aacatctcgt catccgcgat cttccctcgc ttctggatca cggagaacag cggcgggatc
     3121 ggccaccggc cgcgggcgat gcgggcgcgg cacccctcgg gcaggatccg cacgaggttg
     3181 ccagggatcc cgcccccggt gacgtgcgcg atcccgcaga tctcgacgcg cgttcgcagg
     3241 gcgagcaccg ggtgcgcata cgggcggtgg ggtttgagca acgcat
//
LOCUS       DASQGH010000453         7130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1925, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000453 DASQGH010000000
VERSION     DASQGH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1527
                     /gene="secD"
                     /locus_tag="VEW91_08920"
     CDS             145..1527
                     /gene="secD"
                     /locus_tag="VEW91_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYM91740.1"
                     /translation="MRVGNSVRLLAVLLIVVAAFWTAFEPIVFPRGLATPTFQAPALH
                     LNLGLDLRGGSHIVLQARPTKDTPAITNDAMDGVLRVIRNRVDQLGVAEPSITRQGRD
                     RIVAELPGIENPQRAIELIGKTALLEFVDTQTQSLPQGAQWTDNDTVVIPDNKTTRKI
                     PKKVILTGADLVDARGQFDQFGRPNVAFTFKSRAAKTFEDYTTKNIGRYLTIVLDSRV
                     ISSPVINSPIPGGHGVIEGGFTLESARDLAVLLRGGALPLPVEVVENRTIGPLLGRDS
                     IDLSVRAGYIAIVLVALFMGLYYGLAGILADVALGVYTLMLFAVLTLIGATLTLPGIA
                     GFILSLGVAVDANVIIFEKVKEELRAGKTLRAAVGTGWARAIVTILDSNATTLIGALV
                     LLWLGTGPVRGFAVTLILGILTSIFTAIVVTRVFVDTTLETGAAGWIQRLGAVGTLRR
                     AARAAGGAGE"
     gene            1532..2587
                     /gene="secF"
                     /locus_tag="VEW91_08925"
     CDS             1532..2587
                     /gene="secF"
                     /locus_tag="VEW91_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYM91741.1"
                     /translation="MPQQAFNLIGRRRWWYALSLAVIVPGLIALYSHHVHDHHALNWG
                     VDFTGGNSLELRITQPFTVGEIRQIMGQFQLSDAIIQRSGDTEVFVRTRPLTQAQTNA
                     VIQAVTVRYPATKMLRVDTVGPEIGVELRNIAIFGVAIGIILQVIYISFRFHSIRFAI
                     AADIALLHDLLVVIGAFALTQREVNSSFLAVLLTVAGYSINDTIVIFDRIRENLGLRT
                     REPFEHLVNRSVLEALVRSINTAMTAVLAISAVYLFGGETIRDVAFGLVVSIITGGYS
                     SIFNASPILVDWHNWTERHRRPRDAQARTARIVERAERTPTVPQTGGDDPAPLRAGAP
                     GMPDEGGRRRRGGRRRR"
     gene            2599..2898
                     /locus_tag="VEW91_08930"
     CDS             2599..2898
                     /locus_tag="VEW91_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HYM91742.1"
                     /translation="MGLTVLWLLLVFIAATVFALSNSTTVTVNFWQWPVYTGPLALVV
                     IGAGVCGALLTYLGSLVHHMRQAQQIRSLRKSIQARETPMASPPPGRTEETHRPF"
     gene            2946..6449
                     /gene="recJ"
                     /locus_tag="VEW91_08935"
     CDS             2946..6449
                     /gene="recJ"
                     /locus_tag="VEW91_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HYM91743.1"
                     /translation="MSASPWIVAPPERGAAELAAVLGVRPLTAALLRRRGLSTVEDAR
                     RFLTPCLEDLANPRSLPGMDRAVERVAQAVQAGGRLAVHGDYDVDGISATAILVRGLR
                     LLGAEPLWYLPHRFHDGYGLGTRAVEMFAARGATLVIAADCGITAHEAVARARALRMD
                     AVILDHHAPTEERPAAIIVEPSAPLCAAGMAFMFVLALRERLGIADAVPAGMASLAAL
                     GTIADVVPLLDDNRRLAAAGLAEMRAAPLPGIKALAEIAGLTGPIGTWHVAWHLAPRL
                     NAPGRLGDPSPSLRLLLTDDPGEGRVLAQALDQANRERQEILDRTLTEAVAQAEQEAA
                     GAALVVAGEGWHPGVVGLVAGRLAEQYRRPVVAIGLEGDAGRGSGRSIPGFNLVGALQ
                     GCRGSLRGFGGHAMAAGLVIAREAVAEFRRAFTALAAVGAAERARTTGLVVEAEVGLD
                     DLTAAWVDELERLAPFGAANPEPVLAVRRVRALNRRVIGDGRHLRLDVSDGAASIEAI
                     GFAMAGPAELLTFTEAPVDLAVVPERDPFSPDRVRLRVVALEIPGADPEHILADTGAL
                     VDRLFSRAADYLGEVRYDGVEDAPALHTKVVGVTFDGRQQVLATLHPGSSLRLVREPA
                     NPHDPHAVQVATLDGRVLGYLRAPLAGRLSPSIDAGARYRVTVAAVTGGGERTLGVNI
                     FLEREDDADASREHAPARRRWDAGDTERLAAALNGGQPLAPVHAEVLRAAASGRPMVL
                     NFPPGRGPATALAGVAALGAREGRCTLVVAPLRRQVLHRADQLRSRLAPLGLRVRAVH
                     GLQGSRERARVDAALRAGGVDVLVASAEALAAGWPEAHADRVGTLVCDGVGGADLPPL
                     LRAFAGAQVCLMNVPAPAAAGESLGPGAVVIRDAGVRIGLRMVDRRSAADRDAVVEEV
                     LRGGEKTVVHAGGREACVQLAVRLRDRCGQHARLGYLHEGLPARVRQIIVQAFREGRL
                     GILVTTPALDEEALPPDVRHVVIASLPPDRERFAEALGPAGFDQRPVTVTVLCSPDEI
                     PLRRSLLDARAPDRSLLASIYRVLRQWRGEESFWWPDDATWTHLGGALPDLTPSTVDA
                     AVAIFEEAGLAARETIGERNEIQLLAASRRDLTASLRYREGRRDRAAFEECARWMFRA
                     TGIQLLQAAAGGAADARVGQA"
     gene            6446..>7130
                     /locus_tag="VEW91_08940"
     CDS             6446..>7130
                     /locus_tag="VEW91_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYM91744.1"
                     /translation="MKRFHLIRGTPEETLPPDAAALMARVRELTPNADLDLIRRAYLF
                     AREAHADQTRASGEPYVSHSVAVAGILAEMRLDAVTVAAALLHDVPEDTPRTLEEIQA
                     KFSPEVAGLVDGVTKLGRIEWKSREERQAENLRKMFLAMANDIRIILIKLADRVHNLR
                     TIEYLPEWKQKRTAGETLEIYAPLTERLGIGSIKRELEDRAFAVLEPDASREISGQLE
                     RSRHEQEALL"
BASE COUNT          974 a         2524 c         2576 g         1056 t
ORIGIN      
        1 gaggtcgtgc cgcgggggaa cgggacgtta ccctcgccat ggcgcgagcg cgacctttga
       61 tcgtttgaca ctccgcccgg ctggccgata taatgaagcg cggctgggcg ccgtttttta
      121 ttgtctcccg gagggatcgc gcacgtgagg gtcggcaact ccgttcggct gctcgcggtt
      181 ctcctcatcg tggtggcggc gttttggacc gcgttcgagc cgatcgtgtt tccgcgcggg
      241 ctggcgaccc cgacgtttca ggccccagcg ctgcacctca acctcggatt ggacctgcgg
      301 ggcggcagcc acatcgtcct ccaggcgcgg cctacgaaag acaccccggc catcaccaac
      361 gacgcgatgg acggggtcct ccgggtcatc cgcaaccggg tggatcagct cggcgtggcc
      421 gaaccttcga tcacgcgcca gggccgcgac cggatcgtcg ccgaactccc cgggatcgaa
      481 aacccccagc gcgcgatcga actgatcggc aaaacggcgc ttctcgagtt cgtcgacacc
      541 cagacccagt cgctccccca gggggcgcag tggacggaca acgacaccgt cgtcatcccc
      601 gacaacaaga ccacccgcaa gattcccaag aaggtcatcc tgaccggcgc ggatctcgtc
      661 gacgcgcggg gacagttcga ccagttcggc cgccccaacg tcgccttcac cttcaagagc
      721 cgcgcggcca agacgtttga ggactacacc acgaagaaca tcggccgcta cctcacgatc
      781 gtcctggaca gccgggtgat cagctcgccc gtgatcaaca gcccgatccc cggcgggcac
      841 ggggtgatcg agggcgggtt caccctggag tccgcccgcg acctcgcggt gctgctgcgc
      901 ggcggcgcgc tcccgctgcc ggtggaggtg gtcgagaacc gcacgatcgg ccccctgttg
      961 gggcgggact cgatcgatct gagcgtccgg gccggctaca tcgcgatcgt gctggtcgcc
     1021 ctgttcatgg ggctgtacta cgggctcgcc gggatcctgg cggacgtcgc gctcggggtc
     1081 tacacgctca tgctgttcgc ggtgctgacg ctgatcggcg cgacgctgac cctgccgggc
     1141 atcgccgggt tcatcctctc cctcggggtt gcggtggatg ccaacgtgat catcttcgag
     1201 aaggtgaagg aagagctgag ggccggcaag accctccggg ccgccgtcgg cacgggatgg
     1261 gcccgggcga tcgtgaccat cctggactcg aacgccacca cgctgatcgg ggcgctggtg
     1321 ctcctctggc tggggacggg gccggtccgc gggtttgccg tcaccctcat cctgggcatc
     1381 ctgacgagca tcttcacggc gatcgtggtg acgcgggtgt tcgtggacac gacgctcgag
     1441 accggggcgg cggggtggat ccagcgcctc ggggccgtgg gcacccttcg gcgcgccgcc
     1501 cgcgcggccg gcggggccgg ggagtaggcg ggtgccgcag caggccttca acctcatcgg
     1561 caggcgccgc tggtggtacg ccctgtcgct cgcggtgatc gtcccgggtc tcattgccct
     1621 gtacagccac cacgtgcacg accaccacgc gctcaactgg ggggtcgatt tcaccggggg
     1681 caactccctc gagctgcgga tcacccagcc gttcaccgtc ggcgagatcc ggcagatcat
     1741 ggggcagttc cagttgagcg atgcgatcat ccagcggtcc ggcgacaccg aggtcttcgt
     1801 ccggacgcgc ccgctcacgc aggcgcagac gaatgcggtc atccaggcgg tgacggtccg
     1861 gtatcccgcc accaagatgc tccgggtgga caccgtgggg cccgagatcg gggtggagtt
     1921 gcgcaacatc gcgatctttg gggtcgcgat cgggatcatc ctgcaggtca tctacatctc
     1981 gtttcgcttc cattcgatcc ggttcgcgat cgcggccgat atcgccctcc tccacgacct
     2041 gctggtcgtg atcggcgcct ttgcgctcac ccagcgggag gtcaacagca gcttcctcgc
     2101 ggtcctcctg acggtggccg ggtactcgat caacgatacg atcgtcatct tcgaccggat
     2161 ccgcgagaac ctggggttgc gaacgcggga gccgttcgag catctggtga accgcagcgt
     2221 cctcgaggcc ctcgtccgct ccatcaacac cgcgatgacc gcggtcctgg cgatcagcgc
     2281 cgtctacctg ttcgggggcg agacgatccg ggacgttgcg ttcggcctgg tggtgagcat
     2341 catcaccggc ggctactcct cgatcttcaa cgccagcccg attctggtgg actggcacaa
     2401 ctggaccgag cggcaccgcc ggccccgaga cgcccaggcc cggaccgcgc ggatcgtgga
     2461 gcgcgctgag cggactccta cggtccccca gaccgggggc gacgatcccg ccccgctcag
     2521 ggccggggcg cccgggatgc ccgacgaagg cggccggcgc cgccggggcg gccggcgccg
     2581 ccggtagcgg gctcggccat gggtctcacc gtgctctggc tgctgctggt cttcatcgcg
     2641 gcgacggtct ttgcgctgag caattccacg accgtgacgg tcaacttctg gcagtggccg
     2701 gtgtacaccg gcccgctcgc gctcgtcgtc atcggggccg gggtgtgcgg cgcgctgctc
     2761 acctacctgg gctcgctcgt ccaccacatg cgccaggcgc aacagatccg gagcctgcgg
     2821 aagagcatcc aggcgcgcga gacgccgatg gcctcgcctc cgcccggccg caccgaggag
     2881 acccacaggc cgttctgacc gcgccccccg cgcggctgct ttgctgccag cgttataata
     2941 gtaagatgag cgcctctcca tggatcgtcg cgccacccga gcgaggtgcg gcggagctcg
     3001 ccgccgtcct cggggtgcgc ccgctcaccg ccgcgctact gcggcggcgg gggctctcca
     3061 ccgttgaaga cgcccgtcgg tttctcaccc cctgcctcga ggatctggcg aacccgcggt
     3121 ccctccccgg gatggaccgg gcggtggaac gcgtggcgca ggccgtccag gccggcggca
     3181 ggctggccgt acacggcgac tacgacgtcg acgggatctc ggcgaccgcg atcctggtgc
     3241 gcggcctccg cttgctcggc gccgagcccc tctggtacct gccccaccgc ttccacgacg
     3301 ggtacgggct cggcacccgc gcggtcgaga tgtttgccgc acggggggcg accctcgtga
     3361 tcgcggccga ttgcgggatc acggcccacg aggcggtcgc ccgcgcgcgg gcgcttcgga
     3421 tggacgccgt catcctggat catcacgccc ccaccgagga acgccccgcg gccatcatcg
     3481 tggagccgtc cgcgccgctg tgcgccgccg ggatggcgtt catgttcgtg ctggcgctcc
     3541 gggagcggct cgggatcgcg gacgccgtcc ccgcggggat ggcctccctg gcggcgctcg
     3601 gcacgatcgc ggacgtcgtc ccgctgctgg acgacaaccg ccggttggct gcggcggggc
     3661 tggccgagat gcgcgccgcc ccattgccgg gaatcaaggc gctcgccgag atcgcaggcc
     3721 tcaccggccc gatcggcacc tggcacgtcg cgtggcatct ggcgccgcgc ctgaacgcgc
     3781 cggggcggct cggcgacccc tcaccgagcc tgcgcctgct gctcaccgac gatccgggcg
     3841 aggggcgggt cctggcccaa gcgctcgatc aagccaaccg cgagcggcag gagatcctcg
     3901 accggaccct caccgaggcg gtcgcgcagg ccgagcagga ggccgccggg gcggcgctgg
     3961 tcgtggcggg agagggatgg cacccgggcg ttgtgggatt ggtcgccggc cggcttgcgg
     4021 agcagtatcg ccgcccggtc gtggcgatcg gactcgaggg ggacgcgggc cggggctcgg
     4081 ggcggagcat cccggggttc aacctcgtcg gggccctcca gggatgccgg ggctctctgc
     4141 gcgggttcgg gggacacgcc atggccgcgg gcctggtgat cgcgcgggag gcggtggccg
     4201 agttccggag agccttcacc gcgctcgccg cggtgggcgc agcggagcgc gcccggacca
     4261 ccgggctggt cgtcgaggcg gaggtcgggc tcgacgatct gaccgcggcc tgggtggacg
     4321 agctggagcg gctcgcgccg tttggcgccg ccaacccgga gcccgtcctc gccgtgcgcc
     4381 gggtccgggc cctcaaccgc cgggtcatcg gggacggacg gcacctccgg ctcgacgtct
     4441 cggatggtgc cgcaagcatc gaggcgatcg ggttcgcgat ggccgggccc gccgagctgc
     4501 tgacattcac cgaagccccc gtcgatctcg cggttgtccc ggagcgcgat ccattcagcc
     4561 ccgaccgggt tcgactgcgc gtggtggcgc tcgagatccc gggggcggat ccggagcata
     4621 tcctcgcgga tacgggcgcg ctggtggacc ggttgttcag ccgcgccgcg gactacctcg
     4681 gggaggttcg gtacgacggg gtcgaagacg ctccggcgct gcacaccaag gtggtcgggg
     4741 tgacgttcga cggccgccag caggtcctcg ccaccctcca cccgggatcc agcctgaggc
     4801 tcgtccgcga gccggccaac ccccacgatc cccacgcggt ccaggtcgcc acgctggacg
     4861 gccgggtgct gggctacctg cgggccccgc tcgcgggccg gctgtccccc tcgatcgacg
     4921 cgggggcgcg gtatcgggtg accgtcgcgg cggtgacggg cgggggggag cgcaccctgg
     4981 gcgttaacat ctttctcgag cgcgaagacg acgcggacgc atcgcgtgag cacgcccccg
     5041 cacgccggcg ctgggacgcc ggggacaccg agcggctggc cgccgcgctc aacggcggcc
     5101 agccgctggc ccctgtgcac gccgaggtgc tcagggcggc cgcctccggg cggcccatgg
     5161 tcctgaactt tccccccggg cgggggccag cgacggcact cgccggcgtc gcggcgctcg
     5221 gggcgcgcga gggccggtgc acgctcgtgg tcgctccgct gcgccggcag gtgctgcacc
     5281 gcgcagacca gctgcgctcg cgcctggctc ccctcgggct ccgggtgcgc gccgtccacg
     5341 ggttgcaggg atcccgggag cgggcgcgcg tggacgccgc attgcgcgcc ggcggggtgg
     5401 acgtcctggt cgccagcgcc gaagccttgg ccgccggatg gcccgaggcg catgcggacc
     5461 gggtcggcac cctggtgtgc gacggcgtcg gtggggcaga ccttccgccc ctccttcgcg
     5521 cgttcgccgg cgcgcaggtg tgcctgatga acgtcccggc ccccgccgcg gccggagaat
     5581 ccttggggcc cggcgcggtc gtgattcgcg atgcaggcgt gcggatcggg ctgcggatgg
     5641 tcgatcgcag gagcgccgcc gacagggacg ccgtggtaga ggaggtcctg cggggcggcg
     5701 agaaaaccgt agttcatgcc ggcgggcgcg aagcgtgcgt gcagttggcg gtgcgattgc
     5761 gtgaccggtg cggccagcac gcacggctcg gttacctgca cgagggcctg cccgcgcgcg
     5821 tgcgccagat catcgtccag gcgttccgcg agggccgcct ggggatcctg gtgaccacgc
     5881 cggccctcga cgaggaagcg ctcccccccg acgtccgcca cgtcgtgatc gcgtccctcc
     5941 ccccggaccg cgagcgattc gccgaagcgc tggggcccgc agggttcgac cagcgcccgg
     6001 tgaccgtcac cgtgctgtgc tccccggacg agattccgct ccggcggtcc ctgctggacg
     6061 cccgcgcccc ggaccggtcg ctcctcgcgt cgatctaccg cgtcctccgg cagtggcgcg
     6121 gggaggagtc gttctggtgg cccgacgacg ccacgtggac ccacctgggc ggggccctgc
     6181 ccgatctcac accgtcgacc gtcgatgccg cggtggcgat cttcgaggaa gccgggctgg
     6241 cggcccggga gacgatcggg gagcggaacg agatccagct cctcgccgcc tctcgccggg
     6301 atctcaccgc atcgctccgg taccgggagg gccggcggga tcgcgccgca ttcgaggagt
     6361 gcgcccggtg gatgttccgc gccaccggca tccagctgct ccaggcggcc gccggtgggg
     6421 cggccgacgc gcgggtgggg caggcatgaa gcggttccac ctgatccggg gaactccgga
     6481 ggaaacgctt cctcccgacg ccgccgccct gatggcccgc gtcagggagc tgaccccaaa
     6541 cgcggacctc gacctcatcc ggcgggcgta cctgttcgcc cgggaggccc acgccgatca
     6601 gacgcgggcg tccggtgagc cctacgtcag ccacagcgtg gcggtggccg ggatcctggc
     6661 cgagatgcgc ctggacgcgg tcacggtcgc ggcggcgctg ctccacgatg tccccgagga
     6721 caccccgcgc acgctcgagg agatccaggc gaagttcagc cccgaggtcg ccgggctggt
     6781 cgacggcgtc accaagctgg gtcggatcga atggaagagc cgcgaggagc gccaggcgga
     6841 gaacctccgg aagatgttcc tggcgatggc caacgatatc cggatcatcc tgatcaagct
     6901 ggccgaccgc gtgcataatc ttagaacgat cgagtacctc ccggagtgga agcagaagcg
     6961 cacggccggc gagaccctgg agatctacgc gccgctcacc gagcggctgg gcatcgggag
     7021 catcaagcgt gaactggagg accgggcgtt cgccgtgctc gagccggacg cgtcccgcga
     7081 gatcagcgga caactcgagc gcagccggca cgagcaggag gcgttgctcg
//
LOCUS       DASQGH010000454         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_35959, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000454 DASQGH010000000
VERSION     DASQGH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35959"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VEW91_08945"
     CDS             complement(<1..490)
                     /locus_tag="VEW91_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM91745.1"
                     /translation="MGLHRPEPGCPVPTPQGAERARLIERTLEQYRRYLNPGLARLFQ
                     FGGVETVEWAAEGCLVWDVHGREYIDCACGPAIFNIGHRHPRVLAAVRDQLERIPMSV
                     RTMPSAPQAALAERLAQLSPGDLQYAFFCNSGAEANEGAIKLARLATGRPGIVAMQGA
                     FHG"
     gene            complement(471..968)
                     /locus_tag="VEW91_08950"
     CDS             complement(471..968)
                     /locus_tag="VEW91_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYM91746.1"
                     /translation="MRRAHSAGGVVFRRPQPDTPGAAVQILLLQHEAGKWMLPKGTIE
                     AGETPEAVALREVAEETGLRNVRIVQDLGEERYLFFWKTEDTYYDKTVHYYLMEFLGG
                     EETRPQREEGFVRCDWVSVPEALERIRYKETREVVRRAEAALAAAASRESSAAPEVRH
                     GSASP"
     gene            complement(1202..1369)
                     /gene="rpsU"
                     /locus_tag="VEW91_08955"
     CDS             complement(1202..1369)
                     /gene="rpsU"
                     /locus_tag="VEW91_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HYM91747.1"
                     /translation="MTEVRVGKDETLDSALRRFKRQVKRSGILTDAKRHEHYEKPSQK
                     RRRRAARRKRF"
     gene            complement(1485..1916)
                     /locus_tag="VEW91_08960"
     CDS             complement(1485..1916)
                     /locus_tag="VEW91_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYM91748.1"
                     /translation="MRNVSPQRGFTMLELLIAVAVAAILVTLTYTNWRGYSAQQRLRY
                     AAVQVASGLREAEERAKAERTIYTVTFTSSSATYTILRTSGGFIDNAVLPSGITPQAS
                     DTVTFSAFGVPDAAHTITLQGTAGTRTASVDIVGGITYQGP"
     gene            complement(1927..>2217)
                     /locus_tag="VEW91_08965"
     CDS             complement(1927..>2217)
                     /locus_tag="VEW91_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4900 domain-containing protein"
                     /protein_id="HYM91749.1"
                     /translation="SSYNSSVNVQNYNSGSPRGNVNLLGGLIEKYYGAFGTFNPSTGQ
                     NLTGYGRAFTYDTRMSRGFTPPFFPTTNLYVVTDGSQPLAGVKATWREGTPP"
BASE COUNT          314 a          801 c          738 g          364 t
ORIGIN      
        1 tgccgtggaa cgccccctgc atcgcgacga tgcccggccg gccggtcgcc agcctcgcca
       61 gtttgatcgc gccctcgttc gcctcggctc cgctgttaca gaagaacgcg tactggagat
      121 cgcccgggga aagctgggcc aaccgctcgg cgagggccgc ctgcggcgca ctcggcatcg
      181 tgcggaccga catcgggatc cgctcgagtt ggtcgcgcac cgccgcgagg acccgcgggt
      241 ggcgatgccc gatgttgaag atcgccgggc cgcacgcgca gtcgatgtac tcccggccgt
      301 ggacgtccca gaccaggcag ccttcggccg cccactccac cgtctccacg ccgccgaact
      361 ggaacaggcg ggccagcccg gggttgaggt accgccggta ctgctccagg gtgcgctcga
      421 tcaaccgggc ccgctcggcg ccctgaggcg ttggcaccgg gcagccgggt tcagggcgat
      481 gcagacccat gccggacctc cggcgccgcg ctcgactctc gggaggccgc cgcggcgagc
      541 gccgcctcgg cccgccgcac gacctctcgg gtctccttgt acctgatccg ctccagggcc
      601 tcggggaccg acacccagtc gcaccggacg aacccttctt cacgctgcgg gcgtgtttct
      661 tccccgccga ggaactccat caagtagtag tgcaccgtct tgtcatagta cgtgtcctcg
      721 gtcttccaga agaacaggta ccgctcttcc cccaggtcct ggacgattcg gacgttgcgc
      781 agcccggtct cctcggccac ctccctgagc gcaacggcct cgggggtctc gcccgcctcg
      841 atcgtcccct tcgggagcat ccacttcccc gcttcgtgct gcaagaggag gatctggaca
      901 gcggcgcccg gggtgtcagg ctggggccgc cggaacacca ccccgccggc gctgtgggcc
      961 cgtcgcatcc ttcgggccgg ctgccgcacg ctcacgctcc cctcgcccga tcgggcgcaa
     1021 ctgggtcaag ggccggcggc tccgaaggtc cccgagccgc cggcccatcc gacagtcgat
     1081 ccacccgtac tcgacgaagc gccagcatcg tccatggctc cccgccgtct ccggctgcca
     1141 cgactccccc gcgccccccg gcggggcccc gagatgcgat ctgcggaagc cggccggaag
     1201 cctagaagcg tttgcgccgg gccgcgcggc ggcgccgctt ctggctcggc ttctcgtagt
     1261 gctcgtgccg cttcgcgtct gtgaggatgc cggaccgctt gacctgccgc ttgaaacgcc
     1321 gaagcgcgct gtccagcgtc tcgtcctttc cgacccgcac ttcggtcaac ggcgcacccc
     1381 cggctgcccg gctgcgaagg atgaacccat tatacacgag gagcgcccac atcatcaacg
     1441 ggcgctcctg cgagcgtgcg gacgtgcgtg cggggacgag tgcgctacgg gccctggtac
     1501 gtgatccccc cgacgatgtc cacggacgcc gtccgggtgc ctgcggtgcc ctgcagcgtg
     1561 atcgtgtgcg cggcatcggg caccccaaag gcagagaacg tcaccgtgtc gctcgcctgg
     1621 ggtgtaatgc cgctcggcag cacggcattg tcgatgaagc cgccggaggt gcgaaggatg
     1681 gtgtaggtcg ccgaggacga ggtgaacgtc acggtgtaga tcgtgcgctc ggccttcgcg
     1741 cgctcctcgg cttcccggag cccgctggcc acctgcacgg cggcgtaccg cagccgctgt
     1801 tgggcgctgt agccgcgcca gttagtatag gtcagagtca caagaatcgc tgccacggcc
     1861 acggcgatga gcagctccag catggtaaac ccgcgttggg gggagacgtt cctcatggcc
     1921 cggcccctac ggtggggtcc cctcacgcca cgtcgccttg acccctgcga ggggttgcga
     1981 gccgtccgtg acgacgtaga ggttggtggt cgggaagaac ggcggcgtaa acccccggct
     2041 catccgcgtg tcgtaggtga aggcccgccc gtagccggtg aggttctgcc ccgtggaggg
     2101 gttgaacgtc ccgaaggccc cgtagtactt ctcgatcagg ccgccgagga ggttgacgtt
     2161 cccccgcggg ctgccgctgt tgtagttctg cacgttgacc gagctgttgt agctgct
//
LOCUS       DASQGH010000455         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4877, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000455 DASQGH010000000
VERSION     DASQGH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4877"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VEW91_08970"
     CDS             complement(<1..318)
                     /locus_tag="VEW91_08970"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYM91750.1"
                     /translation="MARVEGAVLIRAPLDRVYTLAKRVEDFPTFMPDLERITVLERHA
                     GVPAVTEWVGIVEGRRIRWVEEDAWDDARHLCRFRQREGDFERYEGTWTFAPEAEGTR
                     TTIV"
     gene            complement(340..1311)
                     /locus_tag="VEW91_08975"
     CDS             complement(340..1311)
                     /locus_tag="VEW91_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYM91751.1"
                     /translation="MPANLTPQYLEAERRFREATTPDEQLKALDEMMATIPKHKGTER
                     MRADIRRRMAKLRTEAARKRSVGRGSTWQHVPREGAGQIVLVGPPNAGKSRLLAALSH
                     ATPVVAPYPFATRTPLPGMVGFENVQIQLVDLPPIAPETAEPWLFALIRQADAALLVA
                     DLESDDLLSALEETLDLAGRSNVRLARHGAPDAGVPTLLVAAKADAPGAADRLEILRE
                     VFASRFPILAVSAEAGMNLDELRKEMFQLLNVIRVYTKIPGRRADLSAPFVLKRGTTV
                     QDAAGIVHKDFTDRLKYARIWGSRTFDGQMVQHEHILEDGDILELHA"
     gene            complement(1386..2252)
                     /gene="rfbD"
                     /locus_tag="VEW91_08980"
     CDS             complement(1386..2252)
                     /gene="rfbD"
                     /locus_tag="VEW91_08980"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HYM91752.1"
                     /translation="MKVAVIGANGQLGTDLVRALGKAGGFETRPLMHDEIEVADPGSV
                     RTTLTQARPEVVVNCAAFNRVDECEDRPEEAMRVNALGALHVARACADLGALCVYVST
                     DYVFSGEQRRPYVESDAAGPINVYGASKLAGEQLTVGASPRWLIVRTASLFGTTPARV
                     KGGNFVETMLAKARAGEAIRVVADIRMSPTYAADAASGLARLIRQGVGGTVHLTNAGS
                     CTWYEFARQILTAAGLEANVVPVGSSEYPTKARRPKDSSLASLRADDGVRRCLRPWGE
                     ALSAYLADRTMR"
     gene            complement(2269..2829)
                     /gene="rfbC"
                     /locus_tag="VEW91_08985"
     CDS             complement(2269..2829)
                     /gene="rfbC"
                     /locus_tag="VEW91_08985"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HYM91753.1"
                     /translation="MPFRFARLDIPEVVLIEARPYPDARGFLMETYRRSLFEGAGIPQ
                     AFVQENYSRSQKGVLRGLHYQKLPQAQGKLISVVRGEVFDVAVDLRRGSPTYGRWVSV
                     TLSSETQRLLYVPVGFAHRFCATSDEADVVYKVTAEYAPDLEGGIIWNDPELRIPWPI
                     REPLLSPRDAQLGRLRDADHNFVYQR"
BASE COUNT          412 a         1011 c         1028 g          456 t
ORIGIN      
        1 gacgatcgtg gtccgcgttc cctcggcttc cggggcgaac gtccaggtgc cctcgtagcg
       61 ctcgaagtcg ccctcgcgct ggcggaaccg gcagaggtgc cgggcatcgt cccaggcgtc
      121 ctcttcgacc caccggatcc ggcggccttc cacgatcccc acccactccg tcacggcggg
      181 cacccccgcg tgccgctcca gcacggtgat ccgctccaga tcgggcatga acgtggggaa
      241 gtcctccacg cgcttcgcaa gggtgtagac gcggtcgagc ggggcgcgaa tcaacacggc
      301 cccttccacg cgggccatac tggtcacctc ctcggggggc tacgcgtgca gttcgaggat
      361 gtccccgtcc tccaggatgt gctcgtgctg caccatctgg ccgtcgaagg tgcgcgagcc
      421 ccagatccgc gcatacttga gccgatcggt aaagtccttg tgcacgatcc ctgcggcgtc
      481 ctgcacggtc gtcccgcgct tgaggacgaa cggggcggac aggtccgcgc ggcggccggg
      541 gatcttggtg tacacgcgga tcacgttcag cagctggaac atctccttgc gcagttcgtc
      601 caggttcatc ccggcctcgg cggagaccgc cagaatcggg aaccggctgg cgaacacctc
      661 gcggaggatc tcgaggcgat ccgccgcccc cggcgcgtcc gccttggcgg cgaccagcag
      721 cgtgggcacg ccggcgtctg gggcaccgtg gcgcgccagc cgcacgttcg accggcccgc
      781 gaggtcgagc gtttcctcca gggcggagag gagatcgtcg ctctcgaggt cggccacgag
      841 cagcgcggcg tcggcctgcc ggatcagcgc gaacagccag ggttccgcgg tttccggggc
      901 gatcggcgga aggtcgacga gctggatctg cacgttctcg aagcccacca tgccggggag
      961 cggcgtgcgg gtcgcaaacg gatagggggc gacgaccggc gtggcgtggc tgagggccgc
     1021 gaggaggcgc gacttgcccg cgttgggggg ccccaccagg acgatctgcc cggccccttc
     1081 gcggggcacg tgctgccagg tcgatccgcg ccccaccgac cgcttgcggg ccgcctcggt
     1141 gcggagcttc gccatccgcc gccggatgtc cgcccgcatg cgctcggtcc ccttgtgctt
     1201 ggggatcgtg gccatcatct cgtcgagcgc cttgagctgc tcgtcggggg ttgtggcctc
     1261 gcggaaccgc cgctcggcct cgaggtattg gggagtcagg ttcgcgggca tgcaggatcc
     1321 ggcctcacgc ctcattatat ggcacgagca gggagcggac caggagcggg gcgggcgtgg
     1381 gttcgctagc gcatcgtgcg gtcggcgagg taggccgaaa gcgcttcgcc ccacgggcgg
     1441 aggcagcggc ggacgccgtc atccgcgcgg aggctggcga gcgaggagtc tttggggcgc
     1501 cgggctttcg tgggatactc cgaggacccc accggcacga cgttggcctc gaggcccgca
     1561 gcggtgagga tctgcctggc gaactcatac caggtgcacg acccggcgtt agtcaggtgg
     1621 acggtccctc ccaccccctg ccggatgagt cgcgcgagcc cgcttgcggc gtcagccgcg
     1681 taggtcggcg acatccgaat gtcggcgacc acccggatgg cttcgcccgc ccgcgccttg
     1741 gccagcatcg tttccacgaa gttgccgccc ttgacgcgcg cgggtgtcgt gccgaagagg
     1801 ctggcggtgc ggacgatgag ccagcggggg gaggccccca ccgtcagctg ctcgccggcg
     1861 agcttggagg caccgtagac gttgatgggc cccgcggcat ctgattcgac gtacggccgg
     1921 cgctgctcgc cgctgaatac gtagtccgtg ctcacgtaga cgcacagcgc gcccaggtcc
     1981 gcgcaggccc gcgcgacatg gagggcgccg agggcgttca cccgcatcgc ctcctcgggc
     2041 cggtcctcgc actcgtcgac ccggttgaag gcggcgcagt tcaccacgac ctcggggcgg
     2101 gcctgggtca gggtggtgcg caccgacccc gggtccgcca cctcgatctc gtcgtgcatg
     2161 agcgggcggg tctcgaagcc gcccgccttg cccaacgccc gcacgaggtc cgttcccaac
     2221 tggccgttgg cgccgatgac ggcgactttc attggcggat cgggcgcctc accgttggta
     2281 cacgaagttg tggtcggcgt cgcgcaggcg ccccaattgc gcgtcccgcg gggagaggag
     2341 gggctcccgg atcggccaag ggatcctgag ctcggggtcg ttccagatga tgccgccctc
     2401 caggtcggga gcgtattcgg cggtgacctt gtagaccacg tcggcctcgt cgctggtggc
     2461 gcagaacctg tgggcaaagc cgaccgggac gtacagcagg cgctgtgtct cgctggacag
     2521 ggtcacactg acccaccggc cgtaggtcgg cgagccccgc cggaggtcga ccgcgacatc
     2581 gaagacctct ccccgcacca cgctgatcag cttcccctgg gcctggggga gcttctggta
     2641 gtggaggccg cgcagcacgc ccttttgcga ccgcgagtag ttctcctgga caaacgcctg
     2701 cgggatgccg gcgccctcga acaaggatcg ccggtacgtt tccatgagga atccccgggc
     2761 atccggatac ggccgggctt cgatcagcac cacctcgggg atgtcgagcc ttgcaaaccg
     2821 gaacggcatc ggccggcgcc ctccccctcc gctcttcgcc tgcgcgtcct aagatatgcc
     2881 cgtcgccgga ccgcggcaga cgccccc
//
LOCUS       DASQGH010000456         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55651, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000456 DASQGH010000000
VERSION     DASQGH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55651"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VEW91_08990"
     CDS             complement(<1..1018)
                     /locus_tag="VEW91_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HYM91754.1"
                     /translation="MLPPQKVAEIVVRGNDKVPTEQILGAIATKVTDPLNEEKLRNDV
                     QAILNLGVFADAVVRFETVPDGVRVVFLVVENPVVQQIQVKGNTVVSTPDVLKALGIQ
                     TGAVLNTVTMRTGVRAVERLYQDQGYVLAHVTDVNVSPEGTLTLQIAEGRIEAIKIEG
                     LVKTHDYVVRRELTFKPGEVFNVNAVNASLKRLFQLQYFSDVKAQPGPGTTPDAVVVT
                     LVVTEQKTASVSFGVGYGNQTGLEGIIGVREINLFGNGQTVSLQYSNTVLYGTSYVLA
                     FHEPYFLGSRTIMDVQLFNQTSIPTEYQALGPANSFQYDITQVGGLLTFTTPVDPINS
                     LSYGI"
     gene            complement(1145..1360)
                     /locus_tag="VEW91_08995"
     CDS             complement(1145..1360)
                     /locus_tag="VEW91_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91755.1"
                     /translation="MTDPRTRTVGVAAYGLAWSLLLVAATLGGLWAEPAAPGRQRAAQ
                     PPSQPAPPPAQPAPPPSQPAPPPPPPA"
     gene            complement(1392..1880)
                     /locus_tag="VEW91_09000"
     CDS             complement(1392..1880)
                     /locus_tag="VEW91_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91756.1"
                     /translation="MVIPEGMLVHIRHAEGWLRRARADCGRGEARRAVLRLLLAEAEI
                     RRARESEGGTAPHPFARSGRPRWAVLGAVAAVGVVLAAIALVRPFAPSPIATAPGVFR
                     ATPAAQRSASILRFESGRVLPIVGFPTEVRSDGRPAPGFAWSSEDPLLAGDDSPGLVT
                     FH"
     gene            complement(1874..2542)
                     /locus_tag="VEW91_09005"
     CDS             complement(1874..2542)
                     /locus_tag="VEW91_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYM91757.1"
                     /translation="MVERTVLAGYLRELRKVPRLRPEEEARLWRAYKGRGDARSRSAL
                     IESYQPLVFKVAMHLRLREGIVMDMIQEGTVGLIEAAERYDPGRGVRFSTFATYRIRG
                     RILNALHRERPLPAVEGPEGQLAGSLAERVQDVAAADRLARVEDAMVAEQIVAAIERL
                     PEREQRILRAMISREEEPRRIAGELRISLSHLYRLQKRAVQRLQAHLMPSSPGMRAQE
                     KAAW"
     gene            complement(2584..>3721)
                     /locus_tag="VEW91_09010"
     CDS             complement(2584..>3721)
                     /locus_tag="VEW91_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HYM91758.1"
                     /translation="AGTIAVPQMTGFLRTSGGRLRYAPLRTPIEDLRIDVAFSQDQVQ
                     VRDLSAAFGQGRIGADGVVAITNFRPDNVRLALRAEHATLDAPGLYTGQVDAELEITG
                     QASQPTLSGRAVLSNGQVAPGGAEGAGGAPNLDLNVSLEAGPNTVFTLGAVRAQVEGA
                     LHAGGTLGHPVLAGRVTSPEGEVAFLGSTFRLTGGEAVFTEALGLDPQISAQGERVVG
                     NTIVFLDFCGLASELRVCRASSVPPHTEGEIYSLVAADAGISGDPQSVIGQGLGRSLL
                     GSFREALHLSEFTVAYSRESPVTLRMGTFLLQNVYLTLSEVWPGPPGATTLTLGTTPR
                     QQLLTGQPYSVAGVEYFLSPNVLATFNVDTLGGAGVFLLTQFPL"
BASE COUNT          563 a         1296 c         1296 g          566 t
ORIGIN      
        1 tgatcccgta gctgagcgag ttgatgggat cgacgggagt ggtgaaggtg aggagccccc
       61 ccacctgcgt tatgtcgtac tgaaacgagt tggcgggccc aagggcctgg tactccgtgg
      121 gaatcgacgt ctggttgaac agttggacgt ccatgatcgt gcggctcccg agaaaatacg
      181 gctcgtggaa cgcgagcacg tagctggtgc catacaggac cgtgttgctg tactggagcg
      241 agaccgtctg cccgttcccg aagaggttga tctcccgcac cccgatgatt ccttcgaggc
      301 cggtctggtt gccgtacccg accccgaagc tcaccgaggc ggtcttctgt tcggtgacga
      361 cgagcgtgac gaccacggcg tccggggtgg tgccgggccc gggctgcgct ttgacgtcgg
      421 agaagtactg cagttgaaat aggcgcttga ggctcgcgtt gaccgcgttg acgttgaaca
      481 cctcgcccgg tttgaaggtg agctcgcggc gcaccacgta gtcgtgggtc tttaccagcc
      541 cctcgatctt gatcgcctcg atgcgcccct cggcgatttg gagggtgagg gtgccttccg
      601 ggctgacgtt gacgtccgtg acgtgggcca gcacgtaccc ctgatcctgg tacagcctct
      661 ctaccgcgcg aaccccggtc cgcatcgtca cggtattgag caccgcgccc gtctggatcc
      721 caagcgcctt cagcacgtcc ggggtggaca cgacggtgtt tcccttcacc tggatctgct
      781 gcacgacggg gttctccacg acgaggaaca cgacccggac cccgtcgggc acggtttcga
      841 accggaccac cgcgtcggca aacaccccga ggttgaggat cgcctgcaca tcgttgcgga
      901 gtttctcctc gttgagcgga tcggtcacct tggtggcgat cgccccgagg atctgctcgg
      961 tcgggacctt gtcgttcccc cggacgacga tctcggccac cttttgcggt gggagcacct
     1021 gcggggggac gcccggcggc gggacaaagg ggctcggggg gatcagggtc ggcgcgggcg
     1081 gcgccggcgg cggggccggg gctggcgcgg gcggcgatgg gggttgcgcc ggcggcgacg
     1141 ggggttacgc cggcggcggc gggggcggcg cgggctgcga tgggggcggg gccggctgcg
     1201 ccggcggcgg ggccggctgc gatggcggtt gggccgcccg ctgcctccct ggcgcggcag
     1261 gctcagccca caacccgccc agggtcgccg cgacgagcag cagcgaccac gcgaggccgt
     1321 aggcggccac tcccaccgtc cgcgtccgag ggtcggtcat cgtccctcca tcgcaattcg
     1381 aggatcaggc gctaatggaa tgtcacaagt cctggactgt cgtcaccagc gagcagcggg
     1441 tcttcggaac tccacgcgaa tccgggggcc ggcctcccgt cggaccgcac ctcggtggga
     1501 aaccccacga tgggcagcac ccgcccgctc tcgaaccgga ggatgctggc ggatcgctgc
     1561 gccgccgggg tcgcccggaa cacaccgggg gccgtggcga tcggactcgg cgcgaacggc
     1621 ctgaccagcg cgattgcggc gaggactacg cccaccgccg cgactgcccc aaggaccgcc
     1681 caccgggggc ggccggaccg cgcgaacgga tggggggctg tccctccctc cgactcccgg
     1741 gctcggcgga tctccgcctc ggccaggagc agccgcagca ccgcccgccg cgcttccccc
     1801 cggccgcagt cggccctggc ccggcggagc cacccttcgg cgtggcggat atggacgagc
     1861 atcccctcgg gaatcaccac gccgctttct cctgcgcccg catgccaggc gaggacggca
     1921 tcagatgcgc ctggagccgt tgcaccgccc gcttctggag gcggtacaga tgcgagagac
     1981 tgattcgaag ttcgccggcg atccgcctcg gctcctcctc cctcgagatc atcgcccgca
     2041 ggatgcgctg ctcgcgctcc ggcagccgct cgatcgcggc cacaatttgc tcggcaacca
     2101 tcgcgtcctc aacgcgcgcc aggcggtccg ccgcggcgac gtcctggacg cgctcggcca
     2161 gcgatcctgc gagctgcccc tcggggccct cgactgccgg gagcggtcgc tcgcgatgga
     2221 gggcattgag gatgcggccc cggatccggt aggtggcgaa ggtgctgaac cgcacgcccc
     2281 ggccgggatc gtagcgctcc gcggcctcga tgagcccaac cgtgccctcc tggatcatgt
     2341 ccatgacgat cccctcgcgg agccgcaggt gcatcgccac cttgaacacg agcggctgat
     2401 acgactcgat gagggcgcta cggctccgcg cgtcgccgcg gcccttgtac gcgcgccaca
     2461 gccgcgcttc ctcctccgga cgaagccggg gcaccttacg cagttcgcgt agatacccgg
     2521 caagcaccgt gcgctccacc atcaaagaat atccatggtg tcgagggagc gttctcctcc
     2581 ggatcagagc ggaaactggg tcagcagaaa taccccggcc ccgcccagcg tgtccacgtt
     2641 gaacgtggcc aggacgttgg ggctgaggaa gtactccacc ccggcgaccg agtagggctg
     2701 cccggtcagg agctgctgcc tgggcgtcgt gccaagggtg agggtggtgg ccccgggcgg
     2761 ccccggccac acctcggaca gcgtcaggta cacgttctgg aggaggaacg tgcccatgcg
     2821 gagcgtgacg gggctctcgc ggctgtaggc gaccgtgaac tcgctcaggt gcagggcctc
     2881 gcggaacgac cccagcaatg agcgccccag cccctggccg atcaccgact gggggtctcc
     2941 ggagatcccg gcgtcggccg ccacgagcga gtagatctca ccctcggtgt gcggcgggac
     3001 cgatgaggcg cgacacaccc gaagttcact ggccagcccg cagaagtcga gaaacacgat
     3061 ggtgttgccg acgacccgct ccccttgcgc ggagatctgc ggatccaggc cgagggcctc
     3121 tgtgaacacg gcctctcccc cggtcagcct gaacgtcgat cccaggaagg cgacctcgcc
     3181 ctcgggggag gtgacgcggc cggcgagcac cgggtgaccg agggtgccgc cggcgtggag
     3241 cgcgccttcg acctgcgccc ggaccgcgcc cagggtgaac acggtgttgg gacccgcttc
     3301 caggctcacg ttgaggtcga ggttgggcgc gccccctgcg ccctcagccc ccccgggggc
     3361 aacctgcccg ttcgaaagga ccgcccggcc cgacagcgtg ggctgactcg cctgccccgt
     3421 gatctccaac tccgcgtcca cctggccggt gtagagcccc ggcgcatcga gggtggcgtg
     3481 ctcggcccgg agcgccagcc ggacgttgtc cggacgaaaa ttcgtgatcg ccaccacccc
     3541 gtccgctccg atccgtccct gcccgaaggc ggccgagaga tcccgcacct ggacctggtc
     3601 ctgggaaaag gccacatcga tgcgcaggtc ctcgatcggg gtgcgcaggg gcgcgtagcg
     3661 caaccgtccg ccgctagtcc gcaggaaccc ggtcatctgg gggaccgcga tcgtgcccgc
     3721 c
//
LOCUS       DASQGH010000457         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33225, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000457 DASQGH010000000
VERSION     DASQGH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33225"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VEW91_09015"
     CDS             complement(<1..604)
                     /locus_tag="VEW91_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYM91759.1"
                     /translation="MSSALVLKQTPLFAQLSEGHLARLAALARTQRYGRERVIFNEGD
                     PGTALYVIVKGRVKISKGSPDGKERTLGLLGPGTVFGELAVLDGGPRSAEAVVLEDAE
                     LLAVPREAFLAFLLEHPQAAMSLLVVLSQRLRRTDQLVSDAAFFDVRGRLARVLLELA
                     QAQGQRESDGAFACPQLTQTELASMVGVTRESINKWLRYYE"
     gene            complement(713..1597)
                     /locus_tag="VEW91_09020"
     CDS             complement(713..1597)
                     /locus_tag="VEW91_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91760.1"
                     /translation="MRTSGNGRTPNRLTARADPRLHDQLRRLARDQRMVFFAGLPGTG
                     KSLLVHQLAHLAEALGRTVHLLQWDVARPVFEASVAGRRYPIVGGVTHNVIRKAIGAW
                     VRDAVVRWDRQHPDPAHLLVGETPLVGHRFIELVRRLGDAAEPLLAATSTRFAICVPS
                     RRLRRFLEEERERRTVRPTHRQELEDARSSVLRDLWRQLVAVAPSLGLSPAPDAPYDP
                     TLYQHVYERLLCHRHTEVVSLATRLPAGARSVHDFAIPRRDLVPDPDETDGFIREIET
                     RYPDPEALQREVDRWYLV"
     gene            complement(1616..2221)
                     /locus_tag="VEW91_09025"
     CDS             complement(1616..2221)
                     /locus_tag="VEW91_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91761.1"
                     /translation="MKDGPRRWRRAGAVLAAAWLLAALLPQASGQPEYSLYYHFEIPL
                     TVLGLTVTDDGTKRTYKGTLLGTLGGIPVTDSRYTYANGATAQAGGGTFSMTTRAGSV
                     RDGQVLMTSDGTQTTLLFLGTYLGTDLSFSIVGTGDQIGGSGVTADGLAETTFRSHEH
                     YIAAVQEATTAFPQAMRTQIIAQASQNPRLVREYQQRSTPR"
     gene            2415..>2671
                     /locus_tag="VEW91_09030"
     CDS             2415..>2671
                     /locus_tag="VEW91_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HYM91762.1"
                     /translation="MAFVDKTLHCLDCGKEFVFTAGEQEFYAKKGFTNEPLRCKECRD
                     ARKRSREGAAGMPREMFDAVCAKCGAKTQVPFRPREDRPVY"
BASE COUNT          420 a          862 c          951 g          438 t
ORIGIN      
        1 gttcgtagta tcggagccac ttgttgatgc tttcgcgggt gacgccaacc atgctggcga
       61 gctccgtctg ggtgagctgg gggcacgcga acgcgccgtc ggactctctc tggccctggg
      121 cctgcgcgag ctccagcagc acccgggcca gcctgccccg aacgtcaaag aacgcggcat
      181 cggacaccag ctggtcggta cgtctcagcc gttggctcaa caccaccagc aggctcatcg
      241 ccgcctgcgg gtgctccaga agaaaggcaa ggaacgcttc gcgggggacc gccagcaatt
      301 cggcgtcctc caggacgacc gcttccgccg accgcggccc accgtcgagt acggccagct
      361 cgccgaagac agttccggga ccgagcaggc ccagggtccg ttccttgccg tctggagaac
      421 ccttggagat cttgacccgc cccttcacga tgacatacag ggcggtgccc ggatcccctt
      481 cgttgaagat tacacgctct ctcccgtagc gctgcgttcg ggcgagcgca gccaagcggg
      541 cgaggtgacc ttcggacagt tgggcgaaca gcggggtctg cttcaagacc agggcgctcg
      601 acatctccga ccgcctccgg tgtcctcccc cagcatacac cctcagggcc cgggtgtgaa
      661 ccgccggccg tctcgcgcgc ccggaccctg cgctgcgcag gacgaccggc agtcagacca
      721 gataccagcg gtcgacctcg cgctgcagcg cttcggggtc ggggtaccgg gtttcgatct
      781 cccggatgaa gccgtcggtc tcgtcgggat cgggcacgag atcgcgccgg gggatggcga
      841 agtcgtggac ggagcgggcg cctgccggca ggcgggtcgc gagggacacg acctccgtgt
      901 ggcggtggca cagcagtcgc tcgtagacgt gctgatacag cgttggatcg tagggggcgt
      961 ccggggccgg cgagaggccg agcgacggtg ccacggcgac gagctgccgc cagaggtcgc
     1021 gcagcaccga ggaccgggcg tcctccagct cctgccggtg cgtcgggcgc acggtgcggc
     1081 gttcccgctc ttcctcgagg aaccgccgta atctgcgaga cggcacgcag atggcgaatc
     1141 gcgtggaggt cgcagcgagg agcggctccg cggcatcgcc gaggcggcgc acgagctcga
     1201 tgaagcggtg gccgacgagc ggtgtttcgc cgacgagcaa gtgggcggga tcgggatgct
     1261 gtcgatccca gcgcacgacg gcgtcgcgga cccacgcgcc gatggccttc cggatcacat
     1321 tatgcgtgac cccacccacg atgggatagc gccgtccggc cacgctggct tcgaagacgg
     1381 gccgcgcgac gtcccactgc agcaggtgca cggttcgccc cagcgcctct gccaggtgcg
     1441 cgagctgatg gaccagcagg ctcttgccgg tgccgggcaa cccggcgaag aacaccatgc
     1501 gctggtcgcg cgcgagccgc cggagctggt cgtggaggcg cggatccgcg cgcgcggtga
     1561 ggcgattggg ggtcctcccg tttccgcttg tgcgcaccgg cggggggcag tgcgatcagc
     1621 gcggggtgga ccgttgctgg tactctctga ccagccgggg attctggctg gcctgggcga
     1681 tgatctgggt gcgcatggcc tgggggaacg ccgtggtcgc ctcttggacg gcggcgatgt
     1741 agtgttcatg agagcggaag gtcgtttcgg cgaggccgtc ggcggtcacc ccgctcccgc
     1801 cgatctggtc acccgtgccc acgatggaaa atgataggtc cgttccgagg tacgtcccga
     1861 ggaacagcag ggtcgtttgc gtcccatcgc tcgtcatcag gacctgccca tcccggaccg
     1921 atcccgccct ggtggtcatg gaaaacgtcc cgccacctgc ctgcgcggtg gcgccgtttg
     1981 cgtaggtata ccgggagtcg gtgaccggga tccctcccag ggtgcccagg agggtcccct
     2041 tgtacgtgcg cttcgtcccg tcgtccgtca ccgtgagccc caggacggtc aacgggatct
     2101 cgaagtggta gtagagcgaa tactcgggtt gcccggaggc ctggggcagt agcgcggcga
     2161 ggagccaagc ggccgccagg accgcgcccg cgcgccgcca ccgcctggga ccgtccttca
     2221 taggcctccc tcccttctgt gaaagcggtt cggcggtgcg cggggacatc ctcccgcggc
     2281 ggtccgccat ctgcggcctg tacaggttca gatagatccg aaacaccggc ttgtcacgat
     2341 ttggacggca atcgtataat taaaggaagc ctggtgcgcg catcgggccg aggtggtccc
     2401 agggggaatg ccccatggcg tttgtcgaca agacccttca ttgcctcgat tgcgggaagg
     2461 agttcgtgtt caccgccggt gagcaagagt tctacgctaa gaaggggttt acgaacgagc
     2521 cgttgcgctg caaggagtgc cgggacgccc ggaagcggtc ccgcgaggga gcggcgggga
     2581 tgccgcgtga gatgttcgat gccgtctgtg cgaagtgcgg tgcgaagacc caggttccgt
     2641 ttcgccctcg ggaggatcgg ccggtgtact g
//
LOCUS       DASQGH010000458         8231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38874, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000458 DASQGH010000000
VERSION     DASQGH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38874"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4304)
                     /locus_tag="VEW91_09035"
     CDS             complement(<1..4304)
                     /locus_tag="VEW91_09035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYM91763.1"
                     /translation="MNADTARASPDPASLGPRPDPLAGFHPLVARWFAGRFAAPTAPQ
                     SLGWPRIASGQHVVIAAPTGSGKTLAAFLWSINRLILAAAGGELADRMDVIYVSPLKA
                     LGNDIERNLREPLEEIRRLAVEAGSPLPEIRVTVRSGDTPARDRARMLRRPPHILITT
                     PESLYILLTAQGSRAALATARTVIIDEIHAVAGDKRGAHLALSLERLDALCGRRLQRI
                     GLSATQRPIEEIAHLLVGAEGCAVADIGHRRALELRIEVPDQELGPITTHALWEEVYD
                     RIVRHARRHRATLVFVNTRRQVERVAHALSARLGEGRVAAHHGSLSRPVRLAAEQRLK
                     SGEVPVVVATASLELGIDIGHVDLVCHIGAPRSLATLLQRVGRSGHWLGAVPRGVFFP
                     LTRDDLVQCAAAVRAVRAGDLDRTRLVDKPLDVLAQQMVAAVAAAEMGEEELWDLVRR
                     AWPYRALARSEFEAVLEMLCEGVAGRRGRRGAHIHRDRVHGRLRPRRGARLTAITCGG
                     AIPDTGDYDVVEEVTGALVGRLNEDFAIESQSGDIFLLGNTSWRIRRITSGRVLVENA
                     HGLPPSAPFWLGEAPARTRELSCAVAELRAAVAERLPPASLSGGEPEAAVRWLGEETG
                     LDAAGAQQLVGYIAATRAALGAVPTQQVVIAERFFDEGGGMQLVLHAPFGGRITRAWG
                     LALRKRFCLTFDFELQAAATDDGLVLSLGEQHSFPLESVFGMVRRATLEDDLVQALLA
                     APMFANRWRWNATRALAVRRQEGGRRVPIALQRMRADDLMAAVFPEQVACGDNHAGPV
                     VPPDHPLVNETIGNCLHEAMDTEGLRAVLAAIERGEIRTVAVETPAPSPMSHEILNAN
                     PYAFLDDAPLEERRARAVSLRRSVPELAGTLGRLDPAAVEEVQAQSWPDIRTPHELHD
                     LLLTVGLLPAGDAGAWRAMADALVADGRAAVAEWTLDGREHAAYVAAERLPWLWALRR
                     EARLTPPLSAPAGVEREVSGEEAAQRIIRGWMECLGPATADALGARLGLPRRMVDHAL
                     VSLEASGGLLRGRFTADAPPDTVEWCDRRLLSRIHRLTLGRLRREIEPVAPAAFMRFL
                     LRWQHLHPGTQLHGRAGVLEVITQLQGLELPATAWEAQVLPGRIHAYDPTDLEHLCLA
                     GRIAWGRLHPPHEDPDGSAPAGRRRWTLPRQAPLAFVLRDDLPAFVGPAPDLGVPGDL
                     GGAAAEVFQALQRYGASFLPDIARAVRMLPAQVEAALWELVARGLVSGDGIAGLRFLL
                     RGSRYRRRRGGHHAVEMPLGRWALWRTAAAEPPPSQEKRREVVARQYLRRYGVVFREI
                     VARETQAPPWRTLLQIYRGWEASGEIRGGRFVDGVIGEQYALSQAVEALRAVRRQAPD
                     GETAAVAAADPLNLVGIVLPGPRVPVTSGHVIVYRDGLPVASGPLGVVRSHLQPVA"
     gene            4463..6052
                     /locus_tag="VEW91_09040"
     CDS             4463..6052
                     /locus_tag="VEW91_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYM91764.1"
                     /translation="MSVRTPVPPSPRSPARPLRWAKVIDHTRCIGCHACTTACKSENE
                     VPLSVTRTYVKSVDVGLFPQARRVFQVTRCNQCDDAPCVTACPTTAMYRRPDGIVDFN
                     KAICIGCKACIAACPYDAIFINPDDHAAEKCNFCAHRIDMGLEPACVVVCPTEAILVG
                     DLNDPASRVAQIVHREPVTVRRPEKETHPKLFYKGAHQATLDPIAARRPAGGLYMWSE
                     QGGPDQIPSGHPNGWSNSSAAALLSYDVAHQAPWGWQVSLYTWTKAIAAGAYLVPLLL
                     ILGGWLSWTSVLWFWSAPVLAGIFLAATGALLIADLKHPERCYLIFTRPQWSSWLVKG
                     AFIIAGYGGVLAAHVAAAILGGTRVQAALAIVGLPLAVLTAVYTAYLFAQAKARDLWQ
                     NPLLPPHFFVQAALAGSAALVPLAMRFEPDLVRPLLWTLGGASLLQLLFVWGEATLTH
                     ATAHARLAVHEMVGGRYRAFFRSGIALTAVAMLACGLGGAGVLPALGPWVGLAAVPLA
                     LAGLLAYEHAYVQAGQAVPLA"
     gene            6063..>8231
                     /locus_tag="VEW91_09045"
     CDS             6063..>8231
                     /locus_tag="VEW91_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYM91765.1"
                     /translation="MDLEKLERLVSAARSAAEARGETFYPGPSRIHLAAFPPKERWND
                     WVELDSKAWPARVEKHYMLVPTTCFNCESACGLLAYVDRETLQVRKFEGNPEHPGSRG
                     RNCAKGPATLNQVTDPDRILYPLKRTGRRGGGAGVRVSWDEALDDIATRVRGAITGGR
                     ANEIMYHVGRPGEDGFTERVLAAWGVDGHNSHTNVCSSGGRAGYHFWMGLDRPSPDHA
                     NARVILLVSSHLESGHYFNPHAQRIMEAKQRGAKLIVFDTRLSNTATHADHWVAPYPG
                     SEAAILLAIANDLIQRNLYNREFVRRWWNWQEYLEREHVKLPPTFDNFEGVLKTLYAG
                     YTFEFAAAESGVDAQVLRDVAQIVASAGTRLATHTWRSASAGNLGGWQVARSLFFLNA
                     LLGAVATEGGTSPNAWNKFVPRPIYLPPHPPAWNELTWPREYPLALNEMSFLLPHLLR
                     EGRGRLQVYFTRVYNPVWTNPDGFSWIEALTDADRVGLHVALTPTWNETAFFADYILP
                     MGHGSERHDLHSYEQYDGQWIAFRQPVLRAARARLGERFADTRQVNPGEVWEENEFWI
                     ELSWRIDPDGQLGIRRYFESQASPGQKLTVDEYYGYIFEHSVPGLPERAAAEGLTPLE
                     YMRRYGAFEVARRIGPAYEERVPPQEFDDLQIGWAGRAYTRAPAPPTPNIVPAGTPEP
                     DAHGRRPVGVKIEGEILRGFPTPSGRLEFYSSTLADWGWAR"
BASE COUNT         1199 a         3049 c         2868 g         1115 t
ORIGIN      
        1 taggcgacag gttgcaggtg gctccgcacc accccgaggg gacctgaagc gaccggcagc
       61 ccatcgcggt agacgatcac gtggcccgag gtgaccggta cccgggggcc ggggagcacg
      121 atgccgacca ggttgagggg atcggcggcc gcgaccgctg cggtctcccc gtccggggcc
      181 tggcggcgca cggcccgcag cgcctccacc gcctgggaga gcgcgtactg ctcgccgatc
      241 accccatcca cgaaccggcc cccgcggatc tcccccgagg cctcccaccc gcggtagatc
      301 tggagcagcg tccgccacgg gggagcctgc gtctccctgg cgactatctc gcggaagacg
      361 acgccgtagc ggcggaggta ctggcgggcg accacctccc gccgcttctc ttgggacgga
      421 ggcggctcgg cggccgcggt ccgccacagc gcccaccggc ccagaggcat ctccacggcg
      481 tggtgccctc cgcgacgtcg gcggtaccgg ctcccccgga ggagaaaccg cagcccggcg
      541 atcccgtcgc cggaaacaag cccccgtgcc accaactccc acagcgcggc ttccacctgc
      601 gcgggcagca tgcgaaccgc ccgcgcgata tcggggagga aggatgcccc gtaccgctgc
      661 aacgcctgga agacctcggc ggccgccccg ccgagatccc ccggcacccc gaggtcgggc
      721 gcgggcccga caaacgccgg aagatcgtcg cgcagcacga acgcgagcgg ggcctgccgc
      781 ggcagggtcc accggcggcg gcccgcgggg gccgacccat cagggtcctc atggggggga
      841 tgcaggcgtc cccacgcgat cctgcccgcc aggcacagat gctcgagatc cgtcgggtcg
      901 taggcgtgaa tgcggccggg gagcacctgc gcttcccacg ccgtggcggg aagctccagc
      961 ccctggagct gggtgatgac ctcgagcacc cccgcccggc cgtgcagttg ggtgccgggg
     1021 tggaggtgct gccaccgcag caggaaccgc atgaatgctg ccggagccac cggctcgatc
     1081 tcccgccgca ggcgccccag ggtcaggcgg tggatccggc tcagaagccg gcggtcgcac
     1141 cactccacgg tgtcgggcgg ggcatcggcg gtgaaccggc cgcggaggag gccgcccgat
     1201 gcctccagcg acacgagcgc atggtcgacc attcgccggg gcaggccgag gcgcgcaccc
     1261 agggcgtccg cggtggccgg gccgaggcat tccatccacc cgcggatgat ccgctgtgcg
     1321 gcctcttctc cgctcacctc gcgctcgacg ccggccggcg cagagagcgg aggcgtgaga
     1381 cgcgcctccc gccgtagcgc ccacagccac gggaggcgct cggccgccac atacgcggcg
     1441 tgctcccgtc cgtccaaggt ccactcggcg acggcggccc gcccgtccgc caccagggca
     1501 tccgccatcg ctctccaggc ccctgcgtcg ccggccggca gcagccccac cgtgagcagc
     1561 aggtcgtgca gttcatgggg cgtgcggatg tccggccagg attgggcctg gacctcctcc
     1621 accgcggcgg gatccagccg cccgagggta ccggccagtt ccgggacgga gcgccggagc
     1681 gagacggcgc gcgcccgccg ctcctcgagg ggcgcgtcgt ccaggaacgc gtacggattc
     1741 gcgttcagga tctcgtgcga catcggcgag ggggccggcg tctccacggc aaccgtccgg
     1801 atctcgccgc gctcgatcgc ggccagcacc gcgcgcaggc cctcggtatc catcgcctcg
     1861 tggagacagt tcccgatcgt ctcgttcacc agggggtggt ccggggggac gaccggcccg
     1921 gcgtgattgt cgccgcacgc cacctgctcg gggaataccg ccgccatcag gtcatcggcc
     1981 cgcatgcgct gcagggcgat cggcacccgg cggccgcctt cctgccgccg caccgcgagc
     2041 gcccgcgtgg cgttccaccg ccaccggttg gcgaacatcg gcgccgcgag cagggcctgc
     2101 accaggtcgt cctcgagcgt cgcgcgccgg accatcccaa acacgctctc cagcgggaac
     2161 gagtgctgct ccccgagcga aaggacgagc ccgtcgtccg tcgcagccgc ctggagctcg
     2221 aagtcgaacg tcaggcagaa ccgcttccga agcgccagcc cccacgcccg cgtgatccgc
     2281 cccccgaacg gggcgtgcag cacgagctgc atccccccgc cctcgtcgaa gaaccgctcg
     2341 gcgatcacca cctgctgggt cgggacggca ccgagcgccg cccgggtcgc tgcgatgtac
     2401 cccacgagtt gctgggcgcc ggccgcgtcc aggccggtct cctcacccag ccagcggacc
     2461 gccgcctcgg gctccccccc actgagggag gcaggcggca gccgctcggc caccgccgcg
     2521 cgcaactccg cgacggcgca ggagagctcc cgggtgcgcg ccggcgcctc ccccagccag
     2581 aacggggcac tcggcggcaa tccgtgcgcg ttctccacca ggacacggcc ggaggtgatc
     2641 cgccggatcc gccacgaggt gttgccgagg agaaagatat cgccgctttg gctctcgatg
     2701 gcaaagtcct cgttcaggcg gccgaccagg gccccggtga cttcctcgac gacgtcgtaa
     2761 tcgccggtgt cggggatcgc cccgccacag gtgatcgcgg tcaaccgcgc cccccggcgg
     2821 ggccgcagcc gcccgtgcac ccggtcccgg tggatgtggg cgccgcggcg tccccgccgc
     2881 cccgccacgc cctcgcacag catctccagc accgcctcga actcgctccg cgcgagggcc
     2941 cggtacggcc aggcgcgccg caccagatcc cacagctcct cctcccccat ctcggccgcc
     3001 gccacggcgg cgaccatctg ctgggccagc acatcgagcg gcttgtccac gagccgggtg
     3061 cgatcgaggt ctccggcgcg gacggcccgg acggccgccg cgcactggac caggtcgtcg
     3121 cgggtgaggg ggaagaacac ccctcgcggc accgccccga gccagtggcc cgatcgcccg
     3181 acccgctgga gcaatgtcgc gagcgagcgg ggggccccga tatggcagac caggtcgacg
     3241 tggccgatgt cgatccccag ttccaacgac gccgtggcca ccacgaccgg gacctcgccc
     3301 gacttgagcc gctgctccgc ggcgagccgc accggccggc tgaggcttcc gtggtgtgcg
     3361 gccacccggc cctcgccgag ccgcgcgctc agcgcgtggg cgacgcgctc cacctgccgg
     3421 cgcgtgttca cgaacaccag ggtcgcgcgg tgccggcggg cgtggcggac gatgcggtcg
     3481 tagacctcct cccagagggc gtgcgtggtg atcgggccaa gctcctgatc gggcacctcg
     3541 atgcgtaact ccagggcgcg gcggtggccg atgtccgcga ccgcgcaccc ctcggcgccg
     3601 accaggaggt gcgcgatctc ttcgatcggc ctctgggtgg cgctgagccc gatgcgctgg
     3661 aggcgccgcc cgcagagcgc gtccagccgc tcgagcgaga gcgcgaggtg cgccccccgc
     3721 ttgtcccccg ccacggcgtg gatctcgtcg atgatcacgg tccgggcggt ggcgagcgcg
     3781 gcacggctgc cctgggccgt cagcaggatg tacaacgact cgggggtggt gatcaggatg
     3841 tgcggagggc gccggagcat ccgggcccgg tcgcgggccg gggtgtcgcc cgaccgcacg
     3901 gtgacgcgga tctccggcag cgggctcccc gcctccaccg ccagccggcg gatctcttcg
     3961 agcggctctc tcaagtttct ctcgatgtcg ttgcccagcg ctttcagggg agagacgtag
     4021 atcacatcca tgcgatcggc cagttccccg ccggccgccg ccaggatgag gcggttgatg
     4081 gaccagagga acgcggcgag ggtcttcccg gaaccggtgg gggccgcgat gaccacgtgc
     4141 tgccccgagg cgatgcgcgg ccaccccaga gactgcggcg cggtgggagc ggcgaaccgg
     4201 ccggcgaacc agcgcgcgac cagcgggtgg aaccccgcga ggggatcggg ccggggcccg
     4261 agggacgccg gatctggaga tgcgcgcgcc gtatccgcgt tcacggcggg gatattccat
     4321 cgtgaacgaa cgttttcccc ttgacatccc cggcccccgg tccgtcccgg cgagcgctcc
     4381 actgaccgcc gcgcgccggg gctgaatcca caagagcagg aatcgcctgc gcgaatgcgg
     4441 taggtggaga tttatgggcg ctgtgagcgt gcgcaccccc gttcccccgt ccccccgctc
     4501 cccggcgcgc cccctgcggt gggccaaggt catcgatcac acgcggtgca tcggctgcca
     4561 cgcctgtacg accgcgtgca agtccgagaa cgaggtcccg ctctcggtca cccgcaccta
     4621 tgtgaagtcc gtggacgtcg gactcttccc ccaggcgcgc cgcgtgttcc aggtgacccg
     4681 ctgcaaccag tgcgacgacg cgccctgcgt caccgcctgc cccaccacgg cgatgtaccg
     4741 gcggccggac gggatcgtcg acttcaacaa agccatctgc atcgggtgca aagcgtgcat
     4801 cgcggcctgc ccgtacgatg ccatcttcat caaccccgac gaccatgcgg cggagaagtg
     4861 caacttctgc gcgcatcgca tcgacatggg gctcgagccg gcgtgcgtgg tggtctgccc
     4921 caccgaggcg atcctcgtgg gcgatctcaa cgatccggca tcgcgtgtgg cccagatcgt
     4981 ccaccgcgag cccgtcacgg tgcggcgccc cgagaaggag acgcacccca agctcttcta
     5041 caagggtgcg caccaggcca ccctcgatcc gatcgccgcg cgccgtccgg cgggcggcct
     5101 gtacatgtgg agcgagcagg gcggccccga ccagatcccc tcgggccacc ccaacgggtg
     5161 gagcaactcc tcggcggccg cgctgttgag ctacgatgtg gcgcaccagg ccccctgggg
     5221 atggcaggtc agcctctaca cctggaccaa ggcgatcgcg gcaggagcgt atctggtccc
     5281 gctgctgctg atcctgggcg ggtggttgtc gtggacgagc gtcctgtggt tctggtcggc
     5341 tcccgtgctg gccgggatct tcctcgccgc gaccggagcc ctgctcatcg ccgacctcaa
     5401 gcacccggag cggtgctacc tgatcttcac gagaccccag tggtcgagct ggctggtgaa
     5461 gggcgcgttc atcatcgcgg ggtacggcgg ggtgctggcc gcccacgtgg cggccgcgat
     5521 cctggggggg acccgggtcc aggccgccct ggccatcgtc ggactgccgc tggcggtgct
     5581 gaccgcggtg tataccgcct acctcttcgc ccaggccaag gcccgggacc tctggcagaa
     5641 ccccctcctg cctccgcact ttttcgtgca ggcggcgctc gccggctccg ccgcgcttgt
     5701 gccgctcgcg atgcggttcg agccggatct cgtgaggccg ctgctctgga cgctgggggg
     5761 tgcgagcctg ctccagctcc tgttcgtctg gggcgaggcc acgctgaccc atgcgaccgc
     5821 ccacgcgcgg cttgcggtgc acgagatggt cggcggacgg taccgggcgt tcttccgctc
     5881 tggcatcgcg ctcactgcgg tggcgatgct cgcctgcggg ctcggggggg cgggcgtcct
     5941 cccggcgctc gggccgtggg tggggcttgc cgccgtccca ctggcgctgg ccggcctgct
     6001 ggcctacgag cacgcgtacg tccaggcggg gcaggcggtc ccgctggcct agggaggacg
     6061 gcatggacct cgagaaactc gaaaggctgg tgagcgccgc acgcagcgcg gcggaggccc
     6121 ggggagaaac gttctacccc ggcccgagcc ggatccacct cgcggccttt ccacccaagg
     6181 agcgctggaa cgactgggtg gagctggatt cgaaggcctg gccggcgcgg gttgaaaagc
     6241 attacatgct cgtgcccacg acctgcttca actgcgagtc cgcctgcggg ctcctggcgt
     6301 acgtggaccg ggagaccctc caggtgcgga agttcgaggg caaccccgag cacccgggat
     6361 cccgcggccg caactgcgcc aagggcccgg ccacgctcaa ccaggtcacg gaccccgatc
     6421 ggatcctcta cccgctcaaa cggaccgggc ggcgcggggg aggcgcgggg gtgcgggtca
     6481 gctgggacga ggccctcgac gacatcgcga cccgagtgcg cggggcgatc accggggggc
     6541 gcgccaacga gatcatgtat catgtgggac ggccggggga ggatggcttc accgagcggg
     6601 tgctcgcggc ctggggcgtc gatgggcaca actcccacac caacgtctgc tcgagcggcg
     6661 ggcgggcggg ctaccacttc tggatggggc tggaccgtcc gagcccggac cacgcgaacg
     6721 ccagggtcat cctgctggtc agcagccacc tggagtcggg ccactacttc aacccccacg
     6781 cgcagcggat catggaagcc aagcagcggg gggcgaagct gatcgtgttc gatacgcggc
     6841 tctcgaacac cgccacgcac gccgaccact gggtggcccc ctaccccggg agtgaggcgg
     6901 cgatcctcct ggccatcgcc aacgacctga tccagcgcaa cctgtacaat cgcgagttcg
     6961 tgcgccgttg gtggaactgg caggagtacc tcgaacgcga gcacgtcaag ctccccccca
     7021 ccttcgacaa cttcgagggg gtcctcaaga cgctgtatgc cgggtacacg ttcgagttcg
     7081 ccgccgccga gtccggggtg gacgcccaag tactcaggga cgtcgcccag atcgtcgcgt
     7141 ccgccggcac ccggctcgcc acgcatacgt ggcgcagcgc gtccgccggc aacctcgggg
     7201 gctggcaggt ggcgcggagc ctgttcttcc tcaatgccct gctgggggcc gtcgcgaccg
     7261 aagggggcac gtcccccaac gcctggaaca agttcgtgcc caggccgatc tacctgccgc
     7321 cgcacccccc cgcctggaac gagctcacct ggccccgcga gtatccgttg gcgctcaacg
     7381 agatgtcctt cctcctcccg cacctgctcc gggagggccg gggccgcctc caagtctact
     7441 tcacccgggt ctacaacccc gtgtggacca accccgacgg gttctcctgg atcgaggccc
     7501 tgaccgatgc ggaccgcgtg ggcctccatg tggcgctcac gccgacgtgg aacgagacgg
     7561 cgttcttcgc ggactacatc ctgccgatgg gccacggctc cgaacggcac gacctgcact
     7621 cctacgagca gtacgacggc cagtggatcg cgttccggca gcccgtgctc cgcgcggccc
     7681 gcgcgcggct gggggagcga ttcgccgata cccggcaggt caacccgggg gaggtgtggg
     7741 aggaaaacga gttctggatc gagttatcct ggcggatcga tccggacggg cagctcggga
     7801 tccggaggta cttcgagtcc caggcctcgc ccgggcagaa gctcaccgtc gacgagtact
     7861 acgggtacat cttcgagcac agcgtccccg gcctgcccga acgggcggcg gccgaggggc
     7921 tgacgcccct cgagtacatg cgccggtacg gggcgtttga ggtcgccagg cggatcggcc
     7981 cggcctacga ggagcgcgtc cccccgcagg aattcgacga tctccaaatc ggttgggccg
     8041 gccgggccta cacgagggcc ccggcccccc ccacccccaa catcgtgccc gccggaaccc
     8101 ccgagccgga tgcccacggg cgccgccccg tcggggtaaa gatcgagggg gagatcctgc
     8161 gcggctttcc cacgccgtcc gggcggctcg agttctactc atccaccctg gccgactggg
     8221 gatgggcgcg c
//
LOCUS       DASQGH010000459         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50090, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000459 DASQGH010000000
VERSION     DASQGH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50090"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VEW91_09050"
     CDS             complement(<1..385)
                     /locus_tag="VEW91_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91766.1"
                     /translation="MSTPLLALQNVDTYYGDSHILQGVTLAVEPATITCVLGRNGAGK
                     TTAVRTVMGFTPPRGGRVAFKGRDITGMPPHRVARAGIALVPQGRGIFPDLTVRQQLT
                     LAIRPGGWPLGRVYERFSILAERADQ"
     gene            complement(382..1140)
                     /locus_tag="VEW91_09055"
     CDS             complement(382..1140)
                     /locus_tag="VEW91_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91767.1"
                     /translation="MGAVLITEHLTRRFGGVLALNDVSLEVEAGERHAVIGPNGAGKT
                     TLFNLITGELLPSSGRVLLDDRPVTGLAPHKIAAQGVARTFQRNNLLFNLTALENVRL
                     AVQARTTATRRVLTPTAHLRDLHEAALALLARVGLEARAEVAARALSYGEQRQLELAI
                     ALAGRPRVVLLDEPTSGMSPAETATMTALLARLGRGQTLVIIEHDMDVVFAIADRITV
                     LHLGEVLARGTPDTVRGHPRVQEVYLGAPPPAHP"
     gene            complement(1110..2057)
                     /locus_tag="VEW91_09060"
     CDS             complement(1110..2057)
                     /locus_tag="VEW91_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM91768.1"
                     /translation="MRAAAARTAWIAAGLGMALVPLAHHRYLTTTLTEILIFALFAMS
                     LGLLVGYAGLVSLGHAAFFGTAAYAVGLVSTRVSPALLLTLPAGTAAGTLAALVIGVF
                     ALRATGVYFLMLTLAFAQMAYAVAHQWVWLTGGTNGLSGIPRPAIPGVDVGGAVPFYY
                     LVLLVVGASALLLSRIVASPFGAALVGIRENEPRMRAMGFDTFRLKLAAFVLAGAAAA
                     LSGTLYAYYNGFVSPDDLYWTRSGEVLVMVLLGGAGTLAGPAVGAAAVLVLQNLASSF
                     TERWTMILGAAFILVVIAAPRGLAGLVAGRWARSSSPST"
     gene            complement(2054..>2634)
                     /locus_tag="VEW91_09065"
     CDS             complement(2054..>2634)
                     /locus_tag="VEW91_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM91769.1"
                     /translation="LLTFGFAFVLSDLARWWWGADVQSLAPPPGLDRSIPLLGTLFPS
                     YRLFVIAFGAVLAGGLWIGLSRTRLGAMVRAGVANREMTQALGIDIGLVFTGVFAFGA
                     ALAGLAGVIAAPIQGVFPGVDFETLIVTLIVVVVGGLGTVTGSFWGSLLIGEVDTFGK
                     TLVPQIALVAIYLVMAAVLLVRPAGLFGGRTR"
BASE COUNT          442 a          947 c          943 g          302 t
ORIGIN      
        1 gctgatcggc gcgctcggcc aggatcgaga atcgctcgta gacacggcca agcggccacc
       61 cgcccggccg gatggccagg gtgagctgct ggcggaccgt gagatcggga aagatccccc
      121 ggccctgcgg gaccagcgcg atgccggcgc gggccacgcg gtgcgggggc atcccggtga
      181 tatcgcggcc cttgaaggcc acccggcccc cgcggggcgg ggtgaacccc atgaccgttc
      241 ggaccgccgt cgtctttccg gccccgttcc ggccgagcac gcacgtgatg gtcgccggct
      301 cgaccgccag ggtcacgccc tggaggatgt gcgagtcccc gtagtaggtg tcgacgttct
      361 ggagcgccag gagcggggtg ctcatggatg cgccggcggc ggggcgccga ggtagacttc
      421 ctggacccgg ggatgtccgc ggaccgtgtc gggggtcccg cgcgccagca cctccccgag
      481 gtgcagcacg gtgatccggt cggcgatcgc gaacaccaca tccatgtcgt gctcgatgat
      541 gacgagcgtc tgcccgcgcc cgaggcgggc gaggagcgcg gtcatagtgg ccgtctcggc
      601 cggagacatc cccgacgtcg gctcgtcgag gagcacgacg cgcggccgcc cggcgagcgc
      661 gatcgcgagc tcgagctgcc gctgctcccc gtaggacagc gcccgcgccg caacctccgc
      721 cctggcctcg agccccacgc gggccaggag ggcgagcgcc gcttcgtgga ggtcgcgaag
      781 atgcgcggtg ggcgtcagca cccgccgggt cgccgtcgtg cgcgcctgca ccgcgaggcg
      841 gacgttctcg agcgcggtga ggttgaacag gaggttgttg cgctggaagg tgcgggccac
      901 gccctgcgcg gcgatcttgt gcggggccag ccccgtcacc ggacggtcgt ccaggaggac
      961 gcggcccgac gaggggagga gctcgccggt gatgagattg aacagggtgg tcttgcccgc
     1021 tccgttgggg ccgatgacgg cgtgccgctc ccccgcctcc acctcgaggc tgacgtcgtt
     1081 cagggcgagc acgcccccga accggcgggt caggtgctcg gtgatgagga ccgcgcccat
     1141 cgtcctgcca caagccccgc cagcccccgg ggcgcggcga tgacgaccag gatgaacgcc
     1201 gcgcccagga tcatcgtcca ccgctcggtg aacgaactgg ccaggttttg gagcaccagc
     1261 acggcagcgg ccccgaccgc cggccctgcc agcgtgcctg cgccgccgag cagcaccatg
     1321 accaggacct ctccggaccg cgtccagtag aggtcgtccg gggagacgaa cccgttgtag
     1381 tacgcgtaca gcgtgccgct cagcgcggcc gccgctccgg ccagcacgaa cgccgccagc
     1441 ttgaggcgga aggtgtcgaa ccccattgcc cgcatccggg gttcgttttc ccggatcccc
     1501 acgagggccg cgccgaacgg ggaggcgacg atcctcgaga gcaggagcgc ggaggcgccg
     1561 accacgagga gcaccaggta gtagaacggc accgcaccgc cgacgtcaac cccggggatc
     1621 gccggccggg ggatccccga gagcccgttc gtgccgcccg tgagccacac ccactggtgc
     1681 gccaccgcgt acgccatctg ggcgaacgcg agcgtcagca tgaggaagta caccccggtg
     1741 gcgcgcagcg cgaacacccc gatcacgagc gcggccaggg tcccggccgc ggtgcccgcg
     1801 ggaagggtga ggagcagcgc cggactcacg cgcgtgctca cgaggcccac cgcgtacgca
     1861 gcggtcccga agaacgccgc gtgcccgagg gaaaccaggc cggcgtaccc cacgagcagg
     1921 cccaggctca tcgcgaagag ggcgaagatc aggatctccg tgagggtcgt ggtgagatac
     1981 cggtggtgtg caaggggcac gagcgccatc cccaggccgg cggcgatcca ggcggtacgc
     2041 gcggcggccg ccctcaccgg gtccgacccc caaagagccc ggcgggccga acgagcagga
     2101 ccgcggccat caccagatag atggcgacca gcgcgatctg gggcaccagg gtcttcccaa
     2161 acgtgtctac ctcgccgatc agcaggcttc cccagaacga tccggtgacc gtgccgagcc
     2221 cgccgaccac caccacgatg agcgtgacga tcagggtttc gaagtccacc ccggggaaca
     2281 ccccctgaat gggggccgcg atcacgccgg cgagccccgc cagcgccgcg ccgaacgcga
     2341 acacccccgt gaacacgagc ccgatatcga tgccgagcgc ctgggtcatc tcccggttgg
     2401 cgacccccgc ccggaccatc gcgccgaggc gggtgcggct caatccgatc cagagccctc
     2461 cggccaacac cgcgccgaac gcgatcacaa agaggcggta ggacgggaac agcgtgccga
     2521 gcagagggat ggaccggtcc agcccgggag gaggggcaag cgactgcacg tcggcccccc
     2581 accaccaccg cgccaggtcc gacagcacga acgcgaaccc gaaggtcagc agga
//
LOCUS       DASQGH010000460         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_1942, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000460 DASQGH010000000
VERSION     DASQGH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VEW91_09070"
     CDS             <1..762
                     /locus_tag="VEW91_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYM91770.1"
                     /translation="AGSGTIFFSMCNLRCVFCQNYEISQLRRGREVLPQELARMMLSL
                     QDQGCHNINLVTPEHVVPQVLEALPPAVEMGLRLPLVYNTSSYDSAHSLRALDGIVDV
                     YMPDFKFWDREMSRFYLKAPDYPEVARRAIKEMHRQVGPLKLDEFGLARRGVLLRHLV
                     MPGGIAGTREILRWVAQEVSTHTYVNVMDQYNPAWKVGVEHRYDAINRRITPEEFREA
                     LEAAREAGLYRFDVRVPRDQRIRWMPHPAAALHSV"
     gene            complement(815..>2281)
                     /locus_tag="VEW91_09075"
     CDS             complement(815..>2281)
                     /locus_tag="VEW91_09075"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HYM91771.1"
                     /translation="MGPAEVGALVAAGVREVCVRRRPVVWLIPTGNEIVDPAGELRPG
                     QIPEFNTAMLAPEVNSWGGVGLRHSIVPDDPDQLVDVLKRAAPCDLLVTIAGASSGAR
                     DYTAEAFSRLGEVVVHGVAIRPGKPVVLGIVNGRPAIGLPGYPVSAALTFDLFARPAV
                     YALLGVAPPERERLRATLVRKIHSTAGVDEFVRVTVGRVNNRIVAAPLARGAGLITSL
                     VRADGWLVIPRTSEGIEAGAEVEVELRHSSAAVESTIMVVGSHDVALDVLADFLRRAN
                     PKSTLASAHVGSLGGLLALRRGEAHMAGVHLLDEETGDYNVSYVRRYLPGRPTALVTL
                     AQRDQGLLVAPGNPKGIRSVADLARADVQFVNRQRGAGTRVLLDYELRRAGLSPQQIQ
                     GYDREVYTHMAVAVAVATGAADCGLGILAAARAMGLDFVPVARERFDLAIPEDQMESP
                     LVKQVLAVLDEPEFRRAVTALGGYDLSLTGTVVQVNPA"
BASE COUNT          376 a          813 c          737 g          355 t
ORIGIN      
        1 gcaggcagcg gcaccatctt cttctccatg tgcaacctgc gctgcgtctt ctgccagaac
       61 tacgagatca gccagttgcg ccgtgggcgc gaagttctcc ctcaggagct cgcccgcatg
      121 atgctctccc tgcaggacca gggatgccac aacatcaacc tcgttacgcc cgagcatgtc
      181 gtcccgcagg tcctggaggc attgccgccc gccgtcgaga tggggctgcg cctccccctt
      241 gtctacaaca cgagcagcta cgactcggcg cactccttgc gggccctcga cgggatcgtg
      301 gacgtctaca tgcccgactt caagttctgg gacagggaga tgagccggtt ctacctgaag
      361 gcacccgact atcccgaggt cgcccggcgg gccatcaagg agatgcaccg gcaggtcggc
      421 ccgctgaagc tcgatgagtt tggcctggca aggcggggtg tgctgttgcg gcacctggtg
      481 atgcccggcg ggatcgcggg aacccgcgag atcctccgct gggtcgcaca ggaggtctcg
      541 acccacacct acgtcaacgt catggatcaa tacaatccgg cgtggaaggt cggcgtcgag
      601 cacaggtatg acgccatcaa ccgccggatc acccccgagg agtttcgcga ggccctcgag
      661 gccgcccggg aggcgggtct gtaccgcttt gatgtgcggg tgcctcggga ccagaggata
      721 cgttggatgc cgcatcctgc cgcagcactc cacagcgtct gaggtcgcca ccgcctctcc
      781 tggggcggtg atagatcttc gtgcaacacc gaggctaagc gggattgact tgtaccactg
      841 tccccgtcag cgagaggtcg tatccgccta gcgcggtcac cgcgcgccgg aactccggct
      901 cgtcgaggac ggcgagcacc tgcttcacga gtggcgactc catctggtcc tccgggatgg
      961 ccaagtcgaa ccgctcgcgg gccacgggca cgaagtccag acccatggcc cgcgcggcgg
     1021 ccaggatccc cagcccgcaa tccgccgccc ccgtcgcgac ggctaccgcg acggccatgt
     1081 gcgtgtagac ctcccgatcg tatccctgga tttgctgcgg ggacagaccg gcacgccgca
     1141 gttcatagtc cagcaacacc cgggtgccgg cgccccgctg gcggttgacg aactgcacgt
     1201 cggctcgggc aaggtcggcg acgctcctga tgcccttcgg gttgccgggc gcgaccagca
     1261 gcccctggtc tcgctgggcc agcgtcacga gcgccgtcgg ccggccgggg aggtaccgcc
     1321 ggacgtatga gacgttgtag tcgccggtct cctcgtcgag cagatgcaca ccggccatgt
     1381 gggcctcgcc gcggcggagg gccagcaacc ctcccaggct gcccacgtgc gccgaggcca
     1441 gcgtgctctt cgggttcgca cgccgcagga agtccgccag cacgtcaagc gccacgtcgt
     1501 ggctgccgac gaccatgatg gtggactcaa ctgcggccga cgaatgacgt agttcaacct
     1561 ccacctccgc cccggcctcg atcccctcgc tcgtccgcgg gatgacgagc cagccgtcgg
     1621 cccgcaccag cgatgtaatc aaccccgctc cccgcgccag gggcgcggcc acgatccggt
     1681 tgttgacccg gccgaccgtg acccttacga actcgtccac tccggcggtt gaatggatct
     1741 tccggacgag cgttgcccgc aggcgctccc gctccggcgg cgccaccccg agcagcgcat
     1801 agaccgccgg gcgggcaaac aggtcaaagg tgagggcggc tgagacggga tagcccggta
     1861 acccgatggc cggtcggccg ttgacgatcc cgagcacaac cggtttgccg ggccgaatgg
     1921 cgacgccgtg tacgacgacc tcgccgagcc ggctgaaggc ctcggcggtg taatcgcggg
     1981 ctccggacga cgcccccgcg atcgtgacca ggagatcaca cggcgccgct cgcttcagga
     2041 cgtccaccag ttggtcgggg tcatcgggca cgatagagtg gcgcagcccc acgcctcccc
     2101 aactattcac ctcgggtgcc agcatcgccg tgttgaattc cgggatctgc ccggggcgca
     2161 gctccccggc ggggtcgacg atctcatttc cggtcggaat gagccagacc accggccggc
     2221 ggcgaacgca tacctcccgc acaccggcgg ccacgagcgc gcccacctcg gccgggccca
     2281 t
//
LOCUS       DASQGH010000461         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38896, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000461 DASQGH010000000
VERSION     DASQGH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38896"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..403)
                     /gene="yajC"
                     /locus_tag="VEW91_09080"
     CDS             complement(110..403)
                     /gene="yajC"
                     /locus_tag="VEW91_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYM91772.1"
                     /translation="MNAQQVAAQLVQLLPLLAVFVFFYFVFLRPQQLQQRRYREMLGR
                     LKKGDRVLTRGGLYGVVLEVKDNDLMLELAQNVRVRADRTAVQSLVKRGTAGG"
     gene            complement(406..1617)
                     /gene="tgt"
                     /locus_tag="VEW91_09085"
     CDS             complement(406..1617)
                     /gene="tgt"
                     /locus_tag="VEW91_09085"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYM91773.1"
                     /translation="MVLRTCAILRAVALRFDLIHTDRETGARAGVLHTPHGTVRTPVF
                     MPVGTHGTVRALTPEEVEAAGAQIVLANTFHLALRPGAGVIARAGGLHAFMHWERPLL
                     TDSGGYQVFSLPHLRKVADEGVWFRSPIDGQDVAFTPEQIIEIEEHLGADIIMPLDVC
                     LGYPADRAEARDAQRRTVLWARRARDAHRRTDQALFGIVQGGFEIDLRRRAADELVAL
                     DFPGYAIGGLSVGETKALTYDLVAAVAERLPASRPRYLMGVGSPPSVIEAVRRGVDMF
                     DCVLPTRVGRTGTALTEGGPVNLRRAQYAEDLSPVESGCPCPACRHYSRAYLRHLFKA
                     GEMLGPRLVSLHNLAFMARLEAAMREAICEDRLGAWSREVLARYDAGRGPKGNPGRVE
                     EPSATLSRPEG"
     gene            1773..2249
                     /locus_tag="VEW91_09090"
     CDS             1773..2249
                     /locus_tag="VEW91_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91774.1"
                     /translation="MSREERIAAARAKAEAMKAQRAAVAPAQAAPGPSAPPAAGRPAA
                     AAGVPGRATAANPGGAPPTAPQHPKVEAFGTITQSVEFRADPTEAQNLERLLGGLGLY
                     RNPLRGAWQLDYRFFAEARRRLQAAGYQVEGRDYLGRPLEQWAPHTRGWTRLGSPD"
     gene            complement(2274..>2526)
                     /locus_tag="VEW91_09095"
     CDS             complement(2274..>2526)
                     /locus_tag="VEW91_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HYM91775.1"
                     /translation="LLESAAQEDGTIAPFSGDTSLFITPGYRFRAVDVLLTNFHLPRS
                     TLLMLASAFAGRERLLAAYAEAIRQRYRFYSFGDAMLIV"
BASE COUNT          392 a          915 c          889 g          330 t
ORIGIN      
        1 cttcgcgatg tacgcttcaa cctcccgatc ccgcttgacg tcctcgagga tcagcaggcg
       61 ccacggggcg cgcggggtcg ggggcgcggt ggcctggcgt tcctcggcgc tagccaccgg
      121 cggtcccccg cttgacgagc gactgcaccg cggtccgatc cgcccggacc cgaacgttct
      181 gggcgagttc gagcatcaga tcgttgtcct tgacctccag gaccacgcca tagaggcccc
      241 cgcgggtcag cacccggtcg cccttcttga gccgtcccag catctcccgg taccgcctct
      301 gctggagctg ctgcggccgg aggaagacga agtagaagaa cacaaagacc gcgaggagag
      361 gcaatagctg cacgagctgc gccgctacct gctgggcgtt caccctcacc cctccggccg
      421 ggacagagtg gcggagggtt cttcgacacg tccgggattc cctttcgggc cgcggcccgc
      481 atcgtagcgc gcgagcacct cgcggctcca cgctccgagc cggtcctcgc agatcgcctc
      541 gcgcatggcc gcctccagcc gcgccatgaa cgccaggttg tggaggctca ccagccgcgg
      601 gcccagcatc tcgccagcct tgaacagatg gcggaggtag gcccgggagt agtgccggca
      661 ggccgggcac gggcaccccg actcgacggg ggagagatcc tcggcgtact gcgcccgccg
      721 gaggttgacc gggccgccct cggtgagcgc cgtccccgtc cggccgaccc gggtcggcag
      781 cacgcagtcg aacatgtcca cgccccggcg caccgcctcg atcacactcg ggggggaccc
      841 gacccccatg agataccggg ggcgggaagc gggcagccgc tccgcgacgg cggcgacgag
      901 atcgtacgtg agcgccttgg tctccccgac ggagagcccg ccgatcgcgt agccaggaaa
      961 gtcgagcgcg acgagctcgt cggcggcccg ccgccgcagg tcgatctcga acccgccctg
     1021 gacgatgccg aacagcgcct ggtccgttct ccggtgggcg tccctggccc ggcgggccca
     1081 caagacggtg cggcgctggg cgtccctggc ctcggcgcgg tcggcgggat acccgagaca
     1141 cacgtcgagg ggcatgatga tgtcggcccc gaggtgttct tcgatctcga tgatctgttc
     1201 aggggtgaac gcgacgtcct ggccgtcgat gggcgagcgg aaccacaccc cttcatcggc
     1261 cacctttcga agatggggaa ggctgaacac ctggtagccc ccgctgtcgg tgagcagagg
     1321 ccgctcccag tgcatgaagg cgtgcagccc gcccgcccgg gcgatcaccc cggcgccggg
     1381 gcgcagcgcg aggtggaagg tgttggccag cacgatctgg gcgcccgccg cctcgacctc
     1441 ttcgggggtc agcgcccgca ccgtcccgtg ggtgcccacc ggcatgaaca ccggcgtcct
     1501 gaccgtgccg tggggggtgt ggagcactcc ggcccgtgcg ccggtctcgc gatcggtgtg
     1561 gatcagatca aaccgcagag ccacggctcg gagtatagca caggtgcgga gcaccatgac
     1621 cgcgtcgaag gcccgccttg aggtcccggg cgccgccatg tagaatgcag cttgaacgcc
     1681 gaccgcaccc ggagggaacg atgagcgaag aagcgcctcc cgcgcccccg caaccgccag
     1741 cccccggcga gcccgctccg gcgggatccg agatgagccg ggaggaacgg atcgcggcgg
     1801 cccgggccaa agccgaggcg atgaaagcac aacgggccgc ggtcgctccg gcgcaagcgg
     1861 cccccgggcc atcggcgccc ccggcggccg gccgaccggc ggccgcggcg ggggtcccgg
     1921 ggcgggccac cgcggcgaac cccgggggcg cccctccgac cgccccccaa caccccaagg
     1981 tggaagcgtt cggcaccatc acccagagcg tcgaattccg ggcggatccc acggaggcgc
     2041 agaacctcga gcggctcctc ggcgggctcg ggctctaccg gaacccgctc cggggcgctt
     2101 ggcaactcga ctaccggttt ttcgctgaag cccgtcgcag gctccaggcc gcggggtacc
     2161 aggtggaggg ccgcgattac ctgggccgtc ccctcgagca gtgggcgcca cacacccgcg
     2221 gctggacgcg cctcggatcc cccgactgac gccctgcccc ccgcatgggg ggttcacacg
     2281 atcagcatcg cgtctccgaa gctgtagaag cggtagcgct ggcggatcgc ctcggcgtac
     2341 gccgccagca gccgctcgcg tcccgcgaac gcgctggcca gcatcaggag cgtcgaccgc
     2401 ggcaggtgga agttggtgag gagcacgtcg acggcgcgga accggtagcc cggggtgatg
     2461 aacagcgagg tgtcgccgga gaatggcgcg atggttccat cttcctgcgc cgcactttcg
     2521 agcagc
//
LOCUS       DASQGH010000462         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4921, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000462 DASQGH010000000
VERSION     DASQGH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4921"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VEW91_09100"
     CDS             <1..417
                     /locus_tag="VEW91_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM91776.1"
                     /translation="LILIYTAFSLPFAVWMLKGFLAEVPQEVEEAALVDGASRWRAFF
                     RILLPMLSPGLLAAAVITFALSWSEFLYALILTATPRSQTFSIAVWSLVTEFEIIWNQ
                     MAAVGVISALVPVALLLVARRYVIPALTFGAVREKA"
     gene            485..1468
                     /locus_tag="VEW91_09105"
     CDS             485..1468
                     /locus_tag="VEW91_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYM91777.1"
                     /translation="MPVKVGILGAGYIGGVHARTLRRDDRVSLVGIADIRREAAERLA
                     AEVGTRPLPDLEAVLHEGAQAVYVTTPNAHHVEPVVAALGAGVHVFSEKPMATTLEGA
                     HRVAAAAARSAAIYQLGFNRRWAPAYACVHDLIARGDLQPAMAHVKMNRGELQNPPWV
                     STTALTGGFLYESTIHLLDMVRHLLGEVAAVTCHARTTAYQEPDGFAAILRCASGALV
                     TFHSCAHATWVFPFERIEVFGPHQAVVTEEMERVTVSPGLGREITTRNYFQLPMDEKW
                     GYRAEDAKFVDAVLEGRRPGVTAEDGLRATELVEACYRSARAGAEVRLPLA"
     gene            1491..2315
                     /locus_tag="VEW91_09110"
     CDS             1491..2315
                     /locus_tag="VEW91_09110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYM91778.1"
                     /translation="MTDIDGTLVGEDKVVRPGVVGAIAAARARGVRVCLATGRMWAAA
                     RKYVEAVDADPPAILYNGGLLYDFTSGRAIWSRPLALAEARRLLPVLLGFRETSPLMF
                     VQGTVFAERRTPFTDLYARRDSLIIEYAPDLAALLTEPPMKFLVVGDPPDLGRLSQAL
                     AALPPPPVNQVFSQPDYLEILPPGIDKGVALRELARGVGVPLERVVAIGDAMNDLALI
                     RTAGLGVAVEGSPPAVLAAAREICPRPEQEGVRVVIERLFLRGTAAGNAIDEAKEA"
     gene            2318..>2960
                     /gene="iolN"
                     /locus_tag="VEW91_09115"
     CDS             2318..>2960
                     /gene="iolN"
                     /locus_tag="VEW91_09115"
                     /EC_number="3.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-scyllo-inosose hydrolase"
                     /protein_id="HYM91779.1"
                     /translation="MAKWTIPPRGGHMDKRTGIYLQNMTGHEVEERLKANDILLVPLG
                     ATEAHGAHAPLGEDVFLVCRMAEEVAARTGCTVAQPVWYGSHPYHHLGMPGTVVIPEE
                     AFTGYVRAMIAGFWNMGFRKQILLNGHGQEYVIPTAMHQFAKRYRVPSLLLLVNWYHA
                     IRGHFKLTSEGGPYETPFIHADEVETSWSLALFPELLDMKYAPDNKIVGFLPGD"
BASE COUNT          423 a          995 c         1084 g          458 t
ORIGIN      
        1 ctcatcctga tctacacggc gttttcgctg ccgttcgccg tctggatgct caaaggcttt
       61 ctcgccgagg tgccccagga ggtcgaggag gcggcgctcg tcgatggggc cagccggtgg
      121 cgcgccttct ttcggatcct cctcccgatg ctgtcccccg ggctgctcgc cgcggcggtg
      181 atcaccttcg cgttgagttg gagcgagttt ctgtacgcgc tgatcctcac cgccacaccc
      241 cgctcgcaga cattctcgat tgcggtgtgg agcctggtga ccgagttcga gatcatctgg
      301 aaccagatgg ccgccgtggg cgtgatctcc gccctggtgc cggtggccct gctcctggtg
      361 gcccggcgat acgtcatccc ggcgctcacg tttggcgcgg tgcgggagaa agcgtgacgt
      421 cgtatccgag cggaaacccg ccgcggggcg ggcgcagggc agcgcgggag ggagggtggg
      481 gaggatgccg gtgaaggtcg ggattctcgg ggcggggtac atcggcggcg tgcacgcccg
      541 gacgctgcgc cgcgatgacc gggtctcgtt ggtggggatc gcggacatcc gccgggaggc
      601 cgccgaacgc cttgccgccg aggtcggcac ccgtccgctg ccggacctgg aggcggtgct
      661 ccacgaaggc gcgcaggcgg tgtacgtgac gacgccgaac gcccaccatg tcgagccggt
      721 ggtggccgcg ctcggggcgg gcgtgcacgt cttcagcgag aagccgatgg ccacgacgct
      781 cgagggcgcg caccgggtcg ccgccgcggc cgcccggagc gcggcgatct accagttggg
      841 gttcaaccgg cggtgggcgc ccgcctatgc ctgtgtccac gacctgatcg cccgagggga
      901 tctccagccg gcgatggcgc acgtgaagat gaaccggggg gagttgcaga acccgccgtg
      961 ggtgagcacc acggcgctca ccggggggtt cttgtatgag tccacgatcc acctcctcga
     1021 catggtccga cacctgctcg gggaggtcgc cgcggtgacc tgccacgccc ggaccaccgc
     1081 ctaccaggag ccggacgggt tcgcggcgat cctccgatgc gccagcgggg ccctcgtgac
     1141 gttccactcc tgcgcccacg ccacgtgggt gttcccgttc gagcggatcg aggtgtttgg
     1201 gccccaccag gcggtggtga ccgaggaaat ggagcgggtc accgtgagcc cggggctggg
     1261 gcgcgagatc accacccgca actatttcca actgccgatg gacgagaagt gggggtaccg
     1321 ggccgaggac gcgaagttcg tcgacgccgt cctggagggg cggcggccgg gggtgaccgc
     1381 cgaggacggc ctgcgggcga ccgagctcgt ggaggcctgc taccgaagcg cccgggcggg
     1441 ggccgaggtg cgcctccccc ttgcctgagc cggcacctta cgagctcctg gtgaccgaca
     1501 tcgacggcac cctcgtcggg gaggacaagg tcgtccgtcc aggggtcgtg ggggcgatcg
     1561 cggcggcccg cgcgcggggc gtccgcgtgt gtctggccac cgggcgcatg tgggcggcgg
     1621 cgcgaaagta cgtcgaggcg gtggacgccg acccgccggc gatcctctac aacggggggc
     1681 tgctctacga ttttacgtcc gggcgggcga tctggtcgcg ccccctggcg ctggcggaag
     1741 cgcgccgcct cctcccggtc ctcctcgggt tccgggagac ctccccgctg atgttcgtcc
     1801 agggcacggt gttcgccgag cggcgcaccc cgttcaccga tctctacgcg cggcgggact
     1861 cgctcatcat cgagtacgcg cccgatctcg cggctctctt gaccgagccg ccgatgaagt
     1921 tcctggtggt gggggatccc ccggacctgg ggcgcctcag ccaggcgctc gccgcactcc
     1981 cccccccgcc ggtcaaccag gtgttctcgc agccggacta cctcgagatc ctccccccgg
     2041 gcatcgacaa aggggtcgcg ctgcgggagc tcgcccgggg agtcggggtc ccgctcgagc
     2101 gcgtcgtcgc catcggggat gccatgaacg acctcgccct gatccggacg gcggggctcg
     2161 gggtggcggt ggaggggagc cccccggcgg tcctggcggc ggcccgggag atctgccccc
     2221 gccccgagca ggaaggcgtg cgcgtcgtca ttgaacggtt gtttcttcgt gggacggcgg
     2281 ctggaaacgc aatcgacgag gcgaaggagg cgtgagcgtg gcaaagtgga cgatcccgcc
     2341 ccgcggcggg cacatggaca agcggacagg catctacctc cagaacatga cgggccacga
     2401 ggtggaggaa cggctcaaag cgaacgacat cctgctggtc ccgctgggag cgaccgaggc
     2461 gcacggggcg cacgcaccgc tcggcgagga tgtcttcctc gtctgccgga tggcggagga
     2521 ggtcgccgcc cggaccgggt gcaccgtggc ccagccggtg tggtacggat cccaccccta
     2581 ccaccacctc gggatgcccg gcaccgtcgt catcccggag gaggcgttta ccgggtacgt
     2641 ccgggcgatg atcgcggggt tttggaacat ggggttccgc aagcagatcc tcctcaacgg
     2701 gcacggccag gagtacgtga tccccaccgc gatgcaccag ttcgcgaagc gctaccgggt
     2761 ccccagcctg ctgctgctgg tgaactggta ccatgcgatc cggggtcact tcaagctcac
     2821 cagcgagggc gggccgtacg agaccccgtt catccacgcg gacgaggtgg agacctcctg
     2881 gtcgctggcg ctgttccccg aactcctgga catgaagtac gccccggata acaagatcgt
     2941 ggggttcctc ccgggcgacc
//
LOCUS       DASQGH010000463         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_7793, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000463 DASQGH010000000
VERSION     DASQGH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7793"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..461
                     /locus_tag="VEW91_09120"
     CDS             42..461
                     /locus_tag="VEW91_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HYM91780.1"
                     /translation="MAEAPRPPSLSVGRRATFSKTIGGPDIRTFAALTGDRNPLHLDE
                     AFAGRSRFGRPIAHGMLVAGVISAALGTVLPGPGAIYLSQTLKFLRPVYPGDTITATV
                     EVIAYRGDKGIVTLRTTCANQAGDPVVDGEAVLLVGR"
     gene            448..1116
                     /locus_tag="VEW91_09125"
     CDS             448..1116
                     /locus_tag="VEW91_09125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYM91781.1"
                     /translation="MSAVEQIRSIYARWAPGYDLGQWVPERLLLARLRRQLVARARGR
                     ILEVGIGTGANLPYYAASCPVMGVDLSRPMLERALARAYRLGRHLTVEVMDAETLTFP
                     DRTFDAVVSTLTLCTTPDPVRLLREMTRVCRPEGRVLLLEHGMGTTPVINWALRRLAP
                     GNLARCACHLTRDVTSLPVEAGLRVLWQERHLWGVFTLTEAAPRPSPILQARPDATGG
                     LYRG"
     gene            1147..1223
                     /locus_tag="VEW91_09130"
     tRNA            1147..1223
                     /locus_tag="VEW91_09130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1181..1183,aa:Thr,seq:tgt)
     gene            1356..>1555
                     /locus_tag="VEW91_09135"
     CDS             1356..>1555
                     /locus_tag="VEW91_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91782.1"
                     /translation="MRPVRETIQSTTLSDAVGAFLLAKRVANCSSRTIGNHTDVLARF
                     RRESQLTQIHEATVVTIQKYLTV"
BASE COUNT          230 a          538 c          533 g          254 t
ORIGIN      
        1 agaggccccg tggcgctccg gcaggagggc gtgatctaac gatggcggag gctccgcgcc
       61 ccccgagcct ctccgtgggg cggcgcgcca cattttcgaa gacgattggc gggccggaca
      121 tccggacgtt cgcggccctc acgggcgacc gcaatcccct ccacctcgac gaagcgtttg
      181 ccggccggag ccgctttggc cggccgatcg cccacgggat gctcgtcgcc ggggtgatct
      241 ccgcagccct cggcacggtc ctcccgggcc cgggggcgat ctacctcagc cagacgctca
      301 agtttctgcg cccggtctac cccggggaca caatcaccgc gacggtggag gtcatcgcct
      361 accgggggga caaggggatc gtcacgctgc gaacgacctg cgccaatcag gccggcgacc
      421 cggtggtgga tggcgaggcg gtgctgcttg tcggccgttg agcagatccg ttcaatctac
      481 gcccggtggg cgccgggcta cgacctgggc caatgggtcc ccgagcgcct gctgctggcg
      541 cgcctgcgtc gccagctcgt cgctagggcc cgtggtcgga tcctggaggt ggggattggg
      601 acaggcgcca accttccata ctacgccgcg tcgtgcccgg ttatgggggt ggacctgagc
      661 cgcccgatgc tggagcgggc gctggcgcgg gcctaccggc tcggccgtca cctgaccgtg
      721 gaggtcatgg acgcggagac gctgacattc ccggatcgga ccttcgacgc cgtggtgtcg
      781 accctcacgc tctgcacgac ccccgacccg gtccgtctgc tccgggagat gacccgggtc
      841 tgccgtcccg agggccgcgt gctcctcctc gagcacggga tgggcacgac cccggtcatc
      901 aactgggccc tccgccgact cgctcctggg aacctcgccc gctgtgcatg ccacctcacc
      961 cgcgacgtca cgtcgctccc tgtggaagcg ggcctccgcg tgctctggca ggagcggcac
     1021 ctgtgggggg tgttcacgct gaccgaagcc gcgccccgac catcgcccat cttgcaggcg
     1081 cggcctgacg ctaccggcgg gctgtacaga ggctgacgga gtctgttaga ctacggaggg
     1141 acaggcgccc tcgtagctca atccggcaga gcacctcact tgtaatgagg gggttaccgg
     1201 ttcaagtccg gtcgagggct ccagcgcttg tcctgcaagg ttttccgagg atcgtgggag
     1261 ctttgtcagg ggccaaaccg gcccgaaaat gaggtgctca ccccgcccgc ccggcgtttc
     1321 ccggggcatt acggttggca cgacgggacg cgcaggtgcg gccggtgcgt gagacgattc
     1381 agagcaccac gctgagtgat gcggtcggcg cctttctcct cgcgaagcgg gtcgcgaact
     1441 gctcctcgcg caccatcggg aaccacaccg acgtgctcgc acggttccgg cgtgagtccc
     1501 agctcacgca gatccacgag gcgaccgtgg tgacgatcca aaagtacctg acggt
//
LOCUS       DASQGH010000464         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33251, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000464 DASQGH010000000
VERSION     DASQGH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33251"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="VEW91_09140"
     CDS             complement(<1..1743)
                     /locus_tag="VEW91_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HYM91783.1"
                     /translation="MSRLLRVNMTDLRATFEDVPEVYQRWGGRGLTSMVTAREVPPSC
                     HPLGPNNKLVIAPGIVSGTPAPTSGRTSFGGKSPLTGTIKESNAGGLSSQQIARLGIR
                     AIVVEGQPPDASQYWLLKLTKDGAEFLPATDLVGKGMYEVCRLLWERHGKGVAIIGVG
                     PAGEHRMAGAGICVNDMENTAGRYAGRGGMGAVMGSKGLKAIIIDDTGGPGTAIADRE
                     RFRTAIKKLTDASLKHDVTKQGGALNTYGTNALSSILNEAGGYPTRNFRTGRFELTSQ
                     ISGEAAHEIIQKRGGAGKTGHPCHPGCIIQCSDVYPRPDGTFHTSAMEYESVWALGAN
                     CGIGNLDDIADLVWACNDVGLDTIEMGGAIAVAMEGGLLKFGDAEGAKRLLNEEARHG
                     TPLGRIIGNGTEFTGRAFGVVRVPVVKGQSMPAYEPRVVKGIGVTYATSTMGADHTAG
                     YTIAPEILGVGGKADPFKPEKGELSRAFQASTAFIDTTGYCLFDAFAVLDIPEGLEGM
                     VESCAAVLGVNWTVNDIVPLGLEVLKAERAFNEAAGFTKAHDRLPEFMKREPLPPHNT
                     VIDVPDEELDKVHTY"
     gene            complement(1854..2741)
                     /locus_tag="VEW91_09145"
     CDS             complement(1854..2741)
                     /locus_tag="VEW91_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91784.1"
                     /translation="MAVIATQLAVTRETFRLDVETVSADATGTAIVGPNGAGKTTLLL
                     ALQGLLACRGRVERPTRSAGVFSQPAFLRGSARWNVSVVLQSALAMDAGAAGQRALTA
                     LRLVGLEDAADQDARRLSAGQRQRLALARALVLEPQALLLDEPFASIDADGRCALRQV
                     VGDYVARTGCALVLATQTLADVTALCRHVVLLERGRIVERLSAPGLTVSRHPYLRALL
                     SEGRFMVSWAAADQDEPAYRAPGDGPRAPIRPPKPFRLERHNDRAGAVMATGSTAGTP
                     DIGSPPDPAGRRGRVRSSQ"
     gene            complement(2732..3361)
                     /locus_tag="VEW91_09150"
     CDS             complement(2732..3361)
                     /locus_tag="VEW91_09150"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91785.1"
                     /translation="MIRIASLSVGVSLTATLIAAVFALAGALWIAYGRSPLRYALLGL
                     FNAGLGLPPVAVGLVIACAFWRSGPFGSFDILYTPTVMILAQTIIVTPLFGSLAVIAL
                     RAVDVTLRDQLAALGVPPRTRLHLLARETAPGILASALAGFGRAIAEVGAAQITGGNI
                     EGQTRVLTTATMLSVGKGEFAVAFGYVAVLLGIMALATGAAFVLQTRWR"
     gene            complement(3374..>3907)
                     /locus_tag="VEW91_09155"
     CDS             complement(3374..>3907)
                     /locus_tag="VEW91_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYM91786.1"
                     /translation="ILVMHNMFIVVAPTNDPAKVKGAGTAVEAFKRIAAAGAPFVSRA
                     DDSGTNKKELDLWGAAGVTPHGAWYIRSGSGMADTLHIASQKAAYTLTDDGTFLSQRA
                     TLALVPVVEDAKDLRNIYHVIVVRPIAGHVANLAGGEAFARYIVSAEGQHIIATFGKA
                     KYGRPLFVADAGTAAAH"
BASE COUNT          621 a         1341 c         1294 g          651 t
ORIGIN      
        1 ataggtgtgc accttgtcca gctcctcgtc cggcacatcg atgacggtgt tatgtggcgg
       61 gagcggctcg cgcttcatga attccggcag gcgatcatgg gccttggtga atccggccgc
      121 ctcgttgaac gcgcgctcgg ccttcaggac ttccaggccg agcggcacga tgtcgttcac
      181 cgtccagttc acgcccagca cggccgcaca ggattcgacc atcccctcca gcccctcggg
      241 gatgtccagc acggcgaagg cgtcgaacag gcagtagccg gtcgtgtcga tgaacgccgt
      301 ggaggcctgg aacgcgcggg acaactcccc cttctcgggc ttgaacgggt cggctttgcc
      361 gccaacaccc aggatttccg gggcgatcgt gtagcccgcc gtgtgatcgg cgcccatcgt
      421 gctcgtcgcg tacgtgacgc cgatcccctt gaccacccgg ggctcgtacg cgggcatcga
      481 ctgccccttg accaccggca cgcggacgac gccgaacgcc cggcccgtaa actcggtccc
      541 gttgccgatg atgcgtccta ggggagtacc gtgccgcgcc tcctcgttga gcaaccgctt
      601 ggcgccctcg gcatcgccga acttgagcag gccgccctcc atggccaccg cgatggcgcc
      661 gcccatctcg atcgtatcca gcccaacgtc gttgcacgcc cagaccaggt cggcgatatc
      721 gtccaggttg ccgatcccgc agttggctcc aagcgcccac accgactcgt actccatcgc
      781 cgaggtgtgg aacgtcccgt ccggacgggg atagacgtcg gagcactgga tgatgcaccc
      841 cgggtggcat ggatggccgg tcttgccagc tcccccgcgt ttttggatga tctcgtgggc
      901 ggcttcgccg ctgatctggc tggtgagctc gaaccggccg gtgcggaagt tccgggtggg
      961 ataccctccc gcttcgttca agatgctgga cagcgcgttt gtcccgtagg tgttcagcgc
     1021 gccgccctgc ttggtcacgt cgtgcttcaa tgacgcgtcc gtgagtttct tgatggctgt
     1081 ccggaagcgc tcgcggtcgg cgatcgccgt tcccgggccg ccggtgtcgt cgatgatgat
     1141 ggcctttagc cccttcgagc ccattaccgc gcccatcccg ccgcggccgg cgtagcggcc
     1201 ggcggtgttc tccatgtcgt tcacgcagat gccggccccg gccatccggt gctccccggc
     1261 cggtccgacg ccgatgatcg cgacaccctt cccgtggcgt tcccacagga ggcggcagac
     1321 ctcgtacatc cccttgccga ccaggtccgt ggcgggaagg aactcggctc cgtccttggt
     1381 cagcttgagc agccagtact ggctggcgtc cggaggctgc ccctcgacga cgatggctcg
     1441 aatgccgagg cgggcgatct gttgagacga cagccctccg gcgttgctct ctttgatcgt
     1501 gccagtcagg ggactcttgc cgccgaacga ggtccggcca gaggtcggcg ccggggtgcc
     1561 gctgacgatg ccgggcgcga tgacaagctt gttgtttggg ccgagagggt ggcaggaggg
     1621 cgggacttcc cgcgccgtca ccatggaggt caggccccgt ccgccccacc tctgatagac
     1681 ttcggggaca tcctcaaagg tggctctcag gtcggtcatg ttcacccgca gcagcctgct
     1741 catcggactt tcctccgact gccagatgtg cccttgccgc gctcacaagg ccgcgcaggc
     1801 cgattgacct cccggctgcg cactgctaaa gcccattgag gaaccatacg ctcttattgg
     1861 ctagatctta ccctcccccg ccggccagcg ggatcgggcg gagacccgat atcgggagtc
     1921 ccggcggtcg agccagtggc catgaccgcc cccgcccgat cgttgtgcct ttcgagccgg
     1981 aatggcttag ggggccggat tggagcgcgg ggcccatcgc ctggagcgcg gtacgcgggc
     2041 tcgtcctgat ccgcggcggc ccacgatacc atgaagcgac cctccgagag gagcgcccgc
     2101 agatacgggt gtctactgac cgtgagcccc ggcgcgctga ggcgctcgac gatgcggccg
     2161 cgctcgagca agacgacgtg gcggcacaac gccgtgacgt cggcgagtgt ctgggtggcg
     2221 agaaccaacg cgcagccggt gcgtgcgaca tagtcgccga caacctgtcg gagggcgcac
     2281 cgcccgtccg cgtcgatgct ggcaaagggc tcatcgagca ggagggcctg cggttcgagg
     2341 accagggccc tggcaagcgc gagccgctga cgctgtcccg cggaaagccg ccgcgcgtcc
     2401 tggtccgccg cgtcctcgag gccgaccagg cgaagcgccg tgagagcccg ctgtccggcg
     2461 gccccggcgt ccatcgccaa cgccgattgc aagacgaccg aaacgttcca acgtgctgag
     2521 ccgcgcagaa acgcgggctg cgagaagacc cccgcgctgc gcgtgggtcg ttcgacgcgc
     2581 ccgcgacatg caaggagccc ctgcagcgcc aggagcaagg tcgttttccc ggccccgttc
     2641 ggcccgacga tcgccgtccc ggttgcatcc gcggagaccg tctcgacgtc gagccggaac
     2701 gtttcgcgcg tgacggccag ctgagttgcg atcaccgcca tcgcgtttgc aacacgaagg
     2761 ccgcccctgt cgcgagcgcc atgatcccga gcagcacggc gacgtagccg aaggcgacgg
     2821 cgaactcgcc cttgccgaca ctgagcatcg tcgcggtcgt aagcacacgg gtctggccct
     2881 cgatgttgcc ccccgttatc tgagcggcgc cgacctccgc gatggcccgc ccgaagccgg
     2941 ccagtgccga cgccaggatc ccgggcgccg tctcgcgcgc caggaggtgc aggcgcgtgc
     3001 gcgggggcac gcccaacgcc gcaagctgat cgcgcaacgt cacgtcgacc gcgcgcaagg
     3061 cgatgaccgc cagtgaaccg aagagcggcg tcacgatgat tgtttgggcg agaatcatca
     3121 ccgttggcgt gtagaggatg tcgaacgacc cgaacggtcc gctccgccag aacgcgcatg
     3181 cgatcaccag ccccacggcg accggcggaa gccccaaccc ggcgttaaag agcccgagga
     3241 gcgcgtaacg cagcggggag cgtccgtagg cgatccagag ggcgccggct aacgcgaaga
     3301 ccgccgcgat cagcgtcgct gtgagcgaga cgccgaccga cagcgacgca atccggatca
     3361 cggcgctcat cgtctagtgc gcggccgcgg tcccggcgtc ggcgacaaac aaggggcggc
     3421 cgtacttggc tttgccgaac gtagcgatga tatgctgtcc ttctgccgat acgatgtacc
     3481 tggcaaacgc ttcaccgccc gcgagatttg cgacgtgacc ggcgatcggt ctcaccacga
     3541 tgacgtggta gatgttgcgg agatccttgg catcttcgac gactggcacg agcgccaggg
     3601 tcgcgcgctg cgagaggaac gtcccatcat cggtgagcgt gtaagcggcc ttctgcgagg
     3661 cgatatgcag cgtatccgcc atcccgctgc ccgacctgat gtaccaggcg ccgtggggcg
     3721 tgaccccggc ggctccccag aggtcgagct ccttcttgtt tgtccccgag tcatcggcgc
     3781 gcgagacgaa cggcgcgccg gcggcggcga tccgtttgaa cgcctcgacg gcggtcccgg
     3841 cccctttgac cttcgcgggg tcgtttgtcg gggccacgac gatgaacatg ttgtgcatga
     3901 cgagtat
//
LOCUS       DASQGH010000465         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41663, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000465 DASQGH010000000
VERSION     DASQGH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41663"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VEW91_09160"
     CDS             <1..378
                     /locus_tag="VEW91_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HYM91787.1"
                     /translation="RCRWLRENAFDGLRRLAASGPAFDLVILDPPAFVKTRSALATGL
                     AGYKEINLRALKVLGPGGWLVTCSCSYHVDEAQLTAVVHEAARDARRRIRIVERRSQA
                     GDHPVHPAMPETRYLKCLIIAVE"
     gene            588..2117
                     /locus_tag="VEW91_09165"
     CDS             588..2117
                     /locus_tag="VEW91_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HYM91788.1"
                     /translation="MVGHQIRITDNLDVLLGVLPPQIRAAVEQQPDLDELLEVVLDLG
                     REPEARFPGRAVRLTDGFVRSDEMQYVVSRVGTFGKDNRAGIERTLHRISAIRNRAGE
                     IIGLTCRVGRAVYGTVDIVRDVVESGQSVLLVGRPGVGKTTLLREAARVLSDEIAKRV
                     VVVDTSNEIAGDGDIPHPGIGRARRMQVPVPELQHAVMIEAVENHMPEVIVIDEIGTE
                     AEALAARTIAERGVQLIATAHGNTLDNLLQNPTLCDLVGGIQAVTLGDEEARRRGTQK
                     TVLARKAPPTFEVVIEIQEQDRLAVHHDVAHVVDRFLRGHVPRAEIRTRTPEGEVRIS
                     TNGEPLGAPSPAADGHPPAARPPHKIVRIYPYAVSRNRLERAIRELRVPAEIANTLSE
                     ADLLLTLKSQEKRQPKRLREAGTRGLAMHVIKSNTVSQIEAMLRGIFSVEDRQSPDEV
                     AAREVEEAISEVMASAQPVELSPQNSYIRRLQHQLIQRYGLSSESKGIDPYRRVVIYP
                     H"
     gene            2107..>2310
                     /locus_tag="VEW91_09170"
     CDS             2107..>2310
                     /locus_tag="VEW91_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYM91789.1"
                     /translation="MRTEARPAPAPGLLLTLEGPDGAGKTTQSRLLADYLRSGGREVV
                     SVREPGGTAIGEQIRSLLLDPRWE"
BASE COUNT          357 a          774 c          857 g          322 t
ORIGIN      
        1 cgctgccggt ggctccggga gaacgccttc gatgggctgc ggcggctggc ggcgtccggt
       61 cccgcgttcg acctggtgat cctggatccg cccgcgttcg tgaagacccg gtccgccctc
      121 gccaccgggc tggcgggata caaggagatc aacctccgcg cgctcaaggt gctgggtccc
      181 ggtggctggc tcgtgacgtg ctcgtgctcg tatcacgtgg acgaagcgca gttgaccgcc
      241 gtcgtgcacg aggccgcgcg cgatgcgcgg cggaggatca ggatcgtcga acgccgttcc
      301 caggccgggg atcatccggt gcaccccgcg atgccggaga cccggtacct gaagtgcctg
      361 atcatcgccg tggagtgagg gggatgcgcg gccgggcctg gcggcgggcg gcccccgcgg
      421 ccggttgcgg gcgggctcgc cgtgtctcta taatggaagc ggagatgttt cggtcgcagc
      481 atagcgtggg ggcggcgcgg gggagagccg ggggccgggt tctcccccgt ggtgtttctg
      541 ccccgcgccc cttgccgtcg aagagaacga agccgaggtg atcgtgggtg gtcggccacc
      601 agatccgcat caccgataac ctggacgtgc tgttgggggt cctgcctccg cagatccgcg
      661 cggccgtcga gcagcagccg gacctggatg agctcctgga ggtggtgctc gatctgggcc
      721 gcgagcccga agcccggttc cccggccggg cggtgcggtt gacggacggg ttcgtgcgga
      781 gcgacgagat gcagtacgtg gtcagccggg tcggcacgtt cgggaaggac aaccgcgccg
      841 ggatcgagcg gacgctccac cggatctcgg cgatccgcaa ccgggcgggc gagatcatcg
      901 ggctgacctg ccgggtgggc cgggcggtat acggcacggt cgacatcgtg cgggacgtgg
      961 tcgagtcggg gcagagcgtc ctgctggtcg gacggccggg ggtcggcaag accaccctcc
     1021 tccgggaggc cgcccgtgtc ctctccgatg agatcgccaa gcgggtggtc gtggtggata
     1081 cgagcaacga gatcgccggc gacggcgaca tcccccaccc ggggatcggg cgcgcgcgcc
     1141 gcatgcaggt gccggtcccg gaactccagc acgcggtgat gatcgaggcg gtggaaaacc
     1201 acatgccgga ggtgatcgtc atcgacgaga tcggcaccga agcggaggcg ctggctgccc
     1261 gcacgatcgc ggagcggggc gtgcagctca tcgccacggc gcacggcaac accctcgaca
     1321 acctcctgca gaaccccacc ctgtgcgacc tcgtcggggg gatccaggcg gtcacgctgg
     1381 gcgatgagga agcccgccgg cggggcacgc agaagaccgt gctggcgcgc aaggccccac
     1441 ccacgtttga ggtcgtcatc gagattcagg agcaggatcg gctggcggtg caccacgacg
     1501 tcgcgcacgt cgtggaccgg tttctgcggg ggcacgtgcc gcgcgccgag atccggacgc
     1561 ggacccccga aggcgaagtg cggatcagca ccaacggcga gccgctgggt gccccctcgc
     1621 cggcggccga cggccacccg cccgccgcga ggccgccgca caagatcgtg cgcatctacc
     1681 cctatgccgt gagtcggaac cggctcgagc gcgcgattcg cgagctgcgc gtgccggccg
     1741 agatcgccaa caccctgtcc gaggccgacc tgctgctcac cctgaagtcg caggagaaac
     1801 ggcagcccaa gcggctccgc gaggccggga cccgcgggct ggccatgcac gtcatcaaga
     1861 gcaacaccgt ctcccagatc gaggcgatgc tccggggcat cttcagcgtc gaagaccgcc
     1921 agagccccga cgaggtggcg gcgcgcgagg tcgaggaggc gatctcggag gtgatggcct
     1981 ccgcgcagcc ggtggagctc tccccccaga actcatacat ccgccggctg cagcaccagc
     2041 tcatccaacg ctacggcttg agttccgaga gcaagggcat cgatccatac cggcgtgtgg
     2101 tgatctatcc gcactgaagc gcgccctgcg cccgcccccg ggctcctcct cacgctggag
     2161 gggcccgacg gcgccgggaa gacgacccag agccggctgc tggccgacta cctccggagc
     2221 ggcggccggg aggtggtgtc cgttcgtgag cccgggggca cggcaatcgg cgagcagatc
     2281 cggtcgctcc tgctggatcc tcggtgggag
//
LOCUS       DASQGH010000466         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19055, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000466 DASQGH010000000
VERSION     DASQGH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19055"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..689
                     /locus_tag="VEW91_09175"
     CDS             378..689
                     /locus_tag="VEW91_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HYM91790.1"
                     /translation="MLTEIDVRATLRQKLNAEFREYVILGACNPPLAHRALEADLDVG
                     LLLPCNVVVYQDGPGSVVAILDPIAMLGVSDNAALRPIAAEAKVRLERVIQRLQSQQA
                     L"
     gene            873..3203
                     /locus_tag="VEW91_09180"
     CDS             873..3203
                     /locus_tag="VEW91_09180"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYM91791.1"
                     /translation="MTAATTTAHVDPVCGMVVEPDTPHRIDHAGTSYLFCSAGCVATF
                     RQDPARYSGVSHPAHERDGKGPGSTAGTSVAYTCSMHPEIVRDAPGTCPICGMALEPR
                     GVAEEEANPELVDMTRRFWISVVLTAPLAIIAMNDLLPGRPLERFASFRGLGWLELAL
                     ATPVVLWGGWPFFVRAWQSVLNRSLNMFTLIGLGVAVAYAYSLVGTVAPGLFPASFRD
                     GAGTVPVYFEAAAVIVTLVLLGQVLELRARSQTGAAIRALLGLAPKTARCVHADGAEE
                     DVPLVQVRPGDQLRVRPGEKIPVDGVVVQGTSAVDESMVSGEPIPVEKRPGDRVIGAT
                     VNGTGMLLMRAERVGAETLLARIVQMVAEAQRSRAPIQKLADVVAGYFVPAVTAVAVI
                     TFIVWGLVGPEPRLAHALVNAVAVLIIACPCALGLATPMSIMVATGKGATMGVLFRNA
                     EAIEVMRKVDTLVVDKTGTLTEGKPKLVTVAPADGWDEATLLRLAASLERGSEHPLAA
                     AIVAGARTRDVTLTNVDAFESRTGKGVAGRVDGRFVALGTRMLLEELGVHPDGLAGRA
                     ESLRADGETVMFVVVDGAAAGLIGVTDPIKETTPEAIRQLHREGLRIVMLTGDSRTTA
                     EAVAKKLGLDAVVAEVLPDQKVDVVKRLQDEGRAVAMAGDGINDAPALAQAQVGIAMG
                     TGTDVAMESAGVTLVKGDLRGIVRARRLSRATMRNIRQNLFFAFAYNALGIPIAAGAL
                     YPVFGLLLSPMIAAAAMSFSSVSVVGNALRLRRVRV"
     gene            3271..3516
                     /locus_tag="VEW91_09185"
     CDS             3271..3516
                     /locus_tag="VEW91_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HYM91792.1"
                     /translation="MTVVVYGKPDCSLCEKAIAILERLQREFGFHIEHVDITRDPGLF
                     IRYRERVPVVVLDGQEVAWGIVTIPGLRSALGRLLQK"
BASE COUNT          583 a         1106 c         1264 g          601 t
ORIGIN      
        1 gcgtggtgtc aacgacaagt cgcagaagcg cggtagcccg cgtgacgttg cgcctgacgc
       61 gatcaatcgc gctgccgcca caagcgcgca caccaccgcc tttgaccgcc tccggcagct
      121 cctctttgtg ttcctcccaa acagccgcca cgcggctgtg tacgaggaga agtagcccca
      181 tcgcgaggaa ttgcgagtga gctgtagcga aacgggccgc ccggccgggg gcgagtgcat
      241 cggatgagtt cttcaaggga gggagaaaga ccatggtgca gagagcaacg cgatacggga
      301 catcggtgag gctccccgtg acccacgacc aagccgtggc acgagtaagg gaagccctga
      361 aagaagaagg atttggggtg ctgaccgaga tcgacgtgcg ggcaacgttg aggcaaaagc
      421 tgaacgcgga gtttcgggaa tatgtgattc tgggcgcgtg caacccgccg ttggcccatc
      481 gcgcgctcga ggcggatctc gatgtgggcc tgctgctccc gtgcaacgtg gtcgtgtacc
      541 aagacggtcc ggggtcggtc gtcgcgatcc tcgatccaat cgccatgctg ggtgtcagcg
      601 ataacgcggc actccgaccg atcgcggctg aggcgaaggt tcgcctcgag cgggtgatcc
      661 aacgcttgca aagccaacag gcgctgtagc gacaccggct gagcccgccg ctgggaagcg
      721 aaacgtaagc gggatccgtt ttgctacacg gggagagtag tatgcgccct gcacccggcc
      781 cattcgccgg gatccgggtt agcgtgcgcc tctccgggag aaccgcgatg cgcgccgagc
      841 atgagccgcg cggccaggtc tatggggagg acgtgacagc ggcgacgact acagcgcacg
      901 tcgatcccgt gtgcggtatg gtggtcgagc cggacactcc ccaccggatc gatcacgctg
      961 ggacgtccta cttgttctgc agtgctggtt gcgtggccac gttccgccag gatccggcac
     1021 gctacagtgg tgtttctcat ccagctcatg aacgcgatgg caaagggccg ggcagcaccg
     1081 ccgggacgtc cgttgcgtat acttgctcaa tgcaccctga gatcgtccgc gacgcgcccg
     1141 gcacctgccc catctgcggg atggcgctgg aaccccgggg cgtggctgag gaggaagcga
     1201 accccgagct ggtcgacatg acgcggcgct tctggattag tgtggtcctg acggctccgc
     1261 tcgccatcat cgccatgaac gacctcctgc ccggccggcc gctcgaacgg ttcgcctcct
     1321 ttcggggctt ggggtggctc gagctcgcgc tcgccacccc ggtggtcctc tggggcggat
     1381 ggccgttctt cgtgcgggcc tggcagtcgg tcctgaaccg tagcctcaac atgttcacgt
     1441 tgatcgggct tggggtcgcc gtcgcctacg cctacagcct cgtgggaacc gttgctccag
     1501 gcctcttccc ggcatcgttc cgggatgggg caggcaccgt gccggtctac tttgaggccg
     1561 cggccgtgat cgtgacgttg gtcttgctgg gacaggtgct ggagctgaga gcccgaagcc
     1621 agaccggtgc ggccatcaga gccctgctcg gcctggcccc gaagacggcc cgctgcgtcc
     1681 acgcggatgg cgccgaggag gacgtcccgc tcgtccaggt gcgccccggc gaccagctcc
     1741 gtgtgcgccc cggcgagaag atccccgtcg atggcgtcgt cgtccagggg accagcgcgg
     1801 tggacgagtc catggtgtcg ggcgagccca tcccggtgga gaagcggccg ggcgaccggg
     1861 tgattggcgc caccgtgaac gggaccggca tgctgctgat gcgggccgaa cgcgtcggcg
     1921 ccgagaccct cctggcgcgg atcgtccaga tggtggcgga agcgcagcga agccgcgcac
     1981 cgatccaaaa gctggccgat gtcgtggcgg ggtactttgt gccggcggtc acggcggtgg
     2041 ccgttatcac gttcatcgtg tggggcctgg tcggcccgga gccgcggctg gcccacgccc
     2101 tcgtcaacgc cgtcgcggtg ctcatcatcg cctgcccgtg cgcgctcggg ctcgcgaccc
     2161 cgatgtccat catggtggcc accggcaaag gggcgacgat gggggtgctg ttcaggaacg
     2221 ccgaggcgat cgaggtgatg cggaaagtcg acacgctcgt cgtggacaag accgggacgc
     2281 tcaccgaggg gaagccgaag ctggtcaccg tggcgcccgc ggacggctgg gacgaggcga
     2341 cgctcctgcg cctcgccgcg agcctcgagc ggggcagcga acatccgctg gcggcggcga
     2401 tcgtagcggg tgcgcgaacg cgggatgtta cgctcacgaa cgtcgatgcg tttgaatcga
     2461 gaaccggcaa aggggtggcg ggccgggtcg acggccggtt cgtcgcgctc ggcaccagga
     2521 tgttgctcga agagctgggc gtgcacccgg acggcttggc cggaagggcc gagtccctgc
     2581 gggccgacgg cgaaaccgtg atgttcgtgg tagtggatgg cgccgcagcc gggctcatcg
     2641 gcgtgaccga tcccatcaag gagaccacgc ccgaggccat ccggcagctc caccgcgagg
     2701 gcctccgcat cgtcatgctt accggagata gccggacgac cgccgaggcc gtggccaaga
     2761 aattgggcct tgacgcggtc gtggccgagg tcttgccgga ccagaaggtc gacgtggtca
     2821 agcgcctgca ggacgaggga cgcgcggtgg ccatggcggg tgacggcatc aacgatgccc
     2881 ccgcgctggc gcaggcgcag gtggggatcg cgatggggac ggggaccgac gtggcgatgg
     2941 agagtgccgg ggtgacgctg gtcaagggcg acctgcgcgg gatcgttcgg gcacggcggc
     3001 tgagccgggc gacgatgcgc aatattcggc aaaacctctt cttcgctttc gcctacaacg
     3061 ccctcggcat cccgatcgcg gcgggggccc tctacccggt cttcggcctg ttgctcagcc
     3121 cgatgatcgc cgccgcagca atgagcttca gttcggtctc ggtcgtcggg aacgcgctcc
     3181 ggttgcgtcg tgtgcgcgtc tgagagacca tgcgaacgga acagggtccc atcggcgagt
     3241 cttgcccgcg atgactgagc cggaggtccc atgacagtcg tggtgtacgg gaaaccggat
     3301 tgctccctct gcgagaaggc gatcgccatt ctggagcgtc tccaacgaga attcggtttt
     3361 cacatcgagc acgtcgacat cacgcgggat cccgggctct tcatccgata ccgggagagg
     3421 gtcccggttg tggttctgga cggccaagag gtcgcctggg gcattgtgac gattcctggc
     3481 ctacggtccg ccctgggtcg tttgttgcag aaatgatcgg gtcttggcct ccatgatttc
     3541 tccatagact gttg
//
LOCUS       DASQGH010000467         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19068, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000467 DASQGH010000000
VERSION     DASQGH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19068"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>1509
                     /gene="purF"
                     /locus_tag="VEW91_09190"
     CDS             175..>1509
                     /gene="purF"
                     /locus_tag="VEW91_09190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYM91793.1"
                     /translation="MMPGGDKLREECGVFGIRTAAAPAAPYVHLGLYALQHRGQESAG
                     IATFDGKQTHLVKDMGLVGGIFTPERLAGLRGAVGIGHVRYSTMGSVTLENAQPFVEP
                     TPWGVLALAHNGNLINAPALRRELESAGHCFRATSDTEVLTKLIATSKAQTLESAIAR
                     CMRRISGAYTIVAMAGDRLVAFRDANGIRPLALGRVEGAVVVASESCAFDQIGGEYVR
                     DVEPGELLVADAAGLRSIQVLPVVRRAACVFEYIYFARPDTVLGDQNVHQVRRRMGRL
                     LAREHPAAADIVVAVPDSGTSAALGYSDESGIACEIGLIKNRYVGRTFIQPDPSSRDF
                     GVRVKLNPIREVVRGRRVVLIDDSIVRGTTSHQIVRMLRHAGAREVHVRISSPPILNA
                     CYYGIDTSSRGELVASRLSVEEIRRSIEADTLGYLSLDGLVQALALPQKDLCL"
BASE COUNT          219 a          511 c          545 g          234 t
ORIGIN      
        1 ccttcccgcg ctgctccgcc tggccagccg gctggccgtt ccggtccagg tgctggggac
       61 ggtcgggggg gagcgtctta tcgtcggggt ggatggggac gctgcccgcg gaccatggat
      121 cgacgccggc gtggacgacc tggcccgcgc ctggcagggg aaaggggccc atcaatgatg
      181 cccggcgggg acaaactgcg cgaggagtgc ggggtcttcg gcatccggac agcggcggcc
      241 ccggccgcgc cgtacgtgca cctgggactg tacgcccttc agcaccgcgg ccaagaaagc
      301 gccggcatcg ccacgttcga tgggaagcag acgcacctgg tgaaggacat ggggctcgtg
      361 ggggggatct tcaccccgga gcggttggcc gggctccgag gtgcggtggg gatcggccac
      421 gtccggtatt cgacgatggg ctcggtcacg ctcgagaacg cccagccctt cgtcgagccc
      481 acgccgtggg gcgtcctcgc gctcgcgcac aatggcaacc tgatcaacgc cccggcgctc
      541 cggcgggaac tcgaatcggc cggccactgc ttccgggcca cctccgacac cgaggtgctc
      601 accaaactca tcgccacatc gaaggcgcag acgctggaaa gcgcgatcgc gcgctgcatg
      661 cgccggatca gcggcgcgta cacgatcgtg gccatggcgg gcgaccgcct ggtggcgttt
      721 cgggatgcga acggcatccg cccgctcgct ctcggccggg tggagggcgc ggtcgtggtc
      781 gcctcggaga gctgcgcgtt cgaccagatc gggggcgagt acgttcgcga tgtcgaaccc
      841 ggcgagttgc tcgtcgcgga tgctgcgggc ctccgctcga tccaggtgct gccggtggtg
      901 cgtcgggccg cctgcgtttt cgagtacatt tatttcgccc ggccggacac cgtgctcggc
      961 gaccagaacg tgcaccaggt ccggcgccga atgggccgtc tgctcgcgcg cgagcacccc
     1021 gccgcggccg atatcgtggt cgcggtcccg gactcgggca cctccgccgc attgggctac
     1081 tcggacgaga gcgggattgc ctgcgagatc ggattgatca agaaccggta cgtgggacgc
     1141 acgttcatcc agccggaccc atcgtcgcgg gacttcggcg tccgcgtcaa gctcaacccc
     1201 atccgggagg tcgtgcgggg gcgccgcgtg gtgctgatcg acgattcgat cgtccggggc
     1261 acgacgagcc accagatcgt gcgcatgctg cgacatgcgg gcgcccgcga ggtgcacgtc
     1321 cggatctcct cgccgccgat cctcaacgcc tgctactacg gcatcgacac cagcagccgg
     1381 ggggaactcg tggcctcccg gctctcggtc gaggagatcc ggcggtcgat cgaggccgat
     1441 accctcgggt acctgagcct cgacgggctg gtccaggcgc tggcgctgcc gcagaaggac
     1501 ctgtgcctc
//
LOCUS       DASQGH010000468         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47401, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000468 DASQGH010000000
VERSION     DASQGH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47401"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VEW91_09195"
     CDS             <1..209
                     /locus_tag="VEW91_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91794.1"
                     /translation="ALRPVAWALPPTHAFEGMRTALSGGGIAWDRVVWGVAENAVLLA
                     GAAIIFQWVMHVARDRGLLLRVEG"
     gene            287..547
                     /locus_tag="VEW91_09200"
                     /pseudo
     CDS             287..547
                     /locus_tag="VEW91_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            657..1130
                     /locus_tag="VEW91_09205"
     CDS             657..1130
                     /locus_tag="VEW91_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYM91795.1"
                     /translation="MQAVLVDARWPRGAVRLAEAARRRDLPVVLDFDQGTPAAWRLAA
                     LATHAVADQELAKTCGGVRPLLKRLADLGTWGGVTLGARGVAHPGGRFPSFRVTPRDT
                     TGAGDVFHGAFALALAEGRGEEAALAFASAAAAQRCALAEVPRRDDVIRLLNGRR"
     gene            1127..1858
                     /locus_tag="VEW91_09210"
     CDS             1127..1858
                     /locus_tag="VEW91_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYM91796.1"
                     /translation="MSAGAAQAPGPGVLAGTSGFAFAEWKGGFYPEDLKGNRMLAFYG
                     ERLPTVEINVSFYRMPTAKMLEDWKAQTPPPFRFAIKAHRRITHQKRLHDVDEDVRWL
                     CERVAELGERLGPVLFQLPPSLKADLGLLENFLATLRPLPYVAMEFRHQTWHQDATYE
                     LLRRYRVALCIAEDEQSCEPLVHTAPFGYYRLHRLRYTEAQLESWADHLRSAAQIRPA
                     FCYFTHETGPEAVAYALRLMDLMRG"
     gene            complement(1855..>3005)
                     /locus_tag="VEW91_09215"
     CDS             complement(1855..>3005)
                     /locus_tag="VEW91_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYM91797.1"
                     /translation="KRGTGLSDPVVELPTLEQRMDDVRAVMDAVGSERAALFGLSEGG
                     SMCALFAATYPKRTSALILCGAFAKRIWSPDYPWAPTPEERQRFFDAIETGWGGIVDL
                     KTLAPSAADDPQFRDWWSTYLRLGASPGAALAHARMNTEIDIRHVLGSIRVPALVLHR
                     SGDLDAKVEEGRYIADRIPGAKFVELAGRDHLPFVGDADALLDEVEEFLTGVRTSPEA
                     ERILATVLFADIVGSTKRAAELGDRRWRDLLEAFYALIRRNLAAFRGREIDTAGDGVL
                     ATFDGPARAIRCACAIRDAARELGIEVRTGLHTGECEVIGPKLGGIAVHIGARVASHA
                     GAGEVLVSSTVKDLVAGSGLRFEDRGAHALKGVEGEWRLFAVAEVTAA"
BASE COUNT          423 a         1065 c         1063 g          454 t
ORIGIN      
        1 ccgccctccg accggtcgcc tgggcgcttc cgccgacgca cgcgttcgaa gggatgcgca
       61 ccgcgctgag cggcggcggc atcgcctggg accgggtggt gtggggggtg gcggaaaacg
      121 cggtgctcct cgcgggcgcg gcgataatct ttcagtgggt gatgcacgtc gcccgcgacc
      181 ggggcctgct gcttcgggtg gaggggtgag gcaggcgggc cgccgggcca cgggcggccg
      241 cgcgcgcccg ggatggtcgc cgaagcgacc cgtggcccgg gtcgcggtgc tcggctacgc
      301 ctgtctggac caccggttct gggcgcagcg gttccctccg gtccgtgcgc gcaccccggt
      361 cagggcctac cgcgcggagc ttggaggcct ggcggcggtg ggcgcggtgg ccgtggtgcg
      421 cttgggcggc gcggcggtct tcttcggcag gcggggggat gacgaggccg ggcaacgagt
      481 cgaggcgagc ctccgggccg agggcgtcga gaccttcgcg ttccgggtgt ttccggggat
      541 cgccacgccc gtgagcggca ttctcatcgc gccgacgggc gagcggtaca ttttcccgta
      601 cccggggaaa gggctgccgg acgacgggct ggctgccgct gcaggtactc gagacggtgc
      661 aggcggtgct tgtcgacgcc cgctggccgc gaggcgcggt gcgcctcgcg gaagccgccc
      721 gcaggcgcga cctcccggtt gtgctcgatt tcgaccaggg aacccccgcg gcctggcggc
      781 tggcggcgct cgcgacccac gcggtggccg accaggaact cgcgaaaacg tgcgggggcg
      841 tccgacccct gcttaagcgg ctcgcggacc tcgggacctg gggtggggtc acgctgggcg
      901 ctcggggcgt ggcgcatccg gggggccgct tcccatcatt tcgcgtcacc ccgcgcgaca
      961 cgaccggcgc aggcgacgtg tttcatggag cgttcgcgct cgcgctggcc gaaggacgcg
     1021 gcgaggaggc agcgctggcg ttcgcctcag cggcggcggc gcagcggtgc gcgctcgccg
     1081 aggtcccgcg tcgcgacgac gtgatccggc tcctcaacgg acggcgatga gcgccggcgc
     1141 ggcccaggct ccgggcccag gcgtgctggc cgggacgtcg gggtttgcgt tcgccgagtg
     1201 gaagggcggg ttctatcccg aggacctcaa ggggaaccgg atgctcgcct tctacggcga
     1261 gcgcctcccc accgttgaga tcaatgtgtc cttctaccgc atgccgacgg ccaagatgct
     1321 ggaggactgg aaggcgcaga ccccgccgcc gttccgtttc gcgatcaaag cccaccggcg
     1381 gatcacccac cagaagcggc tccacgatgt ggatgaggac gtgcggtggc tctgcgagcg
     1441 cgtcgccgaa ctgggggagc ggctcgggcc cgtgctcttc cagctcccgc cgtcgctcaa
     1501 ggcggacctg gggctgctgg agaattttct tgcaacgctc cgcccgctcc cgtacgtggc
     1561 gatggagttt cgccaccaga cctggcacca ggacgccacc tacgagctcc tgcgccggta
     1621 ccgcgtcgcg ttgtgcatcg cggaggacga gcaatcctgc gagccgctgg tccacaccgc
     1681 gccgtttggg tactaccgcc tgcaccggct caggtacacc gaagcgcaac tcgagagctg
     1741 ggccgaccac ctgcggtcag ccgcgcagat ccgtccggcg ttctgctact tcacgcacga
     1801 gaccggcccc gaggcggtgg cgtacgccct ccgtctcatg gacctgatgc gcggttaggc
     1861 cgcggtcacc tcggcgaccg caaacaacct ccactccccc tcgaccccct tgagggcatg
     1921 cgcgccccgg tcctcaaagc ggaggcccga cccggccacg aggtccttga ccgtgctcga
     1981 cacgagcacc tcgcctgctc cggcgtggga ggcgacccgg gcgccgatgt gcacggcgat
     2041 tccgcccagc ttgggtccga tgacctcgca ctcccccgtg tggagtcccg tgcggacctc
     2101 gatgccgagt tcgcgggcgg catcccggat ggcgcaggcg caacgaatcg cccgggcggg
     2161 accgtcgaat gtggccagca ccccgtcgcc ggccgtatcg atctcccgcc cccgaaatgc
     2221 ggcaaggttg cgccggatca aggcgtagaa cgcttccagc agatcgcgcc acctgcggtc
     2281 gcccagttcg gcggcccgct tcgtcgatcc cacgatatcg gcaaacaaca cggtcgccag
     2341 gatccgctca gcctcgggcg atgtcctgac accggtgaga aactcctcga cttcgtccaa
     2401 gagcgcatcc gcgtccccga cgaaggggag gtggtcgcgc cccgccagct ccacgaactt
     2461 ggcccccggg atccggtcgg cgatgtatcg gccttcctcc accttcgcat ccaggtcgcc
     2521 gctccggtgg agcaccagcg ccgggacacg gatgctcccc agcacgtgcc ggatatcgat
     2581 ctccgtattc atccgggcgt gcgccagggc ggctcccgga cttgccccga gccgcagata
     2641 ggtggaccac cagtctcgga attgtggatc gtcagcggcg ctcggggcca gggtcttcag
     2701 gtcgacgatc ccgccccacc cggtctcgat ggcgtcaaag aaccgttggc gctcttccgg
     2761 cgtcggggcc caggggtagt ccggcgacca gatcctcttc gcgaacgcgc cacataggat
     2821 cagcgccgag gtccgcttgg gatacgtggc ggcaaacagc gcacacatcg accccccctc
     2881 ggacagccca aagagcgctg cccgctcaga gcccaccgca tccatgacgg cgcgcacgtc
     2941 gtccatgcgc tgttccaggg tcgggagttc cacgaccggg tcggacaggc cggtcccgcg
     3001 cttat
//
LOCUS       DASQGH010000469         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50162, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000469 DASQGH010000000
VERSION     DASQGH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50162"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VEW91_09220"
     CDS             <1..561
                     /locus_tag="VEW91_09220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYM91798.1"
                     /translation="PHDWPRLPPNLPVPQDDGVCDHLPGRRLPAISLPSTAGRLVELA
                     AVPGIAVVYCYPRTGRPDIPSPKGWDAIPGARGCTPQACAFRDHHGEFLALGARVFGL
                     STQTTEDQREATERLHLPMELLSDAGLAFAHALQLPTFEVEGMVLIKRLTLVARNGHI
                     TKVFYPIFPPDANAQEVLTWLRALPR"
     gene            537..1406
                     /gene="sseA"
                     /locus_tag="VEW91_09225"
     CDS             537..1406
                     /gene="sseA"
                     /locus_tag="VEW91_09225"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HYM91799.1"
                     /translation="MAAGTPTMSASPLDPAVSPAWVAAHLAEPDVRVVDATWYVPWLH
                     RDAQREFREAHIPGAAYFDIDAIADRRTTLPHMLPDAESFAQSVGALGIGEGDRVIVY
                     GGRHLVASARVWWAFRVFGYDRVAVLDGGFPRWRDEGLPLESGESTPAPRRFLARMRH
                     ELVADLDRVRQILEHRGEIVVDARSHGRFVGTEPEPRPGVRSGHIPGSLNLPSDRLFG
                     PEGLLLPPEGLRRAFEAAGLDLDRPAVTACGSGVTAAVLAFGLYVLGREHAAVYDGSW
                     TEWGGRTDTPVER"
     gene            1630..>1846
                     /locus_tag="VEW91_09230"
     CDS             1630..>1846
                     /locus_tag="VEW91_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91800.1"
                     /translation="MVKKQLITGPAYTKGLGGRVWSPGIKVGPWLFLSGVTAVDYKTM
                     TTVGATGSPAAFTSPKLDPEAQWRQVLS"
BASE COUNT          266 a          638 c          639 g          303 t
ORIGIN      
        1 ccccacgatt ggccccgcct ccccccgaat ctcccggtgc cccaggacga cggggtctgt
       61 gaccacctgc ccgggaggcg cctgccggcg atctcgctgc cgtccacagc aggccgcctc
      121 gtcgagctgg cggccgtgcc cgggatcgcg gtcgtctact gctacccccg caccggccgg
      181 ccggatattc cgagcccgaa ggggtgggat gcgattccgg gggcgagggg atgcaccccg
      241 caggcgtgcg ccttccggga tcatcacggg gagttcctgg cgctcggggc gcgggtgttc
      301 ggcctcagca cccaaacgac cgaggaccag cgtgaggcga ccgagcggct ccacctgccc
      361 atggagttgt tgagcgacgc cggcctcgcg tttgcgcacg ccctgcaact tcccaccttt
      421 gaggtcgagg gaatggtgtt gatcaagcgg ctcacgctgg tcgcgcgcaa cgggcacatc
      481 accaaggtgt tctatccgat cttcccgccc gacgccaacg cgcaggaagt gctcacgtgg
      541 ctgcgggcac tcccacgatg agcgcgtcac ccctcgaccc cgcggtcagc ccggcttggg
      601 tggccgcgca ccttgccgag cccgatgtcc gggtcgtcga tgccacatgg tacgtgccgt
      661 ggctccatcg cgacgcccaa agggagttcc gcgaggccca cattccgggc gccgcctact
      721 tcgatatcga tgcgatcgca gaccggcgca cgaccctccc ccacatgctc ccggatgccg
      781 agtctttcgc ccagtctgtc ggggctctcg gcattggcga gggcgatcgc gtcatcgtct
      841 acggtggccg gcacctcgtc gcctccgcgc gggtctggtg ggccttccgg gttttcggct
      901 acgaccgggt ggcagtgctg gatggcggtt tccctcgctg gcgagacgag ggcctcccgc
      961 tcgagtcagg tgaatccacg cccgctccgc gccgcttcct ggcccgcatg cgccacgagc
     1021 tcgtggccga ccttgaccgc gtccgtcaga tcctcgagca ccggggcgag atcgtggtcg
     1081 atgcgcgctc gcatggtcgc ttcgtcggga ccgaaccaga gccgcgtccg ggggtgaggt
     1141 cgggccacat ccccggaagc ctcaacctcc cctctgatcg cctgttcgga cccgagggct
     1201 tgctgctccc cccggaaggc ctgcggcgcg cgttcgaggc ggctgggctg gatcttgacc
     1261 gtccggcagt cacggcgtgt ggatccgggg tcaccgccgc agtgctggcc ttcggcctgt
     1321 atgtcctggg acgggagcac gcggccgtct acgacggctc gtggaccgag tggggtggcc
     1381 gcacagacac gccggtcgag cgctagccag ggtggtcgcc ccgtgggaca tcctcggggg
     1441 aatggtctgg cggccggctg gtagcgccgg atcggggcgc cgcggtgtca gaggtcgtcc
     1501 tgatctgcac gtacaccgct cttgagtgcg aggagtctca gagggaaccg tggtcgccag
     1561 agccgaagtt acacaaggtt acggcgaatc gcgctccgca cgtgctcaca tcgagaaagg
     1621 aggtcaggga tggtcaagaa gcaactcatc acgggccccg cctacaccaa ggggctgggc
     1681 ggaagggtct ggagcccagg gatcaaggtg gggccgtggc tctttctctc cggggtcacc
     1741 gcggtcgact acaagaccat gacgacggtc ggcgccacgg gcagcccggc ggccttcacg
     1801 tccccgaagc tcgatcccga ggcgcagtgg cggcaggtgc tgagca
//
LOCUS       DASQGH010000470         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50166, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000470 DASQGH010000000
VERSION     DASQGH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50166"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VEW91_09235"
     CDS             <1..652
                     /locus_tag="VEW91_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91801.1"
                     /translation="TRSGDRLRLRSGAEPPVDLPAGPGGIEALTASGLRIRPKAITLT
                     MFARLCLGDLFVHGVGGGRYDRVTDALIAEVFGLTPPPYVVATATLCLPLLGTGDGAD
                     DGRALERRLMDLQHNPERYLEAPSPPQRGLIDEKWGLIRTVEGMRPGPERRAATHRIR
                     EVNRLLAQGLADEIDRTRARLAALDRSSGSQAAASFREYPFFLFDPAQVGALVAPP"
     gene            649..1356
                     /locus_tag="VEW91_09240"
     CDS             649..1356
                     /locus_tag="VEW91_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91802.1"
                     /translation="MDAVSAEDLWRSHGRQRVLRGASLTAPPGGCVAILGPNGSGKST
                     LLGILAAALRPGRGQVRIYGEDPFTIPGVRGRIGLVGHEPMLYGGLSVTENLGLLATL
                     YGLPDGRARAGRVCTLLGIDHPLIPVRRLSRGVQQRAALARALVHGPTVLLLDEPFTG
                     LDPGGVDDLRQILRGFCRDGGAIVLTTHSPAEAISVADQAAVLARGRITPPRPLEGVT
                     VETLRAWYASAAEGAIP"
     gene            1353..>1956
                     /locus_tag="VEW91_09245"
     CDS             1353..>1956
                     /locus_tag="VEW91_09245"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HYM91803.1"
                     /translation="MRVLAALIWKDLVIEARAREIVTAMSLVAFLALVILSLALGPSP
                     QLAAAAAPAAVWVTVSFAATLGLARAHALEQEGQALRGLLMTPVSRPVLYLGKCGTNL
                     VMVLLLQLVVVGATAVLLTGALGPHPARLALPLALGAVGFTAAGTLLGAMAAATRLRE
                     VLLPILLLPVVVPVIILSLAGIDAVIEDRAVSELFQTLNLL"
BASE COUNT          224 a          732 c          706 g          294 t
ORIGIN      
        1 cacgcgaagc ggggaccgcc tccgcctgcg ctccggcgcg gagccgccgg tggatcttcc
       61 ggccggcccc ggcgggatcg aggccctgac cgcatccggc ctccgcattc gccccaaagc
      121 catcacgctg acgatgtttg cgcggctgtg cctgggggat ctcttcgtgc acggcgtggg
      181 cggaggccgc tacgaccgcg tgaccgatgc gctgatcgcc gaggtgtttg gcctgacgcc
      241 ccccccgtat gtggtcgcca cggcgaccct ctgcctcccg ctcctgggga ccggggacgg
      301 ggccgacgac gggcgcgcac tcgagcggcg cctgatggat ctgcagcaca atccggagcg
      361 gtacctcgag gctccctccc ctccgcagcg cggcctgatc gatgagaagt ggggcctcat
      421 ccgcacggtc gaggggatgc ggcccgggcc ggagcgccgc gccgccacgc atcggatccg
      481 cgaggtcaac cgcctgctcg cgcaggggct ggcggacgag atcgaccgca cccgggcgag
      541 gctcgccgcc ctggatcggt cctcggggag ccaagccgcc gcgtcgttcc gggagtatcc
      601 gttcttcctg ttcgacccgg cccaggtcgg cgcgttggtg gcgcccccat agacgcggtc
      661 tcggcggaag acctctggcg cagccatggg cgccagcgcg tccttcgcgg ggcgagcctc
      721 accgcccccc cgggcgggtg tgtggcgatc ctcggcccca acggatcggg gaagtcgacg
      781 ctgctgggga tcctcgccgc ggcgctgcgc cccggccgag gccaggtgcg catttacggg
      841 gaagatccct ttacgatccc gggcgtccgg ggcaggatcg ggctggtcgg ccacgagccg
      901 atgctgtacg gagggctcag cgtgaccgag aatctgggcc tcctcgccac gctgtacggc
      961 ctccccgacg gccgcgcgcg ggcggggcgc gtctgtaccc tcctggggat cgaccacccg
     1021 ctcatcccgg tccgccgcct gtcgcgcggg gtgcagcagc gcgccgcgct cgcccgcgcc
     1081 ctcgtgcacg gccccacggt cctgctgctg gatgagccgt tcaccggcct cgacccgggg
     1141 ggcgtcgacg acctgcgcca gatcctccgc gggttctgcc gggacggcgg agcgatcgtc
     1201 ctgactaccc acagcccggc cgaagcgatc agcgtcgccg accaggccgc cgtgctcgcc
     1261 cggggccgca tcaccccccc gcgccccctg gagggggtga ccgtcgagac gctgcgggcc
     1321 tggtacgcca gcgccgccga gggggcaatc ccgtgagggt gctcgccgcc ctcatctgga
     1381 aggatctcgt catcgaggct cgggcccggg agatcgtgac cgcgatgagc ctcgtggcgt
     1441 tcctcgccct ggtgatcctc agcctcgcgc tggggccatc cccgcagctg gcggccgccg
     1501 ccgcgccggc cgccgtgtgg gtgacggtct cgtttgccgc gaccctcggg ctcgcccgcg
     1561 cgcacgccct cgagcaggaa ggccaggccc tgcggggact cctgatgacc ccggtcagcc
     1621 gcccggtgct ctacctcggc aagtgcggca ccaacctggt gatggtgctg ctgctacagc
     1681 tcgtggtggt cggcgcgacc gccgtgctgc tcaccggggc cctgggtccg cacccggcgc
     1741 gtctcgccct gccgctcgcc ctgggcgccg tcggcttcac cgcggcaggg acgttgctcg
     1801 gggcgatggc ggccgcgacg cggttgcggg aagtgctgct gccgatcctg ctcctgccgg
     1861 tggtggtgcc ggtgatcatc ctgtcgctcg ccggcatcga tgcggtgatc gaggaccgcg
     1921 cggtgtcgga actcttccaa acgttgaacc tgctgg
//
LOCUS       DASQGH010000471         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_38972, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000471 DASQGH010000000
VERSION     DASQGH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38972"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1135)
                     /locus_tag="VEW91_09250"
     CDS             complement(98..1135)
                     /locus_tag="VEW91_09250"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HYM91804.1"
                     /translation="MARIGLLTGGGDCPGLNAVIRAVVRRGLAGEGHSFLGFLHGWAG
                     VVEDHALELTLQSTAGILPRGGTILGTSRTNPYAEDRDGTAAVMHTLQSRGVDVLIPV
                     GGEDTLGVAQRLHEGGVPIVGVPKTIDNDLGGTDVTFGFQTAVQIVTDAIDRLHTTAE
                     SHNRVMVVEVMGRHAGWIATHAGIAGGADAILVPERPFDIDQVVAHLRRRHARGRSFS
                     IVVVAEGAAPREGTLQTVAGTETDAFGHPRLGGIGMALEGEIERRTGFETRVTILGHV
                     QRGGTPVAFDRVLATRFGVAAMDAAAGGRFGTMVGLRGTAIVEVPLADALREPKLLDP
                     GLYETAELFFG"
     gene            complement(1208..>1604)
                     /locus_tag="VEW91_09255"
     CDS             complement(1208..>1604)
                     /locus_tag="VEW91_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYM91805.1"
                     /translation="SILRKHGRLTPAEFETIKTHARTGYEMLAGSTSEVVRLGATIAH
                     THHERWDGGGYPRGLAGEEIPSEGRIAAVADVFDALTTDRVYRPAFPVRSAIDMMRAE
                     RAAHFDPEMLDAFMAALPDAEAVRRTYSD"
BASE COUNT          202 a          601 c          571 g          230 t
ORIGIN      
        1 cgcagaagca gctggccggg accgggttgg gggatgggac gctccaccgc ccgcaacggc
       61 tggccgacgg cctcgaagac catcgcccgc atggcgctca cccgaagaac agctcggcgg
      121 tctcgtacag cccgggatcc agcagcttgg gctcccgcag cgcgtccgcg agcgggacct
      181 cgacgatcgc ggtcccgcgc agccccacca tcgttccgaa gcgcccgccc gccgcggcgt
      241 ccatcgccgc cacgccgaag cgggtggcca ggacgcggtc gaaggccacc ggcgtgccgc
      301 cgcgctggac gtggccgagg atcgtcacgc gcgtctcgaa gcccgtccgc cgctcgatct
      361 cgccctcgag cgccatcccg atgccgccga gccgcgggtg tccgaaggcg tcggtctcgg
      421 tccccgccac cgtctgcagg gtcccctctc gcggcgcggc tccctccgcc accacgacga
      481 tcgagaagct gcggccgcgc gcgtggcggc gacggaggtg ggcgacgacc tgatcgatgt
      541 cgaacggccg ctcggggacg aggatcgcgt cggctccgcc ggcgatcccc gcgtgcgtgg
      601 cgatccagcc ggcatggcgc cccatgacct cgacgaccat cacccggttg tgcgactccg
      661 cggtcgtgtg caggcggtcg atcgcgtcgg tgacgatctg caccgccgtc tggaacccga
      721 acgtcacgtc ggtgccgccg aggtcgttgt cgatcgtctt gggcacgccg acgatcggca
      781 cgccgccctc gtgcaggcgc tgggcgacgc cgagggtgtc ctcgccgccg accggtatca
      841 gcacgtccac gccgcgactc tgcagcgtgt gcatgaccgc tgccgtgccg tcgcggtcct
      901 cggcgtaggg gttcgtgcgc gaggtgccga ggatggtgcc gccgcgcggg aggatgccgg
      961 cggtgctctg gagcgtcagc tcgagcgcat ggtcctcgac caccccggcc cagccatgca
     1021 ggaacccgag gaagctgtgc ccctcgccgg cgaggccccg gcgcacgacg gcccggatga
     1081 cggcgttcaa ccccgggcag tcgccgccgc cggtgagcag tccgatcctc gccattcccg
     1141 ttgacggtac cgcctcccgc ggaggattgc ccgcggcccg tccgcgtgcc tcccgctcgc
     1201 ctctccctca gtcgctgtag gtccgtcgga ccgcttcggc gtccggcagc gcggccatga
     1261 aggcgtcgag catctcggga tcgaagtgcg cggcccgttc cgctcgcatc atgtcgatcg
     1321 cagagcggac ggggaacgcg gggcggtaga cgcggtcggt ggtgagcgcg tcgaagacgt
     1381 ccgccaccgc ggctattcgc ccctcgctcg ggatctcctc gcccgcgagc ccccgcgggt
     1441 agccgccgcc gtcccagcgc tcgtgatggg tgtgggcgat cgtggcgccc agccgcacga
     1501 cctccgaggt ggacccggcg agcatctcgt agccggtccg ggcgtgggtc ttgatcgtct
     1561 cgaactccgc cggggtgagc ctgccgtgct tgcgcaggat gctg
//
LOCUS       DASQGH010000472         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4960, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000472 DASQGH010000000
VERSION     DASQGH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4960"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1062)
                     /locus_tag="VEW91_09260"
     CDS             complement(31..1062)
                     /locus_tag="VEW91_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM91806.1"
                     /translation="MEGTSAIPTRGEVTPKKTAATAMSPPTRRCAERSMTPCRPRGAS
                     CTRARARAGREEAGQIEVESSAHGAYCPPGACRRTPINVDLSGRVAVVSGASAGLGRA
                     IAQGLAEAGADTVVGSRDPEAIRRAATEIAASTGRQAVGVSADVSRPEGVDALIGTAL
                     QRFGKINILVANAGGPPPGSALTLTDAQWEQAFHQNLMSAVWLIRGAIPSMKAQGGGR
                     IITVITSGVKVPIPSLVLSNVFRSGVVALVKTLSLELAPHKILLNNLAPGRIRTRRTQ
                     EIDEATARASNRTVEQVEREMTAAIPAGRYGDPREFATMAVFLASDHASYLTGTTICI
                     DGGATRAMQ"
     gene            959..2227
                     /locus_tag="VEW91_09265"
     CDS             959..2227
                     /locus_tag="VEW91_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91807.1"
                     /translation="MERSAHRRVGGLIAVAAVFFGVTSPRVGIAEVPSIPLPPVRVEV
                     TGNPAPVETQRLAILTAARALVPEARSGQVSLTETTPPLQPLSVDSEMALRAIVQVTA
                     GGAEPVTRAVPVAIHNEAMPWTDAQALLISNSPETLAFSKVLLNASVASGETVRLLYH
                     HANGSPTRRMTIGVNLSNPARDPIKLWVIGAAGRAGAGELALGHAAARTFLDQYWHHA
                     GFIVQIPANTTLPLFLHDLAPGGIASGLTQFTLLAGDRLNVQVFARLEGEIDPPTMSY
                     APNFDRVHQRGVFDRPQLVQPQAYTVGDPSVSMNVGDEQDALRESQTGVGLDGNYGAV
                     YTFPIEVSNPRTLPSLLGVVMHALSGEASGTVLVDGRIIDIPRLRDGERRLVTTIHLA
                     PGEHRPLVVSTMPESGGYYPVRLTLGAEYK"
     gene            2307..3464
                     /gene="proB"
                     /locus_tag="VEW91_09270"
     CDS             2307..3464
                     /gene="proB"
                     /locus_tag="VEW91_09270"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYM91808.1"
                     /translation="MCPGAHPRSFEEDASHRSFRRIVLKVGTSTLTGGSAVVDPHRLA
                     MLADDVAAAIRRGREVLVVSSGAIVTGTGLLGLRRPAHDLAQKQALAAVGQPLLMQHY
                     AAAFDALGLRVGQVLLTQRDFAIRHRYVNARRTLEALLALGVIPIINENDTIATDEIQ
                     IGDNDTLSALVASLVGADLLVILSDVEGLMTADPRRHRDAHLIPVVPRIDATVLRAAR
                     RTPSAQGVGGMATKISAARTATLSGVVTVITSGVPPNPLARLLEGERHGTMFLPARRP
                     ASSRRRWLALGLPARGALVIDDGARAALERGKSLLAAGIVDVQGTFEPGEAVVIRDTQ
                     GAEIARGVSSYPSGQVAQIMGIRSAEIARVLGVRTPREVVHRDNLVLTSRK"
     gene            3475..4728
                     /locus_tag="VEW91_09275"
     CDS             3475..4728
                     /locus_tag="VEW91_09275"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HYM91809.1"
                     /translation="MSTTYPEVDLRAREARSAAAVLHHAPDALRRRALRAMAEAIQAG
                     RGAIQEANRRDLTALVDRPPAFRDRLTLTDTRIDGLSQALDAIAALPDPIGQVVESRL
                     QPNGMQVTRIRVPLGVIGLIYESRPNVTVDAAGLCLRAGNAVILRGGAESVHSDDALV
                     RAMHRGLREAGVPPEAISMLEHRDYQAIRELVGPRGLVDLVIPRGGEALIRFVTEHAR
                     VPVLKHERGVTHIFVDRAADPEMARRIIVNAKVNRPGTCNALEAVLVDAEIAGRLLPP
                     LVAALHAQGVEVRGCPETRRHVPGVTPATDADWGAEYLDLILAVRVVDGFDQAVAHIR
                     TYGTGLADGIVTEDRGRAERFVTEVDSAAVLVNASTRLVDGGEFGRGAEIGISTSRLH
                     ARGPMGLEELTTTKWIVRGTGQIRE"
BASE COUNT          725 a         1770 c         1759 g          697 t
ORIGIN      
        1 aggggcggtg aggacggtcg cgggccatcg ttactgcatc gcgcgggtcg ccccgccgtc
       61 gatgcagatc gtggtgccgg tgaggtagct cgcgtggtcg gaggccagga acaccgccat
      121 ggtggcgaac tcccgcggat cgccgtaccg ccccgccgga atcgccgcgg tcatctcgcg
      181 ctccacctgc tcgacggtgc ggttgctcgc ccgcgccgtc gcctcgtcga tctcctgggt
      241 gcgccgcgtg cggatgcggc ccggcgcgag gttgttgagc aggatcttgt gcggcgccag
      301 ttcgagcgag agcgtcttga ccaacgcgac cacccccgac cgaaagacgt tgctcaggac
      361 gaggctcggg atcgggacct tgaccccgga ggtgatcacc gtgatgatcc gccctccccc
      421 ctgggccttc atcgatggga tggctccgcg gatcagccac accgcgctca tcaggttctg
      481 gtggaacgcc tgctcccact gcgcatcggt cagcgtgagc gccgaccccg gcggcggccc
      541 cccggcattg gccacgagga tgttgatctt cccaaagcgc tggagggcgg tgccgatgag
      601 cgcgtccacc ccctccggcc gcgagacatc cgccgacacc cctaccgcct gccggccggt
      661 gctggcggcg atctccgtcg ccgcgcgccg gatcgcctcc gggtctcggc tgccgaccac
      721 ggtgtcggcg ccggcctccg cgagcccctg ggcgatcgcg cgtcccaacc ccgcgctcgc
      781 gccggagacg accgcgaccc ggcccgacag atcgacgttt atcggtgtcc tccggcacgc
      841 gccgggtggg caataagcac catgggcact ggattcgacc tcgatctgcc cggcttcctc
      901 tctaccggcc ctcgcccgcg cacgtgtaca actcgcaccg cgaggccgac aaggagtcat
      961 ggagcgctca gcgcaccggc gagtgggtgg actcatcgca gtggcggcgg tcttcttcgg
     1021 ggtgacctcg ccccgggtcg ggatcgccga ggttccttcc atcccgctcc ccccggttcg
     1081 cgtcgaggtc accgggaacc cggcccccgt cgaaacccag cgcctcgcca ttctcacagc
     1141 ggcccgggct ctggtgcccg aggcgcgcag cggccaggtg tcgctcaccg agaccacgcc
     1201 gcccctccaa ccgctgtcgg tggattccga gatggcgctg cgcgcgatcg tacaggtcac
     1261 cgccggaggc gccgagccgg tgacccgagc cgtcccggtc gcgattcaca acgaggcgat
     1321 gccgtggacg gacgcgcagg cgctgctcat cagcaacagc cccgagaccc tggcgtttag
     1381 caaggtgttg ctcaacgcgt cggtcgcctc cggcgagacc gtgcggttgc tctaccacca
     1441 cgcgaacggg tcgccgaccc ggcggatgac gatcggggta aacctcagca acccggcgcg
     1501 ggacccgatc aagctttggg tcatcggcgc ggcgggacgg gccggcgcgg gcgaactggc
     1561 gctgggccac gccgccgccc gcacgtttct cgatcagtac tggcaccacg ccgggttcat
     1621 cgtgcagatc ccggccaaca ccacgctgcc gttgttcctc cacgacctcg cgcccggggg
     1681 gatcgccagt ggcctcacgc agttcacgct cctcgccggg gaccggctca acgtccaggt
     1741 gttcgcccgg ctggagggcg agatcgaccc accgacgatg agctacgcgc ccaacttcga
     1801 tcgggtccac cagcgggggg tgtttgaccg tccccagttg gttcagccgc aggcctacac
     1861 cgtcggcgac cccagcgtgt cgatgaacgt gggggacgaa caggacgcgc tccgcgaaag
     1921 ccagacgggg gtcggcctcg acggcaacta cggggccgtc tataccttcc cgatcgaggt
     1981 gagcaatccc aggacgttgc cgtcgctcct gggggtggtg atgcacgcgc tgtcgggcga
     2041 ggcgagcggc acggtgctcg tggacggccg gatcatcgac atcccgcgcc tccgcgacgg
     2101 cgagcggcgc ctcgtgacca cgatccatct cgcgcccggg gagcatcgcc cgctcgtggt
     2161 ctcgacgatg cccgagagcg gcgggtacta cccggtccgt ctcacgctcg gggccgagta
     2221 caagtaacgg ccggccgggc ccatttcggc ccggaccacc cggcaggagg aagccccgcg
     2281 gcgcggccag aaatccggtc accctgatgt gtcccggcgc ccacccccgg tcgttcgagg
     2341 aggacgcgag ccaccggagc ttccggcgga ttgtgctcaa ggtggggacg agcaccctca
     2401 ccggcgggag cgcggtggtc gacccccacc gcctcgccat gctcgccgac gacgtggccg
     2461 cggcgatccg gcgcgggcgg gaggtcctcg tcgtctcctc gggcgcgatc gtgaccggta
     2521 ccggcctgct cggcctccgc cgcccggccc acgacctcgc ccagaagcag gcgctggccg
     2581 cggtggggca accgctcctg atgcagcact acgcggcggc gttcgacgcg ctcgggctgc
     2641 gggtggggca ggtcctcctg acccagcggg acttcgcgat ccggcaccgg tacgtcaacg
     2701 cccggcggac gctcgaggcg ctgctcgccc tcggggtgat ccccatcatc aacgagaacg
     2761 acaccatcgc caccgacgag atccagatcg gcgacaacga cacgctgtcc gcgctcgtcg
     2821 cgagcctggt cggcgccgat ctgctggtca tcctctccga tgtcgagggg ctgatgaccg
     2881 ccgacccgcg ccgccaccgg gatgcgcacc tcatcccggt ggtgccgcgc atcgacgcga
     2941 ccgtgctgcg cgcggcccgg cggacgccga gcgcccaggg ggtcggcggg atggcgacga
     3001 agatcagcgc ggcgcggacc gctaccctct ccggcgtggt cacggtgatc acgagcggcg
     3061 tccctcccaa tccgctcgcg cggctcctcg aaggcgagcg acacgggacc atgttcctcc
     3121 ccgcgcgccg ccccgcgtcg agccggcggc ggtggctggc gctcgggctg cccgcgcggg
     3181 gtgcgctcgt catcgacgac ggcgcgcgcg cggccctcga gcgcggcaag agcctgctgg
     3241 ccgcgggcat cgtcgacgtg caggggacgt tcgaaccggg cgaggccgtg gtgatccggg
     3301 acacccaggg ggcagagatc gcccggggcg tgtccagcta cccgagcggt caggtggcgc
     3361 agatcatggg catccggtcg gcggagatcg cccgcgtgtt gggggtccgg acgccccggg
     3421 aggtcgtcca ccgggacaac ctcgtcctga cctcccggaa gtgaggagga gaccgtgagc
     3481 accacatacc ccgaggtgga cctgagggcg cgggaggcgc ggagcgcggc ggccgtgctg
     3541 caccacgctc ccgacgcgct ccgccggcgc gcgctccggg cgatggcgga ggcgattcag
     3601 gccgggcgcg gtgcgatcca ggaggccaac cgccgcgacc tcaccgccct cgtggaccgc
     3661 ccgcccgcgt tccgcgaccg gctgacgctg accgacaccc gaatcgacgg attgtcccag
     3721 gccctcgacg ccatcgccgc cctgccggat cccatcggcc aggtggtgga gtcacgcctc
     3781 caaccgaacg ggatgcaggt cacgcggatc cgggtcccgc tcggggtgat cggcctgatc
     3841 tacgagtcgc ggccgaacgt gacggtcgat gcggccgggc tgtgcctgcg cgccggcaac
     3901 gcggtaattc tgagaggcgg cgcggagtcg gtgcactccg atgacgcgct ggtgcgcgcc
     3961 atgcaccgcg ggctgcgcga ggcgggggtg cctccggagg cgatctcgat gctcgagcac
     4021 cgcgactacc aggcgatccg cgagctggtg ggaccgcggg gcctggtgga cctggtgatt
     4081 ccccggggcg gggaagcgct gatccggttc gtcaccgagc acgcccgcgt cccggtgctc
     4141 aagcatgaga ggggcgtgac ccacatcttc gtcgaccgag ccgccgatcc ggagatggcc
     4201 cggcgcatca tcgtcaacgc caaggtgaac cggccgggca cctgcaacgc gctcgaggcg
     4261 gtgctggtgg acgccgagat cgccggtcgg ctgctccccc cgctcgtagc ggcgctccac
     4321 gcccaagggg tcgaggtgcg gggctgcccc gagacccgcc ggcacgtccc gggggtcacc
     4381 ccggcgacgg acgccgattg gggggcggag tatctggacc tgatcctggc ggtgcgggtg
     4441 gtcgacggat tcgatcaggc ggtggcgcac atccggacct acggcacggg cctcgccgat
     4501 gggatcgtca ccgaggaccg ggggcgggcc gagcggttcg tgacggaggt cgacagcgcc
     4561 gccgtgctgg tcaacgcgtc gacccgcctg gtggacggcg gggagttcgg gcgcggcgcc
     4621 gagatcggca tcagcaccag ccggctgcac gcccgcgggc cgatggggct cgaggagttg
     4681 acgacgacga agtggatcgt aagggggacc ggccagatcc gcgagtgagg gagccgggat
     4741 ctcaggagaa gagctccgct tcacccccgg gccggagcca gagttcagga aactgatagc
     4801 ccgataagaa ctccatcaac cctggagggg ctcaagtgtc acccgtgcga atattaccgc
     4861 agattttctc tcccaacgct ttgcccgaga agatggagta tctcgacgcg atcgaggcat
     4921 cagcttcgga agagcatctg cggtaggtcc g
//
LOCUS       DASQGH010000473         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_4962, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000473 DASQGH010000000
VERSION     DASQGH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4962"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2411)
                     /locus_tag="VEW91_09280"
     CDS             complement(<1..2411)
                     /locus_tag="VEW91_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYM91810.1"
                     /translation="MTGDGGMRIIGRPWAKVDGPAKTTGETRYADDLFLPRMLYGKFL
                     RSHLPHARIVRLDVTRAQALPGVHAVLTGRDLPTKFGIMPSSQDEEALCLDKVRYVGD
                     PIAAVAAVDEETAERACEAIEVEYEPLRAVMSIEDGLRDAEVRVQEYGDGPTPNIHKN
                     ISLEFGDLDRGFAEADHVREDAFFFQGNTHLPMEQHSAVAQYESNGRLTLWTSTQTPH
                     YVHRALSGVLGLPMGRIRVIATPVGGGFGGKSDPFSHEICAAKLAMQTGRPVKITLTR
                     EEVFYAHRGRHPVLMWIRTGVKRDGRITACHFRTFLDGGGYGSYGIASTYYTGALQTV
                     TYRIPAYKFEGTRVWTNKPPCGPKRGHGTPQPRYGIEIQMDKLAEDLGLDPLTIRLAN
                     ATEPFTRTVNHLRITSNGLRECIERVAAASGFREKHRRLPPGRGVGFAVSAYLSGAGL
                     PIYWNDMPHSEVQIRVDRGGGVIVSCGAIDIGQGSDSVLSGIVAEILGLDPREISLVT
                     ADTDLTPIDLGSYSSRVTFMAGNAAVQAAQKMRDLLVAAASEHLEVDPDGLRVGDHRI
                     YDGAHPSRGVTFPEAAQLAEAMFGTLTTVGSYRPPKLAGPYKGSGVGPSPAYSFSAAV
                     VEVRADRGTGDVVVERVWIAHDIGRAINETLVIGQIEGSVYMALGEALMEEQIFRKGL
                     HKIPSMLEYKSPTFLEMPPVETILVNTDDPEGPFGAKEAGQGPLLPVIPALANAVYDA
                     VGVRVDEIPISPDKVLAALEQKARGGEGRVGPRAVPPFRFRDPIKVEPPPGWEAPWRL
                     AQPIAG"
     gene            complement(2408..2962)
                     /locus_tag="VEW91_09285"
     CDS             complement(2408..2962)
                     /locus_tag="VEW91_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYM91811.1"
                     /translation="MKVTLTLRVNGEDRDVVVPVHKTLLEVLREDLGLTGTKHGCELG
                     ECGTCTVLVDGTPVLSCLTLPVELQGRAITTVEGLARGGRLHPLQEAFAELGAAQCGY
                     CTPGILVAAKSLLDRNPTPTRSDVAEWLAGNLCRCTGYLKILEAVELAAARMKGVGPV
                     APAAASPAAAPAGGARPSQEGAGR"
     gene            complement(2959..3762)
                     /locus_tag="VEW91_09290"
     CDS             complement(2959..3762)
                     /locus_tag="VEW91_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYM91812.1"
                     /translation="MPRVVASRGRSASVPAVDRAARLLLALGDGQKDATLTHLAGTLR
                     IHKSTAHNILATLIQHRFVDRDPATRTYRLGSALAALGGVVAGRQDLVALARPHLVRL
                     QRLSGETSTLHLRDGTGSIILASEESPHQLKVTAPPGHRLPPFAGAVAKVLLAFGEPA
                     AGPLPARLPVYTRRTITAPGRYLEELRWVRRAGVAYDEMEYLPGVRAVSAPIFGGPND
                     QGRAVGALSIVGVGARISPQDLRRLAGPLRNAARTLSAALQPARGGRPS"
BASE COUNT          547 a         1350 c         1308 g          591 t
ORIGIN      
        1 cccgcgatcg gttgggcgag gcgccagggc gcctcccagc ccggcggcgg ctcgaccttg
       61 atgggatcgc gaaaccggaa cggaggcacg gcgcgggggc ccacccggcc ctcacccccc
      121 cgggccttct gctccagcgc cgcgaggacc ttatcgggcg agatcgggat ctcgtcgacg
      181 cggacgccaa cagcgtcgta caccgcgttg gccagcgcgg ggatgaccgg aagcagcggt
      241 ccctgccccg cctccttggc gccgaacggt ccctcgggat cgtccgtgtt gaccaggatc
      301 gtctccaccg gcggcatctc gagaaacgtc gggctcttgt actcgagcat cgagggaatc
      361 ttgtgcagcc ccttccgaaa gatctgctcc tccatcagcg cctcgccgag cgccatgtag
      421 acgcttccct cgatctgccc gatgaccagg gtctcgttga tcgcgcgtcc gatatcgtgg
      481 gcgatccaga cgcgctcgac cacgacatcg cccgtccccc gatccgcccg gacctcgacc
      541 accgcggccg agaacgagta cgccggcgac ggcccaacgc cggaaccttt gtacgggccg
      601 gccaacttcg gcggccggta ggacccgacg gtggtcagcg tcccaaacat cgcctcggca
      661 agttgggccg cctcgggaaa cgtcaccccg cgcgaagggt gggctccatc gtagatgcgg
      721 tggtcgccca cgcggaggcc atcggggtca acctccaggt gctcggaggc ggccgccaca
      781 agcaggtccc gcatcttttg cgccgcttgg acggcggcgt tgcccgccat gaacgtcacc
      841 cggctgctgt acgacccgag atcgatcggg gtgagatccg tgtcggcggt caccaggctg
      901 atctcgcggg gatcgagccc caggatctcg gcgacgatcc cgctcaacac cgagtcgctt
      961 ccctggccga tgtcgatcgc gccgcaggac acgatgacgc cgcccccgcg gtcgacgcgg
     1021 atctgcacct cgctgtgggg catgtcgttc cagtagatgg gcaggcccgc gccgctcagg
     1081 taggcgctga ccgcaaaccc cacgccgcgg cccggcggca ggcgacggtg cttctcccgg
     1141 aacccgctcg ccgccgcgac ccgctcgatg cactcccgga ggccgttgct ggtgatccgg
     1201 aggtggttca cggtcctggt gaagggctcg gtggcgttcg ccagccggat cgtgaggggg
     1261 tcgagcccga gatcctcggc aagcttgtcc atttggatct cgatgccgta gcggggctgc
     1321 ggggtgccgt gcccgcgctt cggaccgcac ggcggcttgt tggtccacac ccgcgtcccc
     1381 tcaaacttgt aggcgggaat cctgtaggtg acggtctgca gcgcgccggt gtaataggtg
     1441 ctcgcgatcc cgtatgatcc atacccgccc ccgtcgagaa aggtcctgaa gtggcacgcg
     1501 gttatccggc cgtcacgctt gacgccggtc cggatccaca tcaggacggg gtgccggccg
     1561 cggtgggcgt agaagacctc ttcgcgggtg agggtgatct tgaccgggcg cccggtctgc
     1621 atcgcgagtt tggccgcgca gatctcgtgg ctgaacggat cgctctttcc gccaaaccct
     1681 ccgcccacgg gggtggcgat cacccggatc cggcccatcg gcaggccgag gaccccgctc
     1741 aacgcccggt ggacgtagtg cggggtctgg gtcgaggtcc agagggtcag ccgcccgttg
     1801 gactcgtact gggcgaccgc gctgtgctgc tccatcggca ggtgggtgtt gccctggaag
     1861 aagaacgcgt cctcccgcac gtggtccgcc tccgcgaacc cccggtcgag gtcaccgaac
     1921 tccagcgaga tgttcttgtg gatgttgggg gtcggtccgt ccccgtactc ctgcacccgg
     1981 acctcggcgt ccctgagccc gtcctcgatc gacatgaccg cccgcagcgg ctcgtattcc
     2041 acctcgatcg cctcgcaggc ccgctcggcc gtctcctcgt ccaccgcggc cacggccgcg
     2101 ataggatcgc cgacgtagcg caccttgtcg aggcacaacg cttcctcgtc ctggctgctc
     2161 ggcatgatcc cgaacttcgt ggggaggtcg cggccggtca gcacggcgtg cacgccgggg
     2221 agggcctgcg cccgggtcac gtccagccgg acgatccgcg cgtggggcag gtggctgcgg
     2281 aggaacttcc cgtacagcat tctcgggagg aacagatcgt ccgcgtagcg ggtctcgccc
     2341 gtcgtttttg ccggcccatc gaccttggcc cagggccggc cgatgatccg catccccccg
     2401 tcccccgtca tcgcccggcc ccttcctgag aggggcgcgc gccgccggcg ggggctgcgg
     2461 ccggcgatgc agcggccggc gccaccggcc cgacgccctt catccgcgcg gccgccagtt
     2521 ccacggcctc gaggatcttg aggtagccgg tgcaccggca gaggtttccg gcgagccact
     2581 cggccacatc gctccgggtg ggcgtgggat tgcgatcgag caacgacttg gccgcaacga
     2641 ggatccccgg cgtacagtag ccgcactgcg ccgccccgag ttccgcgaac gcctcctgga
     2701 gaggatggag ccgcccgccc cgcgcgagcc cctcgacggt cgtgatcgcc cgcccctgga
     2761 gctcgaccgg cagggtgaga cagctcagca ccggcgtgcc gtccacgagc acggtgcacg
     2821 tcccgcactc acccagttcg cacccatgct tggtcccggt gagtccgaga tcttcgcgca
     2881 gcacctcgag gagggtcttg tgcacgggca cgaccacgtc gcggtcctcc ccgttgacgc
     2941 gaagcgtcag ggtcaccttc acgagggcct cccgcctcgg gccggctgta gcgccgcgga
     3001 gagggtccgc gcggcgtttc ggagcggacc cgcgagccgg cgcaggtcct ggggggagat
     3061 gcgggcgccg acgccgacga tcgagagcgc ccccacagcg cggccctgat cgttcggtcc
     3121 cccgaagatg ggggctgaga cggcacggac gccgggaagg tattccatct catcatacgc
     3181 cacgccggcg cgcctgaccc accgcagttc ttcgaggtat cgcccgggag cggtgatcgt
     3241 gcgccgcgtg tacacgggga gccgggcagg cagcggcccg gccgcgggct ccccgaacgc
     3301 caggagcact ttggcgacgg ctcccgcaaa cggcggcagc cggtgccccg ggggagcggt
     3361 gaccttgagt tggtgcgggg actcctcgct ggcaaggatg atgctgcccg tcccatcgcg
     3421 cagatggagc gtcgatgtct cgcctgaaag ccgttggagg cggacgaggt gcgggcgggc
     3481 gagcgcgacg agatcctggc gcccggccac cacccccccc agcgccgcca gcgccgagcc
     3541 gagccggtac gtgcgggtcg ccggatcgcg gtcgacgaac cggtgctgga tcagcgtagc
     3601 gaggatgttg tgcgcggtgc tcttgtggat gcgcagggtg ccggcgaggt gggtgagggt
     3661 cgcatctttc tggccgtcgc ccaacgccag cagcagccga gcggcccggt cgaccgccgg
     3721 aaccgatgcg gagcgccccc gagacgccac aacccgcggc atgcgtttgt tctccttaga
     3781 gaactatgtt ccctct
//
LOCUS       DASQGH010000474         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30512, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000474 DASQGH010000000
VERSION     DASQGH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30512"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VEW91_09295"
     CDS             <1..528
                     /locus_tag="VEW91_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HYM91813.1"
                     /translation="EGPAPARAPRLAPGQDAIRGLYCGGSLAEEADLVLHRLLGDVPA
                     GTHTVVDLGADEYTVGRPHPMIDPRLRNQHIVDAARHPRVAVILLDVVLGYNAHPDPA
                     GALVPALEDARREAGRAGRQVAVVASVCGTPGDPQDLRRQEATLAAAGVFLAPSNAQA
                     ARLAARLARPPGGAV"
     gene            525..>1652
                     /locus_tag="VEW91_09300"
     CDS             525..>1652
                     /locus_tag="VEW91_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HYM91814.1"
                     /translation="MTRASARPESALFPDGIRALNVGLAGFAAPVRAHGAVCLHVQWR
                     PPAQADRELGLLLARLEDDPDDPVGALVASANARAVQRILEGRPALLDVQPAGKVVPG
                     MGARMLLHSGPPIEWARMCGPMRGAVIGAVLLEGWATTPGEAEAMAARGEIGFAPCHH
                     HQAVGPMAGIVSPSMPVCVVENTSHGTRAFATLNEGLGKVLRFGAYDADVIRRLRWFA
                     ETLGPALSRALQADGPVDLLSLSAQALQMGDECHNRNAAATSLFARTMAPALVRAVER
                     ETAAAILEFFRSNDHFYLNLSMAACKATLDPAHGIPGSTVVTAMARNGVEFGIRLSGA
                     QDAWFTAPSEVPDGLYFPGYGPEDANPDLGDSAITETCGLGG"
BASE COUNT          204 a          602 c          610 g          236 t
ORIGIN      
        1 gaggggccgg cgccggcccg agccccgcgg cttgcgccgg gacaggatgc gatccgcggg
       61 ctgtactgtg gcggctccct ggccgaggaa gcggacctcg tcctccaccg gctgctcggg
      121 gacgtccctg ccggcaccca cacggtggtt gatctcggcg ccgacgagta caccgtcggc
      181 cgcccccatc ccatgatcga tccgcgcctc cggaaccagc atatcgtgga tgcggcgcgg
      241 catccgcggg tggcggtgat cctcctcgac gtggtcctcg ggtataatgc gcacccggat
      301 cctgccggcg ccttggtccc cgcgctcgag gacgcccggc gggaggccgg gcgtgcgggg
      361 cgccaggtgg ccgtcgtggc cagcgtctgc ggcacgccgg gcgatcctca ggaccttcgc
      421 cggcaggagg caaccctggc ggcggccggg gtcttccttg cgccgagcaa cgcgcaggcc
      481 gcccggctcg cggcccggct cgcgcggccg cctggcggtg ccgtgtgacc cgggcctcgg
      541 cgcgtcccga gtcggcgctc ttccctgacg gcatccgggc cctgaacgtt ggcttggcgg
      601 ggttcgccgc gccggtccga gcccacggcg cggtctgcct gcacgtgcag tggcgtccgc
      661 cggcccaggc ggaccgcgag ctgggactcc tcctcgcccg cctggaggac gatccggacg
      721 acccggtggg cgctctggtg gccagcgcca acgcccgcgc ggtccagcgg atcctcgagg
      781 gccgccccgc gctgctcgac gtgcagccgg cggggaaggt cgtccccggg atgggcgcgc
      841 gcatgctgct gcacagcggc cctccgatcg agtgggcacg gatgtgcggg ccgatgcgcg
      901 gcgcggtgat cggggccgtc ctcctcgagg gctgggcgac cacgcccggc gaagcggaag
      961 cgatggccgc ccggggcgag atcgggtttg caccctgcca ccaccaccag gcggtcgggc
     1021 cgatggccgg gatcgtctcg ccctcgatgc cggtgtgcgt ggtggagaat acgagccacg
     1081 gtacccgcgc gtttgccaca ctgaacgagg ggctcgggaa ggtgctgcgg ttcggggcct
     1141 acgatgcgga tgtgatccgc cggttgcgct ggtttgcgga gacgctcggc cccgcgctca
     1201 gccgcgcgct gcaagcggac ggtccggtcg atctcctgag cctctccgcc caggccctgc
     1261 agatggggga tgagtgtcac aaccgcaacg ccgctgcgac gtcgctgttc gcccgcacga
     1321 tggcgcccgc cctcgtccgg gccgtcgagc gcgagaccgc cgcggcaatt ctcgagttct
     1381 tccggagcaa cgaccacttc tacctgaacc tctcgatggc cgcctgcaag gccaccctcg
     1441 accccgcgca cggcatcccc ggctcgaccg tggtcacggc aatggcgcgg aacggcgtcg
     1501 agtttgggat ccgtctaagc ggcgcgcagg acgcgtggtt caccgcgccg tccgaggttc
     1561 cggacggcct gtacttccca gggtacgggc ccgaggacgc caacccggat ctgggcgaca
     1621 gcgccatcac ggaaacatgc gggctgggcg gc
//
LOCUS       DASQGH010000475         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30514, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000475 DASQGH010000000
VERSION     DASQGH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30514"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VEW91_09305"
     CDS             complement(<1..293)
                     /locus_tag="VEW91_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91815.1"
                     /translation="MAALAADRFAAIRAAIEAAFPVQGVELRDGRPVFDVASGVDDKA
                     RFLRLRQDLDVLGVLPLLRRREGRTVILLAPKPPPTPVQWTLPLALFLATFLTT"
     gene            complement(393..1463)
                     /gene="ftsZ"
                     /locus_tag="VEW91_09310"
     CDS             complement(393..1463)
                     /gene="ftsZ"
                     /locus_tag="VEW91_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYM91816.1"
                     /translation="MAGTERDLRRYAAIKVVGVGGGGSNAVNRMISAGLRGVEFIAVN
                     TDAQALALSNADKKIHIGAKLTKGLGAGGDPEIGRQAAEEGREELTEALEGSDMVFVT
                     AGMGGGTGTGGAPIVAQAGRELGALTIGVVTRPFGFEGRRRALTAEEGIRTLRQRVNT
                     VITIPNDRLLQVVEKTATVVEAFRVADDVLRQGVQGIADLITVPGLINLDFADVRTVM
                     AEAGSALIGIGVADGEGRAVKAAQAAIASPLLETSMEGARGILMNITGGVDLTLLEVS
                     EAAQVVQKAADPDANIIFGAVIDDQLDTEVRITVIATGFEGARSELDEAAGSAMAGQG
                     DAGGTPRAEPDDLDIPAFLRKR"
     gene            complement(1653..>1882)
                     /locus_tag="VEW91_09315"
     CDS             complement(1653..>1882)
                     /locus_tag="VEW91_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91817.1"
                     /translation="NMFTERLGMVARVGVPEIPGSVADTVRSPAYATGVGLVLHAARQ
                     RKLGRAARSLNGNGTVYGRVRQWLREFALGT"
BASE COUNT          250 a          718 c          625 g          289 t
ORIGIN      
        1 gtggtcagga aggtggcgag aaagagcgcc aacggcaggg tccattggac cggggtgggg
       61 ggcggcttgg gggcgaggag gatgacggtc ctcccctccc ggcgcctgag gagcgggagc
      121 acgccgagga catcgaggtc ctggcgcagg cgcaggaacc gcgccttgtc gtcgacgccg
      181 gaggcgacgt cgaatacggg gcggccgtcc cggagctcaa ccccctggac cggaaatgcc
      241 gcctcgatcg ccgcccggat cgcggcaaac cgatccgcgg caagggctgc catggcttct
      301 agcctaccac cggagggaag tgcggaaaag aggcggcgcg gccgacggcg gcgtcacacc
      361 agcgccgccg tcatggcccg ggcctgacgg gtttagcgct tccgcaagaa ggccgggatg
      421 tccaggtcgt cgggctccgc ccggggggtg cccccggcgt cgccctgccc ggccatcgcc
      481 gacccggcgg cctcgtcgag ctccgaccgg gccccttcga acccggtggc gatcaccgtg
      541 atccgcacct cggtgtcgag ctggtcgtca atgaccgcac caaagatgat gttggcgtct
      601 ggatcggccg ccttctgcac cacctgcgcg gcctcgctca cctccagcag cgtgaggtct
      661 accccgccgg tgatgttcat gaggatcccc cgggcgccct ccatggacgt ctcgagcagc
      721 gggctcgcga tcgccgcctg ggccgctttg acggcacggc cctccccgtc cgccaccccg
      781 atcccgatga gcgccgaccc ggcctcggcc atcaccgtgc ggacatcggc aaaatcgagg
      841 ttgatgagcc cggggaccgt gatcaggtcc gcgatgccct gcacgccctg gcggagcacg
      901 tcgtcggcga cgcgaaacgc ttcgaccacg gtggcggttt tttccaccac ctggagcaac
      961 cggtcgttgg ggatcgtgat caccgtgttg acgcgctggc ggagggtgcg gatcccttcc
     1021 tcggccgtca gcgcccggcg gcgtccctcg aagccgaacg ggcgggtgac gaccccgatg
     1081 gtcaaggcgc ccagttcccg gccggcctgc gccacgatcg gcgcgccacc ggtccccgtc
     1141 cccccgccca tgccggcggt gacgaagacc atgtcggagc cttcgagcgc ctcggtcagc
     1201 tcttcccggc cctcctcggc cgcctggcgg ccgatctccg ggtccccgcc ggcgccgagg
     1261 cccttcgtca gcttggcccc gatgtggatc ttcttgtcgg cgttgctcag cgccaacgcc
     1321 tgcgcgtccg tgttgacggc gataaactcg acgccccgga ggcctgcgct gatcatgcgg
     1381 ttcaccgcgt tgctgccccc cccgccgacc ccgacgacct tgatcgccgc gtaccgccgg
     1441 aggtcccgct ccgttcccgc catcttcacc cctcccgccg gatctggagg agatccccgc
     1501 ttctcccccg cgcgcgttcc cgcatttgcc gactcggatc cccaggtgag cattcgctca
     1561 atggggtgcc gcgaccgtgg tgtcgtcacc atcctgcttc ggctcctctc gtccctgcgg
     1621 cccccccccg gggcggtgcg gccccggggc gcttatgtgc cgagggcgaa ctcccgcagc
     1681 cactggcgga cccgcccgta cacggttccg ttgccgttca ggctgcgcgc ggcgcgtccc
     1741 agcttccgct gccgcgcggc gtggagcacc agcccgacgc ccgtcgcgta cgccgggctg
     1801 cggaccgtgt ccgccacact cccggggatc tcgggcaccc cgacccgagc caccatcccc
     1861 aaccgctcag taaacatgtt gg
//
LOCUS       DASQGH010000476         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2003, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000476 DASQGH010000000
VERSION     DASQGH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VEW91_09320"
     CDS             complement(<1..1104)
                     /locus_tag="VEW91_09320"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HYM91818.1"
                     /translation="MAALRELPQLIDADRRDVLSVALDVDPTKPEHQTPNPAYRIWVH
                     QALARVLEAAPGAARRDIGAAAHRIQGRLTAGPVPGHGLVFLAAPDLWGEYALPDPVP
                     NRILYGRPDLLPLLWAARVYRPWAMVLVDRTHAKVVVSSLEEMRVVADETLDLDTSGW
                     RFGAGRPRTSTRATGVGSARGVARDRFDARVDELVHRFWQGIARTAADLSIDRVILAG
                     PEKGANAVRDFLPGAVQASVVGILALHSHSTIAAARAEGLPMIVAHELQQESRLLADL
                     RDRAGARAGGVLGLDATLGALARGEVMTLVGSRDLDASVWECPACSSVAAHPGACAAC
                     GGAVSRTSLRQILPVLARRHGARLEVVGPAAPPP"
     gene            1260..1694
                     /locus_tag="VEW91_09325"
     CDS             1260..1694
                     /locus_tag="VEW91_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYM91819.1"
                     /translation="MLRILVATDGSAHALKAAELAARLARETRGAEVMLVNVGHIPTI
                     ALGGPGDGLVDFAAMEDALEQAGRAILDQTNKAFAGVDARVTRVYRRGDPTAEILKAG
                     QEHKADLIVMGSRGLGQIGGLILGSVSERVLHGAHIPVLIVR"
     gene            1714..2568
                     /gene="deoC"
                     /locus_tag="VEW91_09330"
     CDS             1714..2568
                     /gene="deoC"
                     /locus_tag="VEW91_09330"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HYM91820.1"
                     /translation="MACDLVLRDTEVALWTVRRALERLPPAREIAHLARAADAELLAQ
                     ATVDDQPVPAPDAIPGIIDHTLLRADATPADIERVCREAREYAFASVCVHPRFVPLAA
                     RMVWGSGVRVSTVIGFPLGASTTVVKALEAVVAIGQGAQELDMVLPLGALKAGALEEV
                     GLEIAAVVAVGHAQDALVKVILETGTLSPAEQVRGCLLAREAGADFVKTSTGFVARGA
                     EERDVRRLRSLAGPRMGVKASGGIRTRTDLERMVRAGASRVGTSSGVEIIRAYREAGA
                     AVLAGRVA"
BASE COUNT          387 a          916 c         1005 g          372 t
ORIGIN      
        1 cgggggcggt gcggcggggc ccaccacctc gagccgggcg ccgtggcgcc gggccagaac
       61 gggcaagatc tggcggagcg atgtgcgaga aactgccccg ccgcaggccg cgcaggcacc
      121 ggggtgcgcc gcgacggagg agcacgccgg gcactcccag accgaggcgt ccagatcccg
      181 gctgccgacc aacgtcatca cctccccgcg cgccaacgcc ccgagcgtgg cgtccagccc
      241 cagcaccccg ccggcacgcg ccccggcgcg atcccgcaga tcggccagga gacgcgactc
      301 ctgctggagt tcgtgcgcca cgatcatcgg gagcccttcg gcgcgggcag cggcgatggt
      361 ggagtgcgag tgcagcgcga ggatgccgac gaccgatgcc tgcacggccc ccgggaggaa
      421 gtcgcgcacg gcgttcgccc ctttctccgg cccggcgagg atcacgcggt cgatcgagag
      481 gtcggcggcg gtccgggcga tcccctgcca gaaccggtgg acgagctcgt cgacgcgcgc
      541 gtcaaaccgg tcccgcgcga ccccccgggc tgacccgacg cccgtggcgc gcgtggaggt
      601 gcgcggtcgg ccggccccga aacgccagcc actcgtgtcc aggtccagcg tctcgtctgc
      661 gaccacccgc atctcctcga gagacgacac gaccaccttg gcgtgcgtcc gatccaccag
      721 caccatcgcc catggtcggt atacacgggc cgcccagagc agggggagga ggtcgggccg
      781 gccgtacagg atgcggttgg gcacgggatc ggggagcgcg tactcgcccc acaggtccgg
      841 cgcggccaga aagaccaggc cgtgcccggg aacaggcccg gccgtcaggc gcccctggat
      901 gcggtgcgcc gccgcgccga tgtcgcgccg ggccgccccg ggcgccgcct ccagcacccg
      961 cgcaagggcc tgatgcaccc agatccggta cgcgggattg ggggtttggt gctcgggctt
     1021 cgtggggtcg acgtccaggg cgacggaaag gacgtcccgg cggtcggcgt cgatcagctg
     1081 gggcaactcg cgcagggctg ccatcgcatg ttctagccta cccgttgccg ctgcggaatc
     1141 ccatccgttc gccgccgtat tctcctcggg aaatcctccc tcgacccgat atcaggctgg
     1201 gtgggggcgc cgctacgatt gaggtgcggg gggtagaagg ggaggaggtt gacccaaaga
     1261 tgctgcggat actcgtggcg accgatggtt cggcacacgc actcaaggct gccgagctgg
     1321 ccgcccgcct ggcccgcgag acccgggggg cggaggtgat gctcgtcaat gtcgggcata
     1381 tcccgacgat cgcgctcggg gggccggggg acgggctggt cgatttcgcc gccatggagg
     1441 acgccctgga acaggccggc cgggcgatcc tcgaccagac gaacaaggcg tttgccgggg
     1501 tcgacgctcg cgtcacacgc gtctaccgcc ggggcgatcc caccgcggag attctcaagg
     1561 cgggccagga gcacaaagcg gacctgatcg tgatgggcag ccgcggtctg ggccagatcg
     1621 gcgggttgat cctcgggagc gtgagcgagc gggtcttgca cggcgcccac atcccggtcc
     1681 tgatcgtgcg ttagaaagga ggctcctgtg ggcgtggcat gtgaccttgt ccttcgagac
     1741 accgaggttg ctctgtggac ggtccggcgg gccctggagc gcctgccccc ggcgcgggag
     1801 atcgcccacc ttgcccgcgc ggccgacgcc gaactgctgg cccaggccac cgtggacgac
     1861 cagccggttc cggcacctga cgcgatcccc gggatcatcg accacaccct gctgcgggcc
     1921 gatgcgaccc cggcagacat cgagcgcgtg tgccgggagg cacgcgagta tgccttcgcc
     1981 tcggtgtgtg ttcaccccag gtttgtgccg ctggccgccc ggatggtctg gggctccggg
     2041 gtgcgcgtct ccacggtgat cgggttcccg ctgggagcgt cgaccaccgt ggtgaaggcc
     2101 cttgaggccg tcgtcgccat cgggcagggg gcgcaggagc tggacatggt gctccccctg
     2161 ggggcgctca aagccggggc cctggaagaa gtcgggctgg agatcgccgc ggtggtcgcg
     2221 gtcgggcacg cccaggacgc gctggtaaag gtcatcttgg agacggggac gctgtcaccg
     2281 gccgagcagg tgcggggttg cctgcttgcc cgcgaggccg gcgcggattt cgtcaagacc
     2341 tccacaggat ttgtcgcccg cggggcggag gagcgggatg tgcgccgcct gcggtcgctg
     2401 gccggtccga ggatgggggt gaaggcctcg ggcgggatac gcacgcgaac cgacctggag
     2461 cgaatggtgc gggctggcgc cagccgcgtg ggcaccagca gcggtgtgga gatcatccgc
     2521 gcgtatcggg aagccggggc cgcggtcctc gccggcaggg tggcctaaac gctccgcatc
     2581 ccgcttggcg ctgcggagga cttccgccgc tcgcgctcga actcctcgcg ggtgaggtcg
     2641 ccctcctcga atcggtgcca cagctcatcc acgcggggat
//
LOCUS       DASQGH010000477         5807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36091, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000477 DASQGH010000000
VERSION     DASQGH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36091"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1886
                     /locus_tag="VEW91_09335"
     CDS             <1..1886
                     /locus_tag="VEW91_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYM91821.1"
                     /translation="LCRWTMSLTDLLFGRPLASAEQRAEKCGPGAGIAVFGLDALSSA
                     AYGPEAALTLLIPLGVAGIAYILPISASIIVLLAIVYFSYRQTIEAYPTGGGSYTVAR
                     ENLGPLPGLLAAAALMIDYTLTAAVGISAGVGALVSAVPRLQPHTLGLCLSILVLITI
                     VNLRGVRQVGAVFMLPTYLFVGSLLGAIALGLFKALAEGGHPVPVAAPPALHPQAAVL
                     SAWLLLQSFSNGCTAMTGVEAVSNGVRAFRDPVVKTAQRTLTVIITILAVLLAGIAYL
                     VRVYRIGATDPGAPGYESVLSQLVGAVAGRNAFYYITMASILLVLALSANTAFADFPR
                     VCRAVAQDGYLPHLFASRGRRLVYSHGIYVLAGLCAFLLILFGGVTDRLIPLFAVGAF
                     LAFTLSQTGMVMHWKRAGGRKSRPSMLVNGLGAVATALTVVVVVVAKFVAGAWVTVML
                     IPAMIFLMVSVRRHYDRVAAETASSTPLDVSHLRPPLVVMPVRGWDKVAQKALRFALN
                     ISPEVHVVHIDCGEDTENLRRKWSALVDLPTKRAGLATPQLVVLKSPYRYVITPILDY
                     VLDLERRHPDRQVAVLIPEMVERHWYHYLLHNQRAQWLKALLLLKGNQRINVISVPWY
                     LNE"
     gene            1955..2105
                     /locus_tag="VEW91_09340"
                     /pseudo
     CDS             1955..2105
                     /locus_tag="VEW91_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611171.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2110..2802)
                     /locus_tag="VEW91_09345"
     CDS             complement(2110..2802)
                     /locus_tag="VEW91_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYM91822.1"
                     /translation="MGADGPRILVVDDERAIRRFLRASLTAHGYQIFEASTAREGLEA
                     TTTVRPDLIILDLGLPDEDGIDVTRRLREWSKVPIVILSVRGQDEDKIAALDAGADDY
                     LTKPFSAAELLARIRVALRHAARPAEEPVITSGDLVVDVAHRVVTLAGREIALTPIEY
                     LLLKTLALHGGKVLIHRHLLREVWGPGYDQDTNLLRVNISKLRHKVESDPARPRYIIT
                     EPGVGYRLRMVN"
     gene            complement(2775..4796)
                     /locus_tag="VEW91_09350"
     CDS             complement(2775..4796)
                     /locus_tag="VEW91_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM91823.1"
                     /translation="MRSYMDVHAIPGPWPAGERVLVCIDADPSGERLVRTGRRMATGL
                     DAEWIVLHVETSEERALSERDQDRIARVLRLAEELGGRTVTLPGGSAAEEIIRYARSQ
                     NVTKIVVGVSHRPRWVQWFHGSVVDRVIRASGDIDVYVISGPTGARQPSRLSWEGRRE
                     DRGYPLVSYLYGLGVVGLATLAGALVREVLAPANLTMLYLLGVVVVAFQWGRGPAVVA
                     AALSVAAFDFFFVLPKVPFAGSDPQYLLTFAAFLAVGMVISGLASRAHEQAQAAQRRA
                     MYTAELHGLSAELAATSGTDAIVQAIARHVTATFARGVALFLPVHDELRPRFSSPGFP
                     LEANEHAVATWVFRHGQPAGYGTDTLPAARARYLPLKTARQIVGVLGVQPLGADSPLN
                     ADQRRLLDAFANQGAVALERAELAEEARRGHVLRETERLQTALLNSISHDLRTPLASI
                     TGALSSLVDGTAPVDESTRRELLENAKEEADRLNRFLGNLLDMNRLEAGALTPRIEPG
                     DVEDLVGTALTQLGDAAQQRDIRVSLPSPLPLVPMDFGLVVQALVNVLDNALKYSSPT
                     TPVDLRAEVTGDEVQIRTEDRGVGVPPNDLKRIFDKFYRVQREGAGGGVGLGLAISKG
                     IIEAHGGRIWAENRPGGGTTVVITLPLASPTSMQRSNGSTAWALTDRAS"
     gene            complement(4818..5423)
                     /locus_tag="VEW91_09355"
                     /pseudo
     CDS             complement(4818..5423)
                     /locus_tag="VEW91_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
     gene            complement(5520..>5807)
                     /locus_tag="VEW91_09360"
     CDS             complement(5520..>5807)
                     /locus_tag="VEW91_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HYM91824.1"
                     /translation="IDAVKQRVAALRAADPGNTAPVPVDLVTASASGLDPHISEAAAF
                     YQVPRVARVRRLSEETVRRLVQQHAEGRQFGVLGEPRVDVLELNRALEQAR"
BASE COUNT          912 a         1905 c         1917 g         1073 t
ORIGIN      
        1 tactatgcag gtggacaatg tcgctcaccg atctgctgtt tggaagaccg ctcgcttcag
       61 cagagcaacg cgcggagaag tgcgggccgg gcgctggaat agcggttttc ggtctagacg
      121 cgctcagctc cgccgcctat ggtccggagg cggcgcttac attgctcatt ccgctcggcg
      181 ttgccggcat tgcctatatc cttcccatta gcgccagcat catcgttctg cttgcgattg
      241 tctatttttc gtatcgccag accatcgaag cctacccaac cggcggaggg tcgtacacag
      301 tcgcccggga gaaccttggc ccgctgccgg gattgctggc ggccgcggcc ctcatgatcg
      361 actacacgct gaccgcggcc gtcgggattt cggccggcgt gggcgccctc gtgtccgcgg
      421 tcccccggct gcagccgcac accctagggc tatgcctctc catattggtc ctcatcacca
      481 tcgtgaattt gcgcggagtt cgccaggtcg gcgccgtgtt catgttgccg acctatctgt
      541 ttgtggggag cttgctcggc gcgatcgcgc tgggattgtt caaagcactg gctgagggcg
      601 ggcatccggt gcctgttgct gctccacccg ccctccatcc gcaggcggcc gtcctgagcg
      661 cgtggctcct gctgcagtca ttttcgaacg gctgtaccgc gatgaccggg gtggaggcgg
      721 tcagcaacgg cgtgagggca ttccgcgacc cggtggtgaa gacggcgcag cgcacgctga
      781 ccgtgatcat caccatcttg gcagtgctgc tggcgggcat cgcctacctt gtccgagtgt
      841 atcgcatcgg cgcaaccgac ccaggcgcgc cgggatatga aagcgtgctc tcgcagctcg
      901 tcggtgcggt cgcggggagg aatgcgttct attacatcac gatggcttcg attctgttgg
      961 tcttggcgct ctccgcgaac accgcctttg cagacttccc gcgggtgtgc cgggccgtcg
     1021 cgcaggacgg ctacctccca cacttgttcg cttcgcgcgg gcgacggctc gtgtactctc
     1081 acgggatcta tgtgctcgcg ggattgtgcg cgtttctgct tattttgttc ggtggcgtga
     1141 ccgacaggct gatcccgctc ttcgcggtgg gagccttcct ggcgttcacg ctttcgcaga
     1201 ccggcatggt gatgcactgg aaacgggctg ggggtcggaa atcgcgaccc agcatgctgg
     1261 tgaatgggct cggcgcggtg gcgacggcgc tcacggtggt ggtggttgtt gtcgccaagt
     1321 tcgtcgcggg cgcctgggtg acggtcatgt tgattccggc gatgatcttc ttgatggtgt
     1381 ctgtccgccg tcactacgac cgagtcgctg cagagactgc gagttcaacg cccctggacg
     1441 tcagccacct ccgtccgccg cttgtcgtca tgccggtgcg cggctgggat aaggtcgcgc
     1501 agaaggcgct gcgctttgcg ttgaacatct cgcccgaggt gcacgtcgtg catatcgatt
     1561 gtggagagga tacggaaaac cttcgccgga aatggtctgc gctcgtcgat ttgccgacga
     1621 aacgggcggg gctggcgacg cctcagctcg tggtgctcaa atcgccgtac cgctacgtga
     1681 tcactccgat tctggattat gtgctcgacc tggagcggcg gcatccggac cggcaggtgg
     1741 ccgtgctcat ccccgaaatg gtcgaacggc attggtacca ttacctcctg cacaaccaac
     1801 gcgcgcaatg gctgaaagcg ctgctgctgc tcaagggcaa tcagcggatc aacgtgatca
     1861 gcgtgccgtg gtatctgaac gagtagtttt tcttccgtga tgagtctcgg aagacggaaa
     1921 ctgccggcgg agcggcctgc gccggcggca cccggcgcgg cgctgggtcg tggagcgcac
     1981 cggcccctgg cacaaccggt tccgcaaact cctcgtccga ttcgagaaag cgcgtcgaga
     2041 actatcttgg attggcgcat gtggcatgct gccttatcgt ctaccggcta acggttttgg
     2101 gataggctct tagttcacca tgcgtagcct gtagcccacg ccgggttcgg tgatgatgta
     2161 ccggggacgg gcggggtcgg actcgacctt gtggcgcaat ttgctgatgt tcacgcgcag
     2221 cagattggta tcctgatcgt accctgggcc ccacacctca cgaagcaggt gccggtgaat
     2281 gagcaccttt cccccgtgga gcgcgagcgt cttcaggagc aggtattcaa tcggggtgag
     2341 cgcgatctcc cgcccagcca gggtgaccac gcgatgcgcc acatccacaa ccagatcgcc
     2401 ggaggtgatg accggctcct cggccggccg ggcggcgtgc cgcagggcca cacggatgcg
     2461 ggccaggagt tcggccgcgc tgaacggctt ggtcaggtag tcgtccgccc cggcatccag
     2521 ggccgcgatc ttgtcctcgt cctggccccg cacggagaga atcacaatgg ggacctttga
     2581 ccactcgcga agccgccggg taacgtcgat gccatcctcg tccggcaggc cgagatccag
     2641 gatgatcaga tcagggcgga cggtggttgt ggcctcgagg ccctcgcgcg ccgtggaggc
     2701 ctcgaagatc tggtacccgt gcgcggtcag cgatgcgcga agaaaacgac ggatggcgcg
     2761 ctcatcgtcg acgactagga tgcgcggtcc gtcagcgccc atgcagttga tccgttgctc
     2821 ctctgcatgc tcgtggggga cgcaagcggc agcgtgatga cgaccgtcgt cccgcccccc
     2881 ggacggttct ccgcccatat ccgtccgccg tgggcctcga tgatgccctt gctgatcgcc
     2941 aagccgagcc cgactccgcc cccggcaccc tcacgctgga cccggtagaa cttgtcgaag
     3001 atccgcttca aatcgtttgg aggaacgccc acgcctcggt cctcagtccg gatctgcacc
     3061 tcgtcccccg tcacctcggc gcgaaggtcg accggtgtcg taggagacga atacttgagc
     3121 gcgttgtcca ggacgttgac gagggcctgg accacaagcc caaagtccat cggcaccagc
     3181 gggagtggag acgggaggct gacacggata tcccgctgct gcgccgcgtc gccgagctgg
     3241 gtcaatgcgg tcccgaccag atcctccaca tcgcccggct cgatgcgggg ggtcagcgcg
     3301 ccggcctcca gccggttcat gtccaggagg ttgccgagaa agcggttcag gcggtcggcc
     3361 tcttccttgg cgttttccag gagctcgcgg cgggtggatt catccaccgg agccgtgccg
     3421 tccacgaggc tgctcagcgc gcccgtaatg gaggcgagcg gagtgcggag gtcgtgcgag
     3481 atggagttga gcagggcggt ctgcaaccgc tcggtttcgc gcaggacgtg cccgcggcgg
     3541 gcctcctcgg ccagctctgc ccgttcgagg gcaacggccc cctggttggc aaatgcatcg
     3601 agcaggcgcc gctggtcggc gttgagcggg ctgtcggctc ccaagggttg gacgcccagg
     3661 accccgacga tctgtcgcgc cgtcttgagc ggcagatagc gagcccgggc agcgggcagc
     3721 gtgtcggtgc cgtatcccgc cggctgcccg tggcggaaca cccacgtagc gaccgcgtgt
     3781 tcgttggcct ccagcgggaa tcccgggctg gagaatcgtg gtcgaagctc gtcgtggaca
     3841 gggagaaaca gggcgacgcc tcgcgcaaac gtcgcggtca cgtggcgggc gatggcctgc
     3901 acaatggcgt cggtgccact ggtcgcggcc agttctgcgc tcaagccgtg aagctccgcc
     3961 gtgtacatcg cacggcgctg ggccgcctgc gcctgttcat gcgcgcggct ggccaacccg
     4021 ctgatgacca tccccacagc gaggaaggcg gcgaacgtca gcagatattg ggggtccgac
     4081 cccgcgaacg ggaccttggg cagcacgaag aagaagtcga acgccgctac gctcagcgcc
     4141 gccgcgacca ccgccggacc ccgtccccac tggaaggcga cgacgacgac gcccagcagg
     4201 tacagcatgg tcaggttggc cggggcgagg acctccctga caagcgcgcc ggcgagcgtg
     4261 gcgagcccaa ccaccccgag accgtacagg tacgacacga gcgggtaacc tcgatcctcc
     4321 cgccggccct cccagctgag gcgcgatggc tggcgcgccc cggtgggtcc gctgatgacg
     4381 tacacatcga tgtcgccgct cgcccggatg acccggtcga cgacggaccc gtggaaccac
     4441 tggacccatc ggggacggtg tgagacgccc accacgatct tcgtgacatt ctgcgagcgt
     4501 gcgtaccgga tgatttcttc cgcggcgctg ccacccggga gggtgaccgt gcgccccccg
     4561 agttcctcgg ccagccggag cacgcgcgca atcctgtcct ggtccctctc ggacagcgcc
     4621 cgctcttcgc tcgtctcgac gtggagcacg atccactccg catccaaccc ggtcgccatc
     4681 cgccgtcccg tccgcacgag gcgttcgccc gacgggtccg catcgatgca gaccagcacc
     4741 cgctctcccg cgggccaggg gccggggatc gcgtggacgt ccatgtaact ccgcatgtcg
     4801 tggtccacgc ggcttgcgag ataccggagc gcgagctccc ggagcgccgt caggttcccc
     4861 ggccggaaga atttctcgat cgcgcggctc gcctgatcgg ggacgtagac cttgccgtcg
     4921 cgcaggcgct ggatcagttc gtcgggagga aggtcgacca gcccggtctc ggcagcctct
     4981 tccagagtat ggtcggggat ggtctcccga acggtcacgc cggtgatctg cgccacaata
     5041 tcgtttaggc tctcgatgtg ctgcacattc agcgtcgtgt agacgtcgat gccggcatcc
     5101 agcagttctt cgacatcctg gtagcgccgg gcgtgcctcg agccgggggc gttggtatgg
     5161 gccagttcgt ccaccagcgc cacctcggga cactggtcga ggacggcatc gaggtccatc
     5221 tcctccagtg tcactccccg gtacgcaatc tggcggcggg ggatcagggg aaggtcggcg
     5281 aggagggcct ccgtctccgg acgtccgtgg gtctcgacgt aggcgagggc cacacggcgt
     5341 ccctttgcca cttcttcccg ggccgcctgc agcatcgcat aggtcttgcc aacgcctgcc
     5401 gcatagccca ggaagatcct gagcttcccc ttcttgcggc ggcgctcttc ggtctcggcc
     5461 tgcttgagga gtgcctcagg acgcggacgt tcgggttcgc ctacgacagt aacggtccct
     5521 cacctcgcct gctccagtgc ccgattcaac tctagcacat ccacgcgagg ttcgccgagg
     5581 acaccgaatt gacgcccttc cgcgtgttgc tggacgagcc gccgtaccgt ctcctccgaa
     5641 agccggcgca cgcgggcgac gcgcggcacc tggtagaagg cagcagcctc actgatgtgg
     5701 ggatccaggc cgctcgccga cgccgtcacg aggtccaccg ggacgggtgc cgtgttcccc
     5761 ggatcggccg cccgcagggc ggccacccgc tgcttcaccg catcgat
//
LOCUS       DASQGH010000478         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52953, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000478 DASQGH010000000
VERSION     DASQGH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52953"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VEW91_09365"
     CDS             complement(<1..841)
                     /locus_tag="VEW91_09365"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYM91825.1"
                     /translation="MSALAGPPLPQPTRLRRFLRHLDILLVVSTVALVGFGLVMVYSS
                     THLGPRPFLYLRSQALHLAAGLVIAGLILAIDYRVLASSARLLYVGNLVLLGAVLIVG
                     RTSLGAQRWLALGPFGQFQPSELAKLIIVITLAKHLTDRPGPYRSVWDLAPFLGHIAL
                     PMLLIFRQPDLGTALVLGAIFVGMLYAAGARRRDLATLGVAGAALVPILWHVLKEYQR
                     RRLLAFVHPDLDPLGSGYGIIQSKIAVGSGLAWGKGLFAGTQGVLRFVPEHHTDFIFS
                     VIGE"
     gene            complement(838..2601)
                     /gene="mrdA"
                     /locus_tag="VEW91_09370"
     CDS             complement(838..2601)
                     /gene="mrdA"
                     /locus_tag="VEW91_09370"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYM91826.1"
                     /translation="MDRDIFERRLTVLLAVIGGLLLVLAVRLWQIQIVQGEYFLRLSE
                     ENRIRISSIVAPRGLIKDRRGRPVILNRPAFTVYLLPTELRNPEQVIAEVAGKLGMTT
                     AEVAAKLAAAARERPFDPVLLRRDVPKEAVAALEENRMDLPGVLVQVEPVRDYLHGTL
                     AAHLLGYVGEIDDRDLAALRRQGYEAGELIGKDGVERIYDRYLKGRNGQIQDEVDAEG
                     RPLRVLATVPPSPGNTVVLGIDLDVQQAAEDALGSRVGAVVAMDPNDGTIVALASHPA
                     FDPNLFAIGITGSAWTHLLHDPLQPLLDRATQGDYPVGSVFKIVTATAALELGLVQPQ
                     TRFYCPGFYILGNRIFHDHEAHGDLTFFEGIAQSCNVVFWTLARPVGPDQLARFARMY
                     GLGQTTGIDLVQESAGIIPDPQWKLRVWKEPWYPGDTLNSAVGQGFTLATPIQIARLV
                     AAVANGGRLVTPHIATEVTSPQGQVLLRVAPPAAGTIHVSPQTLAVLQSALVAVVTHG
                     TAASIQIPGLAVAGKTGTAENPHGRPHAWFAGYAPATTPQLVVVALVENVGYGAEFAA
                     PIVRRVLQVAFGLPAGASAHP"
     gene            complement(2655..3161)
                     /gene="mreD"
                     /locus_tag="VEW91_09375"
     CDS             complement(2655..3161)
                     /gene="mreD"
                     /locus_tag="VEW91_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYM91827.1"
                     /translation="MIRYAAYLLLVAAGLVLEMTWLPRATIAGAAPDPLIIFVMVVGL
                     LHGSEEGALVGAGVGLLQDVVTGVPLGLGMLADLMVGFAAGLGERVIYIESAWLPAVS
                     ATALSGLRAGVWALAAHLVGLLGGPMLEVVRVVVATACYNGIIAVPIFHGLRSLEGAL
                     VRFHEESR"
     gene            complement(3158..3997)
                     /gene="mreC"
                     /locus_tag="VEW91_09380"
     CDS             complement(3158..3997)
                     /gene="mreC"
                     /locus_tag="VEW91_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYM91828.1"
                     /translation="MLLDAFARRRRFVVFVALCILALGVLTDQVRSPEQRRIGWLGAA
                     VEVALAPAAAGLSRVSEVVDRGWALLTEIGQLRVENRRLNAEVARLREENARLQEDAQ
                     AAQRLRSLLAFKQPSYRTVSARVIGRDPSHWFNTLLIDRGPEDRIRRNDPAVTSEGLV
                     GHIIETSGSWARVLLILDPRSAVGVLVGQSRDAGVAEGQGQPILRVKYLSRDAEIHPG
                     DQVVTAGLGEIYPRGLVVGTVVGVARTDGDLFQEAMVRPAADLDHLEEMLILVSEGSQ
                     TSR"
     gene            complement(4006..>4463)
                     /locus_tag="VEW91_09385"
     CDS             complement(4006..>4463)
                     /locus_tag="VEW91_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYM91829.1"
                     /translation="IIQYARKAYNLLIGERTAEDIKIAAGSAFPQREEQTIEVRGRDL
                     VSGLPRTVRMTSTEIREAMAEPIAGIVEAVKMTLERTPPELAADIVDRGIVMAGGGSL
                     LRGLDRLLAEETGMPVTLTDDPLSAVVLGTGKALEEIDTLKKVLITSKK"
BASE COUNT          753 a         1573 c         1509 g          628 t
ORIGIN      
        1 cctcgccgat caccgagaag atgaagtcgg tgtgatgctc gggcacgaac cggaggaccc
       61 cctgggtccc ggcaaacagc cctttgcccc acgcgaggcc gctgcccacg gcgatcttcg
      121 attgaatgat gccgtagcca gatcccaagg gatccaggtc cgggtgcaca aaggccaaca
      181 gccgccgccg ctggtactcc ttgagcacgt gccagaggat cgggaccagg gccgctcccg
      241 ccacccccag cgtggcgagg tcccgccggc gcgcaccggc cgcgtagagc atcccgacga
      301 agatggcgcc caggaccagc gccgttccga gatccggctg cctgaagatc agcagcatcg
      361 gcagcgcgat atggccgagg aagggcgcca gatcccagac cgaccggtac ggtccggggc
      421 ggtcggtgag gtgcttggcc agcgtgatca cgataatgag cttggcgagc tccgacggct
      481 gaaactgccc gaacggcccg agcgccagcc agcgctgggc gccgaggctc gtccggccca
      541 cgatcaggac cgcgccgagg agcacgaggt tccccacata aagcagccgg gcgctcgacg
      601 ccagcacccg gtagtcgatc gcgaggatga gccccgcgat cacgaggccg gccgcgaggt
      661 gcagcgcctg gctccgcagg tagagaaacg gccggggtcc gaggtgggtg ctgctgtaca
      721 ccatgacgag gccgaacccc acgagcgcca ccgtcgagac caccagcagg atatccagat
      781 gccggaggaa ccggcgcagg cgggtcggct ggggaagcgg gggaccggcc agcgcgctca
      841 cgggtgggcc gaggcgccgg cgggaaggcc aaacgccacc tgaagcaccc gccggacgat
      901 gggggcggcg aactccgcgc cgtaccccac gttttcgacg agggccacaa ccacgagttg
      961 gggggtggtg gccggggcgt agccggcgaa ccacgcgtgc ggcctgccgt gcgggttctc
     1021 tgcggtcccg gtcttgccgg cgaccgccag ccccgggatc tggatcgacg cggcggtgcc
     1081 atgggtgacc acggcgacca gcgccgactg gagcaccgcc agcgtctgcg ggctcacgtg
     1141 aatcgttccg gccgcgggcg gcgcgacacg gaggagcacc tgcccctggg gcgaggtgac
     1201 ctcggtcgcg atgtgggggg tgacgagccg gcccccgttc gccacggcgg ccacgagccg
     1261 ggcgatctgg atgggggtgg ccagggtgaa tccctgaccg accgcggagt tcaacgtgtc
     1321 cccggggtac cacggctcct tccacacgcg gagtttccac tggggatcgg gaatgatccc
     1381 cgcgctttcc tggacgaggt cgatccccgt cgtctggccg aggccgtaca tcctggcgaa
     1441 ccgggccagt tggtcggggc ccacggggcg ggccagggtc cagaacacca cgttgcacga
     1501 ttgggcgatg ccctcgaaga aggtgaggtc gccgtgcgcc tcgtggtcat gaaagatgcg
     1561 gttgccgagg atgtagaacc cgggacagta gaaccgggtc tgcggctgga ccagtcccaa
     1621 ttccagggcg gcggtcgccg taacgatctt gaacaccgag cccactggat aatcgccctg
     1681 ggtggcgcgg tcgagcagcg gttgcagcgg gtcgtgcagc aggtgcgtcc acgccgaacc
     1741 ggtgattccg atcgcaaaga ggttggggtc gaacgcagga tggctggcga gggcgacgat
     1801 cgtgccgtca ttgggatcca tcgccacaac ggctccgacc cgcgagccga gggcatcttc
     1861 cgccgcctgc tggacatcca ggtcgatccc cagcaccacg gtgttgccgg gggacggcgg
     1921 gaccgtcgcc agcacccgca gcgggcgccc ctcggcatcg acctcgtcct ggatctgccc
     1981 gttgcgcccc ttgaggtagc ggtcgtagat ccgctcgacc ccgtcttttc cgatcaactc
     2041 accggcttca tagccctgcc ggcgcagcgc cgcgagatcc cggtcatcga tctctcccac
     2101 gtaccccaac aggtgcgcgg ccagggtgcc gtggaggtag tcccggacgg gctccacctg
     2161 gacgaggacg ccggggagat ccatccggtt ctcctcgagc gccgcgacgg cttccttcgg
     2221 gacatcgcgc cgcaggagga ccggatcgaa cggccgctcc ctggccgcgg ccgccaactt
     2281 ggccgccacc tcggccgtgg tcatgccgag cttgccggcc acttcggcga tcacctgctc
     2341 aggattccgc aactcggtgg gcagcaggta gacggtgaag gcggggcggt tcaggatcac
     2401 cgggcgcccg cggcggtctt tgatcagccc gcggggcgcg acgatgctgc tgatgcggat
     2461 ccggttttcc tcggagagcc ggaggaagta ctctccctgg acaatctgga tctgccagag
     2521 gcgtactgcc agcaccagca acagcccgcc gataaccgcc agcagcacgg tcaggcggcg
     2581 ttcaaaaatg tctcggtcca tcgcggccgg gccccctcgc gcgagggcgc aggccggcat
     2641 ccggggagcg acccttagcg ggactcctca tggaaccgga caagcgcccc ctcaagggag
     2701 cgaagtccat ggaaaattgg taccgcaatg atgccattat agcacgccgt cgccacgacc
     2761 acccggacga cctcgagcat cggccccccg agcaggccca cgagatgcgc ggccagggcc
     2821 cacaccccgg cccgaagccc gctcagggcc gtcgcactga ccgcgggcag ccacgcgctt
     2881 tctatatata tgacgcgttc cccgaggccg gccgcgaagc ccaccatgag atcggccagc
     2941 atcccgagcc ccagcggaac gcccgtcacg acgtcctgga ggagcccgac ccccgcgccc
     3001 acgagggcgc cttcctcaga gccgtggagc agccccacga ccatcacgaa gatgatgagc
     3061 ggatccgggg ccgcccccgc gatggtggcc cgtggcagcc acgtcatctc cagcaccaat
     3121 cccgcggcga cgagcagcag gtaggctgcg tatcgaatca tcgggaagtc tgcgatcctt
     3181 ccgagaccag gatgagcatc tcttcgaggt ggtcgaggtc cgccgccgga cggaccatcg
     3241 cctcctggaa cagatcccca tcggtccggg ccacccccac gaccgtgccc accacgagcc
     3301 cgcgcggata gatctcgccg agcccggccg tcaccacctg gtccccgggg tggatctcag
     3361 cgtcgcgaga cagatacttg acgcggagaa ttggctggcc ctggccctcg gccaccccgg
     3421 catcgcggga ctggccgacc agaaccccga cggcgctgcg ggggtccagg atcagcagca
     3481 cccgcgccca cgaacccgac gtctcgatga tgtggcccac caacccctcg ctcgtgaccg
     3541 cggggtcgtt gcgccggatc cggtcctcgg gcccgcgatc gatgagcagc gtgttgaacc
     3601 agtggctggg atcgcgcccg atcacgcggg cggagacggt ccggtacgac ggctgcttga
     3661 aggccagaag gctccggagc ctctgggcgg cttgggcgtc ttcctgaagt cgggcgttct
     3721 cctcgcggag ccgggctacc tcggcattca gccgccggtt ttccacccgg agctgaccga
     3781 tctccgtgag gagcgcccag cctcggtcca ccacctcgct gacccgggag aggccggccg
     3841 cagccggagc cagcgccacc tcgacggcgg cgccgagcca tccgatccgg cgctgttccg
     3901 gggaccgcac ctgatcggtc agcactccga gggcaagaat gcagagcgcg acgaaaacga
     3961 cgaatcggcg gcgtcgagca aacgcgtcga ggagcacagg ggggtctact tcttgctcgt
     4021 gatcagcacc ttcttcaggg tgtcgatctc ctccagcgcc ttcccggtcc ccagcaccac
     4081 agcgctcagc ggatcatcgg tgagcgtcac cggcatcccg gtctcctccg ccaacagccg
     4141 gtcgagccct cggagcagcg agcccccgcc ggccatgacg atcccgcggt caacaatatc
     4201 cgccgcgagt tcgggcggcg ttcgctccag cgtcatcttc accgcctcca cgatgccggc
     4261 gatcggctcg gccatcgcct cgcggatctc cgtgctggtc atccgcacgg tgcgcggcag
     4321 gcccgacacc agatcgcggc cccgcacctc gatcgtctgc tcttcgcgct gggggaacgc
     4381 ggagcccgcg gcgatcttga tgtcctccgc cgtccgctcc ccgatgagga ggttgtacgc
     4441 cttgcgggcg tactggatga tcg
//
LOCUS       DASQGH010000479         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47486, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000479 DASQGH010000000
VERSION     DASQGH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47486"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..950)
                     /gene="tpiA"
                     /locus_tag="VEW91_09390"
     CDS             complement(183..950)
                     /gene="tpiA"
                     /locus_tag="VEW91_09390"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYM91830.1"
                     /translation="MRTPLIAANWKMHMTIAQAVRLIEEVKAALGEFAGAEVVICPPA
                     TALAAVGQALAGTSFLLGGQTMHWEPRGAYTGEVSGPMLVDVGCRCVILGHSERRLYF
                     GETDETVGRKVRAAFEHGLTPIACVGERLEERQAGRTDAVVASQVRAALREVPGGPLH
                     RLVIAYEPVWAIGTGHTATGAEANRVAGVIRGTVAGSGGTGAASAVRILYGGSVKPDN
                     ILEFLEQPEIDGALVGGASLDAQAFAAIVRAAGRVAR"
     gene            complement(953..>1985)
                     /locus_tag="VEW91_09395"
     CDS             complement(953..>1985)
                     /locus_tag="VEW91_09395"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYM91831.1"
                     /translation="GAMVVLASHLGRPKGVDEALRMDPIARRLGELLHRPVRKLNDCV
                     GPSVASALQEMRPGDIVLLENLRFHPGEEANDPAFARALAAPVQLFVNDAFGTAHRAH
                     ASTVGVPSLVPAVAGFLMERELTYLGKVLEAPTRPLVVILGGKKVDDKIGVIRNLLRL
                     AQTMLIGGGMCYTFLRAAGARIGGSLCDEERLGLARELMAESDRRGVRLMLPLDVVAA
                     QRLAADAPVRVVDAREIPDGWIGLDIGPRTEAAFSAPIAAAGTILWNGPMGVFELPPF
                     AGGTRAIARAVAESCAESIVGGGDTASAVEQFGVAAQITHVSTGGGATLEFMEGKVLP
                     GVAALQDAA"
BASE COUNT          328 a          752 c          637 g          268 t
ORIGIN      
        1 ccgccaccga tcgaccccaa cccttcgccc ttgggcccct ggagcacgat cacgccaacc
       61 acggcgacgg caacgataaa gtgcaggatg agcaccgcac tagacacctg gacacctccc
      121 gcgctacctc cctaacagta ccacagcccc gccggaggag acaagaccgc tcccgggcgg
      181 ccctagcgtg cgacccttcc ggcggcccgc acgatcgcgg caaacgcctg ggcgtccagg
      241 ctggctcccc cgacaagggc gccgtcgatc tccggctgct cgaggaattc cagaatattg
      301 tcgggtttga cactcccacc gtacaggatg cggacggccg atgcagcgcc ggtgccgccc
      361 gatcccgcga ccgtcccccg gatcacgccc gccacacggt ttgcctccgc cccggtcgcg
      421 gtgtggccgg taccgatcgc ccagaccggt tcgtacgcga tgaccaaacg gtgcaggggg
      481 ccgcccggga cctcccggag tgcggcccgg acctgcgagg cgacgaccgc atcggtgcgc
      541 ccggcctgac gctcctcgag gcgctcccca acgcacgcga tcggggtgag accgtgttcg
      601 aaggccgccc gcacctttcg cccgaccgtc tcgtccgtct ctccgaagta gagccgccgc
      661 tccgagtggc cgaggatgac acagcggcac cccacgtcca ccagcatcgg cccgctcacc
      721 tcgccagtgt aggcgcccct gggttcccag tgcatggtct ggccccccaa gaggaaggag
      781 gtgcccgcca gggcctgtcc aacggcggca agggcggtgg cgggaggaca gatcaccacc
      841 tcggccccgg caaactcccc gagggcggcc ttcacctctt cgatcaggcg aacggcctgc
      901 gcgatcgtca tgtgcatctt ccagttggct gcgatcaggg gagtgcgcac gatcaggccg
      961 cgtcctgcag cgccgccaca ccgggcagga ctttgccctc catgaactcc agcgtggcgc
     1021 cgccgccggt gctgacgtgc gtgatctggg ccgcgacccc gaactgctcg accgcggacg
     1081 cggtatcgcc ccccccgacg atcgattcgg cacaggactc cgccaccgct ctcgcgatgg
     1141 cccgggtccc gccggcaaac gggggcaatt cgaacacacc catcggtccg ttccacagga
     1201 tcgtcccggc cgcggcgatg ggcgcgctga acgcggcttc ggtccgggga ccgatatcga
     1261 gcccgatcca cccatcgggg atctcgcggg catcgaccac ccggaccggc gcgtcggccg
     1321 ccaggcgctg ggccgcgacg acgtccagcg gcagcatcag gcgcacgccc cggcggtccg
     1381 actcggccat gagctcgcgg gcaaggccga ggcgctcctc atcgcacaac gagcccccga
     1441 tcctggcgcc cgcggcccgc aggaacgtgt agcacatccc gcccccgatg agcatcgtct
     1501 gcgcgagccg cagcaggttg cggatcactc cgatcttgtc atccaccttc ttgcccccga
     1561 gaatcacgac cagcggacgg gtgggggctt caaggacctt gccgagatag gtcagttccc
     1621 gctccatcag gaagcccgcg acggcgggca ccagcgacgg gacgcccacc gtgctggcgt
     1681 gcgcccggtg ggccgttcca aaggcgtcat tgacaaagag ctgcaccggg gcggcgaggg
     1741 cgcgggcaaa ggccggatcg ttggcctctt ccccaggatg gaaccgcagg ttttcgagga
     1801 ggacgatatc gcccggccgc atttcctgga gggccgacgc aacgctcggc cccacgcagt
     1861 cgttcagctt gcggaccggc cgatggagca actcccccag gcggcgggca atggggtcca
     1921 tccggagggc ctcatcgacc cctttcgggc gccccaggtg tgaagccagg accaccatcg
     1981 cgccg
//
LOCUS       DASQGH010000480         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52958, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000480 DASQGH010000000
VERSION     DASQGH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52958"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1557
                     /gene="proS"
                     /locus_tag="VEW91_09400"
     CDS             94..1557
                     /gene="proS"
                     /locus_tag="VEW91_09400"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HYM91832.1"
                     /translation="MSQPSVSQPAEDREHVKEIPSKSEHFGEWYTALILKAELADYYP
                     VRGCTVIRPYGYTIWELMQQGLDRRFKATGHTNAYFPLFIPQSFFHREAEHVKGFAPE
                     VAWVTRGGGEELAEPLAVRPTSETAIGHMYARWIRSYRDLPVLINQWCNVVRWEKATR
                     PFLRTMEFLWQEGHTAHRTPDEAEAEARQMLEVYRDFAETDLSIPVLAGRKPDSEKFP
                     GALHSYTIEAIMPDGQALQMGTSHNLGQNFARAFNIKFLDSDNTEKYVSTTSWGVSCR
                     MVGGLIMVHGDDRGLVLPPKVAPIQVVIVPILTGKAREEVLATARSLAERLTPDARVR
                     LDDRTEVTPGWKFNDWEMRGVPLRLELGPRDLSQRQVVLVPRVGGNRRSVPMDGAADA
                     VMSALREVQEALFRLAKGFLDAHIVSPSTLDEAVDAIASRKGFVRLGWCGAAACEEAL
                     QRATGASPRLIADERAADPCLACGTAARHVVYYARAY"
     gene            1615..2169
                     /locus_tag="VEW91_09405"
     CDS             1615..2169
                     /locus_tag="VEW91_09405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYM91833.1"
                     /translation="MLVAVLVLAAAAAALAFARLLPSGLPRPVGPAPGGNAPPVGRPA
                     PDFSLALLNGGTLAFHSLKGKPVLLNFWASWCAPCREETPLLVRLHKVYGPRGVEFVG
                     IDAEDQPADARRFIAEYHVDYAVAHADDERLIDAFEIPGLPTTVLIGADGIVVGKVVG
                     GFVGPEGEKLLIGWLDRLLAAAGR"
     gene            2282..2824
                     /locus_tag="VEW91_09410"
     CDS             2282..2824
                     /locus_tag="VEW91_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYM91834.1"
                     /translation="MMLAGALLVGCLGLSGCTGRDILSMRAHPETQPAAPGPATPAPP
                     FTGPALAATTDYPKNENAPRPEPPAEFASMKNPLEPTPDNLAKGKALFDANCAVCHGP
                     TGLGNGTAGGSLNPRPANFATPIHSALPDGYWFWRLSKGGGVPPFNTSGSAMPPWESA
                     LSADQRWLVILYEHTFRPHQ"
     gene            2821..3324
                     /locus_tag="VEW91_09415"
     CDS             2821..3324
                     /locus_tag="VEW91_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYM91835.1"
                     /translation="MTAGLTGGGQSAPERVRTALAARGLTVEIVEFSESTRTAEEAAR
                     AVGVTVGQIVKSLVFVADGRPVLVLTSGGNRVDLKKVARLAGASRVEKATAEMTRAAT
                     GFSIGGVPPVGHPTTLPVLVDEMLLRYAVVYASAGTPHAVFAIAPRDLERVTGGMVAD
                     VVEPARA"
     gene            3358..>4345
                     /locus_tag="VEW91_09420"
     CDS             3358..>4345
                     /locus_tag="VEW91_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYM91836.1"
                     /translation="MTILHVALVFLLGAALVSIIAERLRLPYTVALVIAGALAGLSRI
                     PPSVALTPDVLLTLVIPPLLFEGALRLSVEHLRTYAMLIGLLAIVGTIAAALAIAWAA
                     AVVGHLSVPTALLLGAIAAAIDPVSVIALIREAGLDARLGTILQAEAVFNDGVVVVLF
                     TILTGSAALVPLAAAGQFLWLLGVGGLVGLALGVGIAQGLGRVTTQPLVEALGSLILL
                     AASVVVAGSVGASGIVAAMCAGVVFTRYRERMLTEAGREIVGTLWDVIAFLANSALFL
                     FIGLQVPVALLVRYWGLIAVVVIAALAMRALVVYGFAALSERRGMPVPRPWRP"
BASE COUNT          592 a         1512 c         1547 g          694 t
ORIGIN      
        1 gtccggccgg actgggcagc agggagcgca tggaagttgc gggggcctcc cgtcccgtgg
       61 ggacgcggag gctcccggcc tgtaggaggc ggtatgtccc agccgtcggt gtcccagcct
      121 gccgaggacc gcgagcacgt caaggagatc ccgtccaagt ccgagcactt cggagaatgg
      181 tacaccgccc tcatcctcaa ggccgaactc gcggactact accccgtgcg cggatgcacc
      241 gtgatccgcc cgtacgggta caccatctgg gaactcatgc agcaggggct cgaccgccgg
      301 ttcaaggcga ccggccacac caacgcctac ttccccctgt tcatccccca aagctttttc
      361 caccgcgagg ccgagcacgt caaagggttc gcgccggagg tggcctgggt gacccggggc
      421 ggcggcgagg aactggccga gccgctggca gtgcggccca cctcggagac cgcgatcggg
      481 cacatgtacg cccgctggat tcggtcctat cgagacctgc cggtgttgat caaccagtgg
      541 tgcaacgtcg tgcgctggga gaaggccacc cggccgttcc tccggacgat ggagttcctc
      601 tggcaggaag gccacaccgc gcaccggacc cccgacgaag cggaggccga agcgcgccag
      661 atgctcgagg tgtaccggga ctttgcggag accgacctgt cgatcccggt cctggccggc
      721 cggaagccgg acagcgagaa gttccccggg gcactgcact cctacacgat cgaagcgata
      781 atgcccgatg gccaggcgct ccagatgggc acctcgcaca acctgggaca gaacttcgcc
      841 cgggcgttca acatcaagtt cctggacagc gacaacaccg agaagtacgt ttccacgacg
      901 tcgtggggcg tcagctgccg gatggtcggc ggccttatca tggtccacgg cgacgatcgg
      961 gggcttgtgc tgccgcccaa ggtcgccccg atccaggtgg tcatcgtgcc gattctcacg
     1021 ggcaaggctc gcgaggaagt gctggcgacg gcccgatcgc tcgccgaacg gctgaccccc
     1081 gacgcgcggg tcaggctgga cgaccggacc gaggtcaccc ccgggtggaa gttcaacgac
     1141 tgggagatgc ggggggtgcc gctgcggctc gaacttgggc cgcgcgacct cagccagcgg
     1201 caggtggtcc tcgtcccccg ggtcggtggg aaccggcggt ccgtcccgat ggacggggcc
     1261 gccgacgccg tgatgtctgc gctccgggaa gttcaggagg ccctgtttcg gctggcgaag
     1321 ggctttctcg acgcccacat cgtctcgccc tcgacacttg acgaagccgt cgacgcgatc
     1381 gcgtcgcgca aagggttcgt gcgcctcggg tggtgcggag ccgcggcatg cgaggaggcg
     1441 ctccagcgcg cgaccggcgc atcgccgcgc ctcatcgccg acgagcgcgc ggccgatccc
     1501 tgcctcgcct gcgggacggc ggcgcgccac gtggtgtact acgcccgcgc ctactgaccc
     1561 gggatgtgcg cgcagccgga tcccgttcct cgcaaccgcc gtgtccatct tcccgtgttg
     1621 gtggcggtcc tcgtgctcgc cgcggcggcc gcggcgctcg cgttcgctcg cctgctgccg
     1681 tccggtctgc cgcggccggt cgggccggcg ccgggcggca atgcgccacc ggtggggcgc
     1741 cccgcgccgg acttctcgct cgcgctgctg aacggcggga cgctcgcctt tcacagcctc
     1801 aaggggaaac ccgtcctcct gaacttctgg gcctcctggt gcgcgccgtg ccgggaggag
     1861 actccgctgc tcgtgcgcct gcacaaggtc tacggcccgc gcggcgtgga atttgtcggg
     1921 atcgacgccg aggatcaacc cgccgatgcg cggcggttca tcgcggagta ccatgtcgac
     1981 tacgcggtgg cgcacgcaga cgacgagcgg ctgatcgacg cctttgagat ccccggcctt
     2041 ccgacgaccg tgttgattgg ggcggacggg atcgtcgtgg ggaaggtggt gggcggcttt
     2101 gtcgggcccg aaggggagaa gctgctgatc gggtggctcg accgcctcct cgcggctgcc
     2161 gggcgctagg ggcgaacgca gggaaaacgg acagcacggc aaaacatacc cagggcctcg
     2221 catcgggatc tcccgcgctg cgatcctcgt cgcgatccgg gggtggtgga tgttgtcgcg
     2281 catgatgctg gccggggcac tgctggtcgg gtgcctcggg ctctccggct gcaccggcag
     2341 ggacattctg tcgatgcgcg cgcacccaga gacgcagccg gcggctcccg gacccgccac
     2401 cccggctccg cccttcacag ggcccgcgct cgccgccacg acggactacc cgaagaacga
     2461 gaacgccccc cgccccgagc cgccggcgga gttcgcctcg atgaagaacc cgctcgagcc
     2521 caccccggac aacctggcga aggggaaggc cctcttcgac gccaactgcg cggtgtgcca
     2581 cggcccgacc ggtttgggga acggcaccgc cgggggatcg ctcaacccgc ggccggccaa
     2641 cttcgcgacc ccgatccaca gcgcgctgcc cgacggctac tggttctggc ggctctcgaa
     2701 gggcgggggc gtccccccgt tcaacacgtc agggtccgcg atgccgccct gggagtccgc
     2761 cctcagcgcc gaccaacgct ggctggtgat tctctacgag cacacgtttc gcccgcacca
     2821 gtgacggcgg ggctcaccgg cggcgggcag tcggcgcccg agcgcgttcg gacggcgctc
     2881 gccgcgcgcg ggctgaccgt ggagatcgtc gagttctcgg agagcacccg caccgcggaa
     2941 gaggcggcgc gcgcggtggg ggtgacggtg ggccagatcg tcaaatcgct ggtgttcgtc
     3001 gcggatggaa gacccgtgct ggtcctgacc tcgggcggga accgcgtcga cctcaagaag
     3061 gtggcccggc tcgccggcgc ctcgcgcgtc gagaaggcca ccgccgagat gacccgcgcc
     3121 gcgacggggt tcagcatcgg cggcgtgcct cccgtgggcc atccgaccac tctgccggtg
     3181 ttggtcgatg agatgctgct ccgctacgcg gtggtctacg cctcggccgg caccccgcac
     3241 gcggtcttcg ccatcgctcc gcgcgacctg gagcgcgtga cggggggcat ggtggcggac
     3301 gtcgtcgagc cggcccgcgc ctagggcacg tctggtcagc ccgcaggcgt caggcggatg
     3361 acgatccttc acgtcgcgct ggtctttctc ctcggcgcgg cgctcgtgtc gatcatcgcc
     3421 gaacggctgc gactgcccta cacggtggcg ctcgtgatcg cgggcgccct cgcgggcctg
     3481 agccgcatcc ccccttcggt ggccctcacc ccggacgtgc tgctgacgct cgtgattccg
     3541 ccactgctct tcgaaggcgc gctgcgcttg tccgtcgagc acctccggac gtacgcgatg
     3601 ctcatcgggt tgctggcgat cgtcgggacg atcgcggccg ccctggcgat cgcctgggcc
     3661 gcggcggtgg tgggccacct ttcggtgccg accgccctcc tgctcggcgc gatcgcggcg
     3721 gcgatcgatc ccgtcagcgt cattgccctg atccgggagg ccgggctcga cgctcgcctg
     3781 gggacgatcc tccaagcgga ggccgtcttc aacgatggtg tagtcgtggt gctgttcacg
     3841 atcctgaccg ggtccgctgc attggtgccc ctcgccgccg ctggtcagtt cctgtggctg
     3901 ctcggggtcg gcggcctggt gggcctggcg ctgggcgtgg ggatcgccca ggggctcggc
     3961 cgcgtcacca cgcaaccgct ggtcgaagcg ctcggcagcc tgatcctgct ggccgcctcg
     4021 gtggtggtcg ccgggagcgt tggggccagc ggcatcgtcg cggccatgtg cgcgggcgtg
     4081 gtcttcaccc gttacaggga gcgaatgctg accgaagcgg gtcgtgagat cgtcgggaca
     4141 ctctgggatg tgatcgcgtt cctggccaac tccgcgctgt ttctctttat cgggctgcag
     4201 gtgccggtgg cgctgctggt tcggtactgg ggtctgatcg ccgtcgtggt catcgcggcc
     4261 ctcgccatgc gcgccctcgt cgtgtacggg ttcgcagccc tgagcgagcg acgcgggatg
     4321 ccggttcccc ggccgtggcg gccgg
//
LOCUS       DASQGH010000481         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16240, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000481 DASQGH010000000
VERSION     DASQGH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16240"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /locus_tag="VEW91_09425"
     CDS             complement(<1..1830)
                     /locus_tag="VEW91_09425"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HYM91837.1"
                     /translation="MDIVNPVVQRWLREAQEDVEGFWARAAEQLPWLRPWDRVFEWNF
                     PTFRWFVGGETNLAVNCLDRHVAAGRGGHAALIYLNERGERRLHTYAQLHHEVERTAA
                     ALRGLGIRKGDRITIYMPTCPEAIILMLAAVRIGAIHSVVFAGFGAGALGDRIRASGS
                     RLVFTADVAYRKGRDVPLWGIVADALRGPGHTVERVIMLSRGGTGGAQPGQALSWPEF
                     LACGAGHSGGSVPMEANEPAYILATSGTTAKPKLAVHTHGGYQVYIYSMGRWLFGMTP
                     GDVWWSTSDIGWVVGHSYIVYAPLLFGCTTVAYEGALDHPGPEVLWRAVEEFGVTGIF
                     TSPTGVRLLMRYGEEPTRNCNLSSLERVFCAGEVLNPPAWEWLQTRVLGDRIPVIDHM
                     WQTETGGPVFGNPYGLGMLPIKPGSAGIPLPGIEAAVVTPDGRPCPPNEKGIMVIKRP
                     FPGLIQTLWGEPDRYARDYWERIPGVYYTGDSAHIDEDGYVWFVGRADEIIKIASHRI
                     GTIEVETAFLKHPAVAEAAVTGRPDQVRGEVISAFIVLKVGHTPSSTLKQELLDTARR
                     ELGPVAVIGELHFISLAPKTRSGKIMRRVLKAIVLGRDPGDVST"
     gene            complement(1874..2665)
                     /locus_tag="VEW91_09430"
     CDS             complement(1874..2665)
                     /locus_tag="VEW91_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM91838.1"
                     /translation="MDYARLFRLDGRVAVVVGAGSGIGQSAARALAAHGAHVVCADLN
                     EAGARETATLIAGAGGRAEPEQVDIAQEESVRRLIATVGSRHPSLDVAVTTPSINVRK
                     PVLAYSAAELDRVLAVNLKGTFYVLREAGRRMAEQGRGSIIAFSSIRAQVVEPGQSMY
                     SATKAGTVQLVRALAAELGPRGVRVNAIAPGVVDTPLTRPITQNPAWYKAYADRSALG
                     RWASPDEIAGAVVFLASDAASYVTGSLLFVDGGWTAVDGRFTPPL"
     gene            complement(2675..>3628)
                     /locus_tag="VEW91_09435"
     CDS             complement(2675..>3628)
                     /locus_tag="VEW91_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HYM91839.1"
                     /translation="WADVPLPNPGPGQVRILVDACGICHTDLHIVEGELRPPALPLVP
                     GHQIVGTVDAVGGGVRTPREGDRVGVGWLAWACGACPYCRDGRENLCERGRFTGFDVP
                     GGYGDATVADARFVYRLPSRYPAPQAAPLLCAGIIGYRSLRLAEVRPGDRVGLFGFGA
                     SAHLAIQVARHWGCEVYVFTRSDAHRQLARTYGAAWVGGMDDTAPEPVDRGIVFAPSG
                     TVVVGALAHLRRGGTLAVNAIHLDRIPEFPYARLYWERTVRSVANATRRDAEEFLALA
                     AQLSLQVSVSEVRPEDANATLGALKRGEIQGAAVLRFSRPS"
BASE COUNT          560 a         1348 c         1220 g          500 t
ORIGIN      
        1 cgtcgagaca tcaccaggat cgcgtcccaa tacgatcgcc ttgaggacgc gccgcatgat
       61 ctttccgctg cgggtcttgg gcgccaggct gatgaagtgg agctccccga tcaccgcgac
      121 cgggccgagc tcccgccggg ccgtatccag cagttcctgc ttgagcgtgc tcgagggcgt
      181 gtggcccacc ttgagaacga tgaacgcgct gatcacctcg ccgcggacct gatcgggccg
      241 gccggtgacc gcggcttccg cgaccgccgg gtgcttgaga aaggcggttt cgacctcgat
      301 cgtgccgatc cggtgggagg cgatcttgat gatctcgtcg gcccggccga cgaaccagac
      361 gtacccatcc tcgtcgatgt gcgcggagtc gccggtgtag tagaccccgg gaatgcgctc
      421 ccagtagtcc cgggcgtagc gatcgggctc gccccacagc gtctggatca gcccggggaa
      481 cggccgcttg atgaccatga tccccttctc gttcgggggg cacggccggc cgtcgggggt
      541 gaccacggcc gcctcgatgc cgggcagggg aatgccagcg gatcccggct tgatcggcag
      601 catcccgagc ccgtacgggt tgccgaacac gggaccgccg gtctccgtct gccacatgtg
      661 gtcgatgacg ggaatgcgat cgcccagcac gcgcgtctgg agccactccc aggccggagg
      721 gttgagcacc tccccggcgc agaagacccg ctcaagcgag gacaggttgc agttgcgggt
      781 gggctcttcg ccgtaccgca tcaggagcct gacgccggtc ggcgaggtga agatgccggt
      841 gaccccaaac tcctcgaccg cccgccagag cacctcggga ccggggtgat caagcgcgcc
      901 ctcgtacgcg accgtcgtgc agccgaacag aagcggtgcg tagacgatgt agctgtgacc
      961 caccacccag ccgatgtcgg aggtcgacca ccagacatcg cccggcgtca tcccgaacag
     1021 ccaccgcccc atgctgtaga tgtagacctg gtatcccccg tgcgtgtgca ccgccagttt
     1081 cggcttggcg gtcgtgcccg aggtcgccag gatgtaggcc ggctcgttgg cctccatcgg
     1141 gaccgatccc ccgctgtgcc cggcgccgca ggcgaggaac tcgggccagg aaagggcctg
     1201 gccgggctgc gcgcctccgg tcccgccccg cgatagcatg atgacccgct ctacagtgtg
     1261 gcccgggccg cggagcgcat cggccacgat gccccagagc ggcacgtcgc gccccttgcg
     1321 gtaggccacg tcggccgtga agacgaggcg cgagccgctc gcgcggatcc ggtcccccag
     1381 ggcgccggcg ccgaaccccg cgaacacgac tgaatggatc gcgccaatcc ggaccgcggc
     1441 gagcatcagg atgatcgcct cggggcacgt gggcatgtag atcgttatcc gatcgccctt
     1501 ccgaatgccc aggccgcgca gggcggccgc cgtccgctcg acctcatggt gcagctgggc
     1561 gtacgtatgc aggcggcgtt ccccccgctc gttgagatag atcagcgcgg cgtgcccccc
     1621 gcggccggcg gcgacgtgcc ggtccaggca gttcacggcg aggttggtct cgccgcccac
     1681 gaaccaccga aacgtcggga agttccactc aaagacccga tcccacggcc gcagccacgg
     1741 cagctgctcg gcggcgcggg cccaaaaccc ctcgacgtcc tcctgcgcct cgcgcagcca
     1801 gcgctggacc acgggattga cgatgtccat gagcctcacc ctcgcgccgg cctcgagacg
     1861 tcgggcgccc ccatcagagc ggcggcgtga accgtccgtc gacggccgtc caccctccgt
     1921 cgacgaagag cagcgacccc gtcacataac tcgcggcatc cgaggccagg aacaccacgg
     1981 ccccggcgat ctcgtccggc gaggcccacc gtccgagcgc actgcggtcg gcgtaggcct
     2041 tgtaccacgc cgggttttgc gtgatcgggc gggtgagcgg ggtgtcgacc acgccgggcg
     2101 cgatcgcgtt gacccgcacg cctcggggcc ccagttcggc ggcgagcgcg cggacgagtt
     2161 gcacggtgcc cgccttggtc gccgagtaca tgctctggcc cggctcgacc acctgcgccc
     2221 ggatcgagga gaacgcgatg atgctgcccc tcccctgctc cgccatccgg cggccagcct
     2281 cgcgcagcac gtagaacgtc cccttcaggt tgaccgccag cacccggtcg agctccgcgg
     2341 cgctgtacgc caggaccggc ttgcgcacgt tgatcgaggg ggtcgtcacc gccacgtcga
     2401 gcgacggatg gcgggaccct acggtcgcga tcaaccgacg gacggactcc tcctgggcga
     2461 tgtccacctg ctccggctcc gcccggccgc ccgcgccagc gatcagggtc gccgtctccc
     2521 tggccccggc ttcgttgaga tcggcgcaga cgacgtgcgc gccgtgcgcc gcgagcgccc
     2581 gggcggccga ctgcccgatc ccggacccgg caccgaccac caccgcgacc ctgccatcga
     2641 gccggaacag ccgagcgtag tccacgtctc tcccctatga cggtcgtgag aatcgcagca
     2701 ccgcggcgcc ctggatctcc ccgcgcttga gggctcccag ggtcgcgttg gcgtcctcgg
     2761 gacggacctc actgaccgag acctgcagtg acagttgcgc ggcaagcgcg agaaactcct
     2821 ccgcgtcccg gcgcgtcgcg ttggccacgc tccggacggt ccgctcccag tacagccggg
     2881 cgtagggaaa ctcggggatc cggtccaggt ggatggcgtt cacggccagc gtgccccccc
     2941 ggcgcaggtg cgcgagcgcg ccaacgacca cggtccccga gggagcaaag acgatcccac
     3001 ggtccaccgg ttccggggcc gtgtcgtcca tgcccccgac ccaagccgcc ccatacgtcc
     3061 gcgcgagctg gcggtgcgca tcgctgcggg tgaagacgta cacctcgcac ccccagtggc
     3121 gggcgacctg gatggcgagg tgggccgagg ccccgaagcc gaacaacccg acccgatcgc
     3181 ccgggcggac ctcggccagc ctgagggagc ggtacccgat gatccccgcg cacagcagcg
     3241 gggctgcctg gggcgccgga taccgggaag gcagccggta gacaaaccta gcgtccgcga
     3301 ccgtcgcgtc gccgtacccg cccggcacat cgaacccggt gaaccgcccc cgctcgcaga
     3361 ggttctcccg cccgtcgcgg cagtaggggc aggcgccaca ggcccaggcg agccacccca
     3421 ccccgacccg gtccccctcc cgcggagtcc tgaccccgcc tccgaccgcg tcgacggtgc
     3481 cgacgatctg gtgccccggc acgagcggga gcgcgggggg ccttagctcc ccctcgacga
     3541 tgtggaggtc ggtatggcag atcccgcatg cgtccaccag aatccggacc tgcccgggcc
     3601 cgggatttgg caggggaacg tccgccca
//
LOCUS       DASQGH010000482         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13595, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000482 DASQGH010000000
VERSION     DASQGH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13595"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..1019)
                     /locus_tag="VEW91_09440"
     CDS             complement(594..1019)
                     /locus_tag="VEW91_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91840.1"
                     /translation="MKVVMLAVAMITLVTLGPGWAAAQQMAPHDDTMMMHGKTLAGVV
                     TRVRFVSCGNTPQTCQGIFEITPAESGAMQPSDAGHMMMQHPVTVIVIPGALLIWQNA
                     PLPLTGLHTGDLVKLEYTELNDMNVVTQLTMTGMAGPHM"
     gene            complement(1329..2207)
                     /locus_tag="VEW91_09445"
     CDS             complement(1329..2207)
                     /locus_tag="VEW91_09445"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYM91841.1"
                     /translation="MRAEDPDHRRDGGQADRDGRRDPPGAEGAVAIAAPPPPILAVDA
                     RRIIRASPARVFAHIGRANYLPRYGAPLWMAAEPVEKRRTAHLIALTGYFVGLPVESV
                     VRVVLHPPHSMEFKQVRGTLRTLSGHCTLRAVEGGTEVHYRLEADLGIPMITDDAARQ
                     FLVQFVERMLDRIKLAAERKAPARRPDRGSAAEPSALEPGLAEPEEDEPEPVMDEGPP
                     AALTEGEAPGAPRVEAADAATAAVDRPPPQAAPPRPAPDARGSSARPGRRRRRRRHHG
                     HGRPPEAPAPGTFPPR"
     gene            complement(2111..>2880)
                     /locus_tag="VEW91_09450"
     CDS             complement(2111..>2880)
                     /locus_tag="VEW91_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYM91842.1"
                     /translation="VAFFADQMDGFAISGLVRSYGNRYYPKPAVVQPVGRRAPITVDW
                     WSYAQSLTSHPVKGMLTGPYTIAEWSFNEHYPTQRELVLELARAIHDEAVDLERAGAR
                     YIQIDEPAIHTRPDEDFDLAVEAMDIVTNGLGVYTISHVCYGDVPRIYPAMLRLAVDQ
                     LDLALKNEDFALLETFKSPRFTKDIGLGVLDAHSHHVETSEEVADGIRRTLRVIPPPQ
                     IYVSPDCGLKTRTTDETVAKLTAMVEGTRRVRKELSR"
BASE COUNT          493 a          930 c          970 g          487 t
ORIGIN      
        1 aatataccac acgaggcgac cgtccctcag cttccccttg tacagcggca aggtgatggt
       61 ggcagtcttg agatccacct gccccgcctt gaggagatgg accggtccca ctaacctcgg
      121 gtccacctgt gatggagtcg ggccaaagta gacgagtggg acatcggcgc cgatggatgg
      181 aggaggaggt gtaacctcct gctctgggcc cccttgggcg cccgatggaa ctggtctgag
      241 cgggatgatc gcaactccca ctgcaagaga catcatggca agccacaaag catggacccg
      301 attgcggata cgcatagaca cttctccttt cgcctcatgg gcgcaattgt ctgagacggg
      361 ataaagaccg agcaccttgc gactgcgaag ataaagagct tcccgggctg taacaaagag
      421 cctcaacgga agactgtaat gatgagacag tggcgatgcg tccaccacgg atggccaggc
      481 cccggccaac ccaggaagcg ctggggaggc cggagctgac caaggggttc gtcagccctg
      541 ctcgagcacg atcgggccaa gccgtcagtg aagacgctta tcggcatccg ggtctacatg
      601 tggggcccgg ccattcccgt catcgtcaac tgggtcacga cgttcatgtc gttgagttcg
      661 gtgtactcca gtttgaccag atcccccgta tggagccccg tcagcggcag cggggcgttc
      721 tgccagatca gcaacgcacc ggggatcacg atcaccgtca ccggatgctg catcatcata
      781 tgaccagcat cggagggctg catcgccccc gactcggccg gcgtaatctc aaagatcccc
      841 tggcaggttt gcggcgtgtt cccgcacgac acgaaccgga cgcgggtcac cacgcccgcc
      901 aacgtcttgc catgcatcat cattgtgtcg tcgtgcgggg ccatttgttg ggccgccgcc
      961 cacccgggac ctagggtgac cagggtgatc atcgctaccg cgagcatgac taccttcatc
     1021 tccacacctc cttgatcggt aaggacacag cttcaccgta tcaaggtcgt gtttaggttt
     1081 gataaagctc cccataagga aaggctgcct gtaaagacgc gccacgtttc gagcggccgt
     1141 tgtgcgctaa ggggagggat atacggggag ggaagtggcg gccgagatca tgcgcgcgcc
     1201 gcgtgaggtc tcaatggcgg cgggttgagc ctgggggcaa gattgggttg gtgtaggtag
     1261 tgggtcaaac gaagcgctgc ttcgcggact cgaacccgga gatgcgatct cgatcgcggg
     1321 agaacgagtc agcggggcgg gaaggttccc ggggcgggcg cctccggcgg ccggccgtgc
     1381 ccgtggtgac ggcgcctccg gcggcgacgg cctggacgtg cagacgagcc gcgcgcatcc
     1441 ggggccggcc gtggtggggc cgcctggggt ggcggacgat ccaccgcggc agtggcggcg
     1501 tccgctgcct ccacgcgtgg cgcccccggg gcctcgccct ccgtgagggc cgcggggggg
     1561 ccctcatcca tcacgggttc gggctcgtcc tcctccggct ctgcaagccc gggttcgagc
     1621 gcggagggct ccgctgcgct cccccggtcg ggccgccggg cgggcgcttt gcgctccgcc
     1681 gccagcttga tccggtcaag catgcgctcc acgaactgga cgagaaactg gcgggccgcg
     1741 tcatcggtga tcatcgggat gccaagatcg gcctcgaggc ggtagtgcac ctccgtcccc
     1801 ccctcgacgg cccgcagggt gcagtgcccg ctcagcgtcc gcagggtccc gcgaacctgt
     1861 ttgaactcca tcgagtgcgg gggatggagc acaacgcgga ccaccgactc caccggcagc
     1921 ccgacgaagt agcccgtgag cgcgatcagg tgcgcggtcc ggcgtttctc caccggctcc
     1981 gcggccatcc acagaggcgc tccgtaccgg gggaggtaat tggcacggcc gatgtgcgcg
     2041 aagacgcggg ccggcgaggc gcgaatgatc cggcgcgcgt cgaccgccag gatgggcggc
     2101 ggaggcgcgg ctatcgcgac agctccttcc gcacccggcg ggtcccttct accatcgcgg
     2161 tcagcttggc caccgtctcg tcggtggtcc gggtcttcag cccgcagtcc ggcgagacat
     2221 agatctgggg gggtgggatg acccggagcg tccggcggat cccgtccgcg acctcttccg
     2281 atgtctccac gtggtggctg tgggcatcga gcacacccag gccgatgtcc ttggtgaacc
     2341 gcggggactt gaacgtctcg aggagcgcaa agtcctcgtt cttgagcgcg aggtcgagtt
     2401 ggtcgaccgc cagccgcagc atcgccgggt agatccgggg cacatcgccg tagcagacgt
     2461 gcgagatggt gtacaccccc agcccgttgg tgacgatgtc catggcttcg accgccaggt
     2521 cgaagtcctc atccggcctc gtgtggatcg ccggctcgtc gatctggatg taccgggccc
     2581 ccgcccgctc cagatccacg gcctcgtcgt ggatcgccct ggcgagctcc agcaccagtt
     2641 cccgctgcgt cgggtagtgc tcgttgaacg accactccgc gatggtgtat ggcccggtca
     2701 gcatcccctt caccgggtgg gaggtcaggc tctgcgcgta ggaccaccag tccacggtga
     2761 tgggcgcccg ccgtccaacc ggctgcacga ccgccggctt gggatagtag cgattgccgt
     2821 acgagcgcac caggccggag atcgcgaacc cgtccatctg gtcggcgaag aacgccacca
//
LOCUS       DASQGH010000483         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36125, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000483 DASQGH010000000
VERSION     DASQGH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36125"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VEW91_09455"
     CDS             complement(<1..1395)
                     /locus_tag="VEW91_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYM91843.1"
                     /translation="MTPAGRAVLGLTLPQVLEARAAGPESRRIALRYKDRGIWQEWTW
                     QDYLAEARAFGLGLVALGLNPGEKVAILSDNRPEWLYAEMGTLAVGGIAVGVYQDAIA
                     REIGYIVDHADATCVVAEDQEQLDKILEVRGDLPKLRRIITVDPKGMHRVRDPNVMRF
                     PEVCELGRRYDRDHPGAFGELLRSSAPEDIANIVYTSGTTGSPKGAMLTHRNLINMAV
                     TFAEVDPVRPEDQLFSYLPMPWIGEIAMSIATSLVFGITVNFPEEVETTHDDGREIGP
                     HILFGPPRLWEALTSEVQVKIMDTTPLARAVYRLAMRWGYRVADRRLRGKRLTLIDRG
                     LYGLGYLLAFRAVLDDMAMLRIRHAYTGGAALGPDTFRFLRALGLNLKQLYGQTEIAG
                     YSCVHRDGDIRFETVGQPTPGVEVRISPEGEILERSESVFLGYYKDPQATREALEDGW
                     LHTGDAGSLTEDGHL"
     gene            complement(1392..2180)
                     /locus_tag="VEW91_09460"
     CDS             complement(1392..2180)
                     /locus_tag="VEW91_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91844.1"
                     /translation="MAARAADSLLAAESVSLGFGGLTVLNNVSLAVRPGERVAVIGPN
                     GAGKTSLLNCINGFYHPDSGHIRYRGRDITRAAPYEIARLGMGRTFQNIELFTGATVL
                     DNLLLARHRHIRYGLLAAALYYGRAQAEETTHRMRVEEVIDFLELEPIRHAIVGGLPY
                     GRRKRVELGRALATDPELLLLDEPMAGMTIEEKQDMVRFIVDANESLGITVVLIEHDM
                     GVVMDVAQQVFVLDFGALIGEGTPAEIRANPHVVRAYLGDEVAS"
     gene            2345..2821
                     /locus_tag="VEW91_09465"
     CDS             2345..2821
                     /locus_tag="VEW91_09465"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HYM91845.1"
                     /translation="MWQLPKAVSLVAGCGEGSTELNAFDRALQDAGVADINFLRVTSI
                     MPPGARVIELPPYPAGLLLPAVYARIASTRPGDRICAAIAVGVSRESYGVIMEHTAHT
                     GRAEAERVVRGMVEEGLRIRGRAADEVITVASEHTVTRAGCAVALALFWPTPTGVP"
BASE COUNT          436 a         1062 c         1006 g          454 t
ORIGIN      
        1 gaggtgaccg tcctcggtga gggagccggc gtcgccggtg tggagccacc cgtcctcgag
       61 cgcctctcgg gtggcctggg ggtccttgta gtagccgaga aagacgctct cgctgcgctc
      121 gaggatctcg ccctcgggcg agatccggac ctcgacgccg ggggtgggtt ggcccacggt
      181 ctcgaaccgg atgtcgccgt cccggtggac gcagctgtac ccggcgatct cggtctgccc
      241 gtacaactgc ttgaggttga gcccgagcgc ccggaggaac cggaaggtgt ccggccccag
      301 ggccgccccc ccggtgtagg cgtggcggat ccggagcatc gccatatcgt cgaggaccgc
      361 ccgaaacgcc aggagatagc cgagcccgta gagcccccga tcgatcagcg tcagccgctt
      421 cccccggagg cgccggtcgg ccacccgata cccccagcgc atcgccagac ggtagacggc
      481 ccgcgcgagc ggcgtcgtgt ccatgatctt gacctggacc tcgctggtaa gcgcctccca
      541 caggcggggc ggcccgaaga ggatatgggg cccgatctcc cggccgtcgt cgtgggtggt
      601 ctccacctcc tcgggaaagt tcaccgtgat cccgaacacg agggaggtgg cgatcgacat
      661 cgcgatctcg ccgatccacg gcatcgggag gtacgagaac agctggtcct ccggccggac
      721 cggatcgacc tcggcaaagg tcaccgccat gttgatgagg ttccgatggg tcagcatcgc
      781 ccccttggga gaccccgtcg tcccggacgt gtagacgatg ttggcgatgt cctcgggcgc
      841 gctactccgc aggagctccc cgaaggcgcc ggggtggtcg cggtcgtagc ggcggccgag
      901 ctcgcacacc tcgggaaagc gcattacgtt gggatcccgg acccggtgca tgcctttcgg
      961 atccacggtg atgatccggc gcagcttcgg gaggtcgccc cggacctcca ggatcttgtc
     1021 cagctgctcc tggtcctccg ccacgacgca ggtcgcgtcc gcgtggtcga cgatgtagcc
     1081 gatctcccgg gcgatcgcgt cctggtagac gccgaccgcg atccccccca ccgcgagcgt
     1141 ccccatctcg gcgtagagcc actccgggcg gttgtcgctg aggatcgcca ccttttcgcc
     1201 ggggttgagg cccagcgcca ccaaccccag cccgaacgcg cgcgcctctg ccaggtagtc
     1261 ctgccaggtc cactcctgcc agatcccgcg gtccttgtag cggagcgcga tgcggcggct
     1321 ctccggtccc gccgcgcggg cctcgagcac ctgcgggagg gtcagcccca gcacggcgcg
     1381 tcccgccggg gtcatgaggc gacctcgtcg cccaggtagg cccgcaccac gtgggggttt
     1441 gcgcggatct ccgcgggggt gccctctccg atcagcgcgc cgaagtccag gacgaacacc
     1501 tgctgggcca cgtccatgac gacccccatg tcgtgttcga tcagcaccac cgtgatcccc
     1561 agcgattcgt tggcatcgac gatgaaccgg accatatcct gtttctcctc gatggtcatc
     1621 ccggccatcg gttcgtcgag gagcaacagc tccgggtcgg tcgccagggc ccgccccaac
     1681 tcgacccgct tgcgacgccc gtagggcagc ccccccacga tcgcgtgccg gatcggctcg
     1741 agctcgagaa agtcgatgac ctcctcgacc cgcatccggt gagtggtctc ctccgcctgc
     1801 gcgcggccgt agtacagcgc ggccgccagc agtccgtacc ggatgtgccg gtggcgcgcg
     1861 agcaggaggt tgtccagcac ggtcgccccg gtgaagagct cgatgttctg gaaggtgcgg
     1921 cccatcccga gccgggcgat ctcgtagggc gcggcccgcg tgatgtcccg accgcggtag
     1981 cggatgtggc cgctgtcggg atggtagaag ccgttgatgc agttgagcag gctcgtcttg
     2041 cccgccccgt tggggccgat cacggcgacg cgctcgcccg gccggacggc gagggacacg
     2101 ttgttgagca cggtgaggcc gccgaagccc aaagacacgg attccgccgc gagcagagag
     2161 tccgccgccc gggccgccac gcccgcaacc cctagaccca acgcccccaa cgtgccgggg
     2221 atcttcgacc tggcgaaggg cgttcccctc ctgcccggcc ctcgcgcgcg atcgccgggc
     2281 gatccgctcc gcgcgaccgc gcgcccacaa gggcttccat cggtctggtg gaatatctag
     2341 cgccatgtgg cagttgccga aggccgtctc gctcgtcgcc ggatgtggcg aaggcagcac
     2401 cgagctcaat gccttcgacc gggcgctcca ggacgccggt gtcgccgaca tcaacttcct
     2461 ccgcgtcacg agcatcatgc ccccgggagc ccgggtgatc gagctccccc cctacccggc
     2521 ggggctcctc ctccccgccg tctacgcgcg catcgccagc acccggccgg gggaccggat
     2581 ctgcgccgcg atcgccgtcg gggtctcccg cgagagttac ggggtgatca tggagcacac
     2641 ggcccatacg gggcgggccg aggcggagcg cgtcgtccgg ggcatggtcg aagaggggct
     2701 ccggatccgc gggcgcgcgg ccgacgaggt gatcaccgtc gcatccgagc acacggtcac
     2761 gcgggccgga tgcgcggtgg ccctggcgct gttctggccg acccccaccg gcgttccgtg
     2821 atgaagggcg cctggatcat cgatcagggt gggggggatg tcacctccgc ccatcgggtg
     2881 acccggagac tgtacgagga gcgcaccccc caccagcacc tcgagatcta cgacagcccg
     2941 ctgttcggcc ggatgctc
//
LOCUS       DASQGH010000484         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_52965, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000484 DASQGH010000000
VERSION     DASQGH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52965"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VEW91_09470"
     CDS             complement(<1..417)
                     /locus_tag="VEW91_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYM91846.1"
                     /translation="MAETESHPTGNTFAIVNQKGGVGKTTTAVNLSAALACLGHRVLL
                     IDLDPQGNSTSGVGVAKSGVRVSMYDILIGGDPMFHVIQPTGIPRLDLAPSEVRLAGA
                     EVELVPAIARETKLRNAIRAIREIYDVLLIDCPPSLG"
     gene            complement(600..1316)
                     /gene="rsmG"
                     /locus_tag="VEW91_09475"
     CDS             complement(600..1316)
                     /gene="rsmG"
                     /locus_tag="VEW91_09475"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HYM91847.1"
                     /translation="MSVGRILQSGGAALGVNLSSAQRSLLERYIEVALSWRRRLNLTG
                     AETPEEAARVLVLDALACIAHLPHRGTIVDLGSGAGTPGVPIAVACPEARVFFVEASR
                     KKAGFLEVILRELALANADVLHARAEALGRDPAHRERHDAVTARALAALPVLVELALP
                     LIRVGGVGVFPKGPTAEAEVARAAHALGLLGGAAEVRVSPRPPGSRLVIVRKVASTPA
                     AYPRRPGVPARRPLMAGEPL"
     gene            complement(1324..>2066)
                     /gene="jag"
                     /locus_tag="VEW91_09480"
     CDS             complement(1324..>2066)
                     /gene="jag"
                     /locus_tag="VEW91_09480"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HYM91848.1"
                     /translation="EGSGRTVEEAIRDALRVLGVGREEVDLMVLDEGSRGVLGLGTRS
                     ARVRLTLLSEMEEEGGATTLEPPALVQMAPGEAGELAQLAQTITSSLIEAMGFHATVT
                     AREEAAQIHVSVTGPDLAPLIGRRGQALEALDLLVNLITAHRQGHRVPVVVDVERYRE
                     RRVETLQEVARRFAERARRTGRPVALKPMSAAERRIVHTTLAEDGGVSTHSEGEDPDR
                     HVVITPGRTPSPGVLNSAGRHTPNNTRS"
BASE COUNT          318 a          734 c          715 g          299 t
ORIGIN      
        1 ccccagggac ggagggcagt cgatgagcag cacgtcgtat atctcccgga tggcccggat
       61 cgcgttcctg agtttggtct cccgggcgat cgccggcacc agctcgactt cggccccggc
      121 cagcctgacc tccgaggggg ccagatcgag ccgggggatc cccgtcggct gaatgacgtg
      181 gaacattgga tccccgccga tcaggatatc gtacatcgag acccggacgc cggacttggc
      241 gacgccgacc ccgctggtag agttgccctg ggggtccaga tcgatgagga ggacacggtg
      301 ccctagacag gccagcgcgg cgctgaggtt gacggcggtg gtcgtcttgc ccacaccgcc
      361 cttttgattg acgatcgcga acgtgttccc cgttgggtgc gactccgttt ctgcgattta
      421 ggaccgacgt cccacgacga ccgacgatgg gggcactcgc aatcgagcac cagttccccg
      481 ttgtgcggga aaactcctcc ccgagggggg gacgcagcgg gcggggggtt caataggcgg
      541 gaccggcctc aagccctgca atactgcgcg agatggctag ccatcaagac atcttactat
      601 cagaggggct ccccggccat cagcgggcgc cgggcgggca cccccgggcg gcggggatat
      661 gctgcgggcg tggaggcgac ttttcgcacg atcacgaggc gcgatcccgg cggccggggg
      721 gagacccgca cctccgcggc gccccccagc agaccaagcg cgtgcgcggc gcgagccacc
      781 tccgcctccg cggtgggccc tttcgggaac accccgacac ctccgaccct gatcaggggg
      841 agcgcgagct cgaccagcac cgggagcgca gcgagcgcgc gggcggtgac ggcatcgtgc
      901 cgctcgcggt gcgccgggtc gcgccccagc gcctccgccc gcgcgtggag cacgtccgca
      961 ttggcgaggg cgagctcacg gaggatcacc tccagaaacc cggccttctt gcgggacgcc
     1021 tccacgaaga aaacccgcgc ctccggacac gccaccgcga tggggacccc gggcgtaccc
     1081 gccccgctcc caagatccac gatcgtcccc cgatgcggca gatgcgcgat gcaggcgagg
     1141 gcatccagga ccagcacacg tgcggcctcc tccggggtct cggccccggt gaggttcagg
     1201 cgccggcgcc aggacagcgc aacctcgatg tagcgctcga gcaggctgcg ctgggcggag
     1261 gagaggttga cgccgagggc tgccccgccg gactggagga tccgcccgac ggacaccgcg
     1321 accctatgac cgggtgttgt tgggggtgtg gcgccccgcc gagttgagca cccccgggga
     1381 cggggtcctc ccgggagtga tcaccacgtg gcggtccggg tcctcgcctt cgctgtgcgt
     1441 agatacccct ccgtcctcgg cgagcgtggt gtggacgatc cggcgttcgg ccgcggacat
     1501 cggcttcagg gccacgggcc gccccgtccg gcgggcgcgt tcggcaaacc ggcgggccac
     1561 ttcctgcagc gtttcgaccc gccgctcgcg gtaccgctcg acatctacca cgaccgggac
     1621 ccggtgccct tggcggtggg ccgtgatcaa attgacaaga aggtctagcg cctccagagc
     1681 ctggccgcgc cggccgatca gcggggcgag atcggggccg gtgacggaga cgtggatctg
     1741 ggccgcttct tcccgcgcgg tgaccgtggc atggaagccc atggcctcga taagcgagga
     1801 cgtgatcgtt tgcgccagct gggccagctc acccgcctcc ccgggggcca tctgcaccag
     1861 ggcggggggc tctagggtgg tggcaccgcc ctcttcctcc atctccgaaa gcagggtgag
     1921 ccggacccgc gcacttcgcg tgcccaatcc cagcactccg cggctcccct cgtccagcac
     1981 catgagatcg acctcctcgc ggccgactcc cagaacgcgc aaggcatcgc ggatcgcctc
     2041 ttcgaccgtc cgtccggacc cttcag
//
LOCUS       DASQGH010000485         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27524, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000485 DASQGH010000000
VERSION     DASQGH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27524"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VEW91_09485"
     CDS             <1..731
                     /locus_tag="VEW91_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HYM91849.1"
                     /translation="SIEDTIRVVCRYRPAAIVIRHYQEGAAERAAGVAADPRTRRPVP
                     VFNAGDGPGQHPTQALLDAYTIWRVRGTLEGTTVAVAGDLLNGRTARSLCYLLAKYPG
                     VSFRLIAPKPVQMKEDLLAYLRRHKVPFAQTEDLAAGVRGVDVLYVTRIQKERFEDGA
                     EAQYERVRDAFSVTSDVLTELPAHAVVMHPLPRVEGARSELPTSVDADPRVIIFDQTE
                     AGMYIRMALLKLTCAVDRVAGRAR"
     gene            695..1930
                     /locus_tag="VEW91_09490"
     CDS             695..1930
                     /locus_tag="VEW91_09490"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYM91850.1"
                     /translation="MRRGPRRRPRPLGAGARCAPRGLPIGLGAAAAVLLWPARAYAHH
                     LSGGPPSGPEIAALATGLAAALAGWLVIAQSRRSPAHPAVRRWRVRSGYALFLVGLLI
                     FLGGPELADYVSSRTPAPGGAARVGPPAPGSVTLRWADWTWDPLIVVGLGATVLAWWW
                     VARRFRPRRWQPVFFWAGMLGLVVALLSPIDAGSEYLFTIHMVQHMLLMLVVPPLLAL
                     AVPPALLGRLYQQPGIARVLRAVWSPLPALVLFNGVLIFWHLPFAYDATLDWAWVHAV
                     EHLSFLLAGLVFWGVIASPAPKLVRASFGLRLGLVVAADLVNFLVGFALAFAGRPFYR
                     HYTQVPRLWGLSPLDDLHLGGGVMWVMGQMMYAIPVLILLSVLLRREDAPGSSPHGAG
                     HLSGRPGESPVGSMPSPHP"
     gene            2002..2760
                     /locus_tag="VEW91_09495"
     CDS             2002..2760
                     /locus_tag="VEW91_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91851.1"
                     /translation="MAEPLLVLRGVGKSFGGVVAVRGVSTALAAGELRGLIGPNGSGK
                     TTLLNLIGGQLLPDAGEIHLDGRPIQGLAPDAIAVLGVARTFQLPRVFRHMTVLDNLL
                     LPFCADHQRTRWAEAHEAARRLLEFVGLDSLARAEARVLSGGQGMLLQLARCLIQRPL
                     RVCMLDEPFAGVHPAIKDRMVAAILEINRREGVTFLIVSHEMATLRKLAGRVSVMHNG
                     EWIAEGTLDEVAGDARVIEAYLGARRALAPAGGA"
     gene            2781..>2996
                     /locus_tag="VEW91_09500"
     CDS             2781..>2996
                     /locus_tag="VEW91_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91852.1"
                     /translation="MLRVDRVVAGYHPGIDILNDLTVRAAPGRITAVIGPNGAGKSTL
                     LRVVFGLVPPRAGRVLFGEREIQGLAAD"
BASE COUNT          356 a         1048 c         1086 g          506 t
ORIGIN      
        1 agtcgatcga ggacacgatc cgggtggtgt gccggtaccg gccagcggcg atcgtcatcc
       61 gacactacca ggaaggcgcc gcggaacgcg cggccggcgt ggcggcggat ccgcgcacca
      121 ggcgcccggt gcccgtgttc aacgcgggag atggccccgg gcagcatccc acccaggcgc
      181 tgctcgatgc gtacacgatc tggcgcgtcc gcggcaccct ggaggggacg accgtcgccg
      241 tggcgggcga tctcctgaac gggcggaccg cccggtcgct gtgctacctg ctggccaagt
      301 acccgggggt gtccttccgg ctgatcgcgc ccaagccggt gcagatgaag gaggacctgc
      361 tggcctacct acgccgccac aaagttccct ttgcccagac cgaggatctt gcggcggggg
      421 tccgcggcgt ggacgtgctc tacgtgaccc ggatccagaa ggagcggttc gaggacggcg
      481 ctgaggccca gtacgaacgc gtccgggacg cgttttccgt gacctcggac gtgctcacag
      541 aactcccggc ccacgcggtc gtgatgcatc cgctcccgcg ggtcgaaggc gcccgcagcg
      601 agcttccgac atcggtcgac gcggaccccc gggtgatcat ctttgatcag acagaggccg
      661 ggatgtacat ccggatggcc ctgctgaaac tcacgtgcgc cgtggaccgc gtcgccggcc
      721 gcgcccgcta ggcgccggcg ctcggtgcgc tccccgcggg ctcccgatcg ggctgggggc
      781 cgcggctgcc gtcctcctgt ggccggcgcg ggcgtacgcg caccacctgt ctgggggtcc
      841 accctcgggc cccgagatcg ccgcgctcgc caccggcctg gctgccgcgc tcgcggggtg
      901 gctcgtcatc gcgcagagcc gaaggagccc tgcccacccg gccgtgcggc ggtggagggt
      961 caggagcggc tacgcgttgt ttctcgtcgg cctgctcatc tttctcggcg ggcccgagct
     1021 cgccgattac gtgtcctccc gcacccctgc cccgggcggc gccgccaggg tcggtccacc
     1081 ggcccccggg agcgtgaccc tccggtgggc cgattggacc tgggatccgt tgatcgtcgt
     1141 ggggcttggg gcgacggtcc tggcctggtg gtgggtcgcg cgccggtttc gtccgcggcg
     1201 ctggcagccg gtcttcttct gggccggcat gctcggcttg gtcgtggccc tgctctcccc
     1261 gatcgacgca gggtccgaat acctctttac gatccacatg gtgcaacaca tgctcctgat
     1321 gcttgtggtc ccgcccctgc tcgcgctcgc ggttccgccg gcgctgctcg ggaggctgta
     1381 ccagcagccc gggatcgccc gcgtgctgcg cgccgtgtgg tcgccgctgc ccgcgctcgt
     1441 cctgttcaac ggggtgctga tcttctggca cctgccgttt gcctacgacg ccacgctcga
     1501 ctgggcctgg gtccacgcgg tggagcacct gagctttctg ctcgccggat tggtcttctg
     1561 gggggtcatc gcgtcccccg ctccgaagct ggttcgcgcc tcgttcgggc tgcggctcgg
     1621 cctggtcgtc gccgccgatc tcgtcaactt cctggtgggg ttcgcgctgg cgtttgccgg
     1681 ccggccgttc taccggcact acacccaggt cccccgcctg tggggcctga gcccgctcga
     1741 cgacctccac ttgggcgggg gggtgatgtg ggtgatgggc cagatgatgt acgcgatccc
     1801 ggtgctgatc ctgctcagcg tattgctgcg ccgggaggat gcgcccggat cgtccccgca
     1861 cggtgccggc catctttcgg ggcggccggg ggagtcgccg gtcgggtcaa tgccgtcgcc
     1921 ccatccctga acggtcggac accgcatcgg cccgagcccc gaggcacagg gcagcgggat
     1981 gcccccgccg aaccctccgg cgtggccgag cctctgctgg tcctccgggg tgtcgggaag
     2041 tcgttcgggg gcgtcgtcgc ggtgcggggt gtcagcaccg cgctcgccgc gggcgagctc
     2101 cgcgggctga tcggtcccaa tggatccggg aagaccaccc tgctgaacct gatcgggggg
     2161 cagctgctgc cggatgccgg cgagatccac ctcgacgggc ggccgattca ggggctggct
     2221 cccgatgcga tcgccgtgct cggggtggcg cggacgtttc aactcccgcg cgtgttccgt
     2281 cacatgaccg tcctggacaa cctgctcctc ccgttctgcg ccgatcacca gcgcacccga
     2341 tgggccgaag cgcacgaggc cgcccgccgg ctgctggagt tcgtggggtt ggactcgctg
     2401 gcccgtgcag aggcccgggt gctgtccggc ggacagggga tgttgctcca gctcgcccgc
     2461 tgcctcatcc agcggccgct gcgggtgtgc atgctcgacg agccgtttgc cggcgtgcat
     2521 cccgcgatca aggaccggat ggtcgcggcg atcctcgaga tcaaccggcg ggagggggtc
     2581 acattcctca tcgtcagcca cgagatggcc acgttgcgaa aattggccgg ccgggtgtcg
     2641 gtgatgcaca acggggagtg gatcgccgaa gggaccctgg acgaggttgc cggcgatgcc
     2701 cgggtgatcg aggcctacct cggagcccgc cgcgcgctcg ccccggcggg cggggcgtag
     2761 cccgggagag gcgccggcgc atgctgcggg tcgatcgcgt ggtcgccggg tatcacccgg
     2821 ggatcgatat cctcaacgat ctcaccgtac gggcagcccc gggccggatt accgcggtca
     2881 tcggccccaa cggggccggg aagtcgaccc tcctgcgggt ggtgttcggc ctggtcccgc
     2941 cccgtgcggg ccgcgtgctg ttcggggagc gcgagatcca ggggcttgcg gccgac
//
LOCUS       DASQGH010000486         7187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30567, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000486 DASQGH010000000
VERSION     DASQGH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30567"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..963
                     /locus_tag="VEW91_09505"
     CDS             115..963
                     /locus_tag="VEW91_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HYM91853.1"
                     /translation="MARSVLIVGVGGQGAVLAGNILGEVAMGAGFQVKKSEVHGMSKR
                     GGIVFSHVRYGPEVHSPLIGAGEADALVAFEWAEGLRWLPYLRPDGILVVDTAQILPP
                     AAQRDHRAWARAYPELDPTLLEGHRGPVLAADARGLAQHLGSPHAANTVLLGILSLRL
                     EFPPEAWETAIRRQVPPRTVEMNLRAFREGRDLKPAALPTGTGRWAPPAGGGAYRIEI
                     TSPWCKACDICVRVCPEACLRLGESGTVEVAASDACTGCRLCEWLCPDFAIAVHPAAP
                     VRGGTS"
     gene            960..2108
                     /locus_tag="VEW91_09510"
     CDS             960..2108
                     /locus_tag="VEW91_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYM91854.1"
                     /translation="MSAARLVQGNAACAAGAVAAGCRFYAGYPITPSSEIAEEMARRL
                     PALGGVFVQMEDEIASLAAVIGASLGGMRAMTATSGPGFSLMQEHIGFAAMAEVPCVI
                     VNVMRGGPSTGLPTSPAQGDVMQARWGTHGDVPSLVLAPASVAEVYDLTVAAFALSER
                     FRTPVIVLYDEVIGHMREEVALRDLPEGGVPGRRRPAVPPSEYRPYEPDADGVPPMAD
                     FGSGYRFHVTGLAHDARGYPTQDPATIAAHQRRRLEKITGFADLVAPTRALYLDDAEV
                     AVVAVGIAARAAQAAVDEARGRGIRAGLLRPAVLWPFPDEAVREVASSVRAIVVAEMN
                     LGQIVREVERAAGGRATVVPCCRADGTALEPEQVLASLEALMPAGRRA"
     gene            2108..2950
                     /locus_tag="VEW91_09515"
     CDS             2108..2950
                     /locus_tag="VEW91_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYM91855.1"
                     /translation="MVDIHRYLRLEQMPHVLCPGCSHGIVAGALLRAIDRLALPQDRV
                     AVVAGIGCSSRLPGSLDFCTLHGTHGRAPAFATGLKLARPDLHVVLITGDGDGLAIGG
                     NHLIHAARRNIELTCLLFNNEVYGMTGGQLAPTTPEGGVATTAPFGNVEPPFEPCRLM
                     AAAGATFVARGIAYEPTVLEDMIARGLRHKGFAFIEVLTDCPEYYGRYNQLGSPTAML
                     FAQKRAVAAIDAQLDAGHATILHAAPARRLAPRVETGVLVETHRPEFAERTRALMAVA
                     QGRR"
     gene            2947..3510
                     /locus_tag="VEW91_09520"
     CDS             2947..3510
                     /locus_tag="VEW91_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HYM91856.1"
                     /translation="MTLAVRLGGAGGQGIVLAGLILAEAAALAGRRVVCTQAYGPESR
                     GGASRSDVIISDQPIVFPMARRLDVLVALTQEACDRSWGALAPNGLAIIDAGRVTLPP
                     RGPAECHALPVTGTARLVTGSTRSANIVALGAVCGLTGVVPRPALEEAVARRVPSASR
                     GLNLAALGAGERLGVEARAAVAAGGGR"
     gene            3513..4223
                     /locus_tag="VEW91_09525"
     CDS             3513..4223
                     /locus_tag="VEW91_09525"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYM91857.1"
                     /translation="MGPDRGGRAAGAILEDAGSPAGGNTLAVPAPASLGTGRDSNTRA
                     RILRAAAALFHRQGFDRTTMQEIAAASGLTKGSIYHHIRSKQALLYEILQHTLDRSLP
                     DLERIAAARLPAAERLRRAVRLHILTLVSDRDNVSCFIEEGRSLAPEYQAAHLAARDR
                     YEALFRRIVEDGIGSGEFAPTDVRLAGLAVLGMVNWMVRWYRPEGPRPAEEIAARFGD
                     YAVGALAGARGEHDSPRA"
     gene            4276..5418
                     /locus_tag="VEW91_09530"
     CDS             4276..5418
                     /locus_tag="VEW91_09530"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYM91858.1"
                     /translation="MHLADLGAAVVKVEPPGGEWGRTLGPPFLDGVAAAFVGMNRNKR
                     SLVVDLKQAAGREVVARLAEASDVLVESFRPGVMARLQLDYDTLAPRCPRLIYCAISA
                     FGQDGPWRDKPGVDGIVQGASGLMSVTGEPGGAPVKVGVPAADTVAGFLAAQGILAAL
                     FGRERTGRGQRVDLALLDALLAFQAVPLAMFLSSGKDPGRSGSAAPYAAPNEAFPTED
                     GFIMVAAYTPGRWTKLCEVLGWAKLATDPRFDSNEKRVRNRAALRGILTPIFQTRTSA
                     AWVSTLEEADILCGPIATYPEVVVHPQVVHNRMVVALDHPQLGRTAVVGSPLRFSATP
                     TAEAAPAPLAGEHSEAVLLRAGFSGDEIATLMARRIVQGPAVAVGT"
     gene            5455..6243
                     /locus_tag="VEW91_09535"
     CDS             5455..6243
                     /locus_tag="VEW91_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM91859.1"
                     /translation="MGETVLFEQNAAIGMLTLNRPEALNALTAEMLDRIGEFLLKAEA
                     SGDVRALILTAAGEKAFCVGADLKSRAQEYESDGAGPDPLGDVVRRVFGQIEALRLPV
                     IAAINGYALGGGLELALACDLRVAAEGARLGLPEAKVGSMPGAGGTQRLARLIGPARA
                     KELMFTGEFVDAGEAHRIGLVNRVFPAAGLMEGARALAQTIASRAPLSVHAIKAAVNL
                     SHDVSLDAGLAFERTAHAVLRASRDRREGIQAFVEKREPHFVGR"
     gene            6264..7010
                     /locus_tag="VEW91_09540"
     CDS             6264..7010
                     /locus_tag="VEW91_09540"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYM91860.1"
                     /translation="MGVRGRVAVVTGAGQGIGEGIAVHLHAAGARVVVNDLVPERVQR
                     VVAEMNDGGPEALGVAADVSRAEGAVALIRQARKAFGRVDILVNNVGIARDRYLTKMS
                     EEDWDEVLRVNLKSYYLCCHEAAPHMMEQQHGRIINISSRAWLGGPGQANYAASKGGV
                     VSLTRTLALELAKFGITANAIAPALVDTPLFRSLKDEIQERLTKTVPVGRIATPADIA
                     NAALFFASDESSYVTGQLLYVCGGRSVGAY"
BASE COUNT         1002 a         2462 c         2683 g         1040 t
ORIGIN      
        1 acgcggcgct gtgctctggg tgcgcgatct gcccgcaggt ctgcccgact ggcgcgatcg
       61 tgtcaattcc tgcggcgcgc aacggcgggg tggcgcgcgc ggaaggtcat gccgatggcg
      121 cgtagcgtcc tcatcgtcgg cgtcggcggg cagggcgcgg tgctcgccgg caacatcctc
      181 ggcgaggtgg cgatgggcgc cgggttccag gtcaagaaga gcgaggtcca cggcatgtcc
      241 aagcggggcg gcatcgtctt cagccacgtc cgctacggtc cggaggtgca cagcccgctg
      301 atcggcgcgg gcgaggccga tgccctggtg gcgttcgagt gggccgaggg gctgcggtgg
      361 ctcccctacc tccggccgga tggcatcctg gtggttgata cggcgcagat ccttcccccg
      421 gccgcacagc gcgatcaccg ggcgtgggcg cgggcgtacc ccgaactcga tccgacgctg
      481 ctggagggcc atcggggacc ggtgctggcc gccgatgcgc gcgggctggc gcagcacctg
      541 ggctcaccgc acgcggccaa cacggtcctg ctcggcatcc tctccctgag gctggagttc
      601 ccccccgaag cctgggaaac ggcgatccga cggcaggtgc ctccgcgtac ggtggagatg
      661 aacctgcgcg cgttccgaga gggccgggat ctgaagcccg ctgcgctccc caccggcacc
      721 ggccggtggg ctcccccggc cggcggcggc gcgtaccgga tcgagatcac ttctccgtgg
      781 tgcaaggcct gcgacatctg cgtgcgcgtc tgccccgagg catgcctgcg gctgggggaa
      841 agcgggacgg tcgaggtggc cgcatccgat gcgtgcaccg gatgccgcct ctgcgagtgg
      901 ctctgcccgg acttcgcgat cgccgtgcac ccggcggccc ccgttcgcgg aggaacgtcg
      961 tgagcgcggc ccggctggtc cagggcaacg ccgcgtgcgc ggccggcgcg gtcgcggcgg
     1021 ggtgccgctt ctatgccggc tacccgatca cgccgtcctc ggagatcgcc gaggagatgg
     1081 cgcggcggct gcccgcgctg ggcggcgtct tcgtgcagat ggaggacgag atcgcctcgc
     1141 tcgccgccgt gatcggcgcc tcgctgggcg ggatgcgcgc catgaccgcc accagcggtc
     1201 ccgggttctc cctcatgcag gagcacatcg gcttcgcggc gatggccgag gtcccgtgcg
     1261 tgatcgtcaa cgtgatgcgc ggcggaccga gcacggggct gcccacctcg ccggcgcagg
     1321 gagacgtgat gcaggcccgg tgggggacgc acggcgatgt ccccagcctc gtccttgcgc
     1381 cggcctcggt ggccgaagtg tacgacctga ccgtggcggc attcgccctt tctgagcgat
     1441 tccggacgcc ggtcatcgtg ctctacgacg aagtgatcgg acacatgcgc gaggaggtgg
     1501 cgctgcgcga ccttccggag ggaggcgtgc ccggccggcg gcgccccgcc gtgccgccgt
     1561 ccgagtatcg gccctatgag ccggacgccg acggcgttcc cccgatggcc gacttcggca
     1621 gcggctaccg gttccacgtg accgggctcg cgcacgatgc ccggggctat ccgacccagg
     1681 atcccgcgac gatcgcggct caccagcgcc ggcggctgga gaagatcacg gggtttgccg
     1741 acctggtcgc cccgacccgg gcactgtacc tcgacgacgc ggaggtcgcc gtcgtcgccg
     1801 tcggcatcgc tgcgcgggcg gcgcaggcgg cggtggacga agcgcgggga cgcggcatcc
     1861 gcgcgggcct cctgcgtccc gccgtgctct ggccgtttcc cgacgaggcg gtgcgcgagg
     1921 tcgcatcgtc tgtcagggcg atcgtcgtgg cggagatgaa cctcggccag atcgtgaggg
     1981 aggtcgaacg cgcggcgggc ggccgggcca cggtggtgcc ctgctgccgc gccgacggca
     2041 cggcgctcga acctgagcag gtgctcgcca gcctcgaagc gctgatgccc gcgggaagga
     2101 gggcgtaatg gtcgatatcc accggtacct ccgtctggag cagatgccgc acgtgctgtg
     2161 tcccggctgc agccacggca tcgtcgcggg cgcgctgctg cgggcgatcg accggctggc
     2221 gttgccgcag gaccgggttg ccgtcgtcgc cgggatcggg tgcagcagcc ggctgcccgg
     2281 ctccctggat ttctgcaccc tacacggcac ccacggccgg gcgccagcat ttgccaccgg
     2341 gttgaagctc gcccggcccg acctccacgt ggtccttatc accggggacg gagacgggct
     2401 ggccatcggc ggcaaccacc tcatccacgc cgcgcggcgc aacatcgaac tgacctgtct
     2461 tttgttcaac aacgaggtgt acgggatgac gggaggacaa ttggcgccga cgacgccgga
     2521 aggcggtgtg gccacgacgg cgccgttcgg caacgtcgag ccgccattcg agccctgccg
     2581 cctgatggcc gcggccggcg ccacgttcgt cgcgaggggc atcgcgtacg agccgacggt
     2641 cctggaggac atgatcgcgc gggggctgcg gcacaagggg ttcgccttca tcgaagtgct
     2701 cacggactgt ccggagtact acggccgcta caaccagctg ggcagcccga cggcgatgct
     2761 gtttgcccaa aagcgggccg tggcggcgat cgacgcgcaa ctggatgccg ggcacgcgac
     2821 gatcctgcac gcggcgcccg cccggcggct cgcgccgcgg gtcgagaccg gtgtcctcgt
     2881 cgagacgcac cgccccgagt tcgccgagcg cacccgggcg ctcatggctg tggcccaggg
     2941 acgccgatga cactggccgt ccgccttggg ggcgcgggag ggcaggggat cgtgctcgcc
     3001 ggcctgatcc tggccgaggc ggcggcgctg gccggccgcc gggtggtctg cacccaggcc
     3061 tacgggccgg agtcgcgcgg cggagcgagc cggtcggatg tgatcatcag cgatcagccg
     3121 atcgtgttcc cgatggcgcg gcggctcgac gtgctggtcg ccctgacgca ggaagcgtgc
     3181 gaccgctcct ggggagcgct ggcgcccaat ggtctcgcga tcatcgatgc cggccgggtg
     3241 acgctgcctc cgcgaggacc tgctgagtgc cacgccctgc cggtcaccgg cacggcccgg
     3301 ctggtcacgg ggtcgacccg gtcagcgaac atcgtggcgc tcggcgcagt ctgcggcctg
     3361 acgggtgtgg tgccgcgtcc ggcactcgag gaggccgtcg cccgccgcgt tccgtcagcg
     3421 tcccgcgggc tgaacctggc cgcgctcggc gcgggagagc ggctgggcgt cgaggcccgc
     3481 gcagcggtgg cggcaggagg tgggcggtga ccatgggtcc cgatcggggg gggcgcgcag
     3541 cgggagcgat cctggaagac gccggctccc cggccggtgg gaacacgctc gccgtccctg
     3601 cgccggcatc gctcgggacc gggcgcgaca gcaacacgcg ggcccggatt ctgcgcgccg
     3661 ccgcggcgtt gttccaccgc cagggatttg accgcaccac gatgcaggag atcgctgccg
     3721 caagcggtct gaccaagggg tcgatctatc accacatccg gagcaagcag gccctcctct
     3781 acgagatcct tcagcacacg ctcgaccgtt cgctgccgga cctcgagcgg atcgccgccg
     3841 cccggctccc ggcggccgaa cggctcaggc gggcggtccg cctgcacatc ctcaccctcg
     3901 tctccgaccg tgacaacgtg tcatgcttta tcgaggaagg acgctcgcta gcccccgagt
     3961 atcaggccgc ccacctggcg gcccgcgacc ggtacgaggc gctctttcgc cgcatcgtgg
     4021 aggacgggat cgggtccggc gagttcgcgc cgaccgatgt gcggctggcc gggctcgccg
     4081 tgctcgggat ggtgaactgg atggtgcggt ggtaccggcc cgagggcccg cgccccgcag
     4141 aggagatcgc cgcccggttc ggcgactacg cggtcggcgc gctcgcgggc gcgcgggggg
     4201 aacatgacag cccccgcgct tgacggaatc cgtgtcctcg acctctctca gggcgcagcg
     4261 gggccgatct gcacgatgca cctggccgat ctgggggccg ccgttgtcaa ggtcgaaccg
     4321 cccggagggg agtggggccg gacgctcggc ccgccgttcc tcgatggggt cgccgcggcg
     4381 ttcgtcggga tgaaccgcaa caagcggagc ctcgtcgtcg atctcaaaca ggccgcaggc
     4441 cgagaggtcg tggcgcgtct ggccgaagca agcgacgttc tcgtcgagag cttccgcccg
     4501 ggggtcatgg cccgcctgca gctcgattac gatacgctgg ccccgcggtg tccgaggctg
     4561 atctactgcg ccatctccgc cttcggccag gacgggccat ggcgcgacaa gccgggcgtg
     4621 gacggtatcg ttcaaggcgc cagtgggttg atgagcgtga ccggggagcc cggcggggcc
     4681 cccgtcaagg tcggcgtgcc ggcggcggac acggtcgcgg gcttcctggc cgcgcaggga
     4741 atcctcgccg cattgtttgg ccgcgagcgc accggccggg ggcagcgggt ggacctcgcc
     4801 ttgctggatg cactcctcgc gtttcaagcc gttccgctgg cgatgttcct ctcgtcgggg
     4861 aaagacccgg ggcgctccgg cagcgccgcc ccgtacgcgg ccccgaacga agcgttcccg
     4921 acagaggatg gcttcatcat ggttgcggcc tacacccccg ggcgatggac caaactgtgc
     4981 gaggttctcg gctgggcaaa gctcgccacc gatcctcggt ttgactcgaa cgaaaagcgg
     5041 gtgcggaatc gcgccgcgct ccgtgggatc ctgaccccga tcttccagac caggacctcg
     5101 gccgcgtggg tgtcgacgct ggaagaggcc gacatcctgt gcggccccat cgccacctac
     5161 cccgaagtcg tcgtccatcc gcaagtcgtc cacaaccgta tggtggtggc gctcgaccac
     5221 ccgcaactcg ggcgcacggc ggtcgtgggc agccccctgc ggttcagtgc cacgccgacc
     5281 gccgaggccg cgccggcacc gctggccggg gaacactccg aggcggtgtt gctgagagcc
     5341 ggattttccg gcgacgagat cgccacactg atggcgcgcc ggatcgtgca gggccccgcc
     5401 gtagcggtgg ggacatagcg ggcgcgacgg ccgcgtcccg gagaggtgat ctgagtgggc
     5461 gagacggtgc tcttcgaaca gaatgcggcg atcggcatgc tcacgctgaa ccggcccgag
     5521 gcgctcaacg cgctgacggc ggagatgctc gaccgcatag gcgagttcct cctcaaggct
     5581 gaagcgagcg gtgatgtccg cgccctcatc ctgacggccg ccggcgagaa ggcgttctgc
     5641 gtcggcgccg acctcaagag ccgggcgcag gagtacgaaa gcgacggggc cggccctgat
     5701 ccgctcggcg acgtcgtgcg ccgcgtgttc ggccagatcg aggcgcttcg cctccccgtc
     5761 atcgcggcga tcaacggata tgccctgggc ggggggctgg agcttgcgct ggcctgcgac
     5821 ctgcgcgtgg ccgccgaggg cgcacggctt gggctgccgg aggccaaggt aggcagcatg
     5881 cccggcgccg gaggcaccca gcggctggcg cgcctcatcg gcccggcgcg ggccaaggag
     5941 ttgatgttca ccggcgagtt tgtcgacgcc ggcgaggcgc accgtatcgg gctggtgaat
     6001 cgcgtcttcc cggcggcagg gctgatggag ggcgcccgag cgctcgccca aacgatcgcc
     6061 tcacgggccc cgctgtccgt ccacgcgatc aaggcggcgg tgaacctgtc ccacgacgtc
     6121 agcctcgacg cggggctggc cttcgagcgc acagcccacg cggtgctgcg cgccagtcgc
     6181 gatcggcgtg agggcatcca ggcattcgtg gaaaagcggg agccccactt cgtcggtcgc
     6241 tgagagaaag ggggaggcgc gggatgggag tccgtgggcg ggtcgcggtc gtcaccgggg
     6301 ccggccaggg catcggggaa gggatcgcgg tccatctgca cgccgccggc gcccgggtcg
     6361 tggtcaacga ccttgtcccg gagcgcgtcc agcgggtcgt ggccgagatg aacgacggcg
     6421 gcccagaggc cttaggcgtg gccgccgacg tcagccgcgc cgagggggcc gtcgcactga
     6481 tccgccaggc gcgcaaggcg ttcgggcggg tcgacattct cgtcaacaac gtcggcatcg
     6541 cccgcgaccg ctaccttacc aagatgtccg aggaagactg ggacgaggtc ctgcgcgtca
     6601 acctgaaatc ctactacctc tgctgccatg aggccgcgcc gcacatgatg gagcagcagc
     6661 acgggcggat catcaacatc tcgtcgcggg cctggctggg cgggcccgga caggcgaatt
     6721 acgcggccag caaggggggc gtggtgagcc tgacgcgcac gctggccctg gagctggcca
     6781 agtttggcat caccgcgaac gcgatcgccc ccgcgctcgt cgatacgccg ttgttccgca
     6841 gcctgaagga cgagattcag gagcggctga ccaagacggt acccgtcggg cgcatcgcca
     6901 ccccggccga catcgcgaac gcggcgctat tcttcgccag cgacgaatcg tcgtacgtca
     6961 ccgggcagct gctgtacgtt tgcggcggtc gcagcgtggg agcgtactga cgcgccgccg
     7021 gcggagggag ccgaggcgag cgcgtggagg tggtgggagt ggcagagacg atggcgagcc
     7081 cggcgggcgt tgaagcggca accccgctcc ggcccggact cttcgaggcc gatgccgcgg
     7141 ggacatggcg gttggtcggc gcccgctgcc gggcgtgtgg ggcgcac
//
LOCUS       DASQGH010000487         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39032, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000487 DASQGH010000000
VERSION     DASQGH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39032"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1086)
                     /locus_tag="VEW91_09545"
     CDS             complement(118..1086)
                     /locus_tag="VEW91_09545"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91861.1"
                     /translation="MKRVLWGVAVVALAIGIAAAIPGEGAPPTPLVFMLDWFPNPDHV
                     PLYAALGEGFFAQEGVRVTLQVPANTDDPLKLAAVGRVDVAVNYEPNVILARAQGLPV
                     RSIGVLVGQPLVTIMYLKASGIRSPKDLAGRRVGFAVTGFADAMVDQMMRTAGGAPTG
                     VRMVHVGFDLVPALLARKVDAVVGAYRNYERVQIELQGQAVGTFEPERYGVPTFYELV
                     LITSDQGLARHREALGHFVDAVARGIAFTLANPEQAFAAYVRANPRMTLDDELNRKSF
                     RLTLPTYARSQVQAAARWAEFDRWLAAHKVIPQAVPVDDLYTNLVP"
     gene            complement(1097..>1981)
                     /locus_tag="VEW91_09550"
     CDS             complement(1097..>1981)
                     /locus_tag="VEW91_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91862.1"
                     /translation="IEDPGGTSGRDGEGVPVRAALKMPEEHLPETAPSGAGWVGPAKR
                     WAAQAGPPAVLLAAIVAGWEWAVRAFGIPFYILPAPSRIARLAVADRGLLLGEAAVTL
                     LEILLGFGIALVVGIGLALLIFTSRTVERAMYPLVIASQTVPVFAIAPLLIVWLGYGM
                     LSKVAMAALIVFFPIVVNTVDGLRAADPDAVNLLLILGATPAQVLLKIRAPAALPFVF
                     SGIRIAVATSVIGAVIGEWVGATQGLGFLMIHANAQLRIDLVFAAIAVLSLMAIALFV
                     AASGAEWLLLPWRRAGNA"
BASE COUNT          325 a          732 c          669 g          255 t
ORIGIN      
        1 cggcgtttca aacgccacga gcgactccac gaggcggtag agatcctctg tgtgatcggc
       61 gaggaaccga tccagcgcgc tcgcctggga cgcggcgtcg acgaccatgg tcacgcctta
      121 ggggaccagg ttcgtgtaca ggtcatccac ggggaccgcc tgcgggatga ccttgtgcgc
      181 ggcgagccac cggtcgaact cggcccaccg ggccgccgcc tgcacctggg accgcgcgta
      241 ggtcgggagc gttagtcgga acgacttccg gttcagctcg tcatcgagcg tcatcctggg
      301 gttggcgcgg acgtacgcgg cgaaggcctg ctcggggttc gccagcgtga acgcgatccc
      361 ccgggccacc gcgtcgacga aatgcccgag ggcctcccgg tggcgcgcca gcccctgatc
      421 gctcgtgatc agcaccagct cgtagaacgt ggggacgccg taccgctccg gctcgaacgt
      481 cccgacggcc tgcccctgga gctcgatctg cacgcgctca tagttccggt aggcgccgac
      541 gaccgcgtcg accttgcgcg cgaggagcgc tggcacgaga tcgaacccga cgtgcaccat
      601 ccggacaccg gtgggcgccc ccccggcggt ccgcatcatc tgatcgacca tcgcatcggc
      661 gaaccccgtc acggcgaagc cgacgcgccg gcccgcgagg tccttcggcg accggatgcc
      721 cgaggccttg aggtacatga tggtcacgag cggctgcccc accagcacgc cgatgctgcg
      781 caccgggagc ccctgcgcgc gcgcgaggat gacgttgggc tcgtagttga ccgcgacatc
      841 gacccggccg acggccgcga gcttgagcgg gtcgtcggtg ttcgccggga cctggagcgt
      901 gacccgcacc ccctcctggg cgaagaaccc ttcccccagc gccgcgtaga gcggcacgtg
      961 atcggggttc gggaaccagt ccagcatgaa cacgagcggc gtcggtggcg ccccctcccc
     1021 ggggatcgcc gccgcgatcc cgatcgcgag cgccaccacc gccacgcccc acagcacccg
     1081 cttcatgcgc ccctcctcat gcgttcccgg cccggcgcca gggcaggagc agccactcgg
     1141 cgcccgatgc cgccacgaac agcgcgatcg ccatcaggct caacaccgcg atcgccgcga
     1201 acaccaggtc gatgcggagc tgggcgttcg catggatcat gaggaaccct agcccctggg
     1261 tcgcccccac ccactcgccg atcaccgccc cgatcacgct ggtggcaacc gcgatccgga
     1321 tgccggagaa cacgaacggg agcgcggccg gcgcgcggat cttgaggagg acctgggcgg
     1381 gcgtggcccc gaggatcagc aggaggttga ccgcatcggg gtcggccgcg cggaggccgt
     1441 ccacggtgtt gacgacgatc ggaaagaaca cgatcagggc ggccatcgcc accttggaga
     1501 gcatcccgta cccgagccac acgatgagca gcggggcgat ggcaaacacc ggcacggtct
     1561 ggctcgcgat cacgagcggg tacatcgccc gctccaccgt gcgggaggtg aagatcagga
     1621 gcgccaggcc gatcccgacc accagggcga tcccgaaccc gaggaggatc tcgaggagcg
     1681 tcaccgcggc ctcgccgagg agcagtccgc gatcggcgac cgcgaggcgc gcgatccggc
     1741 tcggcgcggg caggatgtag aatgggatcc cgaacgcgcg caccgcccac tcccaccccg
     1801 cgacgatcgc cgcgagcagg accgccggcg ggccggcctg cgccgcccac cgctttgccg
     1861 gcccgaccca cccggccccg gatggcgccg tctcggggag gtgctcctcc ggcatcttga
     1921 gcgctgccct caccggaacg ccttcgccat ctcgccctga agttccgcca ggatcttcga
     1981 t
//
LOCUS       DASQGH010000488         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30580, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000488 DASQGH010000000
VERSION     DASQGH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30580"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1224
                     /locus_tag="VEW91_09555"
     CDS             187..1224
                     /locus_tag="VEW91_09555"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HYM91863.1"
                     /translation="MNTATITALEATARALVPPGKGILAADESHSTIGGRFAAIGVVN
                     TEENRRRYRQLLFTTPGLGEFISGVILFDETIRQHADDGMPFVEVLARRGIVPGIKVD
                     TGAKPLAGSPGERVTEGLDGLRERLAEYARLGARFAKWRAVIAIEGARLPTRLAIAAN
                     AHALGRYAALCQEAGLAPIVEPDVLTDGDHSIGRCDEVTEATLHAVFDALYEHRVRVE
                     GMLLKPSMVMSGAACPHLAGVQAVAEATVRCLRRTVPAAVPGIVFLSGGQRDDLATAH
                     LDAMNRLPGPHPWELSFSYARALQAPVLAVWRGDPARAAEAQRVLYHRARCNGAARRG
                     QYTPELERTVA"
     gene            1228..2466
                     /gene="pfp"
                     /locus_tag="VEW91_09560"
     CDS             1228..2466
                     /gene="pfp"
                     /locus_tag="VEW91_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HYM91864.1"
                     /translation="MKTMALLVGGGPAPGINAVIAAATIEARNHGARVLGCHDGYRWL
                     IEGDISHVEELEIARVSRIHFDGGSILRTSRENAARSVETLRAVTGALGRLGVTHLLT
                     IGGDDTAFGAARIAQTLAGRLAVAHVPKTIDNDLPLPPEIPTFGFTTAVDVGKDLVRN
                     LMSDAATMGRWFFVTVMGRHAGHLALGIGGAAGATITIIGEEFPPGSVDLARVANILE
                     ATIIKRRAHGHDHGVAVIAEGLLERLDPETIGPVERDAYGNVRLAEMELSRLLKQRVV
                     AAFRARGLEVGIVTKELGYELRCAPPGAFDIHYCRSLGYWATRFLLDGGTDAIVTVQG
                     GRLVPMPFRDLIDAATGRIRVRYVDVASDAYRVLLAYMIRLTRQDLEDPASLRALARA
                     GALTEGAFVERFGSLVGVSS"
     gene            2492..2974
                     /locus_tag="VEW91_09565"
     CDS             2492..2974
                     /locus_tag="VEW91_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM91865.1"
                     /translation="MPISKATRVRLETQLDALPIILDGVTAELAEIHPRDGGWSAKEN
                     FAHLARHAEVFMERMDRIVREERPLLGTYRAEQDPEWPSWTHLAFDEVLERLREHRRR
                     LIVWTKSLSDDQANRVGIHPVLGAMDVGRWLEFFLMHEAHHLYFVMRRLGEARQARSR
                     "
     gene            3278..3649
                     /locus_tag="VEW91_09570"
     CDS             3278..3649
                     /locus_tag="VEW91_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91866.1"
                     /translation="MTHLSRGFLTATALFVVCALPVLAAPGVSIRALIQNPDQYDGKV
                     VSVVGTITAYRERVSAAGNPYTTFRLAEGDASVSVFIWDKQGLRDGQKVRVTGTFAKV
                     KRVGTYTFDNEIQAHRIDVLR"
     gene            complement(3668..>3967)
                     /locus_tag="VEW91_09575"
     CDS             complement(3668..>3967)
                     /locus_tag="VEW91_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91867.1"
                     /translation="SFAGTSAIGILTLTFHRATFAYGSYACSKGSCTFVGVLAGVRVK
                     GIALPINLRGVSRTATGTFPNRQAWVAAVAGWAKGHLTPQLQGQIVAEAAKIPGS"
BASE COUNT          632 a         1332 c         1388 g          615 t
ORIGIN      
        1 gccgcccaga cggcctcaag aagtataccg aatgggtccg gcgccgggta gcacgatcga
       61 gtcaaacgat cacggtggca tggggggacg ccgcccagga aatcactcca ccccggcgga
      121 agcatccgtc acgttgtcag ccgaggagac ggcgatcgaa gaacccaaga aaaaggggga
      181 ccgtttgtga acaccgcaac catcaccgcg ctcgaagcga ccgcacgcgc cctcgtgccg
      241 ccgggcaagg gcattctggc ggccgacgag agccacagca cgatcggcgg gcggttcgcc
      301 gccattgggg tggtcaacac cgaggagaac cggcgtcggt atcggcagct gctcttcacc
      361 acgccgggcc tcggcgagtt catcagcggg gtgatcctgt ttgacgagac catccggcag
      421 cacgccgacg acggcatgcc gttcgttgag gtgctggcgc gccgggggat cgtcccgggc
      481 atcaaggtcg acaccggcgc gaagccgctc gccggctcgc ccggcgagcg cgtgaccgaa
      541 gggctcgatg ggctccggga gcgcctcgcc gagtacgccc ggctcggcgc gcgcttcgcc
      601 aagtggcgcg cggtcatcgc catcgagggc gctcgcctcc cgacgcgcct tgccatcgcg
      661 gccaacgccc acgcgctcgg ccggtacgcg gcgctctgcc aggaggccgg cctcgccccg
      721 atcgtggagc cggacgtcct cacggatggg gatcactcga tcgggcgatg tgacgaggtc
      781 accgaagcca ccctgcacgc cgtgttcgac gcgctctatg agcaccgcgt ccgggtggag
      841 ggcatgctgc tgaagccgag catggtcatg tccggggcgg cctgcccgca cctggccggt
      901 gtccaggccg tcgccgaggc aacggtgcgc tgtttgcgca ggaccgtccc cgcggcggtc
      961 ccgggtatcg tgttcctgtc gggcggacag cgggacgacc tggccacggc ccacctcgat
     1021 gcgatgaacc gtttgccggg gccccatccg tgggagttga gcttctcgta cgcgcgcgcc
     1081 ctccaggcgc cggtcctcgc ggtgtggcgg ggcgaccctg cccgggccgc cgaagcgcaa
     1141 cgtgttctct accaccgcgc ccggtgcaac ggcgcggccc gccgggggca gtacacgccc
     1201 gagttggaac ggacggtggc gtagaccatg aaaacgatgg ccctcctcgt cgggggcggg
     1261 cccgcgcccg ggatcaacgc cgtcatcgct gccgcaacaa tcgaggcgcg gaatcatgga
     1321 gcgcgcgtgc tcggctgtca cgacggttac cggtggctca tcgagggaga catctcgcat
     1381 gttgaggagc tcgagatcgc ccgcgtctcg cgcattcact tcgacggcgg ctccatcctc
     1441 cgcacgtccc gcgagaacgc ggcccggtcg gtggagacgc tccgggccgt gaccggcgcc
     1501 ctcggccggc tgggcgtgac ccacctgctg acgatcggcg gcgacgatac ggccttcggc
     1561 gcagcccgga tcgctcagac gcttgccggg cggctcgccg tggctcacgt tccgaagacg
     1621 atcgacaacg acctcccact gccgccggag atccccacgt ttgggttcac gaccgcagtc
     1681 gacgtgggca aggacctcgt gcggaacctg atgagcgacg ccgccaccat gggccggtgg
     1741 ttcttcgtga cggtcatggg ccggcatgcc ggccacctcg cgctcggcat cggcggcgcg
     1801 gccggcgcga ccataaccat catcggggag gagtttccgc cgggctcggt cgacctggcc
     1861 cgcgtcgcca acatcctgga ggccacgatc atcaagcgcc gcgcgcacgg ccacgaccac
     1921 ggggtcgccg tgatcgcgga gggcctgctg gagcggctcg accccgagac catcggtccg
     1981 gtcgagcgcg acgcgtacgg caacgtccgg ctggccgaga tggagctctc gcgcctgctt
     2041 aagcagcggg tggtcgcggc ctttcgggcg cgggggctcg aggtgggcat cgtcaccaaa
     2101 gaactcggct acgaacttcg ctgtgcgccg cctggcgcgt tcgacatcca ctactgccgc
     2161 agcctgggct actgggcgac tcggttcctc ctcgacgggg gcacggacgc gatcgtgacc
     2221 gtacagggcg gccgattggt tccaatgccg tttcgcgacc tgatcgacgc ggccacgggc
     2281 cggatccgcg tccggtacgt cgacgtcgcg tcggatgcgt accgcgtcct gctggcgtac
     2341 atgatccggc tcacgcgtca ggacctggag gaccccgcga gccttcgcgc gctggctcgg
     2401 gcgggcgcgc tcacggaggg agccttcgtc gagcggttcg gctcgctcgt cggcgtgtcg
     2461 tcgtgaggca ggggcaggag ggactggcac gatgccgatc tcgaaagcaa cgcgtgtccg
     2521 tctggagacc cagctcgacg cgctgcccat tatcctcgat ggcgtgacgg ccgaactcgc
     2581 cgaaatacac ccgcgtgacg gcggctggtc ggcgaaggaa aattttgccc acctcgcccg
     2641 ccacgccgag gtcttcatgg agcggatgga tcgcattgtg cgtgaggaaa ggcctctcct
     2701 ggggacatac cgggccgagc aggatccgga gtggccgtcg tggacccacc ttgcgttcga
     2761 tgaagtcctg gagcgcctta gggagcaccg gcgcaggctc atcgtgtgga cgaagtcgct
     2821 ctcggatgat caagccaacc gcgtcggcat ccatcctgtg ctcggcgcga tggacgttgg
     2881 ccggtggctg gagttttttc tcatgcacga ggcgcaccac ttgtacttcg tcatgcggcg
     2941 ccttggcgaa gcgcgccagg cccgatcacg ataggggtcc acgtggctga cctccacgcc
     3001 gagaggcgcg atgagaagcg cagaaaggcg cggcacggca tgcgggtgag cggccggggc
     3061 gcacggctcc tcgaccgcct gctgcgagga cagggccaca aggggacccg aaaggcgggg
     3121 ggcaagcaga agaggagaaa cccgtaaggt cgcgcgggcc tcatcgacgc cggcccggcc
     3181 tccgtgaggg cggggtggcg cccgctctcg caaaccgccc cttggttatc cggtcatcgg
     3241 cggctccgca aagggtatat atgcttttga ggtggttatg acgcacctca gtcgtgggtt
     3301 cctcaccgca accgcgctct tcgtggtgtg tgcgctcccg gttctggcgg cgccgggggt
     3361 cagcatccgc gcccttatcc agaaccccga ccagtatgac ggcaaagtgg tcagcgtcgt
     3421 gggaacgatc acggcctatc gggagagggt ctccgccgcc ggcaatccgt acacgacctt
     3481 ccggctggcc gagggagatg cctcggtgtc ggtgttcatc tgggacaaac agggtctccg
     3541 cgatgggcag aaggtccggg tgacggggac gttcgcgaag gtcaagcgtg tgggcacgta
     3601 caccttcgac aacgagattc aggctcaccg catcgacgtg ctgcggtaga gtgcgctcct
     3661 gactgcccta cgagcctggg atcttggccg cctcggcgac gatctggccc tgcagttggg
     3721 gggtcaggtg gcccttggcc cagcccgcca ccgccgccac ccacgcctgc cggttgggaa
     3781 atgtgcccgt agccgtgcgg ctcacgccgc gaaggtttat cggcaacgcg atccctttga
     3841 cgcggacccc cgcgagcacc ccgacgaagg tgcacgaccc cttggagcac gcgtagcttc
     3901 catacgcaaa cgtcgcccgg tggaaggtca gggtgaggat gccaatcgcg gacgttcccg
     3961 cgaagct
//
LOCUS       DASQGH010000489         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27542, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000489 DASQGH010000000
VERSION     DASQGH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27542"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..395)
                     /locus_tag="VEW91_09580"
     CDS             complement(84..395)
                     /locus_tag="VEW91_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91868.1"
                     /translation="MMALACGLTGITIPTRAADSFSFTTIDAPGATRTWASGINDAGQ
                     IVGSFNNHGYLRTSWGAFTTFDVPGATDTGAEGINNAGQIVGTFVAGDKNHGYVATPT
                     R"
     gene            complement(521..1138)
                     /locus_tag="VEW91_09585"
     CDS             complement(521..1138)
                     /locus_tag="VEW91_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HYM91869.1"
                     /translation="MSIAPGTSIVLHQMRVPVFAAALLLVSVPIAAIAPAGQAASPEA
                     SELRCIPIEQALSPFGLPTSQDPRHIIRVGMDESSLVYSAAVPSEATDAGKTVDFTLT
                     IKNTGSTPAVFSFSTAQRYDVVIWNDDCMEVWRWSRGRMFAQAITSLSVPAGGTTVFH
                     IPWDQRDQTGHRVRVAAYESRLVFLGRRAKRSTPLVLSPLVFAVR"
     gene            complement(1206..1703)
                     /locus_tag="VEW91_09590"
     CDS             complement(1206..1703)
                     /locus_tag="VEW91_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91870.1"
                     /translation="MKRLMLLSALLTAISLVSGPLGLAAGEYTNTELKYSISLPDGWS
                     VIDNTGRETYFGRRGADYPGAAVGTLSASTRLTDDRVRHIDLNNLKVTRDTMTRQAGW
                     PARRIDGTAVSEGDALLFYSIAIKPGDDAEIIQLTVWGPPALLSSGAIGQQVDQMLAT
                     FRPVR"
BASE COUNT          391 a          638 c          658 g          378 t
ORIGIN      
        1 atccggcaga gcacctcact tgtaatgagg gggttaccgg ttcaagtccg gtcgagggct
       61 ccagcgcttg tctacggctc tgctcatcgg gttggggtcg cgacgtagcc atggttcttg
      121 tcgcccgcca caaaggtgcc cacgatctgc ccggcgttgt tgatcccctc ggcgccggta
      181 tccgtcgcgc ccgggacatc gaacgtggtg aaggcgcccc aagaggtgcg cagatagcca
      241 tggttgttga agctgcccac gatctgcccg gcgtcgttga tcccggaggc ccaagtccgc
      301 gtcgcgcccg gcgcatcgat cgtggtgaag gaaaatgaat ctgccgcacg ggtagggatc
      361 gtgatacctg ttagtccaca ggcgagcgcc atcacggcca gagcaaccca tgacagccgc
      421 ctctcgtcct ccttcgtctc agaggcttga ttcagcagtc taagatcaag tatcatccgg
      481 cgcccgcctt ggctgtcaga aatggggcgg ccgtacgacc ctaccggacc gcgaagacga
      541 gcggggagag gaccagcggc gtgctgcgct ttgctcgtcg gccgagaaag accaagcgcg
      601 attcataggc agcgacccgc acgcggtgtc cggtctggtc gcgctgatcc cacggaatat
      661 ggaacacggt ggtgccgccg gccggcacgc tgagcgacgt gatagcctgc gcgaacattc
      721 tcccccggga ccagcgccac acctccatgc aatcgtcgtt ccagatcacg acgtcgtacc
      781 gctgcgcagt cgagaatgag aacaccgccg gagtactacc ggtgttcttg atcgtgagcg
      841 tgaagtcgac ggtcttgccg gcatcagtcg cctcgctcgg taccgccgcg gaatagacga
      901 gcgaagactc gtccatgccg acccggatga tgtgccgggg gtcctgggaa gtgggaagcc
      961 cgaagggact caacgcctgc tctatgggga tgcaccgcag ctctgaagcc tcaggagaag
     1021 ccgcctgtcc tgccggcgcg atcgcggcaa ttgggacgct gaccagcagg agcgccgcgg
     1081 cgaacaccgg gactctcatc tggtgcagca cgatcgacgt ccccggcgcg atcgacactt
     1141 acgcccgtgg cataaacagc gccgggcaaa tggtagggag ttccaggccg gcgactttcg
     1201 ggcagtcagc gcacgggcct gaaggtcgcg agcatttgat cgacctgctg cccgatggcg
     1261 ccgctgctga ggagggcagg cgggccccaa accgtcaact ggattatctc ggcgtcgtcg
     1321 ccgggcttga tcgcgatgga gtagaatagc aacgcgtcgc cctccgacac cgcggtaccg
     1381 tcgatccggc gcgccggcca gccggcttgc cgggtcatgg tgtctcgcgt caccttcaag
     1441 ttgttcaagt cgatgtgtct gacgcgatca tcggtaagcc tcgtgctcgc ggagagggtg
     1501 ccgacggcgg ctccgggata gtcagcgcct cgacggccaa agtacgtctc ccggccggta
     1561 ttgtcgatga cggaccatcc gtccggcagc gagatcgagt acttcaattc cgtgtttgta
     1621 tactcgcccg ccgcaagtcc tagaggaccg gacacaagag agatagccgt aaggagtgcg
     1681 gagaggagca tcagtcgttt catgacgatc gcctcctgaa cgagcccgtg ccgtgtgtaa
     1741 tgcccttttc gcgtacactt ccagaccgca agtgagtttc tcgtaactga ggcgggcgtg
     1801 caaacgggcc cgcccccaag cacgccggag ttcctcgtgt aaatgagctt taagttgcct
     1861 gcctgttccc tagcgataat cgtacgcgga ttgagcacga tcaggcgagg ccgcagcctc
     1921 gagactgagc ctcttctccg aggaagagcg acccgtaact ccgtagtcgg ccaagcaggg
     1981 cttgacgggc ggaggttaca gctccaagtt ctcggaggcg acttggtcac tgacctgtgt
     2041 gacttgaccc agtgtgtcca ccagg
//
LOCUS       DASQGH010000490        12833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33368, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000490 DASQGH010000000
VERSION     DASQGH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33368"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1733)
                     /gene="ggt"
                     /locus_tag="VEW91_09595"
     CDS             complement(90..1733)
                     /gene="ggt"
                     /locus_tag="VEW91_09595"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYM91871.1"
                     /translation="MRGRSEGQGRSMVLAQRGLVATDHILASAAGLRVLQEGGNAIDA
                     AVCIAATLGVVTPMMAGIGGDTFIVYFQAASRQVMALNGSGAAPQGATPAFFRERGYR
                     TMPLRGMLAPSIPGAVDAMATALERWGSGRWTLGRLLEPAIYYAEAGFPITEKVAVWF
                     EKAAPVLEQYPSSGRVFLPGGRLPRVGEVLVQADLARSLRTIAEQGARAFYEGPLAGA
                     VAAYARAHGGLLDTADLAAHRSEVPQPSVTPWRDLLVYCTPPPSQGFVLLEMLNILEH
                     DEFGAQPWGTAETVHLMVEAKKLAFADRLAYVGDPRFVSNPLDYLLSKAYAREQRRRI
                     DPARAQEAVAAGAVGEQVGETTAFVVGDRDGNVVSYITSLSASFGCGEIAEGTGILLN
                     NRGGRGFSLEPGHPNLIAPGKRTMHTLMAFLATRNGRPYLAWTTRGGDAQAQWDLQAF
                     SNIVHHGLNVQEAVERPRWVSFPATDPATIDAAFELQMESGFPPQTYDRLRTLGHRVV
                     TPPPWSAQGGGVQVIRVDTDRGVYAGGSDPRFDGGAIGF"
     gene            1968..3233
                     /locus_tag="VEW91_09600"
     CDS             1968..3233
                     /locus_tag="VEW91_09600"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYM91872.1"
                     /translation="MVAPGGPGGGRGILAEAAAQTGPATVIADDVTDDATASNNQVKV
                     IRDPSGALVAAYAANAGGVPQVVLARSRDGGRRWATLAQASSGSVPSRLPALGLDGAG
                     RLHVVWTRYDDGVGKIYYRVWQGGWAAPQERISPSPTYAGFPALAVDAAGRPHVVWYG
                     IREAALPLPTRHGAIYEIFYTGFDGAKWTPPQLISTGLPDAVNPALAGDRAGRLHAAW
                     YQFDGRWYQVRYAERGASWGAPETVLATRADAFNPDLAAGPQGLIALAWEHHDGSSSV
                     VRVTRRVSGRWDDPVDLSAGSSPAHHPSVSVAPSGGVYVAWDSDDGQISVRQFTTRWE
                     PAVRLTGDGGNVYPSVAASGGGADIVWTQTARGRSSVRYLRLGPEAAGPAGPGASRTA
                     AWGGAAVLLAAAALALWRFRRLRPRPGRA"
     gene            3230..3919
                     /locus_tag="VEW91_09605"
     CDS             3230..3919
                     /locus_tag="VEW91_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYM91873.1"
                     /translation="MTRPGAPRIPAARRVAFQRRLLAWYRRHRRDLPWRRTRDPYHIL
                     VSEIMLQQTQVDRVIPKYGQWLRRYPSLESLARAGAREVREAWYPLGYNVRPVRLRDI
                     ARAALRRHRGSIPKTREELLVLRGIGPYTAGAVLSFAYRQDAPILDTNVRRVLRRVFL
                     GDRATPTDGILWTLSRALLPRGKAYSFNQALMDLGATVCTARRPRCPDCPMARFCASY
                     PLGGIPSIRCP"
     gene            complement(3906..4676)
                     /locus_tag="VEW91_09610"
     CDS             complement(3906..4676)
                     /locus_tag="VEW91_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HYM91874.1"
                     /translation="MSGLLDGKTALVMGVANRWSIAWAIARAFSREAARLIFTFQGER
                     QEKDVRELAATLPGSSVLPCDVTRDEDLAALAAAIKAEGQGVDALVHSIAFANREDLG
                     GAYVETSRAGFALALEVSAFSLVAATRHLLPVLRPGAAVVTLTYIGSSRVMPNYNVMG
                     VAKAALEASVRYLASDLGPRGIRVNAISAGPIKTASARAIQGFSRILEVMETRSPLRR
                     NTDPAEVADAAVFLCSPLARGITGEVLFVDNGFHIMGT"
     gene            complement(4673..5434)
                     /gene="nth"
                     /locus_tag="VEW91_09615"
     CDS             complement(4673..5434)
                     /gene="nth"
                     /locus_tag="VEW91_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYM91875.1"
                     /translation="MTKSATGTRGTALPPEAPAARARRAGAIARRLARRYPRAAIPLR
                     HASPFQLLIATILSAQCTDAQVNIVTRALFDRYRTAADFASARREELERAIHSTGFFR
                     AKARAIQNTSRLLLERHRGEVPRTLEELVQLYGVGRKTANVVLGAFGVPGFVVDTHVR
                     RLARRMALTAHDDPDKIEQDLMAVIPQAEWSVFSLRLVYFGREICTARGPSCPSCPLR
                     DLCPSARYGGAPPWMAPRRRRAGPAARRAGARSAP"
     gene            5531..6631
                     /locus_tag="VEW91_09620"
     CDS             5531..6631
                     /locus_tag="VEW91_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009828274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM91876.1"
                     /translation="MDYAARMNGARRLMGEQQIDLLAVSPGDDMRYLLGYVPHQDERP
                     CYLLVDSHGAVLVVPSLNAAEVAQHVSLPTLVYTDAEGPAGALAAAARTLGRGAPRRI
                     AVDDTMRADFVLTLQRAHPDAELALGSVVLAPLRMRKSGEEIEVLKRSSRHADQAMRD
                     AWAACRIGATEREIAEAAAASFARSGSQEVLFTAVASGPNGAFPHHRFTDRRLRAGDS
                     VTLDLGGRLDGYASDLTRVAYMGTPTPRYLEVYRAVEAAVTAGMAAARPGAALKEVDV
                     AARSVIERAGYGQYFIHRTGHGLGLTGHELPSVTHLNTQTIDEGMVFSVEPGIYLPGE
                     FGVRLEEIVHIARDGAHRLSELPRDVHRIAVG"
     gene            complement(6714..7577)
                     /locus_tag="VEW91_09625"
     CDS             complement(6714..7577)
                     /locus_tag="VEW91_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HYM91877.1"
                     /translation="MGVRFFLALLAAVTAVTATPVTAARGARLPIPMPAASGQASAAS
                     TASPGIVYETKTVERRDPDCQAGQGRCAYITFQYPVIVRAPSRAAADAINDAVSEFLL
                     WRLQAAPYKTIDAAMDGFMQWWLEAKAQGPPGAVPVYWEERTVKVLYGSPRIASLEFD
                     DGSFTGGAHPNYYSAYQNFDVLTGAKIHLGDILVQGYQAPLTRIAEQRFRKDKGLESG
                     ASLRQAGYTFDNDRFRLTDNFAIGPEGLTFQYNLYEIASYAQGITVILIEYGEIKTLI
                     RPDGPLGSLRR"
     gene            complement(7687..8745)
                     /locus_tag="VEW91_09630"
     CDS             complement(7687..8745)
                     /locus_tag="VEW91_09630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYM91878.1"
                     /translation="MTRSATVSNASPAASYTGLPMTPLRYSMSSTFPPPRTLSCTGPD
                     GAGARQRPRPVRIVACSMPRLSPFTMAALRAACTAGEIHRASFGKGARVDYKGRNDPV
                     TEADRAAEAAIVRTIRAAYPDHGFLGEEGGRQGEGPYTWLVDPLDGTFNYASAIPWFA
                     SSIALEHGGRLVVGVVMHTMLGEVYVAEAGRGAFAAALRDLPAQLSAWDDLSLWRRLW
                     VRNTPRLDQATLSTGFPGNVADTRLNLDHFTNLLLGAAKIRAMGSAALSLAAIALGQM
                     DGYWELGPSAWDFAAGALLVEEAGGRVTDFRGRPLDLYALQILATNGPIHDEVVAVLA
                     RGRSGLDLGGDLARGGEL"
     gene            8636..9964
                     /locus_tag="VEW91_09635"
     CDS             8636..9964
                     /locus_tag="VEW91_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYM91879.1"
                     /translation="MRGGGKVLDMLYLSGVIGKPVYDAAGEAFDTVADLVIFHGAERF
                     PRITGILLNGDRSRVAVIPWDAVAEFTASGIRLRVERRRLTPRPLQPDEILLREDILD
                     TQVVDTDDIKVVRVNDLELRQVGNEVRVVGADIGTRSLLRRLGLEPWVCRVLERVGRP
                     LSSRVIPWNLVAVLGGTLTPLRLSVSREKLKDIHPADLADLLEELPREERVEVMTALT
                     EEHAADVLEVSEPDVQATVIQELPTARASDILDEMKPDEAADVLGELPDERAEELISL
                     MEADKAEEVSRLLQYAPETAAGKMTTEFIALPDSMTADQVIMRLRETKPDSETIYYLY
                     VVDERGRLVGVLSIRSLITSPPETPISRLMRTDVVFVHPDVSAEDVAGALVKYELLAV
                     PVVEEDGRLVGIVTIDHLIDLLLEKYGPRKLGGGIDLLRRKAIREGSGAP"
     gene            9961..11235
                     /locus_tag="VEW91_09640"
     CDS             9961..11235
                     /locus_tag="VEW91_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HYM91880.1"
                     /translation="MSFSLPSFRRATSASAVRRLMLFLAIMGPGIITQNVDNDAGGIT
                     TYSLAGAGFGYTMLWTLIPITIALYVVQEMAARLGAVTGHGLADLIRERYGVRTTAFV
                     MLALLLADFGNTMAEFAGLAAGGEIFGISKYVFVPIGAVVVWLLVVKGTFRSVERVFL
                     AASAFYISYLISGALAHPNWGEVLRATVTPSFSWHADYVIMLVGVVGTTIAPWMQFYL
                     QAAVVEKRITVKEYGYTRMDVLVGSIMTDVVAWFIIVACGATIYAHHLHVDTVKDAAL
                     ALFPLAGVYAGALFGFGLINASLFSASVLPLATSYYVCEALGFQSGVDRTIRQAPVFY
                     GLYIALIIGACAVVLLPGAPLLQILFLSQVANGILLPFILVFMLLLVNRKRLMGAYTN
                     SRTFNGIAWVTVVVMILLTLALVVTQVFHIGG"
     gene            11318..12385
                     /locus_tag="VEW91_09645"
     CDS             11318..12385
                     /locus_tag="VEW91_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HYM91881.1"
                     /translation="MSDQVWTGPLPTLHDVYRARRVIAGHLPRTPLIHASALDRLLEA
                     RVYVKCENTLPTGAFKVRGGINLLHHLSAEERRRGVITASTGNHGQSIAYAGRMLGIR
                     VVIGAPRESNPLKVAAMRALGAEVILEGRDFDEAREWVERTAEQEGYRYVHSANEPLL
                     IAGVGTASLEVMEEVPDADVIIAPIGAGSGACGHCITAKVLSPRVQVVGVQAEGAPPV
                     YRSLKEGGMVRLDRMETFAEGIATRVPFALPITILRRHLDDIVLVSDEEMKLAILLLA
                     EAVRQTAEGAGAASTAAALRLRDRLRGKTVVLILSGGNIPLETLAEIYQDGARVRRAV
                     GVLRGTGTDAPASGIAAGERA"
     gene            12382..>12833
                     /locus_tag="VEW91_09650"
     CDS             12382..>12833
                     /locus_tag="VEW91_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYM91882.1"
                     /translation="MTHRELIEAAVRGERPARVPVALWRHFPGDDQRAEKLAQAHIAH
                     YKTFDVDLLKITPASGYYGDDWGLRGGYKPNREGVRTYSARPIKKATDWERLKRLDVS
                     QGVYGREAHAIRLAAEAVGAEVHILETVFSPLSIARTLAGEQASVRYLR"
BASE COUNT         1865 a         4492 c         4484 g         1992 t
ORIGIN      
        1 ggatcccgtc cggtttgatc aggaccagcg ttcgctcccg tcggacgtca gtcacgcctg
       61 attcctccgc gggcggccat gcgacatggt cagaacccga tcgcgcctcc atcaaatcgt
      121 ggatccgagc caccggcata cacgccccgg tccgtatcca cgcgaatcac ctgcaccccg
      181 ccaccctgcg cggaccacgg aggcggggtg accacccggt gcccgagcgt ccttaagcgg
      241 tcgtaggtct ggggcgggaa tccgctctcc atctgcagct caaacgccgc gtcgatggtg
      301 gccggatcgg ttgccgggaa gctgacccac cgcggccgct ccaccgcctc ctggacattc
      361 aatccgtggt gcacgatgtt actgaacgcc tggaggtccc actgcgcctg ggcgtcgccg
      421 ccccgcgtgg tccacgccag gtacgggcgc ccgtttctgg tcgccaggaa cgccatgagg
      481 gtgtgcatcg tgcgcttgcc cggggcgatg aggttggggt gtccgggctc gaggctgaac
      541 ccccgccccc cacggttatt gaggaggatc ccggtgcctt ccgctatctc tccgcacccg
      601 aaggacgccg acaggctcgt gatgtagctg acgacgttgc cgtcgcggtc cccaacgacg
      661 aacgccgtcg tctcgccgac ctgttccccg accgcgccgg ccgcgaccgc ttcctgcgcc
      721 cgtgcgggat cgatcctgcg gcgctgctcg cgagcgtagg ccttgctgag gaggtagtcg
      781 agggggttcg agacgaaccg cggatctcca acgtaggcga ggcggtcggc gaacgccagc
      841 ttcttggcct cgaccatgag gtggaccgtc tccgccgtcc cccagggctg ggctccgaac
      901 tcatcgtgct cgaggatatt gagcatctcc aggagcacaa acccctggga gggaggcggc
      961 gtgcagtaga cgaggaggtc gcgccagggc gtgacactcg gttgcgggac ctcgcttcga
     1021 tgcgcggcga ggtctgcggt gtcgagcaga cccccgtgcg ccctggcgta cgccgcgacc
     1081 gcccctgcga gcggcccttc gtagaacgcc cgcgcgccct gctcggcgat ggtgcgcaat
     1141 gatcgcgcca gatcggcctg cacgaggacc tcgcccaccc tgggaagccg cccacccggc
     1201 aggaagaccc gcccggacga cgggtactgc tcgagcacgg gggcggcctt ctcgaaccac
     1261 acggccacct tctccgtgat ggggaatcct gcttccgcgt agtagatcgc cggttcgagc
     1321 agccggccca gggtccaccg gccgcttccc cagcgctcga gggcggtcgc catcgcgtcc
     1381 accgcgcccg ggatcgaagg cgcgagcatt ccccggagcg gcatcgtccg gtagccgcgc
     1441 tctcggaaga acgccggcgt cgctccctga ggcgccgcgc cgcttccgtt gagggccatc
     1501 acttgtctcg atgcagcctg aaagtagacg atgaaggtgt cgcccccgat gccggccatc
     1561 atcggggtca ccacccccag cgtggcggcg atgcacacgg cggcatcgat cgcgttcccg
     1621 ccttcctgga gcacccgcag gccggccgcc gacgcgagga tgtggtcggt cgccacgagg
     1681 ccgcgctgcg ccaggaccat gctccggcct tgcccctcgc tgcggcccct catcgatggt
     1741 cccccacggg agctctcgct tcctatgatc tcatctgcgc gttggctcaa ggctcctcgc
     1801 ctcgtccatt atactgaatc cgggtggggc cgggtggatc ggttcgcccc ctgcgcgcct
     1861 gaagcgccgc cgagcaggga tgcgtttcgg ccttcgccaa tcctctcgcc gtgcgagccc
     1921 ggcccccgct ttccgcgttg gcgcttctga ttgtgcttgt ttgccctatg gtggcccccg
     1981 gcggccctgg cggcggccgc ggcattctcg ccgaggccgc cgcccagacg ggcccggcga
     2041 cggtcatcgc cgacgacgtc acggatgatg cgaccgcgtc gaacaaccag gtcaaggtga
     2101 tccgcgatcc ctcgggcgcg ctcgttgcgg cctatgcggc aaacgccggc ggcgtcccgc
     2161 aggtggttct ggccaggtca cgagacggcg gcaggcgctg ggcgacgctg gcccaggcga
     2221 gcagcggctc ggttccgtcc cggctccccg cgctcggcct cgacggggcc ggccggctcc
     2281 acgtggtgtg gacccggtac gatgacggcg tcggcaagat ctattatcgg gtctggcagg
     2341 gcgggtgggc cgccccccag gaacggatct cgccgtcccc gacctacgcg ggctttcccg
     2401 ctctggccgt cgatgccgcg ggccggccgc acgtcgtctg gtacgggatc cgcgaagccg
     2461 cgctccccct ccctacgcgc cacggcgcga tctacgagat cttctacacc gggttcgacg
     2521 gcgcgaagtg gaccccgccg cagctcatct cgaccggcct tcccgacgcg gtgaatcccg
     2581 ccctggccgg cgaccgtgcg ggccggctgc acgcggcctg gtaccagttc gacggccgct
     2641 ggtaccaggt gcgctacgcg gagcgcggcg cgtcgtgggg ggcgccggag accgtgctgg
     2701 ccacgcgggc cgatgcgttc aaccccgacc tggcggcggg gccgcagggg ctgatcgcgc
     2761 tcgcatggga acatcacgat ggatcgtcgt cggtggtccg ggtgacgcgg cgagtttcgg
     2821 gccggtggga cgaccccgtg gatctctcgg ccggttcctc gccggcgcac catccttcgg
     2881 tgagcgtcgc gccgtctgga ggcgtgtacg tggcctggga cagtgacgac ggccagatct
     2941 cggtgcgcca gttcacgacc cggtgggagc ccgcggttcg cctgaccggc gatggcggca
     3001 acgtgtaccc aagcgtcgcg gcttccgggg gcggggccga catcgtgtgg acccagaccg
     3061 cgcgggggcg ctcgtcggtc cgctacctcc ggctgggacc cgaggccgcc gggccggcgg
     3121 gtcccggcgc ctcccgcacg gcggcatggg gaggtgcggc cgtcctgctc gcggcggcgg
     3181 cccttgccct gtggcgtttc cgccgccttc gaccgcggcc ggggcgggca tgacgcggcc
     3241 gggggcgccg cggatacctg cggcccggcg cgtggcgttt cagcgccggc tgctggcgtg
     3301 gtaccggcga caccggcgcg atctcccgtg gcggcggacg cgcgacccgt atcacatcct
     3361 cgtctccgag atcatgctcc agcagacgca ggtggaccgc gtcatcccca agtacgggca
     3421 gtggctccgc aggtatccgt cgttggaatc cctcgcccgg gccggcgcgc gcgaagtccg
     3481 ggaggcctgg tatccgctcg gctacaacgt ccggccggtg cgcctgcgcg acatcgctcg
     3541 cgcggccctc cgccgccacc ggggcagcat ccccaagacg cgggaggagt tgctggtcct
     3601 cagggggatc gggccctaca ccgcaggggc ggtgttgagc tttgcgtacc ggcaggacgc
     3661 gccgatcctc gacaccaacg tgcgccgcgt cctccggcgg gtgttcctgg gggaccgcgc
     3721 caccccaacg gacgggatcc tctggacgct ttcccgggcg ctgctgccgc gcggcaaggc
     3781 gtacagtttc aatcaggcgt taatggacct gggagcgacc gtctgcaccg cgcgccgccc
     3841 ccggtgtcca gactgcccca tggcgcggtt ctgcgcgtcc taccccctgg gcgggatccc
     3901 ctcgatcagg tgcccatgat atggaagccg ttgtcgacga acaggacttc cccggtgatc
     3961 ccccgggcga gcgggctgca caggaacacc gccgcgtccg ccacctcggc gggatcggtg
     4021 ttccggcgga gcggcgaccg cgtttccatg acctcgagga tccggctgaa tccctggatc
     4081 gccctggctg aggcggtctt gatcgggccc gctgagatcg cgttcacgcg gatcccgcgc
     4141 gggccgaggt cgctggcgag gtagcggacc gaggcctcca gcgcggcctt cgcgaccccc
     4201 atcacgttgt agttgggcat gacccggctc gagccgatgt aggtgagtgt cacaacggcg
     4261 gctccgggcc tgaggaccgg gagcagatgc cgcgtcgcgg cgacaagcga aaacgcgctg
     4321 acctcgaggg ccagggcgaa tccggcccgc gaggtctcca cgtaggcgcc gccgaggtct
     4381 tcacggttgg cgaaggcgat gctgtggacg agcgcgtcca cgccctggcc ctccgccttg
     4441 atggcggccg ccagggccgc caggtcctcg tcccgggtca catcgcatgg gaggacggac
     4501 gagcccggga gggtggcggc gagctcgcgc acatccttct cctggcgctc gccctgaaac
     4561 gtgaagatca ggcgggcggc ctcccgcgaa aacgcccggg cgatggccca cgcgatgctc
     4621 cagcggttgg ccacccccat cacgagcgcg gtcttcccat cgagcagccc gctcatggcg
     4681 cgctcctcgc ccccgcgcgc cgcgcggccg gaccggcgcg gcggcggcgc ggcgccatcc
     4741 acggcggcgc tcccccgtac cgcgcggacg ggcagaggtc gcggagcggg caggacgggc
     4801 agctggggcc gcgggcggtg cagatctcac ggccaaagta gaccagccgc agcgagaaca
     4861 cgctccactc ggcctgcggg atcacggcca tgaggtcctg ctcgatcttg tcggggtcgt
     4921 cgtgcgcggt cagcgccatc cggcgggcga gccgccgcac gtgggtgtct acgacgaagc
     4981 cgggcacgcc gaaggcgccg aggacgacgt tggccgtctt gcggcccacg ccgtagagtt
     5041 gcacgagctc ctccagcgtg cgggggacct cgccgcggtg tcgctcgagg agcagccggc
     5101 tggtgttctg gatcgcccgc gccttggccc ggaagaatcc ggtgctgtgg atcgcccgct
     5161 cgagttcttc gcggcgcgcg gaggcgaagt ccgctgcggt gcggtagcgg tcgaacagcg
     5221 ccctggtgac aatgttcacc tgcgcgtccg tgcactgcgc ggacaggatt gtggcgatca
     5281 ggagctggaa gggggaggcg tgccgcagcg ggatcgccgc gcggggatac cggcgggcga
     5341 gccgccgcgc gatcgccccg gcgcgccggg cccgggccgc cggcgcctcg ggcggcaggg
     5401 ccgtcccgcg cgtcccggta gcgctcttcg tcacgtgttc actctaccgt ggtccccggt
     5461 gggtgtcaat ttaggggaga ggaggtcttg gctgcgggaa gaagcggaat acatctggga
     5521 ggtggatgcg atggactacg cggcgcggat gaacggagcc cggcggctca tgggtgagca
     5581 gcagatcgac ctgctggcgg tctcccccgg cgacgacatg cggtacctgc taggctacgt
     5641 gccgcaccaa gacgagcggc cgtgctacct gctggtggat tcccacggcg cggtgctggt
     5701 ggttccttcg ctcaacgccg ccgaggtcgc gcagcacgtg tcgctcccga cgttggtata
     5761 caccgacgcc gaggggcccg ccggagccct ggccgccgcc gcccgaaccc tcgggcgggg
     5821 ggcgccgcgc cggatcgccg tcgacgatac gatgcgggcg gacttcgtgc tgacgctcca
     5881 gcgggcgcat ccggatgccg agctcgcatt gggctccgtg gtccttgccc ccctccggat
     5941 gcgaaagtcg ggcgaggaga tcgaggtgct caagcggagc agccggcacg ccgaccaggc
     6001 gatgcgcgat gcctgggccg cgtgccgcat cggagccacc gagcgggaga tcgcggaggc
     6061 cgcggcggcg tcgttcgcgc gctccgggtc tcaagaagtg ctcttcaccg cggtggcgtc
     6121 cggcccaaac ggcgcattcc cccaccaccg cttcaccgac cggaggctcc gcgcgggcga
     6181 ctcggtcacc ctggacctcg gcggccgcct ggacgggtac gcctccgacc tcacgcgagt
     6241 cgcgtacatg ggcacgccga ccccgcggta cctggaggtc taccgggcgg tggaagccgc
     6301 cgtgaccgcc ggcatggctg cggcgcggcc gggggcggcg ctcaaggagg tggacgtcgc
     6361 cgcccggagc gtgatcgagc gcgcagggta cggccagtac ttcatccacc ggaccggcca
     6421 cgggctcggg ctcaccgggc atgaactgcc gagcgtcacc catctcaaca cgcagacgat
     6481 cgacgaaggc atggtgttct cggtcgagcc ggggatctac ctccccggcg agttcggcgt
     6541 ccggctggag gagatcgtgc acatcgcccg ggacggcgcg catcgcctca gcgaacttcc
     6601 ccgcgacgtc catcggatcg ccgttggata aggctgttcc ggccgagcag cgctcccagc
     6661 cggtttatcc acacggacag cgatgaggta gccgaaccta agcagggcga aggctagcgt
     6721 cgcaacgagc ccagcggccc gtccggcctg atcagggtct tgatctcgcc atactcgatg
     6781 aggatcaccg tgatcccttg agcatacgac gcgatctcgt acaggttgta ttggaaggtg
     6841 agcccctcgg gaccgatcgc gaagttgtca gtgaggcgga accgatcgtt gtcgaacgtg
     6901 tacccggcct gccgcaggct ggcgcccgat tctaaccctt tgtccttccg gaacctttgc
     6961 tccgcgatcc gcgtgagcgg ggcctgatac ccctggacca ggatgtcccc gaggtggatc
     7021 ttcgccccgg tcaggacgtc gaagttctga taggcggagt agtagttggg atgcgcgccg
     7081 ccggtaaacg acccgtcgtc gaactccaga ctggcgatgc gcggcgaccc gtagaggact
     7141 ttgacggtcc tctcttccca gtagacgggc acggcgcctg gcggcccctg cgctttcgcc
     7201 tcgagccacc attgcatgaa cccatccatc gcggcgtcga tggtcttgta tggcgccgcc
     7261 tgaaggcgcc agagaaggaa ctcgctgacc gcgtcgttga tcgcgtctgc tgccgcccgg
     7321 ctcggggccc ggacgatgac aggatactgg aaggtgatgt aggcgcacct gccctgcccg
     7381 gcctgacagt cagggtcgcg ccgctccact gtcttcgtct cgtagacgat ccccggcgat
     7441 gcggtgctcg ctgcgctcgc ttggcccgag gccgccggca tagggatagg aaggcgcgcc
     7501 ccacgggccg ccgtcaccgg cgtcgcggtg acggcggtga cagcagcgag aagcgcaagg
     7561 aagaatctca ctcccatgat cctatcgctt ctatgttccc cgctcgggtc gggggaagac
     7621 ctcgccgccc gcgctgattc ctcgcgcgcg ctgggtcgag cgtgcaaaag ccccgaaaca
     7681 gtttagctag agctctccgc cgcgtgcgag gtcgccgccc aagtccaatc cgctccgtcc
     7741 tctggcgagg accgcgacga cctcgtcgtg gatcggcccg ttggtcgcca ggatctgaag
     7801 cgcgtacagg tccaacggtc ggccccggaa gtccgtgacg cggccgccgg cttcttccac
     7861 gagcagcgcc ccggccgcga aatcccaggc gctggggccg agctcccagt acccgtccat
     7921 ctggccgagc gcgatcgcgg ccaggctcag cgccgcggat cccatcgccc ggatcttggc
     7981 cgcccccagg aggaggttcg tgaagtggtc gaggttgagc cgggtgtccg ctacgttgcc
     8041 gggaaatccg gtcgagagcg tggcctggtc cagccgtggc gtgtttcgaa cccacagccg
     8101 ccgccacagc gaaagatcgt cccacgcgga cagctgagcg ggcaggtcgc gcagggccgc
     8161 ggcgaacgcg ccccggcccg cctccgcgac gtacacctcg cccagcatcg tgtgcatgac
     8221 gacgccgaca accaggcggc ccccatgctc gagcgcgatg ctcgaggcga accacgggat
     8281 cgcgctcgcg tagttgaacg tgccgtccag cggatccacc agccaggtgt acggcccctc
     8341 gccctgccgg ccgccttcct cgccgagaaa cccgtggtcg ggataggcgg cccggatcgt
     8401 ccgaacgatg gccgcctcag cggcccggtc ggcctcggtg accgggtcgt tccgcccctt
     8461 gtagtccacc cgagccccct ttccaaagga ggcgcggtgg atctctccgg cggtgcacgc
     8521 ggcccgcagc gcggccatcg tgaagggcga aagcctcggc atgctacagg ccactatacg
     8581 caccgggcgg ggccgttgtc gagcgccggc accatccggg cccgtgcagg agagcgtgcg
     8641 cggaggcgga aaagtgctag acatgctgta cctcagcggg gtcatcggca agcccgtgta
     8701 tgatgcggcc ggggaggcgt ttgacacggt cgccgacctc gtcatctttc acggggcgga
     8761 gaggttcccg cgcatcaccg gaatcctgct gaacggcgat cgcagccggg tcgcggtcat
     8821 cccctgggat gccgtcgcgg aattcaccgc aagcggcatc cggctccggg tcgagcgtcg
     8881 gcgcctgacc ccgagacccc tccagcccga cgagatcctc ctcagggagg acatcctgga
     8941 cacgcaggtc gtggacaccg acgacatcaa ggtggtccgc gtgaacgacc tggagctccg
     9001 ccaggtggga aacgaagtgc gcgtggtggg cgcggacatc gggacccggt ccctgctccg
     9061 ccggctcggg ctcgaaccgt gggtgtgccg cgtgttggag cgcgtggggc ggccgctgtc
     9121 gtcccgggtg attccgtgga acctcgtggc ggtgttgggg gggacgctga cccccctcag
     9181 gctcagcgtc tcccgtgaga aactgaagga tatccatccg gccgacctgg cggacctgct
     9241 cgaagagctg ccccgcgagg agcgggtcga ggtgatgacg gccctgaccg aggagcacgc
     9301 ggcggacgtc ctggaggtgt ccgagccgga tgtgcaggcc accgtcatcc aggaactgcc
     9361 gaccgcacgg gcctccgaca ttctggacga gatgaagccc gacgaggcgg cggacgttct
     9421 cggcgaactt ccggacgagc gcgccgagga actgatctcc ctcatggaag ccgacaaggc
     9481 cgaggaggtc agccggctgc tccagtacgc gcccgagacc gcggcgggga aaatgacgac
     9541 ggagttcatc gcgctcccgg actcgatgac cgccgaccag gtcatcatgc gcctccggga
     9601 gaccaagccg gattcggaga ccatctacta cctctatgtc gtcgacgaac ggggtcgcct
     9661 cgtgggcgtg ctctcgatcc ggtcgctgat cacctccccc cccgagactc ccatctcccg
     9721 gctgatgcgc accgacgtgg tcttcgtcca tccggacgtg agcgccgagg acgtcgccgg
     9781 cgcgctcgtc aagtacgagc tgctggcggt gcccgtcgtg gaggaggacg gccggctcgt
     9841 ggggatcgtc acgatcgacc acctgatcga cctgctgctc gagaagtacg gcccccgcaa
     9901 actcggcggc gggatcgacc tcctccgccg gaaagcgatc cgcgaaggct caggggcccc
     9961 atgagtttct ccctccccag cttccgccgc gctacgagcg cttcggcggt gcgccggctc
    10021 atgctgtttc tggccatcat ggggcccgga atcatcaccc agaacgtcga caacgatgcc
    10081 ggcgggatca ccacctattc cctggccggg gcggggttcg gctacacgat gctctggacg
    10141 ctgatcccga tcaccatcgc gctctacgtc gtccaggaga tggcggcgcg gctcggcgcg
    10201 gtgaccgggc acgggctggc cgacctcatc cgcgaacggt acggggtccg caccaccgcc
    10261 ttcgtgatgc tcgcgttgct cctggccgac ttcggcaaca cgatggccga gttcgcgggg
    10321 ctcgcggctg gcggcgagat tttcgggatc tccaagtacg tcttcgtccc catcggcgcg
    10381 gtggtcgtct ggctgctcgt ggtgaagggc acgttccgct cggtcgagcg ggtgtttctc
    10441 gcggcgagcg cattctacat ttcctacctg atctccggcg cgctggcgca tcccaactgg
    10501 ggcgaggtcc tccgggccac ggtcacgcct tcttttagtt ggcatgcgga ttacgtcatc
    10561 atgctcgtgg gagtcgtcgg gacgacgatc gcgccctgga tgcagttcta cctccaggcg
    10621 gccgtcgtgg agaagcggat caccgtcaaa gagtatgggt acacccggat ggacgtattg
    10681 gtcggctcga tcatgaccga cgtcgtcgcg tggttcatca tcgtcgcgtg cggggcgacg
    10741 atctacgccc accacctcca cgtggacacc gtcaaggacg cggcgctcgc gctcttcccg
    10801 ctcgccggcg tctacgcggg cgcgctcttc ggattcggtc tcatcaacgc gtcgctcttc
    10861 tccgcgtccg tgctcccgct cgccacctcc tactacgtct gcgaggccct gggctttcaa
    10921 tcgggcgtgg atcggaccat ccggcaggcg ccggtcttct acggcctcta catcgccctc
    10981 atcatcggcg cgtgcgcggt ggtgctcctc ccgggcgcgc cgctgctcca gatcctgttc
    11041 ctctcccagg tcgccaacgg catcctcctg ccgttcatcc tggtgttcat gctcctgctc
    11101 gtcaaccgga agcggctcat gggggcgtac accaactccc ggacgttcaa cggcatcgcg
    11161 tgggtcaccg ttgtcgtcat gatcctcctg accctggcgc tggtggtgac ccaggtgttc
    11221 cacataggag gctgagggcc aagcccccag gcggcccatg ccgtcgcagg ggagtggcag
    11281 gggattgcga cgatgcagag gtactatcac cacggcgatg tctgaccagg tctggaccgg
    11341 ccccctgccc acgttgcacg acgtctaccg ggcgcgaagg gtgatcgcag ggcacctgcc
    11401 ccgtacgccg ctcatccacg cctccgcgct cgaccggctg ctcgaggcgc gcgtctacgt
    11461 gaagtgcgag aacaccctgc cgaccggcgc cttcaaggtc cggggcggga tcaacctcct
    11521 ccaccacctc tcggccgagg agcggcgccg cggggtgatc accgcgagca ccgggaacca
    11581 cgggcagtcg atcgcgtacg cggggcgcat gctcgggatc cgggtcgtca tcggggcccc
    11641 ccgggagagc aacccgctca aggtggcggc gatgcgcgcg ctgggcgcgg aggtgatcct
    11701 ggaggggcgg gacttcgatg aagcgcgcga gtgggtcgag cggacggccg agcaggaggg
    11761 ctaccggtac gtccactccg ccaacgagcc cctgctcatc gccggggtgg ggaccgccag
    11821 cctggaagtg atggaggagg tccccgacgc cgacgtcatc atcgcgccga tcggcgcggg
    11881 cagcggcgcg tgcggccact gcatcaccgc gaaggtcctg agcccccgcg tgcaggttgt
    11941 cggcgtgcag gccgagggcg cccccccggt ctaccggtcg ctcaaagagg gaggcatggt
    12001 acgtctcgac cgcatggaga cgttcgccga ggggatcgcc acgcgggtgc cgttcgcgct
    12061 cccgatcacg atcctccgcc gccacctcga tgacatcgtg ctggtgagcg acgaggagat
    12121 gaagctggcg atcctcctgc tcgccgaggc cgtccggcag accgctgaag gcgccggcgc
    12181 ggccagcacc gcggccgcgc tccggctgcg ggaccgcctc cggggcaaga cggtggtgct
    12241 catcctcagc ggggggaaca tcccgctgga gaccctggcg gagatctacc aggacggggc
    12301 ccgggtccgg cgggccgtcg gggtgctgcg cgggaccggg acggacgccc cggcgtccgg
    12361 catcgcggcg ggggaacggg catgacccac cgcgagctca tcgaagccgc cgtccggggg
    12421 gagcggcccg cgcgcgtccc ggtcgcgctc tggcgccatt tccccggcga cgaccagcgg
    12481 gccgaaaagc tcgcgcaggc ccacatcgcc cattacaaga ccttcgacgt cgacctcctg
    12541 aagatcaccc cggcgagtgg gtactacggg gacgactggg ggttgcgggg gggatacaag
    12601 ccgaaccgcg aaggggtgcg cacctactcc gcccgcccga tcaagaaggc cacggactgg
    12661 gagcggctca agcggctcga cgtgagccag ggggtgtacg gccgcgaggc ccacgcgatc
    12721 cggctcgcgg ccgaggcggt cggggccgag gtgcacatcc tggagacggt gttcagcccg
    12781 ctcagcattg cccggaccct ggcgggggag caggccagcg tccggtacct gcg
//
LOCUS       DASQGH010000491         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39038, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000491 DASQGH010000000
VERSION     DASQGH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39038"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VEW91_09655"
     CDS             <1..380
                     /locus_tag="VEW91_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYM91883.1"
                     /translation="AYLKGFWEYPGDPWEFPGFYLERSPITYVRQIRTPLLILHSEND
                     LRCPIEQGEQLFVALKKQGTPTLFVRFPGESHDLSRSGQPKHRTERLRHILAWFGTYL
                     APRPSAGEAAPPARRQEGVAAGE"
     gene            387..944
                     /locus_tag="VEW91_09660"
     CDS             387..944
                     /locus_tag="VEW91_09660"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYM91884.1"
                     /translation="MTRIYTRTGDTGETSLFGGGRVPKDHPRVRAYGTVDELNAVLGL
                     ARAAGPPQEIDVLLERLQHQLFDLGAELATPDASRQAAAHVTRVTPDRVAALERDIDR
                     FEALLPPLRQFVLPGGTPAASALHHARTVARRAERAIVRLGSREPINPELLKYVNRLS
                     DLLFVLARAANHAAGRGDVTWTSSG"
     gene            complement(960..1814)
                     /locus_tag="VEW91_09665"
     CDS             complement(960..1814)
                     /locus_tag="VEW91_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91885.1"
                     /translation="MHFTLSDLLWVFFIVSFLQPVITRTLQQTARLRLFQLLERSRGT
                     RVIALIHREETMSLLGFPIVRYIDIQDSEEVLRAIRLTPPELPIDVILHTPGGLVLAA
                     EQIASALHDHPGRVTVFVPHYAMSGGTLIALSADEIVMDPHAVLGPVDPQLGTYPAVS
                     VLAAVGRKPIGRVDDETLILADIAQKSLRQVRGAVVGLASRRLAADQAEVLADALTGG
                     KWTHDYPITVAEARSLGLAVSTEVPRVIYDLMQLHPQAGRRRPSVEYVPLPYRAPVPP
                     LPRGREPR"
     gene            complement(1842..>3198)
                     /locus_tag="VEW91_09670"
     CDS             complement(1842..>3198)
                     /locus_tag="VEW91_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HYM91886.1"
                     /translation="DAYYGAQTARAIENFPISGLRLPRAFIAATATIKRAAAEVNAAL
                     GLLEATVAGAIARAAQEVAEGKWDAQFPIDAFQMGAGTSQNMNTNEVIANRAIELLGG
                     TRGDYKLVHPNDHVIMAQSTNDVTPTAIRLAALATLGDLLSALENLVEALGQKAHEFD
                     GIVKAGRTHLQDAVPVRLGQEFGAYAQAVATDVERIKNARERLLYIGIGGTATGTGLN
                     ADPQYHPRMVRRLSELTGLPLRSSGNLFESMQNMADTLECSAALRTLAATLTRIANDL
                     RLMASGPNTGLDEIRLPPVQPGSSIMPGKINPSMAEMLNMVCFHVMGNDLAVLTAVQA
                     GQLELNVMMPVIALNLLQSIQILTNGVRAFTERCVRGIVANAERCRHWVEHSAALATA
                     LNPYLGYSTAAEVVKEFVRTGRPIREIILDRGLLSEGQLNEILSVRAMTEPGIPGKAR
                     V"
BASE COUNT          468 a         1107 c         1133 g          490 t
ORIGIN      
        1 tcgcatacct gaaggggttc tgggagtatc ccggcgaccc atgggagttc ccagggttct
       61 acctggagcg atcgccgatc acgtacgtgc ggcagatccg gacgccgctc ctgatcctcc
      121 acagcgagaa cgacctgcgc tgcccgatcg agcaggggga gcagctcttt gtggcgctca
      181 agaagcaggg gaccccaacg ctgttcgtgc ggttccccgg cgagagccat gacctctccc
      241 ggagcgggca gcccaagcac cgcaccgagc ggctgcgaca catcctggcc tggttcggca
      301 cctacctcgc gccccgcccg agcgcgggcg aggccgcgcc ccctgcccgg cggcaggagg
      361 gtgttgcagc gggcgagtga gggcccgtga cgcggatcta caccaggacc ggcgataccg
      421 gggaaacgag cctgttcggc ggggggcgcg tcccgaagga ccacccgcgg gtccgcgcgt
      481 acgggacggt cgacgaactg aacgcggtgc tcgggctggc ccgcgcggcc ggtcctcccc
      541 aggagatcga cgtcctcctg gaacgcctgc agcatcagtt gttcgatctg ggggccgagc
      601 tggccacccc cgacgcctcg cgtcaggcgg ccgcccacgt gacccgggtc acgccggacc
      661 gggtggccgc gctcgagcgg gacattgatc gctttgaggc gctgctgcca cccctccgcc
      721 agtttgtgct tcctggaggc acccccgcgg cctccgccct ccaccacgcg cgcacggtcg
      781 cccgccgggc ggagcgcgcg atcgtgcggc tcggatcgcg cgagccgatc aaccccgagc
      841 tgctgaagta cgtgaaccgg ctctccgatc tgctcttcgt cctggcgcgg gcggcaaacc
      901 acgctgccgg ccggggcgac gtcacctgga cctcttctgg gtaggcgctc gctggcgctc
      961 tacctgggct cccggccgcg ggggagggga gggaccggcg cgcggtaggg gagcgggacg
     1021 tactccaccg acggccggcg ccgtccggcc tgtggatgca gctgcatcag atcgtagatg
     1081 acccgcggca cctcggtcga gaccgccaac cccaggctcc gggcttccgc cacggtgatc
     1141 ggatagtcgt gggtccactt cccgcccgtc agggcgtcgg ccagaacctc ggcctggtcg
     1201 gccgccaacc gccgcgacgc cagcccaacg accgccccgc ggacctgccg cagggatttc
     1261 tgggcgatgt ccgccaggat gagcgtttcg tcgtccaccc gcccgatcgg cttgcggccg
     1321 accgcggcca ggaccgagac cgccggatag gtgcccagtt gcggatccac cgggccaagc
     1381 acggcgtgcg gatccatgac gatctcgtcc gccgaaagcg cgatgagggt gccgcccgac
     1441 atcgcgtagt gcggaacaaa caccgtgacc cggccgggat ggtcgtgcag ggcggaggcg
     1501 atctgctccg ccgccagcac caaccccccg ggcgtgtgga ggatgacgtc gatcggcagt
     1561 tccggcggcg tcagccggat cgcgcggagc acctcctcgg aatcctggat atcgatgtag
     1621 cggacgatgg gaaacccgag gaggctcatc gtctcctcgc ggtggatcag ggcgatgaca
     1681 cgggtgccgc ggctgcgctc cagcagctgg aacagccgga gccgggcggt ctgctggagc
     1741 gtccgggtga tcaccggctg gaggaacgag acgatgaaga atacccacag cagatccgaa
     1801 agggtaaaat gcatcgcgcg cctcccttgc cagtgcgccg gctagacccg tgccttcccc
     1861 ggaatccccg gttcggtcat cgcgcgcacg gacaggatct cgttgagctg cccttcggac
     1921 aggagccccc ggtccaggat gatctcccgg atcggccgcc cggtccgcac gaactccttc
     1981 accacctcgg cggcggtgct gtagccgagg taggggttga gcgccgtcgc gagcgcggcg
     2041 ctgtgctcga cccagtggcg gcaccgctcc gcgttggcca cgatgccccg gacgcaccgc
     2101 tcggtgaacg cgcggacgcc gttggtgagg atctggatcg actgcagcag gttgagcgcg
     2161 atcaccggca tcatgacgtt gagctcgagc tggcccgcct ggaccgcggt cagcacggcg
     2221 aggtcgttgc ccatgacgtg gaagcagacc atgttcagca tctcggccat cgacgggttg
     2281 atcttgccgg gcatgatcga ggatcccggc tgcaccgggg gcaggcggat ctcgtccagg
     2341 ccggtgttgg ggcccgaggc catcaggcgc agatcgttgg cgatccgtgt cagcgtggcc
     2401 gcgagcgtcc ggagcgcggc cgagcactcc agcgtgtcgg ccatgttctg catcgactcg
     2461 aagaggttgc ccgaactccg cagcgggagg ccggtcagtt cggagaggcg gcgcaccatc
     2521 cgggggtggt actgggggtc ggcgttgagc ccggtccccg tcgccgtgcc gccgatgccg
     2581 atgtacagca gccgttcccg ggcgttcttg atccgctcga cgtcggtggc gaccgcctgg
     2641 gcgtacgcgc cgaactcctg tcccagccgg accggcacgg cgtcctggag gtgggtgcgg
     2701 ccggctttca cgatcccgtc gaactcgtgg gccttctgcc ccagggcctc caccagattc
     2761 tcgagcgccg acagcagatc ccccagggtc gcaagcgcgg cgagacggat cgcggtcggg
     2821 gtgacgtcgt tcgtggactg ggccatgatg acgtggtcgt tcgggtggac gagcttgtag
     2881 tccccccgcg ttccgccgag cagctcgatc gcgcggttgg cgatgacctc gttcgtgttc
     2941 atgttctggg aggtgcccgc ccccatctgg aaggcgtcga tcgggaattg ggcgtcccac
     3001 ttcccctccg cgacctcctg ggccgcccgg gcgatcgccc cggcaaccgt cgcctcgagc
     3061 aggcccaggg ccgcgttgac ctccgcagcc gcccgcttga tcgtcgcggt ggcggcgatg
     3121 aacgcccgtg ggaggcgcag gccgctgatg gggaagttct cgatggcccg cgcggtctgc
     3181 gcgccgtagt aggcgtcg
//
LOCUS       DASQGH010000492         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16298, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000492 DASQGH010000000
VERSION     DASQGH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16298"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..678
                     /locus_tag="VEW91_09675"
     CDS             115..678
                     /locus_tag="VEW91_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91887.1"
                     /translation="MRVIAVAIALAVLVGVPIAVRSLRAAPMSGTVNVAVSPGGQAVA
                     SGMSSLTFGYSPAVVTVKPGTTVVWTNKAVLPEPHFVTFLAPDPKTGEMQGGPPWLIV
                     RPKAGKEDSKNPLDMEFVENPLYVLPSELTGAPFRNSGYLWPERMGPPGAQSSWKTTF
                     TQQDAGKRLVYTCVIHPWMSGAVVVTK"
     gene            807..>2350
                     /locus_tag="VEW91_09680"
     CDS             807..>2350
                     /locus_tag="VEW91_09680"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HYM91888.1"
                     /translation="MAGRFRVFLAGIIFVLAAAGGLAADRIPGPHGLEGITPNNWTLT
                     PAGAQIPVGDRPLGAALSPDGHYLVVSNDGEGTQSLALVDTAGRSVVQTIPYRTPEAL
                     YVGVVWGPDGRRVYASAGGNNLVRTYEVRDGRLVETAPITLASQGARSYPAGLAITPD
                     GRTLLVAENLANRVRAVDLTTLRPGPAVPTGALPYAVAVTATGEKAYVSNWGDRTLSV
                     LRVAGLAPLATVAVGLHPGALAIDPVRPRLYAANTDDDSVSVVDTVSDRVAGTISLAP
                     YPGAPEGSVPDGLAVSADGRWLFVANAGNNDVAVVALGDPSAAAAAPRVVGLIPTAWY
                     PTTVTVSRDGRTLFVTNAKGLGAGPNPRGPSPVARRTAEIQYIASMIVGTVSVIPVPD
                     GPGLAQMTARVVRNNGFDETRNHLAQGARDASPQAIPRRVGDPSLIKHVIYIIKENRT
                     YDQVLGDLAAGNGDPSLVLFGRDVTPNHHRLAQEFVLLDNFYADAEVSADGHNWSTAA
                     MANDYV"
BASE COUNT          355 a          833 c          816 g          346 t
ORIGIN      
        1 aattagcaca ttccgcatga tgaccagcaa catgatcccc ttcgagcagg cgcttattga
       61 cggcgccgcg aacgcttaag caggacgcac tcaacaccga acggaggaag atcgatgcgg
      121 gttattgctg ttgcgatcgc cctggccgtg ctcgtaggtg tcccaatcgc ggtgcgctcg
      181 ctccgggccg cgcccatgtc gggcaccgtc aacgtcgcgg tcagtccggg cgggcaggcc
      241 gtggcgagcg ggatgtcgtc cctgacgttc gggtactcgc ccgcggtggt gaccgtgaag
      301 ccggggacga ccgtggtgtg gacgaacaag gcggtgctgc cggagccgca cttcgtgacg
      361 ttcctggcgc ccgacccgaa gacgggcgag atgcagggcg ggccgccgtg gttgatcgtt
      421 cgcccgaaag cgggcaagga ggactccaag aacccattgg acatggagtt tgtcgaaaac
      481 ccgctctacg tgctgccctc ggagctgacg ggcgcgccgt ttcggaatag tggctacctc
      541 tggccggagc gcatgggccc gcccggtgcg cagtcgagtt ggaagactac cttcacgcaa
      601 caagacgcgg ggaagaggct tgtctacacc tgcgtgatcc acccgtggat gtcgggcgcg
      661 gtggtggtca cgaaatagac gcgccgggga atgggcgcgc gaccggtggg agccccgttg
      721 ccgcccccgc tcccatcccc ggcgtcctgc tgagcggttt cgctcctcca cgatcctacc
      781 ccgcgtcgcc gacgggggaa tagccagtgg ccggacgctt ccgcgtgttc ctggcaggga
      841 tcatcttcgt cctggcagcc gcgggcggcc tcgcggcgga ccggatcccg ggaccccacg
      901 gcctcgaggg tatcaccccc aacaactgga cgctcacgcc cgccggcgcg cagatcccgg
      961 tcggcgaccg gccgcttggc gccgcgttga gcccggacgg tcactatctc gttgtcagca
     1021 acgacggcga ggggacccag tcgcttgcgc tcgtggacac cgcggggcgg tcggtggtgc
     1081 agacgatccc gtaccggacg cccgaggccc tgtacgtcgg ggtcgtctgg ggcccggacg
     1141 ggcgccgcgt gtacgcgtcc gcgggcggca acaacctggt gcggacgtac gaggtgcgcg
     1201 atggccggtt ggtggagacg gcgccgatca cgctggcgtc tcaaggggcg cgctcgtatc
     1261 cggcgggcct cgcgatcacc cccgacggcc gcacgctgct cgtcgccgag aacctggcca
     1321 atcgggtgcg ggcggtcgat ctcacgaccc tccgccccgg cccggcggtc ccgaccggcg
     1381 cgctgccgta cgccgtcgcg gtcaccgcga ccggcgagaa agcgtacgtc agcaactggg
     1441 gcgacaggac gctctcggtc ctgcgcgtcg ccggcctcgc cccgctcgcc accgtcgccg
     1501 tcggcctgca tccgggcgcg ctggccatcg acccggtccg gccccgcctc tacgcggcga
     1561 acaccgacga cgacagcgtc tcggtggtgg atacggtcag cgaccgcgtc gccggtacca
     1621 tctcgctggc cccctacccg ggggcccccg aggggagcgt tccggacggc ctcgcggtca
     1681 gcgcggatgg gcggtggctc ttcgtcgcca acgcggggaa caacgacgtc gcggtcgtcg
     1741 ccctggggga tccctcggct gcggcggccg cgccccgggt cgtcgggctg atccctaccg
     1801 catggtaccc gaccaccgtg accgtctcgc gcgacgggcg gacgctgttt gtcacgaacg
     1861 cgaagggatt gggcgccggt ccgaaccccc gcgggccgag ccccgtggcg cggcgcaccg
     1921 ccgagatcca gtacatcgcc agcatgatcg tggggacggt ctccgtcatc cccgtgcccg
     1981 acgggccggg actcgcccag atgacggccc gcgtcgtccg gaacaacggg ttcgacgaaa
     2041 cccgcaacca cctcgcccag ggcgctcggg acgcgtctcc gcaagcgatc ccccggcggg
     2101 tgggggaccc gtcgctcatc aagcacgtga tctacatcat caaggagaac cggacctacg
     2161 accaggtcct gggcgacctc gcggcgggga acggcgatcc atcgctcgtg ctcttcggcc
     2221 gggacgtcac ccccaaccat cacaggctcg cgcaggagtt cgtgctcctc gacaacttct
     2281 acgccgatgc cgaggtgagc gccgacggcc acaactggtc gacggccgcg atggccaacg
     2341 actacgtcca
//
LOCUS       DASQGH010000493         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50284, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000493 DASQGH010000000
VERSION     DASQGH010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50284"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VEW91_09685"
     CDS             <1..866
                     /locus_tag="VEW91_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYM91889.1"
                     /translation="LTIKTTAGVLGGSFSRVQFTPDLVPADLVGTRIYRPDQGTFDTE
                     LGPVFCNFLLADEINRAPAKVQSALLEAMQERQVTIGKETFLLPDPFLVLATQNPIES
                     EGTYPLPEAQVDRFMFKVVISYPSFHEEMTVVDRITEQSPSVSRVMDAQELLELQKIA
                     EAVYVDPRLHEYAVSLVTATRRPQEFGFGEAAKFISYGASPRGSLNLIIGAKALALLR
                     GREYAMPEDVRDLAPEVLRHRLLLSYEALAQDVTPEHLLQRVLEAVPVPKVHIGDPYG
                     DSRASLEAPRV"
     gene            847..1725
                     /locus_tag="VEW91_09690"
     CDS             847..1725
                     /locus_tag="VEW91_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYM91890.1"
                     /translation="MRRLEFKVVRRLDGFLFGDYTGVFYGPSLDLAEVRAYQPGDEVR
                     RIDWNVTARMNQIFVRQYLEEKELTAWLLVDLSPSMDFGTVQRFKRQIAVDFAGVAAY
                     IITRHGDKVGTIGFPHGAGGIFVPPRTGRLQALRIVHALTSTPPVSGGGRTDLAAVLD
                     RMGRFLRRRSLVFLVSDFHSPGGWEARLAELGRRHDVIAVRIEDPRERELPDVGGLYF
                     HDPETGHQVWVDTSDRNVRDRYHQLVAARDAELVQMMRRAAVDMLQLSTGSSLVEALV
                     KFVTFRRRRRWNSPGR"
     gene            1704..>2085
                     /locus_tag="VEW91_09695"
     CDS             1704..>2085
                     /locus_tag="VEW91_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HYM91891.1"
                     /translation="MEFTWPVMLWGLLLLPALVAWYGIGVRRARLRTVDRFADRPLFA
                     QLVTSLPRVWRHVSMALYFVSLALLLTAVGRPVMALPMPVNKAVVVLAVDTSGSMVAP
                     DIEPSRLEAAKIAARTFVDSFPQGP"
BASE COUNT          320 a          678 c          699 g          388 t
ORIGIN      
        1 cgctcacgat caagacgacc gcgggcgtgc tcggcggcag tttctcccgc gtccagttca
       61 cgcccgacct cgtccccgcc gacctcgtcg gtacgcgcat ctaccggccc gaccagggca
      121 ccttcgacac ggagctcggt ccggtcttct gcaacttcct gctcgcggac gagatcaacc
      181 gggcgccggc aaaggtccag tccgcgctgc tggaggcgat gcaggagcgc caggtgacga
      241 tcggcaagga gacgttcctc ctgccggatc cgttcctcgt gctggcgacg cagaacccaa
      301 ttgagagcga gggaacctat ccgctcccgg aagcacaggt cgaccggttc atgttcaagg
      361 tggtcatctc ctatccgtcg ttccacgagg agatgaccgt ggtcgatcgc atcactgagc
      421 agtcgcccag cgtgtctcgg gtcatggatg cgcaggagct tctggaactc cagaagatcg
      481 ccgaggcggt atacgtcgac ccccggctgc acgagtatgc ggtgagtctc gtgaccgcca
      541 cccggcgtcc ccaggagttc gggttcgggg aggcggccaa gttcatttcc tacggcgcga
      601 gcccgcgcgg ctccctgaat ctcatcattg gcgccaaagc cctcgcgctg ctgcgggggc
      661 gagagtatgc gatgccggaa gacgtccggg acctcgcccc ggaggtcctc cgccaccggt
      721 tgctcctgtc gtatgaagcg ttggcgcagg acgtcacgcc ggagcacctg ctgcagcggg
      781 tgctcgaagc ggtcccagtt cccaaggtgc acatcggaga tccctatgga gactcccgag
      841 cgagtcttga ggcgcctcga gtttaaggtc gttcggcgcc tggacgggtt tctgtttggc
      901 gactacaccg gggtgttcta tggccccagc ctggacctgg ccgaggtgcg ggcgtaccag
      961 ccaggggatg aagtccgccg gatcgattgg aacgtgacgg cgcgcatgaa ccagatcttc
     1021 gtgcgccagt acctcgagga gaaggagctc acggcatggc tcctcgtcga tctctccccg
     1081 tcgatggatt ttggcacggt gcagcggttc aagcgccaga tcgccgtcga tttcgcgggg
     1141 gtcgccgcct acatcatcac ccgtcatggc gacaaggttg gcacgattgg gttcccccat
     1201 ggggcaggcg ggatcttcgt cccgccgcgg accggccgcc tgcaggcgct ccgtatcgtc
     1261 catgcgctga catccacgcc ccccgtgtcc gggggggggc gcacggatct cgccgccgtg
     1321 ctggaccgga tggggcgctt cctccgacgc cgatcattgg tgttcctggt ttcggacttc
     1381 cactcgcccg gtgggtggga ggcgaggctg gccgagctcg gtcggcgcca cgatgtcatc
     1441 gccgtccgga tcgaagatcc gcgggaacgg gaactgcccg atgtgggcgg gctctacttt
     1501 catgatcccg aaacaggaca ccaggtgtgg gtcgacacat cggaccgcaa cgtgcgtgac
     1561 cggtatcacc agctcgtcgc cgcgcgcgat gccgagcttg tccagatgat gcgcagggcg
     1621 gcggtcgaca tgctgcagtt gtcgaccggg agctcgctcg tggaggcact ggtgaaattc
     1681 gtgacctttc ggaggcgccg ccggtggaat tcacctggcc ggtaatgctc tggggcctcc
     1741 tcctgctgcc ggccttggtg gcgtggtacg gcatcggggt ccggcgcgcc cgtctgcgga
     1801 cggtcgatcg gtttgccgac cggcccttgt ttgcgcagct cgtcacatcg ctaccacgag
     1861 tgtggcggca cgtcagcatg gccctgtact tcgtctcgct cgcgctgctc ctcaccgcgg
     1921 tcggccgccc ggtgatggcg ctcccgatgc cggtcaataa ggccgtggtc gtcctcgcgg
     1981 tagataccag cggcagcatg gtggcgcccg atatcgagcc gagccggctt gaggcggcaa
     2041 agatcgccgc ccggacgttc gtggatagtt tcccccaggg gccga
//
LOCUS       DASQGH010000494         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10544, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000494 DASQGH010000000
VERSION     DASQGH010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10544"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VEW91_09700"
     CDS             <1..733
                     /locus_tag="VEW91_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM91892.1"
                     /translation="KPDADMYNTHLNPFFTWHPTLDHIKFLFTQSHYPYWLWNTTWVA
                     VVSTLISVTVSLCAAYAIQRLRFRGSGTLGIAIFLTYLVPPIILFIPLAFIVFHLHLF
                     DSLWALVFTYPTFLIPFCTWLLMGYFKSIPHDLEESARIDGASRLAVVWRIILPLSLP
                     GIFSSTIFAFTLSWNEYLYALTFITSPEKLTVPVGSIIELVTGDVYHWGPLMAAALLG
                     SLPVALVYSFFVEHYVSALSGALKD"
     gene            736..1173
                     /locus_tag="VEW91_09705"
     CDS             736..1173
                     /locus_tag="VEW91_09705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYM91893.1"
                     /translation="MATRRRSARARRPSRPAIERIRLEGALPPPVSHYCDAVRAGDHL
                     YISGMVAMDRDGRVVGKGDPARQAEQIFENIARVLEHCGADVSRITSVLIHLTDMNDR
                     ATINPVRIRYFGEHRPASTLVQVARLVHPDLRVEVTCTAYLGR"
     gene            complement(1207..2778)
                     /locus_tag="VEW91_09710"
     CDS             complement(1207..2778)
                     /locus_tag="VEW91_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYM91894.1"
                     /translation="MPGRGHGKGPAQRREPRGPGVALSVAHILTLHLGTTACKASLFT
                     LGGEVAAQAATEYETVHPAPGWAEQDPEAWWEAALAGARRLPQDLRRTVCAVGLTSHR
                     GGVVPMDGQGRPLARCIIWMDRRSTAEVEALVRAFGRERLHEVTGLIPDTESTAGKLL
                     WLRSHAPEIFRAARLYLQPRDYLYFRLTGAPATDYTLASRTMLLDIGRRGWWDEGCAF
                     VGVTPGVFPPIYPSAAAPHQLGADAAGALGLPPGVPVALGAGDRPCGVLGSGAAAGWV
                     TVSTGTTTNVSAPVAGRPARVDRRVMCSLHAVDGMTVLEQGMSASGAILRWMRDRLLG
                     GRHDYRQLDALAADVPPGANGLQFLPFMMGARATRWNPAARGVWFGLTEAHGTGALAR
                     SILEGVAYELRACLDVLEEMDVPVSGILAVGGGARSTLWSRIFADVTGRPVRVPRQRD
                     AASLGAMLLAARAVGAIDRLEDAARSLNPVAATVVPDPGATRRYRELYVQYNRLYESL
                     RPIFDELAAAGPGEE"
     gene            complement(2709..>3002)
                     /locus_tag="VEW91_09715"
     CDS             complement(2709..>3002)
                     /locus_tag="VEW91_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM91895.1"
                     /translation="AILVNIARGPIVDQRALYRALVDRKIWAAGLDVFEVEPVPTDEP
                     LLRLDNVVIPPHLGSASTATRIRMATMAAENCLAGVTGKVPPNVVNPEAQESR"
BASE COUNT          437 a         1103 c         1016 g          446 t
ORIGIN      
        1 caagccggac gccgacatgt acaacacgca tctgaacccg ttcttcacct ggcatccgac
       61 gctggaccac atcaagttcc tgtttacgca gagccactac ccctactggc tgtggaacac
      121 gacctgggtg gcggtcgtct ccaccctgat ctcggtcacg gtcagcctgt gtgccgccta
      181 cgcgatccag cggttgcggt tccgcgggtc cgggacgctc ggcatcgcca tctttctcac
      241 ctatctcgtg cccccgatca tcctcttcat cccgctggcc ttcatcgtgt tccacctgca
      301 cctgttcgac agcctgtggg cgctcgtctt cacctatccg acgttcctga tcccgttctg
      361 cacctggctg ctgatgggct acttcaagtc gatcccgcac gatctggagg agagcgcccg
      421 catcgacggc gcgagccggc tggcggtcgt gtggcggatc atcctgcccc tttcgctacc
      481 ggggatcttc tcctcgacga tctttgcgtt cacgctgtcc tggaacgaat acctgtacgc
      541 gctgacgttc atcacctccc cggagaagct cacggtaccg gtgggatcga tcatcgagct
      601 cgtgaccggg gacgtctacc actggggacc gctgatggcc gcagcgctcc tgggctcgct
      661 cccggtcgcc ctcgtgtact cgttcttcgt cgagcactac gtctcggcgc tctcgggggc
      721 gttgaaggac taggggtggc gacccggcgc agaagtgcgc gagccaggcg gccgtccagg
      781 ccggcgatcg agcggatccg cctcgagggg gcgctgccgc cgccggtcag ccattactgc
      841 gacgcggtgc gcgccgggga ccatctctac atctccggca tggtcgcgat ggaccgggat
      901 gggcgcgtcg tcgggaaggg tgacccggcc cggcaggcgg agcagatctt cgagaacatc
      961 gcgcgggtgc tggagcactg cggcgcggac gtctcgcgga tcacgagcgt cctgatccac
     1021 ctcaccgaca tgaacgaccg ggccacgatc aacccggtcc gcatccgata cttcggcgag
     1081 caccggccgg cgagcaccct cgtccaggtc gcaaggctcg tccatcccga cctccgcgtg
     1141 gaggtcacct gcaccgccta cctcgggagg taacgcgaga ggccgcgccg gctccccgac
     1201 ccctgctcac tcttccccgg gacccgccgc cgcgagctcg tcaaagatgg ggcggagcga
     1261 ttcgtagagg cggttgtact ggacgtagag ctcccggtac cgtcgggtcg cgccgggatc
     1321 ggggacgacg gtggccgcca cagggttcaa cgaccgcgcg gcgtcctcaa ggcggtcgat
     1381 cgcgcccacc gccctggcgg ccagcagcat cgcgccgagg gaggccgcat cacgctggcg
     1441 cggcacgcgg accgggcgcc ccgtgacgtc ggcgaagatc cggctccaga gcgtgctgcg
     1501 ggccccgccg ccgaccgcca ggatcccgga gaccggcacg tccatctcct ccagcacgtc
     1561 gaggcaggcc cggagctcgt aggcgacccc ttccaggatg ctgcgggcga gggccccggt
     1621 cccgtgcgcc tcggtgaggc cgaaccagac cccgcgcgcc gccggattcc accgggtggc
     1681 ccgcgcgccc atcatgaacg gcaggaactg gagcccgttc gcgcccggcg gaacgtccgc
     1741 ggccagggca tcgagctggc ggtagtcgtg ccgccctccc agcagccggt cccgcatcca
     1801 gcggaggatc gcccccgaag cggacatccc ctgttccagc accgtcatgc catcgaccgc
     1861 gtggagcgag cacatcaccc gccgatccac ccgcgccgga cggccggcga ccggggccga
     1921 gacgttcgtc gtggtcccgg tcgagaccgt gacccatccc gcggcggcgc ccgagcccag
     1981 caccccgcag ggacggtcgc ccgcgcccag tgccacgggg acgccgggcg ggaggcccag
     2041 ggcccccgcc gcgtccgcgc cgagttgatg gggggcggcg gccgatgggt agatcggcgg
     2101 gaacacgccc ggggtcacgc ccacaaacgc gcacccctcg tcccaccacc cccgccggcc
     2161 gatgtcgagc agcatcgtcc tggaggccag ggtgtagtcg gtcgcgggag cacccgtcag
     2221 ccgaaagtac aggtagtccc ggggctgcag gtacaggcgg gccgcgcgaa agatttcagg
     2281 ggcgtgggat cgcagccaga ggagcttgcc ggcggtagac tccgtgtccg ggatcaggcc
     2341 ggtcacctcg tggagccgct cccgaccgaa cgcccggacc agcgcctcca cctcggcggt
     2401 ggaccgccgg tccatccaga tgatgcagcg ggcgaggggc cggccctgcc cgtccatcgg
     2461 gacgacaccg ccgcggtggc tggtgagccc caccgcgcac acggtccggc gcagatcctg
     2521 gggcaaccgg cgggcgccgg ccagcgcggc ctcccaccag gcctcggggt cctgctccgc
     2581 ccaccccggc gcggggtgca ccgtctcgta ctcggtggcc gcctgggctg cgacctcgcc
     2641 gcccagggtg aacagcgatg ccttgcatgc cgtggtcccg aggtggagcg tgaggatgtg
     2701 cgcgacgctc agcgcgactc ctgggcctcg gggttcacga cgttgggcgg gacctttccc
     2761 gtgaccccgg ccaggcagtt ttcggccgcc atcgtcgcca tccggatgcg cgtcgcggtg
     2821 ctggcgctgc cgaggtgcgg ggggatcacg acgttgtcga gccggagcag cggctcatcc
     2881 gtcggcacag gctcgacctc gaagacgtcc agcccggccg cccagatctt gcgatccacg
     2941 agcgcccggt agagcgcccg ctgatccacg atcggcccgc gcgcgatgtt cacgaggatc
     3001 gc
//
LOCUS       DASQGH010000495         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55761, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000495 DASQGH010000000
VERSION     DASQGH010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55761"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VEW91_09720"
     CDS             complement(<1..655)
                     /locus_tag="VEW91_09720"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYM91896.1"
                     /translation="MEIQRATTRELMDDPEGSVRDLRETLRDLARINRFLGAHAFVRR
                     YLDRALPVWRGRGHPGSRTLMILDVATGGADIPAAVVEWGRRRQVPVRIVGIDRHPAI
                     ARIAGTTSAACPAIAVMQADACALPFRDASFDVCLCHLGLHHLTRDQGIALLRRLHSL
                     ARVGFLVVDLLRSVGGYGGVWLVTRFARSPITRHDGPLSVRRALSWAEYRRLASETGV
                     "
     gene            820..1524
                     /locus_tag="VEW91_09725"
     CDS             820..1524
                     /locus_tag="VEW91_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM91897.1"
                     /translation="MPEETTVLVVDDDHKLIQLIRMYLEREGFGVLAAYDGPQALEMF
                     DRHRPGFVILDLMLPKIDGVSLCRQIRKNSNVPILMLTAKIEEVDKLIGLSAGADDYV
                     TKPFSPRELVARVRAILRRAAPSRPAASKLVQGGLTMDSERHEVFLDGREVRLTPIEY
                     KLLQALMEFAGRVFTRDQLLAHVYAFDEAVVVDRTIDVHIGKLREKLGDDPVSPRYIQ
                     TVRGVGYKFLENDRAG"
     gene            1514..2596
                     /locus_tag="VEW91_09730"
     CDS             1514..2596
                     /locus_tag="VEW91_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM91898.1"
                     /translation="MRGSLLWRLLGAQLLVIAIAVAASGFAISQQATHAFMAIMTRYH
                     VDPGTIAAEFQTASRRIFVVSSLVAATIAMLLGWALMTRIVRPLGQMMMLAERIAEGD
                     YARRVDIRGRDEIGRLAESLNQMAASLQRIEALRRDLVANVGHELRTPLTPLQGYLEA
                     LRDGVTPASPETLGILHEEVLRLVRLVDALHQLSQFDARVSRLRMGPLDLGLLLGRLV
                     AVYRPEFHRRGLHLTEAHDDALPSVEADGDLVSQALRNLIDNALRYAPAGATVTVAAH
                     LEDGGVRLAVSNTGEGIAPDDLPHVFERFYRGEKSRSRETGGAGIGLAIVREIAHAHG
                     GETGARSRDGLTTVWFSLPTRADAQG"
     gene            2583..3254
                     /locus_tag="VEW91_09735"
     CDS             2583..3254
                     /locus_tag="VEW91_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYM91899.1"
                     /translation="MRRVEAVIFDLDGLILDSETPDVLAWQAVYARYGMEFPIASWLQ
                     NVGRRDNPWDPIAPFRRPDSPAPPEAVAALWREQADALMAGYFKPLPGVVALLSALRH
                     RGMRTAVASSSRRAWILTVLARLGLEAEFDAVAGGDEVRRAKPAPDVYLLTARRLETA
                     PEACVALEDSPSGVRSARAAGIACIAVPSPLTRQLDFSAADLVVGSLEEVTPETILAL
                     GSAEA"
     gene            3282..>3731
                     /locus_tag="VEW91_09740"
     CDS             3282..>3731
                     /locus_tag="VEW91_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91900.1"
                     /translation="MRIATLLPSATEIVCALGLAGDLVGVTHECDFPPDVRRRPLVVR
                     GRIDAAQLSSVEINARVEASVVRGESLYDIDIQALRALRPDLLITQDLCEVCALPGTD
                     VDAAIDRLPTPPRVLRLHPHTLSDILEDIGAVGAATGRAAEAAGLVAD"
BASE COUNT          566 a         1307 c         1300 g          558 t
ORIGIN      
        1 gcactccggt ttcgctggcc agccggcgat attcggccca cgacagcgcc cggcggacgg
       61 agagcggtcc gtcgtggcgg gtgatcgggc tgcgcgcgaa gcgggtcacg agccacaccc
      121 cgccgtaccc cccgacgctc cgcagcaggt cgacgacgag gaagccgacg cgcgccaggc
      181 tatgcaggcg ccgcaggagc gctatcccct ggtcccgggt gaggtggtgc agccccagat
      241 ggcacaggca gacatcgaag ctcgcatcgc ggaacggaag cgcgcaggcg tcggcctgca
      301 ttaccgcaat cgccgggcag gctgccgacg ttgtgccggc gatccgcgcg atcgcagggt
      361 gccggtcgat gccgacgatc cgcacgggga cctgccgccg ccggccccac tcgaccaccg
      421 cggcggggat gtcggcgccc ccggtcgcga cgtcgaggat cattagcgtg cgggaaccgg
      481 ggtgtccccg cccccgccac acggggaggg cgcggtccag gtaccgccgg acaaacgcat
      541 gggcgcccag gaaccggttg atccgtgcaa ggtcccggag ggtctcgcgg aggtcacgca
      601 cggatccctc cggatcgtcc ataagttccc gcgtggtggc ccgctggatc tccatcgctt
      661 ccccgactct acccgcgcgg attcacgggc gcgtaacgtc ctcgcccggg tctctccgcc
      721 aacgtcccgg ggacgtgcat ggagggcttc atcaaatctt tatatgatat tcatacgcta
      781 cgagggccgc ggtagcaaga tcccagggag gcccgggtga tgcccgagga gacgaccgtt
      841 ctggtggtcg acgacgacca caagctcatc caactcatcc gcatgtacct cgagcgggag
      901 gggttcgggg tgctcgccgc ctacgacggc ccccaggcgc tggagatgtt cgaccgccac
      961 cgcccggggt tcgtcatcct tgacctgatg ctccccaaga tcgacggggt gtcactctgc
     1021 cggcagatcc ggaagaattc caacgtcccg atcctgatgc tcacggcaaa gatcgaggag
     1081 gtggacaagc tgatcgggct ctcggccggg gccgacgact atgtgaccaa gccgttcagc
     1141 ccccgggagc tcgtggcccg cgtgcgcgcc atcctccgcc gcgccgctcc gtcgaggccg
     1201 gccgcctcca aactcgtcca gggtggcctg acgatggata gcgaacggca cgaggtcttc
     1261 ctcgacgggc gggaggtgcg gctgaccccg atcgaataca agctgctgca ggcgttgatg
     1321 gagttcgccg ggcgggtgtt tacccgggac cagctcctgg cccacgtcta cgccttcgac
     1381 gaggcggtcg tggtggaccg gacgatcgac gtgcacatcg gcaagctgcg cgagaagctg
     1441 ggggacgacc cggtcagccc gcgctacatc cagaccgtgc gtggcgtggg gtacaagttc
     1501 ttggaaaacg accgtgcggg gtagcctcct gtggcgcctg ctcggtgcgc agctgctcgt
     1561 catcgccatc gccgtcgccg cctcggggtt cgcgatctcc caacaggcca cgcacgcctt
     1621 catggcgatc atgacgcgct accatgtcga tccggggacg atcgcggcgg agtttcagac
     1681 cgcgagccgc cggatctttg tggtcagcag cctggtcgcg gccaccatcg cgatgctcct
     1741 cgggtgggcg ttgatgacca ggatcgtgcg cccgctcggg cagatgatga tgctggccga
     1801 gcgcatcgcg gagggcgact acgcccggcg ggtcgatatt cggggacgcg acgagatcgg
     1861 gcggctggcg gagtcgctga accagatggc ggccagcctg caacggatcg aggcgctcag
     1921 acgggacctc gtcgccaacg tgggccacga gttacgcacg ccgctcaccc cgctgcaggg
     1981 gtacctggag gcgctccgag acggggtgac cccggccagc cccgaaaccc tcgggatcct
     2041 gcacgaggag gtgctgcggc tggttcggct cgtcgacgcc ctccaccagt tgagccagtt
     2101 cgacgccagg gtctcccggc ttcggatggg accgctggac ctgggcctgc tcctcggccg
     2161 gctggtcgcg gtctaccgcc cggagtttca ccgacgcggg ctccacctga ccgaggcgca
     2221 cgacgacgcg ctgccgagcg tcgaggcgga cggcgacctg gtgagccagg cgctccgcaa
     2281 cctgatcgac aacgccctca ggtatgcgcc cgcgggcgcc accgtcaccg tcgccgcgca
     2341 cctcgaggac ggaggcgtgc ggcttgcggt gtccaacacc ggtgagggga tcgcgccgga
     2401 tgacctcccg catgtcttcg aacggttcta ccgcggcgag aagtcccggt ctcgcgagac
     2461 gggcggcgcg gggatcgggc tcgcgatcgt ccgggagatc gcccacgcgc acgggggaga
     2521 gacgggtgcg cggagcaggg acggcctgac cacggtgtgg ttcagcctgc ccacccgtgc
     2581 cgatgcgcag ggttgaggcc gtcatcttcg atctcgacgg gctgatccta gacagcgaga
     2641 cccccgacgt tctggcctgg caggcggtct atgcccggta cggcatggag ttcccgatcg
     2701 cgtcgtggct ccagaacgtc gggcgaaggg acaacccatg ggatcccatc gcgccgttcc
     2761 gccggccgga cagccccgcc cctcccgaag ccgtcgccgc cctctggcgg gagcaggccg
     2821 atgccctgat ggccggctac ttcaaaccgc ttcccggcgt cgtcgccctg ctctcggcgt
     2881 tgaggcaccg cggcatgagg accgcggtgg cgtcgtcctc ccggcgcgcc tggatcttga
     2941 ccgtcctcgc ccggctcggc ctggaggccg agttcgatgc ggtcgccggc ggcgacgagg
     3001 tccggcgcgc gaagcccgcc cccgacgtct acctgctcac cgcccgccgc ctggaaacgg
     3061 cgccggaggc ctgcgtggcg ctcgaggaca gcccgagcgg cgtgcgctcg gccagggccg
     3121 ccgggatcgc ctgcatcgcg gttccctcgc cgctgacccg gcaactggac ttctccgcgg
     3181 ccgaccttgt ggtggggagc ctcgaagagg tcacgccgga gacgatcctg gcgctcgggt
     3241 cagcagaggc gtagcgccgc gcagtgtcga acccgggcgt gatgcggatc gcgaccctgc
     3301 tgcccagcgc aaccgagatc gtgtgcgcgc tcggactcgc cggtgacctc gtcggcgtca
     3361 cacacgagtg cgacttcccc ccggatgttc gccgccgccc gctggttgtc cggggaagga
     3421 tcgacgccgc acagctttcg agcgtcgaga tcaatgcgcg ggtggaagcg tcggtcgtac
     3481 ggggagagag tctctatgat atcgacatcc aggcgctccg cgccctccgc ccggacctgc
     3541 tgatcaccca ggacctctgc gaggtctgcg ccctcccggg aacggacgtc gacgccgcca
     3601 tcgacaggct gcccacgccc cccagggtat tgcggctcca cccccacacg ctctccgaca
     3661 tcctcgagga tatcggcgcg gtcggcgcgg caacgggccg cgcggcagag gccgcggggc
     3721 tcgtggcaga c
//
LOCUS       DASQGH010000496         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5038, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000496 DASQGH010000000
VERSION     DASQGH010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5038"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..156
                     /locus_tag="VEW91_09745"
     CDS             31..156
                     /locus_tag="VEW91_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYM91901.1"
                     /translation="MGAIKGARPPGVPETLIRLSIGLEDPADLIDDLDQALAAMG"
     gene            complement(153..722)
                     /locus_tag="VEW91_09750"
     CDS             complement(153..722)
                     /locus_tag="VEW91_09750"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HYM91902.1"
                     /translation="MRPAARPAPTRRRRPRWLWWLAGGAALAVAAGGAWWGLSSRSAA
                     QSGATIDGERLPDEGNKHVDIGLPIQYRDHPPASGNHYPQPAPAGVYPQGLAEGFWVH
                     SLEHGYIVLAYRPPASPEQLSQFDEMLRSFPRSKYRFVKLIVVPYKDMDHPYAALAWT
                     WRLWLDSFDREKVLAFYRAHVDRAPEDVP"
     gene            complement(906..1991)
                     /locus_tag="VEW91_09755"
     CDS             complement(906..1991)
                     /locus_tag="VEW91_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HYM91903.1"
                     /translation="MRRRARRLGGFLVGALLIGLATGPAPGAEKLRVGFVYVGPIGDA
                     GWTYAHDQGRKYLEAHDPGVQTTAVESVPEGADSERVFTDLARKGYKLVIGTSFGYMD
                     SMVKVAGAFPNVAFVHISGFKRAKNLATAFGRIEQPRYLTGLVAGSMTKSNIIGYVAA
                     HPIPEVVRGINAFTLGVRAVNPKAAVHVVWSNTWYDPAQEKEAAESLLSIGADVLTQH
                     QDSPAVVQAAAAKGKYAVGYNSDMHKFGPEAFLTAPVWNWGPMYVFLAKQVEAGTFKS
                     EDLWWGMDKGVVDIAPIGPMVPDAVKRLVAAKRSAILAGKSNEFAGPIKDQSGTVRVP
                     AGSALSDSAQLSMNWFVEGVVGKVPAK"
     gene            complement(2082..2948)
                     /locus_tag="VEW91_09760"
     CDS             complement(2082..2948)
                     /locus_tag="VEW91_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYM91904.1"
                     /translation="MTSLWHYAFVQHALLAGIITAVLAGCIGPIVTARNMAFAVHGLA
                     EVGFTGAAGAVLLGASPMIGVLTATFAAAAAIGGLGVRLRERDVAIGSVLAFGTGLGV
                     LFLALTTRYATEAFSILFGSILAVSEEDVIRSTVVGIIALLALAAIYRPLRFASVDPE
                     VAEARGVPIRLLSTVFLLLLALAVSEAVQIVGVLLILTLLIVPPAAAQRLTAHPGWAL
                     VCSVGIALVATLGGLTCALYTSLPVSFFVSAVSFTAYLLARALGPHLMNSAPAAGHPG
                     HPIGSIPDKLER"
     gene            complement(2945..>3581)
                     /locus_tag="VEW91_09765"
     CDS             complement(2945..>3581)
                     /locus_tag="VEW91_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM91905.1"
                     /translation="SGRVEVLGGPPRRGNAAIGYVPQQRAFDPDLPLRGRDLVRFGLD
                     GHRWGPTRGARGAGRQVDEMLALLDARPFADRPVGRLSGGEQQRLRVAQALIGGPQLL
                     LCDEPLLSLDLNYQQTIVTLLSRWNRTRGVTIVLATHDINPLLGAIDRVLLLAGGPWA
                     IGHADEVLTSETLTRLYRRPVDVLRLKDRIVVLGAELGGHEPLPDHPVGGR"
BASE COUNT          551 a         1267 c         1259 g          504 t
ORIGIN      
        1 tggggcgggt gggagagcct ggcgttcccc atgggggcga tcaagggcgc caggccgccg
       61 ggtgtgccgg agacgctgat ccgcctatcg atcggcctgg aggatcccgc ggatctgatc
      121 gacgatctcg atcaggcgct cgcggcgatg ggttagggga cgtcctcggg cgcgcggtcg
      181 acgtgggcgc ggtagaacgc cagcactttc tcgcggtcga acgaatcgag ccacagccgc
      241 caggtccatg ccagtgccgc gtacgggtgg tccatgtcct tgtacgggac gacgatcagc
      301 ttaacaaacc gatatttgct ccgagggaaa ctccggagca tctcatcaaa ctggctgagc
      361 tgctccggag acgccggcgg gcggtaggcc agcacgatgt acccgtgctc caggctgtgc
      421 acccagaacc cctcggccag gccctggggg tacacgcccg cgggggccgg ctgggggtag
      481 tggttccccg atgcgggggg gtggtcgcgg tactggatcg gtaggccgat gtcgacgtgc
      541 ttgttccctt cgtcgggcag ccgctcgcca tcgatcgtgg cgccgctctg ggcagccgag
      601 cgcgatgaga gcccccacca ggcgcccccg gccgcgaccg cgagggccgc gcctccggcg
      661 agccaccaga gccatcgggg tcgcctgcgg cgggtggggg ccggccgcgc ggccggccgc
      721 atcgttgcca cctggggccg cacctctgga ggcgccggcg gcgtgccctc gcgggctctt
      781 cgccgtcgct ccgcgcgcga aagttgtctt gtcaccgaga caccgacctc aaacgttcgc
      841 gcaggatgcc cgcgggtcct gttggacggc ccgccccgcg ggtcaatgga ccggccactt
      901 ggaggctatt tggcgggcac cttcccgacc acgccctcga cgaaccagtt catcgacagt
      961 tgcgcgctgt cgctgagggc gctccccgcg gggacccgca ccgtcccgct ctgatccttg
     1021 atcgggccgg caaattcgtt cgacttcccc gcgaggatcg ccgaccgctt ggcggcgacc
     1081 aggcgcttga ccgcatccgg gaccatcggc ccgatcggcg cgatgtccac cacccctttg
     1141 tccatgcccc accagaggtc ctcgctcttg aaggtccccg cctcgacctg cttggccagg
     1201 aacacgtaca tcggccccca gttccacacc ggggcggtga ggaaggcctc cgggccgaac
     1261 ttgtgcatgt cggagttgta ccccaccgcg tacttgccct tggcggcggc cgcctggacc
     1321 accgccggcg agtcctggtg ctgcgtcagg acgtccgcgc cgatgctaag caggctctcc
     1381 gccgcctctt tctcctgcgc ggggtcgtac caggtgttgg accagaccac gtggacggcg
     1441 gccttggggt tgaccgcccg gaccccaagg gtgaacgcgt tgatcccgcg gaccacctcg
     1501 gggatggggt gcgccgccac gtacccgatg atgttcgact tggtcatcga gcccgccacc
     1561 aacccggtca ggtagcgcgg ctgttcgatc cgcccgaacg cggtcgccag gttcttcgcc
     1621 cgcttgaacc ccgagatatg gacgaaggcg acgttgggga acgcgccggc gaccttgacc
     1681 atcgaatcca tgtagccaaa cgaggtcccg atgacgagtt tgtatccctt gcgggccagg
     1741 tcggtgaaga cgcgctcgga gtcggcgccc tcgggcacgc tctccaccgc ggtcgtctgg
     1801 accccgggat cgtgggcctc gaggtacttg cggccctggt cgtgcgcgta cgtccacccg
     1861 gcatcgccga tgggccccac gtagacgaac ccaacccgga gcttctccgc gccgggggcc
     1921 ggcccggtcg ccagcccgat aaggagcgcc cccacgagga acccgcccag ccgccgcgct
     1981 cgcctgcgca tgcccacccc tccgtgaccc tgatcgtctg gtgccgcttc cctcgagtct
     2041 tcgcgccgga cgggcgcgcc ccttccgcgc cccctccagg atcagcgctc caacttgtcg
     2101 ggaatcgacc cgattgggtg gccggggtga cccgccgccg gcgcgctatt catgaggtgc
     2161 gggccgaggg cgcgcgcgag gaggtacgcg gtgaagctca cagccgagac gaaaaagctg
     2221 accgggaggg aggtatagag ggcgcaggtg aggccgccga gggtggcgac gagcgcgatc
     2281 ccgacgctgc acacgagggc ccacccggga tgcgcggtga gccgctgggc ggctgccggg
     2341 ggaacgatca gcagggtgag gatcaacagc acgcccacga tctggacggc ctccgagacg
     2401 gcgagggcga gcagcagcaa gaacaccgtg gagagcaggc ggatagggac gccgcgggcc
     2461 tcggccacct cggggtcgac gctggcgaac cgcagggggc ggtagatcgc tgccagcgcc
     2521 agcagcgcga tgatgccaac gaccgtggac cgtatcacat cctcctcgct gaccgcgagg
     2581 atcgaaccga agaggatgct gaacgcctcc gtcgcgtacc gcgtggtgag ggccaagaac
     2641 aagacgccga gcccggtgcc aaacgcgagc acgctcccga tggcgacgtc gcgttcgcgc
     2701 agccgcacgc cgaggccgcc gatggccgcc gcggccgcga acgtcgccgt cagcacgccg
     2761 atcatgggcg acgcgccgag cagcacggcg cccgccgctc cggtgaagcc cacctccgcc
     2821 aggccgtgga cggcaaaggc catgttccgg gcggtgacga tcgggccgat gcagcccgcg
     2881 agcaccgcgg tgatgatccc ggcgagcagc gcgtgctgca cgaaggcgta gtgccagagc
     2941 gacgtcatcg tccccccacg gggtggtcgg gcagcggttc gtggccgccc agttccgcgc
     3001 ccaggaccac gatccggtcc ttgaggcgga ggacatccac gggcctgcgg tacaggcgcg
     3061 tcagcgtctc ggaggtgagc acctcgtcgg cgtggccgat cgcccacggg ccgccggcca
     3121 gcagcaggac gcggtcgatc gcacccagca gcggattgat atcgtgcgtc gcaaggacga
     3181 tggtgacgcc gcgcgtgcgg ttccaccgcg acagcagcgt gacgatcgtc tgctggtagt
     3241 tcaggtcgag gctgagcagc ggctcatcgc agagcaggag ttgcggcccg ccgatcagcg
     3301 cctgggcgac gcgaaggcgc tgctgttcgc cccccgacag ccgcccgacc ggccggtcgg
     3361 cgaacggccg cgcgtcgagc agcgccagca tctcgtcgac ctggcgcccc gcaccccgcg
     3421 cgcctcgggt ggggccccac cggtggccgt ccaagccaaa ccggacaaga tcccggcccc
     3481 ggagcggaag atcgggatca aacgctcgct gctggggcac gtacccgatg gccgcgttgc
     3541 cccgacgggg cgggcccccc aggacctcca cccggccgct g
//
LOCUS       DASQGH010000497         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30622, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000497 DASQGH010000000
VERSION     DASQGH010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30622"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VEW91_09770"
     CDS             <1..428
                     /locus_tag="VEW91_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91906.1"
                     /translation="AGPTDVLTVNVGSITAGQNTWFIIQGGGYVQLNVASQLTVGQGS
                     YFGVDGSGNLLPASHLVVRSCNTGAPSPAVWFNQTGKLSAVIIAPYGNVQMDQAQNSQ
                     GAVLANNIQFDQGTTLAFDASASAVGFGFNKLLSWQDVP"
     gene            complement(425..904)
                     /gene="ruvX"
                     /locus_tag="VEW91_09775"
     CDS             complement(425..904)
                     /gene="ruvX"
                     /locus_tag="VEW91_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYM91907.1"
                     /translation="MSRFLGLDLGTRRIGVALSDPTGTVATPLLVLAHTDRRRDLAAV
                     ARLAAAHGVARIVVGWPRNMDGTRGPAARRAEAFADALRRLVDVPIDLWDERLSTVAA
                     DRTLREVGVRRDRRRVVRDQIAAALILQAYLTARAPAGESNDPPGSPRQRAGGRAWR"
     gene            complement(901..3576)
                     /gene="alaS"
                     /locus_tag="VEW91_09780"
     CDS             complement(901..3576)
                     /gene="alaS"
                     /locus_tag="VEW91_09780"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYM91908.1"
                     /translation="MRRADEIREAFLRYFEGQGHTRVPSASLVPAGDPTLLFTNAGMV
                     QFKDVFLGLEQRSYRRAVTVQKCMRVSGKHNDLENVGPSPRHHTFFEMLGNFSFGDYF
                     KRDAVRFAWELTTGTLGIPPDRLVQSVLDGDDEALTAWTALGIPRGRIVRMGEATNFW
                     MMGDVGPCGPTSELHYDWGAAACTCGRPDCSVALDNGCARWLEIWNLVFMQFDQAPDG
                     TRTPLPQPGVDTGMGLERIVSVIQGVPSNYDTDLFLPLFGHIQARLKHSDAQRAQHMV
                     AYRVLADHGRAMTFLVADGVVPDNEGRGYVLRMIMRRAIRFARRAGARAPVLAEVGEA
                     VVATMRAAYPDLVTHLPFIREVLGAEEERFHQTLDAGLGRLDGLIAESRHRGLTVLPG
                     ADVFRLYDTFGFPPDLTRDVAREHGLEIDEAGFAAEKARQQERSREAAASQSDARERA
                     AYAALREGGTPTEFVGYETLEADGRILALLRAGDLVGTAAAGDQVEVVCDRTPFYAEA
                     GGQVGDTGKIQTPGGGVAVTDTQRPVLGLVVHRGQVAGGPVRVGEPARLVVDASRRRD
                     IMRNHTATHLLHRALREILGEHARQAGSLVAPDRLRFDFLHLRPVSTDERARLEARVN
                     DQVLAALPVRTEVMAYRDAVASGAVALFGEKYGDTVRVVTIDDYSRELCGGTHVRSTA
                     EIGLFMITAESSVGSGTRRIEALTGRAAVGRARESEAVLREAAGALRVPVAEISDRVR
                     QLADRARARASEVASAQAGAAGPDLEAAVRAAPEVDGVTVVGIVTSGGDQAALRALGD
                     RVKPRLGSGVIVAASSANGRIEVVVMATRGAIARGAEAGKVLAALNRRLGTRGGGRPE
                     LAQGGGGDPALLDAVMADLPAVVREALGAPSGKGR"
     gene            complement(3573..>3952)
                     /locus_tag="VEW91_09785"
     CDS             complement(3573..>3952)
                     /locus_tag="VEW91_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM91909.1"
                     /translation="AALATLDVLVEERLPARAEPLGAQFLGRLNEIRVQHPGVVSAVR
                     GRGLMLGVEFAHRDYALMTSAEAGRRGVITFYTLNNPSVIRIEPPLVITPELVDRACE
                     GIAGAVAGAARLLAGVREASAGS"
BASE COUNT          571 a         1504 c         1337 g          540 t
ORIGIN      
        1 ccgccgggcc gaccgatgtc ctcacggtca acgtgggctc gatcacggcc ggccagaaca
       61 cctggttcat cattcaaggg ggaggctacg tccagctgaa cgtggcgagc cagctgaccg
      121 tcgggcaggg aagctacttc ggcgtggatg gctcgggcaa tctgctgccg gccagccacc
      181 tcgtcgtgcg cagctgcaac accggcgcgc cgtccccggc ggtctggttc aaccagaccg
      241 ggaaattgag cgcggtgatc atcgctccgt acggcaacgt ccagatggac caggcgcaga
      301 actcgcaggg cgcggtcctg gccaacaaca tccagttcga tcagggcaca accctggcgt
      361 tcgacgcctc cgccagcgcg gtcgggttcg gcttcaacaa gctcctctcc tggcaggacg
      421 tcccctagcg ccaggctcgg cctccggccc tctgccgcgg tgaccccggc gggtcgttgg
      481 actccccggc cggggcgcgg gcagtgaggt aggcctggag gatcaacgcc gcggcgatct
      541 ggtcccgcac gacgcgccgc cggtcccgcc tcacaccgac ctcgcgtaac gtccgatccg
      601 ccgcgacggt cgagagccgc tcgtcccaca gatcgatggg cacgtccacg agccgacgca
      661 gcgcatcggc aaaggcctcc gcgcgcctgg ccgcagggcc gcgcgttccg tccatgttcc
      721 ggggccaccc cacgacgatg cgggcgacgc cgtgggcggc ggccagtctg gccaccgcgg
      781 cgaggtcacg cctgcggtcc gtgtgggcaa gcaccaggag cggggtcgcg accgtgccgg
      841 tggggtcgct gagcgccacc ccgatgcggc gcgttccgag gtccagaccc aggaatcgac
      901 tcaccggccc ttgccggatg gggcgccgag cgcttcgcga accaccgcgg gaagatccgc
      961 catcaccgcg tcgagcaacg ccggatcacc cccgcctccc tgggccagtt cgggccgccc
     1021 tcccccgcgg gtgcccagcc ggcggttgag cgccgccagg acctttccgg cctcggcccc
     1081 gcgtgcgatc gcgccgcgtg tcgccatcac cacgacctcg atccggccgt tggcagatga
     1141 ggcggccacg atgacccccg acccgagccg gggcttgacg cggtccccca gcgcgcgcag
     1201 cgccgcctga tcgccgcccg aggtgacaat ccccacgacc gtgacgccgt ccacctcggg
     1261 ggcggcgcgc acggctgcct caaggtccgg tcccgcggcg cccgcctggg cggaggcgac
     1321 ctcggatgcg cgcgcgcggg cccggtcagc cagctgcctg acccggtcgg agatctccgc
     1381 gaccggtacc cggagcgcgc cggcggcttc ccggaggacc gcctcgctct cccgcgcgcg
     1441 gcccaccgcg gcccggcccg taagcgcctc gatccgccgg gtcccgctgc cgaccgagga
     1501 ctccgcggta atcatgaaca ggccgatctc agcggtggac cggacgtggg tcccgccgca
     1561 cagctcgcgg ctgtagtcgt cgatcgtgac cacgcgcacc gtgtccccgt acttctcgcc
     1621 gaacagcgcc acggccccgg acgccacggc gtcccggtag gccatcacct ccgtgcgcac
     1681 gggcagggct gcgagcacct ggtcattcac ccgggcctcg agccgggcgc gctcgtccgt
     1741 ggagacgggg cggaggtgta ggaagtcgaa ccgcagccgg tccggggcca cgagcgatcc
     1801 tgcctgccgc gcgtgctcgc ccaggatctc ccggagggcg cggtgcagga gatgggtcgc
     1861 ggtgtggttc cgcatgatgt cgcgccggcg cgaggcatcc acgacgaggc gcgcgggctc
     1921 ccccaccctc acggggcccc cggccacctg tccgcggtgg accacgaggc ccagaacggg
     1981 gcgctgggtg tcggtcaccg ccaccccgcc gccaggggtc tggatcttcc cggtgtcccc
     2041 gacctgcccg ccggcctccg cgtagaacgg cgtccggtcg cagacgacct ccacctggtc
     2101 gcccgcggcg gccgttccca ccaggtcgcc ggcgcggagc agggcgagaa tgcggccgtc
     2161 ggcctccaac gtctcgtagc cgacgaactc ggtcggcgtt cccccttcgc gcagggccgc
     2221 atacgccgcg cgctccctgg cgtcggattg ggaggctgcc gcttcgcggg agcgctcctg
     2281 ctggcgggcc ttctccgctg cgaatcccgc ttcatcgatc tcgagcccgt gttcccgcgc
     2341 gacgtcgcgg gtgagatccg gcggaaaccc aaaggtgtcg tacagccgaa acacgtccgc
     2401 cccggggagc acggtgagcc cgcgatgccg gctctccgcg atcaggccgt ccagccggcc
     2461 gagcccggcg tcgagcgtct ggtggaaccg ctcttcctcg gccccgagca cctcccggat
     2521 gaacggcaga tgggtgacga ggtcgggata ggccgcgcgc atcgtcgcaa caacagcctc
     2581 gcccacctcc gccagcaccg gggcccgcgc accggcgcgg cgcgcaaacc ggatcgcccg
     2641 ccgcatgatc atcctcagca catacccgcg cccttcgttg tcgggcacca ccccgtcggc
     2701 gacgaggaac gtcatcgcgc ggccgtgatc cgccagcacc cggtacgcca ccatgtgctg
     2761 cgcgcgctgc gcgtcggaat gtttgagccg cgcctggatg tggccgaaca ggggcagaaa
     2821 caggtctgtg tcgtagttgc tcggaacacc ctggatcacc gagacgatcc gctcgaggcc
     2881 catcccggta tccacccccg gctggggcaa cggggtccgg gtgccgtcgg gggcttgatc
     2941 gaactgcatg aacaccaggt tccagatctc gagccaccgg gcgcacccgt tgtccagcgc
     3001 gacgctgcag tccgggcggc cgcaggtgca cgccgccgcc ccccagtcgt aatgcagttc
     3061 gctcgtgggc ccgcaggggc cgacatcccc catcatccag aagttcgtcg cttcccccat
     3121 ccgcacgatg cggccccggg ggatgccgag ggccgtccat gcggtcagcg cttcgtcgtc
     3181 gccgtccagc accgactgca ccaaccggtc gggcggaatc ccgagcgtcc cggtggtcag
     3241 ctcccaggca aaccgaaccg cgtcccgttt gaagtaatcc ccgaagctga agttcccaag
     3301 catctcgaag aaggtgtggt ggcgggggga cgggccgacg ttctccagat cgttgtgctt
     3361 gccgctgacc cgcatgcact tctgcaccgt gaccgcgcgc cggtacgacc gctgctccag
     3421 gccgaggaac acgtccttga actggaccat cccggcgttc gtgaacagga gcgtcggatc
     3481 tcccgccggc accagcgagg cgctgggcac ccgggtgtgg ccctgacctt cgaagtaccg
     3541 gaggaacgcc tctcggatct cgtcggcccg cctcacgacc ccgcagaggc ctcccgcacc
     3601 ccagccagca ggcgtgcggc gcccgccacg gcgcccgcga tcccctcgca ggcgcggtcg
     3661 accagctcgg gcgtgatcac cagcgggggt tcgatccgga tgacgctcgg gttgttcagg
     3721 gtgtagaagg tgatgacccc ccggcgcccg gcctcggccg aggtcatgag ggcatagtcg
     3781 cggtgcgcaa actcgacccc gagcatcagc ccccggcccc gcaccgccga caccaccccc
     3841 gggtgctgta cacggatctc gttgaggcgc ccgaggaact gcgccccgag cggctcggcg
     3901 cgtgcgggga ggcgttcctc caccaggacg tcgagcgtgg ccagcgccgc gc
//
LOCUS       DASQGH010000498         5821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33393, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000498 DASQGH010000000
VERSION     DASQGH010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33393"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VEW91_09790"
     CDS             complement(<1..185)
                     /locus_tag="VEW91_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012152252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYM91910.1"
                     /translation="MVHLTIDGQAVAVPKGTTVWQAARQAGIEIPIFCYHDRMPPLGA
                     CRMCLVEIEKMPKLVTS"
     gene            complement(203..1471)
                     /gene="nuoF"
                     /locus_tag="VEW91_09795"
     CDS             complement(203..1471)
                     /gene="nuoF"
                     /locus_tag="VEW91_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HYM91911.1"
                     /translation="MPRPLLTKFLGRPGRERIDAYLETGGYQAAATALRELAPDAIVD
                     LVEASGLRGRGGAGFPAGRKWKLTPKREGEARYLVVNADEGEPGTFKDRTLIEQDPHA
                     LLEGILIAAFANRVPRAFIYLRGEFFTGYDRLRSAVGEAYARGYFGRNILGSGFDLEV
                     VIHRGAGAYICGEETALLESLEGKRGFPRLKPPFYPAVKGLYGQPTVLNNVETLAHIP
                     HIIALGVDGYRAYGPPVLYSVSGHVVSPGVKELPIGTPLREIIFEHAGGLRPGRTVKA
                     VFPGGSSSAILTAEHLDTPADFEPLAKLGSMLGSSAIIVMDETTCIPAVIRRTVEFYR
                     DESCGKCTPCREGTIWLTQILDRIVAGQGRMEDLDLLDGIARNMTGTCFCPLGESVPP
                     SLRASLRYFRGEYEHHIRTGACDVRPVVAQ"
     gene            complement(1473..1946)
                     /gene="nuoE"
                     /locus_tag="VEW91_09800"
     CDS             complement(1473..1946)
                     /gene="nuoE"
                     /locus_tag="VEW91_09800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HYM91912.1"
                     /translation="MVPPLSQAVLAEIARLRALYPEPRSALLPALHAAQAETGYLTEG
                     ALEGVGQALGLPLTEIVSVATFYEMFHLEPLGRHHIRICTNLSCYLNGCDRVLERLCA
                     RLGVRPGETTADGRVTVEAVQCLAACEEAPVLLVDAERRPRMTPDAVDDLLAGLA"
     gene            complement(1946..3124)
                     /gene="nuoD"
                     /locus_tag="VEW91_09805"
     CDS             complement(1946..3124)
                     /gene="nuoD"
                     /locus_tag="VEW91_09805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HYM91913.1"
                     /translation="MTERTAETLTLNMGPQHPSTHGVLRLVLELEGEIVRKCSPVVGY
                     LHTGIEKEMETRTYLQNVTLVDRIEYVASYMEEMAFYASVEALMGLAVPPRARYIRVL
                     MCELNRIASHLIYLGTAALDLNISSVFMYCFADRERCLDLSEMVSGQRMMPGYFRAGG
                     VAADLPDEFVPAARAFLDEIPRRVDEYARLLDDNLIWRERTQGVAVLSRGDAIALGAT
                     GPVLRGSGVPHDIRKLLPYGGYDEFEFDVPTAAQGDAYARYRVRLEEMRQSRRIALQA
                     LERLPAGPVATSDRKVTLPPRAELARSMEAVIHQFKLVSEGYHPPAGDSYVATESARG
                     EKGYYIVSDGSNRPVRVHVRAPSFYNLQTLPAMIEGRPLADAVVAIASLDIILGDVDR
                     "
     gene            complement(3121..3681)
                     /locus_tag="VEW91_09810"
     CDS             complement(3121..3681)
                     /locus_tag="VEW91_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYM91914.1"
                     /translation="MGQAPILDRLRPHVPWGLTTPKEERGRAILLATPGVLLEVLQAA
                     RQHLALDSLVDLTCWDRLPASPRFEVVYLLGQAAGPDRLTVKVQIDGDPPRLSSAVGI
                     YPGAAWPEREVYDMFGVAFTGHPDLRRILMPDDWEGHPLRRDFPLTEEAVEFKGHTPK
                     VPSQIIPFFRPHSPSGSGGEPPPGSP"
     gene            complement(3696..4187)
                     /locus_tag="VEW91_09815"
     CDS             complement(3696..4187)
                     /locus_tag="VEW91_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYM91915.1"
                     /translation="MGVIASERDLERNVLTTTLDRIVAWARKSSLWPVQFGLACCAIE
                     MMSTAAPRFDIARFGAELFRASPRQSDLMIVAGRVSQKMAPVVRHIYDQMLEPKWVIA
                     MGDCASTGGLYNNYAVVQGVDKILPVDVYVAGCPPRPEALLDGLRLLQDAIAQGSPAR
                     RAP"
     gene            complement(4178..4513)
                     /locus_tag="VEW91_09820"
     CDS             complement(4178..4513)
                     /locus_tag="VEW91_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYM91916.1"
                     /translation="MLVHLILAVLLAVTLLGLHRLAGGRRPTLEKSLPYESGVWPIGS
                     ARERVPIRYYLVAMLFILFDIEIVFIYPWAVLVRELGPAGLVEMFAFLAVLGGGYLYL
                     LRRRALEWE"
     gene            complement(4624..5478)
                     /locus_tag="VEW91_09825"
     CDS             complement(4624..5478)
                     /locus_tag="VEW91_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM91917.1"
                     /translation="MTADLVAIDVHVHPTSPEAIGGFGSDLEAMGEYFGRSIPSVSID
                     KLADQYRAQRMIAVQLAKASSTTSGRPPVPNDHIASAVRAHPDVFIGFAGVDPWKGRL
                     AVDEARRAHEMLGLRGLKLHPGLQKFAANDERFYPLWATAAELGLVCLFHSGTMATGA
                     GLPGGAGYKLKYVNPMLLDDVAADFPSLQIILAHPSWPWQAEQLAIARHKSNVYLDLS
                     GWSPKYFPPELVQHVNSVLQDKALFGSDWPFMTPERWLADFAALPIKDAVRPKILRDN
                     ARRLLGVA"
     gene            complement(5535..>5821)
                     /locus_tag="VEW91_09830"
     CDS             complement(5535..>5821)
                     /locus_tag="VEW91_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91918.1"
                     /translation="SGPFVAYSPRRAEAYVYRKTARGSFQTAMEGLPEGRGTIASRFA
                     TPGEPGVMYAANNHGVFLTPDAGRSWTALEIPWPNGAFAQGVEAQICLPD"
BASE COUNT          928 a         2052 c         1979 g          862 t
ORIGIN      
        1 cacgaggtca cgagcttggg catcttctcg atctcgacca ggcacatccg gcacgcgccg
       61 agggggggca tccggtcgtg gtagcagaag atcgggatct cgatcccggc ctgccgcgcc
      121 gcctgccaca cggtcgtccc cttggggacg gcgaccgcct ggccgtcgat ggtgaggtgg
      181 accatctgca ccttttgctc gctcactgcg ccaccaccgg gcgcacatcg cacgcgccgg
      241 tcctgatgtg gtgctcgtac tccccgcgga agtaccggag ggacgcccgg aggctcgggg
      301 gcacgctctc gccgagcggg cagaagcacg tgccggtcat gttccgggcg attccatcca
      361 ggaggtcgag gtcctccatg cggccctggc ccgccacgat ccggtcgagg atctgggtca
      421 gccagatcgt gccctcgcgg cagggcgtgc acttcccgca cgactcatcc cggtagaact
      481 cgaccgtgcg ccggatcacc gccgggatac aggtcgtctc gtccatcacg atgatcgcgc
      541 tcgagccgag catcgacccc agcttggcca gcggctcgaa gtccgccggg gtgtcgaggt
      601 gctcggcggt gaggatcgcc gaggacgacc ccccggggaa caccgccttc acggttcgcc
      661 cgggccgcag cccgcccgca tgctcgaaga tgatctcccg gagcggggtg ccgatcggca
      721 gttccttgac cccggggctc accacgtgcc ccgagaccga gtagagcacc ggcggaccgt
      781 aggcgcggta tccgtccacg ccgagggcga tgatgtgcgg gatgtgcgcg agggtctcca
      841 cgttgttcag cacggtcggt tggccgtaca gtcctttgac cgccgggtag aagggcggct
      901 tcagccgcgg gaacccccgc ttcccttcca ggctctcgag gagggccgtc tcctccccgc
      961 agatgtacgc cccggccccg cggtggatca cgacctccag atcgaacccc gaccccagga
     1021 tgttgcgccc gaagtagccg cgggcgtagg cctcgccgac cgcgctcctc agccggtcgt
     1081 acccggtgaa gaactccccc cgcaggtaga tgaacgcccg gggcacccgg ttcgcgaacg
     1141 cggcgatcag gatcccctcg agcagcgcgt gggggtcctg ctcgatgagg gtccggtcct
     1201 tgaacgtccc cggctcgccc tcgtcggcgt tcacgacgag gtaccgggcc tccccctcgc
     1261 gcttcggcgt gagcttccac ttccggccgg cggggaaccc ggcgccgccc cgcccgcgca
     1321 gccccgaggc ctcgaccaga tcgacgatcg catcgggcgc gagctcgcgc agcgcagtgg
     1381 cggcggcctg gtatcccccg gtctccaggt acgcgtcgat ccgctcgcgg ccgggccggc
     1441 cgaggaactt ggtcaggagc gggcgcggca tctcaggcca gccccgcgag caggtcgtcg
     1501 accgcgtccg gggtcatccg ggggcgccgc tccgcatcga ccaggaggac cggagcctcc
     1561 tcacacgcgg ccaggcactg caccgcctcc acggtcaccc ggccgtccgc cgtggtctcg
     1621 ccggggcgca ccccgaggcg ggcgcacagg cgctccagca cccggtcgca cccattgagg
     1681 tagcaggaca ggttggtgca gatgcggata tggtgccggc cgagcggctc caggtggaac
     1741 atctcgtaga aggtggcgac cgacacgatc tcggtgagcg gcaggccgag ggcctggccg
     1801 acgccctcca gcgcgccctc ggtcaggtac cccgtctcgg cctgcgcggc gtgcagcgcc
     1861 ggcagcagcg ccgaccgggg ctcgggatac agcgcgcgca gccgcgcgat ttccgcgagg
     1921 accgcctgcg agagtggggg aaccatcagc ggtcgacatc ccccaggatg atgtcgaggc
     1981 tcgcgatggc gacgacggcg tcggcgaggg gccgcccttc gatcatcgcc gggagggtct
     2041 gcaggttata gaacgacggc gcgcgcacgt gcacccggac cggccggttg ctcccatcgc
     2101 tgacgatgta atagcctttc tcgccccgcg ccgactcggt ggccacatag ctgtccccgg
     2161 ccggcgggtg gtagccctcg ctgaccagct tgaactggtg gatcacggcc tccatgctcc
     2221 gggccagctc cgctcggggg ggcagcgtga ccttgcggtc gctcgtggcg accgggccgg
     2281 ccgggaggcg ctccagggcc tggagcgcga tccgccggct ctgccgcatc tcctccaggc
     2341 gcacgcggta gcgggcgtag gcgtcgccct gggcggcggt cggcacatcg aactcgaact
     2401 cgtcgtaccc cccgtacggc aggagcttgc ggatgtcgtg gggcaccccg gacccccgga
     2461 gcaccggacc cgtggccccg agcgcgatcg catccccgcg cgagaggacc gcgaccccct
     2521 gggtgcgctc gcgccagatc aggttgtcgt ccagcaaccg cgcatattcg tcgacccggc
     2581 gcggaatctc gtccaggaac gcgcgcgcgg cggggacaaa ctcgtccggg aggtcggcgg
     2641 cgaccccgcc cgcccgaaag tatcccggca tcatccgctg gcccgacacc atctccgaga
     2701 ggtcgaggca ccgctcccgg tccgcaaagc agtacatgaa cacgctgctg atgttcaggt
     2761 cgagcgccgc ggtgccgaga tagatcaggt ggctcgcgat gcggttcaac tcgcacatca
     2821 ggacccggat gtagcgggcg cggggcggca ccgcgagccc catcagggcc tcgaccgacg
     2881 cgtagaacgc catctcctcc atgtagctgg ccacgtactc gatccggtcg acgagggtga
     2941 cgttctgcag gtaggtgcgg gtctccatct ccttttcgat cccggtgtgc aggtacccca
     3001 ccaccggcga gcacttccgc acgatctcgc cctcgagctc gagcaccaac cgcagcacgc
     3061 cgtgggtgct ggggtgctgc ggccccatgt tgagggtcag ggtctcggct gtgcgctcgg
     3121 tcacggggat cccggcggcg gctcgccgcc cgatccggac gggctgtgcg gccggaagaa
     3181 cgggatgatc tggctcggca ccttgggggt gtggcctttg aactcgaccg cctcttcggt
     3241 gagcgggaag tcccgccgca gggggtggcc ttcccagtcg tccggcatca ggatccgccg
     3301 caggtcgggg tggcccgtaa acgcgacccc gaacatgtcg tacacctcgc gctccggcca
     3361 cgccgccccg gggtagatgc cgacggcgga ggacagccgg gggggatccc cgtcgatctg
     3421 cactttgacg gtcagccggt cggggccggc cgcctgcccc aggaggtaga cgacttcaaa
     3481 ccgcggggac gcggggagac gatcccagca ggtgaggtcc accagggagt cgagcgcgag
     3541 gtgctggcgc gcggcctgga gcacctcgag cagcaccccg ggggtcgcca gcaagatcgc
     3601 ccggccccgc tcttccttcg gggtggtcag cccccacggc acatgggggc gcagccggtc
     3661 gaggatcggc gcctgcccca tcgatgccgt gtggctcagg gcgcccgccg ggcaggggat
     3721 ccctgggcga tcgcgtcttg gagaagccgg agcccgtcga ggagggcctc gggacgcggc
     3781 ggacagccgg ccacgtacac gtccacgggg aggatcttgt cgacgccctg cacgaccgcg
     3841 tagttgttgt agagcccgcc ggtgctggcg cagtccccca tggcgatcac ccacttcggc
     3901 tcgagcatct ggtcgtagat gtggcggacg accggggcca tcttctggct gacccgcccg
     3961 gcgacgatca tgaggtccga ctgccgcggg gacgcccgga acagctccgc gccgaaccgg
     4021 gcgatgtcga accgcggcgc ggcggtggac atcatctcga tcgcgcagca cgccaggccg
     4081 aactggaccg gccacagcga gctctttcgg gcccacgcca cgatgcggtc gagcgtggtg
     4141 gtcaggacgt tgcgctcgag gtcgcgttcg gaggcgatca ctcccattcg agcgccctcc
     4201 gccggaggag gtacagatac ccgcccccga ggacggcgag gaaggcgaac atctcgacca
     4261 gccccgcagg gcccagctcg cgcaccagca ccgcccacgg gtagatgaag acgatctcga
     4321 tgtcgaacag gatgaacagc atcgccacca ggtagtatcg gatggggacc cgctcccgcg
     4381 cgctgccgat cggccagacc ccggactcgt acggcagcga cttttccagg gttggccgcc
     4441 ggcctcccgc cagcctgtgg agcccgagca gggtgaccgc gagcagcacc gcgaggatca
     4501 ggtgcacgag cacaggagta tagcccgaca cacgtcaatg attgcagaga aggctacccc
     4561 tgtcaacgcg acggccacgc ggttgcgtcc gaagcccatg agcgcgaatg ccttgaggag
     4621 gcatcacgcc actcccagca atcgccgcgc gttgtcgcgc aggatcttcg gtctgaccgc
     4681 gtccttgatc ggcagcgctg caaagtcggc gagccaccgc tccggggtca tgaacggcca
     4741 gtccgacccg aacaaggcct tgtcctggag cacgctattg acgtgctgca cgagctcagg
     4801 agggaagtac ttcggcgacc accccgacag gtcgaggtag acgttcgact tatggcgggc
     4861 gatcgccagc tgctcggcct gccatggcca actgggatgc gccaggatga tctgaagcga
     4921 tgggaagtcg gccgccacat catcgagtaa cattggattg acatacttca gcttgtaccc
     4981 agccccaccc ggcaggcccg ctccggtggc catcgtaccg gagtgaaaga ggcacacaag
     5041 gccgagttcg gcggcggttg cccagagcgg atagaaccgc tcgtcgttgg cggcgaactt
     5101 ctgcaggccc ggatgcagct tcaggccgcg gagacccagc atctcatggg cgcggcgcgc
     5161 ctcgtcgacg gccaaccggc ctttccaggg atcaacgccg gcaaagccga taaagacgtc
     5221 tggatgggcc cgcacggccg aggcgatgtg gtcgtttggg acgggcggcc gccccgacgt
     5281 tgtggaactg gccttggcga gctgcacggc aatcattcgc tgcgcccggt actggtcggc
     5341 cagcttgtcg atggacaccg acggaatcga ccggccgaag tactccccca tggcctccag
     5401 gtcgctgccg aagcccccga tggcctccgg gctggtcgga tgaacgtgta cgtcgattgc
     5461 gaccaggtcc gccgtcatgc ccagtcttct tcccgcttga atccccaaag gccgctctgc
     5521 acctcccacc tttctcaatc cggtaggcag atctgcgctt cgacaccctg agcgaaggca
     5581 ccattcggcc aaggaatctc caacgccgtc caactccgcc ccgcgtccgg cgtaaggaag
     5641 accccatggt tgttcgccgc atacatgaca cccggttcac ctggcgtggc gaagcggctg
     5701 gcaatcgtgc cccggccctc cggcagcccc tccatcgccg tctgaaagga accccgcgcc
     5761 gtcttccgat agacatacgc ctccgcccgg cgcgggctgt aggccacaaa gggtccggac
     5821 g
//
LOCUS       DASQGH010000499         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53057, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000499 DASQGH010000000
VERSION     DASQGH010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53057"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..966)
                     /gene="fdhD"
                     /locus_tag="VEW91_09835"
     CDS             complement(61..966)
                     /gene="fdhD"
                     /locus_tag="VEW91_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HYM91919.1"
                     /translation="MGDASRDPATASVRTSTTRRRVGQVQEGVVRVRDDALVVEEPLE
                     IRLYPGDGEPYLQVSVTMRTPGHDFELAAGFLFTEGILQNCGQVDHINYCADRTLEQA
                     QRYNIVNVYLRPGVPLDPERLRRNFYTTSSCGVCGKASIEAIQVRGLRPVADDGFAVD
                     GEVFARMGDVLRRSQAIFDKTGGLHAAALFDPSGGLLAVREDVGRHNAVDKLVGHAFL
                     DRALPLSRHLLMVSGRASFEIMQKAAAAGVPVVAAVSAPSSLACDVARAFGMTLVGFA
                     RGDRFCVYAGAHRIRQRGQPLPGID"
     gene            complement(959..>2488)
                     /locus_tag="VEW91_09840"
     CDS             complement(959..>2488)
                     /locus_tag="VEW91_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HYM91920.1"
                     /translation="VRPGEVPGGGPERGEAWAIATGAPMPARGDRVLPVEAVRHAGDH
                     VRVELPPGEKTHVAAPGEAIREGARLLVAGEAIRPHAAGALSACGVGAVRVHRRPRVG
                     LVATGDELVDATGEGVGLPPGRVFNSNAVAVAGLLRVAGCEVEYRGLVRDAPEQMREA
                     FQALLGRYDVVLSTGGVSVGRHDAVHRTWLDLGVERIVGRVDLKPGGPFFAGRARDTW
                     VVGLSGTPVACLAAFHLLARPFLLRLAGRRWVVRPVLPVTLATGFPKATARMRALWAR
                     VEAGAGGRPTAEVLPRAAAGDVASLLGTNALVLIPPGTPPLPRGSRATALLLDHEEDR
                     DHLTIDPPSPGPMVVGVIGESGGGKTTILADLVRRLAGDGIRAVAVKHAAHGFDLDRR
                     GSDSARMADSGAGIVVLAGPTETVIRVAAPVDDPERAVALATAIAEEMWGAPPALVLI
                     EGFPHPAGPVIQVGPQKADVSAGEVLARVPAVARLDEEALATELRKVVEAVRARVWPH
                     G"
BASE COUNT          350 a          971 c          857 g          310 t
ORIGIN      
        1 ggatcaggtt gttggcttcg ggccagtgga ccgcgaggtt gccgggcgcg cgccgccccc
       61 tcagtcgatc ccgggaaggg gctgtccccg ctgccggatc cggtgggcgc cggcatagac
      121 gcaaaaccga tctccccgcg cgaaccctac cagggtcatc ccgaaggccc gggccacgtc
      181 gcacgccagg ctcgacggcg ccgagacggc cgcgaccacc gggacgccgg ccgcggcagc
      241 cttctgcatg atctcgaagc tggcccggcc gctgaccatc aggaggtgcc tgctcagggg
      301 gagcgcccgg tcgaggaagg catgccccac gagcttgtcc acggcgttgt ggcgcccgac
      361 gtcctcgcgc accgcgagca gcccgccgct cggatcaaac agcgcagcgg cgtgaagccc
      421 gccggtcttg tcaaagatcg cctgcgaccg gcgcaggacg tcccccatcc gggcgaagac
      481 ctccccgtcc acggcgaacc cgtcgtcggc gaccgggcgg agaccccgca cctggatcgc
      541 ctcgatcgag gccttcccgc acaccccgca gctggaggtc gtgtagaagt tgcgccggag
      601 gcgttcgggg tccagcggga cgcccggcct caggtagacg ttcacgatat tgtatcgctg
      661 ggcctgctcg agggtgcggt ctgcgcagta gttgatgtgg tccacctgcc cgcagttctg
      721 taagatcccc tcggtgaaga ggaagcctgc ggcgagctca aagtcatgcc ccggcgtccg
      781 catggttacg gagacctgca ggtagggctc tccgtccccg gggtagagcc ggatctcgag
      841 cggctcctcc accacgagcg catcgtcgcg gacccgcacg accccttcct gcacctgtcc
      901 gacacgccgc cgtgtcgtgc tggtgcggac ggatgctgtg gcgggatcgc gagacgcatc
      961 acccatgggg ccacacgcgc gcgcggaccg cttcgaccac cttccggagc tcggtcgcga
     1021 gcgcctcctc atccaggcgg gcgaccgcgg gcaccctcgc cagcacctca ccggccgaga
     1081 cgtcggcttt ctgcgggccg acctggatca ccggcccggc tgggtgcggg aagccctcga
     1141 tgagcacgag ggcgggaggg gccccccaca tctcttccgc gatcgcggtg gcgagcgcca
     1201 ccgcccgttc aggatcgtcc accggggccg ccacgcggat cacggtctcg gtgggcccgg
     1261 cgagcacgac gatccccgcc cccgagtcgg ccatccgggc gctgtcgctc ccccggcggt
     1321 cgaggtcgaa cccatgggcg gcgtgcttca ccgccacggc gcggatgccg tcgcccgcca
     1381 gccgccgaac gaggtcggcg aggatggtgg tcttcccgcc ccccgactcc ccgatgaccc
     1441 cgacgaccat cggccccggg ctcggcggat cgatcgtcag gtggtcccgg tcttcctcgt
     1501 ggtcgagcag cagcgccgtc gcgcgcgacc cccgcggcaa cggcggggtc ccgggcggga
     1561 tgagcaccag ggcgttggtg ccgaggagcg aggcgacatc gcccgcggca gctcgcggca
     1621 gcacctcggc cgttgggcgc ccccctgctc cggcctccac ccgcgcccac agcgcgcgca
     1681 tccgggcggt cgccttgggg aacccggtgg cgagcgtgac gggcagcacc ggcctgacca
     1741 cccagcgcct gccggccaac cgcagcagga atggccgggc gagcaggtgg aacgcggcca
     1801 ggcaggcgac cggggtgccg gagagtccca cgacccacgt gtcgcgcgcc cggccggcga
     1861 aaaacggccc cccgggcttg aggtcgaccc gcccaacgat ccgctccacc ccgaggtcga
     1921 gccaggtgcg gtggaccgca tcgtgccggc ccaccgacac gcccccggtg gagagcacca
     1981 catcgtaccg cccgaggagt gcctggaacg cctcccgcat ctgctccgga gcgtcacgga
     2041 cgagcccgcg gtactccacc tcgcatcccg ccacccgaag gaggccggcg acggcgaccg
     2101 cgttgctgtt gaacacccgg ccgggaggga gacccacgcc ctccccggtt gcgtccacga
     2161 gttcatcccc ggtcgcgacc agccccaccc gcggccggcg gtggacgcga accgccccga
     2221 cgccgcatgc ggacaacgcg ccggcggcgt gcggccggat cgcttcgccg gcgacgagca
     2281 gccgagcgcc ctcccggatg gcttcccccg gggccgccac gtgggtcttc tccccgggcg
     2341 gcagttcaac ccgcacatga tccccggcgt ggcggaccgc ttccaccggc agcacccggt
     2401 ccccgcgcgc aggcatcggg gcgccggtcg cgatcgccca cgcttccccc cgctctggcc
     2461 cgccgcccgg gacctccccg ggccggac
//
LOCUS       DASQGH010000500         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55776, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000500 DASQGH010000000
VERSION     DASQGH010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55776"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1464)
                     /locus_tag="VEW91_09845"
     CDS             complement(100..1464)
                     /locus_tag="VEW91_09845"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HYM91921.1"
                     /translation="MAPRGASAVRINLAHTLELLHTHITAALCQTVFATVRTTERQRA
                     WTLDALVQFWLAVILRAPKALSHALAEALDGTVPLVHPVPATPEAFFQRCRDLRPAFF
                     AAVFAEFTRRLLPRARPTYAGELAALQARFAAVLVVDGSTLAAIAHRLKLLWPERTVI
                     LPGCLLGLYDLGRGLCRGLGFAPDAAAAELPRAVAALPQCPRDALLVGDRLYCAAAWF
                     RALTQHGCWGLVRRNRPLGLRRLRRLRQRRYRGGRLEEWLVAAGARDSVVLRHIRWHR
                     GRTAHELLTNVLDPQRLPAVEALALYPARWTIERMYYDLKEVLNLNRVYAANPNAIAM
                     QLYAAACVYNAVRVAQAQVAAAARLAPEALSPAKLFPRVAAACQAHAVLEWSFGELQR
                     ANPGRRLRRPVLHRQRFASVALATIRVEPRTGPRRRPGFYEGRPPWKSLAHVRGARKV
                     LKLT"
BASE COUNT          245 a          528 c          623 g          189 t
ORIGIN      
        1 gaccgccaag cgcgcggcag ccttcttcgc gcagatgccc aagccgcagg catgagccgg
       61 actgcacggc gtaggggcgg accttcaggt ccatcgccgc taagttaatt tgagcacctt
      121 ccgggcccca cgcacatgcg cgagcgattt ccaagggggc cggccctcat agaacccggg
      181 ccgccgccgc gggcccgtgc gaggctccac ccggatcgtg gccagggcca cggaggcgaa
      241 gcgttgccgg tgcagcaccg gacggcggag gcgccggccg gggttcgccc gctggagttc
      301 gccaaaggac cattcgagca ccgcgtgcgc ctgacaggcc gccgccaccc gcgggaagag
      361 cttggcgggc gagagggcct cgggcgccag ccgggccgcg gcggcgacct gggcctgagc
      421 cacccggacg gcgttgtaca cacaggcggc ggcgtagagt tgcatcgcga tggcgttcgg
      481 gttcgccgcg tagacgcggt tgaggttcag gacctccttc aggtcgtagt acatgcgctc
      541 gatggtccag cgcgcggggt agagggccag ggcctccacg gcgggcagcc gttgcgggtc
      601 gaggacgttg gtcaggagct cgtgcgccgt ccggccccgg tgccagcgga tgtgccgcag
      661 gaccaccgag tcgcgggccc cggccgccac cagccactcc tccaggcgcc cgccccggta
      721 gcggcgctgg cggaggcggc ggagccgccg gagccccagg ggccggttgc ggcggacgag
      781 gccccagcag ccgtgctggg tcagggcccg gaaccaggcc gccgcacagt agagccggtc
      841 gcccacgagc agggcgtccc ggggacactg gggcagggcg gccacggccc ggggcaattc
      901 ggcggccgcc gcgtcgggag cgaagccgag cccccggcag aggccccggc cgaggtcgta
      961 gaggcccagg aggcagcccg gcaggatgac cgtgcgctcg ggccagagga gcttgagccg
     1021 gtgggcgatg gccgccaggg tggacccgtc caccaccagc accgcggcga accgggcctg
     1081 gagggccgcc agctcccccg catacgtggg gcgcgcgcgg gggaggagcc gccgggtgaa
     1141 ctcggcgaac acggccgcga agaaggccgg gcggagatcc cggcagcgct ggaagaaggc
     1201 ctcgggggtg gcggggaccg ggtggacgag cggcaccgtc ccatcgagcg cttcggccag
     1261 cgcgtggctc agggccttcg gcgcccggag gatcacggcg agccagaact ggacgagggc
     1321 gtccagggtc caggcgcgct ggcgttccgt ggtccggacg gtggcaaaga cggtctggca
     1381 gagagcagcg gtgatgtgag tgtgcagcag ctccagggtg tgggcgaggt tgatccgcac
     1441 cgccgatgcg ccgcgtgggg ccatagcacc tccttggctg gcaggctcta tggcagccca
     1501 gtataaacgc aacttgggct ctctgtgcta ggctgttctg gaccatagcg aaattatctt
     1561 agcggcgatg ggcataaagc ccgcc
//
LOCUS       DASQGH010000501         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50314, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000501 DASQGH010000000
VERSION     DASQGH010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50314"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VEW91_09850"
     CDS             complement(<1..491)
                     /locus_tag="VEW91_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYM91922.1"
                     /translation="MIFNGLLSRFTRDMGVDLGTANTLVYVRHEGIVLREPSVVAKRV
                     DGGEILAVGDEAKKMIGRTPGDIVATRPLRDGVIADFDTTAAMLTYFIRNGLRGRSFL
                     RPRVVVGIPSGVTEVEKRAVIDATLQAGAREAYLIEQPMAAAIGAGLPVSEPVGSLVV
                     DIGG"
     gene            complement(488..1195)
                     /gene="radC"
                     /locus_tag="VEW91_09855"
     CDS             complement(488..1195)
                     /gene="radC"
                     /locus_tag="VEW91_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYM91923.1"
                     /translation="MKDLPLELRPRERMLAEGPAALSAAELLAVLLRTGSRTRSALAV
                     ATDLLCAHGSLERLAGASPSELRRTGGVGEVKALHLLAAFELGRRLGALPARARPAVR
                     RPADAAALVMPDLRFQETERFWLLLLNTKHEVLDRVEVSRGGLASSPVHPREVFRAAL
                     RHSAAGVILVHNHPSGDPTPSRADLAMTARLSRAGGVMGIPIIDHIIIGDGRYVSLRE
                     RGASFDPVPPQERSVDG"
     gene            complement(1389..1658)
                     /locus_tag="VEW91_09860"
     CDS             complement(1389..1658)
                     /locus_tag="VEW91_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HYM91924.1"
                     /translation="MVKPSKRGRNPWWVLVVIVIAGAMLGSVLANAIGQFTYLSWLGR
                     SVAVGLAPPVVIDLQVMTLTVGFTLRLNLAILLGILVAAYVFRML"
     gene            complement(1787..1969)
                     /locus_tag="VEW91_09865"
     CDS             complement(1787..1969)
                     /locus_tag="VEW91_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYM91925.1"
                     /translation="MGPKFWELIIVLAIVILLFGPSRLAGLGGAVGKAMREFREATKP
                     EEPKSPAPEKGTGEKS"
     gene            complement(2070..3059)
                     /locus_tag="VEW91_09870"
     CDS             complement(2070..3059)
                     /locus_tag="VEW91_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYM91926.1"
                     /translation="MPTLVDLARIYEPVREDLDAVARLLREELSADDPFIGELVAHVL
                     QAKGKLLRPALVCLSALACGGGSAARLPVGGACELIHVASLIHDDVIDAADLRRGQAT
                     VNAVWGNQVAVLLGDYLFSKAFDLLARLHDADLAAAMAGATVKMSQAEIKQIRYGNAP
                     HTNPTIYFDIIEGKTAHLFSAACRCGALTARAPEPAARALSEFGLEWGMAFQITDDAL
                     DLTATPQQVGKPIGSDIQTGKVTLPVIHALAQAPEPDRRRLEELLNSGGDRHPEAAAE
                     VGRILARCGSIEHALRVARAHTARAAASLARLPGSPARDSLVALVEFVTERTR"
BASE COUNT          514 a         1163 c         1060 g          449 t
ORIGIN      
        1 ccgccgatgt ccaccaccag gctgccgacc ggttctgaga ccggcagccc ggccccgatc
       61 gccgcggcca tcggctgctc gatcaggtac gcttcgcggg cgccggcctg gagcgtggcg
      121 tcgatgacgg cgcgcttctc gacctcggtc actccgctgg ggatccccac caccacccgc
      181 ggccggagga agctgcggcc gcgcaggccg ttgcggatga agtaggtgag catcgccgcc
      241 gtcgtgtcga agtcggcgat cacgccgtcc cgcagggggc gggtcgcgac gatatcaccc
      301 ggggtccggc cgatcatctt cttggcctcg tctccgactg cgaggatctc gccgccatcc
      361 acccgcttgg ccaccacgga gggctcgcga agcacgatgc cctcgtgccg cacgtacacc
      421 agcgtgttgg cggtcccgag atccacgccc atgtcccgag tgaaccggct cagcagtccg
      481 ttgaagatca tccgtctaca ctcctctcct gcggtgggac cgggtcgaag ctcgcgcccc
      541 gctcccggag actgacgtag cgtccgtctc caatgatgat gtggtcaatg ataggaatac
      601 ccattacacc cccggcccgg ctcaggcggg cggtcatcgc caggtccgcc cggctgggcg
      661 tggggtcccc cgacgggtgg ttgtgcacca ggatcacccc cgcggcgctg tgccgcagcg
      721 ccgccctgaa cacctcccgc gggtggaccg gggagctggc cagccctccc cgggacacct
      781 cgacccggtc caggacctca tgcttggtgt tcaggagcag gagccagaag cgctcggtct
      841 cctgaaagcg caggtcgggc atgacgagtg cggcggcatc ggccggccgg cgcacggcgg
      901 gccgggcccg ggcgggcagg gcgccaagcc gccggcccag ttcgaacgct gccaggagat
      961 ggagcgcctt tacctcccct acccccccgg tccggcgcaa ctcgctgggg ctcgcccctg
     1021 ccaaccgctc caggctgccg tgggcgcaca ataggtcggt ggccaccgcg agggcgctcc
     1081 gcgtccgcga ccccgttcga agcagcacgg ccagcagttc ggcggcgctg agggcggccg
     1141 gcccctcggc aagcattcgc tcgcgggggc gcaactccag cggaagatcc ttgatccgag
     1201 gcgacgagac cggggcggca gccctcggat cccgtctcgg cgacgggtta agccggggag
     1261 agacacgggc gagcactcgg cggctcctcc aaccgggggg cggcctcctc agaggccgcc
     1321 acatgcatgc agggggcgag gccggcgacg cctctcgaca cgccatcctc gcgaccgttg
     1381 agtagcgatc agagcatccg aaacacgtat gcagcgacca gtataccaag gagaatagcg
     1441 aggttcaacc gaagcgtgaa gcccaccgtc agcgtcatga cctgcaggtc gatgacgacc
     1501 gggggcgcca agccaaccgc aaccgaccgg cccagccagg acaggtaggt gaactggccg
     1561 atagcgttcg cgagcacgct ccccaacatc gcccccgcaa tgacgatcac gacgagcacc
     1621 caccacgggt ttcggccccg tttggaaggt ttgaccacgc atcaccccct tccgccccgt
     1681 cccacgccgg gggtctcacc ggcccgacga cgcacagaaa aaggagcggc tcctggccgc
     1741 tcccctcgtc gatctccggc cgcttcccgc tccggggccg gagcgatcag ctcttctctc
     1801 cggtgccctt ctcgggcgcc gggctcttcg gctcctcagg cttcgtggcc tcgcgaaact
     1861 cgcgcatcgc cttgcccacc gcgccgccca gacccgccag ccggcttggc ccaaacagta
     1921 ggatcacaat tgcaagcacg atgatgagtt cccaaaactt cggccccata ccgcacctcc
     1981 gacttcacta tacactatac accccagcga agcgcgacgc caccgggttc ccccctagcg
     2041 gcgataggcc cctaacgggg ccaggccccc tatcgggttc gctcggtgac aaactccacg
     2101 agcgcgacca ggctgtcccg ggccggggag ccgggaagcc gggcgaggct cgcggcggcg
     2161 cgggcggtgt gggcccgggc cacccgaagg gcgtgttcga ttgacccgca gcgggcgagt
     2221 atccgtccca cctccgccgc ggcttcgggg tgccggtcgc ccccgctgtt gaggagctcc
     2281 tccaggcgcc ggcggtcggg ctcgggggcc tgggccaggg cgtggatcac cggcagggtg
     2341 accttgcccg tttgaatgtc gctcccgatc ggcttgccga cctgctgcgg ggtcgccgtg
     2401 agatcgaggg cgtcatccgt gatctgaaac gccatccccc attcaaggcc gaactcggag
     2461 agggcccggg cagccggctc cggggcccgc gcggtgagcg ccccacaccg gcaggcggcg
     2521 ctgaagaggt gcgcggtctt tccctcgatg atgtcgaagt agatggtcgg gttggtgtgc
     2581 ggcgcattgc cataccggat ctgtttgatc tccgcctggc tcatcttgac cgtcgccccg
     2641 gccatcgcgg cggccagatc cgcgtcgtgc aggcgggcca gcaggtcaaa cgccttgctg
     2701 aacaggtagt cccccagcag caccgccacc tggttgcccc agaccgcgtt cacggtggcc
     2761 tgcccgcggc ggagatcggc cgcgtcgatc acgtcgtcgt ggatcaagga cgcgacgtgg
     2821 atgagttcgc aggccccgcc gaccggaagg cgagcggcgc tccctccccc gcaggccaac
     2881 gcgctgagac agaccagggc cggccgaagg agcttcccct tcgcctgcag gacgtgcgcc
     2941 accagctcgc cgatgaacgg gtcgtccgcg gagagttcct cgcggagcag gcgcgcgacg
     3001 gcgtccagat cctcccggac gggttcgtag atccgggcga ggtccacgag cgtcggcacg
     3061 atactacgct tccgccacca cgccgaagag ggcgacaaag aacagcagac cgaagaacaa
     3121 gagccagaag atcgcgtacc gccagaacac ggtcgcgccg gccgggcccg gcgccggggc
     3181 cgccgg
//
LOCUS       DASQGH010000502         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53068, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000502 DASQGH010000000
VERSION     DASQGH010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53068"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VEW91_09875"
     CDS             <1..852
                     /locus_tag="VEW91_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HYM91927.1"
                     /translation="VNGQEYRGVVEIRRTPAGRLTAINELDLEQYLYGVIKGEIDPRW
                     PPEAVKAQAVAARTLALETLAASRGKYGGEGYTLRATTDSQVYLGVAGEDPGAAAAVD
                     ATRGIVATFQGQPIFAAYHSNSGGHTEDSENVWGTRHPYLRGVPDPYVLDAPGGVWTA
                     RLPLATIEVDLRRGGVNVAGIAAIAPGRVTPWGRVITVRLLDEGGRAQEIGANQFRLL
                     VGPTVVRSTMFVITREGAEVGFSGRGSGHGVGLDQWGARAMALRGYTFEQILAYYYTG
                     ISIERRF"
     gene            869..>1709
                     /gene="queA"
                     /locus_tag="VEW91_09880"
     CDS             869..>1709
                     /gene="queA"
                     /locus_tag="VEW91_09880"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HYM91928.1"
                     /translation="MRLSDFDFALPPELIAQSPARPRDGARLMVLHRATGELVHRVFR
                     DLPAYLRPRDVLVLNDTKVLPARLRGRRSTGGVVEMLLLRPVSGDAWEALLRPGRRIP
                     EGARLVFAPGVLEATVGARTTAGTRVVELEHHEDLHSLLLRIGEMPTPPYITRRLEDP
                     SDYQTVFARAEGAVAAPTAGLHFTDGLLGAIRGQGIQVALLTLHVGLGTFRPVRTEEI
                     ARHRMDAEAYEIGREAAAAINGARPAGGRIVAAGTTCVRALESAADADGRVPAGSGST
                     ALFI"
BASE COUNT          234 a          573 c          655 g          247 t
ORIGIN      
        1 gtgaacgggc aggagtaccg cggggtggtc gagattcgcc ggaccccggc cgggcggctc
       61 accgcgatca acgaactcga cctggaacag tacctctacg gggtcatcaa gggagaaatc
      121 gacccccgct ggccgcccga ggccgtgaag gcgcaggcgg tcgcagcccg gacgctggcg
      181 ctcgagaccc tggccgcatc gcgcgggaag tatggggggg agggctacac gctgcgtgcc
      241 accaccgact cgcaggtgta cctgggcgtg gccggcgagg accccggcgc ggccgcggcg
      301 gtggacgcga cccggggcat cgtggcgacg ttccaggggc agccgatctt cgccgcctac
      361 cattcgaact cgggcgggca caccgaggac agcgagaacg tgtgggggac tcggcatccg
      421 tacctgcggg gggtgccgga tccctacgtg ctcgatgcgc ccgggggggt gtggacggcg
      481 cggttgccgc ttgcgacgat cgaggtcgat ctccgccgcg gcggggtgaa cgtcgccggg
      541 atcgcggcga tcgcgcccgg ccgcgtgacg ccctggggcc gcgtgatcac cgtgcgcctt
      601 ctcgacgagg gggggcgggc gcaggagatc ggtgccaatc agttccgcct cctggtcgga
      661 cccaccgtcg ttcggagcac gatgttcgtc atcacgcgcg agggcgccga ggtcgggttc
      721 agcgggcggg gctccgggca cggcgtcggc ctggatcagt ggggcgcgcg ggccatggcc
      781 ctccgggggt atacgttcga gcagatcctc gcgtactatt acaccgggat ctcgatcgag
      841 cggcggttct agccggaccc gcgctcgcat gcggctctcg gactttgatt ttgctctgcc
      901 cccggaactg atcgcgcagt cgcccgcgcg gccccgggat ggtgcgcggc tcatggtact
      961 gcaccgggcg accggggaac tcgtccaccg cgtgtttcga gacctgcccg cctatcttcg
     1021 acctcgggat gtgctggtgc tgaacgacac gaaggtcctg cccgcccgcc tccggggacg
     1081 ccggtcgacc gggggggtgg tggagatgtt gctcctgcgg ccggtgtcgg gcgatgcctg
     1141 ggaagcgctc ctgcggcccg gtcgcagaat ccccgagggc gcgcgcctcg tgtttgcccc
     1201 cggcgtgctc gaagccacgg tgggcgcgcg caccaccgcc ggaacccggg tggtcgaact
     1261 cgagcaccac gaggatctgc attcgctcct tttgcggatc ggggagatgc cgacaccgcc
     1321 gtacatcacg cggcggctcg aggatcccag cgactaccag acggtgttcg cgcgggccga
     1381 gggagcggtc gcggccccga cggccgggct ccacttcacc gacgggttgc tcggggcgat
     1441 ccgggggcag gggatccagg tcgccttgct cacgctccac gtggggctcg ggactttccg
     1501 cccggtcagg acggaggaga tcgcccgcca ccgcatggac gcggaggcct acgagatcgg
     1561 acgcgaggcg gcggcggcca tcaacggcgc gcgccccgcg gggggccgga tcgtcgctgc
     1621 ggggaccacc tgcgtccgcg ccctggaatc ggcggcggac gcggatgggc gcgtaccggc
     1681 gggaagcggc agcaccgcgc tgttcatca
//
LOCUS       DASQGH010000503         1757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33405, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000503 DASQGH010000000
VERSION     DASQGH010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33405"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VEW91_09885"
     CDS             <1..420
                     /locus_tag="VEW91_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYM91929.1"
                     /translation="RWDPFEDVGTLRRSMERLFDDVFARDPANAAWEPAVEMFETDHE
                     VVVRAELPNIDPKQVDITVTNDTMTLKGESKHEQEEQGRNYYRRELRYGTFTRTLPLG
                     TEVKSGDAKAVYKDGVLEIKIPKSERVKPTSVKVQAG"
     gene            512..>1757
                     /gene="dnaK"
                     /locus_tag="VEW91_09890"
     CDS             512..>1757
                     /gene="dnaK"
                     /locus_tag="VEW91_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYM91930.1"
                     /translation="MAKVVGIDLGTTNSVIAVMIGGEPTVIPNAEGSRLTPSVVGFTK
                     TGERLVGQMARRQAILNPENTVYSIKRFMGRRLNEVEAERRIVSYKVEEGKGGTAVVN
                     IPAAGKTFTPEEISAMILQKLKADAEAYLGEKVEQAVITVPAYFNDAQRTATRNAGEI
                     AGLKVLRIINEPTASALAYGRQLEQKHAKTILVFDLGGGTFDVSILEIGEGVYEVKAT
                     NGDTHLGGDDFDDRIVNWLADEFRKQQGIDLRQDRQALQRLREAAERAKIELSTVVQT
                     SINLPFVTADASGPKHLDMVLTRARFDELTADLVERCIGPFRQALADAKLSERDLNEV
                     ILVGGATRMPSVQELVRRLTGKEPNKEVHPDEVVAVGAAIQAGVLAGEVREVVLLDVT
                     PLSLGIETLGGVDTVLISRNTTI"
BASE COUNT          342 a          549 c          623 g          243 t
ORIGIN      
        1 cggtgggacc cgtttgagga cgtgggaacc ctgcggcgat cgatggagcg gttgttcgac
       61 gatgtcttcg cccgcgaccc cgcgaatgcg gcctgggagc ctgcggtgga gatgttcgaa
      121 accgaccacg aggtcgtcgt ccgggcagaa ctgcccaaca tcgatcccaa gcaggtggac
      181 attaccgtga cgaacgacac catgacgctc aagggtgagt cgaagcacga gcaagaagag
      241 cagggccgca actactaccg gcgggagctt cggtacggca ccttcacgcg gaccctcccg
      301 ctcggaaccg aggtcaagag cggggacgcg aaggcggtgt acaaggacgg cgttctggag
      361 atcaagattc cgaagtcgga gcgggtcaag ccgacctcgg tgaaggtgca ggccggttaa
      421 ggatccgggc aacgcgcgcg ccgtcgcccg gcgcacccgg gcgacggcca acaccgggac
      481 gcggcgggac cggccgccgg gaggaatcgg tatggcgaag gttgtgggta tcgatcttgg
      541 caccaccaac tccgtgatcg cggtgatgat cggcggcgag ccgaccgtca tccccaacgc
      601 cgagggcagc cgcctgacgc cctccgtcgt gggtttcacg aagaccgggg agcgcctggt
      661 cggccagatg gcccggcggc aggcgatcct caaccccgag aacaccgtct attcgatcaa
      721 gcggttcatg gggcggcgcc tcaacgaggt ggaggccgag cggcggatcg tctcctacaa
      781 ggtcgaggaa gggaagggcg gcaccgcggt cgtcaacatc ccggcggccg gcaagacgtt
      841 cacgcccgaa gagatttcgg cgatgatcct ccagaagctc aaggccgacg ccgaagccta
      901 cctaggcgag aaggtcgagc aggcggtgat cacggtcccg gcctacttca acgacgccca
      961 gcgcacggcg acgcggaacg cgggcgagat cgccgggctc aaggtgctgc gcatcatcaa
     1021 cgagccgacc gcgtccgcgc tggcctacgg aaggcagctg gaacagaagc acgccaaaac
     1081 gatcctcgtc ttcgatctgg gcgggggaac gttcgacgtc tcgatcctgg agatcgggga
     1141 gggcgtctac gaggtcaagg ccaccaacgg cgacacgcat ctcgggggag acgacttcga
     1201 cgaccgcatc gtcaactggc tggcggacga gttccgcaag cagcagggga tcgacctgcg
     1261 gcaggaccgc caggcgctcc agcggctgcg cgaggctgcg gagcgggcga agatcgagct
     1321 cagcaccgtc gtgcagacat cgatcaacct gccgtttgtc accgcggatg cgtcagggcc
     1381 caagcacctg gatatggtgc tcacccgtgc gaggttcgac gagttgaccg cggacctggt
     1441 ggagcggtgc atcggcccgt tccggcaggc cctcgccgac gcgaagctga gcgagcgaga
     1501 cctcaatgaa gtcatcctgg tcggcggcgc cacccggatg ccgagcgtcc aggagctcgt
     1561 gcggcgcctg acgggcaagg aacccaacaa ggaggtccac ccagatgagg tggtcgccgt
     1621 gggcgcggcg atccaggccg gggtgctggc gggcgaggtc cgggaggtcg tgctcctgga
     1681 cgtcaccccg ctctcgctcg gcatcgagac cctcggtggg gtggacaccg tcctgatctc
     1741 gcgcaatacg acgattc
//
LOCUS       DASQGH010000504         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27622, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000504 DASQGH010000000
VERSION     DASQGH010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27622"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1897
                     /locus_tag="VEW91_09895"
     CDS             56..1897
                     /locus_tag="VEW91_09895"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HYM91931.1"
                     /translation="MAGGPLLCDGAMGTLLYERGVPFDRCFDALNLTDRERVLNVHLD
                     YLRAGAEMIETNTFGANAVKLGAHGLGDRVRDVNWHGAKIAKEARDIIGQAVWIAGSV
                     GPLGKPVAPFGRISAAEAGAAFRAQIDALVEGGVDVLILETFTDLEELLEAVRAARTA
                     CDLPVIAQMSFTEDGRTRYGHDPAEVVAGCEQAGVDVIGANCSVGPVPMLDVIQRLAA
                     LTRRPLSAQPNAGFPTLVDGRYLYLSSPTHMAMYARKMVEAGAVIVGGCCGTTPAHIS
                     AIGQAIMDLRPAAVRAVGPEGPARAEEPVAPPVDRPTQLAGKLGRGFVISVEVDPPKG
                     LDATKDLEGARLLKEAGADVIDVGDSPIGRVRMGALAMCFVIQQQVGIETIIHFTTRD
                     RNLMGVQADLIGAHALGVRNILALTGEPPRGDYPNVTAVFDVDSVGLVRIIRRFNEGL
                     DLSGKSIGRPGRFLIGCGLDMNPETLDRELPRLERKLEAGVDFFMTQPVYEPETLDAF
                     ERRAGKLRVPVLVGILPLQSLRHAEFLHNEVPGIRIPRWVRDRMQAAGSAGRDEGLRL
                     ARELLEALLGRIDGAYFMPSFGRYELVATLVREVRARVMANAARPTG"
     gene            1894..>2257
                     /locus_tag="VEW91_09900"
     CDS             1894..>2257
                     /locus_tag="VEW91_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91932.1"
                     /translation="MNPASLYLDYVRSAAVVTLAFYTAGHAAIGLPLLLIGDAWRLVT
                     RRRVLWHPTAVDLPLATFGVILLLSTAASPYHRIAVDATFVTVLSGVVFFGAFTWLLH
                     QAPNVRVTLLRAWALGAPP"
BASE COUNT          330 a          748 c          809 g          370 t
ORIGIN      
        1 gtccggaact tcgcctgacc ccataacctc gacgcggctt ccctttctgg cccgcctggc
       61 gggcgggccg ctcctgtgcg atggagccat gggtaccctg ttgtacgagc ggggggtgcc
      121 gttcgaccgg tgcttcgacg ccctcaatct gaccgaccgg gaacgcgtgc tgaacgtcca
      181 cctcgactac ctccgcgccg gggccgagat gatcgagacc aacacctttg gcgccaacgc
      241 ggtgaagctg ggcgcccacg gcctgggcga ccgtgtccgg gacgtcaact ggcacggcgc
      301 gaagatcgcg aaagaggcgc gggatatcat cggccaggcg gtatggatcg cagggtccgt
      361 gggcccgctc gggaagcccg tagcgccgtt tggccggatc tccgcggccg aggcgggcgc
      421 cgccttccgg gcccagatcg acgccctggt cgaggggggc gtggacgtgc tcatcctgga
      481 gacgttcacc gacctcgagg agctgctgga agcggtccgg gccgcgcgga ccgcgtgcga
      541 cctccccgtg atcgcccaga tgagcttcac cgaggacggc cggacgcgct acggacatga
      601 cccggcggag gtcgtcgccg gatgcgagca ggcgggggtc gatgtcatcg gagccaactg
      661 cagcgtgggc ccggtgccga tgctggacgt gatccagcgg ctggccgcgc tgacccgcag
      721 gccgctctct gcccaaccaa acgccgggtt tcccaccctg gtcgatggcc ggtacctgta
      781 cctgtcctca ccgacgcaca tggcgatgta tgcccggaag atggtcgaag ccggagcggt
      841 catcgtcggc ggctgctgcg gcaccacccc ggcgcatatc tcggcgatcg ggcaggcgat
      901 catggatctc aggccggccg cggtccgcgc ggtgggcccc gagggcccgg cccgcgcgga
      961 ggagccggtc gcccccccgg tcgaccggcc cacccagctg gccgggaagc tggggcgggg
     1021 attcgtcatc agcgtggagg tcgacccgcc gaagggcctg gacgcgacga aagacctgga
     1081 gggcgcccgc ctgctcaagg aggccggagc ggacgtgatc gatgtggggg acagcccgat
     1141 cggacgggtc cggatggggg ccctcgcgat gtgcttcgtg atccagcagc aggtggggat
     1201 cgagacgatc atccatttca cgacccggga tcgcaacctc atgggcgtgc aggccgacct
     1261 gatcggcgcc cacgccctgg gggtgcgcaa tatcctggcg ctgaccggcg agccgccccg
     1321 gggggactac ccgaacgtca ccgccgtgtt cgacgtggac agcgtggggt tggtgcggat
     1381 catcaggcga ttcaacgagg gattggacct ctcgggaaag tcgatcggcc ggccggggcg
     1441 gttcctcatc gggtgcgggc tcgatatgaa ccccgagacg ctcgaccgcg agctgccgcg
     1501 gctcgagcgc aagctggagg ccggagtgga tttcttcatg acccagccgg tctacgaacc
     1561 agagacgctc gacgccttcg agcgccgggc gggcaagctg cgggtgccgg tgttggtcgg
     1621 gatccttccg ctccagagcc tccgccacgc cgagttcctg cacaacgagg tgccggggat
     1681 caggattccg cggtgggtcc gggaccgcat gcaggccgcc gggagcgcgg ggcgagacga
     1741 gggcctccgc ctggcgcgcg agttgctgga ggcattgctc ggccgaatcg atggcgccta
     1801 cttcatgccc tccttcggcc ggtacgagct cgtggccacc ctcgtccggg aagtcagggc
     1861 gcgggtcatg gcgaatgcgg cgaggcccac cggttgaatc cggcgagcct ctacctggac
     1921 tatgtccgat ctgcggcggt cgttactctg gcattttaca ccgccgggca tgcggcaatc
     1981 ggacttcccc tcctcctcat tggcgacgcg tggcgcttgg tgacgcgtcg cagggtgtta
     2041 tggcatccca cagcggtgga cctgcctctg gccactttcg gcgtgatcct gttgctgtcc
     2101 accgccgcgt cgccgtacca tcggatcgca gtcgatgcca cgttcgtgac tgtcctctcc
     2161 ggggtggtgt tctttggagc gttcacgtgg ctcctgcacc aggcgcccaa tgtccgagtc
     2221 actctgctga gggcctgggc gttgggagct cctcccg
//
LOCUS       DASQGH010000505         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33414, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000505 DASQGH010000000
VERSION     DASQGH010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33414"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1487)
                     /locus_tag="VEW91_09905"
     CDS             complement(93..1487)
                     /locus_tag="VEW91_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYM91933.1"
                     /translation="MDGRFEIVSPVDGSIYATLPYADEATVDAAVARARVAQRDWCRT
                     DLELRAERCRAFVHAMEGKRQEIAIELAWLMGRPVRFGPAEVTRLGERATHMIAIAEQ
                     SLAPIEIAAGDGVVRRIRHEPLGVVLVIAPWNYPYLTAVNAIVPALMAGNAVILKHAT
                     QTSPCGNRFAAAFAEADLPDGLFQSVQMSHASIERIISQKKVDFVAFTGSVRGGSEVE
                     RSAAGRFIGVALELGGKDPAYVRPDAPLEATAEALVDGVFFNSGQSCCAVERIYVHEA
                     VYARFVDLFVDTTRATQKLGNPLDRETTLGPVVNAAAAASIRSQIAAAIAAGARPLLS
                     AAEFAAATPDGPYVAPQILTDVDHGMSVMREETFGPAVGIMRVSSDAEAVGLMNDSRY
                     GLTASVWTGSLDEGLSLADRIETGTCFVNRCDYLDPALAWVGVKDSGRGCSLSSLGYA
                     QLTRPKSFYGRALR"
     gene            1824..2354
                     /locus_tag="VEW91_09910"
     CDS             1824..2354
                     /locus_tag="VEW91_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91934.1"
                     /translation="MNRYLAATVCLVLLAWTAQIGSAQGNEWAEYVYADDGFAIFAPG
                     EPFRETGTIRGVGGPVELHYYSIPLGHDSGFMVIHGRLHPDDQRSPPQVLTDAENGTV
                     KGVRGSLIAEMPVSLGDYPGLQIEVEAGKYHSRNRYYVVERTLYQLLSVAPLGRPISP
                     ETDRFFNSFRLVGSSR"
     gene            2478..2732
                     /locus_tag="VEW91_09915"
     CDS             2478..2732
                     /locus_tag="VEW91_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91935.1"
                     /translation="MNEGVIERSGFRIEPQSQWVPSGRGVRKGWRPKAVVWEAGNPRL
                     EYVIAPRDVETFPTKEEADAFMLQWAQRWLERRSLSPALR"
BASE COUNT          472 a          912 c          931 g          453 t
ORIGIN      
        1 acgacctccg cctgccaata cccgcgaacc cgccaccagg ttctgcgcca tcagccctcc
       61 cggcggttac cgtcgagagc aggctcccac gctcagcgca gcgcccggcc gtaaaaggac
      121 ttcggccggg tgagttgggc ataccccagc gaggataggc tgcagccgcg gcccgagtcc
      181 ttgacgccga cccaggccag ggccggatcg agatagtcgc accggttgac gaagcacgtc
      241 ccggtctcga tccgatccgc gagggacaag ccctcatcga ggctgccggt ccacaccgac
      301 gccgtgagac catagcggct gtcgttcatg agcccgacgg cctccgcatc cgaggagacg
      361 cgcatgatcc cgaccgctgg gccgaaggtc tcctcacgca tcacgctcat cccatggtcg
      421 acgtccgtga ggatctgcgg cgccacgtag ggtccgtcgg gcgtcgccgc ggcgaattcg
      481 gccgccgaga gcaggggcct ggccccggcg gcgatggccg cggctatctg cgatcggatg
      541 ctggcggcgg cggcggcgtt gacgaccggg cccagggtgg tttctcggtc gagagggttg
      601 ccgagcttct gcgtcgcccg cgtcgtgtcg acgaaaagat cgacgaagcg ggcgtagacc
      661 gcctcgtgga cgtagatccg ctcgacggcg cagcaggact ggccgctgtt gaagaagacg
      721 ccgtcgacga gggcctccgc ggtggcttcg aggggcgcgt ccggtctcac gtaggctgga
      781 tccttgcccc cgagctccag cgcgacgccg atgaaccggc cggccgccga gcgctccacc
      841 tcgctccccc cgcgcaccga gccggtgaag gccacgaagt ccaccttctt ctgagagatg
      901 atgcgttcga tggacgcgtg cgacatctgc acgctctgga agagcccatc gggcaggtcg
      961 gcctcggcga aggccgcggc gaaacggttt ccacacggcg acgtctgcgt ggcgtgtttc
     1021 agaatcaccg cgttgccggc catgagcgcc ggcacgatgg cgttcacggc ggtcaggtag
     1081 ggatagttcc acggggcgat caccaggacg acgccgaggg gctcgtgccg aatgcggcgg
     1141 accacaccat ccccggcggc gatctcgatt ggcgcgagac tttgctccgc gatcgcgatc
     1201 atgtgcgtgg cgcgctcacc gagccgcgtc acctcggcag ggccgaagcg gaccggacgt
     1261 cccatcagcc acgcgagctc gatcgcgatc tcctggcgtt tcccctccat cgcgtgcacg
     1321 aaggcacggc atcgctccgc tcgcagctcg aggtcggtgc ggcaccagtc ccgctgggcc
     1381 acgcgggcgc gcgcgaccgc cgcatccacg gtggcctcat ccgcatacgg cagggtcgca
     1441 tagatgctgc catcaaccgg ggagacgatc tcgaagcgcc cgtccatttg gatggtcctc
     1501 cgacgctcac aggctccggc tctcacgaaa agtatagcgc aaaccgaccc tgccggcggg
     1561 tggaagagtc ccgtggtggc tgccccgaaa taatggcggc atcgagatgc gcgctgacgc
     1621 gcaggctgtc atcatcggcc agttgcgcgg atcgtacaac cggacccgca tgatcatgta
     1681 cagcgtggat ctctatgggc ggatcggcac cgagacgagc atcgatcccg actggcgcca
     1741 ggtgggaagt ctccgcctcg cgtccgtggg cgtccgatcc agggccccgg tgggcatcac
     1801 aacgttgtgg cgacggtgcg atcatgaacc ggtacctggc tgcaacggta tgtcttgttc
     1861 ttctggcttg gacagcccag atcgggtctg cgcagggcaa cgaatgggcg gaatacgtct
     1921 acgccgacga cggcttcgcc atcttcgctc cgggggaacc ctttcgggag accgggacta
     1981 tccgcggtgt cggcggcccg gtggagcttc attactactc gattcctctg ggccatgatt
     2041 cgggctttat ggtgatccat ggtcggctcc atcccgatga ccagcgttcg ccgccgcagg
     2101 tgctcacgga cgccgagaat ggaacggtca aaggagtgcg gggcagcctc atcgctgaaa
     2161 tgccggtttc cctgggggac tatcccggcc tccagatcga ggtagaagcg gggaaatacc
     2221 actcgcggaa ccgatattac gttgtggaaa gaacgctgta ccagctcctc tccgtggcgc
     2281 cgctggggag gccaatctcc ccggagacgg accgcttttt caactccttc cgcttggtgg
     2341 gtagctcgcg gtagccgacg ttggcacgac cgaatggccg gacgtgcccg ctccccccgg
     2401 tccgagcggc cggtctgtct tagacgtcgc gcattcgggc atacacctat cccaaccagg
     2461 acctgggagg gcataggatg aacgagggcg tgatcgagcg cagcggcttc cgcatcgagc
     2521 cgcagtctca atgggtgccg tccggccgcg gcgtccggaa agggtggagg ccgaaagccg
     2581 tggtgtggga agccgggaat ccccggctcg agtatgtgat tgctccgcgg gatgtcgaga
     2641 catttcccac aaaggaagaa gccgacgcct tcatgctgca gtgggcacag aggtggctgg
     2701 agcggcggag cctatccccg gcgctgcggt agggacgaca gcgccccggg catcacccgt
     2761 cgccgtcg
//
LOCUS       DASQGH010000506         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39080, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000506 DASQGH010000000
VERSION     DASQGH010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39080"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2366
                     /gene="nifJ"
                     /locus_tag="VEW91_09920"
     CDS             <1..2366
                     /gene="nifJ"
                     /locus_tag="VEW91_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HYM91936.1"
                     /translation="GLSLPLGSDADIEAADVTRAVFFGLGADGTVGANKNSIKIIGEE
                     TPNFAQGYFVYDSKKAGGKTISHLRFGPRPMRSAYLIKSANFVACHQFSFMERHDVLR
                     YAAPGAVFLLNAPYGPDEIWSRLTQEVQEQIVRKRLRFYVIDALEVARRTGMGGRINT
                     VMQTCFFAIANVLPRAEAIARIKEAIRETYGRQGEEVVRRNWRAIDETLDNLHEVPVP
                     DQVSATRRRPALIPEEAPDFVKRVTGMFLADQGDVLPVSAFPPDGTWPVGTSRWEKRN
                     IAQEIPVWDPAICIQCNKCVFVCPHAVIRAAVYDPAALAGAPSTFKATDYRGLDFKGQ
                     KYTLQVAPEDCTGCSLCVVTCPVKDKTNPKHKAINMAAQPPLREAERENWAFFLALPE
                     PDRRRLKHDVKESQFLRPLFEFSGACAGCGETPYLKLLTQLTGDRAFIANATGCSSIY
                     GGNLPTTPYATNPDGRGPAWSNSLFEDNAEFGFGFRLAVDQLRVEARALVERLAPQIG
                     SDLAGTLLSADERDESGIAAQRERVALLRQRLSGLAHPDAHRLESIVDYLVRKSVWIV
                     GGDGWAYDIGFGGLDHVLAMGRDVNILVLDTEVYSNTGGQQSKATPRGAAAKFATAGK
                     ATAKKDLALQAMTYGNVYVAHVAFGARDTQTVRAFLEADAYPGPSLIIAYSPCIAHGY
                     DLAYGAEQQKLAVESGYWPLFRYDPRRLAEGKNPLELDSPPPKVDLARFLYNEARFRM
                     VESMDPQRAKKLLAEAREDVLKRYRLYEQLAHLPVPQPASTAAPAGG"
     gene            2379..3395
                     /locus_tag="VEW91_09925"
     CDS             2379..3395
                     /locus_tag="VEW91_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HYM91937.1"
                     /translation="MDLSTTYLGLSLPHPLMPGASPMVDDLDTVKRLEEAGAAAIVMH
                     SLFEEQFLQEQLATVHHIDVHAESFPEALSYFPRPTEFALGPEQYLDQIARIKAAVAV
                     PVIASLNGTTAGGWIDYARRIQEAGAAALELNVYHIATDPQESAGAVERRTLDILRAV
                     RQVMTIPIAVKLSPFFSAMAHFAAELDGAGADGLVLFNRFYQPDIDPESLEAVPSLEL
                     STSSELSLRLQWLAVLSGRVRASLAVTGGIHSARDAIKAVMAGANAVQMVSALLQRGP
                     EYLRVVREEMEAWMDRHEYASLRQMQGSMSLARCPDPAAFERGNYIRILQRWRPEGRS
                     AWTD"
     gene            complement(3408..>5198)
                     /gene="ppsA"
                     /locus_tag="VEW91_09930"
     CDS             complement(3408..>5198)
                     /gene="ppsA"
                     /locus_tag="VEW91_09930"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HYM91938.1"
                     /translation="DVVLITSIWGLGENIVQGRVAPDQFYVHKPTLLQGFKPVLWRKL
                     GAKELRLLYDETEQRLKNLPVPPEQRQQFSISDDEVLQLARWGIAIEAHYSKKRAEAL
                     PMDIEWAKDAATGELFILQARPETVHSQKKGPVVRTYRLKETGKVLAEGLAIGDAIAV
                     GTARVIEDPRGMGAFQAGEVLVTHVTDPDWEPIMKIASAVVTERGGRTSHAAIVAREL
                     GIPAVVGATGATEAIRTGQTVTVSCAEGETGWAYEGALHYETEEVDPTTLPRSRTQIM
                     MNVGTPEQAFSVARLPSDGVGLARMEFIFASWVRVHPLALTRYDTLPVKIQREIDELT
                     RGYTDKREYFVDKLSQGIGTIAAAFWPRPVILRFSDFKTNEYAHLIGGEQFEPREENP
                     MLGWRGASRYYHPDYKEGFALEVAAVKRVRERLGLKNLKVMVPFCRTPEEGRRVLEVM
                     KEGGLVRGDDGLEVYVMAELPSNVILAEQFSEVFDGFSIGSNDLTQLTLGVDRDSERV
                     AALFDERSDAVTRACAELIAKAHQAGRKVGICGQAPSDFPEFAAFLVERGIDSISLNP
                     DAVVATKLRVLEAEKRRDELAATAKALVGD"
BASE COUNT          829 a         1828 c         1676 g          865 t
ORIGIN      
        1 ccggcctctc gctcccgctc ggctccgacg cggacatcga ggccgccgac gtcacgcggg
       61 cggtattctt cggcctgggc gccgacggca cggtgggtgc gaacaagaac tcgatcaaga
      121 tcatcgggga ggagactccc aatttcgctc agggatactt cgtctacgac tccaagaagg
      181 cgggggggaa gacgatttcg cacctgcggt ttggcccccg accgatgcgg tcggcctact
      241 tgatcaagtc cgcgaacttc gtcgcctgcc accagttctc gtttatggag cgccacgacg
      301 tgctccgcta cgcggcgccg ggggccgtct tcttgctcaa cgccccctac ggcccggatg
      361 agatctggag ccggctgacg caggaggtgc aggagcagat tgtccgcaag cgtctgcggt
      421 tctacgtgat cgatgccctg gaggtcgccc ggaggacggg gatgggcggc cggatcaaca
      481 cggtcatgca gacgtgcttc ttcgccatcg ccaacgtgct gccgcgggcc gaggcgatcg
      541 ccaggatcaa ggaggcgatc cgcgagacct acggccgcca gggtgaagaa gttgtacggc
      601 ggaactggcg ggcgatcgat gagacgcttg acaacctgca cgaggtgccg gtgccggatc
      661 aggtgagcgc gacccgacgc cggccggcgc tgatccccga ggaggccccg gacttcgtca
      721 agcgcgtgac cggcatgttt ctggccgatc agggcgacgt gctcccggtc agcgctttcc
      781 ccccggatgg gacgtggccg gtcggcacca gccggtggga gaagcgcaac atcgcgcagg
      841 agatcccggt atgggatccc gcaatctgca tccagtgcaa caagtgcgtc ttcgtctgcc
      901 cccacgcggt catccgggcc gccgtctacg atcccgcggc actggccggc gccccctcca
      961 ccttcaaggc gacggactac cggggcctgg acttcaaggg tcagaagtat acgctgcagg
     1021 tcgcccccga ggactgcacc ggctgcagcc tctgcgtcgt gacatgcccg gtcaaagaca
     1081 agacaaaccc caagcacaag gccattaaca tggccgccca gccgcccctc cgcgaggccg
     1141 aacgcgagaa ctgggcgttc ttcctcgcgc tccccgagcc cgaccgccgc cgtctgaagc
     1201 acgacgtcaa ggagtcgcag ttcctccggc cgctgttcga attctcaggc gcctgcgccg
     1261 gctgcgggga gaccccctac ctcaagctcc tgacccaact caccggcgac cgcgcgttca
     1321 tcgccaatgc cacgggctgc tcctcgatct acggcggtaa cctgccgacg acgccctatg
     1381 ccacgaaccc ggacggccgc gggccggcgt ggtccaactc gttgttcgag gacaacgcgg
     1441 agttcggttt cggcttccgg ctggccgtgg atcagctcag ggtcgaggct cgggcgttgg
     1501 tcgagcggct cgccccgcag atcggctcgg acctggccgg caccctgctg tctgccgacg
     1561 agcgcgatga gtcgggcatc gccgcccagc gcgagcgcgt ggcgctgctg cggcagcgcc
     1621 tctcgggtct cgcgcacccc gacgcgcacc ggctcgagag catcgtggac tatctggtgc
     1681 gcaagagcgt ctggatcgtc ggcggcgacg gctgggccta cgacatcggg ttcggaggcc
     1741 tcgaccacgt cctggcgatg gggcgggacg tcaacatcct ggtcctggac accgaagtgt
     1801 actcgaacac cggcgggcag cagtccaagg ccacgccgcg gggcgccgcg gccaagttcg
     1861 cgacggcagg caaggcgacc gccaaaaagg atctggcgct gcaggccatg acgtacggca
     1921 atgtctacgt cgcgcacgtg gccttcggcg ccagagatac ccagaccgtg cgggcgttcc
     1981 tggaggccga cgcctacccg ggaccgtcct tgatcatcgc ctacagcccg tgcattgccc
     2041 atggttacga cctggcgtac ggggcggagc agcagaaact ggcggtggaa tcgggttact
     2101 ggcccctctt ccgctacgat ccccgtcgcc tagccgaagg gaagaatccg ctggagctcg
     2161 actcgccgcc gccgaaggtc gacctggcgc gctttctcta caatgaagcg cgattccgga
     2221 tggtggagag catggacccc cagcgggcca agaaactcct ggccgaggcc cgcgaggatg
     2281 tgctgaagcg ctaccggctc tacgagcaac tggcgcacct gccggtccct cagccggcct
     2341 cgactgcggc ccctgccggc ggctgaaagg aggctcgcgt ggatctctcc accacgtacc
     2401 tgggcttgtc cctaccgcac ccgctgatgc cgggcgcgtc gccaatggtg gacgacctgg
     2461 acacggtgaa gcgattagag gaggccggcg ccgcggcgat cgtcatgcac tcgctcttcg
     2521 aggagcagtt cctccaggag caactggcca ccgtccacca catcgatgtc cacgccgaat
     2581 cgttccccga ggcgctgtcg tacttcccgc gccccacgga gtttgccctg gggcccgaac
     2641 agtacctcga ccagatcgcg cggatcaagg ccgcggtggc cgtgcccgtg atcgcgtcgt
     2701 tgaacggcac cacggccggt gggtggatcg actacgcgcg gcggatccag gaggccggcg
     2761 cggcggccct cgagctcaac gtctaccaca tcgccaccga cccgcaggag agcgccgggg
     2821 ccgtcgaacg ccgcacgcta gacatcctcc gcgcggtgcg gcaggtgatg accatcccca
     2881 tcgccgtgaa gctgtcgccg ttcttctctg ccatggcgca cttcgcggcc gaactggacg
     2941 gcgcgggcgc agatggcctc gtcctcttca accgcttcta ccagccggat atcgaccccg
     3001 agtctctaga ggcggtcccg tcgttggagc tgtccacgag ttccgagttg tctctccggc
     3061 tgcagtggtt ggccgtcctc tcaggtcgcg tgcgtgcctc actcgccgtc acggggggca
     3121 ttcacagcgc ccgcgatgcg atcaaagcgg tgatggccgg cgcgaacgcc gtgcagatgg
     3181 tgtccgcgct cctgcagcgc gggcccgaat acctgcgggt cgtgcgcgag gagatggaag
     3241 cctggatgga ccggcacgaa tacgcgtccc tgcggcagat gcagggaagc atgagcctcg
     3301 cgcggtgccc cgacccggcc gccttcgagc ggggcaacta catccggatc ctccagcgct
     3361 ggaggcccga agggcggagc gcttggaccg actgagccgc tctgccctca gtccccgacc
     3421 agcgctttgg ccgttgccgc gagctcgtcc cgccgcttct ccgcctcgag gacgcgcagc
     3481 ttggtcgcca cgacggcgtc cgggttgagg ctgatcgagt cgatcccccg ctccaccagg
     3541 aacgcggcga actcgggaaa atccgacggc gcctgcccgc agatcccgac cttccgcccc
     3601 gcctggtgag cctttgcgat gagctccgcg cacgcccgcg tgacggcgtc gctccgctca
     3661 tcgaagagcg ccgcaacccg ctccgagtcc cggtcgacgc cgagcgtcag ctgtgtcagg
     3721 tcgttggacc cgatcgagaa gccgtcgaac acctctgaaa attgctccgc caggatcacg
     3781 ttggagggca gctcggccat gacgtagact tcgagcccgt catcgccgcg cacgagcccg
     3841 ccttctttca tcacctcgag gacgcgccgg ccttcctccg gcgtccggca gaacgggacc
     3901 atcaccttta ggttcttcag cccgagccgt tcccgcacgc gcttcaccgc tgcgacctcg
     3961 agcgcgaacc cttctttgta gtccgggtgg tagtagcggc tggccccccg ccagccgagc
     4021 atcgggttct cctctcgggg ctcgaactgc tccccgccga tcaggtgcgc gtactcattg
     4081 gttttgaaat ccgaaaaccg caggatgacc ggcctcggcc agaacgccgc ggcgatcgtg
     4141 ccgatgccct gggagagctt atcaacgaag tactcccgct tgtcggtgta cccccgcgtc
     4201 agctcgtcga tctcgcgctg gatcttcacc ggaagcgtat cgtagcgcgt cagcgcgagc
     4261 ggatggaccc gtacccagct ggcgaagatg aactccatcc gcgccaggcc cactccgtcg
     4321 ctcggcagcc gggcgacgct gaaggcctgc tccggggtgc cgacgttcat catgatctgc
     4381 gtccgggagc gagggagggt cgtggggtcc acttcctccg tctcatagtg aagcgccccc
     4441 tcgtacgccc agcccgtctc cccctccgcg caggagacgg tgacggtctg gcccgtccga
     4501 atcgcctcgg ttgctcccgt cgccccgacg accgccggga tccccagctc ccgcgcgacg
     4561 atcgccgcgt gcgacgtccg cccgccgcgt tcggtcacca cggccgaggc gatcttcatg
     4621 atcggttccc agtcgggatc ggtgacgtgg gtcactagca cctcgccggc ctgaaaggcc
     4681 cccatgcctc gcgggtcttc gatgacccgg gcggtcccga ccgcgatcgc gtcgccgatc
     4741 gccaggccct cagccagcac cttgccggtt tccttcagcc ggtacgtccg gacgaccggg
     4801 cctttcttct ggctgtgcac cgtctccggc ctggcctgca ggataaagag ttcgcccgtg
     4861 gcggcgtcct tggcccactc gatgtccatc gggagcgctt cggcccgctt cttgctgtaa
     4921 tgggcctcga tggcgatgcc ccaccgcgcc agctggagca cctcgtcgtc gctgatggag
     4981 aactgttgtc gttgctcggg gggaaccgga aggttcttga gacgctgctc ggtctcatcg
     5041 tagagcagcc tcaactcctt ggcgccaagc ttccgccaga ggaccggctt gaagccctgc
     5101 agcagcgtgg gcttgtgcac gtagaattgg tcgggggcca cccggccctg cacgatgttc
     5161 tctccaagcc cccagatcga ggtgatgagc accacgtc
//
LOCUS       DASQGH010000507         7925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55784, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000507 DASQGH010000000
VERSION     DASQGH010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55784"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VEW91_09935"
     CDS             complement(<1..676)
                     /locus_tag="VEW91_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91939.1"
                     /translation="MPSINTNPATRTLDALRAISLVGIGLYAITLAAHWFSAVARYLI
                     LYIVLGLIGLVTGWPIPVNTTATVLALAPLAISLLTLICPPLIAPLDGRWWEISTGGR
                     PPEQDEREAFEDAFHELQEIDPRLRPPRHWFVAEEPGQNASAYSSSLSVDRGLLESPN
                     TAAVIAHELGYLRTSDARLTSALNLLLLAPMDTPTLHPLWSLPFRGLAWFASGQAVQW
                     FTSNPWE"
     gene            complement(676..1014)
                     /locus_tag="VEW91_09940"
     CDS             complement(676..1014)
                     /locus_tag="VEW91_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91940.1"
                     /translation="MREIDNELRKARMEEKEAAQRVKRLLAAQAALRGEPVPRKHQRR
                     LARQDIRDYLAKHPGSLAVEIAEALGVPPTNVGTHLSNGKKDGEFEKHRNRWSLIQHE
                     GGQPQHKETP"
     gene            1197..1544
                     /locus_tag="VEW91_09945"
     CDS             1197..1544
                     /locus_tag="VEW91_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91941.1"
                     /translation="MGGLFALVLVLCAGVFLRAAIGSGSGSVFAWAIGLVVVLVVLRV
                     ALTLGVGALTRTPRTPARPLPRVPGTRGGAVARRPVSAAARVREGLLRVGGGVYLGAN
                     EYGNGSQRTPRAL"
     gene            1544..2779
                     /locus_tag="VEW91_09950"
     CDS             1544..2779
                     /locus_tag="VEW91_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HYM91942.1"
                     /translation="MILGPPRSGKTSAVMIPALIAASGAVVSTSTKPDVMRATMNARM
                     EIGEVWLFDPSASEPVPEGARRLSWSPVSASSSWDEALVMARAMTAASRAGAGTTNEN
                     HWSERAAALLGPLLYAAHLSNRPIEDVLRWTLRQDLAPALEVLADAETQIAADVLVGI
                     ERTDARERSSIFSATAGVLSAYNSDSARQTAARPNFDPVRFAASTDTLYITSPEHGQA
                     VFAPLIVGLLEQIRHAVYEQARNNHAPGPGMLWLLDEVANIAPVHDLPALVSQAGGQG
                     LQVVIGLQDLSQARTRWGVDAADGFLSLFQSKLILNGIADTRTLEAVSVALGEYDRDV
                     TSQALGHSQLQEWFSHPTHSDTVSYQTQRQRVLTPGEIARLPDGHGLLLRGTDWELLR
                     LTRWYDSEPWRTVGAGTTG"
     gene            complement(2834..3745)
                     /locus_tag="VEW91_09955"
     CDS             complement(2834..3745)
                     /locus_tag="VEW91_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HYM91943.1"
                     /translation="MKYTSAAAFRQALEQRLKNEATSTGLGLARLRKQVAFELFLRRL
                     LIVAPDHWVLKGALALNFRLAIATRATKDIDLGRDDDEQAAIGDITAAQQLALDDHFT
                     FAATRTHELDDTDEFSAVRFHVTAQLAGRTFEQFLLDIGFTDTISWTPDIIHTSGSLS
                     FAGIQPLSVPVIPIPQHLAEKVHAYTRTYGETNQPSTRPKDLIDILLIESTTTIQADA
                     LRHALQSTFTERARQPLPVSLPPPPTAWTDPYKRLAVTVRIESDLHAAYTRAAAFLDP
                     ILAGHANGQWDPQHHHWIATEDSPAKP"
     gene            complement(3742..4317)
                     /locus_tag="VEW91_09960"
     CDS             complement(3742..4317)
                     /locus_tag="VEW91_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HYM91944.1"
                     /translation="MLGLEAEAYQQGGYFNAAQARAHAISRQLLEHHVRRGRFERVRR
                     GLYRIQGYPTAEHDDMREAWMTVSATGALLSHESALALLDLSDSIPDAVHLLVPRKHR
                     GLRRPTGVILHTHPDSEAIPTVWRDGLPLTAPARTLLDVASHLQPEQLSMAITQALRR
                     GLLTTEQLQEEAARRHKQDIVQAILAAGTHA"
     gene            complement(4471..5043)
                     /locus_tag="VEW91_09965"
     CDS             complement(4471..5043)
                     /locus_tag="VEW91_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91945.1"
                     /translation="MSGGVGGAGLSPAPTRSGGTSSHAGPCYFPSRAGVERLLHHDSG
                     RDRGLVRRAAYAEIGLAAEKLDTAAFARDREAHPEWFLGPAADLRESYALLDRIGWSK
                     TTPPFAVQIELRDYGWALLRALEGALEFADEDIGDHVGEDVEHAAQNLDRVPDAVVER
                     VGTLWHFTADAQARIDALAVQEGVALTIAA"
     gene            complement(5013..6326)
                     /gene="ltrA"
                     /locus_tag="VEW91_09970"
     CDS             complement(5013..6326)
                     /gene="ltrA"
                     /locus_tag="VEW91_09970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HYM91946.1"
                     /translation="MSLWERFLSRENLALALRRVERNAGAAGINGMSTKELRPWLHSH
                     WPQVRSALDAGTHRPSPVRRVMIPKPSGGRRMLGVPTAVDRLICQALSQVLTPVFDPY
                     FHPNSFGFRPGRSAHQAVERARQFIADGDAGWCVEVDLDSFFDRVCHDALMARVARRV
                     QDKQALKLIRAYLDAGVMADGIVQVTGEGTPQGSPLSPLLSNVMLDDLDWELDRRGHR
                     FVRYADDSRIYVERAGQRVLQSISGYVEHSLKLKVNQGKSAVDRAGRRPFLGFCFHRR
                     KGEVKVWLDPKARKRAKDQIRRLTSRTCGVSMQRRITEINRFTVGWTAYFAFADTPTP
                     FIELDGWLRGRMRQARWKEWQRIRTRWSRPQALGMPDREAREWAMTNKGSWRAARTFL
                     KRALPDAYWAEQGLKGFADPYHRLRDTTRTAGCGPACPVVWEGPG"
     gene            complement(7356..>7925)
                     /locus_tag="VEW91_09975"
     CDS             complement(7356..>7925)
                     /locus_tag="VEW91_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6119 family protein"
                     /protein_id="HYM91947.1"
                     /translation="KRYRTEVEAFVRTIPELNIGLPPAAASDDEYAYNQRAAQAVGAL
                     CLDRKLINTGGPDSVEICDILTKDGIFIHVKKRGRSSTLSHLFTQGINSAELLLHDEI
                     FLDSAREIVGELNRRFVSAIPKTLRAREQMTVAFVVLSRSKRKDTPHGLPFFSLMSLQ
                     AAARHLQDIGIQVQVQQIKEAAPTSQPTP"
BASE COUNT         1196 a         2464 c         2744 g         1521 t
ORIGIN      
        1 tctcccacgg gttgctggtg aaccactgca ccgcttgtcc tgaggcgaac catgcgagac
       61 cgcggaacgg cagcgaccag agggggtgca gggtgggcgt gtccatcggg gcgaggagta
      121 ggaggttcag tgcgctggtg agtcgcgcgt cgcttgtgcg taggtatccg agctcgtgcg
      181 cgatgaccgc ggcggtgttg gggctttcca gcaggcccct gtccacgctc agcgaggagg
      241 agtaagcgga ggcgttctgt ccgggctctt cggcgacgaa ccagtgtcgt ggcgggcgca
      301 gccgggggtc gatctcctgc agttcgtgga aagcgtcctc gaatgcttcg cgctcgtctt
      361 gctcgggtgg gcgtccgccg gtgctgatct cccaccagcg cccgtcgagg ggcgcgatca
      421 gtggtgggca gatcagggtc agcagcgata tcgcgagggg tgcgagggcg agcacggtgg
      481 cggtcgtgtt gaccggtatc ggccagcccg tgaccaagcc gatcagcccc agcacgatgt
      541 agaggatgag gtatcgcgcg actgccgaga accagtgtgc agcgagggtg atcgcgtaca
      601 gtccgatccc gacgaggctg attgcccgca gggcgtcgag tgtccttgtg gctgggtttg
      661 tgttgatgct gggcatcaag gggtctcctt gtgttgcggt tgcccccctt cgtgctggat
      721 caatgaccag cggttacggt gcttttcgaa ttccccatcc tttttgccgt tgctgaggtg
      781 cgtccccacg ttcgtagggg gtacgccgag ggcttcggcg atctccaccg cgagcgagcc
      841 gggatgcttc gcgagatagt cgcggatgtc ttggcgggcg agacggcgct gatgcttgcg
      901 tggcacgggt tccccgcgca gcgcggcctg cgccgcgagc agtcgcttga cgcgctgtgc
      961 ggcctccttt tcctccatcc tggctttgcg tagctcgttg tcgatctcgc gcatcgcgcc
     1021 gagcgcgtac ctgcggggtg gcatcagccg atcctcatag tgttgcttag catgttgagc
     1081 aacactagtg tatactgcca gtcatgtcaa ggaggatcgg tgatcggtcg tcgtggggtc
     1141 cttcgcacca gtacacggac ccgccgtttc ggcgctcgca tccgttcgcg tcgtgcatgg
     1201 ggggattgtt cgcgctggta ctggtgttgt gcgcgggtgt gttcttgcgc gcggcgatcg
     1261 gatccggttc ggggagcgtg ttcgcgtggg cgatcggcct cgtggtcgtc ctggtggtgc
     1321 tgagggtggc gctcacgctc ggcgtcggtg ccctcacccg aactccccga acccccgcgc
     1381 ggccgctccc gcgagttcct ggtacgcgcg gtggcgcggt ggcgcgcagg ccggtgagtg
     1441 cggctgcgcg tgtacgggaa ggactgttgc gggtcggggg aggggtgtat ctcggtgcca
     1501 acgagtacgg gaatgggtcg cagcggaccc cgagagcgct gtgatgatcc tcgggccgcc
     1561 gcgcagcggc aagacgagcg cggtgatgat ccccgcactg atcgctgcct cgggggcggt
     1621 ggtgagcacg tccaccaagc ccgacgtgat gcgcgccacg atgaatgctc gcatggagat
     1681 cggcgaggtc tggctgttcg acccctccgc gagcgagccc gtacccgagg gcgccagacg
     1741 cctgtcgtgg tcgcccgtca gcgcgtcctc ctcgtgggat gaggcgctgg tcatggccag
     1801 ggcgatgacg gccgcctcca gggccggcgc cggcacgacg aacgagaacc actggtccga
     1861 gcgcgccgcc gcgctcttgg gaccgttgct gtatgccgcg cacctgagca accgtccgat
     1921 cgaggacgtg ctgcgctgga cgttgcgcca agacctcgcg ccggccctgg aggtcctcgc
     1981 ggacgccgag acacagatcg cagcggatgt tctggtcggg atcgagcgca cggacgcgcg
     2041 ggagcgctcg agcatcttct ccgccacagc cggggtgctc agcgcgtata acagcgacag
     2101 cgccaggcag accgcggcgc gcccgaactt cgacccggtc aggttcgccg cctcgacgga
     2161 cacgctgtac atcacctctc ccgagcacgg tcaggccgtg ttcgcaccgt tgatcgtggg
     2221 gctcttggag cagatccgtc acgccgtcta cgagcaggcc cgcaacaatc atgctcccgg
     2281 acccggaatg ttgtggttgc tggatgaggt cgcgaacatc gctcccgtcc acgatctccc
     2341 cgcgctcgtc tcccaggccg ggggacaggg gttgcaggtc gtgatcggcc tgcaggacct
     2401 ctcccaagca cgcacccgct ggggtgtgga cgcggccgac gggttcctct ccctgtttca
     2461 gtcaaagctc atcctcaacg gcatcgccga cacccgcacc ctggaggccg tctctgtcgc
     2521 gctgggggaa tacgaccgtg acgtgaccag tcaggcgctc ggtcacagcc aactgcagga
     2581 gtggttcagc cacccgaccc atagcgacac cgtgagctat cagacccagc gccagcgtgt
     2641 cctcacaccc ggtgagatcg cgcggctccc cgacgggcac gggttgctgt tgcgcggcac
     2701 cgactgggag ctcttgcggc tcacacgctg gtatgacagc gagccgtggc ggaccgtcgg
     2761 cgcgggcacg acagggtagg cggtgtgtgg ctcgatgtgg cgcggcgcgc ggtgattcgg
     2821 ccggggcagc cgttcagggt ttcgctgggc tgtcttctgt cgcaatccag tggtggtgtt
     2881 gagggtccca ttgtccgttg gcgtgtccgg cgaggatggg gtcgaggaag gcggcggcgc
     2941 gggtgtaggc ggcgtgtagg tcgctctcga tccgaactgt gacggcgagg cgtttgtagg
     3001 ggtcggtcca ggcggtgggg ggtggtggga ggctcacggg taggggttga cgggcgcgct
     3061 ctgtgaaggt gctctgcagg gcgtggcgca gcgcgtcggc ttggatcgtg gtcgtgcttt
     3121 cgatcaggag gatgtcgatt agatccttcg gtcgggtgct gggctggttg gtttcaccgt
     3181 aggtgcgggt gtaggcgtgc accttctccg ccaggtgttg tgggatcggg attacgggaa
     3241 cagagagcgg ttggataccg gcgaatgaga gcgaccctga cgtgtggatg atgtcggggg
     3301 tccaggagat cgtgtcggtg aagccgatgt ccaggaggaa ctgctcgaag gtgcgcccgg
     3361 cgagctgtgc tgtgacgtgg aaacgcacgg cggagaactc gtcggtgtcg tccagctcgt
     3421 gggtcctggt ggcggcgaac gtgaagtggt cgtccagcgc gagctgttgc gcggcggtga
     3481 tgtcaccgat cgcggcctgc tcgtcgtcgt cgcgtccgag gtcgatgtcc ttcgttgcgc
     3541 gggtggcgat ggcgagacgg aagttcagtg cgagtgcgcc cttgagcacc cagtggtcgg
     3601 gggccacgat cagcaggcgg cgcaggaaca gctcgaacgc gacctgcttg cgcagacggg
     3661 ccagcccgag gccggtgctg gtggcttcgt tcttcaggcg ctgctcaagc gcttggcgga
     3721 acgctgcggc gcttgtgtac ttcacgcgtg tgtcccggcg gcgaggatgg cttgaactat
     3781 gtcctgtttg tggcggcgag cggcttcctc ttgcagttgc tcggtggtga gcagtccgcg
     3841 tcgtagtgcc tgtgtgatcg ccatcgagag ctgctcgggt tgtaggtgcg acgcgacgtc
     3901 gagcagggtg cgtgctgggg cggtcagtgg cagcccgtcg cgccagacgg tcgggatcgc
     3961 ttcgctgtcg gggtgggtgt ggagaatgac gcctgtgggt cggcgtagtc cgcggtgttt
     4021 gcgcgggacg agcaggtgca cggcgtcggg aatgctgtcg ctgaggtcca gcagcgcgag
     4081 cgcgctctcg tgggaaagga gcgcgccggt ggcgctaacg gtcatccatg cctcgcgcat
     4141 atcatcgtgc tcggcggtcg ggtatccctg tatgcgatac agcccgcgac ggacacgctc
     4201 gaagcgcccc cggcgtacgt ggtgctccag gagctggcgg cttatcgcgt gcgcgcgggc
     4261 ctgggcggcg ttgaagtagc cgccctgctg gtatgcctcg gcctccagcc cgagcacatc
     4321 aggatgatga gtgtccgaca taagacaagt atagcttgac tttcgcagac tcataagggc
     4381 tggtgggtct ctcggatgat ccgagagacc cacggcgcgg cggccgtcga gttacgggat
     4441 gcgcgagcgg cctgagcggt gggcggacgc tcaggccgcg atggtcagag caacgccctc
     4501 ttgcacggcg agcgcgtcga tgcgcgcctg cgcgtcggcg gtgaagtgcc acagtgtccc
     4561 cacgcgctcc accacggcgt cgggtacccg atccagattc tgcgcggcgt gctcgacatc
     4621 ctcgccgacg tggtcgccga tgtcctcgtc ggcgaactcc agggcaccct ccagcgctcg
     4681 cagcaatgcc cagccgtagt cgcgcagctc gatctgcacg gcgaagggcg gggtcgtctt
     4741 tgaccagccg atcctgtcga gcagggcgta gctttcgcgc aggtccgccg cgggtccgag
     4801 gaaccactcg gggtgcgctt cgcgatcgcg cgcgaacgcg gccgtgtcga gcttttcggc
     4861 tgccagtccg atctcggcgt atgcggcgcg gcgtaccagt ccacgatcac ggccggaatc
     4921 gtgatgcaga agccgttcga cgcctgcgcg tgaggggaaa taacatggtc ctgcatggga
     4981 gcttgtacct cctgatcggg tcggggccgg gcttaacccg gcccctccca caccaccgga
     5041 catgcgggtc cgcatccggc ggttcgcgtc gtgtcccgga gacggtggta agggtcggcg
     5101 aatcccttca ggccctgctc ggcccagtag gcgtctggca gggcgcgctt gaggaaggtg
     5161 cgtgctgcac gccaggagcc cttgttggtc atggcccact cgcgggcctc cctgtcgggc
     5221 attcccagcg cttgcgggcg gctccatcgt gttctgatgc gctgccattc cttccagcgt
     5281 gcctgtctca tcctgcctct gagccagccg tcgagctcga taaacggcgt gggtgtgtcg
     5341 gcgaacgcga agtaggccgt ccacccaacc gtgaagcggt tgatctcagt gatcctccgc
     5401 tgcatcgaga caccacaagt acgggaggtc aaacggcgta tctggtcctt ggcccgcttg
     5461 cgggccttgg ggtccagcca caccttgacc tcgcctttgc ggcggtgaaa gcagaacccc
     5521 agaaaagggc gccttcccgc ccggtccacc gcggacttcc cttggttgac cttcaacttg
     5581 aggctgtgtt cgacgtatcc gctgatgctc tgcaggacgc gctgtcccgc ccgctcgaca
     5641 tagatcctgc tgtcgtccgc gtagcgcaca aaacggtgcc cgcgccgatc aagctcccag
     5701 tcgaggtcgt cgagcatgac gtttgagagt agcggcgaga gcggggagcc ctgtggggtc
     5761 ccctctcccg tcacctgcac gatcccatca gccatgacac ccgcgtccag gtaagcccgg
     5821 atcagtttga gcgcctgctt gtcctgcacc cgtcttgcga cccgtgccat cagagcgtcg
     5881 tggcagaccc gatcgaagaa cgagtcgaga tccacctcga cgcaccaccc cgcgtcacca
     5941 tccgcgatga actggcgtgc ccgctcgacc gcttgatggg ccgagcgccc cgggcgaaac
     6001 ccgaagctat tgggatggaa atacggatcg aagaccggcg tgagcacctg cgatagggcc
     6061 tggcagatca accgatccac cgccgtcggt acccccagca tccgtcgtcc gcctgagggc
     6121 ttggggatca tcaccctcct gacaggcgac ggccggtgag tacccgcgtc gagcgctgag
     6181 cggacctgcg gccaatggct gtggagccac ggccgaagct ccttggtgct catcccgttg
     6241 atgcctgcgg caccggcgtt ccgctcgaca cgtctgagcg ccagagcaag gttctcgcgg
     6301 gagagaaacc gctcccacag actcaccccg gcggggtgag gtgcggtgtc gtgtggcgcc
     6361 gaggatgggc tgtgctctgg ccgggcgctc tcgggcttca ccccaagctc tcccgaccag
     6421 gccccatgcg gggttggctg ccttcaacgt ccatcccacg agcacgcacg tcccgcgcct
     6481 cgacttcgcg ttcggccctt cccgacgacc atcgaccccg ccggtactat ggcctctgct
     6541 gacttctcca cgacgagcat cggcctctcg accggcaccg tccccaccca gcgaccacgc
     6601 caagcgtgac cgggcatcac gggacacccg cggagatctc cccaggtaag accagcaacc
     6661 ttcaccacac acccgccgca tctacgtctc ggctccttga tgacatcggg cttcgccatc
     6721 tacagctggc tcgcccggac cgcgccgcct cgaatgcgat tcgtgtacct cagggcgcag
     6781 cttcgcctcc agcttcctcc ccaccccgcc tcacgacgac gcagttgcct tcgacttgtg
     6841 gttggtgcca tcaaccccca caggggactc gcacccccga gctgctggtc atgctgagcg
     6901 cactgtcatg accccggctg tttgcgcagc gcgggggtca aatctttgtt gttgaccccc
     6961 tattttagtg gtgttgtggt ggaagtcaac attattgggt gaatctggcg cgtttgcgac
     7021 ctcgggttcg cgcgagggtt ttagttaatc gctttgctcc atgtcgtggt cgcgggcgtt
     7081 caaggtgagg ctgagccgtg gtcaaggccg tcccgaaggg ccgccccgca ggggcggagc
     7141 gccttgacca cggcgggcgc ccgcaccctc cacccagccc atcatcttct cagtcggcag
     7201 ctccgctgcc gacatgaccg gctaactttc gcgttcgcgc cctcagcggg cttcgttcgc
     7261 catcggcgtt cctgctcaca tggagcacgc gggggagtcg cgtcagcgac ggtgcgcgcc
     7321 tctgtggcgc agccggctgt gcccaagcaa atcgctcaag gcgttggttg agatgtcggt
     7381 gcggcctctt taatctgctg aacctgcact tggatgccaa tgtcttgcag atgccgagcg
     7441 gcagcttgca ggctcatcag actaaaaaaa ggcaggccgt gtggcgtgtc tttacgtttg
     7501 ctccgactca agacgacgaa ggcgacggtc atctgttcac gggctcggag ggttttcgga
     7561 atcgcggaga cgaagcggcg gttgagctca ccgacgattt ctcttgcgga gtccaggaat
     7621 atctcatcgt gaagaagcag ttcggcggag ttgatgccct gcgtgaacag gtgactcaaa
     7681 gtggagctgc gaccgcgctt cttgacgtgg ataaaaatcc catccttggt gaggatgtcg
     7741 cagatctcca cactgtctgg gccgcccgtg ttgataagct ttcggtcaag acaaagcgct
     7801 ccgaccgcct gtgctgcccg ctggttgtat gcatactcgt cgtcgctggc agcggcgggg
     7861 ggcaaaccga tgttgagttc cgggatcgtg cggacgaaag cttcgacctc tgtccggtag
     7921 cgctt
//
LOCUS       DASQGH010000508         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5069, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000508 DASQGH010000000
VERSION     DASQGH010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5069"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VEW91_09980"
     CDS             complement(<1..340)
                     /locus_tag="VEW91_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91948.1"
                     /translation="MKPFALASPVAVATVLVDGIRDRTLLGHLGISLARLVVSMTVAG
                     TAGIALGVLVGISRPLALFVEPLAGFFNALSGIVWLPLAITWFGLTWMTVLFVIGNGI
                     FFTVFFNTLVG"
     gene            complement(475..1275)
                     /locus_tag="VEW91_09985"
     CDS             complement(475..1275)
                     /locus_tag="VEW91_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91949.1"
                     /translation="MSARRGWAAGTMRAAAGAGQALAPFVLFTAAWALLVAVARWPED
                     VLPSPFAVGRAFGDIIAKGILPVYAGETVRRVLVAAVTGVAIGIVAGFVMGLHDVSAS
                     LLIPPLRFFQSLSGIAWLPLFLIWFGFSDRTIVVAVWYTLVFPVAFNTMLGVRTVPRL
                     YRYAVLTLGGNWRQVVFQVLLPGAIPYIVGGIRLGIAYGWRALIAAEIVIGTGGLGYL
                     IFKAETFHLTARIIAGMTVIGVLWYVTDYFFLRPLEEATIERWGLVYR"
     gene            complement(1272..2081)
                     /locus_tag="VEW91_09990"
     CDS             complement(1272..2081)
                     /locus_tag="VEW91_09990"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM91950.1"
                     /translation="MQAKISIENIVHEYFNPNTWQRVRALDGLSLDVPRGEFVCLVGP
                     SGCGKSTLLYLVAGLTRPTGGRILLDGREVVRPGRDRGMVFQEFAILPWRTVEGNIGH
                     GLEIAGTPRRIKEETVGRYVALMGLNGFEHKYPHELSGGMRQRVAVARTLAADPEVVL
                     MDEPFASLDAQTRNVMQEELVRIALHERKTIVFVTHSVDEAVILGDRVVVLTHRPGRV
                     KASLPIEISREHRRWDVLGAEERFVRYRQEILKMIREEWDAQEPAAEVAPA"
     gene            complement(2090..>2356)
                     /locus_tag="VEW91_09995"
     CDS             complement(2090..>2356)
                     /locus_tag="VEW91_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91951.1"
                     /translation="HQGEAAQIATRWIEGLDEVSARKAITYMNFDPRFSRNTLAAAEL
                     EQRALLAAGRIKQPVDFSQGLNMIFVEKAIRDAPQFFVDLKPVR"
BASE COUNT          417 a          851 c          780 g          308 t
ORIGIN      
        1 cgcccacgag cgtgttgaag aagacggtga agaagatacc gttcccgatc acgaacagga
       61 cggtcatcca ggtgagcccg aaccaggtga tggcgagggg cagccacacg atccccgaga
      121 gggcgttgaa gaatcccgcg agcggttcca cgaacagcgc cagagggcgg ctgatcccca
      181 ccagcacgcc cagggcgatg ccggcggtcc cggcgacggt catgctcacc accagcctcg
      241 ccaggctgat gccgaggtgt ccaagcaggg tccggtcccg aatcccgtcg accaggacgg
      301 tcgcgaccgc cacgggagag gcgagtgcga acggcttcac ccacccactc gccgtcagcg
      361 cgtaccagac ggcgaagagg gcaccaaagg agatcatccc acggacgagc ggcagcccgg
      421 cccacgcggc ccagaagggg cgcgacctgg cggggggccc cgcccggggc ggggctaccg
      481 gtagaccagt ccccatcgct cgatcgtcgc ctcctccagc gggcggagga agaagtagtc
      541 ggtcacatac cagagaacgc cgatcacggt catcccggcg atgatccggg ccgtgaggtg
      601 aaacgtctcg gccttgaaga tcaggtaccc gaggcccccg gtcccgatga cgatctccgc
      661 cgcgatgagg gcgcgccacc cgtaggcgat ccccaaccgg atgcccccga cgatgtaggg
      721 aatcgcgccg ggcagcagca cctggaacac cacctgccgc cagttgcccc ccagggtcag
      781 gaccgcgtac cggtacaacc gcggcaccgt ccgcacgccc agcatggtgt tgaatgcgac
      841 ggggaagacg agcgtgtacc agacggccac gacgatcgtc ctgtcggaaa agccgaacca
      901 gatcaggaac aaggggagcc aggcgatccc cgagagcgac tggaagaacc gcagcggcgg
      961 gatcagcagg ctggcgctca catcgtggag gcccatgacg aatcccgcca cgatcccgat
     1021 cgccacgccc gtcaccgcgg ccacgaggac ccggcggacg gtctccccgg cgtagaccgg
     1081 caggatgccc ttcgcgatga tgtcgccgaa cgcccgcccc accgcgaagg gcgacggcag
     1141 cacgtcctcg ggccaccgag cgacggcgac caggagggcc cacgcggcgg tgaagagcac
     1201 gaacggggcg agcgcctgcc ccgccccggc cgccgcccgc atcgttcccg cggcccaccc
     1261 ccggcgggcg ctcacgccgg cgccacctcg gcggcgggct cctgggcatc ccactcttcc
     1321 cggatcattt tcaggatctc ctgccggtac cggacgaacc gctcctcggc cccgaggacg
     1381 tcccaccggc ggtgctcccg ggagatctcg atcggcaggg acgccttcac ccgtccgggg
     1441 cggtgggtca gcacgacgac ccggtcgccg aggatcaccg cctcgtcaac gctgtgggtg
     1501 acgaacacaa tggtcttccg ctcgtgcagc gcgatgcgga cgagctcctc ctgcatcacg
     1561 ttccgggtct gggcgtcgag ggaggcaaac ggctcgtcca tcaggacgac ctcggggtcg
     1621 gcggcgagcg tccgggccac ggccacgcgc tggcgcatgc ccccggagag ttcatggggg
     1681 tacttgtgct cgaacccgtt cagccccatc aacgcgacgt accgcccgac cgtctcttcc
     1741 ttgatccggc gcggggtgcc cgcgatctcg aggccgtgcc cgatgttccc ctccacggtc
     1801 cgccacggga ggatggcaaa ctcctgaaac accatccccc ggtcccggcc cggccgcacc
     1861 acctcgcgtc catccaagag gatccggccg cctgtcgggc gcgtgagccc ggccacgaga
     1921 tagagcagcg tcgacttgcc gcagccgctc gggccgacga ggcagacgaa ctccccccgc
     1981 gggacatcga gcgagaggcc gtccagggcg cgcacccgct gccacgtgtt cgggttgaag
     2041 tactcgtgga cgatgttctc gatgctgatc ttcgcctgca ccgccgcccc tagcgcaccg
     2101 gcttgaggtc gacaaagaac tggggcgcgt cgcggatcgc cttctcgacg aagatcatgt
     2161 tgagtccctg cgagaagtcg acgggctgct tgatccgccc ggcggcgagc aaggcgcgct
     2221 gttccagctc ggccgccgcg agcgtgttgc ggctgaaccg gggatcgaag ttcatgtacg
     2281 tgatggcttt cctggccgag acctcgtcga gcccctcgat ccaccgcgtc gcgatctgcg
     2341 ccgcctcgcc ctggtg
//
LOCUS       DASQGH010000509         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33415, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000509 DASQGH010000000
VERSION     DASQGH010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33415"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1505)
                     /locus_tag="VEW91_10000"
     CDS             complement(60..1505)
                     /locus_tag="VEW91_10000"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HYM91952.1"
                     /translation="MVIVTPQEGKKDIDPRGAPPRATPPGDGTVGDGKRAERTERSVD
                     LGLQTLRPRWRTRRIVFLALAAILLLLIVAGIGYWRLNAGLVKTDNAQTSGDLAPVSA
                     QITGTVARIDITDNQFVKAGAVLVELDPTDYRLALDQAKANLAAVQAQVQVAQATLAD
                     QENQYRTGVSAARGALAATTPRLPQAQAQVRMDEQTTAAQVAQAQAGVATAQANVQSA
                     QANEETARKTRDRDRQLLTQGAIAQQQVDTDTAAYAAALAQYRAAQEGLRQAQATLAS
                     AEANREQVVISQHAVAVNEGQIAQARAQFEQAMAGDTLIRERMQQLAAAEAQAAAAAQ
                     MVKTAQVNLDRTLIRAPADGWVTNRTVQIGQVVQPNHPMLSITIAQHLWVVANIKETQ
                     LANVRVGDPVRITVDVFRGRVFHGHVISITAASGSTTALLPPDNATGNFIKVVQLVAV
                     RIALDPSTDPNVHLPVGVSAEVTIDTRHRGR"
     gene            complement(1574..3175)
                     /locus_tag="VEW91_10005"
     CDS             complement(1574..3175)
                     /locus_tag="VEW91_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91953.1"
                     /translation="MIHGAWFPRLGVLALACLMLLLAAGRAAAPPAPGGVEVLMFHGD
                     PGRTGWNAAESVLTPASVGSGAFGSLWTSRVEGEIYAEPLAARDIPAGGGTRTIVYAV
                     TSRDLVYAFDAAGGGRVWGPVSLGTPVRRASLSCGNVDPVGITSTPVIDRASSTLYVV
                     GLTTPDEGRTKVYKMVALDLATGAMRNGWPVVIAPPPASGLRFDPAVQQQRGALTLVH
                     GVVYVPFGGYFGDCGGYHGWVVGLPASDPTRQQAFATPTRRMGGIWAAGGIAADGAGN
                     LYAATGNSDSEGPVDLGDGVVRLATTPTLSFSGRPSDFFVPSNFVELNGTDTDLGSSA
                     PLVLPPQRGSSTPDLLFIAGKQGVGYLVNRANMGGVGRGDGVRGEGIYSRCVFGTCRD
                     GPPEVFSATAYWDGGEGARLILVPGRGSQPAPCRGTGGVVALRLEVAPGTGAQTFGVA
                     WCGPSMRDPGAPAVSGSGQDGAVWVVDAGSGILYALRASTGEKVYESAPQDAAGNFHR
                     FVTPAVANGRVYVGAIHAVIAFGLR"
     gene            3270..4148
                     /locus_tag="VEW91_10010"
     CDS             3270..4148
                     /locus_tag="VEW91_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HYM91954.1"
                     /translation="MRHYSLERSVGHWLRRQALMLGRAIHSFWRHGDLFSGAAITFYA
                     LFSLLPLTLLLLVGLQFIFSAERVTRNLGRLFGMTDDLILRTVRAAYAQQGSFGWLET
                     ITLILAAAGVFGAVQVALDRIWGSQGRIFHVRFFVGILTMAASLLIFLGLLVTTTVAF
                     RLIRTSVVGEWLGWPLRPPPGRSSAPGVAAALAQLGIFWVAYRLLPSVPIRWRDALPG
                     ALLAAVVWQVITRVLAWYLGAVADYATLYHSLGAIMALVAWVYGLTCSFLLGAEFIAQ
                     HTPGPSGHEPGPRRSP"
BASE COUNT          602 a         1525 c         1444 g          693 t
ORIGIN      
        1 cccgcgcggc ccgcgaccgc cccgagcact gtcgcgctca tggagaccgc ccgcgagcct
       61 taccggcctc tgtgccgcgt gtcgatcgtc acctcggcgg agacgcccac cgggagatgg
      121 acgttgggat cggtgctggg atccagcgcg atcctcacgg ctacgagctg gacgaccttg
      181 atgaagttgc ccgtggcgtt gtccggcggg aggagcgcgg tggtcgagcc ggaggcagcc
      241 gtgatgctga tgacgtgccc gtgaaacacc ctgccccgga acacgtccac cgtgatgcgc
      301 accgggtctc ccacccgcac gttggcgagc tgggtctcct tgatgttggc cacgacccac
      361 aggtgctgcg cgatcgtgat ggacaacatc ggatgattcg gctgcaccac ctggccgatc
      421 tgcaccgtgc ggttggtcac ccacccgtcc gcgggagccc gaatgagggt ccgatccaga
      481 ttcacctgcg ccgtcttcac catctgggcg gccgccgcgg cctgcgcctc cgcggccgcg
      541 agttgctgca tccgctcgcg gatcagcgtg tctccggcca tcgcctgctc gaactgggcc
      601 cgcgcttgag cgatttgccc ctcgttgacg gcgacggcgt gctgggagat gaccacctgc
      661 tcccggttcg cctcggccga cgcgagggtg gcctgcgcct gccgcagacc ctcctgggcg
      721 gcccggtact gcgcgagcgc ggccgcgtag gccgcggtat ccgtatccac ttgttgctgg
      781 gcgatcgccc cctgggtcag gagctgccga tctcgatccc gcgtcttgcg ggcggtttcc
      841 tcgttggcct gcgccgactg cacgttggcc tgggccgtgg cgacgccggc ctgcgcctgg
      901 gccacctgcg cggcggtggt ctgctcgtcc atccggacct gcgcctgggc ctggggcagg
      961 cgcggcgtgg tagccgcgag cgccccacgc gccgcgctga ccccggtgcg gtactggttt
     1021 tcctggtccg cgagcgtcgc ctgcgccacc tggacctgcg cttggacggc tgcgaggttc
     1081 gccttcgcct ggtcgagcgc gagccggtag tccgtcgggt cgagctcgac cagcaccgcc
     1141 cccgccttca cgaactggtt gtcggtgata tcgatccggg ccaccgttcc ggtgatctgg
     1201 gcgctcaccg gagccaggtc gcccgaggtc tgggcgttgt ccgtcttgac gagccctgcg
     1261 ttcagccgcc agtacccaat cccggccacg ataaggagca gcagaatcgc ggcaagcgcg
     1321 aggaacacga tccgccgggt ccgccaccgg ggccggaggg tctgaaggcc caggtccacg
     1381 gaacgctcgg tccgctccgc cctcttgcca tcccccacgg tcccgtcgcc tgggggggtg
     1441 gcgcggggcg gtgcaccgcg cgggtcgatg tccttcttcc cttcctgagg ggtcacaatc
     1501 accatgatat caaactctta cgtgaatgtt atattccggg gcgcgcaggg gtcggcccgg
     1561 acatcatgcc cggttatcgg agaccgaatg caatcaccgc atgaatcgcc ccgacgtaca
     1621 ctcgtccgtt cgcgaccgca ggagtgacaa atcgatggaa gtttccagca gcgtcctggg
     1681 gagcggattc gtagaccttc tcgccggtgg atgcgcgaag ggcgtagagg atcccggatc
     1741 cggcatccac aacccagacc gcgccatcct gacccgagcc cgagaccgcc ggggcccccg
     1801 ggtcgcgcat cgacgggccg caccacgcca ccccgaaggt ctgagccccc gtccccggcg
     1861 cgacctcgag gcggagcgcg acgacgcccc ccgtgccgcg acacggcgcg ggctggctgc
     1921 cgcggccggg caccaggatc aggcgtgcgc cctccccgcc gtcccagtac gcggtggcgg
     1981 aaaatacttc gggcgggccg tcccggcagg tgccgaagac gcaccggctg tagatcccct
     2041 ctcccctgac gccgtcgccg cggcccaccc cgcccatatt ggcgcggttc acgaggtacc
     2101 ccaccccctg cttccccgcg atgaagagga gatcaggggt cgaggaaccg cgctgcggcg
     2161 gcagcaccag gggcgccgat gatcccagat cggtatcggt accgttgagc tccacgaaat
     2221 tgctggggac gaagaaatcg gagggacgtc cggagaacga cagcgtgggc gttgtcgcaa
     2281 gccggacgac gccgtcgccg agatcgaccg gcccctcgga atcgctgttc ccggtcgccg
     2341 cgtagaggtt gcccgcgccg tccgcagcga ttcccccggc cgcccagatc cctcccattc
     2401 gccgggtggg ggtggcaaac gcctgctgcc gggtcggatc tgaggcgggt aggcccacga
     2461 cccacccatg atagccgccg cagtccccga aatatccgcc gaacggaacg tacacgaccc
     2521 cgtgcaccag ggtgagtgct ccccgctgct gctggaccgc gggatcgaac cgcagccccg
     2581 aggccggtgg gggtgcgatc acgacgggcc acccgttgcg catggcgccg gtcgcgagat
     2641 cgagggccac catcttgtac accttcgtcc tgccctcgtc gggcgtggtg aggccgacga
     2701 cgtagagcgt ggaggacgct cgatcgatca cgggggtgct ggtgatcccc accggatcga
     2761 cgtttccgca ggacaggctc gcccgcctga ccggggtccc cagcgacacc ggcccccaca
     2821 cgcgcccgcc cccggccgcg tcaaacgcgt agacgaggtc gcggcttgtg acggcgtaga
     2881 ctatcgtgcg ggtccccccg cccgccggga tgtcgcgggc ggcgagcggc tcggcgtaga
     2941 tctccccctc gacccggctc gtccacaggc tcccgaatgc tcccgacccg accgaggccg
     3001 gggtgaggac cgactctgcg gcgttccacc ccgtgcggcc gggatccccg tgaaacatga
     3061 ggacctcgac gccgccggga gccggcgggg cagccgcgcg gccggctgcg aggagcagca
     3121 tcagacacgc gagcgccagc acgccaaggc gagggaacca tgcgccgtgg atcattcgct
     3181 tacctctcca gaccgtcgga cgtgggagcc tgccccgatc tataatgaag gaacgcaggc
     3241 ccaaccacaa ttcctctaag gagctggcgg tgcgccacta ctccctcgag cgctccgtgg
     3301 gtcactggct gaggcgccag gcgctgatgc tcggccgcgc catccacagt ttctggcggc
     3361 atggagatct tttttcgggg gcggcgatca ccttctacgc gctgttctcg ctcctcccgc
     3421 tgacgttgct gctgctggtg ggactccagt tcatcttctc ggccgagcgg gtgacgcgca
     3481 acctggggcg cctcttcggc atgacggacg acctcattct ccggaccgtc cgggcggcct
     3541 acgcgcagca aggctcgttc gggtggctcg aaacgatcac cctcatcctt gcagccgccg
     3601 gcgtgtttgg ggccgtccag gtggccctcg accgcatctg ggggtcccag ggccgcatct
     3661 tccacgtcag gttcttcgtc gggatcctca cgatggcagc ctcgctcttg atcttcctcg
     3721 ggttgctcgt gacgaccacc gtggcgttcc ggctgatccg aaccagcgtg gtcggggagt
     3781 ggctgggctg gccgctgcgg cctccccccg gacgcagcag cgcgccgggg gtcgcggcgg
     3841 cgctggctca gttaggcatc ttctgggtgg cgtaccggct tctgcccagc gtgccgatcc
     3901 ggtggcgcga cgcgttgcca ggtgccctcc tggccgccgt ggtgtggcag gtgatcaccc
     3961 gggtgctcgc ctggtacttg ggggcggtgg cggactacgc gaccctctac cactcgctcg
     4021 gcgccatcat ggcgctcgtc gcctgggtgt atgggctgac gtgcagcttc cttcttggag
     4081 ccgagttcat cgcccagcac acccctggac cgtccggcca cgagcctggc ccgcggaggt
     4141 ctccgtaggg agcccggagg gcgcgcaccc tcaggcgggc gatttgacgg ccttgacccc
     4201 gtggtagacc ggcaggagcc agcgccggta ctgcggcacc cggccctcca ccacggagaa
     4261 gtag
//
LOCUS       DASQGH010000510         6002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27628, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000510 DASQGH010000000
VERSION     DASQGH010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27628"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VEW91_10015"
     CDS             complement(<1..937)
                     /locus_tag="VEW91_10015"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYM91955.1"
                     /translation="MTPLVITPGRPLRGVLRVPGDKSISHRAAILGAIARGPTRVFGF
                     LRAEDCLSTLRCLRALGVAIREQGDGLEIQGGPLQEPGDVLDVGNSGTTLRLLAGVLA
                     GHRFHSVLTGDASIRRRPMDRVADPLRRMGARISGREGGRLAPLAIEGGGLQGIPYRT
                     PVASAQVKSAVLLAGLFAEGETSVEEPAQSRDHTERMLGAFGGAVARDGLTTRLRGPA
                     TLRGAEVRVPGDLSSAAFFLVAAALVPGSELAVAGVGLNPTRTGVLDILRMMGARVDV
                     RDVREEGGEPVGTVDVRATRLRGAAIGGALIPRAID"
     gene            complement(934..1305)
                     /gene="aroH"
                     /locus_tag="VEW91_10020"
     CDS             complement(934..1305)
                     /gene="aroH"
                     /locus_tag="VEW91_10020"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HYM91956.1"
                     /translation="MRFRGIRGAVTVDANTEKAILDATQELLQEMVKANEVDADDIAG
                     ILFTVTPDLNATFPAEAGRRLRGWAHVPMMCAQEVPVPSALQRCVRILMFVNTTETAS
                     EVRHIYLRGAERLRPDLTSRS"
     gene            complement(1358..1855)
                     /gene="aroQ"
                     /locus_tag="VEW91_10025"
     CDS             complement(1358..1855)
                     /gene="aroQ"
                     /locus_tag="VEW91_10025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HYM91957.1"
                     /translation="MARVLVIHGPNLNLLGEREPSVYGTITLAEIDRRLQALAREAGA
                     TVETFQSNHEGAIIDRLHAARGRYGAIVLNPGALTHYSYALRDAIASIAVPVVEVHVS
                     NIHAREAFRHTSVIAPVAKGQIIGFGVHSYLLGLRAALALAEAPPRPTPAPRAGRAPR
                     PRRKA"
     gene            complement(1848..3503)
                     /gene="aroB"
                     /locus_tag="VEW91_10030"
     CDS             complement(1848..3503)
                     /gene="aroB"
                     /locus_tag="VEW91_10030"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HYM91958.1"
                     /translation="MRPNIVLIGFMGTGKTAVGELVASRLGRPFLDTDAIIERLAGRP
                     IPRIFAEDGEAAFRRLEAQAVAEATARGGSVIATGGGVVLSRENMDRLRRDGLIVALH
                     ADPQSILARVGGEGTRPLLGDDPEGSVRRLLEERAPLYQAADLIVETSSLSVAAAAER
                     VIAFVTGRERGGPARDIRPAPAMVLGAPQQIRVDLGVRGYDVWIGGGLLADAAGHLGR
                     CGIRGRLALLTHARIEALYGRALVAGLRDAGYDVVTLTVPPSESSKSLRMAARLYDAL
                     LDARFDRSGALLALGGGVAGDLGGFVAATFLRGIAWVPVPTTLLAQVDASIGGKTGIN
                     LRAKNLVGAVHQPALVLSDIDTLRSLPLRELRSGMAEVVKTGVIGDPALFEVVEGRAR
                     ACLRRDPDLLAWIVGRCVVYKAGVVAGDEQEAGQRMVLNYGHTIGHGVEAAANYRGLT
                     HGEAIAVGMTLEARLAVRLGVCDAALLDRQSRVLASLGLPVRLASLGRGTLPAPGRIV
                     AAMAHDKKASGGRLRFVLPEALGRTVLRDDVPAALVEEVLADG"
     gene            complement(3514..4644)
                     /gene="aroC"
                     /locus_tag="VEW91_10035"
     CDS             complement(3514..4644)
                     /gene="aroC"
                     /locus_tag="VEW91_10035"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYM91959.1"
                     /translation="MLRLLTSGESHGRGLLTILEGMPAGLPLTEAEITAQLARRQRGY
                     GRGGRMQIEQDRVELYAGVMGGLTIGAPIGMHIGNKDWRQSEPPLTRPRPGHADLAGV
                     LKYHFDDVRRVLERSSARETVARVAAGAVCRALLGQFGITLASHVVELGGISIGRRPN
                     RHEEIPVIAEESPLRCVDPEAEARMIAAIDKAKAEGDTLGGVFEVVVTGLPVGLGSYV
                     HWDRRLDGRLAQAVMSINAIKGVEVGEGFQEARLPGSTAHDEIFYDPERGFYRRTNRA
                     GGTEGGMTTGDPLVVRAAMKPLSTLRSPLASVDIVTKERVEAAVVRSDVTAVPAAGVI
                     GEAMVAFVLADAMVEKFGGDSLDQMHAAYEAHQRWVRAGPGS"
     gene            complement(4662..5615)
                     /locus_tag="VEW91_10040"
     CDS             complement(4662..5615)
                     /locus_tag="VEW91_10040"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYM91960.1"
                     /translation="MGPAGSCLRVRRFPIIWDDPGRYAEGTTGIDGTTTVVGIIGDPV
                     RGSLSPRLHNAAFAALGLNWCYVAFQVSRDRLEEALRGLPALGIAGVNVTVPHKEAAL
                     AYLDETTDDARLIGAVNTIRVDGDRLIGHNTDTAGLLDALRYDGGVAVRGQRAAIVGA
                     GGAARAAAFALAGEGAAGVTIINRNWDRAAALADSIRRAFRCAVDAVPLDGSAVGAAL
                     REATLLVQATTVGGGAQRALSPIPGGALHPGLFVIDMVYSPEETVLLREARAAGCRAL
                     GGLPMLVYQGARAFEIWTGRAAPLQVMRDAAGLPEEAASKT"
     gene            5701..>6002
                     /locus_tag="VEW91_10045"
     CDS             5701..>6002
                     /locus_tag="VEW91_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91961.1"
                     /translation="MRYEGQEHYEIDVAGLRRKLPIVRISDSLWIAAFVLWGDVELTN
                     ACARAIAARLGHLEFEYVVSIEAKALPLVHMVATYLSDPMAGRYRPYVVLRKSVKG"
BASE COUNT          846 a         2294 c         2059 g          803 t
ORIGIN      
        1 cgtcgatcgc ccggggaatg agggcgcccc cgatcgccgc gccgcggagc cgggtcgcgc
       61 ggacgtccac ggtccccacc ggctccccgc cctcctcccg gacgtcccgc acgtccacgc
      121 gtgctcccat catcctgagg atatccagca cgccggtgcg ggtggggttg aggccgacgc
      181 cggcgaccgc cagctcggac ccgggcacga gcgcagcggc caccaggaaa aacgcggccg
      241 aggaaaggtc tcccggcacg cgcacctccg cgccccgcag cgtggcaggg ccgcgcaggc
      301 gggtggtcag cccgtcgcgg gccaccgcgc cgccgaacgc ccccagcatg cgctcggtgt
      361 ggtcgcggct ctgggccggt tcctcgaccg aggtctcgcc ttcggcgaac agcccggcga
      421 ggagcaccgc tgatttcacc tgggcgctcg cgaccggggt gcggtacggg atgccctgca
      481 gcccgccgcc ctcgatcgcg agcggggcca gccggcctcc ctcgcggccg gagatccgcg
      541 cccccatccg ccgcagcggg tccgcgacgc ggtccatcgg ccttcggcgg atggacgcgt
      601 cgccggtgag cacgctgtga aaccggtggc cggcgagcac cccggcgagg agccgcagcg
      661 tggtcccgga gttgccgaca tccaggacat cgcccggctc ctggagcggg cctccctgaa
      721 tctccaggcc gtccccctgc tcacggatcg cgacgccgag cgcccgcaga caccggaggg
      781 tcgacaggca atcctccgct ctgaggaatc cgaagacgcg cgtggggccc cgggcgatcg
      841 cgcccaggat cgccgcccga tgggagatgg acttgtcccc gggcacgcgg aggacgcccc
      901 ggagcgggcg ccccggggtg atgacgagcg gcgtcacgat cgggaggtca ggtcggggcg
      961 gagtcgctct gcaccgcgga ggtagatatg gcggacctcg gacgcggtct ccgtggtgtt
     1021 gacgaacatg aggatccgca cgcatcgctg aagggcgctt gggacgggga cctcctgggc
     1081 gcacatcatc gggacgtggg cccatcctcg aaggcgccgc ccggcctcgg cgggaaaggt
     1141 agcgttgagg tcgggggtca ccgtgaacag gatgccggcg atatcgtccg cgtcgacctc
     1201 gttggctttg accatttcct gcaggagctc ctgcgtggcg tccaggatcg ccttctccgt
     1261 gttggcgtcg accgtgaccg cgccccgaat cccgcggaac cgcattgttc cctcccgccc
     1321 ctcacggcga tggtggcctg tttgcttcga cggacgccta cgccttcctc cggggccgcg
     1381 gcgcgcggcc tgcgcgcggg gcgggggtgg ggcggggcgg ggcctccgcg agggcgagcg
     1441 cggcgcgcag gcccagcaga taactgtgca cgccgaaccc gatgatctgc cctttcgcga
     1501 cgggcgcgat caccgaggtg tgccggaatg cctcgcgggc gtggatgttg gagacgtgca
     1561 cctcgaccac cgggaccgcg atcgacgcga tcgcgtcgcg aagggcgtag ctgtaatggg
     1621 tcagcgcccc ggggttgagc acgatcgcgc cgtagcgacc ccgcgcggcg tgcagccggt
     1681 cgatgatggc cccttcgtgg ttgctctgaa acgtctcgac cgtggccccc gcctcgcgcg
     1741 ccagcgcctg gagcctccga tcgatctccg ccagcgtgat cgtgccgtag accgacggct
     1801 cccgttcccc gagcaggttc aggttcggcc cgtggatcac cagcacccta gccatcggcc
     1861 agcacctcct cgacgagcgc agcaggcaca tcgtctcgga gcaccgtgcg accgagcgcc
     1921 tccggcagca cgaagcgcag tcggcccccg ctcgccttct tgtcgtgggc catggccgcc
     1981 acgatccggc ccggcgcggg gagcgtcccg cgcccgaggg aagcgagccg caccggcagc
     2041 ccgagggagg ccagcacccg gctctgacgg tcgagcagcg cggcatcgca cacgccgagc
     2101 cgcacggcca gacgggcttc gagcgtcatc ccgacggcga tggcctctcc gtgggtgagc
     2161 ccgcggtagt tggccgccgc ctcgacgccg tggccgatcg tgtggccgta attgagcacc
     2221 atccgctgcc ccgcctcctg ctcgtcgccg gccaccacgc cggccttgta taccacgcac
     2281 cggccgacga tccaggcgag caggtcggga tcgcggcgga ggcaggcccg cgcgcgcccc
     2341 tcgacgacct cgaacagcgc cggatcgccg atcacgccgg tcttgaccac ctcggccatc
     2401 cccgaccgga gctcgcgcag cgggagcgac cgcagcgtgt cgatatccga aaggacgagc
     2461 gcgggctggt ggaccgcgcc gaccagattc ttcgcgcgca ggttgatccc cgtcttgccg
     2521 ccgatgctgg cgtcgacctg cgccagcagc gtggtgggca ccggcaccca ggcgatgccg
     2581 cgcaggaagg tggcggcgac aaacccgccc aggtctcccg ccacgccccc gccgagcgcg
     2641 agcagcgccc cgctccggtc gaatcgggca tcgagcagcg cgtcgtacag ccgcgcggcc
     2701 atgcgcaggc tcttgctcga ctccgacggc ggaacggtca gggtgacgac gtcgtacccc
     2761 gcgtcgcgaa ggcccgccac cagcgcacgg ccgtacagcg cctcgatgcg cgcatgggtg
     2821 agcagcgcga ggcggccccg gatcccgcac cggccgaggt gcccggcggc atccgccagc
     2881 agccccccgc cgatccagac gtcgtacccc cggaccccga gatccacgcg gatttgctgc
     2941 ggggctccga gcaccatcgc cggcgcgggg cggatgtcgc gcgcaggccc tccccgctcg
     3001 cgccccgtga caaacgcgat cacgcgctcg gccgcggcgg cgaccgaaag cgacgaggtc
     3061 tctacgatga ggtcggcggc ctggtacagc ggggcccgct cctcgagcag ccggcggacg
     3121 ctcccctcgg ggtcgtcccc cagcaacggc cgcgtgccct cgccccccac ccgcgcgagg
     3181 atggactgcg gatcggcatg gagcgcgacg atcaggccgt cccgccgcag gcggtccatg
     3241 ttctcacggc tgaggacaac gccgcccccg gtggcgatca cgctcccgcc gcgggcggtc
     3301 gcctccgcga ccgcctgcgc ctcgagccgc ctgaacgctg cctcgccgtc ctccgcgaag
     3361 atccgcggga tcggccgccc ggccaggcgc tcgatgatcg cgtcggtgtc caggaacggg
     3421 cgccccagcc gggatgcgac cagctcaccg accgcggtct tgccggtacc catgaagccg
     3481 atcaggacga tgttgggccg cacggggcgc ctcctagctc ccgggcccgg cacggaccca
     3541 ccgctggtgc gcctcgtagg ccgcgtgcat ctgatccagg ctgtcgcccc caaacttctc
     3601 caccatggca tcggccagca cgaacgccac catcgcctcg ccgatcaccc cggccgccgg
     3661 caccgcggtc acgtcgctgc gaacgaccgc cgcttcgact cgctccttgg tgacgatgtc
     3721 gaccgaggcg agcggcgagc gcagggtgct gagcggcttc atcgcggcgc ggaccaccag
     3781 cgggtcgccg gtggtcatgc ccccctcggt tccgcccgcc cggttcgtgc gccggtagaa
     3841 cccacgctcg ggatcgtaga agatctcgtc gtgcgcggtg gagcccggca gccgcgcctc
     3901 ctggaacccc tcgccgacct ccaccccttt gatcgcgttg atcgacatga ccgcctgggc
     3961 gagccggccg tccagccgcc ggtcccagtg gacgtacgat cccagcccga ccggcagtcc
     4021 ggtcacgacg acctcaaaca ccccgccgag ggtgtcgccc tcggctttgg ccttgtcgat
     4081 cgccgcgatc atccgggcct ccgcctcggg atcgacgcac cggagcgggg actcctcggc
     4141 gatcacaggg atctcttcgt gccggttggg gcggcgcccg atcgagatcc cgccgagctc
     4201 caccacgtgg ctcgcgagcg tgatgccgaa ctgtccgagc aacgcccggc agaccgcgcc
     4261 ggccgccacc cgtgcgaccg tctcacgcgc gctcgaccgc tcgagcaccc gacgcacatc
     4321 atcgaagtgg tacttgagga cgccggccag gtcggcgtgc ccggggcgcg ggcgggtgag
     4381 cgggggctcg ctctgccgcc agtccttgtt gccgatgtgc atcccgatcg gcgcgccgat
     4441 cgtgagcccg cccatcactc cggcgtagag ctcgacccgg tcctgctcga tctgcatccg
     4501 ccccccgcgg ccgtagccgc gctgccggcg ggcgagctgc gccgtgatct cggcctcggt
     4561 cagcgggagg ccggcgggca tgccctccag gatcgtcaac agcccgcgtc cgtgtgattc
     4621 tcctgaggtg agcaaccgaa gcaccgaaca ccaccgtccg actacgtctt ggacgccgcc
     4681 tcctcgggca accccgccgc gtcgcgcatc acctgcaggg gggcggcgcg gccggtccag
     4741 atctcgaaag cccgggcccc ctggtagacg agcatgggga ggcccccgag cgcgcgacag
     4801 cctgcggcgc gggcctcccg gagcagcacg gtctcttcgg ggctgtagac catgtcgatg
     4861 acgaacaggc cggggtgcag cgccccgccg gggatcggcg acagcgcgcg ttgcgcgccc
     4921 ccgcccaccg tcgtggcctg caccagcagg gtcgcctcgc gcagcgccgc ccccaccgcg
     4981 cttccatcga gcggcacagc gtcgaccgcg caccggaacg cccggcggat gctatccgca
     5041 agcgccgccg cgcggtccca attccggttg atgatcgtca ccccggcggc cccctccccg
     5101 gccagggcga atgccgccgc ccgggccgcg ccccccgcgc cgacgatcgc cgcgcgctgc
     5161 ccccgcaccg ccaccccgcc gtcgtatcgg agggcgtcca gcagcccggc cgtgtccgtg
     5221 ttgtggccga tcagccggtc gccgtccacg cggatcgtgt tgaccgcgcc gatcaggcgg
     5281 gcgtcgtccg tggtctcgtc gaggtaggcg agggcggctt ccttgtgcgg gacggtgacg
     5341 ttcacccccg cgatcccgag cgcgggcagc ccccgaagcg cctcctccag gcggtcacgg
     5401 gagacctgga acgcgacgta acaccagttc agccccaacg cggcgaacgc cgcgttatgg
     5461 agccgcggcg agagacttcc ccgaaccgga tccccgatga tgccgacgac cgttgtcgtc
     5521 ccgtcgatcc ccgtggttcc ctccgcgtat cttcccgggt catcccagat tatagggaag
     5581 cgcctgacac gaagacaaga gcccgccggc cccatgcgcc cggagggccc tggacagcgg
     5641 gcgtgcccgc gtgccagaat ggcaaacggt cgacgatccg atcctcacgg agggccaggg
     5701 atgcggtacg aggggcagga gcactacgag atcgacgtcg ccggactccg ccgcaaactc
     5761 cccatcgtgc ggatcagcga ttcgctgtgg atcgccgcgt tcgtgctctg gggggacgtc
     5821 gagctcacca acgcctgcgc ccgggcgatc gctgcgcggc tcgggcacct ggagttcgag
     5881 tacgtggtct cgatcgaagc caaggccctc cccctcgtcc acatggtcgc cacctacctc
     5941 tccgacccga tggccggccg ctaccgtccc tacgtcgtgc tgcggaagag cgtcaagggc
     6001 ta
//
LOCUS       DASQGH010000511         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55791, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000511 DASQGH010000000
VERSION     DASQGH010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55791"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1697
                     /locus_tag="VEW91_10050"
     CDS             285..1697
                     /locus_tag="VEW91_10050"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYM91962.1"
                     /translation="MEAVELCFMPATAMASAIRARKLSPVEVVDAVLARIDRLNPRLG
                     AYCTVTHAAARAAAKEAEVAVMRGDALGILHGVPVSVKDLIPTRGVRTTWGSRLFEHF
                     IPDEDAPVVDRLKQAGAIVLGKTNTPEFGYKGTTDNLVFGLSRNPWSLAHTPGGSSGG
                     GAAAVAAGLGPLAVGTDGGGSVRIPCSCCGIFGLKPTLGRVAAAPTYGGLDTLSHTGP
                     MTRTVGDAALMLNAIAGPDPRDLSSLPADGTDYLALLDRGIAGLRVAWTPDWGYAAVD
                     PEVRRITEAAAKRFADAGCAVEEDTPGFSDPADPFLVVYSASYAARFADKLPEARDRL
                     DPALVTLIEHGMRWTAAEFVTAANCRRTLHDVFRGFFARYDLLLTPTLAAPPLPVGVD
                     AYEEIAGRPVARTGWYAFTYPLNLTGYPAATVPCGWTAGGLPVGLQIVGPRFADAVVL
                     RAAAAFEALAPWAGKRPPVG"
     gene            complement(1704..>2196)
                     /locus_tag="VEW91_10055"
     CDS             complement(1704..>2196)
                     /locus_tag="VEW91_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91963.1"
                     /translation="LQEAGYDGKPFQFMVTKTYDYMYKSALVAKGQLEAVGFKVNLQV
                     VDWATLVQRRNNPDLYDVFTTGFVFSPADPTAQNVFLSSRWPGWWTSEKKEAVLQQFT
                     QTVAPDKRKAAWSQLQDIFYQEVPVVKLGDYYLLNVSQSWVKGFVNTPNLFLWNVWLS
                     RRS"
BASE COUNT          322 a          748 c          772 g          354 t
ORIGIN      
        1 cccgtcgtca taacggccac ctctcccggc cggcggtgag agcccggcca tccctgtatc
       61 gccatatgca ccggccagag ttccatccgg ggggcgctcg cccttggttc gtcctgggga
      121 ttttgtggat ggttcggatg aaggggcccc cccgttcaga tgagcgaacg gccggggctc
      181 ggtcgggggg caagttcccc ggaagatccg cagaagattt ccggcacagg agggggaaac
      241 ctagtacgat gaccggggac acgggcatct cggagggatg agcgatggaa gcggtggagc
      301 tgtgcttcat gccggcgacg gcgatggcgt cggcgatccg cgccaggaag ctttccccgg
      361 ttgaggtggt cgacgcggtc ctcgcgcgca tcgaccgcct caaccccagg ctcggcgcct
      421 actgcacggt cacgcacgcg gcggcccgcg ccgcggccaa ggaggccgaa gtcgccgtga
      481 tgcggggcga tgcgctgggc atcctccacg gtgtccccgt gtcggtcaag gacctgatcc
      541 cgaccagggg cgtgcggacc acgtgggggt ccagactctt cgagcacttc atccccgatg
      601 aagacgcccc ggtggtcgac cggttgaagc aggccggggc catcgtcctc gggaagacca
      661 acaccccgga gtttggctac aaggggacca ccgacaacct ggtcttcggg ctgagccgca
      721 acccgtggag cctggcccac acccccggcg gatcgagcgg cggcggcgcc gccgccgtgg
      781 cggcgggact cgggccgctc gcggtcggga cggatggggg aggctcggtc cggatcccct
      841 gcagctgctg cgggatcttc gggttgaagc cgaccctcgg gcgggtcgcg gccgctccca
      901 cctacggcgg gctggacacc ctttcccaca ccggccccat gacgcgcacc gtgggggacg
      961 cggccctgat gctcaacgcg atcgcgggcc cggacccccg cgacctgagc tccctgcccg
     1021 ccgacgggac cgactatctg gccctcctcg accggggcat cgcggggctg cgggtcgcct
     1081 ggaccccgga ctggggatac gccgcggtgg accccgaggt ccggcggatc acggaagcgg
     1141 cggcgaagcg gttcgcggac gccggctgcg cggttgaaga ggacacgccc ggattctccg
     1201 acccggccga cccgttcctg gtcgtctaca gcgcctcgta tgccgcacgg tttgccgaca
     1261 agctgcccga ggcgcgggat cgcctcgatc ccgcgctggt gaccctgatc gagcacggga
     1321 tgcgctggac cgcggccgag ttcgtcaccg cggccaactg ccggcgaacg ctccacgacg
     1381 tgttccgcgg gttcttcgcg cgctacgatc tgctcctgac cccgaccctg gcggcgcccc
     1441 cgctgcccgt gggggtcgac gcctacgagg agatcgccgg acgaccggtg gcgcgcacgg
     1501 ggtggtacgc tttcacctac cccctcaacc tgaccgggta tccggcggcc acggtgccct
     1561 gcgggtggac ggccgggggc cttccggtgg ggctgcagat cgtgggcccc cggtttgcgg
     1621 atgcggtggt gctgcgcgcc gcggcggcgt ttgaagccct cgccccctgg gcagggaagc
     1681 gtccgccggt cggctgagcc gttttacgag cgccgggaca gccagacgtt ccagaggaag
     1741 aggttgggcg tgttcacaaa gcccttcacc cacgactgcg acacgttgag caggtagtag
     1801 tccccgagct tgaccacggg gacttcctgg tagaagatgt cttgcagttg cgaccacgcc
     1861 gctttccgct tgtccggggc cacggtctgc gtaaactgct ggagcacggc ctctttcttc
     1921 tcgctcgtcc accaccccgg ccatctggag gagaggaaga cgttctgcgc ggtcggatcg
     1981 gccggcgaga agacgaaccc ggtcgtgaag acgtcgtaca ggtcggggtt gtttcgccgc
     2041 tgcacgaggg tcgcccagtc gaccacctgc aggttgacct tgaagccgac cgcctcgagc
     2101 tgccccttcg ccacgagcgc gcttttgtac atgtaatcgt aggtcttcgt gaccataaac
     2161 tggaacggtt tgccgtcgta cccggcttcc tggagg
//
LOCUS       DASQGH010000512         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33421, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000512 DASQGH010000000
VERSION     DASQGH010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33421"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VEW91_10060"
     CDS             complement(<1..305)
                     /locus_tag="VEW91_10060"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYM91964.1"
                     /translation="MMASAPSRDPRGTVGTDLAPALARPTRWAWAEIDLGAVRDNVRA
                     LVALLRPPTRLLAVVKADAYGHGAPVVARAAVEAGAWALGVATTDEGVQLRRAGIESP
                     "
     gene            complement(280..1854)
                     /locus_tag="VEW91_10065"
     CDS             complement(280..1854)
                     /locus_tag="VEW91_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HYM91965.1"
                     /translation="MKLPTPSEMAALDRRCQEAHGISVHALMEQAGQRTAEVARQLLR
                     SRGGRRVTVLVGKGNNGGDGLVAARHLAGDGPTRVLLVVPPQEIRGLPAEHLTALRDR
                     QVGIEEAQALPASRLREALRDADLIVDAIFGTGFRGPADGVPAQVIEAANASGVPILA
                     VDVPSGADAAMGRADPPCIHAVATVAMGLPKVGTVQFPAAAHVGRLFVADIGIPEAVV
                     REAAIPTELATAAWVDRTLPRRPADSHKGRFGRVVILGGSRGYAGAPVLAARGAIRAG
                     AGLVTIGLPSSLALVPPASLPEAMTRPLAENASGTLTEDGAQAVAELAGIADAVAVGP
                     GLSTHPDVVRLLYRVLPRFTCPIILDADGLNAFAGTPRRLRDVPGPLVITPHPGEMAR
                     LLGRDVQDVQGDRLGTAREAARLTGGVVILKGARTVVAGPEGRALVIPTGNAAMATGG
                     MGDVLTGAVAAFAGAGVPPFDAAGCAAYLHGLAGDLAAGSGELGLLAREVADHLPRAL
                     ARVRSGEVDDGISAVP"
     gene            complement(1874..2245)
                     /gene="acpS"
                     /locus_tag="VEW91_10070"
     CDS             complement(1874..2245)
                     /gene="acpS"
                     /locus_tag="VEW91_10070"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HYM91966.1"
                     /translation="MIRGLGIDVIEVDRIRRAVARWGEGFLFRVFTPAELSRGSASPG
                     AAERLAGRFAAKEAVMKALGLGRLGVGWQEIEITVDPLGKPGVRLMGRAAAVADRLGI
                     RAWRLAISHTRLLAIAQALAE"
     gene            complement(2258..2422)
                     /locus_tag="VEW91_10075"
     CDS             complement(2258..2422)
                     /locus_tag="VEW91_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HYM91967.1"
                     /translation="MIDKELLEILACPACKAPVRQEGDRIVCTKCGLRYPIRDDIPVM
                     LVDEAERPAG"
     gene            complement(2412..3350)
                     /locus_tag="VEW91_10080"
     CDS             complement(2412..3350)
                     /locus_tag="VEW91_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYM91968.1"
                     /translation="MRILVTGGAGFIGSLVADAYLGAGHHVAVVDALEGNGQNRAPRS
                     ATFYREDIRRPALAEVFRQERPDVVSHHAAQVNLRRSLEDPVGDAETNVLGTVRVLDL
                     AVRFGAKQVIFASTGGAVYGEPRALPVGEDHPILPTAPYGLHKYLGEQSLDYYRRVHG
                     LGTVVLRYSNVYGPRQDATTEAGVVAIFVDALLSGRSPVIFGDGTQTRDFVYVEDVAE
                     ANLRVLGRTIEEPMHIASGTETSVNDLCARLRALTRTTVEAVHGPPVPGEVHRICLRV
                     GRAEALLGWRPRVRLEEGLRRTVEWFMAERSTVRDR"
     gene            complement(3347..5176)
                     /gene="glmS"
                     /locus_tag="VEW91_10085"
     CDS             complement(3347..5176)
                     /gene="glmS"
                     /locus_tag="VEW91_10085"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HYM91969.1"
                     /translation="MCGIMGYIGDRPAVPVLLDGLRRLEYRGYDSAGVAVVQNGGIEV
                     VKSAGKLAQLVRVVERRPLSGVVGIGHTRWATHGHPSDENAHPHADCRGRVVVIHNGI
                     IENFLALKEGLIARGHRFRSETDTEVLAHLIEEAYRGDLPRAVEEAVAQASGAYALVV
                     LSADEPDKIVAVRRISPLIVGLGQGETLLASDIPALLPYTRDVVLIEDGELAVLRRGG
                     VVLSRIGGGRIDRAPMRITWDTAAAEKGGYAHFMLKEIHEQPRALQETLMGRLPDDRT
                     IALDGVDLGGEFLRRLSKVWLVACGTAYYAGVVGHTLLERLAQVPAEPDLGSEFRYRS
                     PIVGAGTLAIAISQSGETADTLAAAREARARGARLLAVANVVGSTLAREADDVLYTRA
                     GPEIAVCSTKAFITQIAVLTMLATHLGQVRGTLTGERVAGLIQGLRALPARAQAVLDL
                     EPAIVTLAEHLRAAEDAFFIGRGLDYPVAMEGSLKLKEISYVHSEALAAGELKHGTLA
                     LVTPGVSVIAIGTQPEIIEKTASNIEEVRARGAEVIGIATEVTAEALGRHVDRLLVVP
                     AVDPLLAPALAVIPLQLLAYHVARLRGHDVDQPRNLAKSVTVE"
BASE COUNT          770 a         2065 c         1862 g          730 t
ORIGIN      
        1 ggcgattcga ttccggcgcg gcgcaactgc acgccttcgt cggtggtcgc cacgccgagc
       61 gcccacgctc ccgcctcgac cgctgcccgg gcgaccacgg gggccccgtg cccgtacgca
      121 tccgccttca ccaccgccag caggcgcgtc ggagggcgga gcagagcgac gagcgcacgc
      181 acattgtccc gcacggctcc gaggtcgatc tcggcccacg cccaccgggt cggcctggcg
      241 agggcggggg ccagatcggt ccccaccgtg ccgcgcggat cacgggacgg cgctgatgcc
      301 atcatccacc tctccagacc ggacgcgcgc gagcgcgcgc ggaaggtgat ccgcgacctc
      361 acgggcaagg agccccaact ctccggatcc ggcggcgagg tcgcccgcca gcccgtggag
      421 gtaggccgca caccccgcag cgtcaaacgg gggcaccccg gcccccgcga acgccgccac
      481 cgctccggtg aggacatcgc ccatcccgcc ggtggccatc gcggcgttcc cggtggggat
      541 gacgagcgcg cgcccctcgg gcccggccac gaccgtccgg gctcctttca gaatgacgac
      601 gccgcccgtg aggcgcgccg cctcccgggc ggtcccgagg cggtcgccct gcacgtcttg
      661 cacgtcccgc ccgaggaggc gggccatctc gccggggtgc ggggtgatca cgagcggccc
      721 cgggacatcg cgaagccggc gcggggtgcc cgcaaacgcg ttcaacccgt cggcgtccag
      781 gatgatcggg caggtgaacc gcggcagcac gcggtagagg agcctgacca cgtccggatg
      841 ggtggagagc ccgggcccca cggccaccgc atccgcgatg ccggcgagct cggccacggc
      901 ctgggcgccg tcctccgtga gcgtccccga ggcgttctcg gcgagcggcc gggtcatcgc
      961 ctccgggagc gaggctgggg gaacgagggc gagcgaggac ggcaacccaa tcgtcacgag
     1021 ccccgctccc gctcgaatcg cgcctcgggc cgccagcacc ggcgcccccg cgtaccctcg
     1081 gctgccgccc aggatgacca cccgcccgaa gcggcccttg tggctgtcgg cgggacggcg
     1141 cgggagcgtc cgatcgaccc aggccgcggt ggccagctcc gtcgggatcg cggcctcgcg
     1201 caccaccgcc tcggggatgc cgatgtccgc gacgaagagg cggccgacgt gcgccgccgc
     1261 ggggaactgc accgtgccga ccttcgggag gcccatggcg accgtcgcca ccgcatggat
     1321 gcacggcggg tcggcgcgcc ccatcgcagc gtccgctccg gagggcacgt ccaccgcgag
     1381 gatcgggacc cccgaggcgt tcgcggcctc gatgacctgg gcggggacgc catccgcggg
     1441 gcctcggaag cccgtgccga agatcgcatc cacgatcaga tcagcgtccc ggagcgcctc
     1501 tcggaggcgt gaagccggga gcgcctgggc ctcctcgatc cccacctgac ggtcccggag
     1561 cgccgtgagg tgttcagccg gcagtccgcg gatctcctgg ggcggaacga ccagcagcac
     1621 gcgcgtcggg ccgtctcccg ccagatggcg ggcggccacc aggccgtcgc cgccgttgtt
     1681 ccccttgccg accagcacgg tgacccgccg ccccccgcgc gatcggagca actggcgcgc
     1741 gacctccgcc gtccgctggc cggcctgctc catcagggcg tgcacggaaa tgccgtgggc
     1801 ctcctgacag cggcggtcca gcgcagccat ctcggagggg gtgggaagct tcaccggtcc
     1861 gccggacgcc ggctcactcg gcgagggcct gggcgatcgc gagcaggcgc gtgtgcgaga
     1921 tcgcgaggcg ccaggcccgg atgcccaggc gatcggccac cgcggccgcc cggcccatga
     1981 ggcgcacccc cggcttcccc agggggtcga cggtgatctc gatctcctgc cacccgaccc
     2041 ccaaacggcc gagcccgagg gccttcatca ccgcctcctt ggccgcgaac cgtcctgcga
     2101 ggcgctcggc cgcgccgggg gaggcgctcc cgcggctcaa ctctgccggc gtgaagacgc
     2161 ggaagaggaa tccctcgccc caccgggcca ctgcccgccg gatgcggtcg acctcgataa
     2221 catcgatgcc gagtcctcgg atcatccgct gcgcatccta gccggccggg cgttccgcct
     2281 catcgaccag catcaccgga atatcgtccc ggatcgggta ccggaggccg cacttggtgc
     2341 agacgatccg gtccccttcc tgccgcacgg gcgccttgca ggccgggcag gcgaggatct
     2401 cgagcagctc cttatcgatc acggacggta ctcctctccg ccatgaacca ttcgacggtg
     2461 cgccgaagcc cttcctccag cctgacccgg ggccgccatc ccagcagcgc ctccgcgcgg
     2521 ccgaccctga gacagatccg gtggacctcc cccgggaccg gagggccgtg gacggcctcc
     2581 accgtcgtcc gggtcagcgc gcgcagccgg gcacacaggt cgttgaccga ggtctctgtc
     2641 ccactggcga tgtgcatcgg ctcctcgatc gtccgcccca gcacgcggag gttcgcctcg
     2701 gccacatcct ccacatagac aaagtcccgg gtctgggtcc cgtccccgaa gatcaccggg
     2761 gaccgtccgc tcaggagcgc gtccacaaag atcgcgacca cgccggcttc ggtcgtggca
     2821 tcctgccggg gaccgtacac gtttgaatac cgcaagacga cggtcccgag accatggacg
     2881 cgtctgtagt agtcgaggga ctgctcgccg aggtacttgt ggagtccgta cggcgccgtc
     2941 gggaggatcg gatgatcctc gccgaccggg agggcgcgcg gctccccgta cacggccccg
     3001 ccggtcgagg caaagatcac ctgcttggcc ccgaaccgga cggccagatc gagcacgcgg
     3061 accgtcccga ggacgttggt ctccgcgtcg cccaccggat cctcgagcga ccggcggagg
     3121 ttgacctgcg cggcgtgatg cgagacgaca tccgggcgct cctgccggaa gacctccgcg
     3181 agggccggcc ggcggatatc ttcccggtag aacgtcgcgc tccgcggcgc ccgattctgt
     3241 ccgttcccct ccagggcatc gaccaccgcc acatggtgcc cggcgccgag gtaggcgtcg
     3301 gccacgaggg acccgataaa cccggccccg cccgtgacga ggatcctcac tccacggtca
     3361 cgcttttcgc caggttgcgg ggctggtcga cgtcgtggcc ccggaggcgc gccacgtggt
     3421 aggcgagcag ctgcagcggg atcaccgcca gcgcgggcgc gagcagcggg tcaacggccg
     3481 gaaccacgag gagccggtcc acgtgccggc ccaacgcctc ggccgtgacc tcggtcgcga
     3541 tcccgatgac ctccgccccc cgcgcgcgca cctcctcgat gttgctggcg gtcttctcga
     3601 tgatctcggg ctgcgtcccg atcgcgatca cggagacccc gggggtcacg agcgccagcg
     3661 tgccgtgctt gagttcgccc gcggcgagcg cctccgagtg cacgtagctg atctccttca
     3721 gcttgagcga cccctccatc gccaccgggt agtcgagccc gcgtccgatg aagaacgcgt
     3781 cttcggcggc gcgcaggtgc tcggccagcg tgacgatcgc gggttcgagg tccagcaccg
     3841 cctgcgcgcg cgcggggagc gcccgcagcc cctggatcaa ccccgcgacc cgctccccgg
     3901 tgagggtccc cctgacctgc cccaggtggg tggccagcat cgtgaggacg gcaatctggg
     3961 tgatgaaggc tttggtcgag cagaccgcga tctcggggcc ggcgcgcgta tacaggacat
     4021 cgtcggcctc gcgggccagc gtgctcccca cgacgttggc cacggccagg agcctggcgc
     4081 cccgggcccg cgcttcccgt gccgccgcca gcgtatcggc ggtctccccc gactggctga
     4141 tcgcgatggc cagggtcccc gctcccacga tcggcgatcg gtagcggaac tcgctcccca
     4201 ggtcgggctc ggccgggacc tgcgccagcc gctcgagcag ggtgtgcccg accacgcccg
     4261 cgtagtatgc cgtcccgcag gcgaccagcc acaccttgga cagccggcgc agaaactccc
     4321 ccccgaggtc cacgccgtcg agggcgatcg tccggtcgtc cggcagtcgc cccatgagcg
     4381 tctcctgcag ggcccgcggc tgctcgtgga tctctttgag catgaagtgc gcatacccgc
     4441 ccttctcggc ggcggcggta tcccaggtga tccgcatcgg tgcgcggtcg atccggccgc
     4501 ccccgatgcg ggacaggacg accccgcccc gccgcagcac cgcgagctcg ccgtcctcga
     4561 tgagcacgac gtcgcgcgtg tagggcagga gggccggaat gtcggacgcg aggagcgtct
     4621 ccccctgccc caatccgacg atcagcgggc tgatccgccg gacggcgacg atcttgtccg
     4681 gctcgtccgc cgacagcacg accagcgcgt acgcccccga cgcctgcgcg accgcctcct
     4741 cgaccgcgcg ggggagatcc ccgcggtagg cctcctcgat gaggtgcgcc aggacttcgg
     4801 tgtcggtctc ggaccggaac cgatggccgc gcgcgatgag cccttccttg agcgcgagga
     4861 agttctcgat gatgccattg tggatgacca cgacccggcc ccggcagtcg gcgtgcggat
     4921 gcgcgttctc atccgacggg tgcccgtggg tcgcccaccg ggtgtgcccg atccccacca
     4981 ccccgctgag gggcctccgc tccaccaccc gcaccaactg agcgagcttg ccggcggatt
     5041 tcacgacctc gatgccgccg ttctggacaa cggccacacc ggccgagtcg tagccccggt
     5101 attccagacg gcgcagaccg tccaggagta ccgggaccgc gggccggtcc ccgatatacc
     5161 ccatgatgcc gcacatggat gcctcccgat cgccccgcgc agggtcgtac cggcactaag
     5221 cggccttggg atagtctgtg ggggcgggac tccggcagcg gcaggcgtgc ctgctcccag
     5281 cggcgcgcag gtggggtgac cgccgcaccg ctcgctacct tggattgtgc tcgtgtcccg
     5341 gacgcctttt gtccccgggg tcgcccccgg gttttggtag cgtccttgag gccggaccgg
     5401 cctagggtca cccgccgaaa aggagcc
//
LOCUS       DASQGH010000513         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8018, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000513 DASQGH010000000
VERSION     DASQGH010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8018"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..>1509
                     /locus_tag="VEW91_10090"
     CDS             371..>1509
                     /locus_tag="VEW91_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91970.1"
                     /translation="MTNRIAALGVLLLLVLPVQGATAGPAPGTFVTVVGDNPDSLNPY
                     LHSLAASSAVFRYTFDSLYTVNSKGEWIPALAVGYTVSPDALAWTFKLRPGVSWSDGA
                     PFTAADVKFSWQLVTNKDVRITYATGFDKIASIDTPDPLTVVYHLKEPYAPFRDQVMG
                     AAVVPQHVLGALTGDQVNRAPFNQKPVGTGPFTISEFVTDDHVTLTANPQYWGKKAKL
                     RRIIVRIVPDQNAQVNLLRAGDLSVLTVPAARLEEVKRMPNIVIKRYLEATYALVQLD
                     EYEFLREVPVRQALDFATPKGSIIRNIMKGQAEPAVSDMVPNGPWAHKALHPRPYDLA
                     QARRILRESGFTPGPGGFLYRGGRRLEIPLWTLSGRVNFVQAVQV"
BASE COUNT          262 a          520 c          481 g          246 t
ORIGIN      
        1 ccttggaccg ctcggccacc cctccggcgt tcagtgtacc cagcaaaccc tcaattgaca
       61 agcaagaatt cgcccgtgca tgctgcttac ggcggcatcc gcggaattcc tagcaccgcg
      121 cgaaagacac gcaggaaatt ctcgcccaag atcttccgga catccgcgtc tgacagaccc
      181 cgccgcagca tcgcctcggt cagtgtggga agatcctggg gacccgtcag gcccgggatc
      241 gtggtcttcg cttcctcccg cgcaggccga tcccgtgtgt cgacaggttg cggccctgcc
      301 cgggtctgcc tagaatgagg aaaggggcat tcatggaccg aaggcgcccg ctacaccgac
      361 gggagggtct gtgacgaacc ggatcgccgc gctgggggtg ctacttctcc tggtccttcc
      421 ggttcagggc gcgaccgccg gcccggcccc gggaaccttc gtgaccgtgg tgggcgacaa
      481 ccccgacagc ctgaatccct acctgcacag cctggcggcg agcagcgccg tctttcggta
      541 cacgtttgac agcctctaca cggtcaacag caagggggag tggattcccg cgctggccgt
      601 cggctacacg gtcagcccgg acgccctcgc ctggaccttc aagctgcggc ccggggtgtc
      661 ctggtcggac ggcgcgccgt tcaccgcagc ggatgtgaag ttctcctggc agctcgtgac
      721 aaacaaagat gtccgcatca cctacgccac ggggttcgac aagattgcct cgatcgacac
      781 cccggacccc ctgactgtcg tgtaccattt gaaggagccg tacgcgccgt tccgcgacca
      841 ggtgatgggg gcggcggtcg ttccccagca cgtgctggga gcgctcaccg gggaccaggt
      901 caaccgcgcg ccgttcaacc agaagccggt cggcaccggg ccgttcacca tctccgagtt
      961 cgtcaccgac gatcacgtga cgttgaccgc caacccgcag tactggggga agaaggcgaa
     1021 gctgcggcgg atcattgtgc gtatcgtccc cgaccagaac gcgcaggtga acctgctcag
     1081 ggcgggggat ctcagcgtcc tcaccgtccc ggcggcgcgc ctcgaggagg tgaagcgcat
     1141 gcccaatatc gtgatcaagc gctacctcga ggccacctac gccctggtgc agctcgacga
     1201 gtacgagttc ctccgcgagg ttcccgtccg gcaggcgctc gacttcgcga cccccaaggg
     1261 gtcgatcatc cggaatatca tgaagggcca ggcggagccg gcggtgagcg acatggtgcc
     1321 caacggcccg tgggcccaca aggccctcca cccccgcccg tacgacctcg cgcaggcgcg
     1381 caggatcctg cgcgagagcg gcttcacccc ggggcccggc gggttcctgt acaggggcgg
     1441 caggcgtctc gaaattcccc tgtggacgct ctcgggccgg gtgaacttcg tgcaagcggt
     1501 gcaggtcat
//
LOCUS       DASQGH010000514         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41832, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000514 DASQGH010000000
VERSION     DASQGH010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41832"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VEW91_10095"
     CDS             <1..968
                     /locus_tag="VEW91_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYM91971.1"
                     /translation="ALLPAVEDGTVTLIGATTQNPFFEVTPTLVSRSRVFTLEPLGSD
                     DLITILRRAVADPRGLGPEAVDLSDDAARYIAGLANGDARVALNILELAAGTTPPDAD
                     AVRRITLEVAGEAGQRRALLYDRDGDAHYDTISAFIKSIRGSDPDAAVYWLARMLAAG
                     EDPKFIARRMVVHAAEDIGLADPQALLVAVAAAQAVEFVGLPEARIPMAEAAIYLATA
                     PKSNATITAISRAWHDVEHTEAHPVPRHLRDASYPGAHRLERGSGYQYPHDYPGGFVA
                     QQYVPENIKDRTYYEPTTAGYEGEVRRRLQAWWEGVKRYAFLGRA"
     gene            974..2122
                     /gene="mnmA"
                     /locus_tag="VEW91_10100"
     CDS             974..2122
                     /gene="mnmA"
                     /locus_tag="VEW91_10100"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYM91972.1"
                     /translation="MGRVAVAMSGGVDSSVAAALLVADGHEVVGFTMNLWPTWVSQEG
                     EGQGCCGIGAIDDARSVARVLGIRHYLLNLRAEFERDVIGHFASEYARGRTPNPCIAC
                     NRAIKFDLLWRRVRGLGMELLATGHYARVDRSADGRFRLLRAADRRKDQSYVLAALTQ
                     DQLAHLRFPVGAYTKPQIREIARRHGLCVADKPDSQEICFVPGGDYAAVVERFAPHAL
                     RPGPIYDLGGRRLGEHHGLARYTVGQRRGLGLGGGGAWYVVQIDPGRNALTVGDAASV
                     QCPELVAGEVNWIAIPRLEGPRRVGVRIRHASVDVPALIAPAPEGRVAVRFETWPRAA
                     APGQAAAFYDGEIVLGGGVIEEVRMHAPVPDSGLAVASAGPESMGRVC"
     gene            2150..2551
                     /locus_tag="VEW91_10105"
     CDS             2150..2551
                     /locus_tag="VEW91_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91973.1"
                     /translation="MSTIADVSLAIIAATCIISVVAFIVLVVLVWRVTSRVQAMLALI
                     HRALPELVTDTRAILAKVDREIVGEVARAVTHVSAAVGSGVSAFEQVQTTARRVAEDI
                     ILPQMATAAGLWAAVREGLAWLRPGGDGKRR"
     gene            2602..3000
                     /locus_tag="VEW91_10110"
     CDS             2602..3000
                     /locus_tag="VEW91_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HYM91974.1"
                     /translation="MGTDEPRDFLSGLIVGSLVGIALGLLFAPESGERTRERIRKQGK
                     EWGKEARERADRAAERVRTTAGDVAERVREHADEFATRARSTAEDVVQRGRAAVEEKA
                     DRLRRAYEQGRDSSGGWSHDRPRKPEGPEG"
     gene            3003..3431
                     /locus_tag="VEW91_10115"
     CDS             3003..3431
                     /locus_tag="VEW91_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM91975.1"
                     /translation="MPGRRGTGHAAETVEVTIPPKPEFLSVARLTAATVAARQLFTYD
                     EIEDLKIAVSEACTALILAGTAGHPLTLRFAMDADALVVRILTEGPGVALEAARLHAR
                     KPLDETRLGVFLMQCLVDEVEVRRGEEGKAELRLVKRRQR"
     gene            3431..4219
                     /locus_tag="VEW91_10120"
     CDS             3431..4219
                     /locus_tag="VEW91_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HYM91976.1"
                     /translation="MALRRRRRRPLSRTELRALFGRLRQANDPAAREALILAYHSLAA
                     YLAGKFVDRGEPLEDLVQVAQIGLINAVDRYDPTRGIEFTTFATPTIVGEIRRHFRDK
                     LPSIRIPRRLREVHHTLMRSVESLSQRVGRSPTIPELAEATGIPFDVVLEALEAGRAY
                     VPVSLDAGGPEEDGDPATLLASLGREDEALQGLEDQATLAWAIGRLPERERELVSLRF
                     FKRLSQGQVARRLGISQMHVSRLQRAALARLRALIVRGPAHPHS"
     gene            4231..>4780
                     /locus_tag="VEW91_10125"
     CDS             4231..>4780
                     /locus_tag="VEW91_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HYM91977.1"
                     /translation="MPDLATAAEIAERLRAAGFETYLVGGCVRDMLLGREPADYDIAT
                     AAKPEEIRALFPRTIDVGAAFGVIRVLAPNGEYEVATFRTEGPYLDGRHPASVQYATV
                     REDVARRDFTVNGLLYDPKAAEVLDFVGGRADLAARRIRTIGEPAARFAEDRLRMLRA
                     VRLAAELDFEIAPEVTAAARQLA"
BASE COUNT          696 a         1641 c         1759 g          684 t
ORIGIN      
        1 acgccctgct gccggccgta gaggacggga ccgtgaccct gatcggggcg accacccaga
       61 acccgttctt cgaggtcacg cccacgctcg tgtcgcggtc gcgggtgttc accttggagc
      121 cgctcggcag cgacgacctc atcacgatcc tccggcgcgc ggtcgcggat ccccgggggc
      181 tcggcccaga ggcggtggac ctcagcgacg atgccgcccg gtacatcgcc ggcctggcga
      241 acggggatgc gagggtggcg ctcaacatcc tcgagctggc ggccgggacg accccgccgg
      301 atgccgatgc ggtacgccgc atcacgctcg aggtcgccgg ggaggcggga cagcgccggg
      361 cgctcctcta cgaccgggac ggcgacgcgc actacgacac catctcggcc ttcatcaaga
      421 gcatcagggg atcggacccc gacgcggcgg tctactggct cgcgcggatg ctggccgcgg
      481 gggaggaccc gaagttcatc gcgcgccgca tggtggtgca cgcggccgag gacatcgggc
      541 tggcagatcc gcaggcgctg ctggtggcgg tcgccgcggc ccaggcggtg gaattcgtcg
      601 ggctgcccga agcgcgcatc ccgatggccg aggccgcgat ctacctcgcc accgcgccga
      661 agagtaacgc gacgatcacc gcgatctcgc gggcgtggca cgacgtggag cacacggagg
      721 cccacccggt cccccgccat ctgcgggacg cctcataccc gggagcccac cggctcgagc
      781 gggggagcgg gtatcagtat ccccatgatt acccgggagg gttcgtcgcg cagcagtacg
      841 tgccggagaa catcaaggac cgcacatact acgagccgac gaccgcgggg tacgaagggg
      901 aagtgcgccg ccgcctccag gcgtggtggg aaggcgtgaa gcggtacgcg tttctcggga
      961 gggcttgatc ggcatgggcc gcgtcgcggt ggccatgagt ggcggggtcg acagttcggt
     1021 cgccgccgcc ctgctcgtgg ccgacgggca cgaggtcgtc ggattcacca tgaacctctg
     1081 gccgacctgg gtgtcgcagg agggggaggg gcaggggtgc tgcggcatcg gggcgatcga
     1141 cgacgcgcgg tcggtcgccc gggttctggg gatccgccac tacctgctca acctcagggc
     1201 ggagttcgag cgggatgtca tcgggcactt tgccagcgag tacgcccgcg ggcgcacccc
     1261 gaacccgtgc atcgcctgca accgggcgat caagttcgac ctcctctggc gccgggtccg
     1321 cgggctcggc atggagctcc tggccaccgg ccactacgcc cgcgtggatc ggagcgcgga
     1381 cggccggttc cggctcctgc gggcggccga ccggcgcaaa gaccagtcgt acgtgctggc
     1441 cgcgctcacc caggaccaac tcgcccacct gcgctttccc gtcggcgcct acaccaagcc
     1501 gcagatccgg gagatcgccc gccgccacgg cttgtgcgtc gccgacaagc ccgacagcca
     1561 ggagatctgc ttcgtccctg gcggcgatta cgccgcggtg gtcgagcggt ttgcgcccca
     1621 cgccctgcgc cccggcccga tctacgatct gggcggccgc cgcctaggag agcaccacgg
     1681 gctcgcccgg tacacggtgg ggcagcggcg ggggctcggg ctcggcggtg ggggtgcctg
     1741 gtacgtcgtc cagatcgatc cgggacgcaa cgccttgacc gtgggggacg cggcatccgt
     1801 gcagtgcccc gaactggtgg cgggggaggt caactggatc gcgatcccgc ggctcgaggg
     1861 accccggcgg gtgggggtca ggatccgcca cgcctcggtg gatgtccccg ccctgatcgc
     1921 cccggccccg gaaggacggg tcgccgtccg gttcgagacc tggccccgag ccgccgcccc
     1981 ggggcaggcg gccgcgttct acgatggcga aatagtgctc ggcggaggcg tgatcgagga
     2041 agtcaggatg cacgcccccg tgccggattc gggattggcc gtggcgtctg ccgggccaga
     2101 gtctatgggc cgggtgtgtt agggtattct ttaaggtagg aggggagccg tgtcgaccat
     2161 cgccgacgtg agcctggcga tcatcgcagc cacctgcatc atctcggtcg tggcgttcat
     2221 cgtcttggtc gtgctcgttt ggcgggtcac atcccgcgtc caggcgatgc tggcgctgat
     2281 ccatcgggcg cttcctgagc tcgtcaccga tacccgggcc atcctcgcca aggtcgatcg
     2341 cgagatcgtg ggggaggtcg cccgggcggt cacccatgtc agcgccgccg tgggatccgg
     2401 ggtcagcgcg ttcgagcagg tccagaccac cgcccgacgg gttgccgagg atatcattct
     2461 cccccagatg gcgacggcgg ccggtctctg ggcggcggtc cgggagggac tcgcctggct
     2521 ccggcccggc ggagacggaa agcggcgcta ggccgtttcg aaaacgcgca gcacgggcag
     2581 gcaaaacagg gagggatgag gatggggacg gacgagccgc gagatttctt gtcgggattg
     2641 atcgtgggca gcctggtggg gatcgccctc ggcctgttgt ttgctcccga gtcgggggag
     2701 cggacccggg aacggatccg caagcagggg aaggaatggg gcaaggaggc ccgcgagcgc
     2761 gcggatcggg ccgccgaacg ggtccgcacg accgccggcg acgtcgcgga gcgcgtgcgc
     2821 gagcacgccg atgagttcgc cacccgtgcg cggagcaccg ccgaggacgt cgtgcagcgc
     2881 ggccgtgcgg ccgtcgagga gaaggccgac cgcctccggc gggcctacga gcaaggacgc
     2941 gactcatcgg gcggctggtc gcacgaccgg cccaggaagc cggagggccc ggagggctag
     3001 gggtgccggg gaggcggggg accgggcacg cggcggagac cgtcgaggtc acgatccctc
     3061 ccaagccaga gttcctcagt gtcgcccggc tcaccgcggc gaccgtggcg gcgcggcaat
     3121 tgttcaccta cgatgagatc gaggatctca agatcgcggt gtccgaggcc tgcaccgcgc
     3181 tcatcctcgc cggcaccgcc ggccatccgc tcacccttcg gttcgccatg gatgcggacg
     3241 cgctcgtggt ccgcatcctc accgaggggc cgggggtcgc gctcgaggcc gccaggctcc
     3301 acgcaaggaa accgctggac gagacccggt tgggcgtgtt cctgatgcag tgcctcgtcg
     3361 acgaggtcga agtccgccgg ggcgaggagg ggaaggccga actccgcttg gtcaagcgcc
     3421 ggcagcgctg atggccctgc gccgccgtcg ccgccgtccg ctgtcgagga cggaactccg
     3481 cgcactcttt gggcggttgc gccaggccaa cgatccggcg gcacgggagg cgttgatcct
     3541 ggcctaccac agcctggcgg cctacctggc cggaaagttt gtggaccgcg gggaaccgct
     3601 cgaggatctc gtgcaggtcg cgcagatcgg gctgatcaac gcggtcgacc ggtacgatcc
     3661 cacccggggg atcgagttca ccaccttcgc cacgcccacg atcgtcggag agatccggcg
     3721 gcactttcgc gacaaactgc cgtcgatccg cattccccgg aggctgcggg aggtccatca
     3781 caccctgatg cggtccgtcg agagcctctc ccagcgcgtc gggcgctccc ccacgatccc
     3841 ggaactggcc gaagcgaccg gcatcccgtt cgacgtcgtg ctcgaggccc tggaagccgg
     3901 ccgcgcgtac gtcccggtct ccctcgacgc cggcggtccg gaggaggacg gcgaccccgc
     3961 aacgctgctg gcgtcgctcg gccgcgagga cgaagcactc caggggctgg aggaccaggc
     4021 cacgctggcg tgggcgatcg gccggttgcc cgagcgtgag cgggagctcg tgtcgctgcg
     4081 gttcttcaag cggctctcgc agggccaggt cgcacgccgt ctcggcatct cgcagatgca
     4141 cgtctcccgg ctccagcgcg cggccctcgc gcggctgcgc gcgctcatcg tccgcgggcc
     4201 cgctcacccg cactcctagc cggcgctcgg atgcccgatc tggcgacagc ggcggagatc
     4261 gcggagcgcc ttcgggcggc cgggttcgag acgtatctcg ttggtgggtg cgtccgcgac
     4321 atgctgctcg ggcgcgagcc cgcggactac gatatcgcca ccgccgccaa gcccgaggag
     4381 atccgggcgc tgttcccccg cacgatcgat gtcggggccg ccttcggcgt gatccgggta
     4441 ctggctccca atggggagta cgaggtggcc acgttccgca ccgaaggccc gtatctcgat
     4501 gggcgccatc ccgcctcggt gcaatacgcg accgtgcgcg aggacgtggc gcggcgggac
     4561 ttcacggtga acggactgct gtacgacccc aaggccgccg aggtcctcga cttcgtcggc
     4621 ggccgcgccg acctggccgc ccggcggatc cggacgatcg gggagcccgc ggcccggttc
     4681 gccgaagatc gtctccggat gctccgcgcc gtccggctgg ccgccgaact ggactttgag
     4741 atcgcgccgg aagtcaccgc cgccgcgcgc caactcgcgc
//
LOCUS       DASQGH010000515         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13761, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000515 DASQGH010000000
VERSION     DASQGH010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13761"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="VEW91_10130"
     CDS             complement(<1..1688)
                     /locus_tag="VEW91_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91978.1"
                     /translation="MKATILASLLAAVLLPPAFGGPAAPPHPSLPGLPNPKVMLGDPG
                     RFGGVFLDAQVSDPRTFNPILAQETSSTGPLGGLFDGLIEDNGETTETEPALAESWTT
                     SQDGRTWTFVLRKGLRWSDGVPVTADDVAFTFQVIYDKQIPNSQQDILTVAGKPIQVT
                     KVDDQTVRFRTAEPFGPFLRSIGVGILPRHKLEAPYKAGKFNQTWGVNTPPRELVGTG
                     SYIMTEYRPAQRISYVRNPYYWKADLAGHRLPYIERLVLTIVPDQNAHRLLFQQGADD
                     SYGVRPREYAEFKRGEQAGRYTVYDGGPSFGTEFLTFNQNPRSGLPEYKLRWFQNQKF
                     RQGVAYAVDRQAIADQVYAGHAIPQYGPESPANKFFFNPTVMRYPYDLDKAASTLAEG
                     GFKKGGDGVLRDGDGHPVQFIITTNADNPDRVAIANIVRQDLEKLGMQVTLAPEAFNT
                     MVNKLVESFKWEALVIGLTGGIEPHNGQNVWKSSGSLHMWNPKEPSPATPWEVQIDRY
                     FNLGATTVDQNRRKAYYDKFQEIIAEQAPVVYTTIPNAYVAVRNKFGNIKYTAFG"
     gene            complement(1727..>3118)
                     /locus_tag="VEW91_10135"
     CDS             complement(1727..>3118)
                     /locus_tag="VEW91_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYM91979.1"
                     /translation="NILEFERDYPDARVVTLEQNYRSTRRILDAAAGLVRHNPHRHTK
                     LLWTENAEGDPVTLFEAFDGYDEARYVGDLIQSHRAAGGHAGEIAILYRTNAQSRQFE
                     EMFLRLGIPYQIVGALRFYERAEIKDLLAYLRLAYNPTDDASLRRVLNVPRRGIGDGT
                     LRRLEGAAQARGTTLWDGLRSAEEAGVGPQHRRAIAEFVGLIDDLAKYATDHAAREVL
                     ARAIDATGYRRMLEAEATDEAYGRIENLDELLAVAEEVGVTTGEATLEAFLEHLALIT
                     DVDTWQERMDRVTLMTLHSAKGLEFPVVVLAGLEEGLFPHVRSMEEDAGVEEERRLCY
                     VGMTRAKHRLVLTYARQRAAFGTSRPGLPSRFLAEIPADLLARAATPKTATNDWPEDR
                     PVPDVAQGDLVRHKTFGAGRVLEVDGEGPRAIITVRFDSAGTKRLALGYAPLEVLRDS
                     SPRRPNIQSGPHS"
BASE COUNT          418 a         1052 c         1110 g          538 t
ORIGIN      
        1 ccgaacgccg tgtacttgat gttcccgaac ttgttgcgca ccgccacgta ggcgttcggg
       61 atggttgtgt acacgaccgg ggcctgctcg gcgatgatct cctggaactt gtcgtagtac
      121 gccttccgcc gattctggtc gacggtggtg gcccccaggt tgaagtaccg gtcgatctgg
      181 acctcccacg gggtcgccgg gctcggctcc ttagggttcc acatgtggag gctgccggag
      241 gacttccaga cgttctgccc gttgtgcggc tcgatcccgc cggtgaggcc gatcaccagc
      301 gcttcccact tgaaggattc gacgagcttg ttgaccatcg tgttgaacgc ctcgggggcg
      361 agggtcacct gcatcccgag cttctccagg tcctggcgga cgatgttggc gatcgccacg
      421 cgatccgggt tgtcggcgtt cgtggtgatg atgaactgga ccgggtgccc atcgccatcg
      481 cgcagcaccc cgtccccccc cttcttgaac ccgccctcgg cgagcgtaga agcggccttg
      541 tcgaggtcgt acgggtaccg catgacggtc gggttgaaga agaacttgtt ggccgggctc
      601 tccggcccgt actggggaat cgcgtggccg gcgtagacct ggtcggcgat cgcctgccgg
      661 tcgacggcat aggcgacccc ctggcggaac ttctgattct ggaaccaccg cagcttgtac
      721 tccggcaggc cgctccgggg gttctggttg aaggtgagga actcggtgcc aaagctgggg
      781 ccgccgtcgt agacggtgta gcgcccggcc tgctcccccc gcttgaactc ggcgtactcc
      841 cggggccgga cgccgtagct gtcgtcggcc ccctgctgga agagcaggcg gtgcgcgttc
      901 tggtcgggga cgatcgtgag caccagccgc tcgatgtagg gcaggcggtg gccggcgagg
      961 tcggccttcc agtagtacgg gttgcgcacg tacgagatgc gctgcgcggg tctgtactcg
     1021 gtcatgatgt aggacccggt gcccacgagc tcccgcgggg gcgtgttcac cccccaggtc
     1081 tggttgaact tcccggcctt gtagggtgcc tcgagcttgt ggcgggggag gatccccacc
     1141 ccgatcgacc ggaggaaggg accgaacggt tcggcggtcc ggaaccggac ggtctggtca
     1201 tcgaccttcg tgacctggat cggcttgccg gccacggtga ggatgtcctg ctggctgttc
     1261 gggatctgct tgtcgtagat cacctgaaag gtgaacgcga catcgtccgc cgtcacggga
     1321 acgccgtcgc tccaccggag ccccttgcgc agcacgaagg tccaggtgcg cccgtcctgg
     1381 ctcgtggtcc acgactcggc cagggcgggc tcggtctcgg tcgtctcgcc gttgtcctcg
     1441 atcagcccgt cgaacaggcc cccgagcggg cccgtggagg aggtctcctg cgccaggatg
     1501 gggttgaagg tccggggatc gctgacctgc gcgtcgagga aaacgccccc gaaccgcccg
     1561 gggtcgccga gcatcacctt ggggttgggg aggcccggga ggctcgggtg cggcggcgcc
     1621 gccgggccgc cgaacgccgg cggcagaagg acggcggcaa ggagactggc caggatggtc
     1681 gccttcacgg tacgacggtt cctcctcgcg cgcggggcgt gccggttcac gagtgcgggc
     1741 cgctttggat attcggccgc cggggggagg agtcccgcag gacttccagc ggggcgtagc
     1801 ccagcgccag ccgcttcgtt ccggcgctgt cgaaccgcac ggtgatgatc gcgcgcggcc
     1861 cctcgccgtc cacctcgagc acccgcccgg ccccgaaggt cttgtgccgg acgagatccc
     1921 cctgggccac gtcgggcacc ggccgatcct cgggccagtc gttggtcgcg gtcttcggcg
     1981 tggcggcgcg ggccagcagg tcggcgggga tctcggccag gaagcgcgac gggaggccgg
     2041 ggcgggaggt gccgaacgcg gcccgctggc gggcgtaggt gaggaccagg cggtgcttcg
     2101 cccgggtcat cccgacgtag cagagccggc gctcttcttc cacgccggcg tcctcctcca
     2161 tcgagcggac gtgggggaac agcccctcct ccaggccggc cagcacgacc accgggaact
     2221 ccagcccctt ggcgctgtgg agcgtcatca gcgtcacccg atccatccgc tcctgccagg
     2281 tgtccacgtc ggtgatgagc gccaggtgct cgaggaacgc ttccagggtc gcctccccgg
     2341 tcgtcacccc gacctcctcc gcgaccgcga gcagttcgtc gaggttctcg atcctgccgt
     2401 acgcctcgtc cgtggcctcc gcctcgagca tccgccggta cccggtcgcg tcgatcgcgc
     2461 gggcgagcac ctcgcgcgcc gcgtggtcgg tcgcgtactt ggccagatcg tcgatgaggc
     2521 ccacaaactc ggcgatggcc cgccggtgct gcggccccac cccggcctcc tcggcgctcc
     2581 gcaggccgtc ccacagggtc gtcccccgcg cctgcgcggc gccctcgagc cggcgcaggg
     2641 tgccgtcgcc aatcccccgc cgcgggacgt tgaggacccg ccggaggctc gcgtcgtcgg
     2701 tcgggttgta ggccaggcgg aggtaggcga ggagatcctt gatctcggcc cgttcgtaga
     2761 accgcagcgc ccccacgatc tggtacggga tcccgagccg caggaacatc tcctcgaact
     2821 ggcgggactg ggcgttggtc cggtagagga tggcgatctc cccggcgtgg ccgcccgcgg
     2881 cgcggtggga ctggatcagg tccccgacgt accgggcttc atcgtacccg tcgaaggctt
     2941 cgaacaacgt gacggggtcg ccctccgcgt tttccgtcca caggagcttc gtgtgccggt
     3001 gggggttgtg ccggacgagt ccggcggcgg cgtccaggat ccggcgggtg gagcgatagt
     3061 tctgctccag cgtcacgacc cgcgcgtccg gatagtcgcg ctcaaactcg aggatgtt
//
LOCUS       DASQGH010000516         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8051, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000516 DASQGH010000000
VERSION     DASQGH010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8051"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VEW91_10140"
     CDS             complement(<1..1105)
                     /locus_tag="VEW91_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HYM91980.1"
                     /translation="MAGSTDRTRVTLTAIKADVGSIGGHTLPSAEMLKAVSPLMEAQR
                     GKLLIDFRVTHTGDDICLTMTHRHGVEAEPVHRLAYEVFLAAGAVAERQGLYGAKQDL
                     LKSEFSGNIQGMGPGVAEMEIVERAAEPFMVLTADKTEPGAFNHLLYLTFCDPMFCSG
                     LLLSPDVGKGFTFTIVDVNYTEAEREITLQAPEELYDLAALLRDTGRFVIKSIHSRAH
                     PDEQAASLSTTRLKNIAGRYVGKDDPVAIVRTQKIFPATEEFGLAFSRVPFVAGDCRG
                     SHNMPLAPVPLNTDSSIFYCLPMVAAAGYSVRDGVLTGPIDFFKNPVWEVFREDAVRK
                     GAEMRAQGFFNPAMLGMEELEYGGIMERLKALDQ"
     gene            complement(1197..2177)
                     /locus_tag="VEW91_10145"
     CDS             complement(1197..2177)
                     /locus_tag="VEW91_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010996172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91981.1"
                     /translation="MADIQPDRVSGQPPQASAGPERSLLTVTPAGRPRYARTLWGDTW
                     RRFRRHRLAMAGAAAFLLFTAATLVGPVVYHVEINTIDFRASMAQPSGAHPFGTNDLG
                     QDMLARVLWGGRVSIAVGIAAMVMAITTGTLVGAIAGYFGRTTDMILMRIADVFISLP
                     QLPLLLLIIYLFRDRLSQLFGPQVGIFWLIVVVIGGLNWMHTARLIRAGFLSVKEKEF
                     IEAARGIGVGTVSQIFRHILPNVLSPVIVSATLSVGSAIIAESTLSFLGLGFPPDVPT
                     WGRLLYDAQNYLQLAPHMAIFPGLMICLTVLSVNYVGDGLRDALDPTRTF"
     gene            complement(2170..3114)
                     /locus_tag="VEW91_10150"
     CDS             complement(2170..3114)
                     /locus_tag="VEW91_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006617778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM91982.1"
                     /translation="MTAYITRRLLTSIPTLILISMVVFGILALAPGDPLGQFAANPDI
                     PPEVRQNILHQLGLDEPIPIRYVKWATSFLRGNWGYSFASHVEANGLILQRLPTDLWV
                     VGSAYLAAVVVAIPIGVLSAIKQYSLFDQIATTAAFIGFSLPTFFTGVLLIIIFSVKL
                     RWLPFIFNVQVTDPVGMLKQSVMPVAVLALFQSAGLMRFVRSSMLDNVSQDYVRTARA
                     KGVREQVVIIRHVLRNGLIPVVTLLALGLPAVFTGAVVTEQIFRVPGIGSLLITSIQN
                     SDTPVVMAITFVYAILVVMFNLVADVIYGLLDPRIKYG"
     gene            complement(3124..>4036)
                     /locus_tag="VEW91_10155"
     CDS             complement(3124..>4036)
                     /locus_tag="VEW91_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM91983.1"
                     /translation="EAMAKGGKGVVFTERGSGVEQIYSNQTDPNKEVDGQRSSVKAPH
                     AFLTDVKVRQALGLAIDRETLAKQLYGQTGDATSNVLTTPARLASRNTKLTLDVAKAN
                     QLLEEAGWQRGPDGVRSKGGVKLQVTYVTSVNTLRQKEQQIVKDGFTKIGVAATLQSV
                     DAGVFFSSSPGNNDTYPHFYRDLEMFTQTFSSPFPNQYMARYYSGDPAKDLAQKENNW
                     SGYNICRWVNKEYNQLYDQAQSELDPKKNDALWIKMNDLVVSQAISIPLIDRKFVSAR
                     STKLEVGENQSPFDSETRNIADWRRTG"
BASE COUNT          725 a         1318 c         1297 g          696 t
ORIGIN      
        1 gctggtccag cgccttgagg cgctccatga tgccgccgta ttcgagctcc tccatgccga
       61 gcatcgccgg attgaagaac ccctgggcac gcatttcggc gcccttgcgg accgcatcct
      121 cgcggaagac ttcccagacc gggttcttga agaaatcgat cgggcccgtc aagacgccgt
      181 cgcgcacgga gtagcctgcc gcggccacca tcggcaggca gtagaagatg ctggaatccg
      241 tattgagcgg gacgggcgcg agcggcatgt tgtggctgcc ccggcaatcg ccggcgacaa
      301 acgggacccg gctgaacgcc agcccaaact cctcggtcgc gggaaagatc ttttgcgtcc
      361 gaacgatcgc gacggggtcg tccttgccca cataccggcc ggcgatgttc ttgaggcgcg
      421 tggtgctcaa cgacgcggcc tgctcgtccg gatgggcccg ggagtggatg ctcttgatga
      481 caaaccgccc ggtgtcacgg agaagcgccg cgaggtcgta caattcctcc ggagcctgca
      541 gggtgatctc acgctccgcc tcggtgtagt tgacgtccac gatcgtgaac gtaaacccct
      601 tccccacgtc cgggctcagg agcagcccag agcagaacat cgggtcgcag aaggtgaggt
      661 acaggagatg gttgaacgcg cccggctcgg tcttatccgc ggtcagcacc atgaacggct
      721 cggctgcccg ctcgacgatc tccatctccg cgacccccgg ccccatcccc tggatgttgc
      781 cggagaactc gctcttgagg aggtcctgct tggcgccgta cagcccctgg cgctccgcca
      841 ccgccccggc ggcgaggaac acctcgtagg ccaaccggtg caccggctcc gcctccacgc
      901 cgtgccggtg ggtcatcgtc aggcagatat catcccctgt atgggtgacg cgaaagtcga
      961 tcagcaattt gccgcgctgc gcttccatca atggggaaac ggccttgagc atctcggcgc
     1021 tgggaagggt atgccccccg atgctcccaa cgtccgcctt gatcgctgtg agagtgaccc
     1081 gggttcgatc cgtggaccct gccatcccaa cacctccagg agacgcctag aaaccccgcc
     1141 gggagcctcc gtctacagac gccctcgtgt gtctattata gccgtttgtg tgaccgctag
     1201 aaggtccggg tggggtcgag cgcgtctcgg agaccatccc cgacgtagtt gaccgagagc
     1261 accgtgaggc agatcatgag ccctggaaag atcgccatgt ggggcgcgag ctggaggtag
     1321 ttctgcgcgt cgtacaggag gcgcccccac gtgggcacgt cgggcgggaa ccccagcccg
     1381 aggaacgaca gggtcgactc ggcgatgatc gccgaaccga ccgacagggt cgcggagacg
     1441 atcacggggc tgagcacatt cggcaggatg tggcggaaga tctggctcac cgtgccgacg
     1501 ccgatcccgc gggccgcctc gatgaactcc ttttccttga ccgacagaaa gcccgcccgg
     1561 atcaaccgcg cggtgtgcat ccagttcagc ccgccgatca ccacgacgat cagccagaag
     1621 atgcccacct gggggccgaa gagctggctc aggcggtctc ggaacaggta gatgatcagc
     1681 aggaggagcg gcagctgcgg cagcgagatg aacacgtcgg cgatccgcat caggatcatg
     1741 tcggtcgtcc ggccgaagta cccggcgatc gcgcccacca gggtgccggt cgtgatcgcc
     1801 atgaccatgg ccgcgatgcc caccgcgatc gagacccggc cgccccacaa cacccgggcc
     1861 agcatgtcct gcccgaggtc gttggtcccg aacgggtgcg ccccggacgg ctgcgccatc
     1921 gacgcgcgaa agtcgatcgt gttgatctcc acatgataga ctacagggcc gaccagcgtc
     1981 gccgcggtga agaggaggaa cgccgcggcg cccgccatcg ccaggcggtg ccggcggaag
     2041 cggcgccagg tgtcccccca cagcgtgcgg gcgtaccgcg gccggccggc cggcgtcacg
     2101 gtgagcaggc tccgctccgg cccagcggac gcctgcggcg gctggccgga cacccggtcc
     2161 ggctggatgt cagccatact tgatcctcgg gtcgagcagg ccgtagatca cgtcggcgac
     2221 gaggttgaac atgaccacca ggatcgcgta gacgaaggtg atcgccatga cgaccggggt
     2281 gtcgctgttt tggatcgagg tgatcaacag cgacccgatc ccgggcaccc ggaagatctg
     2341 ctcggtgaca acggcgccgg tgaagacggc gggcagcccc agcgccagca gcgtcaccac
     2401 cgggatgagc ccgttgcgca gcacgtggcg gatgatgacc acctgctcgc gcacgccctt
     2461 ggcccgcgcc gtgcgcacgt agtcctggct cacgttgtcc agcatcgacg agcggacgaa
     2521 ccgcatcaac ccggccgact ggaacagggc cagcaccgcc accggcatca cggactgctt
     2581 gagcatgccc accgggtcgg tgacctggac gttgaagatg aacggcagcc accgcagctt
     2641 gacgctgaaa atgatgatca ggagcacgcc ggtgaagaac gtcggcaggg agaacccgat
     2701 gaacgccgcg gtcgtcgcga tctgatcgaa cagggagtac tgcttgatcg ccgacagcac
     2761 cccgatcggg atcgcgacaa ccaccgcggc caggtaggcc gacccgacga cccacaggtc
     2821 cgtgggcagc cgctgcagga tcaggccgtt cgcttccacg tggcttgcga acgagtatcc
     2881 ccagttcccc cgcaggaacg atgtcgccca cttcacgtac cggatcggga tgggctcatc
     2941 cagccccaac tgatgcagga tgttctgccg cacctcgggc gggatgtcgg ggttggccgc
     3001 gaactgaccg agggggtcgc ccggcgccag cgcgaggatc ccgaacacaa ccatgctgat
     3061 gaggatcagc gtgggaatgg aggtcaggag ccggcgggtg atgtaggcgg tcatcgtggc
     3121 gatctagccg gtgcgccgcc agtccgcgat gttccgcgtc tcgctgtcga acgggctttg
     3181 gttttccccg acttcaagct tcgtcgagcg cgccgacacg aattttcgat cgatgagcgg
     3241 aatggagatc gcctggctga ccacaagatc gttcatcttt atccagagag catcgttctt
     3301 cttcggatcc agctcggact gggcctggtc gtaaagctgg ttatactcct tgttcaccca
     3361 ccgacaaatg ttgtaaccgg accagttgtt ctctttctgc gcgagatcct tggccggatc
     3421 cccgctgtag tatcgggcca tgtactggtt ggggaacgga gagctgaatg tctgggtgaa
     3481 catctccagg tccctgtaga agtgcgggta ggtgtcgttg ttccctggag agctgctgaa
     3541 gaacacgccg gcgtccacgg actggagcgt cgcagcgacg ccgatcttgg tgaatccgtc
     3601 tttgacgatc tgctgctctt tctgccgaag ggtgttcacg ctggttacat aggtcacctg
     3661 cagcttcacc ccgcccttcg atctgactcc gtcgggaccg cgctgccacc cggcctcttc
     3721 cagcaactgg ttggccttgg cgacgtcgag cgtaagcttg gtattcctcg acgccagccg
     3781 cgccggggtt gtgaggacat tggacgtcgc atcccctgtc tggccgtaca actgcttggc
     3841 cagcgtctcg cggtcgatcg ccagtccgag cgcctgacgc accttcacgt cggtcaggaa
     3901 ggcgtgcgga gccttgaccg acgaccgctg cccgtcgacc tccttgttgg ggtcggtctg
     3961 gttgctgtag atctgctcca cgccagaccc tcgctcggtg aagacgacgc cctttccccc
     4021 cttcgccatg gcctcg
//
LOCUS       DASQGH010000517         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_41857, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000517 DASQGH010000000
VERSION     DASQGH010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41857"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VEW91_10160"
     CDS             <1..159
                     /locus_tag="VEW91_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HYM91984.1"
                     /translation="EDITVFGGGIIPDEDIPGLQAAGIAAVFTPGTSLEAIVAWVRDH
                     VKPRGVAA"
     gene            156..356
                     /locus_tag="VEW91_10165"
     CDS             156..356
                     /locus_tag="VEW91_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYM91985.1"
                     /translation="MRVVIRHQKREVEVAGRRRVREVLAELGINPETVLVIRGAQLLT
                     HDAQVGEEDTIELRPVVSGGGV"
     gene            356..1321
                     /locus_tag="VEW91_10170"
     CDS             356..1321
                     /locus_tag="VEW91_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM91986.1"
                     /translation="MRCQKCKGQASVEVRRHHAAFCNEHFIEFFDRQVERAIEGGQMF
                     TRDDRVLVVVSGGKDSLALWDVLRRLGYRTAGLHINLGIGEYSGESQRKTEAFAAGQG
                     GELLIADIPHDYGMSVEEFARATGRASCSACGLSKRYLFNKVALDEGFSVVATGHNLD
                     DEAAVLLGNLLHWQTESLARQAPVLPCTHPHLVKKVKPFYRIAERETAAYAIIRGIDY
                     IVEECPNSLGAKSLLYKEALNLLEHAVPGTKQSLYWGFLERGRPLVRSLDTPALRACL
                     YCEQPTTAEVCAFCRMTAHAAERMRLRRQGAPTLPLPDRPLTPAG"
     gene            1334..2140
                     /locus_tag="VEW91_10175"
     CDS             1334..2140
                     /locus_tag="VEW91_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HYM91987.1"
                     /translation="MSARPLGPGEPVLLIDRRRRRYMIQLRPGGVSDLRGGKVAHVAL
                     LGQEEGRTVLSSRGERLLVVRPTLAEFVLEMPRGAQIVYPKDLGAILIGADVFPGARV
                     LEAGIGSGALTMALLRAVGPGGRVVSYEIREEFARIAEQNIRRFLGAADTLVLRRQDV
                     YAGIVPEDTPLDRIVLDVPEPWRVAPHAARALAPGGIWLSYVPTVPQIVETVEILMRP
                     WNIEGLSVRPAHRMVAHTGFLTTARRIQNGGAEHVPNESAEEEPADPEDP"
     gene            2137..3213
                     /locus_tag="VEW91_10180"
     CDS             2137..3213
                     /locus_tag="VEW91_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYM91988.1"
                     /translation="MKIRGLQARPVIAPLEPPIRTASGHLLEAPLSLIDLATEEGVVG
                     RSYLFGYQAFTLGPLRDLVVALGQEVKGDAVAPLELDRKLRARMTLFGTRGLQGMALS
                     GIEMAAWDALAVASGVPLAAFLGGRPRPIPAYNSLGMIPPDEAADEAGKTVAAGFKGL
                     KIKVGWPTVAEDLAVVRAARRALPDDVALMIDFNQSLSTPEAIRRGQVLDGEGVAWIE
                     EPVRADDFGSAAEIARQVATPIQIGENFNGPWEMSLALGMKASDLVMPDVQQIGGVAG
                     WLKAAALAQMAGVPCSSHIFIEASAHLLAVTPTCHWIEYLDVAAPVLAEPLRPVDGMI
                     QAPARPGIGLAWNEDAVRRYQVGR"
     gene            complement(3220..3396)
                     /locus_tag="VEW91_10185"
     CDS             complement(3220..3396)
                     /locus_tag="VEW91_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91989.1"
                     /translation="MPGSMEKIRIEQHSSLGLLWLGGWLFTIGLLQLTFWKGVLAIVL
                     WPYYLGAAFSALRH"
     gene            complement(3416..4594)
                     /locus_tag="VEW91_10190"
     CDS             complement(3416..4594)
                     /locus_tag="VEW91_10190"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYM91990.1"
                     /translation="MRDVVILSAARTPIGRFLGGLAPVPAPRLGSVAIAEALRRAQVP
                     PARVDEVYMGNILAAGLGQAPARQASLYAGLPNTVPATTLNKMCGSGLKAVMLGTQAI
                     QAGDADVVVAGGMENMSAAPYLLDRARAGYRWGHATLVDSMIKDGLWDVYNDMHMGNC
                     AELLVREHRISREEQDAFARRSYTRALEATASGHFRREIAPVEVSGDHGTPEVVSEDE
                     EPRRFDSARIATLPPVFEERGTVTVANASSISDGAAAVVIAAAEVAGQLGARPLARIV
                     GQAVSATAPEWFTIAPAAAIKRLLDKVGWARDGVDLYEINEAYAAVVIAVARNVGLDM
                     DRINVHGGAVALGHPIGASGARILTTLLYAMHERDARRGIAAVCLGGGEAVSLAVERV
                     "
     gene            4760..>5323
                     /locus_tag="VEW91_10195"
     CDS             4760..>5323
                     /locus_tag="VEW91_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HYM91991.1"
                     /translation="MEPKLLVDQGAALLLGEERDGEWVVGGVGARRLAREHGTPLYVM
                     DETRLRANCRAYVTALREAYPNSQAFFASKALCCLATCRLAYDEGMGVDVVSVGEIHT
                     ALRAGVPASALMLHGNNKTAEELALAIASGVGRIGVDNFNDLDMLDAMTREAGRGVDV
                     LLRLTPGIEPHTHKAIITGGVDSKFGFG"
BASE COUNT          799 a         1854 c         1896 g          774 t
ORIGIN      
        1 gaggatatca ccgtgttcgg cggcgggatc atcccggacg aggacatccc ggggctccag
       61 gcggcgggga tcgccgcggt cttcaccccc gggacctcgc tcgaggcgat cgtcgcctgg
      121 gtgcgcgacc atgtcaagcc ccgcggggtc gccgcgtgag ggtggtgatc cgtcaccaga
      181 agcgcgaggt ggaggtcgcg ggccgccgcc gcgtgcgcga ggtgctggcg gaactaggaa
      241 tcaaccccga aacggttctc gtgattcggg gcgcgcagtt gctgacccac gacgcccagg
      301 tgggcgagga agacacgatc gagctccgac cggtcgtgtc cggaggcggc gtctgatgcg
      361 gtgccagaag tgcaaggggc aggcttcggt cgaggtgcgc cggcaccacg cggcgttctg
      421 caacgagcac ttcatcgagt tcttcgaccg ccaggtggag cgggcgatcg agggcgggca
      481 gatgttcacc cgcgacgacc gcgtgctcgt ggtcgtatcg ggcggcaagg acagcctggc
      541 gctgtgggat gtgctgcgcc ggctggggta ccggaccgcg gggctgcaca tcaacctcgg
      601 catcggcgag tattcgggcg aatcccagcg caagacggag gcgttcgccg ccgggcaggg
      661 gggggagttg ctcatcgcgg acatccccca cgactacggc atgagcgtgg aggagttcgc
      721 ccgcgccacg gggagggcca gttgctcggc gtgcgggctg agcaaacggt acctgttcaa
      781 caaggtggcg ctcgacgagg gcttttccgt ggtggccacc gggcacaacc tggacgatga
      841 agccgccgtg ctgctcggca acctcctgca ctggcagacg gagtccctgg cccggcaggc
      901 gccggtcctg ccctgcaccc atccccacct cgtgaagaag gtgaagccct tctaccggat
      961 cgcggagcgt gagacggcgg cgtacgccat catccgcggc atcgactata tcgtcgagga
     1021 gtgcccgaac agcctggggg ccaagagcct cctgtacaag gaggcgctca acctgctcga
     1081 gcacgcggtc ccgggcacga agcagagcct ctactggggc tttctcgagc gcgggcgccc
     1141 gctggtgcga tcgctggaca ccccggcgct gcgggcctgc ctgtactgcg agcaacccac
     1201 gaccgccgag gtgtgcgcgt tctgccggat gaccgcgcac gcagcggagc ggatgcgcct
     1261 ccggcggcag ggcgcgccga ccctccccct gcccgaccgc cccctgacgc ccgcaggcta
     1321 gggcgcgccc ctcgtgtcag cgcggccgct cggaccaggc gagccggtgc tcctgatcga
     1381 ccgccggcgg cgtcgctaca tgatccagct gcggccgggg ggggtgagcg acctccgcgg
     1441 ggggaaggtc gcgcacgttg cgctcctcgg gcaggaggag ggccggaccg tgctgtcctc
     1501 ccgtggagag cgcctcctgg tcgtccgccc cacgttggcc gagttcgtgc tggagatgcc
     1561 ccgcggcgcc cagatcgtct accccaagga cctgggggcg atcctgatcg gcgccgacgt
     1621 gttccccggg gcgcgggtgc tcgaggcggg gatcggatcc ggagccctga cgatggcgct
     1681 gctccgggcc gtggggccag gcggccgggt cgtgagctac gaaatccgcg aggagttcgc
     1741 ccggatcgcc gagcagaaca tccgccgctt cctcggcgcc gccgacacgt tggtcctgcg
     1801 acggcaggac gtctacgcgg ggatcgtgcc cgaggacacc cccctcgaca ggatcgttct
     1861 cgacgtgccc gaaccgtggc gcgtggcccc ccacgccgcc cgcgccctcg ccccgggggg
     1921 catctggctc tcctacgtgc ccaccgtccc ccagatcgtg gagacggtgg agatcctgat
     1981 gcgtccgtgg aatatcgagg gcctgagcgt gcgtcccgcg caccggatgg tcgcccacac
     2041 gggctttctg acgaccgccc gcaggatcca gaacgggggc gcggagcacg ttccgaacga
     2101 atccgcggag gaagagcctg cggacccgga ggacccatga agatccgcgg cctgcaggca
     2161 cggcctgtca tcgccccgct cgagcctccc atccgcaccg cctcggggca cctcctggag
     2221 gccccgctgt cgctcatcga ccttgcaacc gaggagggcg tcgtggggcg ctcctacctc
     2281 ttcggctacc aggcgttcac gctgggcccg ctgcgcgacc tcgtcgtggc cctcggccag
     2341 gaggtgaagg gcgacgcggt ggcacccctg gagctcgacc gtaagctgcg cgcccgcatg
     2401 acgctgttcg gcacgcgcgg gctgcaaggg atggccctct cgggcatcga gatggcggcc
     2461 tgggacgcac tcgcggtcgc ctccggcgtg ccgttggcgg cgttcctggg cggccgcccc
     2521 cggccgatcc ccgcctacaa cagcctgggg atgatcccgc ccgacgaggc ggccgacgaa
     2581 gcgggcaaga cggtcgcggc gggcttcaag ggactcaaga tcaaggtcgg ctggcccacc
     2641 gtcgccgagg acctcgccgt cgtgcgcgcg gcgcgcaggg cgctgccaga cgacgtcgcc
     2701 ctcatgatcg acttcaacca gtcgctctcg acgcccgagg cgatccgccg gggccaggtc
     2761 ctcgacggcg aaggcgtggc ctggatcgag gagccggtgc gcgccgacga cttcgggagc
     2821 gccgcggaga tcgcccgcca ggtcgccacg ccgatccaga tcggcgagaa cttcaacggg
     2881 ccgtgggaga tgtcgttggc gctcgggatg aaggcgtcgg acctcgtcat gcccgatgtc
     2941 cagcagatcg gcggggtcgc cggctggctc aaggcggcgg cgctggcgca gatggccggc
     3001 gtgccgtgct cgagccacat cttcatcgag gcgagcgcgc acctgctggc cgtcaccccc
     3061 acctgccact ggatcgagta cctcgatgtg gcggcgccgg tgctggccga gcccctccgg
     3121 ccggtggatg ggatgatcca ggcgcctgcg cggccgggga tcggcctcgc gtggaacgag
     3181 gacgcggtgc ggcggtatca ggtcgggcga taggatagat cagtgccgca gggcgctgaa
     3241 cgccgcccca agatagtacg gccacaacac aatcgcgagg acacccttcc aaaaagtcag
     3301 ctggagaagc ccgatggtga agagccatcc cccgagccac aggagtccca gagaactgtg
     3361 ctgctcgatt cttattttct ccatgctccc aggcatcctc catcctttct gatcattaca
     3421 cgcgctccac ggccaggctc acggcctcgc cgccgccgag gcacacggcc gcgatcccac
     3481 gccgggcgtc ccgctcgtgc atggcgtaca ggagggtcgt gaggatccgt gccccgctgg
     3541 ccccgatggg gtggccaagc gcgacggcgc cgccgtggac gttgatccgg tccatgtcca
     3601 acccgacgtt gcgggccacg gcgatcacga ccgcagcata cgcttcgttg atctcgtaca
     3661 ggtccacccc gtcccgggcc cacccgacct tgtcgagcaa ccgcttgatg gcggcggccg
     3721 gagcgatggt gaaccactcg ggcgcggtgg cggagaccgc ctgtccgacg atccgggcca
     3781 gagggcgggc ccccagctgc cccgccacct ctgcggcggc gatcaccacc gcggccgcgc
     3841 cgtcgctgat cgacgaggcg ttcgcgacgg tcaccgtccc tcgctcctcg aacacggggg
     3901 gcagcgtggc gatgcgcgcg gagtcaaagc gacggggttc ctcgtcttcg ctcacgacct
     3961 ccggggtgcc gtgatccccc gacacctcca ccggggcgat ctcacggcgg aaatggcccg
     4021 acgcggtcgc ctcgagggcc cgcgtatagg agcggcgggc aaacgcgtcc tgttcttccc
     4081 gggagatccg gtgctcgcgc accagcaact cggcgcagtt gcccatgtgc atgtcgttgt
     4141 agacatccca cagaccgtct ttgatcatcg aatccacgag cgtcgcgtgt ccccaccggt
     4201 atccggcccg agcccggtcg aggaggtagg gggcggcact catgttctcc atgcccccgg
     4261 ccacgacgac gtccgcgtcg ccggcctgga tcgcctgcgt cccgagcatc acggccttga
     4321 gccccgaccc gcacatcttg ttgagggtgg tggcggggac ggtgttgggc agcccggcgt
     4381 acagggacgc ctggcgcgcc ggcgcctggc ccagcccggc ggcgaggatg ttgcccatgt
     4441 acacctcgtc gacccgcgcc gggggaacct gcgcccgcct gagcgcctcg gcgatggcga
     4501 cgctccccag ccggggagcg gggaccggtg ccaagccccc gaggaaccgg ccgatcgggg
     4561 ttcgagcggc gctcagaatc acgacgtccc gcatagcgat ccctccccca cgggtacgtt
     4621 ggatgccccc cgatccattc cctggggccg agtgcacccg acggcctgtc gcatttgaca
     4681 ctcgcgtcgc acccatgcta tcttgaagtc ggcgccagcc tggttgtccg ggcgggcgcc
     4741 atcgtgtagg ggggccaggg tggaacccaa actgctggtg gatcaaggcg cagccctgct
     4801 cctgggcgag gagcgggacg gcgagtgggt cgtggggggc gtcggcgcca ggcgcctggc
     4861 ccgcgagcac gggacgcccc tgtacgtcat ggatgagacg cgcctaaggg ccaactgccg
     4921 cgcctacgtg acggccctgc gggaggccta tcccaactcc caggccttct tcgccagcaa
     4981 ggccctctgc tgcctggcga cctgccgcct cgcgtacgac gagggcatgg gggtggatgt
     5041 cgtctccgtc ggggagatcc ataccgcgct ccgggccggg gtgcccgcga gcgcgctgat
     5101 gctgcacggc aacaacaaga cggcggaaga gctggcgctc gcgatcgcct ccggcgtcgg
     5161 ccggatcggg gtggacaact tcaacgacct cgacatgttg gacgcgatga cgcgcgaggc
     5221 ggggcggggg gtggacgtgc tgctcaggct cacccccggc atcgagcccc acacgcacaa
     5281 agcgatcatc accggggggg tcgacagcaa gttcgggttc ggg
//
LOCUS       DASQGH010000518         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44728, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000518 DASQGH010000000
VERSION     DASQGH010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44728"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VEW91_10200"
     CDS             complement(<1..1082)
                     /locus_tag="VEW91_10200"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HYM91992.1"
                     /translation="MSVLDDVRRSKARDPSGMLEIALALPRQVREGWALGRAAKLPRL
                     AEAPEHLVVCGMGGSAIAGDLLAGYLAPRLPIPITVVRGYEVPGFVGSRSVVIAASYS
                     GATEETLSAVAQAERAGATIFAITSGGQMAPAAGRAGVMVPAGLVPRAALGYMMLPML
                     AALERWELAEPSATEVEEAAAALDTLAADCAPDVPALRNPAKRLAEQLADRIPAVYAA
                     SPGLEAAARRWKTQFNENSKTLATWSVFPELNHNETVGWGAPPHLAGQFAVVVLLGGA
                     EPPRLVQRIRLTTDLALGPAAGVHEVRARGAGRLARLLSLVMMGDLVSVYLAYLRRVD
                     PTPTEIIDTIKQRLREVRRSSRNSTTP"
     gene            1208..1609
                     /locus_tag="VEW91_10205"
     CDS             1208..1609
                     /locus_tag="VEW91_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91993.1"
                     /translation="MGGERRAPDRNRPVRRSAIDIRALLAHREYIKTTAYLLPRSAKI
                     QVLELMERLRRDRTDRRGTAAEGVPSPLPQMTGGELVREINWRIGTLPPKDAEVVTRH
                     IHRLCRWRAGRSRERRDGGGPLPPPPRRTGR"
     gene            1609..>2068
                     /locus_tag="VEW91_10210"
     CDS             1609..>2068
                     /locus_tag="VEW91_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91994.1"
                     /translation="MAASGSRRPRILLLHYTGPPVVGGVEAVLARHARLLAREGLDVH
                     VLVGRGAPLGGGVTFHRRALLDSRHPRVLAAARELEDGWVTPAFSALAARIRDDLLES
                     LAGIDVCVVHNALTLHKNLALTVALHDVVARGSRAQVVAWCHDLAWTNPQY"
BASE COUNT          294 a          768 c          738 g          268 t
ORIGIN      
        1 ggcgttgtcg agttacggga ggatcggcgg acctcgcgca accgctgctt gatcgtgtcg
       61 atgatctcgg tcggcgtcgg gtcgaccctg cggagatacg ccaggtagac gctcacgagg
      121 tcgcccatca tcaccagcga gagcagacgg gcgagccggc cggcaccgcg cgcgcgaacc
      181 tcgtgcacgc ccgcggccgg gccgagcgcc agatcggtcg tgaggcggat gcgctgcacc
      241 aggcgcggcg gctcggcccc gcccagcagg accaccacgg caaactgacc ggccagatgg
      301 ggcggcgccc cccacccgac cgtctcgttg tgattcagct ccgggaaaac gctccaggtc
      361 gccagggtct tgctgttctc gttgaactga gtcttccagc ggcgcgccgc ggcctcgagc
      421 ccgggcgagg cggcgtacac cgcggggatc cgatccgcca gctgctcggc gaggcgcttc
      481 gccgggttgc gcagcgcagg aacgtcgggc gcgcaatccg cggcaagggt gtcgagcgct
      541 gcggccgcct cctccacttc cgtcgcggag ggctccgcga gttcccaccg ctccagggca
      601 gccagcattg ggagcatcat gtacccgagc gcggcgcggg ggaccaggcc cgccggcacc
      661 atcacgcccg cccgccccgc cgccggcgcc atctgtcccc cggaggtgat cgcgaagatc
      721 gtcgcgccgg cgcgctccgc ctgggcgacg gccgacaggg tttcctcggt cgcgccggag
      781 taactcgcgg caatcacgac cgagcgcgaa cccacaaacc ccgggacctc gtatccccgc
      841 accaccgtga tgggaatcgg caatctgggc gccaggtatc cggcgagcag gtcgccggcg
      901 atcgcggacc cgcccatccc gcagacgacg agatgctccg gcgcctcggc cagccgggga
      961 agcttggcgg cgcggccgag ggcccacccc tcacgcacct gccggggcag cgccagcgcg
     1021 atctccagca tcccggatgg atcgcgcgcc ttcgaccgcc tgacgtcgtc cagcacggac
     1081 atacccgagg attcgccgct aggacggcct ctccccgccc gggtgcccgg cggcgcaggg
     1141 agagctccgg gcgggcccga aggaaccatc cgtttctcag gcgagacgca tccgggagac
     1201 ctcagggatg ggcggtgagc ggcgcgcgcc ggaccggaac cgaccggtgc gcaggtcggc
     1261 gattgatatc cgggccctgc tcgcccaccg ggagtacatc aagacgaccg cctacctgct
     1321 gccccgcagt gcaaagatcc aggtgctcga gctcatggag cggctccggc gggaccggac
     1381 ggaccggcgg ggcacggcgg ccgagggtgt cccctcaccc cttccgcaga tgaccggcgg
     1441 ggaactggtg cgcgagatca actggcggat cgggacactg ccccccaaag atgccgaggt
     1501 cgtgacccgc cacatccacc ggctgtgccg ctggcgggcg gggcggagtc gcgagcgacg
     1561 cgacggcggg ggcccgctgc cgcctccccc gcgcaggacg ggccgttgat ggctgcgtcc
     1621 ggctcccgtc ggccgcgcat cctcctgctc cactacaccg gtccgcccgt cgtgggcggg
     1681 gtcgaggccg tgctcgcccg gcacgcgcgg ctcctcgccc gggagggact cgacgtgcac
     1741 gtcctggtcg ggcgcggggc gccgctgggc ggcggggtca ccttccaccg ccgtgccctc
     1801 ctggactccc gccatccgcg cgtcttggcc gccgcgcgcg aactcgagga cgggtgggtc
     1861 acgccggctt tctcggcgct cgccgcgcgg attcgggacg acctcctgga gtcgctggcc
     1921 ggcatcgatg tgtgtgtggt gcacaacgcg ctgacgctgc acaagaacct cgcgctcaca
     1981 gtcgcgctgc acgacgtcgt ggcgcggggc agccgcgcgc aggtcgtggc atggtgccac
     2041 gacctcgcgt ggacaaaccc gcagtacc
//
LOCUS       DASQGH010000519         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33456, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000519 DASQGH010000000
VERSION     DASQGH010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33456"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..539)
                     /locus_tag="VEW91_10215"
     CDS             complement(171..539)
                     /locus_tag="VEW91_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM91995.1"
                     /translation="MQILRVLAVLKRPAQRTASRGIAKGRRAPPAPVVPTGDLINLLS
                     NERLRLYRQVSGRPLPRAVRVRLGEIVRELDRLWDLRRREMVTFPVLNGSDEIPKPRS
                     VAPAKSPDRRPPGVPVPASA"
     gene            complement(509..>2982)
                     /locus_tag="VEW91_10220"
     CDS             complement(509..>2982)
                     /locus_tag="VEW91_10220"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HYM91996.1"
                     /translation="VIPIKDDSIEAIFQWMKEAARTYSLGGGVGGDISVLRPAGAPVN
                     NAARTSTGSISFMELMSLTTGTIGQSGRRGALMITIADHHPDVLEFVRIKRNLNRVRF
                     ANISVRLSDAFMRAVEADEPWELHFENERVDVRRTVRAREVWDELVRGARDFAEPGVI
                     FWDAIKRWSTSEYNGMNVVTTNPCSEIPLEPYGACCLGNLNLAAFVNDEFSPEAQVDW
                     PHLEQALRLATRFLDDVLDYNADKHPLPEQREASLYSRRIGVGFTGLGDMLCKLRLKY
                     DTDEAIEFVDRFFDRAKNIIYDESVNLAREKGVFPGYNRERHLQGRFLQTLDPAVLER
                     IQEHGLRNVALLTVPPVGSGASLAGTTSGIEPIFDLGYTRRSESLSQETFKVSHPLVK
                     DYMERFGLASEEELPAFFVTAHEIRPEMRVRMQAAIQKHIDHSISSTVNCPGETTAED
                     VAKIYFLAWQLGCKGITVYRANSRQNILTAGTRRSVQAAGSAAAPQAQAAPAPVEAAQ
                     PMKPLEPLKVPQPAEPSAVVEASRPMEPLAPVKAPQPAEPSAVVEASRPIEPLAPVKA
                     PQLAEPSAVVEASRPIEPPAPVKAPQPAEPPPVTKVPWPAEPPAVREAPSPHQGRPST
                     RPRPKVTTGRTERIETPRGRIYVTINEDSQGVCEVFVQSLDVEADAIGRIASLALRTG
                     ADPRHVIEQLWRVQSREVAIDRSNDGTVVRVTTVAQGVALAIGRALYGPGFRPDLVFP
                     MADRLPVPRSSNGGANGQEAAASRESEGGGTADPGALVPEYAAPAEDFQQPLLTFAGV
                     CPDCGSSLIYENGCSSCRSCGYSRC"
BASE COUNT          453 a         1008 c         1031 g          490 t
ORIGIN      
        1 gggccgtagt tctgtcgccc ccaaaatttc tcctgccccg gacgggcgca cccgcatcga
       61 tccggcagga ggaatgggcg gcggagcgag ggttccgcgg cctgcgcgag cgtggatatc
      121 cgagtggata tccgagcggt cggaacctga tcctaactaa gtaggccgag ctaagccgag
      181 gcaggaacgg ggaccccggg gggccgtcgg tccggcgatt tggcgggggc tacactgcgg
      241 ggtttgggga tttcatccga gccattgagt acggggaagg tgaccatctc ccggcgccgc
      301 aggtcccaga ggcgatcgag ctcgcgaacg atctccccga gccgtacccg gaccgcccga
      361 gggagggggc gccccgaaac ctgccggtac agccgcaggc gttcgttcga gagcaggttg
      421 atgaggtccc cggtcggcac cacgggcgcc gggggcgccc gccttccctt cgcgatcccc
      481 cgacttgcgg tgcgctgcgc cgggcgcttc agcaccgcga gtacccgcag gatctgcacg
      541 agctgcagcc gttctcgtag atgagggaac tgccgcagtc cgggcagacg ccggcgaagg
      601 tcaggagcgg ttgttgaaag tcctccgccg gggccgcgta ctccggaacg agcgccccag
      661 gatcagcggt cccacctccc tcgctctccc ggctggcggc ggcctcctgg ccgttggcgc
      721 ccccgttcga ggaccggggg acgggcaatc gatcggccat gggaaagacg aggtccgggc
      781 ggaacccggg accgtagagg gctcggccga tggccagggc gacgccctgg gccaccgtgg
      841 tgacccgcac gaccgtccca tcattggacc ggtcgatcgc cacctcccga gattgcacgc
      901 gccagagctg ctcgatcacg tgccgggggt cggcgccggt gcgcagggcc agcgacgcta
      961 tccgaccgat cgcatcggcc tccacgtcga gcgactgcac aaacacctcg cagacgccct
     1021 gactgtcctc gttgatcgtc acgtagatcc gcccgcgcgg ggtttcgatc cgctcggtcc
     1081 gtccggttgt taccttgggc cgcggccgcg tggacgggcg cccctgatgg ggcgaggggg
     1141 cttctcggac cgccggcggt tccgcgggcc aggggacctt cgttacgggc ggcggctccg
     1201 cgggctgggg agccttcacc ggcgccgggg gctctatcgg ccgggacgcc tccaccactg
     1261 ccgagggctc cgcaagctgg ggagccttca ccggcgccag cggctctatc ggccgggacg
     1321 cctccaccac cgccgacggc tccgcgggct ggggagcctt caccggcgcc aggggctcca
     1381 tcggccggga cgcttccacc accgccgacg gctctgcagg ctgggggacc ttcagtggct
     1441 ccaggggctt catcggttgg gcagcctcca ccggcgccgg cgccgcctgc gcctgcgggg
     1501 cagccgcgga tcccgccgcc tggacggacc ggcgcgtccc cgcggtgagg atgttctggc
     1561 gcgagttggc ccggtagacc gtgatgccct tgcaccccag ctgccaggcg aggaagtaga
     1621 tcttggcgac gtcctccgcg gtggtctcgc ccgggcagtt gacggtggac gagatcgagt
     1681 gatcgatgtg cttctggatc gcggcctgca tgcgcacccg catctccggc cggatctcgt
     1741 gggcggtgac gaagaacgcc gggagctctt cttcgctcgc caggccgaac cgctccatat
     1801 agtccttgac cagcgggtgg gagaccttga aggtctcctg cgagagcgac tcactccgcc
     1861 gcgtgtaccc caggtcgaag atcggctcga tgccgctggt ggtcccggcg agcgacgccc
     1921 cgctcccgac cgggggcacg gtcaggagcg cgacgttccg caggccgtgc tcttggatgc
     1981 gctcgagcac ggccggatcg agcgtctgga ggaaccggcc ctggaggtgc cgttcccggt
     2041 tgtaccccgg gaagacgccc ttctcccggg cgaggttgac gctttcatcg tagatgatgt
     2101 tcttggcccg gtcgaagaac cggtccacga actcgatggc ctcgtccgtg tcgtatttga
     2161 ggcggagctt gcacaacata tccccgaggc cggtaaaccc gaccccgatg cgccgcgagt
     2221 agaggctggc ttcacgctgt tcagggagcg gatgcttgtc ggcgttgtag tccagcacgt
     2281 cgtcgaggaa gcgcgtcgcc agccgaagcg cctgctccag gtggggccag tccacctggg
     2341 cctcggggga aaactcatcg ttgacaaatg cggcgagatt tagattacca aggcagcatg
     2401 ctccataagg ctcaagcggg atctctgagc aaggattagt ggtgacgacg ttcatgccgt
     2461 tgtactcgga tgtgctccac cgcttgatcg cgtcccagaa gatgacgccg ggctcggcga
     2521 aatcccgggc gccccgcacc agttcgtccc acacctcccg ggcccggacc gttcgccgga
     2581 cgtcgacccg ctcgttttcg aagtggagct cccacggttc atcggcttcc accgcgcgca
     2641 tgaacgcgtc gctgagccgc acgctgatat tcgcaaaccg gacgcggttg aggttgcgct
     2701 tgatcctcac gaactccagc acgtcggggt ggtgatctgc aatggtgatc atgagcgcgc
     2761 cccggcggcc gctctgcccg atcgtccccg tggtcaggga catcaactcc atgaaggaga
     2821 tcgagccggt gctggtccgg gccgcgttgt tcaccggcgc gcccgcgggc cggaggacgc
     2881 tgatgtcgcc gcccacgccg ccgccaagcg agtaggtccg cgccgcctcc ttcatccact
     2941 ggaagatcgc ttcaatcgag tcatccttga tgggaatgac gt
//
LOCUS       DASQGH010000520         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47681, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000520 DASQGH010000000
VERSION     DASQGH010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47681"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VEW91_10225"
     CDS             <1..1479
                     /locus_tag="VEW91_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYM91997.1"
                     /translation="IEECAEMDVLCTDKTGTLTQNRPTVAALRPYPPHSEAELLRLAA
                     LACDEATQDPIDLAVLEAAKARDGLRAPVERVRFIPFDPVTKKSGAAFYQAGAAQYVF
                     KGAPQVIGPLTAVPAERLTRDVETLAQEGARVLAVAAGPEGALELAGLVALHDPPRSD
                     SKALIDSLHQLGVRTLMVTGDSPATAKVVAAQVGIGTRASPRGGLDQVPEGQILEYDV
                     FARILPEGKFRLVQALQRTGHIIGMTGDGVNDAPALRQAEVGIAVAQATDVAKSAASL
                     VLTSPGLVDVVAAVETSRRIHQRMLTYTLNKIVKTFEISLLLSLGLLVGRVFLTTPLL
                     IVLLLFANDFVTMSIATDRVSFGRVPDRWNVRPLVVAALGVAAFLLLFSFAIAWVGLR
                     VLRLPLASLQTLMFVWLVLSGQATVYLVRERGHFWRTWPSRWMMASTAGDLIGVSLLA
                     ARGWLMAPIPPLVLMALLGVALLYVVTADQLKVPLFRSCGMR"
     gene            complement(1579..1723)
                     /locus_tag="VEW91_10230"
                     /pseudo
     CDS             complement(1579..1723)
                     /locus_tag="VEW91_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422179.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            1862..2959
                     /gene="hisC"
                     /locus_tag="VEW91_10235"
     CDS             1862..2959
                     /gene="hisC"
                     /locus_tag="VEW91_10235"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYM91998.1"
                     /translation="MTLPRARSALAALEPYIPGLSVEEVTERFHITTVTKLGSNENPL
                     GLSPRVREALIRSLDRIHHYPDGDCTRLRRRLADRLGLTPAHVCCANGVDNVLTCVGL
                     AYLDAGERCVLGAPTYTAYRSLVRLLGAVPVEVPLRDWRYDVRATAEAAPGAKAVIVC
                     NPNNPTGTILRHDELQALVERIPSDVLVVLDEAYAEWADDPGFPDAVALLRRHPNLVV
                     LRTFSKIYGLAGLRVGYAIAAPEIVGSLNQVREPFPVDRLAQAAAEAVLDDEPYVRAS
                     FENNRQGKAFLAKAFAGLGLRHLPSQANFILVDLGTPAAEAAARLLERGVIIRPGAMW
                     GLPTWARVTIGTPEENARLTAALSAVLGKTR"
     gene            2991..3476
                     /locus_tag="VEW91_10240"
     CDS             2991..3476
                     /locus_tag="VEW91_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYM91999.1"
                     /translation="MTASRITELIKAYAEGPRLLESAVAGIPRAELHFTPGPEHWSIH
                     ENVVHVADSELVGVTRMRYVLAEPGAVLVPFDQEKWARVMDYRSQSLEGALALFRATR
                     AATTEVLRRAPTEAWEHAGINTEAGPQTLEWLVEHFADHVHYHLRTIAKRRTQYAQAR
                     R"
     gene            3518..5161
                     /gene="hutU"
                     /locus_tag="VEW91_10245"
     CDS             3518..5161
                     /gene="hutU"
                     /locus_tag="VEW91_10245"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYM92000.1"
                     /translation="MRAPRGTRLSCRGWPQEAALRMLMNNLDPEVAERPADLVVYGGT
                     GRAARNWACFDGLVRALRSLGGDETLLVQSGKPVGVFRSHPAAPRVLISNAMLVPAWA
                     TWDEFRRLEALGLTMYGQMTAGSWIYIGTQGIIQGTYETYAAVARQHFGGTLRGRLVL
                     TAGLGGMGGAQPLAITMNEGVGLVVEADPARIERRARAGWVDEATQSLDAALHLVQQA
                     CREGRPRSIALLGNAAEVYREIVRRGVRVDVVTDQTAAHDLLEGYLPEGLSVQEAAEL
                     RRRAPEEYLRRAEASVVRHVEAILSLQKRGAVAFDYGNNIRAQAHRAGASDAFAFPGF
                     VPAYVRPLFCEGRGPFRWVALSGDPSDIRKTDEAVLRLFPEQEALTRWIRLAGERVPF
                     QGLPARVCWLGYGERARFGLAINEMVARGELQAPIVIGRDHLDSGSVASPYRETEAMA
                     DGSDAIADWPILNALLNAVGGATWVAVHHGGGVGIGYSLHAGMVIVADGLPGTDRRLE
                     RVLTTDPGLGILRHADAGYPLAIEAARRAGLPLPMPERT"
     gene            5161..>5818
                     /locus_tag="VEW91_10250"
     CDS             5161..>5818
                     /locus_tag="VEW91_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM92001.1"
                     /translation="MRWAYLGRQVLCGQTLLPDHAVVVRDGDIEAIVPAGSPALREVG
                     VEDWGAVLIVPGTVNTHTHSFQSLVRGISDDLPFFRWRDQGIYRIAPRLGPDEIYAGA
                     LLTFAEMLRCGVTTVADFFYVHAGGYANVEAVIRAARDLGIRLVLVRAMYDWPGAPAV
                     YQETVDEAVRRCEDLLSRYAGDPMVTVCPAPHSPHAASPEMIAAGARLAARAGTRWHI
                     H"
BASE COUNT          822 a         2076 c         2057 g          863 t
ORIGIN      
        1 atcgaagagt gcgcagaaat ggacgtcctc tgcacggaca agaccggcac gcttacgcag
       61 aaccggccga ccgtcgcggc cctccgcccc taccctccgc atagcgaggc tgagctgctc
      121 cgcttggccg ccctcgcctg cgacgaggcc acgcaggacc cgattgacct ggcggtcctg
      181 gaggcggcga aggcccggga cggactccgg gcgccggtcg agcgggtgcg gtttatcccc
      241 ttcgatcccg tgaccaagaa atcgggggcg gcgttctatc aggcgggcgc ggcgcagtac
      301 gtgttcaaag gcgcccctca ggtgatcggc ccgctgaccg ccgtgcccgc ggagcggctc
      361 acccgggatg tggaaaccct ggcccaggag ggggcccggg tcctggccgt cgccgccggg
      421 cccgaggggg ccctggagct ggcggggctg gtggccctgc acgatccgcc gcgctccgac
      481 tccaaagcgc tgatcgacag cctccaccaa ctcggggtgc gcacccttat ggtaaccggc
      541 gacagcccgg cgaccgcgaa ggtcgtcgcc gcccaggtcg gcatcggcac gcgcgcgtcc
      601 ccgcgcgggg gcctggacca ggtcccggag gggcagatcc tcgagtatga cgtgttcgcc
      661 cggatcctcc cggagggcaa gttccgattg gtgcaggcgc tccagcggac gggacacatc
      721 atcggcatga ccggcgacgg cgtgaacgac gccccagccc tcaggcaggc cgaggtcggc
      781 atcgccgtcg cgcaggcaac ggacgtggcc aagtcggccg ccagtctcgt cctcacgagc
      841 cccggcctcg tggatgtcgt cgcggcggtg gagaccagcc gccgcatcca ccagcggatg
      901 ctcacctaca ccctgaacaa gatcgtcaag accttcgaga tctcgcttct gttgagcctg
      961 ggcctgctcg tggggagagt cttcctcacc accccgctcc tcatcgtcct gctgctcttc
     1021 gcgaacgatt tcgtcacgat gtcgatcgcc accgaccggg tctcgtttgg gagagtcccc
     1081 gaccggtgga acgtccgtcc gctggtcgtc gccgcgctcg gcgtcgctgc cttcctcttg
     1141 ctcttctcct tcgcgatcgc ctgggtgggc ctgcgggtcc tccggctgcc cctcgcatcc
     1201 ctccagaccc tcatgttcgt ctggctggtc ctgagcgggc aggccactgt ctacctcgtg
     1261 cgtgagcgcg ggcacttctg gcggacgtgg ccgagccggt ggatgatggc aagcaccgcg
     1321 ggggatctca ttggagtcag cctgctggcc gctcgaggct ggctgatggc gccgatcccc
     1381 ccgctggtct tgatggcgct gctgggggtg gcgctcctct acgtcgtcac cgccgaccag
     1441 ctcaaggtgc cgctgttccg atcctgtggc atgcgctgat cccctccgtg gttcacgaat
     1501 tcggcgtgtc ggccgcccaa gctccggatg catcgggcac gcagaagaag gctctcgact
     1561 aaattgcggg gcaagaaaat ccggcacgcg actaccggcc accaaagtga cggcccccgc
     1621 ccaggctaac gcgacccgct cccgcggact gtagaacggc gtctcgcccc aaaccggcag
     1681 ggcatagaac cgctgttcgc tttctccgcg ggcgccttga gtccgcggcc ggtccccccg
     1741 cgcaccacgg cgcgcgccgt gcgggatcga gccagcgccc cgtcgaatcg agcgagtgcg
     1801 acgccgccgg ctggtgcaca cggcgtttca gcggcgtgcc gtcaggaagg agcgattggt
     1861 ggtgaccctc ccgcgcgcgc gcagcgccct cgctgcgctc gagccctaca tccccggact
     1921 gtcggtagaa gaggtgaccg agcggttcca catcacgacc gtgaccaagc tcgggagcaa
     1981 cgagaacccc ctcgggctct ccccccgggt ccgcgaagcg ctgatccgat cgctcgatcg
     2041 gatccaccac tatccggacg gcgactgcac ccggctgcgc cggcggctgg cagaccggct
     2101 gggcctcacc ccggcgcacg tgtgctgcgc gaacggcgtc gacaacgtcc tgacctgcgt
     2161 gggcctcgcg tatctcgatg ccggggagcg ctgcgtcctc ggcgcgccga cctacaccgc
     2221 ctaccggagc ctggtcaggc tcctcggtgc cgtcccggtc gaggtgccgc tccgcgactg
     2281 gcggtacgac gtccgggcga ccgcggaggc cgctcccggg gccaaggccg tgatcgtctg
     2341 caaccccaac aaccccacgg gcacgatcct gcgccacgat gagctgcagg cgttggtgga
     2401 acggatcccg tcggacgtcc tcgtcgtcct ggacgaggcc tacgcggaat gggcggacga
     2461 ccccggattc cccgacgcgg tggccctcct ccgccgccac cccaacctcg tggtcctgag
     2521 aaccttctcc aagatctacg gcctcgccgg gctccgcgtc ggctacgcca tcgccgcgcc
     2581 cgagatcgtc ggctcgctga accaggtgcg cgagccgttt cccgtcgacc ggctcgcgca
     2641 ggccgccgcc gaggcggtcc tggacgacga gccctacgtg cgggcctcgt tcgagaacaa
     2701 ccgccagggg aaggccttcc tggcaaaggc gtttgcgggg ctcggcctcc ggcacctgcc
     2761 gagccaggcc aacttcatcc tggtggatct tggaactccc gccgccgagg cggcggcccg
     2821 cctgctcgag cgcggcgtga tcatccggcc gggcgcgatg tggggactgc cgacgtgggc
     2881 gcgcgtcacg atcgggacgc cggaggagaa tgcccgcctc accgcagcgc tctcggcggt
     2941 gcttggaaag acgcgttgat gcagccccaa tacgagagga ggagactgcg atgacggcga
     3001 gtcggatcac ggagctgatc aaagcatacg ccgaaggccc ccgcctgctc gaatcggcgg
     3061 tggcggggat cccgcgggcc gagctccact ttaccccggg gcccgagcac tggagcatcc
     3121 acgagaacgt cgtccacgtc gccgacagcg agctggtcgg tgtcacgcgg atgcgatacg
     3181 tgctggcgga accgggagcc gtcctggtcc cgttcgacca ggagaagtgg gcccgggtca
     3241 tggactacag atcgcagtcg ctggagggag ccctcgccct gttccgggcc acccgggccg
     3301 ccaccacgga ggtcttgcgg cgcgcgccca ccgaggcctg ggagcacgcc gggatcaaca
     3361 ccgaggccgg gccgcaaacg ctcgagtggc tcgtcgagca ttttgcggat cacgtgcatt
     3421 atcacctccg cacgatcgcg aagcgccgga cccagtacgc gcaggcaagg cgctgatcgc
     3481 tgtggaccgg acgccggaat cgggggggcc gcggggggtg cgcgcgcccc gggggacccg
     3541 gctgtcctgc cgggggtggc cgcaggaggc ggcgctccgg atgctcatga acaacctcga
     3601 tccggaggtg gccgagcgcc ccgccgatct cgtcgtctac gggggcaccg gacgggcggc
     3661 acgcaactgg gcgtgctttg atgggctggt gcgggccctg cggtccctcg gcggcgacga
     3721 gaccctcctc gtccaatccg ggaaaccggt gggcgtcttt cggtcacacc ccgccgcgcc
     3781 ccgggtcctg atcagcaacg ccatgctcgt cccggcctgg gcgacgtggg acgagtttcg
     3841 gcgactcgag gcgctcggcc tcacgatgta cggccagatg acggccggat catggatcta
     3901 catcggcacg caggggatca tccagggcac ctacgagacg tacgcggccg tggcccggca
     3961 gcattttggc ggcacgctgc gcgggcgcct cgtgctcacc gccgggctcg ggggcatggg
     4021 cggggcgcag ccgctggcga tcacgatgaa cgagggggtc ggcctggtgg tcgaggcgga
     4081 ccctgcccgg atcgaacgtc gggcgcgggc cggatgggtg gacgaagcca cccagagcct
     4141 cgatgcagcc ctgcatctcg tccagcaggc ctgccgggaa ggccggccgc gctcgatcgc
     4201 gctcctcggc aacgccgccg aggtgtaccg cgagatcgtc cggcgggggg tccgggtgga
     4261 cgtcgtgacc gaccagaccg ccgcccacga cctgctggaa ggctacctcc cggagggcct
     4321 gtccgtccag gaagccgcgg agttgcgccg gcgggccccg gaggagtacc tgcggcgggc
     4381 ggaggcgtcc gtcgtccggc acgtggaggc gatcctcagc ctccagaagc gcggggcggt
     4441 cgccttcgac tacggcaata acatccgcgc ccaagcgcac cgcgccggcg cctccgacgc
     4501 gtttgccttc ccggggtttg tgcccgccta cgtccggccg ctgttctgcg agggccgggg
     4561 cccgtttcga tgggtcgcgc tctcggggga tcccagcgac atccggaaga cggacgaggc
     4621 ggtgctgcgc ctcttccccg agcaggaggc gctgacccgc tggatccgcc tcgccggtga
     4681 gcgcgtcccg ttccagggcc tgccggcgcg ggtctgctgg ctcgggtacg gcgagcgcgc
     4741 ccggttcggt ctcgcgatca acgagatggt cgcgcgcggt gagctccagg cccccatcgt
     4801 gatcgggcgg gaccatctgg attcgggatc cgtcgcctcc ccgtaccggg agacggaggc
     4861 gatggccgac ggcagcgacg cgatcgcgga ctggccgatc ctgaacgccc tgctgaacgc
     4921 cgtcggcggc gccacatggg tggccgtcca ccacggcggc ggcgtcggga tcgggtactc
     4981 cctgcatgcg gggatggtga tcgtggcgga cggcctgccc ggcacggacc gccggctgga
     5041 gcgggtcctc acgacagacc ccgggctggg gatcctgcgc cacgcggacg ccgggtatcc
     5101 gctcgcgatc gaggcggcca gacgagcggg gctgccgctg ccgatgcccg agcggaccta
     5161 atgcggtggg cgtacctcgg ccggcaggtc ctctgcggcc agacgctgct ccccgaccac
     5221 gcggtcgtcg tgcgggacgg ggacatcgag gcgatcgtcc ccgccggctc tccggccctc
     5281 cgggaggtcg gggtcgagga ctggggcgcg gtcctgatcg tcccgggcac ggtgaacacg
     5341 cacacgcact cgttccaatc cctggtgcgc gggatctcgg acgatctgcc gttcttccgg
     5401 tggcgtgacc agggcatcta ccgcatcgcc ccccgcctcg ggccggacga gatctacgcg
     5461 ggagcgctct tgacgttcgc ggagatgctc cggtgcggcg tcaccaccgt ggcggatttc
     5521 ttctacgtcc acgccggcgg ctacgcgaac gttgaggcgg tgatccgggc ggcgcgggac
     5581 ctcgggatcc ggctcgtcct cgtccgcgcg atgtacgact ggccgggggc cccggcggtg
     5641 taccaggaga ccgtcgacga ggcggtgcgg cgctgcgagg atctgctcag ccggtacgcg
     5701 ggcgatccga tggtcacggt ctgcccggcc ccccacagtc cccacgcggc ctccccggag
     5761 atgatcgcgg cgggcgcgcg cctcgcggcc cgggccggca cccggtggca catccacg
//
LOCUS       DASQGH010000521         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47682, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000521 DASQGH010000000
VERSION     DASQGH010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47682"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VEW91_10255"
     CDS             complement(<1..251)
                     /locus_tag="VEW91_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYM92002.1"
                     /translation="MSIVRVEPFEELHKMRRQVDRLLEDIFPRSIVREWAVPELEWEP
                     AVEMFETENEVVVKAALPNIDPKQVDITVTNDAITLKGET"
     gene            complement(374..1456)
                     /gene="rtcA"
                     /locus_tag="VEW91_10260"
     CDS             complement(374..1456)
                     /gene="rtcA"
                     /locus_tag="VEW91_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HYM92003.1"
                     /translation="MIVLDGSRKSGSGTIVRSAAVLAALARRPLRVTNVRAKRAHPGL
                     QPQHLRAVETVASLSGGHLENARVGSREFALQASGCIRGGRYLVDIGTAGSTTMLALT
                     VLPLAAFADEPSRFEIRGGLFQDFAPSAFHLQHALLPLLGRMGLSAEVRMIRPGYVPR
                     GEGVLELSVRPVSGALRPLVLTDPGRVTRVWGIALSSHLHAQRVSERLANRCQERLAA
                     HGFGAEIQLHNDTTALQRGAALAVFAETDTACILAADRAGAPGRPAEAIADRVAEMLL
                     EDLASGATVDRHLGDQLVLYAALADGVSEFTVPNVTDHVDTNCWLVEEIVGAKVALAG
                     RALRIAGVGYRRPAAGNRKNWAGSSM"
     gene            complement(1453..2442)
                     /locus_tag="VEW91_10265"
     CDS             complement(1453..2442)
                     /locus_tag="VEW91_10265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYM92004.1"
                     /translation="MRLAFVGDVMLGRLVNRCLKRVPAEYPWGDTLPLLRTADAVFIN
                     LECVIADRGKPWPRKVFTFRTDSRNVEVLRTAHVTVASLANNHSLDYGAEALLDCLEI
                     LQRHGIWAVGAGATLGAARLPAVLAVCGARIAVIAVTDNEPEWQAGEETPGVFFVPFA
                     DKNPRLHDLFALVEDVRKASDLVIVSAHWGPNWGRTPLPEHVEAAHRLIDAGADVVFG
                     HSPHILRGIEIYRGRPILYSCGNFVDDYAVDEDERNDESGIFSIECDQQGVRRVLFTP
                     TVITRFQARLARGADWTRILGQMRTVCAGLGTDTREASEGLEISVSPVVMAER"
BASE COUNT          432 a          875 c          798 g          415 t
ORIGIN      
        1 gtctctccct tgagcgtgat cgcgtcgttc gtgaccgtga tatctacctg cttggggtcg
       61 atgttcggaa gcgccgcctt cacgaccacc tcgttctcgg tctcgaacat ctcaaccgcc
      121 ggctcccact ccagctccgg aacggcccac tcgcgcacta tactcctggg gaagatatcc
      181 tccaagagcc ggtcgacctg ccgacgcatc ttgtgcaatt cctcaaacgg ctccacgcgg
      241 acaatgctca tgatacctcc ctcctcttcg tgcctttctc atccttcccc aataataaga
      301 atatctccaa cgaagtatga aatgcatcgg gtggggaccc gatagaccgt cgggcgggag
      361 ccgcactctc gggtcacatc gaacttccgg cccagttctt gcggttcccc gcggccggcc
      421 tccggtagcc gaccccggcg attcggaggg cgcggccggc gagcgctacc ttagccccca
      481 cgatttcctc gacgagccag caattcgtgt cgacatggtc ggtcacgttt ggcaccgtga
      541 actcgctcac cccgtcggcc agcgcggcgt agagcacaag ctgatctccg aggtggcgat
      601 cgacggtcgc tcccgacgcg aggtcctcca ataacatctc ggccacgcga tcggcgatgg
      661 cctcggccgg gcgtcccggc gccccagccc gatcggccgc caggatgcac gcggtgtcgg
      721 tctccgcgaa cacggcaagc gccgcgccgc gctggagcgc ggtcgtgtcg ttgtgtagct
      781 ggatctcggc gccgaaccca tgtgcggcca ggcgttcctg gcatcggttg gccagtctct
      841 cgctcacgcg ctgcgcgtgg aggtgtgatg agagggcgat gccccatacc cgcgtcacgc
      901 gacccggatc cgtgaggacc agggggcgca gagcaccgga cacgggccgg accgagagtt
      961 ccaagacgcc ctcccccctc ggtacatatc cgggccggat catcctgacc tctgcggaga
     1021 gtcccatgcg gcccagcagc ggcagcagcg catgctgcag gtgaaaggcg gatggggcga
     1081 agtcctgaaa gagaccgcca cggatctcaa accgacttgg ctcatcggcg aacgctgcga
     1141 gcggcagcac ggtgagggct agcatcgtcg tggaaccggc ggtcccgatg tcgacgaggt
     1201 acctgccgcc gcggatgcag ccggacgcct gcagcgcgaa ctcgcgcgag ccgacccgtg
     1261 cgttttcgag atgcccgccc gagaggctgg ccaccgtctc caccgcccga agatgctgcg
     1321 gttgcagccc cggatgggcg cgcttcgcgc ggacgttcgt gacccgcagt ggacgccgcg
     1381 ccagggccgc gaggacagcc gcagagcgga cgatcgtgcc gctgcccgac ttccgggagc
     1441 cgtcgaggac gatcaacgct ctgccatcac caccggcgac accgagatct ccagcccctc
     1501 tgacgcctcg cgcgtgtcgg tacccaggcc ggcacacacg gtgcgcatct ggccaaggat
     1561 ccgtgtccag tcggccccac gggccagccg ggcctgaaac cgcgtgatga ccgtgggggt
     1621 aaacagcacc cgtcggaccc cttgctggtc gcactcgatg gagaagatcc ccgactcgtc
     1681 gtttcgctcg tcctcgtcga ccgcgtagtc atcgacgaag tttccgcaac tgtacaagat
     1741 cggccggccc cgatagatct ctatccccct caggatgtgc gggctgtggc caaacacgac
     1801 gtccgcgccg gcgtcgatga gccgatgggc ggcctcgacg tgttccggca ggggcgtacg
     1861 cccccagttc gggccccagt gggcggagac gatcaccagg tcgctcgcct tcctcacgtc
     1921 ttcgaccagg gcgaataggt cgtggagtct tgggttcttg tcggcgaagg gcacgaaaaa
     1981 aacaccgggg gtctcctccc cggcctgcca ctcgggctcg ttgtcggtga cggcgatgac
     2041 ggcaatgcgc gcaccgcaga cagcgaggac cgcgggcaac cgtgcggccc cgagcgtggc
     2101 accggccccg accgcccaga tgccgtgccg ctggagtatc tcgagacagt cgagcagcgc
     2161 ctcggctcca tagtccaacg agtggttgtt cgccagcgat gccaccgtga catgagccgt
     2221 cctgagcacc tcaacgttcc gtgagtcggt gcgaaaggtg aagaccttcc gcggccaggg
     2281 cttgccgcgg tcggcgatga cgcattcgag attgatgaag accgcgtcgg ccgtccggag
     2341 gagcgggagc gtgtcccccc atggatactc ggcgggcacc ctcttgaggc acctgttcac
     2401 cagccgcccc agcatcacgt cgccgacaaa agccagcctc atggagaggc tcccggcgcg
     2461 tggcacctcg gggaggacgt gtgttcggtg acttgagtca cacccaaccc cccggatcag
//
LOCUS       DASQGH010000522         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13796, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000522 DASQGH010000000
VERSION     DASQGH010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13796"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="argS"
                     /locus_tag="VEW91_10270"
     CDS             complement(<1..730)
                     /gene="argS"
                     /locus_tag="VEW91_10270"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYM92005.1"
                     /translation="MLTEELRAVIARAAERAVDSGDLPASATPIPSFEVEPPRDPRHG
                     DYAANLALVLARAAGRPPREVGAAILRHLPVPSEVVATAELAGQGFLNITLAPPCLHR
                     WLRRVHAEDRQFGRTDVGRGRRVLVEYVSANPSGPLHVGSGRGGAVGETIARLLEALG
                     YRVSREYYVNDYGNQMEALARSVEARYLEAIGRPTTFPDDGYQGAYVVEIAQRIAGEH
                     GAQLAEMERAARLSRIQAIALRAML"
     gene            complement(732..1700)
                     /gene="glpX"
                     /locus_tag="VEW91_10275"
     CDS             complement(732..1700)
                     /gene="glpX"
                     /locus_tag="VEW91_10275"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HYM92006.1"
                     /translation="MERTLALDIVKVTEAAAIAAARHMGEGQSEQADKAAVTAMHAAL
                     VELPIHGRIVIGEGERDEAPTLYTGEEVGSGEGFAVDVAVDPLEGTNLLAKGLPNAIA
                     ILAVTEKGGILHAPDMYMEKLVVPPQAVGKVDLRWPPERNLEVLAQALQRAISDITVV
                     ILDRPRHADLIAAVRKAGARIKLISDGDVAPAISVAVSGPGVHAVMGIGGAPEGVLTA
                     AALRCLGGEIQGRFWYRNDGERQRAKEMGIGDLDRVFRTKDLVRGNALVFSATGITDG
                     DLFRGVRLFGGGIRTHSLVMTASAHQIRFVDAIHIHDMPRVGVVRL"
     gene            complement(1715..>2143)
                     /locus_tag="VEW91_10280"
     CDS             complement(1715..>2143)
                     /locus_tag="VEW91_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYM92007.1"
                     /translation="GAVIGINTAIIPNAQGIGFAIPSDVVRVEMEQLIANGRVIRPWI
                     GVVYGGEVDPQSGRAYNLGTEHGVVVRQVEPAGPAARAGVQPGDIITAVNGERVDSWS
                     DFVRDVVAKKIGDTVALTIVRERAARTLSVTLAERPAEVR"
BASE COUNT          313 a          787 c          722 g          321 t
ORIGIN      
        1 ccagcatggc ccggagggca atggcttgga ttcgggagag gcgcgccgct cgttccatct
       61 ccgccaactg cgccccgtgc tccccggcga tccgctgcgc gatctcgacg acgtaggcgc
      121 cctgataccc gtcgtcagga aacgtcgtgg ggcggccgat cgcctccagg taccgcgcct
      181 cgaccgagcg ggcgagggct tccatctgat tcccgtaatc gttcacgtag tattcgcggg
      241 aaacgcggta gccgagcgct tcgaggaggc gggcgatggt ctcgcccacg gcaccccccc
      301 gccccgaccc cacgtgcagg ggccccgatg ggttggcgct cacgtattcg accagcacac
      361 gccggcctcg accgacatcg gtccgcccga actgccggtc ctcggcgtgc acgcggcgaa
      421 gccatcggtg caggcacggc ggcgccagcg tgatgttgag gaacccctgg ccggcaagtt
      481 cggctgtggc caccacctcg gacggcacgg ggaggtggcg gaggatcgcc gcgccgacct
      541 cccgcggcgg acggcccgcg gcccgcgcga gcaccaacgc aaggttcgcc gcgtaatcgc
      601 cgtggcgggg atcccgcggg ggctcgacct caaaggaggg gatcggggtg gccgaggcgg
      661 ggaggtcccc tgaatcgacc gcacgctccg cggcccgggc gatgaccgcc cgaagttcct
      721 cggtgagcac gttacagcct gacgacgccg acccgcggca tgtcgtggat gtggatggcg
      781 tccacaaatc gaatctggtg ggcggacgcc gtcatcacca gcgagtgggt gcggatcccg
      841 ccgccgaaca gccggacgcc gcggaacagg tcgccatcgg tgatgcctgt ggcgctgaac
      901 acgagggcgt tcccgcggac gaggtccttg gtccgaaaca cccggtcgag gtcaccgatg
      961 cccatttcct tcgcgcgctg ccgctccccg tcgttgcggt accagaaccg accctggatc
     1021 tcgccgccga ggcaccgcag cgccgcagcg gtcagcacgc cttcgggagc gcccccgatg
     1081 cccatgaccg cgtggacccc gggacccgag accgccaccg agatggccgg ggcgacgtcc
     1141 ccgtccgaga tcagcttgat gcgcgccccc gccttgcgga ccgcggcgat caggtccgcg
     1201 tggcggggcc ggtccaggat gaccaccgtg atgtccgaga tcgcgcgttg gagggcctgc
     1261 gccagcacct cgaggttgcg ctcggggggc caccgcaggt cgaccttccc gaccgcctgt
     1321 ggggggacca cgagtttctc catatacata tcgggggcgt gcaggattcc ccccttctcc
     1381 gtcacggcca ggatcgcgat ggcattgggg aggcccttgg cgagcaggtt cgtcccctcg
     1441 agaggatcga ccgcgacgtc aaccgcgaag ccctcgccgc tgcccacctc ctcgccggtg
     1501 tacagcgttg gggcctcatc ccgctccccc tcgccgatca caatccggcc gtggatcggc
     1561 agttccacca gcgcggcgtg catggcggtg accgcggcct tgtccgcctg ctcggactgc
     1621 ccttcgccca tgtggcgggc ggcggcgatc gccgcggcct cggtcacctt gacgatgtcg
     1681 agcgcgagtg tccgctccat gggacccacc acggctaccg gacctccgcc ggccgctcgg
     1741 ccagggtgac ggagagcgtg cgcgcggcgc gctcccgcac gatggtcagc gcgaccgtgt
     1801 ccccgatctt cttggcgacc acgtcgcgaa cgaaatcgct ccaactgtcg acccgctccc
     1861 cgtttaccgc ggtgataata tcgcccggct gcacgcctgc gcgcgcggcc ggcccggcgg
     1921 gctcgacctg gcgcaccacg accccgtgct cggtcccaag attgtacgct ctcccggact
     1981 gcggatcgac ctccccgccg tacacgacac cgatccaggg gcggatgacc cggccgttgg
     2041 cgatgagttg ctccatttcg acccggacca cgtcgctggg aatcgcgaac ccgatcccct
     2101 gcgcgttggg gatgatggcg gtgttgatcc cgatcaccgc ccc
//
LOCUS       DASQGH010000523         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_21963, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000523 DASQGH010000000
VERSION     DASQGH010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21963"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1261)
                     /locus_tag="VEW91_10285"
     CDS             complement(38..1261)
                     /locus_tag="VEW91_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92008.1"
                     /translation="MNRRILAVLSYGHLATDLSQGALPALLPVFKSLFHLSYGGVGFI
                     VLMANISSSVIQPAFGFLSDRARVTWLMPLGALLAGLGITMAVLSPHFAVMVVWVLIS
                     GLGVAAFHPEGYRFAGLAAGERRATGMSYFSAGGNIGYGFGPAVATLALSLANVRGMV
                     YLIAFSISAAILLWRTVGTRQRERLEAEWVHPAAPPAARARAGPLSRKGSAGILVLLI
                     AFVVLRSWVSVGTASFIPLFFTGIRHLDPRYGGWLVSLFLGAGAVGTLLGGAAADRFG
                     RRTLLIVSMAILPPLLLMLTRTNGIVTMMAATIAGMATVSTFSVAMVMAQDLMPERIG
                     MISGLIIGFAVGMGGVGVTLLGAIADRWGLQTAMDITALLPTVALVIALALPAGRLVR
                     AAPEPQVAGETLDYR"
     gene            1448..>2040
                     /locus_tag="VEW91_10290"
     CDS             1448..>2040
                     /locus_tag="VEW91_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HYM92009.1"
                     /translation="MVAVALLALLLSPAPVLGQAAVHPELLAQVSGARAYEHVLALSK
                     QIGPHVAGTPEDRTSAAYIARQLSNDGYAVEWQPFQFPFFAVRAVTLAVLSYPGLTLH
                     PRAMLYSPSSPAGGITADLVDVGLGRPEDVRATALAGKIALILRGTLPFREKAQNAAD
                     AGAVAAIVYNSRPEEFGGRVGRDAKIPVVAHSGAGGL"
BASE COUNT          340 a          703 c          716 g          281 t
ORIGIN      
        1 cgaatgcgtc atgccgccct ccccagggac ctcccgctta tctatagtcc aacgtttctc
       61 ctgccacctg tggttcgggc gcggcacgga ccaaccgccc ggccggaagc gcgagcgcga
      121 tgacgagggc gacggtgggc agtagcgcgg taatgtccat cgcggtctgc aggccccacc
      181 ggtcggcaat cgcgccgagc agcgtgacgc cgaccccacc catgcccacc gcgaagccga
      241 tgatcaggcc cgagatcatt ccgattcgct cgggcatcag gtcctgcgcc atgaccatcg
      301 cgaccgagaa cgtgctgacc gtagccatgc ccgcgatcgt tgccgccatc atcgtcacga
      361 tgccgttcgt ccgcgtgagc atcagcagga gcggcggcag gatggccatc gagacgatga
      421 ggagcgtccg gcggccaaag cggtccgcgg cggcgccgcc cagcagggtg cccaccgcgc
      481 ccgctccgag gaacaggctc acgagccatc caccgtaccg cggatcgaga tgacggatcc
      541 cggtgaagaa caacgggatg aagctcgccg tgccgacgga cacccaggaa cgcaggacga
      601 cgaacgcgat gaggaggacg agaatccccg ccgagccctt ccggctcaac ggcccggctc
      661 tggccctggc cgcgggggga gcggcggggt ggacccattc ggcctccagg cgctcgcgct
      721 gccgcgttcc caccgtcctc cagaggagga tggctgcgga gatgctgaag gcgatgaggt
      781 agaccatgcc gcgcacgttg gcgaggctga gggccagcgt ggccacggcg ggcccaaacc
      841 cgtacccgat gttgccaccc gcggaaaaat aggacatgcc ggtcgcgcgc cgctcgccgg
      901 cggcgagccc ggcgaaccga tagccctcgg ggtggaacgc cgctacaccg agtccggaga
      961 tgagcaccca cacgaccatc accgcgaagt gcggggacag gaccgccatc gttatcccaa
     1021 ggcccgcgag cagcgcgccg agcggcatca accaggtcac cctggcccgg tcgctgagga
     1081 atccgaacgc cggctggatg accgaggaac tgatgttggc catcagaaca ataaacccca
     1141 ccccgccgta ggagaggtgg aacagcgact tgaacaccgg gaggagtgcc ggaagtgcac
     1201 cctgggaaag atcggttgcc agatggccgt agctcaggac ggccagaatg cggcggttca
     1261 cggtcaggga ttcgacggtg agcggggccg ccccttggtc ggacggagcc gcctgggcgg
     1321 ggcgaggggc ggtgggggtt gactttttcc tgcgccgtcc gtaccgtggg accacaccac
     1381 cgggctcagg gggtgcgcgt tgatgcgggg acaggccgtc tttgctagtc ggcggggcac
     1441 ggcgggcatg gtcgctgtgg ccctcctcgc cctgctgctg tcgccggcgc ccgtgctcgg
     1501 tcaggctgcg gtgcacccag agctgctggc gcaggtcagc ggcgcgcggg cctacgagca
     1561 cgtgctggcg ctctccaagc agatcgggcc gcacgtcgcc ggcacccctg aggatcggac
     1621 ctcggccgcc tacatcgccc gccagctctc gaacgacggg tacgcggtgg agtggcagcc
     1681 cttccagttc ccgttcttcg cggtgcgcgc cgtcacgctg gcggtgctct cctaccccgg
     1741 gctcacgctg cacccgcgcg ccatgctgta ctccccgtca tcccccgccg gcgggatcac
     1801 ggcggatctg gtcgacgtcg gcctcggccg ccccgaagac gtccgggcca cggcgcttgc
     1861 ggggaagatt gcgctgatcc tgcgcggtac cctcccattc cgcgagaagg cccaaaacgc
     1921 ggcggatgcc ggggcggtgg ccgcgatcgt ctacaactcg aggcccgagg agtttggggg
     1981 ccgcgtgggg cgcgacgcga agatccccgt cgtcgcccac tcgggcgcgg gagggttgga
//
LOCUS       DASQGH010000524         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44771, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000524 DASQGH010000000
VERSION     DASQGH010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44771"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..2006)
                     /locus_tag="VEW91_10295"
     CDS             complement(252..2006)
                     /locus_tag="VEW91_10295"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HYM92010.1"
                     /translation="MGEQTEAHFDSLALAAVAREIQACRRARFAGVRQPSRDALVVSL
                     RTGGAVRHLLCSIHPKLARIHFVSPPEETERLTPFGLLLRSRLVEARLEDVEQPPFDR
                     IIRLAFDALEGRVWLIAEIMGRHSNVVLADDRVVIGALKVVTAQMSRRRPVLPGRPYV
                     RPPADRPTPDAITPAALAAVLTGDLPLARRISQAVLGVSPTLAREVAVRAGLDPQMPA
                     HAALGAADLMLGVLREIAGDVARGAFAPVLYAEDDRVVAFAPFPLRVYERLVPIPVGT
                     MSEALDRYYRDAGDRGSRATLLDERRAALRRQVAGALDKRGRALQESREILAASREAE
                     RYRVMGELLLTYAHGAKPGAVRLRVPDHTAGGAEIEIPLDPALTPSQNAQRYFRRYAK
                     GRAAVRAVPARIAALEDEVRALRDALVQIDAAASPDDLWEIHTDLAASRLVGRSPRGR
                     PAARTGPRRFATPDGAVILVGRSARENDRITFRESGPDDLWFHARGIAGAHVILKAGG
                     RPSEASIRAAAEAAAYYSEGRAGGQVAVDCVARRHVRKPRGAAPGAVTYTGERTLRVT
                     PQLPPPGPTSGRRSGTAS"
     gene            2062..2928
                     /locus_tag="VEW91_10300"
     CDS             2062..2928
                     /locus_tag="VEW91_10300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HYM92011.1"
                     /translation="MTGFGSGDLTTVAGRYVVEARSLNHRFLEVLVRLPRDLAPLEER
                     IRALVQGRILRGRVEIAIIRDNYGKRVRTAKVDVDLAKAFTSALNDLKQALDLPGTLD
                     LSMVVGLPDLIKVEEPKEDLEEIWPAIAVGIGQALGRLVAMREGEGGRLARDLEERIE
                     RVGHRIGDVERRAPLVVKEHAARLSRRIAELLGSVPVDEGRLATEVAMFADRCDIAEE
                     ITRFRSHLAQMRQTLGAEGAVGRTLEFIAQELGREVNTMGSKANDLEIARAVIAIKGE
                     LESLREQIQNVE"
     gene            2943..>3064
                     /locus_tag="VEW91_10305"
     CDS             2943..>3064
                     /locus_tag="VEW91_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HYM92012.1"
                     /translation="METRLINIGFGNIVAANRIIAIVSPDSAPIKRIIQEARDKG"
BASE COUNT          465 a         1056 c         1119 g          424 t
ORIGIN      
        1 agatcgaggc cgatgccatc gtggaagagt aacactaccc cgctctatgg aggcgagagt
       61 cgcccaggca ttctcgtcgg atcgtgaccc cgggatgccg cgcatgtcca tccagcccta
      121 ttgatccggt gggggatccg cacgctggta atgccgccca gcgcaaagtc gggaccgaaa
      181 gaccgataca tcgccgcccg gggcgtacct ccgcgaagcc gaggtgttgg cccctccgca
      241 cgtcgtgatc gtcatgaggc ggtgcccgac cggcgtcccg aggtcgggcc gggcggcggg
      301 agctgcggcg tgacccggag ggtccgctcc cccgtgtacg tgacggcccc gggggcggcg
      361 ccgcggggtt tccggacatg cctgcgcgcg acgcaatcca cggcgacctg gcccccagcc
      421 cggccttcgc tgtagtaggc cgccgcctca gcggcggcgc ggatgctcgc ctcgctgggc
      481 cgcccaccgg ctttgaggat cacgtgggcg ccggcgatgc cgcgggcgtg gaaccacagg
      541 tcgtccgggc cggactcgcg gaacgtgatc cggtcgttct cgcgcgcgct tcggccgacc
      601 aggatgaccg ccccgtcggg ggtggcaaat cgccgcggcc ccgtgcgcgc cgcgggccgg
      661 ccgcgcggac tgcgcccaac caggcgggat gcggcgaggt ccgtgtggat ctcccacagg
      721 tcatcgggag acgcggccgc atcgatctgc acaagggcgt cccggagggc ccgcacctca
      781 tcctcgaggg ccgcgatgcg ggccgggacc gcccggacgg ccgcccggcc tttcgcgtac
      841 cgccgaaaat accgctgcgc gttctggctc ggcgtgagcg cgggatccag cgggatctcg
      901 atctcggcgc ctcccgcggt gtgatcgggg acccggagcc gcacggcccc gggcttcgcc
      961 ccgtgcgcgt aggtcaggag cagctcgccc atcacccggt accgctcggc ctcccggctc
     1021 gcggcgagga tctcgcggct ctcctggagc gcccgccccc gtttgtcgag cgcgccggcc
     1081 acctggcggc gcagtgcggc gcgccgctca tcgaggagcg tggcccggct gccccgatcg
     1141 ccggcgtccc gatagtaccg gtcgagcgct tcgctcatcg tccccacggg gatcgggacg
     1201 aggcgctcgt acacgcgcag ggggaacggg gcgaacgcca cgacccgatc gtcctccgcg
     1261 tacaggaccg gggcgaacgc gccacgggcg acgtcgccgg cgatctcgcg caggacgccc
     1321 agcatcaggt cggccgcccc cagggcggcg tgcgcgggca tctgcggatc caggcccgcc
     1381 cgaaccgcga cctcccgggc cagcgtcggg ctcaccccga gcaccgcctg cgagatccgc
     1441 cgtgcgagcg ggagatctcc tgtgaggacg gccgcgagcg ccgcgggcgt gatcgcgtcc
     1501 ggcgtcggcc ggtccgccgg aggacgcacg taggggcggc ccggcaggac gggtcgccgg
     1561 cgcgacatct gcgcggtcac gaccttgagc gcgccgatga ccacccggtc gtccgccagg
     1621 accacgttgc tgtgtcgtcc catgatctcc gcgatcagcc acacccgacc ttccagcgca
     1681 tcgaaggcga gccggatgat ccggtcgaag ggcggctgtt cgacgtcctc caggcgcgct
     1741 tcgacgagcc ggctccggag gagcaacccg aacggcgtga ggcgctcggt ctcttcaggc
     1801 gggctgacga agtggatcct cgccagcttg ggatggatgc tgcagaggag gtggcgcacc
     1861 gcgccaccgg tccgaaggct caccacgagc gcatcccggg agggctggcg gaccccggca
     1921 aagcgcgccc gccggcatgc ctgaatctcg cgcgctaccg ccgcgagagc gaggctgtcg
     1981 aagtgcgcct ccgtctgctc acccaccgta ggaggagatt gtgcggcggt cgccggactc
     2041 tcctgtgtgc cgttgaggag tatgaccggg tttggctccg gcgacctcac cacggtggcg
     2101 gggcggtacg tcgtcgaggc ccggtcgctc aaccaccggt tcctcgaagt cctggtccgc
     2161 ctcccccgcg atctggctcc gctggaggag cgcatccggg cccttgtgca ggggcgaatc
     2221 ttgcggggcc gggtggagat tgctattatc agggataact acggaaagcg ggtgcggacc
     2281 gccaaggtcg atgtagacct ggcaaaagcg ttcaccagcg ccctgaatga cctcaaacag
     2341 gctctggatc tgcccgggac cctggatctg tccatggtgg tcgggctgcc cgacctcatc
     2401 aaggtcgagg agcccaagga ggatctggag gagatctggc ccgccatcgc cgtggggatc
     2461 ggccaggcgc tcggccgcct cgtggccatg cgcgagggtg aaggggggcg gctggcccga
     2521 gacctggagg agcggatcga gcgggtgggg caccggatcg gcgacgtcga gcgtcgggct
     2581 ccgctggtgg tcaaggagca cgcggcccgg ctctcccgga ggattgcgga gctcctggga
     2641 tcggtccccg tggacgaggg acgcctggcg accgaggtgg ccatgttcgc cgaccggtgt
     2701 gacatcgccg aagagatcac ccggttccga agccatctgg cgcagatgcg acagaccctc
     2761 ggcgcggagg gcgccgtcgg acggaccctg gagttcatcg cccaggagtt ggggcgggaa
     2821 gtgaacacga tggggagcaa ggcgaacgac ctggagatcg cccgcgccgt gatcgcgatt
     2881 aagggcgaac tggaaagctt gcgcgagcag atccagaacg tggagtgagg agcccagcgc
     2941 cgatggagac gcggctgatc aacatcgggt tcggcaacat cgtcgcggcg aaccgcatca
     3001 tcgcgatcgt cagccccgac tccgccccga tcaagcgaat catccaggag gcccgcgaca
     3061 aggg
//
LOCUS       DASQGH010000525         7512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19279, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000525 DASQGH010000000
VERSION     DASQGH010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19279"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VEW91_10310"
     CDS             <1..217
                     /locus_tag="VEW91_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYM92013.1"
                     /translation="GAGYAMMFNNLLPIFGMLLGGAVLHERLTIVQVLASGLIIAGIA
                     IAYRSLPMTSTPASARDAVARGDSDAG"
     gene            465..2093
                     /locus_tag="VEW91_10315"
     CDS             465..2093
                     /locus_tag="VEW91_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92014.1"
                     /translation="MRDSHLQGELGQHLRQELLAGRISRREFLRSATVLGLSAGTIAA
                     LLAGPGREAVRAQAPPAKRGGTIRVALVPPTAAVDPVTMYDAGAIAVVQQVAEYLIWA
                     EKDLRLRPVLAMRWEPDSQARTWTFTLREGVKFSTGQPLTADDVVATFDRLTDPQTKS
                     AARSNFKGILSKGNVEKLAPHRVAFHLDRPFVDFPYLLSSTNYNAVVLPQTYAGDFEK
                     QPVGTGPFSLTAYTPKESASFKRNPGYWQKDLPYLEGVEFRFFAEIQPQVLALQAGAV
                     DMMLATPFQGSQAVFNDPNITVLLTSSSKHRAVHMRVDTAPFTDKRVRQALALCLDRP
                     KIVQGVFQGRADVGNDEMFAPIFPGSPTIPQRTQNYDMARQLLREAGQADLKITLTYE
                     EYQEVPQYAVFLQEMVKPAGVTVTLDQVTQAAYYGSGSNQPWLQVPMGITDWASRTVP
                     SQFILPAYTCGGVWNSAHWCNKGYDDLAEQYDATLDKQSRQSIARRLAMIQQDATPAI
                     IAYWIRAPRAVRKRVQGVEPNGSDFLDLTRASLA"
     gene            2113..3060
                     /locus_tag="VEW91_10320"
     CDS             2113..3060
                     /locus_tag="VEW91_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92015.1"
                     /translation="MTRFLLRRLGLAVPALWLVSALIFALAEVVPGDVARTILGPYAT
                     PAQVASLRHQLGADRPLVVRYAAWLGGFIMGNWGDSLVLHRPVRPFVFARLGNSLQLA
                     LVALVLVIPVSLALGILAALHEDRMLDRVISLVGLSLTAIPEFVSGVILLVILGVQLG
                     WFPIIAQPPPGVGPAGRIRYLVLPAIPLMFVLFGYIARMARAGMVDVMGSGYIRTAVL
                     KGLPPLHVIFHHALRNALLPAIAVIGSQIGWLVGGLVVVETLFTYPGLGKLIYDSAIG
                     HDVPVLEATALLVATIFMLSNLAADAVVALLQPRVRFSS"
     gene            3060..3956
                     /locus_tag="VEW91_10325"
     CDS             3060..3956
                     /locus_tag="VEW91_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92016.1"
                     /translation="MAAARWGPALPEWRTAPADAASARGAGRAAVRRLVRNPVFLVGA
                     AVVCFWILAAVEWRVLAPYDPFAIDPAHPLGPPSWAHWLGTDDLGRDVFSRVLAGAAP
                     VLVVAPTATILSLAGGTTLGLVAGYHRGLVDDVLMRLVDAVMSLPAIIPATLVLALVG
                     RSSTNVMLAIAVLFTPLVARTVRSAVLAEREREYVDAARLRGDRSLYIMFFEILPNVA
                     SPVIVEGTVRVGYAVFTAGTLSFLQLGIQPPSPDWGLTVAVERAFVQIAPWTVLAPAV
                     ALATLVVGINLAADGLRRTLAE"
     gene            3953..6013
                     /locus_tag="VEW91_10330"
     CDS             3953..6013
                     /locus_tag="VEW91_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92017.1"
                     /translation="MTTPALEVLGLTVSYRRAGEWATAVSSVSLAIGAGEAYGLVGES
                     GCGKTTLALALMRYLPRNGRIDAGAIRVDGHDLARMAERDLRRWRGTRLAMVYQDPAS
                     ALNPALRIGDQIAEVYRFHRGETPRGALRSAHEALVRVRMPDPGAVLHRYPHQLSGGQ
                     QQRVVIAMALAGNPRLLILDEPTTGLDVTIEAEVLELITALRQEIDAAILLISHNLGL
                     VARMCDRIGVMYAGQVVEEGRAREVLTTPRHPYTMGLLRCLPRFGATKQEILLEPIPG
                     GLPFLGDRIPGCAFHPRCVLARDRCRTEAPELQGVEPGRRSRCHFWAEVETMPRVTPA
                     APLRSSPVSHERLLTITGLRKTYRGRGQSTVAVHDVSLEVMKGETFGLVGESGSGKTT
                     LARCLVGLTEPDGGELTFAGRQAPWRGRDRGRATLQAMQMVFQNPDTTLNPSWTVRGI
                     LGRAVRVFLGLHGRAQARRVAELASRVRLEPRHLDLRAGQLSGGLRQRAAIARALAGK
                     PALAVCDEPVSALDVSVQAAILGLLLELQAAEGVSYVLISHDLAVVRYLADRIGVMYL
                     AQLVEVGEAAAVFGGPQHPYTEALLSAVPALDLETTVPRLRLGGPIPSLSHPPAGCRF
                     HTRCPRKLGAICEEQEPPWQDAGAGHLIRCHIPPDELRKLQTRNGRSGNGHVGDRPNP
                     SSNP"
     gene            complement(6048..6563)
                     /locus_tag="VEW91_10335"
     CDS             complement(6048..6563)
                     /locus_tag="VEW91_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYM92018.1"
                     /translation="MMARAQPRDRGGGRRGRWETPRGRPRHFGPPQAGGDEIVARLER
                     LESVVMAIAAHLGIDAGRPARPAGTPQPRGARRSEARRGDEDRAAGSGEAHAVVRTVA
                     RRLSGRVKQYEPARGYGFVVSPDTPGDVFFHRSDCRSDPATLEPSAAVTFDLVEMANG
                     QFKAVNLAPAG"
     gene            complement(6688..7311)
                     /locus_tag="VEW91_10340"
     CDS             complement(6688..7311)
                     /locus_tag="VEW91_10340"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HYM92019.1"
                     /translation="MAQALENETVFEFTGGSLCLDFANTLGGTRPRPNEHLKSYPDLI
                     AWGRQADVLTDGDARHLAQLAAQHPRDAARALAKAVAVRELLYRVLSSVIDGSRASEE
                     DFASLNAALSRALDRLRIVAHAGGFGWAWATDGDALDRMLWPVMRSAADLLVSGEVRR
                     ARRCASESCNWLFLDTSRNHSRRWCDMRSCGNRAKARRYYARKKAGR"
BASE COUNT         1077 a         2669 c         2538 g         1228 t
ORIGIN      
        1 cggcgcaggc tacgcgatga tgttcaacaa cctgctcccg atcttcggga tgctcctcgg
       61 cggggcggtc ctccacgaac ggctaacgat cgttcaggtc cttgcgtcgg ggctgatcat
      121 tgccggcatc gcgatcgcct accgatcgct gcccatgacc tccacccccg cctcggcacg
      181 ggacgccgtc gctcgggggg actccgacgc cgggtgagct tcttggggga actttgcatt
      241 gcaggaaccg cttccgtata atacgaatgc gtttcgaccg gccatgaccc tcatccgctg
      301 cagctcccat cggaccgccc accgcggtcg atcccgcctc cccgttcgtg tcggtggggt
      361 cgggaaaatc cagccgttcc tgcgttccga aggattcctc ggcagggtca aagacctgcg
      421 ccttggcctc gaggggaagt tgaaagacga ggagggggat tgctatgcgc gatagccatc
      481 tgcaaggcga actcggccag cacctccgcc aggagctgct ggccggtcgc atctcacggc
      541 gggagttcct gaggagcgcg acggtgctgg ggctgtcggc ggggacgatc gccgcgctcc
      601 tcgcgggccc cgggcgcgag gccgtccgcg cccaagcccc accggccaaa aggggcggga
      661 cgatccgggt cgcgctcgtc cccccgaccg ccgcggtcga tccggtgacg atgtatgacg
      721 cgggcgcgat cgcggtcgtc cagcaggtgg ccgagtacct gatctgggcg gagaaagacc
      781 tcaggctgcg ccccgtgctg gcgatgcggt gggaaccgga cagccaggcc cggacgtgga
      841 cgtttacgct tcgggagggg gtcaaatttt ccacggggca gccgctgacc gcggacgacg
      901 tggtggccac gttcgaccgg ttgaccgacc ctcagacgaa atccgcggcc cgctcgaatt
      961 tcaagggcat tctctcgaag ggcaacgtcg agaagctcgc gccgcaccgg gtggcatttc
     1021 acctggaccg gccctttgtg gacttcccct acctcctgtc ctccacgaac tacaacgccg
     1081 tcgtgttgcc gcagacctac gccggcgatt tcgaaaagca gcccgtaggc acagggccgt
     1141 tctcgctcac cgcctacacg cccaaggaga gcgcttcgtt caagcggaac ccgggatact
     1201 ggcagaagga tcttccctac ctcgagggcg tggagtttcg cttcttcgcg gagatccaac
     1261 cccaggtctt ggcgctgcag gccggggcgg tcgacatgat gctggcgacc ccgtttcagg
     1321 gatcgcaggc ggtgttcaat gacccgaaca tcaccgtgct cttgacctcg tcgtccaagc
     1381 atcgggcggt gcacatgcgg gtggataccg cgcccttcac agacaagcgc gtgcgccagg
     1441 cgctggcctt gtgcctcgac cggccgaaga tcgtgcaggg cgtcttccag ggccgggccg
     1501 acgtggggaa cgacgagatg ttcgcgccga tctttccggg ctcgccgacg atcccccagc
     1561 gcacccagaa ctacgatatg gccaggcagc tgctccgcga agcggggcag gcggacctca
     1621 agatcacgct cacgtacgag gagtaccagg aggttccgca gtacgcggtg ttcctgcagg
     1681 agatggtgaa accggccggc gtgaccgtca cactcgatca ggtcacccag gccgcgtact
     1741 acggatccgg aagcaaccag ccgtggcttc aggtccccat gggcatcacc gactgggcgt
     1801 cccggaccgt tccgtcccag ttcatcctgc cggcgtacac ctgcgggggc gtgtggaact
     1861 ccgcgcactg gtgcaacaag gggtacgacg acctcgccga gcagtacgac gccaccctgg
     1921 acaagcagag ccgccagtcg atcgcgcgca ggctggccat gatccagcag gacgcgaccc
     1981 ctgcgatcat cgcctactgg atccgggcgc cccgcgcggt gcggaagcgg gtccaggggg
     2041 tcgagcccaa tggatcggac ttcctggatc tgaccagggc ctctctggcc tagaccggca
     2101 cggccgaagg gcatgacgcg cttcctcctc aggaggttgg ggctcgcggt gcccgccctg
     2161 tggctcgtct ccgcgctgat cttcgcgctc gcggaggtcg tgccggggga cgtggcccgc
     2221 accatcctgg ggccgtacgc gaccccggcc caggtcgcat cgctcaggca ccagctcggt
     2281 gccgaccgcc cccttgtggt tcgttacgcg gcgtggctgg ggggcttcat catgggcaac
     2341 tggggggact cgctggtgct ccaccggccc gtccgcccgt tcgtgttcgc gcgccttggg
     2401 aactccctcc agctcgccct ggtcgcgctc gtcctggtca ttccggtctc ccttgccctg
     2461 gggatcctcg ccgccctgca cgaggaccgc atgctcgacc gggtgatcag tctcgtgggc
     2521 ctctcgttga ccgcgatccc tgaatttgtg agcggggtga tcctgctcgt gatcctgggc
     2581 gtccagctgg gctggttccc gatcatcgcg caacccccgc caggggtggg ccccgccggg
     2641 cgcatccgct atctggtcct tccggcgatc cccctcatgt ttgtgctgtt tggctacatc
     2701 gcccggatgg cgcgggcggg gatggtcgac gtcatgggat cgggctacat caggaccgcc
     2761 gtgctcaagg gcctgccccc actgcacgtg atcttccacc acgcgctccg caacgcgctc
     2821 ttgccggcga tcgcggtcat cggctcgcag atcggatggc tggtgggagg gctggtcgtg
     2881 gtggagaccc tgttcaccta tcccggattg ggcaagctca tctatgactc ggcgatcggg
     2941 cacgacgtgc ccgtgctcga agcgacggcg ctcctggtgg cgaccatctt catgctctcg
     3001 aatcttgccg cggacgcggt ggtggcgctc ctccagccgc gggtccggtt ctcgtcctga
     3061 tggcggccgc gcgttgggga cccgcgctgc cggagtggcg caccgcaccc gccgatgcgg
     3121 cgagcgcgcg aggcgccggg agagccgccg tccgccggct ggtccgaaac cccgtgtttc
     3181 ttgtcggcgc cgcggtcgtg tgcttctgga tcctggcggc cgtggagtgg cgggtgcttg
     3241 cgccgtacga cccgtttgcg atcgaccccg cgcaccccct cggcccgccc agctgggcgc
     3301 actggctcgg cacggacgat ctcggccgcg atgtgttctc ccgcgtcctg gccggggcgg
     3361 cgccggtcct cgtcgtcgcg cccacggcca cgatcctgag cctcgccggc gggacgacgc
     3421 tgggcctggt ggcggggtac catcggggtc tcgtcgatga cgtcctgatg cggctcgtcg
     3481 atgccgtgat gagcctgccg gcgatcatcc cggccacgct ggtcctggcg ctcgtggggc
     3541 ggtcctcgac caacgtgatg ctcgccatcg cggtgttgtt caccccgctg gtcgcccgga
     3601 cggtgcggtc ggccgtgctg gccgagcggg agcgcgaata cgtcgacgcg gcgcgcctgc
     3661 ggggcgaccg gagcctgtac atcatgttct tcgagatcct ccccaacgtg gcctcgccgg
     3721 tcatcgtcga aggaacggtc cgggtggggt acgcggtgtt caccgcaggg acgctctcct
     3781 tcctccagct ggggatccag ccgccgtccc cggactgggg gctcacggtc gccgtggagc
     3841 gcgcgttcgt gcagatcgcc ccgtggaccg tgctcgcgcc cgcggtcgcc ctcgcgacgc
     3901 tggtcgtcgg catcaacctc gcggccgatg ggctgcggag gaccctggcc gaatgaccac
     3961 cccggcgctc gaggttctgg ggcttacggt ctcctaccgc cgggcgggcg agtgggcgac
     4021 cgcggtctcc tccgtctcgc tcgcgatcgg cgccggggaa gcgtacgggc tcgtcggcga
     4081 gtccgggtgc gggaagacca cgctggccct ggcgctcatg cgctacctgc cgcggaatgg
     4141 ccgcatcgac gccggcgcga tccgcgtgga tgggcacgac ctggcgcgga tggcggaacg
     4201 cgatctccgc cggtggcgcg gcacccgcct ggcgatggtc taccaggatc ccgccagcgc
     4261 cctgaacccc gcgctgcgga tcggggacca gatcgccgag gtctaccggt tccatcgggg
     4321 cgagaccccc cgcggggcgc tccgctccgc ccacgaggcg ctcgtgaggg tacggatgcc
     4381 cgaccccggc gcggtgctgc accgctatcc ccaccagctc tcgggggggc agcagcagcg
     4441 cgtggtaatc gccatggcgc tcgcgggcaa cccgcggctc ctgatcctcg atgagcccac
     4501 cacggggctc gacgtcacca tcgaagcgga ggtcctcgag ctgatcaccg cgctccgcca
     4561 ggagatcgac gcggcgatcc tgctcatcag ccacaacctc gggctcgtcg cccggatgtg
     4621 cgaccggatc ggggtcatgt acgcagggca ggtcgtcgag gagggccgcg cgcgggaggt
     4681 gctcacgacc ccgcgccacc cctacacgat ggggctgctc cgctgcctgc cgcgcttcgg
     4741 agccacgaag caggagatcc tcctcgagcc gatcccgggg gggcttccgt tcctggggga
     4801 ccgcattccc gggtgcgcct ttcacccccg ctgcgtcctg gcccgggacc ggtgccggac
     4861 ggaagcgccg gagctgcagg gcgtggagcc ggggcggcgg agccgctgcc acttttgggc
     4921 ggaggtcgag accatgccgc gcgtcacccc ggccgccccc ctccggtcca gccccgtttc
     4981 gcacgagcgg ctcctcacga tcacggggct ccggaagacc taccgcggac gcggccagag
     5041 caccgttgcc gtgcacgatg tctcgctcga ggtgatgaag ggggaaacct ttggcctggt
     5101 cggcgagtcg gggagcggca agaccacgct ggcgcggtgt ctggtgggac tgaccgagcc
     5161 cgatggcggc gagctcacct ttgccggcag acaggcgccg tggcggggcc gggaccgggg
     5221 gcgcgccaca ttgcaggcga tgcagatggt gttccaaaac cccgacacca cgctcaaccc
     5281 gagttggacg gtccgcggaa ttctcgggcg cgcggttcgg gtgttcctgg gtctccacgg
     5341 ccgtgcgcag gcccgccgcg tcgccgaact cgcatcgcgg gtgcgcctcg agccgcggca
     5401 cctggacctc cgcgccggcc agctctccgg tggcctcaga cagcgggcgg cgattgcgcg
     5461 ggcgctggcc gggaaaccgg cgctcgcggt ctgcgacgag cccgtctccg cgctcgacgt
     5521 ctcggtccag gcggcgatcc tcggcctgct gttggagctg caggccgctg agggcgtctc
     5581 gtatgtcctt atctctcacg acctcgccgt ggtccgctac ctggccgacc gaatcggcgt
     5641 catgtacctg gcgcagctcg tggaggtcgg ggaggccgcg gccgtgttcg gcggccccca
     5701 gcatccttac accgaggcgt tgctctcagc cgtcccggcc ctcgatctgg agaccaccgt
     5761 gccgcgcctc cggctgggcg ggcccatccc aagcctatcc catccgccgg ccgggtgccg
     5821 gttccacacc cggtgcccga ggaaactcgg cgcgatctgc gaggagcagg agcctccctg
     5881 gcaggatgcc ggcgcggggc acctcatccg gtgccacatt cctccagacg aactccggaa
     5941 gctgcagacg cgaaacggac ggtctggaaa cgggcacgtt ggggaccggc ccaacccgtc
     6001 atcgaatccc tgatcggact gagccctgcg gctgtcttcc ggtggggtca ccccgccggg
     6061 gccaggttca ccgccttgaa ttgaccgttg gccatctcca cgagatcgaa ggtgaccgcg
     6121 gccgacggct cgagcgttgc gggatcggac cggcagtcgc tccggtggaa gaacacgtcc
     6181 cccggtgtgt ccgggctgac gacgaacccg tacccacgcg ccggttcgta ctgcttgaca
     6241 cggccggaca gtctgcgcgc gacggtgcgg acaaccgcgt gggcctcacc ggacccggcc
     6301 gcgcgatcct cgtctccccg tcgcgcttcg cttcgccgcg cacccctcgg ctgcggggta
     6361 cccgccggcc gcgcggggcg gccggcatcg atgcccaggt gggcggcgat cgccatcacc
     6421 acggactcga ggcgctcgag ccgcgcgaca atctcgtccc cgcccgcctg cggcgggccg
     6481 aaatgcctgg gccgccccct tggcgtctcc caccggcctc gccgcccgcc tccccgatcg
     6541 cgtggctgcg ccctcgccat catgctccct ccaggaaagc ccgaatgccc gcgataccct
     6601 tctccggcgt ccctggcctc atccttcgct cggagcgaag gctggtcgtt cggatcgcga
     6661 cacccagcgc cgcggcggcg ggcaccccta gcggcccgcc ttcttgcgcg cgtagtatcg
     6721 ccgcgccttt gcgcggtttc cgcacgaccg catatcgcac cagcgacggc tgtggttccg
     6781 gctggtgtcc agaaacagcc agttgcagct ttcgctcgcg cacctccgag ctcgccgtac
     6841 ctccccggac accagcaggt ccgccgccga gcgcataacg ggccagagca tccgatccaa
     6901 ggcatccccg tcggtcgccc atgcccagcc gaacccgccg gcgtgtgcga ctatccgcag
     6961 ccggtccagg gcgcgggaca gggcggcgtt cagggacgcg aaatcctcct cggaggcccg
     7021 cgagccatcg atcaccgagg aaagaacgcg gtagagcaat tcccggaccg ccaccgcctt
     7081 ggcgagcgcc ctggccgcat cccggggatg ctgcgccgcg agctgcgcga ggtgacgggc
     7141 gtctccgtcg gtcagcacgt ccgcctgccg cccccacgcg atcagatccg gataactctt
     7201 cagatgttca ttggggcggg ggcgggtccc gccaagcgtg ttggcaaaat ccaggcacag
     7261 actcccgcct gtgaactcga agaccgtctc attctccaac gcctgcgcca tggtccctcc
     7321 gcccaagtcc cggaaaggga gcccgggctc cgctcaaacc gtgcataacc aacaaaaagt
     7381 gcttgacagg ttagacacgg tttgttactc tataaccaac aaaacaatag taacaggtta
     7441 tatcggttct ggcaagcgcc ggccgtgcaa cccgcgccga ggattcgggt taaggaagtg
     7501 tagaccggtc cg
//
LOCUS       DASQGH010000526         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27717, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000526 DASQGH010000000
VERSION     DASQGH010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27717"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VEW91_10345"
     CDS             complement(<1..348)
                     /locus_tag="VEW91_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYM92020.1"
                     /translation="MLHHLTEKREKNVVRPRQRASSGSPYEPKFGISRAVRAGRNIAV
                     TGTAPLGPDGRTVGHGDAAAQARRCFEIICAALQEVGADLADVIRTRTILTRIEDYEK
                     VMLVHGEFFRDIRP"
     gene            complement(410..976)
                     /gene="pyrR"
                     /locus_tag="VEW91_10350"
     CDS             complement(410..976)
                     /gene="pyrR"
                     /locus_tag="VEW91_10350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HYM92021.1"
                     /translation="MELREKAKVMDRETIRRTLTRMAHEIVERNAGGAGLCLVGICRR
                     GVPLAERLAAYIAQAEQVQIPVGTLDVTRYRDDLPARERAGNGGAEPGMETRLPFPVA
                     NRRIILVDDVLYTGRTVRAGLDALIDRGRPACIQLAVLIDRGHRELPIRPDFVGKNLP
                     TSSREIVTVRVAEVDGEDEVVIEEPAEP"
     gene            complement(982..>1918)
                     /locus_tag="VEW91_10355"
     CDS             complement(982..>1918)
                     /locus_tag="VEW91_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYM92022.1"
                     /translation="PPAADRGRRLDVVVAGRFPQYSRSRVATLAGRGQVLVDGRSQKP
                     AFRLRAGQRVRVLAPPSDPVVVAPQPIPLQIVHEDADILVIDKPAGMTVHPAPGHPNG
                     TLVNAVLAHVPDLSGIAGPLRPGVVHRLDKDTSGLLVIAKTDAAYRSLVAQLRARAVT
                     RVYLALVRGTVREDRGVISAPIGRHPVHRTRMAVVPHGRPAVTHVTVRERLEGATLVE
                     CRLETGRTHQIRVHLRHIGHPVLGDPVYGGGDGGIGRQALHAARLEFTHPRTGERIVC
                     TAPLPEDFARALDTLRRAGSPGAPSGRARSGKREV"
BASE COUNT          266 a          688 c          675 g          289 t
ORIGIN      
        1 cggtctgata tctcgaaaga actcgccgtg caccagcatt accttttcat agtcttcgat
       61 tcgggtcagg atggtccgcg tccggatcac atccgcgaga tccgcgccca cttcctgaag
      121 cgccgcacag ataatctcga agcatctgcg ggcttgggct gccgcatcgc cgtgccccac
      181 cgtgcgcccg tccggtccca atggcgcggt gccagtgacg gcgatgttgc ggcccgcgcg
      241 gacggcacgc gaaatgccga acttcggttc atatggagac ccggatgacg ctcgctggcg
      301 aggacgcacc acgttcttct ccctcttctc tgtcaggtgg tgaagcactc ttatcggact
      361 ggcgctcccg gcatcagacg agccttaccc ggatttggtg acgcgcgcgt cagggctccg
      421 ccggctcctc gatcaccacc tcgtcttcgc cgtccacctc agcgacccgg acggtcacga
      481 tctcccgcga cgaggtcggc aggttcttgc cgacaaagtc gggccggatc ggcagttcgc
      541 ggtgcccgcg gtcgatcagc acggcgagct ggatacacgc gggccggccg cggtcgatca
      601 gggcgtcgag cccagcccgg acggtccgcc cggtgtagag cacgtcgtcg accaggatga
      661 tccgccggtt ggccacgggg aacggcagcc gggtttccat cccgggttcg gctccgccgt
      721 tccccgcgcg ctctcgcgcg gggagatcgt cccggtagcg ggtcacgtcc agggtaccga
      781 ccgggatctg cacctgctcc gcctgcgcga tgtacgcggc cagccgctcg gcgagcggca
      841 cgccccgccg gcagatgccg accaggcaca ggcccgctcc gccggcgttg cgctcgacga
      901 tctcgtgcgc catccgcgtg agggtgcggc ggatcgtctc gcgatccatc accttggctt
      961 tctcccgcag ctccacgggg cctacacttc gcgctttccg gaccgcgccc tgccggaggg
     1021 ggctcccgga gatcccgccc ggcgcagcgt gtccagcgcc cgcgcaaaat cctcggggag
     1081 gggcgccgta cagacgatgc gttccccggt gcgggggtgg gtgaactcca gccgcgcggc
     1141 gtggagcgcc tggcgcccga tccccccatc cccgccgccg tagacgggat ccccgaggac
     1201 cgggtgcccg atgtggcgca ggtggacgcg gatctggtgg gtccggccgg tctccagccg
     1261 acactccacg agcgtcgccc cctccagccg ctcccggacg gtcacgtggg tgaccgccgg
     1321 acgcccgtgc gggacgaccg ccatgcgggt gcggtggacg ggatgccgcc cgatcggcgc
     1381 tgagatcacc ccgcggtcct cgcggacggt tccgcgcacg agcgccaggt acacccgggt
     1441 gacggcgcgc gcgcggagtt gcgccacgag cgaccggtac gccgcatcgg tcttggcgat
     1501 gaccaggagg cccgaggtat ctttgtccag ccggtgcacg acccccgggc ggagcggacc
     1561 cgcgatcccc gagagatcag gaacgtgggc cagcaccgcg ttcacgagcg tgccgttggg
     1621 gtgcccgggt gcggggtgca cggtcatgcc tgccggcttg tcgatgacga ggatgtcggc
     1681 gtcctcgtgc acgatctgga gcgggatggg ctggggagcc acgaccaccg ggtccgacgg
     1741 cggtgccaag acccggaccc gttgtccggc ccggagccgg aacgcgggct tctgcgaccg
     1801 gccgtcgacc agcacctgcc cgcgcccggc gagggtggcc acgcgcgacc gcgagtattg
     1861 tgggaagcgc ccggcgacga cgacatcgag gcggcggccg cggtcggcgg ccggcggc
//
LOCUS       DASQGH010000527         6720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36292, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000527 DASQGH010000000
VERSION     DASQGH010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36292"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VEW91_10360"
     CDS             complement(<1..1794)
                     /locus_tag="VEW91_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYM92023.1"
                     /translation="MRRPTLIWLVTWGPAIAGTAAIAAFGRGVAFPPAAVLLIVTALA
                     AAAEHIHIPIPSGGYQPFGPAVSLPAIVLLGPIPAAWATAAGTIISDGILRRRQIATM
                     LFTVGQRSLTILLAGLVWNTLLRGFPSSDRVSLIAHEESILTAVLALLAVYAVATTIQ
                     VAAFLSVFRQEPFWEVLASGAKWQLPTTVILGGFGLSVSVLFSEIRTQTVGEVGYLLT
                     ALLAGSLIALLYTSRRQQASEFTSLHTAVADLLQTLDLGEVLNLLADKVTRIAKPDML
                     WITLQRPDGGYEVALARTQAVDRDLLTPTPGELEWGVLGWVLANRRSQRISDYRRDPR
                     RTPESSAIFPPDHARAVLMAPLLAGIEPVGAITLTKAIPDYFTEYQERVVSTLAAQTA
                     VVVRNAQLYETSQRTLAHFEALKAISERINSQQDLQAAFDLIATSAGEVLGADRCALY
                     LGEMGKEITHTFTRGLPAEHLRSAVARLQSGEGLLSMAIRQKEPMVATDALVDARVSR
                     EMAAGAGYRTVAGFPLRYREAIIGVLALYHNEVHPYGASETALGMAFANQAAMAVQNT
                     RLLGEAGHRAHQLGLLNRIFTRVATSLSPQDL"
     gene            complement(1884..2132)
                     /locus_tag="VEW91_10365"
     CDS             complement(1884..2132)
                     /locus_tag="VEW91_10365"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HYM92024.1"
                     /translation="MPVLKLYLGDVVRTRKTHPCGGDQWEIVRLGADIGIRCLRCGRR
                     VLIPRPKLERRIREFVRRGPGAGGAPDAEAPPSPAAEV"
     gene            complement(2154..3611)
                     /locus_tag="VEW91_10370"
     CDS             complement(2154..3611)
                     /locus_tag="VEW91_10370"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYM92025.1"
                     /translation="MLLALEIVASRVIAPYFGNSVYVWGSLIGVFLAALSVGYFLGGR
                     ISTRWPHPGPFLALVFLAGAATYPIPHFADAVLGQIASRDLGPRGNPLAGSAALFFVP
                     SALMATISPYAVRLRARSVEGVGDVAGMLYALGTLGSIVGTLLAAFVLISTFGVRSII
                     QILGGTEMALAVAGLLWTRRAGAAAGAAAGMAAVLSLTGSGPADAADVIHARDTIYHR
                     ITVSDEGQVRYLRLDNYWQSARDRSDPLRTVFAYTDYLHLPVLFVPRLHRVLFVGMGG
                     GTAPGRFHHDYPQATIEVVEIDPEVVATARRLFALPQDARLQVHVMDGRLWLRRSTER
                     YDLVILDAYLIDTIPFHLATREFYQEMAARLTPGGAAASNVIGAVRGPQSRLFRAIYK
                     TFRSVFPRVYVFPVGGSEPESLRNIILVGTGDPPLTHSEILARAAATEQSGRIRIEGF
                     SRDAGTLLDGPIETGDVPILTDDYAPTDALIAPAR"
     gene            complement(3632..6274)
                     /gene="clpB"
                     /locus_tag="VEW91_10375"
     CDS             complement(3632..6274)
                     /gene="clpB"
                     /locus_tag="VEW91_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HYM92026.1"
                     /translation="MARRFDRFTEKAQEALITAQAVAKEHNHSQVDGEHLLQALLSQS
                     DGIPQALLGRMGIGVGPLRAQVEQALARQPKVYGPEEPAMGPGLRRTLEGATAEADRL
                     KDEYVSTEHLLLGLAADRNTTAGRLLAASGVDREKVYAALQAIRGGQRVTDTSPETKY
                     QALERYGRDLTKMAREGKLDPVIGRDDEIRRIIQVLSRRTKNNPVLIGDPGVGKTAIV
                     EGLAQRIVRGDVPEGLRNKRVVALDLGALIAGTKFRGEFEDRLKAVLRDITEAAGEVI
                     LFIDELHTVVGAGAAEGAMDASNMLKPMLARGELHAIGATTLDEYRKHIEKDPALERR
                     FQPVFTDEPSVEETISILRGLKEKYEVHHGVRITDSAVIAAATLSDRYISDRFLPDKA
                     IDLIDEAASRLRMEIDSKPAPLDEADRRIMQLEIEREALRKETDPASQERLQRIEREI
                     ADLREQSRALRARWDQEKALIGDLRAIKAQIDETRVEIEKAERAADLERAARLRYGTL
                     RELNERRAAQEARLNSLGEGRLLKEEVDAEDVAAVVSHWTGIPVTRLMEGEMQKLLNL
                     EDRLHERLVDQQDAVRAVADAIRRSRAGLADPKRPVGSFLFLGPTGVGKTELARALAM
                     LLFDDEDAMVRLDMSEYMEKHTVSRLIGAPPGYVGYEEGGQLTEAVRRRPYRVILFDE
                     IEKAHPDVFNIFLQILEDGRLTDGHGRTVDFRHAIIIMTSNMGSQYLRNLDPGDQPGF
                     ELVRVQIMDELRRKLRPEFLNRVDEIIVFRPLSRTDLERIVDLQLAGLSARLAARHLH
                     LEVTKEAKAVLAQEGYNPDFGARPLRRLIQRTVETAVSRQLLAGEFREGDTVVVEAKG
                     GEIAVRRGAQAPAPSRGGSRASSN"
     gene            complement(6293..>6720)
                     /locus_tag="VEW91_10380"
     CDS             complement(6293..>6720)
                     /locus_tag="VEW91_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYM92027.1"
                     /translation="KTRGLEVTIPPGVRHGQRIRAAGQGQGGDLYLTVQIAPHPLFTR
                     VEDDVHYELAVPVWVAALGGTVEVPTLGGRVTMTIPAETQDGRTFRLRGRGLPHLRAG
                     GAGDELVKLRLALPQPLTPRDRELFEEIRRLHGARAAKS"
BASE COUNT          980 a         2308 c         2369 g         1063 t
ORIGIN      
        1 gaggtcttgc gggctgagcg acgtggcgac ccgcgtgaag atgcggttca agaggccaag
       61 ctgatgggcc cggtgccccg cctccccgag gagccgcgtg ttctgcacgg ccatcgccgc
      121 ctggttggcg aaggccattc ccagcgcggt ctccgacgct ccgtaagggt gcacctcgtt
      181 gtggtagagc gccaggaccc cgatgatggc ctcgcggtac cggaggggga agccggcgac
      241 ggtccggtat cccgcgccgg cggccatctc gcggctcacc cgcgcatcca cgagggcgtc
      301 ggtggcgacc atcggttctt tctgacggat cgccatgctg aggaggccct ccccgctttg
      361 gagcctcgcc acggcggaac gcagatgctc ggcggggagc ccgcgggtga aggtatgcgt
      421 gatctccttc cccatctccc cgaggtagag ggcgcatcga tccgccccga ggacctcacc
      481 ggcgctcgtc gcgatgaggt cgaatgccgc ctggagatcc tgctgagagt tgatgcgctc
      541 ggagatcgcc ttcagggcct cgaagtgggc gagggtccgc tgggacgtct cgtagagctg
      601 ggcgttgcgc accaccacgg cggtctgcgc ggcgagggtg ctgacgaccc gttcctggta
      661 ctcggtgaag tagtccggaa tggctttggt gagcgtgatc gcccccaccg gctcgatgcc
      721 cgccagcagc ggggccatga gcacggcccg cgcatggtcg gggggaaaga tcgcgctgct
      781 ctccggcgtc cgccgtggat cccgccggta atcggagatc cgctgggatc ggcggttggc
      841 cagcacccag ccgagcacgc cccactcgag ttcgccgggc gtgggcgtga ggaggtcgcg
      901 atcgaccgcc tgcgtcctgg ccagggcgac ttcgtacccc ccatcggggc gttggagcgt
      961 gatccacagc atgtccggtt ttgcgatgcg cgtcaccttg tccgcgagga ggttgagcac
     1021 ctccccgaga tcaagggttt ggagcagatc ggccacggcg gtgtgcaggc tggtgaattc
     1081 actggcctgc tgccgtcgcg aggtgtagag gagcgcgatc aggctccccg ccaagagcgc
     1141 ggtgagcaga tacccgacct cgcccaccgt ctgggtgcgg atctcgctga acagcacgga
     1201 gaccgagagg ccgaacccgc cgagaatcac cgtcgtgggg agctgccact tcgcgccgga
     1261 ggccagcacc tcccaaaacg gctcctggcg gaacacggag aggaaggccg cgacctggat
     1321 tgtcgtcgcg accgcgtaga cggcgagcag ggcgaggacg gccgtgagga tgctttcctc
     1381 gtgggcgatg aggctgacac gatcggacga cgggaagccg cgcaacagcg tgttccagac
     1441 gaggcccgcg agcaggatgg tcagggagcg ctgccccacg gtgaagagca tcgtcgcgat
     1501 ctgccggcgg cgcaggatgc catcgctgat gatcgtgcct gcggcggtcg cccacgcggc
     1561 ggggatcggc ccgaggagca cgatcgcggg aagcgacacc gccggcccga agggctggta
     1621 gccaccgctt gggatgggaa tgtgaatgtg ctccgcggcg gcggcgagcg ccgtgacgat
     1681 gagcaacacg gccgccggtg ggaaggcgac gccgcgcccg aacgcggcga tggctgcggt
     1741 cccggcgatc gcagggcccc aggtgacgag ccagatcagg gtaggccgac gcatggttac
     1801 tcggtgagcc tctcggggtg ggcttgacag ctgggcccgg cacggcccct atgttcaatt
     1861 aaatgccctt gaggaagttg ggtctagacc tcagcggcag gggaaggggg cgcctctgcg
     1921 tccggcgcgc cgcccgcgcc gggacccctg cggacaaatt cccggattct tcgctccaac
     1981 ttcggtcgag gtatcagcac ccggcggccg cagcgcaggc accggatccc gatgtccgct
     2041 cccaggcgca caatttccca ctggtcccca ccgcacgggt gggtcttgcg ggtcctgacg
     2101 acatcaccca gatagagctt cagtaccggc actgcagggc ccccgctcga cgcttaccgg
     2161 gccggcgcga tcagcgcgtc cgtcggcgcg tagtcgtcgg tcaggatggg aacatcgccg
     2221 gtctcgatcg ggccatccag gagggtgccc gcgtcccgcg agaacccctc gatccggatc
     2281 cgtccgctct gctccgtcgc ggccgcccgc gccagaatct cggagtgggt aagcggggga
     2341 tcccccgtgc caaccaggat gatgttgcgg agcgattccg gctcgctccc gcccacggga
     2401 aacacgtaga cgcgggggaa taccgaccga aaggtcttgt agatggccct gaacaaccgg
     2461 ctctgcggcc cgcggaccgc cccgatgacg ttgctcgccg cggcccctcc tggggtcagc
     2521 cgcgcggcca tctcctggta gaactcgcgg gtggccaggt ggaacgggat cgtgtcgatg
     2581 aggtacgcgt ccaggatgac gaggtcgtac cgttccgttg accggcgcag ccagagccgg
     2641 ccgtccatca cgtgcacctg gaggcgggcg tcctggggca gcgcgaagag ccgccgggcg
     2701 gtcgccacga cctccggatc gatctccacc acctcgatcg tcgcctgggg gtagtcgtgg
     2761 tggaatcggc cgggcgcagt cccgccgccc atcccgacga agagcacgcg gtggaggcgc
     2821 ggaacgaaga ggaccgggag gtggaggtag tcggtgtagg cgaacacggt gcgaagcggg
     2881 tcggagcgat cgcgggcgct ctgccagtag ttgtccaggc gaaggtaccg cacctgcccc
     2941 tcgtcgctga ccgtgatgcg gtggtagatg gtatcccggg cgtggatcac gtccgcggcg
     3001 tcggccgggc cgctgccggt gaggctcaac accgccgcca tccccgcagc cgctcctgcg
     3061 gccgcgccgg cccggcgggt ccacagcagc cccgccaccg cgagggccat ctcggtccct
     3121 ccgaggatct ggatgatcga ccgcacccca aaggtggaga tcaggacgaa cgcggccagc
     3181 agggtgccga cgatgctgcc gagcgtgccc agggcgtaca gcatgccggc gacgtctccg
     3241 acgccctcga cggaccgggc ccgcagccgg accgcgtacg gcgagatcgt ggccatgagc
     3301 gcgctgggga cgaagaacag ggccgcgctg ccggccagcg ggttcccccg gggccccaag
     3361 tcccgggacg cgatctgtcc gaggaccgcg tccgcaaagt gcgggatcgg ataggtggcg
     3421 gcgccggcca ggaagacgag cgccaggaac gggccggggt gcggccaccg cgtgctgatc
     3481 cgccctccga ggaagtaccc cacgctcagc gcggccagga agacgccgat gaggctcccc
     3541 cagacgtaga cggagttccc gaagtagggc gcgatcaccc ggctcgcgac gatctcgagc
     3601 gcgagcagca cgccaccgct cgtgaaaacg atcagttcga ggatgcccgg gatcctcccc
     3661 gcgatggggc gggcgcctgc gcgccgcggc gcaccgcgat ctcgccgccc ttggcctcca
     3721 cgacgacggt gtcgccttca cgaaactcgc cggcgaggag ctggcggctc acggcggtct
     3781 cgacggtgcg ctggatcagc cgccggagcg gcctggctcc aaagtcgggg ttgtacccct
     3841 cctgcgcgag gaccgccttc gcctccttcg tcacctccag atggaggtgg cgcgccgcca
     3901 gccgggcgga gagtcccgcc agctggagat cgacgatccg ctcgaggtcg gtccggctga
     3961 ggggccggaa gacgatgatc tcgtcgacgc ggttgagaaa ctcgggccgg agcttccggc
     4021 ggagctcatc catgatctgg acgcgcacca gctcgaaccc cggctgatca ccgggatcca
     4081 ggttgcgcag atactggctc cccatgttgc tcgtcatgat gatgatcgca tggcggaagt
     4141 ccaccgtccg gccgtgcccg tcggtgagcc ggccgtcttc gagaatctgg aggaagatgt
     4201 tgaagacgtc cgggtgcgcc ttttcgatct catcgaacag gatcacgcgg tacgggcgcc
     4261 ggcggacggc ctcggtcagc tggcctcctt cctcgtagcc gacgtagccc ggcggcgcgc
     4321 cgatcaggcg gctcacggtg tgcttttcca tgtactcgga catgtcgagg cgcaccatcg
     4381 cgtcttcgtc gtcgaagagc agcatggcga gggcccgggc tagctcggtc ttgcccaccc
     4441 cggtcggccc gaggaacagg aacgagccca ccgggcgctt cgggtcggcc aggcccgcgc
     4501 gcgaccggcg gatcgcatcg gccacggccc gcaccgcatc ctgctggtcg acgaggcgct
     4561 cgtgcaggcg gtcctccagg ttgaggagct tctgcatctc cccttccatg agccgggtga
     4621 ccggaatgcc ggtccagtgg ctcacgaccg cggcgacgtc ctccgcatcg acttcctcct
     4681 tgaggaggcg cccctcgcca aggctgttga ggcgggcctc ctgggcggcc cgccgctcgt
     4741 tgagctcacg cagcgtgccg tagcgcagcc gtgcggcgcg ttcgagatcc gcggcgcgtt
     4801 cggccttctc gatttccacc cgggtctcgt cgatctgcgc cttgatggcg cgcaggtccc
     4861 cgatcagggc cttctcctgg tcccaccgcg cccgcagcgc ccggctctgc tcccggaggt
     4921 ccgcgatctc gcgctcgatc cgctggaggc gctcctggga ggcggggtcg gtctccttgc
     4981 ggagcgcctc gcgctcgatc tcgagctgca tgatgcggcg atccgcctcg tcgagcgggg
     5041 cgggcttgct gtcgatctcc atccggagtc ggctcgccgc ttcatcgatc aggtcgatcg
     5101 ccttgtccgg caagaaccgg tcgctgatgt accggtccga gagcgtcgcc gcggcaatca
     5161 ccgcgctgtc ggtgatccgg accccgtggt ggacctcgta cttctccttg aggccgcgga
     5221 ggatggagat cgtttcctcc acgctcggct cgtccgtgaa caccggctgg aaccggcgct
     5281 cgagggccgg atccttctcg atgtgcttcc ggtactcatc gagcgtcgtc gcgccgatcg
     5341 cgtggagctc gccgcgggcc agcatcggct tgagcatgtt gctggcatcc atcgcccctt
     5401 cggccgcgcc ggcgccgacc accgtgtgca gctcgtcgat gaacaggatg acctctccgg
     5461 cggcctcggt gatatcgcgc aggaccgcct tgaggcggtc ctcgaactcg ccgcggaact
     5521 tggtgccggc gatcagcgcg ccgagatcca gggcgaccac ccgtttgttc cggagccctt
     5581 cggggacgtc tccgcgcacg atccgctggg cgagtccttc cacgatggcg gtcttgccca
     5641 cgccggggtc gccgatgagc acggggttgt tcttggttcg ccgggacagg acctggatga
     5701 tccggcggat ctcgtcgtcc cggccgatca ccgggtcgag cttcccctcg cgggccatct
     5761 tggtgaggtc gcggccgtag cgctccagcg cctgatactt cgtctcgggc gaggtgtcgg
     5821 tgacccgctg gcctccccgg atggcctgga gcgccgcata gaccttctcc cgatcgacgc
     5881 cggaggcggc caggaggcgg ccggccgtgg tgttccggtc cgccgccagt cccagcagga
     5941 ggtgctccgt gctgacgtac tcgtccttga ggcggtccgc ctcggccgtg gccccctcga
     6001 gggtgcggcg cagccccggc cccatcgccg gctcttccgg tccgtagacc ttcggctgcc
     6061 gcgccagcgc ctgctcgacc tgggcgcgca gcggccccac cccgatcccc atccggccga
     6121 gaagtgcctg gggaatcccg tcagattggc tgaggagtgc ctggagcagg tgctcgccgt
     6181 ccacctggct gtggttgtgc tccttggcca ccgcctgcgc ggtgatcaac gcctcctgtg
     6241 ctttctcggt aaacctgtcg aatcgcctcg ccatcgacgt tcgccccggg gcttaggatt
     6301 ttgccgcccg cgccccgtgg aggcggcgga tttcttcgaa cagctcgcga tcccgcgggg
     6361 tcagcggctg cgggagcgcg agccggagct tgaccagctc gtcgcctgcg cccccggccc
     6421 gcaggtgggg gaggccccgg ccgcgcaggc gaaacgtccg gccgtcctgg gtctcagcag
     6481 ggatggtcat cgtgacccgc ccgccgagcg tcggcacctc gaccgtcccg cccagcgcgg
     6541 cgacccaaac ggggactgcg agttcgtagt ggacatcgtc ttcgacacgg gtgaacagcg
     6601 gatgcggagc gatctgcacg gtcaggtaga ggtccccgcc ctgcccctgc ccggccgccc
     6661 ggatgcgctg gccgtgccgc actccggggg gaatcgtcac ctcgaggccc cgcgtcttcc
//
LOCUS       DASQGH010000528         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55857, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000528 DASQGH010000000
VERSION     DASQGH010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55857"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VEW91_10385"
     CDS             complement(<1..183)
                     /locus_tag="VEW91_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92028.1"
                     /translation="MRSWTRPLGILLLAGAVLVCPRNLPPTQAGPQHVLPGNILIADE
                     GNNRILEITPDKKIVWE"
     gene            complement(221..583)
                     /locus_tag="VEW91_10390"
     CDS             complement(221..583)
                     /locus_tag="VEW91_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYM92029.1"
                     /translation="MRPLALAVTLGLVALVGLAVPAQTAPGTGTFAVVNVEVSGVKIW
                     LPSAIVVRPGQQVTLKLDNKLDAPHGFSIDDYAIQVVVPAGGTQDVTFTAKRGLTSRF
                     YCQIHPPHVGGQIIVISN"
     gene            complement(761..1960)
                     /locus_tag="VEW91_10395"
     CDS             complement(761..1960)
                     /locus_tag="VEW91_10395"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HYM92030.1"
                     /translation="MASVRPLTDLTVADLWREVKDPATLWGDVSRQTLQTVKRLLENR
                     MHDELAEHLKAHRYARTAYRRGYRNGRYIRRLTTTWGTIPNLEIPRPRTGRFTPTVLD
                     RYQRRMPQVDELVRRVFLGGLSTRQVGPVLAQLLQDTVSPTTVSQITQTLDRQVARFH
                     RRPLPDHYHYLFLDAVSLRVKTPDGTRRRHALVAYGITTQGQRELIDYRLVRQEGSSA
                     WEAFLVNLACRGLTGAALRLITTDGHRGLHAALALAYPHVPRQTCWVHVLRNVAQRLR
                     VRDRDACLALARRIYQARSQHAAARALQRWRQAWEPTAPKAVACVLRDWEALLAFYTV
                     PEPDWRRVRTTNAIERAFREIRRRTRPMTCFTNTTSCDRIMYAVIHALNLRRAGRPLS
                     WETTQNS"
BASE COUNT          348 a          637 c          873 g          385 t
ORIGIN      
        1 ctcccagacg attttcttgt ccggggtgat ctcgaggatc cggttgttcc cctcatccgc
       61 gatgaggatg ttgccgggga ggacgtgctg cgggccggcc tgggtggggg ggaggtttcg
      121 gggacacacg agcaccgccc ccgccaggag aaggatcccg agcggccggg tccatgagcg
      181 caaggtggca cacccctggt tgataccgcg cctccgtcgt ctaattcgag ataacgatga
      241 tctgccctcc gacgtggggc ggatggattt ggcagtagaa gcggctggtg agcccacgct
      301 tggcggtaaa cgtgacatcc tgcgtgcccc ctgccggcac cacaacctgg atcgcgtagt
      361 catcgatgga gaacccgtgc ggggcgtcca gtttgttgtc gagcttcagg gtcacctgct
      421 gcccgggacg cacgacgatc gccgagggca gccagatctt tacgccgctc acttccacgt
      481 tgacgacggc aaaggtcccg gtgccgggcg ccgtctgcgc cggtacggca aggccgacga
      541 gcgcgaccag gccgagcgtc accgccagag cgagcggacg caccgcaccg cgcgtcccat
      601 gtggaacccg catcatttga tctccccctt ctcgggctgg ggcggttcga gcaggctcga
      661 tgtttccacc ccatccccgg ctccccctgc cgtgccggcg gcgccggccg gccgcccacc
      721 ggcgagccca agcgtggatc cggccacagg aagggtagtg tcaagagttt tgtgtagttt
      781 cccatgagag aggacggccg gcccgacgta gattgagggc gtggatcacg gcgtacatga
      841 tccggtcgca gctcgtagtg ttggtaaagc aggtcattgg gcgggtgcgg cggcggattt
      901 cgcgaaaggc gcgttcgatg gcgttggtgg tccggacgcg gcgccagtct ggctcgggga
      961 cggtgtagaa ggccagcagg gcttcccagt cgcggagcac acaggctacg gccttggggg
     1021 cggtgggttc ccaggcctgc cgccagcgct ggagggcgcg cgcggcggcg tgctggctac
     1081 gggcctggta gatgcggcgg gcgagggcta agcaggcgtc ccggtcgcgg acccgcaggc
     1141 gttgggcgac gttgcggagg acgtggaccc agcacgtttg gcgggggacg tggggatagg
     1201 ccagggccag ggccgcgtgg aggccgcgat ggccatcggt ggtgatgagg cggagggccg
     1261 cgccggtgag gccgcggcag gcgaggttga cgaggaaggc ctcccaggcg gaagagccct
     1321 cctggcggac caggcggtag tcgatcaatt cccgctggcc ctgcgtggtg atcccgtagg
     1381 cgacgagggc gtggcggcgg cgtgtgccgt ccggggtttt gacgcggagg ctgaccgcat
     1441 cgaggaagag gtagtggtag tggtcgggga ggggccggcg atggaagcgg gccacctggc
     1501 gatcgagggt ctgggtgatc tgggagacgg tggtgggact gacggtatcc tggaggagct
     1561 gggccaggac cgggccgacc tggcgggtgg agaggccgcc gaggaagacg cggcggacga
     1621 gctcatcgac ctggggcatg cggcgttgat agcggtccaa gacggtgggc gtaaagcggc
     1681 cggtgcgggg gcgggggatc tcgagattgg ggatggtgcc ccaggtggtg gtgaggcggc
     1741 ggatgtagcg gccattgcgg tagccccggc gataggccgt gcgggcgtag cggtgggcct
     1801 tgaggtgctc ggccagttcg tcgtgcatgc ggttctctaa gaggcgcttg acggtctgga
     1861 gcgtctggcg ggagacatcg ccccagaggg tggcggggtc cttgacctcg cgccacagat
     1921 cggctacggt gagatcggtc agtggtcgca cactggccac gggtgtatcc ctcctttatc
     1981 tgggtggcgc tgggtggggg cggggtacgc cctccttatt tgcgctcctg gcttttacac
     2041 agaatatcgt acactaccgc aggacgcgga tttgacgcga tctgcggaaa ctgatacact
     2101 ttaaaagtcg tcatacatgc gttctgcgag ggatgccaga ggcggatccc tccttgtgtg
     2161 tcgcaggcct tcttcatggg gagtaggttc cggcgagaca cgcgcaggtg ccggcaccga
     2221 ttcctgcggc agagccgcac ccg
//
LOCUS       DASQGH010000529         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13820, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000529 DASQGH010000000
VERSION     DASQGH010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13820"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VEW91_10400"
     CDS             complement(<1..812)
                     /locus_tag="VEW91_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYM92031.1"
                     /translation="MRPYWRGHISFGLVTFPVKLYTATEEKDVRFRLLHRECLTPIKN
                     QRYCPHHDAVIEWKDVVRGYEVSKGKFVTVTDEEFEGIPIDTSHSVNIAGFIELPEID
                     PLYYEHSYYVAPDEGGAKAFVLLRDALKEVNRVAVGQVVIREKEYPVALRPYEGAMVM
                     ATLYYADEVRALSALEEFPVEAKVHPNERKMAIQLVENLAMEFQIAEFRDEYREKLLA
                     MIKAKAEGEAVVTPKAAAPGKVIDLMEALRRSVEMAQGSRKAEAGRGGAQRRA"
     gene            complement(947..>1631)
                     /locus_tag="VEW91_10405"
     CDS             complement(947..>1631)
                     /locus_tag="VEW91_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM92032.1"
                     /translation="PDRVGETLMGLGNVLSTSGELEGALSLYERARTLFEQHEDLQAA
                     AHVRHSLGIVLMQTGRPRESLEHFQESLASMQRLEDGAGECRALTELARCYFVLGERA
                     PAAEFAERALARSRAAGQPDEGARARIVLGMLAAERGDTEAAAGALQEAAAQCRRSGM
                     MAELVAICHELARLAGREGHYKEAAAYHEQAHDALRAVKPTDVVAAVHLADLVGLKAE
                     AAKGSKRPR"
BASE COUNT          231 a          617 c          494 g          289 t
ORIGIN      
        1 gcccttcgtt gcgcgccgcc ccggccggcc tcggcctttc ggctcccctg ggccatctcc
       61 acgctgcgcc ggagggcctc catgagatcg atgaccttcc cgggcgccgc ggctttcggg
      121 gtcaccaccg cctccccctc ggccttggcc ttgatcatgg ccagcagctt ctcccgatac
      181 tcgtcgcgga actccgcgat ctggaactcc atcgccaggt tctcgacgag ctggatcgcc
      241 atcttgcgct cattggggtg gaccttggct tccacgggga actcctcgag cgccgagagc
      301 gcgcggacct catcggcgta atacagggtc gccatcacca tggcgccctc gtacggccgg
      361 agggcgaccg ggtattcttt ctcgcggatc accacctggc ccaccgccac gcggttgact
      421 tccttgagcg cgtcccggag gagcacaaac gccttggccc caccttcgtc cggggccacg
      481 taataggagt gctcgtaata cagcgggtcg atttcgggca gttcgatgaa ccccgcaatg
      541 ttcacgctgt ggctcgtgtc gatcgggatc ccctcgaact cctcgtccgt gacggtcacg
      601 aacttcccct tgctgacctc gtacccccga acgacgtcct tccattcgat gaccgcgtca
      661 tggtgggggc agtaccgctg gttcttgatc ggggtgaggc actcgcgatg gaggagccgg
      721 aaccgcacgt ccttctcctc ggtcgccgtg tagagtttga ccggaaacgt gacgagcccg
      781 aagctgatgt gcccgcgcca gtacggtctc accggtgctc cctcccgtcg tgccgggtga
      841 aaacgggtta ccccctcctg cgaagcggta ccgaggattg tagtacccgt tggaagggta
      901 cgcaagttcg aaggcaaacg tgtgttcgca aattggcgcg gggcgattac ctggggcgct
      961 tcgaaccctt cgcggcctcg gccttcaggc cgaccagatc ggccaggtgg accgcggcca
     1021 ccacgtcggt gggcttcacg gcgcgcagcg cgtcatgcgc ctgctcatgg tacgcggccg
     1081 cctccttata gtgcccctcc cggccggcta ggcgcgcgag ctcatggcag atcgccacga
     1141 gttccgccat catccccgac cgccggcact gcgccgcagc ctcctgcagc gccccggccg
     1201 cggcctcggt gtccccgcgc tcggcggcga gcatccccag gacgatccgc gcccgcgccc
     1261 cctcgtccgg ctgtcctgcc gcacggctcc gcgcgagcgc gcgctcggca aactccgccg
     1321 ccggcgcgcg ctcgcccaag acgaagtagc accgcgccag ctccgtcagg gcccggcact
     1381 cacccgcccc gtcctccagt cgctgcatgc tcgcgaggct ctcctggaag tgctccagcg
     1441 actcccgcgg ccgtcccgtc tgcatcagga cgatccccag gctgtgccgc acgtgcgcgg
     1501 cggcctgcag atcttcgtgc tgctcgaaca acgtgcgcgc acgctcgtac aggctgagcg
     1561 ccccctcgag ctccccgctc gtcgagagga cgttccccag tcccatcagg gtctccccca
     1621 cccgatccgg c
//
LOCUS       DASQGH010000530         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30736, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000530 DASQGH010000000
VERSION     DASQGH010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30736"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VEW91_10410"
     CDS             <1..209
                     /locus_tag="VEW91_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92033.1"
                     /translation="ISIPCGFTQGGLPVGLQIVGRRRQETTVLTAAAAFEQAAPWAGH
                     LPPVVKSSTQSREPRAERGGHASA"
     gene            407..1516
                     /locus_tag="VEW91_10415"
     CDS             407..1516
                     /locus_tag="VEW91_10415"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HYM92034.1"
                     /translation="MKKDITHVALDDSKNSIVAGILRPQATEPELCQFPNEPKPLRRF
                     FARLQREGPLATCYEAGPSGYDLYRQLTALGVPCQVMAPALTPRKPGERIKTNRRDAA
                     KLVRLFRAGELTPIRIPDEAQEAVRDLLRCREDVQEDLLRWRHRLLKFLARHGRVYRE
                     GRHWTQRHWTWLRGQRFALPALERTAQEYRFTVEQLLARRAELARALEAIAQQEPYRT
                     PVGWLRCFRGIDTLGALTLLIEIGDFPRFQRPRELMAYLGLVPSEASTGDRERRGSIT
                     KAGNTHARRILVEAAWAYRHPPRLGAALRRRSQGQPKAVLAQAWRAQQRLHRRYRHLV
                     GHGKRPPVAVVAIARELVGFLWAALTRAEPAAQAA"
BASE COUNT          315 a          709 c          699 g          268 t
ORIGIN      
        1 caatctcgat cccgtgcggc ttcacgcagg gcggcctgcc cgtcggcctc cagatcgtag
       61 gtcggcggcg gcaggagacg acggtcctca ccgccgccgc ggccttcgag caggcggcgc
      121 cgtgggcggg gcacctcccg ccggtggtga aaagcagcac ccagagccgg gagccgagag
      181 cggagagggg agggcatgcg tccgcctagc aggatgctga aaaacccgtt ttcgcccagg
      241 ctgctcaaaa aggtccagat gcaaggcggc gcgccgggta cccatccgga ggatgggtgc
      301 aggtgcgagg cgtacttaga ccatatggaa agaccggccc acctatcgca gaatagcgtg
      361 ggttgttccg ggacggaaca gagccacatg cgaaaggagg ccggtcatga aaaaggatat
      421 cacacacgtg gccctcgatg acagcaagaa ctcgatcgtg gcggggatcc tgcggcccca
      481 ggcgacggag cccgagctgt gccagttccc gaatgagccc aagcccctcc ggcgcttctt
      541 cgctcggttg cagcgggagg ggccgctggc gacttgctat gaagcggggc cctcgggata
      601 cgatctctac cggcagctca ccgctctcgg cgtcccctgc caagtcatgg ccccggccct
      661 caccccccgc aagcccgggg agcggatcaa gacgaaccgc cgggatgccg ccaagctcgt
      721 ccgcctcttc cgggccgggg agctcacccc aatccgcatc cccgacgaag cccaggaggc
      781 ggtgcgggat ctcctccgct gccgggagga tgtccaggaa gatctcctcc gctggcggca
      841 ccggctgctg aagtttctcg cccgccacgg ccgagtgtac cgggaggggc gccattggac
      901 tcagcggcac tggacctggc tccggggcca gcgatttgcc ctgcccgcgc tcgaacggac
      961 cgctcaggag taccgcttca ccgtcgagca gctccttgcc cggcgggcgg aactcgcccg
     1021 ggcgctcgag gccatcgccc agcaggagcc ctaccggacc ccggtgggct ggctccggtg
     1081 cttccggggc atcgataccc tcggggccct gacgctcctg atcgagatcg gggacttccc
     1141 gcgcttccag cggccccggg aactcatggc ctacctcggc ctggtcccga gtgaggcctc
     1201 caccggcgac cgggagcggc gcgggagcat caccaaggcc ggcaataccc acgcccggcg
     1261 gatcctggtc gaggcggcct gggcctaccg gcacccgccc cgcttggggg ctgcgcttcg
     1321 gcgccggagc caggggcagc cgaaagcggt cctcgcccag gcgtggcggg cccagcagcg
     1381 cctccaccgg cggtaccggc atctggtcgg gcacggcaag cggcccccgg tggcggtggt
     1441 ggcgatcgcc cgggagttgg tcggcttcct ctgggccgcc ctgacgcggg ccgagcctgc
     1501 cgcccaggcc gcgtaggact cggaccacgc ggagcgatgg gatactatcc tgaggcccca
     1561 ggggacacgg gcgcgatgca ggcacgattg gagagccctc gctcggacta tgcgacactc
     1621 gggctgcggc ccgggggact cgcgcgttta gacggaggca gctccccgac ggagcacggt
     1681 catgcggcct ccggtcgccg accggagcga cccgcgcata tcagagtgat tcgtcgtcga
     1741 agccgcctcg tcggcgcccg gccccggggc ctcaggacac cctccgccgc gctgcagctg
     1801 caccgcggga ccctgcccgc cgagcgcgct gcgccacacc ttcgggcgcg ccgcgcgccg
     1861 cggcgggcgt acggatgttg ccggaggagc agaaccaaac gataatcctt gacggccggt
     1921 cttccatatc agtccgtacg tcgcagcgcc gcgcgagcgc gggggtaccc accgaaggtg
     1981 ggtctccgca g
//
LOCUS       DASQGH010000531         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53212, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000531 DASQGH010000000
VERSION     DASQGH010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53212"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VEW91_10420"
     CDS             complement(<1..639)
                     /locus_tag="VEW91_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM92035.1"
                     /translation="MNHRYAVRMLALTAALAAVALFPGTPLRAAGPTQVSYWFPVDLG
                     GGLANVMQTLVAQFNQTHPDIRVTASYTGNYDATLQKIQAAQLAGGFPDVAVIVNEHT
                     QLLAPTGILAELDGFVAKAGGKAYLDRIFPALLLNSYYKGKLYSLPYQRSVPVLYYNK
                     DAFKEAGIGQPPATWADLIADAQKLTIRQGSQVTRWGAEFPLEAYNWIYYALI"
     gene            complement(829..1224)
                     /locus_tag="VEW91_10425"
     CDS             complement(829..1224)
                     /locus_tag="VEW91_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM92036.1"
                     /translation="MRIVRVQPEHAHAPQNPEYFSGTVRQQRLTTEPGGIDLVAVFFE
                     PGARTRPHIHATDQALHVVEGEGIVATEQERRLIRAGDIVIIRAGQWHWHGATPTSAM
                     CHISARPAGPTDWSAPLRDWEAYMQGVRG"
     gene            complement(1280..>1915)
                     /locus_tag="VEW91_10430"
     CDS             complement(1280..>1915)
                     /locus_tag="VEW91_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HYM92037.1"
                     /translation="CVNALEEYQQGRIYIGPELMGYQETNGRLVRLPESQVSRQRAEV
                     GMVFQSYNLFPHMTVLDNVALGLVHVQKKARAEARQTALSWLERVGLADKVKAYPFEL
                     SGGQQQRVAIARAVAMQPKVMLFDEVTSALDPELIGEVLIVMQDLARSGQTMLVVSHE
                     MIFVREVAHRVVFMGDGIIVEEGPPSETLVRPRTERLQAFLSRFRGVFGPV"
BASE COUNT          306 a          639 c          625 g          345 t
ORIGIN      
        1 gatgagcgcg taatagatcc agttgtacgc ttcgaggggg aactcggccc cccaccgggt
       61 gacctggctg ccctgacgga tggtgagctt ctgggcgtcg gcgatgaggt cggcccaggt
      121 cgccggtggc tggccgatcc ccgcctcctt gaaggcgtcc ttgttgtagt agaggacggg
      181 tacgctgcgc tggtagggga gcgagtacag cttccccttg tagtagctgt tgagcaggag
      241 cgccgggaag atccgatcca ggtacgcctt cccccccgct tttgcgacaa atccatcgag
      301 ctccgcaagg atgccggtcg gcgcgagcag ttgggtgtgc tcgttgacga tcaccgcgac
      361 gtccggaaag ccgccggcga gttgcgcggc ctggatcttc tgaagcgtcg cgtcgtagtt
      421 gccggtatag ctcgccgtga cgcggatgtc cgggtgggtc tggttgaact gggccaccag
      481 ggtctgcatc acgtttgcga gtccccctcc gaggtccacg gggaaccagt agctgacctg
      541 ggtcggtccc gcggcgcgca gcggggtccc gggaaagagc gcaacggcgg ccagggccgc
      601 cgtgagggca agcattcgaa ccgcataccg atggttcatt gaagttcctc ctccccagct
      661 aatgggatcg ggcgatgttc gaccggacgt cctacggcga tccggccagc atggcgttgc
      721 ccttggcggt ccggccgccc aggccagcca gggtgggttc ctgcttcgac gcctcggctg
      781 cgcattcttg ccatactcga gcccgcgatc ctccgcttgg ggagtctatc agccgcggac
      841 gccttgcatg tacgcctccc agtcgcgtaa cggggcggac cagtctgttg ggccagcggg
      901 ccgcgcagag atgtggcaca tcgcagaggt cggcgtggcc ccatgccagt gccactggcc
      961 cgcccggatg atgacgatgt ctcccgcccg aatgagtcgt cgctcctgct cggtcgcgac
     1021 gatcccttca ccctcgacga cgtggagggc ctggtcggtc gcatggatat ggggccgcgt
     1081 ccgcgcgcca ggctcaaaga agaccgcgac cagatctatt ccccccggct ctgtcgtcag
     1141 gcgctgttgg cggaccgtgc cgctgaaata ctctggattt tgcggagcgt gcgcgtgttc
     1201 cggctgcact cggactattc gcattcgtgc ccccctgcct ttcaggcgag attgggcaaa
     1261 cgcaccgccg agtgcccacc tagacggggc cgaaaacccc gcggaagcgg ctgaggaacg
     1321 cctggagacg ctcggtgcgc ggacgaacaa gagtctcgct cggcggcccc tcctcgacga
     1381 tgatgccgtc gcccatgaac acgacccgat gggccacctc gcgaacgaag atcatctcgt
     1441 gcgagacgac cagcatcgtc tggccgctgc gggccaggtc ctgcatcacg atcagcacct
     1501 cgccgatcaa ttccgggtcg agggccgaag tcacttcatc gaagagcatc accttgggct
     1561 gcatcgcgac cgcccgggcg atcgcgacgc gctgctgctg ccccccggag agctcgaatg
     1621 gatacgcctt caccttgtcc gcgagcccca cgcgctcgag ccaggacagg gcggtctgtc
     1681 gcgcctccgc ccgcgccttc ttctgcacgt gcacgagccc cagggccacg ttgtcgagca
     1741 cggtcatgtg gggaaagagg ttgtagctct ggaacaccat ccccacctcg gcgcgctgcc
     1801 gcgagacctg cgactcgggg agccggacca gccgtccgtt cgtctcctga taccccatca
     1861 gctctggccc gatgtagatc cggccctgct ggtactcctc cagggcattc acgca
//
LOCUS       DASQGH010000532         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47727, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000532 DASQGH010000000
VERSION     DASQGH010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47727"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..400)
                     /locus_tag="VEW91_10435"
     CDS             complement(14..400)
                     /locus_tag="VEW91_10435"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYM92038.1"
                     /translation="MARVDLALLLLRVILAAAFGAHGYLKWAGGLGPVAGLMMTVHLP
                     FPDFLARVSATLELGGAVLLLVGLWTRLIGALLAVEMAVAIVKVVWPQGFIGGFELEL
                     TLLTVALALAIAGGGTLSVGRGRLSR"
     gene            complement(425..913)
                     /locus_tag="VEW91_10440"
     CDS             complement(425..913)
                     /locus_tag="VEW91_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM92039.1"
                     /translation="MRTLAYALAGNLLAIALATGGAFAHDSEPTTTSPTTQVNAMAKT
                     YFDQAEGYSKKKDWNLAIAAYQQAVRIDPKFVTAWSNMGYCYRKVNQFDKAMDAYKRA
                     LTLKPDYTYPHEYLGRTYLAMGNKDAAMREYEILKRLDAKMAAGLLKAIEANNADLGD
                     ED"
     gene            complement(993..3137)
                     /locus_tag="VEW91_10445"
     CDS             complement(993..3137)
                     /locus_tag="VEW91_10445"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HYM92040.1"
                     /translation="MHPLVLSLAIHNHQPVGNFDHVIAKATDSAYAPFVGALERHPRI
                     RLALHYSGPLLDWLKPHRPDLLQRIRMLVARGQIEILTGGYYEPILAVIPDADKRGQI
                     EMLTRVVAAEFGCHAEGLWLAERVWEPHLARPIAEAGARYTIVDDTHFYAVGLEEGQL
                     LGYYLTDEAGVPLAIFPSLKRLRYLIPWAPVEDVIGYLRTLAEREVRPDGRRAVLDLA
                     LMGDDGEKFGLWPGTHAHCWEHGWIDRFFEALERTPWIEVVPPGEYRRTREPAGRIYL
                     PTASYDEMGEWALPPARSTDLAHLRHGLETSQWAGGLPFVRGGFWRHFLVKYDEINTL
                     YRLSLRAGAKVHAMPPGPEKTRALEALWAGECNCTYWHGVFGGVYLPHIRGAAFSHLI
                     AAEALAEAATHPRRFALAETADLDGDGRPDVRLATELLALTVDPSRGGSVVEWDSKPA
                     RRHLGNVLTRRREGYHAGLLEALASGAVQIAGQADLETIHTTAVRVKEPGLERFLIYD
                     RQRRASLRFHLLPRDTTLEQMWHDRQEDLGGFAADAYAWELEEAPGRIAVRLRRLAEL
                     GGAQVSAERTIEVASGAHGLVPRTRLRSPGTAMAPTLLAEEWGLGLFGAPGEVWAEAG
                     GRRIPLHEPGALPASERVAVGETHSGLVLTFELSHPATVWAFPLVTISNSESGYEQNF
                     QGAVLVLCRPVELASGRGVDQATRCAITGVPA"
     gene            complement(3146..>3603)
                     /locus_tag="VEW91_10450"
     CDS             complement(3146..>3603)
                     /locus_tag="VEW91_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92041.1"
                     /translation="RVDPAVAGPHDRARLGHGLTATMFMIAMQNAVTRAQRGLATSVS
                     IFSRNIGSAIGVSLQGAVLVGVLMARLRIGSVRITNPQAALDSTFQAGLTPAGHEAFR
                     LALAQALHGTFLLGLALVLGGLVLVLVYMPSGGVLELSPVRAAGGGEAD"
BASE COUNT          538 a         1277 c         1256 g          532 t
ORIGIN      
        1 gcgccggccg ccgttaccga gaaaggcggc cgcgcccgac cgatagcgtt cccccgccgg
       61 cgatggcgag ggcgagcgca acggtcagca gcgtcagttc gagctcgaac cctccgatga
      121 acccttgggg ccagacgacc ttgacgatgg cgaccgccat ctccaccgcc agcaacgcgc
      181 cgatcaggcg ggtccagagc ccgacaagca gcaggacggc gcccccgagt tccagggtgg
      241 cggacacgcg cgcgaggaag tcggggaagg ggaggtggac ggtcatcatc agccccgcca
      301 cgggccccag cccgccggcc cacttgagat agccgtgcgc tccaaaggcc gcggccagga
      361 tcacccgcag aagcaagagg gcgaggtcga ccctggccac gcgcgctcag ccggatgcgc
      421 ccgtctagtc ctcatccccc aggtcggcgt tgttcgcctc gatcgctttg agcagtcccg
      481 ccgccatctt ggcatccagc cgcttgagga tctcgtattc ccgcatcgcc gcgtccttgt
      541 tgcccatggc gaggtacgtc cgccccaggt actcgtgggg ataggtgtag tccggcttca
      601 gggtgagggc gcgcttgtac gcgtccatgg ccttgtcgaa ctggttcact ttgcggtagc
      661 agtagcccat gttgctccac gccgtgacaa acttggggtc gatgcgcacc gcctgctggt
      721 acgccgcgat cgctaaattc cagtccttct tcttggagta cccctccgcc tggtcgaagt
      781 aggtcttggc catcgcattg acctgggtgg tcgggctggt ggtggtcggc tcggagtcgt
      841 gcgcaaaggc tccccccgtc gccagggcga tggccagcag gttacccgcg agcgcgtacg
      901 caagcgttct catcaccgca cctcggtgga gacgacgttc ggtgtcccca ctgtgcacga
      961 ccactgtcac gggcgtatca caggtcgccg cctcacgcgg gcacgccggt gatcgcgcac
     1021 cgcgtcgcct gatccacgcc ccggcccgag gccagctcga cgggccggca gagcaccagc
     1081 accgccccct ggaagttctg ttcgtatcca ctttcggagt tcgagatggt gacgagggga
     1141 aacgcccaga ccgtcgcggg atgggagagt tcgaacgtca ggacgagccc cgagtgggtc
     1201 tccccgaccg ccacgcgctc ggatgcgggc agcgctcccg gttcgtgcag gggaatccgg
     1261 cggcctcccg cttcggccca cacctccccc ggggctccga acaacccgag cccccactct
     1321 tcggccagga gcgtgggcgc catcgcggtg ccaggcgagc gcaggcgggt gcggggcacc
     1381 agcccgtgcg cgcccgatgc cacctcgatg gtgcgctccg ccgagacctg tgcgccccca
     1441 agctctgcga ggcggcgcag ccgcaccgcg atgcgcccgg gcgcctcctc gagctcccac
     1501 gcgtaggcat cggcggcgaa cccgccgaga tcttcctgcc ggtcgtgcca catctgctcg
     1561 agcgtggtgt cccggggcag caggtggaag cgcaggctcg cccggcgctg ccgatcgtag
     1621 atcaggaacc gctcgagccc gggctccttg acccgcaccg cggtggtgtg gatcgtctcc
     1681 agatcggcct gcccggcgat ctggaccgcg cccgacgcga gcgcctcgag gaggccggcg
     1741 tggtagcctt cgcgccgccg cgtcagcacg tttcccaggt ggcggcgcgc gggcttggaa
     1801 tcccactcca ccacgctccc cccccggctg ggatcgacgg tgagggccag gagttccgtg
     1861 gccaagcgca cgtccggccg gccatccccg tccagatcgg ccgtttcggc gagcgcgaac
     1921 cgccggggat gggtggcggc ctcggcgagg gcttcggcgg cgatcagatg gctgaacgcg
     1981 gccccccgga tgtgcggcag gtagacgccg ccaaacaccc cgtgccagta cgtgcagttg
     2041 cattcgccgg cccacaacgc ttcgagcgcg cgcgtcttct ccggcccggg gggcatggcg
     2101 tggaccttgg ccccggcgcg caggctcagc cggtacaggg tgttgatctc gtcgtacttg
     2161 acgaggaagt ggcgccagaa gcccccgcgg acgaacggga gcccgcccgc ccactggctg
     2221 gtctcgagcc cgtgcctgag gtgcgcgagg tcggtcgacc gcgccggggg gagcgcccac
     2281 tcgcccatct cgtcgtacga cgccgtgggg aggtagatgc gtccggcggg ttcgcgtgtg
     2341 cgccggtatt cgccgggcgg gacgacctcg atccacggtg tccgctcgag cgcctcgaag
     2401 aagcggtcga tccacccatg ctcccagcag tgggcgtgcg tgcccggcca gaggccgaac
     2461 ttctccccgt cgtcccccat cagcgccagg tcgagcacgg cccggcggcc gtccggccgg
     2521 acctcgcgct ccgcgagcgt ccgcaggtac ccgatcacgt cctccaccgg cgcccagggg
     2581 atgaggtagc gcaaccgttt caggctgggg aagatcgcca ggggcacgcc cgcctcgtcg
     2641 gtgaggtagt agccgaggag ctgcccctcc tcgagcccga cggcgtagaa gtgggtgtcg
     2701 tccacgatgg tgtaccgcgc gcccgcctcg gcgatcggcc gcgcgaggtg gggctcccat
     2761 acccgctcgg cgagccagag gccctccgcg tggcacccaa actccgcggc caccacccgg
     2821 gtgagcatct cgatctgccc gcgcttgtcc gcatccggga tgacggccag gatcggctcg
     2881 tagtagccgc cggtcaggat ctcgatctgg ccgcgcgcga ccagcatccg gatccgctgg
     2941 aggaggtcgg gccgatgggg cttgagccag tcgagcagcg gtccggagta gtgcagcgcc
     3001 agccggatgc gcgggtggcg ttccagcgcg cccacgaacg gagcgtacgc gctgtccgtc
     3061 gccttggcga tgacgtggtc gaagttgccg acgggttgat ggttgtggat tgccagtgag
     3121 aggaccagtg ggtgcatcgc ggcagtcagt cggcttctcc acccccggcc gcgcggaccg
     3181 gcgacagttc cagcacgccg cccgacggca tgtagaccag caccaggacg aggccgccga
     3241 gcacgagcgc gaggccgagc aggaaggtgc cgtggagcgc ctgggcgagc gcgagccgga
     3301 acgcctcgtg cccggccggc gtgaggccgg cctggaaggt ggaatccagg gccgcctgcg
     3361 ggttggtgat ccggacgctc ccgatcctca accgggccat cagcaccccg accaggaccg
     3421 cgccttggag gctgaccccg atcgcgctgc cgatgttgcg cgagaagata ctgaccgacg
     3481 tcgcgagccc tcgctgcgcg cgcgtcaccg cgttctgcat ggcgatcatg aacatcgtgg
     3541 ccgtgagccc atgcccaagc cgagcacgat catgggggcc agcaactgcg gggtcgacgc
     3601 gtg
//
LOCUS       DASQGH010000533         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33524, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000533 DASQGH010000000
VERSION     DASQGH010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33524"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VEW91_10455"
     CDS             complement(<1..313)
                     /locus_tag="VEW91_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYM92042.1"
                     /translation="MGQRGRKIVNLKVEDLLADLKKVYLDELLAFYSYWITAEVAEGF
                     HGEELTEHFQEEAKDELRHAQKWAKRITELGGDPVIHPSEWEAQANGPWTAPRRDRSD
                     AD"
     gene            complement(382..>2374)
                     /gene="selB"
                     /locus_tag="VEW91_10460"
     CDS             complement(382..>2374)
                     /gene="selB"
                     /locus_tag="VEW91_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HYM92043.1"
                     /translation="GTPRHLPPSAAKRPAPTVPPSSAPPRHPPRYAVVGTAGHIDHGK
                     SALVKALTGIDPDRLEEEKRRGMTIDLGFAHFDLPSGLRVGLVDVPGHERLIRNMLAG
                     ATGIDLVLLVVAADEGVMPQSREHLDILRFLRVERGIVVLNKVDLVADPEWRQLVIED
                     IRALVAGTFLENAPCIPVSARTGAGIPQLVAAIDESLQAISPRPVDAPVRIPIDRSFT
                     MAGFGTVVTGTLWTGRVRTGDALELLPLGREVRVRQLQSHGVVVDEACAGQRVALNIV
                     GAAKAEIARGHVLAFPRSLQPTALLDVRVRLLAGAPPLSHHERVRMYVGADEVIGRLR
                     LLDRGRLDPGAAAVAQLRLERPTVVARGDPFVLRRYSPMITVGGGEVITARAALRRRG
                     ALAAQAVAAEEGSGFDDRLVAALLAAGRSGSTVEALAMEVGASRDRVASRTLAWTAAQ
                     EIIDLRSRLFSARVAQQIQSDVLAALAAHHAATPWRRGVPRDELKTRVFGSGDDRLYG
                     YALDALALGGRIAIEDGFVRLAEFSPSRSAAETTARRAIEEAFRSGRYAPPAREDALA
                     AIADRAVADRMLQALLDDGLLVTVGDEILFHREVLRELATRVAAHIGEHGEITVAALR
                     DQLGTSRKYTLAVLEYFDAIHLTRRVGDRRVLARPAPRP"
BASE COUNT          345 a          875 c          808 g          346 t
ORIGIN      
        1 cgtccgcgtc gctcctatcg cgtcgcggag ccgtccacgg accgttcgcc tgcgcctccc
       61 actctgaagg gtgaatcacc ggatcgcccc ccagctcggt gatccgcttg gcccactttt
      121 gcgcgtgcct caactcatcc ttcgcttctt cctgaaagtg ctccgtgagc tcctcaccgt
      181 gaaacccctc cgcaacctcc gcggtgatcc agtacgagta gaacgcgagg agctcgtcca
      241 ggtacacctt cttgaggtcc gcgagcagat cctcgacctt cagattgacg atcttcctac
      301 ccctctgccc cattcctatc cctccctccc atgattgccg taccgggcac gtctacacgc
      361 cgcgcgctgc cgttcccacg atcagggccg cggtgcgggc cgggccagca cacgcctgtc
      421 cccgacccgt cgggtcaggt ggatggcgtc aaagtattcg agcaccgcca gggtgtactt
      481 tcggctggtg cccagctggt cccgcagcgc tgcgaccgtg atctccccgt gctcgccgat
      541 gtgcgccgcc acccgcgtcg cgagctcccg aaggacctcg cgatggaaga ggatctcgtc
      601 gccgacggtg accagcaggc cgtcgtcgag cagggcctgg agcatccggt ccgcgaccgc
      661 gcggtccgcg atcgcagcca gggcgtcctc ccgggcgggc ggggcgtacc ggccgctgcg
      721 gaacgcctcc tcgatggcgc ggcgtgctgt ggtctcggcc gcggatcgag acggggaaaa
      781 ctcggcaagg cgcacaaacc cgtcctcgat cgcgatccgg ccgcccagcg ccagcgcatc
      841 cagcgcgtac ccgtacaggc ggtcgtcgcc ggagccgaag acccgagtct tcagctcgtc
      901 gcgcgggacc cccctcctcc agggggtcgc cgcatggtgc gcggccagcg ccgcgaggac
      961 atcgctctgg atctgctggg ccacccgggc ggagaacagc cggcttcgca gatcgatgat
     1021 ctcctgcgcc gccgtccagg cgagcgtccg tgatgccacg cggtcgcggc tcgcgccgac
     1081 ctccatcgcc aatgcctcca cggtgctccc gctgcgtccc gccgcgagga gggcggcgac
     1141 gagacggtcg tcgaagcccg acccttcctc ggcggcgacg gcctgcgccg ccagggcccc
     1201 ccggcggcgg agcgcggcgc gggcggtgat gacctctcca cccccgaccg tgatcatcgg
     1261 ggagtagcgc cggaggacga acggatcgcc gcgggccacc acggtcggcc gctcgagccg
     1321 caactgggcc accgcggccg ccccggggtc gagccggccg cggtcgagca accggagccg
     1381 gccgatcacc tcgtcggccc ccacgtacat gcggacacgc tcgtggtggc tgagcggcgg
     1441 cgcgcccgcg agcagacgca cgcggacatc gagcagcgcg gtcggctgca gactgcgggg
     1501 gaaagcgagg acgtgcccgc gggcgatctc ggctttggcc gccccgacga tgttcaatgc
     1561 cacgcgctgg cccgcacatg cttcgtcgac cacgacgccg tggctttgca gttggcggac
     1621 gcgcacctcc cggcccaggg gcaggagttc gagcgcgtcg ccggtccgga cccgcccggt
     1681 ccagagcgtt ccggtcacca ccgtcccaaa ccccgccatg gtgaagctcc gatcgatggg
     1741 gatccgcacc ggggcgtcca ccggccgggg ggagatcgcc tggaggctct cgtcgatcgc
     1801 ggcgacgagc tgcgggatgc cggccccggt gcgggcggag accgggatgc acggcgcgtt
     1861 ttcaaggaac gtcccggcga cgagcgcccg gatatcctcg atgacgagct gccgccactc
     1921 cggatcggcg acgaggtcga ccttgttcaa gacgacaatc ccccgctcca cccgaaggaa
     1981 tctcaggatg tcgaggtgct cccggctctg cggcatgacc ccctcgtctg cggcgaccac
     2041 gaggaggacg aggtcgatcc cggtggcacc ggccagcatg ttccggatca gccgctcgtg
     2101 cccggggaca tccacgaggc cgacccggag gccgctcggc aggtcgaagt gggcgaaccc
     2161 caggtcgatc gtcatcccgc ggcgcttctc ctcctccagc cgatccgggt cgatgccggt
     2221 cagtgcctta acaagcgcgc tcttgccgtg gtcgatatgg ccggcggtcc ccacgaccgc
     2281 gtaccggggg ggatggcgcg gaggcgcgga agacggtggg accgtcgggg cggggcgctt
     2341 ggccgccgat ggtggaagat gacgcggggt accc
//
LOCUS       DASQGH010000534         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47729, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000534 DASQGH010000000
VERSION     DASQGH010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47729"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VEW91_10465"
     CDS             complement(<1..1265)
                     /locus_tag="VEW91_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HYM92044.1"
                     /translation="MGPRSVHEYAAALGPRYHIASRTEKKRLLTEFCRVTGRHRKSAI
                     RLLRRRAGSATPRPGRPRCYGPEVVALLRTVWEASDYLCGKRLAPFLPPLVDALERHR
                     VLQVPPPLRPVLLHLSPATIDRLLRPHRRGHPRGLATTGPGHPALAAQVPIRTFGDWQ
                     DVRPGSLQADLVGHCGESTEGFYLTSLVAVDVATGWTECQAVWGKGYARVGTGIHLIR
                     QRLPMPLRELHTDNGGEFLNHLLVPWCRREGIHVTRGRPWRKNDQAYAEQKNWAIVRR
                     LIGYDRYTTHAALDHLNALYRILRLYGNFFQPLRKLTAKVRHGARVTKRYDRAQTPYQ
                     RLLATAVLTPDQRHALDTLYRTLIPVALRTQIHDALRQVWQLADRPREAATPWEAHHA
                     NEAVKPSPTEARRAISRREDKISIPPAVRR"
     gene            1443..2075
                     /locus_tag="VEW91_10470"
     CDS             1443..2075
                     /locus_tag="VEW91_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYM92045.1"
                     /translation="MARTGRRPGASGTRQAILDAAREVLAREGYDRATLRGIARRAKV
                     DPALVLHYFHSKEALFVHAMRMPVNPAELMRGMLREGRADLGRRVMQTVLRIWSDRRN
                     AEALVGLFRSTMSNERAAAMLRQWLARAMLGSVRRSLRTPDAELRAALVASQILGLVV
                     TRHILRLKPLTSASTETLVDWIGPTIQRYLTGAAARADLVQARANLRVRF"
     gene            2078..>2589
                     /locus_tag="VEW91_10475"
     CDS             2078..>2589
                     /locus_tag="VEW91_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYM92046.1"
                     /translation="MRPRVTLIFRRLPPRARVSLLLLAAAAAAWAGWSWYEGVENLGR
                     IAADGSIEATIVHATTNVPGRVLRLVAGEGQGVKAGQVLAELEPQEADAQVAQARAAI
                     TQAEATLLRAQQAVTAQEQVTSAQVAQAQAAVTSATTGVPQSETQLAIQERTAREAVR
                     NAEAQLSAAQG"
BASE COUNT          398 a          800 c          993 g          398 t
ORIGIN      
        1 cgcctcacgg ccggggggat ggagatcttg tcctcccttc gagaaatcgc tctacgcgcc
       61 tctgtggggc tcggtttcac ggcttcgttg gcgtgatgag cctcccaggg cgttgctgcc
      121 tctctggggc ggtcggcgag ctgccacact tgacggaggg cgtcgtggat ctgggtccgg
      181 agcgccacgg ggatgagggt gcggtacagc gtgtcgaggg cgtggcgctg gtcgggggtg
      241 aggacggcgg ttgcgagcag gcgctgatag ggcgtctgcg cgcgatcgta gcgcttggtg
      301 acccgggcgc cgtgccggac cttcgcggtg agcttacgga ggggctggaa gaagttcccg
      361 tagaggcgca ggatgcggta aagggcgtta aggtggtcca gggcggcgtg ggtcgtgtac
      421 cgatcataac cgatgaggcg gcgcacgatc gcccagttct tttgctccgc ataggcctga
      481 tcgttcttgc gccacgggcg gccgcgggtg acgtggatgc cttcgcggcg gcaccagggg
      541 acgaggaggt gattgagaaa ctccccgcca ttatcggtgt ggagctcgcg cagcggcata
      601 ggcaggcgct ggcggatcag gtgaatgccg gtgccgacgc gtgcgtagcc tttgccccag
      661 accgcctggc attccgtcca gccggtggcg acatcgacgg cgaccaggga ggtcaagtag
      721 aaaccttcgg tactctcccc acaatggccg acgagatcgg cttggaggct gccgggccgg
      781 acgtcctgcc agtcgccgaa ggtgcggatg gggacctgcg cggcgagggc gggatgcccg
      841 gggcccgtgg tagcgaggcc ccgggggtgc ccacgccgat gcggccgcaa gagccggtcg
      901 atggtggcgg ggctgaggtg caggagcacc ggccggaggg gcggggggac ctggagcacc
      961 cgatgccgct cgagggcgtc cacgaggggc ggcaagaacg gcgcgagccg tttgccgcag
     1021 aggtagtcac tggcttccca gaccgtccgc aggagggcga ccacctcagg gccgtagcat
     1081 cgaggccggc cggggcgcgg cgtcgccgat ccggcacgcc ggcggaggag gcggatcgcc
     1141 gactttcgat gccgcccggt aacgcggcaa aactcggtga gcaagcgttt cttctcagtg
     1201 cgggaggcga tgtggtagcg tggaccgagg gctgcggcgt attcgtggac gcttcgggga
     1261 cccatgatgc ctcctccgcc cggtaacagg gttttgaggc atcagttcgt cgtccggtaa
     1321 cactatttat gaggcagatc gcggggttga caacttaatt caactaccgt agaattcaat
     1381 tggcgttgaa ttgtgagccg tggagcctgc gtgggacgta cggcagggag gcgtgaccgc
     1441 gaatggcacg gacgggccgc cggccggggg cgagtgggac gcgccaggcg atcctggatg
     1501 ccgcgcgaga agtgctcgcc cgagaagggt atgaccgggc cacactccgg gggatcgcgc
     1561 ggagagcgaa ggtcgatccc gcgcttgtcc tgcactactt tcacagcaag gaagcgctgt
     1621 tcgtccacgc gatgcggatg cccgtcaacc ccgccgagct catgcgcggg atgctccgag
     1681 agggtcgcgc ggacctcggc cggcgggtca tgcagaccgt cctgcgcatc tggagcgacc
     1741 ggcggaacgc cgaggcgctg gtcggcctct ttcgctcgac gatgtccaat gaacgagccg
     1801 cggcgatgct ccgccagtgg ctcgctcggg cgatgctcgg ctcggtccgg cgctcgctcc
     1861 gcacccctga cgccgagctc cgggcagcgc tggtggcctc tcaaatcctg gggttggtcg
     1921 tcacccgtca catcttgaga ctcaagccgc tgacgtcagc gagcaccgag acgcttgttg
     1981 attggattgg cccaaccatc cagcggtact tgaccggcgc cgccgcgcgc gccgacctcg
     2041 tgcaggcccg cgcgaatctc agggtgaggt tctgacgatg cgcccgcgcg tgacgctcat
     2101 cttccggcgg cttccgccgc gcgcgcgggt gagccttctc ctgctcgccg ccgccgcggc
     2161 cgcctgggcg gggtggtcct ggtacgaagg cgtcgagaac ctcggccgca tcgccgccga
     2221 cgggtccatc gaagcgacga tcgtgcacgc gacgacgaac gtgcccggcc gcgtcctccg
     2281 gctggtggcc ggcgagggac agggggtcaa ggccggccag gtgctggcgg agctggagcc
     2341 gcaggaggcc gacgcgcagg tggcgcaggc cagagccgcc atcacgcagg ccgaggcgac
     2401 tctcctccgg gcgcagcaag ccgtcacggc acaggagcag gtgacgagcg cgcaggtggc
     2461 ccaggcgcag gcggcggtga cctcggcgac cacgggcgtg ccgcagtcgg agacccagct
     2521 ggcgattcag gagcgcaccg cccgcgaagc ggtgcggaac gcggaggcgc agctgagcgc
     2581 ggcgcaggg
//
LOCUS       DASQGH010000535         5530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16446, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000535 DASQGH010000000
VERSION     DASQGH010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16446"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1411
                     /locus_tag="VEW91_10480"
     CDS             230..1411
                     /locus_tag="VEW91_10480"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYM92047.1"
                     /translation="MTEVVILDGARTPFGRFGGELRGVGATQLGIAAARGALAHSKVA
                     PEAVDHVVFGNVVQSSSDAVYLARHVGLGAGVRLDSPALTVNRLCGSGLEAAVSGARL
                     IRSGEAEVVLAGGTENMSQAPFVVRGVREGLPLGPGVLEDTLWAALTDSACGLGMAET
                     AENLAERYRISRDAQDEFAARSHARAARARATGRFAEEIVPVETPGGRGTPENVADDE
                     HIRPETTPERLRQLPARFREHGTVTAGNSSGINDGAAAVVLASERFAARSGLRPLGRL
                     RAWATVGVPPEIMGIGPAPASREALARAELALTAIGVVEVNEAFAAQYLAVEKELGLD
                     RERVNPNGGAIALGHPIGASGTRLLLTLLYELRHRGERFGLAALCIGGGQGIAAVVEA
                     A"
     gene            1408..2802
                     /locus_tag="VEW91_10485"
     CDS             1408..2802
                     /locus_tag="VEW91_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM92048.1"
                     /translation="MILTQYYLGCLAHASYLVADEASRTAAVIDPQRDVDQYIADAHQ
                     HGVEIRHVFLTHFHADFVAGHLELRARTGAQIYLGRQARAEYPFVPLGDGEEVAFGSV
                     RLRILETPGHSPESISILVYDLGRDPARPHAVLTGDTLFIGDVGRPDLRAALGWSAER
                     LAGMLYDSLREKLLTLPDETLVYPTHGAGSLCGRSIGKETVSTIGIQRRYNYALQPMS
                     KEAFIEIITADQPDAPAYFTFDAILNTKERPLLERSLEEGLRPLAIEELRGLQAARAQ
                     LLDARDPADFAGAHLLGSVNIGLGGQYATWAGTLLDRERPIVIVAGRGREGEAAMRLG
                     RIGFDHVAGYLGDGMEATELHRDLVRRLPRVTAPALAELLASADPPVVLDVRTTSERD
                     ADKIPGSLHIPLQHLLDRIAEVPQGPRTVVVYCATGYRSSIAASLLEQHGVPDVADLV
                     GGMAAWKASHLPTA"
     gene            2802..4262
                     /locus_tag="VEW91_10490"
     CDS             2802..4262
                     /locus_tag="VEW91_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYM92049.1"
                     /translation="MPLAEARIHLASRDALVRELREAVGAAYVLVDPGDLLAYEYDAS
                     NITAVPDLAVLPGSADEVARVLRIAARHGSPVVPRGAGTGVAGGALAIVGGVVVVMTR
                     MNRILEIDLASRVIVVEPGVTNVEISRAVAAEGYFYAPDPSSQYASSIGGNVAHNSGG
                     PHTIAYGVTTHHVLGVEVALPTGDLVRLGGIAPDAPGFDLTGLVVGSEGTFGIVTRIW
                     VRLLRRREGITTLLAIFAELDRASEAVTAIIGRGVGPVALEMLDRNTIQVVEPFVHAG
                     YPLDAEAVLLIEVEGIREALPRARAVVEAICRQYGAREIRIAHTEAERERLWLGRKAA
                     FGTMGRIAVNYYLHDAAVPRTRLPQVLRRVQEIGRREGLTLANVFHAGDGNLHPIIIF
                     DARIPGETERVIRAGEEMLRLCVEAGGTISGEHGIGFEKNNYMPWIYSAADLAAMRRV
                     KDVFDPAAIMNPWKLFPTPVSCAEVLVSSAPGVGRR"
     gene            complement(4177..4803)
                     /locus_tag="VEW91_10495"
     CDS             complement(4177..4803)
                     /locus_tag="VEW91_10495"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HYM92050.1"
                     /translation="MGLGRVTVFGSSQVTPGEAVYRDAVRLGRLLAERGYTVCSGGYT
                     GVMEAVSRGAAEASGTVIGITVEAWASRQQPNAWVAREVVTPHLFERLMRLVESDAYV
                     ALRGGPGTLGEVALVWNLFQTESIPLRPLILVGPEWRQLVRCFEAELRVESGDLALLQ
                     FAGTVEEVLPLLPGPPGRARPSVAPRRGQTRPAPPRRTPGSGTASRGS"
     gene            4938..5444
                     /locus_tag="VEW91_10500"
     CDS             4938..5444
                     /locus_tag="VEW91_10500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM92051.1"
                     /translation="MSPFVWVLIGVVLIAAAVYLSRGASAFTERWRVGRARSPGVRLD
                     RLDLTVPADPSVLRPIRQSLRRLASDLGLDRERSEALIVAVGEAVSNAIEHAYLVAPG
                     TIHVRARRSGAVLVVEVQDHGRWRQEGAGERRGHGLELMRAFADGVNIETTALGTIVR
                     LTLALPGR"
BASE COUNT          801 a         1917 c         2008 g          804 t
ORIGIN      
        1 cccgcttcgc cggggacgcc ccccaagagc tcctggaaca gtgctgcggc gtccgcgata
       61 cggcggacgg cgaccgcgac atggtcgaac cggatcatcc agcgacctcc tgcggggtat
      121 ctatcaagag atagcgggct acccggagtg tcattctcgc ctggaggagg agttccccgc
      181 ctctcacgga aaaacgggaa gtgccgggtt ggggttcggg agggaccgga tgaccgaggt
      241 cgtgatcctc gacggagcgc gtacgccgtt tgggcgcttt ggaggggagt tgcgcggggt
      301 gggggcgacg cagctgggga tcgcggcagc ccgcggcgca ctcgcccact cgaaggtcgc
      361 ccccgaggcc gtcgaccacg tcgtgtttgg caacgtcgtc cagtccagct cggacgccgt
      421 ctacctcgcg cggcacgtgg ggcttggggc cggcgtgcgg ctcgacagcc ccgccctgac
      481 ggtgaaccgc ctctgtgggt ccgggctcga ggccgcggtc tccggcgccc gcctgatccg
      541 gtcgggcgag gccgaggtgg tgctcgccgg cgggaccgag aatatgagcc aggccccgtt
      601 cgttgtacgg ggtgtgcgcg aggggttgcc cctcggcccc ggggtgctcg aggacacgct
      661 gtgggctgcc ctcaccgatt cggcctgcgg cctcgggatg gccgagaccg ccgagaacct
      721 ggcggagcgg taccggatct cccgcgacgc ccaggacgag ttcgcggccc gcagccacgc
      781 ccgcgccgcc cgcgcgcggg cgaccgggcg gttcgccgag gagatcgtcc cggtggagac
      841 ccccggtgga cgcgggacgc ccgagaacgt cgcagacgac gaacatatcc gtcccgagac
      901 cacgcccgag cggctccgcc agctgccggc gcgcttcagg gagcacggga cggtgaccgc
      961 gggcaacagc agcgggatca atgacggcgc cgcggcggtc gtgctcgcct ccgagcggtt
     1021 tgccgcgcgg agcgggctgc gccccctcgg tcgcctccgg gcgtgggcca cggtcggcgt
     1081 tccccccgag atcatgggca tcggccccgc cccggccagc cgcgaggccc tggcccgcgc
     1141 cgagctcgcg ctgaccgcga tcggcgtggt cgaggtgaac gaagcgtttg cggcccagta
     1201 cctcgcggtg gagaaggaac tggggctcga ccgcgagcgc gtcaatccca acggcggagc
     1261 gatcgcgctc ggccatccca tcggcgcgag cgggaccagg ctgctcctga ccctcctgta
     1321 cgaactgcgc caccgcggcg agcggttcgg tctcgcggcg ctgtgcatcg ggggagggca
     1381 ggggatcgcc gcggtcgtgg aggcggcatg atcctgaccc agtactatct cggttgtctc
     1441 gcccacgcgt cctatctcgt ggccgacgaa gcgagccgca ccgcggccgt aatcgacccg
     1501 cagcgggacg tggatcagta catcgcagac gcccatcagc acggggtgga gattcggcac
     1561 gtcttcctca cgcattttca cgccgacttc gtggccggcc atctcgagct gcgggcccgc
     1621 acgggcgcgc agatctacct cggccgccag gcccgggccg agtacccgtt cgtgccgctg
     1681 ggagacgggg aggaggtggc gttcgggtcg gtgcgcctca ggatcctgga gacccccggg
     1741 cactcccccg agtcgatctc gatcctcgtc tacgatctgg gccgggatcc cgcccgcccg
     1801 cacgccgtcc tgaccgggga cacgctcttt atcggggacg tcgggcgccc cgatcttcgg
     1861 gcggcgctgg ggtggtcggc ggagcgcctc gcgggcatgc tctacgactc cctccgggag
     1921 aaactcctca cgctgcccga cgagacgctg gtctatccga cccatggggc cgggtcgctc
     1981 tgcgggagga gcatcggcaa ggagaccgtg tcgacgatcg ggatccagcg gcggtacaat
     2041 tacgcgctcc agccgatgag caaggaggcg ttcatcgaga tcatcaccgc agaccagccc
     2101 gacgcccctg cgtacttcac gtttgacgcg atcctcaaca cgaaggagcg tcccctcctc
     2161 gagcgctcgc tcgaggaggg gctgcggccg ctcgccatcg aggagctgcg cgggctccag
     2221 gccgcccggg cgcaactgct cgatgcgcgg gacccggcgg atttcgccgg ggcgcacctt
     2281 ctgggcagcg tcaacatcgg gttggggggg cagtatgcga cctgggcggg caccctcctc
     2341 gaccgcgagc ggccgatcgt gatcgttgcc gggcggggga gggaggggga ggcggcgatg
     2401 cggctcggcc ggatcgggtt cgaccacgtc gccgggtatc tcggggacgg catggaagcg
     2461 acggagctgc accgtgacct cgtccgccga ttgccgcggg tgaccgcgcc cgcgctggcg
     2521 gagttgctcg cgtcagccga tcctccggtc gtgctcgatg tgcggaccac ctccgagcgc
     2581 gacgccgaca agatcccggg gagcctgcac atccccctcc aacacctcct ggaccgcatc
     2641 gcggaggtcc cgcagggccc gcgcacggtg gtggtctact gcgccaccgg ctaccggtca
     2701 tcgatcgccg cgagcctcct ggaacagcac ggggtcccgg acgtcgccga cctcgttggc
     2761 gggatggccg cctggaaggc ctcacacctc cccaccgcct gatgcccctc gcggaggccc
     2821 ggatccacct cgcctcccgc gatgccctcg tgcgcgagct gcgcgaggcg gtcggcgccg
     2881 cctacgtgct cgtggacccg ggcgatctgt tggcgtacga gtacgatgcc tccaacatca
     2941 ccgccgtccc cgacttggcc gtgctgccgg ggtcggccga cgaggtcgcc cgggtcctca
     3001 ggatcgcggc ccggcacggg agcccggtgg tgccccgcgg cgccggcacc ggggtcgccg
     3061 gcggagcgct cgcgatcgtg ggcggcgtgg tcgtcgtgat gacgcggatg aaccgcatcc
     3121 ttgagatcga cctcgcgagc cgcgtcatcg tggtggagcc gggggtgacc aacgtcgaga
     3181 tctcgcgggc cgtggccgcc gagggctact tctacgcccc cgatccgagc agccagtacg
     3241 cgagcagcat cgggggcaac gtggcccaca actcgggcgg tccgcacacg atcgcgtacg
     3301 gggtgacgac ccaccacgtg ctcggcgtgg aggtcgcgct cccgaccggg gacctggtgc
     3361 gcctgggggg gatcgccccc gacgcccccg ggttcgacct caccgggctg gtcgtggggt
     3421 cggaggggac gtttggcatc gtcacccgca tctgggtgcg gctgctccgc cggcgcgagg
     3481 ggatcaccac gctgctggcg atcttcgcgg aactggaccg ggccagcgag gccgtgaccg
     3541 cgatcatcgg gcggggcgtc ggaccggtcg cgctcgagat gctggaccgc aacacgatcc
     3601 aggtcgtgga gccgttcgtg cacgcggggt acccgctcga cgcggaggcc gtgttgctga
     3661 tcgaggtcga aggcatccgg gaggccctcc cgcgcgcccg cgcggtggtc gaggcgatct
     3721 gccgccagta cggtgcccgc gagattcgca tcgcgcacac cgaagcggag cgcgagaggc
     3781 tgtggctcgg ccggaaggcg gcgttcggca ccatggggcg gattgcggtg aactactacc
     3841 tccacgacgc cgcggtcccc cggacacggc ttccccaggt cctgcgccgc gtgcaggaga
     3901 ttggccgccg ggaggggctg accctggcca acgtgttcca cgcgggggac gggaacctgc
     3961 acccgatcat catcttcgac gcgcggatcc cgggggagac cgagcgggtc atccgggccg
     4021 gggaggagat gctgcggttg tgcgtcgagg ccggggggac gatctccggc gagcacggca
     4081 tcggtttcga gaaaaacaac tacatgccgt ggatctatag cgcggcggac ctcgccgcga
     4141 tgcgccgggt caaagacgtg ttcgaccctg ccgcgatcat gaacccctgg aagctgttcc
     4201 cgaccccggt gtcctgcgcg gaggtgctgg tctcgtctgc ccccggcgtg gggcgacgct
     4261 agggcgcgct cgtccgggag ggccggggag gagggggagg acctcctcga cggtgccggc
     4321 aaactgcagc agcgccagat cgcctgactc gactcgaagc tcggcctcga agcaccggac
     4381 caactgccgc cactcgggac caaccaggat gagcggccgc agcgggatcg attccgtctg
     4441 aaacaggttc cagaccagcg ccacttctcc gagcgtgccc gggcctcccc gaagggcgac
     4501 gtaggcgtcg ctctccacga ggcgcatcag gcgctcgaag aggtgcgggg tcacgacttc
     4561 cctggccacc caggcgttcg gctgctggcg ggaagcccac gcttcgacgg tgatcccgat
     4621 cacggtaccg ctggcctcgg cagcgccccg gctcaccgcc tccatcacgc cggtgtagcc
     4681 ccccgagcag accgtgtagc cgcgctcggc gagtaggcgg ccgaggcgca cggcatcccg
     4741 gtagacagcc tcgccggggg tcacctggga cgatccgaac acggttacac gacccagccc
     4801 catcgcggcc tcctcccaca ccaaggactt cgtacgaact tctcacggaa cgccggtggg
     4861 tcctccggcc cccggggcgt ttggcgcggg cccagggggg gtacaatagg cggcagtcgg
     4921 gaggggcgcg gaccgaggtg tcgccgttcg tctgggtgtt gatcggggta gtcctcatcg
     4981 ccgcggcggt atacctcagt cgcggcgcca gcgcctttac agaacgctgg cgcgtcgggc
     5041 gagcaaggtc ccccggtgtc cgcctcgacc gcctggatct gaccgttccc gcggatccct
     5101 ccgtcctcag accgatccgc cagtcgctca ggcgccttgc gagcgatctc gggctcgacc
     5161 gggagcggtc cgaggccctg atcgtggccg tcggcgaagc cgtgagcaac gcgatcgagc
     5221 acgcctatct ggtagcgccg ggaaccatac acgtccgggc ccgccgcagc ggcgcggtcc
     5281 tggtggtcga ggttcaagac cacggacggt ggcggcaaga gggggcaggg gagcgccggg
     5341 gtcacgggct cgagttgatg cgggcgttcg cggacggcgt gaatatcgag accacggccc
     5401 tcggcaccat agtccgcctg acgctggcgc tgcctgggcg ctagaggtat cggctacctt
     5461 tccctgtacg cctcacgacg atgagcaatg cgaagcacaa ggcacccgtc ttctgtccga
     5521 gcgtatatca
//
LOCUS       DASQGH010000536         5873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19347, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000536 DASQGH010000000
VERSION     DASQGH010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19347"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..534
                     /locus_tag="VEW91_10505"
     CDS             160..534
                     /locus_tag="VEW91_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HYM92052.1"
                     /translation="MYEFLTHHLIPHAQAEDRALYPTVAKIMGAPQATATMSRDHVEV
                     GRLTEELKLLRTRLSGAAVGEAEAKALRRVLYGLYAVVKVHFAKEEEVYLPILDAHLS
                     PQEAHRMFEAIEEAAKAAKSRR"
     gene            1281..1910
                     /gene="nthA"
                     /locus_tag="VEW91_10510"
     CDS             1281..1910
                     /gene="nthA"
                     /locus_tag="VEW91_10510"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HYM92053.1"
                     /translation="MTDANHDHGHQVVPSDLTLRVKALESLLVEKGLVDPAALDAVID
                     TYEHKVGPRNGAQVVARAWVDPAYKKRLLTDATAAIAELGYGGQQGEHMVVVENTPKV
                     HNIVVCTLCSCYPWSVLGLPPVWYKSAPYRSRAVIDPRGVLREFGLELPEDVEVRVWD
                     STAELRYLVLPERPAGSEKLSEKALAALVTRDAMVGVAKVTLSQAGGTA"
     gene            1907..2566
                     /gene="nthB"
                     /locus_tag="VEW91_10515"
     CDS             1907..2566
                     /gene="nthB"
                     /locus_tag="VEW91_10515"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008804891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HYM92054.1"
                     /translation="MNGVHDMGGMHGMGPIQYEKNEPVFHARWEGRAFALDLAMRAWR
                     KWNIDAHRHEIELIPAAEYLRMSYYEKWTASLVELMVKSGLITRAEVESGRPAPGLPK
                     ATPPLTAEKAPAMLRTGALARRNVSVTPRFQVGQRVRARNMHPTGHTRLPRYTRGKLG
                     TVERDHGVYVFPDTNAQFLGEKPQHVYSVRFAARELWGEQAAPRDAVYVDMWDDYLEP
                     A"
     gene            2529..2957
                     /locus_tag="VEW91_10520"
     CDS             2529..2957
                     /locus_tag="VEW91_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HYM92055.1"
                     /translation="MSTCGTTTLSQPDPIPETERLAALPRLPRDSEGPVFAEPWEAQA
                     FALAVKLSEQGHFTWKEWTAALADELKAAASRGEPDDGSRYYHYWLAALERLVTTKGL
                     TDPPALLARKEAWAEAYRRTPHGKPVELATGSVQHRVPRL"
     gene            3087..3383
                     /locus_tag="VEW91_10525"
     CDS             3087..3383
                     /locus_tag="VEW91_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HYM92056.1"
                     /translation="MVTPEEVRDTLRQVVDPELGANIVDLGLVYDIQVIGARVFVTMT
                     LTTPLCPMNEMIPDAVRQVAGVLPGVSGVDVELVWTPPWQPEMMSRQLKEMLGW"
     gene            complement(3611..3928)
                     /locus_tag="VEW91_10530"
     CDS             complement(3611..3928)
                     /locus_tag="VEW91_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92057.1"
                     /translation="MDINTASGQAAGIADEKFAHLSEYATSPHFTPLERKVLAFADGM
                     TISGHDTSDELFASLREHFSAAEMVELTAHVAFENFRSRFNHAFRIEAQGFCRLPRSS
                     GSP"
     gene            complement(3929..4219)
                     /locus_tag="VEW91_10535"
     CDS             complement(3929..4219)
                     /locus_tag="VEW91_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92058.1"
                     /translation="MATRRDAAPAHAGDHRVSRLAPPDPKGLPSAVQEILAEETQRFG
                     APLNTTTAWAHHPELLVAYKAWGKAMNAAPLIPAALKYLVYVRVASLNGCPF"
     gene            complement(4431..4799)
                     /locus_tag="VEW91_10540"
     CDS             complement(4431..4799)
                     /locus_tag="VEW91_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM92059.1"
                     /translation="MSAFVGSYRSAVAFRPERFNPVPIAQNERARVLLVCLEPEQFIP
                     VHRPGVDLTVVVLEGEGRLVVGDREEAIAPGAVAFAPAGTARGLKATTRLVVLNVVTP
                     PPTDADHAEVVAGLQRGRWR"
     gene            complement(4879..5142)
                     /locus_tag="VEW91_10545"
     CDS             complement(4879..5142)
                     /locus_tag="VEW91_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HYM92060.1"
                     /translation="MHGAPGGTGEGPGETALDVREIPPARRHPLIFQTFESLAPGQAF
                     TLINDHDPKPLFYQFQAERTGQFTWEYLEQGPDAWRVRIGRVT"
     gene            complement(5149..5598)
                     /locus_tag="VEW91_10550"
                     /pseudo
     CDS             complement(5149..5598)
                     /locus_tag="VEW91_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
BASE COUNT         1033 a         1881 c         1957 g         1002 t
ORIGIN      
        1 gagacacacg gatgagcatc agatcacaag aggcaggcac actcactcaa ctcctgcgcg
       61 atgagcacaa agaactgctg atgcgcttcg aggcgctgcg cacggtcgct gactcggtgg
      121 acgacgtatc ctccgaggaa gtgaagcgag gcgtgggagg tgtacgagtt cctgacccat
      181 cacctgatcc cccacgccca agcggaagac cgggccctct accctactgt cgcaaagatc
      241 atgggcgcgc cccaggccac ggccacgatg agccgtgacc acgtcgaggt gggccggctg
      301 accgaagagc tgaagttgct ccgtacgcgc ctgtcgggcg cagccgttgg cgaggccgag
      361 gccaaggccc tgcgccgcgt tctgtatggt ctttacgcgg tggtcaaggt gcacttcgca
      421 aaggaggaag aggtttacct ccccatcctg gacgcacacc tgagtccaca agaagcgcac
      481 cggatgttcg aggccatcga agaggcagcg aaagcggcga agagccgacg atagacgacc
      541 tgctgaggct aggctggaca tgatgttgcg atcgtcctag gctgggtcta gacgattggg
      601 aggtttatca tgccagcgga acagaaggtg cccgtgtatc ttcgcactca ccggcttcgc
      661 ggaaaggtgc tcaggtttac actcggtgca gaggatgaca tgctgcgcga gcaagctgcc
      721 tcctccaagt ccggccgcgc agccaaaact ctcatcaaac aaggaccgtt ccgcgtcacc
      781 cttgtggcca tgcggaaggg ggcagccctt caggagcacc acgtcgccgg ccccttgagc
      841 atacaggcga ttcgcggttg cgtgagggtc acgacgggcc aaggggacat tgacgttccg
      901 ccaggcgggc tgatcggact tgaatccggc gttgttcatt cggcgagagc gattgacgac
      961 tgcgcgatac tgatcacgtt agtcatgccg tgatcgggta caaatgggat gaatcttgag
     1021 ccccccgggg tatggtcaac cattcgaacg acgctgggac aatggcacct gcggctgacc
     1081 ctccagacga gaggttcggg gtggttgcct ggcacgtctc ggggttcagc caaaccgaag
     1141 ggggaatggc gaaggataag gggcaacaag agaaatggcg tctcgccccc cttcgcttga
     1201 caccgcacac cgcaagtcaa tattatcggc atgaagctct ggcattgccc gtaccgcgac
     1261 cggagcgggg gaaggggtgc ctgacggacg ctaaccatga ccacggacac caggtggttc
     1321 cttcggatct caccctgcgg gtcaaggcgc tcgaatcgct attggtcgaa aaggggctgg
     1381 tggatccggc agcgctcgat gcggtgatcg acacctacga gcacaaagtc ggcccgcgta
     1441 acggcgcgca ggtcgtggcg cgggcctggg tcgatcccgc ctacaagaag cgcctgctga
     1501 ccgacgccac cgcggcgatc gcggagctcg gctatggcgg acagcagggc gagcacatgg
     1561 tggtggtcga gaatacgccc aaggtgcaca acatagtcgt ctgcacgctg tgctcctgtt
     1621 atccttggtc ggtcctcggt ctgccgccgg tctggtacaa gtccgcgccc tatcgctcgc
     1681 gtgcggtgat cgatccgcgc ggggtgctgc gcgagttcgg gctcgagctg ccggaggatg
     1741 tcgaggtgcg cgtttgggac agcacagcgg agctgcgcta cctcgtgctg cccgagcggc
     1801 cggccggcag cgagaagctg agtgagaagg cgctcgccgc gctggtcacg cgagatgcga
     1861 tggtgggcgt cgcgaaggtc acgctgtcac aggcgggagg taccgcatga atggggtgca
     1921 tgacatgggc ggcatgcatg gcatgggccc gattcaatac gaaaagaatg aacccgtgtt
     1981 ccacgcgcgc tgggaggggc gggctttcgc gttggatctc gcgatgcggg cctggcgcaa
     2041 gtggaacatc gacgcccacc gccacgagat agagctaatc ccggcagccg aatacctgcg
     2101 catgagctac tacgagaaat ggactgccag tttggtcgag ctgatggtca agagtggcct
     2161 gatcacgcgc gcggaggtcg agagcggcag gccggcgccg ggcttgccca aggccacccc
     2221 gccgctcact gccgaaaagg cgcccgcgat gctccgtacc ggcgcacttg cgaggcggaa
     2281 cgtatcggtg accccgcgct tccaggtagg ccagcgcgtg cgcgcccgca acatgcatcc
     2341 gaccggtcac acgcgcctac ctcgctacac ccgcggcaag cttgggacgg tcgagcgtga
     2401 ccacggcgtc tacgtcttcc ccgacaccaa tgctcagttc ctcggcgaga agccacagca
     2461 cgtgtactcg gtgcgtttcg ccgcccgcga actgtggggt gagcaggctg caccgcgtga
     2521 tgcggtttat gtcgacatgt gggacgacta ccttgagcca gcctgatcca attccggaaa
     2581 ccgagcgcct cgcggcgctg ccgcgcctac cgcgcgactc ggaggggccg gtgtttgccg
     2641 agccgtggga ggcccaggcg ttcgcgctcg cggtcaagct ctccgagcag gggcatttca
     2701 cctggaagga atggacggct gcgctcgccg atgaactcaa ggcggccgcc agtcgcggtg
     2761 agcctgacga tggctcgcgg tactatcatt attggctggc ggctctcgag cgcctggtca
     2821 cgaccaaggg cttgaccgat ccgccggcgt tactcgcgcg caaggaggcc tgggccgaag
     2881 cttatcggcg cactccccac ggcaagcccg tcgagctggc caccggctcg gtgcagcaca
     2941 gagtaccccg gctataggtg gtgtcacgct cgacgagccc cggtgcgata tatgcaccgg
     3001 cctcacgcac agcgtgcccg cttccatctg tcgaggcgcg gacaccatta cgttccccaa
     3061 ccacccgtga cgacaggtga tggcccatgg tgacgccgga ggaggtacgg gacacgctcc
     3121 gccaggtggt cgacccggaa ctcggcgcga acatcgtcga ccttggcctc gtctacgaca
     3181 ttcaggtaat cggagccagg gtttttgtca cgatgacgct gaccacccct ttgtgcccga
     3241 tgaacgagat gatcccagac gccgtgcggc aggtggccgg tgtgctcccc ggcgtcagcg
     3301 gcgtcgatgt cgaactcgtt tggacacctc cctggcagcc ggagatgatg tcccggcagc
     3361 tcaaggagat gctggggtgg tgacccgcgg cggcgccggg ggccccggga tgcgtggaag
     3421 gcctccaggg tctccagtcg gggtctctga aagggagatg gtgaggagag taggggatcc
     3481 gatgaagcgg cccgacctga cggagctggt ggcccatgcc gcccggcggg cacggtgtcc
     3541 gttctgaagc cgatcccggt cgtgatccgc tgacagacac gcgttctcga gacgattatc
     3601 ccgccggaga tcagggagat ccgctcgatc gcggcagccg gcagaatccc tgggcctcga
     3661 tgcgaaacgc gtggttgaac ctgctccgga agttctcgaa cgcgacgtgc gcggtgagct
     3721 caaccatctc tgccgcggag aagtgctcgc ggagcgacgc aaacagctcg tcgctcgtgt
     3781 cgtggccgga gatcgtcatt ccatccgcga aagcgaggac cttccgctcg agcggagtga
     3841 agtgcgggct cgttgcatat tccgagaggt gcgcgaactt ctcgtccgcg attcccgccg
     3901 cctgccccga ggcggtgttg atgtccactc agaacgggca tccgttcagg gaggcgaccc
     3961 ggacgtacac gaggtatttc agggccgcgg ggatcagcgg ggccgcgttc atcgccttgc
     4021 cccaggcctt ataggcgacg agaagctcag gatgatgcgc ccacgccgtt gtcgtgttga
     4081 ggggcgcacc gaaacgctgg gtctcctccg caaggatctc ctgcaccgcg gaggggagac
     4141 ccttggggtc agggggcgcc agccggctta cccgatggtc tcctgcgtgg gccggtgcgg
     4201 cgtctcttct cgtagccatc tcggacctcc gttgttccgg gcctcggttt cttattctac
     4261 gctctgcgtt cgaactctgc cgtcgaggcc cctctgccgg cttccgcgtc acggcatgag
     4321 agagggaaac acgagtcagg atccggaggc ggtgccgcgc cctcggcggg cagatgttgt
     4381 ggtcttcgca tccgcccggc gcggggcgcg ggcggatcgt ttcatccgga tcatcgccag
     4441 cgcccgcgct gcagcccggc aacgacttcg gcgtggtctg catcggtcgg cggcggggtc
     4501 acgacgttga gaacgaccag ccgcgttgtg gctttgagcc cccgggccgt tcccgccggg
     4561 gcgaaagcga cggctcctgg cgcgatcgcc tcctcgcggt cacccacaac aagccttccc
     4621 tccccttcca gcacgacgac cgtcaggtcg acccccggcc ggtggaccgg gatgaactgc
     4681 tcaggttcca gacacacgag cagcaccctg gcccgctcgt tttgagcgat cgggacaggg
     4741 ttgaaccgct ccggccggaa ggccacagcc gatcggtagg aacccacgaa ggcactcatc
     4801 gacacccctc ctgtgtccac gcgtgtggca tggggtgatc cgccgagccg agaatcgtcc
     4861 gaggacactg ccccggggtc atgtcacccg gccgatccgc acgcgccatg cgtccggtcc
     4921 ctgctccagg tactcccacg tgaactggcc cgtccgctca gcctggaact ggtagaagag
     4981 cggcttggga tcgtggtcgt tgatcagcgt aaacgcttga cccggcgcca gggactcgaa
     5041 ggtctgaaaa atcagggggt ggcgccgggc cggtgggatc tcccggacgt ccagcgcggt
     5101 ctccccggga ccctcgcccg tcccacccgg cgcgccgtgc atccgttcca acacgcgctg
     5161 ctggtcctcg acggacacgt actgctcaaa cagggggagg tagacgcgct cctccttctc
     5221 caggtgcacc tggaataccg cctgcagctg aaggatgagg cgcgtgagct gagcgcgcag
     5281 cgccgggcga tcgcctggcg ctaccacccc cagccgcctc gcgctctggt cgatctggcg
     5341 gacgtaatct tcgatgaacc tgtgatcgat ccgcatcgtg gcggtcgcca tgccgtgggc
     5401 cttgagcagc gactccacgg cgggatacag gtgctgctcc tcgccggccg catgggtgag
     5461 cagctcgccc ttcaggaagg ccaccaagcc ttccgggtct gcgtcgggac ggccatcgac
     5521 gatcgcagct gcccgatcgg tgaattcctt cgtcagcgaa cggtggtggc gtcgaaaagc
     5581 ctcgcctgtc gtgctcatac gcttctcctc ctgcgctccc gggacgatgc cgctgatacg
     5641 cggtgccggc ccctggtcgt ttcttaggtg acagtggccg gatcccgggc cgcccgcggt
     5701 atctcggcag ggcgatggtg gaccagccgc accacgtgca ggatactcgc cacctcctac
     5761 ccgggcgtgc gagggcccag ccgggcccgc gcgacgcgct caccctcgct ctcgcctggc
     5821 tcggaggtga cctctaagcg gtccacccac tttacgctcg tgaagcaggc ccc
//
LOCUS       DASQGH010000537         4969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53248, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000537 DASQGH010000000
VERSION     DASQGH010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53248"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..850
                     /locus_tag="VEW91_10555"
     CDS             17..850
                     /locus_tag="VEW91_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYM92061.1"
                     /translation="MPDRASPGADAGPRPHRAGARVLTWLDAEYPARLRELRDAPPVL
                     YLRGVVASGPAVAVVGTRRPSAYGRDVTRSLGEVLGSAGVVVISGLARGIDAAAHAGA
                     LRPGGSTVGVLGCGVDIVYPPEHRTLMEAMLVRGGAATFQGRPVYAVPGSVFAPGSRG
                     PHRLLASGAAVLSDPGALLEGLGVRWPAPAGGRHAPRLGEDEARVLDLLGEAPVHIDG
                     LIERSGLGAGRVAGALATLEVRGLVRQSPGNRFARRSGFAGPGAQDQVGGDTWPNHSS
                     L"
     gene            826..2940
                     /gene="topA"
                     /locus_tag="VEW91_10560"
     CDS             826..2940
                     /gene="topA"
                     /locus_tag="VEW91_10560"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HYM92062.1"
                     /translation="MAKSLVVVESPTKARTVKKLLDRKYEVVASMGHVKDLPRSQLGV
                     DVEHEFTPKYVVPKGKGPVIKELKAAAKKASAVYLATDPDREGEAISWHLATILNPFN
                     AKIKRIEFHEVTRDAIRRALQAPRDIDTSLVNAQQARRILDRLVGYKLSPLLWRKVRG
                     GLSAGRVQSVAVRLICEREAEVEAFVPQEYWSITARLSREHEPTPFQARLVSKNGEKV
                     EIPNEAAARAIVEELERQPFQVSEIRRKDQHRHPAAPFTTSTLQQEANRKLGYSAART
                     MVVAQQLYEGLDVGAEGTVGLITYMRTDAVRVAEDAQREAREYITQAFGGPFVPAQPR
                     QYTSRRTAQGAHEAIRPTSVLRTPDAVKGFLKTDQYKVYRLIWERFVASQMASAVLDT
                     LAVDITAGAYILRATGSRVKFPGFLRVYHEGRDNGEEEAPEGWLPDVQQGERLRLLGL
                     DPAQHFTQPPPRYTEATLVRALEERGIGRPSTYAPIIETIKQRGYVEVEDRRMYPTEL
                     GVLVNSLLVEHFPTVVAVDLTAHLEEDLDRVEEGREDWVKLLRGFYGPFAEVLHKAEQ
                     NIEEVEISPEEIGEPCPRCGQPLVKRRGRFGQFIACSGYPSCSYTRPVGIGVRCPRDG
                     GEIVERRTRKGRMFYGCANYPACTFTSWDRPTGRMCPVCGQMLVFKRTRRGAPALVVC
                     GDKACKYQEAPKREVEQVGAPS"
     gene            2946..3500
                     /gene="hslV"
                     /locus_tag="VEW91_10565"
     CDS             2946..3500
                     /gene="hslV"
                     /locus_tag="VEW91_10565"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HYM92063.1"
                     /translation="MAGDDSEFRPLRSTTIIAVRREGAVAVAGDGQVTVGTTVLKHGA
                     RKVRRVGDGVLVGFAGSAADGLTLFEKLEQKLDAFHGNLPRAAVELAKDWRTDRVLRR
                     LDALMVVADREHLFVLSGSGDIVEPDDGVAAVGSGGPYALAAARALLQHSRLSAEEIA
                     REALRLTAEICVFTNNRVTVETLR"
     gene            3541..4932
                     /gene="hslU"
                     /locus_tag="VEW91_10570"
     CDS             3541..4932
                     /gene="hslU"
                     /locus_tag="VEW91_10570"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HYM92064.1"
                     /translation="MEALTPRRIVEELDKHIIGQGAAKRAVAIALRNRYRRSRIAPQE
                     LRDEVIPKNILMIGPTGIGKTEIARRLARLTAAPFVKVEATKFTEVGYVGRDVDSMVR
                     DLVEAAVQMVRAERLSTVDAPAAAHARERLLEILAPSPRREGAFANPLEALFGGRAAQ
                     PAVPSPEPPGDLLERRARLRERLERGELDQEMVEIEVEETGSPMIEVFSGQGAEEMGI
                     NLQDLVGNLLPRRRKRRRMTVPEALRVLTNEEAQKLIDPDEAVREGVRRAEESGIIFI
                     DELDKIAGHTGGAGPDVSREGVQRDILPILEGSTVTTKHGPVRTDHVLFIAAGAFHIA
                     KPSDLIPELQGRLPIRVELAPLTEDDFVRILVEPENALTRQYTALLATEGVRVAFTPD
                     GIGEIARIARQVNADTEDIGARRLHTILERVTEEISFAAPEAASAIVIDAPYVQRRLA
                     GILADRDLSRYIL"
BASE COUNT          775 a         1669 c         1808 g          717 t
ORIGIN      
        1 atccggacga gcgccggtgc ctgatcgcgc ttcacctggt gccgacgctg ggccccgtcc
       61 gcatcgcgcg ggggcgcggg tgctgacgtg gctggatgcc gagtatccgg cgcggctccg
      121 tgagctccgc gacgcgcccc cggtcttgta cctgcggggt gtggttgcat cggggcccgc
      181 ggtcgcggtc gttggaacgc gtcgcccgtc ggcgtacggg cgcgacgtga cccgctcact
      241 cggggaggtg ctcggaagtg ccggggttgt cgtcatcagc ggtctggccc gggggatcga
      301 tgcggccgcg cacgcaggtg cgctgcggcc gggcggaagc acggtggggg tgctggggtg
      361 cggcgtggat atcgtgtacc cgcccgagca ccgtacttta atggaagcga tgctcgtgcg
      421 cgggggtgct gccacttttc agggacgccc ggtctatgcc gtacccggca gtgtgttcgc
      481 cccggggagc cgcggcccgc ataggctgct ggcgagcggg gccgcagtcc tcagtgatcc
      541 cggggcgttg cttgagggac tgggggtgcg gtggccggcc cctgcgggcg gacggcatgc
      601 accgcgcctc ggagaagacg aggcgcgggt gttggacttg ctcggcgagg ctcccgtcca
      661 catcgacggg cttatcgagc gctcggggct gggggccggg cgcgtggcgg gcgcgctggc
      721 gacgctggag gtccgcgggc tggtccggca gtccccgggg aaccggttcg cacggcggtc
      781 cgggttcgca ggacccggcg cgcaagatca ggtaggaggc gacacgtggc caaatcactc
      841 gtcgttgtag aatcgccgac caaggcgaga accgtcaaga agctccttga ccggaaatat
      901 gaggtggtcg cctcgatggg gcacgtcaag gaccttcccc ggagccagct cggcgtggat
      961 gtcgagcacg agttcacccc gaagtacgtg gtccccaagg ggaaagggcc ggtcatcaag
     1021 gagttgaagg cggcggccaa aaaggcctcg gcggtctacc tggccaccga ccccgaccgg
     1081 gaaggcgaag cgatctcgtg gcacctggcg acgatcctca acccgttcaa cgccaagatc
     1141 aagcggatcg agtttcatga agtcacccgc gacgcgatcc ggcgggcgct ccaggctcct
     1201 cgggacatcg ataccagcct ggtgaacgcc cagcaggcgc gccggatcct ggaccggctc
     1261 gtcggctaca agctgagccc gctcctgtgg cgcaaggtgc gggggggact cagcgccggc
     1321 cgggtgcagt cggtggccgt ccggctgatc tgcgagcggg aagcggaggt cgaggccttc
     1381 gttccgcagg aatactggtc gatcacggcc cgcctgagcc gggaacacga acccaccccg
     1441 ttccaggccc gcctcgtgag caagaacggc gagaaggtgg agatcccgaa cgaagcggcc
     1501 gcccgtgcga tcgtggagga actggagcgg cagccgttcc aggtctcgga gatccgccgc
     1561 aaggaccagc accggcatcc cgcggcgccg tttaccacca gcacgctcca gcaggaggcc
     1621 aaccgcaagc tcggctactc ggccgcccgg accatggtcg tcgcgcagca actctacgag
     1681 ggcctggacg tcggggcaga gggcacggtc gggctgatca cctacatgcg gacggacgcc
     1741 gtccgggtcg cggaagacgc ccagcgcgag gcgcgcgagt acatcaccca ggcgttcggc
     1801 gggccctttg tgccggcgca gccgcgccag tacacctcgc gccgcaccgc gcagggggcg
     1861 cacgaggcca tccggcccac ctcggtgctc cgcacccccg acgccgtcaa ggggtttctc
     1921 aagacggacc aatacaaggt gtaccggctg atctgggagc ggttcgtggc cagccagatg
     1981 gcctcggcgg tgctcgacac cctggcggtc gacatcacgg ccggggcgta catcctccgg
     2041 gccaccgggt cgcgcgtgaa gttcccgggg tttctccgcg tctaccatga ggggcgcgac
     2101 aacggagagg aggaggctcc cgaaggctgg ctgcccgatg tccagcaggg tgagcgcctg
     2161 cgcctcctgg ggctcgatcc ggcccagcac ttcacgcagc cgccgccccg gtacaccgaa
     2221 gcgaccctgg tgcgcgcgct cgaagagcgg ggcatcggcc gtccgagcac ctatgcgccg
     2281 atcatcgaga cgatcaagca gcgggggtac gtcgaagtcg aggatcgtcg gatgtacccg
     2341 acggagctcg gcgtgctcgt caactctctc ctggtcgagc acttccccac cgtggtggcc
     2401 gtggacttaa ccgcccacct agaggaggac ctcgaccggg ttgaggaagg ccgggaggac
     2461 tgggtgaagc tcctccgggg attctacggg cccttcgccg aggtgctgca caaggccgag
     2521 cagaacattg aagaggtgga gatctcgccc gaggagatcg gcgagccgtg cccgcggtgc
     2581 ggccagcccc tcgtcaagcg acgggggcgg ttcggccagt ttatcgcctg ctccggatac
     2641 ccgtcgtgtt cgtatacccg gccggtcggg atcggcgtgc ggtgtccgcg cgacggcggg
     2701 gagatcgtcg agcggcgcac ccgaaaaggg cggatgttct acgggtgcgc caactacccg
     2761 gcgtgcacgt tcacgtcgtg ggaccgtccg accgggcgga tgtgcccggt gtgtggccag
     2821 atgctggtgt tcaagcggac ccggcggggc gccccggcgc tggtggtgtg cggcgacaag
     2881 gcatgcaagt accaggaggc ccccaagcgg gaagtcgagc aggtgggggc cccatcctga
     2941 cgagcatggc gggggacgac tccgagtttc gccccctgcg ctcgaccacg atcatcgcgg
     3001 tgcggcggga gggtgcggtc gcggtcgccg gagatggcca ggtgaccgtc gggaccacgg
     3061 tgctcaagca cggcgcgcgg aaggtgcgcc gggtcggcga cggggtgctg gtggggttcg
     3121 ccggatccgc cgccgacggc ctcacgctgt tcgagaaact cgagcagaag ctcgacgcgt
     3181 tccatggaaa cctcccccgg gcggcggtcg agctcgccaa ggactggcgg accgatcggg
     3241 tgctgcgccg gttggacgcc ctcatggtcg tggcggatcg ggagcacctg ttcgtgctct
     3301 cggggagcgg cgatatcgtc gagcccgatg atggcgtcgc ggcggttgga tcgggcggtc
     3361 cgtacgcgct ggccgccgcc cgcgcgttgt tgcaacactc ccgcctgtcc gccgaggaga
     3421 tcgctcgcga agcgctccgg ctgacggcgg agatctgcgt ctttaccaac aaccgcgtca
     3481 ccgtggagac cctccggtag ccggatgggc atggttcccc gcctgacccc tccgctcacg
     3541 atggaggcgc tcaccccccg gcggatcgtc gaggagctcg acaagcacat catcggtcag
     3601 ggggcggcca agcgggcggt cgcgatcgcg ctgcgcaacc gctaccgccg gagccgtatt
     3661 gcgccgcagg aactccggga cgaggtcatc cccaagaaca tcctcatgat cggcccgacg
     3721 ggcatcggca agacggagat cgcccggcgg ctcgcgcggc tcaccgcggc cccgttcgtc
     3781 aaagtggagg cgaccaagtt caccgaggtc ggatacgtcg ggcgggacgt cgactcgatg
     3841 gtgcgggacc tggtcgaggc cgcggtccaa atggtgcgcg ccgagcggtt gagcaccgtc
     3901 gacgcccccg cggccgccca cgcccgcgag cggctcctcg agatcctcgc cccctcgccg
     3961 cggcgggagg gggcctttgc caacccgctg gaagcgctgt tcggcggccg ggcggcccag
     4021 ccggctgtgc cctctcccga gcccccgggg gatctcctcg agcgccgcgc gcgcctgcgg
     4081 gagcgcctgg agcgggggga actggatcag gagatggtcg agatcgaggt ggaggagacc
     4141 ggctccccga tgatcgaggt cttctcgggc cagggcgccg aggaaatggg gatcaacctc
     4201 caggacctgg tcggcaacct cctgccccga cggcgcaagc ggcggaggat gacggtcccc
     4261 gaggctctgc gcgtgttgac gaacgaagaa gcccagaagc tcatcgaccc cgacgaggcc
     4321 gtccgcgaag gggtccgccg ggccgaggaa tcggggatca tcttcatcga cgagctcgac
     4381 aagatcgccg gccacaccgg cggcgccgga ccggacgtgt cccgggaggg cgtccagcgc
     4441 gacatcctcc cgatcctgga gggctccacg gtcaccacga agcacggccc cgtccgcacc
     4501 gatcacgtcc tgttcatcgc cgcgggcgcg ttccacatcg ccaagccgtc cgatctgatt
     4561 cccgagctcc agggccgcct ccccattcgc gtcgagctgg cgccgctgac cgaagacgac
     4621 ttcgtccgga tcctggtcga gcccgagaac gcgctcacca ggcagtacac cgcgctcctc
     4681 gctaccgaag gggtccgggt ggcgttcacg cccgatggca ttggcgagat cgccaggatc
     4741 gcccgccagg tcaacgcgga cacggaggac atcggggcgc gccgcctgca caccatcctc
     4801 gagcgggtga ccgaggagat ttcgttcgcc gctcccgaag ccgcctccgc catcgtgatc
     4861 gacgccccct acgtgcaacg ccggctcgcg gggatcctcg ccgaccgtga cctgagccgc
     4921 tacattctct gacgcctggt cgcctggacc gcgcccggcc gggaggctc
//
LOCUS       DASQGH010000538         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13882, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000538 DASQGH010000000
VERSION     DASQGH010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13882"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VEW91_10575"
     CDS             complement(<1..1057)
                     /locus_tag="VEW91_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYM92065.1"
                     /translation="MTNHWRDIKNADLILINGANPAEAHPVGFQWFMHAKLDPARGPG
                     HGGGAKIIHADPRFSRTSAVADMYLRIRAGADIAYFGGLINYVLENSHYHDEYVKHYT
                     NASFIVKDGYAFTDGLFSGYNKDKRSYDISTWAYESDQAATAAFERANEKALAHDGAH
                     ANFAKRDMTLQHPRCVFQLLKQHYARYTPEMVSSITGIPKEDFLKVAQLVGEMGRPDK
                     VMTIVYAVGLTHHTKGAQMIRSGAVLQLLLGNMGRPGGGMNAERGHANIQGNTDHAIS
                     WEILPGYLAIPAPGERTLDDYVREKAPKKLDPNSWNFFGTNYRKFMVSILKAWYGDAA
                     TNDNEFAFHYIPKPAGNS"
     gene            complement(1106..1690)
                     /locus_tag="VEW91_10580"
     CDS             complement(1106..1690)
                     /locus_tag="VEW91_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92066.1"
                     /translation="MATVTRRDFVKWSVAGAGTTAVSALGFDLARASEVRQQLRIAGA
                     TESHSICPYCAVGCAIIAFTKKNPDGSVQLLQIEGDPDSPVNEGRLCPKGATAMQLAT
                     SARRVESPLYRAPGTTEWKKVDWEFVLDKLAQNIKAARDRTFVTEDAQGNPVNRCEGI
                     AFAGGAAFSSEEGYFATKVMRGLGTVYIEQQARV"
BASE COUNT          289 a          640 c          628 g          375 t
ORIGIN      
        1 acgagttgcc ggccggcttg gggatatagt ggaaggcgaa ctcgttgtca ttggtcgcgg
       61 cgtcgccgta ccacgccttg aggatgctca ccatgaactt ccggtagttg gtgccgaaga
      121 agttccacga gttgggatcc agcttctttg gcgctttctc cctgacgtag tcgtccaggg
      181 tccgctcgcc cggggcgggg atggcaaggt agccggggag gatctcccag gagatcgcat
      241 ggtcggtgtt tccctggatg ttggcgtgcc cgcgctcggc gttcatcccc ccgcccggcc
      301 gtcccatgtt gccgagcagc aactggagca cggcgcccga ccggatcatc tgggcgccct
      361 tggtgtggtg ggtgagcccg accgcgtaga cgatcgtcat gaccttgtcg ggccggccca
      421 tctcgcccac caactgcgcg accttgagga agtcctcctt gggaatgccg gtgatcgagg
      481 acaccatctc gggcgtgtag cgcgcgtagt gctgcttgag gagctggaac acgcaccgcg
      541 gatgctgcag cgtcatgtcg cgcttggcga agttcgcgtg ggcgccgtcg tgcgccagcg
      601 ccttctcgtt ggcgcgctcg aacgccgcgg tggcggcctg gtcgctctcg tacgcccacg
      661 tcgagatgtc gtaggaccgt ttgtctttgt tgtagccgct gaacaacccg tcggtgaacg
      721 catacccgtc cttcacgatg aaggacgcgt tggtgtagtg tttgacgtac tcgtcgtggt
      781 agtggctatt ttcgagcacg tagttgatca gcccgccgaa ataggcgatg tccgcgcccg
      841 cccggatccg aaggtacatg tcggccacgg ccgacgtgcg ggagaaccgg ggatccgcgt
      901 gaatgatctt cgcgcccccg ccgtggcccg ggcccctcgc cgggtcgagc ttggcgtgca
      961 tgaaccactg gaacccgacc gggtgggctt cggccgggtt ggcgccgttg atcaggatca
     1021 ggtcagcgtt cttgatgtcc ctccagtgat ttgtcattgc cccgcgtccg aacgtggcgg
     1081 ccaaactgac caccgtgggg ccgtgtcaga cccgggcctg ctgttctatg tagaccgtcc
     1141 ccaacccgcg catcaccttg gtggcgaagt agccctcctc gctgctgaac gccgcaccgc
     1201 cggcaaacgc gatcccctcg cagcgattga cggggttccc ctgcgcgtcc tcggtgacaa
     1261 aggtccggtc ccgggcggcc ttgatgttct gggcgagctt gtcgagcacg aactcccagt
     1321 ccaccttctt ccactcggtc gtcccggggg cccggtagag cggtgactcg acgcggcggg
     1381 ccgatgtggc cagctgcatc gcggtcgcgc ccttcgggca gagccgcccc tcgttgaccg
     1441 ggctgtccgg atccccctcg atctgcagca gctgcaccga gccgtcgggg ttcttcttgg
     1501 tgaaggcaat aatcgcgcac ccgaccgcgc agtacgggca gatcgagtgg gactccgtgg
     1561 ccccggcaat ccgcaactgc tgccgcacct cggaggcccg cgccagatcg aaccccagcg
     1621 ccgagaccgc agtcgtcccc gcacccgcca ccgaccactt gacaaagtcc cggcgcgtca
     1681 ccgtggccat tggtttcctc ctcttgtatg tgagctactc ccaggcatcc atgcgggaac
     1741 gcacgagagg gcttcaagtt gtctcgtctg aggatgaaac ggggggaacg tcctgtcgga
     1801 atccactcag cggtcgagcc ggccccggcg cacgaaacgc gctccgcatc gcacgtcttg
     1861 gtggcccccg gaacagtgtg catcctgccc tcgtggcctg gccggcgtcc tgccgctcgc
     1921 cgtcagcatg gt
//
LOCUS       DASQGH010000539        10929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39269, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000539 DASQGH010000000
VERSION     DASQGH010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39269"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1210
                     /locus_tag="VEW91_10585"
     CDS             182..1210
                     /locus_tag="VEW91_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HYM92067.1"
                     /translation="MSISGQVIPGGNTLLRRIGPSLSRDARRRLRWMEFYLVHGRAVR
                     LTCRHFGISRATFYRWWQRYDPRRLQSLEDDRRTRRPRRVRQPQTPPVLVARIRAVRE
                     QFPRWGKVKLAVLLRRDGWPVSASTVGRTLTRLRAKGQLVEPAVVHVGRARWRRRRAR
                     PYAQRLPWGFVPHAPGDLVAIDATPVEVLPGRRRIHFTACDVVSRKDVLMAHLRLTSQ
                     AAEQMLRKAFGRFGFPIRAIQIDGGSEFKAAFETACQDLRIRLYVLPPRSPRLNGHVE
                     RAHRTHQEEFYDLQEIPEALGAHNMVLRDWEDIYNSVRPHQALGYLTPNEFIARWQAT
                     HGHERRRV"
     gene            1343..1633
                     /gene="gatC"
                     /locus_tag="VEW91_10590"
     CDS             1343..1633
                     /gene="gatC"
                     /locus_tag="VEW91_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYM92068.1"
                     /translation="MIDDRTVAHVARLSRLELSDEERRQFREQLGHILEHFQTLNALN
                     LDGVPPTAHARATANVLRDDVVRPSLDPAATLANAPAAEDGYFVVPPVILED"
     gene            1682..3109
                     /gene="gatA"
                     /locus_tag="VEW91_10595"
     CDS             1682..3109
                     /gene="gatA"
                     /locus_tag="VEW91_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HYM92069.1"
                     /translation="MYRRGETRPSEVVRVLLDRIEAHEARLHAYLFLDRDAARQEAAR
                     WDARVRDADAPPLAGIPIAVKDVICTRGWPTTCASRILDGFRPPYDATAVTRLREAGA
                     ILMGKTNCDEFAMGSSTENSGYGVTRNPWDDARVPGGSSGGSAAAVAAGEAAAALGTD
                     TGGSIRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPLTHDVRDAALLLQVIAG
                     HDPADSTSAVLDVPPYPDRLTGDIRGVRLGVVREFLADGVDPGVQAAVRAAVKVYSDL
                     GAACEEVSLPHAPYALPAYYLVAPAEASSNLARYAGVHYGHRTRDAGDLITMYSRSRR
                     EGFGAEVKRRIMLGTYALSAGYYEAFYLRAQRARTLIRQDFDAALARCDALVGPVSPT
                     AAFRIGEKVDDPLQLYLSDVYTVPINLAGVPGISIPCGLAGGLPVGLQVIGRPFGEET
                     VLNVAYAFEQATAFHRLRPALAPPS"
     gene            3116..4552
                     /gene="gatB"
                     /locus_tag="VEW91_10600"
     CDS             3116..4552
                     /gene="gatB"
                     /locus_tag="VEW91_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYM92070.1"
                     /translation="MGYETVIGLEVHVQLLTASKMFCGCAVAFGAPPNTLTCPVCLGL
                     PGSLPVLNGRAVELGIRTALALGCRIQPESQFHRKNYYYPDLPKNYQISQYEYKGHPP
                     LATGGVLRVPAGTAIREIGIQRVHLEEDTGKLVHAPGRSLVDYNRCGTPLMEVVTEPD
                     LRSPEEARLFLTHLRAALQFAEVTSGRLEEGTFRCDANLSLRPPGGAHGTRTEVKNMN
                     SLRAVERALRFEEARQREVLERGERVVQETRHWDEDRQITFASREKEEAQDYRYFPEP
                     DLTPLQIDPAWVDRIRAGLPELPEARRRRYVETLGLPEHDAAVLTSTRAMAEFFERTV
                     AAFQTPKVVSNWLIGDVAAYLNAQGREIEEVPIRPEHLAGLLALIEAGQLSGRSAKEV
                     LEEMLRTGRPAEAIVRERGLVQISDEAMLAAVVQEVIAEHPGPVGDVRAGKDQAVTFL
                     VGQVMKKTRGRANPGMVNRLIRERRRVG"
     gene            4542..5954
                     /gene="rlmD"
                     /locus_tag="VEW91_10605"
     CDS             4542..5954
                     /gene="rlmD"
                     /locus_tag="VEW91_10605"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HYM92071.1"
                     /translation="MSDSAARQRLARPVRLRTTRRPRPRPAAGRLAPGREVTARFHRM
                     GPTGEALATIGGREVAVPYAAPGEEARIRILSLEPRRARGRLVALRVASPRVVQPRCP
                     HFGRCGGCQWQHLEYPAQLEQKTALVSAALARGRIQGPPIAPAIGWEPSWEFRAHLEA
                     AVGLRDGQPVLGFYSWGGDRIVEARECPVQHPGNVAALNSVRAAWEALHPLGGILRGL
                     ISRVGTATGEVMVGLSVPRRLRPEEREIAVRALLDAVPGLVSLMEVRAPRRGHLLAGR
                     RADLLWGRPYVQEEVAGVRFHVPLLAEFPVNLRALPGLIEIILGALDATPQDTVVEPD
                     AGIGGYTLHVALGAARVLAVTTAAQLDAAWANARLNAVTNCLFYARSPVRALEKVRRR
                     APVRLAFLHPPGTGLASDLPQALRQAGVERVVYLGRTLRGLAPDAVALERAGFRIRQV
                     QPIDLSPHTSRVHALITASV"
     gene            5969..6313
                     /locus_tag="VEW91_10610"
     CDS             5969..6313
                     /locus_tag="VEW91_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92072.1"
                     /translation="MEATMPLVTAFNLRKHDQLSEIEKALKDALVSIPELKINDYEID
                     LVPVFTPDGFHGTVTRINVDLWERTERTKEALQELATRVAKAFGNVAGRDRKVKVVIR
                     PYDVEKSGWVSF"
     gene            6592..10068
                     /locus_tag="VEW91_10615"
     CDS             6592..10068
                     /locus_tag="VEW91_10615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYM92073.1"
                     /translation="MPAEFVHCHLHTEYSLLDGESRIGPLMSRAAALGMRAVALTDHG
                     ALYGAVEFYEQARVHGLTPIIGVEAYVAPRRMGDKDPKLDAPAFHLVLLAENAEGYRN
                     LLALTTAAHLDGFYYKPRIDRELLRRHAGGLIGLSGCLQGEVPRALLRGDAKAAREIA
                     ATYRDILGRGNFYLEVQDHGLPDQRTVTQGLVPIARDLTLPIVATNDVHYVTRDEADA
                     QDVLMCIQMGLALDQKDKPRMGETPEFYLKSPDEMAARFRDLPEALENTVAIAERCRL
                     EIETGGVNLPHFPVPEGETPDSYLRALCEAGLRRLYGEVTPALRERLDYELGVIARMG
                     FAAYFLIVQDFVNFARRRGILTTVRGSAAGSLVLYACSVTDVDPLVYRLPFDRFLNLV
                     RYTMPDIDVDFMDSRRDEVIAYVMDKYGADRVAQIITFGTMGARAAIRDVGRVMGLPY
                     GEVDRTAKLIPGPTVTLREAIDAEPELRAAADGNPQVRRLLDLAQKLEGVARHASTHA
                     AGVIISRDPLITQVPLQRATKGDLVMTQYDMNSVAAIGLLKMDFLGLTNLTILDTAMR
                     IIRETREVVIDLARIPLDDPPTYALLASGETTGIFQLEGRGMRRYLKDLRPDRIEDVM
                     AMVALFRPGPMANIPAYIRRKHGEEPVTYLHPLLEPVLKDTYGVMVYQEDIMTVAQAI
                     AGYTMAEADVLCYAIRKKIKDRLLAQREQFVAGARRNGVPPRIVDQIFEQFEPFARYG
                     FNRAHAACYGLIAYYTAYLKAHFPAEYMTAVMTSDAGNTEKVAQSVAECHRMGIRILP
                     PDVNRSAASFTVEGDAIRFGLAAVRNVGTTAVDSMIAARTSGGPFTGLADFCARVDTR
                     QVNQRVVASLVKAGALDAPGEPRARMLQTLDDTMEQAQRAQRVRAQGQTGLFDMGSEA
                     PPLADASGDEFSPEELLTMEKEMLGLYISDHPLHRWQPVLAQRVTAQLAHLPDLPDRK
                     EVVVGGLVSGMKRTLTRSGSVMAFLTLEDLTGSVEVVVFPRVYEQQASALKRDAVLLL
                     RGKVDIQEQSAKFLCEEVLPLPSSPDPAAPDGDPARAASPPPGRPADGGEDSEDPARP
                     PLRVRVSTREEVAQLERYLLDHPGDRQVCVHVVTDQGEEHIVPARVRLHDADGLHQEL
                     EQIFGEGNVWEE"
     gene            10143..10640
                     /locus_tag="VEW91_10620"
     CDS             10143..10640
                     /locus_tag="VEW91_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HYM92074.1"
                     /translation="MTPGVALAKTAQEIDAGVNAALDRFTSQVKGSKELLQRAKGVLV
                     FAGVLQAGFVYGGEYGEGALRLGGKTRTYYNIAPASFGLQLGAQKKDIILVFLQDKAL
                     KDFQAKSGWQIGVDASVVLVDVGAQGSIDSTKLNQPIVGFVVGQSGLMYNLSLSGSKI
                     SRLEK"
     gene            complement(10657..>10929)
                     /locus_tag="VEW91_10625"
     CDS             complement(10657..>10929)
                     /locus_tag="VEW91_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HYM92075.1"
                     /translation="DCAAMQGARVEFWLRAPDGQYDDVHRGTVITDGNGRYRFESNFP
                     GGGGGFFQPHIHLRVAVPGYKALVTIYLPQAGATTGTFDLVLEPEV"
BASE COUNT         1697 a         3788 c         3812 g         1632 t
ORIGIN      
        1 ccgccgccgc ggccgtcttg ctgtatcgag ccgcacatcc gtccggccag ggtgaggcgt
       61 aggcgcatcg ctcgcgcagt ggagcgttgt gcaggttcag atagatccga ctacacctcg
      121 gtgagtgccg cgacggaaag ccgggtttcg gctgcatgtg aagggccgat atactggccc
      181 catgagcatc agtggccaag tcattcccgg cgggaatacc ttgctgcgac ggattgggcc
      241 ctcgctctcc cgggacgccc gccggcgctt acggtggatg gagttctatc tggtccacgg
      301 gcgcgcggtc cggctgacct gccggcactt tgggattagc cgcgccacgt tttatcgctg
      361 gtggcagcgc tatgatcccc gccgcctcca gagcctcgaa gatgaccggc ggacccggcg
      421 cccgcgccgt gtgcgccagc cccagacgcc ccccgtgctg gtcgcccgga tccgggccgt
      481 gcgggaacaa tttccccggt ggggcaaggt gaaactcgcc gtgctcctgc ggcgggacgg
      541 ctggccggtg tcggcctcca ccgtggggcg gaccctcacc cgcttgcgcg ccaagggcca
      601 gctcgtcgaa ccggcggtcg tgcacgtagg ccgggctcgc tggcggcggc gccgcgcccg
      661 gccgtatgcg cagcggctgc catggggctt cgtcccgcac gccccgggcg acctcgtcgc
      721 gatcgatgcc acgcccgtgg aggtgctccc gggccggcgg cggatccact tcacggcctg
      781 cgacgtggtg agccggaagg atgtgctgat ggcgcacctg cggctgacca gccaggcggc
      841 cgagcagatg ttgcggaagg cgtttggccg atttggcttc ccgatccgcg ccatccagat
      901 cgatgggggc tccgagttca aggcggcgtt tgagaccgcg tgccaggacc tgcggattcg
      961 gctctatgtc ctcccaccgc gcagcccccg cctgaacggg catgtcgaac gcgcccaccg
     1021 gactcaccag gaagagtttt acgacctcca agagatccca gaggctctgg gagcgcataa
     1081 catggtgctc cgggattggg aagacatcta caatagcgtc cggccccacc aggccctggg
     1141 ctatctcacc ccgaacgagt tcatcgctcg gtggcaggct acgcatggcc acgaaaggag
     1201 gcgtgtgtag tcggatctac tggaccagta cagacgcttg agccgccccg ccggcctatg
     1261 ctacgatggc agcattcgtt gccccccatg cccccatccg ggggacaggc ggggggcagg
     1321 tagcccaggg gggtccggat ccatgatcga tgaccgcacc gtcgcccacg tcgcccggct
     1381 cagccggctc gaactctccg acgaggaacg gcggcagttt cgcgagcagc tcggccacat
     1441 cctcgagcac ttccagacgc tcaacgcgct gaacctcgac ggggtccccc ctacggcgca
     1501 cgcccgcgcc acggccaatg tcctgcgcga cgacgtcgtc cggccgtccc tcgatccggc
     1561 cgccaccctg gccaacgcgc ctgccgccga ggacgggtac ttcgtcgtgc ctccggtcat
     1621 tctggaggac tagcccaggg tgctccccct cgccgatcgg ccgatctggg aactccgcgg
     1681 gatgtaccgc cgcggggaga cccgtccgtc cgaggtcgtc cgggtcctcc tcgaccggat
     1741 cgaggcgcat gaagcgcgcc tccacgccta cctgtttctc gatcgggacg ccgcgcgcca
     1801 ggaggccgcg cggtgggatg cgcgggtgcg ggacgcggac gcgcccccgc tggccgggat
     1861 cccgatcgcc gtgaaggacg tcatctgcac ccgggggtgg cccacgacct gcgcctcccg
     1921 gatcctcgac ggcttccggc cgccctacga tgcgaccgcg gtcacgcgcc tgcgggaggc
     1981 cggggcgatc ctgatgggca agacgaactg tgatgagttc gccatgggca gcagcaccga
     2041 gaactccggc tatggggtga cccggaaccc gtgggacgac gcccgggtgc cgggggggag
     2101 cagcggcgga tcggccgcgg ccgtggcggc gggcgaggcc gcggccgcgc tggggacgga
     2161 tacggggggc agcatccgcc agccggcggc gttttgcggc gtggtcggcc tgaagcccac
     2221 ctacggccgg gtctcgcgct acggcctcgt ggcgttcgcc tcctcgctcg atcagatcgg
     2281 gccgctgacc cacgatgtcc gcgacgccgc gctgctgctg caggtcatcg ccggtcacga
     2341 cccggccgac tccacctcgg ccgtcctcga cgtcccgccc tatccggacc ggctgaccgg
     2401 ggacatccgc ggggtgcgcc tgggggtcgt gcgggagttc ctcgcggatg gcgtggaccc
     2461 gggggtccag gcggcggtgc gcgcggcggt caaggtttat agcgacctgg gcgcggcctg
     2521 cgaagaggtg tccttgcccc acgcgccgta cgcgctcccg gcctactatc tggtcgcgcc
     2581 cgcggaagcg agcagcaacc tggcccggta cgccggcgtc cactacgggc accggacccg
     2641 ggacgcgggg gatctgatca cgatgtattc ccgctcccgg cgggaaggct tcggcgccga
     2701 ggtgaagcgc cggatcatgc tgggcacgta cgccctgtcc gccggctact acgaggcctt
     2761 ctatctccgc gcccagcgcg cccggaccct gatccgccag gacttcgacg ccgccctggc
     2821 ccgctgcgac gccctcgtgg gcccggtctc ccccaccgcc gcgtttcgca tcggggagaa
     2881 agtggacgat ccgctgcagc tctacctctc cgacgtctac accgtgccga tcaacctggc
     2941 cggcgtcccg gggatctcga tcccctgcgg gctggccggc ggccttcccg tggggctgca
     3001 ggtcatcggc cgccccttcg gcgaggagac ggtgctcaac gtcgcctacg cgttcgagca
     3061 ggccacggcg ttccaccggc tgcggccggc gctggcgccg ccctcctgat cccgcatggg
     3121 ctacgagacg gtcatcgggt tggaagtcca cgtgcagctc ctgaccgcca gcaagatgtt
     3181 ctgcggatgc gccgtcgcgt tcggcgctcc cccgaacacg ctgacctgcc cggtgtgcct
     3241 ggggctgccg ggctcgctgc ccgtcctcaa cggccgggcc gtcgaactgg ggatccgcac
     3301 cgcgctggcc ctcggctgcc gcatccagcc ggagagccag tttcaccgga agaactacta
     3361 ttacccggac ctcccgaaga actatcagat ctcccagtac gagtacaagg gccatccgcc
     3421 gctcgcgacc ggcggcgtgc tccgcgtgcc ggccgggacg gcgatccggg agatcggcat
     3481 ccagcgggtg cacctcgagg aggataccgg gaagctggtc cacgccccgg gccgcagcct
     3541 ggtcgactac aaccggtgcg gcacgccgct gatggaggtg gtgaccgagc ccgatctgcg
     3601 ctcgcccgag gaggcccggc tgtttctgac ccacctccgc gccgcgctcc agttcgcgga
     3661 ggtcacgagc gggcggctgg aggagggcac gttccggtgc gacgccaacc tctcgctgcg
     3721 cccgccgggc ggggcgcacg ggacccggac cgaggtcaag aacatgaact ccctccgcgc
     3781 ggtagagcgc gccctgcgct tcgaggaggc gcggcagcgc gaggtcctgg agcgcgggga
     3841 gcgggtggtc caggagacgc ggcactggga cgaggaccgg cagatcacct tcgcctcgcg
     3901 cgagaaagag gaagcccagg actatcgcta cttcccggag ccggatctca cgccgctcca
     3961 aatcgatccg gcgtgggtgg accgcatccg cgcgggcctg ccggagctgc cggaggcgcg
     4021 ccgccgccgg tacgtggaga cgctcggcct ccccgagcac gacgcggcgg tgctgacctc
     4081 tacgcgcgcg atggcggagt tctttgaacg gaccgtcgcg gcgtttcaga cgccgaaggt
     4141 ggtcagcaac tggctcatcg gcgacgtggc cgcctacctc aacgcccagg ggcgcgagat
     4201 cgaggaggtg ccgatccggc cggagcacct cgccggcctc ctcgccctca tcgaggcggg
     4261 acaactgagc gggcggtcgg cgaaagaggt gctcgaggag atgctccgga ccgggcgtcc
     4321 cgcggaggcg atcgtccggg agcgcgggct ggtgcagatc agcgatgaag cgatgctggc
     4381 ggcggtggtc caggaggtca ttgccgagca ccccgggccg gtgggcgacg tgcgcgcggg
     4441 aaaggatcag gcggtcacct ttctcgtggg ccaggtgatg aagaagaccc gcggccgggc
     4501 caacccgggg atggtcaacc gcctgatccg ggagcgccgg cgtgtcggat agcgcggccc
     4561 gccagcggct cgccaggccc gtccgcctgc gcactacccg gcggccgcgg ccgcgaccgg
     4621 ccgccgggcg cctcgccccg gggcgcgagg tgaccgcccg gttccaccgg atggggccca
     4681 ccggcgaggc gctcgcgacg atcggcgggc gcgaggtggc cgtgccctac gccgcgccgg
     4741 gggaagaagc ccgcatccgc atcctcagcc tggagccccg ccgggcgcgg ggccggctgg
     4801 tggccctccg ggtcgcctcc ccgcgggtcg tccaaccccg ctgcccgcat ttcggccggt
     4861 gcgggggatg ccagtggcag cacctcgagt acccggcgca actcgagcag aagacggcgc
     4921 tcgtttccgc ggccctcgcg cgcggccgca tccagggccc gcccatcgcc ccggcgatcg
     4981 gctgggagcc gtcctgggag ttccgcgcgc acctcgaggc cgcggtcggc ctacgcgacg
     5041 ggcagccggt gctggggttc tacagttggg gcggggaccg catcgtcgaa gcgagggagt
     5101 gtccggtcca gcacccgggg aatgtcgcgg cgctgaacag cgtgcgggcg gcgtgggagg
     5161 cgctccaccc gctggggggg atcctccgcg ggctcatcag ccgggtcggc accgccaccg
     5221 gtgaggtcat ggtcggcctg tcggtccccc ggcgcctgcg cccggaggag cgcgagatcg
     5281 ccgtccgggc gctgctcgac gccgtgccgg gtctcgtgag cctgatggag gtccgcgccc
     5341 cccgccgcgg gcacctcctc gccggccggc gcgcggacct tttgtggggc cggccgtatg
     5401 tgcaggagga ggtcgcgggg gtccggttcc atgtcccgct gctggccgag ttcccggtga
     5461 acctccgcgc cctcccgggg ttgatcgaga tcatcctcgg cgcgctcgac gcgacccccc
     5521 aggacaccgt cgtggaaccg gatgccggga tcggaggcta caccctgcac gtggcgctgg
     5581 gcgcggcgcg cgtactggcg gtgacgacgg ccgcccaact cgacgctgcc tgggcgaacg
     5641 cccgcctcaa cgcggtcacg aactgcctct tctacgcccg gagccccgtg cgcgcgctcg
     5701 agaaggtccg gcgccgggcg ccggtccgcc tggcgttcct gcatcccccc gggacagggc
     5761 tggcgtccga cctcccccag gccctccggc aagccggggt cgaacgcgtg gtgtacctcg
     5821 gccggacgct ccgggggctc gccccggacg cggtcgcgct cgagcgggcg ggattccgca
     5881 tccgccaggt gcagccgatc gacctcagcc cgcacacgag ccgcgtgcac gccctgatca
     5941 cggcctcggt ctgatgctcg aggtatgaat ggaggcaacc atgccactcg tcacggcgtt
     6001 caatttaaga aaacacgatc agctgtcaga aattgaaaaa gccttaaaag atgccttggt
     6061 gagcataccc gaactaaaaa tcaatgacta cgagattgat cttgttcctg tctttacccc
     6121 cgatggtttc catggaacag taaccagaat taatgttgat ctatgggaaa gaaccgagcg
     6181 cacaaaagag gctctgcaag agcttgcaac aagagtcgcc aaagcctttg gaaatgttgc
     6241 tggcagagat agaaaggtca aagtcgtcat caggccttat gatgttgaaa agagtggttg
     6301 ggtatccttc tgatccattt ttgttttttg cacatgtgac cccaaaggaa gatttggggt
     6361 tgatgtgctc gattgcaatg cggggcaaga tgaggtccgc tatctcccgg tagttgagag
     6421 aaagcaaaat gaaaataaat gcaaaagcac caatttgtcc ccgcccgagg gaatggtggt
     6481 ataggcctgg gagaggaaga gggccgccgg atgtggtaac ttgaagggga gccccaagcg
     6541 gcgttggccc cgtgatcccg cagctgctcc ggttccggag gacgtacctg aatgcccgct
     6601 gagttcgtcc actgccacct ccacaccgaa tactcgttgc tcgacggtga aagccgcatc
     6661 ggcccgctca tgtcgcgcgc cgccgcgttg gggatgcgcg ccgtggcgct caccgaccac
     6721 ggggcgctgt acggggcggt cgagttctac gagcaggccc gcgtccacgg cctgaccccc
     6781 atcatcggcg tcgaagccta cgtcgcgccc cgccggatgg gggacaagga tcccaagctc
     6841 gacgccccgg cgttccatct cgtcctcctc gcggagaacg ccgagggcta ccggaacctc
     6901 ctggcgctca cgacggccgc ccacctcgac gggttctact acaagccgcg gatcgatcgc
     6961 gagctgctcc gccggcacgc cggggggttg atcgggttgt cggggtgcct ccagggcgag
     7021 gtgccgcggg ccctgctgcg cggggacgcc aaagcggcgc gcgagatcgc cgcgacctac
     7081 cgcgacatcc tcgggcgggg gaacttctac ctggaggtcc aggaccacgg gctccccgat
     7141 cagcgcaccg tcacccaggg cctcgtgccg atcgcccggg acctcacgct cccgatcgtg
     7201 gcgaccaacg acgtccacta tgtcacccgg gacgaggcgg acgcccagga cgtcctgatg
     7261 tgcatccaga tggggctggc gctcgatcag aaggacaagc cccggatggg cgagacgccc
     7321 gagttctacc tcaagagccc cgacgagatg gcggcgcgct tccgcgacct gcccgaggcc
     7381 ctggagaaca cggtcgcgat cgccgagcgg tgccggctcg agatcgagac cggcggggtc
     7441 aacctcccgc acttccccgt gccggagggg gagacgccgg actcctatct ccgcgcgctg
     7501 tgcgaggccg ggctgcgccg cctgtacggg gaggtcacgc cggccctccg cgagcgattg
     7561 gactacgagc tcggcgtcat tgcccggatg gggttcgccg cgtacttcct gatcgtccaa
     7621 gacttcgtga acttcgcgcg ccggcgcggc atcctgacca ccgtccgcgg gtcggccgcc
     7681 gggagcctcg tgctgtacgc ctgcagcgtg accgacgtgg acccgctggt ataccgcctg
     7741 ccgttcgacc ggttcctcaa cctcgtgcgc tacacgatgc ccgatatcga cgtggatttc
     7801 atggacagcc gccgggacga ggtcatcgcg tacgtgatgg acaagtatgg cgcggaccgg
     7861 gtcgcgcaga tcatcacatt cgggacgatg ggcgcgcgcg cggcgatccg cgacgtcggc
     7921 cgggtgatgg gcctccccta cggggaggtc gaccggaccg cgaagctcat ccccggcccc
     7981 accgtgacgc tcagggaagc gatcgacgcc gagcccgagc tgcgggccgc cgcggacggg
     8041 aacccccagg tccgccgcct cctggatctc gcccagaagc tcgaaggggt cgcccggcac
     8101 gccagcacgc acgcggcggg cgtgatcatc tcccgcgacc ccctgatcac ccaggtcccg
     8161 ctccagcggg ccaccaaggg cgacctcgtg atgacccagt acgacatgaa cagcgtcgcc
     8221 gcgatcggcc tgttgaagat ggacttcctc ggcctcacga acctcacgat cctggacacg
     8281 gcgatgcgca tcatccggga gacccgggag gtggtgatcg acctcgcccg catcccgctc
     8341 gatgacccgc cgacctacgc gctcctcgcc tccggcgaga cgaccgggat cttccagctg
     8401 gagggccggg gcatgcgccg gtacctcaag gacctgcggc ccgaccggat cgaggacgtc
     8461 atggcgatgg tggcgctctt ccgccccggg ccgatggcga acatcccggc ctacatccgc
     8521 cgcaagcacg gggaggagcc ggtcacctac ctccacccgc tgctcgagcc ggtcctcaag
     8581 gacacctacg gcgtcatggt gtaccaggag gacatcatga ccgtggccca ggccatcgcg
     8641 ggctacacga tggccgaggc ggacgtcctg tgctacgcga tccggaagaa gatcaaggat
     8701 cgcctgctcg cccagcggga gcagttcgtg gccggggccc gccggaacgg ggtcccgccg
     8761 cgcatcgtcg accagatctt cgagcagttc gagccgtttg cccggtacgg gttcaaccgg
     8821 gcgcacgcgg cctgttacgg gctcatcgcc tactacacgg cgtacctcaa ggcccatttc
     8881 ccggccgagt acatgaccgc ggtgatgacg agcgacgcgg gcaacacgga gaaggtggcg
     8941 cagtccgtgg ccgagtgcca ccggatgggc atccggatcc tgccgccgga cgtgaaccgg
     9001 agcgccgcgt cgttcacggt cgagggggac gcgatccggt tcggcctggc cgccgtccgc
     9061 aacgtgggga ccacggccgt cgactcgatg atcgccgcca ggacctccgg cggccccttc
     9121 accgggctcg ccgatttctg cgcgcgggtg gacacccggc aggtcaacca gcgggtggtc
     9181 gctagcctgg tcaaggccgg ggcgctggac gcgccggggg agccccgcgc caggatgctg
     9241 cagaccctcg acgacaccat ggagcaggcc cagcgggcgc agcgggtgcg ggcccagggc
     9301 cagaccggcc tcttcgacat gggcagcgag gcgccccccc tggccgacgc gtcgggagac
     9361 gagttcagcc ccgaagagct gctcaccatg gagaaggaga tgctcgggct ctacatctcc
     9421 gatcatccgc tccaccgctg gcagcccgtg ctggcgcagc gggtcaccgc gcagctggcg
     9481 cacctcccgg acctccccga ccgcaaggag gtcgtggtcg gcgggctggt gagcggcatg
     9541 aagcgaacgc tcacccgctc gggctccgtg atggcgtttc tgaccctgga ggacctcacc
     9601 ggaagcgtcg aggtggtcgt ctttccccgg gtctacgagc agcaggcgtc cgcgctcaag
     9661 cgggacgcgg tgctgctcct ccggggcaag gtggacatcc aggagcagag cgcgaagttc
     9721 ctgtgcgagg aggtcctgcc gctgccgtcg tctccggatc cggcggcccc cgacggcgac
     9781 cccgcgcgcg cggcctcccc cccgccgggg cgcccggctg acggcggcga ggactcggag
     9841 gatcccgccc gacccccgct ccgggtccgc gtgagcaccc gcgaggaggt cgcgcagctc
     9901 gagcggtacc tgctcgacca cccgggggac cgccaggtct gcgtgcacgt cgtcaccgac
     9961 cagggcgaag agcacatcgt cccggcgcgc gtccgcctcc acgacgccga cgggctgcat
    10021 caggaactgg agcagatctt cggcgagggc aacgtctggg aggagtgagg gcaatgatgc
    10081 gagcacggac gttcggcagg ggatcactct cggcactctg tgtctttctg gtgggcgcgt
    10141 tagtgacgcc cggtgtggcg ctcgccaaga cggcgcagga gatcgatgcg ggcgtcaatg
    10201 cggccctgga ccggttcacg agccaggtca agggatccaa ggagctcctc cagcgagcca
    10261 agggcgtcct cgtgttcgcg ggcgtcctgc aggcgggctt tgtgtatggg ggcgagtacg
    10321 gcgagggggc gctgcgcctc ggcggcaaga ccagaaccta ctacaatatc gcgcccgcct
    10381 cgttcgggtt acagctggga gcccagaaaa aggacatcat cctcgtgttc ttgcaggaca
    10441 aggcgctcaa ggactttcaa gccaagagcg gctggcagat cggggtcgac gcatcggtcg
    10501 tgctggtgga cgtcggggcc caaggctcga tagactcgac gaagctgaat cagccgatcg
    10561 tgggattcgt ggtgggccaa tcgggcctga tgtacaatct gtcgctgtca gggtcgaaga
    10621 tctcgaggct ggagaaatag gccccgttgg gagcaatcac acctcgggct cgaggacgag
    10681 gtcgaacgtc cccgtggtgg ccccggcctg cggcaggtag atcgtcacga gcgccttgta
    10741 tccggggacg gcaacgcgca ggtggatgtg gggctgaaag aagccgccgc cacccccggg
    10801 gaagttgctt tcaaaacggt accggccgtt gccatcggtg atgacggtgc cgcggtggac
    10861 gtcatcgtac tgcccatcgg gcgcccgaag ccagaactcg acccgggctc cctgcatggc
    10921 cgcacaatc
//
LOCUS       DASQGH010000540         7330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27808, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000540 DASQGH010000000
VERSION     DASQGH010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27808"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..519
                     /gene="ssb"
                     /locus_tag="VEW91_10630"
     CDS             112..519
                     /gene="ssb"
                     /locus_tag="VEW91_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYM92076.1"
                     /translation="MLNRIQLIGRLTRDPELRYLASGHPMAQFTVAVDRDFRNAAGER
                     DADFINCVAWRKLAEQVGQYCARGRLVAVEGRLQTRAYEAQDGSRRRVTEVIGDRVWF
                     LDSRKTEADPGADAPAEAHPEVIEDSETSPEET"
     gene            868..1737
                     /locus_tag="VEW91_10635"
     CDS             868..1737
                     /locus_tag="VEW91_10635"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HYM92077.1"
                     /translation="MIEMTLASAVALLRMTPRDRALVEVLNELRYLTVPQIQQVCYPS
                     ASVQTTSHRLTVLRRRRVLACLAHRTFDDRRAFWGLAPLGRAVAGSLAGSPPDTPRAC
                     ALAAVQMEHLIATNQVFCDLCAQGRAGRLGPFRWFGSHHACVDLGETRVVPDAVILAV
                     TSDGAAWSYCLELDQGTMGPAALAAKFRRYRALRQFAGLRRHEPAWEARASGWILFAC
                     PDARRAALGAQLAAECGLERFWAGTAADLPGNLAESIGLDLVPPVDSLPGLSGGIIPP
                     AVPATPYEGEEHQ"
     gene            1734..3362
                     /locus_tag="VEW91_10640"
     CDS             1734..3362
                     /locus_tag="VEW91_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HYM92078.1"
                     /translation="MRWSPLVWVAFLGVVAGWAMGASAPGPFLVLDIASAVLLIALLM
                     KGRRPRVHPGPLAGARWGDRTDAAPMRTPGPFLLGLWGSGVPLYLTEEQLSGHVLVVG
                     PSRTGKTAGAIAPNLLLRDPARESVVVLDVKTGPRSLWNVTAGRYGARAHLFCPFFEG
                     SIGYNPLARIDSIGAAQRKAWLLVQNTTPRDLSGDARVYAAATGDLATLLFLHVQQDR
                     GRGGHTVGSVYRLAMEGPTFVRETLRSSRIAEVCERQGIFSARERRVQEAAVTGLLER
                     LAPWADPLVVEATSHHHDLATIGREPTALYILMPETDAPRLQPLVAWLIADLLDELIE
                     QAERVGLRCPVRMYLDEFRRFGYLPGLSQSLPTLRERGISVLLGVQVLSQIEEVYGRV
                     EARTLLSNTETKLLFRAGDLETARMISDWLGCTTVPAVSVTTRGGRDRSTTVHPRVRP
                     LLPPEEVSRIPEGAMIALAGASRPLALWQARYYATPGFVVRHPPFQLRRREAPQLAVA
                     SAAPSIPVPAPRVAPPPAGGIPPRPPIGGDPTNA"
     gene            3450..4226
                     /locus_tag="VEW91_10645"
     CDS             3450..4226
                     /locus_tag="VEW91_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYM92079.1"
                     /translation="MWVDAHLHLDAPAFDADRDAVIGRAVAAGVRILVSAGTSIASSR
                     RTIALAEQYPEVVAAVGIHPGAAAEATPASLAALREMVRHPRAVAVGEIGLDYYREAT
                     PRHEQVQAFRAQVRLAREVGLPVVVHDRDADADIDGILQDEGVTRVILHCFSGTPERA
                     LRCAAAGWTLSLAGPLTFARPSALREVAKCVPLDRVLVETDAPYLAPVPFRGRRCEPA
                     FLPYTARALASLRELDGQALARQMAENARRLFPLIGADDA"
     gene            4219..4764
                     /locus_tag="VEW91_10650"
     CDS             4219..4764
                     /locus_tag="VEW91_10650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYM92080.1"
                     /translation="MPEQPAQARAVLWPMPLAIAVGCAALAARSSPGTGALLATVVAG
                     VLGGLLPVPGGEAPRASTIRWLGALGLGVAALVIGRLIRAPVAASPVTAFAVAANILA
                     AVSEELFFRRLMYAWLVRWGPALAIAATAAAFALVHVRAYGILVLPIDAAAGVLFGWQ
                     RWASGGWSAPAVTHALANLLP"
     gene            4839..5402
                     /locus_tag="VEW91_10655"
     CDS             4839..5402
                     /locus_tag="VEW91_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92081.1"
                     /translation="MIAALVYVGVVALYPVPIRLVYDGLAPLPPYRWVHPPGERAGDN
                     QPPLPGAGIITFGPLGSRPAEVATGDDQALVTFPEGVIPLRAGETSVRVAITPLDPAA
                     VAPPPGGQRFDGNAYRMEAIYATSAVPAALAGAVTVVLRYPVHGTLILRSMDPGWKAL
                     PTTRFDASQQVLANSDVLGIFVAAAAR"
     gene            5516..6649
                     /locus_tag="VEW91_10660"
     CDS             5516..6649
                     /locus_tag="VEW91_10660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYM92082.1"
                     /translation="MRGWGTGCRRAAAVLLGLVLCVSFTLPVAGADQTADEINVGAQA
                     AKEIESHYRVVTDPAMVERLTRVGDALTRVVDRQDLTYKFKVIDIPGVNALGVPGGWV
                     YVTRGMMRFVRTDHELAAVLAHELTHIAHRHYYIQQDRQSRMMPALILAAALSVLAHS
                     AAPLAGVAVATQGALANYQRDLERDADLTGVSYLTKTSYSPVAMLTLMEHLDQVDKLA
                     GRPDLGPLYQDHPLPDERVGYIQDDLRALGIPIIRRVPEGYLALWLDPSASVGGEPVT
                     VLVDGRPVIELGATVQGQTPIDRARALAANLNAFFNTDPAPYDVRAVGLQGQWSVIGG
                     QLRLFEVTPQDAAYARGSPQVVAEQLRGRLAEVLAAAPYNRKF"
     gene            6655..>7330
                     /locus_tag="VEW91_10665"
     CDS             6655..>7330
                     /locus_tag="VEW91_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HYM92083.1"
                     /translation="MDLHVAFLPEEAGALRGRVAVVIDVIRATTSLLAMLERGCAEVL
                     IAPTLEAARRYREAHVDVLLAGEQGGRAPEGFDFGNSPMEFAGAALAGRRVVFATTNG
                     TRAMRLAQDATVVFLGCLRNRSAAARAALAAAAEAGVGISVVCAGRQGRFSLDDAYTA
                     GAIVETLTDHNGGKIDLTDAGVAARQLYRSAGDPVALFRQTRAGRRVIEIGLGNDLEY
                     CAKIDQS"
BASE COUNT          989 a         2539 c         2629 g         1173 t
ORIGIN      
        1 gcggcggcag gtgcagacgg ggcggccctt cgtggagtcc cgggtcgtga tcgcgcaggg
       61 gtcaggtgcc aggctcgaga gtatcgttga tggacgtaga aggagggatc aatgctgaac
      121 cggattcagt tgattggacg actgacgcgc gatccagagc tccgatacct cgccagcggc
      181 cacccgatgg cgcagttcac ggtggcggtg gacagagatt tcaggaacgc cgccggcgag
      241 cgggatgcag acttcatcaa ctgtgtcgcc tggagaaagc tggccgagca agtcggtcag
      301 tattgtgccc gaggccgcct ggtcgccgtc gagggtcgcc tccagacccg tgcatatgag
      361 gcccaggatg gctcgcgacg gcgggtgacc gaggtgatcg gggatcgggt gtggttcctg
      421 gacagccgga agacggaggc tgatcctggt gccgacgccc ccgccgaggc ccaccccgag
      481 gtcatcgagg attccgagac ctcgcctgag gaaacgtaat cgcctggagt cagggtcgcg
      541 cgggcgagca tcccggtgga tgctgcgtta ggcgcgacgt accctgctgt gccccttttg
      601 tggcccagcc tggatttccc tcggatgatt caccccaccg ggtgatccgg aggacctaac
      661 acacctcggt ccgcggtcta tccggacggg ggggggcacg attccaagag ccccctggag
      721 gccgccgtgg tagcggcgtg atcccccggt cctgacccgg aaaacctgat ctcgaaaact
      781 gccgtgcccc cgccgcccgc ggacagatcg gtccgcgggc ttggtctgtt cgtccatgat
      841 cgcactgcca atccgaacgg aagggagatg atcgagatga cgctcgcgtc cgctgttgcg
      901 ctgttgcgga tgacccctcg cgaccgcgcc ctggtcgagg tgctcaacga actgcgctac
      961 ctgaccgtgc cgcagatcca gcaggtctgc tacccgtccg cgtccgtcca gaccacctca
     1021 caccggctga cggtcctccg ccggcgcagg gtgctggcct gccttgcgca ccggacgttc
     1081 gatgaccggc gtgcgttctg gggcctcgcc cctcttggtc gcgccgtcgc gggatcgctg
     1141 gcgggctctc cgcccgatac gccgcgcgcg tgcgccctgg ccgccgtcca gatggagcac
     1201 ctgatcgcta ccaaccaggt cttctgcgat ctgtgcgccc agggccgggc gggccgcctg
     1261 ggcccgttcc ggtggtttgg aagccatcac gcctgcgtgg acctcggcga gacccgggtg
     1321 gtgcccgacg ccgtgatcct ggccgtcacc tctgacggcg ccgcgtggtc atactgcctg
     1381 gagctcgacc agggcacgat ggggcccgcg gcgctggcgg ccaagttcag gcgctaccga
     1441 gccctccgcc agttcgcggg cctccggcgg cacgagccgg cctgggaagc ccgcgcctcg
     1501 ggctggattc tgttcgcctg cccggatgcg cgccgcgcgg cgctcggggc gcagttggcc
     1561 gccgagtgtg gcctggagcg attctgggcc ggcaccgcgg cggaccttcc gggcaacctg
     1621 gccgaatcga tcggcctcga cctggtcccg ccggtggatt ccctgcctgg gctatccggg
     1681 gggatcatcc ccccggccgt tcccgccacg ccgtacgagg gggaggaaca ccaatgaggt
     1741 ggtcgccgtt ggtctgggtc gcattcctcg gagtggtcgc cgggtgggcg atgggggcat
     1801 ctgcccccgg cccgttcctg gtcctggaca ttgcctcggc cgtgctcctc atcgccctcc
     1861 tgatgaaggg gcgccgccca cgggtacacc ccggaccgct cgccggggcg cggtggggag
     1921 accggacgga tgccgccccc atgaggaccc cgggcccgtt cctgctcggc ctctgggggt
     1981 ccggcgtccc tctctacctc accgaagagc aactctccgg ccacgtgctg gtggtcggac
     2041 cttcgcgaac ggggaagacg gcgggggcga tcgcccccaa cctcctcctt cgtgaccccg
     2101 ccagggagag cgtcgtggtc ctcgacgtga agaccgggcc ccggtccctc tggaacgtca
     2161 ccgcgggccg gtatggcgcc cgggcccacc tgttctgccc gttcttcgaa ggctcgatcg
     2221 gctacaaccc cctggcccgc atcgactcga tcggcgcggc ccagcggaag gcgtggctgc
     2281 tcgtgcagaa caccacgccc cgtgatctct cgggcgacgc ccgagtgtac gcggcggcca
     2341 ccggggatct cgcgacgctg ttgttcctgc acgtccagca ggatcggggc cgtggcggcc
     2401 acaccgtcgg atcggtgtac cggctcgcga tggaggggcc gacgttcgtc cgggagacgc
     2461 tccggagcag ccgaatcgcc gaggtgtgcg agcgccaggg aatcttctcg gcgcgcgagc
     2521 gccgcgtgca ggaggcggcg gtgaccggcc tgctggagcg gctggccccg tgggccgatc
     2581 cgctggtggt ggaagcgaca tcccaccacc atgacctcgc caccatcggg cgcgagccca
     2641 cggcgctcta catcctcatg ccggagaccg atgcgcctcg tctgcaacct ctggtcgcct
     2701 ggctgatcgc cgacctgctg gacgagctca tcgagcaggc ggaacgggtg gggctgcgct
     2761 gccccgttcg gatgtacctc gatgagttca gacggttcgg atacctcccg ggcctgagcc
     2821 aatcgctgcc gaccctgcgg gagcgcggca tcagcgtcct cctgggggtc caggtgttga
     2881 gccagatcga ggaagtctac gggcgcgtgg aggcacgcac cctgctcagc aataccgaga
     2941 caaagctcct gttccgcgcg ggagacctgg agaccgcccg gatgatcagc gactggctcg
     3001 ggtgcaccac cgtgccggcg gtgtcggtga cgacccgggg gggacgggac cgcagcacca
     3061 ccgtgcatcc ccgcgttcgc ccgctcttgc ccccggagga ggtcagccgg atcccggagg
     3121 gcgcgatgat cgccctggcc ggcgcgtccc ggccgctggc gctgtggcag gcacggtact
     3181 acgcgacccc cgggtttgtg gtccggcacc cgccgtttca gttgcgccgg cgcgaggcgc
     3241 cgcagcttgc ggtcgcatcg gcggcaccat ccatcccagt ccctgccccc cgggttgccc
     3301 ctccgccagc cggagggatt ccgccgcggc cccctatcgg aggagacccc accaatgcgt
     3361 agaacccgtc cacggcccct gccccggagc cctgcgcgca gggcatccgg gcagggttcc
     3421 ggggagggcg ccgcgcgtcg aggctcagca tgtgggtcga tgcacacctg cacctggatg
     3481 cgccggcgtt cgatgcggac cgcgacgcgg tgatcggtcg cgccgtcgcg gccggcgtga
     3541 ggatcctggt gtcggcgggc acgtcgattg ccagcagccg tcgcacgatc gccctggccg
     3601 agcagtatcc cgaggtcgtc gcggccgtcg gcatccaccc cggagcggcc gccgaggcga
     3661 ccccggcgtc gctggcagcg cttcgagaga tggttcgcca cccccgagcc gtcgccgtcg
     3721 gtgaaatcgg gttggactac tatcgagagg ccaccccgcg ccacgaacag gtccaggcgt
     3781 tccgggcgca ggtgcggctg gcccgcgagg tcggcctgcc cgtggtggtg cacgatcgtg
     3841 atgcggacgc cgacatcgat gggatcctgc aggatgaggg agtgacccgg gtgatcctgc
     3901 actgcttttc cgggactccg gagcgggcgc tgcggtgcgc ggcagccggc tggacgctct
     3961 cgctggcggg tccgctgacc tttgcgcgcc cgagcgccct gcgggaggtc gcgaagtgcg
     4021 tcccattgga ccgggtcctg gtggaaaccg atgcgccgta cctggccccc gtcccgttcc
     4081 gcggccgccg atgcgagccg gcgttcttgc cctacacggc gcgcgcgctg gcatccctcc
     4141 gcgaactgga cggtcaggcc ctggcccggc agatggcgga gaacgccaga cggctcttcc
     4201 ctttgatcgg cgcagacgat gcctgagcag ccggcgcagg cccgcgcggt gctctggccg
     4261 atgccgctgg ccatcgcggt tgggtgtgct gccctcgctg cccggagttc ccccggcacg
     4321 ggtgcgctcc tggccaccgt cgtcgccgga gtgctcgggg gcctgcttcc ggtgccgggc
     4381 ggagaggcgc cgcgggcctc taccattcgc tggctcggcg ccctggggct gggggtcgcg
     4441 gcactggtca tcggtcgcct gatccgcgca ccggtcgcgg cgagcccggt gaccgccttc
     4501 gccgtcgcgg cgaacatcct cgccgccgtc tccgaagagc tgttctttcg gcggctgatg
     4561 tacgcttggc tcgtcagatg ggggccggcg ctggccattg ccgcaaccgc cgctgcgttc
     4621 gcgctcgtcc atgtccgcgc ctatggcatc ctcgtgctcc cgatcgacgc cgccgcgggc
     4681 gtcctgttcg gctggcagcg ctgggcctcg ggaggctggt ccgctccggc cgtcacgcac
     4741 gcgctggcga atcttcttcc gtgaccatgc gcggaccaga acgtcggcct gcccgcagcg
     4801 cggagcgctc cggcggcacc cgtcggatcg cgtgggggat gatcgcggcc ctcgtctatg
     4861 tcggcgtggt cgcgctctat ccggtcccga tccgcctcgt gtacgacgga ctggctccgc
     4921 ttcccccata ccgatgggtc cacccgcccg gcgaacgcgc cggggacaac caaccccccc
     4981 tgccgggcgc cggcatcatc accttcggcc cgttgggctc acggcccgcc gaggtggcca
     5041 cgggggacga ccaggcgctg gtcacattcc ctgagggcgt gatccccctg cgggcgggcg
     5101 agacctccgt gagagtcgcg atcaccccgt tggacccggc cgctgtggct cccccaccgg
     5161 gcggtcagcg cttcgatgga aacgcctatc gaatggaggc gatctatgcg acctcggcgg
     5221 tgccggcggc cctcgccggc gccgtgacgg tggtcctccg gtatccggtc catggcacgc
     5281 tgatcctccg gtcgatggat cctggatgga aggcgctgcc caccacccgg ttcgatgcga
     5341 gccagcaggt cctcgccaac tccgacgtgc ttgggatttt cgtcgccgcc gcggcccgct
     5401 agcgccgggg cgcaccggcc ggcgttctcg gtgtaacgaa ccccgggggc ccaatcgtat
     5461 atcgtataat gaaggcgtgg cgagtcggtg ggggggaggc ttgaggacgg cgagaatgcg
     5521 gggttggggg acggggtgcc gccgggccgc cgccgtgctc ctcggtctgg tgctctgcgt
     5581 ctcgttcacg ctccctgtcg caggggccga ccagacagcc gacgagatca acgtcggggc
     5641 tcaggccgca aaagaaatcg agtcgcatta ccgcgtggtc accgatccgg cgatggtcga
     5701 gcgcctcacg cgggtcgggg atgcgttgac ccgcgtcgtg gaccgccagg acctgacgta
     5761 caagttcaag gtcatcgaca tcccgggggt gaacgccctc ggcgtccccg gcggctgggt
     5821 gtacgtgacc agggggatga tgcgatttgt ccggacggac cacgagctcg cggccgttct
     5881 tgcgcacgaa ctcacccata tcgcccaccg ccactactac atccagcagg accggcagag
     5941 ccggatgatg ccggcgctca ttctggccgc cgccctatcg gtgctcgccc actcggcggc
     6001 ccccctggcc ggtgtggcgg tcgccaccca gggcgcgctg gccaactacc agcgcgatct
     6061 cgagcgagac gcggacctga cgggcgtctc gtacctcacg aagacgagct actcgccggt
     6121 ggccatgctg accttgatgg agcacctgga ccaggtggac aagctggccg gccggccgga
     6181 cctggggccc ctctaccagg atcaccccct cccggacgag cgggtcggct acatccaaga
     6241 cgatctgaga gcgctgggca tcccgatcat ccggcgggtt ccggaggggt acctggcgct
     6301 ctggcttgac ccgtccgctt cggtgggggg cgagccggtg acggtcctcg tggacgggcg
     6361 accggtcatt gagctcggcg caaccgtgca ggggcagacc ccgatcgatc gggcccgggc
     6421 gctggcggcg aacctgaacg cctttttcaa caccgacccg gcaccctatg atgtgcgtgc
     6481 ggtgggcctc cagggtcagt ggtcggtgat cggcggccag ctgcggctct tcgaggtcac
     6541 gccccaggat gccgcgtatg cgaggggatc gccacaggtg gtcgcggagc agctgcgggg
     6601 tcggctggcc gaggtgctgg cggcggcgcc gtataatagg aagttttagc tcaggtggac
     6661 ctccatgtcg cgttcctccc cgaggaggcc ggagcactcc gcgggcgcgt cgccgtggtg
     6721 atcgatgtca tccgcgccac cacgtccctg ctggcgatgc tggagcgggg ctgtgcggag
     6781 gtgctgatcg ccccgaccct ggaggccgcc cgccggtatc gcgaagccca cgtggatgtc
     6841 ctgctcgcgg gcgagcaggg cggccgggct cccgaggggt tcgactttgg caacagcccg
     6901 atggagttcg ccggggccgc gctcgccggc cggcgggtgg tgtttgcgac gacgaacggc
     6961 acccgggcga tgcggctcgc ccaggacgcc accgtggtct tcctgggatg cctccggaac
     7021 cggtcggcag cggcccgggc cgcgctggcc gcggcggcgg aggcgggggt agggatctcg
     7081 gtggtgtgcg cggggcggca ggggcgcttc agtctggatg acgcctacac ggccggcgcc
     7141 attgtggaga cgctgacgga tcacaacgga gggaagatcg acctgacgga tgccggggtg
     7201 gctgcccgcc agctctatcg gagtgcgggg gatccggtgg cgctgttccg ccagacgcgc
     7261 gcggggcgac gcgtgatcga gatcggcctc gggaacgacc tcgagtactg cgccaagata
     7321 gaccagtcga
//
LOCUS       DASQGH010000541         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10741, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000541 DASQGH010000000
VERSION     DASQGH010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10741"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..956
                     /gene="folP"
                     /locus_tag="VEW91_10670"
     CDS             105..956
                     /gene="folP"
                     /locus_tag="VEW91_10670"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYM92084.1"
                     /translation="MTVPPIRCGGFRIEFGRRTLIVGIINMAPDSFAGDGLAADADAA
                     EARGHEMRAAGADLLDIGGRSTRPGAPPVEVEEELRRTIPAIRRLAGIGVPVSVDTFS
                     ARVAAAGLDAGAVMVNDVSGLRADPGMAPLVSRTGVPVVVMDWDDRHGARDVVQETAA
                     FLRERIAAAAAAGIAETQIVVDPGYGFGLTLEQDFDILRRLRELAALGRPILIGTSRK
                     GSIGKVLHLPVHDRLEGTAATVVVAIMNGADMVRAHDVRAISRVARMTDAIVRGGGSM
                     NDSAETT"
     gene            953..1315
                     /gene="folB"
                     /locus_tag="VEW91_10675"
     CDS             953..1315
                     /gene="folB"
                     /locus_tag="VEW91_10675"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HYM92085.1"
                     /translation="MTDRILLSEMAFYAHHGLTPEEQARGQVFIVDVTVEANLHRAGH
                     TDEIADSLDYRDLYARVKQVLTEERYRLLEAVAEAVAHRVLEADRVEAVTVRVRKPHV
                     QLGGPLAYAAVEVTRRRL"
     gene            1312..2490
                     /gene="folK"
                     /locus_tag="VEW91_10680"
     CDS             1312..2490
                     /gene="folK"
                     /locus_tag="VEW91_10680"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYM92086.1"
                     /translation="MSGAPPLRRVEALHRAIRLEQVSVVWMIVEGGIAVVSGIAAGSL
                     ALAAFGWDSLIETITALAVLWRAVVESRVWQAAAGSAASVAAAARSEAAERTAARVVA
                     AGLVALALYVLVGAVQDLRDHRVARPGVWGLAVSAVAVAGMPVLYAAKRRVARVLDSE
                     ALAEDAAGNLTCGLMAGILLAGLLVQRGGMWWADSVAALLLGLLVAREGWEAWRRAGE
                     GSARAVRVFLGLGSNQGDRAEMLARARALLAAPDLRLVAASRVYETLPWGDVDQPRFL
                     NQVIEAQTTLSPRALLGRCREVEARLGRVRGRRWGPRTIDVDILVYGNVEMAEPDLVI
                     PHPELRRRAFVLISLVELDPGLRLPGGETVEGLLEALPDRGGVGGYDPEVHPAAGGDR
                     "
     gene            2487..3296
                     /locus_tag="VEW91_10685"
     CDS             2487..3296
                     /locus_tag="VEW91_10685"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYM92087.1"
                     /translation="MRTAGDEAGEAGRERALADLGTRLVGRYVRWHDSLPSTNDLAVR
                     LTEIPVPEGTVIGAEEQTAGRGRWGRPWASPRGGIWLSVILRPGLPADRMPVVGLAAA
                     VAAAQAIRETTGLPARVKWPNDVLVHGQKVVGILAEATPGAEWVVVGVGINANIAQEA
                     LPHTPGYPATSLQVLLGHPVDRGSLIRALLRGLDRGYELLGSAGVRAVLARWREMADT
                     LGRKVQVEMPNTLVQGVAHDIDETGALLVRCDDGAVRKVIAGDLRIREAGR"
     gene            3293..>3710
                     /locus_tag="VEW91_10690"
     CDS             3293..>3710
                     /locus_tag="VEW91_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HYM92088.1"
                     /translation="MITDVPGITVGHATDLGHLTGCTAVLCAEGAVAGGVVMGGAPGT
                     RETDILRPENLVEHVHAVLLCGGSAFGLAAVDGAVRYLEERGVGYPTPHAKVPIVPAA
                     VIYDLGIGSPHIRPDAAMGEAACRAARAGPVEEGCVG"
BASE COUNT          460 a         1204 c         1488 g          558 t
ORIGIN      
        1 gtggccgccc tgcgccttcg gcgcctccgc ccggggcagc tccttgatcc ggcggggctc
       61 gcgcgcgacg tcgacggtgg gggtgcgggg ccgggagacc cccgatgacc gtccccccga
      121 ttcggtgtgg gggcttccgg atcgagttcg ggcggcgcac cctgatcgtg gggatcatca
      181 acatggcgcc cgattcgttt gccggggacg ggctggccgc cgatgccgac gccgccgagg
      241 cccgtgggca cgagatgcgg gctgcggggg cggatctcct cgacatcggg ggccggtcga
      301 cccgtccggg cgctcccccg gtggaggtgg aggaggagct ccgacggacg atcccggcga
      361 tccggcggct cgccgggatc ggcgtgccgg tcagcgtgga tacgttttct gcgcgcgtgg
      421 cggcggccgg ccttgacgcg ggggcggtga tggtgaacga cgtctcgggc ctgcgggccg
      481 atccgggcat ggccccgctc gtgtcccgga ccggggtgcc cgtggtcgtg atggactggg
      541 acgaccggca tggcgcccgg gacgtggtgc aggagaccgc ggcgtttctc agagaacgga
      601 ttgccgccgc ggcggcggcg gggatcgccg agacccagat cgtggtcgac cccgggtacg
      661 gcttcggact cacgctggag caagactttg atattctccg gcgcctgcgc gaactggcgg
      721 cgctcggccg cccgatcctc atcgggacgt cccgaaaagg gagcatcggc aaggtcttgc
      781 accttccggt gcacgaccgt ctggagggca cggctgcgac ggtggtggtg gcgatcatga
      841 acggcgccga catggtgcgg gcccacgacg ttcgggcgat ctcccgggtg gcccggatga
      901 ccgatgcgat cgtccggggc ggcggatcca tgaacgactc ggccgagacg acgtgaccga
      961 ccggatcctg ctttccgaga tggcgttcta cgcccatcat gggctgaccc cggaggaaca
     1021 ggcccggggg caggtgttca tcgtcgacgt caccgtcgag gccaatctcc accgggccgg
     1081 gcacaccgac gagatcgccg actccctcga ctaccgggat ctctatgcgc gggtcaagca
     1141 ggtgttgacg gaagaacgat accggctcct ggaggcggtg gccgaggcgg tggcgcaccg
     1201 cgtgctggag gccgaccggg ttgaggcggt caccgtgcgg gtcaggaagc cccatgtgca
     1261 gctgggcggt cccctcgcgt atgccgccgt cgaggtcacc cggcggcggc tgtgagtggc
     1321 gctccgcccc tccggcgcgt tgaggcgctc catcgggcca tccgcctcga acaggtctcg
     1381 gtcgtctgga tgatcgtgga gggcggcatc gccgtggtct ctgggatcgc cgccggcagc
     1441 ctcgccctgg cggcctttgg gtgggacagc ctgatcgaga cgatcacggc cctcgcggtg
     1501 ctgtggcgtg cggtggtcga gtcgcgcgtg tggcaggctg cggccgggtc ggcggcctcc
     1561 gttgccgccg cggcccgcag cgaagcggcc gagcgcaccg cggcccgcgt ggtggccgcc
     1621 gggctcgtgg cgctggcgct ctacgtcctc gtcggggcgg tgcaggacct tcgcgatcac
     1681 cgcgtggccc gccccggggt ctggggtctg gcggtttccg cggtggcggt tgcgggcatg
     1741 ccggtgctgt acgccgcaaa gcggcgggtg gcccgcgtcc tggacagcga ggcgctggcg
     1801 gaggatgccg ccggcaactt gacctgcggg ctcatggccg gcatcttgct cgccggcctg
     1861 ctcgtgcagc gtggggggat gtggtgggcc gattcggtgg cggcgctgct cctcggcctg
     1921 ctcgtggccc gggaaggctg ggaggcctgg aggcgggcgg gcgaaggatc ggcgcgggcg
     1981 gtccgggtgt ttctgggcct cgggtcgaac cagggagacc gcgcggagat gctggcccgc
     2041 gcgcgggcgc tgctcgctgc gccggatctt cgcctcgtcg cggcctcgcg ggtgtatgaa
     2101 acgctcccct ggggcgacgt cgatcaaccc cggttcctca accaggtgat cgaggcgcag
     2161 accaccttgt ccccccgggc gttgctcggg cggtgccgcg aggtcgaagc gcgattgggc
     2221 cgcgtccggg ggcggcgatg ggggccccga acgatcgacg tggacatcct ggtctacggg
     2281 aatgtcgaga tggccgaacc cgacctcgtg atcccgcacc cggagctgcg ccgccgggcg
     2341 ttcgtgctga tctcgcttgt cgagctcgac ccgggcctcc ggctgccggg cggagagacg
     2401 gtcgaagggc tcctggaagc gctccccgac cgcgggggcg tcggcgggta cgacccggag
     2461 gtccatccgg cggcgggagg ggaccgatga ggacggcagg ggacgaggcg ggtgaggccg
     2521 ggcgcgagcg ggcgctggcc gacctaggca cacgcctggt tgggcgctat gtgcggtggc
     2581 acgactctct gccctccaca aacgacctcg cggtgcgctt gacggagatc ccggtcccgg
     2641 aggggacggt catcggggcg gaggagcaga cggcgggccg gggacgatgg gggcgtccgt
     2701 gggcctcccc gcggggaggg atctggctct cggtgatcct caggccaggg cttcccgcgg
     2761 atcgcatgcc cgtggtcggg ctcgccgctg ccgtggccgc cgcccaggcg attcgcgaaa
     2821 ccaccgggct ccccgcccgg gtcaaatggc ccaatgacgt cctggtgcac gggcagaaag
     2881 tcgtcgggat cctcgccgaa gcgaccccgg gcgccgagtg ggtggtggtg ggggtcggca
     2941 tcaacgccaa tatcgcgcag gaggccctgc cgcacacgcc ggggtatccc gccacctccc
     3001 tgcaggtcct cctcggtcac ccggtggatc ggggaagcct gatccgcgcg ctgctgcgcg
     3061 ggctggatcg agggtacgaa ctgctcgggt ccgcgggggt gcgcgcggtg ctcgcgcgct
     3121 ggcgggagat ggcggatacc ctgggccgca aggtccaggt cgagatgccg aacacgctgg
     3181 tgcagggggt cgcgcacgac atcgatgaga ccggcgcgtt gctcgtccgg tgtgatgacg
     3241 gagccgtccg gaaggtgatc gccggcgatc tgcggattcg cgaggcaggg cgatgatcac
     3301 cgatgtgccc gggatcaccg tcgggcatgc gaccgacctg ggtcacctga cgggttgcac
     3361 ggcggtgctc tgcgcggagg gcgccgtcgc cgggggcgtg gtcatgggcg gcgcccccgg
     3421 aacccgcgag accgacatcc tcaggcccga gaacctcgtc gagcacgtcc acgccgttct
     3481 gctgtgcggc ggcagtgcgt ttgggctggc ggcggtggac ggggcggtcc ggtatctcga
     3541 ggagcgcggg gtgggctatc ccaccccgca tgcgaaggtg ccgatcgttc ccgcagccgt
     3601 gatctacgac ctcgggatcg gcagcccgca catccggccg gacgcggcga tgggcgaggc
     3661 ggcgtgccgg gcggcgcggg cggggccggt cgaggaaggg tgcgtgggcg
//
LOCUS       DASQGH010000542         1749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47786, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000542 DASQGH010000000
VERSION     DASQGH010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47786"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VEW91_10695"
     CDS             <1..185
                     /locus_tag="VEW91_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM92089.1"
                     /translation="PSKMTRWLVEHRGEAILGTVPMGRLGGDDDLKGAAVFLASRASD
                     FVTGQVLVVDGGATAW"
     gene            209..1090
                     /locus_tag="VEW91_10700"
     CDS             209..1090
                     /locus_tag="VEW91_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HYM92090.1"
                     /translation="MEPNAVRRLCVVGAGTMGHQIAMLGALAGCDVTVVDVRPEALEK
                     AEASNRAHMARWVERGRLTGPQAADALGRLRWTTRLEEGAADADFAIEAVIERLEDKQ
                     AVFKDLDRLAPSHAILATNSSTIRSSLLASVTARGDRVCNMHFFNPPLVMELVEVVVH
                     PETSEATAAAAMTLARRMGRTPVLLRKEISGFVVNRILAAIMDEAVRLYEGGIASFED
                     IDIAVKKGLRHPMGPFELMDFNGIDVAYNVRMQRYRESGDALQKPARSIVERYEQGAW
                     GRKTGKGWYTYPPPGPS"
     gene            1117..>1749
                     /locus_tag="VEW91_10705"
     CDS             1117..>1749
                     /locus_tag="VEW91_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM92091.1"
                     /translation="MVTEQRPAGGMWLGEPSAAPEVFTPERLSDEHRMIARTVRTFLQ
                     QEVAPLDARLEQKDLPLMREVIRKLAALGVFASDVPAEYGGLGLDRMTGLLLAETISR
                     GSISSSVGAHLTIGMLPIVFFGTDAQRRHYLPRMVAGDLVGAYALTEPTAGSDALALR
                     ATAVLDADGAYRLSGAKQFITNGGIADVYVLYAKIDGSKLTCFIVDRNTPG"
BASE COUNT          276 a          557 c          658 g          258 t
ORIGIN      
        1 ttccgagcaa gatgacccgg tggctggtcg agcaccgggg agaggcgatc ctcgggaccg
       61 tccccatggg gcggctgggg ggtgatgacg acctgaaggg ggcggcggtg tttctggcca
      121 gccgggccag cgacttcgtg acggggcagg tcctggtggt ggatggggga gccacggctt
      181 ggtgaggtcc cccgagcggg gagggtgtat ggaaccgaac gcggtgcggc gcctgtgcgt
      241 ggtcggggcc gggacgatgg gccatcaaat cgcgatgctg ggcgcgctgg ccggctgcga
      301 cgtgaccgtg gtggatgtgc ggcccgaggc gctggagaag gccgaggcca gcaaccgcgc
      361 ccacatggcg cggtgggtgg agcggggacg gctcaccggc ccacaagcgg cggatgcgct
      421 tggccgcctc cggtggacca cccggctgga ggagggggcc gcggacgccg acttcgccat
      481 cgaggcggtg atcgagcggc ttgaggacaa gcaggcggtg ttcaaggacc ttgaccggct
      541 cgcgccatcg cacgcgatcc tcgcgaccaa ctcgagtacg atccggagct cgctcctcgc
      601 gtcggtgacg gcccggggcg accgggtctg caacatgcac ttcttcaatc cccccctggt
      661 gatggagctc gttgaggtgg tggtccatcc cgagacgagc gaggcgaccg cggcggcggc
      721 gatgaccctg gcccggcgga tggggcggac gccggtgctg ctccgaaagg agatctccgg
      781 gttcgtcgtc aatcgtatcc tggccgcgat catggacgag gcggtccgcc tctatgaggg
      841 cgggatcgcc tcgttcgagg acatcgacat cgcggtcaaa aagggtctcc ggcatccgat
      901 gggtccgttc gagctcatgg acttcaacgg gatcgacgtg gcctacaacg tccggatgca
      961 gcgctaccgt gagagcggag acgcgctcca aaagccggcc cgcagcatcg tggagcggta
     1021 cgaacagggc gcgtgggggc gcaagaccgg gaaggggtgg tacacgtacc cgccccccgg
     1081 cccgtcgtga ggcgagggcg cgatggtgag gggcgcatgg taaccgagca gcggcctgcg
     1141 ggaggcatgt ggctgggtga gccatccgca gcgcctgagg tgttcacccc ggagcggctc
     1201 tccgacgagc accgcatgat cgcgcggacg gtccggacct tcctccagca ggaggtggcg
     1261 cccctcgacg cccggcttga gcagaaggac ctgccgctca tgcgcgaggt catcagaaag
     1321 ctggccgccc tcggggtctt cgcatcggac gtgccggccg agtacggagg gctggggctc
     1381 gatcggatga ccggcctgct cctcgccgag acgatctcgc gcggctccat ctcgtcgagc
     1441 gtgggagcgc atctcacgat cggcatgctc cccatcgtct tcttcgggac ggacgcccag
     1501 cggaggcact acctgccgcg gatggtcgcg ggggacctgg tcggcgcgta cgccctgacc
     1561 gaacccaccg cgggctcgga tgcgctcgcg ctccgcgcca ccgcggtcct cgacgcggac
     1621 ggcgcctacc ggctgtcggg cgccaagcag ttcatcacca acggcggaat cgccgacgtc
     1681 tacgtgctct acgccaagat cgacgggagc aaactcacct gcttcatcgt cgaccggaac
     1741 accccgggg
//
LOCUS       DASQGH010000543         4501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55947, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000543 DASQGH010000000
VERSION     DASQGH010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55947"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(947..1468)
                     /locus_tag="VEW91_10710"
     CDS             complement(947..1468)
                     /locus_tag="VEW91_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HYM92092.1"
                     /translation="MRARVARPSDAPAIARIYNQGIEDRVATFETRRRSAGDVRAWFN
                     GVHPLVVVEDGGAVIAFAATSPYRPRECYAGVAEFSVYVAREARGRGAGRVAMEALVR
                     EAERSGFWKLVSRVFKENTPSLRLLASVGFREVGTYEKHARLDGVWRDVIIVERLLGT
                     LRPRARRSERRTR"
     gene            complement(1526..2293)
                     /locus_tag="VEW91_10715"
     CDS             complement(1526..2293)
                     /locus_tag="VEW91_10715"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYM92093.1"
                     /translation="MRLRDKVVLITGGGSGMGRLASAAFAREGARVVSVDVAAEAGSA
                     AVAEITRAGGQALFIEADVGREDQVRNAVRQAVARFGRLDVLYNNAGIFPADDGSVVN
                     LEETVWDRVMTVNLKGIYLCCKHGIPELIRAGGGSIINVASFVALVGCSVPQDAYTAS
                     KGGVIALTKSLAVQFGPQRIRTNAIAPGPVETPLLTSWLLANPEAKKRRLDRIPMGRF
                     GRPEDIVQMALYLASDESTWVNGSVLVVDGGITSNYF"
     gene            complement(2305..3687)
                     /locus_tag="VEW91_10720"
     CDS             complement(2305..3687)
                     /locus_tag="VEW91_10720"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYM92094.1"
                     /translation="MTEPAAGRLTLEALQEQVGAGEIDTVIAAMVDMQGRLVGKRVTG
                     RFFIDAVARDGAHACSYLLACDIEMEPLPGYRLASWATGYQDVWLRPDLGTLRRLPWL
                     EKTALVLCDVCDEAGQPVPESPRQILRRQVEQAQERGVLPMVASELEFYLFRETYEGA
                     RRKHYHDLEPYGQYIEDYHILQGTKEEWLVRQIRNGLEAAGVPCEFSKGEWGPGQHEI
                     NLRYTTPVEMADRHTIYKHAAKEIAALNGVALTFMAKWRTDLAGSSFHLHSSLWDRDG
                     KRSLFWEEGRTPLGMSSVFRHYLGGQLAGARQLAYLFAPYINSYKRYLAGTFAPVLAV
                     WGHDNRTCGFRVVGEGQSLRVENRIPGADANPYLAFSATIAAGLWGVERKVEPPALFE
                     GDAYKAEGLPRVPGSLREAIGELAGSELARWAFGDRVVEHYLHTGRLEQESYDRAVTC
                     WELGRNFERI"
     gene            3807..>4501
                     /locus_tag="VEW91_10725"
     CDS             3807..>4501
                     /locus_tag="VEW91_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HYM92095.1"
                     /translation="MAAMGVINGERLWGSLMSMAELGRTPGGGVRRLALSDEDRRARD
                     LLVRWLQEAGCTVRVDDLGNIYGRRDGEDPDAAPVVCGSHLDTVPTGGRFDGALGVLA
                     ALEAVRTMHDAGLRTRRAVEVVCWTNEEGVRFAPAMLASGVVCGRFTREEAYAAQDQD
                     GRTFEAELRRIGYLGGSEHRMRSPAAYIELHVEQGPILENEGAQVGVVEGVEGIAWGW
                     VDVTGRAAHAGPTP"
BASE COUNT          674 a         1604 c         1530 g          693 t
ORIGIN      
        1 atcccatccg gacgacagtt tcgcgattca ccagttcttc atcccgcgtt caccacttct
       61 tcatgacttc ttcgcaactg cctgctagcc tgaaggacaa ccgacgccac ccgggaggtg
      121 tagagagatg cgctatacca agagagcctg ggcagtcttc gctgcgtgtg ccgtgctgac
      181 tcccgtcctc cccggtcccg cgttgctggc tcagacgcct cagccgggga tcgctccggg
      241 accggtcagg cacgaaattg tcgtgaccgg tcggggctcc gtcagcgcca cgcctgatcg
      301 tatcaccatc gcgctggtca ccggggccgt ccggcccacg ttgcaggaag cccagcagca
      361 gagggacgcg gcggtcgcga aaattgtcca tgatgtgggc gcgctgggag tttctcaaca
      421 gagcatcgag acgaccacgt tcgtccgccc gcggcgcggg gagagattct cccgcgagcg
      481 caggtctcct ggctcccgga ggaccgggta cgccgcgatt gggcgaatcc tcgtgactgt
      541 cgatagcccg gccctcgcta cccgggtggt gaatgctgcc gccgcagccg gatccacccg
      601 agtcgcccgg gtcagggcgg gctggcgtga tccatcctcg taccatgccc aagcgctaaa
      661 attggccgtt gagcgtgccg aggcggacgc gaagaccatc gccaatgcag ccggggtacc
      721 gacacctcat ctcgcccaga tccaggaggt ccagccatcc gtgcaaaccc gtagaccgag
      781 cgggaggcgt ggcgcgtgga cctcccgcgc ggatgcccag ctgcggggga ccgctcggcc
      841 aatgcgcacc aggcgttccc cggtgccggt ggttccgcaa tcgcttccca tcaccgcgac
      901 agtgcgtgcg gtatatacgt tctgaagccg acacgccccc gtcggctcat cgggtccggc
      961 gctcgctgcg ccgggcccgg ggccggaggg tcccgagcag ccgctcgaca atgataacgt
     1021 cccgccagac gccgtcgagc cgcgcgtgct tttcataggt gcccacctcg cggaacccga
     1081 cggacgccag gaggcgaagg ctgggggtgt tctctttgaa cacccgcgaa acgagcttcc
     1141 agaaccccga tcgttctgcc tcgcgcacca gggcctccat cgccactcgc ccggccccgc
     1201 gcccgcgggc ttcgcgcgcc acgtacaccg agaactcggc cacgcccgcg tagcactccc
     1261 gcgggcggta gggtgacgtg gccgcgaacg cgatcaccgc accgccgtct tcgacgacca
     1321 cgagcgggtg gacgccgttg aaccaggcgc gcacgtcgcc ggcggaccgc cgccgcgtct
     1381 caaaggtggc gacgcggtcc tcgatccctt ggttgtagat tcgggcgatc gcgggcgcat
     1441 cgctcggcct ggccacccgg gcccgcaccg tcttcgcgtc acggagatcg cgggaagagc
     1501 gacggcgagg gggcacagcc ggcgttcaga agtagttgga cgtgatcccg ccgtccacga
     1561 cgagcacgct gccgttcacc cacgtggact cgtcggaggc gagatacagg gccatctgca
     1621 cgatgtcctc ggggcgcccg aagcggccca tcggaatccg gtccaggcgg cgcttcttgg
     1681 cctccgggtt cgcgagcagc cacgaggtca acagcggggt ctccacgggg cccggcgcga
     1741 tcgcgttggt gcggatgcgc tgggggccga actgcacggc cagcgacttg gtcagcgcga
     1801 tcaccccgcc cttgctcgcg gtgtaggcgt cctgcggcac cgagcaaccg accagcgcga
     1861 cgaacgaggc gacgttgatg atcgacccgc ccccggcccg gatcagctcg ggaatgccgt
     1921 gcttgcagca gagatagatc cccttcaggt tgacggtcat gacccggtcc cagaccgtct
     1981 cctccaggtt caccaccgac ccgtcgtccg ccgggaagat ccccgcgttg ttgtagagca
     2041 cgtcgaggcg gccgaaccgg gcgaccgcct gccggacggc gttccgaacc tgatcctcgc
     2101 gccccacgtc ggcctcgatg aagagcgcct gcccgccggc acgggtgatc tcggccaccg
     2161 cggccgaacc ggcttcggcc gccacatcca ccgagaccac ccgggcgcct tcgcgcgcaa
     2221 aggcggcgga cgccaggcgg cccattccgc tgccgccccc cgtgatcagg acgaccttat
     2281 cgcgcagtcg catcggtggc tccactagat gcgctcaaag ttccggccca gttcccagca
     2341 ggtgaccgcg cggtcatagc tttcctgctc gagccgcccg gtatggaggt agtgttcgac
     2401 cacgcggtcg ccgaacgccc agcgggcgag ctcgctgccc gccagttccc cgatcgcctc
     2461 gcgcagcgac cccggaaccc ggggcagccc ttccgctttg taggcgtccc cctcgaagag
     2521 ggccggcggt tcaaccttcc gctccactcc ccagaggccg gccgcgatcg tggcggaaaa
     2581 cgccaggtag gggttcgcgt cggcacccgg gatccggttc tccacccgga ggctctggcc
     2641 ctcgcccacg acgcggaacc cgcacgtgcg gttgtcatgc ccccacaccg ccaggaccgg
     2701 cgcgaacgtc cccgccagat accgcttgta cgagttgatg tacggcgcga agagataggc
     2761 gagctgacgc gcgccggcca gctggccgcc gaggtagtgg cggaacacgc tcgacatccc
     2821 gagcggcgtc ctcccttctt cccagaagag gctgcgcttg ccgtcccggt cccacaggct
     2881 gctgtgcagg tggaacgagg acccggccag gtcggtccgc cactttgcca tgaacgtgag
     2941 cgcgaccccg ttgagcgcgg cgatctcctt ggccgcgtgc ttatagatcg tgtgccggtc
     3001 ggccatttcc accggggtcg tgtaccggag gttgatctcg tgctgcccgg gtccccactc
     3061 gcccttgctg aactcgcagg ggaccccggc ggcctccagg ccgttgcgaa tctggcggac
     3121 cagccactcc tctttggtcc cctggaggat gtggtagtcc tcgatgtact ggccgtacgg
     3181 ttcgaggtcg tgatagtgct tgcggcgggc cccttcgtac gtctcgcgga acaggtagaa
     3241 ctccagctcc gaggcgacca tcgggagcac gccccgctcc tgggcctgct caacctggcg
     3301 gcgcaggatc tggcggggcg actccgggac cggctggccg gcttcgtcgc agacgtcgca
     3361 gagcaccagg gcggtctttt ccagccacgg cagacgccgc agggtcccga ggtcaggcct
     3421 gagccacacg tcctggtacc ccgtcgccca gctcgccagg cggtaccccg gcaggggctc
     3481 catctcgatg tcgcacgcca ggagatagct gcaggcgtgc gcgccgtccc gggccaccgc
     3541 atcgatgaag aaccgcccgg tgacgcgctt ccccaccagc cggccctgca tgtccaccat
     3601 cgcggcgatc accgtgtcga tctcgccggc acccacctgc tcctggagtg cctcgagcgt
     3661 cagccggcct gcggctggct ccgtcatgcc cgctcctccc ctcgtcaccg ggctcccctc
     3721 gatgggcgtt ctctttgctg cggacccgaa cgagacctct ccagggagcc ggtgtcggcc
     3781 cggcgaacca ccccggaggg cggacgatgg ctgcgatggg cgtgatcaac ggcgagcgcc
     3841 tctggggctc gctgatgtcg atggcggaac ttggacgcac ccccggggga ggggtgcgcc
     3901 ggctcgcgct gagcgatgag gaccgccggg cccgcgacct cctggtgcgg tggctgcagg
     3961 aggccgggtg cacggtgcgg gtcgacgatc tcggcaacat ctacgggcgc cgggacggcg
     4021 aggacccaga cgcggcgccg gtcgtgtgcg ggtcccacct cgatacggtg ccgaccggtg
     4081 gccggttcga cggggcgctg ggggtcctgg ccgcgctcga ggcggtccgc acgatgcacg
     4141 atgcgggcct gcggacgcgg cgtgccgtcg aggtcgtctg ctggaccaac gaggaaggcg
     4201 tccgcttcgc gcccgcgatg ctggccagcg gggtggtgtg cggccggttc acccgcgagg
     4261 aggcctacgc cgcgcaggat caggacggcc ggacgttcga ggcggagttg cgccgcatcg
     4321 gctacctggg cgggtccgag caccggatgc gttccccggc cgcgtacatc gagttgcacg
     4381 tcgagcaggg ccccatcttg gagaacgagg gcgcgcaggt cggggtcgtc gaaggcgtgg
     4441 aggggattgc ctgggggtgg gtggacgtga ccggacgggc ggcccacgca ggcccgaccc
     4501 c
//
LOCUS       DASQGH010000544         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33551, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000544 DASQGH010000000
VERSION     DASQGH010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33551"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1652
                     /locus_tag="VEW91_10730"
     CDS             18..>1652
                     /locus_tag="VEW91_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HYM92096.1"
                     /translation="MGRLAEVRERQVPWYRWGPYLSQRQWGTVREDYSPGGAAWEYFP
                     HDHARSRAYRWGEDGLLGICDNHAFLCFAAALWNEADPILKERVFGLSGPEGNHGEDV
                     KEYYYFLDCTPSHSYMRGLYKYPQRAFPYEALVAENRRRGRNRPEYELLDTGIFEGGR
                     YFDIVVEYAKADPEDILIRITATNRGAEPAPLHVLPTLWFRNTWAWGYDGRRPELRAV
                     EPGPGTRRGAAPGFRVLKATHPRLGAYWLACRGAPGLLFTENETNAQRLWRVPNRTAV
                     VKDGIHCAVVGGRADAVSLTPTGTKAAAHYPVRIAAGASQRIELRLSSRPPARPFDDA
                     EAIFSQRQAEADAFYRRLGPEDMPEDARGVQRQAFAGLLWSKQFYCYDVAAWLDGDPA
                     GPAPPEARRRGRNSGWRHLSNEDVISMPDTWEFPWYAAWDLAFQCIPMALVDPDFAKR
                     QLIMLLREWYMHPNGQLPAYEWAFADVNPPVHAWAAWRVYKIARRVTGTADRAFLERV
                     FHKLLLNFTWWVNRQDPEGRNVFQGGFLGLDNIGVFD"
BASE COUNT          252 a          567 c          579 g          254 t
ORIGIN      
        1 tgcaggggga cgccgaactg gggaggctgg ccgaggtccg cgagcggcag gtcccctggt
       61 accgctgggg gccgtacctg tcgcagcgcc agtgggggac cgtgcgcgaa gactactcgc
      121 ccggcggcgc cgcctgggaa tacttcccgc acgaccacgc gcgctcccgc gcgtaccgct
      181 ggggcgagga cggcctcctg ggcatctgcg acaaccacgc ctttctctgc ttcgccgcgg
      241 cgctgtggaa cgaggccgac ccgatcctca aggagcgggt gttcgggttg agcggtcccg
      301 agggcaacca cggcgaggac gtgaaggagt actactactt cctcgactgc acgccgtcgc
      361 actcgtacat gagggggctc tacaagtatc cccagcgcgc ctttccgtac gaggccctgg
      421 tggcggagaa ccggcggcgc gggcggaacc ggcccgagta cgagcttttg gatacgggga
      481 tctttgaggg gggccggtac ttcgatatcg tcgtcgagta cgcgaaagcc gaccccgaag
      541 acatcctgat ccgcatcacc gcgaccaacc ggggggccga gcccgccccc ctgcacgtgc
      601 tcccgaccct ctggtttcga aatacctggg cgtggggata cgatgggcgc cggcccgagt
      661 tgcgcgcggt cgaacccggt ccggggacac gccgaggcgc cgcccccggg ttccgcgtgc
      721 tcaaagcgac ccacccccgg ctgggcgcgt actggctcgc ctgccggggc gcacccgggc
      781 tgctcttcac cgagaacgag acgaacgcgc agcggctgtg gcgcgtcccg aaccggacgg
      841 cggtcgtcaa ggatggcatc cactgtgccg tcgtgggggg ccgcgcagac gccgtgagtc
      901 tcaccccgac cggcacgaag gcggcggccc actacccggt ccggattgcc gcgggggcgt
      961 cccagcgcat tgagctgcgg ctgtccagcc gcccgcccgc gcgtccgttc gatgacgccg
     1021 aggcgatctt ttcgcagcgt caggccgagg ccgacgcgtt ctaccggcgc ttggggccgg
     1081 aggacatgcc cgaggacgcc cggggggtgc agcggcaggc gttcgccggg ctactgtgga
     1141 gcaaacagtt ctactgctac gacgtcgcgg cgtggctgga cggcgacccg gcgggacccg
     1201 cgccgcccga agcgcgcagg cggggacgca actcggggtg gcggcacctc agcaacgagg
     1261 acgtgatctc gatgcccgac acctgggagt ttccctggta cgccgcctgg gatctggcct
     1321 ttcagtgcat cccaatggcg ctcgtcgatc cggactttgc caagcggcaa ctcatcatgc
     1381 tcctccgcga gtggtacatg cacccgaacg ggcagctccc ggcgtatgaa tgggccttcg
     1441 cggatgtgaa ccctccggtg cacgcctggg cggcttggcg cgtctacaag atcgcccggc
     1501 gcgtcaccgg gaccgccgat cgcgccttcc tggagcgcgt cttccacaag ctgctgctca
     1561 acttcacctg gtgggtgaat cgtcaggatc ccgaggggcg caacgtcttc cagggaggct
     1621 tcctcggtct cgacaacatc ggggtgttcg ac
//
LOCUS       DASQGH010000545         6084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30832, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000545 DASQGH010000000
VERSION     DASQGH010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30832"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VEW91_10735"
     CDS             <1..871
                     /locus_tag="VEW91_10735"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HYM92097.1"
                     /translation="AWYGLQPINQKVREGGTLIVTSMLPAEELVKFCHAKPHPYRMAA
                     IPAIPSFSGLWVYKDDHTDVRILGALPRVCPELVSLDSIQKAIREEWKDDLKVTSARR
                     SYEQTTTHTVQPGEGNPEVPFKFELPGWTKMREGVTIDGIRLGEPIKFGDQIGGYRPA
                     RNPYFKKYTTRTMRPVIDFDKCIKCTLCWLQCPDSCFDVTPDQTYDLDAEACCGCGVC
                     EAVCPVDNCVTMVNEAAFADNKSQWEMYKQDKQAYQVWVEEKISHRPERSHGFRYRGQ
                     YQEQVAKTGTGGA"
     gene            943..2175
                     /locus_tag="VEW91_10740"
     CDS             943..2175
                     /locus_tag="VEW91_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HYM92098.1"
                     /translation="MDADSATAVRVAAAEQEALISGSEAVAVATKLADVDVLTAYPIR
                     PYDTLMQYVAKQIANGEQDAEYIVAESEHSQFEIAKHAEAVGARAMCGSSGVGWCYAF
                     EAIAVTPALRLPLLAMVGNRALDDPGAFGTEHNDALAARDLGWLHVWVDTAQEALDTT
                     LIAYRVAEDRRVFLPCAISTDGAFLTHSQALVKIPPKEWVGEFLPPYNRGDLRLHPDN
                     PITIAPQVNEDWLMEIRRQTDQAMRNARGVIEEAYRDFRRIFKRGEGSPFFEEYRTED
                     AEVVLVGMGTLSMPVKVVIRKLREQGQRVGFVRIRWFRPFDYERLGALLSRFGAVGVI
                     DRDFSMGSPLNSGVLANEIRATLYNQDRRPPVVSFIAGLGGREVTIPGVREMIEQTRR
                     AAEAGKAPPDTQWIGVRA"
     gene            2189..3226
                     /locus_tag="VEW91_10745"
     CDS             2189..3226
                     /locus_tag="VEW91_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM92099.1"
                     /translation="MDTKHPTVTLDAPLPVLEPIKGVKKAPLEEYFTSGHRTCQGCES
                     ALVMKLMVKAAGPRTVVLGSTGCMYVANTTYYSTSWVVPWMHTQLGSSGSAAVGTAAG
                     FRALMRKGKIKREPINVISFCGDGGGADMGLSAISGALQHMDYNHLILLYDNESYANT
                     DIQASGSTPYGANTTFSPPGKAKRILHKRWKKNMAAMLAAGHPECRYVGTVCASYAVD
                     FMNRIRKALSIGGPTFIHSLDPCPKGWDYDPMLSHELGELAVLTGIWPLFEVEQHRLR
                     MYGKTKAIAEGRQKRLPVRDYLLKQGRFAHFTDDDINYFQAKIDEMWTKWLIPGVLPF
                     STEVMNDSPPA"
     gene            3442..4752
                     /gene="oxlT"
                     /locus_tag="VEW91_10750"
     CDS             3442..4752
                     /gene="oxlT"
                     /locus_tag="VEW91_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HYM92100.1"
                     /translation="MATVAPDKTQERLLRSRWWILAAAILAQIPNANLQYAWTLFPSH
                     IVSDGLGKLSKVQETFALFVLLETWLVPFEGWLVDRIGPRLLTAVGGALIGISWVACS
                     TVHSLGGLLFWYGIVGGIGGGIIYGATIATALKWFPDHRGLTAGLVAAGFGAGAALSV
                     APIDSVISHAGWRAAFFQFGIIQGIIVILAALFLSAPPEGFKIAAIAKRVSRHIHQAA
                     VDSTPAEMIRTPHFWVMYVMMVLVGGGGLMATAQLGPIARSTGVDRVIVLWGLTALVL
                     ALQIDRVLNGITRPIWGWISDHIGRANSMFIAFGIQGFAILWLLSLLKHPIGFIVASG
                     AAFFFWGEMYSLFPALVGDLWGRKYAATNYGLMYTAKGVASIWAGPVAAYMFETQKTW
                     TTVFYLAAAANFIASAMALLVLRRMSVPKAEAQARMAPAAGGGG"
     gene            5054..5926
                     /locus_tag="VEW91_10755"
     CDS             5054..5926
                     /locus_tag="VEW91_10755"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYM92101.1"
                     /translation="MAEKYAHPEVLVETGWLSEHLKDPAIRVVEVSEDVTLYGQGHIP
                     GAVHFNWKSHLQDPVRRDWISLQEFETLLGGHGVGPGTTLVLYGDKNNWFATYTFWLS
                     KLYGVERVTILNGGRGKWIAEGRPMTTEVPAYPKVTFRARAADPNIRAFRDQVLERLG
                     KPQVALVDVRSPQEYSGELIAMPAYPQEGAQRGGHIPTAQSIPWVQNVREDGTFKAPE
                     ELRALYQGKGITPDKEVIAYCRIGERSSHTWFTLKYLLGYPTVRNYDGSWTEWGSLVG
                     VPIENPSAAEATRR"
BASE COUNT         1027 a         1927 c         2188 g          942 t
ORIGIN      
        1 ggcctggtac ggcctgcagc ccattaacca gaaggtccgc gagggtggga ccctgatcgt
       61 cacctcgatg ctccccgccg aggagctcgt caaattctgc catgcgaagc cccaccccta
      121 tcggatggcg gcgatcccgg cgatcccgag cttctcgggg ctgtgggtgt acaaggacga
      181 tcacaccgat gtccggatcc tgggcgcgct gcccagggtc tgccccgaac tggtcagcct
      241 ggacagcatc cagaaagcga tccgggagga atggaaggac gatctgaagg tcacctccgc
      301 ccgccggtcg tacgagcaga cgaccacgca cacggtgcag ccgggggaag gcaaccccga
      361 ggtcccgttc aagtttgagc tccccggctg gacgaagatg cgggagggcg tgacgatcga
      421 cggcatccgg ctcggcgagc cgatcaagtt tggggaccag atcggcgggt atcgcccggc
      481 gcgcaacccg tacttcaaga agtacacgac ccggacgatg cggccggtca tcgacttcga
      541 caagtgcatc aagtgcacgc tgtgctggct gcagtgtccc gactcgtgct tcgacgtgac
      601 gcccgaccag acgtacgacc tggacgcgga ggcctgctgc ggctgcggcg tctgcgaggc
      661 ggtgtgcccg gtcgataact gcgtcacgat ggtcaacgag gccgcgtttg ccgacaacaa
      721 gagccagtgg gagatgtaca agcaagacaa gcaggcgtac caggtctggg tcgaggagaa
      781 gatcagccac cggcctgagc gctcccacgg gttccgctac cgggggcagt accaggagca
      841 ggtcgcgaag acggggaccg gcggggccta ggccgagaag aaggggccga cccgggcggg
      901 caagaggaga tgggtgttgg cgcataaagg ggggaaggcg tgatggatgc cgattcggcg
      961 accgcggtcc gggtcgcagc agcggagcag gaggcgctga tcagcgggag cgaggcggtc
     1021 gcggtcgcga ccaagctggc cgacgtcgac gtcctcaccg cgtacccgat ccggccgtac
     1081 gacacgttga tgcagtacgt ggccaagcag atcgccaacg gcgagcagga cgccgagtac
     1141 atcgtggcgg agtccgagca cagccagttc gagatcgcca agcacgcgga ggcggtgggc
     1201 gcgcgcgcga tgtgcggctc gagcggggta gggtggtgct acgcgttcga ggcgatcgcc
     1261 gtcaccccgg ccctccggct gcccctgctg gccatggtgg gcaaccgggc gctggacgac
     1321 cccggggcgt tcggcacgga gcacaacgac gcgctcgcgg cccgcgatct cgggtggctg
     1381 cacgtctggg tcgatactgc ccaggaggcg ctcgacacca cgctgatcgc ctaccgggtg
     1441 gcggaggatc gccgggtctt cctcccctgc gcgatcagca ccgacggggc gttcctgacg
     1501 cactcgcagg cgctggtcaa gatcccgccc aaggagtggg tcggtgagtt tctccccccg
     1561 tacaatcgcg gggatctccg gctccacccc gacaacccga tcaccatcgc cccgcaggtc
     1621 aacgaggact ggctgatgga gatccggcgc cagaccgacc aggcgatgcg gaacgcccgg
     1681 ggcgtgatcg aggaggcgta ccgggacttc cgccggatct tcaagcgggg cgagggcagc
     1741 ccgttcttcg aggagtaccg caccgaggac gccgaggtcg tgctggtcgg catggggacg
     1801 ctctcgatgc cggtgaaggt cgtcatccgc aagctccgcg agcaggggca gcgggtgggg
     1861 ttcgtgcgga tccggtggtt ccggccgttc gactacgagc ggctcggcgc gctcctctcg
     1921 cggtttgggg cggtcggggt catcgaccgg gacttttcga tgggctcgcc gctcaacagc
     1981 ggggtcctgg ccaacgagat ccgggcgacg ctctataacc aggaccggcg cccgcccgtg
     2041 gtgagcttca tcgccgggct cggcgggcgg gaagtcacga ttcccggcgt gcgtgagatg
     2101 atcgagcaga cccgcagggc tgccgaggcc gggaaggccc cgccggacac ccagtggatc
     2161 ggcgtgagag cgtaggcgag gaggagcgat ggacacgaag catcccaccg taacgctgga
     2221 cgcaccgctc ccggtcctgg agccgatcaa gggcgtcaag aaggcgcccc tggaggagta
     2281 cttcacctcc ggccaccgga cgtgccaggg gtgcgaatcg gcgctcgtga tgaagctcat
     2341 ggtgaaggcg gcgggacccc gcacggtcgt gctcgggagc accgggtgca tgtacgtcgc
     2401 caacaccacc tactacagca cgtcctgggt cgtgccctgg atgcacaccc aactcggttc
     2461 gtcgggctcg gcggcggtcg gaacggccgc gggcttccgg gcgctcatgc ggaagggcaa
     2521 gatcaagcgc gagccgatca acgtgatctc gttctgcggc gacggcggcg gggcggacat
     2581 ggggttgtcc gcgatctccg gcgccctgca gcacatggac tacaaccacc tgatcctcct
     2641 gtacgacaac gaatcgtacg ccaacaccga catccaggcc tccggcagca cgccgtacgg
     2701 ggccaacacc acgttcagcc cccccggtaa ggccaagcgg atcctccaca agcggtggaa
     2761 gaagaacatg gccgcgatgc tcgccgcggg gcatccggag tgccggtacg tcggcacggt
     2821 gtgcgccagc tatgccgtgg acttcatgaa ccggatccgg aaggcgctca gcatcggcgg
     2881 cccgaccttc atccattccc tcgatccctg ccccaagggg tgggattacg accccatgct
     2941 ctcccacgaa ctgggcgagc ttgcggtgct gaccgggatc tggcccctgt tcgaggtcga
     3001 gcagcaccgg ctgcgcatgt acgggaagac caaggcgatc gcggagggcc gccagaagcg
     3061 gctcccggtc cgggattatc tactgaagca gggccggttc gcccacttca ccgatgacga
     3121 catcaactac ttccaggcga agatcgacga gatgtggacg aagtggctga tccccggcgt
     3181 gctgccgttt tccaccgaag tcatgaacga cagccccccc gcctgacgcg gggcgccccc
     3241 cgggggatgg ccgaggccgg gggcacaact gcatagaact gcatagcacg agccgcgtgg
     3301 cttcgcgatc acggaagtgc ggggtgacgg tgcggtgtgg cgctgaaggg cttcctcttg
     3361 acggcagctc aggcacctgc gaggggaagc cctttgtccg tgtgcgcaaa ctcgtaggag
     3421 cagtcatcgg gggtgagcgg catggcgacg gtggcgccgg ataagacgca ggagcggctg
     3481 ttgcggtcgc ggtggtggat cctggcggcc gcgatcctcg cccagatccc caacgccaac
     3541 ctccagtacg cgtggacgct cttcccatcg catatcgtca gcgacgggtt gggcaagctg
     3601 agcaaggtcc aggagacgtt cgcgctgttc gtgctcctgg agacctggct cgtgccgttc
     3661 gaggggtggc tggtggatcg catcgggcca cgcctgctca ccgcggtcgg tggcgcgctg
     3721 atcgggatca gctgggtggc gtgctcgacc gtccattccc tcggcggctt gctcttttgg
     3781 tacggcatcg tcggtgggat cggcggcggg atcatctacg gcgccacgat cgcgacggcg
     3841 ttgaagtggt tcccagacca tcggggcctg accgcgggcc tggtggcggc ggggtttggg
     3901 gccggcgccg cgctgtccgt ggcgccgatc gacagcgtca tcagccacgc gggatggcgg
     3961 gcggccttct tccagttcgg catcatccag gggatcatcg tgatcctcgc agcactgttc
     4021 ctgagcgcgc cgcccgaggg gttcaagatc gccgccatcg cgaagcgggt gtcccgacac
     4081 atccaccagg ccgcggtcga cagcacgccc gcggagatga tccgcacccc ccacttctgg
     4141 gtcatgtacg tgatgatggt cctggtcggc ggcggcgggc tcatggcgac ggcccaactc
     4201 gggccgatcg ccaggtcgac cggcgtggac agggtgatcg tcctctgggg gctcaccgcg
     4261 ctggtgctgg cgttgcagat cgaccgcgtg ctcaacggga tcacccggcc gatctggggg
     4321 tggatctccg accatatcgg ccgggccaac tcgatgttta tcgcgttcgg cattcaggga
     4381 ttcgcgatcc tctggctgct ctcactgctg aagcatccga tcgggttcat cgtggcgtcg
     4441 ggcgccgcgt ttttcttctg gggcgagatg tattcgctgt tcccggccct ggtgggagac
     4501 ctgtggggcc ggaagtacgc cgccaccaac tacggtctca tgtacaccgc gaagggggtg
     4561 gcctcgatct gggccggccc ggtggcggcg tacatgtttg agacgcagaa aacctggacc
     4621 accgtgttct acctggctgc ggccgcgaac ttcatcgcgt ccgccatggc gctgttggtg
     4681 ctcaggcgga tgtcggtgcc caaggcggag gctcaggcgc ggatggcccc cgcggccggc
     4741 gggggagggt agcgcacgat cttgacaggg gctttcgacg cgtgttagaa ggggaacgat
     4801 gagcaccggg cacgcgccgc accgtcccca tcaatgcatc tgtccccgcg gaggccgggc
     4861 tcgccttcgc ccaaagcacg ttgatgctgc cggcgctctc cttggcgaag tcctctaacg
     4921 catagcctgg catccgcgcc gcgacgggta gtgccccgcg cccgctgcgc ccgtttgggg
     4981 cgggtgcagg gtggactcga gaccgaacga gactcaggga tctgagacac gaaccatcag
     5041 ggaggggaga ccgatggctg agaaatatgc gcaccccgag gtgctggtgg agacggggtg
     5101 gctgagcgag cacctgaagg accccgcgat ccgtgtggtc gaggtctcgg aggacgtgac
     5161 cctgtacggg caggggcaca tccccggggc ggtacacttc aactggaaga gccatctcca
     5221 agacccggtg cgccgcgact ggatctccct gcaggagttc gagacgctgc tcggggggca
     5281 cggggtgggc ccgggcacga cgctcgtgct gtacggggac aagaacaact ggttcgccac
     5341 ctacacgttc tggctgagca aactgtacgg ggtcgagcgt gtcacgatcc tcaacggggg
     5401 ccgggggaag tggatcgccg aaggccggcc gatgacgacc gaggtgccgg cctaccccaa
     5461 ggtgacgttc cgggcccggg cggccgatcc gaacatccgg gcgttccggg accaggtcct
     5521 cgagcgcctg gggaagcccc aggtggcgct ggtcgacgtc cggtccccgc aggagtactc
     5581 gggcgagttg atcgcgatgc cggcgtaccc ccaggagggg gcgcagcggg gcgggcacat
     5641 tcccaccgcg cagagcatcc cgtgggtcca gaacgtccgg gaggacggga cgttcaaggc
     5701 cccggaggag ctgcgggcgc tgtaccaggg caaggggatc accccggaca aggaggtcat
     5761 cgcctactgc cggatcgggg agcgctcctc gcacacctgg ttcacgctca agtacctgct
     5821 ggggtatccc accgtgcgca actacgacgg gtcgtggacg gagtggggca gtctcgtggg
     5881 cgtgccgatc gaaaacccct cggccgccga agcgacccgg cggtagggga ccgcggcggc
     5941 gcggccggat cgtgccgggc gtcgccggat ggtggtttga cggtatcgcc agatgcgtgc
     6001 tagaatgacc ggcgagacga tgaaggctcc cccgcccggc ggccggtcgg gctagtcggc
     6061 gcgggcccgg gtccggccgg actg
//
LOCUS       DASQGH010000546         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16510, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000546 DASQGH010000000
VERSION     DASQGH010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16510"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VEW91_10760"
     CDS             complement(<1..910)
                     /locus_tag="VEW91_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HYM92102.1"
                     /translation="MYTIVNYLNQFAGGVGGEKSASLPPKWVDGAVGPGKLLQAALAP
                     EARVAGTIVCGDDYVAAHSAGALAAILSYVREAGADALVAGPAFGAGRYGVACSTVCE
                     ACREQLKIPAITAMHRENPGVEVARPTLIVPTADTALDMPEAVRTLARLALKLCRGEA
                     LGPAAQEGYLPTGVKKNIRVPLNGAQRATAMLLKKIAGVPFQTEIEVPRVDDVPPASP
                     LDDLAHATVALVSSGGIVPKGNPDRLESRRATKWLKYSIAGLTDLSPDEFECIHGGFD
                     GTATNKDPDRVTPIDALRVLERNGEIG"
     gene            complement(1157..2434)
                     /locus_tag="VEW91_10765"
     CDS             complement(1157..2434)
                     /locus_tag="VEW91_10765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYM92103.1"
                     /translation="MSKRAGVRLAVQGGTPVRTEPLLPGYPGGLLIGEEEKAAVLEVL
                     DSQSLFRHYGPRPLHKVAQFERAFALAMGARHAVGVSSGTAALMTALAALGAGPGDEV
                     IVPTYTWVATINAVVSLGAVPVFVDIDDTLTLDPSKIDAAVTSSTKALLPVHMRGAGA
                     DMAPILDAARRHGLRVVEDAAQAVGGRYRGRRLGTLGDLGAYSLQYHKIITTGEGGMV
                     VTDDTKLWERAVRYHDQGSARMEELDETIPVGNPLMIGINFRMGEITGAIGLVQLGRM
                     DWIIDRMRAHKAAIIDGLAGTGGLTVRRIPDPDGDTGATLIFFLPSADTARQFSTALA
                     AEGVRNMVAWDSGQHVYYHFDQIIERRMFAERHCAWECPHYKGTARLAKGMFPQTDDL
                     LRRAIHIDLNPLLTERDEADIVRAVRKVATALL"
BASE COUNT          365 a          857 c          820 g          407 t
ORIGIN      
        1 ggccgatctc cccgttccgc tcgagcaccc gcagcgcgtc gatgggggtc acacggtccg
       61 ggtccttgtt ggtcgcggtg ccgtcgaacc ctccatggat gcattcaaac tcgtcggggc
      121 tgagatccgt taggccggca atggagtact tcaaccactt cgtggcgcgc cgcgattcca
      181 gccgatcggg gttccccttg gggacgatcc ctcccgacga caccagggcg accgtcgcgt
      241 gcgccaagtc gtcgagcgga gacgccggag gcacatcgtc gacccgcggc acctcgattt
      301 ccgtctggaa cggaacgccg gcgatcttct tgagcagcat ggcggtggcc cgctgggcgc
      361 cgttgagggg cacgcggatg ttcttcttga cgccggtcgg caagtagccc tcctgggcgg
      421 ccggcccgag cgcctcgcct ctgcacaact tgagggcgag cctcgccagc gtccggacgg
      481 cctccggcat gtcgagcgcg gtgtcggcgg tcggcacgat cagggtcggc cgggccacct
      541 ccaccccggg attctcgcga tgcatcgcgg tgatcgccgg gatcttcagt tgctcgcggc
      601 acgcctcaca gacggtgctg caggcgacgc cgtaccgccc ggcgccgaag gccggccccg
      661 cgacgagggc gtccgccccg gcctcacgga catatgagag gatcgcggcc agcgcgccgg
      721 cgctgtgcgc cgccacgtaa tcgtcaccgc acacgatcgt cccggcgacc cgcgcctcgg
      781 gcgccagcgc cgcttgtaga agtttcccgg gtcccacggc gccgtcgacc cacttcggcg
      841 ggaggctggc gctcttctca ccgccgacgc ctcccgcaaa ctggtttagg tagttgacga
      901 tcgtgtacat gcgcgccggc cccctcttcg tcacatgccc gaggacctct cccgacgtgc
      961 taaccggtcc aaccgcagtg cctcgccaca tagtggaacg ccgtgtccag ggaatcgggt
     1021 gcggtacacc gcgaggcagt acttgtaggg gttccagcaa acgcttcgtc gtgccagcga
     1081 atgccgttga ggtgagcccg ggcgacgcct ccgtacccga gcagtccgac gccaaggacg
     1141 ctcgacaccg aaggtcctac aagagcgcgg tcgccacttt gcgaaccgcg cgcacgatgt
     1201 cggcctcgtc gcgctcggtc aagagcgggt tgaggtcgat atggatcgcg cgccgcagga
     1261 gatcgtcggt ctgggggaac atgcccttcg cgaggcgggc ggtgcctttg tagtgggggc
     1321 actcccaggc gcagtgacgc tccgcgaaca tccggcgctc gatgatctgg tcaaagtggt
     1381 agtagacgtg ctgcccgctg tcccacgcca ccatgttccg cacgccttct gcggccagcg
     1441 ccgtgctgaa ctgccgggcc gtgtcggcgg agggaaggaa gaagatcagg gtggcgccgg
     1501 tgtcgccgtc gggatcgggg atgcggcgga cggtgagccc gccggtgccg gcgagcccat
     1561 cgatgatcgc cgccttgtgc gcccgcatcc ggtcgatgat ccagtccatc cggccgagct
     1621 gcaccaggcc gatcgccccg gtgatctcac ccatccggaa gttgatgccg atcatcagcg
     1681 ggtttcccac cggtatcgtc tcgtcgagct cctccatgcg ggccgaccct tggtcatggt
     1741 accggacggc ccgctcccac agtttggtgt cgtcggtcac gaccatcccg ccctcgccgg
     1801 tcgtgatgat cttgtggtac tggaggctgt aagcgcccag gtcgcccagg gtgcccaacc
     1861 gccggcctcg ataccggccg cctacggcct gcgccgcgtc ctcgaccacg cggagcccat
     1921 ggcgccttgc cgcgtcgagg atcggggcca tgtcggcgcc ggcgccgcgc atgtgcaccg
     1981 gcaggagagc tttggtgctg gatgtcacgg cggcgtcgat cttcgaggga tccaacgtca
     2041 gcgtgtcgtc gatgtcgaca aacacgggca cggcgcccaa ggagaccacg gcattgatcg
     2101 tggcgaccca ggtgtaggtc ggcacgatca cttcgtcgcc gggacctgcg ccgagggcgg
     2161 cgagcgccgt catcagtgcc gcggtgccgg agctcacccc caccgcgtga cgagcgccca
     2221 tcgccagggc aaacgcccgc tcgaactgcg ccaccttatg gagcgggcgg gggccgtaat
     2281 ggcggaagag actctggctg tcgagcactt ccaggaccgc ggccttctcc tcttcgccga
     2341 tgagcaaccc cccagggtat ccgggtagca gaggctcggt gcgcaccggc gtgccccctt
     2401 gcaccgcaag ccgtacccct gcgcgcttgc tcatggtctc cctcctgtg
//
LOCUS       DASQGH010000547         5959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47800, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000547 DASQGH010000000
VERSION     DASQGH010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47800"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /gene="rpsM"
                     /locus_tag="VEW91_10770"
     CDS             <1..251
                     /gene="rpsM"
                     /locus_tag="VEW91_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYM92104.1"
                     /translation="VTRLREVIDRNYKVEGDLRRDVAMDIRRLVEIGSYRGQRHRKGL
                     PVRGQRTRTNARTRKGPRKTVGRVRRAPAAAGAAPATT"
     gene            261..647
                     /gene="rpsK"
                     /locus_tag="VEW91_10775"
     CDS             261..647
                     /gene="rpsK"
                     /locus_tag="VEW91_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYM92105.1"
                     /translation="MAKGARGKRKEKKNVPAGIAHIQSTFNNTVVTISDLQGNVVAWA
                     SAGTSGFKGSRKGTPFAAGLAAENAARKAMEQGVRQVEVYVKGPGAGREAAIRSLQAA
                     GLEVNLIKDVTPIPHDGCRPPKRRRV"
     gene            666..1292
                     /gene="rpsD"
                     /locus_tag="VEW91_10780"
     CDS             666..1292
                     /gene="rpsD"
                     /locus_tag="VEW91_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYM92106.1"
                     /translation="MGRYHDAVCRLCRREGMKLYLKGEKCYTDKCPVAKDATPPGMHG
                     PSRRKPSDFAVRLREKQRARRFYGAFEGQFKRYFKSAARIKGITGTQLLQLLETRLDN
                     VVYRLGLGASRKAARQLVAHGHIAVNGRRVTVPSAQVRPGATVGVAAGSRDQARFKAL
                     QAAAPAHGLPRWLEYSPERLEGRVLTLPAREEIDVPVREQLIVEYYSR"
     gene            1362..2297
                     /locus_tag="VEW91_10785"
     CDS             1362..2297
                     /locus_tag="VEW91_10785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYM92107.1"
                     /translation="MWDTTKPKIEYAELSDTYGKFIVEPLERGFGVTLGNALRRVLLS
                     SISGAAVTSVKIENVLHEFSTIPGVVEDVTQVILNLKELSLKLHSDKPKLLRMDVRGK
                     KDVVAGDLQPDAEVEVLNPDLHIATLDGKTAHLAMELVVERGKGYVPAERHRKSEHVI
                     GVIPVDSVFSPIQKVNYAVEDTRVGHAADYDRLVLEVWTDGSIRPEEALQESARLLID
                     CFRLFVGAAAAVEVPVGPQADEASKMAAMPIEELDLSVRPYNCLKRAGINTLGELLQR
                     TEEEVVNVKNFGRKSLDEVKEKLAALGLELRRRGA"
     gene            2300..2650
                     /gene="rplQ"
                     /locus_tag="VEW91_10790"
     CDS             2300..2650
                     /gene="rplQ"
                     /locus_tag="VEW91_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HYM92108.1"
                     /translation="MLGQRGRRLGRDTGARLALFRGLVSALIVEERITTTEHKAKEAK
                     KVADRVIDLALRNDLQARRQVARLLLDRAVVRRLFGTVAPRYQKGRGGYTRIVRKVPR
                     RGDAAPMALLELVK"
     gene            2685..3527
                     /locus_tag="VEW91_10795"
     CDS             2685..3527
                     /locus_tag="VEW91_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HYM92109.1"
                     /translation="MVPPLITVRDLHFVYHAGTPAATPALAGLDLDIAPGECLAIIGG
                     NGSGKSTLAKHLNALLVPTSGEVRVEGVDTRDPDGAWLARQRVGMVFEHPDNQIVAAI
                     VEEDVAFGCENLGLPPAEIRARVDRALLAVGLERLRRHPPHQLSGGQKQRLAIAGVLA
                     MQPRCLVLDEATSMLDPQGHQEVMETALTLCRRERLALVLITHAMEDAARADRVVVLA
                     GGRIALAGPPAEVFAREDELRRLRLEPPELARLSRALAAAGLAIPRDVLTIDALVAAL
                     TPGP"
     gene            3524..4390
                     /locus_tag="VEW91_10800"
     CDS             3524..4390
                     /locus_tag="VEW91_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HYM92110.1"
                     /translation="MIRCEHVTYVYGRGTPLAVPALIDVSLQISAGEVVGIIGATGSG
                     KSTLIQHFNGLLRPTSGRVLLDDVDIHAPRVDRRRIRQQIGLLFQYPEQQLFEETVFA
                     DVAFGPRNLGLPADEVRARVHQALEHVGLPPDQVGPRSPFALSGGEMRRAAIAGVLAM
                     DPRMLILDEPTAGLGPRARRDLLEHIRRLRRERALTIVLVTHSMDAVAQLCERLLVLD
                     RGRLVADGPIRTVFADPARLTALGLGLPQVTLLAHRLRERGLPVRPDVLTVEEARVAI
                     LKALGVAGRGIL"
     gene            4377..5198
                     /locus_tag="VEW91_10805"
     CDS             4377..5198
                     /locus_tag="VEW91_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HYM92111.1"
                     /translation="MEFFRGLVLGQYVPVDSVVHRLDPRTKILATLVLLAVAFAIREF
                     AGLAVLTLGLAAVVLAARVPPGYLLRGMRPLFWLLAFAVVMQVFFGEGGGHPVVRWGP
                     VVVTRENLSQAAFYGWRLILVVLSTTVMTVVTSPMEFTDGMERLLRPFQRVGVPAHDL
                     AMMMTIALRFIPTLLEEAEKIMKAQMARGAEFTRGSFPARARALTPLLVPLFISAFRR
                     ADALAVAMEARCYRGGEGRTRMNVLWFRPRDAIAFTLVTAAAALALLPPAAWRGR"
     gene            5202..>5959
                     /gene="truA"
                     /locus_tag="VEW91_10810"
     CDS             5202..>5959
                     /gene="truA"
                     /locus_tag="VEW91_10810"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYM92112.1"
                     /translation="MRTLRLVLEYDGTGYCGWQRQRESAARGLPSVQATLEAALREIT
                     GEATQAVGAGRTDAGVHALGQMAHFTTDSRIPTSAWPPALHGHLPPDIRILGADEVPP
                     GFHARFDAVSRTYRYEILNRPAPSALLRHRAYHVPEPLDVGAMQRALAALVGRHDFAA
                     YRRMGSPSRTTICTVTAAGCARTGSRVRVTITADRFLRHMVRMIVGTLVRVGTGRLPV
                     DAPGAYLADRDSQRTGPTAPAHGLYLVRVEYGPPG"
BASE COUNT          852 a         2044 c         2211 g          852 t
ORIGIN      
        1 aggtcacccg gctgcgcgag gtgatcgacc gcaactacaa ggtcgagggg gacctccgcc
       61 gcgatgtggc gatggacatc cggcggctgg tcgagatcgg ctcgtaccgc ggccagcgcc
      121 accgcaaggg cctcccggtc cgcggccagc gcacgcggac gaacgcgcgg acccggaagg
      181 ggccgcgcaa gacggtgggc agagtccgga gggccccggc ggccgcgggg gccgctccgg
      241 ccacgaccta ggaggcgagc atggccaagg gcgcacgcgg caagcggaag gagaaaaaga
      301 acgttccggc gggcatcgcc catatccagt cgacgttcaa caatacggtc gtgacgatca
      361 gcgacctcca ggggaacgtc gtcgcgtggg cgagcgcggg cacctccggg ttcaaaggct
      421 cccgcaaggg gacgccgttt gcggccggcc tcgccgcgga gaacgcggcg cggaaggcga
      481 tggagcaggg ggtgcgccag gtggaggtat acgtcaaggg gcccggggcc gggcgcgagg
      541 ccgcgatccg gtcgctccag gccgccgggt tggaagtcaa cctgatcaag gatgtgacac
      601 ccatcccgca cgatggatgc cggccgccca agcggcggcg ggtgtaggag gctaaaggag
      661 ggcgcatggg tcggtatcac gacgcggttt gccggttgtg ccgccgggag ggcatgaagc
      721 tctacctcaa gggggagaag tgctacaccg acaagtgccc ggtggccaag gatgccacgc
      781 ctccggggat gcacgggccg agccggcgca agccgtccga tttcgccgtg cgcctccggg
      841 aaaagcagcg ggcgcgccgg ttctatgggg cgtttgaggg gcagttcaag cgctacttca
      901 agtcggcggc gcggatcaag ggcatcacgg ggacccagct gctccagctc ctcgagaccc
      961 ggctggacaa cgtcgtctac cggctcggat tgggcgccag ccgcaaggcg gcccgccagc
     1021 tcgtggccca cgggcacatc gccgtcaacg gccgccgggt cacggtgccg agcgcccagg
     1081 tgcgcccggg ggcgaccgtg ggggtggcgg cgggcagccg cgaccaagcc cgcttcaagg
     1141 cgctccaggc cgccgccccg gcccacggcc tgccccgctg gctggagtac agtcccgagc
     1201 ggctggaggg gcgggtgctg acgctgccgg cccgcgagga gatcgatgtc ccggtccggg
     1261 aacaactgat cgtcgagtac tactcgaggt agccgactat ggagacaaat ctgctggggg
     1321 gggcgacggg gttgcacgtt ccgaacaggg ggttactaga cgtgtgggac accacgaagc
     1381 ccaagatcga gtacgcggag ctctccgata cgtacgggaa gtttatcgtg gagccgctgg
     1441 agcgggggtt cggggtcacc ctggggaacg cgttgcggcg ggtgctgctg tcctcgatct
     1501 cgggcgcggc cgtgacctcg gtgaagatcg agaacgtcct gcacgagttc tcgaccattc
     1561 ccggggtcgt cgaggacgtc acccaggtga tcctcaacct caaggaactg tcgctcaagc
     1621 tgcacagcga caagccgaag cttctacgga tggatgtgcg gggcaagaaa gacgtcgtcg
     1681 cgggcgatct ccagcccgac gccgaggtcg aggtcctcaa ccccgatctg cacatcgcca
     1741 cacttgatgg gaagaccgcc catctggcca tggaactggt cgtggagcgc ggcaagggct
     1801 acgtcccggc cgagcggcac cggaagagcg agcacgtcat cggcgtgatt ccggtcgact
     1861 cggtgttctc gcccattcag aaggtgaact acgcggtgga ggacacccgg gtgggccacg
     1921 cggccgacta cgaccggctc gtgctcgagg tctggaccga tgggagcatc cggccggagg
     1981 aggccctcca ggaatccgcc cggctcctga tcgactgctt ccgcctcttc gtcggggccg
     2041 cggcggcggt ggaggtcccg gtggggccgc aggcggacga ggcgagcaag atggccgcga
     2101 tgccgatcga agagctcgac ctctcggtcc ggccgtacaa ctgcctgaag cgggcgggga
     2161 tcaacaccct gggcgagctg ctgcagcgca cggaagagga agtggtcaac gtcaagaact
     2221 tcggccgcaa atccctcgat gaggtgaagg agaagctcgc cgcgttgggg ctggagctgc
     2281 gccggagggg agcctgatcg tgctggggca gcgggggcgg cggctcgggc gcgacaccgg
     2341 cgcgcggctg gcgctgtttc gcggcctggt gtccgccctc atcgtcgagg agcggatcac
     2401 cacgaccgag cacaaggcca aggaagccaa gaaggtggcc gaccgggtga tcgatctcgc
     2461 gctgcgcaac gatctgcagg cccggcggca ggtcgcccgc ctcctgctcg accgcgcggt
     2521 cgtgcggcgc ctgttcggca ccgtcgcccc gcggtatcaa aaagggcgcg gcgggtacac
     2581 ccggatcgtc cggaaagtcc ccaggcgggg cgatgctgcg ccgatggcct tgctcgagct
     2641 tgtgaagtag ccggcgcgcc ccgcacgggg tgcgcccccc ctcgatggtc cctcccctga
     2701 tcacggttcg ggacctccac ttcgtctacc acgcggggac ccccgccgcg acacctgcgc
     2761 tcgcgggcct cgacctcgac atcgcccccg gcgaatgcct cgccatcatc ggcgggaacg
     2821 gctccggcaa gagcacgctg gccaagcacc tcaacgccct cctggtgccc accagcggcg
     2881 aggtgcgggt ggagggggtc gacacccggg accccgacgg ggcctggctg gcgcgacagc
     2941 gggtcggcat ggtgttcgag cacccggaca accagatcgt cgcggcgatc gtggaagagg
     3001 atgtcgcctt tgggtgcgaa aacctcgggc tgccgcccgc cgagatccgc gcgcgcgtgg
     3061 accgggcgct gctcgcggtc gggctggagc ggctcagacg ccacccgccg caccagctat
     3121 cgggcgggca gaagcagcgg ctggcgatcg ccggggtgct ggcgatgcag ccccggtgcc
     3181 tggtgctgga cgaggccacc tcgatgctgg acccccaggg gcaccaggag gtgatggaga
     3241 cggcactcac gttgtgccgc cgcgaacgcc tggccctggt cctcatcacc cacgccatgg
     3301 aagacgccgc gcgggcggac cgggtcgtcg tgctggccgg ggggcggatc gcgctcgcgg
     3361 ggccgccggc ggaggtcttc gcccgggagg acgagctgcg ccggctccgg ctcgagccgc
     3421 cggaactggc ccgcctgagc cgggccctgg ccgccgccgg cctggccatc ccccgggacg
     3481 tgctgacgat cgacgcgctg gtcgcggcgc tgaccccggg gccatgatcc ggtgcgagca
     3541 cgtgacgtac gtctatgggc gcgggacgcc gctcgcggtg cccgcgctca tcgacgtgtc
     3601 gctacagatc tcggccgggg aggtcgtcgg gatcatcggg gcgaccggct cgggcaagtc
     3661 cacgctgatc cagcacttca atgggttgct ccgccccacg agcgggcggg tcctgctcga
     3721 cgacgtcgac atccatgccc cccgggtcga tcgccggcgc atccgccagc agatcggcct
     3781 cctgtttcag taccccgagc agcagctgtt cgaggagacg gtgttcgcgg atgtggcgtt
     3841 cgggccgcgg aatctgggcc tgcccgccga cgaggtgcgc gcccgggtgc accaggccct
     3901 ggagcatgtc gggctccccc ccgaccaggt gggcccccgc tccccgttcg cgctcagcgg
     3961 gggggagatg cggcgggccg cgatcgccgg ggtgcttgcg atggacccgc ggatgctcat
     4021 cctggacgag cccacggcgg gcctgggtcc ccgggcccgc cgcgaccttc tggagcatat
     4081 ccggaggctc cgccgcgagc gcgcgcttac catcgtgctg gtcacgcaca gcatggatgc
     4141 cgtggcccaa ctctgcgagc gcctgctcgt gctcgatcgg gggcggctgg tggcggacgg
     4201 ccccatccgg accgtgttcg ccgatcccgc ccggctcacc gcgctggggc tgggattgcc
     4261 gcaggtgacc ctgctcgccc accggctccg ggagcgcggc ctccccgtcc ggccggatgt
     4321 cctcaccgtc gaagaagccc gggtcgcgat cctcaaggcg ctcggggtgg ccggccgtgg
     4381 aattctttag ggggctcgtc ctcggccagt atgtgccggt ggactcggtg gtccaccggc
     4441 tcgatccgcg gaccaagatc ctcgcgaccc tcgtgctgct cgccgtcgcg tttgcgatcc
     4501 gggagtttgc cggcctcgcg gtgctgaccc tggggctcgc cgcggtggtc ctggccgcgc
     4561 gggtcccccc cgggtatctc ctccggggga tgcggccctt gttctggctc ctcgccttcg
     4621 ccgtcgtgat gcaggtgttc ttcggcgagg ggggcggcca cccggtggtc cggtgggggc
     4681 cggtggtggt cacccgcgag aacctctccc aggcggcgtt ctacggctgg cggctgatcc
     4741 tggtggtgct gtccacgacc gtgatgaccg tcgtgacctc gccgatggag ttcaccgacg
     4801 ggatggagcg cctcctccgt ccgtttcagc gggtcggggt cccggcgcac gatctcgcga
     4861 tgatgatgac gatcgcgctg cggttcattc cgacgctgct cgaagaggcc gagaagatca
     4921 tgaaggcgca gatggcgcgc ggcgcggagt tcacccgggg cagctttccg gcgcgggccc
     4981 gggcgctcac ccccctgctc gtgccgctgt tcatcagcgc gttccggcgg gcggacgcgc
     5041 tggctgtggc catggaggcc cggtgttacc gcgggggcga gggccggacc cggatgaacg
     5101 tgctgtggtt ccgcccccgc gacgcgatcg ccttcacgct ggtcaccgcg gcagccgccc
     5161 tcgcgctgct ccccccggcc gcctggcggg ggcggtaggg catgcgcacc ctgaggctgg
     5221 tgctggagta cgatgggacc ggctactgcg ggtggcagcg gcagcgggag tccgcggccc
     5281 gggggcttcc gtccgtgcag gcgacgctcg aggcggccct gcgcgagatc acgggggagg
     5341 cgacccaggc cgtgggcgcg ggccgcaccg atgccggcgt ccacgcgctg gggcagatgg
     5401 cgcacttcac gaccgacagc cggatcccca ccagcgcctg gcccccggcg ttgcacgggc
     5461 atctcccccc cgacatccgc atcctcggcg cggacgaggt gccccccggc ttccacgccc
     5521 ggttcgatgc cgtctcccgg acctaccggt acgagatcct gaaccgcccg gcgccctcgg
     5581 cgctcctccg gcaccgggcc taccacgtcc cggagccgct cgacgtcggg gcgatgcagc
     5641 gggcgctggc ggcgctggtt ggccggcacg acttcgccgc ctaccggcgc atgggcagcc
     5701 cttcgcgaac gaccatctgc accgtcaccg cggccgggtg cgcccggacc ggctcgcggg
     5761 tgcgcgtgac gatcacggcg gaccggtttc tccggcacat ggttcggatg atcgtcggca
     5821 cgctggtgcg ggtcggcacg ggccggctcc ccgtagacgc gccgggcgcg tatttggccg
     5881 atcgggacag ccaaagaacc ggccccaccg ccccggccca cgggctgtat ctcgtccggg
     5941 tggagtacgg cccgcccgg
//
LOCUS       DASQGH010000548         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_55955, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000548 DASQGH010000000
VERSION     DASQGH010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55955"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1466
                     /locus_tag="VEW91_10815"
     CDS             135..1466
                     /locus_tag="VEW91_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYM92113.1"
                     /translation="MATVYGRQVVEQYLDHNRRSEELNKRARAVLPGGVTRTSVYFDP
                     FPPYIERGEGCRITDVDGNVRIDFSNNYTALILGHCPPPVTAAVQAQAARGSAFAAPG
                     RPEVTLAELITRRVPSVAQIRFASSGTEAVMFALRLARAFTGRSKIAKAEGGFHGTSE
                     YASISVSPDVAEAGPGPAPAALAASRGITRRVLDEVVVFPFNDAEATEAIIRRHRDDL
                     AAVIVEPMLGSSGMIPARREFLQRLRDLTARSGILLILDEIITLRLAPGGAQSLYGVS
                     PDLTVMGKIIGGGYPVAAFGGRGDIMALLDPEGGRPPVPQSGTYNGNPIGMVAGSVTL
                     GELTPAVYDRLNAMGEDLRARLGSLFRRKGVAAQATGMGSLLNLHFTEREVTDFRTMR
                     TGDAARMRQVFFGLLNEGIFLAPRGMACLSTPMGESEIDAFVRATERALEG"
     gene            1575..>2826
                     /locus_tag="VEW91_10820"
     CDS             1575..>2826
                     /locus_tag="VEW91_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYM92114.1"
                     /translation="MPDRITVSVLQHRFVAIVAEMGEAMLRTAYSQILNSSRDFSTAL
                     CDGQGRLVAQAEHVPIHVGALPWAVRSVAQAFEGRIAPGDLYLLNDPYHGGNHLPDLT
                     VLLPVFAEGRLVFWSINRAHQSDIGGSTHGAYNPGATEIWQEGIRIPPLKLYDAGEVR
                     DDVMQMIATNVRHARDFLGDLRASIGSARLGERGLLRLVDEYGVETVAAGAGEILDST
                     ERQTRACIRTWRDGAYRGEAVLDDDGHGATDIAIRATVTKTGDHLVVDLRESDPQVSG
                     FVNSSYPNTMSAVHMAIAYLIDPGIPKNDGTFRPVAVRARQGTIVWPFPPAPVTLATN
                     HCGQEIGEAVIKALARACPGRAVAGWSRRFRIAIRGVDPRTRRPFIWHMFHARGGGGA
                     AAAGDGWPTAGEGQAAGGIKFGSVE"
BASE COUNT          417 a          970 c         1022 g          417 t
ORIGIN      
        1 cccatcgccc attcccgcga tcggcgaacg ttgcagagtt gttaccggcg ggaaggggcg
       61 gcgggccgct caaaggatgc gttcgttgat ccacgaatta cctagcgatc caggcgatgg
      121 gaggcggtgg ggcgatggcg acggtgtacg gcaggcaggt tgtcgagcag tacctcgatc
      181 acaaccgtcg gtcagaggag ctcaataaac gcgccagggc ggtgctcccc ggcggggtca
      241 cgcgcacctc agtgtacttc gatccgtttc ctccgtacat cgagcggggc gagggatgcc
      301 ggatcaccga cgtcgacgga aacgtgcgga tcgacttctc gaacaactac accgcgctca
      361 tcctggggca ctgccctccc ccagtcaccg ccgccgtgca ggcgcaggcg gccaggggca
      421 gcgcgttcgc cgcgccgggc cggcccgagg tcacgctcgc cgagctgatc acccggcggg
      481 tcccctcggt cgcgcagatc cggttcgcga gctcggggac cgaggcggtg atgtttgccc
      541 tccggctggc cagggcgttc accgggcgct ccaagatcgc caaagccgag ggcgggttcc
      601 acggcacctc ggagtacgcg tcgatctcgg tcagccccga cgtggccgag gccggcccgg
      661 gccccgctcc cgcggcgctg gccgcgtcgc gggggatcac ccgccgcgtc ctggacgagg
      721 tggtggtctt ccccttcaac gatgcggaag ccaccgaggc catcatccgt cgccaccggg
      781 acgatctggc cgcggtcatc gtcgagccga tgttggggtc gagcggcatg atccctgcgc
      841 ggcgagaatt tctccagcgg ctgcgcgacc tcaccgcccg cagcggcatc ctgctgatcc
      901 tcgacgagat catcacgctg cgccttgccc ccggaggggc ccagagcctc tacggggtct
      961 cgccggacct cacggtcatg gggaagatca tcggcggcgg gtatccggtc gccgccttcg
     1021 ggggccgcgg ggacatcatg gcgctcctcg accccgaggg cgggcgcccg ccggtgcccc
     1081 agagcgggac ctataacggc aacccgatcg ggatggtggc cggaagtgtc accctgggcg
     1141 aactcacccc ggcggtgtac gaccgtctca acgcgatggg cgaggacctg cgcgcgcggc
     1201 tcgggtcgct gttccggcga aagggcgtcg ccgcccaggc gacggggatg gggtccctgc
     1261 tgaacctcca cttcaccgaa cgggaggtga cggatttccg gacgatgcgg acgggggatg
     1321 ccgccaggat gcggcaggtg tttttcgggc tgttgaacga ggggatcttc ctggcaccca
     1381 ggggcatggc gtgcctctcg accccgatgg gggagtccga gatcgacgca ttcgtgcggg
     1441 ccaccgagcg ggcgctcgag ggatgaccgc ggccatcctg gccgcgctcc gcgacgccac
     1501 cgggcgcgat ttgtaccgtg ccccggtccg caccgacgat ctggtcgggc agcaggagct
     1561 catcgggccc gagcatgcct gaccggatca ccgtctcggt cctgcagcac cggttcgtgg
     1621 cgatcgtcgc cgagatgggc gaggcgatgc tgcgcaccgc ctactcgcag atcctgaact
     1681 cgagccggga cttctccacc gcgctgtgcg atggccaggg ccggctcgtg gcgcaggcgg
     1741 agcacgtgcc gatccacgtg ggcgccctcc cgtgggccgt gcggtcggtc gcgcaggcgt
     1801 tcgagggccg gatcgccccc ggcgacctct acctgctcaa cgatccgtac cacgggggca
     1861 accacctgcc ggacctgacc gtgctcctcc ccgtttttgc cgagggccgc ctggtgttct
     1921 ggtcgatcaa ccgggcccac cagagcgaca tcgggggctc cacccacggg gcctataacc
     1981 ccggggcgac ggagatctgg caggaaggca tccgcatccc gccgctcaag ctctacgatg
     2041 ccggcgaggt gcgggacgac gtgatgcaga tgatcgcgac caacgtccgc cacgcccgcg
     2101 acttcctcgg ggatctccgg gcgtcgatcg gctcggcccg gctgggggag cggggtctgc
     2161 tgcgactggt ggacgagtac ggggtcgaga cggtggcggc cggggccggg gagatcctcg
     2221 acagcaccga gcgtcagacc cgcgcctgca tccgcacctg gcgggacggt gcgtaccggg
     2281 gcgaagcggt gctggacgac gatgggcacg gcgccaccga catcgcgatc cgggccaccg
     2341 tcacgaagac cggcgaccac ctcgtggtgg acctgcgcga gagcgatccg caggtgagtg
     2401 gattcgtcaa ctcctcgtac ccgaacacga tgtctgcggt gcacatggcg atcgcctacc
     2461 tgatcgaccc gggcatcccg aagaatgatg ggacgtttcg tccggtcgcg gtgcgcgccc
     2521 ggcaggggac gatcgtctgg ccgttcccgc ccgctcccgt gaccctcgcc accaaccact
     2581 gtggccagga gatcggcgaa gcggtgatca aggcgctggc ccgagcctgc cccggccgcg
     2641 cggtggccgg atggtcccgc cggttcagga tcgccatccg gggcgtcgac ccccggaccc
     2701 ggcgcccgtt catctggcac atgttccacg cgcggggcgg cgggggggcc gccgcggccg
     2761 gcgacggctg gcccacggcg ggtgaggggc aggcggccgg ggggatcaag tttggcagcg
     2821 tggagg
//
LOCUS       DASQGH010000549         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_27854, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000549 DASQGH010000000
VERSION     DASQGH010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27854"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VEW91_10825"
     CDS             complement(<1..580)
                     /locus_tag="VEW91_10825"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYM92115.1"
                     /translation="MPSARRIRPGFSLAWILVLTLALAPTAGAQPAPAGPPTFELPEV
                     EVAGKRPQPPSTTPASISVITADEIARLGALSVGDALRILAEVRIKDSGGPGSLTTVS
                     IRGSTSTQVLILLDGVPLNRPDQASVDLSTLPIQNVERIEVLRGPFSAIYGSPALGGV
                     INIVTRSAPQTLLSSRIGSCGLAGNVVSLGGQE"
     regulatory      complement(691..902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1153..2508
                     /locus_tag="VEW91_10830"
     CDS             1153..2508
                     /locus_tag="VEW91_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYM92116.1"
                     /translation="MHPILLDAAARANRYLEELETRSVAPSSEAVNRLPDLGGPLPQH
                     PTDPEGVLAALDAIGSPATVASAGGRYFGFVIGSSLPAALAANWLAGAWDQNAGPWVS
                     SPTSAVLEEIALRWLIDALQLPEQSGGAFVTGTTMANFSALAAARHAILARAGWDVEA
                     DGLFGAPPITVIVGDEVHVSLLKALSLLGLGRDRVVRIPTDDQGRMRAEALPPLTDRT
                     IVCAQAGNVNTGALDPVGEICGAAHDAAAWVHVDGAFGLWAAASPALAHLVRGAEDAD
                     SWAVDAHKWLNTPYDGGVVFVRDAGHLTAAMSASAAYLPQGGRRDPMRYTPEMSRRAR
                     GVEIWAALRSLGRSGLADLIERTCRYARRFSEGLASAGYPSLNEVVLNQVLVSFGDND
                     ITRLVIDGVQADGTCWCGGTVWRGRAAMRISVSSWATTQQDIDRSLAAVLRVAARSRR
                     P"
     gene            2752..>2888
                     /locus_tag="VEW91_10835"
     CDS             2752..>2888
                     /locus_tag="VEW91_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM92117.1"
                     /translation="MDGELSGQVVIITGAGRGIGRAAVGELAAAGARLVLVARTRADL
                     MR"
BASE COUNT          479 a          903 c         1017 g          489 t
ORIGIN      
        1 cttcctggcc gcccagcgag acgacgttgc cggccagccc gcacgatccg atccgggagg
       61 acagcaacgt ctgcggggcg gatcgcgtga cgatgttgat cacccctccg agcgccgggc
      121 tcccgtagat cgcggagaag ggcccccgga gcacctcgat ccgttccacg ttctggatcg
      181 gcagggtgct cagatccacg ctcgcctgat cgggccggtt gaggggcacc ccatcgagca
      241 ggatgagcac ctgggtggaa gtggagcccc ggatgctcac ggtcgtcagg gacccggggc
      301 ctccgctgtc tttgatccgg acctctgcca gaatgcgcag ggcatcgccc acgctcaacg
      361 cccccagcct cgcaatctcg tcggcggtga tcacgctgat cgaagccggt gtggtggacg
      421 ggggctgcgg ccgcttgccc gcgacctcca cctccgggag ctcgaaggtc ggcggcccag
      481 cgggcgcggg ttgcgccccc gcggtgggag cgagcgccag ggtcaacacc aggatccagg
      541 ccagggaaaa ccccgggcga atgcgccgtg ccgacggcat cgcgcgtccc tccgtctgcg
      601 gcgccgcgcc aaaaaaaccg gcccccatga gacccacggg gaccgagacg gcacgaagcc
      661 gcaagcgtac tatcctttca gccgaaggtc accgtacgca atcgggtggg caggtttcct
      721 ggctccggat cctcgccgct ccgcgccttc ccggttaccc agtggcgacc aattggagcg
      781 gctccccgtc acagtggcgg taccgcgccg gcattgcagc gctgcgggca cacccgcagg
      841 ccgcgcaccg gacttccctt ttactccccg ctacgaggag cacccatccg acacggtatg
      901 aagtttgatt cagagtatac gaccgcctcg cccggcggtc aagggaggtg tgagggtgct
      961 acttgtgggt cttcacgcga ggggtgctaa agtgagttct agacgttcgg tgaaattctt
     1021 tctagttgga tacggtccag tatcaacatc tagtgcctct ccaactcgat tgacaggtaa
     1081 agaaccaata cccggggaaa ggttgaactc atgaagcggg agggtgtgac accgactagg
     1141 gaggaggggc cgatgcaccc aattctgctt gatgcggccg ccagggcaaa tcgttacctg
     1201 gaggagctcg aaacgcgcag cgtcgcgccg tcctccgaag cggtaaaccg ccttcccgat
     1261 ctcggaggtc cgctccctca gcacccaacc gacccggaag gggttctcgc ggctctcgat
     1321 gccatcgggt cgccggcgac ggttgcatcg gccggcggac ggtatttcgg cttcgtcatc
     1381 gggagttcgc tccccgccgc gctggccgcg aactggctgg ctggggcgtg ggaccagaac
     1441 gccggaccct gggtgtcctc ccccacgagc gctgtgttgg aagagattgc cctccgatgg
     1501 ctcatcgacg cgctccagtt gccggaacag tctggaggcg cgttcgtcac cggcaccacg
     1561 atggccaact tctccgcact cgcagcggcc cggcacgcga ttcttgctcg cgctggatgg
     1621 gacgtggaag ctgatggcct gtttggagca ccgccgatca cggtgattgt cggcgatgag
     1681 gtgcacgtga gtctgctcaa agcgctcagc ctgctgggcc tcgggcgtga ccgtgtcgtg
     1741 cggatcccga cggatgatca gggacgaatg cgggcggagg cgctgccgcc gctgacggat
     1801 cgtaccatcg tctgcgcaca ggcgggtaat gtgaacaccg gcgcgctcga cccggtcggg
     1861 gagatctgcg gtgcggcgca tgacgcggct gcctgggtgc acgtcgacgg ggcgttcggg
     1921 ttgtgggcgg cggcctcccc cgccctcgcc catctggtcc ggggggcgga agacgcggat
     1981 tcgtgggcgg tggacgcgca caaatggctg aacacgccgt acgacggtgg ggtagtcttc
     2041 gttcgggacg cgggccattt gacagcggcg atgagcgcgt ctgctgcgta cctgccacag
     2101 ggcgggcggc gggacccgat gcggtacaca cccgaaatgt cgcggcgggc acggggggtc
     2161 gagatctggg ccgcgctccg cagcctcggc cggtcgggcc tcgccgatct catcgagcgc
     2221 acgtgccggt acgccaggcg tttctccgaa ggactcgcga gcgcagggta tccgtccctg
     2281 aatgaggtgg tgctcaatca ggtgctcgtg tcgtttggcg acaacgacat cacgcgcctg
     2341 gtaatagacg gtgtccaagc cgatggaacc tgttggtgcg gaggaacggt gtggcgtggc
     2401 cgggccgcca tgcgaatcag cgtctcgtca tgggccacga cgcaacagga tattgaccgg
     2461 tcgctggctg ccgtgctgcg ggttgccgcg agaagcagac ggccttgatg ggagtggggc
     2521 tggtcgaact agcggtagat ctcgttgtga cagacgctta gacggcccgg ctcgggaaaa
     2581 aatgcgattc catggggatt ggtggctcca tcggttcaac gcctcggggc ctgcggggca
     2641 gggtctgggc cgaaaaccgg gctgaaacct tccaatgggg catggcgtaa tcgccatgca
     2701 aagagcacca cctggccgct gtaccgaatc cgatccatcg gaggtgcatg gatggacggg
     2761 gagctttctg ggcaggtcgt catcatcacc ggcgcggggc gcgggatcgg gcgtgcggcg
     2821 gttggggagc tggccgcggc gggcgcgcgg ctcgtgctgg tcgcgcggac gagagccgat
     2881 ctcatgcg
//
LOCUS       DASQGH010000550         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44871, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000550 DASQGH010000000
VERSION     DASQGH010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44871"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VEW91_10840"
     CDS             <1..355
                     /locus_tag="VEW91_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HYM92118.1"
                     /translation="YSKLAAYKKRMGWSFKWVSSSETDFNFDYHVSFTPEELAKNEAF
                     YNYTIQNPYESEREGVSAFFKDPAGSVFHTYSTYARGIDMLNVDYHYLDLVPKGRDEA
                     GEGPFWVRRHDEYGR"
     gene            426..815
                     /locus_tag="VEW91_10845"
     CDS             426..815
                     /locus_tag="VEW91_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYM92119.1"
                     /translation="MSKTIRQSVTIKASPHAVYEALMDSRKHAAFSGMPARISRKVGG
                     PFTAYGPYLSGVNLELVPDRRIVQFWRSRNWPRHHYSTVTFALTKVPGGTRLRFTQAG
                     VPNGDYKDKKSGWITHYWKPMKAMLER"
     gene            complement(829..1548)
                     /locus_tag="VEW91_10850"
     CDS             complement(829..1548)
                     /locus_tag="VEW91_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYM92120.1"
                     /translation="MPGDLRTLLAAAAGVLGGVAVYTVGTQMVAGAIRGGVIKRGPAR
                     PMVALTFDDGPDPEHTPHILEALANAGVQATFFMIGRHAEAAPEVARAAAAAGHEVGN
                     HTYRHRHLWTLPPRATVAEVDRGAAAVADATGVFPRYFRPPWGMFNWAAYVRAGQLGE
                     TRILWSVRPEGWLSPVTAGEMAARVIRWTHPGAIIDLHDRGGHPTTPRATWAALPGMV
                     AGLRALGYRVVPLGTLLDPAS"
     gene            complement(1557..2717)
                     /locus_tag="VEW91_10855"
     CDS             complement(1557..2717)
                     /locus_tag="VEW91_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92121.1"
                     /translation="MKSPSETALLATTVGLVEFVHGALLIVLLPTLVAQRLGWAMGTT
                     GAAVSAYFFTEVAAKLVSGWLVDRVGARRMLLWGFWSYAAGFAALASSRTTWQVFLAL
                     SAMGAGASPLWPAALTRLTRSAGPQVGEALGNVFSTWFLGAGLGIGVATLVSRTNHPI
                     SLSVFILPLLGAALLGLLVPGDPANPTPVGGRPPVGQLLRLVGASLVKLATSIIPQII
                     AAGVLIPVVVPYLETVRGLDERQLLVLMVLGIGTGLLLLTPAGRIGDRVGRRRTYALG
                     LGVVAVLLLAVPFCHSLWLLALDFIGLGVGYAFVLPAWNSILLRLLPVDIRGSGLGVL
                     MTVEGMGGVIGPLVGGLLWQWSHPSAPFYLSGGLLLLASGASVRWRSDVVDC"
     gene            complement(2714..>3149)
                     /locus_tag="VEW91_10860"
     CDS             complement(2714..>3149)
                     /locus_tag="VEW91_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYM92122.1"
                     /translation="QVRARTARAPHPFHAFGYVDNVEELMAVSDLLITKAGGVTVSEA
                     LAMGLPMLIYRPIPGQEEGNTAYLLEHGTALAPRTPAMLHTSLETLLSDPARIEAMRR
                     AAKQLARPDATDQVIAHLAALAARADAPSTGQRTLAISTPRT"
BASE COUNT          550 a         1062 c         1106 g          431 t
ORIGIN      
        1 gtacagcaag ctggcggcat acaagaagcg gatggggtgg agcttcaagt gggtctcctc
       61 gtcggagacc gatttcaatt tcgactacca cgtgtcgttc acgccggaag agctggccaa
      121 gaatgaagcc ttctataact acacaataca aaacccatac gaatcagagc gggaaggcgt
      181 cagcgcattc ttcaaggacc cggcgggaag cgtgtttcac acctattcaa cctacgcgcg
      241 tggcatcgac atgttgaacg tggactacca ttacctggac ctcgtgccca agggccgaga
      301 tgaggctggt gaggggccct tctgggtgcg ccgccacgat gaatacgggc gttagttcgc
      361 aaacacacgc tgagcgtttc attggcgatt gctatgtttt gaaccaacgg aggtgaagcg
      421 gagcaatgtc caagacgatc cggcagtcgg tgacgatcaa ggccagtccc cacgcggtgt
      481 acgaagcgct catggattcg cgcaagcacg ctgcgttcag cgggatgccc gcgcgcatca
      541 gccgcaaggt gggaggcccg tttacggcct acggccccta cctgagcggc gtgaacctcg
      601 aactggtccc ggaccggagg atcgtgcagt tctggcggtc ccggaactgg cccaggcacc
      661 actattccac ggtcacgttt gccctgacga aggtcccggg cgggacccgc ctgcgattca
      721 cccaggcggg cgtgccgaac ggcgactaca aggataagaa atcggggtgg atcacccact
      781 actggaagcc gatgaaagcc atgctggaac ggtaagtccg cgggacgcct acgacgccgg
      841 gtcgagcagc gtccctagag ggacgacccg gtacccgagg gcccggagcc cggcgaccat
      901 gcccgggagg gcggcccacg tggcccgggg cgtcgtgggg tgccctccgc ggtcgtgcag
      961 gtcgataatg gccccagggt gcgtccagcg gatcacgcgc gcggccatct cgcccgcggt
     1021 gaccgggctg agccaccctt cgggccgcac cgaccacagg atccgcgtct cccccagctg
     1081 ccctgcccgg acgtacgcgg cccagttgaa catcccccag ggcggccgga agtagcgggg
     1141 aaagaccccg gtcgcatcgg ccaccgcggc ggccccgcga tccacctcgg cgaccgtggc
     1201 ccggggaggc agggtccaga gatgccggtg ccggtaggtg tggttgccga cctcgtggcc
     1261 ggcggccgcc gcggcgcgtg ccacctcggg agcggcttcg gcgtggcgcc cgatcatgaa
     1321 gaacgtggcc tgcaccccgg cgttggcgag cgcctcgagg atgtggggcg tgtgctccgg
     1381 gtccggcccg tcgtcgaacg tcagcgccac catcggacgc gccggcccgc gcttgatgac
     1441 gccaccccgg atcgcgccgg cgaccatctg cgtgccgacc gtgtacaccg cgacgccccc
     1501 gagcaccccg gcggctgccg caagaagcgt ccggagatcg ccgggcacgc tccgcgctag
     1561 cagtcgacca cgtccgagcg ccaccgcacc gatgcgccgg aggccagcag cagcaggccc
     1621 ccgctcaggt agaatggcgc ggagggatgc gaccactgcc acaggagccc gccgaccagc
     1681 gggccgatca cgccccccat gccttcgacc gtcatgagga ctcccaaccc ggagccccgg
     1741 atgtccaccg ggagcaggcg aaggaggatg ctgttccacg ccgggaggac gaacgcgtag
     1801 ccgaccccca gcccgatgaa gtccagcgcc agcagccaca gcgagtggca gaacggcacc
     1861 gcgagcaaca gcaccgccac gacgcctaag ccgagcgcgt aggtccgccg gcgtcccacc
     1921 cggtcgccga tccgtcccgc gggggtgagg agcagcaatc cggtgcctat gccgagcacc
     1981 atgagcacca gcagttggcg ttcgtcgagc ccgcgcaccg tctcgaggta ggggaccacg
     2041 accgggatca gcacgcccgc cgcgatgatc tgggggatga tgctcgtcgc gagcttcacg
     2101 agcgaagcgc cgacgaggcg gagcagctgg ccgacgggcg gtcggcctcc gaccggggtc
     2161 gggttcgccg ggtcccccgg cacgagcagg ccgagcagcg ccgctcccaa gagcggaagg
     2221 atgaacacgc tgagcgagat ggggtgattg gtccggctca cgagtgtcgc caccccgatg
     2281 ccgagccccg cgccgaggaa ccaggtggaa aacacgttcc cgagcgcctc cccgacctgg
     2341 ggtccggcgc tccgcgtcag gcgggtgagc gcggcgggcc acagcggcga ggcgccggcg
     2401 cccatggccg agagcgcgag gaatacctgc caagtggtcc gcgagctcgc gagcgcggcg
     2461 aagcccgcgg cgtagctcca gaatccccac agcagcatcc gcctggcgcc gacacggtcg
     2521 accagccatc cggagaccag tttcgcggcc acctcggtga aaaagtaggc ggagaccgcg
     2581 gcgccggtcg tgcccatcgc ccatccgagc cgctgcgcta cgagcgtcgg caggaggaca
     2641 atgagcaaag cgccgtggac gaactcgacc aggccgaccg tggttgcgag gagcgcggtt
     2701 tcgctcgggg atttcaagtc cgaggggtgg agatcgctag cgtcctttgt cccgtgctcg
     2761 gggcgtcggc ccgggccgcg agggcggcca ggtgggcaat cacctgatcg gtcgcatcgg
     2821 gtcgggcgag ctgcttggcg gcccgccgca tcgcctcgat gcgcgcgggg tcgctgagca
     2881 gcgtttcgag tgacgtgtgg agcatcgccg gggtcctggg ggcgagcgcc gtgccgtgct
     2941 cgaggaggta ggcggtgttc ccttcctcct gcccggggat cggccggtag atgagcatcg
     3001 ggaggcccat ggcgagcgcc tcactcaccg tgacgcctcc ggccttggtg atgagcaggt
     3061 cgctcaccgc catcagttcc tcgacgttgt ccacgtagcc gaaggcgtgg aaggggtgcg
     3121 gggccctggc ggtgcgcgcg cgcacctgc
//
LOCUS       DASQGH010000551         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50548, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000551 DASQGH010000000
VERSION     DASQGH010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50548"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VEW91_10865"
     CDS             <1..300
                     /locus_tag="VEW91_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM92123.1"
                     /translation="ARRGGGPGFLLCHTYRFRGHHVGDINRASYRSTDEEETWTRERD
                     PIRLLGLFLIQQRLADAAGLERIVQGVRAEIAAGVQFALEAPYPDPGEVTQNVYP"
     gene            334..1314
                     /locus_tag="VEW91_10870"
     CDS             334..1314
                     /locus_tag="VEW91_10870"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYM92124.1"
                     /translation="MRELTFGQAIKEALAEEMRRDPSVFVIGEDVAEAGTVFRVLAGL
                     VDEFGRERVIDSPISEAGITGLGVGAAMTGMRPVVDIMFGDFITLAMDQIVNQAAKVH
                     YMSGGKLRVPLVIRTTMGATRRSAAQHSQSLYAWIAHVPGLKVVLPSTPADAKGLLKS
                     AIRDDSPVVFFEDKMMYQLKGPVPEGEYTVPLGVADVKRAGADVTIVATSSMVHVALD
                     AAARLEGEGIGAEVVDPRTLLPLDAATLVASAKKTGRVIVVDEGYGQYGVTGEIASVI
                     TEGAFDHLDAPVRRMGAMNVPIPFSPVLEDQTVPTATAVADAARHLCGRA"
     gene            1327..2697
                     /locus_tag="VEW91_10875"
     CDS             1327..2697
                     /locus_tag="VEW91_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM92125.1"
                     /translation="MGLKTYGLMGVDWEERVNYDRLRKERLERAQRELGRSEVGALLC
                     FDMNNIRYLTATHIGTWAMDKLVRFSLLPRGHEPIVWDFGSAARHHQLYCPWLDGRSP
                     AGISTLRGAMSPESGRAEDVAKKIRRELEARGLHRDPIGVDVIELPILFALQAAGLKV
                     VDGQQVMQRARLIKTQDEITLLNTACMMVDAAYDKLYRAMKPGMRENECVGLVAKVLY
                     DLGSEHVEGVNAISGERCSPHPHVYTDRALRPGDPAYFDILHSYNGYRTCYYRTFAIG
                     SASPAMVDAYERCREYLDAAIALIKPGVTTGDVVKVWPKAEEFGFPNEEAAFALQFGH
                     GVGLSIWEKPIFSRLVSLDHPETIQEGMVFALETFWPARDGWSAARIEEQLVVTKGGC
                     EVITRCPAEELLVAGAHHYTVAGPLPTTRETQSHLNRRARTRTNAGTLGEVVAGAKTE
                     GRRVRT"
     gene            complement(2708..3994)
                     /locus_tag="VEW91_10880"
     CDS             complement(2708..3994)
                     /locus_tag="VEW91_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92126.1"
                     /translation="MLQDAGEGFSWSVGDPCPPPIEGDLVPTWQRNIYAIWSAQFLAM
                     IGLTLIAPFLPFYLRLLGITTVEDVERWSGLVFAAPFAVQTLAAPLWGVAGDRYGRKI
                     MVLRAMVGIGTTSMLSATVRYVGQLIFLRAVQGGVSGFVAASNALVTASIPPDRMGTA
                     LGVLQTSLTAGGIIGPLIGGALADLVGYRNVFLITGAMCYAAAVVVLRGVHEEARPAH
                     ARSGGPGVLANLGTFLGSPALRTTGLLLCTTQIAIMSVEPIFAVYVSMLRVPPDRVAT
                     VAGVLFAVAGFTSMLGAPLWGRIADRAGGQRVLALTLGGAAVAYAAQAGAGSPWDLFV
                     FRAAAGFFTGGMLPPLYAIVARSAPPERLGGIMGLTSSAIMLGSVIGPLVGGLLSAAV
                     GIRWVFLVAAAVLGISALGVRGLGPGAQAKIENPVR"
     gene            4060..>4656
                     /locus_tag="VEW91_10885"
     CDS             4060..>4656
                     /locus_tag="VEW91_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYM92127.1"
                     /translation="MQAKTVLFEMLRDRGVRHVFGNPGTTELNFMEMFADYPEITYVL
                     ALQDAIPVGMAYGYAQATGAPAFVNLHITPGLANGLGNIFNAYKARTPMVITAGQVDR
                     RMILQEPTLWSDLARLASQYTKWAYEVRTGADIPVALARAFKTAAAPPPGPVFLSLPM
                     DCLDEEAAGPAPWIDVAGGARPAPAVVERIAGELAGARG"
BASE COUNT          764 a         1608 c         1660 g          624 t
ORIGIN      
        1 gcccgccggg gcggcggacc gggcttcctg ctgtgccaca cctaccggtt ccgcggacac
       61 catgtcggcg acatcaaccg ggcctcctac cgatccaccg acgaagaaga aacgtggacc
      121 cgcgagcggg acccgatccg cctcctcggt ctcttcctca ttcagcagcg gctggcggac
      181 gccgcggggc tcgagcggat cgtgcagggc gtccgcgccg agatcgccgc cggggtgcag
      241 tttgccctcg aggcccccta cccggacccc ggcgaggtga cccagaatgt ctatccctga
      301 tcagacaatc gtcgcggggg tgagagggtg gcggtgcgcg aactgacctt tgggcaggcg
      361 atcaaggaag cgctggcgga ggagatgcgc cgcgacccga gcgtgttcgt catcggggag
      421 gacgtggccg aggcgggcac ggtgttcagg gtcctcgccg ggctcgtcga cgagtttggg
      481 cgcgaacgcg tcatcgactc gcccatctcc gaagccggca tcaccggcct gggggtcggg
      541 gccgccatga ccgggatgcg cccggtcgtg gatatcatgt tcggcgactt catcacgctc
      601 gccatggacc agatcgtgaa tcaggcggcc aaggtgcact atatgtcggg cggcaagctc
      661 cgggtgccgc tcgtgatccg gacgacgatg ggcgcgaccc ggcggtcggc ggcgcagcac
      721 agccagagcc tgtacgcgtg gatcgcccac gtccccgggc tgaaggtggt cctcccgtcg
      781 accccggccg acgccaaggg gctgctcaag tcggcgatcc gcgacgacag cccggtcgtt
      841 ttcttcgagg acaagatgat gtaccagctc aaggggcccg tgccggaggg ggaatacacc
      901 gtgcccctcg gggtcgcgga cgtcaagcgc gcgggcgccg acgtcaccat cgtcgccacc
      961 agcagcatgg tgcacgtggc gctggatgcg gccgcgcggt tggagggcga ggggatcggc
     1021 gccgaggtgg tcgacccgcg cacgttgctg ccgctggacg ccgccacgct ggtcgcgtcg
     1081 gccaagaaga ccggccgggt gatcgtggtg gacgaagggt acgggcagta cggggtgacc
     1141 ggagagatcg cgtcggtgat caccgagggg gccttcgacc acctggacgc cccggtccgg
     1201 cgcatgggcg cgatgaacgt gccgatcccg ttctccccgg tcctggaaga ccagaccgtc
     1261 cccaccgcga cggcggtggc ggacgcagcc aggcatctgt gcgggcgcgc gtaaggaggc
     1321 acaccgatgg ggttgaagac gtacgggctc atgggggtcg actgggagga gcgggtcaac
     1381 tatgaccggc tgcggaagga gcgcctggag cgggcccagc gcgagctcgg gcggtcggag
     1441 gtgggcgcgc tcctgtgctt cgacatgaac aacatccggt atctcacggc cacccacatc
     1501 gggacgtggg cgatggacaa actcgtgcgc ttctcgctgc tgccgcgggg ccacgagccg
     1561 atcgtgtggg atttcggctc agcggcgcgc caccatcagc tgtactgccc ctggctggac
     1621 ggccggtcgc cggccgggat ctccaccctg cggggggcga tgtcccccga atccgggcgg
     1681 gcggaggacg tggcgaagaa gatccggcgg gagctcgagg cgcgcggcct ccaccgggac
     1741 ccgatcgggg tcgacgtcat cgaactgccg atcctgttcg cgctgcaggc cgcggggctc
     1801 aaggtggtcg acgggcagca ggtgatgcag cgcgctcgcc tcatcaagac ccaggacgag
     1861 atcaccctcc tcaacaccgc gtgcatgatg gtcgatgcgg cctacgacaa gctctaccga
     1921 gcgatgaaac ccggcatgcg ggagaacgaa tgcgtcggat tggtcgccaa ggtcctctac
     1981 gacctcggct cggagcacgt cgaaggcgtc aacgcgatct ccggcgagcg gtgcagcccc
     2041 cacccgcacg tctacaccga ccgcgccctc cgcccggggg atccggcgta cttcgatatc
     2101 ctccacagct acaacggcta ccgcacgtgc tactaccgca ccttcgccat cgggagcgcc
     2161 tcgcccgcga tggtcgacgc ctacgagcgc tgccgcgagt acctggacgc ggcgatcgcc
     2221 ctgatcaagc cgggggtcac cacgggggat gtggtcaagg tctggccgaa ggcagaggag
     2281 ttcgggttcc ccaacgagga ggcggcgttc gcgctgcaat tcgggcacgg ggtcggcctc
     2341 tcgatttggg aaaagcccat cttcagccgc ctggtctccc tcgatcaccc ggagacgatc
     2401 caggaaggca tggtcttcgc cctcgagaca ttctggccgg cgagagacgg ctggtcggcg
     2461 gcgcggatcg aggagcagct ggtggtgacg aagggcggct gcgaggtcat cacccggtgc
     2521 ccggccgagg agctgctggt tgccggcgcg caccactaca cggtggcagg accccttccc
     2581 acgacccggg agacccagtc ccacctgaac cggcgcgccc gcacgcggac gaacgcgggc
     2641 acgctcggcg aggtggtggc cggcgcgaag accgagggcc gcagggtgcg gacgtaggcg
     2701 ggccggctca tctgacgggg ttctcgatct tcgcctgagc ccccgggccc aggccccgga
     2761 ccccgagggc cgagatcccg agcaccgccg cggcgacgag gaacacccag cggatcccga
     2821 cggcggcgga caggagaccg ccgacgagcg ggccgatcac gctgcccagc atgatcgcgc
     2881 tgctcgtgag tcccatgatc cccccgagcc gctccggggg tgccgagcgc gccacgatcg
     2941 cgtacagggg cggcagcatg ccgccggtga agaaccccgc ggcggcgcgg aacacgaaga
     3001 ggtcccacgg agaccccgcc cccgcctgcg ccgcgtacgc caccgccgcc ccccccaggg
     3061 tcagcgcgag cacccgctgt ccgccggccc ggtcggcgat ccgcccccac agcggcgcgc
     3121 cgagcatcga ggtgaatccg gcgaccgcga agaggacgcc ggccacggtc gcgacgcggt
     3181 cgggggggac ccgcagcatg ctcacgtaga cggcgaagat gggctcaacg ctcatgatgg
     3241 cgatctgggt cgtgcagagc agcaacccgg tggtccggag ggccggggac ccgaggaagg
     3301 tgccgaggtt ggccaagacc cccgggccgc cgctcctcgc atgcgctggc cgggcctcct
     3361 cgtgcacccc gcggaggacg accaccgcgg cggcatagca catcgccccg gtgatgagga
     3421 agacgttgcg gtatccgacg agatcggcca gcgctccgcc gatgagcggg ccgatgatgc
     3481 ccccggcggt cagcgaggtc tgcagcaccc ccagggcggt ccccatccgg tccggaggaa
     3541 tcgacgcggt gacgagcgcg ttgctggccg ccacaaagcc gctcaccccg ccctggacgg
     3601 cgcggaggaa gatcagctgc cccacgtacc ggacggtggc ggagagcatg ctggtcgtcc
     3661 cgatgcccac catcgcgcgg aggaccatga ttttccggcc gtagcggtcg cccgccacgc
     3721 cccacagcgg cgccgccagc gtctggaccg caaagggcgc agcgaacacc agcccgctcc
     3781 accgctccac gtcctcgacg gtcgtgatgc ccaggaggcg gaggtagaag ggcaggaacg
     3841 gcgcgatcaa ggtgagcccg atcattgcga ggaactgcgc ggaccagatc gcgtagatgt
     3901 tccgctgcca cgtgggcacg agatccccct cgatcggcgg tgggcagggg tcgcccacgc
     3961 tccacgagaa tccctcgcct gcatcttgca gcaccgcagt ggaggcgccc ctgtgcttcg
     4021 atggcagcgc cgacacttcc cgtgcccagg ggggaaccga tgcaggccaa gaccgtcctc
     4081 ttcgagatgc tccgcgaccg cggcgtccgg cacgtgttcg ggaaccccgg gacgaccgag
     4141 ctcaacttca tggagatgtt cgccgactac cccgagatca cgtacgtgct ggccctccag
     4201 gacgccatcc ccgtcgggat ggcctacggg tacgcgcagg ccaccggggc ccccgcgttc
     4261 gtgaatctgc acatcacccc cggcctcgcg aacgggctgg ggaacatctt caacgcctac
     4321 aaagcgcgca cgccgatggt catcaccgcc ggccaggtgg atcgccggat gatcctccag
     4381 gaaccgacgc tgtggagcga cctcgcccgc ctcgcctcgc aatatacgaa gtgggcctat
     4441 gaggtgcgga ccggagcgga tatcccggtg gcgctcgcga gggcgttcaa gaccgccgcc
     4501 gctcccccgc cgggcccggt gttcctgtcc ttgccgatgg actgcctcga cgaagaggcg
     4561 gccgggccag cgccgtggat cgacgttgcg ggcggagcgc ggccggcccc ggccgtcgtg
     4621 gagcgaatcg ccggagagct ggccggagcc cggggc
//
LOCUS       DASQGH010000552         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30856, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000552 DASQGH010000000
VERSION     DASQGH010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30856"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEW91_10890"
     CDS             <1..213
                     /locus_tag="VEW91_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYM92128.1"
                     /translation="VRTLGSGDYFGELALVREIPRTATVVAKEPTTCLALTRWDFKGI
                     LDANPQIAIRLLETVAGRIQEDEAGR"
     gene            complement(252..671)
                     /locus_tag="VEW91_10895"
     CDS             complement(252..671)
                     /locus_tag="VEW91_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYM92129.1"
                     /translation="MFRRNSKIALLEEVPLFRGLARKHLEQVGRLADEIEVRTGRRLV
                     TAGERGHQLFVIVEGRAVARVGRGRTVRLGPGEFFGEMSLVDGGPCAATVEAATDMRL
                     LVVGHREFWALLNAAPPLVAKIMATLSRRVRDAETSV"
     gene            complement(754..1977)
                     /locus_tag="VEW91_10900"
     CDS             complement(754..1977)
                     /locus_tag="VEW91_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HYM92130.1"
                     /translation="MSEISDVVVVGAGPGGSAAAYYLARRGLDVLLLDKSEFPRDKIC
                     GDGLTPRAVGVLDDMGVLDSLLAVGQRIGGMEIFAPDGYPTAAPLPPWNGLPASMLVV
                     PRVALDDAIRKRALGSGARFAGQVIVHGVEAIGNGVVVTGERGRLPVTFRARIAILAT
                     GASVSLLVRLGLLPQAPPMMLAARAYFEGMRDLADRIQIRLDGVPLPGYGWIFPLSSA
                     SANIGAGFFPRARGGHRLSVTPRAAFDSFTRGRRVGAMLARARQCGPVRSYPIRVDFP
                     AFPTSGDRILLVGEAAGLVNPLTGEGIDYALESAQIAAQHVARMFDTGDLSRRSIAGY
                     DRLLRDRFERLFVFCRRVRDLALYPVILNRLARVAARREDLKMALTNIVLGNRPASTA
                     FSPTAMLRQVIPLGR"
     gene            2288..3145
                     /locus_tag="VEW91_10905"
     CDS             2288..3145
                     /locus_tag="VEW91_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HYM92131.1"
                     /translation="MEQPQRRGGEDHLGRRWLGILVLAIVAGGIAFGIAATRVWWLLP
                     LASAQGALVDRIFYTILAVTATAFVVMHVWLALSLMRSRRRGEVGAIAAPGPFRPAWM
                     AGPALVVGGGVLLLCVLEVVLLTLSEADWAKIYSATPDAAAPVEIVGRQFFWYVRYPG
                     PDGRLGRTNPRWVSPDNPLGIDPRDPAGKDNIVVVNELHLVVDRPVRVTVGSLDVIHS
                     FFLPNFRVKQDAVPGHPVEIWFTPNREGTYQIECSQLCGVGHYTMRGNVTVESQAAFA
                     RWMRTQGRP"
     gene            3184..>4122
                     /locus_tag="VEW91_10910"
     CDS             3184..>4122
                     /locus_tag="VEW91_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYM92132.1"
                     /translation="MIGHRESFVRHYIFSQDHKVIGIQYLFAGMFMALVGAGLAMLMR
                     LHLGWPDRGALNPQEYLGAVTMHGTIMVLFFLTPILASAFGNFLIPLMIGARDMAFPA
                     LNMLSFWTLVPGLLILLVSFFVPGGPAGNGWTAYPPLSAVPAAAPGSGLGQSLWLLAV
                     VSLCLSSTFGAINFITTVFNLRTNGMTPGRLPMTVWGILVTAVLALLTFPVLLAAGLM
                     LLLDRVAGTAFFVPLVVIGGQIRGQAGGSPLLWQHLFWFFGHPEVYIIILPAMGIISD
                     VLATFARTPLSGYRAIVLSLAAIGFISFLVWGHHMYV"
BASE COUNT          644 a         1415 c         1369 g          694 t
ORIGIN      
        1 gtgcggaccc tgggctcggg cgattacttc ggggaacttg cgctcgtgcg ggagatcccc
       61 cgcaccgcca cggtcgtcgc gaaggaaccc acgacctgcc tggcgctgac gcgctgggac
      121 ttcaagggga tcctcgacgc caacccgcag attgcgatcc ggctgctcga aacggtggcg
      181 gggcgcattc aggaggacga ggccggccgc taaccggtcc gagccagccc ggcagatccg
      241 cgacgccggc tctacaccga ggtctccgca tcgcgcacgc ggcgcgagag cgtggccatg
      301 atctttgcca cgagaggcgg tgccgcgttc aggagcgccc agaactcgcg gtggccgacc
      361 accagcagcc gcatgtccgt cgctgcctcg accgtggccg cgcagggtcc gccatcgacc
      421 aggctcatct cgccgaaaaa ctcccccggt ccaaggcgca ccgtccgacc ccggccgacc
      481 cgtgccaccg cccgcccctc gacaatgacg aacaactggt ggcccctttc cccggcggtc
      541 accagccgcc ttccggttcg gacttcgatc tcatcggcga gccgtccgac ctgttcgaga
      601 tgcttgcgcg ccagaccccg gaacaagggc acctcttcga gcagcgcgat cttgctgttc
      661 ctcctgaaca tcgggcacgg ccccccctcg aaagattggt gctctctacg atgctgaagg
      721 gtgcgtctcc ttcggcacgg gacgctgggg tggttaccgg cccaggggga tgacctgcct
      781 gagcattgcc gtgggggaaa aggccgtgga ggccggccga ttgcccagca caatattggt
      841 gagggccatc ttgaggtcct cacggcgcgc ggccacgcgt gccagccggt tgagtatcac
      901 gggatagagg gcaaggtccc gcacccgccg gcagaaaaca aacaatcgct caaaacggtc
      961 gcgcagcaac cggtcgtagc ctgcgatgct cctccgcgac agatccccgg tgtcgaacat
     1021 gcgggccacg tgctgggctg cgatctgggc agactcgagc gcgtagtcga tcccctcccc
     1081 ggtcaacggg ttgaccagcc cggccgcctc gcccaccagt aggattcggt cgccggaggt
     1141 tgggaacgca ggaaaatcca ccctgatggg gtagctcctc acggggccgc actgccgggc
     1201 ccgcgccagc atcgcgccca ccctccggcc ccgggtgaag ctgtcgaacg ccgcccgggg
     1261 agtcacggag aggcggtgtc ccccccgggc acgggggaag aaccctgccc cgatgttggc
     1321 cgaggcgcta gagagcggga agatccagcc gtagcccggc aggggcacac cgtccaagcg
     1381 gatctgaata cggtcggcca gatcacgcat cccctcgaag tacgcacgcg cagccagcat
     1441 catcggcggc gcctgtggca ggaggcccag gcgaaccagg aggctcacgg aggcgcctgt
     1501 tgccaggatg gcgatgcggg ccctgaacgt gaccgggagg cgaccgcgct cgcccgtcac
     1561 aacgacgccg ttgccgatcg cctcgacgcc gtgcacgatg acctggccgg cgaaccgggc
     1621 cccgctcccc agcgcgcgct tccggatcgc gtcgtccagc gcgacccgcg ggacaaccag
     1681 catggacgcc ggcagcccgt tccacggcgg gagcggcgcc gcggtcggat acccgtcggg
     1741 ggcgaagatc tccatgccgc cgatgcgctg ccccaccgca agcaggctgt ccagcacacc
     1801 catatcgtcg agcacgccga cggccctggg ggtcaggccg tctccacaga tcttgtcgcg
     1861 agggaactcg gatttgtcga ggaggaggac atcgaggccg cgccgggcca gatagtatgc
     1921 ggctgcggat ccgcccgggc cggcgcccac cacgactacg tcgctaatct cactcatgag
     1981 cgccggtgtc ttcgccgtgg ggtgcgggtg accctatcga acggcgtgat cgggcgactt
     2041 ggctcctcgg gtaggattcg aacctacaac cctccggtta actttacaca agcccggacc
     2101 catcgtagta tacgtaccgt gcacccaagc ggggtttctc gcgctgtcaa cagaaccctg
     2161 agtcgcgaag cggcctgttg cgtttgtatc agccgggcgc ctcggctgtg aggcgcatgc
     2221 cgtagagggg cgcaccatcg cgcgtgaccg cagaaggcga tcgcgggggg gcctggaggg
     2281 cgaccagatg gagcaaccac agcgccgcgg cggagaggac cacctgggca ggaggtggtt
     2341 ggggatcctt gtgctggcca ttgtcgccgg agggatcgcc ttcggcatcg ccgccacacg
     2401 cgtctggtgg ctcctcccgc tggcctccgc ccagggggcc ctcgtcgacc ggatcttcta
     2461 cacgatcctc gcggtcacgg ccaccgcgtt cgttgtcatg cacgtgtggc tagcgctgtc
     2521 cctgatgcgg tcccgccgcc ggggcgaagt cggagcgatt gccgcacccg ggccgttccg
     2581 gcccgcgtgg atggcgggac cggcgctggt cgtcggcgga ggtgtcctgc tgctctgcgt
     2641 gctcgaggtc gtcctcctca ccctgagcga ggcggactgg gcgaagattt acagcgcgac
     2701 cccggatgcc gccgcgccgg tggagatcgt cggccggcag ttcttctggt acgtccgcta
     2761 cccgggaccg gacgggcggc ttggccggac caatccgaga tgggtgtccc ccgacaatcc
     2821 cttggggatc gacccgcggg atccggccgg gaaggacaac atcgtcgtgg tgaacgagct
     2881 gcatctggtg gtggacaggc cggtccgggt gaccgtggga tcgttggacg tcatccacag
     2941 tttcttcctc ccgaacttcc gcgtgaagca ggatgccgtg ccgggccacc cggtggagat
     3001 ctggttcacg cccaatcgcg aaggcacgta tcagatcgaa tgctcgcagt tgtgcggggt
     3061 gggacactac accatgcggg gaaacgtgac cgtcgagtcg caggcggcgt tcgcccggtg
     3121 gatgcggact cagggacggc cgtaggtctc gagcctgcga cgaggaggtc gggcggtgac
     3181 gtcgtgatcg ggcacagaga aagcttcgtg cgccactaca tcttcagcca ggaccacaaa
     3241 gtcatcggca tacagtatct attcgcgggg atgttcatgg ccctggtcgg cgccggcctc
     3301 gccatgctga tgcggctgca cctcggctgg ccggaccgcg gcgccctgaa cccgcaggag
     3361 tacctgggcg ccgtcaccat gcacggcacg atcatggtcc tcttcttcct cacgccgatc
     3421 ctggcgagcg cgttcgggaa ttttctgatc cccttgatga tcggcgcgcg ggacatggcg
     3481 ttccccgcgc tcaatatgct ctcgttctgg acgctcgtgc caggcctgct catactgctc
     3541 gtctcgttct tcgtccccgg gggcccggcc gggaacggct ggaccgccta tccgccgctc
     3601 tccgctgtgc cggcggcggc gcccggatcg gggctcggcc agtccttgtg gctcctcgcc
     3661 gtcgtgtccc tgtgtctttc atcgaccttt ggcgccatca acttcatcac caccgttttc
     3721 aacctgcgca cgaacggcat gaccccggga cggctgccga tgacggtgtg ggggatcctc
     3781 gtgaccgccg tgctcgcgct gctcacgttt cccgtcctgc tcgccgccgg gctgatgctc
     3841 ctcctggacc gcgttgccgg gaccgcgttc ttcgtgcccc tcgtggtgat cggcggccag
     3901 atccgaggcc aagccggggg cagcccgctg ctgtggcaac atctattttg gttcttcggg
     3961 catccggaag tgtacatcat catcctcccg gcgatgggca ttatctccga cgtgctggcg
     4021 acgttcgccc ggacaccgct cagcggttac cgggcgatcg tgctctcgct cgccgccatc
     4081 ggcttcatca gcttcctcgt gtggggccat cacatgtacg tc
//
LOCUS       DASQGH010000553         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_25264, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000553 DASQGH010000000
VERSION     DASQGH010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25264"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1478
                     /locus_tag="VEW91_10915"
     CDS             63..1478
                     /locus_tag="VEW91_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYM92133.1"
                     /translation="MKMYVGTEWIDKRGSIPVINPYDGSQIDTVPKADADDVERALAT
                     AVRGARAMRKLTGYERSCSLRKAADLMTQRTEDLARTITLEEGKIIGEARVEVTRAVE
                     IIALSGEEAKRLSSEVIPLDGAPGVVNKFGFTIRVPCGVVVAISPFNFPLHLVCHKVG
                     PALAAGNSVVVKPATDTPLSALKLVQILLDAGIPPEAIQCITGSGSEVGEALCRDPRV
                     RKITFTGSRDVGERICQVAGLKKVTMELGSNAPLIVMPDADLDRVAASVAATGYANAG
                     QVCISCQRVLPLKPIYNDFLSVLKPKVAALTTGNPLEEKTKVGPMVRERDAVRVEEWV
                     KEAVQAGARLVTGGERRGAIYAPTVLADVKPEMRVSRSELFGPAVAVTPVGTIDEAIA
                     MANDTNSGLSAGIFTQNLDWAMKFAQEVESGNLHINWGPQWRADLMPYGGLKESGFGK
                     EGPKYAVQEMTELKMVVMHLS"
     gene            1634..2311
                     /locus_tag="VEW91_10920"
     CDS             1634..2311
                     /locus_tag="VEW91_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYM92134.1"
                     /translation="MGYHALRWVIRSLLTLWFGLRVVGEKHVPPRGPVILAANHPSQI
                     DPLILSVAIPRRLTFFAAAELLTMPVLGALVRPFHPVPVRRGHVDLGAIKDCLARLER
                     GGALLIFPEGKISRDGRLQPPHEGLAFIALRSGVPVIPVGVRGTYQVWPLGTRVPHRG
                     RIEVRLGPPIVPDPSAALREGAMPAKEAQAHLTARVMEAIAGLIGDAIDSQAARDPRP
                     HLSHAAG"
BASE COUNT          387 a          790 c          838 g          338 t
ORIGIN      
        1 cagtggggcc gggggcggga gaccgcccac ccggcggggc actcgacggg aggtggagac
       61 ccatgaagat gtacgtcggg accgagtgga tcgacaagcg cggatcgatt ccggtgatca
      121 atccctacga tggctcgcag atcgacacgg tgccgaaagc cgacgccgac gacgtcgagc
      181 gcgcgctggc gaccgcggtg cggggcgcgc gggccatgcg gaagctcacc ggttacgagc
      241 ggtcctgcag ccttcgcaag gcggccgacc ttatgaccca gcgcaccgag gacctcgccc
      301 ggacgatcac cctcgaagag gggaagatca tcggggaggc ccgggtcgag gtcacgcggg
      361 cggtcgagat catcgcgctc tccggcgagg aagcgaagcg cctctccagc gaggtgatcc
      421 cgctcgatgg tgcgccgggc gttgtgaaca agtttggatt cacgatccgg gtgccgtgcg
      481 gcgtggtggt ggcgatcagc cccttcaact tccccctcca cctcgtctgc cacaaggtcg
      541 gcccggcgct cgcggccggc aacagcgtcg tcgtgaagcc ggcgaccgac acccccctca
      601 gcgcgctgaa gctggtccag atcctcctgg atgccgggat cccgcccgag gccatccagt
      661 gcatcacggg aagcgggagc gaggtcgggg aggcgctgtg ccgggatccc cgggtgcgga
      721 agatcacgtt caccgggagc cgggacgtcg gcgagcgcat ctgccaggtc gcgggtctca
      781 agaaggtgac gatggaactg ggcagcaacg cgcccctgat tgtgatgccc gacgcagacc
      841 tggacagagt cgccgccagc gtcgcggcca cgggctacgc caacgccggg caggtctgca
      901 tcagctgcca gcgggtcctt ccactcaagc ccatctacaa cgatttcctc agcgtcctca
      961 aacccaaggt ggcggcgctg acgacgggca accccctcga ggaaaagacg aaggtggggc
     1021 cgatggtgcg ggagcgggac gccgtgcgcg tcgaggaatg ggtcaaggaa gccgtccagg
     1081 ccggtgcccg cctcgtcacc ggcggcgagc ggcgcggcgc catctacgcg cccacggtgc
     1141 tggccgacgt caagccggag atgcgggtgt cgcgcagcga actgttcgga cccgcggtgg
     1201 ccgtgacccc ggtgggcacc atcgatgagg cgatcgcgat ggccaacgac accaactccg
     1261 ggctgtccgc ggggatcttc acccagaacc tcgactgggc gatgaagttc gcccaggaag
     1321 tcgagtcggg caacctgcac atcaactggg ggccccagtg gcgggccgac ctgatgccgt
     1381 acggggggct gaaggagagc gggttcggca aggagggccc caagtacgcc gtccaggaga
     1441 tgaccgagct caagatggtg gtcatgcatc tgtcctagac ccgctgcgcg tccaccggca
     1501 agcctgggtc gggtgccccc taccgggggc accctgcgtt ttcgccccgc gttcgccccg
     1561 ccgcggcgac cggaaggggg gcgaggtcct ctcacgaatg cctgttccca atgccgggct
     1621 gaggctggcc cccatgggat accacgcgct ccggtgggtg atcaggtcgc tcctgaccct
     1681 gtggttcggg ctccgggtgg tcggggagaa acacgtgcca ccgcgcggcc cggtgatcct
     1741 cgccgccaac caccccagcc agatcgatcc gctgatcctc tcggtggcca ttccgcgccg
     1801 cctgaccttc ttcgccgccg ccgagctgct caccatgccg gtgctcggag cgctcgtacg
     1861 gccgttccac ccggtgccgg tcaggcgggg gcacgtcgac ctcggggcga tcaaggactg
     1921 tcttgcgcgc ctcgaacgcg gcggggcctt gctgatcttt cccgaaggaa agatcagcag
     1981 ggacgggcgg ctgcagccgc cgcacgaggg cctcgcgttt atcgccctcc gctccggggt
     2041 gcccgtgatt ccggtcggcg tgcgggggac gtatcaggtc tggccgctcg ggacccgcgt
     2101 cccgcaccgc gggaggatcg aggtcaggct cggccccccg atcgtgccgg atccatcagc
     2161 cgccttgcgg gaaggcgcga tgccggcgaa agaggcgcag gcgcacctca ctgcccgcgt
     2221 gatggaagcg atcgcgggcc tcattgggga cgccatcgat tcgcaggccg cacgcgaccc
     2281 gcgaccgcac ctcagccacg ccgcagggtg agggaagggg gatcttgatg gaccggatct
     2341 ggctcaagca tta
//
LOCUS       DASQGH010000554         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16550, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000554 DASQGH010000000
VERSION     DASQGH010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16550"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VEW91_10925"
     CDS             <1..497
                     /locus_tag="VEW91_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYM92135.1"
                     /translation="QEARESYAAAKAQRDATRAVATKNPDGKTTYTLSLIGSGPKKFT
                     VPRFLPQALTVKAGDTVTWKMEDPTEIHTVTFTSGRKTPPFDILQPPTQPTVAVANPL
                     ALVPTGGTAYDGRSYYNSGIMELITPQGVRSYSLTFTTPGRYQYTCIVHDPIGMRGTI
                     IVTR"
     gene            complement(637..1275)
                     /locus_tag="VEW91_10930"
     CDS             complement(637..1275)
                     /locus_tag="VEW91_10930"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HYM92136.1"
                     /translation="MDKLNVGILLFNQVEVLDFAGPFEVFSRTRLVPGLESRRSEDSA
                     PFLVFTVAASREPVTATAGLQVLPHYAFADAPPIDILVVPGGYGTRALLADSGTLDWI
                     RKAAKEARLVTSVCTGSLLLAKAGLLEERRATTHWGALDLLGSLGSVRVDRDTRVVDD
                     GVVTSAGVASGIDMAFYVVETLFGREVAQETARYIEYPRGVDPGRRVTSGET"
     gene            complement(1306..1749)
                     /locus_tag="VEW91_10935"
     CDS             complement(1306..1749)
                     /locus_tag="VEW91_10935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYM92137.1"
                     /translation="MTPRRGAIERRGAIAGVPEPAGPFSWSVAYGRLLFLSGIRGIDP
                     ATGSPIPGDAERIRLIFAHLARILDAHGASPRDVVSTRVFVTDMGRHRPLVNDAFVRF
                     FGADLPARTIVEVRALNQDDTIELEAIAACGGRARRPGTRRRRRR"
     gene            complement(1751..>2447)
                     /locus_tag="VEW91_10940"
     CDS             complement(1751..>2447)
                     /locus_tag="VEW91_10940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92138.1"
                     /translation="NSGIPLKTVAAMGRYTGMCWASWSNVPLAKPKDLEGRRVSISPS
                     STFFQVWPAFARTFRIDTSKVEVVHADPAARVGLFLSRRLDVMADIFWANDLVILQVR
                     AGEPLNVLKLSDLNFDPLGYLLIVNTPALGRDPDLVKRVTEATLKGFRYTIDHPDEAI
                     AIMTRLFGDRLGAKVIEGQVHNLIPLLNRQPALGKAAQDAWARSLTILYSSGVINRRL
                     ALQDYYTDEFLGG"
BASE COUNT          441 a          815 c          819 g          372 t
ORIGIN      
        1 aacaggaggc gcgggaatcg tacgccgcgg ccaaggcgca acgcgacgca acgcgggccg
       61 ttgccaccaa gaacccggac gggaagacga cgtacacgtt gagcctgata ggttccggac
      121 caaagaagtt caccgtcccc cgcttcctcc cacaggcgtt gacggtcaaa gccggcgaca
      181 cggtcacctg gaagatggaa gatcccacgg agatccatac ggtcaccttc acgtccggca
      241 ggaagacgcc cccgttcgat attctccagc cgccgacgca gccgaccgtc gccgtggcga
      301 acccgttggc gctggtgccc acaggaggga cggcatacga cgggcggagc tactataatt
      361 cggggatcat ggagctcatc acgccccaag gggtccggag ctattcgctg accttcacca
      421 cccccgggcg gtaccagtac acctgcatcg tgcatgatcc gatcggcatg aggggaacga
      481 ttatcgtcac acgctgaagg aagcggtcat gcgcctgggg ttcttcctgc cccaactcgg
      541 acaggccgcc agccccgcac cttttctcca ggcatccaat ccgagggcga cttcctcgga
      601 agcatggagc ggatccgccg gttggtgtga agcgccctat gtttcgcccg atgtaacgcg
      661 tcggcccgga tccaccccgc ggggatactc gatatagcgg gctgtttcct gggcgacctc
      721 acgaccgaac agggtctcga ccacatagaa ggccatgtcg atgccggagg cgacgccggc
      781 ggacgtgacg accccgtcgt caaccacgcg cgtgtcgcga tcgacccgca cgctccccag
      841 ggaccccagc agatcgagcg ccccccagtg ggtcgtggcg cggcgctcct cgagcaggcc
      901 cgccttggcg agcagcaacg agccggtgca gaccgaggtg accagccggg cttctttagc
      961 ggctttgcgg atccagtcca gcgtgccgga atcggcgagc agggcgcgcg tgccgtatcc
     1021 gcctgggaca accaggatgt cgatcggtgg ggcatcggca aaggcgtagt ggggcaggac
     1081 ctgcagtccc gcggtggccg tgacgggttc gcgggaggcg gcgaccgtaa acacgaggaa
     1141 cggcgcgctg tcctcggaac gccgcgactc cagccccggc accaggcgcg tgcgcgagaa
     1201 gacctcgaac ggacccgcga agtccagcac ctcgacctga ttgaacagca ggatgcctac
     1261 gttcagtttg tccatcgacg tcctcctcgt ctcgtgcgcc gaccttcatc ggcgacgtcg
     1321 tctccgcgtc cccggccggc gcgccctccc gccgcaggcg gcaatggcct cgagctcgat
     1381 agtgtcgtcc tggttgaggg cgcggacctc cacgatcgtc cgggcaggca ggtcggcgcc
     1441 gaagaacctg acgaacgcgt cgttcaccag cggccggtgg cgccccatgt cggtgacgaa
     1501 gacgcgggtc gagacgacgt cacgggggga cgcgccgtgg gcgtccagga ttcgggcgag
     1561 gtgagcaaag atgagccgga tgcgctccgc atcgccgggg atgggggatc ccgtggccgg
     1621 gtcgatgccg cggatccctg acaggaagag cagccggccg tacgccacgg accaggagaa
     1681 tggaccggcc ggctccggca cgccggcgat cgccccgcgc cgttcgatcg ccccgcgccg
     1741 gggggtcacc ctacccgccg aggaactcat cggtgtagta gtcttggagt gcgagccgcc
     1801 gattgatgac cccggacgaa tacaggatcg tcagcgatcg cgcccaggcg tcctgcgcag
     1861 ccttgcccag ggccggctgg cggttcagga gcgggatgag gttgtgcacc tggccctcga
     1921 tgaccttggc cccgagccgg tcgccgaaca acctggtcat gatcgcgatc gcctcgtcgg
     1981 gatggtcgat cgtgtaccgg aatcccttga gggtggcctc cgtcacgcgc ttgacgagat
     2041 cgggatcgcg gcccagggcc ggggtgttga cgatcagcag gtacccgaga gggtcgaagt
     2101 tcaggtcgga gagcttcagc acgttcagcg gttcgccggc cctcacctgg agaatcacca
     2161 ggtcgttcgc ccagaagatg tcggccatga cgtccagcct gcggctcagg aacaggccga
     2221 cgcgcgccgc aggatcggcg tggacgacct ccactttgct cgtgtcgatc cgaaacgtcc
     2281 gcgcaaacgc cggccacacc tgaaagaagg tcgatgatgg tgagatcgac acgcggcgtc
     2341 cctcgagatc cttcggcttg gcgagcggca cgttcgacca ggaggcccag cacatgcccg
     2401 tataccgccc catcgccgcc acggtcttga gcgggatgcc gctgttg
//
LOCUS       DASQGH010000555         8790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19436, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000555 DASQGH010000000
VERSION     DASQGH010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19436"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VEW91_10945"
     CDS             complement(<1..454)
                     /locus_tag="VEW91_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92139.1"
                     /translation="MTITIKSISRVLMITVLLAVAALMMGAVYTYRLMPPVPDRVVAT
                     DGTIVYTGADIAAGKKVFQRRSLMDYGTLLGNGGYFGPDFTAEYLEWMIALMQDRYAR
                     EQFGRPFTNLPAPEQAAITSRVGYEWKHSAYRSGAVTVSPVYAAAHREI"
     gene            1018..2331
                     /locus_tag="VEW91_10950"
     CDS             1018..2331
                     /locus_tag="VEW91_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYM92140.1"
                     /translation="MTPKTERVELDRQADLLERAGRYLAGGGLGLFVLPPEVNLVIVE
                     GHGSHVTDARGRDFIDYHLGSGPALLGHAHPEIVAAVARQLAKGSTYYFLNEPVIGLA
                     ERLVNAIPCAEQVHFTGSGTEATFFAMRLARAATGRDKILKFEGGWHGVNDYALWGTV
                     PSRPSRYPDAEPDSLGIPAALRGQVLVAPFNDAARAVEIIDRHAGELAGVIVEPLERV
                     LTPVPGFLEALRDATRRHGIVLIFDEVVTGFRIAWGGAQERYGVIPDLATYGKAMSGG
                     FPMAAVAGPGRIMKFLDARRTERNRLVWASGTLSGNPVSATAGATALEILSRPGTYEH
                     LHKIGGRLRREIHAIGARHGFAAQTPGEDAVFGVRFTSREPLRTWEDLLTADKDLHLR
                     WSIELLRRGILVNPNEKFYISVTHTDADVDRTLEACEEAFAAIAR"
     gene            complement(2247..3209)
                     /locus_tag="VEW91_10955"
     CDS             complement(2247..3209)
                     /locus_tag="VEW91_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HYM92141.1"
                     /translation="MAAHLPEAIALDLVRTLQSGQAFRWRWERLEDGTTAAVGVVGRE
                     IFRVSQDARGTWLLAPEGPDARARLIDYLGLSPGAAPIPRVEAALAADAVIARILPHT
                     RGIGILAQDPWEVLVSFIVSANNNIPKICQSIERLSRLLGAPLGGGAYAFPTPERLAA
                     ARSRTLAACLLGYRAPYVRAAARRVADGRVELAALARLPLAAAREGLLEIPGVGEKVA
                     DCILLFGLRHTSAFPVDTWVRRAVERLYFRGRPRTAREIQAFARDRFGPLAGYAQQHL
                     FCYARAHLRPRPNKPARYRAIAANASSQASSVRSTSASVCVTEI"
     gene            complement(3212..3805)
                     /locus_tag="VEW91_10960"
     CDS             complement(3212..3805)
                     /locus_tag="VEW91_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYM92142.1"
                     /translation="MREEVLVVPRARLFASGEFHGFSSTGIASYLSTIAAHAFFAVRD
                     RVERDPSLKQIIPYLVLRHGDRVLLVRRTQAGSEARLREKFSIGLGGHINPEDVGDAG
                     DPVDAGLRRELAEEVEVPAGWRARPVGVLNDDQEAVGSVHFGIVYIVDLPSPAVRVRE
                     RTKLHGALATLEEVRAVYPGLETWSQFVVDAIDLAEI"
     gene            complement(3864..4343)
                     /locus_tag="VEW91_10965"
     CDS             complement(3864..4343)
                     /locus_tag="VEW91_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HYM92143.1"
                     /translation="MRPIYLRTLCFIRDGDRVLLIRRRKPPNEGLYNALGGKIEPHED
                     PYDAVLREVHEESGLRIREPRLRAIITVITRTTGAQWILFAFVAERPSDPPDPVATDE
                     GDLRWVPLAEVAGLPVVSDIPLMLPHLFAPGGGILMGKIHCDNDDADSILGHEFRFA"
     gene            complement(4348..5154)
                     /locus_tag="VEW91_10970"
     CDS             complement(4348..5154)
                     /locus_tag="VEW91_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM92144.1"
                     /translation="MGRRRRIALPIAGAGVVLSLAFQSPAPGPAEAAAAQGLTPNQLV
                     GVTMAKGIKSGGLFGQGEVTPVGATTSFINTDLPYAVVRVKNLQSETAVTLRVSDPTE
                     PAFSIEEKAPRHRDNLWEEFDFALPLYILGTDLESRTGTWTIQVLFNGQPQSTTAFQW
                     GPATPLALGRIKDLVDLTPAEPDLHWRYGAALALLHHDREGITELQNAIQLDRRYALY
                     YITLGRVYERDGRPADAIRMFQTALSLHGSHYDAVYAGWAQAHLAQLQTR"
     gene            5436..5783
                     /locus_tag="VEW91_10975"
     CDS             5436..5783
                     /locus_tag="VEW91_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYM92145.1"
                     /translation="MPAKQTVTSIKALPAKEIAKGVKIKPLAGDHVMLNYVEFVPGAL
                     VPVHSHHHEQLGLIIEGELEMEIGEERQTLRPGDTYVIPGGIRHGGRAGRAGALVLDV
                     FYPLREDYLKLFA"
     gene            complement(5780..7714)
                     /locus_tag="VEW91_10980"
     CDS             complement(5780..7714)
                     /locus_tag="VEW91_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM92146.1"
                     /translation="MRAAPEIACDVLVAGGGLGGVAAALRAARLGRTVCLLEETGWLG
                     GQISTQGVSHLDEHEHIETFGGTRAYYELRSAIRDYYRSHYVLTPEARRAPRLNPGNA
                     WVSRLSFEPRVGAAVLEAMLARPHGPGDLRIFRHTLAVQAEILEGRIASVLARDTESG
                     ALLRFPPAYVLDATAHGDLFVLPATPYAAGAESRAETGEPSAPERGDPACVQDFTFPF
                     AVEFRPGEVHTVAKPAGYEENRAGQPYSFVAKPRAPGAPAYRLFEQAPGTYGPFFTYR
                     RVLDARNFDDPRVSHDVAIINWPSNDFRGGTLIDRRPDEQALLREQARWLSLGFLYWL
                     QTEAPRDDGGRGYPELRPRPDIMGSADGLSQAPYIREGRRLRARRTIREQDIAQTFHP
                     DARAARFEDAVGIGYYPIDLHGCGEATVSIPTRPFQIPLGALVSVRTANLIPAAKNIG
                     TTRIANGAYRLHPVEWAVGEAAAALADFCQRTRASPQDLLDRPDLVRRLQLRLIDQGI
                     PLYWYDDVPLEHPAFAATQLLATDGIWEGEGGTLHFSPRDVVALGEAKQRVVALARGI
                     RRWGGPGPAEPDAQILRAAPEDAGLPLRWGTALTLVTPAVPGAAPPPHAREDHVTRGD
                     LAVWLGRLLREAIERGAPEI"
     gene            complement(7743..>8790)
                     /locus_tag="VEW91_10985"
     CDS             complement(7743..>8790)
                     /locus_tag="VEW91_10985"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYM92147.1"
                     /translation="VVHQPIGVVAAITPWNFPATMVLRKIAPALAAGCTVVLKPPKET
                     PLTALEIARAFAEAALPAGVFNVVVGKRAAPIAEVFMRDSRVRKIAFTGSTEVGKVLM
                     AQAAAQLKRVAFELGGNAPFIVFDDADLDRAVSNAVAIKYLRVGGQSCICANRIYIQR
                     AIADAFLPRFTEAVRAIKVGSGFEPGVQVGPLINAETLDKVDGFVRDAVAAGARPLTG
                     GRRLTDGAYSKGFFYEPTVLTDVREEMPVARDETFGPVAPVLTFDTDDEVIARANNTP
                     YGLAAYVSSRDLRRVIRVSEALEYGLVCVNDATGYTHEIPFGGFKESGLGREGGRQGI
                     AEYLEVKSISVNIS"
BASE COUNT         1345 a         3000 c         3124 g         1321 t
ORIGIN      
        1 ggatctcgcg gtgagccgcg gcatagacgg gagaaaccgt cacggctccg gaccgatatg
       61 cgctgtgctt ccactcatac ccgacgcggc tagtgatggc cgcctgctct ggcgctggga
      121 gattggtgaa gggtcggccg aattgctctc gggcgtaccg atcctgcatc agcgcgatca
      181 tccattccag gtactccgcg gtaaagtccg gaccgaagta tcccccgttc ccgagcaggg
      241 tcccgtagtc catcaggctg cgccgctgga acaccttctt gccggcggcg atgtcggcgc
      301 ccgtatacac gattgttccg tctgtcgcga ccacacgatc tgggaccgga ggcatgaggc
      361 gataggtgta gaccgctccc atcatcaggg ccgccacggc cagcaggacg gtgatcatga
      421 ggacccgcga gatgctcttg atagtaatgg tcacgcgata ccccttcctg acaaagcatt
      481 atggcatgta cccgtaggcc ctctcttcaa ctcgaccggc gaaccctatg ccggccgacc
      541 gctgtgctac gggaccactt gccggttctg ggggatcttc gtagatgacc ttacagagtc
      601 atcccgctgc ataatccagg gggcaggttt cggcgggggc cctttcggct cctcgatgga
      661 cactgcatat actccacgca aaacgacctg tacaacacca aaccgattcg tacataggga
      721 tcgggatgga cctcgaggat aatcccggca atccgtttct cttagaacac tctcagatgc
      781 atgagcatgg tgtgaggtga atccggcaga ccgctcaatt cacgagcgca ggtagggcgg
      841 cgtccgcggc ctccaactgc tgccgcctta gatctccgtc acgcagttcc tgggccttat
      901 cttcatccct tcatccggcg agcgaggacc ggtctgatgc gaggccgact gatgctccgc
      961 ggaccgcagc atgttatgat tggggcggaa cgcatgcacc gatcaaagga ggcgttcatg
     1021 acccccaaga ccgaacgcgt ggagctggac cgtcaggcgg atctcctgga gcgtgccggc
     1081 cggtatctcg ccgggggtgg gctcgggctc ttcgtgctcc cgcccgaggt gaacctcgtc
     1141 atcgtcgaag ggcacggtag ccacgtgacc gacgcccgcg gccgcgactt tatcgactac
     1201 cacctcggct ccggccccgc gctgctcggg catgcccacc cggagatcgt cgcggcggtg
     1261 gcgcgccagc tcgccaaggg ctcgacctac tacttcctca acgagcccgt catcgggctg
     1321 gcggagcggc tcgtgaacgc gatcccgtgc gcggagcagg tgcacttcac cgggtccggg
     1381 acggaggcga cattcttcgc gatgcggctg gcccgggcgg cgaccggccg ggacaagatt
     1441 ctgaagttcg agggcggctg gcacggggtg aacgactacg cgctgtgggg caccgtgccc
     1501 tcccggccca gccgctaccc cgacgcggag ccggactcgc tcggcatccc ggccgcgctc
     1561 cgcggccagg tgctggtggc cccgttcaac gacgccgcgc gggcggtgga gatcatcgac
     1621 cggcacgccg gggagctggc cggggtgatc gtggagccgc tcgagcgcgt cctgaccccg
     1681 gtcccggggt tcctcgaggc gctccgcgac gccacccggc ggcacggcat cgtgctgatc
     1741 ttcgatgagg tcgtcaccgg tttccggatc gcctggggcg gggcccagga gcggtacggc
     1801 gtcattccgg acctcgccac ctacggcaag gcgatgtcgg gcgggttccc gatggcggcg
     1861 gtcgccggcc cgggccggat catgaagttc ctcgatgccc gccggaccga gcgcaatcgc
     1921 ctcgtgtggg cgagcggaac gctgagcggt aacccggtca gcgccaccgc cggcgcgacc
     1981 gcgctcgaga tcctgagccg gccggggacc tacgagcacc tccacaagat cggcgggcgg
     2041 ctccgccggg agatccacgc gatcggcgct cggcacgggt ttgccgcgca gacgcccggg
     2101 gaggacgcgg tgttcggcgt gcggttcacg agccgggaac cgctgcgcac ctgggaggac
     2161 ctcctcaccg cggacaagga cctccatctt cgctggtcga tcgagctgct ccggcgcggg
     2221 atcctcgtca acccaaacga gaagttctat atctcggtga cgcacaccga cgccgacgtc
     2281 gaccgcacgc tggaagcctg cgaggaggcg ttcgcggcga tcgcgcggta gcgggcgggc
     2341 ttgtttgggc gcggcctgag gtgggcgcgg gcgtagcaga agaggtgctg ctgcgcgtac
     2401 ccggccaggg gcccgaaccg gtcgcgcgcg aacgcctgga tctcgcgcgc ggtccgcggc
     2461 cggccgcgga agtacagccg ttcaacggca cgccggaccc aggtgtccac gggaaacgcg
     2521 gacgtgtgcc gcaggccgaa cagcaggatg cagtccgcga ccttctcgcc cactccgggg
     2581 atctccagca ggccctcccg ggcggccgca agcggcaggc gcgccagggc ggcgagctcg
     2641 acgcgcccgt ccgcgacgcg acgcgcggcc gcgcgcacgt acggcgcccg gtaccccagg
     2701 aggcaggccg cgagcgtccg ggagcgcgcg gcggcgaggc gctcaggggt cggaaaggca
     2761 taggccccgc ccccgagcgg cgccccgagg aggcgggaca atcgctcgat cgactggcag
     2821 atcttgggga tgttgttgtt cgcggagacg atgaatgaga ccaggacctc ccacggatcc
     2881 tgcgccagga ttccgatccc ccgggtgtgc gggaggatcc gcgcgatgac cgcgtcggcg
     2941 gcgagcgccg cctcgacgcg ggggatcggc gcggctcccg gcgagagccc caggtaatcg
     3001 atcagccgcg cgcgggcgtc gggcccctcc ggcgcgagca gccacgtccc ccgggcgtcc
     3061 tggctgacgc ggaagatctc ccggccgacc acccccaccg ccgccgtcgt cccgtcctcg
     3121 aggcgctccc accgccagcg gaaggcctgg ccggactgga gcgtgcgcac caggtccagc
     3181 gcgatcgctt cggggaggtg cgccgccacc gttagatctc cgcgagatcg atcgcgtcta
     3241 cgacgaactg cgaccaggtc tcgagcccgg ggtacaccgc gcggacttcc tcgagcgtgg
     3301 cgagcgcgcc atggagtttg gtgcgctcgc gcacccggac ggcgggcgag ggcaggtcca
     3361 cgatgtagac gatgccgaag tggacgctgc cgaccgcctc ctggtcgtca ttgagcacgc
     3421 ccaccggccg ggcgcgccac cccgcgggga cctcgacctc ctccgcgagc tcgcgccgca
     3481 ggcccgcgtc gacgggatcg ccggcatccc ccacgtcttc cggattgatg tggccgccga
     3541 ggccgatgga gaacttctcc ctgagccgcg cctcggaccc ggcctgcgtc cgccggacga
     3601 gcaggacgcg gtccccgtgc ctgaggacga gatacgggat gatctgcttg aggctcggat
     3661 cgcgctcgac ccggtcgcgc accgcgaaga aggcgtgcgc ggcgatcgtg gacaggtacg
     3721 acgcgatccc ggtcgaggag aaaccgtgga attcgcccga ggcgaacagc cgcgcccggg
     3781 ggacgaccag gacctcttcg cgcatggagg tcagttgtct gggggccgtg cgaactcctc
     3841 cggggccgcg gcccaggcac cgctcatgcg aagcggaact cgtggccaag gatgctgtcg
     3901 gcgtcgtcgt tgtcgcagtg gatcttcccc atgaggatcc cgccccccgg ggcgaagagg
     3961 tgggggagca tgagcgggat atcggacacg acggggaggc ccgcgacctc ggcgagcggg
     4021 acccaccgca ggtctccttc atcggtcgcc accggatcag ggggatcgga cggacgttcg
     4081 gcgacaaacg cgaacagtat ccactgcgcc ccggttgtcc gcgtgatcac cgtgatgatc
     4141 gcccggaggc ggggctcgcg gatccgcagc ccggactcct catggacctc ccggagcacc
     4201 gcgtcgtacg gatcctcgtg cggctcgatc ttcccgccca gggcattgta gagaccttcg
     4261 ttgggaggct tgcgccggcg gatcagcagc acccggtcgc cgtcgcggat gaagcacagg
     4321 gtgcgcaggt agatcgggcg cacggcatca gcgcgtctgc agctgagcca ggtgcgcctg
     4381 cgcccagccg gcgtacaccg cgtcgtagtg actgccatgg agcgagaggg cggtctgaaa
     4441 catccggatc gcgtcggccg ggcgtccgtc gcgctcgtac acccggccga gcgtgatgta
     4501 gtacagcgcg taccgccggt cgagctggat ggcattctgg agttccgtga tcccctcccg
     4561 gtcgtggtgg agaagcgcca gggccgcgcc gtaccgccag tggagatcgg gttccgcggg
     4621 ggtcaggtcc accaggtctt tgattctccc gagggcgagg ggcgtggcgg gcccccactg
     4681 gaacgccgtc gtgctctggg gctgcccgtt gaacagcacc tggatggtcc aggtgccggt
     4741 gcgcgactcc aggtcggtcc ccaggatgta caggggcagg gcgaagtcaa actcctccca
     4801 caggttgtct ctgtgccgcg gcgccttctc ttcgatcgag aacgccggct ccgtgggatc
     4861 gctgacccgc agggtcaccg ccgtctcgct ctgcaggttc ttgacccgaa cgaccgcgta
     4921 cgggagatcg gtgttgatga agctggtcgt ggcccccacg ggggtgactt cgccctgacc
     4981 aaacaggccg ccggacttga tccccttggc catcgtcacc ccgaccaact ggttcggcgt
     5041 gaggccctgg gctgccgccg cctccgcggg gccgggcgcc ggactttgga acgccaaaga
     5101 cagcacgacc ccggcccccg cgatgggaag cgcgatgcgg cgtcgccggc ccatgcgatc
     5161 agcgaaggat caggtggccg gtcgctgcga gaatcaggag acccagcaag acaaagaaca
     5221 cgggatccac gtcctctctc ctctcacccg gtcgcgtgtc gtggcaaaga ggtatgtacc
     5281 ccccggcacg ggggccccaa acgtgcgtgc gaacggtgta ccgaatgggc gttcgctgaa
     5341 cgggggagaa ctcctccacc tgcgcgaatc ctgcacccgc gccagccccg gtggccggtt
     5401 ccggttgggg gacgcgggtg gcgtggaggg gttcgatgcc agccaagcag accgtcacgt
     5461 ccatcaaggc gctgcccgca aaggagatcg cgaagggggt caagatcaag ccccttgccg
     5521 gagaccatgt gatgctcaac tatgtcgagt tcgttccggg ggccctggtg ccggtccact
     5581 cccaccatca cgagcaactg ggattgatta ttgaaggcga gctcgagatg gaaattgggg
     5641 aggagcggca aacgctcagg ccgggcgaca cctacgtgat tcccgggggg atccggcacg
     5701 gcggccgggc cggacgcgcc ggtgccctcg tcctcgatgt cttctacccg ctccgggagg
     5761 attatctcaa gctcttcgct tagatctcgg gcgctccgcg ttcgatcgcc tcgcggagga
     5821 gccgtcccag ccagacggcc aggtccccgc gggtgacgtg gtcctcccgc gcgtgcgggg
     5881 gcggggcggc gccgggcacc gccggcgtca cgagggtcag ggccgttccc caccggagcg
     5941 gtaggccggc atcctccggt gcggcccgga gtatctgggc gtcgggctcc gcaggcccgg
     6001 gcccgcccca ccgacggatg ccgcgcgcca gggcgaccac gcgctgcttg gcctcgccga
     6061 gcgccaccac atcccgggga gagaagtgca gcgtcccgcc ctcgccttcc cagatgccgt
     6121 cggtggcgag gagctgcgtg gccgcgaacg ccgggtgctc gagcggaacg tcgtcgtacc
     6181 agtacagcgg gatcccctgg tcgatgaggc gcagctgcaa tcgcctgacg aggtccgggc
     6241 gatccagcag gtcctgcggg gaggcccggg tgcgctggca gaagtcggcc aatgcggcgg
     6301 ccgcttctcc caccgcccac tcgaccggat gcagccggta cgcgccgttc gcgatgcgcg
     6361 tcgtgccgat gttcttcgcg gccgggatga ggttggctgt ccgcaccgac acgagggcgc
     6421 cgagcgggat ctggaacggc cgggtgggga tgctcacggt cgcctctccg cagccgtgga
     6481 ggtcgatcgg gtagtacccg atcccgacgg cgtcctcgaa gcgcgccgcc cgggcgtcgg
     6541 ggtggaaagt ctgcgcgatg tcctgctcgc ggatcgtccg cctggcacgg agacgccggc
     6601 cttcgcgaat gtacggggcc tgggagaggc cgtccgcgct gcccatgatg tcgggcctgg
     6661 gacggagctc ggggtacccg cgtccgccgt cgtccctcgg cgcctcggtc tggagccagt
     6721 acaggaagcc caggctgagc cagcgcgcct gctcgcggag gagggcctgt tcgtccgggc
     6781 ggcggtcgat cagggtcccc ccccggaagt cgttgctcgg ccagttgatg atcgcgacat
     6841 cgtgggagac ccgcgggtca tcgaagttgc gggcgtcgag cacgcgtcga taggtgaaga
     6901 acgggccgta cgttccgggc gcctgctcga acagccggta ggcgggagcg ccgggggccc
     6961 tgggcttggc gacgaacgag tagggctgtc ccgcccggtt ctcctcgtac cccgcaggct
     7021 tggcgaccgt gtggacctcc cccggccgga actctaccgc gaacgggaag gtgaagtcct
     7081 gcacgcaggc cgggtccccc cgctccgggg ccgagggctc cccggtctcg gcgcgggatt
     7141 ccgccccggc cgcgtagggg gttgcgggca ggacgaacag atccccgtgg gcggtggcgt
     7201 cgagcacgta cgccggcggg aagcggagga gcgccccgct ttcggtgtcg cgggcgagga
     7261 ccgaggcgat ccggccctcg aggatctctg cctggacggc cagcgtatgg cggaagatcc
     7321 ggagatcccc cgggccgtgg gggcgggcca gcatcgcctc cagtaccgcg gcgccgacgc
     7381 gcggctcgaa cgagagccgg ctgacccagg cgttcccggg attcagccgg ggggcgcgcc
     7441 gcgcctcggg ggtgaggacg tagtgtgacc ggtagtagtc gcggatggcg ctgcggagct
     7501 cgtagtacgc gcgggtcccg ccgaaggtct cgatgtgctc gtgctcgtca aggtgggaga
     7561 cgccctgcgt gctgatctgc ccgccgagcc acccggtctc ttcgagcagg catacggtgc
     7621 gccccagccg ggcggcccgg agggcggcgg cgaccccacc caggccgcct ccggcgacga
     7681 gcacatcgca cgcgatctcg ggggcagcgc gcatcgggcg ggcgcaggcc ggcggccccc
     7741 ggtcagctga tgttgaccga gatcgacttc acctccaggt actctgcgat cccctggcgg
     7801 ccgccctcgc ggcccagccc gctctccttg aagccgccga acgggatctc gtgcgtgtat
     7861 ccggtcgcat cgttgacgca gacgagcccg tactccagcg cctcgctgac ccggatcacc
     7921 ctccgcagat cgcgcgagga cacgtacgcc gccagcccat acggggtgtt gttggcccgg
     7981 gcgatgactt cgtcgtcggt gtcgaaggtg agcaccggcg ccaccgggcc gaaggtctcg
     8041 tcgcgggcga ccggcatctc ctcgcggaca tccgtcagca cggtgggctc gtagaagaac
     8101 cccttgctgt aggccccgtc ggtgagccgg cggccaccgg tcaacggccg ggccccggcg
     8161 gcgacggcgt ccctgacgaa cccgtcgacc ttgtcgagcg tctcggcgtt gatcagcggc
     8221 ccgacctgga cccccggctc gaacccggat cccaccttga tcgcgcggac ggcctcggtg
     8281 aacctgggca ggaatgcatc cgcgatcgcg cgctggatgt agattcgatt ggcacagatg
     8341 cacgactggc cgccgaccct gaggtacttg atcgccacgg cattggagac cgcccggtcg
     8401 aggtccgcgt cgtcgaagac gatgaacggg gcgttgccgc ccagttcgaa cgccacgcgc
     8461 ttgagctgcg cggcagcctg ggccatgagc accttgccga cctccgtcga cccggtgaag
     8521 gcgatcttgc ggacgcggct gtcccgcatg aacacctccg cgatcggtgc cgcgcgcttc
     8581 cccaccacga cgttgaagac ccccgcgggg agcgcggcct cggcaaacgc ccgggcgatc
     8641 tcgagggccg tgaggggggt ttccttgggc ggcttgagca cgacggtgca ccccgcggcc
     8701 agcgcagggg cgatcttccg caggaccatc gtggcgggaa agttccaggg cgtgatcgcc
     8761 gcgacgaccc cgatgggctg gtggacgacc
//
LOCUS       DASQGH010000556         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22120, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000556 DASQGH010000000
VERSION     DASQGH010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22120"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VEW91_10990"
     CDS             <1..596
                     /locus_tag="VEW91_10990"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYM92148.1"
                     /translation="REETRRKILESATQVFASKGFHNAAVDDIVKASGTSKGAVYFYF
                     ESKEQIFLSLVEDYASTLAQDFQMAVPKARGLVAQVEAAVVALVRSFQKHRDLAKIVL
                     VDWLSMGPEYQGKRIALKAMLVEVLRGYLDRAVEDGKIAPQDTETAAYAWIGAISEVV
                     LRWLNTGKPDPLEEVLGPLTCLLLRSVGFSVSPAAAR"
     gene            623..1186
                     /gene="pyrE"
                     /locus_tag="VEW91_10995"
     CDS             623..1186
                     /gene="pyrE"
                     /locus_tag="VEW91_10995"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HYM92149.1"
                     /translation="MDRGRVPEAVLADLRARTWAQLSRAAFLRGEYVLSSGRTSPYYI
                     DKYQFETHPDLLRDIARLLAGALPPETTRLVGPALGGVPLATAVALETGIPFVIVRPE
                     AKAHGTARTIEGTLEAGDRVVVLEDVVTTGGQAVRAARAVEAAGGHVLLILAVVDRHE
                     GGREGVERAGYRFEALYDLQEWPIPHG"
     gene            complement(1204..2022)
                     /locus_tag="VEW91_11000"
     CDS             complement(1204..2022)
                     /locus_tag="VEW91_11000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYM92150.1"
                     /translation="MPSTSDLVRTVVQGRVYDLEQPRVAGMPVYPIHRPGYTYHLHRR
                     HRDGYRPEQFGPRTGASGVFLMMDHSGTHVDALSHQASDLRLYGGIEITPQIETPKGF
                     TVHGIETLAPIVTRGVLLDVCAAKQQDPLPEQYRITAADLEAAARLAELTVGPGDALL
                     VRTGYAKLWGDEARYLRAAGVSREGSMWAADKRVRCVGCDNMTWDETEERDPDSGATL
                     FGHLHLIARCGIPIIENLNLEALSRDRRREFLFVCAPLKLVGATGSPVCPIAVA"
     gene            2310..>2822
                     /gene="sufC"
                     /locus_tag="VEW91_11005"
     CDS             2310..>2822
                     /gene="sufC"
                     /locus_tag="VEW91_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HYM92151.1"
                     /translation="MEGALQIKDLVAQVEDKVILKGTSLTVRAGEIHALMGPNGSGKS
                     TLANVLMGYPFYQVVKGEVLYKGEDLLAMAPDERARRGLFIAFQYPVSIPGVTMASFL
                     RTAVSARRGYDKDTLMSLQEFQKLVRSKVEELQVDPQVLGRYVNEGFSGGEKKRGEIL
                     QMAVLEPEIAI"
BASE COUNT          487 a          899 c          983 g          453 t
ORIGIN      
        1 agcgcgaaga aacccgcagg aagatcctcg aatctgctac ccaggtgttt gcttccaagg
       61 gattccacaa cgcggccgtc gacgacatag tcaaggcctc gggaacctcg aagggggcag
      121 tgtatttcta cttcgagagc aaagagcaga tctttctctc cctcgtggag gactacgcct
      181 ctaccctggc ccaggatttc caaatggcgg tgcccaaagc ccggggccta gtcgcccagg
      241 tggaggctgc ggtcgttgcg ctggtccgga gcttccagaa gcatcgggat ctcgccaaga
      301 tcgtcctggt agactggctg tccatgggcc ccgaatacca gggaaagcga atcgcgctca
      361 aagccatgct ggtcgaggtg ctgcgggggt accttgaccg ggccgtcgag gacggcaaga
      421 tcgcccctca ggacaccgag accgccgcgt acgcgtggat cggggcaatc agcgaggtgg
      481 tactgcgctg gctcaacacc ggaaagcccg atcctctcga agaggtgctg ggcccgctga
      541 cctgcctgct gctgcggagc gtgggattct cagtctcacc tgcggcggcc cgttgacccc
      601 cccggcgccg actccctgac ccatggaccg gggtcgggtc cccgaggccg tcctcgccga
      661 tcttcgcgcg cgcacgtggg cgcagctcag ccgggcggcg tttctccgtg gcgagtatgt
      721 gctgagctca gggcggacca gcccgtacta catcgacaag taccagttcg aaacccaccc
      781 ggatctgctc cgtgacatcg cccgcctgct cgccggagcg cttcccccgg agacgaccag
      841 actggtgggg ccggcgctgg ggggagtccc cctggccacc gcggtcgcgt tggagacggg
      901 catcccgttc gtgatcgtcc ggccggaggc caaggcgcac ggtaccgcgc ggaccattga
      961 ggggaccctg gaggccgggg atcgcgtggt ggtgttggag gatgtggtca ccaccggggg
     1021 ccaagccgtg cgggccgccc gggcggtgga agccgccgga gggcatgtgc tcctcatcct
     1081 ggccgtggtg gaccgccacg agggcggccg ggagggggtc gagcgcgcag ggtaccgctt
     1141 cgaggccctc tacgacctcc aggagtggcc catcccgcac gggtgagcga gacccctgcg
     1201 ggtttatgcc accgcgatcg ggcagacggg cgatcccgtg gcgcccacga gtttcagcgg
     1261 ggcacagacg aacaggaact cccggcgccg gtcgcggctc agggcttcga ggttcaggtt
     1321 ctcgatgatg gggatcccgc accgggcaat caggtgaagg tgaccaaaca gcgtcgcgcc
     1381 tgagtccggg tcgcgctctt cggtctcgtc ccaggtcatg ttatcgcacc cgacgcagcg
     1441 gacccgcttg tccgccgccc acatggaccc ttcgcggctc acccccgccg cccgcaggta
     1501 ccgggcttca tccccccaca gtttggcgta gcccgtgcgc acgagcagcg catcccccgg
     1561 cccgacggtc aattccgcga ggcgcgcggc ggcctccaga tccgcggcgg taatccggta
     1621 ctgctcggga agcggatcct gctgcttggc cgcgcacacg tccagaagga cgccgcgggt
     1681 gacaatgggg gccaatgtct cgatcccgtg gacggtgaat cctttggggg tctcaatctg
     1741 gggggtgatc tcgatgccac cgtagaggcg gagatcactg gcctgatgag agagggcgtc
     1801 gacgtgggtg cccgagtgat ccatcatgag gaagaccccc gaggctccgg tccgaggccc
     1861 gaactgctcc gggcggtacc cgtcccgatg cctgcggtgc aggtggtagg tgtagccggg
     1921 gcgatggatg gggtagaccg gcatgcccgc tacccgcggc tgctcaagat cgtagacacg
     1981 cccctgcacc acggtgcgga ccaggtcgga cgtcgacggc atgagcgatc acccccgcct
     2041 gaggagcctg cattgggtat tcggccccgc ggccggacgc cccccttccc tccaaggcgc
     2101 cggcccgccg gcgggagagg ggcccggttc ggcaacttat gcaagcgctg tgttgacata
     2161 cgtgggaccc caccctaaaa tgatagatga gtaatccact ccagaatccc tccgaccgcc
     2221 cggctcctgg gtcgactttg ggcggggacc agcggggcac ggtagtgtag ggggtacagt
     2281 gtagtgtagg gggtacagtg gggtacagta tggaaggcgc actgcagatc aaggatctgg
     2341 tcgcgcaggt cgaggacaag gtcatcctca agggcaccag cctgaccgtg cgggccggcg
     2401 agatccacgc gctgatgggc cccaacgggt cgggcaagag cacccttgcg aatgtactga
     2461 tggggtaccc cttctaccag gtggtgaagg gggaggtgct gtacaagggc gaggacctgc
     2521 tcgcgatggc gcccgacgaa cgcgcacggc gggggctctt catcgccttt caatacccgg
     2581 tgagcatccc cggggtgacg atggcgagct tcctgcgcac ggcggtgagc gcgcgccggg
     2641 gctacgataa ggataccctg atgagcctcc aggagttcca gaagctcgtc cggtcgaagg
     2701 tcgaggagct gcaggtggat ccccaggtcc tgggccgata cgtcaacgaa ggattcagcg
     2761 gaggggaaaa gaagcgcggc gagatcctcc agatggctgt cctggagccc gagatcgcca
     2821 tc
//
LOCUS       DASQGH010000557         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_44917, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000557 DASQGH010000000
VERSION     DASQGH010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44917"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VEW91_11010"
     CDS             <1..678
                     /locus_tag="VEW91_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92152.1"
                     /translation="LQLHEHVPDQHLQSDQLLGGRQLRTQPLMAVRCMSFARLLCAIL
                     LLTTVPLVVACGSSSTASSTTTPSGRSERDAVTNGTITHRPLDGTGGEEVNDDNPGKA
                     DRGNKPAGAQSNPCALVPTAEARAIVGGPITAQEAPLGPTCIYRAAGSKSFVTLAVES
                     VSLASLLPHLRDHKRIAVHGRTGYCGIYGQSMTFVPLGRGHVLNVTAPCGVGRMFAAK
                     ALSRLKT"
     gene            749..>2052
                     /locus_tag="VEW91_11015"
     CDS             749..>2052
                     /locus_tag="VEW91_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HYM92153.1"
                     /translation="MAALAAGGLLPANAAAADTIFGASTPATTDSGDGHSVEIGVKFH
                     SEVAGSVTGIRFYKATTNTGTHIGSLWTASGSLLASATFTSETASGWQQVNFSTPVAI
                     TANTTYIAAYLAPKGHYSDTSSGFASAGVNNPPLSALANSTSANGVYVYSSTSTFPTN
                     TFKATNYWVDVNFEPITASSPGQVTSVSATAAPGSASVTWTAPANGGSPITSYTITPF
                     VGSTAQPATTITGSPPATSTTISGLTNGTTYTFTVTATNAIGTSPPSSPSNAVTPAAA
                     PIAYPDLQVLMPTGEISIIHAGNMRTLEFTHISWNAGAGPLEIHPSYDPLTGISQGFQ
                     ALDTSPSPGVWTFDHTVPIVGPMIWDPPSDYRFPLDRFWLYNTASGGGVGSLVAASPK
                     VDFCMTSDVFVGGVPNTPTRNGYPSGACSSPEGTLGLSVGWG"
BASE COUNT          402 a          754 c          586 g          310 t
ORIGIN      
        1 ctacagctcc acgagcacgt tcccgaccaa caccttcaaa gcgaccaact actgggtgga
       61 cgtcaacttc gaacccagcc actgatggct gttcgctgca tgtccttcgc ccgactacta
      121 tgcgcgatct tgctcctgac cacggtgccg ctcgtggtgg catgcggcag ctcctcaacg
      181 gcaagctcga ccacgacgcc gtccggccgg agcgaacgtg acgcggtgac caacggcacg
      241 atcacccacc gtccattgga cgggaccggt ggcgaggaag tcaacgatga caatcccggc
      301 aaggcggatc gcggcaacaa gcccgctgga gctcagagca atccatgcgc gctcgtccct
      361 accgccgagg cgcgggcgat cgtcggcgga ccgatcacgg ctcaggaggc gccgctggga
      421 cccacgtgca tctaccgggc ggcgggctcc aagagcttcg tcacgctcgc cgtcgagtcg
      481 gtcagcctcg cgagcctgct gccgcatctc cgcgatcaca agcggatagc ggtgcacggc
      541 cgcaccgggt actgcggcat ctatggacag tcaatgacat tcgtcccgct cggacgcggc
      601 cacgtcctga acgtcaccgc tccctgcggg gtgggcagga tgttcgccgc caaggctctg
      661 tcgagactca agacctgagg gttgcacgca tgaggcaagg agagctaccg tgacaagaat
      721 gacacgtgct tacgcactgg catggacggt ggcagcgctg gcagcggggg ggcttctgcc
      781 cgccaacgcc gccgcggcgg acacgatctt cggcgcctcg accccggcca caaccgactc
      841 cggtgacggg cactcggtcg agatcggcgt gaagttccac tcggaagtgg ccggcagcgt
      901 caccggtatc cgcttctaca aagccaccac caacaccggc acgcacatcg gcagcctgtg
      961 gactgccagc gggtcgctgc ttgcatcggc gacgttcacg agcgagacgg cctcgggctg
     1021 gcagcaggtc aacttctcaa cacccgtggc gatcaccgcc aacaccacct acatcgccgc
     1081 ctacctagcg cccaaaggac actactccga cacctcctcc ggattcgcct cagccggagt
     1141 caacaaccca cccctatccg ccctcgccaa ctccaccagc gcgaacggcg tatacgtcta
     1201 cagctccacg agcacgttcc cgaccaacac cttcaaagcg accaactact gggtggacgt
     1261 caacttcgaa cccatcacgg caagctcccc gggtcaagtc accagcgtga gcgcgaccgc
     1321 ggcccccggg tcggcctccg tgacgtggac cgccccggcg aacggcggta gcccgattac
     1381 gagctacacg atcacccctt tcgtaggctc gacggcccag ccagccacaa cgatcaccgg
     1441 ctccccgccg gcgaccagca ccacgatcag cgggctaacg aacggtacca cctacacctt
     1501 taccgtcacc gccaccaacg ccatcggcac gagcccgcca tcctcgcctt cgaatgccgt
     1561 caccccagcg gccgcgccga tcgcctaccc ggatctgcag gtgttgatgc cgaccgggga
     1621 aatctcgatc attcatgccg ggaatatgag gacgctcgag tttacgcaca tcagttggaa
     1681 tgctggcgcg ggtccgcttg agatccaccc cagctatgac ccccttacgg gcatctcaca
     1741 gggcttccag gcactcgaca cgagccccag cccaggagtt tggacgttcg accacaccgt
     1801 cccgatcgtc gggccgatga tctgggaccc accaagcgac taccgcttcc cgctggatag
     1861 gttctggctc tacaacacgg cctccggcgg cggagtagga agcctcgtcg cggcgagccc
     1921 caaggtcgac ttctgcatga cctccgacgt cttcgtcggc ggagtgccca acacacccac
     1981 ccgcaacggg tacccgagcg gcgcatgctc ctcacccgaa ggcaccctcg gcctgagcgt
     2041 cggctggggc ga
//
LOCUS       DASQGH010000558         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33605, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000558 DASQGH010000000
VERSION     DASQGH010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33605"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1969)
                     /locus_tag="VEW91_11020"
     CDS             complement(<1..1969)
                     /locus_tag="VEW91_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYM92154.1"
                     /translation="MGRTSEHTPVVAALARQGRAGIVFHNVPQNPHYVAYAPLPGYPG
                     WAVDVEQPRDLVLALPHGLQNRMALLGVVIVAGMSLLAFADVRRVVRPLARLREAAEQ
                     IAGGDLDHPVQVDRKDEVGTLARTFEAMRSKLKTSREEIAAWNRELEDRVAARTRELQ
                     AMAEENARLFGVARREERNLASTVDLLSDGLLTLTLDRRILSLNPAAERLLGWTSEEA
                     GGLECTKLLACPGCLDGQARTPCFLEAALESGRTVRGIRTHARRRSGNAFPVSLTAGL
                     GRDETDRPTHFIVTITDMTTEEAYEQQLRKQIRQLSALHEVAQALNLLPLRTVEETSQ
                     DIVQRIAAATGGRCRLDLDPGGDGPPETRAASTGSQDGGDGLLHAGLVGEPLVIQGRR
                     IGTLWLDPGPTGFTEGNPTLVSIIASQVAVAVENAKLYEEVRDRGALRRQLLERIVTA
                     QEEERHRIARELHDEIGQALTALVMQLGAITAAPPLDAAALRNRLAGIREMTSQTIGE
                     VRRLMLNLRPAVLDDLGLVPAIRWYAEAYLRPAGVEARVSVVGLEEHQRLPGHLELVA
                     FRLTQEALTNVLRHARATHVTITLERKNGTLALSISDNGCGFDVEKRRMPGQRGGWGL
                     VGMRERAALLGGSVAVTSHPGHGTHVTVMIPI"
BASE COUNT          341 a          753 c          729 g          353 t
ORIGIN      
        1 caatcgggat catcaccgtg acatgggtac catggcccgg gtgagaggtc acggccacgc
       61 ttccccccag cagggccgcg cgctcgcgca tcccgaccaa tccccatccg ccccgctgcc
      121 ccggcatccg ccgcttctcg acgtcaaacc cgcacccgtt gtcgctgatc gagagggcga
      181 gcgtcccgtt cttccgttcg agcgtgatcg tgacatgggt ggcccgggca tgacgtagaa
      241 cgttcgtgag cgcctcctgg gtcaggcgga acgccacgag ttccagatgc ccaggaagac
      301 gctggtgctc ctccagcccg accacagaca cccgagcctc gacgcccgcc ggacggaggt
      361 acgcctcagc gtaccaccgg atggccggca cgaggcccag atcgtcgagg acggccgggc
      421 gaaggttcag catcagccgg cgcacttccc cgatggtctg ggacgtcatc tcccggatcc
      481 cggctagccg gttccgcagc gccgcggcat caagtggggg ggccgccgtg atcgccccca
      541 gctgcatcac gagggccgtc agcgcctgac cgatttcgtc gtgaagctcg cgggcgatcc
      601 ggtgacgttc ctcctcctgg gccgtgacga tccgttccag gagctggcgg cgcagcgcgc
      661 cccggtcccg gacctcttca tacagcttcg cgttctccac ggccacggcg acctggctgg
      721 cgatgatgct gactagggtg gggttcccct cggtgaaccc ggttgggccg gggtcgagcc
      781 acagcgtgcc gatccggcgc ccttggatga ccaggggttc gccgaccaga ccggcgtgca
      841 agagtccatc ccccccgtcc tggctccccg tcgaggctgc gcgggtctcc ggcggcccgt
      901 cgccgccagg gtcgagatcg agacggcacc ggcctccggt ggcagcagcg atcctctgca
      961 caatgtcctg ggacgtttct tccaccgtcc gcagggggag cagattgagc gcctgcgcga
     1021 cctcgtgaag agcgctgagc tgccggattt gcttgcggag ctgctgttcg taggcctcct
     1081 ccgtcgtcat gtcggtgatg gtgacgatga aatgcgtggg gcggtccgtc tcatctcgtc
     1141 ccaggccggc ggtcaaggac acgggaaacg cgttcccgct gcggcggcgg gcgtgggtgc
     1201 ggatgcctcg gacggttcgt cccgactcca gtgctgcctc aaggaagcag ggggtcctcg
     1261 cctggccgtc gaggcagcct gggcaggcta gtagctttgt gcactccagc cctccggcct
     1321 cctcgctcgt ccacccaagg agacgctcgg cggcgggatt gagggagagg atgcggcggt
     1381 ccagggtcaa ggtgagcagt ccgtcactga ggaggtcgac cgtcgaggcg aggttgcgct
     1441 cctcgcgccg ggcgacccca aataaccggg cattctcttc agccatcgcc tgaagttctc
     1501 gcgtccgtgc ggcaacgcga tcctcgagtt cccgattcca cgcggcgatc tcctcgcgcg
     1561 aggtcttgag tttcgaccgc atcgcctcga aggtccgcgc aagcgttccc acctcgtctt
     1621 tccggtctac ctggaccggg tggtccagat cccctcccgc gatctgctcc gcagcctcgc
     1681 ggaggcgggc cagcggccgg acgacacgcc ggacatccgc gaacgcaagg agcgacatac
     1741 ccgcgacgat gaccactccc aacagcgcca tacggttctg caggccgtgc gggagggcga
     1801 gcaccagatc gcgcggttgc tcgacatcga ccgcccatcc cggatatcca ggcaatgggg
     1861 cgtaggcgac gtagtgggga ttctggggga cgttgtgaaa cacgatgcct gcccggccct
     1921 gccgggcgag cgcggcgacg accggggtat gctcgctcgt tcgccccacc tcgttctgct
     1981 ccgaggatgc cagaaccgtt ccttggccta cgagttcgat gccgagcggc acggcgccgt
     2041 gcccgcgaag agcgtctgcg acccccgcct gcgagaggtc aagagcgccg acaaggagat
     2101 cgccagaccc ctggagaggc agcacaaacg ccgccaggtg ggttccaggg agcaccgtca
     2161 gggcaggagg atcagg
//
LOCUS       DASQGH010000559         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30892, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000559 DASQGH010000000
VERSION     DASQGH010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30892"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VEW91_11025"
     CDS             <1..533
                     /locus_tag="VEW91_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HYM92155.1"
                     /translation="PRIAPTAFIAPTATVIGDVTVGDEASIWFGAVLRGDVGRIEVGP
                     RANVQDNAVLHSTDRVPSIVGEGVTVGHGAVLEGCTVHRGALVGMNAVVLHEAVVGED
                     ALIGAGSVVTDGTQIPPRTVAAGAPCRVRKQLSGASAAWIAGAARAYVGLSRRYKEAQ
                     DRERRFQSAAEGPVPG"
     gene            complement(542..1516)
                     /locus_tag="VEW91_11030"
     CDS             complement(542..1516)
                     /locus_tag="VEW91_11030"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYM92156.1"
                     /translation="MPQMTYLEAIRDGLLWEMRRDPRVFVIGEDIGTYGGAFGITKGF
                     LEEFGPWRVIDAPLAETANVGAGIGAAAMGMRPVVELQFADFVSDAFTQVVNVAAKFH
                     WRTGCPLPLVIRMPYGGDIKGGPFHSQCMEAWFVHTPGLKVVAPAFADDAKGLLLAAI
                     RDPNPVMFFEHKHLYRYQREEVPPGEHLTPIGKSRTVREGSHLSVFSYGWMLHKCLAA
                     AETVGAEGIAADLIDLRSLAPLDRQAILASVRKTGKALIVHEAVRTGGIGGEIAAIIA
                     EEAFDALDGPVMRLASEDTPVPHSPVLEAAFQPQVDEIARAMRRLAAY"
     gene            complement(1539..>1887)
                     /locus_tag="VEW91_11035"
     CDS             complement(1539..>1887)
                     /locus_tag="VEW91_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM92157.1"
                     /translation="VLAVYGAAREAVARARGGGGPTLIECRTMRVRGHSEADTFSYVP
                     KALLAEWATRDPIALFERRLRTDGHLPPEADRALTERIATEVEDALAWAEANPEPAPE
                     SVAEGVYAEGGET"
BASE COUNT          265 a          690 c          666 g          266 t
ORIGIN      
        1 cccccaggat cgcgccgacc gcgtttatcg cgccgaccgc gaccgtgatc ggcgacgtga
       61 cggttgggga cgaggccagc atctggttcg gggcggtgct ccgcggagac gtcgggcgga
      121 tcgaggtggg cccgcgggcg aacgtccagg acaacgcggt cctccacagc accgaccggg
      181 ttcccagcat cgtcggcgag ggggtcacgg tcggccacgg tgccgtgctc gagggctgca
      241 ccgtccaccg gggggcgctg gtcgggatga acgcggtcgt cctccacgag gcggtggtcg
      301 gcgaagacgc gctcatcggc gcggggagcg tcgtcaccga cgggacacag atcccgccca
      361 gaaccgtcgc ggccggcgcg ccctgccggg tgcgcaaaca actctcggga gcgtcggccg
      421 cgtggatcgc cggggcggcg cgcgcgtatg tggggctctc ccggcgctac aaggaggcgc
      481 aggatcggga gcggcggttc cagagcgcgg ccgaggggcc ggtgcccggc taggcgcgcc
      541 ctcagtaggc cgcgagccgc cgcatggccc gggcgatctc gtcgacctgg ggctggaacg
      601 ccgcttccag caccgggctg tgcggcaccg gggtatcctc gctcgcaagc cgcatcaccg
      661 gcccatcgag cgcgtcgaac gcctcttcgg cgatgatcgc cgcgatctct cccccgattc
      721 ccccggtccg gaccgcttcg tggacgatca gcgccttgcc ggttttcctc accgaggcga
      781 gtatcgcctg tcggtcgagc ggggccaggc ttcggaggtc gatcaggtcg gctgcgatcc
      841 cttccgctcc taccgtctcg gccgccgcga ggcacttgtg gagcatccac ccgtacgaga
      901 acaccgagag gtgcgacccc tcgcggacgg tccgggactt cccgatcggg gtaaggtgct
      961 cccccggggg cacctcctcc cgctggtacc ggtagaggtg cttgtgctcg aagaacatca
     1021 ccgggttggg gtcccggatc gcggcaagca gcagcccctt ggcgtcgtcc gcgaacgccg
     1081 gggccacgac cttgagcccg ggcgtgtgga cgaaccaggc ctccatgcac tgggagtgga
     1141 acggcccgcc cttgatgtcg ccgccgtagg gcatccggat cacgagcggg agcgggcacc
     1201 ccgtccgcca gtgaaacttc gccgcgacat tgacgacctg ggtaaacgcg tccgagacaa
     1261 aatccgcgaa ctgcagctcc acgaccggcc gcatccccat ggcggcggcg ccgatcccgg
     1321 cccccacgtt ggcggtctcg gccagcgggg cgtcgatgac ccgccagggg ccgaactcct
     1381 cgaggaaccc cttcgtgatg ccaaacgcgc cgccgtacgt cccgatgtcc tccccgatca
     1441 cgaacacgcg ggggtcgcgc cgcatctccc agagcaagcc gtcgcggatg gcctccaggt
     1501 aggtcatctg gggcatggca ccgcctcgac gccgggcgtc atgtctcgcc gccctccgcg
     1561 tacaccccct ccgccaccga ctcgggcgcc ggctcggggt tcgcctcggc ccaggccagg
     1621 gcatcctcga cctcggttgc gatccgctcc gtcagcgcgc gatcggcctc gggcggcagg
     1681 tgcccatctg tccgcaggcg gcgctcgaac aacgcgatcg gatcgcgcgt cgcccactcg
     1741 gcgagcaggg ccttgggcac gtaggagaag gtgtctgcct cgctgtggcc gcgcacccgc
     1801 atcgtgcggc actcgatgag ggttgggcca ccgccccccc gggcgcgggc gacggcctcg
     1861 cgcgccgctc cgtacaccgc caggacg
//
LOCUS       DASQGH010000560         7017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19444, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000560 DASQGH010000000
VERSION     DASQGH010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19444"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1422)
                     /locus_tag="VEW91_11040"
     CDS             complement(139..1422)
                     /locus_tag="VEW91_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HYM92158.1"
                     /translation="MSADAALLAAARAVLRANDLGEYTRPSPRLYPHQWNWDSAFAAI
                     GWAHVDWPRAVREVDALLAGQWVNGMLPQIRYSPAVTGYFPGPEMWPAPRVRRQGERT
                     SGISQPPILPTAVYLAGLLQPVERVRLEWWARIYDPLCAMLRYFPRHRTGAGSPLIAI
                     VHPWESGLDNNPRWDLAVSRGHRPTRDYRRTDTAVVAPSMRPTAADYDLYLYLVEQLV
                     ANGYEIGPEIGTAPFAVYDALFNAIWYRAAIDLHRIAQALGHPPVVPAAHLREFRDAY
                     HATLWNDAAGLFRDFDVPSGAQIPVDTVAGLGAIWGGLVEADQATAMLARYRARSPGC
                     RLIPSTPPDQAGFDPARYWRGPVWANTNWLLARGLEALGLRDAARALAEATLDLIRSA
                     GIREYYHAYTGEGLGGSEFSWTAALGIDLLLRPIV"
     gene            complement(1412..2512)
                     /locus_tag="VEW91_11045"
     CDS             complement(1412..2512)
                     /locus_tag="VEW91_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92159.1"
                     /translation="MASIRLDQVRKIYGTVVAVDGVSLEIADGEFVVLLGPSGCGKTT
                     ILRCVAGLEVVDGGRVVIGGRDVTASPPAARGIAMVFQSYAVFPHMTVFDNIAFGLRM
                     RRLPPHEVNGRVAEGAALLQIDPLLTRYPAQLSGGQRQRVAVARALVMRPEVLLMDEP
                     LSNLDALLRLQMRAELKRLHQETRSTTVYVTHDQAEALSLGERVAVMREGKIVQCDTP
                     ARVYDAPASRFVGGFIGSPPMNFLRGTVRSLDGQVAIELDGATIPLGRRAAPGVPQGA
                     GVLLGIRPEHIALEHEHRPDALPGRILVVEPTGPHQLLTVHAAGEPLKVIAPPDARVA
                     PAEQVWLGFSPARIRLFDPGTGEAIDARGDER"
     gene            complement(2552..3373)
                     /locus_tag="VEW91_11050"
     CDS             complement(2552..3373)
                     /locus_tag="VEW91_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYM92160.1"
                     /translation="MTPAARRALLAVGVTLIAAWVLLPLALIAMASLTTRTQLYTWPR
                     SFVPQPWSLETVRYFLRFPGVLPSTGRSLAVAAVTILLSVLIGAPAGYAISRFRFPGR
                     EGVLLGILATKMFPATILAVPLAVAFITWGLYDTVFGVALVHTALALPFTIAITTSVF
                     SGVPGDLEEAAMTLGCSRLQAFRRIALPIALPGLAAATIFTFVLSWNEVFAATILTVR
                     NRTLPALVMNAVGALGAGAPLDYRFAAGFFMVIPALVIIFAIRRYLLTLWGVTVR"
     gene            complement(3370..4275)
                     /locus_tag="VEW91_11055"
     CDS             complement(3370..4275)
                     /locus_tag="VEW91_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM92161.1"
                     /translation="MTVGARTPERPAPVPVRAKVDWLPYALLAPTVVFLLVFFVYPMV
                     QALVLSVRRSDGSLTLGYFQAMLADTYFAEALRNSIILTAIAIPLQVALALVIALLVN
                     ARFRGHAGFLYLCAIPLGISDLAAGLIWLSIFTERGYLNAALRALGYVAQPVTFLSIE
                     HPNRLYAAIIATEVWRATAIVMIVLVAGFQLIPRDYFEAADVFGATGWRKLRHVTLPL
                     LRPALQTALIIRTILAFQLFATIITLAGRIVPVLAGEAYFQYSLYRNPNIASAYAVVI
                     MVLSMAVIVAYLWLFRAREEEAARS"
     gene            complement(4313..5620)
                     /locus_tag="VEW91_11060"
     CDS             complement(4313..5620)
                     /locus_tag="VEW91_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92162.1"
                     /translation="MRRWTAAGLLTTLLVLGAGVPPSPAPVQQITFTSTQLNPVQEQD
                     WARTVLLKGFEDEAGIRIDFVSDTAGPYIDRLLAEAKAGRGSVAVTGTLQGDFPLLVA
                     EGAVRDVAPLLRQLEGRKDRSFAADLVRAGQIGGIQAFVPWMQATYLIVANKQAMPYF
                     PRGRNPQAMTYDDLLQWGENIKQATGQRRLGFPAGPAGLYGRFLHGYAYPSFTGSQVK
                     RFKSPDAVAMWQYLRRLWGLTHPSSFVYEFMSEPLLLGEVWVAWDHVARLLPALRERP
                     NDFVVLPSPAGPRGRSYLAVIVGLGIPKNSPASDVGERLIEYLTRPRTQVMTLQGVAF
                     FPTVREASGTLPTGGLRAVAEGVAAQATAPDAHTALLPIGLGAQSGQFVPLYVDTFRQ
                     IAVQGKPIDGVLAAQAGKLEELFRVTNAPCPAPDPPQRPCRPD"
     gene            complement(5804..6802)
                     /locus_tag="VEW91_11065"
     CDS             complement(5804..6802)
                     /locus_tag="VEW91_11065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92163.1"
                     /translation="MSGLTRRELLTTAACTILGASTLTRAEAATASEILRVIAFPGGF
                     NLPLWVAQEKGLFAARGLTVQLTPTPSSVFQLTHLITGEFDIAMTAIDNVIAYDAGEG
                     EAAVPGRPDLFAFMGGDNGFLRLIVKPEIQTYADLRGQALSVDAVSTGYAFVLRKMLE
                     INGLGPNDYALVPAGGVLQRWQALVAGTHAGTLLVTPFEVLARQRGLRLLGNAIDVLH
                     RYQGVVGAARRSWAASHRDQLVRFIRAYRDALLWLYTPENRAEGIQILARNANFPPEL
                     APQAFEILVDPFNGFAPAAAPDFEGLRMVLALRNQYAAHRVPDNPLAYVDLRYYAA"
BASE COUNT         1170 a         2437 c         2440 g          970 t
ORIGIN      
        1 ccttcccaag cgctcgggag cggctccagc gccagatccg gtctcgcgag gcagtgagga
       61 gcgagttctc cgcctcatgg cggactcctg caccttgatc gcatcgccgc actgcgcgcg
      121 aggtcggaca gcccagggtc agacgatcgg ccggaggagc aggtcgatcc cgagggccgc
      181 ggtccacgag aactcggatc cgcccagacc ctctcccgtg tatgcgtggt agtattcgcg
      241 gatcccggcc gatcggatca ggtccagcgt ggcctccgcc agggcccgcg cggcgtcgcg
      301 caatcccaga gcttccaacc cgcgggcgag cagccagttc gtattggccc agacggggcc
      361 acgccagtac cgggcgggat cgaaccccgc ctgatccggc ggcgttgacg ggatcaggcg
      421 acacccgggg ctgcgcgccc gataccgggc aagcatcgcc gtcgcctgat cggcctccac
      481 gaggccgccc cagatggcgc ccaggccggc gacggtgtcg accgggatct gcgccccgct
      541 cgggacgtcg aagtcccgga acagtccggc ggcgtcgttc cagagcgtcg cgtggtaggc
      601 gtcgcgaaac tcgcgcaggt gcgcagcggg cacgaccgga gggtgcccca gcgcctgggc
      661 gatccggtgg aggtcgatgg ccgcgcgata ccagatggca ttgaacagcg cgtcgtacac
      721 ggcaaagggt gcggtgccga tctccgggcc gatctcgtag ccgttcgcga cgagctgctc
      781 gaccaggtac aggtacaggt cgtagtcggc ggccgtcggc cgcatcgagg gggccacgac
      841 cgcggtatct gtccgccggt agtctcgggt cggccggtgc cccctgctca ccgcaaggtc
      901 ccaccgcgga ttgttgtcga ggccgctctc ccacgggtgt acgatcgcga tcagcggcga
      961 accggcgccc gtccggtggc gcggaaagta ccggagcatc gcacacagcg gatcatagat
     1021 gcgcgcccac cactcgagcc gcacccgttc gacgggctgg agcagccccg ccaggtagac
     1081 cgctgtcggg aggatcggcg gctgcgaaat cccgctcgtc cgctcgccct ggcggcgcac
     1141 ccgcggggcc ggccacatct ccggcccggg gaagtagccg gtgacggcgg gactgtagcg
     1201 gatctgcggc agcatgccgt tgacccactg cccggccagg agcgcgtcga cctcgcgcac
     1261 cgcccgcggc cagtcgacgt gagcccaccc gatcgccgca aaggcgctgt cccagttcca
     1321 ctggtgcgga tacagccgag ggcttggccg tgtatactcg ccgaggtcgt tggcccgcag
     1381 gacggcccga gccgcggcga gcagagcggc atcagcgctc atcgccgcgg gcgtcgatcg
     1441 cctccccggt gcccggatcg aacagccgga tgcgcgccgg gctgaacccg agccagacct
     1501 gctccgccgg cgcgacgcgc gcgtccggcg gcgcgatcac cttgagcggc tccccggccg
     1561 cgtggaccgt cagcagctga tggggcccgg tcggctccac cacgaggatg cggccgggca
     1621 acgcatccgg ccgatgctcg tgctcgaggg cgatgtgctc cgggcggatc ccgagcagca
     1681 cccccgcgcc ctgcggcacg cccggcgcgg cgcgccggcc gagggggatc gtcgcgccat
     1741 ccagttcgat cgcaacctgt ccgtcgagac tccgcaccgt cccccggagg aagttcatcg
     1801 gcgggctccc gatgaagccg cccacaaacc ggctggccgg ggcatcgtac acgcgggcgg
     1861 gggtgtcgca ctggacgatc tttccttccc gcatgacggc gacgcgctcc ccgaggctca
     1921 gcgcctccgc ctgatcgtgc gtgacataga ccgtggtgga ccgggtctcc tggtgcagcc
     1981 gcttcagctc ggcgcgcatc tgcaaccgca gcagcgcgtc gaggttgctg agcggctcgt
     2041 ccatcagcag aacctcgggc cgcatgacaa gcgcccgcgc gaccgccacg cgctgccgct
     2101 ggccgcccga gagttgcgcc gggtaccggg tgagtagggg atcgatctgc aagagcgcgg
     2161 cgccctcggc cacccggccg tttacctcgt gcggcggcag gcgccgcatg cgcagtccaa
     2221 acgcgatgtt gtcgaacacg gtcatgtggg ggaagaccgc atagctctgg aagaccatgg
     2281 cgatcccgcg ggccgcgggc ggcgaggccg tcacgtcgcg gccgccgatc acgacgcggc
     2341 cgccgtcgac gacttccaac ccggccacgc aacggaggat cgtcgtcttg ccgcagccgc
     2401 tcggacccag cagcaccacg aactccccgt cggcgatctc cagggagacg ccgtcgaccg
     2461 ccaccacggt gccgtagatc tttcgcacct ggtccaggcg aatggacgcc acgtcaggcc
     2521 gccccccgcg gggcacgctc cgccggtgcc gtcaccgcac cgtgactccc cacaatgtca
     2581 gcaggtatcg ccggatcgcg aagatgatca cgagcgccgg aatgaccatg aagaagcccg
     2641 cggcgaaccg atagtccaac ggcgcccctg caccgagggc cccgacggcg ttcatgacca
     2701 gcgccggcag ggtccggttc cgcacggtga ggatcgtcgc ggcgaacacc tcgttccacg
     2761 agagcacgaa cgtgaagatc gtggcggcgg ccagcccagg cagcgcgatc ggaagggcga
     2821 tccggcggaa cgcctgcagc cggctgcacc ccagcgtcat agcggcctcc tccaggtccc
     2881 ccggcacgcc gctgaacacg ctggtcgtga tggcgatcgt gaacggcagg gcgagcgccg
     2941 tatgcaccaa cgcaacgccg aacacggtat catacagccc ccaggtgatg aacgccacgg
     3001 ccagcggcac ggccagaatc gtggccggga acatcttggt cgcgaggatg cccaggagga
     3061 ccccctcccg gcccgggaac cggaacctcg agatcgcgta cccggcaggc gccccgatga
     3121 gcaccgacag cagaatcgtc accgcggcaa cggccaggct gcgcccggtg gacggcagga
     3181 cccccgggaa ccgcaggaag taacggaccg tctcgaggga ccacggctgc gggacgaacg
     3241 atcgcggcca cgtatacagc tgcgtgcgcg tggtcaggct ggccatcgcg atcagcgcca
     3301 gcggcagcag cacccacgcc gcgatcaagg tgacgccgac ggcgagcagg gcgcgtcggg
     3361 cggccggcgt cacgatcggg cggcctcctc ttcccgggcg cggaacagcc acaggtaggc
     3421 cacgatcacc gccatcgaca gcaccatgat caccacggcg tacgcgctgg cgatgttggg
     3481 gttgcggtac agggagtact ggaagtacgc ctctcccgcg agcacgggca cgatccgtcc
     3541 cgccagggtg atgatcgtcg caaacaactg gaacgccaga atcgtgcgga tgatcaacgc
     3601 cgtctggagc gccggccgca gcagggggag cgtgacgtgg cggagtttcc gccatccggt
     3661 ggcgccaaac acgtccgccg cttcgaagta gtccctgggg atgagctgga agccggccac
     3721 gagcacgatc atgacgatgg ccgtggcgcg ccagacttcc gtggcgatga tcgccgcgta
     3781 gagccggttg gggtgttcga tcgagaggaa cgtcacgggc tgggccacgt accccagcgc
     3841 tcgcagggcc gcgttgaggt agccgcgctc cgtgaagatc gagagccaga tcagcccggc
     3901 cgccagatcg gagatgccca gcgggatggc gcagagatag agaaaccccg cgtggccgcg
     3961 aaagcgcgcg ttgaccagca gcgcgatcac gagcgcgagg gccacctgga gggggatggc
     4021 gatcgcggtc aggatgatgc tgttgcggag ggcctccgcg aagtacgtgt ccgcgagcat
     4081 ggcttggaaa tagccgagcg tcaggctgcc gtcgctccga cggacgctga ggacgagggc
     4141 ctgcaccatc gggtagacga agaacacgag caggaagacg accgtcggcg cgaggagtgc
     4201 gtacggtagc cagtccacct tcgcccggac cggcaccggc gccggacgct ccggggtccg
     4261 cgcccccacc gtcatcgggc agcccccgtc gatccccgcc tagcgcgggg cttcagtccg
     4321 gccggcaggg acgctgcggt gggtcggggg ccgggcacgg cgcgttcgtg acgcggaaca
     4381 gctcctcgag cttcccggcc tgagcagcga gcacgccatc gatgggtttg ccctgcacgg
     4441 cgatctggcg aaacgtgtcg acgtagagcg gcacgaactg ccccgactgg gcccccagcc
     4501 cgatcgggag gagcgctgtg tgcgcgtccg gcgccgtcgc ctgcgcggcg accccttccg
     4561 ccaccgcccg cagcccgccg gtgggcaacg tcccgcttgc ctcgcggacc gtcgggaaga
     4621 acgcaacgcc ctgcagggtc atgacctggg tccggggcct cgtcaggtac tcgatcaatc
     4681 gttcgcccac gtcggaggca ggcgagtttt tcggaatgcc gaggccgacg atcaccgcga
     4741 gatacgaacg tccccgcggc ccggcgggcg acgggaggac gacgaagtcg ttgggccgct
     4801 cgcgcaacgc gggcaggagg cgggccacat gatcccaggc cacccacacc tcgcccagca
     4861 gcagcggctc ggacatgaat tcgtacacga acgaagacgg gtgcgtcagc ccccacaggc
     4921 gccgcaggta ctgccacatc gcgaccgcgt cgggcgactt gaaccgcttg acctgcgacc
     4981 cggtgaacga cggataggca tagccatgca ggaagcgccc gtagaggccg gccggtcccg
     5041 ccgggaagcc gaggcggcgc tggccggtcg cctgcttgat gttctcgccc cactgcagca
     5101 aatcgtcgta cgtcatggcc tgcggattgc ggccccgggg gaagtatggc atcgcctgtt
     5161 tgtttgcgac gatcaggtac gtggcctgca tccaggggac gaaggcctga atcccgccga
     5221 tctgtccggc cctcacgaga tccgcggcga acgaccggtc cttacggcct tccaactggc
     5281 ggagcaacgg cgcgacgtcg cgcacggcgc cctccgccac cagcagcggg aagtcgccct
     5341 gcaacgtccc ggtcaccgcc acggacccac ggcccgcctt cgcttccgca agcagccgat
     5401 cgatgtatgg acccgcggtg tcggagacaa aatcgatacg gatcccggcc tcgtcctcga
     5461 accccttcaa gagcacggtg cgggcccagt cttgttcctg caccgggttg agctgcgtcg
     5521 aggtgaacgt gatctgttgc accggagcgg gcgagggggg tacgccggca ccgagcacga
     5581 gcagggtggt gagcagtccg gcagcggtcc atcgtcgcat cgaagggtcc cccttgagcg
     5641 ggtcgaacga gtgcgccgag atttctctcc gaggttccgg aatacctcca gtggagggct
     5701 cccgttccct gacgcccgtg tcagacctag tccgaatgct cgggttaaat aggtgctcta
     5761 tcgattgtgc gcgcagtcgc tacgatccct tggttaccgc ccttcacgcg gcgtagtaac
     5821 ggaggtccac gtaggcgagc gggttgtccg gcaccctgtg cgccgcgtac tggttgcgga
     5881 gcgcgaggac catgcggagt ccctcgaagt cgggcgccgc cgccggcgca aacccgttga
     5941 acggatcgac gaggatctcg aaggcctgcg gggcgagctc cggcggaaag ttggcgttgc
     6001 gggcgaggat ctggatcccc tcggcgcgat tctcgggggt gtacaaccac agaagcgcgt
     6061 cgcggtaagc tcggatgaag cgcaccagtt ggtcccggtg cgacgctgcc caggaccggc
     6121 gcgcggcgcc caccaccccc tggtaccggt ggagaacgtc gatcgcgttg cctagcagcc
     6181 gcaacccccg ttggcgggcg agcacctcaa agggggtgac gagcagcgtg cccgcatgcg
     6241 tgcccgcgac gagggcctgc caccgttgga ggacgccccc ggccgggacg agggcgtagt
     6301 cgttgggccc gagcccgttg atctccagca tcttgcgcag gacaaacgcg tacccggtcg
     6361 agaccgcgtc caccgaaagc gcctgtccgc gcaggtcggc gtaggtctga atctcgggct
     6421 tgacgatcag ccggagaaag ccgttgtccc cgcccatgaa ggcaaacaga tccggtcggc
     6481 ccggcaccgc cgcctcaccc tcgcccgcgt cgtaggcgat cacgttgtcg atcgccgtca
     6541 tggcgatgtc gaactccccg gtgatcaggt gggtgagttg gaagacggaa cttggggtgg
     6601 gcgtcagttg cacggtgagg ccgcgggcgg cgaacaggcc tttctcctgg gccacccaca
     6661 gcgggaggtt gaacccgccg gggaaggcga tgacccggag gatctcggag gccgtggcgg
     6721 cctccgcgcg tgtcagggtc gacgccccca ggattgtgca cgccgcggtc gtgaggagct
     6781 ctcgtcgagt caacccgctc atgtcctccg tcacctgccg ggaatcgcgc cggggcgccc
     6841 gccctcgggg cggtccacta gccgtaccca gcgaccacgg cccgcgcggc gtggagcgcc
     6901 gccacgacga ggttcatccc gcggagtgtg gtctggaccg gaatccacga gttcaagatt
     6961 ctgtgtgtca cgcatacacc ctgccccccc tcgcccgcaa atgttccggt cccttgg
//
LOCUS       DASQGH010000561         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42093, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000561 DASQGH010000000
VERSION     DASQGH010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42093"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VEW91_11070"
     CDS             complement(<1..285)
                     /locus_tag="VEW91_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM92164.1"
                     /translation="MTHEGTIVHGEMLQKRFGGITAVDAVDISVAEGDLIGLIGPNGS
                     GKTTLINLLTGHLLPDAGGVFLRGRRVNGLAAHGFAALGVARTFQLSQLFG"
     gene            complement(282..1256)
                     /locus_tag="VEW91_11075"
     CDS             complement(282..1256)
                     /locus_tag="VEW91_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92165.1"
                     /translation="MRRLPVYVLVAAVVLALVYVRLLNSYWMHVAIIAMFYAILTASW
                     SLLAGYAGLFSLGHMAFASVGGYASALLVLWTGIPVPLGMAAGVALCCAVGGIIGWVC
                     LRMSGPYLAIFTLAFSEIVRITFSTEDQLTAGMGGLHTTPLLESASRLPYVYTAFGLL
                     AASLAVMGIMVHSRWGLFFRAIREDEQAAAACGVRVAHFRILAFAVASGFAGLAGGFY
                     AHYIGVLTPSLGSLDLMAIVVVMTVLGGMESLPTAVGGAITVEVLLEYLRAYGQWRLV
                     LVGILLLVTMRFARNGLLTPVWQQFTRLGEVRAAPPARRGVGQEGSRV"
     gene            complement(1260..>2106)
                     /locus_tag="VEW91_11080"
     CDS             complement(1260..>2106)
                     /locus_tag="VEW91_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92166.1"
                     /translation="ALIAVGVTLIFSILKIVNFAHGDLYMIGGYCAYYVITLLGVPPV
                     PALAAAMVSVFTFGIVLERLLLTPLYSERTERKDEYGLIVTFGLAFFLRNMALNVFGP
                     FPLRPPSFVPGLLRVGNLILTNDRVFAAAVGLLLIGLLLYFMSGTIWGQALDAVSQSR
                     ESAAIVGINPQRFNSLAFGVGAGLAAGAGALIAPIFSLAPDMGVSSNIQAYVIVILGG
                     MGSVLGSIIAGFFLGESENLFTAFFPDPTRALAYTNAFGVLVMMIVLIFRPTGLFGRR
                     HVRME"
BASE COUNT          437 a          734 c          676 g          259 t
ORIGIN      
        1 gccgaacaac tgggacaact gaaatgtccg tgccaccccg agcgccgcaa agccatgcgc
       61 ggccagccca ttcacccgcc gcccgcgcag gaagacccct cccgcatccg gcaggaggtg
      121 ccccgtcagc agattgatga gcgtcgtctt gcccgagccg ttcggtccga tcaggccgat
      181 caggtcgcct tcggccaccg agatgtcgac cgcgtccacc gccgtaatgc cgccgaaacg
      241 cttctgcagc atctccccgt gcacgatcgt cccctcgtgc gtcatacgcg ggatccttcc
      301 tgcccaacgc cacggcgggc cggaggcgcg gcgcgcacct cgcccaggcg ggtgaactgc
      361 tgccagaccg gggtcagcag gccgttacgc gcaaaccgca tcgtcaccag caacaggatc
      421 cccaccagga ccaggcgcca ctgcccgtag gctcggaggt actccagcag cacctccacc
      481 gtgatcgcgc ccccgacggc cgtgggcagg ctttccattc ccccgagcac ggtcatcacg
      541 accacgatgg ccatgagatc caaggagccg aggctggggg tgaggacgcc gatgtagtgc
      601 gcatagaacc ccccggcgag cccggcgaac ccgctggcca ccgcaaacgc gaggatgcga
      661 aagtgggcca cgcggacccc gcaggccgcc gccgcctgct cgtcctcgcg gatcgcgcgg
      721 aagaagagcc cccagcgcga gtgcaccatg atccccatga cggccagcga ggcggccagg
      781 agtccgaatg cggtatagac gtacggcagc cgggacgcgc tctccaacaa cggggtggtg
      841 tgcagccccc ccatcccggc agtcagctgg tcctcggtcg agaacgtgat ccgcacgatc
      901 tccgagaagg ccagcgtgaa gatcgcgaga tacggcccgc tcatccgcag gcagacccac
      961 ccgatgatgc ctcccacggc gcagcacaac gccacccccg ccgccatgcc gagggggacc
     1021 ggaatcccgg tccacaggac gagcagggcc gatgcgtagc cccccaccga ggcgaacgcc
     1081 atgtggccga gcgaaaacag ccctgcgtat ccggccagga gcgaccagct cgccgtcagg
     1141 atcgcataga acatcgcgat gatcgccacg tgcatccagt acgagttgag caggcgcacg
     1201 tacacgagcg ccagcaccac cgcggccacg aggacataga cggggagccg gcgcaccgcc
     1261 tactccatcc gcacgtggcg ccggccgaag agtcccgtgg gccggaagat gagcacgatc
     1321 atcatcacca gcaccccgaa cgcgttcgtg taggccagcg cgcgggtcgg atcggggaag
     1381 aaggccgtga agaggttctc tgattcaccg aggaagaacc cggcaatgat gcttcccagc
     1441 accgatccca tgccgcccag gatcacgatc acgtaggcct ggatgttgga cgacacgccc
     1501 atgtcgggcg cgagcgagaa gatcggcgcg atcagagccc ccgcgcccgc ggccaggccg
     1561 gcgccgaccc cgaacgcgag cgagttgaag cgctgggggt tgatgcccac gatcgcggcg
     1621 gactcccgcg actggctgac cgcatccagc gcctgtcccc agatcgtccc gctcatgaag
     1681 tagagcagga ggccaatcag caggaggccg acggcggccg caaacacgcg gtcgttcgtc
     1741 aagatcagat tgccgactcg caggaggcct gggacgaacg acggggggcg caacgggaac
     1801 ggcccaaaca cattgagggc catgttgcgc aggaagaacg caaggccgaa cgtgacgatg
     1861 agcccgtact cgtccttgcg ttcggttcgc tcgctgtaca ggggggtgag cagaagacgc
     1921 tccaggacga tcccgaatgt gaagacggaa accatcgccg ccgcgagcgc gggaaccgga
     1981 gggacgccga gcagcgtgat gacgtagtac gcgcagtacc cgccgatcat gtagaggtcg
     2041 ccgtgggcga aattgacgat cttcaggatc gaaaagatga gcgtcacgcc caccgcgatc
     2101 aacgcg
//
LOCUS       DASQGH010000562         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16595, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000562 DASQGH010000000
VERSION     DASQGH010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16595"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..945)
                     /locus_tag="VEW91_11085"
     CDS             complement(202..945)
                     /locus_tag="VEW91_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92167.1"
                     /translation="MTAPLLQVSGLVARRGVVEVLHGIDLEVRPGEVLAIIGPNGAGK
                     STLLGTIAGVYPARAGTITFAGQPITGLPAEAALRLGISLVPERRQVFNLLTVRENLL
                     LGGYHRYWRHRAEVRADVALPQGIFPKLLGLDRRLGGSLSGGEQQMLAIGRGLMSRPR
                     ILLLDEPSLGLGPLIVREIVHVLERLRRAEGLTVVLVEQNVKAAMAIADRACVMDRGR
                     IILRGTPNELLAHPGIKAAYLGKGYEIAG"
     gene            complement(942..1784)
                     /locus_tag="VEW91_11090"
     CDS             complement(942..1784)
                     /locus_tag="VEW91_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92168.1"
                     /translation="MTAPLLEVVDLSKRFGGVSAVHRVHFAAAPQEIAAVIGPNGAGK
                     TTLFNLMTGFVPPTSGDVFYRGQRITGMPAPRIAWLGIIRTFQNPMIFNETTVVENVM
                     VGCHRWTRTGFVQAALGLPAAVREDRTVYATAREALGLVGLDGRAHDLARDLPFGQQR
                     QLEIARALVARPSLLLLDEPTAGLSPQEARSLVGLVRRLRADGVTIVVIEHNMDVVME
                     AADRVIVLDYGEKIAEGPPQAIQRDPRVIAAYLGEEPGVPGDAAQDAGAARGQRAIPE
                     RPGP"
     gene            complement(1781..2794)
                     /locus_tag="VEW91_11095"
     CDS             complement(1781..2794)
                     /locus_tag="VEW91_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92169.1"
                     /translation="MREGAGLALLACGIAVLPFLASTAYALTVLNIIGLYTIVTLGLI
                     LLGSSGQVSLGQAAFYGLGAYASALLSRDLGWSPWVTMPVAIIATAGVAYVVGLPTLR
                     LAETFFVLATLGFGIIVNVLMVQLVPLTGGATGLRDIPGLQIAGVALATDRAFYYLIW
                     GLALAALLLARNITSHRTGRALRAILGSEVGAAARGVEVSRYKMQVFVASAAYAALAG
                     GLYAHYIRFISPSPFSLYASLFFLIMAAVGGLTSIWGGLLGAAVITLLDQVLRAEVPR
                     VFPRVGGEYQTAIYGILLVLIMIFFPSGIVRGALDLRRLFRQAAINRPGAARPEPAKG
                     GGA"
     gene            complement(2794..3675)
                     /locus_tag="VEW91_11100"
     CDS             complement(2794..3675)
                     /locus_tag="VEW91_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92170.1"
                     /translation="MIVLALLPQLLLTGITVGSIYALVALGFVLIYGVTGILNFAQGE
                     FSMLGALFCATLLAWHLPLGTAALIAVAAVCVLGALTERLAVEPAFGAHPLTLIIITF
                     GVSMAFRGLALMLWGADPYALPEFTPGPALVVLGGVLIRQAMWAIGLSVAVVGALFVF
                     FARTVPGAAVRACADNAFACRLVGISPRRMSLVAFTLSAGLGAVAGIVITPITGATYD
                     MGLALGLKGFVAAVIGGLSNAPAAVVGGYLIGIIESLTTGYLAPGYAGAITFLVLLVV
                     LFLQREGLLHGAQRRQA"
     gene            complement(3715..4884)
                     /locus_tag="VEW91_11105"
     CDS             complement(3715..4884)
                     /locus_tag="VEW91_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92171.1"
                     /translation="MTVRPLSQRLIILGLTTALAAGLLVPARPVHGQQAPIKIGTVFA
                     VTGPGSSLGRPEKDTATMLQTQLQSAGGIGGRPVQIITYDSESDPTKAVLLIKKAVTE
                     DNVVAVVGGTTSPESQAMADYAMSADIPFMSVAASTTLSVPSRAWVFQMPQRNATAAA
                     KALEYLVAIHAKTFAFLYRNDDFGQDGLVALRTYGLHQGVTLVDTEPFAATDTDLSVQ
                     VARARVKNPDAMVVWSTPPTASIAAKNIRQLGMNTPIVESHGIANKAFIQLAGPAAEG
                     VVFPAGKLLVADRLPASDPQKALLEKYAKDFEAANKYAASTFGGHAYDGITMLADALR
                     HVGPDKAKIREYLEHLRGFLGTGGVFNMSPQEHNGLSTKDMVLVVIKNGNWDIWK"
     gene            5096..>5761
                     /locus_tag="VEW91_11110"
     CDS             5096..>5761
                     /locus_tag="VEW91_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HYM92172.1"
                     /translation="MPARTGAQFLQGLRDRPRDLWIEGQRVADPVEHPAFRNVVRSVA
                     ALYDMQHAPALRDEMTYVSPATGDRVGLSFLTPRTPDDLVRARKMMKRWADYSGGFMG
                     RTPDYLNRALVGFASAAEYCAENDARFGDNIRRYYEHVREHDLCLTHTLVNPQANRAV
                     GPAQQADPYLAARIAEETKSGIVLRGARMLATLPVSDEIMVFPSTLLKAGAEDAPYAY
                     ALAI"
BASE COUNT          928 a         2029 c         1986 g          818 t
ORIGIN      
        1 cccacccacc ccgcttccca tttccggcgg tagaagggat tggtggcccg gatccgggag
       61 agcatcggag cgagccgctg ccactggtag gcggcgagat cctcccgccc catggtttcc
      121 agcgtcggat ccacaaacag cgcgggatcg tcgacgcgca gcgtcacgac acgtgctcct
      181 cggtgaggat ccggcgcaca ctcatccggc gatctcgtac cccttgccca ggtacgcggc
      241 cttgatcccg gggtgggcga gcagctcgtt cggggtgccc cggaggatga tgcggccccg
      301 gtccatgacg cacgcgcgat ccgcgatcgc catcgctgct ttgacgttct gctcgacgag
      361 gaccacggtc aacccttcgg cgcggcgcag gcgctcgagg acgtggacga tctcccgaac
      421 aatcaggggc ccgagtccga gcgagggctc gtccagcagc aggatgcggg ggcgggacat
      481 cagcccgcgc ccgatcgcga gcatctgctg ctcccctccg ctgagggagc cccccaaccg
      541 tcggtcgagg cccagcagtt tcgggaagat gccctgcggc agcgcgacgt cggcgcgcac
      601 ctccgcgcgg tgccgccagt accggtggta tcccccgagc agaaggttct cgcgcaccgt
      661 cagcaggttg aagacctggc ggcgctcggg cacgaggctg atccccagcc ggagggccgc
      721 ctccgcgggc agcccagtga tcggctggcc ggcgaaggtg atcgtcccgg cccgggccgg
      781 atacacgccg gcgatggtgc cgaggagcgt ggacttcccc gcgccgttcg gcccgatgat
      841 cgccaggacc tcccccgggc ggacctccag atcgatccca tggaggacct cgacgacacc
      901 tcttcgcgca acgaggcccg agacctggag caggggagcg gtcacggccc cggcctctcc
      961 gggatcgcgc gctgcccgcg cgctgccccc gcgtcttggg cggcgtcccc cgggacgccg
     1021 ggttcctcgc ccagataggc ggcgatcacc ctcgggtcgc gctggatcgc ctggggcggg
     1081 ccttcggcga tcttctcgcc gtagtccagc acaatcaccc ggtccgccgc ctccatcacg
     1141 acgtccatgt tgtgctcgat gaccacgatg gtgacgccgt ctgcccggag gcgccggacc
     1201 aggccgacga ggctccgggc ttcctgcggg ctgaggcccg cggtcggctc atcgagcagg
     1261 agcagcgatg gacgggcgac cagggcgcgg gcgatctcca gttgccgctg ctggccgaac
     1321 gggagatcgc gcgcgaggtc gtgggcgcgc ccgtcgaggc cgacaaggcc gagcgcctcc
     1381 ctggccgtcg cgtagaccgt ccgatcttcg cgcaccgcgg ccgggaggcc cagcgcggcc
     1441 tggacgaacc cggtgcgggt ccaccggtgg caccccacca tcacgttctc cacgacggtc
     1501 gtctcgttga agatcatcgg attctgaaac gtcctgatga tccccagcca cgcaatgcgg
     1561 ggagccggca tcccggtgat ccgttgaccg cgatagaaga cgtcgccgga cgtggggggg
     1621 acgaaccccg tcatgaggtt gaacagcgtc gtcttccccg ccccgttggg cccgatcacg
     1681 gcggcgatct cctgcggtgc ggcggcgaag tggaccctgt ggaccgcgct aacgccaccg
     1741 aaccgcttgc tcagatccac cacctcgagc aggggcgccg tcacgcgcca ccccccttcg
     1801 cgggctcggg gcgggcagcc cccggccggt tgatcgccgc ctgccggaag aggcgcctga
     1861 ggtccagcgc cccgcggacg atccccgagg ggaagaagat catgatcagc acgagcagga
     1921 tcccatagat cgccgtctgg tactcgccgc ccacccgcgg aaagacccgg ggaacctcgg
     1981 cgcggaggac ctggtccagg agcgtgatga ccgccgcacc gagcagtccc ccccagatgc
     2041 tcgtcaaccc gccgacggcc gccatgatga ggaagaacag cgaggcgtac agcgagaacg
     2101 gcgaggggct gatgaaccgg atgtagtgcg cgtacaggcc tccggcgagc gccgcgtacg
     2161 ccgcgctcgc gacgaacacc tgcatcttgt accgcgacac ctcgacccca cgggccgcgg
     2221 ccccgacctc gcttccgagg atcgcccgaa gcgcccgccc ggtccggtgc gaagtgatgt
     2281 tccgggcaag caggagggcg gccagggcga gcccccagat caggtagtag aacgcccggt
     2341 cggtcgcgag cgccacccct gcgatctgga ggccggggat gtcgcggagc ccggtggcgc
     2401 ctcctgtcag ggggacgagc tgcaccatga ggacgttgac gatgatgccg aacccgagcg
     2461 tggccagcac gaagaaggtc tcggccagcc tcagcgtcgg cagccccacg acgtaggcca
     2521 ccccggcggt ggcgatgatt gcgaccggca tcgtcaccca cgggctccac cccaggtccc
     2581 ggctcagcag cgcggacgcg tacgccccca gcccgtagaa cgccgcctgc ccaagggaga
     2641 cctggccgga ggaccccagg aggatcaggc ccagggtgac gatggtgtag agtccaatga
     2701 tgttcaggac ggtgagggca taggccgtcg aggcgaggaa cggcagcacc gcgatcccgc
     2761 aggccagcag cgcgagtccg gctccctcgc gcatcacgcc tgcctccgct gggccccgtg
     2821 cagcagtccc tcgcgctgca agaacaggac gacgaggagc acgaggaacg tgatcgcgcc
     2881 ggcgtacccg ggcgcgagat acccggtcgt gagcgactcg atgatcccga tgaggtatcc
     2941 gcccacgacg gcggccgggg cgttgctgag cccgccgatg accgcggcga cgaacccctt
     3001 caacccgagg gcgagcccca tatcgtacgt cgccccggtg atcggggtga tgacgatgcc
     3061 cgccacggcg cccagtccgg cgctcagggt gaacgccacc agcgacatcc gtctggggct
     3121 gatgccgacg aggcggcacg cgaacgcgtt atccgcgcac gcgcgcaccg ccgctcccgg
     3181 aaccgtccgg gcaaagaaga caaagagcgc gccgacgacc gccaccgaga ggccgatcgc
     3241 ccacatcgct tggcggatga gcacgccgcc gagcaccacc agggcgggcc ccggggtgaa
     3301 ctccgggagc gcgtacgggt ccgcccccca gagcatcaag gcgaggccgc ggaaggccat
     3361 cgagacgcca aaggtgatga tgatcagggt cagcgggtgc gcgccgaacg cgggctccac
     3421 cgccagccgt tcggtcagcg cgccgagcac gcacaccgcc gccaccgcga tcagcgcggc
     3481 cgtcccgagc ggaaggtgcc aggccagcag cgtcgcgcaa aagagcgccc ccagcatcga
     3541 aaactcccct tgggcgaagt tcaggatgcc ggtcacgccg tagatgagga cgaacccgag
     3601 ggcgaccagc gcgtagatgc tgccgaccgt gatcccggtg agcagcagtt ggggcagcag
     3661 ggcgagcacg atcagaggag cgggccggcg gccggccggc agctccgtgc cccgctactt
     3721 ccagatatcc cagttgccgt tcttgatcac gacgaggacc atatccttgg tgctgagccc
     3781 gttgtgttcc tggggagaca tgttgaagac cccgccggtc ccgaggaacc ctctgaggtg
     3841 ctccaggtac tcgcggatct tcgccttgtc ggggccgacg tgccggagcg cgtccgcgag
     3901 catcgtgatg ccgtcgtacg cgtggccgcc gaaggtgctg gcggcgtact tgttggcggc
     3961 ctcaaagtcc ttggcgtact tctccaagag cgccttctgc gggtcgctgg ccggcagcct
     4021 gtcggcaacg agcagcttcc cggcggggaa caccacgccc tccgcggccg gcccggccag
     4081 ctggatgaac gccttgttgg cgatcccgtg gctttccacg atcggggtgt tcatcccgag
     4141 ctggcggatg ttcttcgccg cgatcgacgc ggtgggcggg gtgctccaga cgaccatcgc
     4201 gtccggattc ttgaccctgg cgcgggccac ctgaacgctc agatcggtgt cggtggctgc
     4261 gaacggctcg gtgtcgacga gcgtgacccc ctggtggaga ccgtaggttc ggagcgcgac
     4321 caggccgtcc tggccgaagt cgtcgttccg gtagaggaac gcaaacgtct tcgcatggat
     4381 ggccaccagg tactccaacg ccttggccgc cgccgtggca ttccgctgcg gcatctggaa
     4441 cacccaagcg cggctgggga cgctgagcgt cgtgctggcc gccaccgaca tgaacgggat
     4501 gtccgcggac atcgcgtagt cggccatcgc ctgcgactcc gggctggtcg tgccgccgac
     4561 gaccgccacc acgttgtcct cggtgacggc cttcttgatc agcaggaccg ccttcgtggg
     4621 atcgctctcg ctgtcgtagg tgatgatctg aaccggccgg ccgccgatcc cgcccgcgct
     4681 ctggagctgg gtttggagca tcgtcgccgt atccttctcc ggtctgccga gcgagctgcc
     4741 cggccccgtg acggcgaaga ccgtcccgat cttgatcggt gcctgctggc cgtgcacagg
     4801 gcgcgcgggc acgagaagcc ccgcggcaag tgctgtggtg aggccgagga tgatgagcct
     4861 ctgcgatagc ggtcgcactg tcatgacccc cctctggagc gtccccctcg caatgggcgt
     4921 gggaggccgc cgggacggct ctgcgcgccc tcccggcgga ttgcccgatt gccacggtct
     4981 tatacggatg ctgggggaaa ccctctctcc gggtgctgcg gccccggcag gagcgtccgg
     5041 aggccggagc gaacctgcgc cctgcctagc ctggccgttc gagggggacc cacccatgcc
     5101 agcgagaacc ggagcgcagt tcctccaggg gctgcgggat cggccgcggg acctgtggat
     5161 cgagggccag cgggtcgcgg acccggtgga gcatcctgcg ttccgcaacg tggtccgcag
     5221 cgtcgcggcg ctctacgaca tgcagcacgc ccctgccctc cgcgacgaaa tgacctacgt
     5281 ctcgccggca acgggcgacc gcgtgggcct ctcattcctc acgccccgca cgcccgacga
     5341 tctggtccgc gcacggaaga tgatgaaacg gtgggcggac tactcggggg ggttcatggg
     5401 gcgcacgccc gactacctga accgcgccct cgtggggttc gcttccgccg cggagtactg
     5461 cgctgagaac gatgcgcgat ttggggacaa catccgccgg tactacgagc acgtccgcga
     5521 gcacgacctg tgcctcaccc acaccctggt caatccccag gcgaaccgag cggtggggcc
     5581 ggcgcagcag gccgacccgt atctggcggc gcgcatcgcg gaggagacaa agagcggaat
     5641 cgtcctccgg ggggcgcgca tgctggccac cctgccggtg tccgacgaga tcatggtgtt
     5701 tccctcgacc ctcctcaagg ccggcgcaga ggacgctccc tatgcgtacg ccctggcgat
     5761 t
//
LOCUS       DASQGH010000563         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42095, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000563 DASQGH010000000
VERSION     DASQGH010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42095"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VEW91_11115"
     CDS             <1..190
                     /locus_tag="VEW91_11115"
                     /inference="COORDINATES: protein motif:HMM:NF023785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYM92173.1"
                     /translation="REAAWRRAHEWGEKIPIGIFYQVTGEPTYEDQVSALRAGPVAKR
                     TLQPLTRAQADALRKEFF"
     gene            202..705
                     /locus_tag="VEW91_11120"
     CDS             202..705
                     /locus_tag="VEW91_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYM92174.1"
                     /translation="MPATSHQIEERAGGRRPVVGTADERLSVLQREGYRATPQRRAIL
                     VAMLRAGRENAGAEEICRRARAVCPTVNLATTYRTLDLFGRLGMVRRLSYGDGRSVFC
                     PNARPHYHGTCLRCGAVVDIPRGRVNKALEQEQSKLTDRAFIVVGHRIEFYGYCAGCR
                     GPASAAS"
     gene            714..1577
                     /locus_tag="VEW91_11125"
     CDS             714..1577
                     /locus_tag="VEW91_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HYM92175.1"
                     /translation="MRARTAALVVVAGLGWGIGFAGPSAIPSSAAARPALPGPVTLNA
                     DSITVDNRGTTLVARGHVAATYGALRVTSDALRINRPTGAATFRGRVAVTDPKGRASA
                     QEVTLTMTGGNRVTLVMLSGRASVETPSYALLADLIAADRQHDRLEASGNVTLFSQPD
                     LIVTGTRLSYDEGTRYAVIRGDAATRATIQNRDGRIRGRWMEVFRKTGQAIIHGPVDA
                     EIYDATLTGSDATIDLDRGIAVVMGRVAVFRRQGTLLADRVTVYYRARRFVAEGTTHL
                     TLRDLDESSSP"
     gene            1578..1937
                     /locus_tag="VEW91_11130"
     CDS             1578..1937
                     /locus_tag="VEW91_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92176.1"
                     /translation="MPPLLRWLAAVAAGVAVFEALDAVAVRGPAVGWLALVSPLIAGA
                     VAAWAMGGGAGGQLLASAAVAWARIGTDRAVGILHGVRQPPEMEVAIALIFGLPWMGL
                     AVGGGALQMLARRAARR"
     gene            complement(1951..>2767)
                     /locus_tag="VEW91_11135"
     CDS             complement(1951..>2767)
                     /locus_tag="VEW91_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92177.1"
                     /translation="RRAHSSRPPAGLPLGRRPGGPPVGIHPTGVGEWSPLVEFSSPGG
                     LNVSREVQRIMRIAAGIVAAAVALGALSVAPARAQRAGESWGPLIPGLKAQLAAKPDP
                     ELALRLAMVYAHDGLMIEWWHTLKQIDRMTGGEGRRQEVSRQVIPAASGDVKRDPGNL
                     LARYELAFASWFVEPDHHTALGEFLEITRREPRNAMNHGYLGYIYADRQDAKNTIAQW
                     EDGVRLDPGNSAIRYLLGTAYSRVGRTADAAVQFALAYRDRTVYNYVTRGELP"
BASE COUNT          385 a          958 c         1009 g          415 t
ORIGIN      
        1 ccgcgaggcg gcatggcgcc gggcgcacga gtggggtgag aagatcccga tcgggatctt
       61 ctaccaggtg accggcgagc cgacctatga ggatcaggtc tctgcgcttc gggccggccc
      121 cgtcgccaag cggacgctcc agccgctcac ccgggcacag gcggacgcgc tgcgcaagga
      181 gttcttctga ccggaggagc tgtgcccgcg acgtcgcacc aaatcgagga aagagccggc
      241 ggtcgccgcc ccgtcgtcgg cacggccgac gagcggctga gcgtgctcca gcgggaaggg
      301 taccgcgcca cgccccagcg ccgcgcgata ttggtcgcca tgctccgcgc cggccgcgag
      361 aatgccggcg cggaggagat ctgccggcgc gcgcgggcgg tgtgccccac ggtcaacctc
      421 gccaccacgt accgcaccct ggacctcttc gggagactgg ggatggtccg gcgcctctcg
      481 tacggcgacg gccggagcgt gttctgcccg aacgcccgcc cccactacca tgggacctgc
      541 ctgcggtgcg gggccgtcgt cgacatcccc cggggacggg tcaacaaggc gctcgagcaa
      601 gagcagtcca agctgaccga tagggcattc atcgtggttg gccaccggat cgagttctac
      661 gggtattgcg ccgggtgccg gggccccgca tccgcggcca gctagtcgcc tcgatgcgcg
      721 cgcgcacggc cgccctggtc gtggtggcgg ggctgggatg ggggatcggc ttcgccgggc
      781 cgtccgcgat cccctcgtcc gccgcagcga ggccggcgct ccccgggccg gtgacgttga
      841 acgccgacag catcaccgtc gacaaccggg gaaccaccct ggtggccagg gggcacgttg
      901 cggcaacgta cggggcgctg cgcgtcacca gtgacgcctt gcggatcaac cgaccgaccg
      961 gcgccgcaac gtttaggggc cgcgtggcgg tcacggatcc aaaggggcgg gcctccgctc
     1021 aggaagtcac gctcaccatg acgggcggaa accgggtcac cctggtgatg ctgagcggtc
     1081 gcgcctcggt cgagacccca tcctatgccc tcctggcgga cctgatcgcc gccgatcgcc
     1141 agcatgaccg cctggaggcc tccgggaacg tgacgctctt ctcccaaccg gacctgatcg
     1201 tgaccggcac acgcctttcc tatgacgagg gcacgcggta cgcggtgatc cgcggggacg
     1261 ccgctacccg ggcaacgatc cagaaccgcg acggccgcat ccgcgggagg tggatggagg
     1321 tgttccgcaa gaccgggcag gcgatcatcc acgggcccgt cgatgcggag atctacgacg
     1381 ccaccctcac cgggtccgat gcgacgatcg acctcgaccg ggggattgcc gtcgtcatgg
     1441 gccgggtcgc ggtctttcgc cggcagggga cgctcctggc cgaccgggtg acggtctact
     1501 accgggcccg gaggttcgtc gccgagggca ccacccacct gaccctcagg gacctcgacg
     1561 agtcttcatc tccgtagatg cccccacttc tccgatggct cgccgccgtc gctgcgggcg
     1621 tggccgtctt tgaggcgctc gacgcggttg ccgtcagggg gccggcggtg gggtggcttg
     1681 cgctggtgtc cccgctcatc gcgggggcgg tcgcggcgtg ggcgatgggc ggcggggcgg
     1741 gcggccagct gctggcgtct gcggccgtgg cctgggcccg gatcggcacc gatcgggccg
     1801 ttggcatcct ccacggcgtc cgccagccgc ccgagatgga ggtggccatc gcgttgatct
     1861 tcggcctgcc gtggatgggg ctggcggtgg gaggcggggc gctccagatg ctcgcccgcc
     1921 gggccgcgcg gcggtaagtc tggcggcacg ctacggcagt tcgccgcgcg tcacgtagtt
     1981 gtagacggtg cggtcgcggt aggccagggc gaattggacc gcggcgtccg cggtgcggcc
     2041 gaccctgctg tacgcggttc ccagcaggta gcggatcgcg ctgttgccgg ggtccagcct
     2101 gacgccgtct tcccactgcg caatggtatt cttggcgtcc tgccggtccg cgtagatgta
     2161 cccgaggtac ccgtggttca tggcgttgcg gggctcccgc cgcgtgatct caaggaactc
     2221 cccgagcgct gtgtggtggt ccggctccac gaaccacgac gcgaatgcca gctcgtagcg
     2281 ggccagcagg ttgcccggat ctcgcttgac gtcccccgag gcagccggga tgacttggcg
     2341 gctgacctcc tgccggcggc cttcaccccc ggtcatccgg tcgatctgtt tgagcgtatg
     2401 ccaccactcg atcatgagcc catcgtgcgc gtacaccatc gcgagccgca gcgcaagttc
     2461 ggggtcgggt ttggcggcga gttgcgcctt gagccctggg atgagcggcc cccagctctc
     2521 cccggcccgc tgggcgcggg cgggcgcgac ggatagggcg ccgagggcta cggcggcggc
     2581 gacgattcca gccgcgatgc gcatgatccg ctggacctcc cgggagacgt tgagcccacc
     2641 tggggaactg aattcgacaa gcggggacca ttcccccacg cccgtagggt gaatccccac
     2701 gggagggccc ccgggccggc gccccaaagg gagccccgcc ggggggcgag aactgtgcgc
     2761 acggcga
//
LOCUS       DASQGH010000564         1523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30907, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000564 DASQGH010000000
VERSION     DASQGH010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30907"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEW91_11140"
     CDS             complement(<1..209)
                     /locus_tag="VEW91_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92178.1"
                     /translation="MGIRIHPVDERKVDERVRQAFADAASRGAPNPTLLRILARHPVA
                     LQAFYAGWQDTFYGGIVEHPLKELMR"
     gene            complement(345..875)
                     /locus_tag="VEW91_11145"
     CDS             complement(345..875)
                     /locus_tag="VEW91_11145"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HYM92179.1"
                     /translation="MLQKRVHRNYRIRDTGTGLLEAVLGICAAAHGPDEVVQGEVVRV
                     RAHLDSSTYRPYQQLRLAVELAVREGWHIYTAPVPAGYIPLRVEITPFDGLESGQPVW
                     PPGRRFTVAGLDEEFFVLDGTVRASIPLTLSVAPGRGDLRIAVAAHYQACSETACLPP
                     TVARVDVSVSEAPFPE"
     gene            complement(858..1157)
                     /locus_tag="VEW91_11150"
                     /pseudo
     CDS             complement(858..1157)
                     /locus_tag="VEW91_11150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(1170..1322)
                     /locus_tag="VEW91_11155"
     CDS             complement(1170..1322)
                     /locus_tag="VEW91_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92180.1"
                     /translation="MAEDLSQQGPKIGVRFPDVALRDQRGTLVDLHQARRGHQALIVF
                     YRSARW"
BASE COUNT          289 a          500 c          479 g          255 t
ORIGIN      
        1 cgcatcaact ccttcagcgg atgctcgacg atgcctccat agaacgtgtc ctgccagccc
       61 gcgtagaagg cctgcaacgc cacaggatgg cgcgcgagaa tccgcagcag cgtggggttg
      121 ggcgcgcctc gggaagcggc atcggcaaaa gcctggcgga cccgctcgtc aaccttccgt
      181 tcatcgacgg gatggattct gatccccata tgcactcccc ctcatgcttc ctcaatctcc
      241 ccttggttgg ttgacgcgta gggtgcgaca cccaagcggt cgggcggcat ggggggtgtc
      301 ttgcggcacg ccagcggggg agccgcagcg tgtcgcgatc ttggtcattc cgggaaaggc
      361 gcttcgctga cagacacgtc cacgcgcgcg accgtgggcg gtagacaggc cgtctcgctg
      421 catgcctggt agtgtgccgc cacagcgatc ctcaggtcgc ctcgccccgg tgcgaccgac
      481 agcgtcaacg ggatactcgc acggaccgtc ccatcgagaa cgaagaactc ctcatcgagt
      541 ccggccactg tgaaccggcg gcccggcggc cagacgggtt gtccggactc aagaccatcg
      601 aacggcgtga tctccacacg gaggggaata tatcccgcgg ggaccggtgc cgtgtagata
      661 tgccagccct ctcgaacggc caattcaacc gcgagccgta gttgctggta cggccggtag
      721 gtggaggagt cgaggtgggc acggacccga acaacctcgc cctgaacgac ctcatccggc
      781 ccatgcgccg cggcacagat gccaaggacg gcctccagca atcccgttcc ggtgtccctg
      841 atgcgatagt ttcgatggac ccgcttctga agcacggtgc cgtcgcgatc gaggagaaaa
      901 atcatcggat acgcgacgcc acgctggtcg tcccgcacgc tccgcccaaa ctcggcatga
      961 tgacggtcga ggtcgtcatc gagcatgccg aggcggcgaa tctcacgact cccgtcgtct
     1021 gagagcaggg agtaggttat cccgtatttc gcggcgaacg cagcgagcgt gtccacggag
     1081 tcatagctga tagcaaacag ccccacaccg gccgcttgaa gatcccccgc aatccgttgc
     1141 agctcgacga gctgcgtctt gcagtaaggt caccaccgag cgctgcgata gaaaacgatg
     1201 agcgcctggt gcccccggcg ggcctggtgg agatcgacga gcgtgcctcg ctggtcgcgc
     1261 aaagcgacat ccggaaaccg caccccgatc ttgggaccct gctgactcag atcttccgcc
     1321 acgtccgacc ctcctccatt ccccggacag gagaggcctg ccacggcact gcttctggtg
     1381 aacgctcatg gagcgctgat tatgccggtg aggcctaggc cgggaagagc gtcagaggag
     1441 ccgtcacaga tgtccccgcg tcgtaccagc ggcggtgccg tggacgtccg gatcacgatc
     1501 tctctccggc agcgtcaagg gtg
//
LOCUS       DASQGH010000565         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50595, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000565 DASQGH010000000
VERSION     DASQGH010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50595"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1368)
                     /locus_tag="VEW91_11160"
     CDS             complement(109..1368)
                     /locus_tag="VEW91_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92181.1"
                     /translation="MRVIGRWMLTGCVVVLLVAGLILAPIGTLRWGAQAAATPIKLGE
                     VGPLSPPGGYADGQLMKDAAILATDEINAKGGVLGRPIEVSYQDTRGLPEEGTAAAER
                     LTAQEHVAAIFGEFHSSVFLAEMEVVHKTGTPILAVDVWANKITGKGYAEVFRLAPCQ
                     ALIATQYGDWMAAAGFKNVVVLWEKTDGGQSHRDVLLGVLQKHGIKYDVVGADLDQKE
                     FTAQIERLKSHTPPYDFFATAFSEAGLYALVSQAHSLGFAPTAKTGMYNSGGPATTPT
                     FWQNVGENGKYLITEVPGLPKSGWNNKTRAFVAAFKKRFNSPPTPQAMESYDAEYVLV
                     DAIRRAGSTDPKAIIRALEKTSYVGTRGRTTFATDHNPDWHYHQFVTAPVHLIEYNQV
                     GQTPDDAPVIWPRNVATAKTVYLRPMK"
     gene            1890..>2436
                     /locus_tag="VEW91_11165"
     CDS             1890..>2436
                     /locus_tag="VEW91_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYM92182.1"
                     /translation="MSLWTVFPDDAPSKQAGTLAEQIERDGGHPLAIYREPVGDHWQI
                     FCLLPTTKVEPTPYQRDLSPAHIKRLLTAVKKIDRFIDPVVVTSPRPGVYWTPNGNHR
                     RAVLEKLKAKLIPAIAVPEPELTFQILALNTEKAHNLKEKSLEVIRMCRDLMKEDPSH
                     GEEDYAFQFEAPHFITLGLLYE"
BASE COUNT          404 a          837 c          749 g          446 t
ORIGIN      
        1 aggtaatcca tggcgttaga tgcggggggg cccgcacgcc cgccggcggt acgcgccgcg
       61 ggtgcgagcc ccccgcacga tggtgtgctt acccgagcga gcggtggatc acttcatcgg
      121 ccggaggtac acggtcttcg cggtcgcgac attcctcggc cagatcaccg gtgcgtcgtc
      181 gggcgtttga ccgacctggt tgtactcgat gaggtgcacc ggtgcggtca cgaactggtg
      241 gtagtgccag tccgggttgt gatccgtcgc gaacgtggtt ctcccgcggg tgcccacata
      301 gctcgtcttc tcgagcgcgc ggatgatcgc cttgggatcg gtgctcccgg cgcgcctgat
      361 cgcgtccacg aggacgtact cggcgtcata gctctccatc gcctgcggag tcggagggct
      421 attgaaccgc ttcttgaacg cggctacgaa cgcccgcgtc ttgttgttcc atcccgactt
      481 cggcaacccc ggaacctcgg tgatcaggta tttcccgttc tccccgacgt tctgccagaa
      541 ggttggggtg gtcgcgggac cgccggagtt gtacatgccc gttttggccg tcggcgcgaa
      601 cccgaggctg tgcgcctggc tgaccaacgc atagagcccg gcttcgctga acgcggtggc
      661 aaagaaatcg tacggcgggg tgtggctctt gagccgctcg atctgggccg tgaactcctt
      721 ctggtcgagg tcggcgccaa ccacgtcgta cttgatcccg tgcttctgca ggaccccgag
      781 gagcacgtcc cgatggctct gcccaccgtc cgtcttctcc cacagcacca cgacgttctt
      841 gaacccggcc gctgccatcc agtccccgta ctgcgtcgcg atgagcgcct ggcagggggc
      901 cagccggaac acctccgcat accccttccc cgtgatcttg ttcgcccaca cgtctacggc
      961 caggatcggg gtgccggtct tatggacgac ctccatctcg gcaaggaaca ccgagctgtg
     1021 aaactcgccg aagatggcgg ccacgtgctc ctgcgcggtc agccgctccg cggcggcggt
     1081 gccttcctca ggcaggccgc gtgtatcctg gtagctgacc tcgatgggcc ggccgagcac
     1141 cccacccttg gcgttgatct cgtccgtcgc gaggatcgcg gcgtccttca tcaactggcc
     1201 gtccgcatac cctcccggcg gcgacaacgg gccgacctca cccagcttga tgggtgtcgc
     1261 agcagcttgg gcgccccatc ggagagtgcc gattggggcg agaatcaatc ctgcaaccag
     1321 aaggacgacg acacaaccgg tcagcatcca tcgtccaata acgcgcattg ctccccctcc
     1381 tttcttgtct tggtcgtgcg ctggcacgta tcgaaaaagt ccgagaaccc tcccttgatg
     1441 agccgtcggg gccgggtgcg taagcagggg cctgtgtgcg aggaagagtg ggtgcccgtg
     1501 ctgcgaaggc tccgttctta cgcccttcct tccatcgccg cgcggggaaa tcctccccct
     1561 cctgcccgcg cgaatgccgc cggccgggtg aagagtgtgt taagttagag tgtgctagcc
     1621 ggccttcctg agccgggggt ccagaaggtc cctgactctg tcccaatccc ggcacggtgc
     1681 agcaatgggg agcgtgcgcc ccgactatag cccagaccgg caaactccta cacttcgttg
     1741 cgcgctgttc gacgcggcgg aggacgaacc ttctaggtgt gaaagagccg gggccccggg
     1801 tgaccgaact cgcgccgccc cgcaggcccg acaggacccg ggtcgctttc ccacgaagcg
     1861 ctcggctcgc actccctggg agggacgcga tgagcctctg gacggtcttc cctgacgacg
     1921 ccccctcgaa acaagccggc actctggccg agcagattga gcgtgacggc ggccaccccc
     1981 tcgccatcta ccgcgaaccc gtgggggatc actggcagat cttctgcctg ctgcccacga
     2041 caaaggtcga gccgacgccc tatcagcgcg acctctcccc ggcccacatc aagcggctgc
     2101 tgaccgcggt caagaagatc gaccgcttca tcgatcccgt cgtggtcacc tcgcccaggc
     2161 cgggcgtgta ctggaccccg aacggcaatc accgccgggc cgtcctggag aagctcaagg
     2221 ccaagctgat tccggccatt gccgtgcccg agcccgaact caccttccag attctcgcgc
     2281 tgaacaccga gaaggcccac aatctgaagg agaaatccct cgaggtcatc cgcatgtgcc
     2341 gtgatttaat gaaggaggac ccctcgcacg gcgaggagga ctacgccttc cagttcgagg
     2401 ccccgcactt catcacgctc ggcctcctct acgaga
//
LOCUS       DASQGH010000566         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13972, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000566 DASQGH010000000
VERSION     DASQGH010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13972"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..208
                     /locus_tag="VEW91_11170"
     CDS             11..208
                     /locus_tag="VEW91_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYM92183.1"
                     /translation="MAVEAGHAIMTFAFDTLGAKALFAGHHPANTASRAVLEKLGFCC
                     THEEFYPATGLNHPSYLLTRP"
     gene            complement(284..2377)
                     /locus_tag="VEW91_11175"
     CDS             complement(284..2377)
                     /locus_tag="VEW91_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYM92184.1"
                     /translation="MYRIGIDVGGTFTDLVSVDDNGRVALAKSASTPDDPSRGIMDGL
                     GLLAREVGRDLGALLAETDRLVHGTTVATNALVERKGARVGMLTTEGHRDILEMREGL
                     KEDRYNLRMPPPVPLVPRALRLGVRERVRFDGTVAAPLNRRSLDAAIRTLAREDVEAV
                     AVCYLHAYRNPRHERTTRRVVAGRLPRAYVCTSFEVLPQIKEYERICTTVVNAYVGPV
                     LSRYLGALAGRLAAAGYRREVLIMQSHGGVAPIRDSARLAAGAILSGPAGGLAGSRLC
                     GHLLDQQDLITFDMGGTSTDIALLEGGEPPLAGDRVVSGHKVALPSLDIHTVGAGGGS
                     IARVAGGLLYVGPDSAGADPGPACYGKGGTGATVTDANVVLGLLDPANFLGGRARLDP
                     EAAGRAVARVARQLGCPVVAAAEGIHRVVNTNMAEGIRRVSVRRGVDPRRFTVLAFGG
                     AAGLHVTQVARQLEITRVIVPRAAAVLSAWGMLTTDLRYELVRTHVGEIHRVGGGGVR
                     RLFAEMEAEGRTRFGQAFTGPMTMRRAVDMRYGEQIFEITVSLDGLDMAAPDLIDQIV
                     GRFQRRHEALYTYSAPEQDIVLVNARVTVVGELPVVPVESPIGAAGQAAPTGRRRAYL
                     GAWVEVPVYRWDALRSGSTIPGPALFESATTTVLALAGERVHVTPHGWLDIRLDASAP
                     HLPAPPGVRVPSASS"
BASE COUNT          359 a          870 c          833 g          334 t
ORIGIN      
        1 ggggcggggc ttggccgtgg aggcggggca tgcgataatg accttcgcgt ttgacacgct
       61 cggcgcgaaa gccctttttg ccggtcatca cccagcgaat acagcatcgc gggcagtgct
      121 agagaaactt ggtttctgct gcacgcacga agagttctac ccagcgactg gtctgaacca
      181 cccgtcctac ctgttgacgc gaccctagaa gcatcaaatc caaagcactc tgcccagttc
      241 tctccaagtt gatgacccaa gactactgcc gcgtcgtccc cgattacgaa gacgcggacg
      301 ggacgcggac accggggggc gccggcaggt gaggtgcgct cgcgtcgagc cggatatcga
      361 gccacccgtg cggggtgacg tgcacccgtt cgccggcgag ggccaggacc gttgtcgtcg
      421 cagactcgaa gagcgccggc ccggggatcg tggaccccga tcgcagcgcg tcccagcgat
      481 agaccggcac ctcgacccat gccccgaggt acgctctccg gcgcccggtg ggcgccgcct
      541 gccccgcggc accgatcggg gactccacag ggacgaccgg gagctcgccg acgacggtca
      601 cccgggcatt gaccaggacg atgtcctgtt cgggcgcgct gtacgtatag agcgcctcgt
      661 ggcggcgctg gaaccgcccg acgatctggt cgatcaggtc gggcgcggcc atatccagac
      721 cgtcgaggga gacggtgatc tcgaagatct gctcgccgta gcgcatgtcg accgcccgtc
      781 gcatcgtcat cgggccggta aacgcctgcc cgaagcgcgt gcgtccttcc gcctccatct
      841 ccgcgaacaa ccgccggacg ccgccgcccc ccacccggtg gatctccccc acgtgcgtcc
      901 ggacgagctc gtaccggagg tcggtggtca gcatccccca cgccgagagg accgcggccg
      961 cccgggggac gatcacccgg gtgatctcca gttggcgggc gacctgggtc acatggaggc
     1021 cggctgcgcc gccgaacgcg agcaccgtga accggcgcgg gtcgacgccc cggcgcaccg
     1081 acacgcggcg gatcccttcc gccatgttcg tgttcacgac ccgatggatc ccctccgcgg
     1141 cggcgacgac cgggcatccc agttggcggg cgacgcgcgc gaccgcgcgg ccggctgcct
     1201 cggggtcgag tcgcgcccgg ccgccgagaa agttcgcggg atccaagagc cccagcacca
     1261 cgttcgcgtc ggtcaccgtc gcccccgttc cgcctttccc gtagcacgcg gggccgggat
     1321 cggcgcccgc gctgtcggga ccgacgtaga ggaggccgcc ggccacccgg gcgatcgagc
     1381 ccccgcccgc gcccaccgtg tggatgtcca ggctcgggag cgcgaccttg tgcccgctca
     1441 ccacccgatc gcccgcgagc ggcggctcgc cgccttccag cagcgcgata tccgtgctcg
     1501 ttccccccat gtcgaacgtg atcagatcct gttggtccag caggtggccg cagagccggc
     1561 tcccggcaag gccgcccgcg ggaccggaga ggatggcgcc ggccgcaagg cgcgccgagt
     1621 cccggatggg cgcgaccccg ccgtgggact gcatgatcag gacctcgcgg cggtatccgg
     1681 cggcggcgag gcggccggcc agggccccga ggtaccggga gagcaccggc ccgacgtagg
     1741 cgttgaccac ggtcgtgcag atccgttcgt actccttgat ctggggaagg acctcgaacg
     1801 aggtacagac gtacgcgcgg gggagtcgcc cggccaccac ccggcgggtc gtccgttcgt
     1861 ggcgggggtt ccggtacgcg tggaggtagc acaccgccac ggcctcgacg tcctcccggg
     1921 cgagggtgcg gatcgccgca tccagggacc gccggttgag cggcgcggcc accgtcccat
     1981 cgaaccggac gcgctcgcgg accccgaggc ggagggcgcg cgggaccagc ggcaccggcg
     2041 ggggcatccg cagattgtag cggtcttcct tcaacccctc gcgcatctcg agaatatcgc
     2101 ggtggccttc cgtcgtgagc atccccacgc gggccccctt gcgctccacc agggcgttcg
     2161 tcgccacggt ggtcccgtgc acgagccggt cggtctccgc gaggagcgcc ccgagatcgc
     2221 gtcccacctc gcgggcgagg aggccgagtc cgtccatgat cccgcgggag ggatcgtccg
     2281 gggtcgaggc cgatttggcc agggcgaccc ggccgttgtc gtcgacggac acgaggtcgg
     2341 tgaacgtgcc cccgacatca atgccgatcc ggtacacgcc gcccccctgc ggtgcg
//
LOCUS       DASQGH010000567         7008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_33629, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000567 DASQGH010000000
VERSION     DASQGH010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33629"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1458)
                     /locus_tag="VEW91_11180"
     CDS             complement(25..1458)
                     /locus_tag="VEW91_11180"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYM92185.1"
                     /translation="MNASYLGQPESGARGRLIPGWSSLAGESVAGPWRRDRLLAALPI
                     AVITGILIGPLFAGDLRPFQAGLFLLGLYILKVTCAAVRATRRSARAAPSEPPPFVSI
                     LIPARNEERVIATTIAQAAAQDYMGPDERPLFEILAIDDASTDGTGGILASLATRYDH
                     LRILTRTAMDGGGKAAALNAALPLCRGEVIVVFDADILIARDFLRTLIAALDPSVAGV
                     QARVRIYNRDSHLLAACQDDEFAAFHKVMQLGRDALDGAVGLGGQGQAVRASALHHVG
                     GWHTGASTEDLDLTVRLVLAGFRVRFCPHAVVWQEGVTNFLALFRQRRRWAAGNLRVC
                     FTYLWPLLGARIALRRRLDYAVYLLSLLTPFWLLLSYLVVTLRQAFGAEPTLALAPIY
                     IITGAIAYFPTLLFGLWGEVTRSPRDLIWRVIRLWGYSFLWIPIGLAALWQTLVVRAP
                     VWDKTFHMGLHPGERILIGQGAIDGDG"
     gene            1685..2101
                     /locus_tag="VEW91_11185"
     CDS             1685..2101
                     /locus_tag="VEW91_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HYM92186.1"
                     /translation="MRFPLPELYHPLVVHFPIGLWLASTLFDLLGWRRAKAVYRDMAF
                     WLVGLGLLGAAASILFGWTDLLAQERRGVGTGLLLQHRVHSWVAYVATATYLGVFVWR
                     WRTANRPVPGWLGLSLLGAGLTLITDYLGGQLRNVM"
     gene            2098..5019
                     /locus_tag="VEW91_11190"
     CDS             2098..5019
                     /locus_tag="VEW91_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYM92187.1"
                     /translation="MNTAPPRTLDLVHELRRVVRGEVRFDAVSRALYSTDASIYEIEP
                     IGVVLPRDADDVQAVLEVTRRARLPVLPRGGGTSLAGQAVGHAVVLDFSKYMNRIAEV
                     NVEAGWARVEPGVVRSELVAALAPHKMVFGPDTSTANRATIGGMIGNNSAGSRSIVYG
                     KTVDSVLSVRALLAGGEAVTLGPLGPEEVAVRARREGPEGNLYRRIPGIIERNREEVA
                     RRYPKIQRRVGGYNLDEFPPGGPVNLAKLVVGSEGTLATVTEATVRIVPRLAATVVAV
                     LHFDDLIGALEVTTEILDTQPTAVEVIDKIVLDMARQMRDYSKKMTFVEGDPEALIAV
                     EYGGESREALLPRLDALEARMRRRGFRGAIVRAIEPAAQANLWQIREAGVGLLLGMKS
                     ARKPVAFVEDTAVAPEHVAEYVRRFREIVERHGTRASFYGHASVGLLHARPILDLKDA
                     HDVATMRQIAGEVSDLVQEFGGAMSGEHGDGLSRSHFNEKLFGRQLYQAFREVKAAFD
                     PEGRMNPGKIVDAPPMTESLRLSPTSRTLSIPTIQDFTRDGGFASAVELCSGVGACRK
                     VGSGTMCPSYMVTLAEEHTTRGRANALRAVISGRLPPEALVGRGLYEVMDLCIGCKAC
                     KAECPSNVDMAKLKHEFLAHYYGAHGIPLRARVFGHAAALGRLGCATAPVSNWVLGST
                     PARWLLHHTVGIHARRRLPGFARQRFSRWFSTRRRPPAAARGGAGGGPDGGRVALLVD
                     TFTEFYYPSVGQAAVRLLEAAGCQVLLAPAGCCGRPMISNGMLGAARSLARRNTHRLR
                     GLAEEGVPIVGLEPSCAVTLRDEYPDLIPGEAAEAVARQTVMIEEFLVALHERGVRLR
                     FAGQERLILLHGHCHQKAIVGTQPSLAALRWLPGATVREVDSGCCGMAGSFGFESEHY
                     EVSLAMGERVLFKAVRDLPREALVVAAGVSCRQQILHGTGRRAVHLVEALAGALDAPG
                     G"
     gene            5042..5116
                     /locus_tag="VEW91_11195"
     tRNA            5042..5116
                     /locus_tag="VEW91_11195"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5074..5076,aa:His,seq:gtg)
     gene            5144..5356
                     /locus_tag="VEW91_11200"
     CDS             5144..5356
                     /locus_tag="VEW91_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92188.1"
                     /translation="MVVQDDRPAMTGIVVEFDGRARRWVASCDDPGGEERLIVNAETH
                     ERAQRLLDERWRARLELLDREGEEER"
     gene            5392..6189
                     /locus_tag="VEW91_11205"
     CDS             5392..6189
                     /locus_tag="VEW91_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYM92189.1"
                     /translation="MAGPAFEVITAPNPSPFTLAGTNTYLIGTGGDVVVIDPGPDDAG
                     HVTHLLEQAAASGRRVVLALLTHGHSDHRGAADRLADTTGAPVRGWNTPQAPLRDGEE
                     LALPGGRLRVMHTPGHAPDHVAFHWEERGVLFSGDLILGEGTVQVTPPGGSMVDYLRS
                     LELVARLDLTMIAPGHGPLVRKPHRRIAEYLAHRRAREQQVLDALAAGARTAREVAAM
                     IYPDLDPRRRAAAEGTVFAHLEKLVLDGRARRSGARFGAVTRRRGRW"
     gene            6183..6410
                     /locus_tag="VEW91_11210"
     CDS             6183..6410
                     /locus_tag="VEW91_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92190.1"
                     /translation="MVIPKAAEETLHDRLGHPIRVGDAVRIAGLPDLAEVQAVDPRYG
                     VVIVLVPGRTGKMGRMLRAQEVERAGAAAPR"
     gene            complement(6462..>7008)
                     /locus_tag="VEW91_11215"
     CDS             complement(6462..>7008)
                     /locus_tag="VEW91_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HYM92191.1"
                     /translation="KLQERLAKLSGGVAQIKVGAATETELKEKKHRFEDALSTSKAAV
                     EEGIVAGGGVALLNIAKALDKVDADDADEKSGVSIVRKAIDEPARWLAANAGMEGAVV
                     VEHIKAEKPGIGYDVAEGRFTDMMKAGIIDATKVTRLALQNAASVASLLLTTEALVVE
                     KREKKRAPAAPGGYNPEDMEL"
BASE COUNT         1035 a         2390 c         2538 g         1045 t
ORIGIN      
        1 gtagggacga cagcgtcccg ggcatcaccc gtcgccgtcg atcgccccct gcccgatgag
       61 gatccgttcg ccggggtgca gccccatgtg aaacgtcttg tcccagaccg gtgcccgaac
      121 gaccagggtc tgccacaggg ccgcaagacc gatcgggatc cacagaaagc tgtaccccca
      181 cagccggatc acccgccaga tgaggtcgcg cggggaccgg gtcacctccc cccacaggcc
      241 gaacagcagc gtcggaaagt atgcgatggc gccggtgatg atgtagatcg gcgccagggc
      301 gagcgtcggc tcggcgccga aggcctgccg gagggtgacg accagatacg acagcaggag
      361 ccaaaacggc gtgaggaggc tcaggaggta caccgcgtag tctagcctcc gccggagcgc
      421 gatccgggcg ccgagcagcg gccacaggta cgtgaagcac acgcgtagat ttcctgccgc
      481 ccaccggcgg cgctgccgga agagggccag gaagttcgtc accccttctt gccagaccac
      541 cgcgtgcggg cagaacctga cccggaaccc cgccaggacg agccggaccg tcaggtcgag
      601 gtcttcggtg gatgccccgg tgtgccaccc tccgacgtga tgaagggcgg aggcccggac
      661 ggcctgcccc tgtcccccca ggccgaccgc gccgtccagc gcgtcgcgcc caagctgcat
      721 caccttgtgg aacgccgcga actcgtcatc ctggcaggcg gcgaggaggt gcgaatctcg
      781 attgtagatc cgcacgcgcg cctgaacgcc tgcgaccgat gggtcgaggg cggcgatcag
      841 cgtccggaga aaatcgcgcg caatcaggat gtcggcatcg aagacaacga tcacctcgcc
      901 ccggcacagt ggcaacgccg cattgagcgc cgccgccttt cccccgccgt ccatggccgt
      961 ccgggtcagg atccgcaggt ggtcataccg ggtggcgagc gacgcgagaa tgccgcccgt
     1021 tccatcggtc gaggcgtcgt cgatggcaag gatctcgaac aggggccgct cgtcgggccc
     1081 catgtagtct tgagccgccg cctgcgcgat cgtcgtggcg atcacccgct cctcgttcct
     1141 cgcggggatc aggatcgaga caaacggagg aggttccgac ggcgcggcac gggccgagcg
     1201 ccgcgtggcc cggaccgcgg cgcaggtcac cttgaggatg tagagcccga ggaggaacag
     1261 gccggcctga aagggtcgga gatctccagc aaagagcggc ccgatcagga tgccggtgat
     1321 gaccgcgatc ggcagggcgg caagcaggcg atcccgtctc cacggcccgg cgaccgactc
     1381 cccagccagt gacgaccagc cgggaatgag ccggccacgg gcgcccgatt ccggctgtcc
     1441 caggtatgag gcgttcatgc gcggaggatg tcccggggcg gcgcagcgca cgggggaagc
     1501 atgcccccgt cttcgcggcg gttcgccgtc agtcctggtg ggagagcgcg ggacctggag
     1561 gttcaacggc cgcgcgaaga gaaccatcgg gcgtcgtggg cgcgatccac ccggaagcgt
     1621 cgatggggca catccacccg acggtggccg tcatgcgcga ttcgcttggc gggagatcgg
     1681 ggtaatgcgc tttcctctcc ccgagctcta tcatcccctt gtcgtgcatt tccccatcgg
     1741 gctctggctc gccagcacgc tctttgacct gctcgggtgg cggcgcgcga aggccgtcta
     1801 ccgggacatg gcgttctggc ttgtcggcct cgggctcctc ggcgcggccg cgagcatcct
     1861 gttcgggtgg acggacctcc tggcccagga gcgccgaggg gtcggaaccg ggttgctcct
     1921 ccagcaccgc gtgcacagct gggtcgcata tgtcgccacc gccacgtacc tcggggtctt
     1981 cgtctggcgc tggcggacgg cgaaccggcc ggtccccggg tggctggggc tgtccctgct
     2041 gggtgcgggg ctgaccctca tcaccgatta tctcggcggg caactgcgga acgtaatgtg
     2101 aacacggcac cgcctcgaac cctggatctc gtccacgagc tgcgccgggt cgtccgcggc
     2161 gaggtccggt tcgacgccgt ctcgcgggcg ctgtacagca ccgacgcgag catctacgag
     2221 atcgagccga tcggggtcgt cctcccccgg gacgccgacg atgtccaggc cgtgctggag
     2281 gtgacacgtc gagcccgcct cccggtgctc ccgcggggcg gcgggacgag tctggcggga
     2341 caggcggtcg ggcacgccgt ggtgctggac ttctccaagt acatgaaccg gatcgccgag
     2401 gtcaacgtgg aagccggctg ggcccgcgtc gagccggggg tggtgcgcag cgagctggtg
     2461 gccgcgctgg ccccgcacaa gatggtcttc gggcccgaca cgtccaccgc aaaccgggcc
     2521 acaatcggcg gcatgatcgg gaacaactcc gccgggtcac gctcgatcgt gtacggcaag
     2581 acggtcgaca gcgtgctgtc ggtccgggcc ctgctcgcag ggggggaggc cgtgaccttg
     2641 ggcccgctcg ggcccgagga ggtggcggtc cgcgcgcgcc gtgaagggcc ggaggggaac
     2701 ctgtaccgcc ggattccggg gatcatcgag cggaaccgtg aggaggtggc gcgccgctac
     2761 cccaagatcc agcggcgggt cggcgggtac aacctggacg agttcccgcc ggggggcccg
     2821 gtgaacctcg ccaagctggt ggtgggttcg gaggggacgc tcgccacggt gacggaggcc
     2881 acggtcagga tcgtcccgcg gctcgcggcg acggtggtgg cggtgctgca cttcgacgac
     2941 ctgatcgggg cgctggaggt cacgacggag attctggaca cccagccgac cgcggtggaa
     3001 gtgatcgaca agatcgtcct ggacatggcc cggcagatgc gcgactactc caagaagatg
     3061 acgttcgtcg aaggagatcc ggaggcgctg atcgccgtcg agtatggcgg ggagagccgt
     3121 gaggcgctcc tcccccgcct tgatgcgctc gaagcgcgga tgcggcgacg cgggtttcgc
     3181 ggagccatcg tcagggcgat cgagcccgcg gcgcaagcga acctctggca gatccgggag
     3241 gccggggtcg gcctcctgct gggcatgaag tcggcgcgaa aaccggtcgc cttcgtcgag
     3301 gacacggcgg tggcgcccga acacgtcgcg gagtacgtcc ggcggtttcg cgagatcgtc
     3361 gagcgccacg ggacgcgcgc gtcgttctac gggcatgcca gcgtcggcct cctgcatgcc
     3421 cggccgatcc tcgacctcaa ggacgcgcac gacgtcgcca ccatgcgtca gatcgctggt
     3481 gaggtcagcg acctggtcca ggagtttggg ggggcgatga gcggcgagca cggggacggg
     3541 ctgtcgcgca gccacttcaa cgagaagctg ttcgggcgcc aactgtacca ggcgttccgc
     3601 gaagtcaagg ctgcgttcga tcccgagggg cggatgaacc cggggaagat cgtcgacgcc
     3661 ccgccgatga ccgagtcgct gcgcctcagc ccgacctccc gaacgctctc gatccccacg
     3721 atccaggact tcacgcgaga cggtggattc gcgagcgcgg tggagttgtg cagcggggtg
     3781 ggggcctgcc gcaaggtggg gagcggcacc atgtgcccct cctacatggt gacgctggcc
     3841 gaagagcaca ccacacgggg tcgggcgaac gccctccgcg cggtgatctc cggccgcctt
     3901 cccccggagg ccctggtcgg ccgcgggctg tatgaggtga tggatctctg catcggctgc
     3961 aaggcctgca aggcggagtg ccccagcaac gtcgacatgg ccaagttgaa gcacgagttt
     4021 ctggcccact attacggggc gcacgggatc ccgctcaggg ccagggtatt cggccacgcg
     4081 gccgccctcg gacggctggg gtgcgcgacc gcccccgtct ccaactgggt gctagggagt
     4141 acgccggccc ggtggctcct ccatcacacc gtcggcatcc atgcccggcg gcgccttccc
     4201 gggttcgccc gccagcgctt ctctcggtgg ttctccaccc gccgccgccc gccggccgcc
     4261 gcgcggggag gggcaggggg gggtcccgac ggcggtcgcg tggccctgct cgtggatacc
     4321 tttaccgagt tctactaccc atccgtgggg caagcggcgg tccggctgct ggaggccgcc
     4381 gggtgtcagg tgctcctggc gcctgccggg tgctgcggcc gaccgatgat cagcaacggg
     4441 atgctgggcg cggcccgctc actcgcccgc cgcaacacgc accggctccg ggggctcgcg
     4501 gaggagggcg tccccatcgt cggcctggag ccgtcgtgcg ccgtgacgct ccgggatgaa
     4561 taccccgatc tcatcccggg cgaggccgcg gaggccgtcg cccgccagac ggtcatgatc
     4621 gaggagttcc tcgttgcgct ccacgagcgc ggcgtccgcc tgcggtttgc cggtcaggag
     4681 cgcctcatcc tcctgcacgg ccactgtcac cagaaggcga tcgtggggac gcagccctca
     4741 ctggccgcgc tccggtggct gcccggggcc accgttcgcg aggtcgactc cgggtgctgc
     4801 gggatggccg ggtcgttcgg attcgagtcg gagcactacg aggtctcgct cgcgatgggg
     4861 gagcgggtgc tcttcaaggc cgtccgcgac ctccctcggg aggcgctggt ggtggccgca
     4921 ggtgtgtcgt gccggcagca gatcctgcac gggacgggcc gacgcgcggt gcacctggtg
     4981 gaggcgctgg ccggcgctct ggacgctccg gggggctgat gttacaatgc aggcggtcgc
     5041 ggtggccgta gctcaagggc agagcaccag actgtggcgc tgggggttgc gggttcaagt
     5101 cccgtcggcc accccagccc aagccttccg atggaggtcc accatggtgg tccaagacga
     5161 ccgtcccgcg atgaccggga ttgtggtgga gttcgacggc cgcgcccgcc ggtgggttgc
     5221 gtcgtgcgac gaccccggcg gggaagaacg cctcatcgtc aacgccgaaa cgcacgagcg
     5281 cgcgcagcgg cttctcgacg agcgatggcg tgcccgcctg gaactcctcg accgcgaagg
     5341 cgaggaggag cgatagggac cggaccccgc agccgtcgtt ccggggccgg cgtggccggc
     5401 ccggcgttcg aggtgatcac cgccccaaac ccgtcgccgt tcacgctggc ggggacgaac
     5461 acctacctga tcggaacggg cggggacgtg gtggtcatcg acccgggtcc ggacgacgcg
     5521 gggcatgtga cccacctgct cgagcaggcc gccgcctcgg gccggcgcgt ggtgctggcc
     5581 ctcctcaccc acggccacag cgatcaccgg ggcgccgcgg atcgccttgc ggacaccacc
     5641 ggggcgccgg tgcgggggtg gaacaccccg caggccccgc tccgcgacgg cgaggagctc
     5701 gcactccccg gcgggcggct gcgggtgatg cacaccccgg gccacgcccc ggaccacgtg
     5761 gcgtttcact gggaggagcg gggcgtcctg ttctcggggg acctcatcct cggcgagggg
     5821 accgtccagg tcaccccccc ggggggatcg atggtggact atctgcgctc gctcgaactc
     5881 gtggcacgcc tcgacctcac gatgatcgcc cccggccacg ggccgctggt ccggaagccg
     5941 caccggcgga ttgcggagta cctggcgcac cgccgcgcgc gcgagcagca ggtgctcgac
     6001 gccctggccg cgggcgcgcg gacggctcgg gaggtggcgg cgatgatcta tccggacctt
     6061 gatccgaggc gccgggcggc ggcggagggg acggtctttg cacatctcga gaagctggtg
     6121 ctggacgggc gggcgcgacg cagcggcgcg cgctttgggg ctgtgacccg caggaggggc
     6181 cgatggtgat tccaaaggca gcggaagaga cgctccacga ccggctgggc cacccaatcc
     6241 gggtgggcga tgcggtccgg atcgccggcc tcccggatct ggcggaagtg caggcggtgg
     6301 atccccggta cggcgtcgtg atcgtgctcg tcccgggccg gacgggcaag atgggccgga
     6361 tgctccgggc gcaagaggta gagcgcgccg gcgcagcggc tccgcgataa aagcagagga
     6421 ggggccacgc ggcccctcct ctccccccgg gtgcggggcg gtcacaattc catgtcctcc
     6481 gggttgtagc cgcccggggc ggccggcgcc ctcttcttct cccgcttctc caccacgagc
     6541 gcctcggtgg tcagcagcag gctcgcgacg ctcgccgcgt tctgcagcgc caacctggtc
     6601 accttggtcg cgtcgatgat cccggccttc atcatgtcgg tgaaccggcc ctccgcgaca
     6661 tcgtagccaa tcccgggctt ctccgccttg atatgctcga cgacgaccgc tccctccatc
     6721 ccggcgttgg ccgccagcca cctggccggt tcgtcgatcg ccttgcggac gatgctgacc
     6781 ccgctcttct cgtcggcgtc gtcggcgtcc accttgtcca gggccttggc gatgtttaac
     6841 agcgcgacac cgcctccggc gacgatcccc tcctcgaccg cggccttgct cgtgctgagc
     6901 gcgtcctcga accggtgctt cttctccttg agctccgttt cggtggcggc gccgaccttg
     6961 atctgggcga cccctccgga gagcttcgcg agccgctcct gcagcttt
//
LOCUS       DASQGH010000568         6658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36491, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000568 DASQGH010000000
VERSION     DASQGH010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36491"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1112)
                     /locus_tag="VEW91_11220"
     CDS             complement(153..1112)
                     /locus_tag="VEW91_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM92192.1"
                     /translation="MIQADALSRSFGERWAIQDVSFEAHPGEILGFLGPNGAGKTTTM
                     RILAGFLPPTSGRASVAGHDVVEKPLEAKRQLGYLPEVIPLYEDFTAVEYLRFVARLK
                     GVDRRKVNGAVDQAMALCAVTHVAHRLVRNLSRGYRQRLGLAHAIVHDPPVLILDEPT
                     SAMDPRQIVEIRNVIKGLRGSHTIILSTHILPEATALCDRVIIINEGRVVAVDTYEQL
                     AARLRSSEKTLVRVVRLDTGLTDRLRNLPGVVHVTPGPAAGELVVEAVLGRDVREEIA
                     RAIAGTGAGLLELRPLAMSLEDVFLRLVTHEEAPAVGAEGRRG"
     gene            1269..2834
                     /locus_tag="VEW91_11225"
     CDS             1269..2834
                     /locus_tag="VEW91_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HYM92193.1"
                     /translation="MAALVTGLAGAAPASAARAAPPRLALWMEPGANLAVLSTLEGVR
                     QTLDQARQAGVDVVIPEAKNAWGYVTYPSAFAPTIDASPILRHGFHHPPLPQGYPEGF
                     DMLGTIVREAHARGMRVDAAVNTFNEGFSRLQVGPAFDHPEWQATAYLGTRPVIAPDG
                     TSYDLAGVDIPRAGDALVLYTPAAGATTPTSRWGTEVAVASGEVTEVRDRAAGDTDPG
                     PTPIPAGGYVLSGHGAAAGWLARALPVGANVAIGPIQTRMAPSSAHSPYAFVNPADPE
                     VSGYEMAVIYEVLSRYDVDGIILDRARYQDITEDFSPLSRAGFEAFIGQKVGHWPEDI
                     YTYAPSGYWVTRVSGPLYRAWLGYRAHTILAYVRAVAHLVHTFRPQVAVAMYVGAWYP
                     VYYDEGVNWASPEVAAPYAWIGPEWVRAGLAPLLDYLMIGLYYQDVTAGEARASHHNP
                     EVSVQGGALLARSLVQGATPLVGSLQVSLYAGDPGRLTRAVRMSQGLTRGTMLFDLVY
                     LTQDDLWNALPRP"
     gene            complement(3196..3606)
                     /locus_tag="VEW91_11230"
     CDS             complement(3196..3606)
                     /locus_tag="VEW91_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HYM92194.1"
                     /translation="MRYLTTEQLKTVNHRMIRATGGLYLASEQNVVYPPSLDSLVSFV
                     QTRIALRPQPSVWEMAAFYVDRLTRDQVFHDGNKRTALEAARLFLEGAGYRLSLTPAN
                     ESVEFVTNVARGGLNRDTIADWLRGHSRRKARKM"
     gene            complement(4181..4384)
                     /locus_tag="VEW91_11235"
     CDS             complement(4181..4384)
                     /locus_tag="VEW91_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYM92195.1"
                     /translation="MPKGTVKWFNREKGYGFITPEEGKDVFVHYTGIAGEGFRNLEEG
                     QIVEFEITQGQKGPQAQNVRVVG"
     gene            complement(4470..5750)
                     /locus_tag="VEW91_11240"
     CDS             complement(4470..5750)
                     /locus_tag="VEW91_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM92196.1"
                     /translation="MTEVRVQIAVVGGGPAGLAAAGECHAAGARVLLLEERPVLGGRA
                     VIVPGARGLAEGLMRDLRAAEVWRSSPVWGLWGHTLAVLRDGRPRTATAEAIILATGA
                     VEELVPFPGWSLEGVMTVEAAWEAVRAGKVGPSSGPGVVVGGAEAGTLATRLSERGTA
                     VTLVAAERPKGVPDSIPVVPGILAAARGEGAVQRAVLADGTEHECRMLCIESPRIPLT
                     DVARLAGAPCVYQPRLGGFVPRYDRTMALHAPTPALYIAGDAGGIDTPRAAAESGRLA
                     ARSALHALKLLPDPEARIEESWRQLRAASAPLRARARESLVIGAMPDEVVEQWDGPAE
                     TIFCPCNGVTVEALQAAVDGGARSPDDLKRMTRCGMGMCQWRRCGPAVMRWLSGTLGV
                     PVGRLPLPRVRPPLRPVPVSAFAEFGEGGGPSGG"
     gene            complement(5747..>6658)
                     /locus_tag="VEW91_11245"
     CDS             complement(5747..>6658)
                     /locus_tag="VEW91_11245"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYM92197.1"
                     /translation="WLGGIEPASACVRLNTDGTLQVVVGSVDISGTATGLAQVAAEML
                     GTPVSSVRLVGADSDSAPASGMSGGSKIMYTVGSAVAKAAQDARRQILALAARRLEAA
                     EGDLEIADGVVRVRGVPARTVAIADLAKLTSGFGAGHPPVFGVASEAIVQRAPAFGAH
                     IARVRLDADSGRVRVVDYAVAQDVGRAINPAAIHGQIHGGVAQGIGWALLEQVVYDEH
                     GSVATGSFLDYALPRASDVPTIRATLVEVPSERGPYGAKGVGEPPVVPVAAAIANAIA
                     DASGARIEALPMVPEAVTAALARSGRA"
BASE COUNT         1067 a         2430 c         2127 g         1034 t
ORIGIN      
        1 cccctggagc tggagcacct ggagcgagta gaatccgatg agcgagaaga acaggtagcc
       61 cacgacgaca aagaacatgg ccagcgcgac atacgcgatc ggggaactga aaatctggcg
      121 gagctccttg agggcaatga cgagggcgcc cctcatccgc gccgtccctc cgcccccacc
      181 gcaggcgcct cctcgtgggt gaccaggcgc aggaagacat cctccaggct catcgccagc
      241 ggccgtagct caagcaatcc cgcccccgtc cccgcgatgg cgcgcgcaat ctcctcgcgc
      301 acatccctcc cgaggacggc ctccaccacc agttcgccgg ccgccggacc aggggtgacg
      361 tgcaccaccc cgggaagatt ccggagccgg tcggtcaggc cggtgtccag gcggacgacg
      421 cgcacgaggg tcttctccga actcctgagg cgggccgcca gctgctcgta ggtgtccacg
      481 gccaccaccc ggccttcatt aataatgatc acgcggtcgc acagcgccgt cgcctcgggc
      541 agaatgtggg tgctgagaat gatcgtgtgg cttccgcgca ggcctttgat gacgttccgg
      601 atctcaacga tctggcgggg gtccatcgcg gaggtgggct cgtcgaggat gagcaccggg
      661 gggtcgtgga cgatggcatg ggccagcccg agccgctgcc ggtacccccg ggagaggttg
      721 cggacgaggc ggtgggccac gtgtgtgacc gcgcagagcg ccatcgcctg atcgaccgcc
      781 ccgttgacct tccggcgatc cacgcccttg agacgcgcca cgaacctcag gtactcgacc
      841 gctgtgaagt cctcatagag gggaatgact tcggggaggt atcccagttg gcgtttggct
      901 tcgagcggct tctccaccac gtcgtgcccg gccacggacg cccggccgga cgtgggaggg
      961 aggaagccgg ccaggatccg catcgtcgtg gtctttccgg cgccgttggg gccgagaaat
     1021 ccaaggatct cccccggatg cgcctcgaac gacacgtctt gaatcgccca ccgctcgccg
     1081 aagctgcggg acagcgcgtc ggcctggatc acaattcacg actccccttc acaagaaatc
     1141 gcttggtcgg gcaacagcct ctatcctaat ctgggaaccc cccagtttgc aaggggtgaa
     1201 ggagcctctg gcgggtcttc gaacttcttc gtatgcacgt ctccgcccgc ttgggtgcca
     1261 cgtgcctcat ggccgcgctc gtcacgggcc ttgcgggagc cgcccccgcg tcggccgccc
     1321 gcgcggcgcc cccccggctt gcgctgtgga tggaacccgg cgccaacctg gccgtgctca
     1381 gcaccctcga gggggtgcgg cagaccctgg accaggcgag gcaggcgggg gtggacgtgg
     1441 tgatccctga ggccaagaac gcctggggat acgtgacgta cccgagcgcg tttgcgccca
     1501 caatcgacgc ctcgccgatc cttcgccacg ggttccacca cccgcccctg ccccaaggct
     1561 accccgaagg attcgacatg ctcgggacga tcgtccggga agcacacgcc cggggtatgc
     1621 gggtggacgc cgccgtcaac acctttaatg agggattctc gcgtctccag gtcgggcccg
     1681 cgttcgacca tcccgagtgg caggcgacag cgtacctggg cacgcggccc gttatcgctc
     1741 ccgacggaac gtcgtacgat ctcgccgggg tggacatccc ccgagccggg gacgcgctgg
     1801 tgctctacac gccggcggcc ggcgccacca ccccaacctc ccggtggggg accgaggtcg
     1861 cggtcgcgtc gggggaggtc accgaggtgc gcgaccgggc ggccggggac accgaccccg
     1921 gcccgacgcc gatcccggca ggaggatatg tgctctcggg ccacggtgcc gccgccggct
     1981 ggctcgcccg cgccttgccc gtgggggcca acgtcgccat cggaccgatc caaacccgca
     2041 tggccccgtc atccgcccac agcccctacg ccttcgtgaa tcccgccgac cctgaggtct
     2101 ccgggtacga gatggccgtc atctacgaag tgctcagccg atacgacgtg gacgggatca
     2161 tcctggatcg ggcccggtac caggacatca ccgaggactt ctcgccgctc agccgcgccg
     2221 gctttgaggc gttcatcggg cagaaggtgg gtcactggcc cgaggatatc tatacctacg
     2281 caccaagcgg gtactgggtc acccgggtgt ccggtcccct gtaccgagcg tggctcggct
     2341 accgggcgca cacgatcctg gcctacgtac gcgccgtcgc ccatctggtg catacgttcc
     2401 ggccccaggt ggcggtcgcg atgtacgtcg gggcctggta cccggtgtac tatgacgagg
     2461 gagtgaactg ggcgagcccc gaggttgccg ccccgtatgc gtggatcggg ccggaatggg
     2521 tccgcgcggg cctcgccccc ctcctcgact atctcatgat cgggctgtac taccaagacg
     2581 tcaccgctgg ggaggcgcgg gccagccatc acaatcccga ggtcagcgtc caggggggag
     2641 cgctcctcgc ccgctccctg gtccaggggg cgaccccgct cgtaggatcg cttcaggtct
     2701 ccctgtacgc cggggatccc gggcggctca cccgggcggt tcgaatgtca caggggctca
     2761 ctcgggggac gatgctcttc gacctggtct accttaccca ggacgatctc tggaacgcgc
     2821 tcccccgacc gtgaacagct gacgcttctc cgacccgcgg gaccctccac aacggagaag
     2881 gaccccggat cggggccctt ctcggcacga aaagacctag cgagcctgct gcgctccctc
     2941 aggacctgcg agcagaggaa acgccggttt gccctccagc cgcaccgtcg tcgtccagct
     3001 gagccgcagc cgaagcgccg gccggaatag gcggattcgc tatccggtca tcacccgact
     3061 aggcggatac tggcggcagg tcggacggca cggcgtggag cgtctactat ggtccatccg
     3121 catcacctcc tctctcgttt accccaatca tcgctcccgg ggtcgccccg cggtcaccat
     3181 gggcagaccg ggatcctaca ttttcctggc cttccgtctt gagtggcccc gaagccaatc
     3241 ggcgatcgtg tcgcggttga gcccgccgcg ggccacgttg gtcacgaact cgacggactc
     3301 gttcgcaggg gtgaggctca agcggtagcc cgccccctca aggaacagac gggccgcctc
     3361 cagcgcagtc cgcttgttgc catcatgaaa cacctggtct cgcgtcaatc ggtcgacgta
     3421 gaatgcggcc atctcccaga ccgagggctg ggggcgcagc gcgatccggg tttggacgaa
     3481 gctcaccagc gagtcgaggc tcgggggata gacgacgttc tgctcgctgg cgaggtagag
     3541 acccccggtg gcccggatca tccggtggtt gacagtcttg agttgctcgg tggtcagata
     3601 cctcaccgct tcgccaatgc ctcccaaacg gtccggaagt cgcgcaggct ccgttcgagg
     3661 ctcccagaga acattcgttc gctcagaggc tcaggggcgg gcaagggccg cccgctgcgg
     3721 cgcgccctca cgtgcctgga tcgcacgatt ctcacgcgtg cccgcggagc cgggcgactc
     3781 cggacgatcc ggcgccttct tgtcgccgat ttcgccctgg atcgacgcaa tttgccccga
     3841 tacgcgggct tcgcccgccg cacatgcgtt tttgcggagc cgcgccgcat agaatcctcc
     3901 tcgaattaat ctagcatggc cataaagtgg gcgcaatccc cattgagggg gaactcggca
     3961 attcgcttgc ctagggcaat cttggctgga aaggcccagt tgcatgtaca tatgctgtcg
     4021 agcgatttat cgagattgac gaagattgag cgagatttta tagaatttca accgaaattg
     4081 cctggaatcg ccatcctgag gcccatgaca caaaggcacc gggaacggat cgttcccggt
     4141 gcctcagctt tctatgatat gtggcgggtc tgcccgacga ctacccgacg acgcgaacgt
     4201 tctgtgcctg cggccccttt tgcccctggg tgatctcgaa ctctacgatt tgaccctcct
     4261 ccagattccg aaacccctcc ccagcgatac cggtgtagtg aacgaagacg tccttccctt
     4321 cctcgggggt gataaagcca taccctttct ctcgattaaa ccacttgacc gtgccttttg
     4381 gcataccctc ttccttcctc ccctggggcc ggttgcccta gtttacgttc ccctttatac
     4441 cacagcctct ctgcgaatcc tccctccggt cacccgccag aggggccacc gccctccccg
     4501 aactccgcga aggcggaaac cggcacaggg cgcagagggg gccgaacccg gggtaacggc
     4561 aggcgcccca ccggcacgcc gagggttccg ctcaaccacc gcatgaccgc tgggccgcac
     4621 cgccgccact ggcacatccc catcccgcac cgggtcatcc gcttcagatc gtcggggctc
     4681 ctcgccccac cgtccaccgc tgcctggagc gcctcgacgg tcaccccgtt gcacgggcag
     4741 aagatcgttt ccgcgggacc gtcccattgc tccaccactt cgtccggcat cgctccaatg
     4801 accagcgact cgcgggcgcg tgcgcgcagc ggagccgacg ccgcccgcag ctggcgccag
     4861 gattcctcga ttctggcctc cgggtccggc aggagcttga gcgcatgcag ggcggagcgc
     4921 gcagccagcc gccccgactc ggccgccgcg cgcggggtat cgattccccc ggcgtcgcct
     4981 gcgatataga gggccggggt cggagcgtgg agcgccatcg tccggtcgta ccggggcaca
     5041 aaccctccga ggcggggctg gtacacgcag ggcgcgccag ccaggcgcgc gacatcggtg
     5101 agcgggatcc gtggcgactc gatgcacaac atccggcatt cgtgctcggt gccgtcggcg
     5161 agcacggccc gctggaccgc gccttccccc ctcgccgcag ccaggatgcc ggggaccacg
     5221 gggatgctgt ccggcacccc cttgggacgc tcggcggcga ccagcgtgac ggcggtgccc
     5281 cgctcggaca accgcgtggc cagcgtcccc gcctcagccc cccccacgac cacgccggga
     5341 ccgctcgacg ggccaacctt ccccgcccgc acggcttccc aagccgcctc gacggtcatc
     5401 acgccctcca gcgaccaccc gggaaagggg acaagctcct ccacggcccc cgtcgccagg
     5461 atgatcgcct ctgcggtcgc ggtgcggggc cggccatcgc gcaagaccgc gagcgtgtgc
     5521 ccccacagac cccacacggg gctgctccgc cacacctcgg cggcgcgaag gtcgcgcatc
     5581 aacccttcag ccaggccgcg agccccggga acgatcacgg cgcggccgcc cagcaccggc
     5641 cgctcctcga gcagcagcac ccgggcccct gcggcgtggc attcccccgc agccgcgagt
     5701 ccggccggac ccccgcccac gaccgcgatc tggacgcgga cctctgtcac gcccgccccg
     5761 atcgggcaag cgcggccgtg acggcctcgg gcaccatggg cagggcctcg atgcgcgctc
     5821 cgctggcgtc cgcgatcgcg ttggcgatcg cagcggccac cgggaccacc ggcggctctc
     5881 cgaccccctt cgccccgtac ggcccccgct ccgaggggac ctcgacgagg gtggcgcgga
     5941 tcgtcgggac atccgaggcg cggggcaggg catagtcaag gaaggagccg gtcgccaccg
     6001 acccatgctc gtcgtagacc acctgctcca ggagcgccca gccgatcccc tgggccactc
     6061 cgccgtggat ctgcccgtgg atggccgccg gattgatcgc ccggccgaca tcctgcgcca
     6121 ccgcgtagtc gacgacccgc acccgccccg agtcggcatc cagccggacg cgggcgatat
     6181 gcgccccgaa cgcaggcgcc cgctggacga tcgcttcgga ggccacccca aacaccgggg
     6241 gatggccggc cccgaacccc gaggtgagtt tggccaggtc cgcgatcgcg acggtgcggg
     6301 cggggactcc gcggacccgc accactccat cggcgatctc aaggtccccc tcggccgctt
     6361 cgaggcggcg ggcggcgagc gccaggattt gccgccgcgc atcttgcgcg gcctttgcca
     6421 ccgccgagcc gaccgtgtac atgatcttgc tgcccccgga catcccggaa gccggggcgc
     6481 tgtcgctgtc ggccccgacc agccgcacgc tcgagaccgg cgtgccgagc atctcggcgg
     6541 ccacctgggc cagaccggtc gcggtaccac tgatatccac cgagccgacc accacctgca
     6601 gcgtgccgtc ggtgttgagc ctcacgcagg cgctggcggg ctcgatcccc ccgagcca
//
LOCUS       DASQGH010000569         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22161, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000569 DASQGH010000000
VERSION     DASQGH010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22161"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VEW91_11250"
     CDS             <1..245
                     /locus_tag="VEW91_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HYM92198.1"
                     /translation="RADEVLGDVVVRLKKRGIRHPYVKNFVLARTTPLTRQRKTLPSF
                     EQTLKKLTANLEGFDLTKIRNEDIQRAAVIAAPAAE"
     gene            complement(249..638)
                     /locus_tag="VEW91_11255"
     CDS             complement(249..638)
                     /locus_tag="VEW91_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009143990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HYM92199.1"
                     /translation="MGFALFVNGTLMRGLALHVNLAGAEFLGEVRTAPIYRLYSIGDR
                     HPGMFEVPEGGVSVSGELYRVPEEVWRRVEAGEPPGLYRGAVMLEDGRWVDGLLYPRE
                     LAQGRHRDISSYGGWRAYMAAPPMPDR"
     gene            complement(648..>1722)
                     /locus_tag="VEW91_11260"
     CDS             complement(648..>1722)
                     /locus_tag="VEW91_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HYM92200.1"
                     /translation="SMRPAFQMWEVPWIAFISLAIILVSWVANVKLPFGVPGGLAAVV
                     IGTAVGWVAVLLGWSGVLVPSAVGESFRQFGVHLPIPGREVLRGLADIGPLLATAIPL
                     GVYNFTEGMNNVESAAAAGDSYNLRWILLADGTGAVVGSFLGSPFPPAVYIGHPGWKT
                     VGGRIGYSLVTGIVIALVAFLGLVALLLALIPIQALVPILLFIGLVIGAQAFQATPAR
                     HAPAVVLALLPNIALWAKTLVDDALGAAGTDAAKLGLDKLGTGDIYHGMALFGGGAVL
                     AGMVLGALAAFVIDRDFHRAAVAALSGAVLSFVGLINGAGPVGLDVSPDVTLGYLLMA
                     AGFAVAAWRAGPQPHPAGRSGSG"
BASE COUNT          325 a          643 c          554 g          200 t
ORIGIN      
        1 gtcgggcgga cgaggtcctc ggcgacgtcg tcgtcaggct caagaaacgg ggcatcaggc
       61 acccctacgt caagaacttc gtgctcgccc gcaccacccc gctgacccgg cagcgcaaga
      121 cgctgcccag cttcgagcag accctgaaga agctcacggc caatctggaa ggattcgacc
      181 tgaccaagat ccgcaacgag gatatccagc gcgcggcggt gatcgccgcg cccgcggcag
      241 agtagatctc agcgatcggg catggggggt gccgccatgt aggcgcgcca gccgccgtag
      301 gacgaaatgt cgcgatgccg gccctgagcc agctcccgcg gatacaggag tccgtcgacc
      361 caccgcccgt cctcgagcat cacggcgccc cggtacagcc cgggcggttc gccggcctcg
      421 acgcgccgcc atacttcttc gggcacccgg tacagctccc cgctcacgga gactcccccc
      481 tccgggacct cgaacatccc ggggtgccgg tccccgatcg aatagaggcg gtagatgggg
      541 gcggtccgga cttcaccgag gaactccgcc cccgcgagat tcacgtgcag ggccaatccc
      601 cgcatcagcg taccgttgac gaagagagca aatcccacga ctgcggttta cccgctcccc
      661 gacctccccg ccgggtgcgg ctgcggcccg gcacgccacg cagcgaccgc gaagcccgcg
      721 gccatcagca ggtagccgag ggtcacatcg ggggagacat ccagacccac cggacccgcg
      781 ccgttgatca ggccgacgaa cgagagcacc gcaccggaga gcgcggcgac cgcggcgcgg
      841 tggaagtcgc ggtcgatgac aaaggccgca agcgcgccca gcaccatgcc ggcaagcacg
      901 gcccctccac cgaacagcgc catcccgtgg tagatgtcgc ccgtgccgag cttgtcgagc
      961 cccagctttg ccgcatcggt gccggcggcc ccgagcgcgt cgtccaccag cgtcttggcc
     1021 cataacgcaa tgttggggag gagggccagc accaccgcgg gcgcatgccg cgccggggtc
     1081 gcctggaacg cctgggcgcc gatcacgagc ccgatgaaga gcaggatcgg cacgagggcc
     1141 tggatcggga tcagcgccag caggagcgcc acgagcccga ggaaggccac gagcgcgatc
     1201 acgatcccgg tcaccagcga atagccgatc cgccccccga ccgtcttcca cccgggatgc
     1261 ccgatgtaca ccgccggggg aaaggggctc cccaggaaag agccgacgac cgcgcccgtg
     1321 ccgtccgcga gcaggatcca gcggagattg tagctatcgc cggccgccgc ggcgctctcg
     1381 acgttgttca tcccctccgt gaagttgtag acgccgaggg ggatcgccgt cgcgagcagc
     1441 ggcccgatgt ccgccaatcc ccggaggacc tcccgccctg ggatcggcag gtgcacgccg
     1501 aactgcctga acgactcgcc gacggccgag gggacgagca cccccgacca ccccagcagc
     1561 acggccaccc atcccaccgc ggtcccgatc accaccgccg ccagcccgcc gggcacgcca
     1621 aacgggagct tgacgttcgc gacccagctc accaggatga tcgccagaga gataaaggcg
     1681 atccagggca cctcccacat ctggaaggcc ggacgcatcg ag
//
LOCUS       DASQGH010000570         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2423, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000570 DASQGH010000000
VERSION     DASQGH010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1717
                     /gene="tcuA"
                     /locus_tag="VEW91_11265"
     CDS             335..1717
                     /gene="tcuA"
                     /locus_tag="VEW91_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HYM92201.1"
                     /translation="MIVVGGGNAALCAALAARREGARVLLLERAPESMRGGNTRHTRN
                     IRCAHADGDPYLSGEYAEEEFLEDLAGVTGGPTNPDLARFTIRESRSLPGWMTEHGVR
                     WQQPLAGTLHLGRTNRFFLGGGKALVNAYYETARRMGVDVRCEACVDDLIIEDGCFRA
                     VALGRGPSREVVPGRAVVVAAGGFEANLEWLKRYWGEAAGNFVVRGTPYNDGTVLAAL
                     LARGAMQVGDPRGLHAIAVDARAPRYDGGIVTRLDAIPFGIVVNRLGRRFYDEGEDLW
                     PKRYAIWGRLIAEQPGQVAYCILDAKTIDCFLPPMYKPYRADALEPLAAELELDPGAL
                     AETVRTYNRATTGNTHFRLDLLDGTRTRDLTPPKSNWALPIDRPPFYGLPLRPGITFT
                     YLGVAVDATARVLDRSGRPLQNVYAAGEIMAGNILSRGYLGGFGLTIGTVFGRVGGRE
                     AAKAAMYARA"
     gene            1704..2822
                     /gene="tcuB"
                     /locus_tag="VEW91_11270"
     CDS             1704..2822
                     /gene="tcuB"
                     /locus_tag="VEW91_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HYM92202.1"
                     /translation="MRGRDLFEEAERQLGICNACRYCEGYCAVFPALELRRAFAEGDI
                     AFLAHLCHDCRACYYACMYTPPHEFGVNIPQVLSAVRRASYRRYSWPELLSRAFADHR
                     LGVALAAAAAALVAALAWGFAGPARLFAAHRGPGAFYRVIPYWAMLVPGLALSFYVVA
                     VWLAGGARFWRETGGVLRQPGGLAALAGAAGEAVTLRWLRGGGPECYYPGARASSHRR
                     ILHALVFYGFVAALASTTLAAIYQDLLHRLPPYALTSAPVVLGSLGGVAMVAGSVTLI
                     LLKLRSDPAPADTGGTALDYVFLAVLGLAALTGLLTLVLRDTRAMGSLLTVHLGLVAA
                     LFVTAPYGKFVHFLYRYLALVKNGLEQRRRRERPASLP"
     gene            complement(2826..3017)
                     /locus_tag="VEW91_11275"
     CDS             complement(2826..3017)
                     /locus_tag="VEW91_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92203.1"
                     /translation="MAEVSVCEKCGLKAVRHELERQGVIMKFFDSCYWGEVDPGATPP
                     PGPLRETDVPTPGTKPREK"
BASE COUNT          431 a         1110 c         1024 g          485 t
ORIGIN      
        1 attttggtcc ggaactcacg cacgtcggtg cccggtacag cgccgacaaa ctcatggccc
       61 tgattcgcaa tcctgtctcg gtcaatccgc aggccagcat gcccgcgttc gacaagctct
      121 ccgacgaaga cctgaaagcg ctggtcacct acctcctgac cctcaaatag cacgttcatc
      181 gatcaccgcc ctatcgccgc gcccgacgcc cctgccaccc gagtcatttc cacggagcaa
      241 ccaaccccat ggccaggatg ccgggccatc cgagaggaac tgaaccgtgc aaggggtgtc
      301 ccaaggagga gtcgggccca cggaaaccta ccaggtgatc gttgtcggcg gcggcaacgc
      361 ggcgctgtgc gccgcactgg cggcccggcg cgagggagcg cgggtcctgc tactggagcg
      421 ggcccctgaa tcgatgcggg gcgggaacac ccgccacacc cggaacatcc gctgcgccca
      481 cgccgacggc gacccctatc tctcgggcga gtatgccgag gaggagtttc tcgaggatct
      541 cgcgggggtg accggggggc ccacgaaccc cgacctcgcc cggttcacga tccgcgaatc
      601 gcgaagcctc ccggggtgga tgaccgagca cggggtccgg tggcaacagc cgctggccgg
      661 caccctccac ctgggacgca ccaaccggtt cttcctcggc ggaggaaagg cgctggtcaa
      721 cgcgtactac gagaccgccc gccgcatggg agtggacgtc cgctgcgagg cctgcgtcga
      781 cgatctcatc atcgaggacg gctgcttccg ggcggtcgcc ctcgggcggg ggcccagccg
      841 cgaggtggtg ccgggccgcg ccgtggtggt ggcggccggg ggcttcgagg cgaacctcga
      901 gtggctcaag cgctactggg gggaggcggc cggcaacttc gtcgtccgcg gcacccccta
      961 caacgatggg accgtgctcg ccgccctgct cgccagggga gcgatgcagg tgggcgatcc
     1021 caggggcctc cacgccatcg ccgtggacgc gcgggctccc cgatacgacg gaggcatcgt
     1081 cacgcggctc gacgcgatcc ccttcgggat cgtggtcaac cggctggggc ggcgcttcta
     1141 cgacgaaggc gaagacctct ggcccaagcg ctacgcgatc tggggacggc tgatcgccga
     1201 gcagccaggc caggtggcct actgcatcct ggacgccaag acgatcgact gttttctccc
     1261 gccgatgtac aagccgtatc gggccgacgc gctggagccc ctggccgccg agctcgagct
     1321 ggatccgggc gcgctcgcgg agacggtccg cacgtacaac cgggccacga ccgggaacac
     1381 ccactttcgg ctggatctcc tggacggcac ccgaacccgc gaccttacgc cgccaaagag
     1441 caactgggcg ctccccatcg accgcccccc gttctacggt ctgccgctgc ggccgggcat
     1501 caccttcacc tacctcggcg tggctgtcga cgcgacggcg agggtgctgg accggagcgg
     1561 ccggcccctc cagaacgtgt acgcggccgg ggagatcatg gccggcaata tcctctcccg
     1621 ggggtacctg ggcggcttcg gcctcaccat cggcaccgtg tttggccggg tcgggggacg
     1681 ggaggcggcg aaggcggcga tgtatgcgcg ggcgtgacct gttcgaggaa gccgagcgcc
     1741 agctcgggat ctgcaatgcc tgccgctatt gcgagggcta ctgcgcggtc ttcccggccc
     1801 tcgagctgcg ccgcgccttc gccgagggcg acatcgcctt cctcgcccac ctgtgccacg
     1861 actgccgcgc ctgttactac gcctgcatgt atacgccgcc gcacgagttt ggggtcaaca
     1921 tcccccaggt cctgtccgcg gtgcggcggg cgagctaccg gcgctacagc tggccggagt
     1981 tgctgtcccg ggccttcgcc gaccaccggc tcggtgtagc cctcgcagcg gccgcggcgg
     2041 cgctcgtcgc ggcgctggcg tgggggttcg ccggcccggc gcgcctgttt gccgcccacc
     2101 gcgggccggg cgccttctat cgggtcatcc cctactgggc gatgctcgtc cccggactgg
     2161 ccctgtcctt ctacgtcgtg gcggtgtggc tggcaggcgg cgcgcggttc tggcgggaga
     2221 ccgggggcgt gctgcgccag ccgggcggcc tggcggccct ggccggggct gcaggcgagg
     2281 cggtgaccct gcgctggctt cggggcggcg ggccggagtg ctactacccg ggcgcccgcg
     2341 cgtcgtccca ccgccgcatc cttcacgcgc tcgtcttcta cggcttcgtg gctgccctgg
     2401 cgtccaccac gctcgccgcc atctaccagg atctgctgca ccggctgccg ccctatgccc
     2461 tcaccagcgc gccggtcgtc ctggggagcc tggggggtgt ggccatggtg gccgggagcg
     2521 tgaccctgat ccttctgaaa ttgcggagcg atcccgcacc agccgacacc ggtgggacgg
     2581 ccctggacta cgtgttcctg gctgtgctcg gtctcgccgc gctcaccggc ctactcaccc
     2641 tggtgctccg cgacacacgg gcgatgggga gcctgttgac cgtgcacctg ggcctcgtcg
     2701 ccgccctgtt tgtgaccgcg ccctacggaa agtttgtcca cttcctctac cgctacctcg
     2761 cgcttgtcaa gaacggactc gagcaacgcc ggcggcgcga acgcccggca agtctcccct
     2821 gaccctcact tctcgcgcgg cttcgtcccg ggtgtcggca cgtccgtctc ccgaagtggg
     2881 ccggggggcg gggtcgcgcc ggggtcgacc tctccccagt aacaagagtc gaagaacttc
     2941 atgatcacgc cctgccgctc cagttcgtgc cggaccgcct tcaagccgca cttctcacac
     3001 accgacactt cagccatgca tcccaccctt tggcccggac gactcctacc
//
LOCUS       DASQGH010000571         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5260, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000571 DASQGH010000000
VERSION     DASQGH010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5260"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1220
                     /locus_tag="VEW91_11280"
     CDS             132..1220
                     /locus_tag="VEW91_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HYM92204.1"
                     /translation="MFARKEAVTKEAVLEALRDVLDPELHRSIVELNMVKDVTVHDGE
                     VRIDALLTISGCPLKETITGSIKAKVEALAGVDRVDVHLGVMSQEQRQALIGQLRPGP
                     QRQSPLLSPTSTTRVLVVASGKGGVGKSTVTTNLAVALSRRKRIVGIADADVYGFSIP
                     HMLGVKGRPTVIDQMIIPLEREGIRVISIGFMVDENEAVIWRGPMLHKTITTFLGEVY
                     WGDTQDLLIDLPPGTGDVSLSIAQTLPRAEMLIVTTPQPAAANVAYRAARMAEKVNMP
                     VVGVIENMSYYVSVPGAEPAYIFGQGGGARLAEMIGAPLLGQIPLDPAIREGGDRGQP
                     VALTHPDAPASQVFYQIADALLGGGKTR"
     gene            1217..2041
                     /locus_tag="VEW91_11285"
     CDS             1217..2041
                     /locus_tag="VEW91_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HYM92205.1"
                     /translation="MTPAVVNDEIERFVRPQTFPLGIRMAKPGEPLPEKIRRPAQDMG
                     IKVAICQTFSIARRYGWALAVGRDDLSCPLAKTAFGFEQVLPYYAEGNLACGMYAATP
                     EAGERTEAEVPKFAFGEYERIIVAPLGRATFEPHVVLVYANAAQVMRLVAAALYQRGG
                     RIHSSFAARLDCADAVIETIHTGRPQVILPCYGDRIYGQTEDHEMAFTVPWAGAEDLV
                     AGLQGTHQGGVRYPIPAWLRYTGEFPEKYRRLDALWEEGRPGSPRPAGGGGTGAKP"
     gene            2186..3931
                     /locus_tag="VEW91_11290"
     CDS             2186..3931
                     /locus_tag="VEW91_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYM92206.1"
                     /translation="MTIRLAGEAGQGVESGGAGFAKALSHGGLWLHAFSEYMSRIRGG
                     LNFFQIRVSDRPLWAHTEGVHILLAFSPEAVSEYGPHIVPAGALMFDDALKFDHDAMS
                     RRKVQLYSMPLTKIAQEVGGNKIMANTCGLGALAGVIEYPLEFVADVIKQNFARKGDA
                     VVQGNLRVAEEGYRVGRERYAASFEWKVSPLGERSDRMMINGNQALSVGAIAAGCRFM
                     AGYPMTPASSILEFIAGHARKYDIVVKQTEDEIAAICFAIGAGNAGARAMTATSGGGF
                     SLMVEALGLAGMAEVPVVIIEAQRPGPSTGMPTKTEQGDLLFALFASQGEFPRIVLAP
                     GTQEECFHTAVRAFNLAEKWQCPVIIMTDSYLTTMMRLVPPAEFPIERIPIDRGELLT
                     PEQLDRLSEPYLRYRETPSGISPRALPGHPRGVHQACSDEHDEYGHFEDEDALNRMKM
                     AGKRWRKFRNIVEDLRGPAIYGPEGAAITLMGWGSSYGAMRETVDILNARGTRVNLMH
                     FVDIWPFPEAKAGPLIEAARVLVAVEGNQSGQFAHLVRAVTGRRADHHVLRWDGRPLS
                     PEYIIRHLEEAKVHA"
     gene            3924..>4230
                     /locus_tag="VEW91_11295"
     CDS             3924..>4230
                     /locus_tag="VEW91_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM92207.1"
                     /translation="MLDVKTYNNPIKVTWCPGCGDYGILNAVKSALAQLEIHPHEVMF
                     FSGIGCGSKLPDYMNANAFTTIHGRSLPVAMGTKLANHDLHVICITGDGDGYGIGGNH
                     "
BASE COUNT          719 a         1345 c         1518 g          648 t
ORIGIN      
        1 gcttgggggg cgggtgctat cctaggtgct gaaagtgata attactctca gcagtcatct
       61 tcacccccgc agctgagagc ctgacggccg ggtcccagtc tcaacgcccc gccgggcgca
      121 aggaggaaga cgtgtttgcg cgcaaagaag ccgtcaccaa agaagcggta ctcgaggcgc
      181 tccgcgacgt cctggacccc gagcttcacc gcagcattgt cgagctcaac atggtgaagg
      241 acgtcacggt gcacgatggg gaggtccgga tcgacgcgct gctgacgatc agcggctgcc
      301 cgctcaagga gacgatcacc ggctcgatca aagccaaggt cgaggccctg gccggcgtgg
      361 atcgcgtgga cgtgcacctc ggcgtgatgt cgcaagagca gcgccaggcg ctcatcggcc
      421 agttgcggcc cgggccgcag aggcagtccc cgctgctgtc gccgacgtcg acgacgcgcg
      481 tgctcgtcgt cgcgagcggg aagggtgggg tcggcaagtc cacggtcacc acgaacctgg
      541 ccgtggccct ctcgaggcgc aagcggatcg tcggcatcgc ggatgccgac gtctacgggt
      601 tcagcatccc ccacatgctg ggggtcaagg gccggcccac ggtcatcgat cagatgatca
      661 tcccgctgga gcgcgagggg attcgcgtca tctccatcgg gttcatggtg gacgagaacg
      721 aggcggtcat ctggcgcggc ccgatgctcc acaagacgat caccaccttc ctgggcgagg
      781 tttactgggg ggacacccag gacctcctga tcgacctgcc gccgggcacc ggggatgtct
      841 cgttgagcat cgcgcagacg ctcccgaggg cggagatgct gatcgtgacc acgccccagc
      901 cggccgcggc caacgtcgcc taccgcgccg cccggatggc ggaaaaggtc aacatgccgg
      961 tcgtgggcgt gatcgagaac atgtcgtact acgtgagcgt cccgggggcc gagccggcgt
     1021 acatcttcgg ccagggcggc ggcgcccggc tcgcggagat gatcggcgcg ccgctcttgg
     1081 ggcagatccc gctcgacccg gcgattcgcg aaggcggcga ccgcgggcaa cccgtggcgc
     1141 tgacccaccc tgacgcgccg gcctcgcagg tgttctacca gatcgcggac gcgctccttg
     1201 gaggcggcaa aacacgttga cccccgcggt tgtcaacgac gagatcgagc gattcgtccg
     1261 gccccagacc tttcccctgg gcatccggat ggcgaagccc ggggagcccc tccctgagaa
     1321 gatccgtcgg cccgcccagg acatggggat caaggtcgcg atctgccaga cgttttcgat
     1381 tgcccggcgg tacgggtggg ccctcgcggt cggccgggac gatctcagct gtcccctggc
     1441 caagacggcg ttcgggtttg agcaggtcct tccgtactac gccgagggga atctggcctg
     1501 cgggatgtat gccgcgaccc ccgaggccgg tgagcgaacc gaggcggagg tgccaaagtt
     1561 cgcgttcggg gagtacgagc ggatcattgt cgccccgctc gggcgcgcga cctttgagcc
     1621 ccacgtcgtg ctggtgtacg ccaacgccgc ccaggtgatg cggctggtcg cggcggcgct
     1681 gtatcagcgg gggggccgga tccacagcag ttttgccgcc cggctggact gcgccgatgc
     1741 ggtcatcgag acgatccaca cggggcgccc ccaagtgatc ctcccgtgct acggggaccg
     1801 gatctacggg cagaccgaag accacgagat ggcgttcacc gtgccgtggg ccggggcgga
     1861 ggatctcgtc gcggggctgc aggggacgca tcagggcggg gtgcggtacc cgattcccgc
     1921 gtggctccgg tacaccggcg agtttcccga gaagtaccgg cggctggacg cgctgtggga
     1981 agaaggccgc ccgggatccc cccggcccgc agggggcgga gggaccggcg cgaagccgta
     2041 gggggcataa agcgtgatgg ggacgcgttg atggagtgcg aattcgggct gcctgcgtgg
     2101 ggggcgctcc acgagggcgc agtgacgcgc gggctgccca gccttcaccg ggggtgagca
     2161 aggcgtggcg aaacggatca acgacatgac gatcaggttg gcgggcgaag cgggccaggg
     2221 tgtcgagtcc ggcggggccg ggtttgcaaa ggccctgagt cacggcgggc tctggctcca
     2281 cgcgttctcc gagtacatgt ctcggatccg cggcgggctc aacttcttcc agatcagggt
     2341 ctcggaccgc ccgctgtggg cgcataccga gggggtccac atcctgctcg ccttcagccc
     2401 ggaggccgtg agcgagtacg gaccccacat cgtcccggcc ggggcgctca tgttcgatga
     2461 tgcgctgaag ttcgaccacg atgcgatgtc ccgccgcaag gtgcagctgt acagcatgcc
     2521 cttgacgaag atcgcccagg aggtcggcgg caacaagatc atggccaaca cctgcggcct
     2581 cggggcgctc gccggcgtca tcgagtatcc gttagagttc gtggccgacg tcatcaagca
     2641 gaactttgcg cggaaaggcg atgcggtcgt gcagggcaac ctccgggtgg ccgaggaggg
     2701 ctaccgggtc gggcgcgagc gctacgccgc ctccttcgaa tggaaggtct cgccgctcgg
     2761 ggagcgatcg gaccggatga tgatcaacgg caaccaggcg ctgtcggtcg gtgcgatcgc
     2821 ggcggggtgc cggttcatgg cggggtaccc gatgacgccg gccagttcga tcctggaatt
     2881 tatcgccggc catgccagga agtacgacat cgtggtcaag cagaccgagg acgagattgc
     2941 ggcgatctgc tttgcgatcg gggcggggaa cgccggcgcc cgcgcgatga ccgcaacgag
     3001 cgggggcggg ttctcgctca tggtggaggc cctcgggctg gcgggcatgg cggaggtccc
     3061 cgtggtgatc atcgaagcgc agcggcccgg tccgtcgacc gggatgccca ccaagaccga
     3121 gcagggggat ctgctcttcg cgctcttcgc gagccagggg gagttcccgc ggatcgtcct
     3181 ggccccgggc acccaggagg agtgcttcca taccgccgtc cgggcgttca acctggcgga
     3241 gaagtggcag tgcccggtca tcatcatgac cgattcctac ctgaccacga tgatgcggct
     3301 cgtgcccccg gcggagttcc cgatcgaacg gatcccgatc gaccggggcg agctcctgac
     3361 cccggagcag ctcgaccgct tgagcgagcc ctacctgcgg taccgggaga ccccgagcgg
     3421 gatctccccg cgggcgctgc cggggcaccc ccggggggtc caccaggcct gcagcgatga
     3481 gcacgacgag tacggtcatt tcgaggacga agacgccctg aatcggatga agatggcggg
     3541 gaagcgctgg cggaagttcc ggaacatcgt cgaggatctg cggggcccgg cgatctacgg
     3601 cccggaaggc gccgcgatca ccctgatggg gtgggggagc tcgtacgggg cgatgcggga
     3661 gacggtcgac atcctcaacg cccggggcac gcgggtgaac ctcatgcact tcgtggacat
     3721 ctggccgttc ccggaagcca aggcgggccc gctcatcgag gcggcccgcg tcctggtggc
     3781 ggtggaaggc aaccagagcg gtcagttcgc ccacctggtg cgtgcggtga ccgggcggcg
     3841 cgcggatcac catgtcctgc gctgggacgg acggccgctc agccccgagt acatcatccg
     3901 gcacctcgag gaggccaagg tccatgcttg acgtcaagac ctacaacaat cccatcaaag
     3961 tcacctggtg ccccgggtgc ggggactacg ggatcctcaa cgcggtgaag agcgccctcg
     4021 cacaactgga gatccacccc cacgaggtga tgttcttctc ggggatcggg tgcggcagca
     4081 agctgccgga ctacatgaac gccaacgcgt ttacgacgat tcacggacgg tcgttgccgg
     4141 tcgccatggg gaccaagctc gccaaccacg acctgcacgt gatctgcatc accggggacg
     4201 gggatgggta cgggatcgga gggaatcact
//
LOCUS       DASQGH010000572         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_30924, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000572 DASQGH010000000
VERSION     DASQGH010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30924"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..559
                     /gene="moaC"
                     /locus_tag="VEW91_11300"
     CDS             23..559
                     /gene="moaC"
                     /locus_tag="VEW91_11300"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYM92208.1"
                     /translation="MSTERSASRVTHLDRHGRARMVDVGRKRETRRAAVARGEVAMAP
                     ATLRLITAGAARKGDVLGVAQVAGIAAAKRTWELIPLCHPIPVTGISVRLTPDPQASR
                     VQIEARVRTVGRTGAEMEALVAVAVAGLTVYDMLKAAERGIRIEHLRVVRKSGGKSGT
                     YVRGDPPARRSPRAIRRR"
     gene            556..1080
                     /locus_tag="VEW91_11305"
     CDS             556..1080
                     /locus_tag="VEW91_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HYM92209.1"
                     /translation="MSEEQRLRGVRCGILTASERVSRGQVKNESGQYLVRTVGAEGGE
                     VVAHVVVPDDLEMIAKTLADMADTLGVDLVLTTGGSGVAPRDVTPEATLRVIERQIPG
                     IPDAARVATAAASPLAMLSRAIAGIRGRTLIVNLPGSPRGVQEWMAVILPVLRHAVEL
                     LRETPMEWGREHKP"
     gene            1085..3040
                     /locus_tag="VEW91_11310"
     CDS             1085..3040
                     /locus_tag="VEW91_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HYM92210.1"
                     /translation="MPLLLATELTKSYGTLPVLAGATLALEPGEKAALVGRNGAGKTT
                     LLRILAGLEAPDGGTRALSGGATVGYLAQDPTVDESRTLWDEAAAPFAHLALAERRLA
                     ELEARLAAPELHRDDARLSGVLEDYGRLREQFETGGGFTYEAEIRRTLFGLEFREEQF
                     RQPLASMSGGQRSRAALARLLLSAPDLVLLDEPTNHLDLDGQEWLEAFLTQYGGAVLL
                     VSHDRRLLDVVTTRTLELEEGRLESYPGNYSYYVTERAARRARQQEAFERQQEEVESL
                     KAYIRRYHAGQKSRQAKSREKRLARIQPIAAPRLHRTASIRLETPRRTPQVVLRLRGV
                     TKRFGPLEVLRGVTCEVRRGEKIGLIGPNGAGKTTLLRIIARTESPTSGAVEPAPGVR
                     IGYFSQLSDETLDLDQTVLDEVLGARPVTPAQVRGLLGRFLISGDDVYKKIAQLSGGE
                     RRRVALAKLVLDRPDILLLDEPTTHLDLTTLEALEAALRGFPGAMLFVSHDRTFLDRL
                     ADRLLVLGDGVVQFFAGPYHTYRAARAGGPPAATRHGPVAADARLGEARGRGDRAEGG
                     TRKATRSSRPAFAEAHPGAPVAEEIMAKITALERELQDLSRIMGDPELYRDAVRARQT
                     VQRYEELTAALESLYAKLEGATGAPHA"
     gene            3033..3419
                     /locus_tag="VEW91_11315"
     CDS             3033..3419
                     /locus_tag="VEW91_11315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYM92211.1"
                     /translation="MREAGGCFACGQDNPIGLRLRFTAEGDGVRAEFIPGPQYQGYEG
                     VLHGGIVTAALDDAMANLFHLRGRESVTARLEIRFRREAPVGQRLVVTARLTGERRRL
                     FTAEAVLTLPDGTRLAEAKGTFVREA"
     gene            3454..3915
                     /gene="smpB"
                     /locus_tag="VEW91_11320"
     CDS             3454..3915
                     /gene="smpB"
                     /locus_tag="VEW91_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HYM92212.1"
                     /translation="MKPVAINRRARHEFSIEETHEAGLVLTGTEVKSLREGRVSLADA
                     FARVDGGEVWLHHLHISPYAQGNIHNHDPLRTRKLLLHRREILRLRSKTDRKGYTLVP
                     LRLYFRHGVAKVELAVARGRHLYDRRERIAEREAERRIARNIGARRPRQQP"
     gene            complement(3965..4222)
                     /locus_tag="VEW91_11325"
                     /pseudo
     CDS             complement(3965..4222)
                     /locus_tag="VEW91_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013394.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
BASE COUNT          692 a         1489 c         1606 g          587 t
ORIGIN      
        1 tactggcggg ggtacgagaa gcgtgtccac tgagcggtcc gcgtcgcggg tgacccacct
       61 agaccgacac gggcgcgccc gcatggtgga cgtgggccgg aagcgcgaga cgcggcgcgc
      121 ggccgtggcc cggggcgagg tggcgatggc gccggcgacg ctccggctga tcaccgcggg
      181 ggcggcccgg aagggcgacg tgctcggggt ggcccaggtc gccggcatcg ccgctgcgaa
      241 gcggacgtgg gagctgatcc cgctgtgcca tccgattccc gtgaccggga tctcggtgcg
      301 tctcaccccc gatccccagg cctcccgggt gcagatcgaa gcgcgcgtgc ggacggtggg
      361 ccgtaccggt gcggagatgg aggccctcgt cgccgtcgcc gtggcgggcc tcaccgtcta
      421 cgacatgctc aaggcggccg agcgcggcat ccggatcgag cacctccggg tcgtgcggaa
      481 gtccgggggg aaatccggca cgtacgtccg cggcgatccc cccgcgcggc gatccccccg
      541 cgcgatccgc cggcgatgag cgaagagcag cggctgcggg gcgtgcgctg cggcatcctg
      601 acggcgagcg agcgcgtcag ccgcggccag gtgaagaacg agagcggaca gtatctcgtc
      661 cggacggtcg gggcggaggg gggcgaggtc gtcgcccacg tcgtggtccc ggatgatctg
      721 gagatgatcg cgaaaacgct cgcggacatg gccgataccc tgggcgtgga tctcgtgctg
      781 accaccggcg gcagcggggt ggcgccccgg gacgtgaccc cggaggccac cctgcgggtg
      841 atcgagcgcc aaatccccgg cattcccgac gcggcccggg tggcgaccgc ggcggcgtcg
      901 ccgctcgcga tgctctcgcg cgcaatcgcc gggatccgcg gccgcacgct catcgtgaac
      961 ctgcccggga gcccccgcgg ggtccaggaa tggatggccg tgatcctgcc ggtgctccgg
     1021 cacgcggtgg aactgctgcg cgagaccccg atggagtggg gccgcgagca caaaccgtag
     1081 gacgatgccg ctcctgctcg ccacggaact gacgaagtcg tacggaaccc tcccggtgct
     1141 cgccggggcg accctcgccc tcgagcccgg ggagaaagcg gcgctcgtcg gcagaaacgg
     1201 cgccgggaag accaccctcc tccggatcct cgcggggctc gaggcacccg acggcggcac
     1261 gcgggcgctc tcaggcgggg cgaccgttgg gtacctcgcg caagatccca ccgtggacga
     1321 gtcgcgcacg ttatgggatg aagcggccgc gccgttcgcc cacctcgcgc tggccgagcg
     1381 gcgcctggcg gagctcgagg cgcggctcgc ggcgcccgag ttgcatcgcg acgatgcacg
     1441 gctctcgggg gtgctcgagg actacggccg cctccgggag cagttcgaga ccgggggagg
     1501 gttcacatac gaggccgaga tccgccgaac gctcttcggc ctggagttcc gggaagagca
     1561 gtttcgccag cccctggcct cgatgagcgg cggccagcga tcgcgggccg cgctggcgcg
     1621 cctgctgctc tcggcgcccg accttgtgct gctcgacgag ccgaccaacc atctggacct
     1681 ggacggccag gagtggctcg aggccttcct cacgcagtat gggggagcgg tgctcctggt
     1741 gagtcacgat cgccggctcc tcgacgtggt cacgacccgc acactggaac tcgaggaggg
     1801 ccggctcgag tcctaccccg gcaactactc ctactacgtc accgaacggg ctgcgcggcg
     1861 cgcccggcag caggaggcgt tcgagcggca gcaggaggag gtcgagagcc tcaaggctta
     1921 cattcgccgg taccacgccg gccagaaaag ccgacaggcg aagtcgcggg agaagcggct
     1981 cgccaggatc cagccgatcg ccgccccgcg gctccaccgg accgcatcga tccggctgga
     2041 gaccccgcgt cgcacccccc aggtggtcct ccggctgcgg ggggtcacca agcgattcgg
     2101 acccctcgag gtgctgcggg gagtgacctg cgaggtgcgc cgcggcgaga agatcgggtt
     2161 gatcgggccg aacggcgcgg ggaagaccac gctcctcagg atcattgcgc ggaccgaatc
     2221 ccccacgtcg ggggcggtgg agcctgcccc gggggtgcgc atcggctatt tctcgcagct
     2281 gtccgacgag acgctcgatc tggaccagac ggtgctggac gaggtcctcg gcgcccggcc
     2341 ggtgacgccg gcccaggtgc gcgggctgtt ggggcggttc ctcatttcgg gggacgatgt
     2401 ctacaagaag atcgcccaac tgagcggcgg ggagcggcgc cgggtggccc tcgccaaact
     2461 cgtgctcgat cggccggaca tcctgctgct cgacgagccg accacccacc tcgatctcac
     2521 caccctggaa gccctggagg cagcgctccg cgggttcccc ggggccatgc tgtttgtgtc
     2581 tcacgaccgc accttccttg accggctcgc ggaccggctg ctggtcctcg gcgatggggt
     2641 ggtgcagttc ttcgcgggcc cgtaccacac gtaccgggct gcgcgtgccg gagggccgcc
     2701 tgcggccacc cggcacggac cggttgcggc ggacgcgcga ctgggggagg cccgcgggag
     2761 gggagacagg gccgaggggg ggacccggaa agccacccgt tcgtcccggc cggcctttgc
     2821 ggaggcccat cccggagctc cggtcgcgga ggagatcatg gcgaagatca cggccctgga
     2881 gcgggagttg caggacctca gccgcatcat gggcgatccc gagttgtacc gcgatgccgt
     2941 ccgcgcccgg cagaccgtgc agcggtacga ggaactgaca gccgcgcttg agtcgctcta
     3001 cgccaaactc gaaggcgcca ccggggcccc ccatgcgtga ggccggcggc tgcttcgcct
     3061 gcgggcagga caatcccatt gggctacggc tccgattcac cgcggaaggc gacggcgtgc
     3121 gcgctgaatt catcccggga ccgcagtacc aggggtacga aggcgtcctc cacggcggca
     3181 tcgtgaccgc ggcgctggat gacgcgatgg ccaacctgtt ccacctgcgc ggccgcgagt
     3241 cggtgacggc acggctggag atccgctttc gccgggaggc gccggtcggg cagcggctcg
     3301 tcgtgaccgc gcggctcacc ggcgagcgga ggcgcctctt taccgccgag gcggtgctca
     3361 ccctgccgga cggcacccgg ctcgccgagg ccaagggaac gttcgtccga gaggcgtagg
     3421 gacgtggccc gcggaacggt cgaggagcag gggatcaagc cggtggcgat caaccgccgg
     3481 gcccggcacg agttctcgat cgaggagacc cacgaggcgg ggctggtcct caccggcacc
     3541 gaggtcaaat cgcttcgcga gggccgcgtc agcctcgcgg acgcctttgc ccgggtcgac
     3601 gggggggagg tgtggctcca ccacctgcac atcagtccgt atgcgcaggg gaacatccac
     3661 aatcacgacc cattgcggac ccgcaagctg ctgctgcacc gccgggagat cctgcgcctc
     3721 cggtccaaga cggaccgcaa aggctacacg ctcgtccccc ttcgattgta cttccgccat
     3781 ggggtggcca aggtcgagct ggccgtcgcc cgtgggcggc atctctacga cagacgcgaa
     3841 cggatcgccg agcgggaggc cgagcggcgc atcgcccgga acatcggcgc gcgcaggcct
     3901 cggcaacagc cctgatccgc atccgggttg cccaccgggt gccatccgtc ccgaggccct
     3961 gcgctcaata aattccgggg acgagccggg ggatgtgctc agcgtagcgg tcgtagtccg
     4021 cgccaaacag ggtgcggagg tgggcctcct ccaagtggat ccgccgcagg agcacgatca
     4081 gccaggcaca cgccagcccc caagccacca cgctggccag acccagcgcg agcccggcgc
     4141 gaacggcgag gattcccgcg tagcgcgggt gccgcacaaa ctggaaagga ccgtaggtca
     4201 tcaatcccgg cagtgaggct aaccgcgtga gccaatggta taatagtact tgaagtgggt
     4261 ttcaggacac atgacggagt atgggggcga caggtctcga ctgagcaggc agaagcaggg
     4321 tggcgcgccg aggtggtcca ggccctcgtt aaacaactgg acggcgcata cacg
//
LOCUS       DASQGH010000573         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10856, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000573 DASQGH010000000
VERSION     DASQGH010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10856"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VEW91_11330"
     CDS             <1..377
                     /locus_tag="VEW91_11330"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92213.1"
                     /translation="VFDLMDAVGGRQAERALRLLQVVLAAEAPVKVLFMLGDQIRMLL
                     RTKTLLERHPPGRRPPADAIRDALGTRAFLYDRYRAQVAAFGRMDVARLLGLVAETDT
                     EIKTGMKPPRLALETLIVGLCV"
     gene            complement(407..673)
                     /gene="rpsT"
                     /locus_tag="VEW91_11335"
     CDS             complement(407..673)
                     /gene="rpsT"
                     /locus_tag="VEW91_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYM92214.1"
                     /translation="MAKRSKSGLKRMRQAVKRTARNQAVRSTIKTLVKQARLASAAAA
                     PEVQAAIRVLDAAARQRIIHPNAAARSKSRLMRHLARQHPATPS"
     gene            790..>1985
                     /gene="murJ"
                     /locus_tag="VEW91_11340"
     CDS             790..>1985
                     /gene="murJ"
                     /locus_tag="VEW91_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYM92215.1"
                     /translation="MSTGRTELASARLTRAAGVIAVGTVASRGLGLIRDVFIAAFFGA
                     TSAKSAFVIAYAIPFFVQRLFLAGILSIVFIPAITEVLTRNDADETRRVVTSTLNLVL
                     LIGCGLTVFGILAAPLLVPVAAPGYLRTNPAVLTSAIGLTRIMFVSMAFLALSGFVTG
                     YLNAHYRFAVPALAPLVFNLVIIAGVAWLGGRLGIRGVAVSFLLGWAAQFLVQLPAAR
                     QAGLRWGTSLDLHHPAIREMGRLAIPAMLGLAVIEINANVGRFFTSFLPPRPAVDYVA
                     VLDYAFTVNQAPVSIFALSLATALFPTMARRAAEGPALLRETTSLGLRGVLFTMVPVM
                     AAMLALSEPLVRVVFQRGAFAPQATHAVALGLIGFAVGSVPYAAYYIVTRTFYAMHDT
                     RTPVRIG"
BASE COUNT          246 a          702 c          685 g          352 t
ORIGIN      
        1 ccgtcttcga tctgatggat gccgtgggtg gtcggcaggc cgagcgggcg ctgaggctgc
       61 tgcaggtcgt gctggcggcg gaggcgccgg tgaaggtcct cttcatgctg ggggaccaga
      121 ttcgaatgct gctgaggacc aagacgctgc tggagcgcca ccctcccgga cgccggcccc
      181 ccgccgacgc gatccgggac gcgctgggga ccagggcatt tctctacgat aggtaccgcg
      241 cccaggtggc cgcatttggc cgcatggacg tcgcgcgcct cctggggctg gtggccgaga
      301 cggacaccga gatcaagacg gggatgaagc cgccccggct cgccctcgaa acgctgattg
      361 tcgggctctg cgtctagcgg gccccgcgcg gatccgccgc gcagcgctag gagggagtcg
      421 cggggtgctg ccgggccaga tgccgcatca ggcgggactt gctccgggcc gcggcgttgg
      481 ggtgaatgat ccgctggcgc gccgccgcat caagcacccg gatcgcggcc tggacctccg
      541 gcgcggcggc ggccgacgcc agacgcgcct gcttgaccag cgtcttgatc gtggaccgga
      601 ccgcctggtt ccgggccgtc cgcttgacgg cctgacgcat ccgcttcagc ccggatttcg
      661 agcgcttggc caatcgggca cctccgtgct cctgggggat tcaatgacgc gacgcccatt
      721 gtagcacacg cgtccgcgcg ccagcaacac ggaaggcccc gcccgcgcct gtgctataat
      781 ggcgggcaga tgtcgaccgg aaggaccgag ctggcctccg cgcggctcac ccgagcggcc
      841 ggcgtgatcg ctgtcggcac cgtcgccagc cgagggctcg gactgatccg ggatgtgttc
      901 atcgcggcct tcttcggcgc cacctccgcc aagtccgcgt tcgtcatcgc gtacgcgatt
      961 ccatttttcg tgcagcggct gttcctggcc ggcattctca gcatcgtgtt cattcccgcg
     1021 atcaccgagg tcctcacccg gaacgacgcg gacgagacgc ggcgcgtcgt gaccagcacc
     1081 ctgaacctgg tgctgctcat cggctgcggc ctgacggtgt tcgggatcct ggccgcgccg
     1141 ttgctcgtgc cggtggcggc gcccggctac ctccggacga acccggcggt gctcacctcg
     1201 gcgatcggcc tcacgcgcat catgttcgtc tcgatggcgt tcctggccct ctccgggttc
     1261 gtcaccgggt atctgaacgc gcactatcgc tttgcggtgc ccgcgctggc cccgctggtc
     1321 ttcaacctgg tcatcatcgc cggcgtggcc tggctcggcg ggcggctcgg gatccgcggc
     1381 gtggccgtga gcttcctcct ggggtgggcg gcacagttcc tcgtccagct gccggccgcc
     1441 cggcaggccg ggttgcggtg gggcacgtcc ctggatcttc accaccccgc gatccgcgag
     1501 atgggccggc tggcgatccc cgcgatgctc gggctcgcgg tgatcgagat caatgccaac
     1561 gtcggccgct tcttcacctc gtttctaccg ccccggccgg cggtcgacta cgtggcggtc
     1621 ctcgattacg cgttcaccgt caaccaggct ccggtcagca tctttgcgct ctccctggcc
     1681 acggcgctgt tccccacgat ggcccgccgg gccgccgagg gaccggcgct gctgcgggag
     1741 accacatcgc tcgggctccg gggggtcctc ttcaccatgg tcccggtgat ggccgccatg
     1801 ctggcgttga gcgagcccct ggttcgggtg gtgtttcagc ggggcgcatt cgcgccgcag
     1861 gccacccatg cggtggccct cggcctcatc gggttcgccg tcgggagcgt gccgtacgcg
     1921 gcctactaca tcgtcacccg gacgttctat gccatgcacg acacgcgaac gccggtgcgg
     1981 atcgg
//
LOCUS       DASQGH010000574         8413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_8247, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000574 DASQGH010000000
VERSION     DASQGH010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8247"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..657
                     /gene="def"
                     /locus_tag="VEW91_11345"
     CDS             160..657
                     /gene="def"
                     /locus_tag="VEW91_11345"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYM92216.1"
                     /translation="MEIITVDSPKASILRRRAQPVRTVTREIRGLIDQMFHALQGAHG
                     LGLAAPQVGVSRRLFVARLDDRQIALADPELLHQEGEQVATEACLSIPGLLGDVARAT
                     RVTVRGRNRHNRFVTIEATGLLARVLLHEIDHLNGILFTDRVQDPKTLRRVEGTSEVA
                     PATVA"
     gene            657..1595
                     /gene="fmt"
                     /locus_tag="VEW91_11350"
     CDS             657..1595
                     /gene="fmt"
                     /locus_tag="VEW91_11350"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYM92217.1"
                     /translation="MKLVFMGTPEFAVPSLEALLAASDLAAVVTRPDRPQGRGLRVTP
                     PPVAVAAAQYALDVLQPGTLRDPAFLARLRTLAPDLLVVVAFGRLIPPEVLALAPLGG
                     INLHPSMLPRYRGAAPIPRAIAAGETTTGVTVLHLSEELDAGDIILQRAVPIHPDDTT
                     ATLEGRLAQEGAGLLVEAVRLLETHQAPRTPQDPSRVTFAPKIAPEEAWVSWGDPAVK
                     IVNQVRAFDPWPVACTRRDGVLLKIWRAAALPGSGPEAPGTVVSAGREGLVVASGAGR
                     VQILEVQPAAGRRMPAADYLRGHPLAPGTMLGPPRG"
     gene            1630..2316
                     /locus_tag="VEW91_11355"
     CDS             1630..2316
                     /locus_tag="VEW91_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HYM92218.1"
                     /translation="MFWPLWDPTMIIVIPALLFALYAQFRVKSTFARWSEVRIANGRT
                     GAEIAREILRRNGLSEVEIDRTDGMLSDHYDPRTRTLSLSSDVHDGISVSSAGVAAHE
                     TGHAIQHARDYAPLALRSAMVPTVQFGSWLAWPLFFVGLIFHSGAMIQIGILIFTVAV
                     AFTIVTLPVEFDASARAMRALREEGLVTADELGGVRAVLTAAALTYVAAAAMAVLQLV
                     RMLLLARREE"
     gene            2345..3718
                     /gene="rsmB"
                     /locus_tag="VEW91_11360"
     CDS             2345..3718
                     /gene="rsmB"
                     /locus_tag="VEW91_11360"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HYM92219.1"
                     /translation="MAGPPGIPRTSREVALEVLHRVDTHQAWSTVLLRRMLDRAALRP
                     ADAALATELTYGTLRHRAELDWVLARVTRTPLDELPSHIRAVLRMGAYQLMFLDRIPP
                     RAACSESVDLAKRVGHPGTARLVNAVLRRIAAAPIAIPEDESTPDGIALRRSHPLWLV
                     RRWVDRFGVAGARALCAANNTTPPASVRLNTLRGSPAAVANAIAALGVHTMRSALLPE
                     GARIIEASAEARQAAYAAGWISPQDEGSMLVSRLVAPLPGETVLDACAGSGGKSLHLG
                     ALMGNQGRILACDVVPAKLDAIVRQARRLGVAIVTPRLLDATRLAAALSSDADRVLVD
                     APCSGLGVIRRRPEIRWRLQPEQLPALAARQREILDGAAAAVRPGGLLVFSVCTIEPD
                     EGETVASGFLAAHPEFSPEPISGWPPGSGEGPAPSPVPGRPGTAYLYPHVHDTDGFFV
                     AAFRRSR"
     gene            3715..4836
                     /gene="rlmN"
                     /locus_tag="VEW91_11365"
     CDS             3715..4836
                     /gene="rlmN"
                     /locus_tag="VEW91_11365"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYM92220.1"
                     /translation="MMAAPSPALLELRGLLRADLEALAARLGEPRYRGRQVARWLYVR
                     GAESVEAMTDLPQAFRDRLAEGARIGRLTVRQTQDAPDGSATKYLVALEDGQMIECVR
                     MRFDDGRRSACISTQAGCAMGCAFCATGLGGFARNLSAAEILGQFLLIRAHGRRRLTN
                     VVFMGMGEPLANYDATVRAVRILAAPYGMGIGMRHITVSTVGLVPQIRRLAKERLQMT
                     LAVSLHAPTDALRDRLVPVNRRYPLADLMAACREYVEATGRRLTFEYVLIDGVNDGPQ
                     EARALVRLLGGVRCHVNLIPLNPVAGIPFVRPPIPRVRAFAAALRTAGMAVTVRIERG
                     GEIQAACGQLRLADGLGRPSRWTPVAAAASSPAGRGGGR"
     gene            4833..5186
                     /locus_tag="VEW91_11370"
     CDS             4833..5186
                     /locus_tag="VEW91_11370"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYM92221.1"
                     /translation="MSGRARSDTRVYRPPAPGPRLRVLAGPPGEAGREFALDGPMVTI
                     GRRPDQRIVLSDPSVSRSHARVEVGAWGVAIVDLGSTNGTEVNGHRLRDGRAVLRGGD
                     RIRLGTVLLEFLAAR"
     gene            5183..6238
                     /locus_tag="VEW91_11375"
     CDS             5183..6238
                     /locus_tag="VEW91_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HYM92222.1"
                     /translation="MTAGKRLLPLASRAAGKSDIGRVREVNEDRILLLDPAGRGMAVY
                     AVADGLGGHAGGDVASTLAVETLRTELPALLAQRVPAREALPEALRRANAGIYAASQG
                     TDRAGMATTCTAVLVDGGEGVVAHVGDSRAYLLRGQAVRQLTTDHSLAGELARRGAPV
                     EAAHAQRHVLTRALGTARDVEIDCVAEPLRAGDVLVLTTDGLHNAVPPEEIGAVVRAA
                     ADLEDACRTLVGLANARGGVDNASAIVIRLTPWRGGRVMRLLAPIGLAAFLAGGVAVY
                     RLEHAYFLGVRDDRVAVMRGVPGRVLGVPLSGVVRVTQVPVTRIAPADRSRLARGIPT
                     ASPEEAESMLDGLLFHP"
     gene            6240..8147
                     /locus_tag="VEW91_11380"
     CDS             6240..8147
                     /locus_tag="VEW91_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYM92223.1"
                     /translation="MIGRSLGGKYEVTGPLGEGGMATVYRGRRLSDGRSVAIKILREQ
                     YAHDTEFVARFEREAQAVARLSHPHMVQVFDSGRDGDVHYIVMEFAEGEDLKTLLRRV
                     RVLSEQRAREIGAQVCEVLAYAHAEGIVHRDIKPQNILLTFDGQVKVTDFGIARALAS
                     AAITETGTVLGSVQYLSPEQARGLGVGQSTDLYSLGVVLYEAVAGALPFDGDNPITIA
                     LKHIHESPPFPSRNDAPVSRETERIILKALAKDPRDRYQSAEEMREDLIGVAAHWGET
                     PLPGVGDTAVVRPDGRTARRPRWPAPPALAGPLLAVAAGTAVVVVAALLGWQALVTYL
                     NVPEVTVPDFVGKDLADAQSRARRDHLNVQVVQESYSRTVPTGFVLGQDEPPGKVVKV
                     DRVIGLTTSLGPEMVSVPDVRRRSLLDARFMIEQARLVLGEVREAYDASVPPGVILSQ
                     DPAPGASLGRGTAVYLRVSKGPETLILPDLVGRPLDAARRMLDDLGVTLRQVTQVPGG
                     GIPPGQIMEMSPPAGTRIRHGDAVTVTIAAAPGATGTPPPQPIVTGTPLAPLTPGDPT
                     RKQARITVVVGEGAPRQVVKVVVVDAQGARVAYEKAHAPGDTVSTQVTGSGYTIVQVY
                     IDNRLIQEFRP"
     gene            8147..>8413
                     /locus_tag="VEW91_11385"
     CDS             8147..>8413
                     /locus_tag="VEW91_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYM92224.1"
                     /translation="MAGQAPSVQIAPSILAADFGRLGEEVRAAEQAGAARIHIDVMDG
                     RFVPEITLGPVVIQAVRRATSLPLDVHLMIVEPDRHLGAFARAGA"
BASE COUNT         1062 a         3021 c         3133 g         1197 t
ORIGIN      
        1 ccgggggcgg cgcgggacgt tccgctatca gtgcgtgttg cgggggtccg acggccgggc
       61 ggtccgggcc gctgcccgcg cggcgctcga gggagcgccc acgaaggcgg ggagccgggt
      121 cacggtggag atggacccac aggagtttcg ctagcgccca tggagatcat taccgtagat
      181 agtcccaagg cgagcatcct gcgccgccgc gcgcaaccgg tccggacggt cacccgcgaa
      241 atccgcgggc tgatcgatca gatgtttcac gcgttgcagg gggcccacgg gctgggcctg
      301 gccgccccgc aggtcggggt gagccggcgg ctcttcgtcg cccgactgga cgaccggcag
      361 atcgccctcg ccgatccgga gctgcttcat caggaggggg aacaggtcgc caccgaggcg
      421 tgcctgagca tccccggcct cctgggggat gtggcgcggg cgacgcgggt cacggtgcgg
      481 gggcgcaacc gccacaaccg gtttgtgacg atcgaagcca ccggactgct cgcgagagtc
      541 ctcctgcacg agatcgatca cctgaacggg atcctgttca ccgaccgggt gcaggacccg
      601 aagaccctgc ggcgggtgga gggcacctcc gaggtcgccc cggcaacggt cgcgtgatga
      661 agctggtctt catggggacc ccggagttcg cggtgccgtc gctcgaggcg ctcctcgccg
      721 ccagcgacct cgccgcggtg gtgacgcggc cggaccggcc ccagggtcgg gggcttcggg
      781 tcaccccgcc gccggtcgcc gtggccgcgg cccagtatgc gctcgacgtg ctccagcccg
      841 gcacgctccg ggaccccgcg ttcctggctc gcctgcgaac ccttgcaccg gatctcctcg
      901 tggtcgtggc gttcgggcgg ctcattcccc ccgaggtgct ggcgctggcc cccctgggcg
      961 gcatcaacct gcatccctcg atgctgccgc ggtaccgggg cgccgcgccg atcccccggg
     1021 cgatcgcggc gggggagacg acgaccggcg tcaccgtgct ccacctgagc gaggaactgg
     1081 acgccggcga catcatcctg cagcgtgcgg tgccgatcca tcccgacgac acgaccgcca
     1141 ccctggaagg ccgcctggct caggaggggg cggggctgct cgtggaggcg gttcgtctcc
     1201 tggagaccca tcaggccccg cgaacgcccc aggatccctc gcgcgtcacg ttcgcgccca
     1261 agatcgcccc ggaagaggcg tgggtctcct ggggcgatcc cgcggtcaag atcgtgaacc
     1321 aggtgcgcgc cttcgatccc tggcccgtgg cgtgcacacg gcgggacggc gtcctgctca
     1381 agatctggcg ggccgcggcg ttgccgggga gcgggcccga ggcgccgggg accgtggtca
     1441 gcgccgggcg cgaggggctc gtggtcgcct ccggcgcggg gagggtccag atcctggagg
     1501 tgcagcctgc ggcgggccgg cgcatgcccg cggccgacta cctccgcggg cacccgctcg
     1561 cacccgggac catgctcggc cctccgcggg gatgactgtg ctagaattaa gtgtcgggag
     1621 gtgacacgga tgttctggcc gctgtgggat ccgacgatga tcattgtcat cccggcgctc
     1681 ctcttcgcgc tctacgcgca gttccgggtg aagtccacct tcgcgcggtg gagcgaggtg
     1741 cggatcgcca atggccgcac cggcgcggag atcgcccggg agatcctccg ccgcaacggc
     1801 ctctccgagg tcgagatcga ccggaccgac gggatgctct ccgaccacta cgatccccgc
     1861 acccgcacgc tgagcctgtc ctcggacgtc cacgacggga tctccgtgtc gtccgccgga
     1921 gtcgccgcgc acgagaccgg ccacgcgatc cagcacgcgc gcgactacgc cccgctcgcg
     1981 ctgcgctccg ccatggtgcc gaccgtccag ttcgggtcct ggctggcctg gcccctgttc
     2041 ttcgtgggcc tgattttcca ctccggggcc atgatccaga tcggcatcct gatcttcacg
     2101 gtggccgtgg cgttcacgat cgtgacgctg ccggtcgagt tcgacgccag cgcccgcgcg
     2161 atgcgcgcgc tccgagaaga ggggctggtc accgccgacg aactgggcgg cgtgcgcgcc
     2221 gtgctcactg cggccgcgct gacctacgtc gccgccgccg cgatggcggt cctccaactg
     2281 gtccgcatgc tgctgctcgc taggcgggag gagtgatctt cccgcgccgc atgcaacgct
     2341 ccccatggct ggcccgccgg gcatcccccg gacctcgcgg gaggtggctc tagaggtcct
     2401 gcaccgcgtc gacacccacc aggcctggag cacggtgctc ctccggcgca tgctcgaccg
     2461 cgccgcgctc cgcccggcgg acgcagcgct ggccacggag ctgacctacg ggacccttcg
     2521 ccaccgcgcc gaactcgact gggtgctcgc gcgcgtcacg cgcaccccgc ttgacgagct
     2581 cccttcgcac attcgcgccg tcctcaggat gggcgcctac cagctgatgt ttctggatcg
     2641 gatcccgccc cgggcggcct gctccgagag cgtcgacctg gccaagcggg tcggccatcc
     2701 gggaaccgcc cggctggtca acgcggtgct ccgccggatc gcggcggcgc cgatcgcgat
     2761 tcccgaggac gagtcgacgc ccgacgggat cgcgctccgc cgctcgcacc cgctctggct
     2821 cgtgcgccgg tgggtggacc gcttcggggt ggcgggagcc cgcgcgctgt gcgcggcgaa
     2881 caacaccacg ccaccggcgt ccgtccgcct gaacaccctg cgcgggtccc cggcggcggt
     2941 ggcgaacgcg atcgcagcgc tcggggtgca caccatgcgc tcggcgctcc tccccgaagg
     3001 cgcccgcatc atcgaggcct cggcggaggc ccggcaggcc gcgtatgcgg ccgggtggat
     3061 ctccccgcag gatgagggat cgatgctggt gagccggctt gtggctccgc tccccggcga
     3121 gacggtcctc gacgcgtgtg cggggtcggg gggcaaatcg ctgcacctgg gcgcgctgat
     3181 ggggaaccag ggccggatcc tcgcgtgcga cgtcgtgccg gccaagctgg acgccatcgt
     3241 ccggcaggcg cggcggttgg gcgtcgcgat cgtcaccccc cgcctcctgg atgcgacccg
     3301 cctggccgcg gccctctcgt cggacgccga tcgcgtcctg gtcgatgcgc cgtgttcggg
     3361 cctcggcgtg atccgccgcc ggccggagat ccgctggcgg ctccagcccg agcagttgcc
     3421 cgcgctcgcc gcccgccagc gtgagatcct agacggggcc gcagccgcgg tgcgccccgg
     3481 gggcctgctg gtcttctcgg tgtgcacgat cgagccggac gagggggaga cggtggcatc
     3541 cggcttcctc gccgcccacc ccgagttcag ccccgagccg atctccggat ggccgcccgg
     3601 gagcggcgag ggtcccgctc cgtccccggt gcccggccgg ccggggaccg cgtacctcta
     3661 tccgcacgtg cacgacaccg acgggttctt cgtggcggcg ttccgccgga gccgatgatg
     3721 gcggccccat cgcctgccct cctcgaactg cgcgggctcc tgcgcgccga tctcgaggcg
     3781 ctcgcggccc gcctggggga accccggtac cgcggccggc aggtggcccg atggctgtac
     3841 gttcgcgggg cggagagcgt ggaggcgatg accgatctcc cccaggcgtt tcgggaccgc
     3901 ctcgcggagg gcgcccggat cgggcggctg accgtgcgcc aaacgcagga tgcgcccgac
     3961 gggtcggcga ccaagtacct ggtggcgctc gaggacggcc agatgatcga gtgcgtccgg
     4021 atgcggttcg acgacggccg gcgcagcgcg tgcatcagca cccaggccgg gtgcgcgatg
     4081 gggtgcgcgt tctgcgccac cggcctgggc gggtttgccc gcaacctctc cgccgccgag
     4141 atcctggggc agttcctgct catccgggcg catgggcggc ggcggctcac gaacgtcgtg
     4201 ttcatgggga tgggcgagcc gctggccaac tacgacgcga cggtcagggc ggtccggatc
     4261 ctcgccgccc cctacggcat gggcatcggg atgcgccaca tcacggtctc gaccgtcggg
     4321 ctcgtcccgc agatccgccg gctcgcgaaa gaacgtttgc agatgacgct cgcggtctcc
     4381 ctgcacgccc ccaccgacgc cctccgcgat cggttggtgc cggtcaaccg gcgctatccg
     4441 ctcgcggacc tgatggccgc gtgccgggag tacgtggagg cgaccggccg gcggctgacg
     4501 ttcgagtacg tgctgatcga tggcgtcaac gacgggccgc aggaggcccg cgcgctggtc
     4561 cggctcctcg ggggggtccg gtgccacgtc aacctgatcc cgctcaaccc cgtcgcgggc
     4621 atcccgttcg tgcggcctcc gattccccga gtccgggcgt tcgcggccgc cctgcggacc
     4681 gccggcatgg ccgtgacggt gcgcatcgag cggggcgggg agatccaggc cgcgtgcggg
     4741 cagctccggc tggccgacgg cctcggccgt ccctcgcgct ggacgccggt tgcggccgcg
     4801 gcgtcctccc ccgccgggcg gggtgggggg cgatgagcgg tagggcccgc agcgataccc
     4861 gcgtgtaccg cccgccggcg ccggggcccc ggctgcgcgt cctggccggg cctccgggcg
     4921 aggccggccg ggagttcgcg ctcgacggcc cgatggtgac catcgggcgg cggcccgacc
     4981 agcgcatcgt gctgagcgat ccgagcgtca gccgatccca cgcgcgcgtc gaggtggggg
     5041 cctggggcgt cgcgatcgtc gatctcggca gcacgaacgg caccgaggtc aacggccatc
     5101 gtctccgcga cgggcgcgcg gtgctccgcg gcggcgaccg catccggctc ggcaccgtgc
     5161 tcctcgagtt tctggcggcg cgatgaccgc gggcaagcgc ctcctgcctt tggcctcccg
     5221 cgcggccggg aagagcgata tcgggcgggt ccgggaggtg aacgaggacc gcatcctcct
     5281 gctcgatccc gcgggacggg gcatggccgt ctacgcggtc gccgacgggc tcggcggaca
     5341 tgcgggcgga gacgtggcga gcacgctggc cgtggagacg ctccgcaccg aactgccggc
     5401 gctgctggcg cagcgggtgc cggcgcggga agcgctcccc gaggccctgc ggcgggcgaa
     5461 cgcggggatc tatgccgcct cccaggggac ggaccgggcg ggcatggcga cgacctgcac
     5521 cgcggtgctg gtcgatgggg gggagggggt ggtggcccac gtcggtgaca gccgcgcgta
     5581 tctgctgcgc ggccaggcgg tgcggcagct gaccaccgac cattcgctgg ccggggaact
     5641 cgcgcgccgc ggcgccccgg tcgaggcggc ccacgcgcaa cgccatgtgc tcacgcgcgc
     5701 gctgggcacc gcccgggatg tcgagatcga ttgcgtcgcc gagcccctga gggccggcga
     5761 tgtgctcgtg ctcacgaccg acggcctgca caacgccgtg cccccggagg agatcggcgc
     5821 ggtcgtgcgg gccgccgcgg atctggagga tgcgtgccgg acgctggtgg gcctcgcgaa
     5881 cgcgcggggc ggcgtggaca acgcctcggc gatcgtcatc cgcctgaccc cgtggcgggg
     5941 gggacgcgtg atgcgcctgc tcgccccgat cgggctggcc gcgttcctcg ccgggggcgt
     6001 ggcggtgtac cggctggaac acgcctactt tctcggcgtg cgcgacgacc gggtcgcggt
     6061 gatgcggggg gtccccggcc gcgtgctcgg cgtgccgctc tccggggtgg tcagggtgac
     6121 ccaggtcccg gtgacccgga tcgcgcccgc cgatcgcagc cggctcgccc gcggcatccc
     6181 gacggccagc ccggaggagg cggagagcat gctggacggc ctgctcttcc atccgtagca
     6241 tgatcggccg gtccctcggc gggaagtacg aggtcacggg gccgctcggc gaggggggca
     6301 tggccacggt gtaccgcggc cgccggctct cggacggccg cagcgtcgcg atcaagatcc
     6361 tgcgtgagca gtatgcgcac gacacggagt tcgtcgcccg gttcgaacgc gaagcccagg
     6421 cggtcgcccg gctctcccat ccccacatgg tccaggtgtt cgattccggt cgggatgggg
     6481 atgtgcacta catcgtgatg gagtttgccg aaggggaaga tctcaagacg ctgctccgcc
     6541 gggtccgggt gttatccgag cagcgggccc gggagatcgg cgcccaggtc tgcgaggttc
     6601 tggcgtacgc ccatgcggag ggcatcgtcc accgggacat caagccgcag aacatcctgc
     6661 tgaccttcga tgggcaggtg aaggtgaccg atttcggcat cgcccgggcc ctggcatcgg
     6721 ccgcgatcac ggagaccggg acggtcttgg ggtcggtcca gtacctgtcc cccgagcagg
     6781 cccgcggcct cggcgtggga cagagcaccg atctctactc gctcggtgtg gtgctctatg
     6841 aggcggtggc cggggcgctg ccgttcgacg gggacaaccc gatcacgatc gcgctgaagc
     6901 acatccacga gtctccgccg tttccgtccc ggaacgacgc gccggtgagc cgggagaccg
     6961 agcggatcat cctgaaagcg ctcgccaagg atcctcggga ccgctatcag tccgcggagg
     7021 agatgcggga ggacctcatc ggggtggcgg cccactgggg ggagacgccg ctccccggcg
     7081 tcggggatac cgcggtcgtc cggcccgacg gccgcacggc gcggcgaccc cggtggccgg
     7141 cgccccccgc gctggccggt ccgctccttg cggtcgccgc ggggacggcc gtcgtcgtgg
     7201 tcgcggcctt gctcgggtgg caggcgctcg tgacctacct gaatgtgccc gaggtgaccg
     7261 tgccggactt cgtcggcaag gacctcgcgg acgcgcagag ccgcgcccgg cgggaccacc
     7321 tgaacgtcca ggtggtccag gagagctaca gccggaccgt gcccacgggg ttcgtgctgg
     7381 ggcaggacga gccgcccggc aaggtggtga aggtcgaccg cgtcatcggg ctgaccacca
     7441 gcctcgggcc cgagatggtc agcgtgccgg acgtccgtcg gcggtcgctg ctggacgcgc
     7501 gcttcatgat cgagcaggcc cggctggtcc tgggcgaggt gcgggaggcg tacgatgcca
     7561 gcgtgccccc cggcgtcatc ctcagccagg atcccgcccc gggggcctcg ctggggcgcg
     7621 gcaccgcggt gtaccttcgg gtgagcaagg ggccggagac gctgatcctc cccgacctgg
     7681 tggggcgccc gctcgatgcg gcccgccgga tgctcgacga tctcggggtg acgctccggc
     7741 aggtcacgca ggttcccggc ggcgggatcc cgcccgggca gatcatggag atgtcgccgc
     7801 cggccggcac ccggatccgg cacggggacg ccgtgactgt gacgatcgcc gcggcccccg
     7861 gggccacggg cacgccgccg ccccagccga tcgtgacggg cacgccgctc gcgcccctga
     7921 ccccgggcga tcccaccagg aagcaggccc ggatcaccgt cgtggtgggt gagggcgccc
     7981 cgcgccaggt cgtcaaggtc gtcgtcgtcg atgcgcaggg cgcgcgcgtc gcgtacgaga
     8041 aggcccatgc gccgggcgac accgtcagca cccaggtcac cgggtcgggg tacacgatcg
     8101 tgcaggtcta catcgacaac cgtctgatcc aggagttccg gccctgatcg cgggtcaggc
     8161 gccgagcgtc cagatcgccc cgtccatcct cgccgccgac tttggccgtc tcggcgagga
     8221 ggtccgcgcc gccgagcagg cgggagcggc ccggatccac atcgatgtca tggatggccg
     8281 gttcgtcccg gagatcacgc taggcccggt ggtgatccag gccgtccggc gggccacgtc
     8341 cctgccgctc gacgtgcacc tgatgatcgt ggagcccgat cgccacctcg gggcgttcgc
     8401 aagggcgggg gcc
//
LOCUS       DASQGH010000575         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_25330, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000575 DASQGH010000000
VERSION     DASQGH010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25330"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VEW91_11390"
     CDS             <1..426
                     /locus_tag="VEW91_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYM92225.1"
                     /translation="ENMLVPGLTRPGSTRAMVERRARTYLEFLNLAHLQDLSAKNLSG
                     GQQKLLELGRALMLEPAILFLDEPFAGVHPHLRDELIQRIQQLHQSGRTFVVVDHDME
                     SILRVARRLVVMARGQKIADGPPAVVRSDQTVLAAYSGL"
     gene            479..1183
                     /locus_tag="VEW91_11395"
     CDS             479..1183
                     /locus_tag="VEW91_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92226.1"
                     /translation="MAGYLPGVSILQGVSVDAIRGQIRCVLGPNGTGKSTLLKVLFGF
                     LPPWEGEVFLDGRPINGVAPHEMGRRRVAYLPQRPSVFPYLSVEVNLRLGAWQYKAER
                     GRVEALVQRACDQFPILREKRRHPAGTLSGGQQRQLEIARSLMHDPVVFLIDEPTAGI
                     EPRVAAQIYGIIKEIAGQDKAILLVDQNIKRALEIADYVYVMRTGTILSEGSRESFGG
                     DTETLVARWLYESGKA"
     gene            1187..1957
                     /locus_tag="VEW91_11400"
     CDS             1187..1957
                     /locus_tag="VEW91_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HYM92227.1"
                     /translation="MAGPAVLVQACLNGSRRSDEHEALPVSPQGLAAAARGAVAAGAA
                     ALHVHPRLPGGAETLEPAACGETVREILAACPGVPVGLTTGAWIDPDPRHRLLQLSRW
                     TVRPDFVSVNFSEPGALAICEWLIHHGIGIEAGLATIADAGVLVESGVAHRCLRVLVE
                     VEDPDPGAAVEDAQAIDALLDAHGVTVGRLHHGVGLATWAVLDAALDRGRDIRVGFED
                     TLHLPDGRRAQDNAELVAAAVDLACRHGRSIVSPGHHA"
     gene            complement(1962..>2554)
                     /locus_tag="VEW91_11405"
     CDS             complement(1962..>2554)
                     /locus_tag="VEW91_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYM92228.1"
                     /translation="SLVGLHTGHLRIGASRTAGAYYIAGLLDRFKHRYPGIKVSLSVG
                     NSETILARVLDFSLHAGLVAGPAEDPHLVSRPLIRDTMLVVLSPGHPLCGRTVVGIRE
                     LHRYPVILREPGSATRRIIERAFQGAGLEVSAAMELESNQAIKSAVAEELASGRLVGR
                     PLQEALRLDFALVYHRDRTPSPVVAAFLELLPPASR"
BASE COUNT          399 a          843 c          911 g          401 t
ORIGIN      
        1 gagaacatgc tggtgcccgg gttgacgcgg cccggatcca cccgggccat ggttgaaagg
       61 cgggcgcgca cctacctgga gttcttgaac ctggcgcacc tccaagacct gagcgccaag
      121 aacctctcgg ggggccagca gaagctcctc gagctcgggc gcgccttgat gttggaaccg
      181 gccattctgt tcctcgacga gcccttcgcg ggcgttcatc cccatctgcg ggacgaactg
      241 atccagcgca ttcagcaact ccatcagtcg gggcggacct tcgtcgtcgt cgaccacgac
      301 atggaatcca tcttgcgcgt cgcgcggcgt ctggtcgtga tggcccgggg gcagaagatc
      361 gccgacggtc caccggccgt ggtgcggagc gaccagaccg tgctcgccgc gtactccggt
      421 ctctaggatg atgctcccga cggacgggac ggcaccctcc ctagccgcga gaaagctcgt
      481 ggccgggtac cttcccggtg tcagcatcct gcagggggtc tcggtggacg cgatccgggg
      541 ccagatccgg tgcgtgctcg ggccgaacgg caccgggaag tcgaccctct tgaaggtgct
      601 gttcgggttc ctgccgccgt gggaaggcga ggtgttcctc gacggccggc cgatcaacgg
      661 tgtcgcgccg cacgagatgg ggcggcgcag ggtcgcctat ctcccgcagc ggccaagcgt
      721 cttcccctac ctgtcggtgg aggtgaatct gcgcctgggc gcctggcagt acaaagccga
      781 gcgcggccgc gtcgaagcgc tcgtgcagcg ggcgtgcgat cagtttccca ttctgcgcga
      841 aaagcggcgg catcccgcgg ggacgctgag cggcggccag cagcggcaac tcgagatcgc
      901 gcgcagcctg atgcacgacc ccgtggtctt cctgatcgac gagcccaccg cggggatcga
      961 gccgcgggtc gccgcacaga tctacggcat catcaaggag atcgcggggc aggacaaggc
     1021 gattctactt gtggaccaga acatcaagcg ggcgctggag atcgcggact acgtgtacgt
     1081 gatgcggacc ggcacgatcc tttcggaggg gtcgcgggag tcgtttggcg gggataccga
     1141 gacgctggtc gcccggtggc tctacgagag tgggaaagcg taggcggtgg cgggccccgc
     1201 agtccttgtg caggcctgcc tcaacggttc acggcggtcc gacgagcacg aggcgctccc
     1261 ggtgtctccc caggggctcg ccgctgccgc ccggggcgcc gtggcggcgg gagccgcggc
     1321 gctccacgtg catccgcggc ttcccggtgg ggctgagacg ctcgagcccg ccgcgtgcgg
     1381 ggagacggtc agggagatcc tggcggcatg ccccggcgtg cccgtcggcc tgaccacggg
     1441 cgcatggatc gaccccgatc cgcggcaccg gctcctccaa ctctcccgct ggacggtccg
     1501 gcctgatttc gtctccgtca atttctccga gccgggggcg cttgcgatct gtgagtggtt
     1561 gatccaccac gggatcggca tcgaggccgg tctcgcgacc atcgccgacg ccggcgtgct
     1621 cgtcgagagc ggggtggctc accgctgcct gcgcgtgctc gtcgaggtag aggaccccga
     1681 ccccggggcg gccgtggagg acgctcaggc cattgacgcc ctgcttgacg cgcacggcgt
     1741 cacagttggg agactgcacc acggcgtcgg cctggcgacc tgggcggtcc tcgatgcagc
     1801 gctcgatcgc ggacgcgaca tccgggtggg attcgaggac acgttgcacc tccccgatgg
     1861 ccgccgggcg caggacaatg cggagctcgt ggctgcggcg gtcgatctgg cgtgccgcca
     1921 tggccggtcg attgtgtccc cgggccacca cgcatgatgc ctcaacggga cgccggagga
     1981 agcagctcca ggaacgcggc gacgacgggg gagggcgttc ggtcacggtg gtagaccaga
     2041 gcgaagtcca ggcgcagcgc ttcttggagg ggtcgaccca cgaggcgccc cgaggcaagc
     2101 tcctcagcca ccgcgctctt gatcgcctgg ttgctctcga gttccatggc ggcggagacc
     2161 tccaggccgg ccccctgaaa agcccgttcg atgatgcggc gcgtggccga gccgggctct
     2221 cgaagaatca ccggatacct gtgcagctct cggatcccga caaccgttcg cccgcagagc
     2281 gggtggccag gggacaggac caccagcata gtgtcgcgga tcagcgggcg ggataccaaa
     2341 tgtggatcct cggcggggcc agcgacaagc ccggcgtgaa ggctaaagtc gagcacccgc
     2401 gccaggatcg tctccgagtt ccctaccgaa aggctcacct tgatccccgg atagcggtgc
     2461 ttgaaccggt cgagcaagcc ggcgatgtag tacgcgcccg cggtccggct cgcgccgatc
     2521 cgcaggtggc cggtgtgcaa tccgacgagg ctgt
//
LOCUS       DASQGH010000576         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_13991, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000576 DASQGH010000000
VERSION     DASQGH010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13991"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VEW91_11410"
     CDS             complement(<1..104)
                     /locus_tag="VEW91_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL9 family ribosomal protein"
                     /protein_id="HYM92229.1"
                     /translation="MKVILLKDVPSLGRAGDIREVKEGYAQHYLLPRGL"
     gene            complement(135..1196)
                     /locus_tag="VEW91_11415"
     CDS             complement(135..1196)
                     /locus_tag="VEW91_11415"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HYM92230.1"
                     /translation="MKDRRPPAADPDRRRARPPTRGLTEGAVLAALAAVVAAAGLVIP
                     PIGIVLAPLPVMLLVIRWGMRTAVLASVVSGLILLQFFGPLTALSVTGMFVPLGLALG
                     WGTRRRAGPQITILAGSAAFLLSTLATLALTVFVLHQDVVGQLIRSQVQGMEMALGLQ
                     QRLGAPKAQIEEMHRAIAALPQFLYTAFPVMVALGALLWAYLCYLLARSVLRRVGHEI
                     AAVSPILSWRLPLSLTTGLLWVSMGLFLTSMRVPRLAGAALDAALVNLFVFGFQGALV
                     GITWMHARRVPRVAQIIAGGLVITSGLLPLAGLAIIGILDTWYDYRHLTRGGPVAERD
                     GVATKIAPPPRGGGAAPSG"
     gene            complement(1196..1456)
                     /gene="rpsR"
                     /locus_tag="VEW91_11420"
     CDS             complement(1196..1456)
                     /gene="rpsR"
                     /locus_tag="VEW91_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HYM92231.1"
                     /translation="MPEVREKDARPRRDYRRRPKRKVCSFCTDKATLIDYKDVNRLRR
                     FVTERGKILPRRVSGNCARHQRTLAVAIKRARELALLPYTGE"
     gene            complement(1496..1924)
                     /gene="ssb"
                     /locus_tag="VEW91_11425"
     CDS             complement(1496..1924)
                     /gene="ssb"
                     /locus_tag="VEW91_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYM92232.1"
                     /translation="MYNRIILIGRLTRDPELRYVPSGAPVASFTLAVDRPFKDQQGNR
                     ETDFIDIVAWRKLAEQVSQYMSKGRMVAVEGRLQIRSYETQDGQKRKVAEVVADGIRF
                     LDRGRASASEAAPAAPAIATAGAAAEEAAPGGSNDEDVPF"
     gene            complement(1917..2339)
                     /gene="rpsF"
                     /locus_tag="VEW91_11430"
     CDS             complement(1917..2339)
                     /gene="rpsF"
                     /locus_tag="VEW91_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYM92233.1"
                     /translation="MGAYEIVAVLRPDLDEEGLGAALDRISQRIVEHGGGITTLERWG
                     KRKLAYPIKKHRDGYYALIVFSLAGDRIGPLRQTLGLHEEMLRFAFSTHQPKPTPAAA
                     PAAPAAPGQTGTPSKPVTGSTSGTTGGRPAGAGETPHV"
     gene            complement(2517..3611)
                     /gene="ychF"
                     /locus_tag="VEW91_11435"
     CDS             complement(2517..3611)
                     /gene="ychF"
                     /locus_tag="VEW91_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HYM92234.1"
                     /translation="MSLTCGLVGLPNAGKSTLFRAITAAPAEIAPYPFTTIAPNVGMA
                     RVPDPRLDGIARITHPKRVVPATLEVIDLAGLVRNAHKGEGLGNQFLARIREVDALIH
                     VVRCFHGDVPHVEGTVDPLRDIGIVDTELLLADLGAIERARTHTAPRARAGERGARDQ
                     DQALAHLEAHLGAGNPARTCALPSGGPASAAPGYGQLLTARPVLYVANLSEEDLAGGP
                     CLEAVAGHAAREGAAAVGICAQLEMELAELPDAEAAEFLASLGVAERALPRLIRACES
                     LLNLRTFFSIASDEVRAWPVPAGTRASQAAGRIHSDMERGFIRAELVAYADLVACGSI
                     ASARERGAIRLEGRDYEVQDGDVITFRFAT"
     gene            complement(3680..>4569)
                     /locus_tag="VEW91_11440"
     CDS             complement(3680..>4569)
                     /locus_tag="VEW91_11440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYM92235.1"
                     /translation="ELHTTLNYPLVIIRRVDGDQLRHVAHRGYAGIQETSPLTAGIVG
                     RVARTGEAAFVTDVSGDPDYIAWDPRVTQEVCVPIVHQGRVVGVVNVEVIDPTLTRED
                     LNLLTTLAGYAAVALEKAQLYEQTQALAITDGLTGLLNYRAFWQALERELERSMRYGL
                     PLSLIMIEIDKFKRFNDSYGHLRGDEVIRAVARVLQQEHRAQIDIVARYGGDEFMILL
                     PHTQKVTAAEIAERVRRAVEATPLISAVEVASVTLSLGVACYPEDGKSADTLVEAVDR
                     SMYKAKQQGGNAVAVAKTS"
BASE COUNT          700 a         1555 c         1647 g          667 t
ORIGIN      
        1 agaccgcggg ggaggagata gtgctgggcg tacccttcct tgacctcgcg gatatcgccc
       61 gcccggccaa gcgagggcac gtccttcagg aggatgacct tcatgggtgg atacgcaccg
      121 atcgcgagct cgcgttagcc cgagggcgcg gccccgccgc cccggggagg cggggcgatc
      181 ttcgtggcca cgccgtcgcg ctcggccacc ggtcctcccc tcgtgaggtg gcggtaatcg
      241 taccaggtgt ccaggatgcc gatgatcgcc agaccggcca ggggcagcaa ccccgaggtg
      301 atcaccagcc cgccggcgat gatctgggca acccgtggaa cgcgccgggc gtgcatccag
      361 gtgatgccca cgagcgcgcc ttgaaaaccg aagacgaaga ggttgacgag cgcggcatcc
      421 agggccgcgc cggccagccg gggcacccgc atgctggtga ggaacaggcc catcgacacc
      481 cacagcagcc ccgtggtcag ggatagcgga aggcgccagg agaggatggg ggagaccgcc
      541 gcgatctcgt ggccaacgcg ccgaagcacc gaccgggcca gcaggtagca gaggtatgcc
      601 cagaggagcg cgcccagcgc caccatcacc ggaaaggccg tgtacaggaa ctgcggcaag
      661 gcggcgatgg cgcggtgcat ctcctcgatc tgcgctttgg gcgcccccag acgttgctgc
      721 aacccgaggg ccatctccat gccctggacc tggctccgga tcagttgtcc cacaacgtcc
      781 tggtggagta cgaagacggt gagcgcgagg gtggcaaggg tggagaggag aaacgcggcc
      841 gacccggcga ggatggtgat ctgaggcccg gcccgccgcc gggtccccca gccgagcgcc
      901 agcccaaggg gcacgaacat gcccgtcacg gacagcgcgg tcagcgggcc aaagaattgc
      961 agcaggatca ggccggagac gacggaagcg agcaccgccg tgcgcatccc ccaccggatg
     1021 acgaggagca tgaccggaag cggggccagg acgatgccga tgggcgggat aacgagcccc
     1081 gccgccgcga ccacagcggc aagggcggcg aggaccgcac cttcggtgag cccgcgcgtt
     1141 gggggtcggg cgcgccgacg gtccgggtca gccgcaggcg gccgccggtc cttcatcact
     1201 caccggtgta gggcaggagc gccagttcgc gtgcgcgctt gatcgctacc gccagggtcc
     1261 gctggtgccg ggcgcagttg ccggagacac ggcgcggcag gatcttgccc cgctcggtga
     1321 caaaccggcg aagccggttc acgtccttgt agtcgatcag cgtcgccttg tccgtgcaaa
     1381 acgaacagac cttgcgcttg gggcgacgcc ggtaatcccg ccgcggccgc gcgtcctttt
     1441 ccctcacctc tggcatctca gtcctcccga cagccgcccg gggcggcggt gcagatcaga
     1501 acggcacgtc ctcgtcgttg ctcccaccgg gggccgcttc ttccgcggcc gcgcctgcgg
     1561 tcgcgatcgc gggggcggcc ggcgccgctt cggaggcgct ggccctgcct cgatccagga
     1621 acctgatccc gtcggcgacg acctcggcga ccttccgctt ctggccatcc tgcgtttcgt
     1681 aggagcggat ctgcaggcgc ccctcgacgg ccaccatgcg ccctttggac atgtactggc
     1741 tgacctgctc ggccagcttc cgccaggcga cgatgtcgat gaaatcggtt tcacggttcc
     1801 cctgctggtc cttgaacggc cggtcgacgg ccagcgtgaa gctggccacc ggagcgccgc
     1861 tcgggacgta ccggagctcg gggtcccgcg tgagccggcc gatcaggatg atccggttat
     1921 acatgcgggg tctcccccgc tccggccgga cggcctccgg tcgtgcccga ggtcgacccg
     1981 gtcaccggct tggagggtgt ccccgtctgc ccgggggccg cgggggcggc tggggccgct
     2041 gctggggtgg gtttgggctg gtgcgttgag aaggcgaacc ggagcatctc ttcgtgcaat
     2101 ccgagcgtct gccggagcgg cccgatgcgg tcgcccgcga gcgagaagac gatgagggcg
     2161 tagtagccgt cgcggtgctt cttgatgggg taggccagct tgcgcttgcc ccagcgttcc
     2221 agggttgtga tcccgccgcc gtgctcgacg atccgctggc tgatgcgatc gagcgccgca
     2281 cccagcccct cttcgtccaa gtcgggccgc aacacggcaa caatctcgta cgcgcccatt
     2341 gggcacgctc ccctcctctg gactgaaacc gggcatcccg gcggccctgt gtgcccctgc
     2401 cccgcgggta cctcggcggg gctccgggca gggcaggaag gatccggcca cgcccgcgcg
     2461 cggccgcaga gattgtacca cggcccggtt gcagcgcaca agccgtcgcc ggagggttac
     2521 gtggcaaacc ggaaggtgat cacgtccccg tcctggacct cgtaatcccg tccctccagg
     2581 cggatcgccc cgcgctcacg agcggaggcg atcgagccgc acgccacgag atccgcatac
     2641 gccaccaact cggcacggat aaacccccgc tccatgtcgc tgtggatgcg cccggcggcc
     2701 tgggaggccc gggtgccggc cggaaccggc cacgcccgga cctcgtcgga cgcaattgaa
     2761 aagaaggtcc gaagattcag cagcgactcg catgcgcgga tgaggcgggg cagggctcgc
     2821 tcggccacgc cgagggacgc cagaaattcc gcagcctccg cgtccggcaa ctcggccagc
     2881 tccatctcga gctgtgcgca aatccccacg gccgcggccc cctcccgcgc ggcgtgcccg
     2941 gcaaccgcct ccaggcacgg gccgcccgcc aggtcctcct ccgacaggtt ggcgacgtag
     3001 aggaccggac gcgcggtcag gagctgcccg tagccgggtg cggcggaggc cggcccgccg
     3061 gacggcagcg cgcacgtccg cgccggattg ccggctccga ggtgggcttc caggtgcgcg
     3121 agggcttggt cctggtcccg cgccccccgc tcgcccgccc gggcgcgcgg ggcggtgtgc
     3181 gtgcgggccc gctcgatcgc gcccagatcg gccaggagga gctcggtgtc cacgatgccg
     3241 atgtcacgca ggggatccac cgtgccctcc acatgcggga catccccgtg gaagcagcgg
     3301 acgacgtgga tgagggcgtc gacctcccgg atccgggcga gaaactgatt ccccaacccc
     3361 tcccccttat gggcgttccg gaccagcccg gcgagatcga tcacctcgag ggtggcgggg
     3421 acgacgcgct tgggatgggt gatccgcgcg attccatcca gccgggggtc ggggacccgg
     3481 gccatgccga cgttcggcgc gatcgtcgta aaggggtacg gggcgatctc cgccggggcg
     3541 gcggtgatcg cgcggaacag ggtggacttc ccggcgttgg gaagaccgac gaggccgcag
     3601 gtcagggaca cgcagcgcct ccggcgcggg ctgaggcgcg cctgcgtatg gcgtggggca
     3661 gcggcaggtc agcgctcgct caagaggtct tcgccacggc cacggcgttt ccgccctgct
     3721 gtttggcttt gtacatcgag cggtcgaccg cctcgacgag cgtatcggcg ctcttgccgt
     3781 cctcggggta acaggccacc ccgaggctga gggtgaccga ggcgacctcg acggcgctga
     3841 tgaggggtgt ggcctccacc gcccggcgga cgcgctcggc gatctccgcc gcggtgactt
     3901 tttgggtgtg ggggaggagg atcatgaact cgtcgccgcc gtagcgggcg acgatgtcga
     3961 tctgggcgcg gtgctcctgc tggaggaccc gggcgaccgc ccggatgacc tcgtcgcccc
     4021 gcaggtggcc gtaggagtcg ttgaagcgct tgaatttgtc gatctcgatc atgatgaggg
     4081 agagcgggag gccgtagcgc atggaccgtt caagctcgcg ctcgagcgcc tgccagaagg
     4141 cgcggtagtt gaggaggccg gtcaggccgt cggtgatggc gagggcctgg gtctgctcgt
     4201 agagctgcgc cttttcgagc gccacggcgg catatccggc aagcgtggtg aggaggttca
     4261 gatcctcccg ggtgagggtg gggtcgatga cctccacgtt gaccaccccc acgacccgcc
     4321 cctgatgcac gatggggacg cacacctcct gggtcaccct gggatcccac gcgatgtagt
     4381 ctgggtcacc ggagacgtcc gtgacgaacg ccgcctcgcc ggtccgggcg acccggccga
     4441 cgatgcccgc ggtgagcggg gatgtctcct ggatgcccgc gtacccgcgg tgcgccacgt
     4501 ggcgcagctg gtctccgtcg acgcgccgaa tgatcacgag cggataattc agggtggtat
     4561 ggagctctt
//
LOCUS       DASQGH010000577         8816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36509, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000577 DASQGH010000000
VERSION     DASQGH010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36509"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VEW91_11445"
     CDS             complement(<1..679)
                     /locus_tag="VEW91_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYM92236.1"
                     /translation="MSMNAPSTPPRRRGPGYLAGAIAAMAVVAVGALLGGIVLPQHLG
                     LHPVFSAQPVAQAPAPPGTAPAPSVGGTVRVVPEESVIISVVKQVRPAVVNINTESQV
                     QTVFGVFPQQGAGSGVIVRSDGYIVTNNHVVQDATTIKVTLIGGKTLTGRIIGRDPLA
                     DLAVIKVDAKDPLPAARLGGSGNLQVGQLAIAIGNPFGLGSTVTTGVISALNRNIELP
                     NLDVENLI"
     gene            867..1781
                     /gene="accD"
                     /locus_tag="VEW91_11450"
     CDS             867..1781
                     /gene="accD"
                     /locus_tag="VEW91_11450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HYM92237.1"
                     /translation="MLNWISRPKYASVERRDVPAGLWTKCPRCAALVYRKELERNLRV
                     CPRCTYHHRLSAADRLAMVLDSGSFVEHDEGLVPEDPLGFVDEKPYPARVDQAQRQTG
                     LPEAIVTGTGAIEGRRAVVGAMEFGFLGGSMGAVVGEKVARSAERAGSLRWPLVLFSA
                     SGGARMQEGALSLLQLAKTSVALGRLAEAGIPFLSVLCDPTTGGVAASFAFQGDVIIA
                     EPGALIGFAGRRVIEQTIRQKLPEGFQTAEFLLEHGLIDAIVPRGDLRPTLGRLLRLC
                     GAPLGPSPSDSPQATPTEGRSADDERSA"
     gene            1765..2814
                     /locus_tag="VEW91_11455"
     CDS             1765..2814
                     /locus_tag="VEW91_11455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HYM92238.1"
                     /translation="MSVLPDPAGAAGARDDAADERLERDLAEAEREIAELKRITATTQ
                     GMDMAHQIALLEARAEELREAIASNPTPWQTVQLARHAERPKIGDYIAAITTDFIELH
                     GDRTFRDDPAIVAGLGFVDGRTVAVIGHAKGRDTRENIARNFGMPNPEGYRKAVRIMH
                     LAEKLRAPVVTLIDTPGAFPGREAEERGQSEAIARALIGMGRLRTPVVTVITGEGGSG
                     GALAIGVGDVVLMLERAIYSVISPEGCAAILWRDASRGREAARALRLTAPDLVAFGIV
                     DEIIPEPPGGAHRHPDRAAAAVAAAVHRHLAALADRPVELLLEARYAKFRRIGRLRDA
                     GQVPGRSPSEHTASE"
     gene            2869..3324
                     /gene="accB"
                     /locus_tag="VEW91_11460"
     CDS             2869..3324
                     /gene="accB"
                     /locus_tag="VEW91_11460"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HYM92239.1"
                     /translation="MSQERRLDLEEIRALIRLATEADITELDVDAPHVKVRIKKAARN
                     PVEPLAAKVPRPGGAIPEAASAASEGGLVPITAPMVGTFYRAPKPDAPPFVSEGDEVR
                     VGQTVCILEAMKLFNEISSDVGGRIARVLAENGAPVEYGQPLFLVDPAV"
     gene            3338..4699
                     /gene="accC"
                     /locus_tag="VEW91_11465"
     CDS             3338..4699
                     /gene="accC"
                     /locus_tag="VEW91_11465"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HYM92240.1"
                     /translation="MATFSKVLIANRGEIAVRIIRACRELGLSTVAVYSEADRGALHT
                     RLADEAFCIGPAPARESYLNIPSIISTAELLGVDAIHPGYGFLAENPHFAEVCRDCGI
                     TFIGPSPEAIEKMGNKSAAREMVRKAGVAIVPGSDGPVRDEAAAAGVARSLGYPLIIK
                     AAGGGGGRGMRVVHNRDDLRRALTEARGEAEAAFGNTEVYVEKYLEEPRHVEVQVLAD
                     TRGTVITLGERDCSVQRRHQKLIEESPSPGVGPKLRRVLNRAAARVAEASGYTNAGTV
                     EFLVDQAERFYFVEMNTRIQVEHGVTEMVTGVDLVKEQLRIAAGERLAIPREVEPRGH
                     AIECRVNAEDPARDFLPSPGQIVAFVPPGGPGIRVDTHAFAGYTVPPHYDSLIAKVIA
                     WGQDRDEAITRMQRALGEFEITGVQTTIPFHQDALDNAFFRRGEVYVNFVQRRMDLTA
                     LRA"
     gene            4717..5622
                     /locus_tag="VEW91_11470"
     CDS             4717..5622
                     /locus_tag="VEW91_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYM92241.1"
                     /translation="MRGAITIPLPTPFSVPRTLSFLRTSSLRTPYHFVGAHRVRRLVR
                     LDGRPSVVEFGFAPPGARLRVRAVGPVEGPGLASALRRLATSVWSLDDDLRRCYAALR
                     RDPLMAPLLKHCAGLRMIRTPDLYEALLIAIIGQQVSVAAAESIRRRLITTLGDRAVR
                     EGMTYAGYPPPRRLLRTSPAGLRAVGLSRQKARYVLEIADREVAGVLAPAAFDGLPDE
                     QAIARLIEIPGVGRWTAEIVLMRGLGRPDVFPAADLGLGLAVQRLLGRRTRPTEDALR
                     ALADRWMGWRSYGALYLWRSLGIAA"
     gene            complement(5646..6647)
                     /locus_tag="VEW91_11475"
     CDS             complement(5646..6647)
                     /locus_tag="VEW91_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYM92242.1"
                     /translation="MTSAEMNAPALAVDLGGTKLLVGVVGRDGRVLARQRIAPPQGGP
                     EPVARAIRDLARSLPRGAAGTAGASLAGVGVAFAAPTDHDRGVVYDPPNLTGWGRETA
                     FGPLLARELGETVYVENDANAAALGEAWTGAGRGVRDLVYITVSTGIGGGLILGGRLY
                     RGANGTAGEIGHIIVEPDGPRCHCGNRGCLEVLASGTAIARQAREAVARGEPTALGRL
                     AAHPEEITAESVAEAARGGDALAAELYRRAGTRIGLVLSNLLALLTPKMIIVGGGVSK
                     TGDLLFRPLRDAMRARVYPAPALGVEVVPADLGDDVGIIGAAALVYHAAGGRSVGEA"
     gene            6849..7721
                     /locus_tag="VEW91_11480"
     CDS             6849..7721
                     /locus_tag="VEW91_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011752498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYM92243.1"
                     /translation="MVGYVLAVLIVAVLLLWNGIKIVREYQRLVVFRLGRSFGPRGPG
                     IVYLIPIVDKAVWVDLREVYLEIPAQTCITKDNAPISIDFLIYWKVVDPQLSVIQVGN
                     FAGAAQGIATTGLRAVIGDISLDDVLAKRDQINQVLRAKLDEVTERWGVKVTTVEIRE
                     ITPPKDVQEAMTRQMSAERSRRAIVTEADGKKQAAITIAEGEKQAAILKAEGDRQAAI
                     LRAEGFALALGKIFEVARTVDTNTMSLQYLEALKALGAGPATKFVFPMEFTKLLQPFT
                     DFAGRATPPPPKTP"
BASE COUNT         1411 a         3006 c         3084 g         1315 t
ORIGIN      
        1 ggatcaggtt ctcgacgtcg aggttgggga gttcgatgtt gcgattcagc gcgctgatca
       61 cgccggtggt caccgtgctc cccagcccga acgggttgcc gatcgcgatc gccagttggc
      121 cgacctgcag gttgccgctg ccgcccagcc gcgccgccgg cagcgggtcc ttggcgtcca
      181 ccttgatgac cgccaggtcg gccagcgggt cacggccgat gatccgaccg gtcagcgttt
      241 ttccgccaat gagcgtcacc ttgatcgtgg tcgcatcctg caccacgtga ttgttggtca
      301 caatgtaccc atcggaacgg acgatcacgc cggaccctgc gccctgctgg gggaagaccc
      361 caaacaccgt ctgcacctgc gactccgtgt tgatgttgac gacggccggc cggacctgtt
      421 tgacgacgct gatgatcacc gattcctcgg ggaccacgcg gaccgttcct cccaccgacg
      481 gcgcgggtgc cgtgcccggc ggcgccggcg cctgggccac cggctgcgcc gaaaagaccg
      541 ggtgcagccc caggtgctgg ggcaggacga taccgccgag cagggccccg accgccacga
      601 ccgccatggc ggcgatcgcg cccgcaagat agccggggcc gcggcggcgc ggcggtgtgc
      661 tcggtgcgtt catggacatc gagaatccct ccctgtcttt catggcatcc ctattatagc
      721 atccacgttt ttccacccaa tgtcgcccgg ctgagcggtt cggggcatcc actcaaacag
      781 tttgccgcct tcgggagtcg tacggtataa tcgaaccgat caaaggcacc tcaccggcgc
      841 ggcagcgcaa ctggagcgtt tcctcggtgc tgaactggat cagccggcca aaatacgcct
      901 cggtggaacg ccgggatgtc ccggccgggt tgtggaccaa gtgcccccgg tgcgccgccc
      961 tcgtgtaccg caaggaactg gagcgcaacc tccgggtctg cccccgctgc acctaccacc
     1021 atcggctctc cgccgccgac cggctggcga tggtgctcga cagcggatcg ttcgtcgaac
     1081 acgatgaagg attggtgccg gaagatcccc tggggttcgt ggacgagaaa ccttatcccg
     1141 cccgggtcga tcaggcccag cgccagaccg ggctgcccga ggccatcgtg accgggaccg
     1201 gcgcgatcga ggggcggcgc gcggtggtgg gcgcgatgga gtttgggttc ctcggcggca
     1261 gcatgggcgc ggtggtggga gagaaggtgg cccggagcgc cgagcgggcg ggctcgctgc
     1321 ggtggccgct ggtgctgttt tcggcctccg gcggcgcgcg gatgcaggaa ggcgccctct
     1381 cgcttctgca actggccaag acgagcgtcg cgctcggccg gctcgccgag gcggggatcc
     1441 catttctctc cgtcttgtgc gatccgacga cgggcggggt ggcggcgagc ttcgcgttcc
     1501 agggcgacgt catcattgcc gagccggggg cgctgatcgg attcgccgga cggcgcgtga
     1561 tcgagcagac cattcggcag aaactgcccg aggggtttca gaccgcagag tttctgctcg
     1621 agcacgggct catcgacgcg atcgtgccga ggggggacct gcgccccacg cttgggcggc
     1681 tgctccggct gtgcggcgcc ccgctggggc ccagcccctc cgattccccc caggcaaccc
     1741 cgacggaagg gcggtcagcc gatgatgagc gttctgcctg acccggcggg cgcggccggg
     1801 gcccgggacg acgcggcgga cgagcgtctc gagcgcgatc tcgcggaggc cgagcgggag
     1861 atcgccgagc tcaagcgcat caccgccacc acccagggca tggatatggc ccaccagatc
     1921 gccctgctcg aagcgcgcgc cgaggaactg cgggaggcga tcgccagcaa ccccacgccc
     1981 tggcagaccg tgcaactcgc ccgccacgcg gagcggccga agatcggcga ctacatcgcc
     2041 gcgatcacga ccgatttcat cgagctccac ggcgatcgga cgtttcggga cgacccggcg
     2101 atcgtggccg gcctgggctt tgtggacggc cggaccgtgg cggtgatcgg gcacgccaag
     2161 ggccgcgata cccgggagaa catcgcacgg aacttcggga tgcccaaccc ggagggatac
     2221 cggaaggccg tgcggatcat gcacctagcc gagaaactcc gcgcgccggt cgtcacgctg
     2281 atcgacaccc caggggcgtt cccgggcagg gaagccgaag aacgggggca gagcgaggcc
     2341 atcgcccgcg ccctcatcgg gatgggaagg ctccgcaccc cggtcgtcac cgtgatcacc
     2401 ggtgaggggg gcagcggcgg cgcgctggcg atcggggtcg gcgacgtcgt cctgatgctg
     2461 gagcgagcga tctactccgt gatctctccc gagggatgcg cggcgatcct gtggcgggac
     2521 gcctcccggg ggcgcgaggc ggcccgggcg ctccgcctca cagccccgga tctggtggcc
     2581 ttcggcatcg tcgacgagat catcccggaa cctccggggg gcgcccaccg gcaccccgac
     2641 cgggcggcgg ccgcagtcgc cgcggcggtc caccgccacc tcgccgcgct tgcggacagg
     2701 ccggtggagc tcctcctcga ggcgcgctat gcgaagttcc gccggatcgg ccgcctccgg
     2761 gatgccggcc aggtgccggg gcggtccccg agcgagcaca ccgcgtccga gtaggcggca
     2821 ccgcagcgaa gcgacccgga tggcgtgcga gccaggaggg ggagggggat gagccaagag
     2881 cgacggctgg atctcgagga gattcgggcg ctgatccgtc tggcgaccga agccgatatc
     2941 accgaactcg atgtcgatgc tccccacgtc aaggtgcgga tcaagaaggc cgcccgaaac
     3001 cccgtcgagc cgctcgcggc caaggtcccc cgcccgggcg gcgccatccc ggaggcggcg
     3061 tccgctgcgt cggagggagg cctggtcccg atcaccgcgc cgatggtcgg cacgttttac
     3121 cgcgccccca agcccgacgc ccctcccttc gtcagcgagg gggacgaagt ccgcgtcggg
     3181 caaacggtct gcatcctcga agcgatgaag ctgttcaacg agatttccag cgatgtgggc
     3241 ggccgcatcg cgcgggtcct ggcggagaac ggcgccccgg tggagtatgg acagccgctg
     3301 ttcctcgtcg accccgcggt ctaacccgta gtccgtcttg gcgacgttct ccaaggtcct
     3361 gatcgccaac cgcggcgaga tcgccgtccg gatcatccgc gcgtgccggg aattggggct
     3421 gagcacggtg gcggtgtact ccgaggccga ccgcggcgcg ctgcacacgc ggctcgcgga
     3481 cgaagccttc tgcatcggtc cggcgcccgc ccgcgagagc tacctcaaca tccccagcat
     3541 catcagcacg gctgaactgc tgggcgtcga cgccatccac ccagggtacg gctttctcgc
     3601 cgagaacccc cattttgccg aggtctgcag ggactgcggc atcaccttca tcggcccgtc
     3661 acccgaagcc atcgagaaga tgggcaacaa gtcggccgcc cgggagatgg tccgcaaggc
     3721 cggcgtcgcc atcgtgccgg gcagcgacgg gcccgtgcgc gacgaagcgg cggccgccgg
     3781 cgtcgccagg agcctcggct atccgctcat catcaaggcc gccggcgggg gcggcggccg
     3841 gggcatgcgc gtggtacaca accgcgacga tctgcgccgg gccctgaccg aagccagggg
     3901 cgaggccgag gcagcgtttg ggaacaccga ggtgtacgtc gagaagtacc ttgaagaacc
     3961 gcgccacgtg gaggtgcagg tcctcgcgga cacccgcggc accgtgatca cgctggggga
     4021 acgcgactgt tcggtccagc ggcgtcacca gaagctcatc gaggaatcgc cgtcccccgg
     4081 ggtcgggccc aagctgcgcc gggtcctcaa ccgcgcggcg gcccgggtgg cggaagcgtc
     4141 ggggtacacc aacgccggga ccgtggagtt cctcgtggat caggcggagc ggttctactt
     4201 tgtggagatg aacacccgga tccaggtgga gcacggggtg accgagatgg tcaccggcgt
     4261 cgacctggtg aaagagcagc tccggatcgc tgcgggggag cggctggcga tcccgcggga
     4321 ggtggaaccg cgggggcatg cgatcgagtg ccgggtgaac gcggaggatc cggcccgcga
     4381 cttccttccc tcgcccggcc agatcgtcgc gttcgtgccc cccgggggac ccgggatccg
     4441 ggtggatacg cacgcgtttg cgggctacac ggtgcccccc cactatgatt ctctgatcgc
     4501 gaaggtcatt gcctggggac aggatcgcga tgaggccatc acgcggatgc agcgggcgct
     4561 gggggagttc gaaatcacgg gcgtccaaac cacgatcccc ttccaccagg acgcgctgga
     4621 caacgcgttc tttcgtcggg gggaggtgta cgtgaacttc gtgcagcgcc ggatggatct
     4681 caccgccctc cgcgcctgac cccgggaccg ctgggcatgc gcggggcgat caccatcccc
     4741 ctccccaccc cgttcagcgt gccccgcacg ctgtcgtttc ttcggacctc gtccctccgg
     4801 acgccgtatc acttcgtcgg agcgcaccgg gtccggcggc tcgtccggct ggatggccgg
     4861 ccctcggtgg tcgagttcgg cttcgccccg cccggcgccc ggctgcgggt gcgcgcggtt
     4921 ggtccggtcg aggggcccgg cctggcctcc gccctccggc gccttgcgac gtcggtgtgg
     4981 agcctcgacg atgatctccg gcgctgctac gctgcgctga ggcgcgaccc cctcatggcg
     5041 ccgctcttga agcattgcgc cggcctccgc atgatccgga cgccggatct gtacgaggcg
     5101 ctgctcatcg cgatcatcgg gcagcaggtc agcgtggcgg cggccgagtc gattcgccgg
     5161 cgcctgatca ccaccctggg ggatcgggcc gtgcgcgagg ggatgaccta cgccgggtac
     5221 ccgccccccc ggcggctctt gagaacgtcc ccggccggcc ttcgcgcggt cgggctcagc
     5281 cggcagaagg cgcggtacgt gctggaaatc gccgaccgcg aggtggcggg ggttctcgcc
     5341 cccgccgcgt tcgacggcct ccctgacgaa caggcgatcg cgaggctcat cgagatcccg
     5401 ggcgtgggcc ggtggaccgc cgagatcgtt ctgatgcgcg gactgggccg cccagatgtg
     5461 tttcccgcgg cagacctggg gctcgggtta gcggtgcagc ggctgctcgg acgccggacc
     5521 cgccccacgg aagatgccct tcgcgccctg gccgaccggt ggatgggctg gcggagttat
     5581 ggcgcgctgt acctctggag gtcgctgggg atcgccgcgt aggcgcgggc gaggcgccgg
     5641 cctcctcacg cttctccgac tgagcgccca ccggccgcgt ggtacaccaa cgccgcggcc
     5701 ccgatgatcc cgacgtcgtc ccccaggtct gcgggcacga cctccacccc cagcgcgggg
     5761 gcaggataga cgcgggcccg catcgcgtcg cggaggggcc gaaacagcag gtccccggtc
     5821 ttgctcaccc cgccccccac gatgatcatc ttcggggtga ggagggccag gaggttgctc
     5881 agcacgagac cgatccgggt gccggcccgg cggtagagct ccgccgcgag cgcatcgccg
     5941 cccctcgcgg cctcggcaac ggactcggcg gtgatctcct cgggatgagc ggccaggcgc
     6001 cccagcgcgg tcggctctcc ccgcgccaca gcctcccggg cctgcctcgc gatcgcggtg
     6061 cccgaggcca ggacctccag acagccgcgg ttcccgcagt ggcagcgggg gccatccggc
     6121 tccacgatga tgtggccgat ctctcccgcc gtgccgttgg ccccccggta caggcgtcct
     6181 ccaagaatga gcccgccgcc gatgcccgtg gagaccgtga tgtacacgag atcccgaacg
     6241 ccgcggccgg cgccggtcca cgcctccccg agcgcggccg cgttggcatc gttctccacg
     6301 tagaccgtct ccccgagttc gcgcgcgagg agcgggccga aggcggtctc ccgcccccag
     6361 cccgtaaggt tcggcgggtc gtagacgacc ccgcgatcgt gatcggtcgg tgcggcgaac
     6421 gcgacgccga cgccagcgag gctcgcgccg gcagtcccgg cggccccccg ggggagcgag
     6481 cgggcgaggt cccggatcgc ccgggcgacc ggttcgggcc ccccttgggg cggcgcaatc
     6541 cgctggcggg ccaggacgcg cccgtcgcgc cccacgacgc cgaccaggag cttggtcccg
     6601 ccaagatcga ccgcgagcgc tggggcgttc atctccgcgg acgtcacagg agcgcgatct
     6661 cctctcgcct cgccttcacc agggctgtgg ctgcgcgggc acaggttcgg cgccgcggcg
     6721 tgcccttccc gccggggccg cgccgtcgac gccgcaagac cgcggggcgt cccgctcagg
     6781 gggagaggaa gcggcagggg gggatttccg tggaggcgaa ggggtcctcg gataggaggg
     6841 gttgagacat ggtggggtac gtgcttgccg tcttgatcgt cgcggtgctc ctcttgtgga
     6901 acggcatcaa gatcgtccgc gagtaccagc gcttggtggt gttccgcctg ggccgcagct
     6961 tcggcccaag gggtccgggg atcgtctacc tgatcccgat cgtcgacaag gccgtctggg
     7021 tggacctccg ggaagtgtac ctcgagatcc ccgcgcagac gtgcatcacc aaggacaacg
     7081 cgccgatctc cattgatttc ctaatctact ggaaggtcgt tgacccgcag ctcagcgtga
     7141 tccaggtcgg gaactttgcc ggcgcggcgc aggggatcgc caccaccggg ctgcgggccg
     7201 tgatcggcga catcagcctc gacgatgtcc tcgcgaagcg ggatcagatc aaccaggtcc
     7261 tgcgcgccaa actcgacgag gtcacggaac ggtggggcgt caaggtcacc acggtggaga
     7321 tccgcgagat caccccgccc aaggacgtcc aggaagcgat gacgcggcag atgtccgcgg
     7381 aacggagccg gcgggcgatc gtcaccgagg ccgacgggaa gaagcaggcc gcgatcacca
     7441 tcgccgaagg ggagaagcag gcggcgatcc tcaaagcgga gggcgaccgt caggcggcaa
     7501 tcttaagagc ggagggcttt gcgctggccc tcggcaagat cttcgaggtc gcgaggaccg
     7561 tcgacaccaa cacgatgagc ctgcagtacc tcgaggccct gaaggcgctc ggcgcgggcc
     7621 cggcgaccaa gtttgtgttc ccgatggagt tcaccaaact gctccagccg ttcaccgact
     7681 ttgccgggcg ggcgaccccg cctccgccga agacgccgta accagcccgg cggcgtagag
     7741 caagaaacac ctcgtgcagc agccaggtag cgacaaaact accacagccg tcaggaggag
     7801 gggcatatgg atgcccaagc ggttggcgac atcacccggt tcctatgggg gccgccagtc
     7861 gtggtgacca gtgcctggca cggcgagcca acgccatggt agccgccacc gccatgcggg
     7921 cgttcatcat ccccgaccgg ccacgcatca tcgtcctcat ctggaagccc aactacacgc
     7981 acagactcat agagcaaagc cgcgcctttg ccgtccatct ccttggcccc gagcaactgc
     8041 accaggtgcg agccttcggt ttgactccgg gcaggagcgc gacaaattga ccggagtggc
     8101 ttatgagacg aaagtgacag gaagcccgat actcacggac gcccttgctt acgtggactg
     8161 tcgcgtcgtt agcagcatgg acggaggcga tgtgacctgc tatcttgccg atgtggttga
     8221 cggtgcaagg gttcggcccg gcgaagtgat gacgtacagc atgtggcagg caaacatccc
     8281 accagaatgg aggcctcagt atgaggccat gctggcgaga caggtagccg tgtccccggc
     8341 ctatttggag agtcagcgcc ggggctaagg cttgcagaca cgtacactag acaaggcgac
     8401 gccccgtctc tccgacccga aacgagccat gcgctggctt cgggcgcagt atcaaagacg
     8461 ggctgaggaa gccgcctcca gccaagaccg catgatcgag cgcatcagga agtcaaacta
     8521 tacgaaggcc cctcattgag cagtcacgcg tagggcctct tgtctctagc cttgccgtgg
     8581 ctgcggagta gccttttggg gtttcctccg cccgagtctc tcgccaggga acgtgggtct
     8641 tcgaggaggt acagaagacg aagcagagcc gacgcacagt aatgttgcca gccttcgctc
     8701 aggacgcgct aggggctcag ttgacctgcc aggcgcacga gatgcccggc caggacgccc
     8761 cagcagtcga ggcgctggac cggatcatcg gggagattag cagagattag caaatt
//
LOCUS       DASQGH010000578         7544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50625, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000578 DASQGH010000000
VERSION     DASQGH010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50625"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VEW91_11485"
     CDS             complement(<1..831)
                     /locus_tag="VEW91_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYM92244.1"
                     /translation="MRIARVWVPGQGPRLGLCENGTVHLLASPDAPVLDSFEALAQVA
                     HRTRRRLTEAVQHFRGEGPAYSYRDLDRAPSPEAPHLLLPVVPPEVWAAGVTYERSRE
                     ARLAETTTVGIYDKVYEAERPELFLKATASRCVGPNVPIGIRADSRWTVPEPELGVVL
                     GTGGKVLGYTLGNDMSARDIEGENPLYLPQTKIYRACCSLGPTVLVTDEPVERRLTLW
                     CRITRGTREIFRGEVSTARIRRPISELIAYLRRSNDIPPMTVLLTGTGIVPPDEFACE
                     E"
     gene            complement(917..1777)
                     /locus_tag="VEW91_11490"
     CDS             complement(917..1777)
                     /locus_tag="VEW91_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYM92245.1"
                     /translation="MPEATKSIWEQNATPRHRIQWCPGCGDFAVLNALKAALTALELT
                     PSEVLLVGGIGCSGQIRNYLNGNSFYGTHGGALAYALGAKMANPELTVLALAGDGDTL
                     AIGVENFVHICRRDPDITLVIMDNGVYGLTKGQDSPSAGLGRPAAVSTEEHPPFLDPV
                     KLALVCGATFVAQSFSGDPKHATEMYVEAVRHKGFAMVNDFSPCVTYNKFNTYDWFRA
                     HCEPVPERHDPASEDAAWELIRDSERRGKLPLGVIYRHPRAKVPAKRLPMRQNELGRA
                     DLEPMLQGLR"
     gene            complement(1770..3644)
                     /locus_tag="VEW91_11495"
     CDS             complement(1770..3644)
                     /locus_tag="VEW91_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYM92246.1"
                     /translation="MTVNRMKLLVGGVQLRDGVSTITDLVGRILTRAGLHVVALERGF
                     ASTIYGAHQYDPLLITAEPAVSWGDESIDVLVGLEYDVDPDVPEQPNRDTIYRHGRNL
                     VDGGLLLYDSSSGPIQVEKLEARGARVFPIPARQIATRDLKREVVKNVVMTGALFRLI
                     EFDQDEAWLRQFLEERFGRKGPELVTLNLEAARRGRAAVEAILTERGWANVGYRVEPL
                     PTSAPAVYIGGNEALVIGAIEAGVRFYAGYPITPASSILEFMERHLPRYGGRALQGSN
                     ERESIRAALGASAAGALSMIGTSGPGLSLKVEEFGVAGVTETPLVIVDAMRAGPSTGM
                     PTKSEQGDLFLATGGGHGEIPRIVLAPASIEECYILIHEAVRLAETYQCPVFFMTDLN
                     LGEGRMTVPQAVFRSGRGAVERHLATEAEVRESRYLRYRLTDSGISPRLIPGTRGGVT
                     KMNSTEHDEFGFVTTEPSKRVAMMDKRMRKMTTYLHHDAKPPQVYGHPGKGPLLVGWG
                     STRAVLLDAQKRLQAEGIQAAVAHFTHLWPFPTHLAKPVLEAGRPVIVCEHNYLGQLA
                     EIIQAHTLIPARRVLKYNGRPFYPSEVVRAVHEVAHNGARVVRLGGREPVMVEVSTDA
                     "
     gene            complement(3878..4213)
                     /locus_tag="VEW91_11500"
     CDS             complement(3878..4213)
                     /locus_tag="VEW91_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYM92247.1"
                     /translation="MPPTKTLLPKRNSTSLEREGCMTKLQFATRLAEKLKVSKAEGGR
                     IVEEMTGLLTATLKKGDRVRFPGFGTFKVSKRKARIARNPQTGEPVRVPARTVPRFTA
                     AKELKAAVK"
     gene            4468..5001
                     /locus_tag="VEW91_11505"
     CDS             4468..5001
                     /locus_tag="VEW91_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92248.1"
                     /translation="MRTIVAALLVLGLAAVAVPVSASEVDVPAGTSVPLKFLEPLDSA
                     TIKEGTPVKFEVAADVVIQRSVIFRQGVHAQGLVTDVSQPGIFGTNARVHIAFIQVLA
                     SDGRPVRLSPLDVTPDSIKQVSDVGAAAGSSIAGAILLGPIGLAAGALIHGGQVSVPA
                     GAVGMTKVTESFRAEVQ"
     gene            complement(5005..6687)
                     /gene="ilvD"
                     /locus_tag="VEW91_11510"
     CDS             complement(5005..6687)
                     /gene="ilvD"
                     /locus_tag="VEW91_11510"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYM92249.1"
                     /translation="MARATTDLRQRSRNVTEGPERAPHRAMLRATGLRDEDLKKPQIG
                     VASSWNEVTPCNLHLDVDAKDVKGGVRAANGTPIQFTTIAVSDAIAMGHEGMKASLVS
                     REVIADSVELMVLAEAFDGLVAIAGCDKSLPGMMLAIARLNVPAIFLYGGTIMPGKFD
                     GRDVTITDVFEAVGAHAQGRMTDQDLYRLECVACPGAGSCGGLFTANTMASCVEALGM
                     SLPGSASVPALDPRRPELCRRTGEAAMRLLTEGIRPRDILSREAFENAITALVAMGGS
                     TNGVLHLLAIAGEAGVPLALADFDRISRQTPHIADMRPGGRYTMADLDRVGGMPVVLR
                     ELLDGGYLHGNALTVTGRTLAENLREVRRPGGQDVVRSVRDPITPSGTLAILSGSLAP
                     DGAVIKTAGVHRLTFRGPARVFDREEDAFDAVVHNRLSRDDVLVIRYEGPKGGPGMRE
                     MLAVTGGLFGQGLGEEIALITDGRFSGGSHGLVIGHVAPEAAHGGPIAAVREGDIISI
                     DVPQRKLDLELPAEEIQSRLRLWRPPQPRYRSGALAKYARLVSSSALGAVCQ"
     gene            6825..6968
                     /locus_tag="VEW91_11515"
     CDS             6825..6968
                     /locus_tag="VEW91_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92250.1"
                     /translation="MIRLVCPHCHVPFLRVMPAEDGEEVVCPGCKQPFVPEEEEWVDP
                     EDE"
     gene            complement(6972..>7544)
                     /locus_tag="VEW91_11520"
     CDS             complement(6972..>7544)
                     /locus_tag="VEW91_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92251.1"
                     /translation="AQDGALYISDGYGNSRIHKFSPDGKHLFSWGEPGTDPGQFSLPH
                     NICADREGYLYVADRENHRIQVFDQRGRFEAQWNNLFRPCGLYIDQRDPSGERVYVGE
                     LGPSLPVSKGVPNLGARVGIYTLDNHLLARLGDRLPGIGPQQFLAPHCLAVDSSGAVY
                     VGEVSWTEYGRNLTPPQEVRSFRKLVRVAQ"
BASE COUNT         1138 a         2657 c         2533 g         1216 t
ORIGIN      
        1 ttcctcgcac gcaaactcat cgggggggac gatcccggtt cccgtcaata ggaccgtcat
       61 cggcgggatg tcgttcgatc gccggaggta ggcgatcagc tccgaaatcg gccgccggat
      121 gcgcgccgtg ctgacctccc cgcgaaagat ctcgcgggtc ccgcgtgtga tgcggcacca
      181 gagcgtgagg cgcctctcca ccggctcatc ggtgacgagc accgtcgggc cgaggctgca
      241 gcacgcgcgg tagatcttcg tctgcgggag gtacagcggg ttctcacctt cgatgtcgcg
      301 ggcgctcatg tcgttaccga gcgtgtaccc gagcaccttc cccccggtgc cgagcacgac
      361 gccgagctcc ggctcgggga ccgtccagcg ggagtccgcg cggatcccga tcgggacatt
      421 cggtcccacg catcgggacg ccgtggcctt gaggaacagt tccgggcgct ccgcctcgta
      481 gaccttgtcg tagatgccga ccgtggtggt ctccgccaag cgcgcttcgc ggctccgctc
      541 gtacgtgacg ccggcggccc agacttcagg ggggaccacc gggaggagca aatgcggggc
      601 ctccgggctc ggggcgcgat ccaggtctcg gtatgaatac gccggcccct cgccccgaaa
      661 atgttgcacg gcctcggtga gacggcgccg ggtgcgatgc gcgacctgtg ccagcgcctc
      721 gaacgagtcc aggacaggcg catctgggga cgccagcagg tggaccgtgc cgttctcgca
      781 cagccccagg cgcggcccct gcccgggcac ccacacccgg gcgatgcgca ctccgctccc
      841 tcccgcggct cggtgctggc tcactacgga tcggttctcg cccgcggtcg gcgacccctc
      901 gcccgggggg cttgggttag cgcaacccct ggagcatcgg ttcgagatcg gcccgcccga
      961 gttcgttctg ccgcatcggg agccgcttcg cgggcacctt cgccctcggg tggcggtaga
     1021 tgaccccgag ggggagcttc ccgcgccgct cggagtcgcg gatgagttcc caggccgcgt
     1081 cctcgctcgc gggatcgtgg cgctccggca ccggctcgca gtgggcccgg aaccagtcgt
     1141 acgtgttgaa cttgttgtag gtcacgcacg gcgagaaatc gttcaccatc gcgaacccct
     1201 tgtggcgcac cgcctcgacg tacatctcgg tcgcgtgctt ggggtcgccc gagaacgact
     1261 gtgccacgaa cgtcgccccg cacaccaatg ccagcttcac cggatcgagg aagggcgggt
     1321 gctcctcggt ggacaccgcc gccggcctcc cgagccccgc cgagggggag tcctgcccct
     1381 tggtgagtcc gtacaccccg ttgtccatga tcacgagcgt gatgtccggg tcgcggcggc
     1441 agatgtggac gaagttctcc accccgatcg ccagcgtgtc tccgtcgccg gccagggcca
     1501 gcacggtcag ctcggggttc gccatcttgg ccccaagcgc gtaggcgagc gcgccgccgt
     1561 gcgtgccgta gaacgagttc ccgttgaggt agtttcggat ctgcccggag cacccgatgc
     1621 cgcccacgag cagcacctcg gacggggtca gctcgagggc cgtgagcgcg gccttgaggg
     1681 cgttcagcac ggcgaagtcg ccgcacccgg ggcaccactg gatccggtgc cggggggtgg
     1741 cgttctgctc ccagatcgac ttcgtggcct caggcatcgg tgctcacctc gaccatcacg
     1801 ggctcccggc cgcccaggcg caccaccctc gcgccgttgt gggccacctc gtggacggcc
     1861 cggaccacct cggacgggta gaacggtcgg ccattgtact tgagcacccg gcgggccggg
     1921 atcagggtgt gcgcctggat gatctcggcc agttgcccga gatagttgtg ctcgcagacg
     1981 atcaccggcc tcccggcctc caggaccggt ttggccaggt gcgtcgggaa cggccagagg
     2041 tgggtgaagt gcgccaccgc ggcctggatg ccctcggcct gcagccgctt ctgggcatcc
     2101 agaaggaccg ccctggtcga gccccacccg acgaggaggg ggcccttccc cgggtgcccg
     2161 tagacctggg gtggcttggc gtcatggtgg agatacgtgg tcatctttcg catccgcttg
     2221 tccatcatgg ccacgcgctt cgagggctcg gtggtgacga acccaaactc gtcatgctcg
     2281 gtgctgttca tcttggtcac cccgccccgc gtgccgggga tcagccgggg ggagatgccg
     2341 gagtcggtga gccggtaccg caggtagcgg ctttcgcgca cctccgcctc ggtggccagg
     2401 tggcgctcca ccgccccccg gccagaccgg aagaccgcct ggggcacggt catgcggcct
     2461 tcgccgaggt tgaggtcggt catgaagaac accggacact ggtaggtctc cgcgaggcgg
     2521 accgcttcgt ggatcaggat gtagcactcc tcgatcgacg cgggcgcgag cacgatcctg
     2581 gggatctcgc cgtggccgcc cccggtcgcc agaaaaagat ccccctgctc ggacttggtg
     2641 ggcatcccgg tcgaggggcc cgcccgcatc gcgtcgacga tcacgagcgg ggtttcggtc
     2701 acccccgcga ccccgaattc ctcgaccttg agcgaaagcc cggggccgct cgtcccgatc
     2761 atgctgagtg cgcccgccgc gctcgcgccc agggccgcgc ggatggactc gcgctcgttg
     2821 gagccctgaa gggctcgccc gccgtaccgc ggcaggtgcc gctccatgaa ttcgaggatc
     2881 gatgatgcgg gcgtgatcgg ataccccgcg tagaagcgca cccccgcctc gatcgcgccg
     2941 atcacaagcg cctcattgcc gccgatatag accgccggcg ccgaggtggg caggggctcc
     3001 acgcggtagc ccacgttcgc ccagccgcgt tcggtgagga tcgcctcaac cgcggcccgt
     3061 ccccgccggg cggcctccag gttgagggtc accagttccg gccccttgcg gccgaaccgc
     3121 tcctcgagga actgacgcag ccacgcctcg tcctggtcaa actcgatgag gcggaagagc
     3181 gcaccggtca tgacgacgtt cttcacgact tccctcttga ggtcccgggt ggcgatctgg
     3241 cgggcgggga tcgggaagac gcgcgccccg cgggcctcga gcttctcgac ctggatgggg
     3301 ccggaggaac tgtcgtagag gaggaggccg ccgtccacca ggttccggcc gtgccggtag
     3361 atggtgtcgc ggttgggttg ctcgggcacg tccggatcga cgtcgtactc cagacccacg
     3421 agcacgtcga tggattcgtc tccccaggac acggcaggct cagcggtgat caacagcggg
     3481 tcgtactggt gcgcgccgta gatcgtcgag gcgaaccctc gctccagggc cacgacgtgc
     3541 aaccccgccc gggtgaggat ccggccgaca aggtcggtga tcgtggacac gccatcccgg
     3601 agctgcactc ccccgacgag cagcttcatc cggttcacgg tcataggcga ctgtgtgcct
     3661 cctgagagcc gctggatggc tgcacgccgg ggcgggaaaa cacccatcgc gcccgagcgt
     3721 cgcggctcga gccttccgaa ggcttatagt accatatttt gagcccggac cgccacgcca
     3781 accaggctcg ccgcagcacc ggcaggcggg ccaaagccag ccgagtccgc gccctcagcg
     3841 gggcgcggac ccggcgccaa ccaatggaag atcggggtta tttcaccgcg gctttgagct
     3901 ccttcgcggc ggtgaacctg ggaaccgtcc gtgccggcac gcgcaccggc tcacccgtct
     3961 gcgggttgcg cgcaatccga gccttccgct tggacacttt gaatgtgccg aaacccggga
     4021 accggacccg atcgcccttc ttcagggtcg ccgtcaggag cccggtcatt tcctcgacga
     4081 ttcgacctcc ttccgccttg ctcaccttga gcttctcggc cagcctcgtc gcgaactgta
     4141 acttcgtcat acacccctcc cgctccaggc ttgtggagtt cctcttcggg agtagggtct
     4201 tcgtgggagg catgccgttt ccttctgcag gccgcatgaa aactggattt tctgcggaca
     4261 cgcgtacggg gccggggata ggcgcacgtc cggtcgaatg tccagcgcga ccgcgcgccc
     4321 ggggcggccg gcgcgtcgcc accgtccacc aagggaaaat cgcccgcagc gcgaatctgt
     4381 cccagggacg aggtccggag gcgatcaacg caccagaggt ggcgctacca cacccatgat
     4441 ctcgatcgga gaggaggaaa cacgccgatg cgcaccatag ttgcagctct tctggtccta
     4501 gggcttgccg cagtcgccgt tcctgtcagt gccagcgagg tcgacgttcc ggccggcacc
     4561 tccgtcccgc tgaagtttct cgagccgctg gactccgcga cgatcaagga gggcaccccg
     4621 gtcaagttcg aggtagccgc cgacgtcgtg atccagcgat cggtgatctt tcgccagggc
     4681 gtgcacgcgc aggggctcgt gaccgacgtc tcgcagcccg gcatcttcgg gacgaacgcg
     4741 cgcgtccaca tcgccttcat ccaggtgctg gccagcgacg ggcggccggt ccgcctgtcg
     4801 ccgctcgacg tgacgcccga ctccatcaag caggtctcgg acgtcggcgc ggcggccggc
     4861 tcgagcattg ccggggcgat cctgctcggc ccgatcgggc tcgcggccgg cgccttgatt
     4921 cacggggggc aggtgagcgt ccccgccggg gccgtcggga tgaccaaggt caccgagtcg
     4981 tttcgcgctg aggtgcaata ggctctactg gcacaccgcc ccaagcgcgg acgaggacac
     5041 gagtctggcg tatttcgcga gcgcccccga gcggtagcgc ggttgggggg gccgccagag
     5101 ccgaagccgg gactggattt cctccgccgg gagttcgagg tcgagtttcc gctgggggac
     5161 gtcgatgctg atgatgtccc cctcgcggac ggccgcgatc gggcccccgt gcgcggcctc
     5221 gggggcgacg tggccgatca ccagcccgtg ggaccccccc gagaagcggc cgtcggtgat
     5281 gagcgcgatc tcctccccca gcccttgtcc gaagagaccg cccgttaccg ccagcatctc
     5341 gcgcatgccg ggtccccctt tggggccctc gtaccggatg accaggacgt cgtcgcggga
     5401 gaggcggttg tgcacgacgg cgtcgaacgc atcctcttcc cggtcgaaga cccgggcggg
     5461 accgcgaaac gtcagccggt ggaccccggc cgtcttgatc accgccccgt cgggcgccag
     5521 ggatccactg aggatggcca gcgtcccgct cggggtgatc gggtcgcgca ccgaccggac
     5581 gacgtcctga ccgccggggc ggcggacctc ccggaggttc tcggcgagcg tccggcccgt
     5641 cacggtgagg gcgttcccgt gcaggtatcc cccgtccagc aactcgcgca gcacgaccgg
     5701 catcccgccc acccggtcaa ggtcggccat cgtgtaccgg ccgcccggac gcatgtcggc
     5761 gatgtggggg gtttggcggc tgatgcgatc aaagtcggcc agggcgagcg ggacccccgc
     5821 ctcgccggcg atcgccagaa ggtggagcac cccgttggtg gaccccccca tggccacgag
     5881 cgcggtgatc gcgttctcga acgcttcccg cgacagaata tcccggggcc ggatcccctc
     5941 tgttagcagg cgcatcgcgg cctcaccggt ccgccggcag agctccgggc ggcggggatc
     6001 gagcgccggg accgacgcgc tcccggggag cgacatcccg agcgcctcga cacaggacgc
     6061 catcgtgttg gccgtgaaga ggccgccaca ggatcccgcg cccgggcagg ccacacactc
     6121 gagacggtac aggtcctgat cggtcatccg tccctgggcg tgcgccccca ccgcttcgaa
     6181 cacgtccgtg atcgtcacgt cccgcccatc gaacttgccg ggcatgatcg tcccgccgta
     6241 caggaagatc gcgggcacat tgagccgggc gatcgccagc atcatccccg ggaggctctt
     6301 gtcgcacccc gcgatcgcca cgaggccgtc gaacgcctcg gccagcacca tcagctccac
     6361 ggaatcggcg atgacctcgc ggctgaccaa ggacgccttc atcccctcgt ggcccatcgc
     6421 aatcgcatcg ctcaccgcga tggtcgtgaa ctggatgggc gtgccgttcg ccgcccggac
     6481 gccccccttg acgtctttgg catccacgtc gaggtggagg ttgcacgggg tcacttcgtt
     6541 ccacgaggac gcgacgccga tctgcggctt cttgagatcc tcgtcccgca gaccggtcgc
     6601 ccgcagcatc gcccggtggg gcgcacgctc cggcccttcg gtgacgtttc gcgatcgctg
     6661 cctgagatcc gtggtcgccc gggccatcag aacgcgcctc ctgggatcac ctgcccccgc
     6721 gaaatgcggg gctgtgcgga atgagtgacc gctccgcttc tccgccctgg atggtgagtc
     6781 ctgccgagga ctcgtccgcg cacccgccga agggctcgtg cgggatgatc cgcctcgtct
     6841 gcccgcactg ccacgtgccg ttcctccggg tcatgccggc ggaagacggc gaagaggtcg
     6901 tctgccccgg ctgcaagcag ccgttcgtcc ccgaagaaga agagtgggtg gatccggagg
     6961 acgagtagcg cctactgcgc cacccgcacc agcttccgga aacttcgcac ttcctgcggc
     7021 ggggtgagat tccgcccgta ctccgtccac gacacttctc ccacgtacac ggccccggac
     7081 gagtccacgg caagacaatg gggggccagg aactgctggg gcccgatccc gggcagccgg
     7141 tcccccagcc gcgccaggag gtggttgtcg agcgtataga tgccgacccg ggccccgagg
     7201 ttggggaccc ccttgctcac cggcaggctc ggccccaact ccccgacgta cacccgctcc
     7261 cccgagggat cgcgctgatc gatgtagagg ccgcacgggc ggaagaggtt gttccattgg
     7321 gcctcgaacc gtccccgctg atcaaacacc tggatgcggt ggttctcccg atcggcaacg
     7381 tacagatacc cctcccgatc ggcacagatg ttgtgcggca ggctgaactg cccgggatcg
     7441 gtgccgggtt ctccccacga gaagagatgt ttcccgtccg gcgagaactt gtggatccgc
     7501 gagttgccgt agccgtcaga aatgtacaac gcgccgtcct gggc
//
LOCUS       DASQGH010000579         7957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47886, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000579 DASQGH010000000
VERSION     DASQGH010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47886"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VEW91_11525"
     CDS             <1..1426
                     /locus_tag="VEW91_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92252.1"
                     /translation="MVIPLPLPLREVAVAGAGVAVVSAPLLVWLLRPSLLGAARLADR
                     RLGLADRLSTALEGLAGPASPRGLGRLQVADAVEVVQGILPRSAAPIAFPREGWAALA
                     LVGAVVLWAQFLQGWTIPGLPAARNVAVIHKEGRTLVMIAQHLEAMSRTRGLPETRRA
                     APELQDLGRRLEGPRVTRPSALRMLQEAVRELRAAESRVERRLGGTAVRGAPGAGEAR
                     NPSLNPADPDRLAQTIRELESLTGQLRSEGGGSAREDLSRRLRAVSESLDQMNAPGAP
                     RRDVAKARREVERGRFGAAESALGDALQDLQGLERVLGDERALGEARRQVESAAERIA
                     QQGSSGAEVRETGQASPDSAPPTSPGTGPVAPTSEDTAPPPPGPNQGSLPGEGRGPRL
                     GAPIPRPEGSRVEEHLTGRQGEGTAITRDLLAAGRAGVPQLPASPVPAAVAHQNDRAL
                     AHEPLPPAYLTLIRRYFEALENGR"
     gene            1423..2433
                     /locus_tag="VEW91_11530"
     CDS             1423..2433
                     /locus_tag="VEW91_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYM92253.1"
                     /translation="MTDPQPRDVAEFRRTFAALRSELSRVIVGQEELLELVTIALLAG
                     GHVLLEGVPGLGKTLLVRTLAQALDLQFSRIQFTPDLMPADILGTNLVVQDDSGRRRF
                     EFHPGPIFSQIVLADEINRATPKTQSALLEAMQEHAVTIAGESRRLDEPFFVLATQNP
                     IEMEGTYPLPEAQLDRFLFKLRVPFPGLEDLQVIIDRTTGPEEPAAHQVADGATVVRM
                     QALARQVPVATHVREYAARLVLSTHPDSEGAVQAARRFVRYGASPRGVQALILAGKVR
                     ALSQGRLNVAVDDLRGLAFPALRHRLILNFEGEAEGTDTDAVLRAAVDELEAGEIPAP
                     EG"
     gene            2439..3353
                     /locus_tag="VEW91_11535"
     CDS             2439..3353
                     /locus_tag="VEW91_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYM92254.1"
                     /translation="MAPPVGLSPEGGRVRLLVPEFLQKLETLSLVSRRRFRGLQRGDR
                     RSTARGRGVEFDDHRAYHPGDDYRYIDWNVVSRLDRLFVKMFSEEEDLDISLLIDTSR
                     SMAAGHPPKLRLATQLAAAIGYIGLVNLDRVGVTAFASTNAARLDRLRGRARAFDLLE
                     FLERLEPAGDTDLGLALRRHLATSRRRGLLILLSDLFDPRGYEQGLLLCRHHRVQTFL
                     IHVLAEEELSPRLAGDLRLVDVETHQAVDVTVDAEALRAYAAARDAFFHGIERFCFRH
                     GIDYLRTTSSVSVETLALRWLRQGGLLQ"
     gene            3350..5149
                     /locus_tag="VEW91_11540"
     CDS             3350..5149
                     /locus_tag="VEW91_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYM92255.1"
                     /translation="MSLGAPAALWGLLAIPCLILLYLLRVRRRDHPVSSTLLWQRSAP
                     TLAAYRPLRRIERSLLLLLQILAAAAVVLALAQPSVIVRGGERGDLILVLDLSLSMRA
                     RDVSPTRFDRARGEALDLVAHLHPGQRSGIVVAGPRPRLLLPPTDDRARVSAALRALE
                     PYDAAGDLGGAVLMAAELPESSGRRIVAWTDAARAGLPALPRVTYRILGSSADNIGIT
                     MFRIARDPSGAEALLRLDNFGDRPRRVPLDVTHDGGTVYHEIVDVPAGGSRTVVFPVT
                     GSGEFRARADTHDILPEDDVAVAVLDPAPLPAVLLVSDGNPYLERVLRVLPVSRAATT
                     RSMDPSVWAAYGVVILDRITPGPLPPGDYLLIGAVPSNLPVSAAGDVRRPEFSTWDGT
                     DPVLQFVDLSSVRVSRSLALTAEGGRVLAGGEVPLLWAFEGGGIRALLLGFALQDSDL
                     PQHVAFPVLIANSLAWLGGGPLEIGVGEPLEIPSGAATSGELTDPAGRRQVVRATGGM
                     ILLPPLTRAGVYHLKTTAGTREITVRPAARPAGRIRPGVAPAATPGAGVPGPETRQPL
                     LRRVPVWPWFVLGAVAAMATEWALATRRRGGEP"
     gene            5146..7893
                     /locus_tag="VEW91_11545"
     CDS             5146..7893
                     /locus_tag="VEW91_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYM92256.1"
                     /translation="MIAPVAFARPWALGLLLLVFVALALGRRRLGIRWRVATILRAAT
                     LGLLVLALSGPLVRVPVPGVSVVFAVDRSRSITPEGARAETAFVGEALARMRSQDRAG
                     VVTFAGRPLLRVPVEARPAVEDLGPAQDPDATDIGAALDLAVRMLPDEGVRRVVVLSD
                     GAENRGDAAAAARVARQSGVVVDAVPIVSGTPQEVLVEEVAAPQEVHPGEVYEVRAVL
                     RATGPADAVVTLSRNGVATATRHLSLSPGTTAVPFSETATHSEVARYRVDVVASPATI
                     PENKHGEALVVVRDRPQVLFVTAGPTRLPQWLRDQGLRIDVHTPEEVPEHLVGFAPYG
                     SVVLNDVSALDLSQAQQESIRAFVETGGGGLVAIGGGHSYGVGGYAGTPLEDALPVTM
                     DVRQTLALPTVAIVLVIDASGSMEAFGTELAKEELAKEIASSVIDLLGEHDQIGVITF
                     DQEYRWLVPPTEARERQRVLEQIARLRAGGGTVMAPPLRAAGEFLHHSSAKIRHVIVL
                     SDGLTDPGDFRGIVAGMARDKITVSTVAIGRDADLEFMRNLARWGGGRTYVAKDLYSV
                     PKIFTAEAVMAVRSFVVEEPVSLTRSGAGPTLAGLTPPPPIRGYVATAPKPFADIALT
                     GPRGDPILASWQYGLGRTVAITSDDGVRWTAPWASWSDVARFWSQAVRWTLSDDASGL
                     HLATALDRSGTGGRAILHARRIDGAPWDGLDVRGDLVAPDGTHTAIVLEQTAPGRYEG
                     AVAAPGTGVYSLAILARDGQRNVGNRTVGFVVPYSPEYRLPGGNLALLSRVVETTGGA
                     LLARPEEAFRPGRGTGQRETWPPLAAAAVALLLAEVAVRRLPALGQRLAMALAVAGHW
                     GGRRQAPSPAVAASDHAYAAADRWAVEDARFAEEEALRAASMEQAARIFITRLRGTKR
                     P"
BASE COUNT          987 a         2849 c         2946 g         1175 t
ORIGIN      
        1 gatggtgatc ccgctgccgc tgccgctccg cgaggtcgcg gtcgcgggag ccggcgtcgc
       61 ggtggtgagc gctcccctac tggtctggct gctccggcct tcgttgctcg gcgccgcgcg
      121 gctcgccgat cgccgcctcg gcctggccga ccgcctgagc acggcgctgg aggggctcgc
      181 cgggcccgca tctccgcgcg ggttgggacg tcttcaggtc gccgacgccg tcgaggtcgt
      241 gcaggggatc ctcccccgct cggccgcgcc gatcgccttt cctcgggagg ggtgggcggc
      301 gctcgccctg gtgggggccg tggtcctgtg ggcgcagttt ctgcagggct ggacgatccc
      361 cgggttgccg gcggcgcgga acgtcgcggt aattcacaag gagggccgga cgctcgttat
      421 gatcgcccag cacctggagg cgatgagccg cacccgaggc ctcccggaga cccgccgggc
      481 ggctcccgaa ttgcaagacc tcgggcgcag actcgagggg ccccgggtga cccgcccgag
      541 tgcgctgcgg atgctccagg aagccgtccg cgagcttagg gccgccgagt cgcgggtgga
      601 gcggcgcctc gggggaacgg cggtgcgggg cgcccccggg gcgggggagg cccgcaatcc
      661 ttccttgaat ccggccgacc cggaccgact cgcgcaaacg attcgcgagt tggagtccct
      721 cacagggcag ttgaggagcg agggcggcgg gagcgcgcgg gaggatctct cgcggcggtt
      781 gcgggcggtg tcggagtccc tggaccagat gaacgccccc ggcgctccgc gccgcgatgt
      841 ggccaaggcc cgccgcgagg tcgaacgcgg ccgattcggg gcggcggaat ctgcgctcgg
      901 cgacgcgctg caggatctgc agggcctcga gcgggtgttg ggagacgagc gagccctcgg
      961 cgaggcgcgg cgccaggtgg aaagcgcggc cgagcggatt gctcagcagg gatcgtcggg
     1021 ggccgaggtc agggagaccg gccaggcctc gcctgactcc gcgccgccca cctcgcccgg
     1081 caccggcccg gtcgccccaa cgtcggagga tacggcgccg ccgccccccg ggccgaacca
     1141 aggcagtttg ccgggcgagg ggcgggggcc ccggctgggg gcgccgatcc cgcggcccga
     1201 gggctcccgg gtggaggagc acctgaccgg gcggcagggg gaggggacgg ccatcacacg
     1261 cgacctgctg gccgcggggc gcgccggggt gccgcagctt ccggcctctc cggtgccggc
     1321 ggcggtggcc caccaaaacg accgcgccct cgcccacgag cccctcccgc cggcctacct
     1381 caccctgatc cgccggtact tcgaggcgct ggagaacggg cgatgaccga tccccaaccg
     1441 cgcgacgtcg ccgagtttcg ccgcacattt gccgcactcc gatcggagtt gagccgcgtc
     1501 atcgtcggcc aggaggaatt gctcgagttg gtcacgatcg cgctgctggc gggcggacac
     1561 gtgctgctcg aaggcgtgcc ggggctcgga aagacgctgc tggtacggac cctggcccag
     1621 gcgctggatc tccagttctc gcgcatccag ttcaccccgg acctcatgcc cgccgacatc
     1681 ctgggcacga acctggtcgt ccaggacgac agcgggcggc ggcggttcga gttccatccc
     1741 ggccccatct tctcgcagat cgtgctcgcc gacgagatca accgcgcgac cccgaagacc
     1801 caatcggcgc tcctcgaagc gatgcaggag cacgccgtca cgatcgccgg ggagtcgcgc
     1861 cgcctcgacg agccgttctt cgtgctggcg acccagaacc cgatcgagat ggaagggacc
     1921 tacccgctgc ccgaagccca actcgatcgg ttcctgttca agctccgcgt gcccttcccg
     1981 ggcctcgagg acctgcaggt gatcatcgac cggaccaccg gaccagagga gcccgccgcg
     2041 caccaggtcg ccgacggggc cacggtggtc cggatgcagg cgctggcccg ccaggttccc
     2101 gtggcgacgc acgttcggga gtacgcggcc aggctggtgc tctcgacgca ccccgactcg
     2161 gaaggggccg ttcaggccgc ccgccgcttt gttcgctacg gcgccagccc ccggggggtg
     2221 caggccctga tcttggcggg caaggtccgg gccctcagcc agggccggtt gaacgtggcg
     2281 gtcgatgatc tccgggggct cgcgttcccc gccctccggc accgcctgat tctcaacttc
     2341 gaaggggagg ccgaagggac ggacacggac gcggtgctcc gggcggccgt cgatgagctc
     2401 gaggcggggg agatccccgc gcccgagggg taggggcgat ggcgcctccc gtggggctga
     2461 gtcctgaggg cggccgggtg cgcctgctgg tccccgagtt cctccagaag ctcgagacgc
     2521 tcagcctcgt gagccgccgg cggttccgcg gcctgcagcg cggcgaccgc cgcagcacgg
     2581 cccgggggcg cggcgtggag ttcgacgacc accgggcgta ccacccgggc gatgactacc
     2641 gctacatcga ctggaacgtc gtgtcccgcc tcgatcggct cttcgtgaag atgttctccg
     2701 aggaagaaga cctcgacatc tccctcctga tcgataccag ccggtcgatg gccgccgggc
     2761 acccgccgaa gctccggttg gccacgcagc tggccgccgc gatcggctat atcgggctcg
     2821 tcaacctcga tcgcgtgggg gtcaccgcgt tcgcctcgac gaacgccgcc cgcctcgacc
     2881 ggttgcgcgg ccgggcccgc gccttcgatc tgctggagtt tctggagcgg ctcgagccgg
     2941 ccggggacac ggatctgggg ctggcgctgc gccggcacct ggccacgtcc cgccgccggg
     3001 ggctgctgat tctcttgtcg gatctgttcg acccccgggg gtacgagcag ggcctgctgc
     3061 tgtgccggca ccaccgggtg cagacgttcc tgatccacgt gctggcagag gaagagttga
     3121 gcccgcgcct cgccggggac ctgcggctcg tggacgtgga gacccaccag gcagtggacg
     3181 tgacggtcga cgccgaggcg ctgcgcgcgt atgcggcggc ccgcgatgcg ttctttcacg
     3241 ggatcgagcg gttctgcttt cgccacggga tcgactacct gcgtacgacg tcgtcggtat
     3301 ccgtggagac gctggccctg cgctggctcc gccagggggg gttgctccag tgagcctggg
     3361 tgcgccggcg gcgctgtggg gactgctcgc catcccgtgc ctgatcctcc tgtacctgct
     3421 gcgggtccgg cgccgggatc accccgtcag tagcaccctc ctgtggcagc ggtccgctcc
     3481 caccctggcc gcctaccggc cgttgcggcg gatcgagcgg agcctgctgc tcctgctgca
     3541 gatccttgct gccgcggcgg tcgtcctcgc gcttgcccaa ccatcggtga tcgtgcgcgg
     3601 aggggagcgc ggcgacctga tcctggtgct ggacctgtcg ctctcgatgc gggcgcggga
     3661 cgtctccccg acccggtttg accgcgcgcg cggcgaggcg ctcgacctcg tcgcccacct
     3721 ccacccgggc cagcggtccg gcatcgtggt cgcgggtccc cggccgcgct tgctcctccc
     3781 ccccaccgac gatcgcgcca gggtgagcgc ggcgctccga gcgctcgaac cctacgacgc
     3841 cgcgggcgat cttgggggag ccgtgctgat ggcggccgag ctgccggaat cgtccggccg
     3901 gcggatcgtg gcctggacgg acgcggcgcg cgccggactg ccggccctcc cccgcgtgac
     3961 gtatcggatc ctggggagtt ccgccgacaa catcgggatc accatgtttc gcatcgcgcg
     4021 ggatcccagc ggggccgaag cgctgctgcg gctcgacaac ttcggcgacc gcccccggcg
     4081 ggttcccctc gacgtgaccc acgatggggg gacggtgtat cacgagattg tggacgtgcc
     4141 ggccgggggg agccggacgg tggtgttccc ggtcaccgga tccggggagt tccgggcgcg
     4201 cgcggacacg cacgatatcc tgccggagga cgacgtcgct gtggcggtgc tcgaccctgc
     4261 cccgctcccc gcggtcctcc tggtgagcga cggcaacccc tacctcgagc gggtcctccg
     4321 cgtcctcccg gtcagccgcg cggcaacgac ccggagcatg gatccgtcgg tgtgggccgc
     4381 ctacggcgtc gtgatcctcg accgcatcac gcccggcccg ctgccccccg gagattacct
     4441 gctgatcggc gcggttccgt cgaacctgcc ggtctccgct gccggagatg ttcgccgtcc
     4501 cgagttcagc acgtgggacg ggacggatcc cgtgttgcag ttcgtggacc tgagctccgt
     4561 gcgggtctcg cgttcgctcg cgctgaccgc ggaggggggc cgcgtgctgg ccggcggcga
     4621 ggtgcccctc ctgtgggcct tcgagggcgg cgggatcagg gcgctgctgc tgggattcgc
     4681 cctccaggac tccgatctcc cccagcacgt ggcctttccg gtgctcatcg ccaacagcct
     4741 ggcgtggctg ggcggcgggc cgttggagat cggggtaggg gagccgcttg agattccctc
     4801 cggcgcagcg acatcgggag agttgaccga tcccgccggc cgccgccagg tcgtacgggc
     4861 gaccggcggc atgatcctcc tcccgccctt gacgcgggcc ggcgtctacc acctgaagac
     4921 caccgcgggg acgcgggaga tcaccgtacg acccgccgcc cgacccgcgg ggcgcatccg
     4981 gcctggggtg gcgcccgcgg cgacccctgg ggcgggggtg ccggggccgg agacgcgcca
     5041 gcccctgctg cgccgggtgc cggtgtggcc gtggttcgtg ctgggagcgg tggcggcgat
     5101 ggcgaccgag tgggcgcttg ccacacgccg ccgcgggggg gagccatgat cgcccccgtg
     5161 gcgtttgcgc gcccctgggc cctcgggctg ctgctcttgg tgttcgtcgc cctggcgctg
     5221 ggacggcgcc gcctcggcat ccgctggcgc gtggcgacga tcctccgcgc cgcgaccctg
     5281 gggctcctcg tcctcgcgct cagcggtccg ctcgtccgag ttccggtccc cggggtcagc
     5341 gtcgtgttcg cggtcgaccg ctcgcggagc atcaccccgg aaggcgcgcg tgcggagacc
     5401 gccttcgtcg gggaggcgct cgcgcgcatg cggtcccagg accgggcggg ggtcgtgacc
     5461 ttcgccggcc ggccgctgtt gcgggtcccc gtggaggcgc gccccgccgt tgaggatctc
     5521 ggtcccgccc aggaccctga cgccacggat atcggcgccg cgctcgacct ggccgtccgc
     5581 atgctgcccg acgagggggt ccggcgcgtc gtcgtgctga gcgacggcgc cgagaaccgt
     5641 ggggacgctg cggcggcggc ccgcgtcgcg cgccaatccg gtgtggtcgt ggatgcggtg
     5701 cccatcgtgt ccgggacgcc gcaggaggtc ctggtcgaag aggtggccgc gccgcaggag
     5761 gtgcacccgg gcgaggtcta tgaggtccgg gcggtgctcc gggccacggg ccccgccgat
     5821 gcggtcgtga cgctctcgcg gaacggggtg gccaccgcga ccaggcatct gtccctctcg
     5881 ccgggaacga ccgcggtccc gttctcggag acggcgacgc acagcgaggt cgcgcggtac
     5941 cgcgtcgatg tcgtggcgtc tcccgcaacg attcccgaga acaagcacgg ggaagcgctg
     6001 gtcgtcgtcc gcgatcgacc ccaggtcctc ttcgtgacgg ccggcccaac ccggctgccc
     6061 caatggctcc gcgaccaggg actccggatc gatgtccaca cgcccgagga ggtgcccgag
     6121 cacctcgtcg ggttcgcgcc gtacgggagc gtggtcctaa acgatgtctc cgcgctcgac
     6181 ctgagccagg cgcagcagga aagcattcgg gcgtttgtcg agaccggggg tgggggactc
     6241 gtcgcgatcg ggggcggcca cagctatggc gtcggcggct acgccgggac gccgctggag
     6301 gacgccctgc ccgtcacgat ggacgtccgc cagacgctgg cgcttcccac cgtcgccatc
     6361 gtcctggtca tcgacgcctc ggggagcatg gaggcgttcg gcacggaact cgccaaggaa
     6421 gagctggcca aggagatcgc ctcctcggtc atcgatctgc tgggcgagca cgatcagatc
     6481 ggcgtgatca ccttcgacca ggagtaccgc tggctggtcc ccccgaccga agcgcgcgag
     6541 cgtcagcgcg tgctcgagca gatcgcccgg ctgagagccg ggggcggcac cgtgatggcc
     6601 ccgccgctcc gcgcggcggg ggagttcctc caccattcct cggccaagat ccggcacgtc
     6661 attgtcctca gcgacggcct gaccgacccg ggcgattttc gcggcatcgt cgccggaatg
     6721 gctcgggaca agatcaccgt gagcacggtc gcgatcggcc gggatgccga cctcgagttc
     6781 atgcggaacc tggcccggtg gggcgggggg cggacgtacg tcgccaagga tctgtacagc
     6841 gtgcccaaga tcttcaccgc cgaggcggtg atggccgtgc ggtcatttgt cgttgaggag
     6901 ccggtgtcgc tcacgcggag cggcgccggg ccgaccctgg cgggcctcac cccgcccccg
     6961 ccgattcgcg ggtacgtggc caccgccccg aaaccgttcg cggacattgc gctcaccggc
     7021 ccccgcgggg atccgatcct ggccagctgg cagtacggcc tcggcaggac ggtagccatc
     7081 acgtccgacg atggcgtgcg gtggaccgcg ccgtgggcgt cctggtccga cgtcgcccgg
     7141 ttctggtccc aggccgtccg ctggacgctc agcgacgacg cctcgggtct gcatctcgcg
     7201 acggcgctgg accgcagcgg gaccgggggt cgggcgatcc ttcatgcccg ccggatcgac
     7261 ggcgccccgt gggacggcct cgatgtgcgg ggggacctcg tggcccccga cggcacgcac
     7321 acggccatcg tcctggagca gaccgccccc gggcggtacg agggcgcggt cgcggccccc
     7381 ggcaccgggg tgtattccct cgccatcctt gcccgcgacg gacagcggaa cgtggggaat
     7441 cggaccgtgg gattcgtggt gccctactcg ccggagtacc gcctcccggg cgggaacctc
     7501 gcgcttctgt cccgggtggt cgagacgact ggcggcgcac tcctcgcgcg gcccgaggag
     7561 gcgttccggc cgggccgggg caccggccag cgcgagacct ggccgccgct ggccgctgcc
     7621 gcggtcgccc tgctgctggc cgaggtggcc gtccggcgtc tcccggcgct cggtcaacgg
     7681 ctcgcgatgg cgctggcggt tgccgggcac tggggcggcc gccgccaggc accctccccg
     7741 gccgtggccg cctccgacca tgcgtacgct gcggccgacc ggtgggcggt ggaggacgcc
     7801 cggtttgcgg aagaggaggc gctgcgcgcc gcctcgatgg agcaggccgc ccgcatcttc
     7861 atcacgcgcc tcagggggac gaagcggccc tagcggacga atgcaataac cgtcgggctc
     7921 agcgtgtctt tccttcaacc ttgaccgccc ggcctga
//
LOCUS       DASQGH010000580         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_19498, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000580 DASQGH010000000
VERSION     DASQGH010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19498"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..353)
                     /locus_tag="VEW91_11550"
     CDS             complement(150..353)
                     /locus_tag="VEW91_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92257.1"
                     /translation="MLRAQDLMRTSSPHPAEPPQAARSGREGPGDSAAPPRQVRQRNR
                     CSICQLQIPDQRTLCSYCRQYVS"
     gene            559..1041
                     /locus_tag="VEW91_11555"
     CDS             559..1041
                     /locus_tag="VEW91_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92258.1"
                     /translation="MQAVTLPSTATTEAPRISLRDNGWIAIPVAALVGAIRIRNFWLL
                     NYVHVFSAILWTGTDIFMGFILGPVLRRVDLPTRRAIITRLMPRMVGGPGHRGGDDRA
                     GPRFLLPINLRVYFEMCKERPYDERVRRLISRYVKIVASQAVLQFTIILIVARFVTGI
                     "
     gene            1102..2847
                     /locus_tag="VEW91_11560"
     CDS             1102..2847
                     /locus_tag="VEW91_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYM92259.1"
                     /translation="MGTTSLMDDPWNKDGDPMITPIRVLILEDTPADAELMVRELRDA
                     GFTPSWERVDTEEGFLTQLAFAPDIILADYALPRCNGLRALRLLRQLALEIPCILVSG
                     TIGEETAISCLHEGAVDYILKDRMTRLGQAVTRALEQKRLHDGKQATLAALRQSEERF
                     RGLIEHSTDGIVLIEPAGTIRYASPSITGMLGHLPEQLAERPLFNLVHPEDLPQAKAA
                     IVRVLERSGANLPIEARLRHSDGAWRWCEGTQTNLLATSGVEAIVVNFRDVTERREAD
                     ATRARLAAIVDSSDDAIISTALDGTILTWNAGAERTYGYTACEVAGRPISLLMPPARV
                     AEVSSVLERVGTGEHLRHFQTVWKHKDGTLLDVSLTLSPLLDAPGRVSGISAVARDVT
                     TLRRAEELLRIRARQQEVVAYIGRSALAGVDFDVLSAEAVSLVAQALRVGHCMVLEVH
                     AEQRAVVVRAGVRSPGATPDSWVLALAEAAFEGCAQLYDGPCIIEDVRENGHNEVPLL
                     SQLGAQSGVSVPIRSSGPAYGILGAYSVQPRPYTRTDVAFLQAVANVLAACWEYRGAP
                     EVRRKIEEIKLPSGV"
     gene            3064..>4025
                     /locus_tag="VEW91_11565"
     CDS             3064..>4025
                     /locus_tag="VEW91_11565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92260.1"
                     /translation="MTEVGPQEAPAPGGAAPPIPVDASRGAANLRSLLWLNNTLQFLS
                     NGAWYIAAPFIPLYLTSQGASVGVVGGVVGFSGVMPLLISIHAGALVDERGPAVVAKG
                     SVVLFALAGVILTILHSVWGVAAAYTLMGIGNIGFAVAPQAIVAAASNPTTRVRNYGY
                     YSLWNSAGAVVGPVIGGFVAGRFGYTAAFALVWLIMLPAFGVAASLRSVPTVPRHAVS
                     LATAHTFADTIVRQRGIGALLFISFMVVCGQTLQQSFYPLYLHKVGLSATLIGIVIAS
                     VSLSSMLVRAGLSKGVAWFGYAGSLLGATSLAAVALGITPLLRR"
BASE COUNT          665 a         1300 c         1336 g          724 t
ORIGIN      
        1 tggaccgctg ccgaacggaa ccgccggccc ttcgcgagct ctcgccacgc cacttcgtca
       61 gctgccaccg cgccgaagaa ctgcggctac ctggtgtggc gggggcaggg gcgccgagcg
      121 cgtgaaggct caaggcgaag cgccctgagc tagctcacgt actgccggca gtacgagcag
      181 agcgtccgct gatcggggat ctggagctgg cagatgctgc agcggttccg ctgccggacc
      241 tgcctcggcg gggccgcgct gtcgccgggc ccctcacgcc cgctgcgcgc ggcctgcggg
      301 ggctctgccg ggtgcgggga actggttcgc atgagatcct gagcacgaag cacctggaac
      361 acctcccaga ccataagcgc tgaccgggcc ccccgccggt tcccggtttc gggcgtcggc
      421 caaggcccgg ggggatgtcc caggcgcggc gccggccggg gctcctggag ggcgctcccg
      481 gcgaccaacc ccgcagggaa accggctccg gacgctgtcg aagggagcaa cgcggaaacc
      541 tgtgggaacc cgagccacgt gcaggctgtg acgctccctt cgaccgccac gaccgaggcg
      601 ccccgcatct cattgcggga caacgggtgg atcgcgatcc ccgttgccgc gcttgtggga
      661 gcgatccgca tccgcaactt ctggcttctc aattacgtcc acgtcttcag cgcgatcctg
      721 tggaccggga ccgacatctt catggggttc atcctcgggc cggtcctcag gcgtgtcgac
      781 ctgccgaccc ggcgggccat catcacgcgc ctcatgccgc gtatggtggg cggccctggc
      841 catcgtggcg gtgatgaccg tgcagggcct cggtttctgc tccccatcaa cctccgggtg
      901 tactttgaga tgtgcaaaga gcggccgtac gatgaaaggg ttcgccggct catcagccgc
      961 tacgtgaaga tcgtggcaag ccaggcggtg ctgcaattca cgatcatcct gatcgtggcc
     1021 aggttcgtca ccgggatcta ggctcaccgg gttcgcaagt tcccgcgaag atggcggctg
     1081 gaagtctaga agtttgccga aatgggcacc accagcctaa tggatgaccc atggaataag
     1141 gatggcgatc ccatgatcac tccgattcgc gtcctgatcc tcgaggacac accagctgac
     1201 gccgaactca tggtccgtga gctgcgcgat gcgggattca caccgagctg ggagcgcgta
     1261 gataccgaag agggcttcct gacccagctg gccttcgccc ctgatatcat cctggccgac
     1321 tacgcgctcc cacgatgcaa cggactgcgt gcgctgcgtc tcttgcgcca actcgccctc
     1381 gagatcccgt gcatcctcgt ctcggggacc ataggagaag aaacggctat ctcctgtctg
     1441 cacgaaggcg cagtcgatta cattctcaag gaccggatga cgcggctcgg tcaagcggtc
     1501 acgcgggctt tggagcagaa acggctgcac gacgggaagc aggcgacgct ggccgcgctg
     1561 cgccagagcg aagagcggtt tcgtggcctt attgaacaca gcaccgacgg catcgtgctg
     1621 atcgagcctg ccggaacaat ccgttacgcg tccccctcca taacaggaat gctgggccat
     1681 cttccggagc agctagccga gcgtccgttg ttcaatctcg tccaccccga agatctcccg
     1741 caggcaaagg cggccattgt tcgggtgttg gagcgatcgg gggcgaacct cccaatcgag
     1801 gcgcgcctgc gtcatagcga cggcgcctgg agatggtgcg aagggacgca gacaaacctg
     1861 ctggccacgt ccggtgtgga ggccatcgtg gtcaacttcc gcgacgtcac cgaacgtcgg
     1921 gaagcggacg cgacgcgtgc caggctcgcg gccattgtgg attcatcgga tgacgcgatc
     1981 atcagcaccg ccctggacgg aacgattctg acgtggaatg ccggtgcgga acgtacgtat
     2041 ggctacacgg cgtgcgaagt ggcgggacgc ccgatatcgc tcctcatgcc cccggcccgg
     2101 gtggccgaag tctcgagcgt cctcgagcgg gttgggacgg gagaacatct tcggcacttc
     2161 caaacggttt ggaagcacaa ggacgggacc ctactcgatg tctcgctcac gctgtcgccc
     2221 ctgctggatg cgccggggcg agtatcgggc atctccgccg tcgcacgcga cgtcacgacc
     2281 ttgcggcggg ccgaggagtt gctgcgcatt cgcgcccgcc aacaggaggt tgtggcctac
     2341 attggccgct cggcgctggc aggggtcgac ttcgatgtgc tgagcgccga agccgtttcg
     2401 ctcgtcgcgc aggccctccg cgtggggcat tgcatggtac ttgaagtcca tgccgagcag
     2461 cgcgcggtgg tcgtgcgggc gggtgtgcgg tcgccgggcg ccacccccga ctcgtgggtg
     2521 ctggcgctgg cagaagccgc cttcgagggg tgcgcgcaac tttacgacgg gccgtgcatc
     2581 atcgaggacg tacgagaaaa tggtcacaac gaggtgccgt tgctatctca gctgggcgcc
     2641 caaagcggcg tgagcgttcc catccgcagc tccgggccag cctacggaat cctcggggcc
     2701 tacagcgtcc agccgaggcc ctacacgaga accgacgtgg cgtttctcca ggccgtcgcg
     2761 aacgtgctcg cggcctgctg ggagtatcgc ggcgcaccag aggttcgcag gaagatagaa
     2821 gagatcaaac tccccagcgg cgtgtagggg ttccacccgt caatcaatat gtgggacgta
     2881 atggtaaagc acatctatac gatttccgat gcgctggccg atgggatcgc caagcaattc
     2941 ccggacaagt ttcagtaaca ggagtgacgc acgacgctga aaggtagggg ccgggtcagg
     3001 aggtgctccc tgcgtgccgc agaagccgta agatactcga gacgccggct tccaggagcg
     3061 ttgatgaccg aagtagggcc tcaagaggcg ccggctcccg gcggggcggc tccgcccatc
     3121 ccagttgacg ccagccgggg tgccgcgaac ctgcgctcgc tcctctggct caacaacacc
     3181 ctgcagttcc tctccaacgg tgcgtggtac atcgccgcgc cgttcatccc cctctaccta
     3241 acctcccaag gtgcgtcggt gggcgtcgtg gggggcgtcg tcggtttttc cggcgtcatg
     3301 ccgttgctga tctcgatcca tgccggtgcg ctggtcgatg agcgcggccc ggcggtggtc
     3361 gcgaaaggtt cggtggtcct gttcgctctg gccggtgtga tcctcacgat cctgcacagc
     3421 gtgtgggggg tggccgctgc atacaccctg atggggatcg ggaacatcgg cttcgctgtg
     3481 gcaccacagg ctatcgtcgc cgcggcaagc aatcctacca cccgcgtccg caactacggc
     3541 tactattccc tgtggaactc cgccggggcg gtggtcggtc ccgtgatcgg cgggtttgtc
     3601 gccggccgct tcggctacac ggcggcgttc gcgctggtct ggctcatcat gctccccgca
     3661 ttcggggtcg ccgcctccct acgcagcgtg ccgacggtcc cgcggcacgc cgtgtcgctc
     3721 gcgaccgccc acacgttcgc tgacacgatc gtgcgccagc gggggattgg ggccctccta
     3781 ttcatctcgt tcatggtggt gtgcgggcag acgctgcagc agtccttcta tccgctctac
     3841 ctgcacaagg tcgggctgtc ggcaaccctg atcgggatcg ttatcgcctc ggttagtttg
     3901 agctcgatgt tggtccgcgc gggcctgtcg aaaggcgtgg cgtggtttgg ctacgcggga
     3961 tcactgctcg gcgcgacatc gctcgcggcg gtcgcgctcg ggatcacacc gctgcttcgg
     4021 cggtt
//
LOCUS       DASQGH010000581         1993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5270, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000581 DASQGH010000000
VERSION     DASQGH010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5270"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VEW91_11570"
     CDS             complement(<1..644)
                     /locus_tag="VEW91_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYM92261.1"
                     /translation="MPSFRASVDRHLEAFLTDLRTFVEVESPTVDKAACDRAGRHLAA
                     RFREYAGAEIVWHPQAEWGDHFEARIGNGRRRILLLGHFDTVWALGTLGRLPCRITGD
                     RMTGPGCFDMKYGDMQALWGLRFVVESGAAKDKAFVFFGNSEEEVGSPSSRPIIERLA
                     RESECVLVLEPSVGAEGAVKLWRKGVGMYHLDVDGVASHAGADPEKGRSAILELA"
     gene            complement(664..1710)
                     /locus_tag="VEW91_11575"
     CDS             complement(664..1710)
                     /locus_tag="VEW91_11575"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYM92262.1"
                     /translation="MHFTVVGAGAIGGTIGAHLARAGHAVLLVDAAEDHVLAIERAGL
                     TIEGRTTFRIRVPAVTVAGLADALRGRPPDAVLLAVKAMHTAVALEPVVPLLGPESFV
                     VSMQNGLNEKVIAGRIGAARTVGAFVNFGADYLEPGRIVYGGAGALYLGELDGRTTPR
                     LEGLGQVLRESFLPHTTLTSNIWGYLWGKLGYASMLFATATVDAPMADVLDDRASRPL
                     LANLAGEVVRVADAEGVRSEGFDGYDPNAMRFAAPRDWAAIHQSLDRLAEFNRRSLKQ
                     KSGIWRDLVVRRRRTEVDDQIGPVVEAAGAHGLDVPLNRHLSEIIHALEEGRRPMARA
                     NLDELCRLNEELYH"
     gene            complement(1803..>1993)
                     /locus_tag="VEW91_11580"
     CDS             complement(1803..>1993)
                     /locus_tag="VEW91_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HYM92263.1"
                     /translation="ERVRELAEQFRDREVPRPPHWGGYRLIPEAIEFWQSRPSRLHDR
                     LRYRLLGGREWRIERLSP"
BASE COUNT          284 a          760 c          663 g          286 t
ORIGIN      
        1 gccagctcga ggatggcgct gcgccccttt tccggatcgg ctcccgcgtg gctcgccacc
       61 ccgtccacgt cgaggtggta catgccgacc cccttccgcc acaatttgac cgcgccctcg
      121 gcgcccacgg acggctcgag cacgaggacg cactccgact cgcgggccag tcgctcgatg
      181 atggggcggg aggaggggct gcccacctcc tcctccgagt tgccgaaaaa cacgaacgcc
      241 ttgtccttgg ccgcgcccga ctccaccacg aaccgaagcc cccacagcgc ctgcatgtcg
      301 ccgtacttca tgtcgaagca gcccggcccg gtcatccggt ctccggtgat gcggcagggg
      361 aggcgcccca gcgtccccag cgcccagacc gtatcgaagt ggccgagcag caggatccgg
      421 cggcggccgt tcccgatcct cgcctcgaag tgatcgcccc actccgcctg ggggtgccag
      481 acgatctcgg cgccggcgta ttcgcgaaac cgcgcggcga ggtgccggcc ggcgcggtca
      541 cacgccgcct tgtccaccgt gggtgattcg acctcgacga aggtccggag gtcggtgagg
      601 aacgcttcga ggtgccggtc aaccgacgcg cggaaactgg gcacgggttc ctccctggaa
      661 cgctcagtga tacagctctt cgttgaggcg gcatagctcg tcgaggttgg cgcgggccat
      721 cgggcgccgc ccctcctcga gcgcgtggat gatctccgac aggtgccggt tgagcgggac
      781 gtcgaggccg tgggcgcccg ccgcttcgac gaccgggccg atctggtcgt ccacctcggt
      841 gcggcggcgc ctgacgacca ggtcgcgcca gatcccgctc ttctgcttca gcgaccggcg
      901 gttgaactcg gcgagccggt cgaggctctg gtggatcgcg gcccaatcgc ggggcgcggc
      961 aaagcgcatc gcgttgggat cgtacccgtc aaacccctcg ctgcgcaccc cctccgcgtc
     1021 ggcgacgcgg acgacctccc cggccagatt ggccagcaag gggcgggacg cccgatcatc
     1081 gagcacgtcg gccatcggcg catcgaccgt ggccgtcgcg aacagcatcg aggcgtaccc
     1141 cagcttgccc cagaggtatc cccagatgtt gctcgtgagc gtggtgtgcg ggagaaacga
     1201 ctcccggagg acctgtccca gcccctccag gcgcggggtc gtgcggccgt cgagctcgcc
     1261 gaggtacagg gcccccgcgc ccccgtacac gatgcgcccg ggctccaggt agtctgcccc
     1321 gaagttgacg aacgcgccca cggtccgggc cgccccgatc cgtccggcga tgaccttttc
     1381 gttgagcccg ttctgcatcg agaccacgaa actctccggg ccgagcagcg ggaccacggg
     1441 ctcgagcgcc accgcggtgt gcatcgcttt gaccgccagc agcaccgcgt ccggcgggcg
     1501 gccgcggagg gcgtcggcca gccccgcgac cgtcaccgcc ggaacgcgga tccggaaggt
     1561 cgtgcggcct tcgatggtca gacctgcgcg ctcgatcgcc aggacatggt cctccgcggc
     1621 gtcgaccagc agcaccgcgt gcccggcccg ggccagatgc gcgccgatgg tgcccccgat
     1681 cgcgcccgcc ccgaccaccg tgaagtgcac cgctctcctc cccctctacg cggcggccgt
     1741 gctgcggagg caccctccgc agactagtac gccgccgcct caccgtttgc ctgcgcggac
     1801 gcctacggcg acagccgctc gatccgccac tcccgccccc ccagcaggcg gtagcgcagg
     1861 cggtcgtgca gccggctcgg gcggctctgc cagaactcga tcgcctccgg gatgagccgg
     1921 taccctcccc agtgcggcgg ccgggggacc tcgcggtccc ggaactgctc cgccagttcc
     1981 cgcacacgct cgt
//
LOCUS       DASQGH010000582         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_25351, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000582 DASQGH010000000
VERSION     DASQGH010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25351"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VEW91_11585"
     CDS             complement(<1..372)
                     /locus_tag="VEW91_11585"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYM92264.1"
                     /translation="MKWVTRKNANVDRIACPWLIRRFVDTTPEFIYVPAERVLEVAER
                     EQAIPYDVKGVELGHVGGRCSFESIMVKYNLTDPALQKLATIVHGADVAADIRIVPEA
                     AGLRAIAQGFALVHGDNDHEKI"
     gene            453..1418
                     /locus_tag="VEW91_11590"
     CDS             453..1418
                     /locus_tag="VEW91_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYM92265.1"
                     /translation="MPWVVFSHSLPSAARSSPRVAVWRRLRALGAISPKGGVHVLPAR
                     DECVEAFQRLAQEVEQARGEALLMRVERFEGLSDAQLIGLFQEARKEDYAALDGRAAV
                     LEKALAGPRTRGPRDDAQARESLARLRREHAAIARIDFFDSPAGVQVASRLARIEESL
                     SPAQPDHTPVAPAALSAYRTKRWVTRPSPHVDRLGCAWLIRRFINPRAVIRYSTAPRP
                     DEVAFDMDRGPFSHHGPLCTFETMVRAFGLDDPAVGVIAEIVHEIDLRDGRYVRPEVP
                     GIDAILRGWMSRTDADREAHGVALFEGLYAGLSPAPSRRRTKKAR"
     gene            1540..2043
                     /locus_tag="VEW91_11595"
                     /pseudo
     CDS             1540..2043
                     /locus_tag="VEW91_11595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2175..2714
                     /locus_tag="VEW91_11600"
     CDS             2175..2714
                     /locus_tag="VEW91_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92266.1"
                     /translation="MAGGFVVQSLVAYWFHQRFGVSLEQLGPLFFGTNLLSALSALAA
                     AALADRFGLLNTMVFTHLPSNVLLALVPFMPSWPVAAGVLLARHALSQMDVPTRQAYT
                     MVLVAPDKRAAAAGLTNAVRPAASSLAPAISGAALQTAANGLPFVLAGGLKILYDITL
                     WLMFRRASLGEHRHDGTSS"
     gene            3185..4129
                     /gene="rtcA"
                     /locus_tag="VEW91_11605"
                     /pseudo
     CDS             3185..4129
                     /gene="rtcA"
                     /locus_tag="VEW91_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013317.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
BASE COUNT          585 a         1445 c         1385 g          714 t
ORIGIN      
        1 gatcttctcg tggtcgttgt ccccgtggac cagggcaaag ccctgggcga tcgccctgag
       61 gcctgccgcc tcggggacga tcctgatatc cgcggccaca tccgccccgt gaacgatcgt
      121 ggccagtttc tggagcgccg gatcggtgag gttgtacttg accatgatcg attcgaagct
      181 gcaccggccg ccgacatggc ccaactcgac gcccttgacg tcgtagggaa tcgcctgttc
      241 ccgctcggcc acctcgagga cccgctccgc cgggacgtag atgaactcgg gggtcgtgtc
      301 gacgaatctc cggatgagcc aggggcaggc gatccggtcg acgtttgcgt tcttgcgcgt
      361 cacccacttc atggccgaca cctccggcgg ctgggaaaat gtaataagta ttgcatacat
      421 tagcaacata tgatactgtc gtcaagacgc cgatgccctg ggtcgtcttc tcccactcgc
      481 tgccgtccgc ggcgcggtcg agcccgcggg tcgcggtgtg gcgccgcctg cgcgcgctgg
      541 gcgccatctc gcccaaaggg ggcgtgcacg tcctccccgc gcgggacgag tgcgtggagg
      601 ccttccaacg gctcgcccag gaggtcgagc aggccagggg agaggcgctg ctcatgcgcg
      661 tcgagcggtt cgaggggctg agcgatgccc agctcatcgg gctctttcag gaggcgcgga
      721 aggaagacta cgcggcgctc gatgggcgcg ccgcggtcct tgagaaagcc ctggccggcc
      781 cgcgaacacg cggtccccgg gacgacgcgc aggcgcggga gtcgctggcc aggctcagga
      841 gagagcacgc ggcgatcgcg cggatcgact tcttcgactc tcccgcgggg gtgcaggtcg
      901 cctcgcggct cgccaggatc gaggagtccc tctccccggc ccagccggat cacacgccgg
      961 tggccccggc cgcgctctcc gcctaccgga ccaaacgctg ggtgacgcgg cccagcccgc
     1021 acgtcgaccg tctggggtgc gcctggctga tccggcgctt catcaatccg cgcgccgtga
     1081 tccgctactc gaccgccccc cggccggacg aggtcgcctt cgacatggac cggggcccgt
     1141 tcagccatca cgggcccctg tgcacgttcg aaacgatggt cagggcgttc gggctggacg
     1201 accccgcggt cggggtgatc gcggagatcg tccacgaaat tgacctgcgc gatgggcggt
     1261 acgtccgtcc cgaggtgccc ggcatcgacg ccatcctccg gggctggatg tcgcggacgg
     1321 acgccgaccg cgaagcccac ggggtcgccc tcttcgaggg gctctatgca gggctatccc
     1381 cggcgccctc tcggaggcgc accaagaaag cgaggtgacc cggcacaggg gaagcgaagc
     1441 tgatcgcgaa gtcgacgggc ttcctcgccg aatgcgatac cgcatcagcg ggctctgacg
     1501 cgggacggat atttgcttct cgcagcgcgg gggctgcgga tgcactccta cggcttcctg
     1561 tctgtcatcc tcgccctgta tctatccggc ctgggcctag cgatggtgca aatcggcgtg
     1621 ctgttcacag tggcgctcgc cggcggagcc gccgtcacca ctggcgtgtc gatgttcgcg
     1681 gatcgctggg gacggcggcc gaccctgatc gcctcggccg tcctcatggc gctcggcggg
     1741 gtcgcgctga ccaccggcga gttccccctc ctgctcgtct tcgcggccct cgccaccctg
     1801 agcccgagcg cgcaggagat cgggccattc cagtccctgg agcaggcggc gctgtcggag
     1861 gcgggcgccg agccggggcg cgtgatgccg tacgcctggt acaatctcgt cgggtacgtg
     1921 gcggttgccg tcggcgccct ggtagccggg gcggcccccg ccgcgcttca agccgccggg
     1981 tggtcggcgc tcgaggccca gagggcgatg gtctgggcgt tcgctgcgac cggccttgcg
     2041 ctcgcagggc tgtacaccct cctctcgtcg gcggtagaag cctcgccacg accccactcg
     2101 gcgcgtcgat cttatggcct gcaccggtcc cgcggcatcg tgatccgccg agcgcattgt
     2161 tcggcgtcga tgcgttggcc ggggggttcg tcgtacagag cctggtggcg tactggttcc
     2221 accagcggtt cggggtcagc ctggagcagc tcggcccgct cttcttcggg accaacctct
     2281 tgtcggcgct ctcggctctg gcggccgccg cgcttgcgga ccggttcggg ctgctcaaca
     2341 ccatggtgtt cacgcatctc ccctcaaacg tgctcctggc cctcgtgccg ttcatgccgt
     2401 cctggccggt cgccgcgggg gtgctgctcg cgcgacatgc gctgtcgcag atggacgtcc
     2461 ccacccgcca ggcctacacg atggtcctgg tcgcccccga caagcgggcc gccgccgcgg
     2521 ggctgaccaa tgcggttcga ccggccgcat ccagccttgc gccggcgatc tccggggccg
     2581 cccttcagac ggcagccaac ggcctcccct tcgtcctagc cggcgggctc aagatcctgt
     2641 acgacatcac cctctggctg atgttccggc gcgcgtcgct cggcgagcat cgacacgacg
     2701 gcacgtcgtc gtaaccgcaa ccgccgggcc gcagacaggc cgaacccttc actctgcaac
     2761 agctcccgca cgcggatcac cgcctgctcg tccggcaggc tgacccgcgt ttccaatcca
     2821 gaccatgtga aggggggacg tcaacccctc ccgccgggag cccgaaccgt tgacccgccc
     2881 gggcggcccg acgtcgtgcc atcaggtctc ggacctattt gtcgctcggg ccgccgtccg
     2941 gtcgtccgtg gcgaggccat agccgccgaa acaacgccat gatctccgct gcacgcaccg
     3001 tccgggttcc ccatctcctc ggccagagaa gcggagcgcc gttcgcgagg gcctcgcgac
     3061 ctgggcacga aatcccgtac tgaaccgatg gccgcctgga cgattttccc tcataatcgt
     3121 gctgaccatg cgtgcaggag gacccgcgcc gctcggtacc tgaccgcggc aatatgagtc
     3181 ttggatgatt ttgctcgacg gttcacaaaa atcagggagc ggcaccgtcg tgcggtacgc
     3241 cgttgcgctg gccgcacttc tgggtcgcga attgcgactg acaaatgcca gggcaaggcg
     3301 aaggccgccc ggcttgcggc cgcaacacct ccgcaccgtc gaggcggttg ctcagattgc
     3361 cgaagggcgc ctcaacgggg ccgctgtagg cgcgaccaag ttcaccttcc gtccctccgg
     3421 cggcgttcgg ggaggtcggt atgtttggga gatcggcacc gccggctccg ccgctatgtt
     3481 ggcgttcacc tgctgcccgt ggccgcgttc gccgatgccc cctgccattt tgagatccac
     3541 ggcggcctct ttcaggactt tgctccctcg gcgtttcact tccaacacgt cctgctgcca
     3601 ttattggccc ggatgggcct tcgcgcggac ctgcgcatct tgaggcccgg attcgtcccc
     3661 aagggaggtg gcatcatttt gcttgcggtg caccccgtcg ccaatgcctt gcgcccgctg
     3721 cagctcttgg atcaaggccg ggtgacgcgg gtctggggga ccgccctctc gccgcatttg
     3781 cgccgccagc gggtcagcga tcggatggcg cggcggtgtc gcgaccggct cgcggaaagc
     3841 ggcctggatg ccacgattga cgcctgttac gatgagaccg ccctccagcc cggggcggcc
     3901 ttggcggtgt tcgccgaatc ggataccggc tgtatcctgg gcgctgaccg ggcgggcgcc
     3961 cccgggcggc cgtcggaagc catagcggat gacgtggcgc ggatgctgct ggaggatctc
     4021 aggtcggggg cgaccgtcga ccgccatcta gcagaccaac tcgtcctgtt cgcggcgctg
     4081 gccgacggca aaagcgagta tatcatcccg agcgtgacag aacacgcgg
//
LOCUS       DASQGH010000583         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10903, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000583 DASQGH010000000
VERSION     DASQGH010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10903"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VEW91_11610"
     CDS             complement(<1..298)
                     /locus_tag="VEW91_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92267.1"
                     /translation="MGDFAVSGGKIPRRNGCTMKSWERIIGKDELEVYRRAGYGRLPG
                     ERRRPAVLVVDMEYNFTGEKPEPILKSIAKFRNSCGEAAWASIPKIARLLRVARS"
     gene            523..942
                     /locus_tag="VEW91_11615"
     CDS             523..942
                     /locus_tag="VEW91_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HYM92268.1"
                     /translation="MRRLAIEIDGVKAGAELWDERAPKTVAALLRHLPIDDRTIHVRW
                     SGAAWRTEKNYPLGVGDIENRATWLERGDIIYYDDPRYELYKVAFIYGTSQWRDDKGE
                     LYVARIGRVTENVDAFIKASEKVLFEGPKRIAIRVMP"
     gene            1065..>2098
                     /locus_tag="VEW91_11620"
     CDS             1065..>2098
                     /locus_tag="VEW91_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92269.1"
                     /translation="MTRRGLLRTTVAAGAALALDKYAGAIPAAEGAPSPQRGGTLRVG
                     WIPNAHTLDPHYSVDFAERHVMYAVYNTLVAVSPTFEIMPELARSWKIGPDGKAITFE
                     LQPNVKFHDGTPCDAAAVKWNMDFIVDAQNTSPQRRQLEPFLAGVDVAGPTTVIFRLK
                     KPYPGLLAAMAERPGFIVSPSAWQKSGRDLGRHPVGTGMFEFVEWMSDDHVMLKRFDG
                     YWDRGMPYLDRINYRVIPDPTVRGTMVRTGEIDTSSEPDPKDVPVLERTAGVKVVTQN
                     PSGHWWATQWRVDRPPFNNKALRQAIAYGVDREEFVKVMLGGRGKVAAGPTPSNVWWY
                     NASAKGYTYDP"
BASE COUNT          395 a          628 c          694 g          381 t
ORIGIN      
        1 gggagcgcgc cacgcggagg aggcgggcaa tcttcgggat gcttgcccag gccgcttcgc
       61 cgcagctgtt gcggaatttg gcgatcgact tcaggatcgg ttctggcttc tctcccgtaa
      121 agttgtactc catatcgacg accagcaccg cgggtcgccg cctttcgccc gggagccgcc
      181 cgtagcctgc ccggcgatag acttccagtt cgtctttccc gatgatgcgt tcccaggatt
      241 tcatcgtgca accatttcgg cgtgggatct tgccgccgga caccgcgaaa tcgcccatcg
      301 gggtccggta tcactggtct ggtcctatgc ataccacggg attttgaggt atgtcaattc
      361 gcgtgtccgt tccgtgggca ggactcggcg ccctggtttg gaaatagaat cggcggcgga
      421 ccccgcgaag ggcaggctcc tgagatcgca aagcatgatc atgcgaggtc cccattgtcc
      481 ccgcagtccc tttgtgatgc ggtagggtgg gaggcgagaa gcatgcgacg gctggccatt
      541 gaaatcgacg gcgtcaaagc cggggcggag ctctgggacg agcgcgctcc gaagaccgtt
      601 gccgcacttc ttcgtcatct gcccatcgat gacaggacga ttcatgtccg gtggtccggt
      661 gcagcctggc ggaccgagaa gaactatccc ctcggggtgg gggatattga aaaccgggcg
      721 acctggttgg agcgcggcga catcatctac tatgatgatc cacgctacga gctctacaag
      781 gtcgccttca tctacggcac gtctcagtgg agggacgaca agggcgagct gtatgtggca
      841 cgaatcggcc gtgtgacgga gaatgtcgac gcctttatca aagcgagcga gaaagttctc
      901 ttcgagggtc cgaagcgaat cgccattcgc gtaatgcctt aggatgcgtg tgcgaagatc
      961 cgcacgcggt tccaagtagc gtacaggggg ggcgtgatat gacgggaggt acgcacaagc
     1021 cggattcctc gagcagaaag gacgcctgct ccacggggga aaagatgacg cggcggggcc
     1081 tgctccgcac gacggtcgct gccggcgccg cgcttgcact cgacaaatac gctggtgcga
     1141 ttcccgcggc ggagggtgcg ccgtcgcccc agcggggggg aacgcttcgg gtgggatgga
     1201 tccccaacgc ccacacgctg gatccacact actccgtcga ctttgccgaa cgacatgtga
     1261 tgtacgccgt ctacaacacc ctggttgcgg tcagcccgac gtttgagatc atgcccgagc
     1321 tggcgcgctc atggaagatc gggccggacg gcaaggccat taccttcgag ctgcagccga
     1381 acgtgaagtt ccacgatggc accccgtgcg atgccgccgc ggtcaagtgg aacatggact
     1441 tcatcgtgga cgcccaaaac acttctcccc agcgcaggca actggagccg tttctcgcgg
     1501 gggtcgatgt cgccgggccg acgacggtca tatttcgctt gaagaaaccg tacccggggc
     1561 tgctggccgc gatggcggag aggcccggat tcatcgtgtc gccgtcggcc tggcagaaat
     1621 ccggcaggga tctcgggcgc catcccgtgg gcacgggcat gttcgagttc gtcgagtgga
     1681 tgtcggatga ccacgtgatg ctcaagcggt ttgacggcta ctgggaccgg gggatgccgt
     1741 acctcgatag gatcaactat cgcgtgatcc cggacccgac ggtgcgcggc accatggtgc
     1801 gcacgggaga gatcgacacc tcgagcgaac ccgatcccaa ggacgtcccg gtgctcgaac
     1861 ggaccgcggg ggtgaaggtc gtcacccaaa atccctcggg ccattggtgg gccacccagt
     1921 ggcgggtgga ccgtcccccg ttcaacaaca aggcgctgcg gcaggcgatt gcctacggcg
     1981 tcgaccggga agagtttgtg aaggtgatgc tgggcggccg cggcaaggtc gccgcgggcc
     2041 ccacgccctc caacgtctgg tggtacaacg ccagcgccaa agggtacacc tacgaccc
//
LOCUS       DASQGH010000584         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2464, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000584 DASQGH010000000
VERSION     DASQGH010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VEW91_11625"
     CDS             complement(<1..358)
                     /locus_tag="VEW91_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92270.1"
                     /translation="MAGRRVGRRPVGRLLVLIALAGLAVVYFFPFAWMLSTSLKPTQE
                     LYTQPPTLWPVHPTLEAYQAALVGARKWVLLRNSLGVCLATVVLALALALMIAYPLTR
                     LPVSPGFRRGVFSWLLS"
     gene            complement(358..1263)
                     /locus_tag="VEW91_11630"
     CDS             complement(358..1263)
                     /locus_tag="VEW91_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYM92271.1"
                     /translation="MGAASFPVHPTAAARRRRVAPYLLVAGPILFLLAVVVYPMGYAI
                     GLSLQYYRVGGFAGLHNFAFAFQDRLLGDAVRATLVYVVIAVGTEVVCGLALAVAVHR
                     TIRSALGRTLAYLLFIVPMVTPPVAAGVSFRLMYIPEYGILNVLLHALGYHGRPILWM
                     SSPHMAMFSVISVDVWQWMPFAFLVLFAGLQAVPQDVVEAAEVDGAGGWARFWEVDLP
                     YLRSLLLLVLIFRVTDALRVFDHVQVLTMGGPGAATEFLSLYLYKIAFKFSNLNYASA
                     LALYIVVVISIAFALFSRYLTEETA"
     gene            complement(1271..>2112)
                     /locus_tag="VEW91_11635"
     CDS             complement(1271..>2112)
                     /locus_tag="VEW91_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM92272.1"
                     /translation="GNVVMFMHRRALIPQPPQTWDEVLANGTHLSNPASQFYGFAMRG
                     AKGNPIISQWYPILAAFGGRVFDDNWNVVFQSDASVNALKFFIGQLKAIAQPGPDTAD
                     AADRTRLVATGHAAQASVWPAEASDIVENPQVSKVIGKIAYTVMPAGPSGRHTPMMGN
                     WLLAIPKAAKEKAWAFRFITWATSKEIQRPYAEAGGIPFRNSILTDPALNQRYPFFRA
                     MADSLAAPPFWRPRTTEWSAVESIHGTHVNAALVGTESPEQAVARAASEITQHMKEAG
                     YIK"
BASE COUNT          368 a          714 c          749 g          281 t
ORIGIN      
        1 ggctcaggag ccacgagaac acgccccggc ggaacccggg cgacaccggg aggcgggtga
       61 ggggataggc gatcatgagc gcgagggcca gcgccagcac caccgtcgcc aggcacacgc
      121 ccaggctgtt gcggaggagc acccatttcc gggcccccac cagcgccgcc tggtacgcct
      181 cgagggtggg gtggaccggc cacagggtcg gcggctgcgt gtacagctcc tgggtcggct
      241 tcagcgaggt cgagagcatc cacgcgaacg gaaagaagta gacgacggcc aagcccgcga
      301 gcgcgatcag gacgaggagg cggcccaccg ggcggcgacc cacgcggcgg ccggccatca
      361 ggccgtctcc tccgtgagat accggctgaa cagggcaaac gcgatcgaga tcacgacgac
      421 gatatacagc gccagcgccg aggcgtagtt caggttgctg aacttgaacg cgatcttata
      481 gaggtacagg ctcaagaact ctgtcgcggc cccgggcccg cccatcgtca gcacctgcac
      541 gtggtcgaag acccgcagcg cgtccgtcac ccggaagatc aggacgagga ggaggagcga
      601 cctcaggtac ggcaggtcga cctcccagaa ccgcgcccac cccccggccc cgtccacctc
      661 cgcggcctcg acgacgtcct gcggcacggc ctgaagcccg gcgaacagca ccaggaacgc
      721 gaacggcatc cactgccaga cgtccacgct gatcaccgag aacatcgcca tgtgcgggct
      781 ggacatccac aggatcggcc gcccgtgata cccgagcgcg tgcaggagca cgttgaggat
      841 gccgtactcc gggatgtaca tcagccggaa gctgaccccg gcggccacgg gcggtgtgac
      901 catgggcacg atgaacagca ggtaggcgag cgtccggccg agggcgctcc ggatggtgcg
      961 gtgtaccgcg accgcgaggg cgaggccgca gacgacctcg gtgccgacgg cgatgacgac
     1021 gtacaccagc gtggcccgca ccgcgtcccc gaggagccgg tcctgaaacg cgaaggcgaa
     1081 gttgtggagc cccgcaaacc cgccgacccg gtagtactgc aggctcaggc cgatcgcgta
     1141 ccccatcggg tacaccacga ccgcgagcag gaacaggatc ggccccgcga ccagcaggta
     1201 gggcgcgacc cgccgccggc gcgccgccgc tgtggggtgg accgggaagg acgccgcccc
     1261 caccgccggc ctacttaatg tagcccgcct ccttcatgtg ctgggtgatc tccgacgccg
     1321 cccgcgccac ggcctgctcg ggagactcgg tcccgacgag cgcggcgttg acgtgggtgc
     1381 cgtggatcga ctcgacggcc gaccactcgg tcgtgcgggg ccgccagaac gggggcgccg
     1441 cgagcgagtc ggccatcgcc cggaagaatg ggtagcgctg attgagcgcg ggatcggtga
     1501 ggatggagtt gcggaacggg atgcccccgg cctccgcgta cggccgctgg atctccttgc
     1561 tggtcgccca cgtgatgaac cggaaggccc aggccttttc cttcgcggct ttggggatcg
     1621 cgaggagcca gttgcccatc atcggcgtgt gtctgccgga ggggccggcc ggcatcaccg
     1681 tgtatgcgat cttgccgatc accttgctga cctgggggtt ctcgacgatg tcggacgcct
     1741 cggcgggcca cacgctcgcc tgggcggcgt gcccggtggc caccagccgg gtccggtcgg
     1801 ccgcgtcggc ggtgtccggg ccgggctggg cgatcgcctt gagctgcccg atgaagaact
     1861 tgagcgcgtt cacggacgca tcggactgga acaccacgtt ccagttgtcg tcgaacaccc
     1921 gcccaccgaa cgcggcgagg atcgggtacc attgggagat gatcgggttg cccttggcgc
     1981 cccgcatcgc gaacccgtaa aactggctcg ccgggttgct gaggtgcgtg ccgttcgcga
     2041 gcacctcgtc ccacgtctgg gggggctgcg ggatcagggc gcgccggtgc atgaacatga
     2101 cgacgttgcc ga
//
LOCUS       DASQGH010000585         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42179, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000585 DASQGH010000000
VERSION     DASQGH010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42179"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VEW91_11640"
     CDS             <1..435
                     /locus_tag="VEW91_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYM92273.1"
                     /translation="FFVLFSFGWARPVPVNPQNFANPRRGMLTVALAGPLANIVVAFA
                     IGGLVKAPGLIGTPWGDLASMLVWINVVLAVFNLIPIPPLDGSRILEGLLPSEQAIAY
                     ARIQPYGTVLILVLLYTGVVGQVMFPAVRWLYGASTGTGFHL"
     gene            555..1274
                     /locus_tag="VEW91_11645"
     CDS             555..1274
                     /locus_tag="VEW91_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HYM92274.1"
                     /translation="MGYHVQSDTFTGPLDLLVSLARRGQVDLRALSLRSIADGYVAQA
                     RGALDLDEATEVLVDLAVLADLKVRTLVPNAPPAEEVLEEGEGASDLRDRLGAQMAGY
                     LKFREAALALRVLEEVQRQVFVRAPNAPDPTGEVLVDGVTLQDLFAAFAQVLRRAREA
                     PQEIAGEEFTVEQKMDALVALLEEAPGGVAFGALFREGASRLEIIVTFLAMLELIRRR
                     RIRVRQVQVFGEIVVALAVPV"
     gene            1285..1953
                     /gene="scpB"
                     /locus_tag="VEW91_11650"
     CDS             1285..1953
                     /gene="scpB"
                     /locus_tag="VEW91_11650"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYM92275.1"
                     /translation="MSDPLGPVKASEAAPALIQQDWPEGNGHAVAVTPEGPAAADRAE
                     GGLAIRNAVECLLLARGGPVRLSDLRKVLDVEEAELRATMAALQVEYEGRGLQIQEIA
                     EGYQLCTRPEYAAVVQRLLRLNELEPLTRASLEVLAIVVYRQPVTRAEVDAIRGVQSV
                     HHLVKLQDRRLIQELGRRPGPGRPILYGTTEGFLRYFGLKDLGALPRIGDHDLLPALV
                     PPAR"
     gene            1916..>2577
                     /locus_tag="VEW91_11655"
     CDS             1916..>2577
                     /locus_tag="VEW91_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYM92276.1"
                     /translation="MTSCRRSSPRPGRPRASFWGSGLLIPLLALGPVGAAAPPRHAAV
                     EVVVTATAALLMDARTGQVLYDRNAHLIWSPASTTKIMTALVALEGTPLDALITISPD
                     AAHFREGAIVGLPEGARIPLRDLLYALLLASGNDAAVAIAEGVAGTVPAFVDRMNAKA
                     VQLGASQTHFTSPHGLYDPEHYSTAYDLALITRVALQNPAFQQIVHTRVWALAVPGRS
                     PRR"
BASE COUNT          364 a          885 c          914 g          414 t
ORIGIN      
        1 ttcttcgtgt tgttctcgtt tggatgggcg cgcccggttc ccgtcaaccc ccagaacttc
       61 gccaaccccc gtcgtgggat gctcacggtg gccctggccg gcccgctcgc caacatcgtg
      121 gtcgccttcg ccatcggcgg cctcgtgaag gcgccgggcc tcatcgggac cccgtggggc
      181 gatctcgcct ccatgctcgt gtggatcaac gtcgtgctgg ccgtgttcaa cctcatcccc
      241 atcccgccgc tcgatggctc gcggattctc gaggggctcc ttcccagcga gcaggcgatc
      301 gcgtacgcga ggatccagcc gtacggcacg gtgctgatcc tggtgctcct ctacaccggc
      361 gtggtggggc aggtgatgtt tccggcggtg cggtggctgt acggcgcctc caccgggacg
      421 gggttccacc tgtagtggcc cgcccctccc ggcaggaacc ccgggcgaag cggcgaatat
      481 gccggccaga gaagcagcag ccgacgcagc agcagacgga cccctgagcc gaagcagcga
      541 ccggaggacg tatcgtggga taccacgtcc agtctgacac cttcaccggc ccgctcgatc
      601 ttctggtcag cctcgcgcgt cgcgggcagg tggatctccg ggccctatcc ctccgctcca
      661 ttgccgacgg ttacgtggcg caggcccgag gggcgcttga cctcgatgag gccacggagg
      721 tgctggtgga cctcgccgtg ctcgccgacc tcaaggtgcg aaccctggtc ccgaacgcgc
      781 ccccggccga ggaggtcctg gaagaaggcg aaggagcctc ggacctccgg gaccggttgg
      841 gcgcccagat ggccggctac ctcaagttcc gcgaggcggc gttggccctg cgcgtgctgg
      901 aggaggtcca gcgccaggtg ttcgtgcgcg cgcccaacgc ccccgacccg acaggcgagg
      961 tgctggtcga tggcgtgacg ctgcaggacc tgttcgccgc ctttgcccag gtccttcgcc
     1021 gtgctcggga agcgccgcag gagatcgccg gggaagagtt cacggtcgag cagaagatgg
     1081 acgcgctcgt cgccctcctc gaggaggcgc ccggtggggt cgccttcggc gcgctgttca
     1141 gggaaggcgc gagccggctc gagatcatcg tgacgtttct ggcgatgctg gaactcatcc
     1201 ggcggcggcg gatccgggtg cggcaggtgc aggtgttcgg cgagatcgtg gtggcgctgg
     1261 cggtccccgt ttgagggtac attaatgagc gaccccctcg ggccggtgaa ggcgtcggag
     1321 gcggcgccgg ccctgatcca gcaggactgg ccggagggga acgggcacgc ggttgccgtc
     1381 acccccgagg gtccggccgc ggccgatcgg gcggagggcg gtctcgcgat ccgcaacgcg
     1441 gtcgagtgtc tcctcctggc ccggggcggg ccggtccggc tgtcggacct gcgcaaggtg
     1501 ctcgacgtcg aggaggcgga gttgcgggcg accatggcgg ccctccaggt ggagtacgag
     1561 ggccgggggc tccagatcca ggagatcgca gaaggatacc aactgtgcac gaggccggaa
     1621 tacgcagcgg tggtgcagcg gctcctccgc ttgaacgaac tcgagccgct cacccgggcc
     1681 tccctcgagg tgctggcgat cgtggtctac cgacagccgg tgacgcgcgc cgaggtggac
     1741 gcgattcggg gcgtccaatc ggtccaccac ctcgtgaagt tgcaagaccg gcggctgatc
     1801 caggaactgg ggcggcgccc cgggcccggg cggccgatcc tgtacgggac caccgaagga
     1861 ttcctgcggt acttcgggct caaggacctc ggcgcgcttc cgaggattgg agatcatgac
     1921 ctcctgccgg cgctcgtccc cccggcccgg taggccccgc gcgtcgttct ggggatccgg
     1981 cctgctgatc ccgctgctgg ccctgggacc ggtgggggcc gccgcgccac cccgccacgc
     2041 cgcggtcgag gtggtcgtca cggccaccgc ggcgctgctg atggatgcca gaaccgggca
     2101 ggtcttgtac gatcggaacg cccacctaat ctggtctcct gccagtacga cgaagatcat
     2161 gaccgcgctc gtggcgctcg aaggcacgcc gctcgatgcg ctcatcacga tcagcccgga
     2221 tgccgcccac ttccgcgagg gcgcgatcgt gggcctgcct gagggggcgc ggatcccgct
     2281 gcgcgatctc ctgtacgcgt tgctcctggc gtcgggtaac gacgccgcgg tggcgatcgc
     2341 cgagggggtc gccggcacgg tcccggcgtt cgtggaccgg atgaacgcga aggccgtgca
     2401 actcggggcc tcccagacgc acttcacctc tccccacggg ctctacgacc cggaacacta
     2461 ttccacggcg tacgatctgg ccctcatcac tcgggtggcg ctgcaaaacc ccgcgttcca
     2521 gcagatcgtc cacacgcgcg tgtgggcgct cgccgtccct gggagatccc cccgccg
//
LOCUS       DASQGH010000586         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14030, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000586 DASQGH010000000
VERSION     DASQGH010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14030"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VEW91_11660"
     CDS             <1..845
                     /locus_tag="VEW91_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYM92277.1"
                     /translation="ESGRNRVRVYQPDQDSQAPAVARMDWQRRIQEALEEDLFVLTAQ
                     PILDLRNNRTSHYEVLLRMMGAQGKIIPPGVFLDTAERLGLIHGIDQWVVRHAIRLIA
                     AHKKQGRAISLVTNVSARTLADAEIVPLIQRELELSGVDPRFLALEMAESAAITNIDK
                     AGKFAAALKQVGCRVGLDDFGVGFASFHHLKHLPVDYLKIDASFIRDLPRDTTNQQLV
                     RTMVAVARAFGKETIAKFVSDAGTLRLLKEYGVDYAQGFHIGAEAKEITSITVDSASG
                     PRRV"
     gene            complement(808..1356)
                     /locus_tag="VEW91_11665"
     CDS             complement(808..1356)
                     /locus_tag="VEW91_11665"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HYM92278.1"
                     /translation="MPKQRKRARSVPVLTRLPRRGYVIQEKYLRRFRSLAVRPDADFA
                     GLVHMGEDGNEIWVEAGLSEPEKRFTIVHELVHARRQRAEEELRDERLEEAIVELETV
                     ARVNERTLRRMPSGVTLTLLHGYLTGGRRFDPDTRAGLRAIYRKIWTLLGVRRGRSHA
                     ARVAPPRGARLTRAGARWPSLR"
     gene            1438..1917
                     /locus_tag="VEW91_11670"
     CDS             1438..1917
                     /locus_tag="VEW91_11670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYM92279.1"
                     /translation="MIQTPIGAWCWRALPRWGKRALLWLFNTHFIVGTVAVVHDASGR
                     VLVARHTYRARAPWALPGGWVRRGENPSEAVVREIREETALEVEVIAPLTVQMESPVH
                     LTVIYDARLIGGDFRPSPEVAEVRFLDPGAWPEGLRQDHRAILESFGWRPARDRTFA"
     gene            1973..2050
                     /locus_tag="VEW91_11675"
     tRNA            1973..2050
                     /locus_tag="VEW91_11675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2009..2011,aa:Glu,seq:ttc)
     gene            2236..3063
                     /locus_tag="VEW91_11680"
     CDS             2236..3063
                     /locus_tag="VEW91_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYM92280.1"
                     /translation="MFFAIFLVSVVAGIGGAILGLGGGIIIVPALTLLLGIPIRFAIG
                     ASIVAVIATSSGAASAYIRDRLTNLRIGIALELMTTIGALSGAVVAGRVSPRWLYVIF
                     GLLLAYSAIALLGRLKIELPGEVPHDPLADRLRFSGEYYDQALGQRVPYTATGVIPAG
                     AMMYVAGVFSGLLGIGSGLFKVLAMDVAMRLPMKVSTSTSNFMVGVTAAASSGVYFSR
                     GDIHPVIVAPIALGVLTGAWVGARVMGRLRNTTIRKLFMPVLVIMAVSMVLRGLSVP"
     gene            3060..3491
                     /locus_tag="VEW91_11685"
     CDS             3060..3491
                     /locus_tag="VEW91_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HYM92281.1"
                     /translation="MRDDRGRRGEGEPGETGLRPVTSALLRYGVLLAAAVILAGLLLL
                     LIQVGPRAFVSMPRLNAPEAGTDLTSLQVVLRELLPPKPRAAMEAGILLLIATPVVTV
                     GASAVAFALERDWLYALIATGVFAMLILGFALGRGIAMRGG"
     gene            complement(3535..>4226)
                     /locus_tag="VEW91_11690"
     CDS             complement(3535..>4226)
                     /locus_tag="VEW91_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM92282.1"
                     /translation="ALSRAVLDALTRVDRDPGARALIITGEGRAFSTGADLKEREGAT
                     PEAIWAHNRIIFQIPLELERLAVPVIAAINGFALGGGCEVALGCDLRWAASTAEFGCP
                     EVTRGIIPAGGGTQRLPRLVGPSRAMVLILTGQRIGAQEAHRIGLVDAVVVPDDLQRS
                     ATDAARAIAANAPLAVRAAKRAIRHGLRSTLEAGLELEGQLQRLLYASADCQEGIAAF
                     RERRPPRWTGR"
BASE COUNT          599 a         1514 c         1432 g          681 t
ORIGIN      
        1 aggagagcgg ccggaaccgg gtccgcgtct accagccgga ccaggactcg caggcccccg
       61 ccgtcgcccg catggactgg cagcggcgca tccaggaggc gctcgaggag gacctcttcg
      121 ttctcacggc ccagccgatc ctcgacctgc gcaacaaccg cacctcgcac tacgaagtct
      181 tgctccgcat gatgggggcg caagggaaga tcatcccgcc gggcgtcttc ctcgatacgg
      241 ccgagcggct cgggctgatc cacggcatcg accagtgggt cgtgcgccac gcgatccggc
      301 tgattgccgc gcacaagaag cagggccgcg ccatcagcct ggtcaccaac gtctccgcca
      361 ggacgctagc ggacgccgag atcgtcccgc tgattcagcg cgaactcgag ctctcgggcg
      421 tcgatccccg cttcctggcc ctggagatgg ccgagagtgc ggcgatcacc aacatcgaca
      481 aggcggggaa gtttgcggcg gcgctcaagc aggtcggctg ccgggtcggc ctcgacgact
      541 tcggggtggg gttcgcctcg ttccaccacc tgaagcacct gcccgtcgac tacctgaaga
      601 tcgacgccag cttcatccgc gatctccccc gggacaccac caaccagcag cttgtgcgga
      661 cgatggtggc ggtggcccgc gcgttcggca aggaaacgat cgcgaagttc gtgagcgatg
      721 ccggaaccct tcggctccta aaggagtacg gcgtcgacta cgcgcagggc ttccatatcg
      781 gcgccgaggc gaaagagatc acgtccatca ccgtagactc ggccagcggg ccccggcgcg
      841 tgtaaggcgg gccccccgcg gaggggccac ccgcgccgca tggctccggc cacggcggac
      901 cccgaggagc gtccagatct tccggtagat cgcccgcagc cccgcgcgcg tgtcggggtc
      961 gaaccggcgg cccccggtca ggtagccgtg gagcagggtc agcgtcaccc ccgacggcat
     1021 ccgccggagc gttcgttcgt tcacgcgcgc caccgtttcc agttcgacga tcgcctcctc
     1081 cagccgctcg tcccgcagct cctcctcggc ccgctggcgg cgtgcgtgca ccagttcgtg
     1141 cacgatggtg aaccgctttt ccggctcgga gaggccggcc tccacccaga tctcgtttcc
     1201 gtcctcgccc atgtggacca ggccggcgaa atcggcatcg gggcgcacgg cgaggctgcg
     1261 aaaccgccgc aggtacttct cctggatgac gtatccccgg cgcggcaggc gtgtgagcac
     1321 gggcaccgac cgggcgcgct tcctctgctt cggcattcgt tcctccattc aaagtgtgcg
     1381 gcgctcaccg ctcggggtcg ggagggcgat cccccatccc gccgaagtac ctccagtgtg
     1441 attcagaccc cgatcggtgc gtggtgctgg cgcgcgctcc cgcgctgggg aaagcgcgcg
     1501 ctcctctggt tgtttaatac ccatttcatc gtgggaactg tcgccgtcgt gcacgacgcg
     1561 agcggacggg tgctcgtggc ccggcacacc taccgcgccc gcgcaccctg ggcgcttccc
     1621 ggcgggtggg tccgccgggg cgaaaatccg tcggaggccg tggtccggga aatccgcgag
     1681 gagaccgcgc tggaggtcga ggtcatcgcc ccgctcaccg tgcagatgga gagccccgtg
     1741 cacctgaccg tgatctacga cgcgcggttg atcggaggcg acttccgccc gagccccgaa
     1801 gtcgccgagg tccggttcct ggacccgggg gcctggccgg aagggctgcg ccaggaccac
     1861 cgcgcgatcc tcgagtcgtt cggatggcgc cccgcgcgcg accgcacctt cgcgtgatgc
     1921 cctctttgcg gaacgtcccg ggtgtggtag tgtgggagcg ggaagaaacg gcgcccccat
     1981 cgtctagccc ggtctaggac gtcggccctt caagccggca acgcgagttc gaatctcgct
     2041 gggggcacca aagctcccag cgccacacac ccgccctatt cggccgcacc agaggtcacc
     2101 tgcgcagcat cacgcgggcg tgcgtcatca tgcccgcatc ttctccagca ccttcagcgt
     2161 ccttcccggc atgggcgcgg tccgctggca gaggagagcc cccccgcgca tagaatccgc
     2221 ctgcgggaga acacggtgtt ctttgcgatc ttcctggtct ccgtggtggc gggcatcggg
     2281 ggcgcgatcc tcggcttggg cgggggcatc atcatcgtcc cggcgctcac gctcctcctc
     2341 ggcatcccga tccggttcgc catcggcgcc agcatcgtcg cggtgatcgc gaccagcagc
     2401 ggcgcggcgt cggcgtacat tcgagaccgc ctgacgaacc tcaggatcgg gatcgcgctc
     2461 gaattgatga ccaccatcgg cgcgctctcg ggtgctgtgg tcgccgggcg ggtctccccc
     2521 cgttggctgt acgtgatctt tgggctgctc ctcgcctact ccgcgatcgc cctgctcggc
     2581 cggttgaaga tcgaactgcc cggcgaggtc ccccatgacc ccctggcgga ccggctgcga
     2641 ttctccggcg aatactacga ccaggccctc ggccagcggg tcccctacac tgcgacgggg
     2701 gtgatccccg ccggggcgat gatgtacgtc gccggggtct tctctggtct cctggggatc
     2761 ggcagcggat tgttcaaggt gctcgcgatg gacgtcgcga tgcggctgcc gatgaaggtc
     2821 tcgacgtcga cgagcaactt catggtaggg gtcacggcgg ccgccagctc cggagtctac
     2881 ttttcccgcg gcgacatcca cccggtcatc gtcgcgccga tcgcgctggg ggtgctgacc
     2941 ggcgcctggg tcggcgcccg ggtcatgggg cgactaagaa acaccacgat ccgcaagctc
     3001 ttcatgcccg tcctggtgat catggcggtc agcatggtcc tgagggggtt gagcgtgccg
     3061 tgagggacga ccggggacgg cgcggggagg gagaacccgg cgaaaccggc cttcgcccgg
     3121 tcaccagcgc gctcctacgg tacggcgtgc tgctggccgc cgccgtgatc ctcgcggggc
     3181 tgctgctcct cctgatccag gttgggccca gggcgtttgt ctccatgccg cgcctcaacg
     3241 cgccggaggc cggcaccgac ctcacgtccc tgcaggtggt cctccgcgaa ctgctgcctc
     3301 ccaagccgcg ggcggccatg gaggcgggga tcctgctgct gatcgctacc cccgtggtga
     3361 cggtcggagc ctcggcggtc gcgttcgcgc tggagcgcga ctggctgtat gcgctcatcg
     3421 cgaccggggt gttcgcgatg ctgatcctgg ggtttgcgct cggccgcggg atcgcgatgc
     3481 ggggaggcta gccgcgcgaa ggcgctcggt cgggctgccg catcgcctcc cctcctaccg
     3541 ccccgtccat cgcggcggcc ggcgctcgcg gaacgcggcg ataccctcct ggcaatccgc
     3601 gctggcgtag agcaggcgct gcagttggcc ctcgagttcc aaaccggcct ccagggtgct
     3661 ccggagcccg tgccggatcg cccgcttggc cgcccggacg gccagcggag cgtttgcggc
     3721 gatcgcgcgt gcggcgtctg tggccgagcg ctggaggtcg tccggcacca ccacggcatc
     3781 gacgaggccg atccggtgcg cctcctgcgc gccgatgcgt tgcccggtga ggatcagcac
     3841 catcgcgcgc gacggcccca ccaggcgcgg caggcgctgg gttcccccgc ccgcggggat
     3901 gatcccgcgg gtcacctcgg ggcacccaaa ctccgccgtg ctcgcagccc accgcagatc
     3961 gcacccaagc gcgacctcgc acccgccgcc cagtgcaaac ccgttgattg cggcgatcac
     4021 gggcaccgcc agccgctcca gttccagcgg gatctgaaaa ataatccggt tgtgcgccca
     4081 gatcgcctcg ggcgtggcgc cctcccgctc cttgaggtct gcgccggtgc tgaacgctcg
     4141 gccttccccg gtgatgatca gcgcgcgggc tccggggtcc cggtcgacgc gggtgagcgc
     4201 gtccagcacc gcccgggaca gcgcgg
//
LOCUS       DASQGH010000587         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14047, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000587 DASQGH010000000
VERSION     DASQGH010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14047"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1269)
                     /locus_tag="VEW91_11695"
     CDS             complement(205..1269)
                     /locus_tag="VEW91_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92283.1"
                     /translation="MRRLVVSDCGDFKTRYAQEAALFPNDVGRLALGVLLIVWFLIVP
                     AVAGRYVLSVINLTGIAVIGATGLNLLTGYTGQITMGHGAFMGVGAYATAILAARAHL
                     PIWAAIPLAGVVTAGVGAFFGIPSLRLKGLYLAIATLAAQFILEYVFLHWDWLTRGTS
                     GFLVPTARIGGWALRGERPFFWVISATVVLITWLSLNLLRTRVGRAFVAIRDNDRAAA
                     AIGIHLFGYKLLAFFVSAFMVGVAGALYAYYLGVVVPGRFTLDLSIDYLAMGIVGGLG
                     TVLGPFYGAAVITVIPEALRTLSQTLSGVFPNIATALNALRAVVFGLIIVAFLIFEPD
                     GLADRWRVIRAYFKHWPYRY"
     gene            complement(1321..>1517)
                     /locus_tag="VEW91_11700"
     CDS             complement(1321..>1517)
                     /locus_tag="VEW91_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92284.1"
                     /translation="GGLDSVPGAVVGGVAVGVLENLAGGYLDPLVGGGTKEVAPFVVL
                     VLVLMLRPYGLFGTVEIERV"
BASE COUNT          272 a          573 c          492 g          180 t
ORIGIN      
        1 acatcccctg tcggccccgt gagatcgaac agcgccccga gtttcaccgc cggctgctgc
       61 tgggccgcgg cggcgagtgg ccggcccagg accgccaccg cggccccccc tcccagcgtg
      121 cggagcacct cgcgtcgatt catctcccca cctccctccg cgccgtcccg ctcacatggg
      181 gatggcacgg tgctgtccgt tggctcagta cctgtacggc cagtgcttga agtaggcgcg
      241 gatcacccgc caccggtcgg ccagcccgtc gggctcgaag atcaggaagg cgacgatgat
      301 caggccgaag acgaccgccc ggagggcgtt gagagcggtg gcgatgttgg gaaacacgcc
      361 gctgagcgtc tgactgagcg tccgaagcgc ctccgggatg acggtgatga ccgccgcgcc
      421 gtagaacggg ccgaggacgg tccccaaccc gcccacgatc cccatcgcca ggtagtcgat
      481 cgagaggtcc agcgtgaacc tcccgggcac gaccacgcca agatagtagg cgtagagcgc
      541 gccggcgacg ccgaccatga acgcgctcac gaagaacgcc agcagcttgt agccgaagag
      601 gtggatcccg atcgcggcgg ccgcccggtc gttgtcccgg atcgccacaa atgcccgccc
      661 cacccgggtc cggaggaggt tgaggctgag ccacgtgatg aggacgaccg tcgcgctgat
      721 cacccagaag aacgggcgct ccccccgaag cgcccacccg ccgatgcggg cggtcgggac
      781 gaggaatccg gacgtccccc gagtcaacca gtcccagtgg agaaagacat actcgaggat
      841 gaactgcgcg gcgagcgtgg cgatcgcgag atacaggcct ttgagccgca gcgacgggat
      901 gccgaagaac gcccccaccc ccgcggtgac caccccggcg agcgggatcg cggcccagat
      961 cgggaggtgc gcgcgggccg ccaggatcgc cgtcgcgtac gcgccgaccc ccatgaacgc
     1021 cccgtgcccc atcgtgatct ggccggtgta gccggtgagc aaattgaggc ccgtcgcccc
     1081 aatgaccgcg atcccggtca ggttgatgac actcagcacg taccgccccg cgacggcggg
     1141 gacgatcagg aaccagacga tgagcaggac gcccagcgcg aggcggccga cgtcgtttgg
     1201 gaacagcgcc gcttcctgcg cgtaccgcgt cttgaagtcc ccgcagtcgg agacgacgag
     1261 ccgcctcacg gccgcctccc cgccgtcggc cggccccggg cctccacccc gccgccgcgc
     1321 tcacacacgc tcaatctcca ccgtcccgaa cagcccgtac ggccgcagca tcagcacgag
     1381 gacgaggacc acaaagggcg ccacctcctt ggtccccccg ccgaccagcg ggtcgaggta
     1441 gcccccggcc aggttctcga gaacgccgac cgcgaccccg ccgaccaccg cccccgggac
     1501 gctgtccagc ccgccca
//
LOCUS       DASQGH010000588         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50686, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000588 DASQGH010000000
VERSION     DASQGH010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50686"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEW91_11705"
     CDS             <1..536
                     /locus_tag="VEW91_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HYM92285.1"
                     /translation="LPFQDYFVLRGARDPVTAIEFEGLDRARPSPEAVEAVGSARLII
                     VAPSNPLVSIRPILALPGMEAALARSAALRVAVTPLIRGAAIKGPTVEMLRGLGMDAA
                     PDAVAALYRTFLDVFVLDIRDRALAGSVARAGSPGRPLEVECTDTLMLDETGRRRLAR
                     DLLAIAQTRGVEIACRP"
     gene            533..1195
                     /gene="cofC"
                     /locus_tag="VEW91_11710"
     CDS             533..1195
                     /gene="cofC"
                     /locus_tag="VEW91_11710"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HYM92286.1"
                     /translation="MRVGAIVPQKLIRLAKSRLAGALAPEARGALSLALLGTVCAALR
                     AVPAIEEVAVLTPDPLVRAWADARGVRTLVDHGVGLNAALDAALASAVAPSRAILVIA
                     SDLPLLQPVDVATFLSRAGTDTVVLAPSKDGLGTNAMLLPPGIRLRPAYGEGSLLAHR
                     RAAIARGVRTIEVRRPGLALDIDDPVDLLYVGTAAGAVPATIWARSARFDADGGTGTG
                     PS"
     gene            complement(1131..>1903)
                     /locus_tag="VEW91_11715"
     CDS             complement(1131..>1903)
                     /locus_tag="VEW91_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofH"
                     /protein_id="HYM92287.1"
                     /translation="LAELRAAGVGSLPGTAAEILDDEVRDRISPGRIRTAQWIDVITT
                     AHELGIPTTSTMMFGHVETAGHQARHLVLLRDLQRRSGGFTEFVPLSFIHTEAPMWRK
                     QLVPGLRAGPTGAEVVRVHATARIVLNRAIPNLQVSWVKEGPRLSQLLLTAGANDLGG
                     TLINESISTAAGAAYGQLVPPMEMRRLVWDAGRIPAERSTTYQVRRVFEEEASDHPEA
                     LDVAAQETGRFGSYHQLIKMDRYRYRHPHRSAQSAPKS"
BASE COUNT          256 a          668 c          663 g          316 t
ORIGIN      
        1 gcctgccgtt tcaagattat tttgtcctcc ggggcgcgcg ggatccggtg accgcaatcg
       61 agttcgaggg cctcgaccgc gctcgcccgt caccggaagc ggtcgaggcg gtggggtccg
      121 cccggttgat catcgtggcg ccgagcaacc cgctcgtcag tatccggcca atccttgcgc
      181 tcccggggat ggaggccgcg ctcgcccgga gcgccgccct gcgcgtggcc gtcaccccgc
      241 tgatccgcgg tgccgcgatc aaagggccga ccgtggagat gcttcggggc ctgggcatgg
      301 acgccgcccc ggacgcggtc gcggcgctgt accgaacctt tctcgacgtc ttcgtcctgg
      361 acatccgcga ccgtgcgctc gcaggatccg ttgcgcgcgc cgggtcgccg ggacggccgc
      421 ttgaggtcga gtgcacggac acgctgatgc tggacgagac ggggcggcgc cgcctcgccc
      481 gggatctgct tgcgatcgcg caaacccggg gggtcgagat cgcgtgtcgt ccatgagggt
      541 cggcgcgatc gtgccgcaaa agctgatccg tctggcgaag agccggctcg cgggtgcgct
      601 cgctcccgag gctcgaggag ccctcagcct ggcgctcctc gggaccgtgt gcgccgccct
      661 ccgggcggtg ccagccatcg aggaggtcgc cgtgttgacc cccgatccgc tcgtccgcgc
      721 ctgggcggat gcccgggggg tgcggacgct cgtcgatcac ggggtcggac tcaacgccgc
      781 gctcgacgcg gccctggcgt ctgctgtagc gccctcccgg gcgatcctgg tgatcgcctc
      841 ggacctgccc ctgctgcagc cggtggacgt agccacgttt ctgtcccgcg cgggaacgga
      901 tacggtcgtc ctggctccgt ccaaggacgg cctcggaacc aatgccatgc tcctcccacc
      961 gggcatccgg ctccggccgg catacggcga gggcagcctc ctggctcacc gcagagccgc
     1021 gatcgccagg ggggtgcgga cgatcgaagt ccggcgcccc ggcctagccc ttgacatcga
     1081 tgacccggtc gatctcctgt atgtggggac tgccgcgggg gctgttccgg ctacgatttg
     1141 ggcgcgctct gcgcgcttcg atgcggatgg cggtaccggt accggtccat cttgatcagc
     1201 tggtggtaac tgccgaaccg gccggtttct tgggccgcaa cgtccagggc ctccgggtga
     1261 tcgctcgcct cctcctcgaa cacgcgacgg acctggtacg tggtcgatcg ctccgccgga
     1321 atccggcccg cgtcccacac gagccgccgc atctccatcg gcgggacgag ctggccgtag
     1381 gccgcacccg ccgccgtgga aatgctctcg ttgatgagcg tgccgcccag atcgttggct
     1441 ccggcggtaa gcagaagctg gctgagccgc gggccctctt tgacccagga gacctggagg
     1501 ttcgggattg cccggttgag gacgatgcgg gcggtggcat gcacccggac cacttcggcg
     1561 cccgtcgggc ctgcgcgcaa cccggggacg agctgcttcc gccacatcgg ggcctccgta
     1621 tggatgaagc tcagcggcac gaactccgtg aaccctccgg agcgacgctg gagatcgcgc
     1681 agcaggacga gatgacgtgc ctggtggccc gccgtttcga cgtggccaaa catcatcgtc
     1741 gacgtcgtag gaatgccgag ctcatgggcg gtcgtaatca cgtcgatcca ctgcgccgtc
     1801 cgaatccgtc cgggcgagat gcggtcccgc acttcgtcgt ccaggatctc ggccgccgta
     1861 cccggcaggc tccccacccc ggcggccctg agctcggcaa gat
//
LOCUS       DASQGH010000589         7518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_47935, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000589 DASQGH010000000
VERSION     DASQGH010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47935"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VEW91_11720"
     CDS             <1..1460
                     /locus_tag="VEW91_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92288.1"
                     /translation="VNEFRAELAAKNVRLTAVEEELNALKARLDNVKITGRFRWRYDG
                     GPSAGAPFTLNGNPNVGATDASKFATIFRSREGLKLSVDGSVAPDVHTILGLELATNL
                     SSTPLTFNSSNLGTVNLTNTTGGTTTGPQYVLANIVELFFDWKNIWGTPIELWIGRFG
                     GGVQGFASFPVQFGPQGLLLATASDTWGASTGNSGVNLADGLRLAGHFAELADLQIQA
                     VIIRVTGPTGGGTYNLGEDMYGVDANIRIIPGLRGGAYYVANTTNAGSTVTVSPLGAL
                     YHVYGNPDTPSQNPATALCPAVATGALSSTGIAVVQGGITCAAAGSGWGGYVQWDVMP
                     GIHFDGEYAQWTDSVHSISDSAFWTNLIFDLKALLGTDNNFSLQTGYAYAGTNFYAPY
                     GQAFDAGINGSVFPGNFQGFTGVVTYDLFSNLTLLGQYITGNNVSNGMTVSEWQAGFI
                     YKFAPGTTIWIRYETDTIGGIAYYYLYRAEMNYTF"
     gene            1598..1888
                     /locus_tag="VEW91_11725"
     CDS             1598..1888
                     /locus_tag="VEW91_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92289.1"
                     /translation="MRGQTVGVLILIAVLSLFAADVSVGCGQGYVHGTVEQKLVSLSG
                     EPDRQLVINGSIFQVPISFYNEVQVGDMVRFTGGDWTIEKRASAPAPASPSP"
     gene            1893..2483
                     /locus_tag="VEW91_11730"
     CDS             1893..2483
                     /locus_tag="VEW91_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92290.1"
                     /translation="MPRLAAGAVVASVLLSFSVPVGATASGPTVVGVVDLYAPTPLAA
                     FSGLFPERFAADDLSASLARAAAGRFTLVPRDAVEKAASGAGWRGEDVLHFDRLGRLA
                     RIVGADRLVIGWIAALDMSDSGGVGPDNGGTPHMIDATIVVQVFDAAQGRIISETRQM
                     GSAVGAVPSVLAEQALHRAVGAALPSLLGTLISQMP"
     gene            2494..3207
                     /gene="pyrF"
                     /locus_tag="VEW91_11735"
     CDS             2494..3207
                     /gene="pyrF"
                     /locus_tag="VEW91_11735"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYM92291.1"
                     /translation="MDPRDHLIVAMDTASPEAAAAIADRLAGAARWFKVGSELYTAAG
                     PAAVRAFTRRGRVFLDLKYHDIPAAVSGAVAAAARLGIAMCTVHAAGGTAMLRAAREA
                     ADRAVEGAGRERPRIIGVTLLTSDGPDVLHEVGLAGSPREVALRLAHLAREAGLDGVV
                     ASPLEVAGIRAACGPGFLIVCPGIRPGGTARDDQRRVGTPREAIAAGADFLVVGRPIT
                     RAGDPRAAFEEIVGQMAVP"
     gene            3349..3885
                     /gene="hpt"
                     /locus_tag="VEW91_11740"
     CDS             3349..3885
                     /gene="hpt"
                     /locus_tag="VEW91_11740"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HYM92292.1"
                     /translation="MLDDVAEVLIREDELRARVADLGGRISADYDGKAPLLVGILKGA
                     AIFLSDLIRRITIPCMLDFIATSSYGAASESSGIVRILKDLDQSIEGRHVLVVDDIID
                     TGFTMDYLLETLKARYPASLRVCALLDKASRRRRHVPIDYRGFEIPDRFVVGYGLDYG
                     GIYRNLPFICVLKPEVYQ"
     gene            3907..5064
                     /locus_tag="VEW91_11745"
     CDS             3907..5064
                     /locus_tag="VEW91_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HYM92293.1"
                     /translation="MEFIGRGRAARRTYGFDEIALVPASSTVDPADVDLSWRVGDRGF
                     ALPVVASAMDSVVDVPFAIALSRLGGMAVLNLEGVQTRYPDPRDVLGRLAEVPGDQVV
                     GLMQEVYREPVKDDLVARRIAEIRDGGGVPVVSTTPASAGRLGPLAARAGAALLLVQS
                     TVITEQHRSERGQALSLRGLTARTEIPVMAGNCVSYEAALQLLEAGVAALFVGVGPGA
                     ACTSRKVLGVGVSQATAIVDVAAAREEFERRTGRYVPIVADGGIGVGGDIAKAIACGA
                     DAVMVGSLLARAEEAPGRGSHWGMATPHAALPRGTRVHVGTTGTLRDILFGPARVDDG
                     SQNLVEALRTAMGMCGARTLRQMQQAEIIIAPSLPTEGKTLQIAQRVGQGR"
     gene            5066..6682
                     /gene="guaA"
                     /locus_tag="VEW91_11750"
     CDS             5066..6682
                     /gene="guaA"
                     /locus_tag="VEW91_11750"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYM92294.1"
                     /translation="MSRTLAEPARSSAPTPEAAVPEVPRSVEDLIVVLDFGAQYSQLI
                     ARRVRELHVYSLILPYDTALADLLALRPKGIILSGGPASVYAPGAPRCDPGLFAADVP
                     TLGICYGMQLMAHDLGGRTGPAARKEYGRTRLFVDEAEGPAALFHGLERRLLCWMSHG
                     DSVLHLPLGFETLAHSDHSPHAAVADRARRLYGLQFHPEVSHTPWGTEVLRNFLYQVC
                     GCSGSWTMTSFVEQAVEEIRGRIGDARAICALSGGVDSAAAAGLALRAIGGRLTCVFV
                     DHGLLRRGEAEQVVALFRDHFGAPLVHVDARRRFLDRLRGVVDPEAKRNLIGNEFITV
                     FAEEAVRLDRPEILVQGTLYPDVIESGTRTAARIKTHHNVGGLPEVMPFRLVEPLRTL
                     FKDEVRLVARSLGLPEEMVWRHPFPGPGLAIRILGEVTEERLTILRHADAIVAEEIRR
                     SGLSRELWQAFAVLLPVHSVGVAGDARTYGHVVAVRAVTSEDGMTADWARLPSDLLEA
                     ISNRITGEVSGVSRVVYDVSSKPPATIEWE"
     gene            6703..>7518
                     /locus_tag="VEW91_11755"
     CDS             6703..>7518
                     /locus_tag="VEW91_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYM92295.1"
                     /translation="MDSPTTTGPIPEIDALLNPAQRAAVHHGDGPLLVLAGAGAGKTR
                     VLTYRIAALIQARGVPPQRILAVTFTNKAAGEMRERVEHLVGLPVARAVWMGTFHAIC
                     SRILRRSGAPIGVDPRFLIYDTDDQRALLREVMRALDVDDRQFPPAGVLAAIGRAKNE
                     LVDHVAFAARAETHREEMIARLYAGYQRRLDECHALDFDDLLMRTVHLFREHPAVLAE
                     YQDRFRHILVDEYQDTNHAQFTLLSLLARRDRNVCVVGDDDQAIYRWRGADVRN"
BASE COUNT         1090 a         2590 c         2677 g         1161 t
ORIGIN      
        1 tggtgaacga gttccgggcc gagctggccg cgaagaacgt ccggttgacc gcggtcgagg
       61 aagagctgaa tgcgctcaag gcccggctcg acaacgtcaa gatcacgggc cggttccggt
      121 ggcgctatga cggcggcccc agcgcgggtg cgccgtttac gctgaacggc aaccccaacg
      181 tgggtgctac cgacgcatcc aagtttgcca cgatcttccg gtcacgcgaa gggctcaagc
      241 tctcggtgga cggcagcgtg gcgccggatg tgcacacgat tctcggcctc gagctcgcta
      301 caaacctcag cagcacgccc ctcaccttca acagcagcaa cctcgggacg gtcaacctga
      361 ccaacaccac ggggggcacg acgaccggcc cgcagtacgt gctcgcgaac atcgtcgagc
      421 tgttcttcga ctggaagaac atctggggga cgccgataga gctctggatc ggccgcttcg
      481 gcggaggcgt gcaaggcttc gcctcgttcc cggtgcagtt tggcccgcag gggctcctgc
      541 tggccaccgc ctcggacacg tggggagcct cgacaggcaa cagcggcgta aacctcgctg
      601 acggcctgcg cctcgccgga cactttgccg agctcgcgga cctccagatc caggccgtga
      661 tcattcgggt gaccggtccg accggcggag ggacctacaa cctgggcgag gacatgtacg
      721 gcgtcgacgc caacatccgg atcattccgg ggctccgggg cggcgcctac tacgtggcca
      781 acaccaccaa cgcgggaagc acggtaaccg tgagcccgct cggtgcgctc tatcatgtgt
      841 atgggaaccc tgacacccct tcccagaacc cggctacggc gctctgcccg gcggtcgcca
      901 ccggcgcgct cagctcaacc ggcatcgcgg tggtccaggg cggcatcacc tgcgccgctg
      961 cgggctcggg ttggggcggc tacgtccagt gggatgtcat gccagggatc cacttcgacg
     1021 gcgagtacgc gcagtggacc gactccgtgc actcgatcag cgatagcgcg ttctggacca
     1081 acctgatatt tgatctcaag gccctgctgg gcaccgacaa caacttctcg ctccagaccg
     1141 gctacgcgta cgcgggcacg aacttctacg ccccgtacgg tcaagcattc gatgccggca
     1201 tcaacggctc ggtcttcccc ggcaacttcc agggcttcac cggggtcgtt acctatgatc
     1261 tgttcagtaa cctgaccctg ctcgggcaat acatcacggg caacaacgtc tcgaacggca
     1321 tgaccgtctc agagtggcag gccgggttca tctacaagtt cgcgcccggc accacgatct
     1381 ggatccggta tgaaaccgac acgatcggcg gcatcgcgta ctactatctc taccgcgcgg
     1441 agatgaacta cacgttctag cggtaccgtc aaaggcggtc ggtcgggggt ggcctcccgg
     1501 agaatccggg gggccacctg ttattacgat cgaccttcct gcggttgcga acgcccagag
     1561 aggcggatat actggggagg atctcggagg tgccagaatg cggggccaga cagtcggcgt
     1621 gttgatcctc atcgcggtcc tctcgctgtt tgcggcggac gtctcggtgg gatgcggcca
     1681 gggttatgtg cacggcaccg tggagcagaa gctcgtgagc ctgtcgggag agccagaccg
     1741 gcagctcgtc atcaacggct cgatcttcca agtgcccatc tccttctaca atgaggtcca
     1801 ggtgggggac atggtgaggt tcaccggtgg ggactggacg atcgagaagc gggcgagcgc
     1861 accggcgccg gcatcgccgt ccccgtagga ccgtgccgcg actggccgcg ggcgccgtgg
     1921 tggcgtccgt cctgctgagc ttctccgtgc cggtgggcgc caccgcatcg ggccccaccg
     1981 tggtcggggt cgtcgatctg tatgccccga ccccgctggc ggcattttcg ggtctcttcc
     2041 ccgaacgctt cgccgccgac gacctcagcg cgtcgctcgc ccgcgctgcc gccggcaggt
     2101 tcaccctcgt tccccgtgat gcggtcgaga aggccgcatc cggcgccggc tggcgggggg
     2161 aggatgtgtt gcactttgac cgcctgggga ggctcgcccg gatcgtcggg gccgaccgac
     2221 tcgtcatcgg atggatcgcg gcgctggata tgtccgattc gggaggggtt gggcccgaca
     2281 acggcgggac cccccacatg atcgacgcca ccatcgtggt gcaggttttc gatgccgcgc
     2341 aggggcggat catctcggag acgcggcaga tgggctctgc cgtgggcgcc gtcccgtcag
     2401 tgctggccga gcaggcgctc caccgggccg tgggggccgc gctcccgtcg ctcctcggca
     2461 ccttgatttc ccagatgcca tagcgctcgc gtagtggatc cgcgcgatca cctgatcgtg
     2521 gccatggaca ccgcctcgcc cgaggcggcg gccgcgatcg ccgaccgcct cgcgggcgcg
     2581 gcccgatggt tcaaggtcgg ctctgaactg tacacggcgg ccgggccggc ggcggttcgg
     2641 gccttcaccc ggcggggccg ggtgttcctc gacctgaaat accacgacat ccccgctgcg
     2701 gtgtcggggg ctgtggccgc cgccgcccgg ttgggcatcg cgatgtgcac cgtgcacgcc
     2761 gcgggcggca ccgcgatgct gcgggcggcg cgcgaggcgg cggaccgcgc cgtggagggc
     2821 gccggccggg agcggcccag gatcatcggg gtgacgctcc tcacgagtga cggtccagac
     2881 gtgctccacg aggtgggcct ggcggggtcg ccgcgcgagg tggcgctccg gctggcccac
     2941 ctcgcgcggg aagccgggct cgacggggtc gtggcctccc cccttgaggt cgccggcatc
     3001 cgtgcggcgt gtggtccggg attcctcatc gtctgccccg ggatccggcc cggggggacc
     3061 gcgcgtgatg accagcgccg cgtgggcacg ccccgggagg cgatcgccgc cggtgccgac
     3121 ttcctcgtgg tgggacggcc gatcacccgc gcgggggacc cgcgcgcggc cttcgaggag
     3181 atcgtcgggc aaatggccgt cccctaggaa cgaccgcgcg cacaccgcgt gaacacgacc
     3241 ccgggtcgct ccgttgacac cggtgaccgc ccgttctata atgcacgcaa ttacccggcg
     3301 ccgcggacag cggtccgggg tgatgtggag ggcctgcccc gagtggggtt gctggacgac
     3361 gtcgccgaag tgttgatccg cgaggacgag ctccgggctc gggtcgcgga tctcggcggg
     3421 cggatcagcg cggactacga cggcaaggcc cccctcctcg tcgggatcct caagggcgcc
     3481 gcgatcttcc tctccgatct gatccgccgc atcacgatcc cgtgcatgct ggatttcatc
     3541 gcgacgagct cgtacggcgc cgcgagcgag agctcgggga tcgtccgcat cctcaaggac
     3601 ctggaccaat cgatcgaagg gcgtcacgtg ctggtcgtgg acgacatcat cgacaccggc
     3661 tttacgatgg actacctcct ggagaccctc aaagcccggt acccggccag cctgcgggtc
     3721 tgcgcgctgc tcgacaaggc cagccgccgg cgccgtcacg tgccgatcga ctaccggggg
     3781 ttcgagatcc cggaccggtt cgtcgtcggc tacggcctgg actacggcgg gatctaccgc
     3841 aacctgccgt tcatctgcgt cttgaagccc gaggtgtacc agtgacctgc gaaggaggtg
     3901 acccccgtgg agtttatcgg caggggccgg gccgcgcgcc ggacctacgg gtttgacgag
     3961 atcgccctgg tcccggcgtc ctcgacggtc gatcccgcgg acgtcgatct gtcgtggcgc
     4021 gtcggggacc gggggtttgc cctgccggtc gtcgcctcgg cgatggacag cgtggtggat
     4081 gtgccgttcg ccatcgcgct ctcccggctc ggcggcatgg ccgtgctcaa cctggaaggc
     4141 gtccagaccc gctatccgga ccctcgcgac gtcctcggcc ggctcgccga ggtccccggc
     4201 gatcaggtgg tcggcctgat gcaggaggtg taccgggaac ccgtcaagga cgacctggtc
     4261 gcgcgccgga tcgccgagat tcgcgacggg ggcggggtgc cggtggtctc caccaccccc
     4321 gcctccgccg gccggctcgg gccgctcgcc gcgagagcgg gggcggcgct gctgctggtc
     4381 cagtccaccg tcatcaccga gcagcaccgg agcgagcgcg ggcaggcgct gtcgctcagg
     4441 ggcctgaccg cgcgcaccga gattccggtg atggccggca actgcgtgtc ctacgaagcg
     4501 gccctccagc tgctcgaggc gggggtcgcc gcgctgttcg tgggggtggg cccgggggcc
     4561 gcctgcacca gccggaaggt cctcggcgtc ggcgtctcgc aggccacggc gatcgtggac
     4621 gtggcggccg cccgcgagga gttcgagcgg cggaccggcc ggtacgtgcc gatcgttgcc
     4681 gacggcggga tcggcgtcgg gggggatatc gcgaaggcga tcgcgtgcgg ggcggatgcg
     4741 gtgatggtcg gcagcctgct cgcgcgggcc gaggaagcgc cgggccgggg atcccattgg
     4801 ggcatggcca ccccccacgc ggcgctcccg cgcgggacgc gggtgcacgt cggcaccacc
     4861 ggcaccctgc gggacatcct gttcggcccc gccagggtcg acgacgggtc ccagaacctg
     4921 gtcgaggcgc tgcgcacggc gatgggcatg tgcggggcgc ggacgctccg ccagatgcag
     4981 caggcggaga tcatcatcgc gccctcgctt ccgaccgagg ggaagacgct gcagatcgcc
     5041 cagcgggtcg ggcaggggcg ctgagatgag ccggaccctc gccgaacccg cccggtcgtc
     5101 ggccccgacc ccggaggccg ccgtcccgga ggtcccccgc tcggtcgagg atctgatcgt
     5161 cgtcctggac ttcggggcgc agtacagcca gttgatcgcc cggcgggtcc gcgagctgca
     5221 cgtgtacagc ctcatcctgc cgtacgacac cgcgctcgcc gacctgctcg cgctgcggcc
     5281 caaggggatc atcctctcgg gcggaccggc cagcgtctac gccccggggg cgccccggtg
     5341 cgatccgggg ttgtttgccg cggacgtgcc gaccctgggg atctgctacg gcatgcagct
     5401 catggcgcac gatctcggag ggcgcacggg acctgcggcg cgcaaggagt acggccggac
     5461 ccggctgttc gtggacgagg cggagggccc ggccgcgttg ttccacggcc tggaacggcg
     5521 cctcctgtgc tggatgagcc acggggactc ggtgctccac ctcccgctgg ggttcgagac
     5581 cctggcccac tccgaccaca gcccccacgc ggcggtcgcg gatcgcgcgc gccggctgta
     5641 cggcctgcag ttccaccccg aggtgtccca cacgccgtgg gggaccgaag tgctccgcaa
     5701 cttcctctac caggtctgcg gctgctccgg atcgtggacg atgacctcct tcgtcgagca
     5761 ggcggtcgag gagatccgcg ggcggatcgg cgatgcgcgg gcgatctgcg ccctctccgg
     5821 cggagtggac tccgccgcgg cggccgggct cgcgctccgg gcgatcggcg ggcggttgac
     5881 ctgcgtcttc gtcgaccacg gcctgctgcg gaggggcgag gccgagcagg tcgtggccct
     5941 gttccgcgac cactttgggg cgccgctcgt ccatgtggat gcgcggcggc ggttcctcga
     6001 ccggctgcgg ggggtcgtgg accccgaagc gaagcggaac ctcatcggca acgagttcat
     6061 caccgtcttc gcggaggagg ccgtccggct cgaccggccc gagatcctcg tccagggcac
     6121 gctctacccg gacgtgatcg agagcggcac ccggacggcg gcgcggatca agacccacca
     6181 caacgtgggc gggctgcccg aggtgatgcc gttccggctg gtcgagccgc tccggacgct
     6241 gttcaaggac gaggtccggc tcgtcgcccg ctcgctgggg ctccccgagg agatggtgtg
     6301 gcggcacccg tttccgggcc ccgggctcgc catccgcatc ctgggcgagg tgaccgagga
     6361 acgcctcacc atcctccggc atgccgacgc gatcgtcgcc gaggagatcc gccggagcgg
     6421 cctcagccgg gagttatggc aggcgtttgc cgtcctcctg ccggtccaca gcgtgggcgt
     6481 cgccggagac gcccgcacct acgggcacgt ggtggcggtc cgcgccgtca cgagcgagga
     6541 cgggatgacg gcggactggg cgcggctgcc ctcggatctg ctcgaggcga tcagcaaccg
     6601 catcaccggc gaggtctcgg gcgtcagccg ggtggtctac gacgtcagca gcaagccgcc
     6661 tgctacaata gagtgggaat gacgggcagg caaggaggca ccgtggactc tccaacaacc
     6721 acgggcccga tccccgagat cgacgcgctc ctcaaccccg cgcaacgcgc tgcggtccac
     6781 catggcgatg ggccgctcct ggtgctggcc ggcgccggcg cgggcaagac ccgggtcctg
     6841 acctacagga tcgccgcgct catccaggca cggggggtcc cgccgcagcg gatcctcgcc
     6901 gttacgttca ccaacaaggc cgcgggcgag atgcgcgagc gggtggagca cctcgtgggc
     6961 ctcccggtcg cccgggccgt gtggatgggg acctttcatg cgatctgcag ccggatcctc
     7021 cgccggtcgg gggcgccgat cggggtcgat ccccggttcc tcatctacga taccgacgat
     7081 cagcgggcgc tcctgcgcga ggtgatgcgc gcgctcgacg tggacgaccg gcagtttccc
     7141 ccggccggcg tgctggccgc gatcggccgc gcgaagaacg aactggtcga ccatgtcgcg
     7201 ttcgcggccc gcgcggagac ccaccgggag gagatgatcg ccaggctcta cgccggctac
     7261 cagcgccggc tggacgaatg tcacgcgctc gacttcgacg acctgctgat gcggacggtg
     7321 cacctcttcc gcgagcatcc ggcggtgctc gccgagtacc aggaccggtt ccggcacatc
     7381 ctggtcgacg aataccagga caccaaccac gcgcagttca cgctcctgag cctgctcgcc
     7441 cgccgggacc ggaacgtgtg cgtcgtgggg gacgacgacc aggcgatcta ccggtggcgc
     7501 ggggccgatg tccggaac
//
LOCUS       DASQGH010000590         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_10946, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000590 DASQGH010000000
VERSION     DASQGH010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10946"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="coaD"
                     /locus_tag="VEW91_11760"
     CDS             complement(<1..370)
                     /gene="coaD"
                     /locus_tag="VEW91_11760"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYM92296.1"
                     /translation="MKENRLAVAVYPGSFDPVHNGHLDIIDRARKMFRRVIVGVAENV
                     DKEPMFGVADRVAMLRAAAERAGVAVTSFEGLTVEFVHRMGARVIVKGLRAMMDFENE
                     LKMAAMNKRLRPDIETVFMMT"
     gene            complement(367..933)
                     /gene="rsmD"
                     /locus_tag="VEW91_11765"
     CDS             complement(367..933)
                     /gene="rsmD"
                     /locus_tag="VEW91_11765"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HYM92297.1"
                     /translation="MRPSGTARGRRQRGRGRAGVRPTTRRVTDALFNSLGPRLADARV
                     LDLFAGTGRLGMEALNRGAREAVFVERDPRSAADIRDTLAAAGLSDRAEVRRGNALTG
                     VLALAAEGRRFELIVLDPPYGEGLQRETLRRLAATTLLAEGGLIVAEGHWRDDPGDVP
                     GLERVRESRYGETAMWFYATSAKREEGG"
     gene            complement(936..>2982)
                     /gene="recG"
                     /locus_tag="VEW91_11770"
     CDS             complement(936..>2982)
                     /gene="recG"
                     /locus_tag="VEW91_11770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYM92298.1"
                     /translation="ALQLARLGVATARDLLYHLPRRLDDRSHLRSIYDLQHGATETVQ
                     GTVGQVRQFRPRRRRLIITKAGITDDSGVLQAVWYNQPYLARLLTPGRRLILHGRVQR
                     QSGEIQMIAPEFEPLDDGEDTLHVGRIVPVYPSTDGLSQRALRTIVFHALDDHAPHVG
                     EWLPDRLRRRHRFPDLGAALRQAHFPETLASQAQAHRRMVYEELLLLQLVLLQQKAQA
                     EAEPRAVRYGDPGDLVARFHHSLPYPLTHAQRRVIAEIAADLGRPHPMNRLLQGDVGS
                     GKTVVAATAMLRCAGGGAQGALMAPTEILAGQHYLTLRTLLEPLGATVLLLVGGLGRA
                     ARLEALQRTRDGSADIVVGTHALIEEDVEFHRLGLVVVDEQHRFGVGQRAALREKGHR
                     PDVLVMTATPIPRTLALTLYGDLDVSTLDELPPGRSPIKTYARPTARRPQVYEFVRSQ
                     VAEGRQAYIVCPLIEESEKLQAEAAVGLAARLHKDVFRGLRVEVLHGRMRVEEREAAM
                     RALRAGAIDILVATTVIEVGIDIPNASVMVIEDADRFGLSQLHQLRGRVGRGAHQAYC
                     ILIADPKADDDVAAARLQAMVDTTDGFRIAQRDLELRGAGELLGDRRGGGLRQHGLTD
                     LRVANLLRDHEWLERARQDAREVLAQDPALKHPDHRSLADALRHRFGRAGAENARVG"
BASE COUNT          403 a         1099 c         1017 g          463 t
ORIGIN      
        1 cggtcatcat gaacacggtc tcgatgtcgg gccggagccg cttgttcatc gccgccatct
       61 tcagctcgtt ctcgaagtcc atcatcgccc gaagtccttt gacgatcacc ctggccccca
      121 tccggtgcac aaactcgacc gtgagcccct cgaaactggt gaccgcgacc cccgcgcgct
      181 ccgccgcggc ccggagcatc gccacgcggt cggcgacccc gaacatcggc tccttgtcca
      241 cgttctcggc cacccccacg atgacccggc ggaacatctt ccgcgcgcgg tcgatgatat
      301 cgaggtgccc gttatggacc ggatcgaaac tcccggggta cactgcaacg gcgagcctgt
      361 tctccttcaa ccgccttcct ccctcttcgc ggatgtcgcg tagaaccaca tcgcagtctc
      421 tccgtaccgc gattcgcgaa cgcgttccag cccgggcacg tctcccggat cgtcccgcca
      481 gtggccttcg gcgacgatta ggcctccctc ggcgaggagc gtggtggccg ccaatcgcct
      541 gagcgtttcc cgctggagcc cctcgccgta cgggggatcg aggacgatga gctcgaacct
      601 ccgcccctcc gcggcgaggg ccagcacccc ggtgagcgcg ttcccccggc ggacctcggc
      661 gcggtcgctc aacccggccg ccgccagggt gtcccggatg tctgcggcgc tgcgcgggtc
      721 gcgctcgacg aacaccgcct cccgggcgcc gcggttgagc gcttccatcc ccaaccgtcc
      781 ggtccctgcg aagagatcga ggacccgcgc gtccgccagg cgcggtccga gggagttgaa
      841 cagggcgtcg gttacccggc gcgtggtggg gcggaccccc gccctccccc gtccgcgctg
      901 gcgtctcccc cgggcggtgc cgctcgggcg catcgctatc cgacccgggc gttttccgcc
      961 ccggcgcggc cgaatcggtg cctcagggca tccgccaggc tccggtggtc cgggtgcttg
     1021 agcgccggat cctgcgcgag gacctcacgc gcatcctggc gcgcgcgctc cagccactca
     1081 tgatcccgga ggagattggc gaccctgaga tcggtgagtc cgtgttgccg caaccccccg
     1141 ccccggcggt ccccgaggag ttcgcccgcc ccccgaagct cgaggtcccg ctgcgcgatc
     1201 cggaacccgt cggtcgtgtc gaccatcgcc tgcagcctgg ccgccgccac gtcgtcgtcg
     1261 gctttcgggt ccgcgatgag gatgcagtac gcctgatgcg cgccccggcc gacccgcccc
     1321 cggagctggt ggagctgcga caggccgaac cgatccgcat cctcgatgac catcaccgag
     1381 gcgttcggaa tgtcgatgcc gacctcgatg acggtggtgg ccacgaggat gtcgatcgcc
     1441 cccgcccgga gcgcgcgcat cgccgcctcg cgctcctcca cccgcattcg cccgtgcagc
     1501 acctccacgc gcaggccgcg gaacacgtcc ttgtgcaacc gggcggcgag cccgaccgcg
     1561 gcctccgctt ggagcttctc ggattcctcg atcagcgggc agacgatgta ggcctgccgg
     1621 ccctctgcca cctgcgagcg gacgaactcg tacacctgcg ggcgccgggc ggtcggccgg
     1681 gcataggtct tgatcgggga tcggccgggg ggcagttcgt cgagggtaga gacgtcgagg
     1741 tcgccgtaga gcgtcaacgc gagcgtccgg gggatgggcg tcgcggtcat gaccaggaca
     1801 tccggccggt gtcccttctc ccggagggcc gcccgctggc cgaccccgaa ccggtgttgc
     1861 tcgtcgacca ccacgagccc gagccggtgg aactccacat cctcctcgat cagcgcgtgg
     1921 gtcccgacga cgatgtccgc gctgccatcc cgggtccgtt gcagcgcttc caggcgggcg
     1981 gcgcgcccca agcccccaac gaggaggagc acggtcgccc cgagcggctc gaggagggtg
     2041 cggagcgtga ggtaatgttg cccggcgagg atctcggtgg gggccatcag cgcgccctgg
     2101 gcgcccccgc cggcgcaccg gagcatcgcg gtcgccgcca cgaccgtctt cccgcttccg
     2161 acatccccct gcaggaggcg gttcatcggg tgcggcctcc cgagatccgc cgcgatctcg
     2221 gcgatgaccc ggcgctgcgc gtgggtgagg gggtacggca ggctgtggtg gaatcgggcg
     2281 acgaggtcgc ctgggtcccc ataccgcacc gcccggggct cggcctccgc ctgggctttc
     2341 tgttggagga gcaccagttg caggagcaac agttcctcgt acaccatccg ccggtgggcc
     2401 tgggcctggc tggcgagcgt ctcgggaaag tgggcctggc ggagcgcggc ccccaggtcc
     2461 ggaaaccggt ggcggcggcg gagccggtcg ggcagccact cccccacgtg cggtgcatgg
     2521 tcgtcgagag cgtggaacac gatcgttctc agagcccgct ggctcagccc gtcggtgctg
     2581 ggatacaccg ggacgatccg ccccacgtgc agcgtgtcct ccccgtcgtc gaggggctcg
     2641 aattccggcg cgatcatctg gatctctccc gattggcgct ggacccgtcc gtggaggatg
     2701 agccgccgcc ccggtgtgag caggcgcgcg aggtacggtt gattgtacca gaccgcctgc
     2761 agcacccccg agtcgtccgt gatccccgct ttggtgatga tcagccgccg ccggcgcggc
     2821 cggaactggc gcacctgccc gacggtaccc tgaaccgttt ccgtggcgcc gtgctggaga
     2881 tcgtagatcg accgcaggtg gctacggtcg tcgagccgcc gggggagatg gtagagcagg
     2941 tcccgggcgg tcgcgacgcc cagccgggcg agctggagcg cg
//
LOCUS       DASQGH010000591         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_31022, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000591 DASQGH010000000
VERSION     DASQGH010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31022"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEW91_11775"
     CDS             <1..424
                     /locus_tag="VEW91_11775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="catalyzes the interconversion of D-ribulose
                     5-phosphate to xylulose 5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYM92299.1"
                     /translation="SLAVHTEACPHLYQTIRRIHALGLPPWVALNPATSLSEIEWVLT
                     AVAGVLVMTVEPGSGGQPFIPEMLAKVRALAETRRARDLHFEIAVDGGIGPDTAPAVV
                     EAGASVLVAGTAIFRAPDGIGPAIARLRDAARSVRRRP"
     gene            421..1467
                     /locus_tag="VEW91_11780"
     CDS             421..1467
                     /locus_tag="VEW91_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HYM92300.1"
                     /translation="MTGHRPHLIAHPEYRRHITSPDHPERPERLEAIEALLRKTPLWD
                     ACDHPEPEPVDEEILAQVHTRQYIRTVREIAASGGGLLDPDTPVGPVSYAVARRAVGG
                     ALLAVDCVAGCEGGTAFALLRPPGHHAGEAVGRGFCLFNNAALAARRALEAHGAQRVF
                     ILDWDVHHGNGTQEIFYRDPAVVLCSLHQEAWYPGTGALEETGAGEGEGTTINIPLPA
                     GIGDGGYRHLFEEVVLPLLRAVGPGLIVISAGYDAHHADPLGGMAVTAQGFGRLAQLV
                     RETAGSTPIVALLEGGYDLRALAASVVATLEGLTGIEARLPPQRGAVDEAPFAVASAR
                     ARAVRRIAGQHWAL"
     gene            1584..>1811
                     /locus_tag="VEW91_11785"
     CDS             1584..>1811
                     /locus_tag="VEW91_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYM92301.1"
                     /translation="MKAISVRDARSKLKTLLDRVAAGEEIVIVRRGKAAARLVPPARK
                     RRRLPDLTAFRATIRLKGEPLSITIAKAREED"
BASE COUNT          250 a          631 c          675 g          255 t
ORIGIN      
        1 ttcgctggcg gtccataccg aggcctgccc gcacctctac cagacgatcc gccggatcca
       61 cgcgctcggg ctccctccct gggtcgcgct caatcccgcc acctcgctca gcgagatcga
      121 gtgggttctc accgccgtgg ccggcgtgct cgtgatgacg gtcgagccgg gaagcggcgg
      181 gcaaccgttc attcccgaga tgctcgcgaa ggtccgcgcc ctggccgaga cgcggcgggc
      241 gcgcgatctc cacttcgaga tcgcggtcga cggggggatc gggcccgaca ccgcgcccgc
      301 ggtggtcgag gccggggcgt ccgtcctggt ggccggaacc gcgatcttcc gcgcccccga
      361 tgggatcggg ccggcgatcg cccggttgcg cgacgcggcc cgctccgtcc ggcggcgtcc
      421 gtgaccggcc accgcccgca cctcatcgcg cacccggagt atcgccggca catcacctct
      481 cccgaccatc cggagcggcc ggagcgcctc gaggcgatcg aggcgctgct ccggaagacg
      541 ccgctgtggg acgcgtgcga ccatccggag cccgagcccg tcgatgagga gatcctcgcc
      601 caagttcaca cgcggcagta catccggacg gtccgggaga tcgcggcctc cgggggcggg
      661 ttgctcgatc ccgacacgcc ggtcgggccg gtgtcctatg cggtggcgcg gcgcgcggtg
      721 ggcggcgccc tgctcgcggt ggactgcgtc gccggctgcg aggggggcac ggcgttcgcc
      781 ctcctccgac ccccgggtca ccacgcgggg gaagccgtgg gaaggggatt ctgcctcttc
      841 aacaacgcgg cgctggcggc ccggcgcgcg ctggaggcgc acggggccca gcgggtgttc
      901 attctggatt gggacgtgca ccacgggaac gggacccagg agatcttcta ccgggacccg
      961 gcggtggtgc tctgttcgct ccaccaggaa gcgtggtatc ccgggaccgg ggcactcgag
     1021 gagaccgggg ccggcgaggg cgaggggacg acgatcaaca tcccgctgcc cgcagggatc
     1081 ggggacggcg ggtaccggca cctgttcgag gaggttgtgc tcccgctgct ccgcgcggtc
     1141 ggccccgggc tcatcgtgat ctccgcgggg tacgatgcgc accacgccga tccgctcggc
     1201 gggatggcgg tgaccgcgca ggggtttggc cggctggcgc agctcgtgcg ggagacggcc
     1261 ggatcgacgc ccatcgtcgc gctgctcgaa ggggggtacg atctgcgcgc cctcgccgcc
     1321 tcggtggtcg ccaccctcga ggggttgacc gggatcgagg cccgcctacc cccgcagcgc
     1381 ggagcggtgg acgaagcccc gttcgccgtc gcaagcgccc gagcccgcgc cgtccgccgg
     1441 atcgccggcc agcactgggc gctgtagccc cgcgagagct ccatgcgtcc tgctgagggc
     1501 gtacaggtgt acaggttgac cgtcgcagac ctctcgtgta cacttggtat gtacatatct
     1561 agtggggcgg gaggtctccg gttgtgaagg ccataagcgt gagggacgcc aggagtaagc
     1621 tgaagacgct gctagaccgc gtggccgcag gggaggagat cgtcatcgtc cggcggggga
     1681 aggccgcggc ccggctcgtc ccgccggcgc ggaagcggcg gcggctgccg gatctcaccg
     1741 cattccgggc cacgatccga ctcaagggcg aacccctgag catcaccata gcgaaggccc
     1801 gagaggagga c
//
LOCUS       DASQGH010000592         7155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5336, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000592 DASQGH010000000
VERSION     DASQGH010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5336"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VEW91_11790"
     CDS             <1..479
                     /locus_tag="VEW91_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYM92302.1"
                     /translation="TGYYTDATNLIVSSPPLFVPMNVGHAIVTGGSIEVVGRLAAQWV
                     VRANFTAQQARDAATNLDVIYAPRQLANVEVGYEWAPGSTVTAIATYVGDRFADPANT
                     LPVLGYWLVGLNATWALGEGYTLQAGINNLFDVTYQESLGFPEPGRTMFVGVTKTF"
     gene            843..1325
                     /locus_tag="VEW91_11795"
     CDS             843..1325
                     /locus_tag="VEW91_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYM92303.1"
                     /translation="MQGAPSARAGGGAGAGAGSTAGIGSGSGTGPGVAGEASPAAGAP
                     PIPSEIAQPHVPRLIPPKVITSAGTEYPVDAFRLTVRRQDLGPALAFDGAEGTVGVRA
                     LVLADGTVGRIEVSISSGSEALDRAATETVRRWRFLPATRDGAPIDAYVTVKVRYVVR
                     "
     gene            complement(1310..1861)
                     /locus_tag="VEW91_11800"
     CDS             complement(1310..1861)
                     /locus_tag="VEW91_11800"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HYM92304.1"
                     /translation="MRFGERLGFWIGALPYGLIASGAVLRITPHPANFAPIGAMALLS
                     GAVLPGGWAFAVPLAALVFSDAVLGFYPGWIWVYGSFALIVLIGMALRTRRTVLRVAG
                     AALGSSVLFFVVTNFGEWLGPLYPHTVAGLRADFAAAIPFFRNTALSDLAYALAFFGI
                     YDSAVHLARRRATRLASAVHRTT"
     gene            1971..2987
                     /locus_tag="VEW91_11805"
     CDS             1971..2987
                     /locus_tag="VEW91_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HYM92305.1"
                     /translation="MAFTVIVTDHEFPDLELEERVLRPLGIQLDVLTAPTRSALRSAL
                     GRADAVINEALPLDAELVAAMPRCLLIVRYGVGCDHVDLAAATQAGICVANVPDYGTQ
                     EVAIHTISLLLALHRRLLQYDAALRQGRWLRGPQVVPPIPRLKGLTLGIVGLGRIGRA
                     VAAHAGSLGLHRLTYAPSVTRSAAMDAGAVLVDYGTLLRQSDIVTFHTSLTAATRHLL
                     GAPEIEQMKPGAVVINTSRGAVVDTTALAKALHAGRLAGAGIDVFEQEPLRPTHPLRT
                     APNTILTPHVAWYSEEATQTLRRRVAGEVARVAQGEWPQSLVNPEVRATARLRAGVRM
                     SGPS"
     gene            3070..3411
                     /locus_tag="VEW91_11810"
     CDS             3070..3411
                     /locus_tag="VEW91_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92306.1"
                     /translation="MVRVLAIAFGAVTALALLASLTPVGQVMLGLRPAAQATSVTVIE
                     DKPAEIPGASPASPTQPPGARSPQGGPGVVGNVANILLNLPQILDQTRVGPAPLETKP
                     DVHQESKGAGE"
     gene            complement(3510..3965)
                     /locus_tag="VEW91_11815"
     CDS             complement(3510..3965)
                     /locus_tag="VEW91_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92307.1"
                     /translation="MAVRGVRILVLGIIVGGLVGVFLVGGKAAAWGWRAGAAASGHDV
                     ILLPANPAQGMPFNPREFIPLPNPGQDQGPGAPTPGTGGQDCDRILYFYQGKLYQLRP
                     GPMPRGGNPEFYYMQPYEGPQIPGFPTPGPMAPQMPGPGLPDQVAPRHI"
     gene            4084..4509
                     /locus_tag="VEW91_11820"
     CDS             4084..4509
                     /locus_tag="VEW91_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92308.1"
                     /translation="MALLFTFACGRCGRKYSVYYPKAFIASVYGTMTPAQAGREDADE
                     VASGAIDAARRRAEAAGNTWVDVSQQSKVTCVCGKNLNLNLADHPRVPQRKAATGARQ
                     TGVIAFPEMPKKRHDKGPDGSGGPIPVVPPEGGPPRSGV"
     gene            complement(4460..5761)
                     /locus_tag="VEW91_11825"
     CDS             complement(4460..5761)
                     /locus_tag="VEW91_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYM92309.1"
                     /translation="MTAGSEVAALLAVVPRWIVASVEGNDLDAVEEIELDLGRPPRLH
                     FHDHDEPVTLAREVSREDLQYVLGRVTRFREDNRTGIDRTLHRIACIRDRYHEIVGFT
                     FRIGRTIGEVAQPLQELLESRRNVLVVGRPGAGKTTVLRSAAAILSDRLHRRVVIADT
                     SNEIGGDGQVPHPAIGSARRLQIPLLDHDRPADAGARQAGVILQAVVNHRAETIIVDE
                     LGFAADAQVARTIARRGIQLIATAHGASLRDVLFNTELACLVGDLQTIVLSPEEIERR
                     GMVRHTILERTGPPVFDCAVEIVRRGLFVIHPDVTASVDNLLRGLPPAVLVRPQPPGG
                     RGADRILLPRRQTEGPRSRRRAARVERGVVKWFDSRMGYGFVVDAAGRDVFLHRRVLR
                     RAGRRRLDPGEIVDYEAEVTPRGIKITRLIVVGRPPAARPE"
     gene            complement(5777..6688)
                     /locus_tag="VEW91_11830"
     CDS             complement(5777..6688)
                     /locus_tag="VEW91_11830"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HYM92310.1"
                     /translation="MSALGTGLFCVVLVALASGGGSPASGAGPATLTVYIAGGTTRTA
                     LRLQPGFATVLRADRRVDTVAIGDPRLVTATTVKRGGDVYDLVLQPQAATGTTNMIVW
                     FADLTSIWDLTIGPGPRTTDLVYVVTTPPPNTRTSPPLVQATLPQPSTPPAGPQASTP
                     DLRANPRPERPEYLEARQTFGEAAGVFQLFRGPGGIKIRYRITNTSATDLSVRPNGVL
                     IRANGVLVPFGMSRENADRSRPAVLPPGATESGMINASVPAPRQVEVIFSLFRAKEDR
                     QSPGGTLPAVFQLLFAGLDRLPASSAP"
     gene            complement(6685..7014)
                     /locus_tag="VEW91_11835"
     CDS             complement(6685..7014)
                     /locus_tag="VEW91_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92311.1"
                     /translation="MRTKIAKMGIHVPRDLHRRFRARLILEGRLMQDVIRDWVDEEVS
                     AHRMRPPASGGLRFFKGVDRSTLAALAVPIDQDKIRRLKSKLLMEGRNVSQWGPERME
                     QFLKERA"
BASE COUNT         1074 a         2517 c         2501 g         1063 t
ORIGIN      
        1 tcaccggata ctacacggat gccaccaatc tgatcgtctc ctccccaccg ctgtttgtcc
       61 cgatgaacgt cggccacgcc atcgtcaccg gcggatccat cgaggtcgtg ggccggttgg
      121 ccgcgcagtg ggtggtgcgc gcgaacttca cggcgcaaca ggcgcgcgat gccgcgacga
      181 acctggatgt gatttacgcg cctcgccagc tggcgaacgt cgaggtgggt tacgagtggg
      241 ctcccggcag cacggtcacg gcgatcgcga cctatgtcgg agatcgcttc gccgatcccg
      301 caaacacgct tccggtcttg gggtactggc tcgtgggcct caacgcgact tgggcgctgg
      361 gcgagggcta tacgctgcag gccggaatca acaacctgtt cgatgtgaca taccaggagt
      421 cgctggggtt tcccgagcct ggcaggacga tgttcgtggg cgtcacgaag accttctagg
      481 tgtcgtcgat gcgccctgga acaaagttcc gcctcctggc cgcggtggtg gtagcggtcc
      541 tcggtctggc cctcggccgg ggagcctggc ggagcgcgcc cgggcgggca gtcccgcccg
      601 ccagcccggg cgcgccttcc atggcgcaac tgattcccgg cccgaccgcc gccctggagg
      661 gggaagacgt atcgccggct gggcgcgcag ccgcgagcgc accgcagccg gcgccgcctc
      721 cgcccgcccg gcagggaact ccgctccgtc cgccggcgcg caacgtgcca tcgcgcgcag
      781 cgcagcgggt cccccctccc cgcccgcccg gggccgcgcc gcccagcgcg cccggtggct
      841 cggtgcaggg cgctccatcc gcccgcgccg ggggcggggc gggagcaggg gccgggtcga
      901 cggccgggat cggaagtggt tccggcaccg ggccgggggt cgccggcgag gcttcgcccg
      961 cggcgggggc gccgccaatc ccgagtgaga tcgcccagcc acacgttcca cgcctgatcc
     1021 cgccgaaggt catcacgtcg gcggggacgg aatacccggt cgacgccttc cgcctgaccg
     1081 tgcgccggca ggaccttgga cccgcgctcg cgttcgacgg ggcggagggc actgtggggg
     1141 tgcgcgcctt ggtgctggca gacggcacgg tcgggaggat cgaggtgtcg atctcctcgg
     1201 ggtcagaagc gctggaccgc gcggccaccg agacggtgcg tcggtggcgc tttctgcccg
     1261 cgacgcgcga cggcgccccg atcgatgcct acgtgacggt caaggtccgt tacgtggtcc
     1321 ggtgaacggc tgaggcgagc cgggtggccc gccggcgggc gaggtgcacg gcgctgtcgt
     1381 agatcccaaa gaatgcgagc gcgtaggcga ggtcgctcag cgcggtattc cggaagaacg
     1441 ggatcgccgc ggcgaaatcg gcgcgcaggc cggcgaccgt gtgcgggtag agcggcccca
     1501 gccactcgcc gaagttcgtg acgacaaaga agagcaccga ggatcccagc gccgcgccgg
     1561 cgacgcgcag cacggtccgc cgcgtgcgaa gcgccatgcc gatcaggacg atgagcgcga
     1621 aactgccgta cacccagatc catccggggt agaatcccag caccgcgtcg gagaagacca
     1681 gcgcggccag cggcacggca aacgcccacc ctcccggcag gacggcgccg ctcaggagcg
     1741 ccatcgcgcc gatcggggca aagttggccg ggtgcggcgt gatgcgcagc accgcgcccg
     1801 atgcgatcag gccgtacggc agcgccccga tccagaaccc cagccgttcc ccgaatctca
     1861 tggccgactc acccggtaca gtatacggtc ccgccccggc ccagtcctct cccgctgccg
     1921 cccgcttcgg cggagaggat ggcgccgatc cgggggcaaa tgcgttcggc atggcgttca
     1981 cggtgatcgt caccgatcac gagtttccgg acctcgagct tgaggagcgt gtgctgcgtc
     2041 ccctggggat ccagctcgac gtgctcaccg cccccacccg gtccgcgctg cggtccgccc
     2101 ttgggcgagc cgacgccgtg atcaatgagg ccctccccct ggacgcggaa ctggtcgcgg
     2161 cgatgcccag gtgcctgctg atcgtccggt acggcgtggg gtgcgaccac gtggatctgg
     2221 ccgcagccac ccaggcgggg atctgcgtgg cgaacgtgcc ggactacggg acgcaggagg
     2281 tggcgatcca cacgatctcc ctgctgctcg ccctccaccg gcgcctcctc cagtacgatg
     2341 ccgcgctccg ccagggccgg tggctccggg gcccgcaggt ggttcccccg atcccccgcc
     2401 tcaaggggct caccctcggc atcgtcggcc tcgggcggat cggccgggcc gtcgccgcgc
     2461 acgccggatc cctcggattg caccggctga cctatgctcc gtccgtcacc cggtccgcgg
     2521 ccatggacgc cggcgcggtc ctggtcgact acggaacgct gctccggcag tcggatatcg
     2581 tcaccttcca cacgtcgctg accgcggcga cccggcacct gctcggtgcg cccgagatcg
     2641 agcagatgaa gccgggcgcg gtcgtgatca acacctcgcg cggggccgtc gtggacacga
     2701 ccgcgctggc caaagcgctg cacgcgggca ggctggccgg cgcggggatc gacgtcttcg
     2761 agcaggagcc gctccggccg acccatccgc tgcggacggc gcccaacacc atcctgaccc
     2821 cgcacgtcgc ctggtacagc gaggaggcga cgcagacgct caggcgccgc gtcgcaggag
     2881 aggtggcccg ggtcgctcag ggcgagtggc cccaatcgct cgtcaacccc gaggtccgtg
     2941 cgaccgcccg gctgcgggcc ggagtccgga tgtcgggacc ttcctgagcg ctcccttcgg
     3001 cgcgccactg ggtatattaa agtatggtag caacgcaatc gcgacccgag agggtaagga
     3061 gcgcagctaa tggtccgggt gctcgcgatc gcattcgggg cggtgacggc gcttgcgctg
     3121 ctcgccagcc tgacccccgt ggggcaggtc atgctggggc tgagaccggc agcgcaggcc
     3181 acgtccgtca cggtgatcga ggataagccc gccgagattc ctggggcgtc gcccgcgtcc
     3241 cccacacaac ctccgggcgc gcgttcgccg cagggcggtc ccggggtggt ggggaacgtc
     3301 gccaacatcc tgctgaatct cccgcagatc cttgaccaga cccgcgtcgg gccggcccct
     3361 ttggagacga agcccgacgt ccatcaggag agcaaaggcg cgggggaata acacctcgtc
     3421 agccggaaca gacggctaga gaagcgccgg gggacgcccc cagtgggggc gtcccccgat
     3481 cgtttggcgc gggccgactg ccgcgcggcc tagatatgcc ggggcgcgac ctgatccggt
     3541 aggccggggc cgggcatctg gggggccatc ggtcctggag tcggaaatcc ggggatctgc
     3601 ggtccctcat acggctgcat atagtaaaac tccgggttcc ccccgcgggg catgggcccg
     3661 gggcgaagct gatagagctt gccctggtag aagtacagga tcctgtcgca gtcttgcccc
     3721 ccggtcccgg gcgtgggcgc tcctgggccc tggtcctggc ccgggttcgg cagcgggatg
     3781 aactccctgg ggttgaaggg catgccctgc gcgggattgg cagggagcag gatgacgtcg
     3841 tggccgctcg cggctgcgcc cgcgcgccat ccccaggcgg cggccttgcc gccgaccagg
     3901 aacaccccca ccagtcctcc gacgatgatg cccaggacga ggattcggac gccgcgcaca
     3961 gccatggtcc ccgctccctt ctccaaaggc ctcgtttccc ccatggtagc acacctcctg
     4021 cgcatccgca cgcgccccgg cggctaggcc gcgtgctaca atcagcactg aggtgatcgt
     4081 ggagtggcgc tgctgttcac ctttgcctgt gggcggtgcg gacggaagta cagcgtctac
     4141 tatccgaagg cgttcatcgc ctcggtgtac ggcaccatga ccccggcgca ggccgggcgc
     4201 gaggacgcgg acgaagtggc ctcaggcgcg atcgatgccg cacggcgtcg agccgaggcc
     4261 gcggggaata cctgggtcga tgtcagccag cagtccaagg tgacgtgcgt ctgcgggaag
     4321 aacctcaacc tgaacctggc cgatcacccg cgggtcccgc agcggaaggc cgccacgggg
     4381 gcccggcaga cgggcgtgat cgcgtttcct gagatgccga agaagcgcca cgacaagggg
     4441 ccggacggat ccggcgggcc tattccggtc gtgccgccgg agggcggccc accacgatca
     4501 ggcgtgtgat cttgatccct cggggcgtga cctcggcctc gtagtccacg atctccccgg
     4561 ggtccagccg gcgccgcccc gcccggcgca ggacgcgccg gtgcaggaag acatcgcggc
     4621 ccgccgcatc gaccacgaac ccgtagccca tccggctgtc gaaccacttc accacgcccc
     4681 gctccacgcg cgccgcccgc cttcgacttc tgggaccttc tgtttggcgt cgcgggagga
     4741 ggatcctgtc agctccccgc ccccccggag gctgggggcg gacgaggacc gcggggggga
     4801 gcccccggag caggttgtcg accgacgcgg tcacgtccgg gtggatcacg aacaggcccc
     4861 ggcgcacgat ctcgaccgcg cagtcgaaca cgggcgggcc ggtccgctcc agaatcgtgt
     4921 gccgcaccat gccccgtcgc tcgatctcct ccggcgacag cacgatcgtc tgcaggtcgc
     4981 ccaccaggca ggccagttcc gtgttgaaga gcacatcgcg cagcgaggct ccgtgcgcgg
     5041 tggcgatcaa ttgaatcccc cggcgggcaa tggtccgggc cacctgcgcg tcggccgcga
     5101 accccagctc gtccacgata atcgtttccg cccggtgatt gacgaccgcc tggagaatga
     5161 ccccggcctg ccgggctccc gcatccgccg gccggtcgtg gtcgaggagc ggaatctgga
     5221 ggcggcgggc gctcccgatc gccgggtggg gcacctgtcc gtctccgccg atctcgttgc
     5281 tggtgtccgc gatgacgacc ctgcggtgta gccggtctga caggatcgcc gccgcgctgc
     5341 gcagcaccgt ggtcttcccc gctcccggtc tgcccaccac caagacgttc cgccgggact
     5401 cgagcagctc ttgcaggggc tgcgccacct cgccgatcgt ccgcccgatc cgaaaggtga
     5461 agccgacgat ctcgtggtac cgatcccgga tgcacgcgat ccgatgcaag gtgcgatcga
     5521 tgccggttcg attatcctcc cggaatcgcg tgacccggcc gagcacgtat tgaaggtcct
     5581 cccggctgac ctcccgtgcc agcgtcaccg gctcgtcatg atcatggaag tggagtcgcg
     5641 gcggccgacc caggtccagt tcgatctcct cgaccgcatc cagatcgttc ccctcgaccg
     5701 acgcgacaat ccagcggggg accacggcca ggagggcggc gacctcgctg ccggccgtca
     5761 tcggcgagtt gggcccctag ggagcgctgg aggcggggag gcgatccaat ccggcaaaca
     5821 ggagctggaa gacggccggc agggtgccgc cggggctctg cctgtcctct ttcgcgcgga
     5881 acagtgagaa gatgacctcc acctggcgag gggccggcac agatgcgttg atcattccgg
     5941 attcggtggc gcccggaggg aggacggccg ggcggctgcg gtcggcgttc tcccgggaca
     6001 tcccaaacgg gaccagcacc ccgttcgccc tgatgagcac cccgtttggc cggaccgaga
     6061 ggtccgtggc gctcgtgttg gtgatccgat agcggatctt gatgccgccg ggcccgcgga
     6121 acaactggaa cactcccgcg gcctctccaa aggtttgccg cgcttcgagg tactccggcc
     6181 tttccggtcg tggattggct cggagatcag gggtcgacgc ctggggaccg gccggcgggg
     6241 tcgagggctg cgggagtgtg gcctgtacga gcggcgggga ggtgcgcgtg tttggcggag
     6301 gcgtggtcac cacgtacacg agatctgtgg tccgcggacc gggtccaatc gtgagatccc
     6361 agatcgacgt gaggtcggca aaccacacga tcatgttggt cgtcccggtc gccgcttgcg
     6421 gttgcagcac gaggtcgtac acgtcccctc cccgcttcac cgtcgtcgcg gtgaccagcc
     6481 ggggatcacc gatcgccacg gtatcgaccc ggcgatcggc ccgaaggacg gtggcgaacc
     6541 cgggctgcag gcgcagcgcc gtccgggtgg tcccgccggc aatgtacacg gtgagggtcg
     6601 cggggcctgc cccgctggcc gggctcccgc cccccgacgc aagggccacc aggaccacgc
     6661 agaacagccc ggtcccgagc gcgctcacgc ccgctctttc agaaactgct ccatgcgctc
     6721 cggcccccac tgggagacgt tgcggccttc catgagcagc ttgctcttga ggcggcggat
     6781 cttgtcctgg tcgatcggca ccgcaagggc ggcgagcgtg cttcggtcga cccccttgaa
     6841 gaagcgcaaa ccccctgagg ccggcggccg catccggtgg gcggacacct cttcgtcgac
     6901 ccagtcccgg atcacgtcct gcatcagccg cccctcgagg attagccgcg cccggaaccg
     6961 ccggtggagg tccctgggga cgtggatccc catcttggcg atcttggtgc gcatccccgt
     7021 cgtccgtccc ctaccgcgtg aggcgcgtgc gatccacatg gtccccccgg gccgcgatgg
     7081 atgtgtctgc ctactcatat agcatcatac cagtatggtt ggatggtcgc aacgagcgcc
     7141 ggagaaggtg cggcg
//
LOCUS       DASQGH010000593         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39526, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000593 DASQGH010000000
VERSION     DASQGH010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39526"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VEW91_11840"
     CDS             <1..1219
                     /locus_tag="VEW91_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM92312.1"
                     /translation="YYLARAGCEVAVVEEKDGLGLEATESNAGIIAPGHSFAWASPRA
                     PMQLVRSLLGAQTAIRVRLKPDLRLYAWGWKFLRECTNERARRNTLIKLRLCQYSQTV
                     MTELVRSEEISYQATTRGVLYVYRDPAELEAGAQKMALLSEHGQQQQNLDANAVAKLD
                     PVFEPVKHKIAGAIRDLTDSSGNARLFTETLARVCREKFGVTFQLRTRITGLHADGDR
                     VTGVVTDRGVLTADAYVLALGVNSPIVARTLGVRLAIYPAKGYSSTFPLKEIRLAPSI
                     PGVDEQWLVAWSQMGDQLRLTSTAEFAGYDWRWTPRDFRNILRLARDLFPDAADYERG
                     RYRACLRPMTPDGPPILGTGRHRNLFFNVGHGHLGWTMASGSARIVSDLMLNRKPDLD
                     LEGLKPRPRAGGI"
     gene            complement(1216..>1734)
                     /locus_tag="VEW91_11845"
     CDS             complement(1216..>1734)
                     /locus_tag="VEW91_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92313.1"
                     /translation="QADFHHFFGMTDEEREEARGWFALIELARCPTRAFESGPGGHLP
                     EVTVRIAQAPPLQRWTIEDVLNYAHEQLYEYVLKREGSTWQAQPAAYPRPRPAALPQA
                     QPQTPQPRLPPRPAASGRRNAGRDRKLSRPLKITFDREDDVPPPARTGPKKSGIQTFL
                     DTLAEAIKHLRR"
BASE COUNT          294 a          541 c          572 g          327 t
ORIGIN      
        1 gtactacctg gcccgggcag ggtgcgaggt ggccgtggtg gaggagaagg atgggcttgg
       61 tctagaggca accgagagca acgcgggcat catcgctccc gggcattcat tcgcctgggc
      121 ctccccgcgt gcgccgatgc aactcgttcg atcgcttctc ggagcgcaga ccgccattcg
      181 ggtcagactc aagccagatc tccggctcta tgcgtggggc tggaaattcc tccgagagtg
      241 caccaacgag cgagcgcgtc gcaacaccct gatcaagctg cgtctgtgcc agtacagcca
      301 gaccgttatg accgagctgg tgcgcagcga ggagattagc taccaggcga ccacccgggg
      361 tgtcctttac gtctaccgcg atcctgccga attggaggcg ggcgcgcaga agatggcact
      421 cctatcggag cacgggcaac agcagcaaaa tctcgacgcc aacgcggtgg cgaagctcga
      481 tcccgtcttc gagccggtga aacataagat cgcgggggcc atacgggatc tgacggattc
      541 gagcggcaat gccaggttgt tcaccgagac cttggcgcga gtctgccggg agaagttcgg
      601 cgtgacgttc caactcagga cgcgcatcac cggcctccac gccgatggcg atcgtgtcac
      661 cggcgtggtg actgaccggg gcgtgctgac cgcggacgcc tacgtccttg cgctcggagt
      721 caacagtccc attgtggcgc gcacgctggg cgtgcggctg gcgatctacc ccgccaaggg
      781 atactcttcg acgtttccct tgaaggagat ccggctcgct cccagtatcc ccggcgttga
      841 cgaacaatgg ctcgtggcat ggtcccaaat gggcgatcaa ctacggctta cttcaaccgc
      901 cgagttcgcc ggatacgact ggcgctggac acctcgggac ttcaggaaca tccttcggtt
      961 ggcccgcgac ctttttcccg acgctgccga ttatgagagg ggtcggtacc gggcctgtct
     1021 tcggccgatg accccggatg gtccgccgat cctgggaacc ggccggcacc gaaatctgtt
     1081 cttcaacgtc ggccacgggc acctgggctg gacaatggcc tccggttcgg cccgaatcgt
     1141 ctccgacttg atgttgaatc ggaagccaga tctcgatctg gagggtctca agccccggcc
     1201 gcgagccggg gggatctagc gccttaggtg cttgatggcc tccgcaagcg tgtccaagaa
     1261 cgtctggatg ccactcttct ttggccccgt cctcgcaggc ggcgggacgt catcctctcg
     1321 gtcgaaagtg atcttcagcg ggcgtgagag tttccggtcg cggcccgcgt tcctccgccc
     1381 agatgcagcc ggcctgggcg gtaggcgtgg ctgcggggtc tggggctggg cctgggggag
     1441 ggccgccggg cggggccgcg ggtacgccgc cggctgggcc tgccaggtcg agccttctcg
     1501 tttgagcaca tactcgtaaa gttgctcgtg ggcgtagttc aagacatctt cgatcgtcca
     1561 tcgctgcaac ggcggcgctt gggcgatgcg aaccgtgacc tccggtagat ggccgccggg
     1621 gcccgactcg aacgcccttg tggggcaccg ggcaagctca atcagggcga accatcccct
     1681 cgcctcttcg cgttcttcgt cggtcatccc gaagaagtgg tgaaagtcgg cttg
//
LOCUS       DASQGH010000594         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2541, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000594 DASQGH010000000
VERSION     DASQGH010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..362
                     /locus_tag="VEW91_11850"
                     /pseudo
     CDS             207..362
                     /locus_tag="VEW91_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            773..1528
                     /locus_tag="VEW91_11855"
     CDS             773..1528
                     /locus_tag="VEW91_11855"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92314.1"
                     /translation="MPGGATYPAIEGVSFTVGRGEFLAIVGPTGCGKSTILSLAAGLL
                     RPTLGRVLTFSALLDGLNRHAAYLFQQDVLLPWKTALDNVMLGPLLHHWPRSRAQDEA
                     RRWLARVGLAGFESRFPHQLSGGMRKRVALAQSLIVGPGILLMDEPFSALDVQTRELM
                     ENDLLALWQEDRKTIVFVTHDLEEAIALSDRVLVLAAGPATRVIGAHPVDLPRPRDVT
                     EVRLHPGFRHIYQMIWAQLRGEVLRAYAAQGRA"
     gene            1525..2298
                     /locus_tag="VEW91_11860"
     CDS             1525..2298
                     /locus_tag="VEW91_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92315.1"
                     /translation="MSNGRYRLLPYQAGFLVVLLAAWQVTTQRGWVDQFFFSYPSAIG
                     ARVVEWFVRGSIYRHLWTTALETVLAFLVGAGLGVAFGFAFARNPVLSGIFDPYLKIA
                     NALPRVVLAPIFTLWFGLGILSKVAFGVTLVFFIVFFNTYQGIREVDANLLNNTRMLG
                     ATERDLVRHVLLPSAMVWILSSLHTSVGLALVGAVVGEYLGSARGLGYVIAQAEGTFD
                     TTGVFAGMAVLAAFTLVVDLLVTLVERRLLAWKPPAAHA"
     gene            2365..3399
                     /locus_tag="VEW91_11865"
     CDS             2365..3399
                     /locus_tag="VEW91_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92316.1"
                     /translation="MRRGLMITLVVGALLVTLVAGGSAQVESPRITLAVGGQALYIYL
                     PLTLAQQLGYFKEAGVDVEIVDVAGGARALEALLGGSASVVCGFIDHTIEMQAQGKSI
                     KMFVLYDRYPGLALAVTKAGQARGIKGIADLKGGKIGVTAPGSSTYFFAQYLLAKNGV
                     PVDEASYIGIGTAATAVAAARRGVVDALVNVDPTITILTKSGDAQILADTRTTLGTLR
                     VFGGPYPAGGLYATPAFIERNPKTVQALTTASVKALHWIKFHSAEEIADKMPDRFFQS
                     DKSLYVASLKEDLEMFSLDGRIPLAGLQTIAKVLAAFDKTVVANPGRVNPAAAFTNEF
                     VDTAHTLLKF"
     gene            3416..>4215
                     /locus_tag="VEW91_11870"
     CDS             3416..>4215
                     /locus_tag="VEW91_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM92317.1"
                     /translation="MSATPRERFTALAQMPGTELPLAEGALLIAAEDYPALDIPRYLG
                     VLDHLAQEARARVPAGDDPLPAVGALCDFLFTTAGFRGNTRHYGDPKNSFLNEVLERR
                     LGIPISLALVYLEVGRRLGLPMYGVGMPGHFLVGCEARGGPLFVDAFNGTVLTEPGCG
                     HLFRQITGGASPFRKEYLAPTPARYILVRMLRNLKAIFVQDQDLERAAAAIERILLLA
                     PDSLEDVRDLGLTRFRQGSLAKARDLLEQYLRAVPRDAGDRAAVELSLA"
BASE COUNT          658 a         1446 c         1418 g          693 t
ORIGIN      
        1 aagcggcccg ctcccgttgg cgagaagaca cgcacacatt gccttaaacc cgaggacatc
       61 gccgatctct gcctctacct cgtctcgatg cggccggagg cgtgcattgc cgaggtcacg
      121 atcctgccca cggcgctgca ggcagtcggc aagccggcag tccagatgag gtaggataac
      181 acttcaccaa catcgggcac gatgaccgcc actgggtatg cggcaaccgt ggcatctcag
      241 agtggacatt gacaggaacc accggcgtcg gggttcgcat cgaggtgcgc ggctgcgagc
      301 actgggagtt tcgggatggc aaggttgtta ggaaagactc ctactggaag attgtcgagt
      361 aaagccatga cggcgagaac cgcctcagcg atcaggagaa gaacaggatc cgccactaag
      421 actcacgcag aatcctctca tttgcgcgca gccgcagccg cgcccgagtg gacagaggcc
      481 gcgcggcgat aatgcgctga gcgattgaga tcggctcttg gatgtgtatg gggccagtat
      541 agcctcgccg gatcggtttg aaccgccgct tactccaccg cacccctact tggggtgttt
      601 cggatctatc tgaacctgta cagggtagga gaggagtttg gccggcgctg agcgtacaac
      661 acggcactct gaacgcaatc gtgattcccg cggccccgcc cgcgcccccg tgacgtcgag
      721 cgagggcacc tctacggcgc tcgtcttcga cgccgtcagc cggcattttg cgatcccggg
      781 gggagccacg tacccggcga tcgagggcgt gtcgttcacc gtggggcgcg gggagttcct
      841 ggcgatcgtc gggccgaccg ggtgtgggaa gtccaccatc ctgagcctgg ccgccgggct
      901 gctccgcccc acgctgggcc gggtgctgac gttcagcgcc ctcctcgacg gcctcaaccg
      961 ccacgccgcc tacctgttcc agcaggacgt gctgctcccg tggaagacgg cgctcgacaa
     1021 cgtcatgctc gggccgctcc tgcaccactg gccgcggagc cgcgcgcagg acgaggcccg
     1081 ccggtggctg gcgcgcgtgg gactcgcggg gttcgagagc cggttccccc accagctctc
     1141 gggcggcatg cggaagcggg tggcgctcgc gcagagcctg atcgtcggac cggggatcct
     1201 cctgatggac gagccgttca gcgcgctcga cgtccagacc cgcgagctga tggagaacga
     1261 tcttctggcg ctgtggcagg aggaccggaa gaccatcgtg ttcgtcaccc acgacctgga
     1321 ggaggccatc gccctgagcg accgcgtgct ggtcctcgcc gccggccccg cgacccgggt
     1381 gatcggggcg cacccggtcg acctcccgcg cccgcgcgac gtcaccgagg tgcggctgca
     1441 ccccgggttt cgccacatct accaaatgat ctgggcgcaa ctccggggcg aggtgctgcg
     1501 cgcctacgcg gcccagggac gagcatgagc aacggccgct accgcctcct cccgtaccag
     1561 gcgggcttcc tggtggtgct cctggcggcc tggcaggtca cgacccagcg cggctgggtg
     1621 gaccagttct tcttcagcta cccttcggcg atcggggccc gggtcgtgga atggttcgtg
     1681 cggggatcaa tctaccggca cctgtggacc acggcgctgg agaccgtcct ggcgttcctg
     1741 gtcggcgccg ggctcggcgt cgcgttcggg tttgccttcg cgcggaaccc cgtcctctcg
     1801 gggatcttcg atccctatct caagatcgcc aatgccttgc cgcgcgtggt cctggcaccg
     1861 atcttcaccc tgtggttcgg gctcggcatc ctgtcgaagg tggcgttcgg ggtgaccctg
     1921 gtcttcttca tcgtgttctt caacacgtac cagggcatcc gggaggtgga tgccaacctc
     1981 ctcaacaaca cccgcatgct cggcgcgacc gagcgggatc tcgtccggca cgtcctgctc
     2041 ccctcggcga tggtctggat cctgagcagc ctccacacga gcgtggggct ggccctcgtc
     2101 ggcgccgtgg tcggcgaata cctcggctcc gcccgcgggc tcgggtacgt catcgcgcag
     2161 gccgaaggca ccttcgacac gaccggcgtg tttgccggga tggcggtgct ggccgccttc
     2221 accctcgtgg tggacctgct ggtcaccctg gtcgaacgcc ggctgctcgc ctggaagccg
     2281 ccggccgcgc acgcgtaacc ccgcgcggtg gaaggaggct tcccgctccc gccagtatct
     2341 tcctcgtaca gccggaggtg gaagatgcga agaggactca tgatcacgct ggtcgtgggc
     2401 gcgctgctgg tgaccctggt cgcaggcgga agcgcccagg tcgagagccc gcggatcacg
     2461 ctggcggtcg gcggtcaggc gctctacatc tacctcccgc tcaccctggc gcagcaactg
     2521 ggctacttca aggaggcggg cgtcgacgtc gagatcgtcg acgtcgcagg gggcgcacga
     2581 gccctcgagg cgctcctggg agggagcgcc agcgtcgtgt gcgggttcat cgatcacacg
     2641 atcgagatgc aggcccaggg caagagcatc aagatgttcg tcctgtacga tcgctacccg
     2701 gggctggcgc tggcggtgac gaaggccggc caggcgaggg gcatcaaggg gatcgccgac
     2761 ctcaaaggcg ggaagatcgg ggtgacggcg cccgggagca gcacgtactt cttcgcccag
     2821 tacctgctcg ccaagaacgg ggtcccggtc gatgaggcgt cgtacatcgg gatcggcacg
     2881 gccgccaccg cggtcgcggc cgcccggcgc ggcgtcgtgg acgcgctggt caacgtcgac
     2941 cccacgatca ccatcctgac caagagcggc gacgcccaga tcctggccga cacccggacc
     3001 accctcggca ccctccgcgt cttcggcggc ccctaccccg ccggcgggct gtatgccacc
     3061 ccggcgttca tcgaacgcaa cccgaagacg gtgcaggcgc tgacgacagc ctcggtcaaa
     3121 gcgctccact ggatcaagtt ccactccgcc gaggagatcg cggacaagat gccggaccgg
     3181 ttcttccagt cggacaagtc cctgtacgtg gcgtccctca aggaggacct ggagatgttc
     3241 tcgctggacg gccggatccc gctcgcgggc ctccagacga tcgcgaaggt gctcgcggcg
     3301 ttcgacaaga ccgttgtcgc gaaccccggc cgggtcaacc ccgccgccgc gttcacgaac
     3361 gagttcgtgg acaccgccca cacgctcttg aagttctaag cccggttggc gggccatgag
     3421 cgcaacgccg cgcgaacggt tcaccgcgct ggcgcagatg cccggcaccg agttgccgct
     3481 cgccgaaggc gcgctcttga tcgcggcgga ggactacccg gccctcgaca tcccccggta
     3541 cctgggcgtc ctggaccatc tggcccagga ggcccgcgcc cgcgtgccgg ccggagacga
     3601 tccgctgccg gcggtcgggg cgctctgcga cttcttgttc acgacggcgg ggttccgggg
     3661 caacacccgg cactacggcg accccaagaa cagctttctc aacgaggtgt tggagcgtcg
     3721 gctcggcatc cccatctccc ttgcgctggt ctacctggag gtcgggcggc ggctcggcct
     3781 gccgatgtac ggggtcggca tgcccgggca ttttctggtg ggctgcgagg ctcggggcgg
     3841 gcccctcttc gtcgacgcgt tcaacgggac ggtgctgacc gagcccgggt gcggacatct
     3901 gttccgccag atcaccgggg gcgcatcgcc gttccgaaaa gaatatctgg ccccgacccc
     3961 cgcgcgttac atccttgtcc gcatgctgcg gaacctgaag gccatcttcg tgcaagacca
     4021 ggacctggag cgcgcggcgg ccgcgatcga gcggattctg ctcctcgccc ccgactccct
     4081 ggaggacgtc cgggacctgg ggctgaccag attccgtcag gggagccttg cgaaggcccg
     4141 ggatctcctc gaacagtacc tccgggccgt gccccgggac gccggcgacc gggccgccgt
     4201 cgaactgtcc ctggc
//
LOCUS       DASQGH010000595         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_56152, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000595 DASQGH010000000
VERSION     DASQGH010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56152"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VEW91_11875"
     CDS             <1..285
                     /locus_tag="VEW91_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92318.1"
                     /translation="YRALGDDRRVRRLWEGVARELPGGGGCRLQILAEVAKARERAGD
                     LEGACATAEEALSAATVLQARGALHPPHLMDRLTRKVDRLGRLLQSSRSQ"
     gene            complement(337..744)
                     /locus_tag="VEW91_11880"
     CDS             complement(337..744)
                     /locus_tag="VEW91_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HYM92319.1"
                     /translation="MRVVADTNVLVSAIVFGGPPGRLVELAAEGHLQLILSPPLIEEL
                     RGVLRRKFGFSDTAAYQAEALLRRISIVVEPPRGVAIISEDPEDNRVLEAARAGDADV
                     IVSGDRHLLSLKRFGATAIRNPRELLNEIESSL"
     gene            complement(744..995)
                     /locus_tag="VEW91_11885"
     CDS             complement(744..995)
                     /locus_tag="VEW91_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HYM92320.1"
                     /translation="MKTRTMNISLPERLVEALDRRAEAEARSRSEVIRAAALSYLQWW
                     GEWRKLQAYGRSQAKRLGIRPQRLERLIAQVRTERPSHR"
     gene            1018..1191
                     /locus_tag="VEW91_11890"
     CDS             1018..1191
                     /locus_tag="VEW91_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92321.1"
                     /translation="MYDNVKGGATLEAPASSYAFEIGASGDPSVVPITNPVTVTLTVG
                     NNRGSATVTANII"
     gene            complement(1311..1808)
                     /locus_tag="VEW91_11895"
     CDS             complement(1311..1808)
                     /locus_tag="VEW91_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92322.1"
                     /translation="MSAWRTIHREERGLALPLAMLVVLILSALMFGLAETLTSEFSAA
                     LPASRDVRANYLAQAGIEHQIYLLKVNKNAAAIGLTNYPVTPGQEYWYSTTLQCLLSC
                     STNFETRRWQITGTGEIHQSGTSTVLQNRSIRAVVEIHYGGTGGSLFLFPTKVLVLRW
                     EEVYP"
     gene            complement(1805..>2370)
                     /locus_tag="VEW91_11900"
     CDS             complement(1805..>2370)
                     /locus_tag="VEW91_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92323.1"
                     /translation="TGYRLFPGDFNNNRLRIYYSTSWGSAGSVIGSLNAGVTTGQSYY
                     GRFYLVGSSLQAKVWQVGTAEPTSWQLSITDSSVSSGNHYGQVDATNVSMDHRHRTMI
                     IRPRVAVEPGVTLGAETAGTRSDTLAALAGPFRSLSVSCFDATNASIACSPATPVRAV
                     RVTLVVMDPTGAVPDITLTDEAFRQRP"
BASE COUNT          420 a          746 c          806 g          398 t
ORIGIN      
        1 taccgcgcgc tcggagacga ccggcgggtc cggaggctct gggagggggt ggcgcgggag
       61 ctacccgggg ggggcggctg tcgtctgcag attctcgcgg aggtggcgaa ggcccgggag
      121 cgggcaggcg acctggaagg cgcgtgtgcg accgcggagg aggcgctttc tgccgcgacg
      181 gtactccaag cgagaggggc gctccacccg ccgcacctca tggatcgcct cacgcgaaag
      241 gtggatcggt tgggtcggtt gttgcaaagc agccgctcgc aatgatccaa gactaatact
      301 tctgatctag atcgaacagg gacgtggctg tccatactac aaggaacttt cgatctcgtt
      361 gagcagttct cgcggattcc tgattgccgt ggcgccaaac cttttcaaac tgagcagatg
      421 gcgatcgcct gagacaataa cgtcggcgtc acccgcgcga gctgcctcca gcacgcggtt
      481 gtcctccggg tcttcgctga taatcgcgac ccctcgtggc ggctcgacca caatgctgat
      541 cctacgaagc agcgcttccg cctggtaggc cgcggtgtca gagaaaccga acttccgacg
      601 gaggacccct cgaagctcct cgatcagcgg cggtgacagg atcaactgca ggtggccttc
      661 tgcagcaagt tcgacgagcc ggccgggcgg accaccaaac acgatagcgg acacgaggac
      721 gttcgtgtcc gccaccacgc gcattagcga tgagagggtc gttcggtgcg gacctgcgcg
      781 atcagccgtt caagacgttg cggccgaatc cccagccgct tggcctggct gcgcccgtat
      841 gcctgcagct tgcgccattc cccccaccac tggaggtagg acaaagcggc cgctcggata
      901 acttcgcttc gcgaacgcgc ttcggcttcc gcacggcggt cgagcgcttc cacgaggcgc
      961 tccggcagtg aaatattcat cgtgcgcgtt ttcatctcaa ttatcaccat acaagccttg
     1021 tatgacaatg tcaagggagg ggcaacgctg gaggcccccg ccagtagcta cgcgttcgag
     1081 atcggcgcct cgggcgaccc gagcgtggtc cccatcacga acccggtgac ggtgaccctt
     1141 accgtgggca acaatcgcgg gagcgcaacg gtcacggcca acatcatcta ggtccagctg
     1201 ggttcaacgc gagccggcca tcgaaggatc gatcgcggtg ctggctcccg tctttattcc
     1261 aaagcacggc ctcgattggg aaggagaacc gcgcgccccg ccccggcgac ttaagggtag
     1321 acctcttccc agcgcaggac gagcaccttg gtggggaaaa ggaacaacga ccctccggtg
     1381 cccccgtagt ggatctccac cacggcccgg atgctgcggt tctgcaggac cgtcgaggtg
     1441 cccgattggt ggatctcgcc ggtgccggtg atctgccacc gccgcgtttc gaagttggtg
     1501 ctgcaggaga gcaggcactg gagggtcgtg ctgtaccagt attcctgccc cggggtcacg
     1561 ggatagttcg tcaggccgat cgcggcggcg ttcttgttca ccttgaggag gtagatctga
     1621 tgctcgatcc cggcctgggc caggtagttg gcccggacgt cgcgggaggc gggcagcgcg
     1681 gcgctgaact cggaggtcaa cgtctcggcc agcccaaaca tgagcgcgga gaggatgagc
     1741 accaccagca tcgccagcgg tagcgcgagg ccccgctcct cccggtggat cgtgcgccac
     1801 gcgctcatgg gcgctgccgg aaggcctcgt ccgtgagggt gatgtcgggg acggcgccgg
     1861 tcggatccat cacgacgagc gtgacccgga cggcgcgcac gggggtcgca ggggagcagg
     1921 cgatcgaggc gttcgtagca tcgaagcagg acacgctgag cgaccggaag gggcccgcaa
     1981 gcgcggcgag cgtgtccgac cgcgttccgg cggtctcggc cccgagagtc acgcccggct
     2041 cgaccgcgac ccgcggccgg atgatcatcg tgcggtggcg gtggtccatc gagacgttgg
     2101 tcgcgtccac ctggccatag tggttgccgc tcgacacgct ggagtccgta atcgagagct
     2161 gccatgacgt gggctccgcg gtgccgacct gccagacctt cgcctggagc gaggacccga
     2221 cgaggtagaa ccggccgtag tagctctggc cggtagtgac ccccgcgttc aggctgccga
     2281 tcaccgaccc ggcgctcccc cacgaggtcg agtagtagat gcgcaggcgg ttgttgttga
     2341 agtccccggg aaagaggcgg taccccgtgc
//
LOCUS       DASQGH010000596         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36613, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000596 DASQGH010000000
VERSION     DASQGH010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36613"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VEW91_11905"
     CDS             <1..162
                     /locus_tag="VEW91_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM92324.1"
                     /translation="EAAIQRRMAAVPLGRLGEAREVASVVAFLASDAASFVTGATLQV
                     DGGIVRSVL"
     gene            215..1093
                     /locus_tag="VEW91_11910"
     CDS             215..1093
                     /locus_tag="VEW91_11910"
                     /inference="COORDINATES: protein motif:HMM:NF005679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYM92325.1"
                     /translation="MSRAMRMIERISIAPGAEPTDVPVDITPGVEAYPDAEFVGGRHT
                     YGEALGIIMMHKYKARIPGDLGNAETFEFPVRYEVVAEASGTRHRRADPTLLEPFVRA
                     ALRLQEAGVRGITTSCGFLAFYQDAIAKRVEIPVLTSSLLLIPMVHRMAGRRGHVGVI
                     TVEGNHLDDRYFEAVGAEDVPIVLAGMEDQPEFRRAVIDDGPGVNPRRMAREMVHVAR
                     AMLARVPDIHAFVLECTQMPPYAYAIQQVTDRPVYDITTLSRLVYHSVVRSPFPIRHW
                     WVSRSPRTPQGATGPC"
     gene            1087..2229
                     /locus_tag="VEW91_11915"
     CDS             1087..2229
                     /locus_tag="VEW91_11915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYM92326.1"
                     /translation="MLKVVVAGAGIIGATCAYYAARAGHRVTVFDPGEIGAATTAVSG
                     SSVLAQTKTSPLLLDLTRRSQALYEELKRDLGVPYQVEGSLILFRTANEEAFVRDRCV
                     WLRAQGVRVDMLSGAEVRTQVPSVAARVRGAAYAPLDAEVAPREAAVAIAHGAAQLGA
                     VFSTGIPVTDLDVAGGSIRGVVTPAGSTACDEVVLAAGPWTGRIAAPHGIAVPIRPQK
                     GELLFTGPAQVPLRGRVLAATYLMSKFGTQSPDGFSAGLVVGREPDGSIKIGSTRECA
                     QFDTTPTARAREILLREAREYVPDVAALPIRRQTAGLRPYSLLKRPIIGRVPGVGGLI
                     LACGHGGDGIALAPITGWLVAQFIEGAATGLEGELALAEPTEGSAG"
     gene            2226..3335
                     /locus_tag="VEW91_11920"
     CDS             2226..3335
                     /locus_tag="VEW91_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYM92327.1"
                     /translation="MKITGLETVPYRIPNRETHRIATLTLSALDNVIVRLRTDEEIEG
                     IGEAVSEPKWNSTVREAHKEVLQRYLAPAVIGMDPFMITEIWQRMDAAVNGHYSAKAA
                     VDIALYDLMGKALGLPVWRYLGGGRREIPVEGPGFGIGFMEPRAAADFALTAVRRGCR
                     EIEIKGGHPAGWRRDLEVTRAVREACGPSVSLKVDITEAYTFKTALSALSRLADLGVE
                     WVEQPLPRHQLDDLARLRSAVPVGIMLEESVGHPADILRIAEIGAADAIHVKVPMLGG
                     ITMSREIEVICRAAGLGIQAGSSTPSGIGLAAVHQFAAAVPKLVRGCHGSPLARAVDD
                     IVVNPVDAYAATITCGDRPGLGIEVDWDKVERYRV"
     gene            3411..>4519
                     /locus_tag="VEW91_11925"
     CDS             3411..>4519
                     /locus_tag="VEW91_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HYM92328.1"
                     /translation="MQITETQAVHVRVPLETPYVISRGQMDAFDSVIVRLATEDGDVG
                     YGESVPLSVVGDPVVLAQLINGPIADILRGADPFDVERLVARAAEVVGPHIDALGGVD
                     LALWDLMGKYLREPVYRLLGGVCQELIQVDFTVGAQAPDRMAEVAQAMTREGFRGVVV
                     KLTAKSVPEDVARVRAVRRALPESCTVRVDCNGGYTRDDAVTFLKAIRELEVEFVEQP
                     VAGDDLEGMRRCREVGIAISADESLNTPGDALALVRGQACDVLNIKVPKVGGLLLAKR
                     IAAIGAAAGLPVVVGGRTTLELARYASRHFAASTMPAVGRAHEGPGPASQALTDDIVT
                     TRTTRAMVKETQGHVRIDRGAGLGADVVWAQVQRYA"
BASE COUNT          715 a         1442 c         1665 g          697 t
ORIGIN      
        1 gaggcggcga tccagcggcg catggcggcg gtaccgctgg gacggcttgg cgaggcccgg
       61 gaggtggcgt cggtcgtggc gtttctggcg tccgacgcgg cgtccttcgt gaccggggca
      121 acgcttcagg tggacggcgg gatcgtgagg agcgtgctct agcggcggcc gcggtgtgcc
      181 gacggttccg agaatccggg gaggtgactg tgctatgagc cgggccatga ggatgatcga
      241 gcgcatctcc atcgctcccg gggcggagcc cacggacgtg ccggtagaca tcacgccggg
      301 cgtggaggcg tatccggatg cggagttcgt cgggggacgg cacacctacg gcgaggcgtt
      361 gggcatcatc atgatgcaca agtacaaggc ccgcatcccc ggcgacttgg gcaatgccga
      421 gacctttgag ttccccgtgc gctacgaggt cgtggcggag gccagcggta cgaggcaccg
      481 ccgggctgac cccaccctgc tcgagccgtt cgtccgcgcg gcgctccgcc tgcaggaggc
      541 gggggtccgg gggatcacga cgagctgcgg ctttctggcc ttctatcaag acgccattgc
      601 caagcgggtc gagatcccgg tactcacgtc gagcctgctc ctgatcccca tggtccaccg
      661 gatggcggga cgccgtgggc acgtgggcgt catcaccgtc gagggcaacc acctggacga
      721 ccgctacttt gaggctgtag gtgccgagga cgtgccgatc gtcctcgccg ggatggagga
      781 ccagcccgag ttccgacgcg cggtcatcga cgacggaccg ggggtgaacc cccgccggat
      841 ggccagagag atggtccatg tggcccgtgc gatgctcgcc cgcgtcccgg acatccacgc
      901 gttcgtgctg gagtgcaccc agatgccccc atacgcgtac gcgatccagc aggtgaccga
      961 ccgtcccgtc tacgacatca cgacgttgtc ccggctcgtc taccactcgg tcgtgcgatc
     1021 gccgttcccc atcaggcact ggtgggtgag ccgcagcccg cgcacgccac aaggagccac
     1081 ggggccatgt tgaaggtcgt ggtggccggc gcggggatca tcggggccac ctgcgcatac
     1141 tatgcggcac gcgcaggtca tcgcgttacc gtcttcgatc ccggcgagat cggagcggca
     1201 acgaccgcgg tgtccgggtc ctcggtgctc gcccagacaa agaccagccc gctgctgctc
     1261 gacctgacgc gccggagcca ggcgttgtac gaagagctca agcgcgacct gggagtcccg
     1321 taccaggttg agggcagcct gatcctcttc cggacggcga acgaggaggc attcgtgcgg
     1381 gaccgttgcg tctggctgcg cgcgcagggc gtgcgggtcg acatgctgag tggcgccgag
     1441 gtgcgcacgc aggtgccgag cgtggcggca cgcgtccgcg gcgctgcata tgccccgctc
     1501 gacgcggagg tggcgccccg ggaggcggcg gtggcgatcg cccacggcgc cgcgcaactc
     1561 ggcgcggtgt tctcgacagg gatacccgtg accgatctgg atgtggccgg gggatccatc
     1621 cgcggcgtgg tcactcctgc aggcagtacc gcctgcgacg aggtggtgct ggcggcgggc
     1681 ccctggaccg gacggatcgc ggcgccccat ggcatcgcgg tgcctatccg gccccaaaag
     1741 ggagagctgc tgttcaccgg tccggcgcag gtgcctttgc ggggccgcgt gctcgccgcg
     1801 acgtatctca tgagcaaatt cggcacgcag tccccggacg ggttctccgc cgggctggtc
     1861 gtgggccggg aaccggacgg ctccatcaag atcgggtcca cccgggagtg tgcgcagttc
     1921 gatacaacgc cgaccgctcg cgcccgcgag atcctgctac gggaggctcg cgagtacgtc
     1981 ccggacgttg ccgcgctgcc gatccgacgg cagacggccg gcctgcgacc ctactctctg
     2041 ctcaagcggc cgatcatcgg ccgtgtgccc ggtgtcgggg gcctgatcct ggcgtgcggg
     2101 catggcgggg atggcatcgc gctcgccccg atcaccgggt ggctggtagc gcagttcatc
     2161 gaaggcgccg ccacgggcct ggagggcgag ctggcccttg ccgagccgac cgagggatcc
     2221 gcgggatgaa gatcaccggg ctggaaaccg tcccgtacag gattccgaac cgcgagactc
     2281 accggattgc gactttgacg ctgtccgcgc tggacaacgt gatcgtgcgc ctgcgcaccg
     2341 acgaggagat cgagggcata ggcgaagcgg tcagcgagcc caagtggaac tccacggtcc
     2401 gagaggccca caaggaggtc ctgcagcggt atcttgcccc ggctgtcatc ggcatggacc
     2461 cgttcatgat tacggagatc tggcagcgga tggacgcggc ggtaaacggg cactactcgg
     2521 cgaaggccgc cgtcgatatc gcgctctacg acctcatggg gaaggccctc ggcctgcccg
     2581 tgtggaggta tctcggcggc gggcggcggg agatccccgt ggaggggccc gggttcggca
     2641 tcgggttcat ggagccgcga gccgcggcgg actttgccct tacggcggtt cgccgaggat
     2701 gccgggagat cgagatcaag gggggacatc ccgccgggtg gcgacgggac ctcgaggtca
     2761 cacgcgctgt ccgggaggcc tgcggcccgt cggtcagcct gaaggtcgac ataacggagg
     2821 cgtacacctt caagacggcg ctctcggcgt tgtcacggct ggcggacctt ggcgtggagt
     2881 gggtggagca gcccttgccc cggcaccaac ttgacgacct cgcgcgtctt cgaagcgcgg
     2941 taccggtggg gatcatgctc gaggagagcg tcgggcaccc ggcggacatc ctgcgtatcg
     3001 cggagatcgg cgctgcggac gccattcacg tgaaggtacc catgctcggc ggcatcacga
     3061 tgagccgcga gatcgaggtg atctgccgag cagcaggatt ggggattcag gcgggaagct
     3121 ccacgccgtc gggcatcggc ctggcggcgg tgcaccagtt cgccgccgcc gtgccaaagc
     3181 tagtgcgcgg ctgtcacggc tcgccgctgg cgcgcgcagt cgacgacatc gtagtgaatc
     3241 cggtcgacgc gtacgctgcg accatcacgt gcggcgacag gccgggactc ggcatcgagg
     3301 tggattggga caaggtggag cggtaccgag tgtgacgggg cccgcaggga cggcaccggc
     3361 accgcccgcc gctcgcgccg acgcaaagct atctgaaggg ggaacgcctc gtgcagatca
     3421 ccgagacgca ggccgtgcac gtgcgggtac cgttggagac gccgtacgtc atctcccgcg
     3481 ggcagatgga cgcgtttgac agcgtcatcg tgcgcctggc caccgaagac ggcgacgttg
     3541 ggtacggcga gagcgtgccg ctctccgtcg tgggtgaccc ggtcgtcctg gcccagttga
     3601 tcaacgggcc gatcgctgac atccttcggg gcgccgaccc ctttgacgtc gagcggctcg
     3661 tggcgcgcgc cgcagaggtc gtgggtccgc acatcgacgc gcttgggggt gtggacctgg
     3721 cgctgtggga cctgatgggc aagtacctgc gtgagccggt ctaccgcctg ctcggtgggg
     3781 tatgccagga actgatccag gtcgacttca cggtcggcgc gcaggcaccg gaccgcatgg
     3841 ccgaggtggc gcaagcaatg acgcgagaag ggttccgcgg ggttgtcgtg aagctgaccg
     3901 cgaagtccgt gcccgaggat gttgcccggg tccgggcggt acgccgggca ctgcccgagt
     3961 catgcaccgt gcgggtggat tgcaacggcg gctacactcg agatgatgcc gtgacattcc
     4021 tgaaagcaat ccgcgaattg gaggtcgagt tcgtggagca gcccgttgcg ggcgacgacc
     4081 tggaaggtat gcgccggtgc cgggaggtgg gcatcgcgat cagcgccgac gaaagcctga
     4141 acacacccgg tgatgccctc gcgctcgtgc gcggccaggc ctgcgatgtg ctgaacatca
     4201 aggtcccaaa ggtagggggg ttattgctcg ccaagcgcat cgccgcaatc ggcgccgccg
     4261 cgggactccc cgtcgtcgtg ggcggccgca cgaccctgga gttggcacgg tacgcgagcc
     4321 gtcatttcgc agcctcgacg atgcccgcgg tgggccgggc acacgaagga ccggggccag
     4381 catcgcaggc cctcaccgat gacatcgtca ccacccggac gacacgcgcg atggtcaagg
     4441 agacgcaggg gcacgtccgc atcgaccgag gcgcgggcct cggcgccgac gtggtgtggg
     4501 cacaagtcca gcggtacgc
//
LOCUS       DASQGH010000597         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_16788, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000597 DASQGH010000000
VERSION     DASQGH010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16788"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2137
                     /locus_tag="VEW91_11930"
     CDS             <1..>2137
                     /locus_tag="VEW91_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYM92329.1"
                     /translation="EGTKSITVWGGQVRASQVRVYHAAPDRTRLEYLAAGTQPGRIVI
                     IKGRIQMEYVPARNQVIERPAPEADEELLNRAVLPEILHNYNVGFGGTEQVAGRETRV
                     INIQGKFPGRPSLRIWVDRERRLILRFERYRADGTLQETSAFINVRFDPVFSPGLFTV
                     SAPPGAQVQQQRNPPSMTVEEIAQRVGFTPQVPSYLPTGFQLRGGRVVSVRGQPVATF
                     VYSDGVSTLTLFETRGPQGPPPTGRPVRIGSVEGTVAARGLATLLHWNVGGISYILVG
                     ELPQDDLIRVAASVRAVSGIRPAWLSALAPIAVAEAAEPPPGGTAPLWPGVPPVPISP
                     YVTNDTRPIGPGVVAEEVRIWRALKTSGLSPFVVKVTVAGDGSSRLAWVWFVYGMDWA
                     GGAGAAVREVAESARALAITAFRADPQIAQVVLTGQYQVSGSFDGRRSDVTFTARLGR
                     GPLLAAPAALDSERALAQAGDVWYSPALVAGALAMHPARPHEPRRAADRPPAAPALFG
                     DRSVESAEHFDGSLLQRIVETKHRLDGILFGIESDGRLWRGNPHRREIALTFDDGPNP
                     VATPLLLAILRRFGARATFFVIGEHGVPYPYLIKQMVTDGHEVENHTFHHPKLTTVDA
                     PTIRKELAATTELIRQIAGPPRWFRPPGGDYSPEVVDAARGAGLRLAMGTVNSGDWAG
                     PPPKLLADRVLARAEPGAIILLHDGTLNTV"
BASE COUNT          299 a          756 c          769 g          313 t
ORIGIN      
        1 acgagggcac caagtccatc acggtctggg gcgggcaggt ccgggcctcg caggtgcgcg
       61 tctatcatgc cgccccggat cgaacccggc tcgagtacct cgccgccggc acccagcccg
      121 ggcggatcgt gatcatcaag ggccggatcc aaatggaata cgtgccggcg cgcaaccagg
      181 tgatcgagcg gccggccccg gaggcggacg aagagctcct gaaccgcgcg gtgctccccg
      241 agatcctgca caactacaac gtcgggttcg gaggcaccga gcaggtggcg ggccgggaga
      301 ccagggtcat caacatccag ggcaagttcc ccggccggcc gtcgctgcgg atctgggtgg
      361 accgggagag gcgcctgatc ctccggttcg agcgctaccg ggccgacggc acccttcagg
      421 agacctcggc gttcatcaac gtccggttcg atccggtgtt cagccctggc ctcttcacgg
      481 tgtcggcccc gcccggcgcg caggtgcagc agcagcgaaa cccgcccagc atgacggtgg
      541 aggaaatcgc gcagcgggtc ggcttcaccc cccaggtccc ctcgtatctg ccgaccggct
      601 tccaactgag ggggggccgg gtcgtcagcg tccggggcca gccggtggcc acgtttgtgt
      661 actccgatgg cgtctcgacc ctcacgctgt tcgagacccg cggaccgcaa ggcccgcccc
      721 ccacggggcg cccggtccgg atcggctcgg tcgaggggac ggtcgcggcc cgggggctcg
      781 cgaccctgct gcactggaac gttggaggga tctcctacat cttggtgggg gaactgccgc
      841 aggatgacct catccgggtc gccgcctccg tccgggcggt ctcggggatc cgcccggcgt
      901 ggctttcggc gcttgcgccc attgcggtcg ccgaggcggc ggagccgccc cccgggggga
      961 ccgccccgct gtggcccggg gtgcccccgg tgcccatctc gccgtacgtc acgaacgaca
     1021 cgcgcccgat cggcccgggc gtggtcgccg aagaagtgcg catctggagg gccctcaaga
     1081 cctccgggct gagcccgttc gtggtcaagg tcacggtcgc cggcgacggg tcgagccgcc
     1141 ttgcatgggt ctggttcgtc tacgggatgg actgggccgg aggggcgggg gcggccgtcc
     1201 gggaggtggc ggagagcgcc cgcgccctcg ccatcacggc gtttcgagcc gacccccaga
     1261 tcgcccaggt cgtcctgacc gggcagtatc aggtgagcgg ctccttcgat ggccggcgca
     1321 gcgacgtgac cttcaccgcc cggctgggcc gcggcccgtt gctggccgcg cctgcggccc
     1381 tggactcgga acgcgccctc gcgcaggccg gggacgtatg gtacagtccg gcgctggtgg
     1441 ccggcgcgct tgcgatgcac cccgcgcgtc cccacgagcc gcgccgcgcc gccgaccgcc
     1501 ccccggccgc tccagcgctg ttcggcgatc ggtccgtgga gtcggccgag cacttcgacg
     1561 ggagcctcct gcagcgcatc gtcgagacaa agcaccggct cgatggcatc ctgttcggga
     1621 tcgagagcga cggccggttg tggcgcggga acccccaccg gcgggagatc gctctgacct
     1681 tcgacgatgg ccccaacccc gtggcgaccc cgctgctgct cgcgattctc cggcggttcg
     1741 gcgcccgcgc gacgttcttt gtgatcgggg agcacggggt gccgtacccc tacctgatca
     1801 agcagatggt gaccgatggg cacgaggtgg agaatcacac ctttcatcat ccgaagctga
     1861 cgaccgtgga cgctccaacg attcggaagg aactcgcggc gacgacggaa ctgatccggc
     1921 agatcgccgg gccgccgcgg tggttccgtc cccccggcgg tgactacagc cccgaggttg
     1981 tcgacgcagc gcggggagcc gggctccgcc tggcgatggg gaccgtgaac tcgggggatt
     2041 gggccgggcc tccgccgaaa ctcctggcgg accgggtgct ggcccgcgcg gagccggggg
     2101 cgatcatcct cctgcacgat gggacgctca ataccgt
//
LOCUS       DASQGH010000598         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5358, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000598 DASQGH010000000
VERSION     DASQGH010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5358"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VEW91_11935"
     CDS             complement(<1..778)
                     /locus_tag="VEW91_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HYM92330.1"
                     /translation="MSKETNGPGPQTMRGVAGKILRVDLASRSITVDHPDEHFYRAYL
                     GGAGFVSYFLLKEVPRGIDALDSRNRLIFALGPLTGLPMPGATRSCVGAKSPLTNGYS
                     KSEAGGFFPMALKKAGYDAVIVTGRADRPVYLLMTDEQVEIRDASHLWGKTVLETQDA
                     IAAETGQKTIRTAAIGPAGENLVRFACIMNDVKGAAGRGGLGAVMGSKNLKAVAASGR
                     WTPKVANPDKIRELTLTMNQGFYDNPLFSKSLHDVGTGAKA"
     gene            complement(775..1014)
                     /locus_tag="VEW91_11940"
     CDS             complement(775..1014)
                     /locus_tag="VEW91_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYM92331.1"
                     /translation="MRVAIQAMGSVRTRSFTGQGGTVVELPEGSKVIDALRAVGIADD
                     ELWNASVDGQLVEAERVLRDGDVLLVFTPIAGGTA"
     gene            1689..4070
                     /locus_tag="VEW91_11945"
     CDS             1689..4070
                     /locus_tag="VEW91_11945"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HYM92332.1"
                     /translation="METPVPPETLAAFDGDELRARVFWEKYALRGQDGTPVEPTPDRM
                     WDRLAHELAAVEPEQGQREHWQREFRWLLGDFRFLPGGRIMHAVGQLPGWKAAPINCF
                     VLPLRDDSLPAIYDLCREMAITFSRGGGVGIDLSPLRPRGAVVHNAARVSTGAVSFME
                     TFSLVTGTIGQAGRRGALMITIPDDHPDVLEFCRIKRDRTSVRYANISVLVSDAFMRA
                     AETDADWRLHFENREAGVRVERVIRARELWDELVRGARDWAEPGCLFIDTARRRGTTE
                     YNGMRVLTTNPCAEEFLEPYGLCCLGSINLLRFVRQPFAELPPEENVDWTDLRRAVRA
                     GIRFLDNVLTYADPHFPLEAQRDAARRTRRVGLGVTALGDMLAALGLRYDADEAITFA
                     ERLMELIKLEAYRGSADLAEEKGPFPAFEAGPHLAQEFFQNFPEELRQRIRAVGLRNA
                     AVMTVPPVGSGSVLAGVTSGIEPIFARAYVRRSESLGQERFRVVHPLVALYFRSRGQA
                     LPDLGAADDAEGTLRGLLPTPFVTAHEIDPFQRVRMQAAVARHVDNAVSSTINLPQDV
                     SVETVEKIYRRAWESGCKGITVYREGAREGVLLTEREAPARHPETPGPSARRPEEPLP
                     AEARGPLVPRPERLAGPTYRIPTAFGTAFITVTELDDQPFEVFGRLGKGGTDAEAAAE
                     AIGRMASILLRLRSAVPRLERLGLIAEQLERIGGSRSAGFGQDRVRSIPDAFAIAIRK
                     YLADRTVPGAPPIAAARPTGTNLVGDFCPRCQQSALIEAGGCITCTVCGFKEC"
     gene            4409..>5631
                     /locus_tag="VEW91_11950"
     CDS             4409..>5631
                     /locus_tag="VEW91_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HYM92333.1"
                     /translation="MGEVLPRQVVTIDGNEAAASVAYRLNEVIAIYPITPASPMGEFA
                     DEWAAERRPNVWGTVPEVVEMQAEGGAAGAVHGALQAGALTTTFTASQGLLLMIPDMY
                     KIAGELTGFTMHVAARTVATHALSIFGDHSDVMGCRQTGFAMLASGSVQEAHDLACIA
                     HAAAFRTRVPFLHFFDGFRTSHEIAKIELLSDDDLRTMIPDALVEPHRRRALTPDHPV
                     IRGTAQNPDTFFQAREAANPFYLTCPERVQETMDQFARLTGRRYRLFDYSGHPEADRV
                     IVLIGSAAETVHETVDWLLRSGEKVGVVKVRLFRPFSVADFVAALPRTVRAIAVLDRT
                     KEPGAPGEPLYLDVVAALQEGQADGLAPFSVVPVVVGGRYGLSSKEFTPAMARAVFAE
                     LAAPRPRRHFTVGII"
BASE COUNT          894 a         1980 c         1851 g          906 t
ORIGIN      
        1 cggcctttgc accggtgcct acgtcgtgga gtgacttgga gaagagcggg ttgtcgtaga
       61 agccctgatt catcgtcaag gtgagttccc ggatcttgtc cggattcgcc accttagggg
      121 tccaacggcc gctggccgcg acggccttta ggttcttcga gcccatcacg gcgccgagcc
      181 ctccgcgacc ggccgcgcct ttcacgtcat tcataatgca ggcgaagcga acgaggttct
      241 cccctgcggg gccgatggcg gccgtgcgga tcgtcttctg gcccgtctcg gccgcgatgg
      301 cgtcttgagt ttccaggacc gtcttccccc acaagtgtga agcgtcacgg atctcgacct
      361 gctcgtctgt catcagaagg tagacaggcc ggtcggcccg gccggtgaca atgacggcat
      421 cgtagccggc cttcttgagg gccatcggaa agaagccccc cgcctcgctt ttcgaatagc
      481 cgttagtgag gggagacttc gccccgacgc aacttcgcgt agcgccgggc atgggcaacc
      541 ccgtcagagg cccgagtgcg aaaatcagcc tgttccgtga atccaatgcg tcgattccgc
      601 gggggacctc cttgagcaga aagtaggaca cgaagcccgc ccctccgagg taggcgcgat
      661 aaaagtgttc gtcagggtgg tccacggtga tgctccgaga ggcaagatca accctcagga
      721 tcttccctgc aactccgcgc atggtctgcg gccccggtcc gttagtctcc ttgctcacgc
      781 tgtcccgccg gcaatcggag taaagaccag cagcacgtcc ccgtccctaa ggacgcgctc
      841 cgcttccaca agttggccgt cgacgctggc gttccagagt tcgtcgtctg cgatacccac
      901 tgcacggagc gcatcgatca ccttgcttcc ctcaggcaat tcaaccacgg tcccgccttg
      961 gccggtgaac gagcgggtgc ggaccgaccc catagcttga atggcaaccc gcatctacag
     1021 tgttcttgta gccctcacgc gaagcatagg cgatcgtcga ccaggccgac aatccggcga
     1081 gcaccctatt tgggaccacc cgtgaccccc ggcgggccct cgtcaggctt cgcctccggc
     1141 gatcgcggct taccgcgtga gcggaagggc ctcctccagc acatcgtcat gtggatgtgc
     1201 gataggtcaa aggacaaatc cagataatgg tcgacgaact tccgggtctg cgcggggtcc
     1261 acccgctcgg ggagcatggt caccgctcga acaccccccc gccgatctca cccggagggc
     1321 cgaagaccgc gcaaactgac gacggcggcg gacgatctgg ccctcgaggc gcctctcggc
     1381 cacagcttac atgcggcctg ggccaagcat gaacgccgtc ggggggggcg cgcgatccgt
     1441 tctgatgatg tgagggtaca tcaatcgttc ccacttcaga ggccgagggc gtgtcgcgct
     1501 tgccactatc gatagatcgc ccccccgata ccgcttcgat agcaggcgcc ggcgcgtatc
     1561 cgggctcggg cgaggcgacc cccggcgggg acctgaagcc aagatccgcc gtccgcccga
     1621 tactcagcct ccgccccgcc gtctaaactc gtctcggcga gcccatgcct acccctgagg
     1681 acggttcggt ggagacgccg gtcccacccg agacgctggc cgcgtttgac ggcgacgaac
     1741 tgcgcgcccg cgtcttctgg gagaagtacg cactccgggg ccaggacggg acgccggtcg
     1801 agccgacccc ggaccggatg tgggaccggc tggcgcacga gttggccgcc gtggagcccg
     1861 aacagggaca acgggagcac tggcagcgcg agttccgctg gctcctcggc gacttccggt
     1921 tccttccggg tggccgaatc atgcacgcgg tcggccagct gccgggctgg aaggccgccc
     1981 cgatcaactg cttcgtgctg cccctccggg acgacagcct accggccatc tacgacctgt
     2041 gcagggagat ggccatcacg ttcagtcgcg gcggcggtgt cgggatcgac ctctcaccac
     2101 tccggccgcg gggcgccgtg gtgcacaacg ccgcccgggt ctccaccggc gccgtctcgt
     2161 tcatggagac gttctcgctg gtgaccggca ccatcgggca ggcgggccgg cgcggagccc
     2221 tcatgatcac catcccggac gaccacccgg acgtgctgga gttctgccgg atcaagcgcg
     2281 accggaccag cgtccgctat gccaacatca gcgtgctggt cagcgacgcg ttcatgcggg
     2341 ccgccgagac ggacgcggac tggcggctcc acttcgagaa ccgcgaggcg ggcgtccggg
     2401 tcgagcgcgt gatcagggcg cgggagctgt gggacgagct cgtcagagga gcccgcgact
     2461 gggcggagcc ggggtgcctg ttcatcgaca ccgcgcgtcg ccgcgggacg accgagtaca
     2521 acggcatgcg cgtgctcacc accaacccct gcgccgagga gtttctcgag ccctacgggc
     2581 tgtgctgctt gggctcgatc aacctcctgc gtttcgtcag gcaaccgttc gccgagctcc
     2641 ctcccgagga gaacgtggat tggaccgacc tgcgcagggc cgtccgcgcg ggcatccgct
     2701 tcctggacaa cgtcctcacg tacgccgacc cgcacttccc gctcgaggct caacgagacg
     2761 cggctcggcg cacccgccgg gtcggcctcg gcgtcacggc gctcggcgac atgctcgccg
     2821 cgctcggact caggtacgat gccgacgagg cgatcacgtt tgcggagcgc ctcatggaac
     2881 tgatcaagct ggaggcctac cggggaagcg ccgacctggc cgaggagaag gggccgtttc
     2941 cggctttcga ggccgggccg cacctggccc aggagttctt ccagaacttc cccgaggagt
     3001 tgcggcagag gatccgggcc gtgggcctcc ggaacgccgc cgtcatgacc gtcccgcccg
     3061 tcgggagcgg ctccgtgctc gccggcgtca ccagcggcat cgagccgatc ttcgcgcgcg
     3121 cctacgtgcg gcgcagcgag tccctgggcc aggagcgctt tcgggtcgtg caccctctcg
     3181 tggcgctcta cttccgttcc cgcggccagg cgctgcccga tctgggcgca gctgacgacg
     3241 cggaaggcac cctgcggggc ttgctgccga cgccgttcgt caccgcccac gagatcgatc
     3301 cgttccaacg cgtcaggatg caggcggccg tcgcgcggca cgtcgataac gccgtgtcaa
     3361 gcacgatcaa cctgccgcag gacgtctccg tcgagaccgt ggagaagatc taccgtcggg
     3421 cgtgggagtc cgggtgcaag gggatcacgg tctaccggga gggcgcacgg gagggggtgc
     3481 tcctcaccga acgagaggcc cccgcccggc atccggagac gccgggcccc tcggcgcgcc
     3541 gtccggagga gcccctcccg gccgaggcgc gagggcccct cgtgccccgg cccgagcgcc
     3601 tggccggacc gacctaccgg attcccaccg cgttcgggac ggcgttcatc acggtcaccg
     3661 agttggacga tcagccgttc gaggtgttcg gccgcctagg caagggaggg accgatgcgg
     3721 aggcggccgc cgaggcgatc ggccggatgg cgagcatcct cctgcgcctc cgcagcgccg
     3781 tgcctcgcct tgagcgtctg ggtctgatcg ccgagcagct cgagcgcatc ggcggctcgc
     3841 ggagcgccgg cttcggtcag gaccgcgtcc gcagcatccc cgacgcgttc gccatcgcca
     3901 tccgcaagta cctcgcggac cggactgtgc ccggagcgcc gccgatcgct gccgcccgtc
     3961 ccaccgggac gaatctggtc ggggatttct gtccgcgctg tcagcagtcg gcgctcatcg
     4021 aggccggtgg atgcatcacc tgcacggtgt gtggcttcaa ggagtgttga ggacacgcat
     4081 ggcaggagtc gcgtgggggg acacgggagc cgctcgagcg gttcggcgag atggcgtgta
     4141 gcgcgatgcc taccgcttcg ccagcatcgc ggccgacctc catgtcaccc aggtcgtgag
     4201 cccgcaatcc gcgtggcttc ggttacgaaa agcttgccat ccacaacaac aataggatat
     4261 ccgcggtact aaggctatcg gacgctcgcc cgattgaccc ttctctgacg gcgggatacc
     4321 ttcacgttga acgctcaggc tccggcctgc ccttgagagc cgacccaccg agccacgggt
     4381 ccgcccacgg ggactgaggt gagagcgcgt gggtgaggta ctgccacggc aggtggtgac
     4441 gatcgacggc aacgaagctg ccgcctccgt cgcctaccgc ctcaacgagg tcatcgccat
     4501 ctacccgatc acgcccgcat cccccatggg cgaattcgcc gacgagtggg cggcagagcg
     4561 ccggccgaac gtctggggga ccgttcccga ggtggtcgag atgcaggccg aggggggagc
     4621 cgccggcgcg gttcacggcg ccctacaggc cggcgctctg accaccacct tcacggcgtc
     4681 ccagggcctg ctgctgatga ttcccgacat gtacaagatc gccggcgaac tgaccgggtt
     4741 cacgatgcac gtcgcggccc ggacggtcgc tacccacgcg ctctccatct tcggcgacca
     4801 ctccgatgtc atgggctgcc ggcagaccgg cttcgccatg ctggcgtccg ggtccgtgca
     4861 ggaagcccac gacctcgcct gcatcgccca cgccgcggcg tttcgcactc gcgtgccatt
     4921 cctgcacttc ttcgacgggt tccgcacctc tcacgagatc gccaagattg agctcctcag
     4981 cgacgacgac ctccggacga tgatccccga cgccctcgtc gagccgcacc ggcggcgggc
     5041 gctcaccccg gatcaccccg ttatccgggg caccgcccag aacccggata ccttcttcca
     5101 agcccgcgag gccgcgaacc ccttctacct cacctgtccg gagcgcgtgc aggagacgat
     5161 ggatcagttc gcccggctga ccgggcggcg ataccgcctg ttcgactata gcggccaccc
     5221 cgaagcggac cgggtcatcg tgctcatcgg gtccgcggcc gaaacggtcc acgagaccgt
     5281 cgactggctc ctgcgctctg gagagaaggt cggggtggtg aaggtccgcc tcttccgccc
     5341 gttctcggtc gcggatttcg tagccgccct cccccgcacc gtccgcgcga tcgccgtgct
     5401 ggaccggacg aaggagccgg gcgcaccggg cgagccgctg tacctcgacg tcgtcgcggc
     5461 cctgcaagag ggacaggcgg acggcctggc acccttctcg gtggtcccgg tcgtcgtcgg
     5521 cgggcggtac ggcctctcct cgaaagagtt cacgccggcc atggccaggg ccgtgttcgc
     5581 cgaactggcg gcgccgcgcc ccaggcggca tttcaccgtt gggatcatcg a
//
LOCUS       DASQGH010000599         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28087, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000599 DASQGH010000000
VERSION     DASQGH010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28087"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="VEW91_11955"
     CDS             complement(<1..1940)
                     /locus_tag="VEW91_11955"
                     /inference="COORDINATES: protein motif:HMM:NF037381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92334.1"
                     /translation="MITPMSRVTVLGPRRRQREVVGAVQELGVLHVDHVRPASEDIAP
                     RELGEEDQAARTAQESVRTRAEGILALLPVIETPPQDTAPYAAQSAPALHERLAPVET
                     DAQTLTRRLLEAEEEQEILAAYGRALEVLTPLLGLLEGSRSLEAVGFVTEARSPEAFD
                     RLRSDLRQVAGGRVELVSRPIDEHRMGAVVAFARQDGEAVRGALTRAGVSELRLPASV
                     RGIPLGQAVPHLQARSRALPAEIEHLRRELMELSQRSRAEVAAIATVARDAGHRFELM
                     AQIPQSRYAFILYGWVPTRRVPDVRDGLRKRYGREVMIYDEPAPVHHAERVPVLLDNP
                     RWLRPFELFLAIFDPPKYGTFDPTLLFAIGMPLWVGLIIGDVGYGLFLLAITRWLAAR
                     AAGGRPWRAVVGGMDFGFTLSPTVLRSVVTVLSWMTAWTFLFGIVYGEFFGDLPERFI
                     HQFINPGWHPWFDRLEFITTYLYLSIGFGLAQVYLGLIMQMVKAVRHGERRELIEAIA
                     LASGGTTIFLWLATQVHVLPAWFFSPLMAASAVLFLLSLLFSFTLGSLMWVMEAVSTF
                     GAFISYARIFAVGIAALALAVVANTLGSRHPGGFVFLVMGILIGAVAHVMFFGLTIIG
                     HILQPARLFWVEFFSKFKYYQDT"
     gene            complement(1937..2290)
                     /locus_tag="VEW91_11960"
     CDS             complement(1937..2290)
                     /locus_tag="VEW91_11960"
                     /inference="COORDINATES: protein motif:HMM:NF028309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HYM92335.1"
                     /translation="MAHQHDAVQPSPDEQILHEIATKERELQEQLAQAQREAARSLEE
                     AQRAAGAILASAREQAQQEAAAASREASAESQAVTEQILSRARADADVVRKRAQERMS
                     RAVELVVRTVLGETA"
BASE COUNT          428 a          872 c          857 g          352 t
ORIGIN      
        1 gtatcctgat agtacttgaa cttggagaag aactcgaccc agaaaagtcg ggcgggctgc
       61 aggatgtgcc cgatgatggt cagcccgaag aacatcacgt gcgcgacggc gccgatcagg
      121 atccccatca cgagaaagac gaacccgccc gggtgccggc tgcccagggt gttcgccacg
      181 accgccagcg cgagggcggc gatgcccacg gcgaaaattc gcgcgtagga aatgaaggcg
      241 ccaaaggtcg acacggcctc catcacccac atcaggctcc ccagcgtgaa tgagaacagc
      301 agcgacagca agaacagcac ggcgctcgcc gccatcagcg ggctgaagaa ccaggcgggg
      361 agcacgtgca cctgcgtggc gagccagagg aagatggtcg tgcccccgct tgcgagcgcg
      421 atcgcctcga tcagctccct gcgctcgccg tgccggaccg ccttcaccat ctgcatgatg
      481 aggccgaggt agacctgcgc gaggccgaac ccgatcgaca ggtagaggta ggtggtgatg
      541 aactccaacc ggtcaaacca cggatgccat ccggggttga tgaactggtg gatgaacctc
      601 tccgggaggt cgccgaagaa ctctccgtac acgatcccga agagaaacgt ccaggccgtc
      661 atccagctca gcacggtgac cacggaccgg agcaccgtag gggagagcgt aaacccaaaa
      721 tccatccccc cgacaaccgc ccgccacggg cggccgccgg cggcccgggc cgccagccag
      781 cgggtgatcg ccagcaggaa caacccgtac cccacgtcgc cgatgatgag ccccacccag
      841 agcggcatcc cgatcgcgaa caggagggtc gggtcgaacg tcccgtactt gggtgggtcg
      901 aagatggcca ggaacagttc gaagggccgg agccaccgcg gattatccag gagcacgggg
      961 acccgctcgg cgtggtggac cggcgccggc tcgtcgtaga tcatcacctc gcggccgtag
     1021 cgcttccgca ggccatcgcg tacgtcgggg acgcggcggg tggggaccca tccgtacagg
     1081 atgaacgcgt accgggactg ggggatctgc gccatgagct cgaagcgatg tccggcatcc
     1141 ctggctaccg tcgcgatcgc cgcgacctcg gcgcgcgacc gctggctcag ctccatgagc
     1201 tcacggcgga ggtgttcgat ctccgcgggg agcgcacggg agcgagcctg taggtgtgga
     1261 accgcctgcc caagcgggat cccccggaca ctcgccggga gccggagttc gctcacgcct
     1321 gcgcgcgtga gggcgccccg gaccgcctcg ccatcctgcc gggcaaaggc gacgacggcg
     1381 cccatccggt gctcgtcgat cgggcggctc acgagctcca cccggccgcc cgccacctgg
     1441 cgcaggtcgc tccggaggcg gtcgaacgcc tccggggaac gggcttcggt cacgaatccg
     1501 accgcctcca ggctccggct cccctccagc agccccagca gcggggtgag cacctcgagt
     1561 gcccggccgt aggcggcgag gatctcctgt tcttcctccg cctcgaggag gcggcgggtc
     1621 agcgtctgcg catccgtctc gaccggggcg aggcgctcgt gcagggccgg tgcggactgg
     1681 gccgcgtacg gggcggtatc ctgcgggggc gtctcgatca ctgggagcag ggcgaggatc
     1741 ccttcggccc gcgtgcggac gctctcctgg gccgttcggg cggcctggtc ctcctcgccg
     1801 agctcgcggg gggcgatgtc ctcgctcgcg ggccgcacgt ggtcgacatg cagcacgccg
     1861 agttcctgga cggcccccac gacttcgcgc tgccgccggc ggggcccgag cacggtgacc
     1921 cggctcatcg gggtgatcat gccgtctctc cgagcaccgt gcggaccaca agctcgaccg
     1981 cccggctcat ccgctcctgg gcccgcttcc ggaccacgtc ggcatcggcg cgggcacggg
     2041 agaggatctg ctcggtgacg gcctgggact ctgccgaagc ctcccgggac gccgcggcgg
     2101 cctcctgctg agcctgctcg cgtgcgcttg caaggatcgc gccggccgcg cgttgggctt
     2161 cctcaagact ccgcgccgcc tcgcgctgcg cctgcgccag ctgctcctgc agttcccgtt
     2221 ccttggtcgc gatctcgtgg aggatctgtt cgtcgggcga gggctgaacg gcgtcgtgct
     2281 gatgcgccat ggactctcct gggcaaaaaa aagcgtccgc gatccggacc cgtaccggga
     2341 cgcggcccgc ggacgttcgg gccacaagcc cggaccatgg taacaagcac ccaatttact
     2401 gtcaagggtc gcggcccgcc aacctcccga acacggcgat ccgccgtcag aacctcccga
     2461 tggtggttgc gatctccacc aggcgaagga agggctgggg aaagatgcc
//
LOCUS       DASQGH010000600         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_42279, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000600 DASQGH010000000
VERSION     DASQGH010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42279"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VEW91_11965"
     CDS             <1..463
                     /locus_tag="VEW91_11965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887346.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HYM92336.1"
                     /translation="DLTGYITEGQLVLSRSLHRLGVYPPINPLPSLSRLMNNGIGKGR
                     TREDHRQVADQLYSAYAQGLDLRRLVAIIGEEALTENDRLYLRFADAFEKEFIAQGSA
                     DRSIQDALTLGWKLLSMFPKGALTRISRDHVDKYYFSEQMERLYKPGERPG"
     gene            536..1180
                     /locus_tag="VEW91_11970"
     CDS             536..1180
                     /locus_tag="VEW91_11970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HYM92337.1"
                     /translation="MAETISPTRMNLLLRQNQVKLAQQGVDLLKRKRDALVADFFNIV
                     RRALAARERLTKAAEDAYTLLSLAKAVEGREVLESAALADRRHLEVEIETKNIWGTRI
                     PTITTNEVRRTILARGQDPVAITARTVESADHFEDVVGAVLEVASTEVTLRKIGEEIK
                     KTTRRVNALEQVVIPRVRGEIRYIRDVLEQRAREDVFRLKRIKKKLEAKATTPE"
     gene            1225..>1731
                     /locus_tag="VEW91_11975"
     CDS             1225..>1731
                     /locus_tag="VEW91_11975"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HYM92338.1"
                     /translation="MDLWRQTVAAILDAPGVVVMLGPVDVGKTTLATAIASAAVRTRH
                     RAAVVDADTGQSDIGPPTTVAVATPRRPAKRMDEWRATVAFFVGDTSPQHVYHYLVEG
                     AVRSVEWARARGIQVVVVDTTGWVEGDAAVAAKVHKIRRIAPRHVVALQRHGEVEPIL
                     DRLPRGITV"
BASE COUNT          308 a          554 c          632 g          237 t
ORIGIN      
        1 ggacctgacc ggctacatca ccgaaggcca gttggtgctg agccggtcgc tgcaccgcct
       61 gggcgtctac ccgccgatca acccgctccc cagcctgtcg cgcttgatga acaacgggat
      121 cgggaagggc cgcacccggg aagatcaccg gcaggtggcc gaccagctct actcggccta
      181 cgcgcagggg ttggacctca ggcgcctcgt cgcgatcatc ggcgaggagg cgctgaccga
      241 gaacgaccgc ctctacctcc gcttcgcgga tgcgtttgaa aaggagttca tcgcgcaggg
      301 ctccgccgat cgctcgatcc aggacgccct gaccctcggg tggaagctcc tctcgatgtt
      361 ccccaagggc gcgctcaccc gcatcagccg cgaccacgtg gacaagtatt acttcagcga
      421 gcaaatggag cgattgtaca agcccgggga gagacccggc tagcggcggg gcgcgcgcgg
      481 cgcagcctgg ggatgatggg gcacacgggg gcaggaccga gggagggtgg cggggatggc
      541 tgagacgatc agccccaccc gaatgaacct cctgctgcgc cagaaccagg tcaagctggc
      601 gcagcagggc gtggacctgc tgaagcgcaa gcgggatgcg ctggtggccg atttcttcaa
      661 tatcgtgcgg cgggcgctcg ccgcgcggga acggctgacg aaggcggccg aggacgcgta
      721 cacgctgctc agcctggcca aggcggtgga ggggcgggaa gtgctcgaat ccgcggcgct
      781 ggccgacagg cgccacctcg aggtcgagat cgaaaccaag aacatctggg gaacccgcat
      841 ccccacgatc acgaccaacg aggtgcggcg gacgatcctc gcgcgcggcc aggaccccgt
      901 ggccatcacc gcccgcaccg tcgaaagcgc cgaccatttc gaggacgtgg tcggtgccgt
      961 cctggaagtt gccagcaccg aggtgaccct ccggaagatc ggcgaggaga tcaagaagac
     1021 cacccggcgc gtaaacgcgc tcgagcaggt ggtgatcccg cgcgtccggg gtgagatccg
     1081 gtacatcagg gatgtgctgg agcagcgggc gcgggaggac gtcttccgcc taaagcggat
     1141 caagaagaag ctggaagcga aggcgacgac tcccgaatag ggtccgcgcc tgttccgtgc
     1201 gccccgcggg ggcggagacg aaacgtggac ctgtggcggc agacggtggc ggcgatcctg
     1261 gacgcgcccg gggtcgtcgt catgctcggt cctgtggatg tgggcaagac gaccctcgcg
     1321 accgcgatcg ccagcgccgc cgtacgaacc cggcaccgcg cggcggtggt ggatgccgac
     1381 accgggcaat ccgacatcgg gccccccacg acggtggccg tggccacgcc gcggcgcccc
     1441 gcgaagcgga tggacgagtg gcgcgcgacc gtggccttct tcgtcgggga tacgtccccc
     1501 cagcatgtgt accactacct ggtcgagggc gcggtccggt cggttgagtg ggctcgggcc
     1561 cgggggatcc aggtggtcgt tgtggacacc acggggtggg tcgagggtga tgctgccgtc
     1621 gcggcgaagg tgcataagat ccgccgaatc gctccgcggc acgtggtggc gctccagcga
     1681 cacggggaag tggagccgat cctggaccga ctgccgcgcg ggatcaccgt g
//
LOCUS       DASQGH010000601         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11013, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000601 DASQGH010000000
VERSION     DASQGH010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11013"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..642)
                     /locus_tag="VEW91_11980"
     CDS             complement(115..642)
                     /locus_tag="VEW91_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92339.1"
                     /translation="MALLLAALATLVSVAPAQTAAGPVAVVGEFTNMRFTQEHAYGYS
                     VQIWRQEDTLFGLFMASEGLQGDTPTGLLDDIEFDPQTGALTFTTRLTIGVAYLGDTP
                     VPSRDLFEFRGMLQRLTLIGTLTRWDRLHPAATPTTERVRLQKQPSTLMIQPASYGAW
                     KQWADEILGRRGPKW"
     gene            complement(681..>2073)
                     /gene="lon"
                     /locus_tag="VEW91_11985"
     CDS             complement(681..>2073)
                     /gene="lon"
                     /locus_tag="VEW91_11985"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HYM92340.1"
                     /translation="VVEYLAVRKLAPESKAPILCFVGPPGTGKTSLGKSIARALGRKF
                     VRVSLGGVRDEAEIRGHRRTYVGALPGRIIQGMRTAGSKNPVFMLDEIDKLGIDFRGD
                     PSAALLEALDPEQNNSFSDHYMELPFDLREVMFITTANILDTVPPALRDRLEVIRFSG
                     YIEDEKHHIATQFLIPKQLKENGLRPEYIGFTDESLRLIIRQYTREAGVRNLEREIAS
                     ICRKVAREVAEGEATSVKVTIQNVRKFLGPTKFHYGTAGKIDEVGAATGLVFTEQGGD
                     IITVEATLVRGDGKLTLTGQLGDVMKESAQAALSYIRARALALGADDSFTARYDVHVH
                     VPAGAVPKDGPSAGITMAVALASALTGRYVRKDVALTGEITLRGNVLPIGGLKEKVIA
                     AHRAGIKTVIVPKENEKDLVEIPANVKKKLRFVFVEHMDEVLKEALGPGLGLPVLTHA
                     PSDVRRSQPSIQA"
BASE COUNT          327 a          706 c          658 g          382 t
ORIGIN      
        1 gccactgatg ctcatggggc cagtatatcg gcccttcaca tgcagccgaa acccggcttt
       61 ccgtcgcggc actcaccgag gtgtagtcgg atctatctga acctgcacag gcgcctacca
      121 cttcggtcca cgccgcccca ggatctcatc agcccactgc ttccatgcgc cgtagctcgc
      181 cggctgaatc atcagcgtac tcggttgctt ttgcaaccgg acccgctcgg tcgtgggcgt
      241 ggccgcggga tggagcctat cccacctcgt cagtgtcccg atgagcgtga gccgttgcag
      301 catgcctcga aactcgaaga gatcacggga cggcaccggc gtgtctccaa gatacgcgac
      361 tccgattgtc agcctggttg tgaaagtcag cgcccccgtt tggggatcaa actcgatgtc
      421 gtcgaggagc ccggtcggcg tgtccccttg gagcccttct gacgccatga agagcccaaa
      481 gagcgtatcc tcttggcgcc agatctggac agagtatcca tacgcgtgct cctgggtgaa
      541 ccgcatgttc gtgaactcgc ctacgacggc aacaggcccg gccgcggtct gcgcgggggc
      601 aactgatacg agcgtggcga gcgcggcgag gagcaaggcc ataccccgcg atgcctcctc
      661 gcggcagccg gccgtcgagg ctacgcctgg attgacggct gcgagcgtcg cacgtcgctc
      721 ggggcgtggg tgagcaccgg cagcccgagc cccggcccga gggcctcctt gagcacctcg
      781 tccatgtgct ccacgaagac gaaccgcagc ttcttcttga cgttggcggg gatctccacc
      841 aggtcctttt cgttctcctt ggggacgatc accgtcttga tgcccgctcg gtgggcagcg
      901 atgaccttct ctttgagccc gccgatggga agcacgttcc ccctgagggt gatctccccg
      961 gtcagcgcca cgtctttccg cacgtaccgg ccggtgaggg cggaggcgag ggcgaccgcc
     1021 atcgtgatcc ccgccgaagg cccgtccttg ggcaccgcgc ccgcgggcac gtgcacgtgc
     1081 acgtcgtacc gcgccgtgaa ggagtcgtcg gctccgagcg cgagcgcgcg ggcccgaatg
     1141 taactgaggg cggcctgcgc ggattccttc atcacgtccc cgagttgtcc cgtgagggtc
     1201 agcttgccgt cgccccgcac cagggtcgcc tcgacggtaa tgatgtcgcc cccctgctcg
     1261 gtgaacacga gccccgtggc ggcgcccacc tcgtcgatct ttccggcggt gccgtaatgg
     1321 aacttcgtgg gcccgaggaa cttacgcacg ttttggatcg tcaccttgac cgaggtcgct
     1381 tccccctcgg cgacctcgcg cgccaccttg cggcagatcg acgcaatctc ccgctcgagg
     1441 ttgcgcacgc cggcctcgcg cgtgtactgc cggatgatga gccgcaggct ctcgtcggtg
     1501 aacccgatgt actcgggccg gagcccgttc tccttcaact gtttggggat gaggaactgg
     1561 gtcgcgatgt ggtgcttctc atcctcgatg tacccggaga accggatcac ctcaagccga
     1621 tcgcggagcg ccggcggcac cgtgtccaga atgttggccg tcgtaatgaa catgacctcg
     1681 cgcaggtcaa acggcaactc catgtagtgg tcgctgaacg agttgttctg ctcggggtcc
     1741 agcgcctcga gcaaggcggc cgaggggtcg ccgcggaaat cgatccccag cttgtcgatc
     1801 tcgtccagca tgaacacggg gttcttgctg ccggccgtgc gcatgccctg gatgatccgt
     1861 ccgggcagcg cccccacgta cgtacggcga tgcccgcgga tctcggcttc gtcacggacc
     1921 ccgcccaggg agacgcggac gaactttcgc cccagcgccc gtgcgatcga tttgccgagg
     1981 ctggtcttcc cggtccccgg cgggcccacg aagcagagga tcggggcctt ggactcgggc
     2041 gccagcttgc gcaccgcgag gtactccacg acc
//
LOCUS       DASQGH010000602         8875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28090, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000602 DASQGH010000000
VERSION     DASQGH010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28090"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VEW91_11990"
     CDS             complement(<1..232)
                     /locus_tag="VEW91_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92341.1"
                     /translation="MNGRSIPRSGSAISDAHIERWKQSFTDEELIELGTFMAFADGFG
                     KLVEMLGRPGGRPRGLRRRAGLAPPRIQHVGYR"
     gene            complement(229..1617)
                     /locus_tag="VEW91_11995"
     CDS             complement(229..1617)
                     /locus_tag="VEW91_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HYM92342.1"
                     /translation="MRAVIDLAIRGGTVVTSRGRFSADIHVHDGRIVALGALDAASAQ
                     VVDAGGLCAIPGVVDTHVHFMDPGETEREDFITGSAAAAVGGTTTVIEHTHAAPVLDA
                     EGLRRKVAHLGRRSVVDFGLAAHVWPDRLSQIPELWGAGAAYLKVFTCETHGVPAMLA
                     GSLLQCFRAAAACDGLLVVHCEDDAITRERERTLREAGRTDFGVVPEWRSREAEEVAA
                     AGTALLARLAGPRVVIAHTSHPEAVELIARERARGGRLWVESCPQYFFLREDEVVRHG
                     PFRKFTPPARLRSAEDAARMWGLLADGAITHLATDHAPATRQQKERGNIWQAPFGLPG
                     VETTLTLMLHAVHQGWVTLERVVDALCERPARLYGFYPRKGSLWPGADADVTLVDPGR
                     SRVIQDDQVVSKAGWTPYAGMAVVGGPVMTFCRGRLVARDGRVHEDPGWGRWLPGAGR
                     RLEDGHQDSPGR"
     gene            complement(1614..2651)
                     /locus_tag="VEW91_12000"
     CDS             complement(1614..2651)
                     /locus_tag="VEW91_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYM92343.1"
                     /translation="MKAMVVERYGAPLKMVDLPMPAPGPRQVLVRVIGCGVCRSDLKV
                     VAGAMPFSSTLPLPHVPGHEISGEIAALGPDASGRIGDRVVVYHYWACRACAHCLAGE
                     ENLCANLRGWAGFGTPGGFQEYLAVPDDCVLPLPANIPPEHGGPLTCALGTAYQAVVS
                     RGGVRAGEAMVVLGAGGVGLQAVQIGRAAGAVVLAVDLHAHRLEAARAAGAHDAFPAG
                     AGAGERVRAATAGRGADVVIDTVGHHGSLATAGGLVRTGGRIVVVGYATSPEEYPVLP
                     TDRVVLGQLSVLGTRYTTRLGLQRALDLVARGLVRPVISGTFPLEQANEALAAVREDR
                     ATGRVVVRVAG"
     gene            complement(2683..3714)
                     /locus_tag="VEW91_12005"
     CDS             complement(2683..3714)
                     /locus_tag="VEW91_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYM92344.1"
                     /translation="MKAQVIHSWGGPLTLVEIPRPTPQAGEVLVAVRACGVGLTVLNY
                     MNGQMARPDNLPRVPGHEITGVVAEIGPGVATVRPGDRVLVFFYLTCGRCEFCRAGRD
                     SLCRDHGGYVGVHRDGGYAAFVALPEANVLPLPEGVPFVEGTAIPDAIATPYHVCATR
                     ARVRVGDRALVIGAGGGVGIHMLQMARLFGAHVVAVDVNDAKLARCRELGATHAVNFR
                     ASDAEAQARAALDGGATVAVDLVGNEHTLAWAFGLLGRGGRMVTLTTFADVGFRVLPR
                     RLVVDELKILGSRYCSRAEVLAAARLVRDGRIRPVVSQTVALSEVESLHAMLREGRLF
                     GRGAVVFEE"
     gene            complement(3842..5020)
                     /locus_tag="VEW91_12010"
     CDS             complement(3842..5020)
                     /locus_tag="VEW91_12010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYM92345.1"
                     /translation="MHPETVRRLLEGVREFVAQAVVPVAAELDRRPHPEDCFSWEIVE
                     QASRAGIRTLTLGTEFGGAAADALTTAMVIEELARGDLGVSVIFAQTLKIAQTLQRLC
                     TSAQAGRFLPSFAKDPRGLLAIGITEPTTSSDYIIPFDPPDVQFHTTAVQRDGAWVLN
                     GMKHFISNGNRAMLYLVFAQTDPSASLVEGSTCFLVPRATPGFSIGRVHDKMGERLVN
                     NAELIFRDCRLADDQILGRVHQGFAILRQFFPASNAYAAASVLGVADAAYERALLWTR
                     DRIQGGRRLIDHDTTAQDLAEMRMLIDAARAYTHAAAESADRPEPMDPTLGALPKVFA
                     SRVAWWVVTRTLELHGGHGYMRDAGIEKLVRDAAAFLHSDGANRSLLLKAARVIRGAS
                     "
     gene            complement(5060..5875)
                     /locus_tag="VEW91_12015"
     CDS             complement(5060..5875)
                     /locus_tag="VEW91_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYM92346.1"
                     /translation="MVLPGGARVAIWTIVNVEEWDISRTLPRTVLPLPGGGSRVPDIT
                     NYGWYDYGLRVGFWRLRDVLARHRVRAAMAVNGSICTSYPRLVEAALESKWEIVGHGF
                     IQRPLPLEPDERAAIRRTVASIRRATGAAPRGWLSPGLAETWDTLDLLAEEGIEYVAD
                     WANDDQPYEMRVKRGRLVSVPYSLEINDIPMFLAQHHAAEELFRRTRDQFDTLFEEGK
                     TNARVLPIAVHPYITGVPHRIKYFDMIYAYLRAKSGVLFWTGAEILDWFKRAC"
     gene            complement(5970..6689)
                     /locus_tag="VEW91_12020"
     CDS             complement(5970..6689)
                     /locus_tag="VEW91_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HYM92347.1"
                     /translation="MAEILDRQEFVQALRSQLTVDPRRTAVLAVDVHRGHLDPEIGTM
                     PVSPDDAARVRAAVARLLGGARGAGVPVIYVLLTYRRTSPPGLESMGNPFWRAVEAVK
                     QSLTPGRRSTILHHNQEGAPQTEVPPELAPQPGDFVIRSKKRLSAFYGTDLDVLLRSL
                     GTETVVICGINTNTCVLCTAFDAFNRDLRVIVASDGVASMYGADLHVFGLENLKRCLG
                     WVLTTDEILVHLGTIPAHTAR"
     gene            complement(6739..7977)
                     /locus_tag="VEW91_12025"
     CDS             complement(6739..7977)
                     /locus_tag="VEW91_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92348.1"
                     /translation="MMRQYLHVFVACAVVLALLVPGGPVIAQQGLIKIGLIVPFTGVF
                     APNGRDMANGLAMALNQVGNKVAGREIQVLTEDEGAPGQALTKARKLVEFDRVDILMG
                     PLRADSGLALRDYIIEQKIPAIYPIVSADDLSQRLRTPWIIRTGWTSSQPNHPFGEYA
                     VKVLKYKRIATIAYDFAFGWENVEGFQDTFEQNGGRVVLHLWPPIGAPDYSPYLGRIP
                     RDVDAVYAAFSGGDALRFLQQYRDFGLEGRIPLIGNGTFTDEHILFQERDLPKGVLTA
                     LHYSAALATQANRDFVLAYVRAYSRVPSYYSEGAYTGALFFIKGLEAVHGRIADREAF
                     VAAVRGVVLPDAPRGPVRLDQWGNPIQNEYIRRVDIVNGQPQNTVIFTYPNVSQFWTA
                     NPDEYLKKPVYTRDLPPAHP"
     gene            complement(8011..8727)
                     /locus_tag="VEW91_12030"
     CDS             complement(8011..8727)
                     /locus_tag="VEW91_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92349.1"
                     /translation="MLRVDDIHTYYGDSYVLQGVSLEVRPGTVAALLGRNGMGKTTLI
                     RSIIGFARPRRGQVLFEGREITRLPSHEIARLGIGLVPQGRRIFPSLTVIENLTVAAR
                     NGGPVHDGPTGGPWTMERVLGLFPRLGERTGHRGSMLSGGEQQMLAIARALMTNPRLL
                     LMDEPAEGLAPLLVRDLGASIGRLKGAGLSILLVEQNLPMALAIADEIYVLSKGRVVY
                     HGTPAEMSADATVKHRYLGV"
BASE COUNT         1334 a         3261 c         3007 g         1273 t
ORIGIN      
        1 ctcgatatcc aacgtgttgt atgcgcggag gtgcgagtcc tgcccggcgt cttagacctc
       61 gtgggcgccc acccggccta cccagcatct cgaccagctt gccgaacccg tcggcgaacg
      121 ccatgaaggt ccccagctcg atcagctcct catcggtgaa ggactgcttc cagcgctcga
      181 tgtgcgcgtc gctgatggcg ctgccggacc gcgggatcga tcgaccgttc atcgaccggg
      241 ggaatcctga tgcccatcct cgagcctcct tcccgctccc gggagccacc gcccccagcc
      301 gggatcctcg tgcacccgcc cgtcgcgcgc gacgaggcgg ccccggcaaa acgtcatcac
      361 cgggcctccc acgaccgcca tgccggcgta gggggtccac ccggctttgc tgaccacctg
      421 gtcgtcctgg atcacccggg agcgcccagg atcgaccagc gtgacatcgg cgtccgcgcc
      481 gggccagagg ctgcccttgc gcggatagaa gccgtacagc cgggccggac gctcgcacag
      541 ggcgtccacc acccgctcga gcgtcaccca tccttgatgg accgcgtgga gcatcagcgt
      601 gagggtggtc tcgacccccg gcagcccgaa gggcgcctgc cagatgttgc ctcgctcctt
      661 ctgctgccgg gtggcgggcg cgtgatcggt cgccaggtgc gtgatcgcgc cgtccgccag
      721 gaggccccac atccgcgcgg catcctccgc gctcctgagg cgtgccggcg gggtgaactt
      781 cctgaacggc ccatgccgca ccacttcatc ctcgcggagg aagaagtact gggggcagct
      841 ctccacccag aggcggcccc cgcgggcgcg ctcacgggcg atgagctcga ccgcctccgg
      901 gtggctcgtg tgggcgatca cgacgcgcgg accggccagg cgcgcgagca gcgcggtccc
      961 cgccgccgcc acctcctcgg cctcccgcga ccgccactcg ggaacgaccc cgaaatcggt
     1021 ccggccggcc tcccggagcg tgcgctctcg ctcgcgggtg atggcgtcgt cctcgcagtg
     1081 gaccaccagc agcccgtcgc acgccgccgc cgctcggaag cactgcagga gcgatccggc
     1141 cagcattgcc gggacgccgt gggtctcgca ggtgaacacc ttgaggtacg ccgcgccggc
     1201 cccccacagc tcggggatct gggacagacg atccggccag acgtgcgccg ccaggccgaa
     1261 gtcgacgacc gatctccggc ccaggtgcgc gaccttgcgg cgcaggccct ccgcatcgag
     1321 cacgggggcg gcgtgcgtgt gctcgatcac cgtggtggtg ccccccaccg ccgccgcggc
     1381 gctccccgtg ataaaatcct cgcgctcggt ctcgccggga tccatgaagt ggacgtgggt
     1441 gtccaccacc cccggaatcg cgcaaaggcc gcccgcgtcc acgacctgcg cggaggccgc
     1501 atcgagcgcg cccagcgcca cgatccgccc gtcgtggacg tgaatgtccg cggagaatcg
     1561 cccccgggag gtcaccaccg tcccgccgcg gatcgcgaga tcgatcacgg ccctcacccg
     1621 gccacccgca cgacgacccg gccggtggcc cggtcctcgc ggaccgcggc gagcgcctcg
     1681 ttcgcctgct cgagcggaaa cgtccccgag atcaccggcc ggacgaggcc gcgggcgacc
     1741 aggtccaagg cccgttgcag cccgaggcgc gtggtgtacc gcgtgcccaa gaccgagagt
     1801 tgccccagga cgacccggtc ggtcgggagc accgggtact cctcggggct ggtggcatac
     1861 cccacgacca cgatccgccc gccggtccgc acgagcccgc cggcggtcgc cagcgagcca
     1921 tggtgcccca ccgtatcgat caccacgtct gcgccgcggc ccgcggtggc agcccggacc
     1981 cgctcgcccg cgcccgcgcc ggcggggaac gcgtcgtgcg cgccggccgc ccgcgccgcc
     2041 tccaggcggt gggcgtggag atcgacggcc agcacgaccg ccccggccgc gcgtccgatc
     2101 tgcaccgcct gcaggccgac gccgccggcg ccgagcacca ccatcgcttc ccccgcgcgg
     2161 acgcctccgc gcgacacgac cgcctggtag gcggtcccga gggcgcaggt cagcgggccg
     2221 ccgtgttccg gcgggatgtt cgccgggagc ggcaggacgc agtcgtcggg cacggcgagg
     2281 tactcctgga atccgccggg cgtgccaaac ccggcccatc cgcggaggtt ggcgcagagg
     2341 ttctcctcgc cggccaggca gtgggcgcag gcccggcacg cccagtagtg gtagacgacg
     2401 acccggtccc cgatgcggcc gctggcgtcg ggcccgagcg cggcgatttc tcccgagatc
     2461 tcgtgccccg gcacgtgggg gagcggcagg gtcgaggaaa acggcatcgc ccccgccacc
     2521 accttcaggt cggaccggca gaccccacac ccgatcaccc ggacgagcac ctgacggggt
     2581 cccggcgccg gcatggggag gtccaccatc ttgagcgggg ccccgtaacg ctcgaccacc
     2641 atcgccttca tcgcccgacc tccgagattg ccggaccctt cgctactctt cgaacacgac
     2701 cgctccccgc ccgaacagcc gaccctcccg cagcatcgcg tggagggact ccacctccga
     2761 gagcgcgacc gtctgggaca cgaccggccg gatccggccg tcccgcacca ggcgggcggc
     2821 ggccaggacc tcggcgcggc tgcagtagcg ggagccgagg atcttgagct cgtccaccac
     2881 cagacgcctg gggagcaccc ggaaccccac gtcggcgaac gtcgtcagcg tcaccatccg
     2941 gcccccgcgc ccgaggagcc cgaacgccca tgccagcgtg tgttcgttgc ccacgaggtc
     3001 cacggccacg gtggcgccgc cgtcgagggc cgcccgagcc tgtgcctccg catccgaagc
     3061 ccggaagttg acggcgtgtg tagccccgag ttcgcggcac cgggcgagct tggcgtcgtt
     3121 cacgtccacg gcgaccacgt gcgcgccgaa gagccgggcc atctggagca tgtggatccc
     3181 gacgccgcct ccggcgccga tgaccagggc ccgatccccg acccgaacgc gcgccctggt
     3241 cgcgcacacg tggtacggcg tggcgatcgc atcggggatc gccgtcccct cgacaaacgg
     3301 cacaccctcc ggcagcggga gcacgttcgc ctcgggcagc gcgacgaacg ccgcatagcc
     3361 accgtcgcgg tggaccccca cgtatccgcc gtggtcccgg cagagggaat cccgtcccgc
     3421 ccggcagaac tcgcaccgcc cgcacgtcag gtagaagaac accagcaccc gatcccccgg
     3481 ccggaccgtc gccacccccg gcccgatctc ggcgacgacg ccggtgatct cgtgccccgg
     3541 gacccgcggc aggttgtcgg gacgcgccat ctggccgttc atgtaattca gcaccgtcag
     3601 tcccacgccg catgcccgga ccgccaccag gacctcgccc gcctgcggcg ttgggcgggg
     3661 gatctcgacg agcgtgagcg gacctcccca ggagtggatg acctgagcct tcaccagcgc
     3721 tcgcccccgg tcgcggtgga tcccatcatg cgggcccgac gcaagatggc aaggtcatcg
     3781 tgatgcactc atttcgggag ggcgaaatgc cggtcctcgt tggcccggac gtgcagcacc
     3841 cctaggacgc cccgcggatg acccgggcag ccttgaggag cagcgaccgg ttggccccat
     3901 ccgagtggag gaacgccgcg gcgtcgcgta ccagcttctc gatcccagca tcgcgcatgt
     3961 agccatgccc cccgtgcagc tccagggtgc gggtcaccac ccaccaggcc acgcgcgacg
     4021 cgaagacctt gggcagggcg cccagcgtcg ggtccatcgg ctcgggccgg tcggcgctct
     4081 cggccgcggc gtgggtgtac gcgcgcgcgg cgtcgatgag catgcgcatc tcggcgaggt
     4141 cctgcgcggt cgtgtcgtgg tcgatgagcc gccgcccgcc ctggatgcgg tcccgggtcc
     4201 acaggagcgc acgctcgtag gccgcatcgg cgacccccag gacgctcgcg gcggcgtagg
     4261 cgttgctggc ggggaagaac tgccgcagga tcgcgaaccc ctggtgcacc cggccaagga
     4321 tctgatcgtc ggcgaggcgg cagtcccgga aaatcagctc ggcgttgttg acgaggcgct
     4381 cgcccatctt atcgtgcacg cgcccgatcg aaaacccggg cgtcgcccgg ggaacgagga
     4441 agcacgtgct cccctccacc aggctcgccg aggggtcggt ctgggcgaac acgagataga
     4501 gcatcgcccg gttcccgttg ctgatgaagt gcttcattcc gttcagcacc cacgcgccgt
     4561 cccgctgcac ggccgtggtg tggaattgga cgtccggggg gtcaaagggg atgatgtagt
     4621 cggacgaggt cgtgggctcg gtgatgccga tggccagcag tccccgtgga tccttcgcaa
     4681 acgatgggag gaagcggcct gcctgcgcgg acgtgcacag ccgctgaagc gtctgggcga
     4741 tcttgagggt ctgcgcaaag atcaccgaga cccccagatc cccccgggcc agctcctcga
     4801 tcaccatcgc ggtggtcagg gcgtccgccg cggctccccc aaactcggtc ccgagggtca
     4861 gcgtgcggat gcctgcgcgc gaggcctgct cgacgatctc ccaggagaag cagtcctcgg
     4921 gatgggggcg gcggtcgagc tccgcggcca ccggcaccac ggcctgggcg acgaactcgc
     4981 gcacgccctc gagcaggcgg cgcacggtct caggatgcat cgggtggccg accctctccc
     5041 gcgcgcggcg gacaggcggt cagcacgccc gcttgaacca gtcgaggatc tccgccccgg
     5101 tccaaaacag cacgcccgac ttcgctcgga gataggcgta gatcatgtcg aagtacttga
     5161 tgcggtgggg cacgccggtg atatacgggt ggaccgcgat cggcagaacc cgcgcgttcg
     5221 tcttgccttc ttcgaagagc gtgtcgaact gatcccgggt ccggcggaac agctcctccg
     5281 cagcatgatg ctgggccaga aacatcggaa tgtcgttgat ctcaagcgag tacggcaccg
     5341 agacaaggcg cccccgcttc acgcgcatct cgtacggctg gtcgtcgttc gcccagtcgg
     5401 cgacgtactc gatcccctcc tcggccagca ggtcgagggt gtcccacgtt tcggcgagcc
     5461 ccgggctgag ccatccccgc ggcgccgccc cggtcgcgcg tcggatcgac gccacggtcc
     5521 gccggatcgc cgcgcgctcg tccggctcga gggggagcgg gcgctggatg aacccgtggc
     5581 ccacgatctc ccacttgctc tcgagggcgg cctcaacgag ccgcgggtag gacgtgcaga
     5641 tcgatccatt gaccgccatc gccgcccgca cccggtggcg ggccaggacg tccctgaggc
     5701 gccagaaccc cacccgcaat ccatagtcgt accagccata gttcgtgata tcgggaacgc
     5761 gcgaaccccc gccgggaagc ggcaacacgg tccgcggcag agtccgcgag atgtcccact
     5821 cctccacgtt gacgatcgtc cagatggcga cccgggcgcc gccgggcagc accatcggtc
     5881 ttcgatccac gatcggtgag tacgggtacc tcggggccgg ggccgccgct cggcgcctcg
     5941 gggtccggcg tggcatctcc gctccaccgt caccgcgcgg tgtgcgcggg gatggtgccg
     6001 agatggacga ggatctcgtc tgtggtcagg acccacccca ggcagcgctt gaggttctcg
     6061 aggccgaaga cgtggaggtc cgctccgtac atgctggcga cgccgtcgct ggcgacgatg
     6121 acccgcaggt cccggttgaa cgcatcgaag gcggtgcaga gcacgcacgt gttcgtgttg
     6181 atcccacaaa tcaccacggt ctcggtcccg agcgaccgga gcaggacgtc caggtcggtc
     6241 ccgtagaagg cgctcagccg cttcttcgaa cggatcacga agtcgcccgg ctggggcgcc
     6301 agctcggggg ggacctccgt ctgcggggcg ccttcctggt tgtggtggag gatcgtgctc
     6361 cgccgccccg gggtgaggga ctgcttgacc gcttccacgg cgcgccagaa cgggttcccc
     6421 atgctctcca atccgggcgg cgaggtgcgc cggtacgtca ggagcacgta gatcaccggg
     6481 acgcccgcgc cgcgcgcccc gccgaggagg cgggcgaccg cggcgcgcac gcgggcggcg
     6541 tcgtccggcg acacgggcat cgtgccgatc tcggggtcca ggtggccgcg gtgcacgtcg
     6601 acggccagga ccgcggtgcg ccgtggatcg acggtcagct ggctgcgcag cgcctggacg
     6661 aactcctgcc gatcgaggat ctcagccatc ggtctcgcct cacgctccgg catctcggcc
     6721 ccgggccccc acgcgcgcct acggatgggc cggcggcagg tcccgggtgt acaccggttt
     6781 cttgaggtac tcgtccgggt tcgcggtcca gaactgcgag acgttcgggt aggtgaagat
     6841 caccgtattt tggggctggc cgttcacgat gtcgacccgg cggatgtact cgttctggat
     6901 cgggttgccc cattggtcca ggcgcaccgg cccgcgcggc gcgtcgggga gcacgacgcc
     6961 ccggaccgcg gcaacaaacg cttcgcggtc ggcgatgcgg ccgtgcaccg cctcgagccc
     7021 cttgatgaag aagagcgcgc ccgtgtaggc cccctccgaa tagtaggacg gcacgcgact
     7081 gtaggcccgc acgtacgcca ggacgaagtc gcggttcgcc tgggtggcga gcgctgcgct
     7141 gtagtgcagc gccgtgagga cgcctttcgg caggtcgcgc tcctggaaca ggatgtgctc
     7201 gtccgtgaac gtgccgttgc cgatcagcgg aatccggcct tcgagtccga agtcccgata
     7261 ctgctggagg aagcgcaggg cgtcgccccc gctgaaggcc gcgtacacgg cgtccacgtc
     7321 cctggggatg cggccgagat aggggctgta gtccggcgcc ccgatcggcg gccagaggtg
     7381 gagcacgacc cgcccgccgt tttgctcgaa ggtgtcttgg aacccctcga cgttctccca
     7441 gccaaacgcg aaatcgtagg cgatcgtggc gatccgtttg tacttcagca ccttgaccgc
     7501 gtactcgccg aacggatggt tgggctggct gctcgtccag ccggtccgga tgatccaggg
     7561 cgtgcggaga cgctgggaga ggtcgtcggc cgagacgatc ggatagatcg cggggatctt
     7621 ctgctcgatg atgtagtcgc gcaacgcgag cccggagtcg gcacgcaatg ggcccatcaa
     7681 aatgtccacc cggtcaaact ccacgagctt cctggctttg gtcagcgcct ggccgggcgc
     7741 cccctcgtct tcggtgagga cctggatctc ccgcccggcg accttgttgc cgacctggtt
     7801 gagggccatg gcgaggccgt tcgccatgtc ccggccgttt ggcgcgaaca ctcctgtgaa
     7861 cgggacgatc agcccgatct tgatcagccc ctgctgggcg atcaccgggc cgccgggcac
     7921 caggagcgcc agcaccaccg cgcatgcgac gaacacgtgc aggtactgcc tcatcatctg
     7981 cgtctccccc ctgcatgcct gcttgccggt ctacaccccc aagtaccggt gcttcacggt
     8041 cgcatccgcc gacatctcgg ccggcgtccc atggtacacc acgcgcccct tgctcagcac
     8101 gtagatctcg tcggcaatcg ccagcgccat cggaaggttc tgctccacca gcaggatgct
     8161 gagccccgcc cctttgagcc ggccgatcga ggccccgagg tcccggacga gcagcggcgc
     8221 gaggccctct gccggctcgt ccatcaacag gaggcggggg ttcgtcatga gcgcccgcgc
     8281 gatcgccagc atctgctgct ccccgcccga cagcatgctg ccgcggtgac cggtgcgctc
     8341 cccgagccgc gggaacagcc ccagcacgcg ctccatcgtc cacggccctc ccgtcggacc
     8401 gtcgtggact ggtccgccgt tgcgcgcggc gacggtgaga ttctcgatga ccgtgagcga
     8461 agggaagatc cgccggccct gcggcacgag gccgatgccg agccgggcga tctcgtggct
     8521 cggcaggcgg gtgatctccc ggccttcgaa cagcacctgc ccgcggcggg gcctggcaaa
     8581 gccgatgatc gatcggatga gggtggtctt gcccatgccg ttgcgcccga gcagcgccgc
     8641 caccgtcccg ggacgcacct cgaggctgac gccctgcagg acgtaggaat cgccgtagta
     8701 cgtatggatg tcgtcgaccc gcagcatggc catctaccgg ccaccccccg cgccgcgtcc
     8761 ggcgggcccc tgcggcgcgc cgagatagat ctcctgcacc gccgggttcg cccgcacctc
     8821 gtccttggag ccctgggcca ccacgcaccc gaagtgcagc acggtgatcg tctcg
//
LOCUS       DASQGH010000603         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_14156, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000603 DASQGH010000000
VERSION     DASQGH010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14156"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /gene="gcvPA"
                     /locus_tag="VEW91_12035"
     CDS             <1..1070
                     /gene="gcvPA"
                     /locus_tag="VEW91_12035"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HYM92350.1"
                     /translation="EFLTAYTPYQAEIMQGELQAIFEYQSLMCELTAMDVANASMYDG
                     ASATGEAASMAADLTKRRRILVSPAVHPEYRQVLRTFTSHLRIAVEDLPATGGITDPA
                     AAARAMTDDTAALLIQSPNFFGCLEDGEALAKAAHDRGALLIAAIAEPISLGLVRAPG
                     EYGADIVTGEGQALGNALNFGGPYLGMIATREAFVRRLPGRLVGRTVDTEGRPGYVLT
                     LQTREQHIRRARATSNICTNEALNALVAAVYLSTLGRAGIQHVAELNARKAHYARERI
                     TALRGYATPFAAPTFNEFVIRCPLPPEEINRRLLEHRILGGLPLGRFYPELGDCWLLC
                     VTEARTREEIDRLVGYLETLQ"
     gene            1067..2566
                     /gene="gcvPB"
                     /locus_tag="VEW91_12040"
     CDS             1067..2566
                     /gene="gcvPB"
                     /locus_tag="VEW91_12040"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYM92351.1"
                     /translation="MSAPVRPPGPTAPPLSRRPVPVIFERGAPGRVGYSLPPSDVPEM
                     PLDAIIPAGRRRIRPAALPEVSELDVVRHYTALSHRNYSIDEGFYPLGSCTMKYNPKI
                     NEDIARQPGLARLHPYQPEDLVQGALQLLWELEHDLAEISGMDRVTFQPAAGAHGEMA
                     ALLMIGRFFEERGERRTTVVVPDSAHGTNPASAATAGFQVVEVRSDRRGNIDLEALRA
                     VLTPQVAALMLTNPNTLGLFEEQIREVARAVHNVGAQLYLDGANFNAILGITRPGDQG
                     FDVMHFNLHKTFTTPHGGGGPGAGAVGVRAHLVPYLPVPTIERDGQRFALDYDRPKTI
                     GKIRAFYGNFGNLVRAYTYIRSMGAPGLREAAEVAVLNANYMQRRLRDHYDLPYERIC
                     KHEFVLSGRRQRDRHHVTTKDMAKRLLDYGFHSPTIYFPLIVEEAIMIEPTETESIQT
                     LDAFVDAMIAIAHEAETNPEIVRSAPHETPVTRLDDVTAARHPILRWRP"
     gene            2717..3295
                     /locus_tag="VEW91_12045"
     CDS             2717..3295
                     /locus_tag="VEW91_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92352.1"
                     /translation="MIRPLRTRPPHTHFVSPLWYGLPRGNSAIEYAVALAIIGIVVTL
                     AWGWQGSITRRRVQNAAYLIEGDLRFAQQTAVATAGQGPQAELCLRSTGYDIYTVVYQ
                     DPVGRSMPAPGSIVKRVNAGQEYPGGIQITPDAFATYACTADGARQAIVFLASGAPEF
                     PDTNTHAIAVALGNQTAHITIQPTTGLATVGP"
     gene            3324..3869
                     /locus_tag="VEW91_12050"
     CDS             3324..3869
                     /locus_tag="VEW91_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92353.1"
                     /translation="MMRSRPRGRADGGFSMMEVQVAIVLLTVISLASWSTINSTLALM
                     GSTPSWSSQKTARVWTVASGWEQAELEYAKQLGYGGGCASPPCTIWVQMSGGNIQVST
                     DGCGTWATGAVPFSEGPPLPAAEFSQGRIVITTDPNTPTDPYSGATYLQDIEVDIFNK
                     VQANCASPSPYMSAYTSAGIR"
     gene            3886..>4124
                     /locus_tag="VEW91_12055"
     CDS             3886..>4124
                     /locus_tag="VEW91_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92354.1"
                     /translation="MSMRRLGRPQGYALYEVLVALSLLGLVSLAVFSAFKAGTTIWTT
                     SQQFVAEQQNARTLINTIARSVRMIGFQYGGASSAV"
BASE COUNT          678 a         1480 c         1382 g          584 t
ORIGIN      
        1 ccgagttcct gacggcctac acgccgtacc aggcggagat catgcagggg gagctgcagg
       61 cgatcttcga gtaccagagc ctgatgtgcg agctgaccgc gatggacgtc gccaacgcgt
      121 ccatgtacga tggcgcgagc gcgaccggcg aagcggccag catggcggcg gatctcacga
      181 agcgccggcg gatcctcgtc agccccgcgg tccaccccga gtaccggcag gtgctgcgca
      241 cgttcacgag ccacctgcgc atcgcggtgg aggacctgcc ggcgacgggg gggatcacgg
      301 acccggcggc cgccgcgcgc gccatgacgg acgacaccgc cgcgctcctc atccaatccc
      361 ccaacttctt cggctgcctt gaagacggcg aggcgctggc gaaggccgcg cacgaccgcg
      421 gggcgctcct gatcgccgcg atcgccgaac cgatcagcct gggcctggtc cgcgcccccg
      481 gggagtacgg cgccgacatc gtcaccgggg agggccaggc cctcgggaac gcgctcaact
      541 tcggcggccc gtacctcggc atgatcgcca cgcgcgaggc attcgtccgc cgcctgccgg
      601 gccggttggt gggccggacg gtggacaccg agggccggcc ggggtacgtg ctgaccctcc
      661 agacccggga gcagcacatc cggcgcgcgc gggcgacgag caacatctgc accaacgagg
      721 cgctcaacgc gctcgtcgcg gcggtgtacc tgtccaccct ggggcgggcg gggatccagc
      781 atgtcgcgga gctcaacgcg aggaaggcgc actacgcccg ggagcggatt accgccctcc
      841 gcggctacgc cacccccttc gccgcgccaa cgttcaacga gttcgtgatc cgctgcccgc
      901 tgccccccga ggaaatcaac cggcggctcc tcgagcaccg gatcctcggc gggcttcccc
      961 tcggccggtt ctatccggag ctcggcgact gctggctgct gtgcgtgacc gaagcgcgca
     1021 cgcgcgagga gatcgaccgg ctggtcggat atctggagac cctccagtga gcgcgcccgt
     1081 ccgtcccccc gggcccactg cgccgccgct gtcccggcgc ccggttccgg tcatcttcga
     1141 gcggggggcg ccgggccgcg tggggtacag cctgcccccg agcgacgttc cggagatgcc
     1201 gctcgacgcg atcattccgg cgggccgccg ccgcatccgg cccgcggccc tcccggaggt
     1261 cagcgagctg gacgtcgtgc ggcactacac cgccctcagc caccggaact actcgatcga
     1321 cgagggcttc tatccgctcg ggtcgtgcac gatgaagtac aatcccaaga tcaacgagga
     1381 catcgcccgt cagcccgggc tcgcgcgcct ccatccgtat caacccgagg acctggtgca
     1441 gggcgcgctc cagctgctct gggaactgga gcacgacctc gcggagatca gcggcatgga
     1501 ccgcgtgacc tttcaaccgg ccgcgggcgc gcacggcgag atggccgccc tcctgatgat
     1561 cggccggttc ttcgaggaac ggggggagcg ccggacgacc gtcgtggtgc ccgactcggc
     1621 gcacggcacc aaccccgcgt cggccgcgac cgcaggattc caggtcgtcg aggtcaggag
     1681 cgaccgccgg ggcaacatcg acctcgaggc gctgcgggcc gtcctgaccc cccaggtcgc
     1741 ggcgctgatg ctcacgaacc cgaacaccct ggggttgttc gaggagcaga tccgcgaggt
     1801 cgcccgcgcg gtccacaacg tcggcgcgca gctctacctc gacggcgcca acttcaacgc
     1861 gattctggga atcacgcggc cgggcgacca ggggttcgac gtcatgcact tcaacctgca
     1921 caagacgttc accacgccac atgggggcgg cggccccggc gcaggggcgg tgggcgtccg
     1981 ggcgcacctg gtcccctacc tgccggtgcc cacgatcgaa cgggacgggc agcggttcgc
     2041 gctggactac gatcggccga agaccatcgg caagatccgc gccttctatg gcaacttcgg
     2101 caacctggtg cgcgcttaca cgtacatccg gtcgatgggc gcaccgggac tccgcgaggc
     2161 cgccgaagtg gcggtgctga acgccaacta catgcagagg cgcctccggg accactacga
     2221 cctcccgtac gagcgcatct gcaagcacga gttcgtgctg tccggccggc ggcagcggga
     2281 ccgccaccac gtcaccacca aggacatggc gaagcgcctg ctcgactacg gcttccactc
     2341 gccgacgatc tacttccccc tgatcgtcga ggaagcgatc atgatcgagc cgacggagac
     2401 cgagagcatc cagacgctcg atgcgttcgt cgacgcgatg atcgccatcg cccacgaggc
     2461 cgaaacgaat ccggagatcg tccgcagcgc cccccatgag acgcccgtca cccggctgga
     2521 cgacgtgacg gcggcgcgcc atccgatcct gaggtggcgg ccctaggcgc tcgctgcggg
     2581 agacccccag aggttgaccc gcgactcccg agagattctc gggaggtttt cccccgcaaa
     2641 cccccgcccc ggcctttctg catgtccgcg ttcgcgcgcc ccggggtata caagtctctg
     2701 gatggggggg gtctccatga tccggccctt acggacaaga ccgccacaca cccattttgt
     2761 gtccccgctt tggtatgggc tcccgcgggg aaactccgcg atcgagtacg ccgtcgcgct
     2821 ggccatcatt ggcatcgtgg tcaccctggc gtggggatgg cagggctcga tcaccaggcg
     2881 gcgggtccag aacgccgcct acctcatcga gggagacctc cggttcgccc agcagacggc
     2941 cgtggccacg gccgggcagg ggccgcaggc ggaactgtgc ctgcgttcaa ccggctacga
     3001 tatttacacg gtggtctacc aggaccccgt cggccggtcg atgccggcgc cgggatcgat
     3061 cgtcaagcgg gtgaacgcgg ggcaggagta cccgggggga atccagatca cacccgacgc
     3121 ctttgccacc tacgcgtgca ccgccgacgg cgcgcgccag gcgatcgtgt tcctggcctc
     3181 gggcgcgccg gagtttcccg acacgaacac ccacgccatc gcggtcgcgc tcggaaacca
     3241 gaccgcccac atcaccatcc aacccacgac gggcctggca acggtcggcc cgtgaggcct
     3301 caacgcgaaa ggggtaccgc ccgatgatgc gatccaggcc tcgggggcgg gccgacggcg
     3361 ggttcagcat gatggaggtg caggtcgcga tcgtcctcct gaccgtgatc tcgctggcgt
     3421 cctggagcac catcaacagc acgctcgccc tgatggggag cacgccgtcg tggagcagtc
     3481 agaagaccgc ccgcgtgtgg acggtggcga gcggctggga gcaggccgaa ctggagtacg
     3541 cgaagcagct cgggtacggc gggggctgcg cgtcgccgcc gtgcacgatc tgggtccaga
     3601 tgtccggcgg gaacatccag gtctccaccg atggctgcgg gacctgggcg accggcgcgg
     3661 tgccgttctc cgaaggcccc ccgctgcccg cagccgagtt ctcgcaggga cggatcgtga
     3721 tcacgacgga ccccaacacg ccgaccgatc cgtattccgg ggccacctac ctccaggata
     3781 tcgaggtgga catcttcaac aaggtgcagg ccaactgcgc cagcccctca ccgtacatga
     3841 gcgcctacac ctcggccggg atccggtgac ggccgaaggc cgcgcgtgag catgcgacgc
     3901 ttgggccgcc cccagggata cgcgctgtac gaggtgctgg tcgccctgtc gctactcggg
     3961 ctggtctcgc tcgcggtatt ttccgcgttc aaggccggca ccacgatctg gaccaccagc
     4021 cagcagtttg tggccgagca gcaaaacgcc cgcaccctga tcaacaccat cgcccggagc
     4081 gtccggatga tcgggttcca gtatggcggc gcgagctccg cggt
//
LOCUS       DASQGH010000604         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53456, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000604 DASQGH010000000
VERSION     DASQGH010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53456"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1739)
                     /locus_tag="VEW91_12060"
     CDS             complement(6..1739)
                     /locus_tag="VEW91_12060"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYM92355.1"
                     /translation="MPGAGPVDPVASPGPATFASLAATLEDVGTASGTRAKVDRLAHY
                     LATLEEADLRLACTYLVGAALPPGDPRRLQVGGAAIARVLEEITGATAEELHRVYLAH
                     GDLGETAAALLHRRRPVPPLFPRPLTLRAIADAFGAIAAAQGPASRRTKQAALRALLL
                     DGTPLEAKFLVKILTSDLRVGLKEGLLLPAIARAFDRSVEAVRQAALLVADTGEVAVR
                     AKAGTLDRATLVVGSPFRFMLAGAIATLDEAFEGGGPPLLVEDKYDGIRVQVHRTGDR
                     LVLYSRTLEDVTGAYPELHAPLRRLGRAYILDGELVAWRGDRPLPFSALQKRLGLKAP
                     GAVAREIPVVLQAFDILHLDGSDLLQAPLETRRATLERLPWGGAVRLSDGVIAQGPAE
                     AEVRYRQARAAGHEGIILKRRDAPYQPGRRGRLWVKWKPGLASLDVVVVAAEYGHGKR
                     AGVLSDYTFAVRAGDDLVTIGKAYSGLTDAEIARLTQWFLEHTVQDLTGMRLVEPRIV
                     LEVAFDRIMQSDRHTSGFALRFPRILRVREDKPPEEINTLDDVRTLYLHTAHPGGPSG
                     PPGGKEQGSRA"
     gene            complement(1709..2530)
                     /locus_tag="VEW91_12065"
     CDS             complement(1709..2530)
                     /locus_tag="VEW91_12065"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HYM92356.1"
                     /translation="MKVFISADMEGTAGVTDWEQVLPEHLDYARFRRLMTEEVNAAIL
                     GALEGGAKEIVVNDSHNTMRNLLIEELHPQTQLISGSPKPYSMMQGIDNSFDAVFFTG
                     YHAAAGTQNGILDHTYSSATVRQLKLGNLVVGEAGLNAALAGHFKVPVALVTGDATAV
                     AQAKKLLGGAEAVAVKEPIGRLAARSYQPVEARRRIKEGATRALKRAKDLRPLILPRP
                     VMMEMEWMVTAMADRCLLIPGMTRVGPRTVAYKARDAEQGFSLFMACLVLARSIQ"
     gene            complement(2668..3417)
                     /locus_tag="VEW91_12070"
     CDS             complement(2668..3417)
                     /locus_tag="VEW91_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYM92357.1"
                     /translation="MPRDRPRIGVAGGDPAQLQNYLEAVEAAGGTAVPLLPGVARSPE
                     VLLDDLDGLVLTGGRDVDPALYGQRLQEGCGVELDAPRDALEIPLARRALERDLPVLG
                     ICRGVQVLNVAAGGTLHQDVTLVGLAQGSHNQRAAAPPPPEDAAVHEVTITPGSRLAQ
                     IFGDGRLGVNTFHHQVIDRPAAEFAVTARSVEPQGNGVIEAVEIPGRSFAVGVQWHPE
                     RMWRRVPACARLFSALVSAASRKRVARARPG"
BASE COUNT          557 a         1319 c         1287 g          566 t
ORIGIN      
        1 ctgttctacg cccttgaacc ctgctccttt ccccccggtg gcccgctggg gccgccgggg
       61 tgcgcggtgt ggaggtacag cgtccgcacg tcgtcgagcg tgttgatctc ctcgggcggc
      121 ttgtcctccc gcacccggag gatccgggga aagcgcagcg cgaaccccga ggtgtggcgg
      181 tcgctctgca tgatccggtc gaacgccact tccagaacga tccgcggttc gaccagccgc
      241 atgccggtga gatcctggac ggtgtgctcg agaaaccact gggtgaggcg ggcgatctcg
      301 gcgtcggtca accccgagta ggccttgccg atggtgacga gatcgtcccc cgcgcggacg
      361 gcgaaggtgt aatccgacag caccccggcc cgcttcccgt ggccgtattc ggcggccacg
      421 acgacgacgt ccagcgacgc cagcccgggc ttccacttca cccacaaccg cccgcgccgg
      481 cccggctggt acggggcgtc gcggcgcttc aggatgatgc cctcgtgccc cgccgcccgc
      541 gcctgccggt agcggacctc ggcctcggcg gggccctggg cgatcacccc gtcgctcagt
      601 cggacggctc caccccaggg gaggcgctcc agggttgcgc gtcgcgtctc gagcggcgcc
      661 tggagcaggt cgctgccgtc gaggtgcagg atgtcgaacg cctggagcac gaccgggatc
      721 tcccgggcca cagcccccgg ggccttgagc cccagccgct tctggagcgc ggagaacggc
      781 agcgggcggt ccccgcgcca ggccacgagt tcgccgtcca ggatataggc ccttcccagc
      841 ctccgcagcg gcgcgtgaag ctcggggtac gccccggtca catcctccag cgtcctcgag
      901 tagaggacga gccggtcgcc ggtccggtgc acctggacgc ggatgccgtc gtacttgtcc
      961 tccaccaaga gcggaggccc acctccctcg aaggcctcgt cgagcgtggc gatcgctccg
     1021 gcgagcatga agcggaaggg ggatcccacg acgagcgtgg cgcggtcgag cgtccccgcc
     1081 ttggcccgga ccgccacctc gcccgtatcc gccaccagca gcgccgcctg ccggactgcc
     1141 tcgacggacc ggtcgaaggc cctggcgatc gccggcagga ggagcccttc cttgagaccg
     1201 acccgcaggt cggaggtgag gatcttcacg aggaacttcg cttcgagggg agtgccgtcg
     1261 agcagcagcg cccgcagcgc cgcctgtttg gtgcggcggg acgccggtcc ctgggcggcg
     1321 gcgatggcgc cgaacgcgtc ggcgatcgcc cggagcgtca gcgggcgggg gaagagcggg
     1381 gggaccggcc ggcggcggtg gagcagcgct gccgcggtct cgccgaggtc tccgtgggcc
     1441 aggtagacgc ggtgcaattc ctcggcggtg gccccggtga tctcctcgag gacgcgggca
     1501 atcgccgccc cgccgacctg gagacggcgc gggtcgcctg gaggaagcgc ggcgcccaca
     1561 agatacgtgc aggcgaggcg gagatccgcc tcctccaggg tcgcgaggta gtgcgcgagc
     1621 cggtcgactt tcgcgcgcgt gccggaggca gtgccgacgt cttccagggt agcggccagg
     1681 ctcgcaaagg tcgcgggacc gggggacgct actggatcga ccgggccagc accaggcacg
     1741 ccatgaagag gctgaagccc tgctccgcgt cgcgggcttt gtaggcgacg gtgcgcggcc
     1801 cgacccgggt catgccgggg atcagcagac accggtccgc catcgccgtg accatccatt
     1861 ccatctccat catcaccggc cgggggagga tgagcggccg gaggtccttg gcgcgcttga
     1921 gggcccgggt cgccccctcc ttgatcctcc ggcgcgcctc gacgggctgg tacgatcggg
     1981 cggccaggcg cccgatgggc tccttgaccg cgaccgcttc ggcccccccg aggagcttct
     2041 tcgcctgcgc cacggcggtg gcgtccccgg tcaccagggc cacgggcacc ttgaagtgcc
     2101 cggccagcgc ggcgttgagc ccggcttcgc ccaccaccag gttccccagc ttgagctgcc
     2161 gcaccgtcgc gctgctgtag gtgtggtcga ggatcccgtt ctgggttccc gccgctgcgt
     2221 ggtacccggt gaagaacacg gcatcaaacg agttgtcgat cccctgcatc atgctgtagg
     2281 gcttggggct cccgctgatc agctgcgtct gcgggtgcag ctcctcgatg aggaggttcc
     2341 gcatcgtgtt gtgggaatcg ttgacgacga tctccttcgc gcccccctcg agcgcgccga
     2401 ggatcgcggc gttcacctcc tcggtcatga gccgccggaa ccgggcatag tcgagatgct
     2461 cgggcagcac ctgctcccag tcggtcaccc ccgccgtgcc ttccatgtcc gctgagatga
     2521 agaccttcat gaatcccccc tgtgggatcg cgctgagaga cccgggacga atacgtgcct
     2581 gggccctagg ataccttctc cagccggaag atggggtgca tgggagccgt cgatcctggg
     2641 accgcgccgg acctcaacga gcgggggcta tccaggccgc gcccgggcaa cccgctttcg
     2701 ggacgccgcg ctcacgagcg cggaaaacag ccgggcgcac gccgggacgc ggcgccacat
     2761 ccgctccgga tgccactgga cgccgacggc aaaggaccgc cccgggatct ccaccgcctc
     2821 gatcacccca ttgccctgag gttccaccga cctggccgtg accgcgaact ccgcggcggg
     2881 acggtcgatg acctgatggt gaaacgtgtt caccccaagc cgcccgtcgc caaagatctg
     2941 cgcgagccgg cttccgggcg tgatcgtcac ctcgtgcacc gccgcgtcct cggggggggg
     3001 aggcgcggcc gctcgttgat tgtggctgcc ctgcgcgagg ccgacgaggg tgacatcctg
     3061 gtggagcgtg ccgcccgcgg ccacattcag cacctggacg ccgcggcaga tgccgagcac
     3121 cggaaggtcg cgctcgagcg cccggcgggc cagcgggatc tcgagcgcgt cccgcggggc
     3181 atcgagttcg actccgcacc cttcctggag ccgctggccg tagagcgcgg gatcgacatc
     3241 cctgccgccg gtcagcacga gcccatcgag atcgtcgagc aggacctccg gggatctcgc
     3301 tacgccgggg aggagcggca ccgctgtgcc cccggccgcc tcgaccgcct caaggtagtt
     3361 ctgcagttgg gcggggtcgc ccccggccac gccgatccgc gggcggtctc gcggcacagg
     3421 ctcccggtct agacgctgcg acacggtacc actctactcg acttcccgaa ccaagtcaaa
     3481 acgtcgtctc gcgtcatcta atatcttacc tttcacgggc atttcgctat ggggaccgcc
     3541 ctcaaggaag ttagtcgcca agcgtgcctc gccttgcgtt tggccgacat agccatcaaa
     3601 atagccttat cagaaacacc gggggatgcg caggcgtgaa ggtggccaac accgttgaac
     3661 tcaagaacag gaccaatgca ctacttcgac aggtgatgcg gggcgagccg gtcatcgtga
     3721 cctatcgtg
//
LOCUS       DASQGH010000605         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_48078, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000605 DASQGH010000000
VERSION     DASQGH010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48078"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1030
                     /gene="rpsB"
                     /locus_tag="VEW91_12075"
     CDS             209..1030
                     /gene="rpsB"
                     /locus_tag="VEW91_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HYM92358.1"
                     /translation="MPDVTMKQLLEAGVHFGHQTRRWNPKMAPFIFTQRNGIHIIDLQ
                     KSVPLIEGAYKFVRETAAAGGSLLFVGTKKQAQDAIREEAIRAHQFYVNQRWLGGMLT
                     SFNTIKSRIDRLKELAEIRDNGTLEILPKREQAHLMDELARLEKYLGGIATMRTLPAA
                     MYIVDTRKEHIAIAEARKLRIPIVAIIDTNCDPDEADYPIPGNDDAIRAVRLITNRVA
                     NAAQEGYEEWRKAQIVEEEEVLAAAEEGVEPAPMAPLPGDGEVVEEAVEEEEVQA"
     gene            1048..1644
                     /gene="tsf"
                     /locus_tag="VEW91_12080"
     CDS             1048..1644
                     /gene="tsf"
                     /locus_tag="VEW91_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HYM92359.1"
                     /translation="MQISASLVKELRARTGAGVIDCRNALAESGGDLEKAQQVLRARG
                     LAAAARKGGRVAKEGLVTSYIHGEGRLGVLVEINCETDFVARTQDFKTLAREIALQVA
                     ASDPRYVVREDVPPDVAAAERAAYLTKARAEGKPEAQAQAEAEKQMEAFFQATCLLDQ
                     PSIRDAGRSVGVLIKEVIAKTGENIQVRRFARFRVGEE"
     gene            1641..>2323
                     /gene="pyrH"
                     /locus_tag="VEW91_12085"
     CDS             1641..>2323
                     /gene="pyrH"
                     /locus_tag="VEW91_12085"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018660093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYM92360.1"
                     /translation="MTGIARAAYRRVLLKLSGEALAGSEGTGLDPKVLHLVAREVKSV
                     RDDGVDVAIVVGGGNIVRGVQIAEHMGLNRATADYMGLLATVINALALQDAMEREGLE
                     TRVQTAVEMRQVAEPYIRRRAIRHLEKGRVVVFAGGTGSPYFTTDTAAALRALEIEAE
                     AVLLAKSGVDGIYDKDPRGNSDAVRFDTLEYMEVLNRGLKVMDATATSLCMDNDLPIV
                     VFDIIHAGNL"
BASE COUNT          432 a          721 c          846 g          324 t
ORIGIN      
        1 cggccgggcg gctcccccgg ccgggcgcgc gcgttccccc cgggggggtg cggtgtcgcc
       61 cagagatttg tcccgcctgg agagcctgtg ctatactacg tcaggacttt cagcacgctc
      121 tcggactccg ccggtggtgc ccgacgataa cgggttccgg tgggggatga agggagcgga
      181 ggcggaaccg gaaaggggat ggaacgcagt gccggatgtg accatgaagc aactcctcga
      241 ggcgggcgtc cacttcgggc accagacccg ccgatggaac cccaagatgg ccccctttat
      301 tttcacgcag cgcaacggca tccatatcat cgacctccag aaatcggtcc ccctgatcga
      361 gggggcctat aagtttgtgc gggagaccgc cgcggcgggg ggctcgctcc tgttcgtggg
      421 caccaagaag caggcgcagg acgcgatccg cgaggaagcg atccgcgccc accagttcta
      481 cgtcaaccag cgatggctcg gaggcatgct caccagcttc aacacgatca agagccggat
      541 cgaccggctg aaggaactgg cagagatccg cgacaacggc acgctcgaga tccttcccaa
      601 gcgcgagcag gcccacctga tggacgagct ggcgcgtctg gagaagtacc tcggcgggat
      661 cgcgacgatg cgaacccttc ctgccgcgat gtatatcgtc gacacgcgca aggagcacat
      721 cgcgatcgcc gaggcgcgca agctgcgcat cccgatcgtc gcgatcatcg acacgaactg
      781 tgaccccgat gaggccgact acccgatccc ggggaacgac gatgccattc gcgcggtgcg
      841 gctgatcacc aaccgggtcg cgaacgccgc ccaggagggc tacgaggagt ggcggaaggc
      901 ccagatcgtc gaggaggagg aggtgctcgc cgcggccgag gaaggcgtgg agccggcacc
      961 gatggctccg ctcccgggtg atggcgaagt ggtggaagag gccgtcgagg aggaagaggt
     1021 tcaagcataa cgcggaggat cgcaccgttg cagatcagcg caagtctggt gaaggagctc
     1081 cgcgcccgga cgggcgcagg ggtgatcgac tgccggaacg ccctcgcgga gtcggggggg
     1141 gatctggaga aggcccagca ggtgctgcgc gccagagggt tggcggccgc ggcccgaaag
     1201 ggcgggcggg tcgccaaaga gggcctggtg acctcgtaca tccacggcga gggtcggctg
     1261 ggcgtgctcg tcgagatcaa ctgcgagacc gatttcgtgg ctcggacgca ggacttcaag
     1321 accctggccc gagagatcgc gctccaggtc gctgcgagcg acccccggta tgtcgtccgg
     1381 gaggacgtcc ccccggacgt ggccgcggcg gaacgggcgg cctacctgac gaaagcccgc
     1441 gccgagggga agcccgaggc ccaggcccaa gcggaggcgg agaagcagat ggaagcgttt
     1501 ttccaggcga cctgcctgct ggaccagccg tccatccgcg acgcaggccg gtcggtgggc
     1561 gtgctcatca aggaagtcat cgcgaagacc ggcgagaaca tccaggtccg gcgcttcgcg
     1621 aggtttcggg tgggtgagga atgaccggga ttgcgcgcgc cgcctaccgg cgcgtgctcc
     1681 tcaagctgag cggcgaggcg ctggccggct ccgaaggcac cgggctcgac cccaaggtcc
     1741 tgcacctggt ggcccgcgag gtcaaatcgg tccgggacga cggggtggat gtggcgatcg
     1801 tggtcggcgg ggggaatatc gtgcgcggcg tccagatcgc cgagcacatg gggctcaacc
     1861 gcgccaccgc cgactacatg ggcctcctcg ccaccgtgat caatgcgctg gcgctgcaag
     1921 acgcgatgga acgcgagggc ctcgagaccc gggtccagac cgcggtggag atgcgccagg
     1981 tcgcggagcc ctacatccgg cggcgggcga tccggcatct ggaaaagggc cgggtggtgg
     2041 tcttcgcggg gggcaccggg agcccgtact tcaccaccga caccgcggca gcgctccggg
     2101 cgctggagat cgaggccgaa gcggtcctgc tggccaagag cggggtcgac gggatctacg
     2161 acaaggatcc ccggggcaac tcggatgcgg tgcggttcga cacgctggag tacatggaag
     2221 tgctcaaccg gggcctcaag gtgatggacg ccaccgccac atcgctgtgc atggataacg
     2281 atctgccgat cgtggtgttc gacatcatcc atgccggcaa tct
//
LOCUS       DASQGH010000606         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50822, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000606 DASQGH010000000
VERSION     DASQGH010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50822"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VEW91_12090"
     CDS             <1..257
                     /locus_tag="VEW91_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HYM92361.1"
                     /translation="STVAAMQSGILFGFAGQVDGVVERMQGELGGRAAVVATGGFAEL
                     VAPEARSIAHVDPLLGLEGLRILYERNTAPEAAGEAPRRA"
     gene            267..1187
                     /locus_tag="VEW91_12095"
     CDS             267..1187
                     /locus_tag="VEW91_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HYM92362.1"
                     /translation="MKRVVSVSLGSSTRDKRVETDILGERFVIERIGTDGNTARYRAL
                     VEELDGRVDAIGMGGIDLYLRAGSARYRVRDAARLIRGVRRTPVVDGGGIKNSWEKYL
                     ILEYLPKEAGISFRDRRVLLVSSVDRYGMAEAFTQAGAKTLFGDFYFALGVPIPMRSL
                     TTVRLLAACLLPVFTKLPFKLLYPTGAKQDQNTPRYAHLFRWAEVVAGDFHFIRRYMP
                     PDLTGKTVLTQTVTRQDVEELRRRKTQTLITVSPEMDGRSFATNVLEGIVLALSGRRS
                     EDLRPEEYVAWLLRAGFRPRIETLTPAGAR"
     gene            1184..2299
                     /locus_tag="VEW91_12100"
     CDS             1184..2299
                     /locus_tag="VEW91_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYM92363.1"
                     /translation="MSAPSTGRFVFVLHPLYVSQFGNKFPIARYLPGRLVEAAFRAVP
                     PFEASHITGIVSATGARAEGWFIALPWTPRILLAAPPDLVYRRLIQAGRIAERLGAGI
                     LGLGAFTKVVGDRGVTVAAALGIGVTTGNSLTVATAIEGALLGATRMGIEVPKAQVAV
                     VGATGAIGAACSKILARQVSGLILVARTRSRLEALAEQLRSVGSARIEVTSDVRRAVG
                     AADIVITVSTATDVLIEPEDLRPGAVVCDVARPRNVSRLVYDRRGDVLVIDGGVIEVP
                     GPVDFGLDFGFPPRSCEACMAETIILALEGRYEDYTLGGDVHLDRVDEIHALMRKHGF
                     HLSGFRRFERRISDEEVEAIRQAARPPGAPRPVSLGL"
BASE COUNT          331 a          758 c          861 g          369 t
ORIGIN      
        1 gcagcacggt ggcggccatg cagtcgggga tcctgtttgg cttcgccggc caagtggacg
       61 gtgtcgtgga gcggatgcag ggggaactgg gcgggcgggc ggcggtggtg gcgaccggag
      121 ggtttgccga gctcgtcgcg cccgaagccc gatcgatcgc gcacgtggat ccgctgctcg
      181 ggctcgaggg cctgcgcatc ctgtacgagc gcaacaccgc acccgaggcg gcaggagagg
      241 ccccccggcg ggcctaacgg ggtgcgatga agcgcgtggt cagcgtgagc ctggggagca
      301 gcactcgcga caagcgcgtc gaaacggaca tcctgggcga gcggttcgtc atcgagcgga
      361 tcggcaccga cggcaacacg gcccggtacc gggccctggt ggaggaactc gacggccgcg
      421 tggacgcgat cgggatgggc ggcatcgacc tgtacctccg cgccgggagc gcacgctatc
      481 gggtgcgcga cgccgcgcgg ctgatccggg gcgtccgccg cacaccggtg gtcgatgggg
      541 ggggcatcaa gaactcatgg gagaagtacc tgatcctcga gtacctgccc aaagaggccg
      601 gcatctcgtt cagggaccgc cgggtgctgc tcgtgagcag cgtcgaccgg tacggcatgg
      661 ctgaggcctt cacgcaggcc ggcgccaaga cgctgttcgg agatttctac ttcgccttgg
      721 gggtgcccat cccgatgcgg tcgctcacga ccgtccggct gctggccgcg tgcctgctgc
      781 cggtgttcac gaaactcccg ttcaagctcc tctacccgac cggggcgaag caggaccaga
      841 acaccccgcg ctatgcgcac ctgtttcggt gggccgaggt cgtggcggga gattttcatt
      901 tcatccgccg gtacatgccg ccggacttga ccgggaagac ggtgctgacc cagacggtga
      961 cgcgtcagga tgtggaggaa ctccggcgcc gcaagacaca gaccctcatc accgtgtcgc
     1021 cggagatgga cggccgctcc tttgccacga acgtcctgga ggggatcgtc ctggcgctct
     1081 cggggcggcg gagcgaggac cttcgcccgg aggagtacgt ggcgtggctg ttgcgggccg
     1141 ggttccgtcc gcggatcgag acgctcacgc cggccggagc tcgatgagcg ccccctctac
     1201 gggacggttc gtgtttgtcc tgcatccgct gtacgtgtcg cagttcggga acaagttccc
     1261 gatcgcccga tacctgcccg ggcggctcgt ggaggccgcg ttccgcgcgg tgccgccgtt
     1321 tgaggcctcc cacatcaccg ggatcgtgtc tgcgaccggc gcccgggccg agggctggtt
     1381 tatcgcgctg ccctggacgc cgcggatcct gttggcggct ccacccgacc tcgtctaccg
     1441 gcgcttgatc caggccggcc ggatcgccga gcgcctgggg gcggggattc tgggcctggg
     1501 ggcgttcacc aaggtggtcg gcgaccgggg ggtgaccgtt gcggctgcac tcggcatcgg
     1561 ggtcactacg gggaacagcc tcaccgtcgc caccgcgatc gaaggcgcgc tattgggcgc
     1621 gacgcggatg ggcatcgaag tccccaaggc ccaggtcgcc gtcgtgggag cgaccggcgc
     1681 catcggcgcg gcgtgctcca aaatcctcgc gcgccaggtg agcggcctca tcctggtggc
     1741 ccggacccgg agccgcctgg aggcgctggc ggagcagctc cggtcggtgg gctcggcgcg
     1801 catcgaggtc acctcggacg tccgccgcgc tgtgggagcc gccgatatcg tcatcacggt
     1861 cagcaccgcg accgatgtgc tgatcgaacc ggaggacctg cgcccgggcg cggtggtgtg
     1921 tgacgtggcg cggccacgca acgtctcgcg gctggtctat gaccgccgcg gcgatgtcct
     1981 cgtgatcgac ggcggggtga tcgaggtccc ggggccggtc gactttggat tggactttgg
     2041 gttccccccg cggtcatgcg aggcgtgcat ggcggagacg atcatcctcg ctctggaggg
     2101 acgctacgaa gactacacgc tcggcgggga cgtgcacctc gaccgcgtgg atgagatcca
     2161 cgcgttgatg cgcaagcacg ggttccacct ctcggggttc aggcggttcg agcgccggat
     2221 ctccgacgag gaggtcgagg cgatccggca ggccgcacgg ccccccggcg ccccgcgccc
     2281 ggtctcgctc gggctgtagc ggggactacc ctcctagcc
//
LOCUS       DASQGH010000607         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_39636, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000607 DASQGH010000000
VERSION     DASQGH010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39636"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..837
                     /locus_tag="VEW91_12105"
     CDS             133..837
                     /locus_tag="VEW91_12105"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYM92364.1"
                     /translation="MAVALARTIRNGEVVAQGVNSVLASLAILLARATHAPALTWLTV
                     AGGIDPAISTLPDSSVSPAWLAGTRAILSNPGFYRLAMRGGIDRMFLGAAQIDRRARL
                     NLSVIGDPAHPRVRLPGGGGAAVLAQVVKRIVVWRTRHTPEVFVPEVDFVTAQGNVEA
                     VVTPLGVLGLQHGCLVLQARFPGVALEEVQARTGFPVLPQAPVPVLEPTEEELRIVRA
                     LDRRRQRDLEFPRDAG"
     gene            complement(868..>1924)
                     /locus_tag="VEW91_12110"
     CDS             complement(868..>1924)
                     /locus_tag="VEW91_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYM92365.1"
                     /translation="GKTNTPEFGCKVFTDNRLFGATANPWALDRSAGGSSGGAAAAVA
                     AGLGPLAEGSDLAGSIRHPASWCGVVGFKPSQGRIPRYPNLTAWNGMSSNGPITRTVA
                     DAALMFAVMAGPDPRDPLALPDTGEDWTRLPDHPDLRGLRVAWAPDLGGAAPVDPSVA
                     SICQAATRVFAGLGCRLEDASPEIGNIRDPFLALNAALRQATIGKYLDQWREQMDPIL
                     VRRLELGKALTAADVGRAEIERSAYHQRLRRFFERYDLLLLPTTAIPASPLDALLPKE
                     IAGRAIRDHLDMVVQTYAFNLSNHPAISVPCGWTSGGLPVGLQIVGGWRRDALVLKAA
                     ASFELAAPWTDRRPPLH"
BASE COUNT          276 a          665 c          705 g          278 t
ORIGIN      
        1 atgcccgact atgacgtgga tccgggaggg atcaacgcgt acctccaggc ggccgggtcc
       61 gacgaagccc tggagggata cctggaccgc gtgaacccgt aaccacggat gtcgcccgac
      121 gcctctgaat tgatggcggt ggccctggcc cggaccattc gcaatggcga ggtggtggcg
      181 caaggggtca acagcgtcct ggcctcgctg gcgatcctcc tggcccgggc cacgcacgcg
      241 cccgcactca cgtggctcac ggttgccggc ggcatcgatc cggccatttc gaccctgccg
      301 gactcctcgg tcagccccgc gtggctcgca ggcacccgcg ccattctcag caacccgggg
      361 ttctaccggc tggccatgcg ggggggtatc gaccggatgt tcctgggagc ggcgcaaatc
      421 gaccggcgcg ctcgattgaa cctcagtgtc atcggggacc ccgcgcatcc ccgcgttcgc
      481 ctgccgggag gcgggggcgc cgcggtgctc gctcaggtcg tgaagcgcat cgtcgtctgg
      541 agaacccggc ataccccgga ggtcttcgtc ccggaggtgg attttgtgac cgcgcagggg
      601 aacgtcgagg cggtcgtcac gcccctcggg gtgctgggcc tccagcacgg ttgcctcgtg
      661 ctgcaggcgc gctttcccgg ggtcgcgctc gaagaggtcc aggcccggac gggctttccc
      721 gtgctcccgc aggcgccggt gccggtcctc gagccgaccg aagaagagct gcgcatcgtg
      781 cgcgccctgg atcgaaggag gcagcgggac ctcgaatttc cccgcgacgc cggctgagcg
      841 ggtgcggtcc gccgcccccg cgggcgttca gtggagcggt ggcctgcggt cggtccacgg
      901 cgccgccagt tcaaaactgg ccgccgcctt gaggaccagc gcatcccgcc gccagccgcc
      961 gacgatctgc agccccaccg gcagcccccc cgacgtccac ccacacggga ccgaaatcgc
     1021 ggggtggttg ctcaggttga aggcgtaggt ctggaccacc atgtcgagat ggtcccggat
     1081 ggcgcggccg gcgatctcct tcgggaggag cgcgtccagc ggcgaggccg ggatcgcggt
     1141 ggtggggagc agaagcaggt cgtaccgttc gaagaagcgc cgcaggcgtt gatggtacgc
     1201 cgaccgctcg atctctgccc gaccgacatc ggcggcggtc agcgccttgc cgagctcgag
     1261 ccgccggaca aggatcgggt ccatctgttc gcgccactgg tcgaggtact tgccgatggt
     1321 ggcctgccgg agcgcggcgt tgagggcgag gaacggatcc cggatgttcc cgatctcggg
     1381 ggaagcgtcc tccaggcggc acccgagtcc ggcgaacacc cgggtggcgg cctggcagat
     1441 cgaggccacc gacgggtcga ccggggccgc cccgccgagg tccggcgccc aggccacccg
     1501 cagcccgcgg aggtcggggt ggtccggaag ccgggtccag tcctccccgg tgtcggggag
     1561 ggcgagcgga tcccgcggat cagggccggc catcaccgcg aacattaagg cggcgtcggc
     1621 aacggtgcgg gtgatcgggc cgttcgagga catgccgttc caggcggtca ggttcgggta
     1681 gcgcgggatc cggccctggg aaggcttgaa ccccacgacc ccgcaccagg aggcagggtg
     1741 gcggatcgag ccggcgaggt cgctgccctc cgccagggga cccagtccgg ctgcgaccgc
     1801 ggccgccgcc ccgccgctcg atccgccggc ggagcggtcc agcgcccacg ggttggccgt
     1861 cgcgccaaac aggcgattgt cggtgaacac cttgcagcca aactccgggg tgttcgtctt
     1921 gccg
//
LOCUS       DASQGH010000608         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5403, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000608 DASQGH010000000
VERSION     DASQGH010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5403"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /gene="rpsM"
                     /locus_tag="VEW91_12115"
     CDS             complement(<1..137)
                     /gene="rpsM"
                     /locus_tag="VEW91_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYM92366.1"
                     /translation="MARIAGVDLPREKRVQVALTYVYGIGTSRSNEILQITGVNPATR
                     VR"
     gene            complement(147..260)
                     /gene="rpmJ"
                     /locus_tag="VEW91_12120"
     CDS             complement(147..260)
                     /gene="rpmJ"
                     /locus_tag="VEW91_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYM92367.1"
                     /translation="MKVRASVKRMCEKCKIVKRGNRVMVICENPKHKQKQG"
     gene            complement(282..500)
                     /gene="infA"
                     /locus_tag="VEW91_12125"
     CDS             complement(282..500)
                     /gene="infA"
                     /locus_tag="VEW91_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HYM92368.1"
                     /translation="MAKKDVIEVEGTGVEELPNAMFRVELSSGHKVLAHISGKMRIHF
                     IRILPGDRVKVELSPYDPTRGRITYRFR"
     gene            complement(504..815)
                     /locus_tag="VEW91_12130"
     CDS             complement(504..815)
                     /locus_tag="VEW91_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HYM92369.1"
                     /translation="MARYNDDLRVLAMTSPAGLLGRLATSRSGRDAGAVYLVVGAESA
                     TVVLVADGRQRSVARPKRKNVKHLELGAPSPAIAERLAAGPPLTDVDIRAGIAAGMPP
                     G"
     gene            complement(802..1566)
                     /gene="map"
                     /locus_tag="VEW91_12135"
     CDS             complement(802..1566)
                     /gene="map"
                     /locus_tag="VEW91_12135"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYM92370.1"
                     /translation="MILLKSPTDLAVMRRAGEIAARALQAVVRAVRPGITTRELDRIA
                     EDRIRRLGAEPAFLGYRGFPASLCTSVNDEVVHGIPGPRRLHEGEIISLDLGAVLEGF
                     HGDLAVTVPVGKVSPAVARLLRVTAEALEVGIRAVRPEGHLGDVSAAIQRYVETHGFS
                     VVREFAGHGIGRHLHEEPQIPNFGRPGNGVALRPGMTLAIEPMVNMGTSEVVMDADGW
                     TVRTRDGKPSAHFEHTVAVGDDGPIVLTGLPPDGSV"
     gene            complement(1580..2242)
                     /locus_tag="VEW91_12140"
     CDS             complement(1580..2242)
                     /locus_tag="VEW91_12140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HYM92371.1"
                     /translation="MNLVFMGPPGAGKGTQAELFRKRHELPHISTGDMFRQAVRDQTD
                     VGKRAWAYMQRGDLVPDEIVDELVRLRLTNPDSARGFVLDGYPRTLRQSEALHALLAQ
                     RREDLDAVVLFEIRDAVLLRRLTGRRVCSVCGAIYHLDHQPPRLPGRCDRDGSELMLR
                     KDDAPDTVLHRLEVFRQWTAPLVDYYRKRGLFLAVDAERTMEEVHERILEFVRSRAGR
                     GV"
BASE COUNT          338 a          876 c          753 g          357 t
ORIGIN      
        1 cgcacccggg tggccgggtt gaccccggtg atctggagga tctcgttcga ccggctcgtg
       61 ccgatgccgt agacgtacgt cagcgccacc tgcacccgct tctcgcgcgg gaggtccacc
      121 cctgcaatcc gtgccatccg tccctcctac ccctgcttct gcttgtgctt ggggttctcg
      181 cagatcacca tcacgcggtt cccccgcttg acgatcttgc acttctcaca catccgtttg
      241 accgacgccc gcaccttcac ggtccacccc ctcgccgcgc gctaccggaa gcggtacgtg
      301 atccgccccc gcgtaggatc gtagggcgag agctccacct tcacccggtc gcccggcagg
      361 atccggatga agtggatgcg catcttgccc gagatatgcg ccagcacttt gtggccggac
      421 gagagctcca cacggaacat cgcgttcggc agctcctcga cgcccgtccc ctcaacctcg
      481 ataacgtcct ttttggccat cggctatccc gggggcatcc cggcggcgat ccccgcccgg
      541 atgtctacgt cggtgagcgg tgggccggcc gcgagccgct ccgcgatcgc cggcgacggc
      601 gcccccaact cgaggtgctt cacgttcttg cgtttgggcc gcgcgacgct gcgctggcgg
      661 ccgtccgcca cgagcaccac ggtcgcgctc tcggccccga ccacgaggta cacggcgccc
      721 gcgtcccgcc ccgaccgcga ggtggccagc cgccccagca gccccgccgg ggacgtcatc
      781 gcgaggacac gcaaatcatc attataccga gccatcgggg ggaagtccag tgaggacgat
      841 cgggccgtcg tccccgaccg ccaccgtatg ctcgaagtgc gcggacggct tcccgtcgcg
      901 cgtccgcacc gtccacccat cggcatccat cacgacctcg gaggtcccca tattgaccat
      961 cggctcgatc gccagggtca tcccggggcg cagggcgacg ccgttgccgg gccggccaaa
     1021 gttcgggatc tggggctcct catggaggtg gcggccgatc ccgtggcccg caaactcgcg
     1081 gacgaccgaa aacccgtgtg tttcgacgta ccgctggatc gccgcgctca catcgccgag
     1141 gtggccttcc ggccggacgg cgcggatccc gacctcgagg gcctcggccg tgacccgcag
     1201 caggcgcgcg acggccgggc tgaccttccc aacggggacc gtcacggcga gatcgccgtg
     1261 gaacccctcc aggaccgccc ccagatccag gctgatgatc tcgccctcgt ggagccggcg
     1321 ggggccgggg atcccgtgga cgacctcgtc gttgaccgac gtgcagaggc tcgcggggaa
     1381 cccccgatac cccagaaacg ccggctcggc gccgagccgg cggatccggt cttccgcgat
     1441 ccggtccagt tcgcgcgtcg tgatgcccgg ccggaccgcc cggaccaccg cctggagcgc
     1501 gcgcgcggcg atctccccgg cacggcgcat caccgcgagg tcggtcgggg atttgaggag
     1561 gatcacggcc ggcgccccac tagacccccc gcccggcccg ggaccgcacg aactccagga
     1621 tgcgttcgtg gacttcctcc atggtccgct cggcgtccac ggcgagaaag agcccgcgct
     1681 tccggtagta gtccacgaga ggcgccgtcc actggcggaa gacctccagg cggtggagga
     1741 ccgtgtccgg cgcatcgtcc ttccgcagca tcagctcgga cccatcccgg tcgcaccggc
     1801 ccgggaggcg cgggggctgg tggtccaggt ggtagatcgc cccgcagacg gagcacaccc
     1861 gccggccggt gaggcggcgg aggaggaccg cgtcccggat ctcgaacaac accacggcgt
     1921 cgaggtcctc gcggcgctgg gccagcagcg catgcagcgc ctcggactgg cgcagcgtcc
     1981 tgggatagcc gtccaggacg aatccccggg cgctgtccgg gttggtcagc cggagcctga
     2041 cgagttcgtc cacgatctcg tcgggcacca ggtccccgcg ctgcatgtag gcccacgcgc
     2101 gcttgcccac gtccgtctgg tcgcggaccg cctgccggaa catgtccccg gtcgagatgt
     2161 gcgggagttc gtggcgcttc cggaacagct cggcctgcgt gcccttgccc gccccgggcg
     2221 gccccatgaa aacgaggttc atgccgcgcc cgtcacttca tgaacccttc gtagtggcgc
     2281 ctcagcacgt aggcctcgat ctgcttcatc gtctccaacg ccac
//
LOCUS       DASQGH010000609         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45174, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000609 DASQGH010000000
VERSION     DASQGH010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45174"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VEW91_12145"
     CDS             complement(<1..419)
                     /locus_tag="VEW91_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92372.1"
                     /translation="MGAMDKVPHRALPPTAPPVPKHTEHPLSLQAELAGTILEAARVL
                     SSGLAEHTTWSEAWQTIRDLEHKGDAIARDLFEAQAAPGPLPVSAELVQELTGYLDDV
                     LDAIEAAAARLAIHRIKRPLPAAREMGEIVLESAQQL"
     gene            538..1413
                     /locus_tag="VEW91_12150"
     CDS             538..1413
                     /locus_tag="VEW91_12150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYM92373.1"
                     /translation="MSERIGFIGLGIMGRPMAGHLVDAGYAVTVWNRTPSKTAGLVER
                     GASAADSPKDVAARSDITIIMVADTPDVLDVVLGSTGVLPGARPGSVVVDMSTISPVA
                     TREIARKLAERGVEMLAAPVSGGEKGAIEATLSIMVGGKPEVFERVLPVFQKMGKQIV
                     HLGESGAGQVTKACNQLVLSLTLLGVAEALTMAHKAGVDPAKVRAALLGGFAQSRVLE
                     LHGQRMLDRNFEPGFRTRLYHKDMGIVMETGRSVAMPLLGGALAAQLYQIAMNRGFGE
                     KDYSVLARVIADLAG"
     gene            1424..2350
                     /locus_tag="VEW91_12155"
     CDS             1424..2350
                     /locus_tag="VEW91_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYM92374.1"
                     /translation="MVMHLDEEGVRAVLRWDPLIAEMESALTAFSSGRVIQPMRTMIT
                     IEEGKRYLGIMPAVAPDVMGLKAVSFYPGNAGTALPTHLAMILLFRPETGEPLAVMDG
                     RLITEMRTAAVSAAVTKRLASPQSRVLALLGSGVQAHAHLEALSHVRRFEDVRVWSRT
                     PDHAKRFAQEHDHVRAMDAEAAVRGADVIVTATNAREPILKGAWLKPGAHVNAVGAAR
                     PTWRELDDDAMANVVVVDSREAVLKESGDVILSKAQIYAEAGEIFGGTKPAPTGATTV
                     FKSVGLAIEDIATARLVYEATRARHRRDGRTP"
     gene            2431..4137
                     /locus_tag="VEW91_12160"
     CDS             2431..4137
                     /locus_tag="VEW91_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYM92375.1"
                     /translation="MAATERRSRPPDLATPAAAKAEWTARELAQSLAQLPERRERFET
                     LSGLPVERIYGPDDVAGLDYATDLGFPGRYPYTRGPYPTMYRSRPWTMRQIAGFGTAP
                     DTNARLKYLIAQGQTGISVDFDMPTLMGHDSDDPRARGEVGREGVAVDTVDDLDEILD
                     GIDIGTISVSMTINPTAWILFAMYLAIADRRGVSRDVLSGTVQADILKEYIAQKEWLY
                     PIPAGMRIVRDLIIYSVRHLPHYNPINISGYHIREAGANAVQEAAFTLANGIAYVEAV
                     CKKGLAVDDFAPRLAFYFIAERDFFEEVAKFRAARRVWARLMRERFGATKPESMRLRF
                     HCQTAGSSLTAKEPLNNIARTALQALSAVLGGTQSLHTNGMDEALAIPSELAMKVALR
                     TQQVILEESGAANTIDPLAGSYFVETLTNRMEDGIWAYIRRIDELGGAVEAARLNFFQ
                     TELADTAYRYQKSRERGELAVVGVNKYADAAGASGLPLALQEVDPGVEERQKQRLARV
                     KHERDNHAVERCLAGLADVARGDDNLIPPTIEAVKAHATAGEIVQALRAVFGTYLEVP
                     VF"
     gene            4149..4565
                     /locus_tag="VEW91_12165"
     CDS             4149..4565
                     /locus_tag="VEW91_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HYM92376.1"
                     /translation="MAGRRLRILMAKVGLVGHDRGVKVVARALRDAGFEVVYTGLHRL
                     PEEVAEAAVQEDVDVIGVSILSGAHMALVPPLLKELRTKEAADIKVLVGGVIPPSDRQ
                     KLRDLGAAAVFDQDATTDEIVEWIRRELGEAAGGSR"
     gene            4552..>4811
                     /locus_tag="VEW91_12170"
     CDS             4552..>4811
                     /locus_tag="VEW91_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HYM92377.1"
                     /translation="MDPAEPWPPAYDDAYLPAPQQRHWFPARETMAPAERETVIVRRL
                     QAVVRWAWERSRFYRDRWTAAGVHPDHLRTLEDLGRFPVVSKA"
BASE COUNT          768 a         1636 c         1716 g          691 t
ORIGIN      
        1 ttgagctgct gcgcggattc gagcacgatc tctcccatct cgcgcgcggc gggcaggggc
       61 cgcttgatgc ggtggatcgc cagacgcgcg gcggccgctt cgatggcgtc caggacgtcg
      121 tcgaggtagc cggtcagttc ctgcacgagc tccgcgctga ccggaagggg gcccggggcc
      181 gcctgggcct cgaagagatc tcgggcgatc gcatcccctt tgtgctccag gtctcggatg
      241 gtctgccagg cctccgacca cgtggtgtgt tcggcgagcc ccgaggacag caccctggcg
      301 gcttccagga tggtgccggc cagttcggcc tgcaggctga gcggatgctc ggtgtgcttg
      361 ggcaccggag gcgcggtggg tgggagcgcg cggtggggga ccttgtccat ggcacccatg
      421 agttggccgc ctcccgccgg gactcctctc gggcatgaca ggaaacgacg cgcaccgtcg
      481 cgcattcaac cgtgtccgcg gcaacgcggg acgcgcgcaa agcgaggtga gggtggcgtg
      541 agcgaacgca tcggcttcat cggcctgggg atcatggggc gccccatggc cggacacctg
      601 gtggacgccg ggtatgcggt gacggtctgg aaccgcaccc cgagcaagac ggccgggctc
      661 gtggagcgcg gcgcatccgc cgccgactcg cccaaggatg tggccgcgcg aagcgacatc
      721 acgatcatca tggtggccga cacccccgac gtcctcgacg tggtcctggg atcgaccggc
      781 gtgctccccg gcgcccggcc cgggagcgtc gtcgtggata tgagcacgat ctcaccggtg
      841 gcgacccgcg agatcgcgcg caagctcgcc gagcgcgggg tggagatgct cgccgccccc
      901 gtctcgggtg gggagaaggg cgcgatcgag gccaccctgt cgatcatggt gggcggcaaa
      961 cccgaggtgt tcgagcgggt gctgccggtg ttccagaaga tgggcaagca gatcgtccac
     1021 ctcggcgagt ccggcgccgg ccaggtcacg aaagcgtgca atcagctcgt gctgtccctc
     1081 accctcctcg gcgtggcgga agcgctgacg atggcccaca aggccggcgt cgatccggcc
     1141 aaggtccggg ccgcgctcct cggagggttc gcgcagagcc gggtgctcga gttgcacggg
     1201 cagcggatgc tcgatcgcaa cttcgagccg gggttccgga cccggttgta ccacaaggac
     1261 atggggatcg tcatggagac cggccgatcg gtcgcgatgc cgctgctcgg gggagccctc
     1321 gccgcccaac tgtaccagat cgcgatgaac cgggggttcg gggagaagga ctactcggtg
     1381 ctggcccgcg tgatcgccga tctcgcgggg taggaggacc cccatggtga tgcacctgga
     1441 cgaggagggc gtacgggcgg tgctgcgctg ggatccgctc atcgccgaga tggagtccgc
     1501 gctcacggcc ttctcgtcgg gacgggtcat tcagcccatg cggaccatga tcaccatcga
     1561 ggaggggaag cggtatctcg ggatcatgcc cgcggtggcc ccggacgtca tgggcctgaa
     1621 ggccgtaagt ttctacccgg gcaatgccgg cacggcgctc ccgacccacc tcgccatgat
     1681 cctcctgttc cggccggaga ccggcgagcc cctcgccgtc atggacggcc gcctgatcac
     1741 ggaaatgcgc acggcggcgg tctcggcggc ggtcaccaag cgtctggcct ccccccagag
     1801 ccgggtcctc gccctgcttg gcagcggggt gcaggcccac gcgcacctcg aggcgctctc
     1861 gcatgtgcgc cgcttcgagg acgtgcgggt ctggagcagg acgcccgacc atgccaaacg
     1921 cttcgcgcag gagcacgacc acgtgcgggc gatggacgcc gaggcggcgg tccggggggc
     1981 agacgtcatc gtgaccgcca cgaacgcgcg cgaaccgatt ctgaagggcg cgtggctgaa
     2041 gcccggggcc cacgtcaatg ccgtgggagc ggcccgcccg acctggcggg aactggacga
     2101 tgacgcgatg gccaacgtgg tggtggtgga ttcccgcgag gcggtcctga aggagtcagg
     2161 cgacgtgatc ctctccaagg cccagatcta cgccgaagcg ggcgagatct tcggggggac
     2221 gaaaccggcc ccgaccgggg ccacgacggt gttcaagtcg gtcggcctcg cgatcgagga
     2281 catcgccacc gcccgcctgg tctacgaggc gacccgggcg cgacatcggc gcgacgggag
     2341 gacgccgtga ggcctgcgtt gccttgacgt agcggcggcc atctccctat actcacaccg
     2401 acaacgccca ccaaaggggg aggtgcgcac atggcagcga ccgagcgccg gagccggcct
     2461 cccgacctcg ccaccccggc tgcggccaag gccgagtgga ccgcacggga actcgcccag
     2521 tccctcgcgc aactccccga gcgccgggaa cgcttcgaaa cgctgtccgg gctccccgtc
     2581 gagcgcatct acggtccgga cgacgtcgcc gggctcgact acgcaacgga tctcgggttt
     2641 ccgggccgct atccatacac ccggggcccc taccccacca tgtatcggtc ccggccctgg
     2701 acgatgcgcc agatcgccgg gttcggcacg gcccccgaca ccaacgcccg gctcaagtac
     2761 ctgatcgcgc agggccagac cggcatctcg gtcgacttcg acatgccgac cctcatgggt
     2821 cacgattccg acgacccgcg cgcgcgcggg gaggtcggcc gcgagggggt cgcggtggac
     2881 accgtcgacg acctcgacga gatcctcgac gggatcgaca tcggcacgat cagcgtctcg
     2941 atgacgatca accccacggc ctggatcctc tttgccatgt acctggcgat cgccgaccgg
     3001 cgcggcgtct cccgggacgt cctctcgggc acggtgcagg cggacatcct gaaggaatac
     3061 atcgcccaga aagagtggct gtaccccatt cccgccggca tgcgcatcgt gcgggacctg
     3121 atcatctaca gtgtgcggca cctcccccac tacaacccga tcaacatcag cgggtatcac
     3181 atccgcgagg ccggggcgaa tgccgtccag gaagccgcct ttacgctggc caacggcatc
     3241 gcctacgtgg aagcggtctg caagaagggc ctcgccgtgg atgactttgc gccccgcctc
     3301 gccttctact tcatcgccga gcgcgacttc ttcgaagagg tggcgaagtt ccgcgccgcg
     3361 cggcgggtct gggcccggct catgcgcgag cggttcggcg caacgaagcc ggagtcgatg
     3421 cgcctccgct tccactgtca gaccgccggc tcgagcctga cggccaaaga gccgctcaac
     3481 aacatcgccc gtaccgccct ccaggccctg tcggcggtgc tgggcgggac ccagagcctg
     3541 cacaccaacg gcatggacga ggcgctcgcc atcccgtcgg agctcgccat gaaagtggcg
     3601 ctgcgcacgc agcaggtcat tctggaggag tcgggcgcgg ccaataccat cgatccgctg
     3661 gccggcagct acttcgtgga gacccttacc aatcgcatgg aggacggcat ctgggcgtac
     3721 attcggcgga tcgatgagct cggcggggcg gtggaagcgg cccggctgaa tttcttccag
     3781 acggagctgg ccgacacggc ctaccggtac cagaagagca gggaacgcgg cgagctggcg
     3841 gtggtcggcg tcaacaagta cgcagacgcc gccggggcat ccggcctccc cctggccctg
     3901 caggaggtgg atccgggcgt ggaagagcgg cagaagcagc ggctcgcccg ggtcaagcac
     3961 gagcgggaca accacgccgt ggagcgctgt ctcgccgggc tggccgacgt ggcgcgcggg
     4021 gacgacaacc tcatcccccc cacgatcgag gccgtcaagg cgcacgcgac ggccggggag
     4081 atcgtccagg ccctccgggc cgtgttcggg acgtacctgg aggtgcccgt gttctaggcg
     4141 ggggcgccat ggccgggcgg agactccgga tcctgatggc caaagtgggg ctggtcggcc
     4201 acgaccgcgg cgtcaaagtg gtggcccggg cgctccgcga cgccgggttc gaggtcgtct
     4261 acacgggcct ccaccggctg cccgaggaag tggccgaggc ggcggtgcag gaagacgtgg
     4321 atgtgatcgg cgtgagcatc ctgtccgggg cccacatggc gctcgtgccg cccctgctca
     4381 aggagctccg caccaaagag gcggccgaca tcaaggtgct ggtggggggc gtcatcccgc
     4441 cctccgaccg gcagaagctg cgtgacctgg gagcagcggc cgtcttcgat caggacgcca
     4501 cgaccgacga gatcgtcgag tggatccggc gagagctggg ggaggcggcc ggtggatccc
     4561 gctgagccct ggcccccggc gtacgatgac gcctacctgc ccgcgcctca gcagcgccac
     4621 tggtttcccg cgcgcgagac gatggcgccc gcggagcgcg agaccgtgat cgtgcgccgg
     4681 ctacaggcgg tcgtccgctg ggcgtgggag cggtcacgat tctaccgcga ccggtggacc
     4741 gccgccggcg tgcaccccga ccacctgcgg acgctggagg acctggggcg cttcccggtg
     4801 gtgtccaagg c
//
LOCUS       DASQGH010000610         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53498, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000610 DASQGH010000000
VERSION     DASQGH010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53498"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VEW91_12175"
     CDS             complement(<1..1325)
                     /locus_tag="VEW91_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYM92378.1"
                     /translation="MQPPPAVFREYDIRGVVGRDLDPEAAEAIACAFGTYLAASGVRR
                     AVIGHDNRTSSPALYEAAVRGLASTGCDVIKIGLAVTPMLYFALHHFGVDGGVMVTAS
                     HNPPEFNGFKLAHGPGTLYGAQIQEVRRLAETRAFRVGAGTVETRAILPAYAGMLRDR
                     IRLGPRHLRVVVDCGNGTAGAVVPEILRGWEVEVIPLYCDPDPTFPHHHPDPVDPRNL
                     RDLIQVVTEGRADAGLGFDGDGDRLGAVDDTGEILWGDILMALFWREILAAHPGALAL
                     VEVKCSQALIDEIRRLGGRPQFTQTGHSLIKARMRALGAVFAGEMSGHMFFADEYFGY
                     DDAIYAAGRLLRILSRTERPLSSLAGALPRFHTTPEIRVACPDDRKQQVVASLTREFA
                     ARYEVIDIDGVRVVFPDGWGLVRASNTQPVLVVRAEGTTPQALDRIKTVL"
     gene            1369..>2260
                     /locus_tag="VEW91_12180"
     CDS             1369..>2260
                     /locus_tag="VEW91_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYM92379.1"
                     /translation="MAASNLDDRRTINPMTEIRRLTGYIRPYAAHLVGGLVAAIMVAV
                     AWLYVPKYLGDQIDAVIRTGSYAVLNRAALIVLGIYIFRSVCLYIQLSLLAFVGHRLV
                     ADLRFAAFRQVQRWSLDRFLRWHSGEVISRTIQDTQLVESRLLNGLVDLLTTGLLVIG
                     VVVALFLIQWRLALLTFGTIGALIVAARIFGREVQQGSTRAQSRVASLTGLIKESIAG
                     ARIIRAFVQETREEKRFSRENERTFEENYRIRQLIALEVSLVSVLNALGLVFVLWAGV
                     AYVAKGAMTPGSLIAFLAYLA"
BASE COUNT          347 a          793 c          751 g          369 t
ORIGIN      
        1 agaacggtct tgatccggtc gagtgcctgg ggggtcgtcc cttcggcgcg gacgaccagc
       61 accggctggg tgttgctggc ccgcaccagc ccccatccgt cgggaaagac gacccggacc
      121 ccgtcgatgt cgatcacctc gtagcgcgcc gcaaactcgc gggtgagcga ggccaccacc
      181 tgctgctttc gatcgtcggg gcaggcgacc cggatctcgg gcgtggtgtg gaaccgcggg
      241 agcgccccgg cgagagatga gagcgggcgc tcggtgcgcg aaaggatccg cagcagccgg
      301 cccgccgcgt agatcgcgtc gtcgtacccg aaatactcgt cggcgaagaa catgtgcccg
      361 gacatctctc cggcgaacac ggccccgagg gcgcgcattc tggctttgat cagcgagtgg
      421 ccggtctggg tgaactgcgg ccgcccgccc agccggcgga tctcgtcgat cagggcctgg
      481 gagcacttga cctcgacgag ggccagggcg ccggggtgag cggcgagaat ctcccgccag
      541 aacagggcca tcaagatgtc cccccagagg atctcccccg tgtcatccac cgccccgagc
      601 cggtcgccgt cgccgtcgaa cccgagcccc gcatcggcgc gtccctcggt gaccacctgg
      661 atcaggtcgc gcaggttgcg ggggtcgacg ggatccggat ggtggtgcgg gaacgtggga
      721 tccgggtcgc agtagagcgg gatcacctcg acctcccacc cccggaggat ctcgggcacc
      781 accgcgccgg cggtcccgtt gccgcaatcg acgacgaccc gcagatggcg cggcccgagg
      841 cgaatccggt cgcgcaacat cccggcgtac gcgggcagga tcgcgcgcgt ctcgaccgtc
      901 cccgcgccca cgcggaatgc gcgcgtctcc gcaagcctcc ggacctcctg gatctgggcc
      961 ccgtacaggg tgccggggcc gtgggcgagt ttgaagccgt tgaactccgg agggttgtgg
     1021 ctggccgtca ccatcacccc gccgtccacg ccgaagtgat gcagcgcaaa gtagagcatc
     1081 ggcgtgaccg cgagcccaat cttgatgacg tcgcacccgg tactggcgag tccgcggacc
     1141 gccgcctcgt agagcgcggg ggaactcgtc cggttgtcat ggccgatcac ggcacgccgc
     1201 acgccggatg cggcgagata ggtcccaaac gcgcacgcga tcgcctccgc cgcttcgggg
     1261 tcgaggtctc ggcccacgac gcctcggatg tcgtactctc gaaagaccgc cggcggcggc
     1321 tgcatgcgta cgccccctcg atcgacgtca cgatacgtag gatcttcgat ggccgcgtcg
     1381 aaccttgacg accgtcgcac catcaaccct atgacggaga ttcgacggct caccggatat
     1441 atccggcctt acgcggccca cctcgtcggc ggtcttgtgg cggcgatcat ggtcgcggtg
     1501 gcctggctgt acgtgccgaa atacctcggc gaccagatcg acgcggtcat caggaccggg
     1561 agttacgccg tcctcaatag ggcggcgctc atcgtcctgg gcatctacat cttccggagc
     1621 gtctgcctct acatccagct gtcgctgctg gcgttcgtcg gccaccgcct cgtcgccgac
     1681 ctccggttcg cggcgttccg ccaggtccag cgctggtccc tcgaccggtt cctccggtgg
     1741 cacagcgggg aggtcatctc ccgcacgatc caggataccc agctggtcga atcccgcctg
     1801 ctcaacgggc tggtcgacct gctcaccacg gggctgctcg tcatcggcgt cgttgtcgcg
     1861 ctcttcctca tccagtggcg cctggcgctc cttacctttg gcaccatcgg agccttgatc
     1921 gtggcggccc gcatctttgg ccgcgaggtc caacagggct cgacccgggc gcagtcgcgc
     1981 gtggcctcgc tcaccgggtt gatcaaggaa tccatcgccg gggcacggat catccgggcc
     2041 ttcgtgcagg agacgcggga ggagaagcgc ttcagccgcg aaaacgagcg caccttcgag
     2101 gaaaactacc gcatccggca gctgatcgcg ctcgaagtct ccctcgtgag cgtgctcaac
     2161 gcgctgggcc tggtcttcgt cctctgggcc ggcgtggcgt acgtcgcgaa gggcgcgatg
     2221 accccgggat cgctgatcgc cttcctcgcc tacctggcgc
//
LOCUS       DASQGH010000611         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22377, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000611 DASQGH010000000
VERSION     DASQGH010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22377"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VEW91_12185"
     CDS             <1..1191
                     /locus_tag="VEW91_12185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HYM92380.1"
                     /translation="NTLRRLLPKQHRVVLVEQERQHAFAPSFLWLMVGARRHDQVVRD
                     LRTLTAPGVELIQAEVRGIDVAGQRVETTHKPISYDYLILALGAEYAPESVPGLDPTA
                     HTFYTWSGAAALQQALQAFPDRGGTVAVAICGLPYKCPGAPHEGAMLIADYFRRRGLG
                     RRVEVHLFTPEPQPLPVAGPTLGAAVQEMLRARNVIFHPLHKVSAVDGAAQRLQFEGR
                     ESVAFDLLVTVPPHRGSRVAREAGLTNDAGWVPVDRSTLRTRYENVYAVGDMTAIPIP
                     GRWKPDVPLLLPKAGIFAHAQGEVVAHRIAAEVVGAPAPTEFDGYGYCMLEAGGGIAG
                     MAFGNFFAEPSPQVELRRPGQVWHWGKVLFEQWWLAPPGVRRNALKVALALGARSLGL
                     RAVA"
     gene            1198..1938
                     /gene="nth"
                     /locus_tag="VEW91_12190"
     CDS             1198..1938
                     /gene="nth"
                     /locus_tag="VEW91_12190"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYM92381.1"
                     /translation="MLLRPHPSAALRRKALRADRILEAHYGPPARRRRLDPLSTLIGT
                     ILSQNTSDVNSHRAFERLRARFPSWKAVLNAPAGAVIEAIRPGGLGALKAVRIQTILR
                     RIVAERGVLSLDFLRRWPAARAKVWLRNLDGVGPKTAAIVLAFGLGKPAFAVDTHVYR
                     VGRRMGLIPERLSVEEAHRWMEALVKPARCGSFHLLLVRHGREICKAARPRCPLCPVR
                     QLCDFYRSTKRAHARVHYISRRSSGGRT"
     gene            1935..2669
                     /locus_tag="VEW91_12195"
     CDS             1935..2669
                     /locus_tag="VEW91_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM92382.1"
                     /translation="MIVDCELYYHGANESLGGKVFGIEALDELCREAGVDKAVLMPAP
                     TFYPRNQLVAEVLRSPAGARGRFVGCALVNPHLGGDAVGELERAVKEWGFRAAKLMPT
                     LHAINLVGRIAHPIMRKAQELRIPVTIHSAGSPAHPLEIGVLAEAFPDVPVIMDHMGY
                     RNYVGAAIAAAKRTANLYLMTTAVMEPHCIRDAVREIGAHRVVFGSNGPAIRPSTQIL
                     VIKQAELAPEDERKVLGDNAARLLGL"
     gene            2724..>3236
                     /locus_tag="VEW91_12200"
     CDS             2724..>3236
                     /locus_tag="VEW91_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYM92383.1"
                     /translation="MNPQTLREGEGVIMVGTDGSARNARGSAREVRAVSRRGLFEQSA
                     GAVAAGGLARWAAAAGAGTVATAFLATNAAAQQAQGNSRLYQVINRKMLIVGTGNGNP
                     PWHFQDEKGEFAGFDISMGRLLAGALFNDPTKVQFQIQAADARIPSLLTDKVDIVFQF
                     MTISALRAQQV"
BASE COUNT          508 a         1058 c         1125 g          545 t
ORIGIN      
        1 aacaccctgc ggcggctcct cccgaaacag caccgcgttg tgctggtcga acaggagcgc
       61 cagcacgcgt tcgcgccgtc gtttctgtgg ctgatggttg gagcgcggcg ccacgatcag
      121 gtcgtccggg acctccgtac cctcacggct ccgggcgttg agctcatcca ggccgaggtc
      181 cgggggattg atgtggccgg tcagcgtgtc gagacgaccc ataaacccat cagctacgat
      241 tacctgatcc ttgcgctggg tgccgagtac gcgcccgaat ccgtcccggg gctggatcca
      301 acggcacaca ctttctacac ctggagcggc gcggcggcgt tgcagcaagc cctccaggcg
      361 ttcccagacc gaggcgggac ggtggcggtc gccatctgcg ggctgcccta caagtgcccg
      421 ggcgcaccgc acgaaggcgc catgttgatc gccgactatt tccgccgccg gggcctcggc
      481 cgcagggtcg aggtgcacct gtttactccc gagccgcagc ccttgccggt ggcgggcccg
      541 acgctgggcg cggcggtcca ggagatgttg cgggcacgga acgtcatctt tcatccgttg
      601 cataaggtct cggcggtgga cggtgccgcg cagcgactgc agttcgaagg gcgggagtcg
      661 gtggcgttcg atctcctggt gacggtcccg ccccatcggg ggtcccgggt cgcgcgcgag
      721 gcggggctga cgaatgacgc cggctgggtg ccggtcgatc ggtcgacgct cagaacacgg
      781 tacgagaacg tctacgcggt tggcgacatg acggccatcc ccatccccgg gcggtggaag
      841 cccgacgtcc cgctgctgct gccgaaggcc gggatcttcg cccacgccca gggcgaggtc
      901 gtggcccacc ggatcgcggc ggaggtcgtc ggcgccccgg ccccaacgga attcgacggc
      961 tatggctact gcatgctgga ggccggaggg gggatcgccg ggatggcctt tgggaacttc
     1021 ttcgccgagc ccagcccgca ggtcgagctc cgacggccgg ggcaggtctg gcactggggc
     1081 aaggtgctct tcgagcagtg gtggctcgcg cccccggggg tacggcgcaa cgccctcaag
     1141 gtagcccttg cgcttggcgc acggtccctc ggtctgcggg ctgtggcctg aggggtgatg
     1201 ctcctgcgac cgcacccttc tgcggcgttg cgccgcaaag ctttgcgagc ggatcggatc
     1261 ctagaggccc attatggccc gccggcgcgc aggcggcggc tagacccact ctccaccctc
     1321 atcggcacga tcctgtccca aaacacaagc gacgttaata gccaccgggc cttcgagcgc
     1381 ttgcgcgccc gatttccgtc ctggaaggcc gtgctcaacg cgccggccgg agcggtcatc
     1441 gaggccatcc gccccggagg cctgggcgcc ctaaaggcgg tgcggatcca gaccatcctc
     1501 cggcgcatcg tcgcggaacg cggtgtcctc tcgctggatt tcctccggcg gtggcccgcc
     1561 gcgcgcgcca aggtatggct gcgcaacctc gatggtgtgg ggcccaagac cgccgcgatc
     1621 gtcctggcct tcgggttggg caagccggcg ttcgcagtcg acactcatgt ataccgcgtt
     1681 ggccgacgca tggggctcat ccccgagcgg ctgtctgtgg aagaggcgca ccgctggatg
     1741 gaggccctgg tcaagcccgc gcgttgcggg tcgttccacc ttttgctcgt ccgccacgga
     1801 cgcgagatct gtaaggccgc ccggccgcgg tgcccgctct gtcctgtacg ccagctgtgc
     1861 gacttctaca ggagtaccaa gcgagctcat gctcgtgtcc actatatatc caggcggtcc
     1921 tccggggggc ggacatgatc gtcgactgtg agctctacta tcacggggcg aacgaatcgc
     1981 ttggcggcaa agtcttcggg atcgaggcgc tggatgagtt gtgccgcgag gcgggggtcg
     2041 acaaggcggt tctcatgcct gcgcccacat tctacccgag gaaccaactc gtcgccgaag
     2101 tgctccgctc acccgcgggg gctcgggggc ggttcgtggg gtgcgcgctc gtcaatccgc
     2161 acctcggtgg tgatgcggtc ggcgagttgg agcgcgcggt caaggagtgg gggttcaggg
     2221 ccgccaaact gatgcccacg cttcacgcca tcaacttggt cggccgcatc gcgcacccca
     2281 tcatgcgcaa agcgcaggaa ctccggattc ccgtgacgat ccattccgcc ggctcccccg
     2341 cccatccgct cgagatcggg gtgctggccg aggcgtttcc cgatgtgccg gtgatcatgg
     2401 atcatatggg ctacagaaac tacgtgggcg ccgccattgc tgcggcaaag cggacggcca
     2461 acctgtatct catgaccacg gccgtgatgg agccccactg cattcgagac gccgtgcgcg
     2521 aaataggcgc gcatcgggtc gtgttcggtt ccaacggtcc cgccatacgg ccgtccacgc
     2581 agatcctggt gatcaaacag gcagaattgg cgcctgaaga tgaacgcaag gtgctgggcg
     2641 acaacgcagc gcgcttgttg ggcctatagc cgtgcaggaa acctcctcac ggcgatgaag
     2701 agtgcgaccg cgccggagtg gtggtgaatc cgcagacgct ccgggagggc gagggggtga
     2761 tcatggtggg gaccgacggc agtgcgagga acgcgcgtgg tagcgcgcgt gaggtccgcg
     2821 cggtatccag gcgggggttg ttcgagcaat cggcgggcgc ggtggccgcg ggcgggctgg
     2881 cccggtgggc tgccgcggcc ggcgcgggga cggtcgcgac cgcgttcctg gccaccaacg
     2941 ccgctgcgca gcaggctcag ggcaacagca ggctctacca agtgatcaac cgcaaaatgc
     3001 tgatcgtggg gaccggcaac ggcaaccccc cgtggcactt ccaggatgag aagggcgagt
     3061 ttgccgggtt cgacatctcg atgggacggc tcctcgccgg cgccctgttc aacgacccca
     3121 cgaaggtcca gttccagatc caggcggccg atgcgcggat cccaagcctg ctcaccgaca
     3181 aggtggacat tgtcttccag ttcatgacga tctccgcgtt gcgcgctcag caggtc
//
LOCUS       DASQGH010000612         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22385, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000612 DASQGH010000000
VERSION     DASQGH010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22385"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VEW91_12205"
     CDS             <1..809
                     /locus_tag="VEW91_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYM92384.1"
                     /translation="KEQGAVSQQDLDAAETQAQVAQAQVTQAEAAYDSARQNWNLTEV
                     GARQEDIQQARAQLAQAQAGLAAIQVQLRDSTVYAPFAGTITQRSVEPGEIVSSFAGS
                     AQSPLFVLSQVDDVYVEFVVPAQHRAELQQGQAAQMAVDGLPGKTFDGRVEEIRPAAD
                     VASRSFGVKIRVPNPQGILRPGMFARGAIVVGVRRDVLQIPEEAVVAATSGPIAFVAR
                     DGRVTRQSVTLGDTHDGLVEVKSGLAEGDKVVVQGQEALTDNQPVTLRTP"
     gene            828..>3755
                     /locus_tag="VEW91_12210"
     CDS             828..>3755
                     /locus_tag="VEW91_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYM92385.1"
                     /translation="MQWLAGLSVRRPVLASVITLAMVFLGVFSYAHLNIERWPNVDIP
                     IIVISVTDPGASPEEIESDVTNKIEDAVNSISGIDHLSSTSAESVSIVVVQFVLEKNI
                     DVAAQEVQNKINAIPDLPSGIDPPTVSKIDPSAFPVMTLALSANRPVRDISEYADKVL
                     KHQLEGTPGVAQVTLIGDQPRQINVWVDPDRMASYGLAVTSVLQAVQNQNVQLPAGTV
                     DQGNTQLTLRALSRIASPQELANVGLATRNGQPVRIGDVARVEDGAAEPVTTANVNGT
                     PAVLLQIQKQSGTNALNVVRDLKARLRAIGPQLAAGYNLRIVRDQSVFVEASTHAVQE
                     HLIIGSILAALVVLVFLWNWRSTLIAALAIPTSIIATFTLIAALGLTLNTITLLALAL
                     VVGIVIDDAIVVLENIYRLLREKGMPPVQAAIEGTREIGPAVTATTVSLIAVFLPLAF
                     MSGIVGRFMKSFGWTMAFAIAVSLIVSFTLTPSLSARWLGRGRKAGVSAVDSLGQEEG
                     VPAAPAAVEHDRGRVYNALDHGYRWLLEHALRRRWVVVVLTLVTLFSIGPLGTAVNKN
                     FLPQDDESQLEVVVRAPEGWTLDATNRFATQMATEIRKLAGIDYTIVTTGDDPQHTPN
                     RFTIFVKMVDLDKRSISQQAMEGKIRDLILSRYAYLGLQAQVEQVSEFGGSGGFQPVD
                     YVVSGPDLSVLTRAGDQGIQILRSIPGVVDPRSSLVSGAPQLGMQVDRARAADLGVNA
                     VDAANALFMLVQGVEVKTAKYAEAGQQYKIVIRADKQHRSDQEVLAQLPVSSPSQGVV
                     ALEQVVTASRGTGPSEITHYNRRRQVELTANLLPGASQQTVLQALDRKVRALGLGPQY
                     DMTFTGQAAEQGKQAAAFMQSFMLSFLFMYLVLAAQFESWIHPVTILLSLPLTVPFAL
                     LSILIFGQSVNIFTSLGILVLFGVVKKNSILQIDHANQLKDTGLSTHDAIVRASRDRL
                     RPIL"
BASE COUNT          619 a         1269 c         1266 g          601 t
ORIGIN      
        1 tcaaggagca gggcgccgtt tcccagcagg atctcgacgc cgccgagacc caggcgcagg
       61 tggcccaggc ccaggtcacc caggcggagg ccgcgtacga ctcggcccgg cagaactgga
      121 acttgacgga ggtgggggcg aggcaggagg acatccagca ggcgcgcgcc cagctggccc
      181 aggcccaggc cgggctggcc gccatacagg tgcagctgcg cgactccacg gtgtacgcgc
      241 cgtttgccgg gacgatcacg cagcggagcg ttgagccggg cgagatcgtg tcgtcgtttg
      301 ccgggtccgc ccaaagcccg ctgtttgtgc tgagccaggt ggacgatgtc tatgtcgagt
      361 tcgtggtacc tgcgcagcac cgggccgagt tgcagcaagg gcaggcggcg cagatggcgg
      421 tggacggcct cccggggaag acgttcgatg gccgcgtgga agagatccgg ccagcagcgg
      481 atgtcgccag ccggtcgttt ggcgtcaaga ttcgggtccc caacccgcag gggatcctcc
      541 ggccggggat gttcgcccgg ggcgcgatcg tggtgggcgt gcgccgtgac gtcctgcaga
      601 tcccggagga ggcggtcgtg gcggcgacct cgggtccgat cgcctttgtc gcgcgggacg
      661 ggcgggtgac ccggcagagc gtgacgctcg gagacactca cgacgggttg gtcgaagtga
      721 agtccggcct cgccgagggc gacaaggtgg tcgtccaggg ccaggaagcc ctcaccgaca
      781 accagccggt cacgctccgc accccgtagc gtcaaggagg atcgctcatg cagtggcttg
      841 ctggtttgtc ggttcgtcgc cccgttctcg cctcggtgat caccctggcg atggtcttcc
      901 tgggcgtctt ttcttacgcg cacctgaaca tcgagcgctg gcccaacgtc gacatcccca
      961 tcatcgtcat ctcggtgacc gatcccggcg cctcgccgga agagatcgag agcgacgtca
     1021 ccaacaagat cgaggacgcg gtcaactcga tcagcgggat cgaccacctc tcctcgacct
     1081 cggccgagag cgtctcgatc gtggtcgtgc agttcgtcct cgagaagaac atcgacgtcg
     1141 cggcgcagga ggtccagaac aagatcaacg ccatccccga tctcccgtcg gggatcgatc
     1201 cgccgaccgt gtccaagatc gatccgagcg cgttcccggt gatgacgctc gccctgtcgg
     1261 caaaccggcc ggtcagggac atctcggagt acgcggacaa ggtgctgaag caccagctgg
     1321 aaggcacgcc cggcgtggcg caggtgaccc tgatcggcga ccagccgcgg cagatcaacg
     1381 tctgggtcga ccccgaccgg atggcgtcct acgggctggc ggtgacgtcg gtgttgcagg
     1441 ccgtgcagaa ccagaacgtc cagctgcccg ccggcaccgt cgaccagggc aacacgcagc
     1501 tgaccctgag ggcgctcagc cgcatcgcgt caccgcagga actggcgaac gtggggctgg
     1561 cgacgcgcaa cggccagccg gtcaggatcg gggacgtggc ccgcgtcgag gacggcgcgg
     1621 ccgaacccgt caccaccgcg aacgtgaacg ggactccggc ggtgttgctc cagatccaga
     1681 agcagtccgg caccaacgcc ctcaacgtcg tgcgggatct caaggcgcgc ctgcgggcga
     1741 tcgggcccca gctcgcggcc gggtacaacc tccggatcgt gcgcgaccag tccgtgtttg
     1801 tggaggcgtc cacccacgcg gtgcaagagc acctcatcat cggctccatc ctggcggcgc
     1861 tggtggtgct cgtgttcctg tggaactggc gctccaccct gatcgcggcc ctggcgatcc
     1921 ccacctcgat catcgccaca tttaccctga tcgcggccct cggcctcacg ctgaacacga
     1981 tcacattgct cgccctcgcc ctcgtggtcg ggatcgtgat cgacgacgcg atcgtggtgc
     2041 tcgagaacat ctaccgtctg ctccgggaga aggggatgcc gcccgtccag gcggcgatcg
     2101 agggcacgcg cgagatcggc cccgccgtga ccgccaccac ggtgagcctg atcgcggtgt
     2161 tcctgccgct ggcgttcatg agcgggatcg tcggccggtt catgaagagc ttcggctgga
     2221 cgatggcgtt tgcgatcgcc gtctcgctga tcgtgagctt cacgctcacc ccctcgctct
     2281 cggcccgctg gctgggccgg ggcaggaagg ccggggtgtc ggcggtggat tcactggggc
     2341 aggaggaagg cgtgccggct gcccccgcgg cggtggagca cgaccgaggg cgggtctaca
     2401 acgcgttgga ccatgggtac cgctggctcc tcgagcacgc cctgcgacgc cggtgggtgg
     2461 tcgtcgtcct gaccctggtg accctgttca gcatcgggcc gctcgggacc gcggtcaaca
     2521 agaacttcct gccccaggac gacgaatcgc agctcgaggt cgtcgtgcgc gcgccggaag
     2581 ggtggacgct cgacgccacc aaccggttcg cgacccaaat ggccaccgag atccggaagc
     2641 tcgcggggat cgactacacc atcgtcacga ccggcgacga ccctcagcac acccccaacc
     2701 gcttcacgat cttcgtcaag atggtcgacc tggacaagcg ctcgatcagc cagcaggcca
     2761 tggagggcaa gatccgggat ctgatcctct cccggtacgc ctacctcggg ctgcaggccc
     2821 aggtcgagca ggtatcggag ttcggcggca gcggcgggtt ccagcccgtc gactatgtcg
     2881 tgagcggccc cgacctcagt gtcctgacca gggccggcga ccaggggatc cagatcctgc
     2941 gctccatccc cggggtcgtg gacccccgct catcgcttgt cagcggcgcg ccccagttgg
     3001 ggatgcaggt ggaccgcgcc cgcgccgcgg acctgggcgt caacgcggtc gatgccgcga
     3061 acgccctgtt catgctcgtc cagggcgtcg aggtgaagac cgccaagtac gccgaggccg
     3121 gccagcagta caagatcgtc atccgcgccg acaagcagca ccggtcggac caggaagtgc
     3181 tggcccaact gcccgtctcc tccccgagcc agggcgtggt cgccctcgag caggtcgtga
     3241 ccgcaagccg gggcacggga ccgagtgaga tcacccatta caaccggcgc cgccaggtcg
     3301 agctgaccgc gaacctgctg cccggggcgt cgcagcagac cgtgttgcag gcgctcgacc
     3361 ggaaagtccg tgcgctcggc ctcggaccgc agtacgacat gacattcacc ggccaggcgg
     3421 cagagcaggg caagcaggcg gcagcgttca tgcagtcgtt catgctgtcg ttcctcttca
     3481 tgtacctggt gctggccgcg cagttcgagt cgtggatcca cccggtgacg atcctgctct
     3541 cgctgccgct gaccgtgccc ttcgctcttc tctcgatcct gatcttcggg cagtcggtca
     3601 acatcttcac gtcgctcggc atcctcgtgc tcttcggcgt cgtcaagaag aactcgattc
     3661 tgcagatcga tcacgcgaat cagctgaagg acaccggcct gtcgacgcat gacgccatcg
     3721 tccgggcgag ccgcgatcgg cttcgtccga ttctg
//
LOCUS       DASQGH010000613         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45208, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000613 DASQGH010000000
VERSION     DASQGH010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45208"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VEW91_12215"
     CDS             <1..529
                     /locus_tag="VEW91_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92386.1"
                     /translation="RVDTILMRIVDVLIAFPTLLLAILITGIFGPSLVNLVIALALTA
                     SPGTARTIRSGVVGLREREFVEATRSLGASSARILIRHVVPNVMDLLVVRATLDVSIL
                     ILAEATLSFIGIGVQPPRPSWGLMISDSFQYLGSQPQLVIIPAVALSMTVASLNLLGE
                     SLAQAFAPGHRYVFR"
     gene            550..1731
                     /locus_tag="VEW91_12220"
     CDS             550..1731
                     /locus_tag="VEW91_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM92387.1"
                     /translation="MGRLPYDLEARKPYLVPAFSAAEYDRRIRATCAAMAREGFGALV
                     VYASGAAPAAVTYLTNYVPVYGHVFAVLDGDGRITVATDGILHDEPMHSMIWTCRVAD
                     VRIARGPIYGGCADEVAALAAGAASGARRVGLVGAGALPHTLYTTLSTRIPDLHPADH
                     VLAGVRLLKSDEEIAKMREAGRIADEAYGAVFATIGPDVEETAVAAAAVHRMHVLGSR
                     EGFRTCVVSGVQAGLKHGHPRRRKMRGGEMVFIDLGAEVAGYLSDVSRCTVIGPATGA
                     ARDLLQIGLDLYHAGLRVMAPGRTIDEVSDALLAVVRGTRYEPYYCPGGFGHGIGMSL
                     LEVPGLYAGNTTELRPRMTVAYEPMVVVETLGTGVVEDTILITETGYERLSQYPVVTW
                     R"
BASE COUNT          264 a          683 c          699 g          311 t
ORIGIN      
        1 gcgggtcgac acgatcctga tgcggatcgt cgatgtcctg atcgccttcc ccacgctgct
       61 gctcgcgatc ctgatcaccg gcatcttcgg cccctcgctc gtcaacctgg tgatcgccct
      121 cgccctcacc gcgtcgccgg ggactgcgcg caccatccgc agcggggtgg tggggctgcg
      181 tgagcgcgag tttgtggagg ccacccggtc gctcggcgca tcgtcggcgc ggatcctcat
      241 ccgtcacgtc gtgcccaacg tgatggacct gctggtcgtg cgggccacgc tcgatgtgag
      301 cattctgatc ctcgccgagg cgacgctgag cttcatcggc atcggggtcc agcccccgcg
      361 cccgagctgg gggctcatga tcagcgacag ctttcagtac ctgggcagcc agccccagct
      421 cgtcatcatc cccgcggtcg ccctctccat gaccgtcgcg tcgctgaacc tcctcgggga
      481 gtcgctggcc caggcgttcg cccccggcca ccggtacgtc ttccggtgac acgcgcagag
      541 ggccagtcga tgggccggtt gccgtacgat ctcgaggctc ggaagcccta cctggttccg
      601 gcgttcagcg cggccgaata cgatcggcgc atccgcgcga cgtgcgccgc catggcccgc
      661 gaggggttcg gcgcgctggt ggtctacgcg agcggtgcgg cgccggcggc ggtgacctac
      721 ctgacgaact acgtcccggt gtatggccac gtctttgccg tcctcgacgg cgacgggcga
      781 atcaccgtgg ccacggacgg gatcctccac gacgagccca tgcattcgat gatctggacc
      841 tgtcgggtgg ccgatgtgcg gatcgcccgc ggtccgatct acggcggctg cgccgacgag
      901 gtggccgcgc tggccgccgg ggctgcatcc ggggcgcggc gcgtcgggtt ggtcggcgcg
      961 ggggcgctgc cccacacgct ctacacgacc ctctcgaccc gcatccctga tctccatccc
     1021 gccgaccacg tcctcgccgg ggtgcgcctg ctcaagagcg atgaggagat cgcgaagatg
     1081 cgcgaagccg gccggatcgc cgacgaggcc tacggggcgg tgtttgcgac gatcggtccc
     1141 gatgtcgagg agacggccgt cgcggcggcc gcggtgcacc ggatgcacgt cctgggcagc
     1201 cgcgagggct tccggacgtg tgtggtgagc ggcgtgcagg ccgggctgaa gcacggacac
     1261 ccccgccggc ggaagatgcg gggcggggag atggtgttca tcgatctggg cgccgaggtg
     1321 gcgggctacc tgtcggatgt cagccggtgc accgtcatcg ggccggccac cggggccgcc
     1381 cgggatctcc tccagatcgg gctcgacctc tatcacgcgg gcctccgtgt gatggcgccc
     1441 ggccggacga tcgacgaggt gtccgacgcg ctgctcgccg tcgtccgggg cacccggtac
     1501 gaaccgtact actgcccggg tgggttcggg cacgggatcg ggatgtcgct gctggaagtc
     1561 ccagggctct acgccggcaa cacgaccgag ctgcggccgc ggatgacggt cgcgtacgag
     1621 cccatggtgg tcgtggaaac cctggggacg ggggtggtgg aggacacgat cctcatcacc
     1681 gagaccggct acgagcgcct gtcccagtat ccggtggtga cctggcggta gccggccccg
     1741 ccggcgcatg atccgcgctg cgggctccac cgacggctca ggacggaggc gacgatgatc
     1801 ctggtacatc aacgctactt cgccaagccg gggctgcacg accaggtgct cgagacccgc
     1861 atcgaggcct cccggcgcct gcgggagctc ggcgtccccc caggacagat ctgggtcgcg
     1921 ctgagggacg agcccgggtc ccctcccgcg ggcaccc
//
LOCUS       DASQGH010000614         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_45213, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000614 DASQGH010000000
VERSION     DASQGH010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45213"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..795)
                     /gene="istB"
                     /locus_tag="VEW91_12225"
     CDS             complement(55..795)
                     /gene="istB"
                     /locus_tag="VEW91_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HYM92388.1"
                     /translation="MLSPPTMEKLHALKLAAMAAAWTAQQQDAETSALSFDERFGLLV
                     DAEWLARENARLAWALKAAKLKLSQAAVEAIDYAARRALDKALLRQLTTGRWIAEHQH
                     VLITGATGTGKTFVACALAHQACRQGYRARYWRVSRLFHACALARADGTYARLLAQLA
                     RVDVLLLDDLALQPLQDTERRDLLEILEDRYGTRSTIVTSQLPPTHWHEAIGDPTLAD
                     AICDRLLHNSHRLVLKGPSRRKEEKLEG"
     gene            complement(816..>1669)
                     /locus_tag="VEW91_12230"
     CDS             complement(816..>1669)
                     /locus_tag="VEW91_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HYM92389.1"
                     /translation="YEEFAQHMGTTILPARPAAARDKAKVEVAVQIAERWILARLRHE
                     RCFSLAALNARIAELLGELNARRMRHYDASRHELFDRLDRPALRPLPAAPFVYGEWKV
                     ARVNIDYHVDVAGHYYSVPHALVHAKVDVRVSALTVEIFHRGQRVAAHLRSQARGQHT
                     TVTAHMPKAHQRHREWSPSRIVAWAESIGPHTAQLVQAILAARPHPEQGYRSCLGLFR
                     LGRRDGPARLEAACARALAVGARSYRHVDAILKHGLDRAPLPETAPAPPPPPVVHDQV
                     RGATYYE"
BASE COUNT          229 a          550 c          630 g          260 t
ORIGIN      
        1 gaacaggtag atcgctcatc gtgaacggag cgtagcgacg ctaggcagtc tcgctcagcc
       61 ttcgagcttc tcctcctttc gccgtgaggg cccttttagc acaagccggt gactgttgtg
      121 aagcagccga tcgcagatcg catccgccag ggtcggatcc ccgatcgcct cgtgccagtg
      181 cgtcgggggc agctggctcg tcacgatggt cgagcgggtg ccgtaccgat cctcgaggat
      241 ctcgaggagg tcccgccgtt ccgtgtcttg gagtggctgg agggcgagat cgtcgagcag
      301 caagacgtcg acccgcgcca actgcgcgag gagccgggcg taggtcccat ccgcccgggc
      361 caacgcacac gcgtggaaga gccgggagac ccgccagtag cgcgcccggt atccctggcg
      421 gcaggcctgg tgggccaggg cgcaggcgac gaaggtcttc ccggtgccgg tcgcccccgt
      481 gatgagtacg tgctggtgct cggcgatcca gcggcccgtg gtgagctgcc ggagcagcgc
      541 tttgtcgagc gcgcgccggg ccgcgtagtc gatcgcctcg acggcggcct ggctgagctt
      601 cagcttggcg gccttgagcg cccaggcgag gcgggcgttc tctcgggcga gccactcggc
      661 gtccacgagg aggccgaagc gctcgtcgaa gctgagcgcg ctcgtctccg cgtcctgctg
      721 ctgggcagtc caagccgcgg ccatggcggc gagcttgagg gcgtggagct tctccatcgt
      781 cgggggactg agcatggcgt ctctcctcct ctcggtcact cgtagtaggt ggccccgcgc
      841 acctggtcgt ggacgaccgg gggtgggggt ggggctggcg cggtctccgg cagcggagcc
      901 cggtcgagcc cgtgcttcaa gatggcatcg acgtggcggt acgagcgggc gcccacggcc
      961 agggcccggg cacaggcggc ttccagccgg gctggcccat cccgccgccc gagccggaac
     1021 aggcccaagc aggaccggta gccctgctcc gggtggggcc gggccgccag gatcgcctgg
     1081 acgagctgcg cggtgtgggg gccgatactc tcggcccacg ccacgatccg ggagggactc
     1141 cactcccggt gccgctggtg ggccttcggc atgtgggcgg tgaccgtcgt gtgctggccc
     1201 cgggcctgac tgcggaggtg ggcggccacg cgctgccccc ggtggaagat ctcgacggtg
     1261 agggcgctga cgcggacgtc gaccttcgcg tgcacgagcg catgggggac cgagtagtaa
     1321 tgcccggcca cgtccacgtg gtagtcgatg ttcacgcggg cgaccttcca ctccccgtaa
     1381 acgaaggggg cggccgggag cgggcgcaag gccggccgat ccagccggtc gaacaactcg
     1441 tgccggctgg cgtcgtagtg gcgcatccgg cgggcgttca actcgccgag cagttccgcg
     1501 atccgggcgt tcagggccgc caaggagaag cagcgttcat gccggagccg ggcgaggatc
     1561 cagcgttcgg cgatctgcac ggccacctcg accttggcct tgtcccgggc cgccgccggc
     1621 cgggccggga ggatggtggt gcccatgtgt tgcgcaaact cctcgtacg
//
LOCUS       DASQGH010000615         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50875, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000615 DASQGH010000000
VERSION     DASQGH010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50875"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEW91_12235"
     CDS             <1..203
                     /locus_tag="VEW91_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92390.1"
                     /translation="LMDAARSADPTKSAEAYRKVLDVIVEDAPWLWVVHDKNAVALRK
                     RVKGLDICATPYFDLQPVWIEG"
     gene            246..1256
                     /locus_tag="VEW91_12240"
     CDS             246..1256
                     /locus_tag="VEW91_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYM92391.1"
                     /translation="MKAIVVRGVGELDALHLEDLPDPVPGPGQVVVRLRAAALNHRDL
                     FICRGQYAGLRFPIVLGSDGVGEVASIGPGVRDVRVGDPVVINPSLEWGDDPRVQGPR
                     FQILGHPDNGTFAEMVVVPVANVLPRPAGLSDEEAAAIPLAGLTAYRAVVTRGRVKRG
                     ETVLVLGIGGGVATFVLLIAKHAGARVLATSGSDAKLDRARMLGAAAAFHYKTADWVK
                     AVREATDGTGPDLIVDGTGGAPFEQALDAIRPGGRVVTYGATLGPVPQLTVRRIFWKQ
                     LDILGTTMGSPEDFRAMLALFGQGRLRPVVDRVFPLADAGAALRHMEQAAQFGKITLR
                     IA"
     gene            1384..>2164
                     /locus_tag="VEW91_12245"
     CDS             1384..>2164
                     /locus_tag="VEW91_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92392.1"
                     /translation="MQRYVLHRLLHAIPVLWGASLVVFLLVHLIPGDIVQVLLGPDAT
                     PGAVAALRGQLGLNDPIQVQYFRWLGKVLGGDLGISLQTRTPVFALLVGKFQNTLVLA
                     CAAALFATLVGMLAGIISATRQYSLFDRAAMFAALFGNSMPAFWFGLLLIMIFGTWLR
                     WFPTGGMYSVRGSGGFLDLARHMVLPTITLASVTTALIARMVRSSMLEVIRQEYILTA
                     RSKGLVERVVVLRHALRNALLPAVTVGGLQFGYLLGGAVLTE"
BASE COUNT          304 a          684 c          757 g          419 t
ORIGIN      
        1 ccctgatgga tgctgcgcgc tcggccgacc ccaccaagag cgccgaagcc tatcgcaagg
       61 tgctcgacgt gatcgtggaa gacgcaccct ggttgtgggt ggtccacgac aagaacgccg
      121 tggctctccg caagagggtc aaggggctgg atatctgcgc cacgccctac ttcgacctcc
      181 agcctgtctg gatcgagggg taggctttga cccggctgcc tgcggtcgat tgacaggagg
      241 tccgtgtgaa agccatcgtg gtgcgcggtg tcggggaact ggacgcgctc cacctcgagg
      301 atcttcccga cccggtacct ggaccgggac aggtcgtggt gcggctgcgg gccgccgcac
      361 tgaaccaccg cgacctcttc atctgccggg gccagtacgc ggggcttcgc ttcccgatcg
      421 tgctgggatc agatggggtg ggcgaggttg cgtcgatagg acccggcgtt cgcgacgttc
      481 gggtcggcga tcccgtcgtc atcaatccga gcctggagtg gggcgacgat ccgcgcgttc
      541 aggggccgcg gtttcaaatc ctgggccatc ccgacaacgg cacgttcgcc gagatggttg
      601 tcgtgccggt cgcgaacgta ttgccccgcc ccgcaggttt gtcggacgag gaggcggcag
      661 cgatcccgct ggccgggctg accgcgtatc gggccgtggt cacgcggggc cgcgtcaagc
      721 gtggagagac cgtgctggtc ctcggcatcg ggggcggcgt ggccacattt gtgctcctga
      781 ttgccaagca cgccggcgcg cgggtgctcg ccacctcggg cagcgatgcc aagttggacc
      841 gtgcgcgaat gctgggggct gccgccgcat tccactataa gacggctgac tgggtcaagg
      901 cggtgcgaga ggccaccgat gggacgggac cggatctgat cgtggacggg accggcggcg
      961 cgccgttcga acaggccctc gacgcgatcc gtccgggcgg ccgcgtggtg acctacgggg
     1021 ccactctcgg ccccgtgccg cagttgaccg tccgccggat cttttggaag cagctcgaca
     1081 tcctgggcac gacgatgggt tccccggagg actttcgcgc aatgctggcg ctctttggcc
     1141 agggacggct gcgcccggtc gtagaccggg tcttcccgct ggcagacgcc ggggccgcgc
     1201 tgcgtcatat ggagcaggcg gcgcagttcg ggaagatcac gcttcggatc gcctaaactt
     1261 cagtccgcgg ttctaccgcg gcgcggtggc ggggaacgcc tcgtcagcgg gtgagggggg
     1321 ttggacatcg ccattgcgcc ttacgttgag cttccccctc ctggatcgag ggggtagtct
     1381 cacatgcagc gctacgttct gcaccggctt ctgcacgcca tcccggtgct gtggggcgcc
     1441 tctcttgtag tgtttctgct cgtccacctc attcccggcg acatcgtcca ggtgctgctg
     1501 gggccggatg ccaccccggg ggcggtcgcc gcgctacgcg ggcagttggg gttgaatgac
     1561 cccatccagg tgcagtactt ccgatggctc ggaaaggtcc tcgggggcga tctgggaatc
     1621 tctctgcaga cgagaacccc ggtgttcgcc ctgctcgtgg gcaagttcca gaacaccttg
     1681 gtcctcgcct gcgctgccgc gttgttcgcg acccttgtcg gcatgctggc cggcattatc
     1741 tcggccacgc gccagtactc cctgttcgac cgagccgcga tgttcgccgc actgttcggg
     1801 aacagcatgc ccgccttctg gttcgggctg ctgttgatca tgatcttcgg gacctggctc
     1861 cgctggttcc cgacgggtgg catgtattcg gtgcgcggca gcggcggttt tctggacctg
     1921 gcgaggcata tggtgcttcc aaccatcaca ctggcgtccg tcaccacagc cctgatcgcc
     1981 cggatggttc ggtccagcat gctggaggtg attcgccagg agtacatcct gacggcgcga
     2041 tccaaagggc tcgtggagcg agtggtggtg ctgcgccatg cgctgcgcaa cgccctgctg
     2101 ccggcggtca ccgtgggtgg ccttcagttt ggctatctgc tgggtggcgc ggtcctgacc
     2161 gaga
//
LOCUS       DASQGH010000616         6008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2674, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000616 DASQGH010000000
VERSION     DASQGH010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1667)
                     /locus_tag="VEW91_12250"
     CDS             complement(57..1667)
                     /locus_tag="VEW91_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92393.1"
                     /translation="MRRTVLRRLVPWFLVVAMVPVGLWGVAQAQAPKTLIVAIGADQT
                     GLDPQTVQNNESGFIMATIYDSIVNYKPGSSLVGPGLAEKWEISSDGLVYTFHFRHGV
                     RFHDGTPMNARTVAQDIDRAINPQNPCYVLGRKGVDTYDDFTFGSVKDGSVVKMDVLN
                     DDTIRFTLPKPNAPFLSSLAMIWQGIVSPAATKQYNCDASQHPVGTGPFKFVEAVRND
                     HITVEADPSYWGGRPKVDRIIFQVVPESATRMLKLERNEVQILADVPPSDYPRVTGNP
                     ALKLYQQAGLTILGLAMTEDVSPFTDKRVRQAMNYAVDKDAINKGLYGGATTASQGMP
                     PVLWGYNKSVAPYPYDVARAQALLKEAGFPNGFSTEMMVYANPRGYNPVGGAKLGEAV
                     QGYLAKVGVNIKITQYEWGAYLERERHTHWEGFSIVGWSGDNGDPDNFLGDLFEYDEG
                     AGQPRLNNYARHHNPEFDRLIVQGRQVTDQAKRVQIYEQANKILHDDAPWIFMNHTNQ
                     VRAARANVQGFLLNPLQMFFHMELVSLQ"
     gene            complement(1925..1999)
                     /locus_tag="VEW91_12255"
     tRNA            complement(1925..1999)
                     /locus_tag="VEW91_12255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1965..1967),aa:Val,seq:cac)
     gene            complement(2329..2535)
                     /locus_tag="VEW91_12260"
     CDS             complement(2329..2535)
                     /locus_tag="VEW91_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYM92394.1"
                     /translation="MPVGTVKWFNAEKGYGFIVPDAGGKDLFVHKSAIQGDGYKLLTE
                     GKKVEYEETAGQKGPQATNVRVLG"
     gene            complement(2637..2867)
                     /locus_tag="VEW91_12265"
     CDS             complement(2637..2867)
                     /locus_tag="VEW91_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92395.1"
                     /translation="MWLDIVDKAQLLNLDHIESILLDRAPGDPQWTLTAVGHSGAVHA
                     ITSYKDSGEAHRALDRVRHALETIVLSSDGAR"
     gene            complement(3220..3816)
                     /locus_tag="VEW91_12270"
     CDS             complement(3220..3816)
                     /locus_tag="VEW91_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92396.1"
                     /translation="MAPARVQLYWFGFLTAAGHIGGHGTSLTLVVTLGERSSDCIEPL
                     LADLATERVRCEFCHSSIVGWQAYLRDQYLCKALVPWGIPSDLHGDDPALLGDLPKEF
                     VTPFIRGYADGNRISHRSPGSRKSSGFTLRGTSAILTRLNAIIQRHWAVSGGVVTEGR
                     DRAQLRFSDPEACRAIQSRLDTVASRVPAESAHPAPRR"
     gene            4428..4637
                     /locus_tag="VEW91_12275"
     CDS             4428..4637
                     /locus_tag="VEW91_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM92397.1"
                     /translation="MATALTMVTEAPARAIGLGTYGLRPGARADLVVLDLRGPAGPEH
                     VLSRQPAGISCCAAASPRSRPPDCG"
     gene            complement(4705..5739)
                     /locus_tag="VEW91_12280"
     CDS             complement(4705..5739)
                     /locus_tag="VEW91_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYM92398.1"
                     /translation="MARLQAEAAESAIAPLERALDLTVQQGEEVLQARIELALAMVDQ
                     TLGLLPRAQRRMDRCLRVLVRAGDAASLAAAHCCLGLIKLHSGDPASAIWAFQAGLQF
                     AERLPQDPALRSRLYFEIGMAHRRLGHIQAARDSFGAALEAAAPFRDHHRVASWHLHL
                     ADAAVEGGRYEQGCEHAGNALAIHEALAHTRRAAEIHECLGGLDREEGRWEEAACHYR
                     WSVVLNGAAANLPGSAQTLARLAEALLNRASPEAARAMCETALDLLGGDPGRDERADV
                     LRILGTIYRMAGQRAEAKTALQESLDLLSRCNRKDDARLVRQELALLALEAGDSEEAR
                     RHLETLRDTP"
BASE COUNT          919 a         2081 c         1957 g         1051 t
ORIGIN      
        1 cgggggcctg cccctcgagt ggggcaggcc cccgggtgtt ccggcggtcg gcccggctac
       61 tgcagcgaca ccagctccat atggaagaac atctggagcg ggttgagcag aaagccctgc
      121 acattcgcgc gtgccgcccg gacctggttc gtgtggttca tgaagatcca gggcgcgtcg
      181 tcgtgaagga tcttgttagc ctgctcatag atctggacgc gcttggcctg gtcggtgacc
      241 tggcggccct ggacgattag cctgtcgaac tcagggttgt ggtggcgagc gtagttattg
      301 agacgcggct ggccagcgcc ctcatcgtac tcgaacaggt cgccaaggaa gttgtccggg
      361 tcgccgttgt cgcccgacca gccgacgatc gagaaccctt cccagtgcgt gtgccgctcc
      421 ctttccaagt acgcacccca ctcgtactgg gtgatcttga tgttcacgcc gaccttggcc
      481 aagtacccct ggaccgcctc cccgagcttc gcaccgccca cggggttgta gcccctcggg
      541 ttggcataga ccatcatttc ggtcgaaaat ccgttgggga acccggcctc cttgaggagg
      601 gcctgtgccc tggccacgtc gtacggatac ggggccaccg acttattgta tccccacagc
      661 acgggcggca tgccctggct ggctgtcgtc gcgccgccgt agagtccctt gttgatcgcg
      721 tctttgtcga cggcgtagtt catcgcctgg cgcacccgct tgtctgtaaa cgggctgacg
      781 tcctcggtca tcgctaggcc gaggatcgtg agccccgcct gctggtagag cttcagcgcg
      841 gggttgccgg tcacccgggg atagtccgag ggcgggacgt cagccaggat ctgcacctcg
      901 ttccgctcca gcttgagcat ccgggtggcg ctttcgggca cgacctggaa gatgatccgg
      961 tcgaccttcg ggcgaccgcc ccagtaggac gggtcggctt ccacggtgat gtggtcgttc
     1021 cggacggcct cgacgaactt gaacggcccg gtccccaccg gatgctggct cgcgtcgcag
     1081 ttgtactgct tcgtggccgc cgggctcacg atcccctgcc agatcatcgc gaggctgcta
     1141 aggaacggtg cgttcggctt cggaagcgtg aaccggatcg tatcgtcgtt cagcacgtcc
     1201 atcttcacga cgctaccgtc cttgaccgag ccgaatgtga aatcgtcgta ggtgtcgacg
     1261 cccttccgcc ccaacacgta gcagggattt tgggggttga tcgcgcggtc gatatcctgc
     1321 gcgaccgtcc gcgcgttcat cggcgtgccg tcgtggaacc ggacgccgtg tcggaagtga
     1381 aacgtgtaca ccagaccgtc cgacgagatc tcccacttct ccgcgagccc ggggccgacc
     1441 aggctactgc cgggtttgta gttgacgatc gaatcgtaga tcgtcgccat gatgaatccg
     1501 gactcgttgt tctgcacggt ctgcgggtcc aaccccgtct ggtccgcccc aatcgcgacg
     1561 ataagcgtct ttggcgcctg cgcctgggcc acaccccaca gccccaccgg aaccatcgca
     1621 accaccagga accacggcac gagtcgccgc aacactgtgc gcctcatcct cttcctcctc
     1681 tcgcgacacg tacgaacagg acagaactcc tcaagcagat tgcctgcgcg aacgatacta
     1741 agaaacaccc ccggagcgca ctgaccctcc cccccgcctg agccccggct ccgggccggc
     1801 gcggcgcgga gctcccgggc tcggtgcatg gatcgagatc acgcggtgca atggccggag
     1861 ggattgagcc ccaggattga tgcgggtcct agcgatcggg cgcaatttgg tgccacaggg
     1921 cgcatggtgg gcggtgcagg ttttgaacct gcgacctcat ccgtgtgaag gatgcgctct
     1981 gccactgagc taaccgcccg cttccggaga cgccggcttc ttcgtggcaa aatttggaac
     2041 cggtagagcc tggactctgg gggtctccta ccgccgcttc ccccagcctt tcgaggccgc
     2101 ggggatggct ccagcggcag gagaccgctc gcgcccgccg gggctccgac gctcccccgt
     2161 cgcggtgccg tagtatcgcc tcgcgcactc ggtcgagcag aagtcatccc cctgctcgac
     2221 ggcgagcgcg ggcctgaagt tgccacaacc cttgcatggc tcggggcgca tcgctacact
     2281 cccgtacaca cgagcttgat tgtcgcgccc aacgtgagcg acgtaagact agcccaatac
     2341 gcgaacgttt gtggcctgcg gtcccttctg cccggcggtc tcctcgtact cgaccttctt
     2401 gccttcggtc agcaacttgt atccgtcacc ctggatcgca ctcttgtgca cgaacagatc
     2461 cttgccgccc gcatcgggca cgatgaaccc atagcccttt tccgcgttga accacttgac
     2521 cgtccctact ggcatgctcc acctcttcgt ctttccaagc tgctgatgcc acttaagtat
     2581 gacacaggat gcacgccaaa gcaaagaacg gccgcgccgg gacatcgcgc ttccgactat
     2641 cgcgccccgt cgctcgagag cacgatcgtt tccagggcat gcctgacgcg gtctagcgcc
     2701 ctgtgggcct ctccggagtc cttgtagctg gtgatggcgt gcaccgcccc gctatggcca
     2761 accgcggtca gcgtccactg cgggtccccg ggtgcacgat ccagcaagat gctctcgatg
     2821 tggtcgaggt tgagaagctg tgctttgtcc acgatgtcca gccacatctc gtcccgcccc
     2881 cctctgtcgc gcccgactca cccgtgctcc caccctcgcg ccgttggacc ccgctgcgac
     2941 gtcctgctag agtgaaagtc aggcggcatc gcggggtggc ctatgtcgaa gcctgtgcgc
     3001 gcgaaggccg gactgccatg gcctgtgacc ggcgtctacc gctgcgacaa atgccacacc
     3061 cggagggaag gcgaggccgg agtcattgcg acgcgctgta ccgttctcct cggcctgcat
     3121 cagcgacagt gcaacggtgc ctatttcgtc atgatcgagc cccctgctcc tgatgagcgc
     3181 cccgtcccgg ctcggtgagg gccggccgga ggcggcgggc tagcgccgtg gggcggggtg
     3241 tgcgctctcc gccgggaccc gggaggccac cgtgtccaac cggctctgaa tggcgcggca
     3301 ggcttccggg tccgagaagc ggagttgggc ccggtcccgc ccctcggtca cgacgccgcc
     3361 tgaaaccgcc caatgccgct ggatgatcgc gttgaggcga gtaaggatcg ccgacgttcc
     3421 ccggagggtg aagccgctgc tcttccggct gccgggggat cggtgcgaga tcctgttccc
     3481 gtccgcgtag ccgcgaatga atggggtgac gaactccttc ggcagatccc cgagcaatgc
     3541 cgggtcatcc ccgtgcagat ccgacgggat gccccacggg accagggcct tgcacaggta
     3601 ctggtcgcgc agataggctt gccacccgac gatgctgctg tgacagaact cgcaccggac
     3661 acgctccgtc gcgaggtcag cgagcagggg ctcgatgcag tcgctcgatc gctcgccgag
     3721 cgtcaccacg agggtcagcg aggtgccgtg ccccccgatg tggcccgccg ccgtgaggaa
     3781 cccgaaccag tacagttgca cacgcgcggg ggccatgccc ggcccctccc ggcgggtccg
     3841 aatgaggtct accggatccg acaaacgcgg atgctttgcg ggagcgagca cccgctccac
     3901 cgctgcgacg gggagttggt acacgtcggc gatttcggtc gagttccggc cctggcgggc
     3961 ggccgcccga atctgttggg ttcgctcccg ccagtcgaga tcaggcccac tcgggcctcg
     4021 cattcgcaag gggccctagc gaccccggcg acccaaggaa tcgaggcccc gggtggtcat
     4081 gaccgccatt cctgatccgt cctggggggg gagcccggcg agtccggagt cggcgcaaca
     4141 tcgcctggag cgggcggggg tgtagccccc tccgatgccg acggggtcgt ccgggcgtgt
     4201 tttcgggcca gcttggcttc gcgcttcgct ttctgcttct gctcccgggc gcgcttggcg
     4261 gcggtgtagc gggggcgggc catccgtcac gctcctctcc gatcgaatac attaagtata
     4321 ccacaccgcg gatcgccgaa cggggttgga ccgacgcgca cgtagtcgtt ccccggcagc
     4381 gtccggcgct cacctgctcg gtagcgcacc tgcccggccc tgcgggcatg gcgaccgccc
     4441 tcacgatggt gacggaggcc ccggccaggg cgatcggcct cggcacctac ggtctccgcc
     4501 cgggggcgcg ggccgacctc gtcgtcctgg atcttcgggg gcccgccggg ccggagcacg
     4561 tcctcagccg gcagcccgcc ggcatctcgt gctgtgcggc ggcatcccct cggtctcgac
     4621 ccccggactg cgggtgacct gagcccgtca gtagggccgc tcgacagttc cccggcgcgg
     4681 atcggcggcc gagcggcgtt gtcgctaggg cgtgtcgcgc aacgtctcga ggtgccgtcg
     4741 agcttcctcc gagtctccgg cttcgagcgc caggagcgcc agctcctgac gcacgaggcg
     4801 ggcatcgtct ttgcggttgc accggctcag cagatcgaga ctctcctgca acgcggtctt
     4861 ggcctctgcg cgctgcccgg ccatccggta gatcgtcccg aggatccgga gcacatcggc
     4921 ccgctcgtcc cggccggggt ctcccccgag caggtctaac gccgtctcgc acatcgcgcg
     4981 ggccgcctcc ggggaggcgc ggttgaggag cgcctccgcc agcctggcca acgtctgcgc
     5041 cgatcccggg aggttggcgg cggccccgtt cagtacgacg ctccaccggt aatggcacgc
     5101 ggcctcttcc cagcgtccct cctcgcgatc gagcccgccg agacactcgt ggatctctgc
     5161 cgcgcgccgc gtatgcgcca gggcctcgtg gatggccagc gcgttgccgg cgtgctcaca
     5221 cccctgttca taccgcccac cctccaccgc cgcgtcggcc aggtggagat gccaggatgc
     5281 cacgcggtga tgatcccgaa agggcgccgc cgcctccaag gccgcgccga aggagtcacg
     5341 ggctgcctgg atgtgaccca gcctgcggtg ggccatgccg atctcaaagt acaatctcga
     5401 ccggagcgcc gggtcctggg ggagccgctc cgcgaactgc aggcccgcct gaaacgccca
     5461 gatggcggag gccggatctc cggagtgcag cttgatgagg cccaggcagc agtgggcggc
     5521 ggccagcgac gcggcgtcgc cggcgcgaac cagcacgcgc aggcagcggt ccatccggcg
     5581 ctgggcgcgc gggaggagcc ccagggtctg gtccaccatc gcgagcgcca gttcgatccg
     5641 ggcctgcagc acctcctcac cctgctgcac cgtcaggtcc agcgcccgct ccaacggcgc
     5701 gatcgccgac tcggccgcct cggcttggag acgggccaac ccgagacgca ggtaggcctc
     5761 cgcttctcga tcctccggga cgccgtcgag gaagtgtgcg agcggcctct cgagccgccg
     5821 cgccaggacc ttgagggtga tgagccgcgg gatgcggcgg ccgcgttcaa cttcgctgat
     5881 gtaggacttc gtgaggtcat gggcgcaaag gagaaaaccc tgtagagact aggagatggc
     5941 ccacccatgc gaggcacgcc gggtgctccg acgttggccc aggaccccgt aggcatatgg
     6001 taagattg
//
LOCUS       DASQGH010000617         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53535, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000617 DASQGH010000000
VERSION     DASQGH010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53535"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VEW91_12285"
     CDS             <1..995
                     /locus_tag="VEW91_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYM92399.1"
                     /translation="LDDGSDATRSRSLVEKLVTDQHVNFLLGGYDTSLVEAQEVVPDQ
                     YKIPYVEGGGAASEIFKRGYKYVFGTLSHVYNMGTITMEFLAYEISQHKLPKPVTIAV
                     VWENTDHGKDYVSAVLDQAKSLHPDLFQIVLNQPFQLNGSDFSPLLQQVKASNANVFL
                     SDAHLPDFITMHRQYLQAGLYHQFVSYGARGPDQRGRQALGSGADYLVAALWWTPALK
                     DPASQLFTERYTKEYHAVPDWFQALSYDTARVLLQAIQDAGSLSGPAVRDALSRVVFR
                     KSLLPGGVIRFEANGQVIAPYVMVETMPGNTIRIVWPQRLPETRDAILPMPKAQ"
     gene            1012..>1707
                     /locus_tag="VEW91_12290"
     CDS             1012..>1707
                     /locus_tag="VEW91_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92400.1"
                     /translation="MHYSVQDLVSVGIAAVLTAGLYAIMSYGLAIIYGVMRVINLSHA
                     GFLMLGAFMTLVYFQQWRLDPVAGAFVNLPVFFVLGWLVYKVLVRRVAAALPLASLLL
                     LFALWLVIQNVALAIWGPDDQSIITAYTYKALSFGGFKVPVTRLIVFGVAFGVLSIMH
                     FVLGHTHLGKALRATVQDPQAALLVGIDTARISAVAFGIGTALAAFAGSLLTLLFSFT
                     PSFGDSFQLKSFTI"
BASE COUNT          275 a          574 c          533 g          325 t
ORIGIN      
        1 tcctcgacga cggcagcgac gccacccggt cccgcagcct cgtcgagaag ctcgtgaccg
       61 accaacacgt gaacttcctg ctcggcgggt acgacacgtc cctcgtcgag gcgcaagagg
      121 tcgtcccgga ccagtacaag atcccgtacg tggagggcgg gggggccgct tcggagatct
      181 tcaagcgggg gtataagtac gtcttcggta ccctgtcgca cgtttacaat atggggacca
      241 tcacgatgga gttcctcgcg tatgagatca gccagcacaa gctcccgaaa ccggtcacga
      301 tcgccgtcgt gtgggagaac accgaccacg gcaaggacta tgtcagcgcg gtcctcgacc
      361 aggcgaaatc gctgcacccc gacctgttcc agatcgtcct caaccagccg ttccagctca
      421 acgggagcga cttcagcccg ctcctgcagc aggtgaaggc gtcgaacgcg aacgtgttcc
      481 tctcggacgc ccacctgccg gatttcatca cgatgcaccg gcagtatctg caggccggcc
      541 tgtaccacca gttcgtgtcg tacggcgcgc gcggtcccga tcagcgaggg cggcaggcac
      601 tcggatccgg cgccgactac ctcgtcgcgg cgctgtggtg gactccggcg ctcaaggatc
      661 cggcctccca gctcttcacg gagcggtata cgaaggagta ccacgccgtg cccgactggt
      721 tccaggcgct ctcctacgac acggcccggg tgttgctcca ggcgatccag gacgccggca
      781 gcctctccgg tccggcggtg cgggatgccc tctcccgcgt ggtcttccgc aagtccctgc
      841 tccccggcgg cgtgatccgg ttcgaggcaa acgggcaggt catcgcgcca tacgtcatgg
      901 ttgaaacgat gcccgggaac accatccgga tcgtctggcc gcagcggctg ccggagacgc
      961 gagacgccat tctgccgatg ccgaaggccc agtaggaggg acgggacggg ggtgcactac
     1021 agcgtccagg acctcgtctc ggtcggcatc gcggctgttc taacggcggg gctgtacgcg
     1081 atcatgtcgt acggcctcgc catcatttac ggcgtgatga gggtcatcaa cctcagccac
     1141 gccggattcc tcatgttggg cgcgttcatg acgctcgtgt acttccagca gtggcggctc
     1201 gatcccgtgg ccggagcgtt tgtgaacctc cccgtgttct tcgtcctcgg ctggctcgtg
     1261 tacaaggtgc tggtccggcg cgttgcggcg gcgctgccac tcgcctccct cctcctgctg
     1321 tttgcgctct ggctcgtgat tcagaacgtc gccctggcga tctggggacc cgatgaccag
     1381 tcgatcatca ccgcctacac gtacaaggcg ctgtcctttg ggggcttcaa ggtcccggtc
     1441 acgcggctta tcgtcttcgg ggtcgcgttc ggtgtcctca gcatcatgca ctttgtgctc
     1501 gggcacaccc atctcgggaa ggccctccgg gctacggtgc aggatccgca ggccgcgttg
     1561 ctcgtcggga tcgacacggc acggatcagc gcggtggcgt tcggaatcgg cacggcgctg
     1621 gccgcgttcg ccgggagcct gctcaccctc ctcttcagct tcacgccgag cttcggcgat
     1681 tcatttcagc tcaagagctt cacgatc
//
LOCUS       DASQGH010000618         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_56276, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000618 DASQGH010000000
VERSION     DASQGH010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56276"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..638
                     /locus_tag="VEW91_12295"
     CDS             24..638
                     /locus_tag="VEW91_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYM92401.1"
                     /translation="MSGMRGRLFLTLILPGFAGIFALAVGAGYLMGQTAFRPSVPRPG
                     PTMPTTLTPGLSTPSSAEPQPQPSPLSPDPLIVGEQPGGALLPQTEPPASPEPAQPPA
                     VDVQPAPAVSAPPPVMAPGGGPTTSPPIPEPPSRFHVQVGAFDDRGNAGAMVDRLRAR
                     GYAVTLVEGPPYRVWVGGYLDRSTAERLSAHLKAAGFEATLSPR"
     gene            750..1523
                     /locus_tag="VEW91_12300"
     CDS             750..1523
                     /locus_tag="VEW91_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM92402.1"
                     /translation="MPYEHIIVTTGDGIGTVTLNRPRVLNALNQALMEELADAMETLD
                     RDDAVRCLVLTGNDRAFAAGADVHEFADATPADMVRGYRFQQWERIRRVGKPVIAAVS
                     GYALGGGCELAMACDIVIASETARFGQPEIRLGLIPGAGGTQRLTRAIGKSRAMELVL
                     TGRYLTAAEALGLGLVSRVVPVELYLEEAKALAREIAGQPPIAVRMAKEAVLQAFETT
                     LGGGLEFERRCFHLLFGTEDKREGIRAFLEKRPAQFTGR"
     gene            1520..2224
                     /locus_tag="VEW91_12305"
     CDS             1520..2224
                     /locus_tag="VEW91_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYM92403.1"
                     /translation="MRRRDSLGALARDVVACTACPRLRRYCARVAAQGKREFAGWAYW
                     GRPVPGFGDPRARLLIVGLAPAAHGANRTGRMFTGDGSGAWLARAMHRAEFANQPTSL
                     RRDDGLRLRNAYITAAVRCAPPDNTPTAREMARCSAFLEREAALLPRVRVVLALGQVA
                     FGACCRVLGARGAPVRGLHFAHGTVHALGPAFPTVVCSYHPSRQNTNTGRLTEAMLDR
                     VFATVRELLEGARTAR"
     gene            2237..3013
                     /locus_tag="VEW91_12310"
     CDS             2237..3013
                     /locus_tag="VEW91_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYM92404.1"
                     /translation="MYETLLAETKDGVRVITLNRPEVLNAVNAQMGRELLDALRDAER
                     DAGARCVLLTASGRGFCAGADLREQGSGQTSVGDLLRTRYNGIVLRLRTMEKPVIAAI
                     NGVAAGAGCNLALAADLRIASERASFIEVFSRVGLIPDSGGTWLLPRLVGVGRALEMA
                     FFADPVDAATAERVGLVNRVVPHDDLMPRATEWAVRLAAGPTRAYGLIKRGVNQALAT
                     SFSEALEYEARLQEIAGRTEDHREGVAAFLAKRPPVYTGR"
     gene            3041..>3664
                     /locus_tag="VEW91_12315"
     CDS             3041..>3664
                     /locus_tag="VEW91_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYM92405.1"
                     /translation="MRDVVILDAVRTPVGRYAGALRSVRPDDLAAHVIQAIVRRHTLD
                     PGVIEDVVMGCANQAGEDNRNVARMAALLAGLPVEVPGQTVNRLCGSGMQAVHTAAHA
                     IACGLGDVYIAGGVESMTRAPYVLGSPEEAFPRGPMVLQDTTIGWRFPNPKLAEIYPL
                     YSMGETAENVAERYGISRDDQDLFAWESQQRAALAVREGRFAEEIVPV"
BASE COUNT          513 a         1268 c         1353 g          530 t
ORIGIN      
        1 cgaacacagt gggggatgga ggcatgagcg gtatgcgcgg gcgcctgttc ctgaccctca
       61 ttcttccggg gtttgcggga atcttcgcgc tggcggtcgg cgccgggtac ctgatggggc
      121 agacggcctt ccggccgtcc gtgccccggc ccgggcccac catgccgacc acgctcacac
      181 ccgggttgtc gaccccgtcg agcgccgagc cacagcccca gccttcgccg ttgtcccccg
      241 acccgctgat cgtgggagag caacccggtg gcgcgctgct gccgcagacg gagcccccgg
      301 cgtcccccga gccggcgcag cctccggcag tcgacgtcca acccgcgccc gcggtatcgg
      361 cgcctccgcc cgtgatggcc ccaggaggcg gccccaccac ctcccccccg attcccgagc
      421 cgccgtctcg gtttcacgtg caggtcggcg cgttcgacga ccgggggaat gcaggggcga
      481 tggtggaccg gctgcgcgcc cgcggctacg ccgtgaccct tgtcgaagga ccgccgtacc
      541 gggtctgggt gggcggctac ctggaccgat ccactgccga gcgcctctcc gcccacctca
      601 aagctgccgg gtttgaagcg accctgagcc cgcgctaggc tgcggctttc ccggtctgtc
      661 cgcccgcctc ccccggatcc gtccgcaaca gggggacggg aaggggcgga gagaagaaga
      721 ggaccacgcc ttcctcgggg gagccgccca tgccctatga gcacatcatc gtcaccaccg
      781 gtgacggaat cggcacggtc acgctgaacc ggccgcgagt cctcaacgcg ctcaaccagg
      841 cgctcatgga ggaactggcg gacgcgatgg agaccctgga ccgagatgac gccgtccggt
      901 gcctcgtgct gacggggaac gaccgcgcgt ttgcggccgg agccgacgtg cacgagttcg
      961 ccgatgcgac cccggccgac atggtgcggg ggtaccggtt ccagcagtgg gagcggatcc
     1021 ggcgggtcgg caagcccgtg atcgcggccg tgagcgggta cgcgctcggc ggtgggtgcg
     1081 aactcgccat ggcctgcgac atcgtcatcg cctccgagac ggcccggttt ggccagcccg
     1141 agatccgcct cggcctgatt cccggagccg ggggaaccca gcgcctcacc cgggccatcg
     1201 ggaaatcccg agccatggaa ctggtcctca ccggtcgata cctcaccgcc gcggaggcct
     1261 tgggcctcgg gctggtctct cgggtcgtgc ccgtcgagtt atatctcgag gaagccaagg
     1321 ccctcgcccg agagatcgcc gggcagcccc cgatcgccgt gcgcatggcc aaggaggcgg
     1381 tgctccaggc gttcgaaacg acgctcgggg gcgggctcga gttcgagcgc cggtgcttcc
     1441 acctgctgtt cggcaccgag gacaagcgcg aggggatccg cgcgttcctc gagaagcggc
     1501 cggcccagtt cacgggacga tgaggcggcg cgattcgctg ggggcgctgg cgcgcgacgt
     1561 cgtcgcgtgt accgcctgcc cacggcttcg ccgctactgt gcccgcgtcg ccgcgcaggg
     1621 gaagcgcgag tttgccggat gggcgtactg ggggaggccc gtcccggggt tcggggatcc
     1681 gcgcgcgcgg ctgctgatcg tcgggctcgc cccggccgcc catggcgcca accggaccgg
     1741 gcggatgttc acgggggacg gatccggagc gtggctggcc cgggcgatgc atcgcgcgga
     1801 gttcgccaac cagccgacct ccctccggcg ggacgacggc ctgcgcctcc gcaacgccta
     1861 catcaccgcg gcggtgcgct gcgcgccgcc ggacaacacc cccacggctc gggagatggc
     1921 ccggtgctcg gcgtttctcg agcgggaggc cgcgctgctc cccagggttc gcgtggtcct
     1981 ggcgctcgga caagtcgcct tcggcgcgtg ctgccgggtg ctgggggcgc gcggcgcgcc
     2041 ggtgcggggc ctgcatttcg cccacggcac cgtccacgcg ctcgggccgg cctttcccac
     2101 ggtggtgtgc tcctaccatc ccagccggca gaataccaac accggacggt tgaccgaggc
     2161 gatgctggac cgcgtctttg cgacggtccg ggaactgctc gagggcgcgc gtacggcgcg
     2221 ctgagggggg tgggccatgt acgagaccct gttggctgag accaaggacg gcgttcgggt
     2281 catcacgctg aaccggccgg aggtgctcaa cgcggtcaac gcgcagatgg ggcgcgagct
     2341 cctggacgcg ctgcgcgatg cggagcgcga cgcgggggcg cggtgcgtgc tgctgaccgc
     2401 ctccggccgc ggattctgcg ccggggcgga tctccgggag caggggagcg ggcagacctc
     2461 ggtgggagac ctgctccgga cgcggtacaa tgggatcgtg ctgcgcctca ggacgatgga
     2521 gaagccggta atcgccgcga tcaacggcgt cgccgcgggg gcagggtgta acctggcgct
     2581 ggccgccgac ctgcggatcg cgtccgagcg ggcgtcgttc atcgaggtgt tttcccgggt
     2641 cgggctgatc cccgattccg gcgggacgtg gctcctcccg cgacttgtcg gcgtggggcg
     2701 ggcgctggag atggcgtttt tcgccgatcc cgtcgacgcc gcgacggcgg agcgggtggg
     2761 gctcgtgaac cgtgtcgtcc cgcacgacga cctgatgccg cgcgccaccg agtgggcggt
     2821 ccgcctggcc gccggtccga cccgggcgta cggactcatc aagcggggag tgaaccaggc
     2881 cctcgccaca agtttcagcg aggccttgga gtacgaagcc cggcttcagg agatcgccgg
     2941 caggaccgag gatcaccggg agggcgtggc ggcattcttg gccaagcgcc ctccggtgta
     3001 caccggccgt tagcccggga gacgacaggg ggttccaccg atgcgggacg tggtgatcct
     3061 ggacgcggtc aggacgcccg tcggacgata cgcaggcgcg ctccgcagcg tccggcccga
     3121 cgacctcgcc gcgcacgtca ttcaggcgat cgtccggcgg cacacgctcg atccgggcgt
     3181 catcgaggac gtcgtgatgg gctgcgccaa ccaggcgggg gaggacaacc gcaacgtggc
     3241 ccggatggcc gccctgctcg ccggactccc ggtcgaggtg cccgggcaga cggtgaaccg
     3301 gctctgcggc tcggggatgc aggcggtcca caccgccgcg cacgcgatcg cctgcggcct
     3361 gggcgatgtc tacatcgccg gcggggtcga gagcatgacc cgcgccccgt acgtgctcgg
     3421 atcacccgag gaagcgtttc cccgggggcc gatggtcctg caggatacga cgatcgggtg
     3481 gaggttcccc aaccccaagc tggccgagat ctatcccctc tacagcatgg gcgagaccgc
     3541 cgaaaacgtc gccgagcggt acggcatctc gcgcgacgac caggatctgt ttgcctggga
     3601 gagccagcag cgcgcggctt tggcggtgcg ggagggacgg tttgccgagg agatcgtgcc
     3661 ggtc
//
LOCUS       DASQGH010000619         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2697, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000619 DASQGH010000000
VERSION     DASQGH010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VEW91_12320"
     CDS             complement(<1..1173)
                     /locus_tag="VEW91_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HYM92406.1"
                     /translation="MSAAEPAATATTGPRPHGGSTARVLGVIPLTARVELTGAPLVRR
                     LLRSRWSTFIPTPVHLFLFLIILLAGIVGTPVGNANIAIVFIWILWWSALMLVLVPFG
                     SRIWCGICPLPVVGEWLQRGTFVQRAGKLRGLAMKWPKPLRNMWFVNGVFVGVAVFSG
                     IITTRPWATVALLGTIMVLSTGLFLVYEKRTFCRYLCPVGGFLGLYANFSTIEIRSKN
                     MPVCVGHKSKECILGSTSGYGCPWLEQPTNLKRNTYCGMCFECFRSCTFDNMGVYLRL
                     PGTDLLVDSHRGLDEAWKAFIMLGAAAVYSATMMGPWGTLKDWANVRSFGGWGVFAAG
                     LIALLLVGLPALHGAFAWISWRLGGRAVPFKRVFTNFAYTLVPLGLLAWIGFSFAIL"
     gene            complement(1170..1628)
                     /locus_tag="VEW91_12325"
     CDS             complement(1170..1628)
                     /locus_tag="VEW91_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYM92407.1"
                     /translation="MRRRSLVLGLTIVLVIGVGLTLRLAASPVRAHPREITLQASQWR
                     YSPGIITVNQGDQVTVILKAEDVVHGFYLDGYGISRAVRPDQTAEIHFVASKPGRWMF
                     RCSVTCGPFHPYMIGWLRVEPNTTLKFGWIVVGAVGVAALLFAWEEARKP"
     gene            2302..>2754
                     /locus_tag="VEW91_12330"
     CDS             2302..>2754
                     /locus_tag="VEW91_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92408.1"
                     /translation="MYPRDDAPGSGRRGRFVSLQARITLSVGVAVAVILVAFGYSATW
                     AIRESTSTVLQDRVQIAEVLAGQIATRLTGDRDTLEDLARTLSQDPDRRREEAVLQQV
                     AESRRFSGLALGKPGGRLLWLPPALWPTPSIPALSPVLRAAPQAGRPDP"
BASE COUNT          483 a          945 c          875 g          451 t
ORIGIN      
        1 gagaatcgcg aagctgaacc cgatccacgc cagcaggccg agcgggacca gcgtgtaggc
       61 gaagttcgtg aacacccgct tgaacgggac ggcgcggccg ccgaggcgcc acgagatcca
      121 cgcgaacgcg ccgtgcagcg ccgggaggcc gacgaggagc agggcgatga gccccgcggc
      181 gaacacgccc caccctccga aggaccggac gttcgcccag tccttgaggg tcccccacgg
      241 ccccatcatc gtcgcggaat agacggccgc ggcgccgagc atgatgaagg ccttccaggc
      301 ctcgtcgagt ccccggtggg agtccaccag gagatcggtc cccggcagcc gcaggtacac
      361 ccccatgttg tcgaaggtgc aggaccggaa gcactcgaag cacattccac agtaggtgtt
      421 gcgcttcaga ttcgtgggtt gttcgagcca ggggcaaccg taccccgagg tgctgccgag
      481 gatgcactcc ttggacttgt gccccacgca caccggcatg ttcttcgacc ggatctcgat
      541 cgtcgagaag ttcgcgtaca ggccgaggaa gcctccgacg gggcagaggt accggcagaa
      601 cgtccgcttc tcgtacacga ggaacagtcc cgtggacagg accatgatgg tcccgagcag
      661 cgccaccgtg gcccacgggc gggtcgtgat gatgccgctg aacacggcca cgcccacgaa
      721 cacgccgttg acgaaccaca tgttgcggag cggcttgggc cacttcatgg cgagaccccg
      781 cagtttgccg gcgcgctgca cgaacgtgcc ccgctggagc cactcgccca ccaccgggag
      841 cgggcagatc ccgcaccaga tccgtgatcc aaacgggacc aggaccagca tgagggccga
      901 ccaccacagg atccagatga agacgatcgc gatgttcgcg ttgccgaccg gggtgcccac
      961 gatgccggcc agcaggatga tcaggaagag gaagaggtgc acgggggtgg ggatgaacgt
     1021 gctccaccgg ctccgcagga gtcgccgcac gagcggcgct cccgtcagct cgacccgcgc
     1081 cgtgaggggg atcaccccca gaacgcgggc ggtggacccg ccgtgaggac gcggtcccgt
     1141 cgtggcggtc gcggccggct ctgctgcgct catggcttcc gggcctcctc ccacgcgaag
     1201 agcagcgcgg cgacgccgac cgcgccgacg acgatccacc cgaacttcag ggtcgtgttg
     1261 ggctcgacgc gcagccagcc gatcatatac ggatggaacg ggccgcacgt caccgagcac
     1321 cggaacatcc agcgcccggg cttcgaggcg acgaagtgaa tctcggccgt ctgatccggg
     1381 cgcaccgccc tcgagatccc gtacccgtcc aggtagaacc cgtggaccac gtcttcggcc
     1441 ttgaggatca cggtcacctg gtctccctgg ttcaccgtga tgatcccggg cgagtagcgc
     1501 cactggctcg cctggagcgt aatctcccgc ggatgcgcgc ggaccggcga tgcagccagg
     1561 cggagggtca gccccactcc gatcaccagc acaatggtca gtcccagcac gagcgatcgt
     1621 cgccgcatcg ttcaatcctc ccttgacagt cccacatcga tcacgagtgc cgcgggagcg
     1681 tcgatgtctc cggggatcgt caccgtggcc cctgccgcct gggcgggagt gcccgggagc
     1741 cataggcttg atcccggccc agctgctcgt cccatggcgc tcgctgcgat cgtcgggcat
     1801 tcacaccgca tgccgcgacg atcccgctat cccgcctgcg ccgccttgga gaaggctttc
     1861 tccacgggcg cgtcctgctc tgtaggagag tcctctgttg cggcctcacc ccagtagcag
     1921 tagtcgcaga acttaagcaa gatacccttc ctctcgagct cgtgccgcac ggcccgcagt
     1981 ccgcactttt cacacaccgg aagcaattcc atgccctctc acccctcgcg caaaccggat
     2041 atgccgttat gtaggtccct ccctcaaagt tgcgcagatc tctctcacct cggagcatag
     2101 accgtggcgg ggcgccgccg catccccgaa cgatcccatt tctcagtggt gtctttccgt
     2161 gatgggagag atggtgtctt tccgtagggg gtatcggttc tcgacccgat ggccgctgta
     2221 gatgcgcacg ttctatcctt atacttaaga tatacttaag atacttaagg attttgcttg
     2281 ttggcctgca gggaggcagc gatgtaccca agggacgacg ccccagggtc tggccgccgg
     2341 ggccggtttg tgagcctcca ggcgcgcatc acgctcagcg tgggcgtggc ggtcgccgtt
     2401 attctggtcg ccttcggata cagtgcgacc tgggcgatcc gggaaagcac atccacggtg
     2461 cttcaagacc gcgtccagat cgctgaggtt cttgcggggc agattgccac ccgactcacc
     2521 ggcgaccgcg acaccctgga ggatctggcg cgaacgctgt cccaggatcc agatcgtcgg
     2581 cgggaggagg ccgttctgca acaggtcgcc gagagccgca ggttctccgg cctcgcgctg
     2641 gggaagccgg ggggtcggct cctctggttg ccccccgctc tgtggcccac gccctccatc
     2701 cccgccctgt ctcccgtgct tcgcgctgcc ccccaggcag ggcgtcctga tcct
//
LOCUS       DASQGH010000620         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36722, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000620 DASQGH010000000
VERSION     DASQGH010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36722"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VEW91_12335"
     CDS             <1..478
                     /locus_tag="VEW91_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92409.1"
                     /translation="LRAKGDIRMPNVVESAGKTDPPPVASADAPPVDQGSPGDPSAIS
                     HPDATAADEVTSLNRLVEALLLRFAHDAGGAIRGVRRGERLVEVRIHPDAVAEACQGR
                     AAMHALGQRWSVLRWVRRVRPSFVLTRRALNALDPALLQRLGNLCGSEDPRVPTGF"
     gene            522..2798
                     /locus_tag="VEW91_12340"
     CDS             522..2798
                     /locus_tag="VEW91_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYM92410.1"
                     /translation="MDAARLLEAIRADNNYRDQVVHAERFPARPARYGTLREPLSEPM
                     AEALARQGITRLYIHQAEALEAARAGESVIITTGTASGKSLCYMVPVLETLLSEPSGR
                     ALFVYPTKALAQDQLDALGEFGFDLRVGSYDGDTPQAERRRLRHEAQVLLTNPDMLHV
                     GILPQHFRWTSFLKHLRYVVLDDMHVYRGVFGSHVANILRRLIRVCRLHRADPQLVCT
                     SATVANPAEFGRRLTGRPLRVIEDDGAPRGPRTFVLWNPPVIDRARMARRSPYVEASR
                     LMALLLRRGVRTIAFTKARKITELVHRYTVDALHDTPQLAAQVSPYRAGYLPEERREI
                     EQRLFRGELRGVVATSALELGIDVGGLDAAILVGYPGTMASVWQRAGRAGRGREQSLA
                     VLIALEDALDQYLMRQPAYFFGRPIEHATLDPDNPYVLAPHLRCAASEVALWSGDDAV
                     FGPRLREVAQALEGMGDLVRRRDRWYPRSRQYPASSVEIRSVGGETYRIVEARARRLI
                     GTVEASRAFEQVHEGAVYLHQGEAYRVLRLDLSTRTATVEPDDSGSYTEARTLTDLSI
                     VHPRAERPCGGTRACFGEVLVTHQVVEYRRKQLLTDTVLSVAPLELPEEELPTTALWI
                     VIPQDIAAWVESQGLDLAGGIHAVEHAAIGLLPLFAMCDRWDIGGVSYPRYADTGQAT
                     IFIYDGHRGGVGVAEKGYEVVDELLRRTLDAIRTCPCEAGCPSCIQSPKCGNLNTPLD
                     KQAAVVLLQRLLDGAAAA"
     gene            complement(2865..3473)
                     /locus_tag="VEW91_12345"
     CDS             complement(2865..3473)
                     /locus_tag="VEW91_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92411.1"
                     /translation="MTPQPDMPSIIASPAVQRALRPRNDLREPGWTFWELRTLNMHRV
                     VGLLNGPRQFADARDLEAEIRGVVSRNFKRSWWRGMAYGVVAEVATISLSPDDLKMLV
                     DVRENEKGTLQWVILVASEARVTLGVHTWIEGYLSPVYRATLQALAETGYQVASAQRE
                     KDGLMKFLTAGADVNVALRSLGTKRVAFPEFRDPYIKENETR"
     gene            3749..4978
                     /locus_tag="VEW91_12350"
     CDS             3749..4978
                     /locus_tag="VEW91_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92412.1"
                     /translation="MGDQEKASIMQSWTTVLTLFCIASVIETIGVGQIVAFLPLDLKA
                     MGLPQTAIPRWVGLLNALMFVLGLPLVPLWGVLADKYSRKLVIARSAVVEALVFGLVA
                     LSRHPWELAGSLLLVGFQLGNTGVMLAAIRDVTPRGRLGIAIAIFGATSPIGFAAGPA
                     LGGLLLDHLHTSISTVYAVSSALSVASAAMLMVGLREIRPTVVPPGSILALAFGAMRG
                     VFTDAATRRLFALFGFALLARQMSGSFLPLAVGRVTGGGAGLASAIALVVGTAALVGG
                     IISPGAGAIGDRVGFRPVLIASLLGAGVALAVIPAAPSVSWLAATSAVVAACSASASA
                     MVFGLLVVEVPPERRSATLNLVYLPLYLAGIVGPALGALVVTAGLQAVFYAAALVVWA
                     GAALELMRPRCPAPVPE"
     gene            complement(5073..>5425)
                     /locus_tag="VEW91_12355"
     CDS             complement(5073..>5425)
                     /locus_tag="VEW91_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYM92413.1"
                     /translation="LRLVAQYADACNLHSRLTPDQLAHKLAVLRDHCERLGRPYETIE
                     KTLYGRLHVTRDYRSGTMSVPQAIAYCRTLADLGFDTLFLVDDTFNIYDPGVLDLWAS
                     ELLPAIHMIPVAGR"
BASE COUNT          799 a         1796 c         1905 g          925 t
ORIGIN      
        1 cctgcgcgcg aaaggcgaca ttcggatgcc aaacgtcgtg gaatccgccg ggaagacaga
       61 ccctccacct gttgcctccg cagacgcccc gcccgttgat caggggtccc ctggggaccc
      121 ttctgcaatt tctcacccag atgccacggc cgcggacgag gtcacgagcc tgaaccggct
      181 tgtcgaggcg ctgctgctac gcttcgcaca cgatgcgggc ggcgccatca ggggagtgcg
      241 caggggggaa cggctcgttg aggtgcgcat ccatccggat gccgtcgcgg aggcatgtca
      301 ggggagggca gccatgcacg cgctgggcca gcggtggtcg gtgctccgct gggtgcgccg
      361 tgtacgaccc tcgttcgttc ttaccaggcg ggcactgaac gcgctcgatc ctgcgctcct
      421 ccagcggctc ggcaacctct gcggctccga agacccgcgg gtcccaacgg gattctgaat
      481 gcgcacaggc ctcccccgtc cctgccccga acccactggg cgtggacgcg gcccggcttc
      541 tcgaggcgat tcgagcggac aacaattacc gggaccaggt cgtgcacgcc gagcgcttcc
      601 ctgcccgccc ggcgcgctac ggaaccctgc gcgagcccct gagcgagccg atggcggagg
      661 cgcttgcgcg acaggggatc acccgcctgt acatccatca ggccgaggct ttggaggccg
      721 cgcgggcggg agagtccgtc atcatcacga ccgggaccgc gagcgggaag tcgctgtgct
      781 acatggtgcc ggtcctcgag accctcttgt ccgaaccgtc cgggcgggcg ctgttcgtct
      841 atccgacaaa ggccctggcc caggaccagc tcgacgcgct cggggagttc gggttcgacc
      901 tgcgggtcgg cagctacgac ggagacaccc cgcaggccga acgccggcgg ctccgccacg
      961 aggcgcaggt gctgctgacc aaccccgaca tgctgcacgt ggggatcctg ccccagcact
     1021 ttcggtggac gtcgttcctc aagcacctcc ggtacgtggt gctggacgac atgcacgtct
     1081 accgcggggt gttcggaagc cacgtcgcca acatcctgcg ccggctcatc cgggtctgcc
     1141 ggctccatcg cgccgatccg cagttggtct gcacgtccgc gaccgtggcg aacccggcgg
     1201 agttcggccg gcgcctcacg ggccgcccgc tcagggtgat cgaggacgac ggcgcgccgc
     1261 gcggcccccg caccttcgtc ctgtggaacc ccccggtgat cgaccgtgcg cggatggccc
     1321 ggcggagccc ctacgtcgaa gcgagccggc tcatggccct gctcctgagg cgcggggtcc
     1381 gcacgatcgc gttcacgaag gcccgaaaga tcacggaact ggtccaccgg tacacggtgg
     1441 acgcgctcca cgataccccc cagctcgccg cgcaggtcag cccgtaccgg gcggggtacc
     1501 tgccggagga gcggcgggag atcgagcagc ggctgttccg cggcgagttg cgtggggtcg
     1561 tggcgacgag cgcgctcgag ctggggatcg acgttggcgg cctcgatgcg gcgatcttgg
     1621 tggggtaccc cggcacgatg gccagcgtgt ggcagcgggc cgggcgggcc ggtcgggggc
     1681 gggagcagtc gcttgcggtg ctgatcgcgc tcgaggacgc gctcgaccaa tacctgatgc
     1741 gccagccggc gtacttcttc ggccggccga tcgagcacgc gacgctcgac ccggacaatc
     1801 cctacgtcct ggcgccgcac ctccggtgcg cggccagcga ggtggcgctg tggtccggcg
     1861 atgacgcggt gttcggcccg aggctgcggg aggtcgccca ggcgctcgag gggatggggg
     1921 atctcgtgcg ccgccgggat cgctggtatc cccggtcccg gcagtacccg gcgagctcgg
     1981 tggagatccg cagtgtgggg ggcgagacct accggatcgt cgaggcccgc gcccggcggc
     2041 tgatcggcac ggtcgaggcg tcgcgcgcgt tcgagcaggt gcacgagggc gcggtgtacc
     2101 tgcaccaggg ggaggcctac cgggtcctgc gcctcgactt gagcacccgc acggccacgg
     2161 tcgagcccga cgacagcggg tcctacaccg aagcccggac gctcaccgat ctcagcatcg
     2221 tccacccccg ggcagagcgc ccctgcgggg ggacgcgcgc ctgcttcggc gaggtcctgg
     2281 tgacccacca ggtcgtggag taccggcgca agcagttgct caccgacacg gtcctgtccg
     2341 tcgcgccgct ggagctcccg gaggaggagt tgcccacgac cgcgctgtgg atcgtgatcc
     2401 cgcaggacat cgccgcctgg gtcgagtccc aggggctcga cctcgccggc ggcatccacg
     2461 cggtcgagca cgccgcgatc ggcctgctgc cgctgttcgc gatgtgcgac cgctgggaca
     2521 tcgggggggt gagctatccc cggtacgcgg acaccgggca ggcgacgatc ttcatctacg
     2581 acgggcaccg gggcggggtg ggggtggcgg aaaagggcta cgaggtggtc gacgagctgc
     2641 tccggcggac gctcgacgcg atcaggacgt gcccctgcga ggcggggtgc ccatcgtgca
     2701 tccagtcccc gaagtgcggc aacctgaata cgccgctcga caagcaggcc gcggtcgtgc
     2761 tcctacagcg ccttctggac ggtgcggccg ccgcttaaat atcggctatg aacggcggtg
     2821 tctcacctgg tgggtcaggg ggtgagggta gtcacccttg gatttcaccg ggtctcgttc
     2881 tctttgatat acgggtcgcg aaactcagga aaggcgaccc gtttggtgcc gaggctgcga
     2941 agggcgacat tcacgtcggc accggccgta aggaacttca tcaggccgtc cttctcccgc
     3001 tgggcgcttg cgacctggta ccccgtctcc gccagcgcct gaagtgtggc acggtacacg
     3061 ggactcagat acccttcgat ccacgtgtgc acgccgagag taacgcgggc ctcgctggcc
     3121 acgagaatga cccactgcag ggtgcccttt tcgttctcac gaacatcgac cagcatcttc
     3181 aggtcatcgg ggctcaagga gatcgtcgcg acctccgcaa cgaccccgta cgccatcccg
     3241 cgccaccagg aacgcttgaa gttgcgcgag acgactcctc ggatctccgc ttcgagatcg
     3301 cgtgcgtccg cgaactgtcg gggcccgttc aagagtccga ccacacggtg catattgagg
     3361 gtgcgcagct cccagaacgt ccagccgggc tcgcggagat cattgcgggg tcgcagggct
     3421 cgctgcaccg ccggcgatgc aatgatggag ggcatgtcag gttggggtgt catcggctcc
     3481 tccctatcca agtttagcgg ttcagcaacc tgctggattt gtggcaaaga gttgtcgagc
     3541 cagcatggtg ctctccgccc tgctgtagga tacccaggcc tgccgtgggg tggacttcga
     3601 tatcgagccg cgcacgggcc ctaagcaata acaacgagga agcccaagat gctcacctac
     3661 gttgaccttt caggaaaatg aacgttggaa gatattcgcc tgcttccccg cgcaggcggg
     3721 ccaacctctg ggcggaaggg atgtgcgagt gggggatcaa gaaaaggcaa gcatcatgca
     3781 gtcctggacc accgtcctta cgctgttctg catcgcgtcg gtcatcgaga cgatcggcgt
     3841 cggccagatc gtcgcattcc tgccgttgga cctcaaggcg atggggctac cccagacggc
     3901 gatcccccgg tgggtgggcc tcctcaatgc gctcatgttt gtccttggac ttcccctggt
     3961 ccccctatgg ggggtgttgg cggacaagta cagccgcaag ctcgtcatcg cccgcagcgc
     4021 ggtcgtcgaa gcgctggttt tcggtctcgt cgcgctgagc cgccatccct gggagctcgc
     4081 aggcagcctg ttgctggtgg gtttccagtt ggggaacacg ggggtgatgc tggcggcgat
     4141 ccgagacgtg acgccgcggg gccgcctcgg gatcgctatc gcgatcttcg gcgccacatc
     4201 accgatagga ttcgccgcgg gcccggcgct gggcgggctc cttttggatc acctccacac
     4261 ctccatctca accgtctacg cggtctcttc cgctctctcg gtggcgagcg ccgcgatgct
     4321 tatggtgggc ttgcgggaga tccgcccgac cgtggttccg ccgggatcga ttctcgccct
     4381 ggccttcggg gcgatgcggg gcgtatttac cgatgccgcg acgcgccgtc tgttcgctct
     4441 cttcgggttt gcgttgctgg cccggcagat gtcggggtcg tttcttccgc tcgcggtcgg
     4501 gcgcgtgacc ggtggcgggg ccgggctggc gagcgcgatt gcgctagtcg tcggcacggc
     4561 ggccctggtc ggtgggatca tttctccggg agcgggggcg atcggcgacc gcgtcggctt
     4621 tcgccccgtg ctgatcgcat cgttgctcgg cgcgggcgtg gcgctggcgg tgatccccgc
     4681 cgccccgagc gtgtcctggc tcgccgcgac gagcgccgtc gtggcggcat gcagcgcctc
     4741 ggcgagtgcg atggtgttcg gtcttctggt cgtcgaggtt ccgccggagc gccggtcggc
     4801 gacgctcaat ctcgtctacc tgccgctcta cctcgcggga atcgtggggc ctgctctggg
     4861 ggcgctcgtc gtcaccgcgg ggctccaagc ggtgttctac gcggccgcgc tcgtcgtctg
     4921 ggcaggagca gcccttgaac tcatgcgccc gcgctgcccc gccccggtgc ccgagtgacc
     4981 tgctcccacc ggccctccac ccaagatcgg ccggctgact cacgcccagg cgctcgggcg
     5041 ctcaggacgg aagtcctgtg gttgaggcca actcagcgcc cggcaacggg gatcatgtgg
     5101 atggctggga gcagttcgga cgcccagagg tcgagcacgc ctggatcgta gatgttgaag
     5161 gtgtcgtcca ccaggaagag ggtatcgaag cccagatcgg ccagggtccg gcagtaggcg
     5221 atggcctgcg gcaccgacat cgtgccgctg cggtagtcgc gggtgacatg gaggcgacca
     5281 tacagggtct tctcaatggt ctcgtagggg cggcccagcc gctcgcagtg gtcccgcagc
     5341 accgccagtt tgtgggcgag ttggtcgggg gttaagcggc tgtggaggtt gcaggcatcg
     5401 gcgtactgcg cgacgagacg tagtg
//
LOCUS       DASQGH010000621         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53579, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000621 DASQGH010000000
VERSION     DASQGH010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53579"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VEW91_12360"
     CDS             <1..470
                     /locus_tag="VEW91_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HYM92414.1"
                     /translation="VAFLYDKAAGRPNRFGTPDEFPIVATTYRLTEHEHYVTQHVPHL
                     VQLQPEAFVEIPEGLAREKGIKNGDMVRVSSKRGKIEVRALVTGRLGQLAVGGKQVWG
                     VGIPIHWGFVGISAEMHPEKSQHWLANALTPFVGDANARTPEFKAFLVNIEKL"
     gene            470..1345
                     /gene="fdhE"
                     /locus_tag="VEW91_12365"
     CDS             470..1345
                     /gene="fdhE"
                     /locus_tag="VEW91_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HYM92415.1"
                     /translation="MAPPALLVEPWTARRRRTEALRERYPFALEILDLYRALLDVQEP
                     AFAAARADRPAASGLPGYVVERVLPAVADTTCARGPVRLSALVRRRVEGGDLAELVSG
                     WLIGVEQSPVDRFLARAATTPVLEALGEAAGAACPGPRDRRHCPICGGLPQVSYSAPS
                     AEALVTSQRHLVCARCMTSWPYPRMTCAACGETEGSRLTVYAEEGAAEAQLSGRIIRG
                     VGAVEPEGPLGDRPRFPHIRIEACTTCSRYLLGVDLTRDPRAVPVVDDLAAVPLDLYA
                     VDRGFAKIMPNFMGV"
     gene            1349..>2002
                     /locus_tag="VEW91_12370"
     CDS             1349..>2002
                     /locus_tag="VEW91_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYM92416.1"
                     /translation="MPEPVGFFTDTSICIGCKACEVACKEWNQLPGHAPAFLDSFDNT
                     GKLDEQNWRHVQFHDRVPDGPATAGNGRPWLMMSDVCKHCTQASCMEVCPTGAIIRTE
                     FDTVFIQQDVCNGCRDCIAACPFGVIGFSEATGTVHKCTFCYDRLQAGKMPACAQACP
                     TQSIMFGPLSELRKKAGARLEAIHAQGYTDAQLYGHDDKIYGGLSAFFLLMDKPEVYG
                     "
BASE COUNT          293 a          698 c          701 g          310 t
ORIGIN      
        1 cggtcgcgtt cttgtacgat aaggccgccg gccggcccaa tcgcttcggc acgcccgacg
       61 agttccccat cgtcgcgacc acgtaccggc tcaccgagca cgagcactac gtcacgcagc
      121 atgtccctca cctggtccag ctccagccgg aggccttcgt cgagatcccc gaagggctcg
      181 cgcgcgagaa ggggatcaag aacggcgaca tggtccgcgt ttcgtccaaa cgcgggaaga
      241 tcgaggtgcg cgcgctggtg accgggcggt tgggccagct cgccgtgggc ggcaagcagg
      301 tgtggggggt gggcatcccg atccactggg ggttcgtcgg catctcggcg gagatgcatc
      361 cggagaagtc ccagcactgg ctggccaacg cgctcacgcc cttcgtgggc gacgcgaacg
      421 cgcggacgcc cgagttcaag gcgtttctcg tcaacatcga gaagctctga tggcgccgcc
      481 cgccctgctc gtcgagccct ggaccgcccg ccggcggcgc accgaggcgc tgcgcgaacg
      541 ctaccccttt gccctcgaga tcctggacct gtaccgggcc ctcctggatg ttcaggagcc
      601 ggcgtttgcg gccgcgcggg cggaccggcc ggccgcctcc ggcctccccg gctacgtcgt
      661 cgagcgcgtc ctcccggcgg tcgcagacac gacctgcgcc cgcgggccgg tacggctctc
      721 ggcgctcgtg cgccgccggg tcgagggtgg ggacctcgca gagctcgtct cgggctggct
      781 catcggggtg gagcagtccc cggtggaccg cttcctcgcc cgcgccgcga ctaccccggt
      841 gctcgaagcc ctcggcgagg ccgcgggcgc cgcctgcccc gggccgcgag accggcgcca
      901 ctgcccgatc tgtggcgggc tgccgcaggt cagctactcc gctccctcgg ccgaggccct
      961 ggtgacttct cagcggcacc tggtgtgcgc gcggtgcatg accagctggc cctatccgcg
     1021 gatgacctgc gcagcgtgcg gcgagaccga gggttcccgg ctcacggtct acgccgagga
     1081 gggcgcggcc gaagcccaac tctccggtcg catcatccgc ggggtcgggg cggtcgagcc
     1141 ggaggggccg ctgggagacc ggccgcggtt tccccacatc cggatcgaag cgtgcacgac
     1201 ctgctcccgg tatctgctcg gcgtggatct gacccgcgac ccccgggccg tgcccgtggt
     1261 ggacgatctg gccgcggtgc cgctcgacct gtacgcggtc gaccggggct ttgcgaagat
     1321 catgcccaac tttatggggg tatgagcgat gcctgaacct gtcggcttct tcaccgacac
     1381 gagcatctgc atcgggtgca aggcgtgcga ggtcgcctgc aaggagtgga accagttgcc
     1441 ggggcacgcg cccgcgttcc tcgacagctt cgacaacacg gggaagctgg acgagcagaa
     1501 ctggcgccac gtgcagttcc acgaccgggt ccccgacggc ccggccaccg cgggcaacgg
     1561 gcgcccgtgg ctcatgatgt cggacgtgtg caagcactgc acgcaggcca gctgcatgga
     1621 ggtctgtccg accggggcca ttatccggac ggagttcgat acggtcttca tccagcagga
     1681 tgtgtgcaac ggctgccgcg actgcatcgc ggcctgcccc ttcggcgtga tcgggttcag
     1741 cgaggcgacg gggaccgtgc acaagtgcac cttctgctac gacaggctcc aggcgggcaa
     1801 gatgccggcc tgcgcccagg cctgccccac ccagtcgatc atgttcgggc cgctctccga
     1861 gctcaggaag aaggccgggg ccaggctgga ggcgatccac gcccagggct acacggatgc
     1921 ccagctttac gggcatgacg acaagatcta tggcgggctg agcgcgttct tcctgctgat
     1981 ggacaagccg gaggtctacg gg
//
LOCUS       DASQGH010000622         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22432, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000622 DASQGH010000000
VERSION     DASQGH010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22432"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..627
                     /locus_tag="VEW91_12375"
     CDS             265..627
                     /locus_tag="VEW91_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92417.1"
                     /translation="MRGKLFLIHWNPPLAEQMARDLRAAEWRVDFESEDSTRAVRRIR
                     EQSPNAIVISLARSPSHGVETAQALRAMRVAGVPIVFVDGKADTIKEAKARVPMAIFA
                     TSGDLSDILATYAKFGWG"
     gene            complement(631..924)
                     /locus_tag="VEW91_12380"
     CDS             complement(631..924)
                     /locus_tag="VEW91_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92418.1"
                     /translation="MDDLEARKVLKVFGMQVTAFMGRRRELTEQAATAIVDHDRQTLT
                     ALLATLMSETSELHRRWLEVTNLVLQEEREAYSEMARLLEEAGQTEKTTTSEA"
     gene            1042..1629
                     /locus_tag="VEW91_12385"
     CDS             1042..1629
                     /locus_tag="VEW91_12385"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HYM92419.1"
                     /translation="MTLGGVILAGGESRRMGRPKALLPLGDTTLIGAVIARLREACPQ
                     ILIVTNTPELYRPLGVRLVPDALPGRRSLVGIYTGILHTGGPTFVCACDMPFLNPALI
                     RYLGALAEAADVVIPRHGGDYEPLHAVYTPACLEPIRRRARSGERNTGFLEEVRARVV
                     EVEELRRLDPDLRSFINVNTPEDYARLQGMPPGRG"
     gene            complement(1630..>4282)
                     /locus_tag="VEW91_12390"
     CDS             complement(1630..>4282)
                     /locus_tag="VEW91_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HYM92420.1"
                     /translation="PVYWVPIVSSAVVGFAAGLAVFLILFLNVRPLLGLRPMPKVIEL
                     RSSGGRAYRQVVSRLRPVAVTAVGVGVVSLLAGRGAATSWLTFQGWLHRLPFGVRDPV
                     FNLDIGFYVFTLPAYTAVYDWLFSWLFIALLIAATGYYLDLAPILMRGVWAIPRAARV
                     HLAVLTGLLLLLRAAGFWLDAYGLLFSPRGALYGAGYTDLHATLPALRLLTALAGVSG
                     VLMLASVWLRTIRLAVGSLLLMIVVWIAGTGVYPSIVQQFEVAPNELNREMPYIRNAI
                     AAALHAFDLDQAQVQPFPATESLTPTTVRANQEVLDSVRLWDYRPLLQTYAQLQGLRL
                     YYTFTSVGIDRYQIGGHQQQVMLSAREMDISRLTSEARTWVNDHLVFTHGYGLVMNPV
                     NRISAEGLPEFYIKDIPPQSPIGLRVDRPELYYGLLASQYVIVKTRAKELDYPQGEQN
                     VYTNYGGRGGVALTAPLAKLAFMARFGASQVVLSNDVTAESRVMFHREVRERVAHLAP
                     MLSFDHDPYLVLDGGRLVWIVDAYTTTSGYPYSRPSAGLNYIRNSVKAVVDAYDGTTA
                     FYVVDPKDPLIQVYGRIFPGLFRPLAALPPGLVAHLRYPQDLFTIQAQVYSTFHMTDP
                     RVFYNREDLWTFPNELFTGSAQPLEPYYVNLKLDPARGEEFALILPFTPVGKDNMVAW
                     MAGRSDAPNYGRLLVYRFPKDRTVFGPMQIEARINQDPTISSQLALWNQQGSRVIRGN
                     LLVVPIADALLYIEPLYLQAAGSALPELKRVLVAYGPRIAMEPTLEAALARIFVGLPS
                     GPAAGSPGQPASPGPGASAAPPPSTAPGAARGRMAALAAEASAHYDRAQAALRAGDFA
                     AYGREIDALGRVLAELKRAAESPQDRP"
BASE COUNT          720 a         1434 c         1502 g          626 t
ORIGIN      
        1 gcgtgcggtg cggagcacac cacagatcgc tcgctcgccc gctccttctg gcaccgatgg
       61 caagagtcct ttcagcgctt tggaactccc cacgagattc gggaggaccc ctcgggcgat
      121 tcccgcgggg tttttcgcaa cagattcgag gaatgatgga accgattgca aaggagttcg
      181 aaaggatccc ggtcaagact ggagtgctgg tcttttggag tccgtattcg gaggcacccc
      241 gttcacgggt gaaaggagga gctcatgcga ggcaaactct tcctgattca ctggaaccct
      301 cccctggcag agcagatggc gcgagacctg cgcgccgcgg aatggcgcgt cgacttcgag
      361 tcggaagata gcacgcgggc cgtgcggcgg atcagggaac aatcccccaa cgcgattgtg
      421 atctcgctgg cccgctcgcc gtctcacggc gtggagaccg cccaggccct ccgcgccatg
      481 cgggtcgcgg gcgtcccgat cgtgtttgtg gacggcaaag cggacacgat caaggaggcc
      541 aaggcccggg tgccgatggc catctttgcg acgtcgggcg acttatcaga tatcctggcg
      601 acgtacgcga agttcggctg ggggtaggcg tcaggcttcg gaggtggttg tcttttccgt
      661 ctggccggcc tcctcgagca ggcgcgccat ctcgctgtag gcttcgcgct cttcttggag
      721 gacgaggttg gtgacctcca gccaccggcg gtgcagctcc gaggtttcgg acatcagggt
      781 ggcgaggagc gcggtcagcg tctgccggtc gtgatccacg atggcggtgg ccgcctgctc
      841 ggtcaactcg cggcggcgcc ccatgaacgc cgtcacctgc atgccgaaca ccttgagcac
      901 ctttcgcgcc tcaaggtcgt ccatcgccga ccctccttgg cccgaaaccc cgtgtgcgag
      961 gattccccct gtggcggcca aaaccctcgc cgggggccgg cctccggagg gagcggcaaa
     1021 tctggagcag aaggggccgg catgaccctc ggcggcgtga tcctggccgg cggcgagagc
     1081 cggcgcatgg gccggcccaa agcgctcctg cccctcgggg acaccacgct aatcggcgca
     1141 gtcatcgcgc ggctccgcga ggcgtgcccc cagatcctga tcgtcaccaa cacgccggag
     1201 ctctaccggc cgctgggggt tcgcctggtg ccggacgcgc ttcccgggcg ccggtcgctc
     1261 gtgggcatct acaccgggat cctccacacc ggcgggccga cgttcgtctg cgcctgcgac
     1321 atgccgttcc tcaacccagc gctcatccgc tacctcggcg cgctcgccga ggcggcggat
     1381 gtggtgatcc cgcggcacgg cggagactac gaacccctgc acgccgtcta cacgccggcc
     1441 tgcctggaac cgatccggcg gcgcgcacgc agcggggagc gcaacacggg gttcctcgag
     1501 gaggtccggg cgcgggtcgt cgaggtggag gagctccggc ggctcgaccc ggatctgcgc
     1561 tcgttcataa acgtcaacac cccggaggac tacgcgcggc ttcaggggat gccgcccgga
     1621 cgagggtgac taggggcggt cctggggaga ttcggcggcc cgcttgagct ccgccagcac
     1681 ccggccgagc gcgtcgatct ctcggccgta cgcggcgaag tccccggccc ggagcgcggc
     1741 ctgcgcccga tcgtagtgcg cgctggcctc ggcggcaagc gcagccatcc gcccgcgagc
     1801 ggccccgggg gcagtagacg gcggcggcgc cgcggacgcc cccgggcccg gggaagcggg
     1861 ctggccaggg gatccagccg cgggtccgga gggcagcccg acgaagatgc gcgccagcgc
     1921 ggcctcgagc gtcggctcca tcgcgatccg gggcccgtag gcgacgagaa cccgcttgag
     1981 ctccgggagc gcgcttccgg cagcctgcag gtacagcggc tcgatgtaca agagcgcgtc
     2041 ggcgatcggc acgacgagca ggttgccgcg gatcacccgg gagccctgct ggttccacag
     2101 cgcgagctgg ctgctgatcg tcggatcctg gttgatccgc gcctcgatct gcatcggacc
     2161 gaacaccgtg cggtccttcg gaaaccggta cacgaggagc cggccgtagt tcggcgcgtc
     2221 gctccggccg gccatccagg ccaccatgtt gtccttgccg acgggggtga acgggaggat
     2281 cagcgcaaac tcctcgcccc gcgccggatc cagtttcagg ttcacgtagt acggctccag
     2341 cggttgcgcg gatccggtaa acagctcgtt gggaaacgtc cagaggtcct cgcgattgta
     2401 gaacacgcgc gggtccgtca tatggaacgt cgagtagacc tgcgcctgga tggtgaacag
     2461 gtcctgcggg taccggaggt gcgcgacgag ccccggcggg agcgccgcga gcggccggaa
     2521 cagaccgggg aagatcctcc cgtacacctg gatcagtgga tccttcggat ccacgacgta
     2581 gaacgcggtg gtgccgtcgt aggcgtcgac gaccgccttc acggagttgc ggatgtaatt
     2641 gaggccggcg gacggccgcg agtacgggta ccccgaggtc gtcgtgtagg cgtccacgat
     2701 ccagaccagc cgaccgccgt ccagcaccag gtacggatcg tggtcgaagc tcagcatcgg
     2761 cgcgaggtgg gccacccgct cgcgcacctc ccggtggaac atcacccggc tctcggcggt
     2821 gacgtcgttg ctcagcacca cctgggaggc gccgaaccgg gccatgaacg ccagcttggc
     2881 cagcggggcg gtgagcgcca cgcccccgcg cccgccgtag ttcgtgtaga cgttctgctc
     2941 gccctggggg taatcgagtt ccttggcccg cgtcttgacg atcacgtact ggctcgcgag
     3001 gagcccatag tacagctccg gccggtcgac gcggaggccg atcgggctct gcgggggaat
     3061 gtccttgatg tagaactcgg gcagcccctc cgccgagatc cggttcaccg ggttcatcac
     3121 gagcccgtac ccgtgggtga acaccaggtg gtcattgacc cacgtgcgcg cctcggaggt
     3181 gagccgcgag atgtccatct cccgggccga cagcatcacc tgctgctggt gccccccgat
     3241 ctggtaccgg tcgatcccca cgctcgtgaa cgtgtagtac agccggaggc cctggagctg
     3301 ggcgtaggtc tgcaggagcg gccggtagtc ccacaaccgg acgctgtcga gcacctcctg
     3361 gttcgcccgc acggtcgtgg gggtcaggct ctccgttgcg gggaacggct ggacctgggc
     3421 ctggtccagg tcgaacgcgt ggagggccgc ggcgatggca ttccggatgt acggcatctc
     3481 acggttcagc tcgttcgggg caacctcgaa ctgctggacg atcgaggggt acaccccggt
     3541 ccccgcgatc cagacgacga tcatcaggag caacgagccg acagccaggc gaatggtccg
     3601 cagccagacg gaggccagca tgaggacgcc ggagacaccg gcgagcgccg tcagcaggcg
     3661 gagcgcaggc agggtggcgt ggaggtcggt gtatcccgct ccgtagagcg cgccgcgggg
     3721 ggagaacaag agcccgtagg cgtcgagcca gaacccggcc gcccgcagca ggagcagcag
     3781 cccggtgagc acggccaggt gcacccgggc cgcccgcggg atcgcccaca ccccccgcat
     3841 caggatcggc gccagatcga gatagtaccc ggtcgcggcg atgaggaggg cgatgaacaa
     3901 ccaggagaac agccagtcgt acaccgcagt atacgccggc agggtgaaca cgtagaaccc
     3961 gatgtcgagg ttgaacaccg gatcgcggac gccgaacgga aggcggtgca gccacccctg
     4021 gaacgtcaac cacgacgtgg cggcgccccg gccggccagc aggctcacca cgccgacccc
     4081 gacggcggtc accgccacgg gacgcaggcg ggacaccacc tgtcggtacg cgcggccccc
     4141 cgaggaacgg agctcgatca ccttgggcat cggcctgagg cccaggaggg gccggacgtt
     4201 caggaacagg atgaggaaga ccgcaaggcc cgccgcgaat cccacgacgg cgctcgagac
     4261 gatgggcacc caatagaccg ga
//
LOCUS       DASQGH010000623         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_36735, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000623 DASQGH010000000
VERSION     DASQGH010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36735"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..832)
                     /locus_tag="VEW91_12395"
     CDS             complement(104..832)
                     /locus_tag="VEW91_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92421.1"
                     /translation="MKEILSLGAVNTYRGPAHILRDVSLVVGEGETVCLIGRNGAGKT
                     TAMESVMGLLPLRSGTITLGGQEITRLGPHMRARRGIGYAPDDCGIFPELTVAENLGI
                     GSWLAETSHRAGGGGAQAARGEAIYALFPELPRLLQRRGLHLSGGERKMVAIARCMAL
                     GPSVLLLDEAFEGLAPIVVNRFAEAVQKIRAMGISMLIAESNVTTAAKVADRFYVIDR
                     GEIIFQGAPAEVFANKEIMQVIRG"
     gene            complement(868..1611)
                     /locus_tag="VEW91_12400"
     CDS             complement(868..1611)
                     /locus_tag="VEW91_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92422.1"
                     /translation="MPLLATEGLWKSFGDMHAVHDVGFSVDEKQLVALVGSNGAGKTT
                     LVNLITGLLRPDRGSIHFQGQDVTALPVYDRIKAGITRSFQIVNLFDALTTLDNVRLA
                     IFSREGKVGKAASLAERDRDAREQALGVIDEFGLTPKWDVPAGQLAQGERKLLDVAVA
                     YALGPRLILLDEPTSGVGTREKGQIMDRIEAVVRSRGLSAVIVEHDMDVVFKYSDRMV
                     VMHEGQVLAEGVPDQIVRDERIADMLGRR"
     gene            complement(1612..>2419)
                     /locus_tag="VEW91_12405"
     CDS             complement(1612..>2419)
                     /locus_tag="VEW91_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYM92423.1"
                     /translation="LLSFGHSAKFGAAAYDAGFVVKYLNTTSMEVVLAGGLVSGFVIA
                     ALFGFVCVRYTRIFFSILKLALSQVLWSLALKFFWVTGGTDGIRVPTPSLLGGLVPSS
                     GDKVAFLAHGYYYYDLVGFCLSVAAMALIAHSPFGKALQAIRDNEVKAEFIGIRVRRF
                     RWMAFVISGVFTGVAGALWAPLNGLTTPEALYWPFSGEIVFMTVLGGFRTFTGPAVGA
                     VVFNYLKTYAVGRTEYWQLLLGVILITLVLVMPAGILGTLARFTRTSRRD"
BASE COUNT          431 a          881 c          717 g          390 t
ORIGIN      
        1 ggatcgcggc ccacgtgctc tgcattaccc cacgcgccac gcgccccagg ccccgcatca
       61 accgatcacc ccccggtcgt gcgcggcgcg gcggtcatcc gggtcatccc cgaatcacct
      121 gcatgatctc tttattggca aacacctctg ccggggctcc ctggaagatg atctccccgc
      181 gatcgatcac atagaagcga tccgccacct tcgcggccgt cgtgacgttc gattcagcga
      241 tcagcatgga tattcccatg gccctgatct tctgcaccgc ctcggcgaag cggttgacga
      301 cgatgggcgc gagcccttcg aacgcctcgt cgagcagcag gaccgatggt cccaaagcca
      361 tgcatctggc gatcgccacc atctttcgtt ccccaccgct tagatgcagc cctcttcgct
      421 ggaggagccg tggcagttcg gggaagagcg catagatggc ctctccccgg gccgcttgcg
      481 ccccgccgcc gcccgcccgg tgggacgtct cggccagcca ggagccgatc ccaaggttct
      541 cggccaccgt caattcgggg aagatcccgc aatcgtcggg cgcatacccg atcccccgcc
      601 gcgctctcat gtgggggccg agccgggtga tctcctgccc gccgagcgtg atcgtcccgg
      661 accgcagggg cagaagtccc atgacgctct ccatcgccgt ggttttcccc gccccattcc
      721 tgccgatcag gcaaaccgtc tcgccctccc cgaccaccag ggagacatcg cgcaggatgt
      781 gggccggccc gcggtacgtg ttgaccgcgc ccaggctgag gatctccttc acggccgccc
      841 cgccatcctc acgacgcttg cataccccta ccggcgcccc agcatgtcgg cgatgcgctc
      901 gtcgcggacg atctggtcgg ggaccccttc ggcgagcacc tggccctcgt gcatgacgac
      961 catccggtcc gaatacttga agacgacgtc catgtcgtgc tcgacgatca ccgcgctgag
     1021 tcccctcgac ctcaccacgg cctcgatccg gtccatgatc tggccctttt cccgcgtgcc
     1081 gaccccgctc gtcggctcat cgagcaggat caacctcggc ccgagcgcgt acgcgaccgc
     1141 cacgtcgagc agtttgcgct cgccctgcgc cagctggccg gccgggacgt cccacttcgg
     1201 ggtgaggccg aattcatcga tcacgccgag cgcctgctcc cgtgcatccc gatcgcgctc
     1261 ggccagcgac gcggccttcc ccaccttccc ctcgcgagag aagatcgcca gccggacgtt
     1321 gtccagcgtg gtgagcgcgt cgaacaggtt gacgatctga aagcttcgcg tgatcccggc
     1381 cttgatccgg tcgtagacgg ggagcgcggt gacatcctgt ccttggaaat ggatgcttcc
     1441 gcgatcgggc cgaaggaggc cggtgatcag gttgaccagg gtcgtcttcc cggccccgtt
     1501 cgaccccacg agggccacca actgcttctc gtcgacggag aagcccacgt cgtggaccgc
     1561 gtgcatgtct ccgaaggact tccacaggcc ttccgtcgcc agcagcggca ctcagtccct
     1621 cctcgacgtt cgcgtgaagc gcgcgagggt gccgaggatc cccgcgggca tgaccaggac
     1681 cagcgtgatc aggatcacgc cgagcagcaa ctgccagtac tcggttcgcc cgaccgcata
     1741 cgtcttgaga tagttgaaga ccaccgcccc gacggcggga ccggtgaagg tcctgaatcc
     1801 ccccagcacg gtcatgaaga cgatctcccc cgagaacggc cagtagagcg cctccggggt
     1861 cgtcaggccg ttcagcggag cccagagcgc ccccgccacc cccgtgaaga cccccgaaat
     1921 cacgaacgcc atccagcgga accttctcac gcgaatcccg atgaactccg ctttgacttc
     1981 gttgtcgcgg atcgcctgga gggccttccc gaacggcgag tgcgcgatca gcgccatggc
     2041 ggcaacgctc aggcagaagc ccaccaggtc atagtagtag tacccgtggg cgaggaacgc
     2101 caccttgtct cccgaggaag ggaccagccc gcccagcagg gacggcgtgg gcacccgtat
     2161 cccgtccgtc cctccggtca cccagaagaa cttcaatgcg aggctccaca ggacttggga
     2221 aagcgccagc ttgaggatgg agaagaagat ccgggtgtac cgcacgcaga cgaagccgaa
     2281 aagggccgcg atcacgaacc cggagacgag gcccccggcc aggaccacct ccatcgaggt
     2341 cgtattcaag tatttcacca caaagccggc gtcataggcg gccgccccga acttcgccga
     2401 gtggccgaag gacagcagg
//
LOCUS       DASQGH010000624         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_2726, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000624 DASQGH010000000
VERSION     DASQGH010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VEW91_12410"
     CDS             complement(<1..538)
                     /locus_tag="VEW91_12410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYM92424.1"
                     /translation="MALAAVLRFAWLGRHSVFFDEAYVAWLSAHRWSDLLLLRLAEYH
                     PPLHYVLMKAWIALAGPGEAALRTPSAVFGSLSVLLTYFLVRRVASEHVSLMSALFVA
                     VSPFEIMAGQYGKMYTLLEVLAVGSTLVLALATERGAPRWTLYVALAAATLYTHYLGV
                     FVLAAHGAWVLWRRRPRLT"
     gene            complement(926..2692)
                     /locus_tag="VEW91_12415"
     CDS             complement(926..2692)
                     /locus_tag="VEW91_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HYM92425.1"
                     /translation="MRQCATALATALVLAMIAPAFAQPFADTPTNHWAYDAIAELAAK
                     GLIEGYPDGTFKGDRAMTRYEMAMVVARLLARIESIQIPTPAPAMRPEVGRTDIDTIL
                     RLVNEFRAELAAKNVRLTAVEEELNALKARLDNVKITGRFLWRYDGRPRSGGASLNGN
                     PNVGATDASKFATIFLSHEGLKLSVDGSVAPDVHAILGLELASNAHRTPLTFNSSNLG
                     TVNLTNPTGGTTTSPQYVLANIVELFFDWKNIWGTPIELWIGRFGGGVQGFASYPVQF
                     GPMGLLLKTNTNNWGASTGNSGANLADGLRLAGHWAELADLQIQGVIIRVTGPTGSTT
                     YNLGEDAYGIDANIQIMPGVRGGGYYVANTTNAGSTVTVSPLGALYHVYGNLDSPSQD
                     PVTPLCPAVATGALSSTGIAVVQGGITCAAAGSGWGGYVQWDVMPGIHFDGEYAQWTD
                     SVHAISDSAFMTNVAWDLGTLLGVGHNFSVQTGYAYAGTNFYAPYGTDFDDNINGSIF
                     PGDFQGFVGGVSYDLFSNLTLLGQWINGDNVFNGQTVTAWQAGFIYKFAPGTQIWIRY
                     ETYSVAGVAFYNLYRAELNYTF"
BASE COUNT          496 a          963 c          969 g          525 t
ORIGIN      
        1 atgtgaggcg ggggcggcgg cgccacagca cccatgcccc gtgcgccgcg aggacgaaga
       61 cgccaaggta gtgcgtgtac agcgtcgctg ccgccagggc gacgtagagg gtccaccgcg
      121 gcgcgccgcg ctccgtggcc aaggcgagca ccagcgtgga tccgacggcc agaacctcca
      181 gcagcgtgta catcttgccg tactgccccg ccatgatctc gaacggtgag acggccacga
      241 acaacgcgct catgagactc acgtgctccg aagcgaccct gcggacgagg aagtacgtca
      301 gcaagacaga caggctgccg aacacggccg acggggttcg cagcgcggcc tcccccggtc
      361 cggccagggc aatccatgcc ttcatcagga cgtagtggag cggagggtgg tactcagcga
      421 ggcgcagcag cagcagatcg ctccaccggt gggccgagag ccatgcgacg tatgcctcgt
      481 cgaagaaaac gctgtgccgg ccaagccatg cgaaccggag gacggccgca agtgccaaca
      541 gaccagcggg gacggctctg gagctcaggg gcacggcgcc tccacggcac atcgcttctg
      601 cctggcggca agattgcagc cgcggccatc acgtcgaaat ttccggtttg gctcctgagt
      661 tccttccaac cgaaatgtcc gttgtaggcg ctctgttcct gctggccgta gaccgggatc
      721 tcggtgctgt ccatatcgag caccgcccgc ggcgcggact ccttggcttc cacccgcgtc
      781 ggttgatcgc ggccaagcct gcgaggttct cgtcttgcgt caacagctcc gcctcgaagg
      841 agtgcacacg catgcctact ttgtgcaggt ggccccccgg attctccggg aggccacccc
      901 agaccgaccg cgtctgtcgg tcccgctaga acgtgtagtt caactccgcg cggtagagat
      961 tgtagaacgc gacgcctgcg accgagtacg tctcataacg gatccagatc tgggtgccgg
     1021 gcgcgaactt gtagataaac ccggcctgcc acgctgtgac ggtctggccg ttaaagacgt
     1081 tgtcgccatt gatccattgc ccgagcaggg tcaggttgga gaacagatca taggagacgc
     1141 ccccgacgaa gccctggaag tcaccgggga agatcgagcc gttgatgttg tcatcaaagt
     1201 cggtgccgta cggggcgtag aagttcgtgc ccgcgtacgc gtagccggtc tggaccgaga
     1261 agttgtgccc gacgcccagc agggtcccga gatcccatgc cacgttggtc ataaacgcgc
     1321 tatcgctgat cgcgtgcacg gagtcggtcc actgcgcgta ctcgccgtcg aagtggatcc
     1381 ctggcatgac atcccactgg acgtagccgc cccaacccga gcccgccgcg gcgcaggtga
     1441 tgccgccctg gaccaccgcg atgccggttg agctgagcgc gccggtggcg accgccgggc
     1501 agagcggcgt aaccgggtcc tgggaagggg agtcaaggtt cccatacacg tgatagagcg
     1561 caccgagcgg gctcacggtc actgtgcttc ccgcgttggt ggtgttggcc acgtagtagc
     1621 cgccaccccg gacgcccggc atgatctgga tgttcgcgtc gatgccgtag gcgtcctcgc
     1681 ccaggttgta ggtcgtggag cccgttgggc cggtcacccg aatgatcacg ccctggatct
     1741 ggaggtccgc gagctcggcc cagtgcccgg cgaggcgcag gccgtccgcg aggtttgcgc
     1801 cgctgttgcc ggtcgaggct ccccagttgt tcgtgttggt cttcagcagg agccccatcg
     1861 ggccaaactg cacggggtac gaggcgaagc cttgcacgcc tccgccgaag cggccgatcc
     1921 agagctcgat cggcgtcccc cagatgttct tccagtcgaa gaacagctcg acgatgttcg
     1981 cgagcacgta ctgcgggctg gtcgtcgtgc cccccgtggg gttggtcagg ttgaccgtcc
     2041 cgaggttgct gctgttgaag gtgaggggcg tgcggtgggc gttagaggcg agctcgaggc
     2101 cgagaatcgc gtgcacatcc ggcgccacgc tgccgtccac cgagagcttg agcccttcgt
     2161 gtgaaaggaa gatcgtggca aacttggatg cgtcggtagc acccacgttg gggttgccgt
     2221 tcagggaggc gccacccgac ctaggacggc cgtcgtagcg ccacaggaac cggcccgtga
     2281 tcttgacgtt gtcgagccgg gccttgagcg cattcagctc ttcctcgacc gcggtcaacc
     2341 ggacgttctt cgcggccagc tcggcccgga actcgttcac cagccggagg atggtgtcga
     2401 tatccgtcct gccgacctcc ggcctcatgg ccggcgccgg cgtcgggatc tggatgctct
     2461 cgatccgcgc caggagccgc gcgaccacca tagccatttc gtaccgcgtc atggctcgat
     2521 cgcccttgaa ggtcccatcg gggtagccct cgatgagccc cttcgccgcc agctcagcga
     2581 tcgcgtcgta cgcccagtgg ttggtgggcg tgtcggcgaa cggctgcgca aaggcggggg
     2641 cgatcatggc gagcacgagc gcggtcgcga gtgcggtcgc acactgtctc atgtcgcttg
     2701 tcccccttgc gcgttgtgag atcgggggat ggcagggacg gggtgactcg agtgtccgtg
     2761 ttgtctcttg ccgcccctac gccctgacgt ctccccgcgt gccctctccc cggatcctca
     2821 ccctccccta ccttcctttc cttcagatcg aagtccacga ccgccttacg ctcagcgctc
     2881 tgatcgaaaa acatcttctg gggggtcatc gccattgatc tcagctctga cgccagtgta
     2941 ggtcccgagg tgc
//
LOCUS       DASQGH010000625         4939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28233, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000625 DASQGH010000000
VERSION     DASQGH010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28233"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /gene="rimP"
                     /locus_tag="VEW91_12420"
     CDS             complement(<1..420)
                     /gene="rimP"
                     /locus_tag="VEW91_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYM92426.1"
                     /translation="MKRDEIAEQVTALTGPIATRLGLELIGVEIVGTGRHQVFRVLID
                     RDGGAGVEDCAQLSEALSRELDLYDLGRDPYTLEVASPGLDRTLRRLDDFIRFSGRAV
                     AITTSAPVDGQRRFRGRLLGLVDERIALRLEDGREIRL"
     gene            complement(583..1872)
                     /gene="asnS"
                     /locus_tag="VEW91_12425"
     CDS             complement(583..1872)
                     /gene="asnS"
                     /locus_tag="VEW91_12425"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HYM92427.1"
                     /translation="MLTTIDEIGRHVGTDVEVHGWVHNLRSSGAIHFLLLRDGTGILQ
                     AVAARQDLPPEVFAAAGGLTQESSVIAGGIVREDRRAPGGYELGLRRLTVVQTAGPYP
                     LTPKEHGVEFLMDHRHLWLRSSRPCAIMRVRAAVERAICDYLDDRGYVRMDTPILTPS
                     ACEGTSTLFETPYFERRAYLAQSGQLYNEATIAALGRVYCFGPTFRAEKSKTRRHLTE
                     FWMVEPEAAFLDLEGCIQLAEGLTVFVVQRVLERRRKDLETLERDLAALERVQAPFPR
                     LTYDEAVGILREQGHPIQWGEDFGGDEETALSQRFDRPVFVHRYPAQCKAFYMQPDPD
                     RPEVVLGADLLASEGYGEIIGGGQRIHDLDLLEQRLREARLPREEYAWYVDLRRYGSV
                     PHSGFGIGIERTVAWICGLDHVRETIPFPRLINRLYP"
     gene            1888..2652
                     /locus_tag="VEW91_12430"
     CDS             1888..2652
                     /locus_tag="VEW91_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYM92428.1"
                     /translation="MPPIGDGLGSLGYAGWGAFSSALADPGRRAYNARMIAPGRIALI
                     FDLDNTLIGSRIDFPAIRRSLIALLRGAGAAHEPDGELMRRPLAQLVAIGTAHDRARG
                     STLARQMWEIIEVHEVDGLRDAPPLDDAGDVLGVLRIRGYRIAILTNNSRAGALDALA
                     AAQLIGRAEVVVTRDDVRALKPAGDGVAEAARRLGQIDQLYMIGDSWIDGAAAAVSGA
                     RFIAYRRPIEELRDRGVEPWRTIMHLTELLDLDLTR"
     gene            complement(2672..3247)
                     /locus_tag="VEW91_12435"
     CDS             complement(2672..3247)
                     /locus_tag="VEW91_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92429.1"
                     /translation="MNKKREALRHGLRSFIEDTSRELADLEGRAPRPSPATPVPVDGV
                     APSNGAGHDAAPGPSTTVVAAPPAIAPPQAPPTPAARPAAAPRALPAPEPAPARAPRG
                     RGRQRRTQARPRLPQRVSPEGDEDGVNAAQAHARKGVCFAYFLNQACWRVPEAYCNSA
                     LQVCITRECPVYHLHKDALERRFAKKFQHFW"
     gene            complement(3210..4043)
                     /locus_tag="VEW91_12440"
     CDS             complement(3210..4043)
                     /locus_tag="VEW91_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYM92430.1"
                     /translation="MEGNVKPTARLIALVNQKGGCGKTTTAVNLAACLAINKRRVLLI
                     DLDPQANATVSLGVDPGQLTRTIYQVLVDEVLNGGTGVDLQSVIVPSNVANLDVAPAS
                     IDLAAAELELSARIGRENSLRKKVLPLRDRYHYIIIDTPPSLGLLTLNALVACEEIII
                     PIQTHYYALLGMRQLLRTLKVVREEAGHHVEIAGVVPTMYDARTSIGKEILEGIRDYF
                     GDKVFQSAIHFNIKLVESSMAGVPVFVHLPTSRGAHEYMNLAKEVMALEQKARSAAAR
                     A"
     gene            complement(4156..>4939)
                     /locus_tag="VEW91_12445"
     CDS             complement(4156..>4939)
                     /locus_tag="VEW91_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYM92431.1"
                     /translation="APRVAEVSPERIRDELILMVIGPDPGRALGLLHDTGLLAVLLPE
                     VAAEAGVPQPEAFHPEGDVFEHTRLALGQLHAPSVTLAMATLLHDVGKPPTLVQADRI
                     RFSRHDEVGAAIARSVMERLRFPRRDTDRVVAIVGRHMVFKDITKMREARLRRLVADE
                     VFPELLELHRADCAASHGDLSTYDWVRAYLDRLAGEPPIPPRLITGEDVVALGVPPGP
                     RVGWVLREVENAHLGGQIRTREEALALARALLATPGDAPSSR"
BASE COUNT          733 a         1737 c         1720 g          749 t
ORIGIN      
        1 caggcggatc tcccgcccgt cctccagcct cagcgcgatg cgctcgtcca cgagcccgag
       61 caggcgtcct cggaaccgcc gctgcccgtc gacgggggcc gacgtcgtga tggccaccgc
      121 ccggccggag aagcggatga agtcgtccag ccggcgcagc gtccggtcca gccccgggga
      181 agccacctcc agggtgtacg ggtcccggcc cagatcgtac aagtcgagct cccggctcaa
      241 cgcctcgctg agctgggcgc agtcctccac cccggccccc ccgtcgcggt cgatcaggac
      301 ccggaagacc tggtggcgcc ccgtcccgac gatctcgacg ccgatgagct cgaggcccag
      361 ccgcgtggcg atcggcccgg tcagggcggt cacctgctct gcaatctcgt cgcgtttcat
      421 ctgcttcgcc cctacaaacg tgcgccccag cctttggcgc cctccaagca acgaaagagt
      481 gggtcgggca cccactcccc cttcgaaagc gccaagattg gggcacagaa ttacaaacgc
      541 acttgtagta tagcacacgg ctgcgaaaag aaaaggcgcg gctcagggat agaggcggtt
      601 gatcagccgg ggaaacggga tcgtttcccg gacgtggtcg agcccgcaga tccaggcgac
      661 cgtccgctcg atcccgatcc cgaacccgct gtggggcacc gacccgtagc gccggaggtc
      721 gacgtaccac gcgtactctt cccggggaag acgcgcctcg cgcagccgct gctcgagcag
      781 gtcgagatcg tggatccgct gcccgccccc gatgatctcg ccgtagccct ccgatgccag
      841 gaggtccgcg ccgagcacga cctcggggcg gtcggggtcg ggctgcatgt agaacgcctt
      901 gcactgcgcg gggtaccgat ggacaaacac cggccggtcg aagcgctggc tcagcgcggt
      961 ctcttcatcg cccccgaaat cctcccccca ctggatcgga tgtccctgct cccggaggat
     1021 ccccaccgcc tcgtcgtagg tcagccgggg aaacggcgcc tggacgcgct cgagcgcggc
     1081 gagatcccgc tccagcgtct cgagatcctt gcgacggcgc tcgagcaccc gctggaccac
     1141 gaacaccgtc aggccctccg ccagctggat gcacccttcg agatcgagga acgccgcctc
     1201 aggctcgacc atccagaact cggtcaggtg ccgccgggtc ttgctcttct cggcccgaaa
     1261 cgtcgggccg aagcagtaca cgcgcccgag cgcggcgatg gtggcctcgt tgtagagctg
     1321 cccgctctgc gccaggtagg cccggcgctc gaagtacggg gtctcgaaca gcgtgctcgt
     1381 cccctcgcag gcggaggggg tgagaatcgg ggtgtccatc cggacgtatc cgcggtcatc
     1441 gaggtagtcg caaatcgccc gctccaccgc tgcccgcacc cgcatgatgg cgcacggacg
     1501 gctgctgcgg agccagaggt gccggtgatc catgaggaac tccacgccgt gctccttggg
     1561 cgtgagcggg tacggcccgg cggtctgcac gaccgtgagg cgcctgaggc cgagttcata
     1621 cccccccggg gcccgccggt cctcgcggac gatccccccg gcgatcaccg aggactcctg
     1681 ggtgaggccg ccggcggccg caaagacctc cggcggcagg tcctggcgcg ccgccaccgc
     1741 ctggaggatc ccggtcccat ccctcagcag gagaaagtgg atcgccccgc tgctgcggag
     1801 gttgtgcacc cagccgtgca cctcgacgtc ggtgccaacg tgccggccga tctcatcgat
     1861 cgtggtgagc atcatcggtg ccgcgcgatg cctcccatcg gggatggtct gggcagccta
     1921 ggctacgccg ggtggggagc cttctcctct gcgcttgcgg acccggggcg gcgggcgtac
     1981 aatgcgcgta tgatcgcgcc cggccggatc gcgctcatct tcgatctgga caacaccttg
     2041 atcgggtcgc ggatcgactt ccccgcgatc cgccggtccc tgatcgcgct gctccgcggt
     2101 gcgggggcgg cgcatgagcc ggatggcgag ctgatgcgcc ggccgctcgc gcagctcgtc
     2161 gcgatcggca ccgcgcacga ccgcgcgcgc ggctcaaccc tcgcccgcca gatgtgggag
     2221 atcatcgaag tccacgaggt cgatgggctc cgcgatgcgc cgccgctcga cgacgccggg
     2281 gacgtgctcg gggtcttgcg catccgaggg tatcgcatcg ccatcctcac caacaacagc
     2341 cgggcggggg ccctggatgc gctggcggcc gcccagttga tcggccgcgc cgaggtggtg
     2401 gtcacccggg acgacgtgcg cgcactcaag ccggccgggg acggggtcgc cgaggccgcc
     2461 cgccggctcg gacagatcga ccagctctat atgatcggag actcgtggat cgacggggct
     2521 gccgcggccg tctccggcgc caggttcatc gcctaccggc gccccatcga ggagctgcgc
     2581 gatcgcgggg tcgaaccgtg gcgcacgatc atgcacctga cggagttgct ggatctcgat
     2641 ctgacccggt agccggcagg ccgcggcacg gctaccagaa gtgctgaaac ttcttcgcga
     2701 accgccgttc cagcgcgtcc ttgtgcaggt ggtacacggg gcactcccgg gtgatgcaga
     2761 cctggagggc gctgttgcag taggcttcgg gaacccgcca gcaggcctgg ttgaggaagt
     2821 acgcaaagca cacgccctta cgcgcgtggg cctgggctgc gttcaccccg tcctcgtcac
     2881 cctcgggtga cacgcgttgg ggcaggcgcg ggcgtgcctg ggtgcggcgc tggcgccctc
     2941 ggccccgggg cgcgcgcgcc ggggccggtt caggcgcggg cagcgctctc ggggccgcgg
     3001 ccggccgcgc cgccggcgtg gggggggcct gcggaggcgc gatggccgga ggcgcagcca
     3061 cgacggtcgt ggaggggccg ggggccgcgt cgtgcccggc gccgttgctc ggggcaaccc
     3121 catccaccgg gaccggggtg gccggcgagg gtcggggcgc gcggccttcc aggtcggcga
     3181 gttcgcgcga cgtatcttcg atgaacgatc taagcccgtg ccgcagcgct tctcgctttt
     3241 tgttcaagcg ccatgacctc cttggcgagg ttcatgtact cgtgcgcccc gcgagaggtg
     3301 ggcaggtgga cgaacaccgg cacgccggcc atgctggact ccacgagctt gatgttgaag
     3361 tggatggcgc tctggaacac tttgtccccg aagtagtcgc ggatcccttc gaggatctcc
     3421 ttgccgatgc tcgtccgggc gtcgtacatc gtcggcacga ccccggcgat ctcgacgtgg
     3481 tggccggcct cctcccggac gaccttgagg gtccgcagca gctgccgcat ccccagcaac
     3541 gcgtaatagt gggtctggat cgggatgatg atctcctcgc aggccacgag cgcgttgagg
     3601 gtgagcagtc cgagcgacgg gggcgtgtcg atgatgatgt agtggtaccg gtcgcgcagg
     3661 ggcaggacct tcttgcgcag cgagttctcc cgcccgatcc gcgccgacaa ctcgagttcc
     3721 gcggcggcca ggtcgatcga ggcgggggcc acatcgaggt tcgcgacgtt ggagggcacg
     3781 atgacgctct ggagatccac gcccgtaccg ccgttcagga cttcatcgac gagcacctgg
     3841 tagatggtcc gggtgagctg gcccggatcg accccgaggc tcaccgtggc gttggcctgc
     3901 gggtcgaggt cgatgagcag cacgcggcgt ttgttgatag caaggcaggc ggccaggttg
     3961 accgccgtcg tcgtctttcc gcacccgccc ttctggttga ccagagcgat caggcgggcc
     4021 gtgggcttca cgttcccttc cacggcgctc ctcccgcacg aaccccggtg ggcatcagcg
     4081 ggcaggccga cgcgcgctca cccgcgatcg tacccatgtt cgactctcgg cgcggaatct
     4141 ccttcgcgcc cccgttcacc gcgatgacgg cgcatctccg ggggtggcca ggagcgcgcg
     4201 ggccaacgcc agcgcctcct ctcgggtccg gatctggccc ccgaggtgcg cgttctcgac
     4261 ctcacggagc acccacccca cccgggggcc cgggggcact ccgagcgcca ccacatcctc
     4321 gccggtgatg agccgcggcg gtatcggcgg ctccccggcc aggcgatcga ggtaggcccg
     4381 gacccagtcg tacgtcgaga ggtccccgtg gctcgcggcg cagtcggccc ggtgcagctc
     4441 caggagctca ggaaacacct catcggccac gagccggcgc agccgggctt cccgcatctt
     4501 cgtgatgtcc ttgaacacca tgtggcgtcc gacaatggcg accacgcgat cggtgtcgcg
     4561 ccgcgggaac cggagccgct ccatcacgga ccgagcgatc gccgctccga cttcgtcgtg
     4621 gcgggagaac cgaatccggt cggcctgcac cagcgtgggg ggcttcccga catcgtgcag
     4681 cagggtggcc atcgccaggg tgaccgaggg agcgtgcagc tggcccaagg ccaggcgggt
     4741 gtgctcgaag acatccccct cggggtggaa cgcctcgggc tggggcacgc cggcctcggc
     4801 cgccacctcg gggaggagga cggcaagcaa tccggtgtca tgcagcagcc cgagcgcgcg
     4861 gccggggtcg ggaccgatca ccatcaggat cagctcgtcg cgaatccgct ccgggctcac
     4921 ctccgcgacg cggggcgcg
//
LOCUS       DASQGH010000626         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50940, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000626 DASQGH010000000
VERSION     DASQGH010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50940"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VEW91_12450"
     CDS             <1..342
                     /locus_tag="VEW91_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HYM92432.1"
                     /translation="DRVKDVIKSGGEWISSVDLENTIMAHPAVAEAAVVGVEHPKWQE
                     RPLACVVPKPGKAPTKDEILALLKEKFASWWIPDDVVFIDEVPKTSVGKFDKKVLRER
                     FAGHLLKQAVP"
     gene            complement(361..972)
                     /gene="leuD"
                     /locus_tag="VEW91_12455"
     CDS             complement(361..972)
                     /gene="leuD"
                     /locus_tag="VEW91_12455"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYM92433.1"
                     /translation="MEPFRTHTGVVVPFDRTDVDTDLIIPARYLKRIDRAGYGQLLFY
                     SKRFLPDGAPDPEFVLNRPQYRGSTVLVAGRNFGCGSSREHAAWALQDYGFKVIVAPS
                     FADIFANNAAQIGLLTIVLPEERVRQIMEVAGTREGYQLTVDLEAQTVTDGFGHRDRF
                     EVDAFKKHCLLNGLDAIGLTLQHEAEIARFEAARPAWMPRVAR"
     gene            complement(978..2384)
                     /gene="leuC"
                     /locus_tag="VEW91_12460"
     CDS             complement(978..2384)
                     /gene="leuC"
                     /locus_tag="VEW91_12460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HYM92434.1"
                     /translation="MSPKTSIDKIWDAHAVRSRPGEPDLLYVDLHLVHEVTSPQAFDG
                     LRLHHRRVRRPDLTFATVDHNVPTTPRTEPVTDPVSRLQIEALERNCREFGIHLEGML
                     SQRQGIVHIIGPELGLTQPGMLIVCGDSHTATHGALGALAFGIGTSEVEHVLASQILP
                     QRRPKTMEVRVDGALPLGTTPKDLILGVIRRYGVGGGIGHIIEYTGETIRGLSMEGRL
                     TVCNMTIEGGARAGLIAPDETTFAYLEGRPYAPKGKAWDEAVAFWKRLPTDPGAAYDT
                     RVALDAATLEPYVTWGTNPSQSEPVTGRVPDPSAQADPQARSAVERALQYMALRPGTP
                     IQEIPIDRVFIGSCTNARLEDLRVAARVVAGRKVHPKVRAMIVPGSQQVKSAAEREGL
                     DRVFTGAGFEWREAGCSMCLGMNPDVLGSGERCASTSNRNFEGRQGPGGRTHLVSPPM
                     AAAAAIAGHFVDIREWRV"
     gene            complement(2381..>2607)
                     /locus_tag="VEW91_12465"
     CDS             complement(2381..>2607)
                     /locus_tag="VEW91_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HYM92435.1"
                     /translation="VEIHLDVGDRGETKRIIREVVGMVQGSGFAAITKPDAYGASKVA
                     DRETGKLRVGERARGAGGGHPPSPTRPGGA"
BASE COUNT          379 a          943 c          883 g          402 t
ORIGIN      
        1 gatcgggtga aagacgtcat caagtcaggg ggggagtgga tctcctcggt cgacctggag
       61 aacaccatca tggcccaccc ggcggtcgcg gaggccgcgg tggtgggggt cgagcatccc
      121 aagtggcagg aacggcccct cgcgtgcgtc gtcccgaagc cggggaaggc ccccaccaaa
      181 gacgagatcc tcgccctgct caaggaaaag ttcgcctcct ggtggatccc cgatgacgtg
      241 gtctttatcg acgaggtgcc gaagaccagc gtcgggaagt tcgacaagaa agtgctgcgg
      301 gagcggttcg cgggccacct gctcaaacag gctgtgccat aacccccgct gcggcggggc
      361 ctaccgagcc acccgcggca tccacgccgg gcgggcggcc tcgaaccggg cgatctcggc
      421 ctcgtgctgc agcgtcaggc cgatcgcgtc caacccgttg agcaggcagt gcttcttgaa
      481 cgcgtcgacc tcgaaccggt cgcggtgtcc aaacccatcc gtcaccgtct gggcctcgag
      541 gtccacggtc agctggtacc cttcccgcgt gccggccacc tccatgatct gccggacccg
      601 ctcttcaggc aggacgatgg tcaagagccc gatctgcgcc gcgttgttgg cgaagatgtc
      661 cgcgaacgac ggggcgacga tgaccttgaa tccgtagtcc tgcagcgccc aggccgcgtg
      721 ctcgcgggag gagccgcacc cgaagttgcg gcccgcgacc aggacggtgc tcccgcggta
      781 ctggggccgg ttcaaaacga actccgggtc gggcgcaccg tccgggagga agcgcttgct
      841 gtagaagagc agctgcccgt accccgcgcg atcgatccgc ttgagatagc gcgcggggat
      901 gatgaggtcc gtgtcgacat ccgtccggtc gaacggcacc acgacgccgg tatgagttct
      961 gaagggctcc atcccggcta cacccgccac tcccggatgt cgacgaagtg cccggcgatc
     1021 gccgccgccg ccgccatcgg ggggctcacc aggtgcgtcc ggccgccggg gccctgccgg
     1081 ccctcgaagt tccggttcga ggtcgacgcg cagcgctccc cggatccgag cacgtcgggg
     1141 ttcatcccca gacacatcga gcagccggcc tcgcgccact cgaacccggc gccggtaaag
     1201 acgcggtcga gcccctcccg ctccgccgcc gacttgacct gctgggatcc cggcacgatc
     1261 atcgcccgca ccttgggatg gaccttccgg ccggccacca cgcgcgccgc cacccggagg
     1321 tcctcgagcc gcgcgttcgt gcacgagccg atgaagacgc ggtcgatcgg gatctcctgg
     1381 atcggggtcc cgggcctgag ggccatatac tgcagcgcgc gctccaccgc gctccgcgcc
     1441 tgcggatcgg cctgcgcgct cggatccggc acgcgccccg tgaccggctc cgactgcgac
     1501 gggttggtgc cccaggtcac atacggctcg agggtcgcgg cgtccagcgc gacgcgcgta
     1561 tcgtaggccg cgccgggatc ggtcggcaga cgcttccaga aggccaccgc ctcgtcccac
     1621 gctttgccct tgggggcgta cgggcggccc tcgaggtagg cgaaggtcgt ctcgtccggg
     1681 gcgatcagcc cggcccgggc ccctccctcg atcgtcatgt tgcacacggt gaggcggccc
     1741 tccatcgaga ggccgcggat cgtctctccg gtgtactcga tgatgtggcc gatcccgccg
     1801 cccacaccgt accgccggat cacgccgagg atcaggtcct tgggcgtggt gccgagcggc
     1861 agggcgccgt cgacccgcac ctccatcgtc ttcgggcgcc gctgcggcag gatctggctc
     1921 gccagcacgt gctccacctc ggaggtcccg atcccgaacg ccagcgcgcc gagcgccccg
     1981 tgagtggccg tgtggctgtc cccgcagacg atcagcatcc ccggctgggt gagcccgagt
     2041 tccggcccga tgatgtggac gatgccctgc cgctggctca gcatgccttc gaggtggatc
     2101 ccgaactcgc ggcagttccg ctccagggcc tcgatctgca accggctcac cggatcggtc
     2161 accggctcgg tccgcggcgt ggtcggcacg ttatggtcca ccgtcgcgaa cgtaaggtcc
     2221 ggccggcgca cgcggcggtg gtggagacgc aggccgtcga acgcctgcgg ggacgtcacc
     2281 tcgtgcacca ggtgcaggtc cacgtagagc aggtcgggct caccgggccg gctccgcacg
     2341 gcgtgggcat cccagatctt gtcgatcgac gtcttcgggc tcatgcgccc cccggccgcg
     2401 ttggactcgg ggggtggcct ccgccggctc ccctggcccg ctcaccgacg cggagcttgc
     2461 cggtctcccg gtccgccacc ttgctcgcgc cgtaggcatc gggcttggtg atcgccgcga
     2521 agccgctgcc ctggaccatc ccgacgacct cgcggatgat gcgcttggtc tccccgcggt
     2581 cgccgacatc gaggtggatc tccaccg
//
LOCUS       DASQGH010000627         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11197, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000627 DASQGH010000000
VERSION     DASQGH010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11197"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VEW91_12470"
     CDS             <1..466
                     /locus_tag="VEW91_12470"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYM92436.1"
                     /translation="LEEDEAYWRRVIDTNLWGVIFCSRVALEWMVEHSGGVVVNVASD
                     AGRVGSSGEAVYSAAKGGVIALTRALAREMASHKIRVNCVCPGPTETAVLVENRADRA
                     QAARIERMIRLIPMRRVAQPEEVAEAVAFFCTDASRYITGQVLSVSGGLTMA"
     gene            466..1518
                     /gene="plsX"
                     /locus_tag="VEW91_12475"
     CDS             466..1518
                     /gene="plsX"
                     /locus_tag="VEW91_12475"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HYM92437.1"
                     /translation="MASLGRPPRIAVDCMGGDHAPKEIVAGALAAADAERLEILLVGT
                     PQALTPLVPTGILQSGRAEIVPSAGAIPEGAPPLRAVRASPNASMAVAMRQVGTGRAD
                     AAVSAGSTGPTLLAAVREIGLLEGLRRACVGRQILELHPETFLIDLGPTMDCEARHLV
                     EFAVIGATYMRVFCGIPDPSVALLSNGREPGKGNRVVKDAAALLERSGLRFAGLVEGH
                     DLMRGAANVVVCDGFVGNVALKVVEGLGHEVAAWLRRELAGTVPAERAARIADRVVEM
                     TNPIDLHGSLPLLGIRGNVVMAHGASDRRAIAAAIAQAVQAVRGGFVERLRTALAETR
                     ERLNRPVNARQRLCDS"
BASE COUNT          253 a          583 c          644 g          275 t
ORIGIN      
        1 tcttgaggag gacgaggcct actggcgccg cgtgatcgac accaacctgt ggggcgtgat
       61 cttctgctcg cgcgtggcgc tcgagtggat ggtggagcac agcggcggag tggtcgtcaa
      121 cgtcgcgagc gacgccgggc gggtcgggtc cagcggcgag gctgtgtact cggcggccaa
      181 gggcggagtc atcgcgctca cgagagcgct cgcgcgagaa atggcctccc acaagatccg
      241 ggtcaactgc gtctgcccgg gcccgacgga gaccgcggtc ctggtcgaga accgtgccga
      301 ccgtgcgcag gccgcgcgca tcgagcggat gatccgcctg atcccgatgc gccgcgtcgc
      361 gcagcccgag gaggtcgcgg aggcggtggc gttcttctgc accgacgcct cgcggtacat
      421 tacgggacag gtgctgagcg tctccggcgg gctcacgatg gcgtgatggc ctccctcggt
      481 cgcccgccgc gcatcgccgt cgactgcatg ggcggtgatc acgctccgaa agagattgtc
      541 gccggggcgc tggccgcggc cgatgcggag cgccttgaga tcctcctcgt cggcaccccg
      601 caggccctca cccccctcgt gccaacgggc atcctccaat ccgggcgggc ggagatcgtc
      661 ccgagcgcgg gggcaatccc cgagggggcc ccgccgcttc gggcggtccg ggcctccccc
      721 aacgcgtcca tggcggtggc gatgcggcag gtcgggacgg ggcgggcgga cgctgcggtg
      781 agcgccggca gcacggggcc gacgttgctg gccgccgtgc gggagatcgg cctgctggag
      841 gggctccggc gggcctgcgt cggccggcag atcctggaac tgcacccgga gacgtttctc
      901 atcgacctgg ggccgacgat ggactgcgag gcccggcacc tcgtcgagtt cgccgtgatc
      961 ggcgccacat acatgcgggt gttctgcggg atccccgacc cctcggtcgc gctcctcagc
     1021 aacggccgcg agccgggcaa gggcaaccgg gtcgtgaagg acgcggcggc gctgctcgaa
     1081 cgcagcggcc tccggttcgc ggggctcgtc gaggggcacg acctgatgcg cggggccgcc
     1141 aacgtggtgg tgtgcgacgg gttcgtgggc aacgtcgcgt tgaaggtcgt cgaagggctg
     1201 gggcacgagg tcgcggcgtg gctccggcgc gagctcgccg gcacggttcc ggccgagcgc
     1261 gccgcgcgga tcgccgaccg ggtggtggag atgacgaacc ccatcgacct ccacggaagc
     1321 ctccccctcc tggggatccg gggcaacgtc gtcatggcgc acggcgcgtc ggaccgccgc
     1381 gcgattgcgg cggcgatcgc gcaggcggtg caggccgtcc ggggcggatt tgtcgaacgg
     1441 ctgcggactg cgctggccga gacccgcgaa cgcctcaacc gcccggtgaa cgcccgtcag
     1501 cgattgtgtg attcatgagt agcttcaccc tccccaccag ttcgtggagg ctcttacggg
     1561 agctcctcag gattgccgca tatcccgtct tagtgacaat ttcggattta agcccgaatt
     1621 tccaagactc acaccccatt gcttgtaagc gtcattcgcg gcgcagaatt gtgtttggca
     1681 actcctattc ttcgatggca ccaccaatcc ggcgcaggtg ctcgcgaagg gttgccgccg
     1741 gattggaaga acgcc
//
LOCUS       DASQGH010000628         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_50950, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000628 DASQGH010000000
VERSION     DASQGH010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50950"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..428)
                     /locus_tag="VEW91_12480"
     CDS             complement(207..428)
                     /locus_tag="VEW91_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92438.1"
                     /translation="MTVQDRQDQSLEISIQARLCNSSRYHGVGDLVTYRSGVWVVKGY
                     YFGPAQSGERELYYILSEATAPAHQFDSV"
     gene            complement(472..1128)
                     /locus_tag="VEW91_12485"
     CDS             complement(472..1128)
                     /locus_tag="VEW91_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM92439.1"
                     /translation="MGAVRVMIVDDMTLVRAGIGSLLKGRDAIGLVGEAADIREAIGA
                     LAQADPDVVLLDQDMPGLGTPEAVGLLKQHRPQTEILVLSETPDEERAFRALEAGASG
                     FILKDITPDNLVRAIHGVCDGRTTTHPYITRRLIERFRVLMAERSHKNGTRPAGLTNR
                     ELEIVVEMAEGATDREIAQKAHLGETTVKSHIRSILRKIRARNRTQAVAYLLRNGVMR
                     "
     gene            1348..2001
                     /locus_tag="VEW91_12490"
     CDS             1348..2001
                     /locus_tag="VEW91_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYM92440.1"
                     /translation="MPVRVMLVDDHEIITAGLRVVLQAEHDIAVVGKASGVKEAVRKA
                     AELKPDVVLMDVKLSDGSGIDATKQIKEASPETQVLILTVYDDQDTVLKAVQAGAIGY
                     VLKDIPPENLIRAIRSVHADRTMINPAIARKLVERLATTEREAVLFNFRRGPGLTDRE
                     IEVLKGVASGLSDKEIALKLFLSEPTVKSHLRSVYQKLRIHNRAQAAAYAVKNGLTV"
     gene            2080..>3300
                     /locus_tag="VEW91_12495"
     CDS             2080..>3300
                     /locus_tag="VEW91_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYM92441.1"
                     /translation="MPILESRVDARSPEFAANRAYHEGLAKTLAERLAKVRAGGGERA
                     QRRHREQGKKTARERIDLLLDPASPFLEIAPLAAWGMYGGEAPSGGIVTGIGRVRGLE
                     CMIVANDATVKGGTYYPLTVKKHLRAQEIALENHLPCLYLVDSGGAFLPLQAEVFPDR
                     DHFGRIFYNQAQLSSRGIPQIAAVLGSCTAGGAYVPAMADEAVIVRGTGTVFLGGPPL
                     VRAATGEEVTAEELGGADVHSRRSGVTDYYAEDDAHAISILREIVGSLNRVKRPGVAL
                     EPPEDPRYDPREIYGILPQDLRRQFDAREIIARLADGSRFQEFKALYAPTLVTGFARI
                     HGIPVGILANNGVLFVESAEKGAHFIELCGQRRIPLLFLQNITGFIVGRQYEAAGIAR
                     AGAKMVHAVATAGVP"
BASE COUNT          556 a         1120 c         1104 g          520 t
ORIGIN      
        1 caggaaggcg aggcagcata caccaacacc gcggccgagg tgcaggcgaa cgccgcgcag
       61 ccgaccaaaa ccgctgtaac ccgtcttcgc atgcatatcc tcctcaaatc cttcatcctg
      121 gatcatggca cgccacccca aagatccgct atccctcttt atgatgaagg ggtgcgatcg
      181 gccgatagga accgaggaag ccgtcctcag acgctatcga actggtgggc aggagccgtg
      241 gcttcggaca ggatgtagta gagttcgcgc tctcccgatt gggccgggcc gaagtagtat
      301 cccttcacca cccacacccc ggaccggtac gtgaccagat ctcccacgcc gtggtagcgg
      361 gagctgttgc acagcctcgc ctggatgctg atttctaggg actgatcttg gcgatcctgt
      421 acggtcacct ggacacctcc caagcctcgg gcgtcccgag catcccgcat tctaccgcat
      481 gaccccattg cgcaagagat aggcgaccgc ctgggtccgg ttgcgggcgc ggatcttccg
      541 gaggatcgac cggatgtggc tcttgaccgt ggtctcgcct aggtgcgcct tctgggcgat
      601 ctcgcggtcc gtggccccct cggccatctc gacgacgatc tccagttccc ggttggtcag
      661 gcctgcgggg cgcgtcccgt tcttgtggct gcgctccgcc atcaggaccc ggaatcgttc
      721 gatgaggcgg cgcgtgatgt agggatgggt cgtcgtccgg ccatcgcaga ccccgtggat
      781 cgcgcgcacc aggttgtcgg gggtgatgtc cttgaggatg aagccgctgg ctcccgcctc
      841 gagcgcgcgg aacgcccgct cttcgtccgg ggtctccgac agcacgagga tctccgtctg
      901 cgggcggtgc tgcttcagca gaccgacggc ctcgggggtg ccgagcccgg gcatgtcctg
      961 atcgagcagc acgacatcgg gatcggcctg agccaacgcc ccgatagctt ctcggatgtc
     1021 tgccgcttcc ccgacgagcc cgatggcgtc tcgccctttg agcaggctcc cgatcccggc
     1081 ccgcacgagc gtcatatcgt ctacgatcat cacgcgaacg gcacccacgt ttgcctcccc
     1141 cgcatagccc ttgcgtcggt gtgagttacc taagagaata ggcagaccct tggttcatct
     1201 ggggggatta gtccgtcgtt cgaaggatag gggttcatcc gaacggcggg ggtccccggg
     1261 gcggggctac cccaggaccg aacggggcat atatctttaa tgtacgcagg cccaggtccg
     1321 aaccacgttc gaggaggcga cccaagggtg cccgtccgcg tcatgctggt ggacgatcac
     1381 gagatcatca ccgccggcct ccgggtggtc ctgcaggcgg agcacgacat cgccgtagta
     1441 ggaaaggcct ccggggtcaa ggaggcggtc cgcaaggcgg ccgagctcaa gccggacgtg
     1501 gtgctgatgg acgtcaagct ctcggacggc agcgggatcg acgcgaccaa gcagatcaag
     1561 gaggcctccc ccgaaaccca ggtgctcatc ctcacggtgt acgacgacca ggacaccgtg
     1621 ctcaaagcgg tccaggccgg ggcgatcggg tatgtcctga aggacatccc gcccgagaat
     1681 ctcatccgcg ccatccggtc ggtccacgcc gaccgcacca tgatcaaccc ggcgatcgcc
     1741 cggaagctcg tcgagcgcct ggcgaccaca gagcgggagg cggtgctgtt caacttccgc
     1801 cgcgggcccg gcctcaccga tcgggagatc gaggtgctga agggcgtcgc gtccgggttg
     1861 agcgacaaag agatcgcgct gaagctcttc ctctcggagc ccacggtcaa gagccacctc
     1921 cgctcggttt atcagaagct ccgcattcac aaccgcgcgc aggccgcagc ctacgccgtc
     1981 aagaacggcc tcacggtcta acggacctcc ccgcaagatc gagcccgcgc gcccgctgaa
     2041 agaggggcgg ggcgcggcta ccgggaatac ccccgggcga tgccgatcct cgagtcccgg
     2101 gtcgatgccc ggtccccgga gttcgccgcc aaccgcgcat accacgaagg cctcgccaag
     2161 accctcgccg agcggctcgc caaggtccgc gcgggcgggg gggaacgcgc ccagcggcgg
     2221 caccgggagc aggggaagaa gacggccaga gagcggatcg acctgctgct cgaccccgcc
     2281 tccccgtttc tggagatcgc cccgctggcc gcgtggggga tgtacggcgg cgaggcgccg
     2341 tccgggggca tcgtcaccgg gatcggccgc gtccgcgggc tcgagtgcat gatcgtcgcg
     2401 aacgacgcga ccgtcaaggg cgggacctac taccccctga cggtgaagaa gcacctgcgc
     2461 gcacaagaga tcgccctcga gaaccacctc ccgtgtctct acctcgtgga cagtggcggt
     2521 gcgttcctgc cgctccaggc cgaggtcttt cccgaccgcg accacttcgg gcgcatcttt
     2581 tataatcagg cgcagctgtc gtcccgcggc atcccgcaga tcgcggccgt gctgggctcg
     2641 tgcaccgcgg gcggcgcgta cgtgccggcg atggccgacg aggcggtgat cgtccgcggg
     2701 accgggacgg tgttcctggg gggcccgccg ctcgtgcggg cggcgaccgg ggaggaggtc
     2761 accgcggaag agctgggcgg ggccgacgtg cactcccgcc gcagcggcgt cacggattat
     2821 tacgccgagg acgacgcgca tgcgatcagc atcctcaggg agatcgtcgg ctcgctcaac
     2881 cgcgtcaagc ggcccggcgt ggccctggag ccgccggagg acccccggta cgacccgcgg
     2941 gagatctacg ggatcctccc gcaggacctc aggcggcagt tcgatgcccg cgagatcatc
     3001 gcccgcctgg cggacggcag ccggttccag gagttcaagg cgctgtacgc gcccacgctg
     3061 gtgaccggct tcgcccgcat ccacgggatc ccggtgggga ttttggcgaa caacggcgtc
     3121 ctgttcgtgg agagcgccga gaaaggcgcg cacttcatcg agctgtgcgg ccagcgccgg
     3181 atcccgctcc tcttcctcca gaacatcacc gggttcatcg tcggccggca gtacgaagcc
     3241 gccgggatcg cgcgggcggg ggcgaagatg gtgcacgccg tcgcgacggc cggggtgccc
//
LOCUS       DASQGH010000629         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_28250, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000629 DASQGH010000000
VERSION     DASQGH010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28250"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VEW91_12500"
     CDS             complement(<1..835)
                     /locus_tag="VEW91_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYM92442.1"
                     /translation="MMKFPWRVVLPRIGSRGGTVRRPFRWSVPTLWPYLAWASVAGLA
                     VVLALTGVLAGAALAISGHLPRVDALYSPPSEATRIYAADGQLIASLYQENRASVALA
                     DIPETLRRAVIDTEDAAFYRHPGISLRGVLRASFRNVRERGLAEGGSTITQQLARNLF
                     LTNEKALGRKIAEMLLAIQIERRLTKDEILERYLNQVYFGQGTYGVEAAAEVYFGQPV
                     KALALPESALLAGLIRAPSYYSPYDHMDRARIRRAEVLQRMLDLWDITPQHMRTADSA
                     PI"
     gene            1710..2621
                     /locus_tag="VEW91_12505"
     CDS             1710..2621
                     /locus_tag="VEW91_12505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM92443.1"
                     /translation="MDAATGCPALPPPYDGPIFDANVQAWSPNIQVLIENIAGSGVRR
                     IALFVNSGQGGEATVSAVLAAHQEHPELIVVGVPKIGFIVGGDLPASYVSSTVAGIAD
                     GTYTFIGEILYTHGDKPDHKPTRNGQVYVDPLASGTAHLLDGIAGHTTPLLTHWEAWA
                     WDRDWPHFDRLYASRPQQRFVLPSLAYGSPEQADGILSAHPNVWGIISRLVDGRYRFV
                     DPAKQARLGPPMFDACGALRPEWRAVLTKHRDRLMYGSDDYSTARVGWNAYPGTIDKY
                     RRIAGQLPPDLAREISWDNAAALYGGR"
     gene            complement(2653..3432)
                     /locus_tag="VEW91_12510"
     CDS             complement(2653..3432)
                     /locus_tag="VEW91_12510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYM92444.1"
                     /translation="MAGPRGHLVGWTLVAAILLAVLVSPGAATSSSVLVGVRHNMRAN
                     VITPDGHGPYPGVLVLHTAGGLEPADLDYAQRLAREGYVCLVPAFMEAYRISGPSRDA
                     DIVAHASELYADFVDALAVLRRNEKVGGSRVGAVGFSAGGGFATWLAATSKIQAGVSY
                     YGGFRPGTDLSLSQMQAAFTSTSAPVLILQGSADAPATVRVAERLRAILDRAHSPYEI
                     YFYPGAGHTFERELASKANRDAAEDSWRRTLEFLGKYLKQP"
BASE COUNT          614 a         1214 c         1231 g          610 t
ORIGIN      
        1 ggatcggagc gctatccgcg gtccgcatgt gctgtggggt gatgtcccac agatccagca
       61 tccgctgcag cacctcggcc cggcggatcc tggcgcgatc catgtggtcg tagggggagt
      121 aataggacgg cgccctgatc aaccccgcga ggagcgcgct ttcgggaagc gcgagcgcct
      181 tcaccggctg accgaagtac acttccgcgg ccgcctccac cccgtaggtc ccctggccaa
      241 agtacacctg gttgaggtac cgctccagga tctcatcctt cgtcagccgg cgctcgatct
      301 ggatcgccag cagcatctcg gcgatcttcc ggccgagcgc cttctcattc gtgaggaaga
      361 ggttgcgggc cagctgctgg gtgatcgtgc tgccgccctc ggccagcccc cgctcgcgaa
      421 cgttccggaa cgacgcccgg agcacgcccc ggagcgagat cccggggtgc cggtagaacg
      481 cggcgtcctc ggtgtcgatc acggcgcgcc ggagggtctc gggaatgtcc gcaagcgcca
      541 cgctcgcgcg attttcctga tagaggctcg cgatgagctg gccatcggcc gcgtagatgc
      601 gggtggcctc gctcgggggg ctgtagaggg cgtcaacccg cggcaggtgg ccgctgatcg
      661 ccagggcggc tcccgcgagc accccggtga gggccagcac cacggccagc cctgcgacgg
      721 acgcccaggc aagatacggc cagagggtgg ggacggacca ccggaagggc ctccgcacgg
      781 tcccgccgcg gcttccgatg cggggcagga ccacacgcca tgggaacttc atcatcgagg
      841 gcccctggct tcgatggctg cgttgatgac tgcgttaacg agagtgtcac gcatgctcgg
      901 tctcagaatt gagtataccc atctcgggaa aagccagcaa gacggagttc ccgggcgcgc
      961 cggaggtcga gtccggcggg acgcgacggt tcccgagggc aggtttcaac cgcggccgag
     1021 gagggtaata ggtatggcgg gatggcgtgg gggacacctc atcggagttc ccccccttgc
     1081 cgatcggggt gatcccccgc gacatcctgg ttgcaacttg tcatcattgc gggcctgtgg
     1141 gctgggaccc cggcgcgcac cgagcccacg gctggcgcgc ggggcgcggg tccgcaaagg
     1201 agggatcgca gatgctggtg gcgttcctgc tgctcgcagg ccttctgctg tgggcgaccg
     1261 gccatatgat cctccggtaa cgcatccaca gcgcacacca ccgtgcaacg cgccgggccg
     1321 ggcagttccc ggcccgccgc gttttggggc ggtgcccaac ccaggcctac agagcgagat
     1381 taacgcggaa acggcagctt ttctcctcca ggaatgggct cctgtcgagg ccaccaatgg
     1441 tgtgtcgtca gccctcgagc accagaccct gagtgaactt gttggacgtg ccgtgcgtcg
     1501 ctacgtgcgg gcggtggcga agggatggcc gcaggaatac tttgcacatc ttccgcgcct
     1561 tgggactcag actccgaaag acgcggttca ctggatgcgt gccaacaaag gatgtaaccg
     1621 aaaaccatcg aacggggaaa cttgggatgc gctggttcgt cggtctggcg ttcacgttgt
     1681 ggtgtgcgat gctcgcgttc ggcggtctcg tggacgccgc cacgggatgc ccggcgctgc
     1741 cgccgcccta cgacggcccg atcttcgatg ccaacgtgca ggcgtggagc ccgaatatcc
     1801 aggtcctgat cgagaacatc gcgggatcgg gcgtccggcg catcgcgctg ttcgtgaaca
     1861 gcgggcaggg cggcgaggcg accgtctcag ccgtgctggc ggcccaccag gaacatccgg
     1921 aactcatcgt ggtgggcgtg ccaaagatcg ggttcatcgt cggcggcgac ttgccggcgt
     1981 cttacgtgtc ctccacggtg gccggcatcg ccgacggcac gtacacgttc atcggcgaga
     2041 tcctctacac gcacggcgac aagccggacc acaaaccgac caggaacggg caggtctacg
     2101 tcgatccgct cgcgtcggga acggctcacc tcctcgacgg gatcgccggc cacaccacgc
     2161 cgctgctgac gcattgggaa gcgtgggcct gggaccgcga ctggccgcac ttcgaccgac
     2221 tgtacgcgag caggccgcag caacgcttcg tgctgccgag tcttgcctac ggctcgccgg
     2281 aacaggctga cggcattttg tccgcgcacc ccaacgtctg gggaatcatc tcgcgcctcg
     2341 ttgatggacg ctaccgcttc gtcgatccgg ccaaacaggc gagactcggc ccgccgatgt
     2401 tcgacgcgtg cggcgcgctg cggcctgagt ggcgcgcggt gttgaccaag caccgcgacc
     2461 gtctgatgta cgggtcggac gactactcaa ccgcgcgcgt gggatggaac gcgtaccccg
     2521 ggaccatcga caagtaccgc cgcatcgccg gccagttgcc gccggatctc gcccgagaga
     2581 tctcctggga caacgccgcg gcgttgtacg gcgggaggta gtccagcgga cccgcgcacg
     2641 tccgctgtgc ggtcagggtt gcttcaaata cttcccgaga aactcgaggg tccgccgcca
     2701 actatcttca gcggcatccc ggttggcctt ggaggccagt tcccgctcga acgtgtgccc
     2761 ggctccgggg tagaagtaga tctcgtaggg actgtgggca cggtcgagga tggcgcggag
     2821 gcgctccgcg acccggaccg tcgccggtgc gtccgcgctc ccttgcagga tcaggacggg
     2881 agcgctggtg ctggtgaagg ccgcttgcat ctggctcaag gataggtctg tgccgggtcg
     2941 gaacccgccg tagtaggaaa cgcccgcctg gatcttgctg gtcgccgcaa gccaagttgc
     3001 gaagccgccg ccggcggaga agccgacggc accaaccctg ctccccccga ctttctcgtt
     3061 gcgacgaagc acggcaagcg cgtccacgaa atccgcgtag agctcggacg cgtgggcgac
     3121 gatatctgcg tcccgggagg ggccgctgat gcgatacgct tccatgaagg cggggacgag
     3181 acagacatat ccctccctgg cgaggcgctg ggcatagtcc aggtccgccg gctccaagcc
     3241 accagccgtg tgaaggacca gcacgcccgg ataaggtccg tggccgtctg gagtgatcac
     3301 attagcgcgc atgttgtgcc tgacgccgac cagaaccgag gaggatgtgg ccgcgccggg
     3361 ggatacaagc accgccagga gaatcgccgc cacgagggtc caccctacga ggtgccccct
     3421 tgggcctgcc attgctaatt ccccctcttc gctcgcccgc gtgctaaggg caaccgcaga
     3481 tccctggtgc ttccggggac tttcgccccc aaatgcatct cccctctctc acctccccgc
     3541 ccttaggttg tctagcgggc tcgcccgcca gaacctcgcg ctcacatccg ggttcgtgag
     3601 gtgtgcgtat ctaagcgcca tggcgagtga ctcgtgccgg agcacctgcc gcaccaactc
     3661 gaggtctcc
//
LOCUS       DASQGH010000630         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11232, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000630 DASQGH010000000
VERSION     DASQGH010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11232"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1317)
                     /locus_tag="VEW91_12515"
     CDS             complement(121..1317)
                     /locus_tag="VEW91_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92445.1"
                     /translation="MGAILPVLILASIAYSLTVSFINPILVEVARTFHASVGTAGQAR
                     AILSAAGAVAALGATVFADRVPRRWQLLMGLAVLGIGDAGLGLTQSFGPWMALQGVAG
                     IGAAIVGLAGTAAAGDYFDETQRGAAMGWITSGYPIAWLVGMPLIGWIADGWGWRVSY
                     LAAGAGIAALAFLGVLVALPAPRFQTAPPSHYLAGWRVLLATPAARAWVIAELLTTTA
                     WSSFLVYVGAFFGVTYGLTPGKIGAIVAVGALASVAGTLSSGRWGDRWGRRRVLLLST
                     LLAALAIAAPLSLRLTPLASLALLLPYYFISSVRFPTSSTIALSLLPAAPGTMMATRG
                     LTITIGGMTGTILAGLLLEVGGFATVGVAYALLTAAGFAIFWRRIPAAPGEPAMRRED
                     EPNPWR"
     gene            complement(1381..2836)
                     /gene="guaB"
                     /locus_tag="VEW91_12520"
                     /pseudo
     CDS             complement(1381..2836)
                     /gene="guaB"
                     /locus_tag="VEW91_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            2858..>3984
                     /locus_tag="VEW91_12525"
     CDS             2858..>3984
                     /locus_tag="VEW91_12525"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYM92446.1"
                     /translation="MTDPPAIPAAHRLEAAASRRPGTFASIRHRNFRLFFIGQMISLI
                     GTWMQRIAQAWLVLQLTNSPFLLGLVGMLQWLPVLLFSLIGGVIADRVSKRGLIVVTQ
                     TAQMLQAFALGGLVLAGTVQFWHVAVLAVALGLAAAFDMPTRQAFIFEMVEGTDTMNA
                     VALNSTIVNVARLLGPAIAGVAIARVGMAWAFLVNGASFIPVIAALLLMQVRPGQVVH
                     TTTRVLTHLREGIAYLRRTPVALHAIVLLAAESVFAMNFTLLTSVLAKDVLRAGAAGF
                     GFLMSAQGAGALMGAIAVASFSYLGPRPEFLFGGAAVLGLAEVVLGLTRQFPAAAVML
                     GVAGASMVLFTATVNTTLQLNAPDRLRGRVMAMYALVMGGLT"
BASE COUNT          618 a         1399 c         1359 g          608 t
ORIGIN      
        1 aaacgcgggg cgtgacaaag tatcctggac tctacttcgt gggattgcgc tggcttcata
       61 cgcgcaaatc ttcactcatc tacggcgtcg gggaggatgc cgcgcatatt gtagatgcta
      121 ttagcgccag ggattaggtt catcctctcg ccgcatcgcg ggctctccag gcgcagccgg
      181 gatccgccgc cagaagatgg caaagccggc tgcggtgagg agcgcgtacg ccacgcccac
      241 ggtcgcaaag cccccgacct ccaacaggag tcccgccagg atcgtgcccg tcatcccccc
      301 gatcgtgatc gtcagcccgc gggtcgccat catcgtcccc ggcgcggccg gcagcaatga
      361 cagggcgatc gtgctcgagg tcgggaaccg tacgctgctg atgaagtagt atggaagaag
      421 cagcgccaag ctcgcaagcg gcgtgaggcg caggctgagc ggcgcggcaa tcgccagcgc
      481 ggccagcagg gtgctgagca gtagcacccg ccgtctcccc caccggtccc cccaccggcc
      541 cgaggataac gtccccgcca cgctcgcgag cgcgccgacc gcgacgatcg caccgatctt
      601 cccgggcgtc aggccatagg tgacgccgaa gaacgcgccc acgtaaacga gaaaactcga
      661 ccacgccgtg gtggtgagca gctcggcgat gacccaggcc cgagccgccg gggtggccag
      721 cagcacccgc caaccggcca ggtagtggga cggtggcgcg gtctggaagc gcggcgcggg
      781 gagggcaacg agcacgccga gaaacgccag tgcggcgatc ccggcgccgg ccgccaggta
      841 gctgacccgc catccccatc catcggcgat ccacccgatg agcggcatcc cgaccagcca
      901 cgcgataggg tatccgctgg tgatccaccc catcgcggct ccccgctggg tttcatcgaa
      961 gtaatcgccc gccgcggcgg tcccggccaa cccgacgatc gctgcgccga tcccggccac
     1021 accctggagc gccatccacg gcccgaagga ttgggtgagc ccaagcccgg cgtccccgat
     1081 gccgagcacg gccaggccca tgaggagctg ccaccgccgc gggacgcggt cggcgaagac
     1141 cgtggcgccc agagcggcca ccgcgcccgc cgcgctgaga atggcgcgcg cctgcccggc
     1201 ggtgcccacg gaagcgtgga atgtgcgggc gacctcgacg aggatggggt tgatgaatga
     1261 aaccgtaagg gagtaggcga tggacgccag aatcaggacc ggaaggatcg cgcccacccc
     1321 ggatcgggca gggtttggaa gacctgggcg gcgagcagac aagcggagag cagcggacgg
     1381 ctaggacagc gcgtccacgt cgtggggcat gccctcccgc accccggcct ctgtcacccg
     1441 gatgaaccgt ccgttggcgc ggagttcgtc gagggtggcc gcgccgcagt agctcatgcc
     1501 cgagcgcagg ccgccgatca gctgaccgag gaccgcggcg accctgccgc ggtacgggac
     1561 catcgcctcg accccttcgg ggacgatctc ggagaggagg tcgtcctcct gcggcatatc
     1621 ccggcgctcc gcggaggtcc agaggctggc catcccccgg tacaccttgt actgccggcc
     1681 gccccgcgtg accgaggcgc ccgggctctc ctcggttccg gccaggaggt tcccgagcat
     1741 ggccgtctgc gcgccggcgg ccagagcctt caccaggtct ccggaactgc ggatcccgcc
     1801 gtcggcgatg atcggcacac ccgcggccga ggcggcggcg gcacactcca ggatcgcggt
     1861 cagttgcggc acaccggccc cagtcaccac gcgggtggtg cacgtggacc cgggcccgac
     1921 cccgaccttg atcccgtcga cgccgcaggc gatcaggtca cggatccctt ccgccgtggc
     1981 gacattcccg gcgatgagcg gaacctccgg gcagcgggcg cggatcgcct ccacggtttg
     2041 tatggccaga tcggagtggc cgtgggcgat gtcgatgacc agcacatcca cctcttcggc
     2101 caccagggcc gccgcccgct ccaggtaatc ggcccggaca ccgatggccg ccccgacccg
     2161 gagccggccc ttctggtctt tggtggcatt cgggtacttg agccggctct ggatgtcccg
     2221 gctcgtgacc aggcccacca accgcccttc cggatcgacc agggggagtt tctcgatccg
     2281 gtgacggtga aggatttgct tggcctcctc gaccggggtg ccggcgggcg cggtgatgag
     2341 ccgctcgtgc ggggtcatga ccgccgtgat cggacggtcc gggtggtcct cgaacagcac
     2401 atcgcgcgcg gtcacgatcc ccaggagccg gcgctggcgg tcgatgacca agaccccggc
     2461 ggacccgtgc tcctccatga atgccgcggc atgcttgacg gtagcctcgg ggccgagcgt
     2521 gaacggattg tcgatgacga cgctctcggc ccgcttcacc cggcggatct cggccacgtg
     2581 ctcatcgatc ggcaggaatc gatggatgat gccgatgccc ccctcccggg cgatcgcgct
     2641 cgccatccgc gcctcggtca cggtatccat gctcgcgctg acaatcggga tcgcgagcgc
     2701 aatgctccgg cacagccggg tccgggtatc gatctggcga cgggtgctca cccgagaccg
     2761 ccgcggcacc agcagcacat cgtcaaacgt cagtccaagc tcaatcgggc cgttcatacc
     2821 accgccaaga tagcatgtcc gatgctaata tggaagcgtg acggatccac cggcgatccc
     2881 cgccgcgcac cggttggagg cagcggcatc ccggcgcccc ggcacgtttg cctccattcg
     2941 ccaccgcaac ttcaggctgt tcttcatcgg gcagatgatc tcgctcatcg gcacctggat
     3001 gcagcggatc gcgcaggcct ggttggtgct ccagctgacc aactcgccgt ttctcctggg
     3061 gctcgtcggg atgctccagt ggctcccggt cctcctgttt tcccttatcg gcggcgtgat
     3121 cgccgaccgg gtcagcaagc gcggcctcat cgtggtcacc cagacggcgc aaatgctgca
     3181 ggcgttcgcg ctgggggggc tcgtgctggc gggaacggtg caattctggc acgtggccgt
     3241 cctggccgtg gcgctcgggc tggcggcggc gttcgacatg ccgacccggc aggcgttcat
     3301 cttcgagatg gtcgagggga ccgataccat gaacgccgtg gcgctcaact cgaccatcgt
     3361 caacgtcgcc cggctcctgg gcccggcgat cgccggggtg gccatcgccc gggtggggat
     3421 ggcgtgggcg ttcctcgtga acggggcgag ctttattccg gtcatcgcgg ccctgctcct
     3481 gatgcaggtc cggcccggcc aagtggtcca cacgaccacc cgggtgctga cccacctccg
     3541 ggaggggatc gcctacctgc gccgcacccc cgtggcgctg cacgcgatcg tgctgctggc
     3601 ggcggagagc gtgtttgcga tgaacttcac gctgctcacc tcggtcctgg ccaaggatgt
     3661 gctgcgggcc ggggccgcgg ggtttggatt cctcatgtcc gcgcagggcg ccggcgcgct
     3721 catgggcgcg atcgcggtgg cgtcgttcag ctacctcgga ccacgcccgg agttcctctt
     3781 tggcggcgcg gcagtgctcg gcctggcgga ggtggtcctc ggcctgaccc gccagttccc
     3841 cgccgccgcg gtgatgctgg gggtcgccgg cgcgagcatg gtgctgttca ccgcgacggt
     3901 caacacgacc ctccagctca acgctcccga ccgtctccgc gggcgcgtga tggccatgta
     3961 cgcgctggtg atgggggggc tgac
//
LOCUS       DASQGH010000631        13912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53690, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000631 DASQGH010000000
VERSION     DASQGH010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13912
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53690"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="chrA"
                     /locus_tag="VEW91_12530"
     CDS             complement(<1..830)
                     /gene="chrA"
                     /locus_tag="VEW91_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HYM92447.1"
                     /translation="MTDATTPPLRAERRHALLEVAVLFLKLGWIAFGGPAAHIALMRQ
                     EVVGRRKWLSEQQFLDLLGASNLIPGPTSTELAIYLGYARAGPMGLVVAGLLFILPAM
                     LLVLAFAWAYVRYGSTPQVASLLYGIKPVIIAVIVQAIYALLRSAVKHWRLGVVVVLA
                     LALYLRGLNPLIPLFGLALLVMVVENRARLIGRRRYTRGGPLLVLPLPAIPGVAAVAV
                     AGTAGFSLAALFLTFLKIGATLYGSGYVLLAFLRDDFVHRLGWLTDRQILDAIAVGQF
                     T"
     gene            complement(833..1816)
                     /locus_tag="VEW91_12535"
     CDS             complement(833..1816)
                     /locus_tag="VEW91_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HYM92448.1"
                     /translation="MGRRAFIIQAARQGWAAIALAGATGLSWGRAAAAPSPGPGRWLA
                     RAPLPVERQEVGVAPAGGKVYVLGGYANGRVDQPLNQEYDPGTDTWRDRAPMPLGLNH
                     VAVVGLGGKIYACGGFVQQNRGAVPDAYEYDVRLDRWRALAPLPEKRGAAAAVALGGQ
                     LHLVGGRDLVSVGTHEVYDPATNRWTARAPLPKGRDHLGLVVIADRIHAVGGRFNTFD
                     YNTNLHDEYDPSADVWRSRTPMPTARSGVAAAFLGGRLFVFGGERAGGVFSENEAYDP
                     ATDRWVTMAPMRTPRHGTGAAVVGSLIYIPAGGLTNGGSRPSSVNEAFGLP"
     gene            2047..4110
                     /locus_tag="VEW91_12540"
     CDS             2047..4110
                     /locus_tag="VEW91_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYM92449.1"
                     /translation="MDRQRRRVTRRELLRDAALATGGFAFAGLAPEVWAQTPKLGAQL
                     IGKLEGPEIIRDPAKWPRKFNEAPMLAELVKQGKLPPVEQRVPQEPLVLKPVHEIGRY
                     GGTLRRAFTGPGDSENGNRWVSADKVLFWDYTGTKVMPSVAQAWLLPEDGRSVTIYLR
                     KGMKWSDGQPFTANDFVFWYEDVYLNKDIVPTPHPDFMINGKSGALRKVDDATVRFEF
                     PEPNYLFLDILAGSTSMGGGQALWQMEGRTMGAYTPAHYVRQFHPKYIGKDEADRKAK
                     AAGFDNWVAYIKNRWDWRLNPELPVLTPWRTVVPINTPTWVLERNPYFYEVDTAGNQL
                     PYIDRIQLTLGENLEVINLRAIAGQYDLQERHTAMTKIPVFLENRDKVGYDLHLDPAL
                     NGSDATLQTNQAYDADPEIAKWLQTADFRRALSMGIDRDQLNETFWLGIGTPGSIAPS
                     ETMPYSPGPEWRKKWATLDVQQANELLDKIGLSKKDGEGYRLRTDGKGRLRLEIMTAA
                     GTFIPQTQIGEMIKEQWKKIGIQADAKEHERSLFLTRTTSNQQQICMWPNDGTEVLYL
                     FPRHALPVDPVEAYLGHPIAVWYASGGTQGKAPKDPQLLRALELFRSANGKKTQERYK
                     IAQEIWKILVDQAYSIGTVGQSPATMGVRVVSRKMGNIPSREINAQHARTPGTSQPST
                     FYFKA"
     gene            4170..5156
                     /locus_tag="VEW91_12545"
     CDS             4170..5156
                     /locus_tag="VEW91_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92450.1"
                     /translation="MLAYLAHRLILAVLTIWAVSVLSFVIIKLPPGDYVTAYIAQMSM
                     SGGYVSEQEAEALRREYGLDKPVYVQYLRWMKMVVHGRFGVALEWKRPVVEVIGDRLW
                     LTMVVSVGAIVITWVLALPIGIYSAVRQYSVGDYLATFVGFIGLAVPSFMLALVLMYF
                     GFKLFNANIGGLFSDEFALAPWSLAKVWDLTKHLPLPTLILGFAGTAQLIRIMRANLL
                     DELGKPYVVTARARGLSEARVILKYPVRVALNPFASTIGYLLPYVVSGSIIVSLVLGL
                     PTVGPLLLRALIAQDMFLAGTIVLLLGVLTVVGTFLSDLLLLWIDPRIRLEG"
     gene            5244..6323
                     /locus_tag="VEW91_12550"
     CDS             5244..6323
                     /locus_tag="VEW91_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYM92451.1"
                     /translation="MSVATQWQLMWWRFRRHKLAMVGSLVVLLFYGVVPFADFLAYAV
                     PTASEAQRSLLPPQPIHWLDSGRLWPHVYGFTGQRDPVTLKRVYTLDPAKKVPVRFLA
                     RGFEYRLFGLIPADRHLLGVEGAKSEETLFLLGTDEQGRDLWSRLMYATRTSLTIGLL
                     GVTISLVLGVFLGGVSGFYGGVIDTVIQRVIEILRSIPTIPLWMGLAAALPNSWTILQ
                     VYFAITLIISLIGWTELARVVRGRFLSLREEEFIMAAELAGSSRSRIIVSHMVPSFLS
                     HIIAATTLALPAMVISETSLSFLGLGLRPPAVSWGVLLQQAQSVQALAISPWLLFPAV
                     PVIVVILAFNFLGDGLRDAADPYGH"
     gene            6323..7351
                     /locus_tag="VEW91_12555"
     CDS             6323..7351
                     /locus_tag="VEW91_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003572415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYM92452.1"
                     /translation="MDADGQPILEVRELRTYFFPEEGTVKAVDGVSFEVYPGRTLGIV
                     GESGCGKSVTARSILRIVERPGRIVGGEIHLRRGSGLGNGRAATVDLVKLDPTGPEIR
                     HIRGGDIGLVFQEPMTSFSPVHTIGNQMQETIRLHTGGDQAAAHARAIEMLRLVGVPR
                     PERRIGEYAFQLSGGLRQRAMIAVALSCHPRILIADEPTTALDVTTQAQILDLLRSLQ
                     AQERMAIVLITHNLGVVAEMCDEVVVMYLGRVVERGPVDAIFHAPKHPYTRALLRSIP
                     SIKAHVRTKLPTIAGSIPHPYNRPSGCPFHPRCPDFMPGVCDRSEPGLRVVGEGQAAS
                     CFLYEAPR"
     gene            7348..8376
                     /locus_tag="VEW91_12560"
     CDS             7348..8376
                     /locus_tag="VEW91_12560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HYM92453.1"
                     /translation="MTAAARTPGRLLAVTGLRKYFPIRKGFLRKVVGQVRAVDDVSFS
                     LDRGETLALVGESGCGKTTTARCILRALSPTEGQILFRTDEGALVDVAQLPKSRLRPL
                     RRQMQMIFQDPFSSLNPRKTLLDIVGEPLLVNGMRNRQERAERVAELLRLVGLRPEYM
                     RRFPHAFSGGQRQRVGIARALALNPSLVVADEPVSALDVSVQAQILNLMVDLQAQLGL
                     TYLFVAHDLSVVKHVSDRVAVMYVGQIVEQAPTASLFAAPKHPYTEALLSAVPKPDPR
                     LRAQRIVLQGEVADPAHPPSGCYFHPRCPYAAPICRAERPRLEVVAPGHLVSCHRARE
                     LSLKGVET"
     gene            8481..9245
                     /locus_tag="VEW91_12565"
     CDS             8481..9245
                     /locus_tag="VEW91_12565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYM92454.1"
                     /translation="MSSIYRRYAEAYVKAGQGRWSLQLVPWVTSIFDRHGTGPTSLVD
                     VACGSGEFALTMAQRGLQVTGVDQSPEMLAIARRSAQEKRIAITFLEQDMRRLLLPAP
                     VDAATCLYDSLNYLTNDADFRQALSAIAAALRPGGLFLFDMNTIHGLATRWGNRVWLI
                     QDNEDALEVDQSEFDYDAGIATLKVNAFLRREQDLYERVREIHRERGYPVPTIDAALG
                     AAGFEVLGRWSNPEFGEVTSEAGRVFYAARRRDSPG"
     gene            complement(9295..10248)
                     /locus_tag="VEW91_12570"
     CDS             complement(9295..10248)
                     /locus_tag="VEW91_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYM92455.1"
                     /translation="MRRASQVALVPEAAAGPVRERIYVGTCSWAEKTFVKSGFYPPAV
                     RTAEDRLRYYATQFPTVEIDASYFALLPSAYSHRWAEETPPGFVMHAKAFGLFTGHAA
                     SVERLPPGFASLLPPNLREAREVRVAGVPEEFLNACWDQFREFLAPLARAGKLGYVLF
                     QLPPGVKYSPEALEAMRGWKRELSGCRLAVEFRHRDWSRHPEALEFLAREGLIYVMVD
                     FPDLPRLMPVVETVTSEWAVVRFHGRNRAGWSRAGAPTGERYDYNYSVDELRPWADAV
                     ERLSARARRLFAMFNNHARGNMARNAHAFQGLLEGVSPRAP"
     gene            complement(10249..11607)
                     /locus_tag="VEW91_12575"
     CDS             complement(10249..11607)
                     /locus_tag="VEW91_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYM92456.1"
                     /translation="MDRVFQYIDAHRDEYVDLLRQLCRQPSIAAQGLGLGETAEKVEG
                     LMAESGIRARTIPVEGGAPVVYGEVKGKIPQTLLFYNHYDVQPPEPLEEWASNPFGAE
                     IRDGKIYARGVADNKGNLVARLCAVDAMLHAWGEVPATVRFIVEGEEEIGSVHLPRFV
                     QEHRDLVVADACIWEGGYRDLQENVVLSLGVKGICYVELEARGANRDLHSSIATIVPN
                     PAWRLVWALSTFKDRNERVMIPGFYDAVVEPTEDELAQLRRLAPLRDDAAQHKDYGLD
                     RFLLGVSGVELLKRNLFQPTCTICGITSGYGGPGSKTVLPHRAVAKVDFRLVPNQRAE
                     EILLKVKTHLAQHGFADIAVRPLGLEEPSKTPLGAEIVKVVSESARRIYSREPLVYPS
                     MAGTGPMHVLTGERGIPTIGTGVGYAHSNNHAPNENIRIADYVDGIKHIAMILHLFAG
                     GR"
     gene            complement(11641..12378)
                     /locus_tag="VEW91_12580"
     CDS             complement(11641..12378)
                     /locus_tag="VEW91_12580"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HYM92457.1"
                     /translation="MSYVKLKHTLAEIALAAIEHPDRARPAAALLAYARRSGDQGCAP
                     VAALARAVGLAPDEVRRLLVDTGLFESQGEGGAGCIALAAPFRPFAPYLRRQTARTAA
                     AGQLVASPRRPGIPEEIWRAAALFNAGLFFECHEYLEDVWRAAPASERAFYHGLVQAA
                     AGCYHLEKGNAHGARTLIQKAAAKLEPYAPAHRGVAIAAFLAGLRRVLGRVDRVPPAR
                     DRVALPVLDLVGVPRVRRREPLRRSGI"
     gene            complement(12413..>13912)
                     /locus_tag="VEW91_12585"
     CDS             complement(12413..>13912)
                     /locus_tag="VEW91_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYM92458.1"
                     /translation="PHSGAVELLLRHHDRTRAPQTLLIVTRTLEKMGRGGIYDQLGGG
                     FHRYSVDASWIVPHFEKMLYDNSELLRNYVHAYQATGAAFFREIAEGILGYATGTLMD
                     HTHGGFYASQDADQSLEDDGDYFTWMEEEAAAILPVDELEVVRKTYRLEGRGEMHHDP
                     RRHVLFVDKDPDVVGALLERPADEARDVLARAQRRLRDARGLRRAPFVDPTIYAGWNG
                     MMISAFLEAARGLGRKDARDVAFRALDRIVRDAYRSGEADGGRGFRHLASGPGGVSGL
                     LDDQVHMARALLDAFEQAGDPRYLRLAEETMAYVLDEFQAPWGAFFDVARSTAADVQR
                     PAGLGTPYVPVQDSPTPSGNAVAVLVLDRLAVVMGEARYRDAAERALRACAPGNATHG
                     LFAASLFLALDLHLDPPPHVVVVGPRRDPRVQALLQAALSTYRPGAIVQVHDPSEGGP
                     PLPPLLPAPSGGERGPQAFVCSAAACAPPASTPEALSETVKAFGRLPVG"
BASE COUNT         2240 a         4875 c         4668 g         2129 t
ORIGIN      
        1 gtgaactgcc cgacggcgat ggcgtcgagg atctggcgat cggtgagcca gccgagccgg
       61 tgcacgaagt cgtcgcgcag gaacgccaga agcacgtagc cgctgccgta gagtgtggcg
      121 ccgatcttca ggaaggtgag gaacagcgca gccaggctaa agcccgcggt gccggccacg
      181 gcgaccgcgg cgactcccgg gatggccggg agcggaagga ccagtagcgg cccgccgcgc
      241 gtgtaccgtc tacggccgat gagccgggcg cggttctcga cgaccatgac gagcagcgcc
      301 agcccgaaca gcgggatgag cgggttgagc ccgcggagat agagcgcaag ggcgagcaca
      361 actacgacgc cgagccgcca gtgcttcacc gccgagcgca agagcgcgta gatcgcctgc
      421 acgatcacgg cgataatgac cggcttgatg ccgtagagca aagaggcaac ctgcggcgtc
      481 gaaccatagc gcacgtacgc ccaggcgaac gcgaggacga gcagcatggc cggcaggata
      541 aagagcagac cggccacgac caagcccatc ggtcccgccc gcgcatagcc gagatagatg
      601 gcgagctccg tcgaggtggg ccccgggatc agattgcttg cgcccaggag gtcgaggaat
      661 tgctgctcgc tgagccactt gcgtcgcccc acgacctcct gtcgcatcag cgcgatgtgt
      721 gcggccgggc cgccgaaggc gatccatccg agcttcagga agaggaccgc cacctccaaa
      781 agggcgtgcc gccgttccgc gcgcagggga ggggtcgtcg catcggtcat ggtcagggca
      841 gcccgaacgc ctcattcact gacgacggac ggctcccccc gttggtcaga cctcccgcgg
      901 ggatgtagat gaggctccca accaccgccg cccccgtgcc gtgacggggg gtccgcatcg
      961 gggccatcgt cacccatcgg tccgtcgcgg gatcgtacgc ctcattttcg gaaaacaccc
     1021 ctccggcgcg ctcgccgccg aagacaaaca gccggccccc gaggaaggcg gcggcgacac
     1081 cgctgcgcgc cgtcggcatc ggagtccggg accgccagac gtctgcggag ggatcgtatt
     1141 cgtcgtgcag gttcgtattg tagtcgaagg tgttgaaccg ccctccgacc gcatggatcc
     1201 tgtcggcgat gaccaccagc ccgaggtggt cgcggccctt cggcagcggg gcgcgcgcgg
     1261 tccacctgtt cgtcgcagga tcgtacacct cgtgcgttcc cacgctgacc agatcgcggc
     1321 ctccgaccag gtgcagttgg ccgccaagcg caactgcggc tgcggctcca cgcttctccg
     1381 gcaagggcgc cagcgcccgc cagcggtcca gccgcacatc gtactcgtac gcgtccggca
     1441 cagcgccacg attctgctgc acgaaccccc cacaggcata gatcttgccg ccaagtccga
     1501 ctaccgcgac gtgattgagc ccgaggggca ttggggcgcg atcgcgccag gtgtcggtcc
     1561 caggatcgta ttcctggttc aacggctgat cgacccggcc gtttgcgtac ccgccaagca
     1621 cgtagacctt cccgccggct ggggcgaccc cgacctcctg gcgctcaact ggaagcggcg
     1681 cccgcgccag ccaccggccc ggccccgggg acggagccgc ggccgcgcgc ccccaggaca
     1741 gccccgtggc gccggccagc gcgatggcgg cccacccctg cctcgcggcc tggatgatga
     1801 acgctcggcg acccaccggg ccgccgcccc ccatccgctt cggccgacga ggtgtgtgtg
     1861 ccatccgccc cccccgcgtg acgcaacctt aacgacgacg cgcctgtcgg ttcaacaaag
     1921 acaaatcgac gtcctctgct ccccccgaga gcgcactgct gcaggtggga cagggggcaa
     1981 gggaagggag gagcctcgtg tcgaatggca gcagtccacc tactcgcgca ccaaggaggt
     2041 ggccccatgg acaggcagcg aaggcgcgtc acgcgacgag agctcctccg ggacgccgct
     2101 ctggcgaccg gggggtttgc atttgccggc ctggcccccg aggtctgggc tcagacgccg
     2161 aagctcgggg cgcagctcat cggcaagctc gaaggccccg agatcatccg cgaccccgcg
     2221 aagtggccga ggaagttcaa cgaggcgccg atgctggcgg agctggtcaa gcaggggaag
     2281 ctcccgccgg tcgagcagcg cgtgccccag gagcccctcg tcctgaagcc cgtccacgag
     2341 atcggccgct acggcggcac tttgcgccgg gccttcaccg gccccggcga cagcgagaac
     2401 ggcaaccgct gggtctccgc cgacaaggtc ctcttctggg actacacggg gaccaaggtc
     2461 atgccgtcgg tcgcccaggc gtggctgctc cccgaggatg ggaggtcggt cacgatctac
     2521 ctccgcaagg gcatgaagtg gtcggacggc cagccgttca cggccaacga cttcgtcttc
     2581 tggtacgagg acgtctacct caacaaagac attgtgccga cgccgcaccc ggacttcatg
     2641 atcaacggca aatccggagc cctcaggaaa gtcgacgacg cgacggtgcg cttcgagttc
     2701 ccggagccga actacctctt tctggacatc ctggcgggga gcacgtccat gggcggtggg
     2761 caggcgctct ggcagatgga aggccgcacc atgggcgcct acacgccggc ccactacgtc
     2821 aggcagttcc acccgaagta catcgggaag gacgaggcgg accggaaggc caaggccgcg
     2881 ggctttgaca actgggtcgc ctacatcaag aacaggtggg actggcggct caaccccgag
     2941 ctgcccgtgc tgacgccgtg gaggacggtg gtgccgatca acaccccgac ctgggtgctg
     3001 gagcggaacc cttacttcta cgaagtggac acagccggaa atcagctccc gtacatcgac
     3061 cgcatccagc tgaccctcgg cgaaaacctg gaggtgatca acctccgagc gatcgccggc
     3121 cagtacgacc tccaggagcg ccacaccgcc atgacgaaga tcccggtctt cctggagaac
     3181 cgtgacaagg tcggctatga cctccacctc gatccggcgc tcaacgggtc cgatgcgacg
     3241 ctgcagacga accaggcgta cgatgcggat cccgagatcg cgaagtggct ccagaccgcg
     3301 gacttccgcc gtgcgctgtc gatgggcatc gaccgcgacc agctcaacga gacgttctgg
     3361 ctcgggatcg ggacacccgg ctccatcgcg ccgagcgaga ccatgccgta cagcccgggg
     3421 ccggaatggc gcaagaagtg ggccacgctc gatgtccagc aggccaacga gctcctcgac
     3481 aaaatcgggc tctcgaagaa ggacggcgag gggtaccggc tccgcaccga cggcaagggg
     3541 cggctccgcc tcgagatcat gaccgcggcc ggaaccttca ttccccaaac gcagatcggg
     3601 gagatgatca aggagcagtg gaagaagatc gggatccagg ccgacgccaa ggagcacgag
     3661 cggagcctct tcctcacgcg gaccaccagc aaccagcagc agatttgcat gtggccgaac
     3721 gacggcaccg aggtgctcta cctctttccc cgccacgcgt tgccggtgga tccggtcgaa
     3781 gcctacctcg ggcaccccat cgccgtctgg tacgcgtcgg gcggcacgca ggggaaggcg
     3841 ccgaaggacc cgcagcttct ccgggccctc gagctcttca ggtcggcaaa cgggaagaag
     3901 acccaggagc gctacaagat cgcgcaggaa atctggaaga tcctcgttga ccaggcgtac
     3961 tcgatcggga ccgtcggcca gtcgccggcg acgatggggg tccgggtcgt gagccgcaag
     4021 atggggaaca tcccgtcccg ggagatcaat gcccagcacg cccggacgcc ggggacgtct
     4081 cagccgtcga cgttttactt caaggcgtag ggcaggatcg gtgtggtgcc gggaaaggga
     4141 gacgcacgtt gaataggcgc tgagcgctca tgctcgccta cctcgcccac cggctgatcc
     4201 tggccgtcct gaccatctgg gcggtttccg ttctgtcctt tgtcatcatc aagctccccc
     4261 caggggacta cgtgaccgcc tacatcgctc agatgtccat gtcggggggt tacgtctccg
     4321 agcaggaagc cgaggcgctc cggcgggagt acggcctcga caagccggta tacgtccagt
     4381 atctgcggtg gatgaagatg gtcgtccacg gccggttcgg cgtggcgctc gagtggaagc
     4441 gccccgtcgt cgaggtgatc ggagatcggt tgtggttgac gatggtggtc tccgtggggg
     4501 ccatcgtcat cacgtgggtg ctcgcgctgc ccatcgggat ctactcagcc gtgcggcagt
     4561 actcggtcgg cgactacctc gccacgttcg tggggtttat cgggctcgcg gtcccgagct
     4621 tcatgctggc gctcgtcctc atgtacttcg gcttcaagct cttcaacgcg aacatcggcg
     4681 gcctgttctc agacgagttc gccctggcgc cgtggagcct cgccaaggtg tgggacctca
     4741 ccaagcactt gccgctcccc acgctgatcc tgggcttcgc cggtaccgcc cagctcatcc
     4801 ggatcatgcg ggcgaacctt ctggacgagc tcggcaagcc ctatgtcgtc actgcgcggg
     4861 cccgcgggct cagcgaggcg cgcgtgatcc tcaagtaccc ggtccgggtg gccctgaacc
     4921 cattcgccag caccatcggc tacctcttgc cctacgttgt ctcgggcagc attatcgtct
     4981 ccctcgtcct cggcctgcca acggtcggcc ccctcctcct ccgcgccctc atcgctcagg
     5041 acatgttcct ggctggaacg atcgtgctgc tgctgggggt gctgaccgtg gtgggcacct
     5101 tcctctcgga cctcctgctg ctctggatcg acccccgcat tcgtctggag gggtagtcga
     5161 tcgccgaacc ggtcgttccc tctcaccccg ccgtcccccc tgacggggac aggctggcgg
     5221 aggccggcgt cgccgaggag cggatctcgg tcgccaccca gtggcagctc atgtggtggc
     5281 gcttccggcg ccacaagctc gcgatggtcg ggagcctcgt ggtcctgctg ttctatggcg
     5341 tcgtgccctt cgcggacttc ctcgcctacg cggtgccgac ggcgtcggag gcccagcgct
     5401 ccctcctgcc gccccaaccc atccattggc tcgacagcgg acggctttgg ccgcatgtct
     5461 acggcttcac cgggcagcgg gacccggtga cgctcaagcg cgtctacacc cttgatcccg
     5521 cgaagaaggt ccccgtgcgc ttcttggccc gcggcttcga gtaccggctc ttcggcctga
     5581 tcccggccga ccggcacctc cttggggtcg agggggcaaa gtccgaggag acgctgttcc
     5641 tgctcgggac ggacgagcag gggcgcgacc tctggtcgcg gctcatgtac gcgacgcgga
     5701 cgtccctgac gatcggcttg ctgggggtga cgatctccct ggtactcgga gtgttcctgg
     5761 gcggcgtctc gggattctac ggcggcgtca tcgacacggt catccagcgt gtgatcgaga
     5821 tcctccggtc gatcccgacc atccccctct ggatggggct ggcggcggcg ctgccgaaca
     5881 gttggacgat cctccaagtc tacttcgcga tcacgctcat catctcgctg atcggctgga
     5941 ccgaactggc ccgggtcgtg cgcggccgtt tcctctcgct tcgggaggag gagttcatca
     6001 tggccgccga gctcgccggc agcagccggt cccgcatcat cgtgagccac atggtgccat
     6061 cgttcttgag ccacatcatc gccgcgacca cgctggccct cccggccatg gtcatcagcg
     6121 agacctcgct ctcgttcctg gggctcgggc ttcgaccccc cgccgtcagc tggggggtgc
     6181 tgctccagca ggcccaaagc gtccaggccc tcgccatctc gccgtggctc ctcttccccg
     6241 cggtcccggt gatcgtggtc atcctcgcgt tcaacttcct gggcgacgga ttgcgcgacg
     6301 ccgccgatcc ctacgggcac tgatggacgc ggacgggcag cccatcctgg aggtccgcga
     6361 gctccggacc tacttcttcc cggaggaggg cacggtcaaa gcggtcgacg gcgtgagctt
     6421 cgaggtctac cccggccgga cgctcggcat cgtgggcgag agcggctgcg gcaagagcgt
     6481 gacggcaagg tcaatcctgc gcatcgttga acgccccggc cggatcgtgg gcggagaaat
     6541 ccatctccgg cgcgggagcg gcctgggcaa cggccgcgca gccaccgtcg atctcgtgaa
     6601 gctcgacccg accggcccgg agatccggca catccggggc ggcgacatcg gccttgtgtt
     6661 ccaggagccg atgacctcct tcagcccggt ccacaccatc ggtaaccaga tgcaggagac
     6721 gatccggctt cacaccgggg gcgaccaggc tgcggcgcat gcgcgggcga tcgagatgct
     6781 gcgtctcgtc ggcgtcccca ggccggagcg gcggatcggc gagtacgcct tccagctgag
     6841 cgggggactg cgccagcgtg cgatgatcgc ggtggcactc tcgtgccatc cccggatcct
     6901 catcgccgac gagccgacca cggcgctgga tgtgaccacc caggcccaga tcctcgatct
     6961 cctgcggtcc ctccaggcgc aggagcggat ggcgatcgtc ctgatcacgc ataacctcgg
     7021 cgtggtggcc gagatgtgcg acgaggtggt ggtcatgtac ctcgggcgcg tggtggaacg
     7081 cggccccgtg gacgccatct tccacgcgcc aaagcatccc tacacccggg cactgctacg
     7141 ctccatcccg tcgatcaaag ctcacgtgcg cacaaagctc ccaacgatcg ccggctcgat
     7201 cccgcacccg tacaaccggc cgtcgggctg cccattccac ccgcggtgcc ccgatttcat
     7261 gccgggcgtc tgcgaccgga gcgagccggg ccttcgtgtg gtgggcgagg ggcaggcggc
     7321 aagttgcttc ctctacgagg cgccccgatg acggccgccg cccggacgcc gggccgcctc
     7381 ctcgcggtca ccggcctgcg gaagtacttc ccgatccgca agggtttcct gcgaaaggtc
     7441 gttggccagg tgcgggctgt ggatgatgtg agcttctccc tcgaccgggg cgaaacgctc
     7501 gcgctcgtcg gcgagagcgg gtgcggcaag acgaccacgg cccgctgcat cctcagggcg
     7561 ctctccccaa ccgaagggca gatccttttc cgcaccgacg agggcgcgct ggtggacgtc
     7621 gcccagctgc cgaagagccg gctccggccg ctccggcggc agatgcagat gatcttccag
     7681 gacccgttct cttcgctgaa cccccggaaa acgctcctcg acatcgtggg cgagccgctc
     7741 ttggtcaatg gaatgcgaaa ccgccaggag cgggcagagc gcgtcgcgga attgctccgg
     7801 ttggttggcc tccggcccga atacatgcgc cgcttccccc atgccttcag cggcgggcag
     7861 cgccagcgcg tcgggatcgc ccgcgccctc gccctcaacc ccagcctggt cgtcgccgat
     7921 gagccggtct ccgccctcga cgtgtcggtg caggcccaga tcctcaactt gatggtggac
     7981 ctccaggcgc agctcgggtt gacctacctc ttcgtcgcgc acgacctctc cgtggtcaag
     8041 cacgtgagcg accgggtcgc cgtgatgtac gtgggccaga tcgtcgagca ggcgccgacc
     8101 gcgtccctct tcgccgcgcc caagcacccg tacaccgagg cgctgctctc cgcggtcccg
     8161 aagcccgacc cgcggctccg cgcccagcgg atcgtcctcc agggcgaggt ggccgacccc
     8221 gcccacccgc cgtcgggctg ctacttccat ccgcggtgcc cgtacgccgc gcccatctgc
     8281 cgggccgagc gcccccggct cgaggtagtc gcccccggcc acctggtcag ctgccaccgg
     8341 gcgcgggagc tctccctgaa gggcgtggaa acctgagcag tggaggaagc gacaatccag
     8401 gccccgatgc acggacgccg cttgaggacc aggaagcagc tggacggcgg gagaaccctc
     8461 tcctcaccta gtccatggcg atgagcagca tctaccggcg ctacgccgag gcgtacgtca
     8521 aggcgggcca gggccgctgg agcctccagc tggtcccctg ggtgacgagc atcttcgatc
     8581 gccatgggac cggcccgacc tcgctcgttg atgtggcgtg cggatcgggg gagttcgcgc
     8641 tcaccatggc ccaacggggg ctccaggtca cgggggtgga ccagtccccc gagatgctgg
     8701 cgatcgcgcg acgcagcgcg caggagaaga ggatcgccat cacgttcctc gagcaggaca
     8761 tgcgccgcct cttgcttccc gcgcccgtcg atgcggccac ctgtctgtac gacagcctca
     8821 actacctcac caacgatgcc gacttccggc aggccctgag cgcgatcgcc gccgccctcc
     8881 gccccggggg cctgttcctc ttcgacatga acacgatcca cgggctcgcg acgcggtggg
     8941 ggaaccgggt ctggctgatc caggacaacg aagacgcgct cgaagtcgac cagagcgagt
     9001 tcgattacga tgccgggatc gcgaccctca aggtcaacgc cttcctgcgc cgggaacagg
     9061 acttgtacga gcgggtccgc gagatccacc gagagcgcgg gtacccggtg cccacgatcg
     9121 acgcggcgct cggggccgcc gggttcgagg ttctgggacg gtggagcaac ccggagtttg
     9181 gcgaggtgac ctcggaggcc gggcgggtgt tctacgccgc ccggcggcgc gactcgcccg
     9241 ggtaggtcga gcggcgtttg ttcgcggtct ccatgcgcgc ggcgaccggc ccggtcaagg
     9301 agcccgcggc gagacgccct cgagcagacc ctgaaacgcg tgggcgttcc tggccatgtt
     9361 gccccgggcg tggttgttga acatggcaaa gagccggcgg gcgcgcgcgg agagccgctc
     9421 gaccgcgtcg gcccacggcc gcagttcgtc aaccgagtag ttgtagtcgt agcgctcccc
     9481 ggtgggcgca cccgcgcggc tccacccggc gcggttccgg ccgtggaacc ggaccaccgc
     9541 ccactcggac gtcaccgttt ccacgaccgg catcagccgc gggaggtccg ggaagtccac
     9601 catcacgtaa atcagccctt cccgcgccag gaactccagc gcttcgggat ggcgtgacca
     9661 gtcccggtgg cgaaactcga ccgcgaggcg gcagccgctc aactcccgct tccatccccg
     9721 catcgcctcc aacgcctcgg gggagtactt gacccccggg gggagctgaa acagcacgta
     9781 gccaagcttg ccggcgcggg cgagcggggc gaggaactcg cggaactgat cccaacaggc
     9841 gttgagaaac tcctccggca cgccggccac ccgcacctcg cgggcctccc gaaggttcgg
     9901 gggaaggagc gacgcgaagc cgggcgggag gcgctcgacc gaggcggcgt ggccggtaaa
     9961 caagccgaac gccttggcgt gcatgacgaa cccgggtggg gtctcctccg cccatcggtg
    10021 gctgtaggcg gacggcagca gcgcaaagta ggaggcatcg atctccaccg tgggaaactg
    10081 tgtcgcgtaa taccggagcc ggtcctccgc ggtgcggacc gctggcgggt agaacccgct
    10141 cttgacaaac gtcttctccg cccacgaaca ggtcccgacg tagatccgct cccgcaccgg
    10201 tcccgcggcc gcctcgggca ccagcgcgac ctgggaggcg cgccgcatct accggccgcc
    10261 tgcgaacagg tgcaggatca tcgcgatgtg cttgatcccg tcgacatagt ccgcgatccg
    10321 gatgttctcg ttcggcgcgt ggttgttgct gtgggcgtag cccacaccgg tcccgatcgt
    10381 ggggatgcct cgttcccccg tgagcacgtg catcgggccg gtgccggcca tcgacgggta
    10441 gacgagcggt tcccggctgt agatccgccg cgcgctctcc gagacgacct tcacgatctc
    10501 ggccccgagc ggggttttgg acggctcctc gaggccgagc ggtcgcacgg caatgtccgc
    10561 gaatccatgc tgggccaggt gggtcttcac cttcaggagg atctcttccg cgcgctggtt
    10621 gggcaccagc cggaagtcca ccttcgccac ggcccggtgg gggaggaccg tcttgctccc
    10681 ggggcctccg tacccggagg tgatcccgca gatcgtgcag gtcggctgaa agaggtttcg
    10741 cttgaggagc tcgaccccgg acaccccgag gagaaaccgg tccagcccgt agtccttgtg
    10801 ctgcgccgca tcgtcgcgga ggggcgcgag gcggcgcagc tgggccagct cgtcctcggt
    10861 gggctcgacc acggcgtcgt agaatcccgg gatcatgacg cgctcgttcc ggtccttgaa
    10921 cgtcgaaagc gcccagacaa gccgccacgc agggttcggc acgatggtgg cgatcgacga
    10981 gtggaggtcc cggttggcgc cgcgcgcctc cagctcgacg tagcagatcc ctttcacccc
    11041 gagcgagagc acgacgttct cctgaaggtc gcggtatccg ccctcccaga tgcacgcgtc
    11101 ggcgaccacg aggtcacggt gctcctggac aaaccgcggc aggtgcacgc tcccgatctc
    11161 ctcctcccct tccacgataa accgcacggt cgccggcacc tcgccccacg catgcagcat
    11221 cgcgtcgacg gcgcagaggc gcgccaccag gttccccttg ttgtccgcaa cgccgcgcgc
    11281 gtagatcttg ccgtcgcgga tctcggcgcc aaacgggttg ctggcccact cctcgagcgg
    11341 ctccgggggc tggacatcgt agtggttgta gaacaggagg gtctgcggaa tcttgccctt
    11401 cacttctccg tagacgacgg gcgcgccgcc ttcgacgggg atggtgcgcg cgcggatgcc
    11461 cgattccgcc atgagcccct cgaccttttc cgcagtctcg ccgaggccaa ggccctgcgc
    11521 ggcgatgctg ggctgtcggc acaactggcg caacagatct acgtactcgt cccgatgcgc
    11581 gtcgatgtac tgaaatacgc ggtccataca cgctcctcgt gaccgacggg ggattatggt
    11641 ctagattcca gatcggcgaa ggggttccct gcgccgcacg cgcgggaccc ccacgaggtc
    11701 caacaccggg agcgccaccc gatcgcgcgc ggggggaacc cggtccaccc gtcccaggac
    11761 ccgccggagc ccggcgagga acgcggcgat cgcgaccccc cggtgcgccg gggcgtacgg
    11821 ctcgagcttg gcggcggcct tctggatgag ggtccgcgcg ccgtgcgcgt tgcccttctc
    11881 gaggtggtag cagccggcgg cggcctgcac gagcccgtgg tagaacgccc gctcggaggc
    11941 gggagcggcg cgccagacgt cctcaaggta ctcgtggcac tcgaagaaca atccggcgtt
    12001 gaacagtgcc gcggcgcgcc agatctcctc cgggatcccc ggacggcgcg gcgaggcgac
    12061 caactgtccg gccgcggccg tccgggcggt ctgccgccgc agataggggg caaaggggcg
    12121 aaagggcgcc gccagggcga tgcagccggc gcctccttca ccctggcttt cgaacagccc
    12181 cgtgtccacc agcagccgcc gcacctcgtc gggcgccagc ccgaccgcac gggcgagcgc
    12241 ggccacgggg gcgcagccct ggtctccgct tctccgcgcg tacgcaagga gcgcggccgc
    12301 cgggcgcgcg cggtccgggt gttcgatcgc ggcaagcgcg atctcggcca gcgtgtgctt
    12361 caacttaacg taactcacga acgctcagcc cgggggccgg gcgcgagccc catcacccca
    12421 cggggagccg gccaaacgcc ttgacggtct cgctcagcgc ctccggagtc gacgcgggag
    12481 gcgcgcaggc ggcggcgctg cacacgaagg cctggggccc gcgttctccg ccggaggggg
    12541 cggggagcag gggaggcagt ggggggcctc cctctgaggg atcgtgtacc tggacgatcg
    12601 caccggggcg atacgtgctc agcgcggcct ggaggagtgc ctggacgcgt ggatccctgc
    12661 gcgggccaac gacgacgacg tgcggcggcg ggtcgaggtg cagatcgagc gccaggaaca
    12721 ggctggcggc gaacaggccg tgcgtcgcgt tgccgggggc gcacgcccga agcgcccgct
    12781 cggccgcgtc gcgatagcgc gcctcgccca tgaccaccgc gaggcggtcg aggaccagga
    12841 ccgcgaccgc attgcccgac ggcgtgggcg agtcctgcac cgggacatac ggcgtgccca
    12901 gcccggccgg ccgctggacg tcggccgccg tggaccgcgc cacgtcgaag aatgctcccc
    12961 acggcgcctg aaactcatcg aggacgtacg ccatcgtctc ctcggcgagg cggaggtagc
    13021 gcggatcacc cgcctgctcg aacgcgtcga ggagtgcgcg cgccatgtgc acctgatcgt
    13081 cgagcagccc ggacaccccg ccgggtccgc tggcgagatg gcggaacccc ctcccgccat
    13141 ccgcctcccc ggagcggtag gcatcgcgca cgatccggtc gagcgctcga aacgccacgt
    13201 cccgggcatc cttccggccg agcccgcgcg ccgcctcgag aaacgccgag atcatcatcc
    13261 cgttccaacc ggcgtagatg gtcggatcga cgaacggggc gcgccggagc ccccgggcat
    13321 cccgcagccg ccgctgggcc cgcgccagca cgtcccgcgc ctcatcggcg ggccgctcga
    13381 ggagcgcccc caccacgtcg gggtctttgt cgacgaacag gacgtgccgg cgggggtcgt
    13441 ggtgcatctc ccctcgcccc tcgaggcggt acgtcttccg gactacctcc agttcatcga
    13501 ccgggaggat ggccgcggcc tcctcctcca tccaggtgaa gtagtccccg tcgtcttcga
    13561 ggctctggtc cgcgtcctgg ctcgcgtaga atccgccgtg ggtgtggtcc atgagcgtcc
    13621 cggtcgcgta gcccaggatg ccctcggcga tctccctgaa gaatgcggcc ccggtggcct
    13681 gataggcgtg gacgtagttg cgtaggagct cggagttgtc gtacagcatc ttctcgaagt
    13741 gcgggacgat ccacgatgcg tcgaccgagt accggtgaaa cccgccgccc aactggtcat
    13801 agatcccgcc ccgccccatc ttctccagcg tgcgcgtgac gatcaggagg gtctgcggag
    13861 cccgggtccg gtcgtggtgc cgcagcagga gttcgacggc gccggagtgc gg
//
LOCUS       DASQGH010000632         4572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53707, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000632 DASQGH010000000
VERSION     DASQGH010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53707"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VEW91_12590"
     CDS             <1..1007
                     /locus_tag="VEW91_12590"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYM92459.1"
                     /translation="VARLTGIPPASWQGFRPLAASGGWPTRRRMMLRFAATRDWGGLV
                     RHLADYYAEHGVGLFARYRAFRWIHEADGTGHLEGVAHPDPVRLDDLVGYEVERQPVV
                     DNTRQFLANLPANNVLVYGDRGTGKSSTVKALLTEFHARGLRLIEVPKEHLGDYLQIV
                     THLRGRRERFILFVDDLSFEEHETQYKALKAALEGSLEVRPENVVLYATSNRRHLVRE
                     RFSDRTGPVPDDDDVHIEETVQEKLSFADRFGIHAPFLIPDQDRYIGIATALAARFGI
                     RLPAREVRRRALLWSQWHNGPSCRTARQFVEALRGELALTQADGQGGTAARGRRPRPR
                     "
     gene            complement(1004..1429)
                     /locus_tag="VEW91_12595"
     CDS             complement(1004..1429)
                     /locus_tag="VEW91_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYM92460.1"
                     /translation="MPALEVPGIIRIHHVGIVVRDVDAAAETYQRALGLDAVALEEFR
                     GTARIVFLRVGETLLELIQPLTDNTPWAVALRDRGEGAHHFALEVVDLRQAMRALAAG
                     GTGFLDAHPSRAPGNTLSIFLDPASTGGTLIELVQQIQT"
     gene            1641..2615
                     /locus_tag="VEW91_12600"
     CDS             1641..2615
                     /locus_tag="VEW91_12600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HYM92461.1"
                     /translation="MRVYLTQPIAEQALARLRAAAEVEMNPDPLHMVTREELLAGVRR
                     ADVLFCLLHDRVDAGVLEANANLRLVASMAITPANIDVAAATARGIPVTVIPPLVTEA
                     TADLAFALILATARRVVAADGLLRSGVFPGAQSAYLVGGAVHGQVLGLVGCGAIGAAV
                     ARRAGGFGMRILYTKRHPLSPAEERTLGVTFAPLEALLEQSDIVSIHAAHTPQTHHLI
                     GERELRRMKPGAYLINTSRGPLVDEAALVRALREGWIAGAGLDVYEQEPQVSPGLLGL
                     RNVVLTPHIGSAERETRETIAGVVVDNILAFARGDRPPNVVNPEAYRR"
     gene            2612..3850
                     /locus_tag="VEW91_12605"
     CDS             2612..3850
                     /locus_tag="VEW91_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYM92462.1"
                     /translation="MSGTGGAEGWARGTAAVDYEARVDMAALRAGRLVRARRALADAN
                     LDAVLVWKDENVRYLTSLRPQLIAGKSGLLNGALLPREGGPILFVSGGDRDRAQATMP
                     WVEEFHAIPILEEPGLVHHFASAIVPGVLARHGLPRARIGLDLTSKLLGDQLAASCPG
                     VRWVDGDAAMQAARRIKTGEELAVLEEATALAEAVTATALAHIRPGVRECEVAGEALR
                     TLLRLGGEYAHVTTPFVASGERLAPPTRLATDKLIRDGDLVFVDIGAMWNGYFGDIGR
                     TVVCGTPSAEQRRVYRAVWEGLQAGIAAMRPGATNMEVARAIHDRAAAHGLREHFLSL
                     FIGHGIGCGSNEPPYIGEIFPGAASVTLEAGMVFALEPLIWLPGVRGGGGVRLEDMVA
                     VTETGARCLSRSPYAAELLA"
     gene            3896..>4572
                     /locus_tag="VEW91_12610"
     CDS             3896..>4572
                     /locus_tag="VEW91_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HYM92463.1"
                     /translation="MAQIRGTGKESGRARGGGEGKRGERTRAGGHPFLAGRHEYQPRR
                     VYSTSGTDWQGRVDFDRMRKERLGRAREMMEKHDLGGLILFVGENVRYTTGVYQGNWK
                     NNIFIRYAALPRGGEPILFETVGSDMVCAEIDAPWLTEDVRPAITWKWSEGAEPEMAA
                     RMARSLGEVFREAGVQKERIGIDVMDMVAYEALTKEGLHIVNGWPAMSQARVVKTVDE
                     LECHKIAA"
BASE COUNT          659 a         1532 c         1732 g          649 t
ORIGIN      
        1 gggtcgcgcg cctcaccggg attccgcccg cctcctggca ggggttccgg ccactcgcag
       61 cctccggggg atggcccacg cggcgccgga tgatgctgcg cttcgccgcg acccgcgact
      121 ggggtgggct cgtgcgccat ctcgccgact attatgccga gcacggcgtc gggctgtttg
      181 cccggtaccg ggcgtttcgg tggatccacg aggccgacgg caccgggcac ctcgaaggcg
      241 tcgcgcaccc ggatccggtc cggctcgacg acctcgtggg gtacgaggtc gagcgccagc
      301 cggtggtgga caacacgagg cagttcctcg cgaaccttcc tgccaacaac gtcctggtct
      361 acggggaccg gggcacgggg aagtcctcga cggtcaaggc cctgctcacc gagttccacg
      421 cgcgggggct ccgcctgatc gaggtgccca aggagcacct gggcgactac ctgcagatcg
      481 tgacgcacct caggggccgg cgcgagcgat tcatcctgtt cgtggacgac ctgtccttcg
      541 aggagcacga aacgcagtac aaggccctga aggcggcgct cgaggggagc ctggaggtcc
      601 gccccgagaa cgtcgtgctg tacgccacca gcaaccgccg gcacctcgtc cgcgagcggt
      661 tcagcgatcg gacggggccc gtcccggacg atgacgatgt ccacatcgaa gaaaccgtgc
      721 aggagaagct ctcgttcgcc gaccggttcg ggatccatgc cccgtttctg atccccgacc
      781 aggatcggta catcgggatc gccacggcgc tcgccgcccg gttcgggatc cggctgccgg
      841 cgcgggaggt gcgccgcagg gcgctcctgt ggtcccagtg gcacaacggg ccttcctgcc
      901 gcaccgcccg ccagtttgtg gaggccctcc gcggcgagtt ggcgttgacg caggcggatg
      961 gccagggcgg caccgcagcc cgcggaagac ggcctcgccc gcgttaggtc tggatctgct
     1021 gcaccagttc gatcagggtt cccccggtcg atgcagggtc gaggaagatg ctgagcgtgt
     1081 tccccggtgc tcgagacgga tgggcgtcga ggaaccccgt gcccccggcg gcgagggcgc
     1141 gcatcgcctg gcgcaggtcc acgacctcga gcgcgaagtg gtgggcgccc tcgccccggt
     1201 cgcgcagggc aaccgcccac ggggtgttgt cggtcagggg ctgaatcagc tccaggagcg
     1261 tttcccccac gcggaggaag acgatgcggg ccgtgccccg gaattcctcg agcgctaccg
     1321 cgtcgaggcc gagcgcccgc tggtaggtct ccgcggccgc gtccacatcg cgcaccacga
     1381 tgcccacgtg gtggatccgg atgattccag ggacctcgag cgccggcatc ccgggtgctc
     1441 cctgtgcggt gtggggcagc gtgcacacgt gcccggtcct gattgtacca gagggctccc
     1501 atccccacgt gtcccgggcg cgaggaggga ggattcctcg cgcgggtcta cgaagtccgg
     1561 cgccagcatg ctgagtgcat ctggtccccg aggtgcgccc ctgctcgagc tgccgtgagc
     1621 ggggcgcggg agtgaccagg gtgcgcgtct atctcaccca gccgatcgcc gagcaggccc
     1681 tcgcccgcct gcgggccgcc gcagaggtgg agatgaaccc cgaccccctc cacatggtca
     1741 cgcgggagga gctgctggcc ggcgtccggc gcgcggacgt gctgttctgc ctcctgcacg
     1801 accgggtgga tgccggtgtg ctcgaggcca acgcgaacct ccggctggtc gcgtcgatgg
     1861 cgatcacccc cgccaacatc gacgtcgccg ccgccaccgc ccgcgggatt cctgtgaccg
     1921 tgatcccgcc cctggtgacc gaggcgaccg cggacctcgc gttcgccctg atcctggcga
     1981 cggcgcgacg ggtcgtcgcg gcggacgggc tcctccgctc gggcgtgttc ccgggcgcgc
     2041 aatcggcgta cctggtgggc ggggccgtgc acgggcaggt gctcggcctc gtggggtgcg
     2101 gcgcgatcgg ggcggcggtg gcccggcgcg ccggagggtt cgggatgcgc atcctgtaca
     2161 cgaagcgcca ccccctgtcg cccgccgagg agcgcacgct gggggtgacg tttgccccgc
     2221 tcgaggcgct gctcgagcag tccgacatcg tgtcgatcca cgccgcgcat acgccccaga
     2281 cccatcacct gatcggcgag cgcgaactcc ggcggatgaa gcccggggcc tatctgatca
     2341 atacctcccg cggccccctc gtcgacgagg ccgcgctggt ccgcgcgctc cgggaggggt
     2401 ggatcgccgg cgcggggctg gacgtctacg agcaggagcc tcaggtctcc ccgggcctgc
     2461 tgggactccg caacgtcgtc ctcaccccgc acatcggcag cgcggagcgc gagacgcggg
     2521 agacgatcgc gggcgtcgtc gtggacaaca tcctggcgtt cgcgcggggc gaccggccgc
     2581 cgaacgtggt gaacccggag gcgtaccggc gatgagcggc accgggggag ccgagggatg
     2641 ggcgcggggc accgccgccg tggattacga agcgcgggtc gacatggccg ccctgcgcgc
     2701 gggccggctc gtccgggcgc ggcgcgcgct ggccgacgcg aatctcgacg ccgtgctggt
     2761 ctggaaggac gagaacgtcc gatacctgac cagccttcgc ccgcagctga tcgcggggaa
     2821 gagcggcctg ctgaacggcg cgctcctgcc ccgggagggg ggaccgatcc tgttcgtctc
     2881 aggcggggac cgggaccggg cccaggccac gatgccctgg gtggaagagt tccacgcgat
     2941 ccccattctc gaggaaccgg ggctggtcca ccacttcgcc tcggcgatcg tccccggggt
     3001 cctggcccgc cacggcctcc cgcgggcgag gatcgggctc gacctcacca gcaagctgct
     3061 gggagaccag ctcgcggcgt cctgccccgg cgtgcggtgg gtcgatgggg atgcggcgat
     3121 gcaggccgcg cgccgcatca agacggggga ggagctcgcc gtgctcgaag aggcgaccgc
     3181 actggccgag gcggtcaccg cgaccgcgct ggcgcacatc cggcccggcg tccgggagtg
     3241 cgaggtcgcc ggggaggcgc tgcggacgct cctccgcctg gggggcgaat acgcgcacgt
     3301 gaccacgccg tttgtggcca gcggcgagcg cctggcgccg ccgacccggc tggcgacgga
     3361 caagctgatc cgggacggcg acctcgtttt tgtcgacatc ggggcgatgt ggaacgggta
     3421 cttcggcgat atcggccgga cggtggtgtg cggcacgcct tcggcggagc agcggcgcgt
     3481 gtaccgggcg gtgtgggagg ggttgcaggc cgggatcgcc gcgatgcgcc cgggcgcgac
     3541 caacatggag gtggcccggg ccatccacga ccgggcggcg gcccacggcc tccgggagca
     3601 tttcctgagc ctgttcattg ggcacgggat cgggtgcggg tcgaacgagc ccccgtatat
     3661 cggggagatc ttccccgggg cggcttcggt cacgctcgaa gccgggatgg tgtttgccct
     3721 cgagccgctg atctggctcc cgggggttcg cggcggcggc ggggtccggc tggaggacat
     3781 ggtggcggtc acggagaccg gcgcccgatg cctgtcccga agcccgtacg cagccgagct
     3841 gctggcgtag gtggaaacgg atcaaccccc aagctggggg agtgggaggc ggagcatggc
     3901 gcagattcga gggacgggga aagagtcggg gcgagcgcgg ggggggggtg agggaaagcg
     3961 gggggagcgc acccgggcgg gtgggcatcc gttcctcgcc ggccggcacg agtatcagcc
     4021 ccggcgggtg tacagcacgt ccggcaccga ctggcagggc cgcgtcgact tcgaccggat
     4081 gcggaaggag cgcctgggcc gcgcccggga gatgatggag aagcacgatc tgggcgggct
     4141 catcctcttt gtcggggaaa acgtccggta cacaacgggc gtctaccagg ggaactggaa
     4201 gaacaatatc ttcatccgct acgcggcgct cccccgcggg ggggagccga tcctgttcga
     4261 gaccgtgggc agcgacatgg tctgcgccga gatcgacgcg ccgtggctca cggaggatgt
     4321 ccgcccggcg atcacctgga agtggtcgga gggcgcggag cccgagatgg ccgcgcgcat
     4381 ggcccgctcc ctcggggagg tgttccgcga ggcaggggtg cagaaggaac ggatcggcat
     4441 cgacgtgatg gacatggtgg cctacgaggc gttgaccaag gaagggctgc atatcgttaa
     4501 cggctggccg gcgatgtccc aggcgcgcgt cgtcaagacg gtggacgaac tggagtgcca
     4561 caagatcgcc gc
//
LOCUS       DASQGH010000633         6753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22583, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000633 DASQGH010000000
VERSION     DASQGH010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22583"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1555
                     /gene="rlmD"
                     /locus_tag="VEW91_12615"
     CDS             143..1555
                     /gene="rlmD"
                     /locus_tag="VEW91_12615"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HYM92464.1"
                     /translation="MEPAATRPRPSFRRGDEVALAIDRMAYGGRGVGRIDGYVVFVPD
                     TAPGDRVRARLWRVRPAYAEADLVGVESPSALRTVPPCPHFGPCGGCVWQHLGYADQL
                     RAKESIVRESLAHLAGLRDVEVRPILAPADPWAYRNKMEFTFHPDGTLGLHRRGAFDQ
                     IAPINTCLIQSAAANEALRVVRDWADASGLSRYDARAHTGLLRQVVIREGKRTGEMMV
                     ALITTAPEVPAARDLAARLLAGGRITSVLLGVNPGPSDGLPLAGVSVLAGRPYIHEHL
                     AGLRFRIGLETFFQTNTAQAEQMIEVVDALAGLRGGETAFDLYSGVGTFSLALARRAE
                     RVYGLEIVASAVETARENAALNGIGNAEFISGDVRRGLPDLLARVGPPHLLVLDPPRS
                     GAGSRVMRRVIAAAAPRIIYISCNPTTLAPDLGDLVRAGYVIRTVQPLDLFPHTYHVE
                     CVVLLERNGAPNAEGAAAQA"
     gene            1552..2475
                     /locus_tag="VEW91_12620"
     CDS             1552..2475
                     /locus_tag="VEW91_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92465.1"
                     /translation="MTRQEAFLPGWVRSLVRGAVVGILVGVSVGAVVSAFVVSAYAIL
                     LFYYAAPSAALLGALQGLWLYLERDRWASRREVLLFGAVSGAALGLLGFAPIFVQTNV
                     LLHWRDIVLLLCAAIAGGGAAGRVNASFFVSYARPQVSPGLRRRLVAGGLIVLSIGLF
                     EYFQYGPAVARKLPVPALSERAVRNLPSGHARGARWVGCYEYNGVFFEGSGVVGTAGG
                     AMVFVQKGGALQIDDIGHGRTLRGGIDADGRFWAGTQGEERDGSLLRLLEKGRFLDRD
                     RFKFSFRVSLVKNGSVANTTREDGAGHRVSC"
     gene            complement(2472..3758)
                     /locus_tag="VEW91_12625"
     CDS             complement(2472..3758)
                     /locus_tag="VEW91_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYM92466.1"
                     /translation="MRRILYVILDGLGDRPVPALGGRTPLGAARIPELTSLARRGRTG
                     LVQTVGQGIAPESDVAVTAILGYDPFTYHTGRGVFEAVGAGMAFRDGDLALRGNFATA
                     GDGLRILDRRAGRDLTAEEAGALADAVTWHLRLEAAPADLEVRASIGHRCGVVIRRQG
                     GRLSAKISNTDPAYGRVEGLGVAREQAGDVAEECTPLDPSEEARTAAALVNEFTRRSH
                     DLLDRHPVNARRRARGRPPANLILVRDAGDHLPSMPPIAERFGVQFGCFVEMPVERGI
                     AQLTGMRVIPVPPSGADKAGVYAAWARTAAEEITRSGGLYIHLKGPDEPGHDGDCEAK
                     RAVIEVIDRAFFATLLPGINLEEVVLAVTADHATPCALHRHSDDPVPLLLAGAGVPSD
                     GSREWSEAACAHGSLGMLRGVDLMPLLVRTAGGEPT"
     gene            complement(3755..4630)
                     /gene="dapF"
                     /locus_tag="VEW91_12630"
     CDS             complement(3755..4630)
                     /gene="dapF"
                     /locus_tag="VEW91_12630"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYM92467.1"
                     /translation="MGRLHRNEYVKSHALGNDYLVVDPRSLSFRLSPPVVRALCHRNL
                     GVGSDGILTIERSRRAGFGIRIFNPDGSEAEKSGNGVRIFAKCLWDHGYTRRRIFDIE
                     TRGGVVSVILQLRGRQVRSITVGMGRATFRSRAIPVTGPDREVVDEEVAVGPQRFRVT
                     AVSVGNPHCVVFVDDLASVDLAHAGASLEHHPMFPNRTNVQFARVDSTRSVAIRIWER
                     GAGETMSSGSSSSAVAAACVRHGFTGRDVAVHTPGGILRVHVGGDYDLRLTGPVEETC
                     RGTFSPDLLARLRRG"
     gene            complement(4637..5041)
                     /locus_tag="VEW91_12635"
     CDS             complement(4637..5041)
                     /locus_tag="VEW91_12635"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92468.1"
                     /translation="MRHPRGSEDGRPDAAAADRDRAQAGVLLLSGQYEPGASEEIVPA
                     RIAPALARKAQQLAVRAHRALGCEGLSRVDMFARKGGIVLLEVNTIPGLTPGSLLPKA
                     AAAAGLDFPELVNRIIQGALRRQRQRRRGRGA"
     gene            complement(4948..5760)
                     /locus_tag="VEW91_12640"
                     /pseudo
     CDS             complement(4948..5760)
                     /locus_tag="VEW91_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
     gene            5823..6593
                     /locus_tag="VEW91_12645"
     CDS             5823..6593
                     /locus_tag="VEW91_12645"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYM92469.1"
                     /translation="MSILGVIFDLGSTLIHRTGLELEKEKCGTLAGVAAAEWGYGDPE
                     ALAARLLEIRLDAWRRSDQELVESVATESIAQAFSEAGLPTDAATLQRAEAVFFEPEV
                     RASHLYPGAADTLETLHAMGLRLGMISNATSHNLILDITAKLEIEEYFDPLVSSAGFG
                     WIKPHPAIFTHVLDAWGITAASVVMVGDTLRADIHGAHTVGMRSILVDIEPNPANARF
                     DGPATPMARVTALREIPGLIRRWGTPRVPRKRPRAKRG"
     gene            complement(6580..>6753)
                     /locus_tag="VEW91_12650"
     CDS             complement(6580..>6753)
                     /locus_tag="VEW91_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYM92470.1"
                     /translation="LGGVSTLVAHPASTTSVNMPREVRLAAGIADGLIRVSVGVEDLQ
                     DLLEDFDQALSRV"
BASE COUNT          958 a         2418 c         2358 g         1019 t
ORIGIN      
        1 gcccggggag gctggcgctg gaccatcgga tccataccgg gttctgaccc cgcatgaccc
       61 cgacgcgccc ggcccgattg gccgggggcc gaccggcggg ttcttccggg atcggaagcc
      121 catcccctgg taagatgaag ccatggaacc ggccgccact cgtcctcgcc catccttccg
      181 ccgcggggat gaggtcgcgc tcgcgatcga ccggatggcc tacggcggca gaggcgtggg
      241 gcgcatcgat gggtacgtcg tgttcgtccc cgataccgcc cccggggatc gtgtccgcgc
      301 gcgcctgtgg cgggtcaggc cggcgtacgc cgaggcggac ctggtggggg tcgaatctcc
      361 gtcggcgctg cggaccgtgc ccccctgccc gcacttcggc ccgtgcgggg gatgcgtctg
      421 gcagcacctc gggtacgctg accagctcag ggccaaggag agcatcgtcc gcgagagcct
      481 cgcccacctc gccggcctcc gcgacgtcga ggtcagaccg atcctcgccc cggccgaccc
      541 ctgggcctac cgcaacaaga tggagttcac gttccacccg gacgggacgc tcggcctcca
      601 ccggcggggg gcgttcgacc agatcgcgcc gatcaacacc tgcctgatcc aatccgctgc
      661 ggccaacgag gccctccggg tggtgcggga ctgggccgac gcctcgggac tgtcccggta
      721 cgacgcccgc gcgcacaccg gcctcctccg gcaggtggtc atccgcgagg ggaagcgcac
      781 cggcgagatg atggtcgcgt tgatcacgac agccccggag gtgccggcag cccgcgacct
      841 ggccgcccgc ctcctggccg gtgggcggat caccagcgtc ctcctcgggg tgaacccggg
      901 cccgtcggac ggcctgcccc tggccggcgt ctcggtgctc gcgggacgcc cctacatcca
      961 cgagcatctc gccggcctcc ggttcaggat cgggctggag acgttcttcc agaccaacac
     1021 cgcccaggcg gagcaaatga tcgaagtggt ggacgccctc gccgggctgc ggggcggcga
     1081 gaccgccttc gacctctaca gcggggtcgg aacgttctcg ctggccctcg cacgccgggc
     1141 agagcgggtg tacggcctgg agatcgttgc ctctgccgtc gagaccgccc gggagaacgc
     1201 cgcgctcaac ggtatcggga acgccgagtt catctcgggc gatgtccgcc ggggcctgcc
     1261 ggatctgctc gcccgcgtcg gcccgccgca cctcctcgtg ctcgacccgc cacggagcgg
     1321 cgcggggagc cgggtcatgc gccgggtgat cgccgcggcc gccccgcgga tcatctacat
     1381 ctcctgcaac cccaccacgc tggcgcccga tctgggggac ctcgtccgcg cggggtacgt
     1441 catccgcacg gtccaaccgc tggatctctt cccgcacacc taccacgtcg agtgcgtcgt
     1501 gctgctggag cgaaacggcg cgccaaacgc ggagggagcg gcggcccagg catgacccgc
     1561 caggaagcgt tcctgccagg gtgggttcgg agcctggtgc ggggcgcagt tgtcgggatt
     1621 ctggtcgggg tgagcgtcgg agccgtcgtc tcagcgttcg tggtctccgc ctatgccatc
     1681 cttctctttt actatgcggc gccgagcgcg gcgctgctgg gcgcgctcca ggggctgtgg
     1741 ctgtatctgg agcgcgaccg ctgggcgagc cggagagagg tgctcctttt cggcgcggtg
     1801 tcgggagctg ccctcggcct gctcgggttc gcgcctatct tcgttcaaac gaacgtgctc
     1861 ctccactggc gcgatattgt gctcttgctc tgcgccgcga tcgccggcgg gggggctgcc
     1921 gggcgggtga acgcttcctt ctttgtttcg tacgctcggc cgcaggtgtc gccggggctc
     1981 cggaggcgtc tcgtcgcggg ggggctcatc gtcctctcga ttgggctctt cgagtacttc
     2041 cagtacgggc cggccgtggc acgcaagctg cccgtcccgg cgttatccga gcgtgccgtc
     2101 cgcaatctcc cctccgggca tgcgcgcggg gcgcgctggg tcggttgcta cgagtacaac
     2161 ggcgtcttct ttgaaggcag cggggtcgtc gggaccgcag ggggagcgat ggtgttcgtt
     2221 cagaagggcg gggccctcca aatcgacgac atagggcacg ggaggaccct gaggggaggg
     2281 atcgatgcgg atgggcgatt ttgggcggga acccagggcg aagaacggga tggatctctc
     2341 ctgcgattgc tcgaaaaagg aagattcttg gatcgcgatc gtttcaagtt ttcctttcgg
     2401 gtcagcctgg tgaaaaacgg gtccgttgcc aacaccaccc gggaagacgg tgcgggacac
     2461 cgcgtctcct gctaggttgg ctctcctccg gctgtcctga cgagcagcgg catgagatcg
     2521 acgccccgca gcatcccgag gcttccgtgc gcgcaggccg cttcgctcca ctcccggctc
     2581 ccgtcggagg ggacaccggc gccggccagc aatagcggca cggggtcgtc ggagtgtcgg
     2641 tggagggcgc acggcgtggc gtggtcggcg gtgacggcca ggacgacctc ctccaggttg
     2701 atcccgggca ggagcgtcgc gaagaacgcc cggtcgatca cctcgatcac cgcccgcttg
     2761 gcttcacaat cgccgtcgtg gcccggttcg tcggggcctt tgagatggat gtagagcccg
     2821 cccgagcgcg tgatctcctc cgccgcggtc ctcgcccacg ccgcgtagac ccccgccttg
     2881 tccgccccgc ttggggggac cgggatcacc cgcatcccgg tgagctgggc gatcccccgc
     2941 tcgaccggca tctccacgaa gcacccaaac tgcaccccga agcgctcggc gatcgggggc
     3001 atcgagggaa ggtggtcccc ggcatcccgg acaagaatca agttggcggg gggccggcct
     3061 cgggcccgcc ggcgcgcgtt gacggggtgc cggtcgagca ggtcgtgcga gcgccgggtg
     3121 aactcgttga ccagggcggc ggcggtcctg gcttcttcgc tggggtccag cggcgtgcac
     3181 tcctccgcca cgtctcccgc ctgctcgcgg gccacgccga ggccttcgac acgtccatac
     3241 gcggggtcgg tgttgctgat cttcgcggag agccgcccgc cctgccggcg gatcaccacc
     3301 ccgcaccggt gcccgatgct ggcccgcacc tccagatcgg ccggcgcggc ttcgagccgc
     3361 aggtgccagg tcaccgcgtc cgcgagcgcg cccgcctcct cggcggtgag atcccgcccg
     3421 gcccggcgat cgaggatgcg caggccgtcc ccggcggtcg cgaagttccc gcggagcgcc
     3481 aggtcgccat cccgaaacgc catccccgcg cccacggcct cgaacacccc gcggccggtg
     3541 tggtacgtaa acggatcgta ccccaggatg gcggtgaccg ccacgtcgga ctccggagcg
     3601 atcccctggc ccacggtctg caccaggccg gtgcggccac ggcgcgcgag ggacgtgagt
     3661 tcggggatgc gggcggcgcc cagcggcgtc cggccgccga gcgcggggac aggccggtcg
     3721 cccagcccgt ccaggatcac gtagaggatc cgcctcatcc ccttcgcagg cgggcgagca
     3781 gatcggggct gaaggtcccg cggcaggtct cctccacggg cccggtcagg cgcaggtcgt
     3841 agtccccgcc gacgtgcacc cgcagaatgc ccccgggggt gtgcacggcc acgtctcgcc
     3901 cggtgaagcc atggcggacg caggccgcgg cgaccgcgct gctgctgctc ccggacgaca
     3961 tggtctcccc ggcgccccgc tcccagatgc ggatcgccac gctcctcgtc gagtccaccc
     4021 gcgcgaactg gacgttcgtc cggttcggga acatcggatg gtgctccagg cttgcgcccg
     4081 cgtgcgcgag gtcgacggac gcgaggtcat cgacgaagac cacgcagtgc gggttgccca
     4141 cggacacggc ggtgacccgg aaccgctggg ggcccaccgc cacctcttcg tccaccacct
     4201 cccggtcggg accggtcacg gggatcgcgc ggctgcgaaa cgtggcccgg cccatcccca
     4261 cggtgatgct gcgcacctgc cggccgcgga gctggaggat gacagagacc acccccccgc
     4321 gggtctcgat gtcgaagatc cggcggcggg tgtacccgtg gtcccacaga cacttggcga
     4381 aaatccggac cccgttcccg ctcttctccg cctcgctgcc atccgggtta aagatgcgaa
     4441 tgccgaaccc cgcgcggcgc gaccgctcga tcgtgaggat tccgtccgat cccacgccga
     4501 ggttacggtg gcagagcgcc cggaccaccg ggggcgagag gcgaaacgag aggctccggg
     4561 ggtccaccac gaggtagtcg ttgcccagcg catgggattt gacgtactcg ttccggtgga
     4621 gccgtcccat cccccgctac gccccccgcc cccggcgccg ttggcgctgg cggcggagcg
     4681 cgccttggat gatccggttg acgagctcgg gaaagtcgag tccggccgcg gcggcagcct
     4741 tggggaggag gctgcccggg gtcaggcctg ggatggtgtt tacctcaagc agcacgatcc
     4801 cgcctttgcg cgcgaacata tccacccggg acagcccctc gcagccgagg gcccggtggg
     4861 cgcgcacggc cagttgctgc gccttccggg ccagcgccgg cgcgatgcgc gccggaacga
     4921 tttcctcgct cgcgcccggc tcgtactggc ctgataatag aagaactccc gcctgggcac
     4981 gatctcgatc agcggcagcg gcgtcgggac gcccgtcttc ggatcctcga ggatggcgca
     5041 ggtgagctcc gtgccccgga ggtactcctc gaccagcacc acctcgccgt actcgagcgc
     5101 ccgctccacg gccggcacga gctccttcgc cgtgcgaaca atcgtcacac cgatgctcga
     5161 cccctgggcg ttgggcttga ccacgcaggg gaatcccagt tccttcgaga cgcgggcccg
     5221 gacctcggcc ttgttgcccg gccaggcgcc gccatccacc acgaccaacg cagggaacgg
     5281 cagcccgttg gcgaggccga cttggcggct gcgcagcttg tccatcgcga gcgcgctggc
     5341 cagcaccccg gaccccgtat acgggatccc gagcaactcg agcaggccct ggatcgtccc
     5401 gtcctcgccg tacgggccgt gcagcgcgat gaacgccaga tccacgctcc cgtccccgac
     5461 cgcttcctcg atgcggtggg gcacgagcgc cgtggaaccg gcggcctccg gcacggcccc
     5521 gaccccgggg gagcgccccg gtgcggcgag ccggaggagg tcgggacgcg ggagccactt
     5581 gccttcgcgg gtgatctcat aagggagcag ctcgtactgc tgcggatcgg ccgcctggag
     5641 cacctggagc ccgctggcca gggagacgtc ccgctcgggt gacggcccgc ccatcaagac
     5701 cgccacgcgc agccgtttcc taccgggggt cggtccacgc ttgatcggat caggcgccat
     5761 cgggtcacgg tcacgttcgg ggatggagga ccaatccctg ccttcgccgt acgatcaaag
     5821 gcatgtcgat tctcggcgtc atcttcgacc tcggcagcac gctcatccac cggaccggcc
     5881 tggaactcga gaaagagaag tgcgggaccc tggccggggt cgccgcggcc gagtggggct
     5941 acggggatcc ggaggccctg gccgcccggt tgctcgagat ccgtctcgac gcgtggcggc
     6001 ggtcggacca ggagctggtg gagagcgtgg ccaccgagtc gatcgcccag gcgttcagcg
     6061 aggcggggct ccccaccgat gcggcgacgc tgcagcgggc cgaagcggtg ttctttgagc
     6121 cggaggtccg ggccagccac ctctacccgg gggccgcgga caccctcgag acgctccacg
     6181 ccatggggct cagactcggc atgatcagca acgccacgag ccacaacctg atcctcgaca
     6241 tcacggcgaa gctggagatc gaggagtact tcgacccgct cgtctcctcg gccgggtttg
     6301 gatggatcaa gccgcacccg gccatcttca cccacgtcct cgacgcgtgg gggatcaccg
     6361 ccgcgtcggt cgtgatggtc ggcgataccc tccgcgccga catccatggc gcgcacacgg
     6421 tcggcatgcg gtcgatcctg gtggatatcg agccgaaccc agccaacgcg cggttcgacg
     6481 gtcccgccac cccgatggcc cgggtcacgg cgctccggga gatccccggc ctcatccgcc
     6541 ggtggggtac cccgcgggtg ccgcgaaagc gtccgcgggc taaacgcggc tgagcgcctg
     6601 gtcgaagtcc tcgagcagat cctggaggtc ctccacgccc accgagaccc ggatcaatcc
     6661 gtcggcgatc ccggccgcga ggcgcacctc gcgcggcatg ttgacgctcg tggtcgaggc
     6721 ggggtgggcg acgagggtgc tgaccccccc aag
//
LOCUS       DASQGH010000634         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_22603, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000634 DASQGH010000000
VERSION     DASQGH010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22603"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VEW91_12655"
     CDS             complement(<1..301)
                     /locus_tag="VEW91_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HYM92471.1"
                     /translation="MTPPDRYICIHGHFYQPPRENPWLEEVEIQDSARPYHDWNARVT
                     AECYAPNGASRILDGDQRIVEITNNYRRISFNVGPTLLWWLEGAAPVTYARIIAAD"
     gene            complement(313..1560)
                     /locus_tag="VEW91_12660"
     CDS             complement(313..1560)
                     /locus_tag="VEW91_12660"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HYM92472.1"
                     /translation="MAHPRVLGLILAGGKGERLYPLTKERSKAAVPFGSKYRIADFVL
                     SNFINSGIYSLYILVQYKSQSLIDHIRRGWRLGGVIDDHFIITVPPQMRWGETWYRGT
                     ADAVFQNINLIRDLNPQLVAIFGADHVYRMDLAQMIASHLERRADVSVAALPIPIETA
                     SGFGIMEAERDGRIIGWEEKPRDPRPIPDDPRRALSSMGNYIFTTDVLVEALIEDARR
                     STDHDFGRTIIPELHPYARVFAYDFLQNEIPGLRPSEERGYWRDVGTIEAYWQAHMDL
                     LGSAPALDLNNPGWPILSPSFADPAARILAGTVHDVQLGEGTVIHGATVRHSILGRGV
                     MIEQGAVIEDSIIMDNTVVGPNARVRRAIVDRFNVIEPGMAIGEGAEHLPDHAVLDPS
                     GITVLARGATRVRGAAPGAPARP"
     gene            complement(1565..>3102)
                     /locus_tag="VEW91_12665"
     CDS             complement(1565..>3102)
                     /locus_tag="VEW91_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HYM92473.1"
                     /translation="SPVLCEQLANAGYQEEVAAWLQQRLEMCRENQRYFTDTHQDHLA
                     GLCDYWRAFYEGARDHFDALGGDVLGAFRALADREAIELITCAATHGYLPLLSRDESL
                     DLQIGVAAATHTRHFGRPPRGIWLPECAYRPRYEWTPPVGPRSGKVRYRRRGVEEILA
                     MHGLEYFFTDTHLVRGGHALSAYRDYFPSLRAVTGPESPPFYSRRERTPYACYLVASR
                     GGTGQAAAVVRDPETTLQVWSRDVGYPGDEWYLDFHKTHFPGGLRFWRVTHPKSDLAD
                     KQPYVPERAEERLRAHAQHYAGTVRAILSRSAGEPGGPGVLCSPFDTELFGHWWYEGP
                     RWLAQVFGRLGAEGVAPITAGHWLDAHPARETITLLEGSWGEGGDHRVWMNKDTEWTW
                     EMVYKAEEDLWTFAASDRWRRTPLVRRIVEQLARELLLLESSDWQFLITTWSARNYAE
                     TRFAEHSADFTRLLDLAKRVQGGGSLSWDEEEYLKGKEAQDFCFPDLAGHLEAASRRP
                     GVE"
BASE COUNT          451 a         1051 c         1100 g          500 t
ORIGIN      
        1 ggtcggccgc gatgatgcgc gcgtaggtca ccggtgccgc cccttccagc caccagagca
       61 gggtggggcc gacgttgaag ctgatgcggc ggtagttgtt ggtgatctcg acgatgcgct
      121 ggtcgccgtc gaggatgcgc gaggcgccgt tcggcgcgta acactcggcg gtcacccgcg
      181 cgttccagtc atggtacggt cgggcggagt cctggatctc cacctcctcg agccaggggt
      241 tctcgcgcgg gggttggtag aagtgcccgt ggatgcagat gtagcggtcg ggcggggtca
      301 tagggcggcc gactaggggc gggccggcgc gcccggggcg gctccgcgga cccgggtcgc
      361 cccgcgcgcc agcacggtga tgcccgacgg atcgaggacc gcgtgatcgg ggaggtgctc
      421 ggcgccctcg ccgatcgcca tgcccggttc gatcacgttg aaccggtcga cgatcgcccg
      481 gcggacgcgc gcgttcgggc cgacgaccgt gttgtccatg atgatgctgt cctcgatcac
      541 ggcgccctgc tcgatcatca cgccccgccc gaggatcgag tgccggaccg tggccccgtg
      601 gatgacggtg ccctcgccga gctgcacgtc atggaccgtg ccagccagaa tgcgcgccgc
      661 cgggtcggcg aacgacgggg acaggatcgg ccacccgggg ttgttcagat ccagcgcggg
      721 ggcggagccc aacaggtcca tgtgcgcctg ccagtacgcc tcgatcgtgc cgacgtcccg
      781 ccagtacccc cgctcttcgg acgggcggag ccccgggatc tcgttctgca ggaaatcgta
      841 ggcaaagacc cgcgcgtacg ggtggagttc ggggatgatc gtgcgcccga agtcatggtc
      901 ggtgctccgc ctcgcgtcct cgatcagggc ttcgacgagg acgtcggtcg tgaagatgta
      961 gttgcccatc gaggagagcg cccggcgtgg atcgtccggg atggggcgcg ggtcgcgggg
     1021 tttctcctcc cacccaatga tccggccgtc ccgctcggcc tccatgatgc cgaacccgga
     1081 cgccgtctcg atggggatcg ggagcgcggc caccgagacg tcggcgcgcc gttccaggtg
     1141 cgacgcgatc atctgcgcta ggtccatccg gtacacgtgg tcggcgccga agatggcgac
     1201 gagctggggg ttcaggtcgc ggatcaggtt gatgttctgg aacaccgcgt cggcggtccc
     1261 acgataccac gtctctcccc accgcatctg gggcgggacg gtgataatga agtggtcgtc
     1321 gatgaccccc cccaacctcc agccgcggcg gatgtgatcg atgagcgact gcgacttgta
     1381 ttgcacgagg atgtagagcg agtagatgcc cgagttgatg aagttgctga gcacgaagtc
     1441 ggcgatccgg tacttgctcc cgaacggtac ggccgctttg ctgcgctcct tcgtcaacgg
     1501 gtagagccgc tcgcccttgc cgcccgccag gatcaggccc aggacgcggg ggtgcgccac
     1561 gcgatcactc caccccggga cgtcgggatg cggcctcgag gtgccccgcg aggtcgggga
     1621 agcagaagtc ctgggcctcc ttgcccttga ggtactcctc ctcgtcccac gacaggctcc
     1681 cccctccctg gacccgcttg gcgaggtcca gcaggcgggt gaagtccgcg gagtgctcgg
     1741 cgaaccgcgt ctcggcgtag ttgcgcgccg accacgtggt gatgagaaac tgccagtccg
     1801 aggactccag gagcagcagt tcgcgggcca gctgctcgac gatccggcgc accagcggtg
     1861 tgcggcgcca ccgatccgac gccgcgaacg tccagaggtc ctcctcggct ttatagacca
     1921 tctcccaggt ccactccgtg tccttgttca tccagacgcg gtgatcgccc ccctcgcccc
     1981 aggagccttc gaggagcgtg atcgtctcgc gggccggatg cgcgtcgagc cagtggcccg
     2041 cggtgatcgg ggccacgcct tccgccccta ggcgcccaaa gacctgggcc agccaccgcg
     2101 gcccttcgta ccaccagtgg ccgaagagtt cggtgtcgaa cgggctgcac agcacgccgg
     2161 gtccgccggg ctcgccggcg ctccggctga gaatcgcccg aaccgtgccc gcgtagtgct
     2221 gggcgtgcgc ccgcaggcgc tcctccgccc gctcgggcac gtacggctgc ttgtccgcca
     2281 gatcgctctt ggggtgcgtg acccgccaga atcgcagccc ccccgggaag tgcgtcttgt
     2341 gaaagtcgag gtaccattcg tcgccgggat agccgacgtc ccgactccag acctgcagcg
     2401 tcgtctcggg gtcccgcacg accgccgcgg cctgccccgt gccgccgcgc gacgcgacca
     2461 ggtagcaggc gtacggggtg cgctctcggc ggctgtagaa cgggggcgac tccggccccg
     2521 tgacggcgcg gaggcttggg aagtaatcgc ggtaggctga gagcgcgtgg ccgccgcgca
     2581 ccagatgggt gtccgtgaag aagtactcga ggccgtgcat cgccaggatc tcctccaccc
     2641 cgcgccggcg gtagcggacc ttgccgctgc gcgggccgac ggggggcgtc cactcgtacc
     2701 ggggacggta ggcgcactcc ggcagccaga tccccctcgg ggggcgaccg aagtggcggg
     2761 tgtgggtggc ggcggccacg ccgatttgca ggtccaggct ctcgtcgcgg ctgaggaggg
     2821 gtaggtaccc gtgcgtcgcc gcacaggtga tcagctcgat cgcctcccgg tctgccagcg
     2881 cacggaatgc gccgaggacg tccccgccca atgcgtcgaa gtggtcccgg gcgccttcgt
     2941 agaacgcgcg ccagtagtcg cacagcccgg cgaggtgatc ctggtgcgtg tctgtgaaat
     3001 atcgctggtt ctcccggcac atctccaggc gctgctggag ccaggcggcg acctcttcct
     3061 ggtaccccgc attggccagc tgctcgcaga gcaccggcga ca
//
LOCUS       DASQGH010000635         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_53743, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000635 DASQGH010000000
VERSION     DASQGH010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53743"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VEW91_12670"
     CDS             <1..1344
                     /locus_tag="VEW91_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYM92474.1"
                     /translation="DDLATRLIYLGLIVLGLNLIVWAWGSRTVSVTAWSELLGGLCML
                     AFGGGIEFRALQRARRLVILSQISAALQRKEVVENLLDDVLHRAGDLLQLHSGWAFLR
                     NPRSGAFEIAAAYQLPPPLERDHRAPMQGSCRCLDMLEANELTQPVDIVHCVRLEGVG
                     AQAQHASVPLRSSTGTIGLMNLVLPSGRLFSQRDLALLAAVGGQVGLAIEKTRLLEEL
                     REKEQIRSDLIKKLLTAHEDEQRRIARELHDETGQSLTALILQTEMLRTLAEEGKPTS
                     VAELDRLQALAENTLEEVRKLIYDLRPTILDDLGIAAALRWYVHHQLEPRGLSVDLHL
                     RLGEARLDPMLETALFRIAQEALWNVVKHAEATHVDVALTLNNGRLRLRVKDNGRGFR
                     PRQRGSAGPLAGGMGLGGMEERAALLGGTVTVTSTAGAGTEVVAELPLPKPVKES"
     gene            complement(1352..1855)
                     /locus_tag="VEW91_12675"
     CDS             complement(1352..1855)
                     /locus_tag="VEW91_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYM92475.1"
                     /translation="MTELLSHPVSCLEHLKAYLGGEGVAYTVDEHPSRYTAQELAQIE
                     HVSGRLIAKVVMLIVDGKPVMVAVPGVAKVNFAAVQKALAAKDVRLALENEFEHLFPD
                     CEVGAMPPFGNLYGVPVLVDEALTRDPVILFNAGTHGRTITMTYADYARLVRPAIGAF
                     GKEPVGV"
     gene            2141..2812
                     /locus_tag="VEW91_12680"
     CDS             2141..2812
                     /locus_tag="VEW91_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYM92476.1"
                     /translation="MLVRDRMTSDVLTVAPDTPLEHAMTLMRSRHIRRLPVLQHGRLV
                     GIVTWTDLMRAQPSPASTLSRWEIPALLEKAQVGEVMTPSPETVTPGTPLEEAAATMR
                     QRKIGGLPVVANGTLVGIVTESDIFDAFIALLGVRTPSYRITLDVDDSAAALPDIAAA
                     FRLLGLRLYSVSTYAAARGRLRVVVRVERAIPLPSLVDALRERGLETVHAADGTKVII
                     EAARV"
     gene            complement(2895..3830)
                     /locus_tag="VEW91_12685"
     CDS             complement(2895..3830)
                     /locus_tag="VEW91_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HYM92477.1"
                     /translation="MAPSRYFPMDTVHFTWDVRHEPAMAIDTGDVVVVRTRDVSDNQI
                     TPASTASVLATLNWERLYPLAGPIYVNDAQPGDTLAMEILDIHAEGWGWTAILPGYGL
                     LTDDFKEPYLRIFDLTNGDVAYLRDDIAIPIEPFFGTMGVCPAGASTQAVMPPGTFGG
                     NMDTRQLTRGTTLYLPVHVEGALFSCGDAHAAQGDGEVCVTGIEAPMYAALRFTIQKR
                     RRIPAPQFLTPGPLVPRVNQGGWYGTTGVGPDLYSAAQDAVRAMIAHLSETYRLSAED
                     AYVLSSLCVDLKISEIVDAGQYIVSALLPLAVFRQ"
     gene            complement(3869..5140)
                     /locus_tag="VEW91_12690"
     CDS             complement(3869..5140)
                     /locus_tag="VEW91_12690"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYM92478.1"
                     /translation="MAVKAEVAVPREDAWDIARQQFSLAADHLPLKRGIRELLNRTER
                     ELTVNFPVRMDDSSVRVFTGYRVHHSTVLGPTKGGIRYHPAVTLNEVRALSMWMTWKC
                     AVAGLPFGGAKGGVVVDPKVLTRSELERLTRRYATEITVFMDPQGDIPAPDVGTNPQT
                     MAWIMDTYSMHRGYSVPSVVTGKPLEIGGSQGRLEATGRGVMISARETCRRLGMSLAG
                     ARVAVQGFGNVGAVAAYLLQDQGSVIVAASDSAGGVYNPKGLNARELEAFKREHGTLA
                     GFPGATRVSNEQLLELPCEILVPAALEGQVTADNAPRVKAHLIIEGANGPLTPKADEV
                     FQDRGITVVPDILANAGGVIVSYFEWVQGLQQFFWTEEEVNQNLERIMMRSIAQVVET
                     SQEKRTTLRTAALVRAIDRVAEALMTRGIYP"
     gene            complement(5249..5914)
                     /locus_tag="VEW91_12695"
     CDS             complement(5249..5914)
                     /locus_tag="VEW91_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYM92479.1"
                     /translation="MIDYEGYVFADRAEAGTRLGAVIGDRLGPEEDAVILGLPRGGVV
                     VAREVARLTRRPLDFVVVRKIGAPENEEVAIGAVGEDGEPVLDKATIERYGISPMYVS
                     MATARVRAEVQRRVEAYRQGPRPSLQGKTAVLVDDGIATGYTVEAAVETVRRWGAARV
                     VVAVPVAPPEAVARLRPYADDVIALHMPRFFMAVGQFYADFQQVTDDEVKAILTRAAT
                     LAA"
     gene            6048..6518
                     /locus_tag="VEW91_12700"
     CDS             6048..6518
                     /locus_tag="VEW91_12700"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HYM92480.1"
                     /translation="MKRSLAFALAVAAVIIGLPGARADLPGRIPVVMLDQPSVPVRLT
                     EIQSVVSLLPADHTLATVWFVRGVMTNVTRKRIVATETTWTLVNADGEIVGAIRTLYE
                     NPIAPGERRQTVIGVLTDLLHKRGERVIGLVRRVRFEDGSFWTAPPAELAKVPR"
     gene            6651..>7087
                     /locus_tag="VEW91_12705"
     CDS             6651..>7087
                     /locus_tag="VEW91_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYM92481.1"
                     /translation="MATVRLRRRDAYRWEIPLEYKLGMRVPGLVYADDALLAQVQEEH
                     ALEQVANAATLPGIVRWAIAMPDIHWGYGFPIGGVAAMRLDDGVVSPGAVGYDINCGV
                     RLLATSLVEGEIRPHLEELAGLLFHNIPSGVGAHGRLRLHRSEL"
BASE COUNT         1178 a         2442 c         2298 g         1169 t
ORIGIN      
        1 gacgacctcg cgacgcggct gatctacctc ggcttgatcg tcctggggct caatctcatc
       61 gtctgggcct ggggatcgcg gacggtcagc gtcacggcgt ggtcggagtt gctcgggggc
      121 ctctgcatgc tcgcgtttgg agggggaatc gagttccggg cgctgcagcg ggcgaggcgg
      181 ctggtgatcc tcagccagat cagcgcggcc ctgcagcgca aggaggtggt cgagaacctg
      241 ctcgacgatg tgctgcatcg ggcgggcgac ctgctgcagc tccactcagg ctgggcgttc
      301 cttcggaatc cccgaagcgg ggccttcgag atcgccgccg cgtaccagct ccctcccccg
      361 ctcgagcgcg accatcgcgc gccgatgcag ggctcgtgcc ggtgcctcga catgctggag
      421 gccaacgagc tcacgcaacc ggtggacatc gtgcactgcg tgcgactgga gggggtgggt
      481 gctcaggccc agcacgccag cgtgccgctt cgcagttcca ccggcaccat cggcctgatg
      541 aacctggtgc tcccgtcggg ccgcctgttc agccagcgcg acctggccct cctggccgcg
      601 gtcggcggcc aggtcggcct ggccatcgag aaaacacggc tcctcgagga gctgcgcgaa
      661 aaggagcaga ttcgaagcga cctgatcaag aagctcctca cggcgcacga ggacgagcag
      721 cggcgcatcg cccgcgaact gcacgacgag accggccagt cgctgacggc gctcatcctg
      781 cagaccgaga tgctccggac gctggcggag gaggggaaac cgacgtcggt ggcggaactg
      841 gaccgtctcc aggcgctggc ggagaacacg ctcgaagagg tccgcaagct gatctatgat
      901 ctccgcccca cgatcctgga cgatttgggg atcgccgccg ccctacgctg gtatgtccac
      961 caccagctgg agccccgcgg gctttcggtg gacctccacc tccggctggg cgaagcccgg
     1021 ctggatccga tgctcgagac cgccctgttc aggatcgccc aggaagcgct ctggaatgtc
     1081 gtcaagcatg cagaggccac tcacgtggac gttgcgctca ccctcaacaa cggccggttg
     1141 cgtttgcggg tgaaggacaa cggccgcggc ttccggcccc ggcagcgcgg atccgccggc
     1201 ccattggccg gtggcatggg actcggggga atggaagagc gggccgcgct tctcgggggc
     1261 acggtcaccg tcacctccac cgctggggcc ggcaccgaag tcgtcgccga gttgcccctc
     1321 ccgaagccgg tcaaagaatc ctgacggctc tctacactcc caccggctcc tttccgaacg
     1381 cgccgatcgc cggccggacg agtctggcgt aatccgcata ggtcatcgtg atcgtccgcc
     1441 cgtgggttcc tgcgttgaac aggatcaccg gatcacgggt cagagcctcg tcaacgagga
     1501 cgggcacgcc gtagaggttg ccgaacggcg gcatggctcc gacctcgcag tcgggaaaca
     1561 gatgctcgaa ctcgttttcg agcgccaacc tgacatcttt cgccgccagc gccttctgga
     1621 ccgcggcgaa gttgactttg gcgacgccgg gcacggccac catgacgggc ttcccgtcca
     1681 cgatgagcat cacgaccttc gcgatcaggc ggcccgaaac gtgttcaatc tgcgccagct
     1741 cctgggcagt gtaccgagac gggtgctcat ccaccgtgta agccacccct tccccgccca
     1801 ggtatgcctt gagatgttcc aggcaggaaa ccgggtgtga cagtaactca gtcatacgcg
     1861 cccacctcct ctcgcctcca acatagcccc caacgatccc cggagatatc gggtcgctac
     1921 ccgattctcg cgtgggagcc ccaggggggc cgggcccctg gcagcctcca agatcggtcc
     1981 ctgccaggag ccgaataccg cctcgacccg atggggtccc ggaggggcgg gcgctatcct
     2041 caatttgaac cttggtccgt agccccgaac cgtcagatcg cggcgtatgg aggcggcggg
     2101 gcagcagcgt gtttggccgc gcatggaggg gggaagagag atgctggttc gcgaccggat
     2161 gacctccgac gtcttgaccg tcgctcccga cacaccattg gagcacgcga tgacgctgat
     2221 gcgaagccgg cacattcggc gcctgccggt gcttcagcac ggccgtctcg tagggatcgt
     2281 cacctggacc gacctgatgc gcgcccaacc ctcaccggcc tcgaccttga gccggtggga
     2341 gatccccgcc ctcctggaaa aggcgcaggt aggcgaggtg atgaccccgt cccccgaaac
     2401 cgtcaccccc gggaccccgt tggaggaagc cgccgccacc atgcgccagc gcaagatcgg
     2461 cggactgccc gtggtcgcga acgggaccct ggtggggatc gtcaccgaga gcgacatctt
     2521 tgatgcattc atcgccctgc tgggggtgcg gacgccctcg taccgcatca ccttggacgt
     2581 cgacgacagc gcggccgccc tgccggacat cgcggccgcc ttccgcctcc tcgggctccg
     2641 gttgtacagc gtgtccacct atgcggcggc gcgggggcgc ctgcgcgtgg tcgtgcgggt
     2701 cgagcgggcg atcccgctcc cgagcctggt ggatgccctg agggagcgcg gtctggagac
     2761 cgttcacgcc gcggacggca ccaaggtgat catcgaagcc gcgcgcgtgt agggacgggc
     2821 gacgcagcgg gaccaggcac acccttccgg ttcagccatc gcgcccgcgc cggccggatc
     2881 ggccggtcag gcgtctactg gcgaaacacc gccaacggca gcagcgcgct cacgatgtac
     2941 tgtccggcgt caactatctc cgagatcttc aaatccacac agaggctgct gaggacgtag
     3001 gcgtcctcag ccgagagtcg gtacgtctcc gacaggtgcg caatcatcgc ccgcaccgcg
     3061 tcttgggcgg ccgaatagag atccggcccc accccggtcg taccgtacca tcccccctga
     3121 ttgacccgcg ggacaagcgg cccgggcgtg aggaactgcg gggcggggat ccgccggcgc
     3181 ttttggatcg tgaagcgcag cgcggcatac atgggcgcct caatgccggt gacacacact
     3241 tcgccatcac cctgcgccgc gtgggcatct ccacagctga acagggcacc ttccacgtgc
     3301 accggcaggt agagcgtcgt cccgcgggtc agctggcggg tgtccatatt cccaccgaac
     3361 gttcccggcg gcatcacggc ctgcgtgcta gcgccggccg ggcagacgcc catggtgccg
     3421 aagaagggct cgattgggat ggcgatgtcg tcgcgcaagt aagcgacgtc cccattggtg
     3481 aggtcaaaaa tcctgaggta cggctctttg aagtcgtcgg tcaacagccc gtagccgggg
     3541 aggatagcgg tccatcccca tccctcggca tggatgtcga ggatctccat ggcgagcgta
     3601 tcgcccggct gagcatcgtt cacgtagatc gggccggcca aaggatagag gcgctcccag
     3661 ttcagtgtgg ccaagaccga ggccgtcgac gccggcgtga tctggttgtc gctcacgtcg
     3721 cgcgtgcgca cgaccaccac atcgccggtg tcaatcgcca tcgccggctc gtgccggacg
     3781 tcccacgtga agtggaccgt gtccattgga aagtagcggc tcggcgccat ggcaagatca
     3841 cccctgcgtg gcgttggctc gggacgggct acgggtagat cccgcgggtc atcagcgcct
     3901 ccgcgacgcg atcgatcgcc cggacaagcg cagccgttcg gagcgtggtc cgcttctcct
     3961 ggctcgtctc gacgacctgg gcaatgctgc gcatcatgat ccgctcgagg ttctggttga
     4021 cctcctcctc ggtccagaag aactgctgca atccctgcac ccactcgaaa tacgaaacga
     4081 tcaccccgcc ggcgtttgcc aggatatccg ggaccacggt gatcccccgg tcctggaaca
     4141 cctcgtccgc tttgggggtg agcgggccat tcgccccttc gatgatcaga tgcgccttga
     4201 ccctgggcgc gttgtcggcg gtcacctggc cctccaaggc ggccgggacc aggatctcgc
     4261 aggggagctc gagcaactgt tcgttggaca cccgagtggc tccgggaaag cccgcaagcg
     4321 tgccgtgctc acgcttgaac gcctccagct cgcgggcgtt gagccctttc gggttgtaga
     4381 cgccccccgc cgaatcgctg gcggcgacga tgacgctccc ctgatcctgc aagagatagg
     4441 ccgccacggc cccgacgttc ccgaagccct ggaccgcaac tcgggctccg gccagcgaca
     4501 tccccaagcg ccggcaggtc tcccgggcgg agatcatgac cccgcggccg gtggcttcga
     4561 gccgcccctg ggagccgccg atctcgagcg gcttcccggt cacgaccgaa gggaccgagt
     4621 atccccggtg catgctgtag gtgtccatga tccaggccat cgtctgggga ttcgtcccga
     4681 catccggggc ggggatgtcg ccctgcgggt ccatgaagac ggtgatctcg gtcgcatagc
     4741 ggcgggtcag ccgttcgagt tcgctccgcg tcaagacctt gggatcgacc accacgccgc
     4801 ccttggcgcc gccgaacggg agcccggcca ccgcgcactt ccaggtcatc cacatggaga
     4861 gcgcccgaac ctcgttgagg gtcacggccg gatgataccg gatcccaccc ttggtgggcc
     4921 cgagcacggt gctgtggtgc acgcggtatc cggtgaagac gcgcacagac gaatcgtcca
     4981 tcctcaccgg gaagttgacg gtcaactcgc gttccgtccg gttgagcagt tccctgattc
     5041 cccgcttgag cggaaggtga tccgccgcaa ggctaaactg ctgccgcgcg atgtcccacg
     5101 catcctcgcg cggtaccgcg acctctgcct tgaccgccat tgctcttccc cccccgcgtg
     5161 ccgacgcgga caccgcgcgg cacagagact ccttcaggat tttgccccat ccgcctttct
     5221 cctgcagcct acaagctgta gaccagcatc acgccgcaag tgtcgcggcc cgggtgagga
     5281 tcgccttcac ctcgtcatcg gtcacctgct ggaaatccgc gtaaaactgc ccgaccgcca
     5341 tgaagaacct cggcatgtgc agggcgatga catcgtcggc gtacgggcgc agccgtgcga
     5401 ccgcctcggg gggcgcgacc gggacagcca ccacgacgcg cgctgcgccc caccgcctga
     5461 ccgtctcgac agccgcctcg accgtgtacc cggtggcgat gccatcgtcc acaagcaccg
     5521 cggtcttccc ctggagggac ggccggggtc cctgccggta tgcctccacg cggcgttgga
     5581 cctcggcacg cactcgcgcc gttgccatcg atacgtacat cggagagatg ccgtaccgct
     5641 cgatcgtagc tttgtcgagg acgggctccc cgtcctctcc gacagccccg atcgcgacct
     5701 cctcgttctc cggcgcaccg atcttccgga ccacaacgaa atcaagcggc ctgcgggtca
     5761 gccgggccac ctctcgggcg accaccacgc cgccgcgcgg gagaccaaga atcaccgcat
     5821 cttcctcggg gcccagccga tctccgatca ccgctcccaa ccgggtccca gcctcggccc
     5881 ggtcagcaaa cacataaccc tcgtaatcga tcatccctag gccctccatc atgaggatag
     5941 ccgcccaccc cgctggcgcc catcgggccg cgacccgatg gctgattggc cggccacccg
     6001 atgctgcccg gctcgaatca cgctacccta taggagggga gaccacgatg aagcggtccc
     6061 tggcgttcgc gttggcggtg gcggccgtca ttatcggatt gccgggtgca cgggcggatc
     6121 tgcctgggcg gatcccggtc gttatgctcg accagccgag cgtccccgtc cgcttgaccg
     6181 agatccaatc ggtagtttcg ttgttgcccg cagatcacac gctggccacc gtctggttcg
     6241 tgcgaggcgt gatgaccaac gtgacccgga agcgaatcgt cgcgaccgag acgacctgga
     6301 cgctggtgaa cgcggatggg gaaatcgtcg gggcaatcag gacgctctac gagaacccga
     6361 ttgctcccgg ggagcggcgc cagacggtca tcggcgtgct gacggacttg ttgcacaagc
     6421 gtggagaacg cgtcatcggc ctggttcggc gtgtgcggtt tgaggatgga tcattctgga
     6481 ccgccccgcc ggcggagttg gcgaaggtgc cgaggtgatc acggcggtgg ggaatcgacg
     6541 gcgtgagccc gttgcgacat gcctcgcact caagccccgc cggaatggta cgatggaggt
     6601 gtcggttgga gcagtgattg gcatggggta ctccgaggtg aaccatggcc gtggcgacgg
     6661 ttaggctgag acgacgcgac gcatatcgtt gggagattcc gctcgagtac aaactcggga
     6721 tgcgggtgcc gggcctcgtc tacgccgacg acgctctcct cgcccaggtc caggaggagc
     6781 acgcgctcga gcaggtcgcc aacgcggcca cccttccggg catcgtacgc tgggcgatcg
     6841 ccatgccgga tatccactgg gggtacggct tccccatcgg cggggtcgcc gcgatgcggc
     6901 tcgacgacgg cgtcgtctct ccgggagcgg tcggctacga catcaactgc ggcgtgcggc
     6961 tcctggccac cagtctcgtc gaaggggaga tccggcccca ccttgaggag cttgccggcc
     7021 tgctgttcca caacatcccc tccggagtcg gcgcccacgg ccgcctccgg ctccaccgct
     7081 ccgaact
//
LOCUS       DASQGH010000636         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_11288, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000636 DASQGH010000000
VERSION     DASQGH010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11288"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VEW91_12710"
     CDS             complement(<1..611)
                     /locus_tag="VEW91_12710"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HYM92482.1"
                     /translation="MRAVATVAGVFIIVGILWDAFETVVLPRRVSRRLRFVRLFFAPL
                     WDAYAALMRRIPPSGRRENYLGYFGPISIIVLLAAWAAGLILGFAVLIWGLGSRLSSP
                     DGNPTFGIDLYMSGTTFFTLGLGDVTPQAPAERVVAVAEAGIGFGFLALVISYLPVLY
                     QAFSRREVRITLLDAWAGSPPAAVELIRRLGAYDHMAALGAFLT"
     gene            complement(608..1564)
                     /locus_tag="VEW91_12715"
     CDS             complement(608..1564)
                     /locus_tag="VEW91_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYM92483.1"
                     /translation="MGTRRHRRAWSQATEGLVYGTLGVLGFSLTLPATRVAVSQLDAT
                     LVGLGRAVVAGGLALVVLGVRGEAPPPRRLWVRIGIVAAGVVLGFPLFSALALRQLTS
                     AHSAVIVGLLPAATAVMAVVRAGERPTIGFWIACACGLASVLAFAASEGAGTIQGADV
                     LVLSAVLLGALGYAEGGGLSREIGGWRVICWALVASLPITAPAMAVAALRSGVSAGPA
                     AWAGFAYVSVVSMFLGFFAWYRGLAAGGVARIAQVQLLQPILTLVWSALLIGEQINAT
                     TIAAAILVLASVAASQRAAGPSLPSTPAIACPVEEATQRGTP"
     gene            1761..2573
                     /locus_tag="VEW91_12720"
     CDS             1761..2573
                     /locus_tag="VEW91_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HYM92484.1"
                     /translation="MRLGEMTWTEVQEARAAGGTAVIPLGSVEEHGPHAPVGDYLIID
                     EIAARAAEATGDVVAPIIPYGYSEYFRNYPGTITLRESTLDAVLTDTIDCLLGHGFPR
                     LAIFNGHAGNTGVVEHVTRRFRRARGLVIPSIAPFQLVQAPEVVERVYGARVELGHGG
                     EPVGSLMMHLRPDRVKISRAGAWGRRRVFGCPTDGLGAIRVEGMRVAVPLDMEDVAPP
                     ETGSLSDPHLGTPERGRALFEHAVESCARFLRWWRGVDPYLGNGRPLAGGRA"
     gene            2665..3024
                     /locus_tag="VEW91_12725"
     CDS             2665..3024
                     /locus_tag="VEW91_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYM92485.1"
                     /translation="MRIERRNKTSLGRKRRRDFDRSLLNLRDLGMPRWAIEYYCRVAR
                     VAGLPPHMVVCHVAVVVAGRQLHAPTPPEQAVKPAVDGESRPEEDSSNLAILRLVQAD
                     TARAEPEPRPRRRSRTP"
     gene            complement(3021..>3168)
                     /locus_tag="VEW91_12730"
     CDS             complement(3021..>3168)
                     /locus_tag="VEW91_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HYM92486.1"
                     /translation="GLLLLFRPATGALAVVWLIGTYALVFGVLLLVLGFRLRGYAGQK
                     PALA"
BASE COUNT          539 a         1098 c         1141 g          390 t
ORIGIN      
        1 gtgaggaacg cgccgagcgc ggccatgtga tcgtaggccc ccaggcggcg gatgagctcg
       61 accgccgccg gcggggaccc cgcccaggcg tcgagcaacg tgatccgcac ctcgcggcgg
      121 gagaaggcct ggtagagaac cggcaggtac gagatgacga gcgccaggaa gccgaacccg
      181 atgccggcct ccgcgaccgc cacgacccgc tcagccgggg cctggggagt cacgtccccg
      241 aggccgaggg tgaagaaggt cgttccgctc atgtacagat cgatgccgaa ggtcggattg
      301 ccgtcggggc tcgacaggcg cgaccccagc ccccatatca gcacagcgaa cccgaggatc
      361 agcccggccg cccacgcggc cagcagcacg atgatcgaga ttggaccaaa gtaccccagg
      421 tagttttctc gccggccgct gggcgggatc cgccgcatca acgcggcgta cgcatcccac
      481 agcggggcaa agaagagcct gacgaacctg aggcgccgcg agacccgccg cggcagcacc
      541 acagtctcaa aggcatccca gaggatgccg acgatgatga acacaccagc aacggtagca
      601 acggctctca tggcgtcccc ctctgggtgg cttcttcaac cgggcaggcg atcgcggggg
      661 tcgagggtaa ggagggcccc gcggcgcgct gtgaggcggc gacgctcgcg agaacgagga
      721 tggcagcggc gatggtcgtc gcgttgatct gctcgccgat caggagggcc gaccacacga
      781 gggtcaggat cggctgcagg agttgcacct gggcgatcct ggcgaccccg ccggcggcaa
      841 gcccccggta ccaggcgaag aatccgagaa acatgctcac gaccgacacg taggcaaatc
      901 cggcccaggc tgccggtccc gcgctgaccc cggagcggag cgcggcgacc gccattgccg
      961 gcgcggtgat cgggagcgac gcgacgagcg cccagcagat cacccgccac ccgccgatct
     1021 cgcgggacag ccctccccct tcggcatagc cgagcgcgcc aaggagcacc gcgctcagca
     1081 cgagcacatc ggccccctgg atcgtccccg ctccttcgga tgcggcgaat gccagcacgg
     1141 acgcgagccc gcacgcgcag gcgatccaga agcctatcgt gggccgctcg ccggcgcgca
     1201 caacggccat caccgcggtg gctgcgggca ggaggcccac gatgaccgcg ctgtgcgcgg
     1261 acgtcagctg acggagcgcg agcgccgaga agagcgggaa ccccaagacc acacccgccg
     1321 ccacgatccc gatccgcacc cacaggcgcc ggggcggcgg ggcctcgccg cggacgccga
     1381 ggacaaccag cgcaagcccg ccggccacca cggcgcggcc gaggccgacg agcgtggcgt
     1441 cgagctggct gacggcgacg cgggtggccg ggagggtgag gctgaatccc agcacgccga
     1501 gcgttccgta gacaagtcct tcggtggcct gcgaccacgc gcgacgatgc cggcgggtcc
     1561 ccatgtcact gggagaacgc cggcggcggg tcccgcgatc ccgccgcaca caaggttcga
     1621 tcattcgatc atccgcgccg caaagggggc ccgaacggtt ttccgcgccg gccgaccgag
     1681 aattgtcgca ggggtgagcg agaggcggaa ggaatggagg ttccgacgtg acgccggtgt
     1741 gcttgagggg gggaccgatc atgcgacttg gggagatgac ctggaccgag gtgcaggagg
     1801 cgcgcgccgc agggggcacg gccgtgatcc cgctgggttc cgtcgaggag cacggcccgc
     1861 acgccccggt cggcgattac ctcatcatcg acgagatcgc cgcccgcgcg gcggaggcga
     1921 ccggggatgt ggtcgctccg atcattccct acggctattc cgagtacttc cgcaactacc
     1981 ctggcaccat cacgctgagg gagtcgaccc tcgacgccgt gctcaccgac acgatcgact
     2041 gcctgcttgg ccacgggttc ccccgcctcg cgatcttcaa cggccacgcc gggaatacgg
     2101 gggtcgtcga gcatgtcacg agacgcttcc ggcgcgcgcg gggcctcgtg atcccctcga
     2161 tcgcaccgtt ccagctggtg caggccccgg aggtggtgga gcgcgtctac ggggccaggg
     2221 tcgagctggg gcacggcggc gagccggtgg gctcgctgat gatgcatctg cggcccgacc
     2281 gggtcaagat ctcccgggcg ggcgcctggg ggcgccggag ggtgttcggc tgccccaccg
     2341 acggcctcgg cgccatcagg gtcgagggca tgcgcgtggc ggtccccctc gacatggagg
     2401 atgtcgctcc gccggagacg gggagcctat cggatcccca tcttggcacg cctgagcgcg
     2461 ggcgggcgct gttcgagcac gcggtggagt cgtgcgcgag gttcctgcgc tggtggaggg
     2521 gggtggaccc gtatctcggc aacgggcgcc cccttgcggg cggccgcgcg taatgcgcgt
     2581 cccacgccgc ctgggacgcc cacgggtcgg ggagatcggg cggacgtcta ggggggtagg
     2641 gttgcgggca tatagtgggt acccatgcgg atcgagcgcc gcaacaagac ttcccttggc
     2701 cgcaagcgcc gccgggactt cgatcgatcg ctcctgaatc ttcgcgacct cgggatgccc
     2761 cggtgggcga tcgagtacta ctgccgcgtc gcccgcgtgg cgggtcttcc gccccacatg
     2821 gtcgtgtgcc atgtggcggt ggtggttgcc ggccggcagc tgcacgcgcc cacccccccc
     2881 gaacaggctg tgaagcccgc tgtggatggc gagagccgcc ccgaggagga ctcgagcaac
     2941 ctggcgatcc tacggctggt gcaggccgac acggcacggg cagagccaga gccgcgaccc
     3001 cggcggcgca gcagaactcc ctaggccagc gcgggcttct gcccggcgta cccccgcagt
     3061 cggaaaccca gcaccagcag caacaccccg aacaccagcg cgtaggtccc gatgagccag
     3121 accaccgcca gggccccggt cgcgggacgg aagaggagca gcagcccg
//
LOCUS       DASQGH010000637         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5637, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000637 DASQGH010000000
VERSION     DASQGH010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5637"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VEW91_12735"
     CDS             <1..675
                     /locus_tag="VEW91_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYM92487.1"
                     /translation="QIHVAEGKYEVEMIAREHGQPQLHWLDLLGVVTDRMVAIHCVWL
                     AGDEIRVMGARGARLAYCPASNMFLGDGITNIPAMLDAGVTVGLGTDGPCSNNRASIF
                     DEMRTASLLQKVSTLSGETLPAETALAMGTRNGGAVLGLPVGEIAPGLRADLVALSLR
                     DLSLQPPGPVLKHVVPALHPAAIVRVVVAGRTVVQNGRLLTIPQDEIVDHVARATVRS
                     GLFPSN"
     gene            781..1686
                     /locus_tag="VEW91_12740"
     CDS             781..1686
                     /locus_tag="VEW91_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM92488.1"
                     /translation="MNDSAERHAGLTRRGLLAGTLGTVGAALLRPHDASRASAAPAGM
                     APEEEKLYADARKEGKVVWWTAHYQLSAAESVRDAFVARYPGIEVQFIRQTAQVVYQR
                     LTQDLKAGVHELDVFASTDEAHYLTLKKQGVLAPYVPLGVTNLPKTYRNIDPDNTYHM
                     GALGFVVINYNTKLGSPPQKWTDLLDGRYRGQITIGHPGFSVYVGNWVVAMWDKYGWE
                     YFTKLAKNNPKIGRSINDTVTDIVSGERLVGAGPDNTSLENKAHGNPIDIQLPTDDAI
                     LIASPVAVLKDAPHPNAARLFESFF"
BASE COUNT          330 a          594 c          554 g          263 t
ORIGIN      
        1 cagatccacg tagccgaagg caagtacgag gtcgagatga tcgcccggga gcacgggcag
       61 ccgcaactgc actggctcga cctcctcggc gtcgtgaccg accgcatggt cgcgatccac
      121 tgcgtctggc tggcaggcga tgagatccgg gtgatgggcg cgcgcggcgc caggctcgcg
      181 tactgccctg cgagcaacat gtttctcggc gacggcatca ccaacatccc cgcgatgctc
      241 gatgcgggcg tcaccgtggg actcggcacg gatgggccgt gcagcaacaa ccgcgcgagc
      301 atcttcgatg agatgcggac cgcatcgctc ctccagaagg tctcgacgct gagcggggag
      361 accctcccgg ccgagacggc actggcgatg gggaccagga acggcggcgc ggtgctcgga
      421 ttgcccgtcg gcgagatcgc gcccgggctg agggccgacc tggtggccct cagcctcagg
      481 gacctctccc tccagccgcc ggggccggtg ctcaagcacg tcgtgcccgc gctccacccc
      541 gcggcgatcg tccgggtggt ggtggccggc cggacggtgg tgcagaatgg gcgcctgctc
      601 accattcccc aggacgagat cgtcgaccac gtggcgcgcg cgaccgttcg cagcggcctc
      661 ttcccctcca actagctctg ctgctacgtg agtgcccttc gcaagaagga gtttggggat
      721 ttccgtggga agccccgcgt aacgccccat tcgacggaac ggcagaaccg gagggccgac
      781 atgaacgact ctgctgagcg gcacgcaggc ctgacgcgcc gaggtttgct cgccggaacc
      841 ctgggaacgg tcggcgccgc gctcctcagg ccccacgatg cctcgcgggc gtcggccgcc
      901 cccgccggga tggccccgga ggaagagaag ctctacgccg acgcgaggaa ggaaggcaaa
      961 gtcgtctggt ggacggcgca ctaccagctg agcgcggcgg aatccgtgcg cgacgcgttc
     1021 gtggcgcggt acccgggcat cgaggtccag ttcatccgcc agaccgcgca ggtcgtctat
     1081 caacgcctga cccaagacct caaggcgggc gtgcacgagc tcgatgtgtt cgccagcacc
     1141 gatgaagcgc actatctcac gctgaagaag cagggcgtgc tggcccccta tgtccctctg
     1201 ggggtgacca atctgcccaa aacgtaccgc aatatcgatc ccgacaatac ctaccacatg
     1261 ggagcgctcg gcttcgtcgt gatcaactac aacaccaagc tgggaagccc gccacagaag
     1321 tggaccgacc ttctcgacgg gaggtaccga ggccagatca ccatcgggca ccccgggttc
     1381 tcggtctacg tcggcaactg ggtcgtggcc atgtgggaca agtatgggtg ggagtatttc
     1441 acgaaactgg ccaagaacaa ccccaagatc ggccgctcga tcaacgacac cgtgacggac
     1501 atcgtcagcg gcgaacggct ggtgggagcc ggcccggaca acacttccct cgaaaacaaa
     1561 gcccacggga atcctatcga tatccagctc ccgaccgacg acgcgatcct catcgcctcg
     1621 ccggtcgccg tcctgaagga cgccccgcat ccgaacgccg cccgcctctt cgagagcttc
     1681 ttctaaactc gagagtattc gatcgcgatc gctaagacct acagctaccc aatccgcccc
     1741 g
//
LOCUS       DASQGH010000638         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5659, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000638 DASQGH010000000
VERSION     DASQGH010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5659"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1636
                     /locus_tag="VEW91_12745"
     CDS             209..1636
                     /locus_tag="VEW91_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYM92489.1"
                     /translation="MDATDLCFTPATELVRLISTRRVSPVEVTEAVLRQIERLNPRLR
                     AFLTVTDDRAREEAKAAEARALKRGPAGPLDGVPYSIKDLEPTAGIRTTYGSKFFEHN
                     VPEEDGVVAGRLRTSGGVLLGKTNTPHFGHKDMCDNLLGPPCRNPWKLDRTSGGSSGG
                     AAAAVAAGLGPLAQGSDGAGSIRIPSALCGVFGLKPSFGRVPYHPNADYWSARSHNGP
                     LARTVRDAALMLNVMAGADARDPLTIDAAPEDYVKACDGELKGLRVAWSPDLGYAAST
                     VASEVKAIAERAARRFSELGCRVEAPALRWPDPRGFHKIIWEVSIASRYGDRAIERPE
                     WIEPSLMQMFLNAGRLSAIEYGKALVARSAFYQAVRTFFDTYDLLLTPQMPVAAWSVD
                     AGADGGPLDAEGRPMRFLDRVPFMYPFNFTGQPAANVPCGFTAEGLPVGLQIVGRWHA
                     DALVLRAAAGFEAVQPWAGRRPALD"
     gene            complement(1688..2359)
                     /locus_tag="VEW91_12750"
     CDS             complement(1688..2359)
                     /locus_tag="VEW91_12750"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HYM92490.1"
                     /translation="MAVQERILQSWSGGKDSCMTLVEILRDGTRSVAALLTTVTEGYD
                     RVSMHGVRHALLDRQAAALDLPLETVYIPQNATNEAYQARMEAVFERYRQEGITTIAF
                     GDLFLADIRRYREQWLAHAGMTPVFPLWMRDTRELARRFVDEGFEAVVTCVDTRVLDR
                     GFAGRRFDHALLADLPQAVDPCGENGEFHTFVHGGPIFRQALRVARGEIVQRESWCFC
                     DLVPA"
BASE COUNT          397 a          877 c          879 g          414 t
ORIGIN      
        1 gtcctgcaca tcatggggcg cgagaagggc catccccaca ccctcacccc gtgggcgcac
       61 gtcgaagggc gccgggtctc gtatccgggc gctccgggcg acgccggggc cgggtctcgg
      121 gcccccgcag cgcgcgcccc gcggcccgcg caggggcgtc tcttgtaagc gaagggccaa
      181 acgcaagact cttacgaagg agaccagcat ggacgcgacc gatctgtgct tcacgcctgc
      241 aacagaactc gtgcggttga tcagcacgcg gcgggtctcg ccggtcgagg tgaccgaggc
      301 cgtcctcagg cagatcgaac ggctcaaccc gaggctgcgt gcgttcctca ccgtgacgga
      361 cgaccgggca cgtgaggagg cgaaggcggc cgaggcccgg gccctcaagc gggggcccgc
      421 gggcccgctc gacggggtcc cgtactccat caaagatctc gaaccgacgg ccggcatccg
      481 caccacctac ggctccaaat tcttcgagca caacgtcccc gaggaggatg gcgtggtcgc
      541 cggccgcctc cggacgtcgg ggggcgtcct cctgggaaag acaaacaccc cgcactttgg
      601 ccacaaggac atgtgcgaca acctgctcgg cccgccgtgc cgaaatccct ggaagctcga
      661 ccggacatcc ggaggatctt cggggggcgc ggccgcggcg gtggccgccg gattggggcc
      721 gctggcgcag ggctcggacg gcgcgggttc gatccgcatc ccttcggcgc tgtgcggggt
      781 gttcgggctc aagccctcgt tcggccgggt gccgtatcac ccgaacgcgg actactggtc
      841 cgcccggtcg cacaacggcc cgctggcccg gaccgtgcgc gatgccgcct tgatgttgaa
      901 cgtcatggcc ggggcggatg cccgggatcc cctcaccatc gacgccgccc ccgaggacta
      961 tgtcaaggcc tgcgacggcg agctcaaggg gctccgggtc gcctggagcc cggacctggg
     1021 ctacgcggcg agcacggtcg cctccgaggt gaaggcgatc gcggagcggg cggcgcggcg
     1081 gttttccgag ctcggctgca gagtcgaagc gccggcgctc cggtggcccg atcctcgagg
     1141 attccacaag atcatctggg aagtgagcat cgcgtcgcgg tacggggacc gcgcgatcga
     1201 gcggcccgag tggatcgagc cgtcgctcat gcagatgttc ctcaacgccg ggcggctcag
     1261 cgccatcgag tacgggaagg ccctcgtcgc ccggagcgcg ttctaccagg ccgtgcgcac
     1321 gttcttcgac acgtacgacc tgctgttgac gccccagatg ccggtcgcgg cctggtccgt
     1381 cgacgcgggt gcggacggcg gcccgctcga tgccgagggc cgcccgatgc ggttcctcga
     1441 tcgggtgccc ttcatgtatc cattcaactt cacgggccag ccggccgcga acgtgccctg
     1501 cggattcacg gcggaaggcc tgccggtggg cctgcagatc gtcggccggt ggcacgccga
     1561 cgcgctcgtg ctgcgcgcgg cggccgggtt cgaggcggtg cagccgtggg ccgggcgccg
     1621 gcccgcgctc gactgacgcg aacgccctgc gtcgtctcgc taccgctccc cgtctagcgc
     1681 ggtgccgcta ggccggcaca agatcgcaga agcaccacga ttctctttgc acgatctccc
     1741 cgcgcgcgac gcggagggcc tgacggaaga tgggcccgcc atggacgaag gtatggaact
     1801 cgccgttctc gccgcacgga tcgaccgcct gcgggaggtc cgcgagcagg gcgtgatcga
     1861 aacgcctgcc ggcaaatccg cgatcgagca cccgggtatc gacgcaggtc accacggcct
     1921 cgaatccctc gtcgacgaac cggcgggcca gctcacgggt gtcgcgcatc cagagtggga
     1981 acaccggcgt catccctgcg tgcgccagcc actgctcccg gtagcgccgg atgtcggcca
     2041 ggaaaaggtc gccaaacgcg atcgtggtga tcccctcctg ccggtatcgc tcgaacaccg
     2101 cttccattcg cgcctggtac gcttcgttgg tggcgttctg cggaatgtaa acggtctcga
     2161 gtgggaggtc cagcgcggcg gcctgccggt cgagcagggc gtggcggacg ccgtgcatgc
     2221 tgacccggtc gtacccttcc gtgaccgtgg tgaggagcgc cgccaccgac cgggtgccgt
     2281 cgcgcaggat ctcgacgagc gtcatgcagc tgtccttgcc gccgctccac gattggagga
     2341 tccgttcctg cacagccatg cagaagagtg ctaccgggca gaggagcccg cttcctgccg
     2401 atcagaggcg gccgagccct ctaaatcgcg attattgttt tcttcatggt gttcttagtt
     2461 ctggtttccc acgatgaata atccatacgg caggggttga tcgaaaattg ccactgccga
     2521 tttctcatct ttcctttgta gcttttctgg ggtgtcaatc gactccc
//
LOCUS       DASQGH010000639         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_5661, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000639 DASQGH010000000
VERSION     DASQGH010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5661"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VEW91_12755"
     CDS             <1..314
                     /locus_tag="VEW91_12755"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYM92491.1"
                     /translation="QRCEQLAIRTVLVVNELNGPEGTDFGLVFAVPEADAIVSTGNKD
                     EIIRLAPVSEVVGGDTIWDWRSYDDPVEGNVRDGFSTSLRRLYCSTTQLGAESLTSAA
                     F"
     gene            439..1716
                     /locus_tag="VEW91_12760"
     CDS             439..1716
                     /locus_tag="VEW91_12760"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HYM92492.1"
                     /translation="MRLEVATYRVSALEWGPQTALDGARLSVNRSEIEHLVRNPEAFA
                     SSAVDIARPGERVRLLNVIDAVGPLLKVAGDSCAYPGVAGPAVTAGSGITHRLDGVSV
                     VTCGVPPKPPTGAQSTKPAFIDMFGDAAPYCDASETRNLVISTSPVPALSNEDYDAAV
                     REAAHRVALRLAETTRTRPPDAVDTFELGPADGLPRVVCIMQLMDQGPLIRTLLYGHH
                     LGEDFVPTLIHPNELLDGAVVNANHRSCLKQSTRMHARNPVVLELYRRHGRDVALAGV
                     VLTRGSNANHFLKERSAMYAAKLAAMLGAEAAVAMMEDTGNSIIDYMLTIRALERRGI
                     RTVGVMHELAGPQGDNSPLLDAVEEADALISSGNVDELFPTGRPERLIGGSVFRYYSG
                     TTADPHDAVRVSAMECFCGLARMALAGFTAIEH"
     gene            1754..3082
                     /locus_tag="VEW91_12765"
     CDS             1754..3082
                     /locus_tag="VEW91_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2801..2803,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HYM92493.1"
                     /translation="MATFRIVHYLNQFYGGIGGEAEADTPLAVREGPVGPGRLLEQTL
                     RGGGRVVATLICGDNAFHERRDEVLSQIVNSAREWAPDALIAGPAFASGRYGLACAAV
                     AARVGSELGIPTVTGMHPGNPGAQPTDPPIYIVPTGETSVDMESALDGMARLARKLIS
                     GERLGPAKTEGYLPRGIRRNARLQRPAAARAVDLLLEKLAGRPIDTELPIEVRDRVSP
                     PPPLSDLSAATIALVTEAGVVPRGNPHGIEAARATRWARYSIEGMDGLRAGDYESIHG
                     GYDNAWVHQDPNRVLPVDALRALEREGVIGRLHPEYFATCGSIGNPTEMRRMGREIAR
                     EVAAAGVSGVILPATUGTGTRSGATLAKEIERIGIPAVVCTCLFTVATRVGANRILRA
                     GRFHHPFGDPNRTPEGELLWRRQVVEAALRALTTPVDRSTTFEVTEYVMR"
     gene            3124..3798
                     /locus_tag="VEW91_12770"
     CDS             3124..3798
                     /locus_tag="VEW91_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYM92494.1"
                     /translation="MNEDQVYVALQAARGRMSRREFLRQGTLLSMSVPTMLALLDRPP
                     AAEAATKVVNLAGYGGVSNDVIRKAWAEPFEKETGIKVNMGANASLALAKLQVQSGNP
                     QWDIVGLDGSELPTAVKQNLLERIDPQKVDMSKVPPYLKTPYGYKYAVYFFTKVYDQR
                     KIPDDNAPRTWAEFWDTARYPTKRSVYQNVADGSVLEAALMADGVPISQVYPLDVERA
                     LKSLDR"
BASE COUNT          646 a         1198 c         1350 g          630 t
ORIGIN      
        1 gccagcggtg tgagcagctt gccatccgga cggtcctcgt cgtcaatgag ctcaacgggc
       61 ccgaggggac ggacttcgga ctcgtgttcg cggtccccga ggccgatgcg atcgtgagta
      121 cagggaataa ggacgagatc atccgcctcg ccccggtgtc ggaggtggta ggcggggata
      181 ccatctggga ctggcggagc tacgacgacc cggtggaggg gaatgtccgg gacgggttct
      241 cgacctccct gcgccggttg tactgctcga ccacccaact cggggcggag tcgttgacgt
      301 cggcggcgtt ctagggcgtt ctagatggtt ttcctcctga cgcgcgaccg gtgtggctgg
      361 aggaggggtg aggcccgctt ccgaatcgtc agaggacctt cgggtgccca gccgggcgaa
      421 gacaagggaa ggatgccgat gcgtcttgag gtggcgacgt accgggtttc agcactggaa
      481 tggggacccc agacggcgct cgacggagcg cgtctctcgg tgaaccgatc cgaaatcgaa
      541 cacctcgtgc gcaatcccga ggccttcgcg tcctcggccg tggacattgc tcgcccgggc
      601 gaaagagtgc gtctcctcaa tgtcatcgac gccgtcgggc ctcttctgaa ggtcgcgggt
      661 gattcctgcg cgtatccggg cgtggccggt cccgccgtca cggccgggtc ggggatcacg
      721 catcgtctgg acggtgtttc cgtcgtgacc tgcggtgtgc ccccgaagcc gccgacgggg
      781 gcgcagagca ccaagccggc cttcatcgat atgtttggcg acgccgcgcc ctactgcgac
      841 gcgtccgaga cccggaacct ggtgatttcc acgagtccgg tacccgcgtt atcgaacgag
      901 gactacgacg ccgccgtgcg tgaggccgct caccgcgtgg cgctccggtt ggcggagacg
      961 acgcgcaccc ggccgcctga cgctgtggac acgttcgaac tcggtccggc cgacggcctg
     1021 ccgcgggtcg tttgtatcat gcaacttatg gaccagggtc cgctgattcg tacgctgctc
     1081 tacgggcatc atctgggtga ggatttcgtc ccgacgctga tccaccccaa cgaacttctc
     1141 gacggagccg tcgtcaatgc caaccaccgc agctgcctga agcagtcgac gcgcatgcac
     1201 gcccggaacc ccgtggtcct tgaactctat cgccggcacg gccgcgatgt ggcgttggcc
     1261 ggggtcgtcc tgacacgggg ctccaacgcc aaccactttc tgaaggagcg gtcggccatg
     1321 tacgctgcga agctcgccgc catgcttggg gcggaggcgg cggtcgccat gatggaagac
     1381 acgggcaact cgatcatcga ctacatgctc acgatccgtg cccttgaacg tcgcgggatc
     1441 cgcacggtag gcgtgatgca cgagctggca gggccgcagg gcgacaattc cccgctcctc
     1501 gacgctgtcg aggaggcgga tgcgcttatt tcgagcggga acgtcgacga gctgtttcca
     1561 accgggcggc cggagcgcct gatcgggggc agcgtgtttc gttactacag cgggacgacg
     1621 gcggatccgc acgacgcggt gcgggtctcg gccatggagt gcttttgcgg actcgcgcgc
     1681 atggcgcttg cgggctttac ggccatcgag cactagacgt gctccgcagg tcggatcggg
     1741 gaggtgacgc acgatggcca catttcgcat tgtgcactac ctgaaccagt tctacggagg
     1801 catcggtggc gaagcggagg cagacacgcc gctcgcggtg cgcgaagggc ccgtggggcc
     1861 gggaaggctg ctcgagcaaa cgcttcgcgg gggcggacgc gtggtggcga cgcttatctg
     1921 cggggacaac gccttccacg agcgccgcga tgaggtgttg tcgcagatcg tcaactccgc
     1981 tcgggagtgg gccccggatg cgctcatcgc cgggccggcg ttcgcttcgg ggcgatacgg
     2041 tctggcgtgt gcggccgtcg ccgcccgggt gggctcggag ctgggcatcc ccacggtgac
     2101 gggcatgcat ccgggaaacc cgggcgcgca gcccaccgat ccgccgatct atatcgtgcc
     2161 cacgggcgag acgagcgtgg atatggagtc cgcgctcgat ggcatggcgc gattggcccg
     2221 gaagctgatc tccggcgagc gcctcgggcc ggcgaagacg gaaggatatc tgccgcgcgg
     2281 catccgccgc aacgcgcgcc tccaacggcc cgcggccgcg cgcgccgtcg atctcctgct
     2341 ggaaaagctg gcgggccggc ccatagacac cgaactgcct atcgaggtgc gcgaccgggt
     2401 atccccgcca cctccgctct ccgatctgag cgcggcgacg atcgccctgg tgacggaggc
     2461 gggcgtggtt ccgaggggga acccccacgg catcgaggcc gcccgggcca cacggtgggc
     2521 gaggtactcc atcgagggga tggatgggct ccgggcgggg gactacgagt cgatccacgg
     2581 cggatacgat aatgcctggg tgcatcagga ccccaaccgc gtcttgcccg ttgacgcgct
     2641 gcgggcgctg gaacgagagg gggtgatcgg acgcctccac cccgagtact tcgccacgtg
     2701 cggcagcatc ggcaacccca ccgagatgcg ccgcatgggc cgggagatag cccgcgaggt
     2761 ggccgcggcg ggggtgagcg gcgtcatcct gccggcgacc tgagggacag gtacgcgtag
     2821 cggcgctacg ctcgcaaagg agatcgagcg gatcggcatc ccggcggtcg tctgcacctg
     2881 tctgttcacg gttgccacac gcgtgggggc gaaccggatt ctgcgggccg gccgtttcca
     2941 ccaccccttc ggcgacccga accgtacccc ggagggcgag ctcctctggc ggcgtcaggt
     3001 ggtggaggcc gccctgcgcg cgcttacgac cccggtcgac cgatcgacca cgtttgaagt
     3061 gaccgagtac gtgatgcggt agtgcgaaca cggcggtggc gagatcaaca gaggggggcg
     3121 acgatgaacg aggatcaggt ctacgtggcg ctgcaggcgg cccgcgggcg aatgagccgg
     3181 cgcgagttcc tcaggcaggg gacccttctg agcatgtcgg tgcccacaat gctggccctc
     3241 ctggaccgtc ccccggccgc tgaggccgcg accaaggtcg tgaaccttgc cgggtacggc
     3301 ggcgtttcga acgacgtgat ccgcaaggcc tgggcggagc cctttgagaa ggagactggg
     3361 atcaaagtca atatgggcgc gaacgcatct ctcgccctgg ccaagctgca ggtgcagtca
     3421 gggaatccgc agtgggacat cgtggggctc gacggcagtg agctgccgac ggccgtcaag
     3481 cagaatctgc tggagcgcat cgaccctcag aaagtcgaca tgtccaaggt gccgccgtat
     3541 ctgaagaccc cttacggata caagtatgcc gtctacttct tcaccaaagt gtatgatcaa
     3601 cgaaagattc ccgacgacaa cgcgccccgg acgtgggcgg agttctggga taccgcgcgc
     3661 taccccacga agcgttcagt ctatcagaat gtcgcggacg ggagcgtgct cgaggcggcg
     3721 ctgatggcgg acggcgtgcc gatttctcag gtctacccgc tggacgtgga gcgggcattg
     3781 aagagcctcg acaggtaggg aaagagcaac attatctggc acag
//
LOCUS       DASQGH010000640         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U11801 k141_56532, whole genome
            shotgun sequence.
ACCESSION   DASQGH010000640 DASQGH010000000
VERSION     DASQGH010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945526
            Sequence Read Archive: SRR6788485
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 09:00:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,556
            CDSs (total)                      :: 2,528
            Genes (coding)                    :: 2,497
            CDSs (with protein)               :: 2,497
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 24 of 31
            Pseudo Genes (internal stop)      :: 4 of 31
            Pseudo Genes (multiple problems)  :: 3 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56532"
                     /isolate="SMAG_U11801"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.19 N 81.42 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VEW91_12775"
     CDS             complement(<1..255)
                     /locus_tag="VEW91_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYM92495.1"
                     /translation="MTKKEQQRAHVLSRVQAGALTARTAAELLTLSIRHVRRLLAEVR
                     QHGLAALAHGNRGRPSPRRLAEAIRTKVLALARTTYAGVND"
     gene            complement(381..>1685)
                     /locus_tag="VEW91_12780"
     CDS             complement(381..>1685)
                     /locus_tag="VEW91_12780"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HYM92496.1"
                     /translation="LLAEVRQHGLAALAHGNRGRPSPRRLAEAIRTKVLALARTTYAG
                     VNDHHLTELLAEREGLHLSRPLVRLLLRKAGIGSPRTRRPPRHRRRRERMPQAGLLVQ
                     MDGSHHAWLEARGPRLVLLSAIDDATNRVLGAVFRLAEDAQGYFLLLRQMIRTYGLPV
                     ALYTDRHGIFHRDPHTPVTLDEQLRGAPELTQLGRALHELGIRWIPASSPQAKGRIER
                     LFGTFQDRLVSELRVARAATLAEANLVLRAFLPRFNARFARPPAQPRSAFRPGPAGQA
                     LDTLCCFKYARTVANDNTVGLAHHRFQLLPGPGGRSYAKARVEIHERLDGSFAVYQHG
                     QRLASRALTPVPSRTAPTLRARDYTRLATAPRPRNGTTHAASPPARPPATPRDDAGSA
                     APPTGHAKRAKPAPDHPWNWYALEAVRRKALRQAGVTFSLIR"
BASE COUNT          252 a          479 c          690 g          264 t
ORIGIN      
        1 atcattgacc ccggcatagg tggtgcgagc caacgcgagg accttggttc tgatggcctc
       61 cgcgaggcgc cgtggggacg ggcggccccg gttgccgtgg gccagcgctg cgaggccgtg
      121 ctggcggacc tcggccaaga gtcggcggac gtgccggatc gacagggtca agagctcagc
      181 cgctgtccga gcggtgaggg ccccggcctg gacccgggac agaacatggg cgcgttgctg
      241 ctccttcttg gtcaaggtaa tcgtctccct gatcatactg ccagggtgac agattcacgt
      301 agccattaag gggtgacaga ttcacgtagc taatacaccg ggccgcggcg cttgtgtcgt
      361 agatcagtga tcctgtcacc ttaacggatt aatgaaaatg tcaccccggc ctgtctgagg
      421 gccttgcgtc tgacggcttc tagcgcatac cagttccaag ggtgatcggg tgctggtttc
      481 gcgcgcttag catgcccggt gggcggggca gcgctgccgg cgtcgtcccg gggagtcgct
      541 ggaggcctgg ctgggggcga ggcagcgtgt gtggtgccat tgcgggggcg aggggcagtg
      601 gcgaggcgtg tgtagtctcg cgcccggagg gtcggcgcgg tccgcgaggg tacgggggtc
      661 agggcccggc tggcgagccg ctgcccgtgc tgatagacgg cgaaggagcc atccaggcgt
      721 tcgtggattt cgacgcgggc tttggcatag ctgcggcccc cggggccggg gaggagttgg
      781 aagcggtggt gagcgaggcc gacggtgttg tcgttggcca ccgtgcgcgc gtatttgaag
      841 caacagagcg tgtccagggc ctggccggcc gggcctgggc gaaaggcgga gcggggctgc
      901 gcgggcggcc gggcgaagcg ggcattgaag cgggggagga aggcccggag cacgaggttg
      961 gcctccgcca aggtggcggc ccgcgccacg cggagctcac tgacgaggcg atcctggaag
     1021 gtgccaaaga ggcgctcgat gcggcctttg gcttgggggg aggaagcggg gatccagcgg
     1081 atgccgagtt cgtggagggc gcggccgagc tgggtgagct cgggcgcgcc gcgcagttgc
     1141 tcgtcgaggg tcaccggggt gtggggatcc cggtgaaaga tcccatgccg atcggtatac
     1201 agcgcgaccg ggagcccata ggtccggatc atctgccgca ggaggaggaa gtagccctgg
     1261 gcatcctccg ccaggcgaaa gacggcgccc aggacgcgat tggtggcatc gtcgatcgcg
     1321 ctgagcagga cgaggcgggg cccgcgggct tcgagccagg cgtggtggct gccatccatc
     1381 tggacgagga ggccggcctg gggcatgcgc tcgcgccggc ggcgatggcg cggggggcgg
     1441 cgggtacggg ggctgccgat cccggccttg cggaggagca gccgtaccag cggccgcgag
     1501 aggtgcaggc cttcccgctc cgcgagcaac tcggtgaggt gatgatcatt gaccccggca
     1561 taggtggtgc gagccaacgc gaggaccttg gttctgatgg cctccgcgag gcgccgtggg
     1621 gacgggcggc cccggttgcc gtgggccagc gctgcgaggc cgtgctggcg gacctcggcc
     1681 aagag
//
